LOCUS       DAOTOW010000001        12927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_4660_length_12927_cov_3.478947, whole genome shotgun sequence.
ACCESSION   DAOTOW010000001 DAOTOW010000000
VERSION     DAOTOW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_12927_cov_3.478947"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(646..720)
                     /locus_tag="P5243_00005"
     tRNA            complement(646..720)
                     /locus_tag="P5243_00005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Pro,seq:ggg)
     gene            complement(777..1037)
                     /locus_tag="P5243_00010"
     CDS             complement(777..1037)
                     /locus_tag="P5243_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17857.1"
                     /translation="MRNIGIVLLLILLSVVSCKQAKTFDKQQCMKDADVQAIYKEQTQ
                     AYCNCVEQSITQLGDTIVVNDSVIEAHKQACANEFTTLDTNF"
     gene            1186..2118
                     /gene="trxB"
                     /locus_tag="P5243_00015"
     CDS             1186..2118
                     /gene="trxB"
                     /locus_tag="P5243_00015"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRS17858.1"
                     /translation="MNFEKVKCLIIGSGPAGYTAAIYAARANMNPVLYEGLQPGGQLT
                     TTTEVDNFPGYPEGVTGPQLMEDLRKQAQRFGTDVRSGYATAVDFSGTNKIVEIDGEK
                     KIEAQVVIISTGATAKYLGLESEEKYKGMGVSACATCDGFFYRKQDVAVVGGGDTAAE
                     EATYLAGLANKVYLIIRKDHMRASKAMQKRVENTPNIEVLYEHVTLEVLGDANGVNGV
                     RLLKKGTEEVTINVTGFFLAIGHTPNTEIFKNFIELDEVGYIKTKPGSTHTNIPGVFA
                     CGDVQDPHYRQAITAAGSGCMAALDAERYLGSLL"
     gene            2165..2710
                     /locus_tag="P5243_00020"
     CDS             2165..2710
                     /locus_tag="P5243_00020"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRS17859.1"
                     /translation="MKIYTKSGDKGTSSLLGGTRVAKYHDRLECYGTIDELNSHIGVV
                     RDLCNNNAVCEILAHIQHTLFTIGSYLANEKPHERTTPILSTQHIELLEKTIDTYTEE
                     LPKLTHFILPAGDIVSSHTHVCRTVCRRAERLIVKLSESTHIEENVIIYMNRLSDFLF
                     VLARKFLKDVNKQEITWNPLL"
     gene            2763..2984
                     /locus_tag="P5243_00025"
     CDS             2763..2984
                     /locus_tag="P5243_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRS17860.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDFAIRSGAPLEVIENLQEIE
                     DEGEVYESIEDIWPDYPSKDDFFFNEDEY"
     gene            3038..3904
                     /locus_tag="P5243_00030"
     CDS             3038..3904
                     /locus_tag="P5243_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17861.1"
                     /translation="MDYLWHILKELLTTTLLQIWIVFGIFFILGLLLYFFSRSTRKVF
                     VTSGYPKIDIFITGWIGTPVHELGHALFCVLFGHTIQDMKLFKPDKADGTLGYITHSY
                     DKKNLYHTVGNFFIGCGPILLGSAMLFILFYLGFPLSHEIATVFNTYGNIYISYNTII
                     ESLQQIVTLVWHMFSVILTKQHINSWQFWVIVYIAFSIASHMQLSPPDIKIMLKGFLV
                     ILGLLTVVNIVAVVWEFDINAWIVVHFKIGYIYGMLVFATSISAVFFIVSYICMAIVY
                     GISKHKLLPLFG"
     gene            complement(3887..7069)
                     /locus_tag="P5243_00035"
     CDS             complement(3887..7069)
                     /locus_tag="P5243_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS17862.1"
                     /translation="MNRIIIFVVFWLVSLNSGNAQFFCNFQNYARHDGLSDEFIFSLS
                     QDTTGYIWMATSYGINRFDGLLFHSFIKSEHQYQSVLRNDFNCVFTDSKGRIWFGSHN
                     GTIMMYNQEKDQFQNVSLDFEYHLEYPSISRFYESHNKLYALTSHGVLLYNEQSGTFN
                     FAFQEFPVFKQIHVISMIQDDAGNFWFGTAKSGLLYVDAQQKTVKVFPIQDELGILRI
                     PSFCKINKNLIYIGTSRGIYELQPSNHIIVKSTYFNTVEQNYISSIVRDSSHNIWIGT
                     NYNGLWVYDANNLFYKIEDYQTEGIKIASVNAILCDKENSIWVATQGNGLFLYNPSKN
                     KVQHTSMEVGLAHKVVSAITEDGNGNLWIGTDGCGISIFSPYYGYLSEIQTPYIPSNS
                     VLAFAKRTENEVWACMWASGIMKIDMITKRVTPYNLSQTEITNNLVKTICFYDANTLW
                     VGTYGNGVQEFDIPSGQLKRKIIALDTLFKTDRQKFINQIIRDRNGAMWVASLRTVYK
                     ITANNSLLVLNFDNDKLSFFPGYVHTLTEDFAGNIVIGTNRGLFVYSNQGELLEELSK
                     TIPELQGTEVLSLHCSANKQYWIATNNGLIVYDVSTKKYEIYYVDNYSKGNFYTSRAV
                     YQDTQGRTHWGTMNGLYSFYPNSMQQKHSILQFLFSDLFISYQKQSPNTDWYPTHISQ
                     LKELTISYKQNIWGVSFDAICYNAPDAVVFSYKLEGFDRDWNTIGNKREITFTNIPPG
                     RYVLFVKAWINNQDEAKVIQLSVHILPPWWKTWWFKILLLLFITAILYGIYYIRIYTL
                     KSQKEKLEAEVKRQTQSIIEQKKYIEEQNVELQQANDTKNYLFSIVAHDLRNSFTSLH
                     GFSELLHEEYETIQEKEKRNYIALITQSATVIYNLLENLLSWSRSQTRSIQCNPEMCQ
                     LYLLADRVVELYALAAQEKQIQLCNTIPKDLYVFVDKEMLITAMRNICNNAIKFTPNN
                     GKVCFEAVETHTYITVTISDNGAGISPEEIQTLFTIKPSQSYKRKKNQGSGLGLIVSK
                     EFIEKNNGTIWVTSQIGVGSTFHIQLPKQG"
     gene            7280..8893
                     /locus_tag="P5243_00040"
     CDS             7280..8893
                     /locus_tag="P5243_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS17863.1"
                     /translation="MITVSNLAIQFGKRTLFQDVNLKFIPGNIYGVIGANGAGKSTFL
                     KILSGEIESTRGSVSLEPNERLSVLKQNHFEYDECTVIQTVLMGYKELWNIMNQKDAL
                     YAKPDFSDEDGLLAADLEEKFAEMNGWNAESDAAMLLSGLGIAESHHNKQMKELSGNE
                     KVRVMLAQALFGTPDNLLLDEPTNDLDVETVMWLENFLSNFDKTVLVVSHDRHFLDAV
                     CTHTVDIDYGKIQLFAGNYSFWYESSQLALRQQQQQNKKAEEKKKELLEFIARFSANV
                     AKSKQTTSRKKMLEKLNVEEILPSTRKYPGIIFKPSRDVGNKILEVHGLSAKMEGDVL
                     FKQVDFTVEKDDKIVFLSKDPRAMTALFEIISGNKKPDGGHFDWGVTVTSAYLPLDNS
                     QFFNTDISLLDWLAQFSDDTHEIFLRGYLGRMLFSGEDVYKKSSVLSGGEKMRCMISR
                     MMLKDANVLILDSPTNHLDLESIQAFNNSLMQFQGIVLMSSHDHEFIQTVCNRIIELT
                     PNGIIDKRMDYDDYIIDEKVKELRNKLYK"
     gene            8900..10204
                     /locus_tag="P5243_00045"
     CDS             8900..10204
                     /locus_tag="P5243_00045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS17864.1"
                     /translation="MTFDEFNIHEQLLEAISYMNYEKASPIQELAIPPALLGHDILAC
                     AQTGTGKTATFVIPIIHDIVTNKLEGTTSLILVPTRELAIQIHQEIQGFSYFTDTTSK
                     PIYGGDKGADWDAQKRALTEGTNIIIATPGRLLAHLKLGYVDFKQIQHFVLDEADRML
                     DMGFYDDIQQILSYIPQQRQTLFFSATMPPAIQKLADKILQNPKEIRISISKPAEGVT
                     QKAYLAYPHQKNGVIQHILQQNPSYDSIIIFTSTKRMVQKIVHDIARKNPGITVEGIS
                     SDLEQKEREEVLLKFRSKNTKILVATDVMSRGIDIKGINLVINYDVPGEAVDYVHRVG
                     RTARADSKGEAITLISDVDMYKFARIEALIEKEIPKLPIPAHLGEAPVWKSKQSKSFK
                     SKLQENKQQHKSQGDGNSSQKQKKKRFWRPKKKPNSQSNEAQ"
     gene            10191..10991
                     /locus_tag="P5243_00050"
     CDS             10191..10991
                     /locus_tag="P5243_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRS17865.1"
                     /translation="MKRNKGFWITIIILIVIGIGIYLYFNYIQAQPIPANNTYTSYKP
                     IPELEIPCGTQYIKHSHYAFEYKEEHEQSIWVAYMLTSKKNAQTYKRSNSFYPDPLVS
                     TETATNKDYSKSGYDKGHLAPAADMNWSEQAIKESFYFSNISPQLPAFNRGIWKTLEE
                     QVRTWAQKFDTLYIVTGPIVEPGLPTIGENKVSIPNYFYKAILVYSYSRAFGIGFILP
                     HAASNNSIYSFVVPIDSIESKIHRDLFCKLPDTIEEKIESSTTISDWK"
     gene            11076..12467
                     /gene="xseA"
                     /locus_tag="P5243_00055"
     CDS             11076..12467
                     /gene="xseA"
                     /locus_tag="P5243_00055"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRS17866.1"
                     /translation="MGTQSSITLLELAQAIELGIQTALPNSYWVVAEISELTVNSKGH
                     CFLELIEKPEHNETIIAKLRATIWSYKFQLLQSYFYASTGIHLQTGLKVLICVKVEFH
                     AVYGLSVSIHDIDPVYTIGEEEKQRNEIIARLHDEGIFTMNKDLKLPVFPQRLAVISS
                     AQAAGYQDFVKQIASNKNSLRVSITLFTAAMQGIETEQSIIKALNSIYEREHEFDAVI
                     IIRGGGAKTDLRWFDNYTIASHIAQFPLPVITGIGHDKDISITDMVAHTSVKTPTAAA
                     EFIISMGNALVSTIDNLHARLSDACNERIAEQKFTLQHILQELFHSNTLRTTIANNKL
                     NNCIQKIKSASLFTIQKQKHFIEHISSNISPAIHALTHKHQQNLIHSAYKLQIHSHNC
                     ILHNKQSLELLQTKIELHNPQRILSKGYSITTNAHGTIINSIDSVQPNDKLYTYIKDG
                     IITSTINKKDATN"
     gene            complement(12460..12840)
                     /locus_tag="P5243_00060"
     CDS             complement(12460..12840)
                     /locus_tag="P5243_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRS17867.1"
                     /translation="MTELEIKKTPSTPKVWYSSDEATLRIEGRSFPENARDFYGPIIQ
                     WIESNTSNLGSLFTVSINLEYYNTASSKFLMEIFHLLEKIQNTGDCKVTIQWHYMEED
                     DDMRDAGLEYKQFIHVPFELISFN"
BASE COUNT         4501 a         2233 c         2020 g         4173 t
ORIGIN      
        1 aataaatttt gttttattaa attaattttg tagttttatc ggacaataat gattaattat
       61 taaaaaaaca aacacgtcga catatattgg cgaactgact aactttattt gcattataat
      121 tttaatatat atgcaaatga aacgttttct ttttttatta tgtatttctg tattgtcttg
      181 tagtagtcaa gtacagattg agttaatcaa caataattta tttgttgatt ctaaaacaac
      241 gtcaattcga cctaagcagc agctattaat ctttcattat caataatttc gatattcaat
      301 gaattttttt cttattaaga aactaaccaa aaatgtattt tttgttctca taacatgatt
      361 ttttttacat tatattgata cacattgaat tctgttcacc aaaaagtcca ttaaattgat
      421 aacaaaatca tacaaaaggc atcaaaaaag tatccttata acccaatggt aatcattgtt
      481 ttaaatactt gctggtgaaa aaaataaaat aaaatattat ttgttcaccg aattactcac
      541 cgaatagatt gcgatttttt gtgttcaaat gaacacaatt gattacaaaa aagcctgaaa
      601 atcaaaagat tttcaggctt ttgttgaaat tgaattccga ctttgtcggg atgacccgat
      661 tcgaacgggc gaccccacgc cccccagacg tgtactctaa ccgggctgag ctacatcccg
      721 aatgctcaat tgtatgcaaa tgtattgttt atttcttttg caacaaaatt accatatcaa
      781 aaatttgtat ctaatgtggt aaattcattg gcacaggctt gtttgtgagc ttcaatcaca
      841 ctatcattta ctactatggt atctcctaat tgtgtaatac tttgttctac acaattacaa
      901 tatgcttgtg tttgctcctt ataaatagct tgcacatcgg catctttcat acattgttgt
      961 ttgtcaaatg tttttgcttg tttacaactt accacactca aaagtataag taacaataca
     1021 ataccaatat ttctcatttt tatatgttgt tttgtgtaat gaattgaatc tttataagac
     1081 aaatatctat atttttttaa aaaaatcaca ttgcaaaatg tgtaaaaaaa aactactttt
     1141 gttatatgct tacaaataaa aactaatcaa taaatataaa taaatatgaa ctttgaaaaa
     1201 gtaaaatgtt taattattgg cagtggacct gcgggttata cagctgctat ttatgctgct
     1261 cgtgccaata tgaatccggt actatacgaa ggattacaac ccggtggtca attaactaca
     1321 actaccgaag tcgacaattt tcccggatat cccgaaggtg ttaccggtcc gcaattaatg
     1381 gaagatttac gaaaacaagc tcaacgattt ggaaccgatg tgcgttcggg ctatgcaact
     1441 gctgttgatt tttcgggaac caataaaatt gtagaaattg atggtgaaaa gaaaatagaa
     1501 gcacaagttg taattatatc taccggtgct accgctaagt atcttggact tgaaagcgaa
     1561 gaaaaatata aaggtatggg cgtttcggca tgtgctactt gcgatggctt tttttatcga
     1621 aaacaagatg ttgccgtggt aggcggtggc gacactgctg ccgaagaagc tacatacttg
     1681 gcaggtttag caaacaaagt atatttaata attcgcaaag accacatgcg tgcaagtaaa
     1741 gccatgcaaa aacgagtaga aaatactccc aacatagaag tattgtatga acatgttaca
     1801 cttgaagtac ttggtgatgc aaacggggta aatggagtac gattactcaa aaaaggaacc
     1861 gaagaagtta ccattaatgt tacaggtttc tttttagcta taggacatac gcctaataca
     1921 gaaatattta aaaactttat agaacttgac gaggttggat atatcaaaac aaaaccgggt
     1981 tcaactcata ccaatattcc gggagtattt gcatgcggcg acgtacaaga cccacactat
     2041 cgtcaagcaa ttacagctgc cggatcgggt tgtatggctg ctttagatgc cgaacgatat
     2101 ttgggaagtc ttttgtaatt gttaattata aatggttaat ggttaattga atcttctatc
     2161 tgtaatgaaa atttatacaa aatcaggtga taaaggcaca tcgtcgttgc ttggtggtac
     2221 acgtgtagct aagtatcacg ataggcttga gtgttatggc actatagacg aattaaattc
     2281 gcatataggt gttgtacgtg acctttgtaa taataatgct gtatgtgaaa tattagctca
     2341 cattcagcac acattgttta ctataggttc atatttggcc aatgaaaaac ctcatgaacg
     2401 cacaacgccg attttatcaa cacagcacat tgaattactt gaaaaaacta ttgatacata
     2461 taccgaagaa ttacccaaac ttacacattt tattttacct gccggagata ttgtatcatc
     2521 gcatacacac gtatgcagaa ctgtatgcag aagagcagaa cgacttattg taaagttatc
     2581 ggaatctaca cacatagaag aaaatgttat aatatatatg aatcgtttat ctgatttttt
     2641 gtttgttttg gcccgaaagt ttttaaaaga tgttaacaaa caagaaataa catggaatcc
     2701 tctgttgtaa attagtattt ttttatatat tttcgcagaa attcactaaa aattggaaac
     2761 ctatgtattg gacattagaa cttgcttcaa aattagaaga tgctccatgg ccggcaacta
     2821 aagatgagtt aatagatttt gctattcgtt cgggtgcccc tcttgaagtt attgaaaact
     2881 tacaagaaat tgaagacgaa ggtgaagtat acgagagtat tgaagatata tggccggatt
     2941 atccgagtaa agacgatttc ttctttaatg aagatgagta ttaaaaaaat gcaaaaaggt
     3001 gttttattgt aaaacacctt tttttttata tattactatg gattaccttt ggcatatact
     3061 aaaagaactg cttacaacta ccttattaca aatttggata gtatttggta ttttctttat
     3121 tctaggttta ttactctatt ttttttctcg tagcacacgc aaggtatttg ttacatcagg
     3181 gtatcccaaa attgatatat ttattaccgg ctggattgga acacctgtgc atgaattagg
     3241 acatgcatta ttttgtgtgt tatttggaca tactatacaa gacatgaaac tctttaaacc
     3301 tgacaaagcc gatggaacac ttgggtacat aacacattct tacgataaga aaaatctata
     3361 tcatactgtt ggcaactttt ttataggctg tggccccata ttattaggct ctgcaatgtt
     3421 atttatactg ttttatcttg gatttcctct ttctcatgaa atagcaactg tatttaacac
     3481 atatggaaat atttacattt catacaatac tatcatagaa tcgttacaac aaatagttac
     3541 gttggtatgg cacatgtttt ctgtaattct tacaaaacaa cacatcaatt cctggcaatt
     3601 ttgggttatt gtatatattg cattttctat tgcttcacat atgcaactaa gcccacccga
     3661 tattaaaatt atgttgaaag gctttcttgt tatattgggt ttacttacag tagttaatat
     3721 agtagccgta gtatgggaat ttgatataaa tgcatggatt gtagttcatt tcaaaattgg
     3781 atatatatat ggtatgcttg tatttgccac atctatatcg gcagtatttt ttatagtatc
     3841 atatatttgc atggctatag tgtacggtat ttcaaaacat aaattattac ccctgtttgg
     3901 gtaactgaat atgaaacgta ctgcctacac ctatttgcga tgttacccaa atagttccat
     3961 tatttttttc aataaattct ttgcttacta taagccctaa tcctgaacct tgattttttt
     4021 ttcgtttata tgattgtgaa ggttttattg taaaaagagt ttgtatttct tcgggtgaaa
     4081 taccggctcc attatcagaa atagtaacag taatatacgt atgtgtttca actgcttcaa
     4141 aacaaacttt gccattatta ggtgtaaatt ttattgcatt gttacaaata tttctcattg
     4201 cagtaattag catttcttta tcaacaaata catacagatc ctttggtata gtattacata
     4261 attgtatttg tttttcctga gccgccaatg catacaattc aactacacgg tctgccaaca
     4321 agtataattg acacatctca ggattacatt gtatagaacg tgtctgactt cgcgaccaac
     4381 ttaataaatt ttcaagtaaa ttataaatta ccgttgccga ttgggtaata agagcaatat
     4441 aatttctttt ttctttttct tgaattgttt cgtattcctc atgcaataat tcagaaaatc
     4501 catgtaacga agtaaaagaa ttacgcaaat catgagcaac aatagaaaac aagtaatttt
     4561 tagtatcatt agcttgttgc aattctacat tttgttcttc tatatatttt ttttgttcta
     4621 taatagactg agtctgacgt tttacctctg cctcaagttt ttctttttgt gatttgagtg
     4681 tatatattcg aatataataa atgccataca atattgcagt aataaaaagt aacaacaaaa
     4741 ttttgaacca ccatgttttc caccacggcg gcaatatatg aacagacaat tgtattacct
     4801 tggcttcatc ttgattatta atccatgctt ttacaaaaag cacatacctg ccgggcggaa
     4861 tattggtaaa tgtaatttca cgtttgttcc ctatagtatt ccaatctcta tcgaatccct
     4921 caagtttata agaaaatacc accgcatcgg gggcattgta acaaattgca tcaaaactca
     4981 caccccaaat attttgtttg tacgaaatag ttaattcctt aagttgcgaa atatgtgtag
     5041 gataccaatc ggtatttggt gactgctttt gatacgaaat aaataaatca gaaaataaaa
     5101 attgtaaaat actatgtttt tgttgcatac tatttggata aaatgaatac aatccattca
     5161 tagtacccca atgagttcgt ccttgtgtat cttgatacac cgctcgtgat gtatagaaat
     5221 ttcctttaga ataattatca acataataaa tttcatattt ttttgtactc acatcataca
     5281 caatcaatcc gttattagtt gcaatccaat attgtttatt ggcactacag tgtagactca
     5341 aaacttctgt accttgcagc tcgggaattg tttttgacaa ttcttcaagt aattcacctt
     5401 ggtttgaata cacaaataaa cctctattgg ttcctataac tatatttcct gcaaaatctt
     5461 cggtaagtgt atgaacatac ccaggaaaaa aagacaattt atcattatca aaattcaata
     5521 caagcaaaga attattggca gtaattttat acaccgtacg caacgatgct acccacattg
     5581 ctccgtttct gtcacggatt atttgattta taaatttttg acgatctgtt ttaaacagcg
     5641 tatctaacgc tattattttt cgtttaagtt gccccgacgg tatatcaaat tcctgaacac
     5701 catttccgta agtacctacc cataatgtat ttgcatcata aaaacaaatt gtttttacca
     5761 aattatttgt aatttcagtt tgcgataaat tatagggagt aacacgcttt gtaatcatat
     5821 caattttcat tataccactt gcccacatac aagcccacac ttcattttca gttcgttttg
     5881 caaacgctaa tacggaattt gagggtatat agggtgtttg aatttctgat aaatacccgt
     5941 aatatggtga aaatatactt ataccacagc catcggtacc tatccacaaa tttccattac
     6001 catcttcggt aatagcactc acaaccttat gtgccagccc cacttccatt gatgtatgct
     6061 gcactttatt tttagacgga ttatacaaaa acagtccatt cccttgtgtt gctacccata
     6121 tagaattttc tttgtcacat agtattgcat ttacacttgc aatttttatc ccttctgttt
     6181 gataatcttc tattttataa aacaaattat ttgcatcata tacccacaaa ccattataat
     6241 ttgttccaat ccaaatatta tgagaagagt cgcgtacaat tgatgatata taattttgtt
     6301 ctaccgtgtt aaaatatgtt gattttacaa ttatgtgatt tgaaggttgt aattcatata
     6361 tacctcgcga agttcctatg tatatcaaat ttttattaat tttacaaaac gatggaattc
     6421 tgagtatccc taattcatct tgaattggaa acaccttgac tgttttttgc tgagcatcaa
     6481 catataacag cccactttta gctgttccaa accaaaaatt accggcatca tcttgtatca
     6541 tagaaattac atgtatctgt ttaaaaaccg gaaattcttg aaatgcaaag ttaaaagtac
     6601 ctgattgttc attatacagt aataccccat gacttgtaag agcatagagt ttattatgcg
     6661 attcgtaaaa tcttgaaata gatggatatt ctaaatgata ctcaaaatcc aatgatacat
     6721 tttggaactg atctttttct tgattataca tcattatagt tccattgtgt gaaccaaacc
     6781 atatacgacc tttagaatcg gtaaacacac aattaaaatc atttcgcaaa accgactgat
     6841 attgatgttc tgattttata aatgaatgaa acagtaaccc atcgaatctg tttattccat
     6901 aggaagttgc catccaaata tagcctgtag tatcttgaga gagagaaaaa ataaattcgt
     6961 ctgataaccc gtcatgacga gcataatttt gaaaattaca aaaaaattga gcgttcccac
     7021 tatttaaaga aactaaccaa aatacaacaa atataattat tctattcatg agtgagattt
     7081 tgctctttgt tacaaaaaca atattaatac aaacgaaatt cgctaacagg taaacaacct
     7141 ttcaaatata gttatttttt ttgttttcaa ccatagatta tacaatttct attttgttat
     7201 cagtgatatc tgtggcgaaa aataatttag caatagaata ctaacaataa aagtgtactt
     7261 ttgcaaaaaa taataatata tgattacagt ttcaaattta gccatacagt ttggtaaacg
     7321 cacactattt caagatgtta atcttaagtt tataccgggg aatatatatg gtgttatagg
     7381 agcaaatggt gcaggaaaat ctacattttt aaaaatactt tccggcgaaa ttgaatctac
     7441 tcgcggttcg gtaagcttag aacctaacga acgattatcg gttctcaaac aaaaccactt
     7501 tgaatatgac gaatgtacgg taattcaaac cgtacttatg ggttacaaag aattgtggaa
     7561 cataatgaat caaaaagatg cattatatgc aaaacccgat ttttctgacg aagacggctt
     7621 acttgctgcc gatttggaag aaaaatttgc cgaaatgaac gggtggaatg ccgaaagcga
     7681 tgctgccatg ttgttgagtg gattgggtat tgccgaatcg catcacaata aacaaatgaa
     7741 agaactcagc ggtaatgaaa aagtacgtgt aatgcttgct caagcactat ttggcactcc
     7801 cgataattta ttgctcgatg agccaacaaa cgaccttgat gtggaaacag taatgtggct
     7861 cgaaaatttt ctctcaaatt ttgataaaac cgtgttggtt gtttcgcacg accgtcactt
     7921 ccttgatgct gtgtgtacac acactgttga tatagattat ggcaaaattc aactatttgc
     7981 cggcaattat tcgttctggt acgaaagtag tcaattggct ctgcgacaac aacagcaaca
     8041 aaacaaaaaa gccgaagaaa aaaagaaaga attattggaa tttattgctc gctttagtgc
     8101 caatgttgca aaatcaaaac aaactaccag ccgtaaaaaa atgctcgaaa aattaaatgt
     8161 agaagaaatt ctaccttcaa cacgtaaata tccgggaatt atatttaaac cttcgcgtga
     8221 tgttggtaac aaaatattag aagttcacgg actatcagca aaaatggaag gcgatgtatt
     8281 gtttaaacaa gtagatttta cggtagaaaa agacgataaa attgtattcc tttctaaaga
     8341 cccacgtgct atgaccgcac tgtttgaaat tataagcggc aacaaaaaac ccgacggcgg
     8401 gcattttgat tggggcgtta ccgtaacatc ggcatattta ccactcgaca attcccaatt
     8461 ttttaatacc gatattagtt tacttgattg gcttgctcaa ttttctgatg atacacacga
     8521 aatatttttg cgcgggtatt taggtcgtat gttattctcg ggcgaagatg tatacaagaa
     8581 atcaagtgtg ctttcgggag gagaaaaaat gcgttgtatg atttcgcgta tgatgctcaa
     8641 agatgctaat gttcttatac ttgactcccc tactaatcat ttagatttgg aatctattca
     8701 agcatttaat aattcactta tgcaatttca aggcatagtg ctaatgtcat cacacgacca
     8761 tgaatttatt caaaccgtat gtaacagaat cattgaactt actccaaatg gaatcattga
     8821 caaacgtatg gattacgacg attatataat tgacgaaaaa gtaaaagaat tacgaaataa
     8881 attatataaa taagcttcta tgacattcga cgaattcaat attcatgaac aattactcga
     8941 agctatttcg tatatgaatt acgaaaaagc atcacctatt caggaattag ctattccacc
     9001 cgcattgttg ggccacgata tattggcgtg tgcacaaacc ggaaccggaa aaacagctac
     9061 atttgtaata ccaataattc atgatattgt aactaataaa ttagaaggaa ccactagtct
     9121 cattcttgtt cctacacgcg aattggctat acaaattcat caagaaattc aaggattttc
     9181 atactttaca gacactacca gcaaaccaat ttatgggggc gacaaaggtg ccgattggga
     9241 tgcacagaaa cgagctctta ccgaaggcac caatataatt attgcaactc ccggtcgttt
     9301 gcttgcgcat ttaaaattgg gatatgtaga ttttaaacaa atacaacatt ttgttctcga
     9361 tgaggctgac cgtatgctcg atatggggtt ttacgacgat atacaacaaa tattatcgta
     9421 tattccccaa caacgacaaa cattattttt tagcgcaact atgcctccgg caattcaaaa
     9481 acttgccgac aaaattttac aaaaccccaa agaaattaga atatcaattt caaaaccggc
     9541 cgaaggagta actcaaaaag catatttagc atatcctcac caaaaaaatg gggtaattca
     9601 gcatatttta caacaaaacc ccagttacga cagcatcatc atttttacct caactaaacg
     9661 aatggtacaa aaaattgtac acgatattgc acgcaaaaat cccggtatta cggtagaggg
     9721 tatttcttct gatttagaac aaaaagaaag agaagaagta ttactcaaat ttcggtcaaa
     9781 aaacacaaaa atattagtag ctaccgatgt tatgagcaga ggtatagaca ttaaaggaat
     9841 aaatttagta ataaattacg acgtaccggg cgaagcagta gactatgttc atcgtgtagg
     9901 acgaacagct cgtgctgact ctaaaggtga ggctattaca cttataagcg atgttgatat
     9961 gtataaattt gctcgtatag aagcattaat tgaaaaagaa atacctaaac ttcctatacc
    10021 tgctcattta ggtgaagctc cggtatggaa atcaaaacaa tcaaaatcat tcaaatcaaa
    10081 actacaagag aataaacagc aacataaatc acaaggagac ggaaactcat ctcaaaaaca
    10141 aaagaaaaaa cggttttggc gaccaaaaaa gaaaccaaat tcacaatcaa atgaagcgca
    10201 ataaaggttt ttggattact ataatcatac ttatagtaat tggcatagga atatatttgt
    10261 atttcaacta tattcaagcc caacccatac ctgctaacaa tacatatacc tcatacaaac
    10321 ccattcctga acttgaaata ccttgtggta cacaatatat taagcatagt cattatgcct
    10381 ttgaatataa agaagaacac gaacaatcaa tttgggtagc gtatatgctt acttcaaaaa
    10441 aaaatgcaca aacttacaag cgttcaaact cattttatcc cgacccattg gtttcaacag
    10501 aaactgccac aaacaaagac tattcaaaat caggctacga caaagggcat cttgccccgg
    10561 ctgctgatat gaattggtcg gaacaagcaa taaaagaatc attttatttc agcaatattt
    10621 cgccacaatt gcctgcattt aatcgtggaa tttggaaaac acttgaagaa caagtgcgta
    10681 cgtgggcaca aaaatttgac acattatata ttgttacagg ccctatagta gaacccggac
    10741 tacctactat aggtgaaaac aaagtaagca ttcccaacta tttttacaaa gcaatattag
    10801 tttatagcta ttcacgggca tttggaattg ggtttatact tccacatgca gcctcaaata
    10861 attcaatata ttcatttgtt gtacctatag atagtattga aagcaaaata catagagatt
    10921 tattttgcaa attaccggac actattgaag aaaaaattga atcatcgact accatttcag
    10981 attggaaatg atgatttttt atacaatagt atttctattg tatctattat tcaaatttga
    11041 tttaattcac atatttttga ttgtatattt gcaacatggg tactcaatct agtattacac
    11101 ttcttgaact tgcacaagct atagaacttg gtattcaaac agctttgcca aatagttatt
    11161 gggttgtggc agaaataagt gaattaacgg taaattcaaa aggtcattgt tttttagaat
    11221 taattgaaaa acctgaacat aatgaaacaa ttattgcaaa attacgagct actatttggt
    11281 catacaaatt tcaactcctt caatcatatt tttatgcaag tacaggtatt catctacaga
    11341 caggactcaa agtgttaata tgtgtaaaag ttgaatttca tgctgtatat ggattaagtg
    11401 tttcaattca tgatatagac cctgtttata ctattggcga agaagaaaaa cagcgtaacg
    11461 aaataattgc acgattacat gatgaaggta tttttaccat gaacaaagat cttaaattac
    11521 ctgtattccc ccaacgatta gcagtaattt catcagcaca ggctgcagga tatcaagact
    11581 ttgtaaaaca aattgcttca aacaaaaatt cattacgtgt ttctattact ttatttacag
    11641 cagccatgca aggaatagaa accgaacaaa gtataataaa agctctcaat agtatatatg
    11701 aacgcgaaca cgaatttgat gcagtaataa ttataagagg cggtggagca aaaacagact
    11761 tacgatggtt tgataactat actatagcat cacatattgc acaatttcca ctcccggtaa
    11821 ttactggaat agggcatgac aaagacattt caataaccga tatggttgct cacacttcgg
    11881 ttaaaacacc tacagctgca gccgaattta taatttcaat ggggaatgct cttgtatcaa
    11941 ctattgataa tttacacgca cgtctttctg atgcatgtaa tgaacgtata gcagaacaaa
    12001 aatttaccct acaacacatc ttacaagagt tatttcactc aaatacatta cgcacaacta
    12061 ttgcaaataa taaattaaat aactgtatac agaaaataaa atcggcaagt ttgtttacaa
    12121 ttcaaaagca aaagcatttt atagaacata taagttcaaa tataagccct gctatacatg
    12181 cacttacaca taagcaccaa caaaatctca tacattcagc atacaaatta caaattcatt
    12241 cacataattg tatattacac aacaaacaat ctttagagtt attacaaaca aaaatagaat
    12301 tacacaatcc gcaacgtata ctctcaaaag gatacagtat tacaaccaat gcacatggaa
    12361 caataataaa ctctattgat tcggtacaac ctaatgataa actatatacc tatataaaag
    12421 atggtattat tacaagcaca ataaataaaa aagacgctac taattaaacg aaattagttc
    12481 aaacggaaca tgaataaatt gtttgtattc taaaccggca tcacgcatat catcatcttc
    12541 ttccatataa tgccactgta tagtaacttt acaatctcct gtattttgaa ttttttctaa
    12601 taaatgaaaa atttccatca aaaattttga cgatgcagta ttataatact ccaaattaat
    12661 agaaacagta aataaagaac ctaaattact tgtgtttgat tcaatccatt gtatgatagg
    12721 cccataaaaa tcacgagcat tttcaggaaa cgatctgcct tcaatacgca atgtagcttc
    12781 atcgctacta taccatactt taggagtaga aggagtcttt ttaatttcta attctgtcat
    12841 actgtttttt ttacaaaaat acaattttta tttatatatt acatatacag cattttagtt
    12901 tttctgcctt aactaatatt tatattg
//
LOCUS       DAOTOW010000002        11192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_5490_length_11192_cov_3.557870, whole genome shotgun sequence.
ACCESSION   DAOTOW010000002 DAOTOW010000000
VERSION     DAOTOW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5490_length_11192_cov_3.557870"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="P5243_00065"
     CDS             <1..223
                     /locus_tag="P5243_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HRS17868.1"
                     /translation="VAVARALMNNPSIIFADEPSGNLDSKHKTELHELFFTLRNEFNQ
                     TFVIVTHDNSLAQLSDRVITMEDGNIVIN"
     gene            complement(220..1692)
                     /gene="cysS"
                     /locus_tag="P5243_00070"
     CDS             complement(220..1692)
                     /gene="cysS"
                     /locus_tag="P5243_00070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRS17869.1"
                     /translation="MMQKLIIYNTLRRKKEEFNPINPPHVGMYVCGPTVYGEAHLGHA
                     RPSVTFDVLFRYLQHIGYKVRYVRNITDVGHLENDADTGEDKIAKKARLEQLEPMEIV
                     QLYTLNYHDCMKALNVLPPSIEPRASGHIIEQQEYVQRILDSGFAYESMGSVYFDVEK
                     YNKQHNYGILSGRNIDDMLSNTRSLDGQDEKRAPLDFALWKKAKPEHIMQWNSKWSKG
                     FPGWHLECSAMGEKYLGKEFDIHGGGMDLMFPHHECEIAQSTAANGTSAVRYWIHNNM
                     ITINGQKMGKSLGNFITLQELFTGSHKILEQAYTPMTIRFFILQAHYRSTLDFSNEAL
                     QAAQKGMERLLQGYQTIQKLPVSAQSSIRVDELRAKCYDAISDDLNTPVLISHLFVAL
                     KDIQAIAAGAASITQSDKDALAELYRVFLFDILGLQSHDEQQSNNSELLHTAIEMLLQ
                     VRLEAKKNKDFAKSDEIRNTLTNLGVVIKDTKDGYEWSLS"
     gene            1842..2423
                     /locus_tag="P5243_00075"
     CDS             1842..2423
                     /locus_tag="P5243_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HRS17870.1"
                     /translation="MKLVFATNNKHKLQEIRAIAGKFFEIISLEELGCSDEIPETGET
                     LEDNAIQKATYIYEKYGHNCFADDTGLEIEALDWAPGVITARFAGEHCSPDDNIRKTL
                     ELMEGKTNRYAIFRTVIACIIDGEEYLFEGAVEGTIATQRMGEQGFGYDPIFIPAGYE
                     TSFAQMPMSIKNTISHRGRATAEFMKFLHEISL"
     gene            2536..4440
                     /locus_tag="P5243_00080"
     CDS             2536..4440
                     /locus_tag="P5243_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRS17871.1"
                     /translation="MSDSIKHECGIAFIRLLKPLEYYQAKYGTWKYGLNKLYLLMEKQ
                     HNRGQDGAGIAFCKLDAKPGTEYLTRIRSNKNSPIAKIFNNINTPYLEIQKTNPELLQ
                     DANWIKHNTPFAADIYLGHLRYGTFGGNDIANTHPVSRENNWKTRNLMLAGNFNLANM
                     DELFNKLLDLGQHPRGYTDIVTILEKIGHFLDRENQKLFTEYKTSGLNNVEISEAISQ
                     NLNVPNVLREASKYWDGGYVIAGIIGHGDSFITRDPAGIRPAFYFANDEIVVAASERP
                     VIQTVFNVPLEEVQELEPGHILVVKRNGTIVNEMIREPQERRACSFERIYFSRGSDID
                     IYKERKKLGEYVVPQILQSVHYDIENTVLSFIPNTAESAFYGMIKGMQDYIDTIKQTK
                     ILELQQQGTLTPEALAQILSLRPRSEKLAIKDVKLRTFITEDKSRDDLVGHVYDVTYG
                     IVKPTDNLVVIDDSIVRGTTLKKSIFKILNRLHPKKIIIVSSAPQIRYPDCYGIDMAK
                     LGDLCAFNAAIALLKETKQESIINAVYNKSAAQRHLPKEEIVNYVQEIYAPFTDEQIS
                     KKIAQMLTPEDVTAEIDIIFQSVANLHKACPNHKGDWYFTGNYPTPGGNRAVNNAFLN
                     YIEGRNERAY"
     gene            complement(4437..8543)
                     /locus_tag="P5243_00085"
     CDS             complement(4437..8543)
                     /locus_tag="P5243_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS17872.1"
                     /translation="MIPEKVIVLTQNSHIVDRISQCGILEPIRHIRTTHELPAYSENP
                     FLLLVDIAMSVYAKDLHFEGSKKILIVPSERELGIDLFSSFDDYISLSATNQILALRL
                     NTYVGTIFQSRNLVNNQNKYKQLVEQSGDTVILLDKYGIMCEVNSHVCMVSGFSDTEL
                     LQHHIRKIFPDTNFIEKLHPAIPSGSLVETMLHTKFDTLKPKEIRISKIILEQELYFL
                     VIARDISERLKTLKKIEDSEERFRTVIKHSPNGMIIFKDDTISFINLVGLKILGGTYE
                     DFIDQSFFRLVSPAYRADLLQFIKKSTKRDEPISKDVTFIDLQNKEVSVNLNCVPIDI
                     QGEIHYFIIFQDLTELKRTQYDLETKDLRLREIQKIAKLGHYEINIIKNNVYWSDEVY
                     KIYGVSQRTFSPTYAKTLEFVHPDDRETVRKAYENSLITREPYEIVHRIILPNGTIKY
                     LNETCSTMYDEHGVPVRSLGTVMDITHTIRTEKALQQTEEKFRLLFENLDEPFLICKP
                     SYDETGKVFDFIITELNPACKKLFRGNYLDVKEHSIREIFYEPDIWIEKYTYAFEHSE
                     TISFRKYSTDLHKYLDIVLFPVMGKKQIAGIYSDVTEKVYSENQLKQLTARLQKIQEY
                     AKIGFYDVHIVTHKSMWSDVMIQIFEYSKKDKPSFDLYLQRIHPDDRENVRQAYSDSI
                     KKHKTYVTLEYRLLFPDGRIKHIYTEFYNTFEGKTCLHTEGWLQDITDLTIATSALRE
                     SEEKLRMVTSGTQLGLWEWDIFNKEFLIDETGSQMLGYKPQELWGDDTIYLDLIHKED
                     KEVVEKKLIKFWQRTNQLFNAEFRVLMKNGRYKWVSCVGVSSEYRMGKVSKMIGFNQD
                     ISLRKKAEEDLRDSENKFRRIFEIENDSLFLADSKTGKILETNQAAVKLYGYSRKELL
                     SRNFQDLSVNPVNIMTLIHRQKTRVDSEIAVSKHGVHCPIEISFAYFTWKGKDVFLAA
                     VRDVSERRRVEQELRSAKEKAEESDRLKSAFLANMSHEIRTPLNAIVGFSRLLARKNY
                     DQEKRKIFIDDIQSNSNQLLTIINDILDISKIESGQFILNPTQVCINKLLQEVYDTMQ
                     LQIKDKDLVLFCEKPLPDSDVTITIDEVRLKQILTNLLNNAIKFTEQGYIHFGYKAQK
                     SKQLLFFVRDTGIGIAPEKHAIIFEHFRQEDDTTTRKYGGTGLGLSISKKLIELMGGT
                     IWVESEKNKGAQFFVTVPLIQSQTLDHVTNVTHIETVAHASFNGETILVVDDHTSSYI
                     FISEMFENQNVRVIQATSGEQAIELCKQFPTIQLVLMDIQMSGVSGVDAMKQIKQLFP
                     AIPIVAQTAFAQKGDRERFMQQGFDDYITKPLDERDLQEIFKRFLSSN"
     gene            complement(8559..9404)
                     /locus_tag="P5243_00090"
     CDS             complement(8559..9404)
                     /locus_tag="P5243_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17873.1"
                     /translation="MKKNILLLTALLLLSIYSCRKTENPTYFDVITTSKDEALIQSLF
                     DDAQNESEKQHIASQDDALKSELTDSVIVTVDGGANGFEDTAWTITIDYGDGYTNKYG
                     WVKRGKINIAVTGFYRMKGSKRVMTFDNYFVNNQKVEGQHVVENIGFDSTTLAYNFKV
                     TVTNGKLTLADGKIITWESERNRAWTKGYDTPLSILDDEYSITGEAHGVNSEGKAFTK
                     TIIQPLVIRPTCQFIQKGIIDISIEGEHMANVDYEYGYVNDQTCDKQAQVVTKNRTFN
                     IEIQR"
     gene            9513..10031
                     /gene="frr"
                     /locus_tag="P5243_00095"
     CDS             9513..10031
                     /gene="frr"
                     /locus_tag="P5243_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRS17874.1"
                     /translation="MNSPLHRLDIELSKLRAGKASPAMLETVKVDYYGTLTPLSQVAN
                     INTPDPRTLIVQPWEKKMIDPIEKAIMAANLGLTPVNDGSIIRISIPVLTEERRKELV
                     KQVKTEAETAKVSVRNIRRDANEMLKKLQKDGLPEDVAKDKEIEIQKITDATIAKIDD
                     KVALKEADIMKV"
     gene            10163..>11192
                     /locus_tag="P5243_00100"
     CDS             10163..>11192
                     /locus_tag="P5243_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17875.1"
                     /translation="MKTFNYKPLVQGIAIVALFFISIMIYFSPIFTENKQLSSHDVMQ
                     YKGMSKEIIDFNEKTGEQTLWTNSMFSGMPSYLIHTTYSNFTSYIHRIFNTSHETPYM
                     IAFMYFLCFFLALLLLDFSMLLSMLGALFYGFSSYFFIIIEAGHITKAIALGYMPLVI
                     AGVVAAYRSKLLLGTVVFSLFLSLQILINHLQITYYTMLILLVFVGFQLYESIKNKEF
                     VSQFIKPSAFLLVGALLAIASNFGSLYMTFDYGKDSMRGKSELTHDKENKTKGLDKDY
                     ATAWSYGIDETCNLFIPNFKGGSSSGSLTEDSETYKTLKQNNVPRANQVIKNLPLYWG
                     TQSSTSGPV"
BASE COUNT         3770 a         1944 c         1724 g         3754 t
ORIGIN      
        1 tgtagctgtg gcacgtgcac tcatgaacaa tccatctata atatttgccg acgaaccttc
       61 gggaaattta gacagtaaac acaaaaccga attgcatgaa ttgtttttta cccttcgcaa
      121 cgaatttaat caaacatttg tgatagtaac ccacgacaat tcattagctc aattgtctga
      181 cagggttata accatggaag acggtaatat tgtaataaac taactgagag accactcata
      241 cccgtctttt gtgtctttta taacaacacc aagattggta agtgtatttc taatttcatc
      301 tgattttgca aaatccttat tttttttagc ctctaatcga acttgcaaaa gcatttcaat
      361 agctgtgtgt aacaactccg aattgttaga ctgttgttcg tcatgtgatt gtaaccctaa
      421 aatatcgaac aaaaacacac gatacaattc tgccaaagca tctttatcag attgtgtaat
      481 tgaagctgct cctgccgcta tagcttgaat atctttaaga gcaacaaata aatgtgatat
      541 aagaacagga gtatttaaat catctgaaat agcatcgtaa catttagccc tcagttcatc
      601 aacacgtata gaactttgtg ccgaaacggg taatttttgt atcgtttgat atccttgtaa
      661 taaacgctcc attccttttt gagcagcctg caatgcctcg tttgaaaaat ctaacgtact
      721 gcgatagtga gcttgcaata taaaaaagcg aatagtcata ggagtatatg cttgctcaag
      781 aattttatga ctacctgtaa acaactcttg taaagtaata aaatttccaa gagatttacc
      841 cattttctgt ccatttatgg taatcatatt attgtgaatc caatagcgaa cggcagaagt
      901 tccgttagct gcggtagatt gagcaatttc acattcatga tgcgggaaca taagatccat
      961 accacctcca tgaatatcaa attctttacc cagatatttt tcgcccattg cagaacattc
     1021 caaatgccaa cccggaaagc ctttactcca tttcgaattc cactgcatta tatgttccgg
     1081 ttttgctttt ttccacaacg caaaatcaag tggagcacgt ttttcatctt gaccgtcaag
     1141 acttctggta ttagaaagca tatcgtctat atttctaccc gaaagtatac cgtaattatg
     1201 ttgtttatta tatttttcaa catcaaaata taccgatccc atagactcgt aggcaaaacc
     1261 gctatctaaa attcgctgaa catactcttg ttgttctatg atatgtcccg aagcgcgagg
     1321 ttctatactt ggaggcaata cattcagagc cttcatgcaa tcatgataat ttaaagtata
     1381 caattgcact atttccatag gctctaattg ttctaatcga gcttttttag caattttatc
     1441 ttcaccggta tctgcatcat tttctaaatg ccccacatcg gtaatattgc gaacatatct
     1501 tactttgtat cctatatgct gtaaataccg aaacaataca tcaaacgtaa ccgatggacg
     1561 agcatgtcct aaatgagctt cgccatatac agttggtcca catacataca tacctacgtg
     1621 aggtggatta attggattaa attcttcttt ttttctccgt aatgtattgt aaattattaa
     1681 tttttgcatt atttcaatag tttttgtttt gaggctcaaa agtaattgat attgattgaa
     1741 tttgcaaaaa agattaccga gtttttatac aagtaataat tccgttgttt atatggaata
     1801 aaaacaatat gtatatttgc ctaaaataga gaaataattg tatgaaatta gtatttgcaa
     1861 caaacaataa acacaaattg caagaaatac gtgcaatagc aggaaaattt tttgaaataa
     1921 taagtcttga agaattagga tgttccgatg aaatccctga aaccggagaa acattagaag
     1981 acaatgccat acaaaaggca acctatatat acgaaaaata tgggcacaat tgttttgctg
     2041 atgatacagg acttgaaata gaagcacttg attgggctcc gggtgttatt acagctcgtt
     2101 ttgccggaga acattgcagt cccgacgaca atatacgcaa aaccttggaa cttatggaag
     2161 gtaaaactaa tcgttatgca atttttagaa cagttatagc gtgcataatt gatggcgaag
     2221 aatatttgtt tgaaggtgca gtagaaggca ccattgcaac ccaacgaatg ggagaacaag
     2281 gatttggata tgacccaata tttatacctg caggctacga aacatcgttt gcacaaatgc
     2341 ctatgagtat aaaaaataca attagtcaca gaggtagagc aacagctgaa tttatgaaat
     2401 ttttacatga aatttcatta taatatcctc aacaaataat tgtttttttg ttaaaaaata
     2461 gtaaaaatac ttggggtgct cgattaaaaa aaagtttaca tttgccttgt atatgataca
     2521 aattttaatt ctacaatgag cgattctatt aaacatgaat gcggtatagc atttatccga
     2581 ttactcaaac cactcgaata ttatcaagca aagtatggaa catggaaata tggtttaaac
     2641 aaactgtatt tacttatgga aaaacaacat aaccgaggac aagatggtgc aggtattgca
     2701 ttttgcaaac ttgatgctaa acccggaact gagtatttaa ctcgtattcg ctccaataaa
     2761 aattcaccca ttgccaaaat ctttaacaat ataaacaccc catatttaga aattcaaaaa
     2821 acgaatcccg aattattaca agatgctaat tggataaaac acaatactcc ttttgctgca
     2881 gatatctatt taggacattt acgatatggc acatttggcg ggaatgatat tgcaaataca
     2941 caccccgtta gtagagaaaa caattggaaa acacgtaatc taatgttggc aggtaatttc
     3001 aatttagcca atatggatga attgttcaat aaattacttg atttaggaca acatcctcga
     3061 ggatatactg atattgtaac cattttagaa aaaattggtc actttttaga tagagaaaac
     3121 caaaaattat ttaccgaata caaaacatca ggattaaata atgttgaaat ttctgaagct
     3181 atttcacaaa atttgaatgt accaaatgta ttacgagaag cttcaaaata ttgggatggc
     3241 ggatatgtaa ttgcaggcat tattggtcac ggtgattcat ttataactcg cgacccggcc
     3301 ggtatacgtc ctgcatttta ttttgcaaat gatgaaattg tagttgcagc ttctgaacga
     3361 ccggtgattc aaacagtatt taatgttcca ttagaagaag tacaagaatt agaacccgga
     3421 catatattgg ttgtgaaacg caacggaacc attgtaaatg aaatgattcg agaacctcaa
     3481 gaacgaagag cttgttcgtt tgaacggata tatttttcga gaggtagtga tatagatata
     3541 tataaagaac ggaaaaaatt aggtgaatat gtagtaccgc aaattttaca atcggtacat
     3601 tatgatatag aaaatactgt attgtctttt attccaaata ctgctgaatc agcattttac
     3661 ggaatgatta aaggaatgca agactacata gatactatta aacaaactaa aatattagaa
     3721 ttacaacaac aaggtacact tactcccgaa gcacttgccc aaatattatc attacgtcca
     3781 cgcagcgaaa aattagcaat taaagatgta aaacttcgaa catttataac cgaagacaaa
     3841 agtcgtgatg acttagtggg acacgtatat gatgtaacct atggtattgt taaacctacc
     3901 gataatttgg ttgtaataga cgattctatt gttcgtggta caacattgaa aaaaagtatt
     3961 tttaaaatac taaaccgact ccatcctaaa aaaattatta tagtttcatc ggctcctcaa
     4021 atacgctacc ccgattgtta tggaattgat atggcaaaat taggtgattt atgtgcattt
     4081 aatgctgcaa tagcactcct taaagaaaca aaacaagaat ctattataaa tgcggtgtac
     4141 aataaatcgg cagctcagcg acatttacca aaagaagaaa ttgtaaacta tgttcaagaa
     4201 atatatgctc cgtttaccga cgaacaaata agcaaaaaaa ttgctcaaat gcttacaccg
     4261 gaagatgtaa ctgccgaaat tgacattatt tttcaatcgg tggcaaattt gcacaaagca
     4321 tgtccaaatc acaaagggga ttggtacttt acaggaaatt atcctacacc cggtggtaat
     4381 agagcagtta ataatgcatt tttaaactac atagaaggca gaaacgaacg agcgtattaa
     4441 tttgaactca aaaaacgttt aaaaatttct tgcaaatctc gttcatcaag aggttttgta
     4501 atataatcgt caaatccttg ttgcataaac cgttctctgt cacctttttg agcaaatgca
     4561 gtttgagcaa caataggtat tgcaggaaat aattgtttga tttgtttcat agcatcaacc
     4621 cccgataccc cgctcatttg tatatccata agtactaatt gtatcgttgg aaattgctta
     4681 cataattcta ttgcttgttc tccactagtt gcctgaataa ctcttacatt ttgattttca
     4741 aacatctctg aaataaaaat atacgagctt gtatgatcat caacaactaa tattgtttcg
     4801 ccattgaatg aagcatgtgc cacagtttct atatgtgtta cgttagtaac atggtctaat
     4861 gtttgcgatt ggattaatgg aactgttaca aaaaattgtg cccctttatt tttttctgat
     4921 tctacccaaa tagtgccccc cataagctct atcaactttt ttgaaattga caatcctaat
     4981 cctgtaccac catatttacg agttgtagta tcatcttctt gtctgaagtg ctcaaaaata
     5041 attgcatgtt tttcaggagc tatacctatt cctgtatcac gcacaaaaaa cagtaattgc
     5101 tttgattttt gtgctttata cccgaaatga atataaccct gttcggtaaa tttaattgca
     5161 ttattgagca aatttgttaa aatttgtttt aagcgcactt catctatagt aattgtaaca
     5221 tcgctatcgg gcaatggttt ttcgcaaaac aacaccaaat ctttatcttt aatttgaagt
     5281 tgcatagtat catatacttc ttgcaataat ttatttatac atacttgtgt aggattcaga
     5341 ataaattgac cgctttcaat ttttgatata tcaagaatat cattaattat agttagtaat
     5401 tgatttgaat ttgattgtat gtcatcaata aaaatttttc gtttttcttg gtcataattt
     5461 ttacgagcaa gtaaacgcga aaaaccaaca attgcattga gtggcgtacg aatttcgtga
     5521 ctcatatttg ccaaaaatgc agattttaat ctatcgcttt cttctgcttt ttctttagcc
     5581 gaacgaagtt cttgttctac tctcctgcgt tcagaaacat cacgcaccgc agccaaaaac
     5641 acatctttac ctttccatgt aaaataggca aaactaattt caataggaca atgaacacca
     5701 tgtttgctta ctgcaatttc tgaatccaca cgagtttttt gtctatgaat aagagtcatt
     5761 atatttacag gattcaccga taaatcttga aaatttctgc ttagcaattc ttttcgtgaa
     5821 tatccgtata atttcactgc tgcttgattt gtttctaata ttttaccagt tttactatca
     5881 gccaaaaaca aactatcatt ttcgatttca aatattctac gaaatttatt ttcactatcg
     5941 cgtaagtctt cttcggcttt tttacgaaga gatatatctt gattgaatcc aatcattttg
     6001 cttactttcc ccattctgta ttctgacgaa acgccaacac acgacaccca tttatatcgt
     6061 ccgtttttca taagtactct aaattctgca ttaaacaatt gatttgtcct ttgccaaaat
     6121 ttaatgagtt tcttttctac cacttcttta tcttctttat gaattaaatc aagataaata
     6181 gtatcgtcac cccataattc ttgaggtttg tatcccaaca tttgcgaccc ggtttcgtca
     6241 attaaaaact ctttgttaaa aatatcccat tcccatagtc ctaattgggt accagaagtt
     6301 accatacgca atttttcttc actctcgcgc aacgcagaag tagcaatggt aaggtctgta
     6361 atatcttgta gccatccttc ggtgtgcaaa catgttttcc cttcaaacgt attataaaat
     6421 tctgtgtaaa tatgttttat ccttccatca gggaaaagca atctatattc aagagttaca
     6481 tatgttttat gtttttttat actgtcagaa tatgcttgtc gcacattttc tctgtcatca
     6541 ggatgtattc gctgtaaata caaatcgaat gaaggtttat cttttttgct gtattcaaaa
     6601 atttgaatca ttacatcact ccacattgat ttatgagtta ctatatgaac atcataaaat
     6661 ccaatttttg catattcttg aattttctgc aaacgagcag taagctgttt taactgattt
     6721 tcggaatata ccttctctgt aacatcggaa taaattcctg caatctgctt cttacccatt
     6781 accgggaaca atacaatgtc taaatattta tgtaaatcgg tactatattt tctaaacgaa
     6841 atagtttcgg aatgttcaaa tgcatatgta tacttttcaa tccaaatatc gggttcataa
     6901 aaaatttcac gaatactatg ctctttcaca tcaagataat tgcctctaaa taatttctta
     6961 caagccggat ttaattcagt aattataaaa tcaaaaactt ttcctgtttc atcataactt
     7021 ggtttacaaa tcaaaaacgg ttcatctaaa ttttcaaaaa gtaatcgaaa tttttcttct
     7081 gtttgttgaa gagctttttc ggttctaata gtatgagtaa tatccattac cgtacctaat
     7141 gaacgcacag gaacaccatg ttcatcgtac atggtactac atgtttcatt caaatatttt
     7201 atagttccgt tcggtaatat tatgcgatgt actatttcat atggttcacg tgttataagc
     7261 gaattttcat atgctttgcg taccgtttct ctatcgtcgg ggtgaacaaa ttcgagagtt
     7321 ttggcatatg taggagaaaa agtacgttgt gaaacaccat aaattttata cacctcatca
     7381 gaccaataca cattattttt gataatattt atttcataat gacccaattt tgcaattttt
     7441 tgaatttctc gtaatcgtaa atctttggtt tccaaatcat attgagtacg tttaagttcg
     7501 gttaaatcct gaaaaataat gaaataatga atttctccct gtatatctat aggtacacaa
     7561 ttaagattta ccgaaacttc tttgttttgt aaatcaataa atgttacatc tttacttatt
     7621 ggttcatcac gttttgttga ttttttaata aattgaagta aatctgctct atatgccgga
     7681 gacacaagtc taaaaaacga ttggtcaata aaatcttcat atgtgcctcc taatattttt
     7741 aaacccacca aatttataaa tgaaatggta tcatctttaa aaataatcat tccattggga
     7801 gaatgcttga taaccgttct gaaacgttcc tcgctatctt caattttttt aagcgttttt
     7861 aatcgttcac ttatatctcg ggcaattact aaaaaataaa gttcttgttc caaaataatt
     7921 tttgaaattc taatttcttt tggttttaaa gtgtcaaatt tagtgtgcaa cattgtttca
     7981 accaaagaac ccgatggaat ggcaggatgt agtttttcta taaaatttgt atcaggaaaa
     8041 attttacgaa tatgatgttg taataattca gtatcagaaa aacccgaaac catacataca
     8101 tgcgaattta cctcgcacat tattccgtat ttatcaagca atattaccgt atcacccgat
     8161 tgttcaacaa gttgtttata tttattttga ttattaacta agtttcgaga ctgaaaaata
     8221 gttccaacat aggtattaag ccgcaatgca agtatttgat tggtagcaga aagtgaaata
     8281 taatcatcaa acgatgaaaa caaatctatt cctaattcgc gttctgatgg tacaatgaga
     8341 atttttttag aaccttcaaa atgtaaatct tttgcatata cagacattgc tatatctacc
     8401 aaaagcaaaa aaggattttc ggaatatgca ggcaactcgt gcgtagttct aatatgacga
     8461 atcggttcta atataccaca ttgtgagata cgatctacta tatgtgaatt ttgtgttaat
     8521 actataactt tttcaggtat cataccttac atttcctatt acctttgtat ttctatgtta
     8581 aatgttctat tttttgtaac aacttgtgct tgtttatcac atgtttgatc atttacatat
     8641 ccatattcat aatcaacatt agccatatgt tcaccttcta tagaaatatc tataatacct
     8701 ttttgaataa attggcatgt tggacgaatt acaagcggtt gtattatagt ttttgtaaaa
     8761 gctttacctt ctgaatttac cccatgagcc tcgcctgtaa tactgtattc atcatcaagt
     8821 attgaaagag gagtgtcata ccctttagtc caagctctgt ttcgttccga ttcccacgtt
     8881 attatttttc catcggctaa ggtaagttta ccattggtaa ctgtaacttt aaaattatat
     8941 gccaaggttg ttgaatcaaa tccaatattt tctacaacat gttgaccttc cactttttga
     9001 ttatttacaa aataattatc aaaagtcatt acacgttttg aacctttcat tctatagaat
     9061 ccggtaactg caatattgat ttttcctcgt tttacccacc catatttatt tgtatacccg
     9121 tcaccataat ctatggtaat agtccatgca gtatcttcaa atccatttgc tccaccatct
     9181 acagttacta taacactatc ggttaattct gatttaagag catcgtcttg actggcaata
     9241 tgttgttttt ctgattcatt ttgagcatcg tcaaataaac tttgaataag agcttcatct
     9301 tttgatgtag taataacatc aaaatacgta gggttttcag tttttctgca agaatatata
     9361 cttagcaaaa gtagggctgt gagtaataga atattttttt tcataattca gaattttatg
     9421 tatttttgta cacatcaaat atacattttt attacaaaat aaaaaacatg gaagaagaag
     9481 tagaattaat tttggaagat acaagagaac gcatgaattc tccattgcac cgattggata
     9541 tagaattgtc taaattacgc gcaggaaaag catcgcctgc aatgttggaa actgtaaaag
     9601 ttgattatta tggtacactt acaccgttaa gtcaagtagc caatataaac actcccgacc
     9661 ctcgtactct tatagtacaa ccatgggaga aaaaaatgat agaccctata gaaaaagcaa
     9721 taatggctgc aaatttaggt ttaacaccgg taaatgatgg ttctattatt cgaatttcaa
     9781 ttcctgtttt aactgaagaa cgccgtaaag aacttgtaaa acaagtaaaa actgaagccg
     9841 aaacagcaaa agtaagtgta cgcaacatac gacgtgatgc caacgaaatg ctcaaaaaac
     9901 tgcaaaaaga cggattgccg gaagatgttg caaaagacaa agaaattgaa attcaaaaaa
     9961 taactgatgc tacaattgca aaaattgatg ataaggtagc attaaaagaa gcagatataa
    10021 tgaaagtata atgacacgaa atagcacaca aaaaagcctg aaatagtact attttaggct
    10081 ttttttgtaa aaaaacaata tgaatacgtt ttttctgata caagcaaaaa cataatacaa
    10141 gtaacaaata aaaaacaacc aaatgaaaac atttaactac aaaccccttg ttcaaggcat
    10201 tgcaatagtt gcattgtttt ttatatcaat catgatatat ttttctccca tttttaccga
    10261 aaataaacaa ttaagttctc acgacgtaat gcaatacaaa ggtatgtcga aagaaataat
    10321 tgattttaac gaaaaaaccg gagaacaaac attatggaca aatagtatgt ttagcggtat
    10381 gccaagttat ttaattcata ccacttacag taattttacc agttatattc atcgtatttt
    10441 taataccagc catgaaactc catacatgat agcatttatg tacttcctct gtttttttct
    10501 tgcattattg ctgcttgatt tttcaatgct gcttagcatg ttaggagctc ttttttatgg
    10561 attttcttcg tattttttta taataatcga agccggccat attactaagg ctattgcttt
    10621 aggatatatg ccattggtaa tagcaggtgt agtagctgca tatcggtcaa aattattatt
    10681 aggtaccgta gtatttagtt tgtttttgtc attacaaatt ttaataaatc acttacaaat
    10741 tacgtattat acaatgctta tattgctggt atttgttggt tttcaattat atgaatctat
    10801 taaaaacaaa gaatttgttt cacaatttat aaaaccttca gcttttttac ttgtaggagc
    10861 attgctagct atagcgtcta attttggaag tttgtatatg accttcgatt acggcaaaga
    10921 ttctatgcgt ggaaaatcgg aattaactca cgataaagaa aataaaacaa aaggtcttga
    10981 taaagattat gctacagcat ggagctatgg tattgacgaa acctgtaatt tatttattcc
    11041 aaatttcaag ggcggttcat caagcggttc acttaccgaa gattctgaaa catataaaac
    11101 attaaaacaa aataatgttc cgcgagcaaa tcaggtaatt aaaaatctac ccttatattg
    11161 gggaacacaa agttcaacat cgggaccggt tt
//
LOCUS       DAOTOW010000003        10727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_5774_length_10727_cov_3.438718, whole genome shotgun sequence.
ACCESSION   DAOTOW010000003 DAOTOW010000000
VERSION     DAOTOW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5774_length_10727_cov_3.438718"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="P5243_00105"
     CDS             <1..381
                     /locus_tag="P5243_00105"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS17876.1"
                     /translation="AVEENERIKVQEFESEKTNEIFQVIQIAGDKMPIGIYSEQKSFE
                     SVRFQLIPNDSVYVFSDGYQDQFGGEKNKKFMVKRLKQLLVNINAESMEKQREILDKE
                     LVAWIKQGNTEQVDDILVIGFTVH"
     gene            400..2853
                     /locus_tag="P5243_00110"
     CDS             400..2853
                     /locus_tag="P5243_00110"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HRS17877.1"
                     /translation="MFAYSLSDIAHITNSFCKGNLQSIITSIYIDSRETHSNSGSMFM
                     AIPGKKFNGHSFIQKLYEEGIRNFWVQNGESYPEFDDAQYIISHNVIDAFQLLMKHYR
                     LQFSIPIVGITGSNGKTIVKEWLYYVCRHSMSVVRSPRSYNSQIGVPLSLLLLNSNYT
                     LGIFEAGISQPHEMQKLAAMLQPTIGIFTNIGDAHQEHFIDIEHKVTEKMKLFTHVSQ
                     LIYCKDYELIDKHAQMLLPNTKLYAWSFIDTSSSIFVQYSTQKHATHISVNYYSTVYS
                     FEIPYHDRASIENAVHCCITALVLGIETQYIQYFSDLPQVAMRLEQKSGKHNCTIIND
                     SYNSDLQSLTVALQFLSQQNQHPQKTLILSDLMQITADKKELYSHISSLIVTYGISKC
                     IGIGKDVQIYLKPKIQKGIFFNSTAEFLDAYTTLVFNNEAILLKGAREFEFEKIAKKL
                     EFQTHQTVLEINLNAIIHNLQYFRTYLQVHTKVMVMVKAFSYGSGSYEIANILQNYKV
                     DYLAVAFADEGIELRNAGITLPIVVMNPETGMMQQIFEHGLEPSIHNSTVLEEFAEYC
                     LAHSDRIFPYHIKIDTGMARYGFSPDEILSLIERVHAIPNGTIISVFSHLVGTDDSQF
                     DAFTEQQIATFVRSSSLICSQFSYPIMRHILNSAGIERFSKYQFDMVRLGIGLYGISA
                     QHEHNCMHISTLKTFISEIRSMPAGTTVGYSRKGTISRQSKIGVLPIGYADGLNRRLS
                     NGVGTVLVNNIEVPIIGNICMDATMIDITDVDAEIGDEVVLFGPRLPITVMAQRLGTI
                     PYEIITSISRRVKRLYYYE"
     gene            complement(2861..3280)
                     /locus_tag="P5243_00115"
     CDS             complement(2861..3280)
                     /locus_tag="P5243_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005680307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRS17878.1"
                     /translation="MWIQKKIQLTARSRGFHLITHSILDHLPEIQTFSVGILHLFIQH
                     SSASLTINENADPDVRTDFETFFNTIIPDTNRYFIHTHEGADDMPAHIKASVLGCSVS
                     IPITQGKLALGTWQGIYLCEHRNYGGSRKIVATIWGE"
     gene            complement(3284..4498)
                     /locus_tag="P5243_00120"
     CDS             complement(3284..4498)
                     /locus_tag="P5243_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRS17879.1"
                     /translation="MGNLGTQFSFGRGALLPQEEMLEVNTQRQNITIGLLAETDVNEH
                     RIALTPQAVKLLVEAGNKVLFERNAADRAKFTNQDYSEVGAIICDTKPEVYEADVLIK
                     VAPFNSQEISLLREKQTILSSFLINESALQQVHALTQKRINAIGFDYIRDIEGNYPVV
                     RSMDEIAGTMSILVAAEYLSNSNVGKGVLLGGITGISPAEVVILGANTAAEFAARAAI
                     GMGAHVKIFDSYLQRLISIQQALGQRVFTSNYHSGVLQKALKSAEVVIGTIQLVEDGQ
                     NFYVSEEMIKSMKPGSVIVDLSIDQGGCFETSACTTHKNPIFTKYGVTHYCVPNITSR
                     AARTSSIALSNIVIPILLKMSEMGGVKNFLKHDFAVRQGVYMYNGILTNSYIANKYGL
                     PYKNIDLLMAAF"
     gene            4698..6740
                     /locus_tag="P5243_00125"
     CDS             4698..6740
                     /locus_tag="P5243_00125"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRS17880.1"
                     /translation="MTHKNIVDRSADNLRILSMAMVEKSKSGHPGGAMGGVDFVNILY
                     SEFLQYDPTDFTWINRDRFFLDPGHMSPMLYSILHMVGTISIDEIKSFRQWGSVCAGH
                     PEVDHARGIENTSGPLGQGHTMAVGAAIAEKFLTQRFGAWMKHKIYAYISDGGIQEEI
                     SQGAGRIAGHLGLNNLVMFFDSNDIQLSTETKDVTSEDTAKKYEAWGWQTITINGNDQ
                     HEIRKALQLAQGDLDKPLLIIGKTTMGKGLVDEQGNSFERKTSTHGQPVSKAGASVEK
                     TILNLGGNPADPFAVFDDVAAYWQQIKQQKIAQAQAKKQLQTAWEKQNPELAQKLRDF
                     YAGKLPEIDYSKIEQKANIATRVASKTVLEYFAEHIENMIVSSADLSNSDNTDGYLKK
                     TKALKKDDFSGKFLESGVAELTMAAIMNGMVLHGGVIAACGTFFVFSEYMKPAMRMSA
                     LMELPVKYIWTHDAFRVGEDGPTHQPIEQEAQVRLMEHVYNHSGKRSFVILRPADAVE
                     ASVCWKMAMEINNRPVGLIFSRQVIKDIPAKQGSTRYTDALQAYKGAYIVNEPQREPD
                     LIMVANGSEVSTILEGAHILEEQYGLCVQVVSAPSEGLFLDQDEAYRNKVLPQHKPIL
                     GFTAGLPINLRGLVGPNGKVWGLNHFGYSAPFEVLDEKFGYTKENVVKQAVEYLGL"
     gene            7043..8305
                     /locus_tag="P5243_00130"
     CDS             7043..8305
                     /locus_tag="P5243_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17881.1"
                     /translation="MKILSKQIAISIIMMYALVSCSQTKSLHNYKAIETICKDADIIE
                     VEKKIDYTEVEFTCKGINYEVGIDLQGNIIYTEKAVSQNEIPFENIQKKIIKKYPDWS
                     IDEFSIVDLPDTSFIKVELLRNGIEENAFFTLDGKWFKPKNYVGKDTWNIHDLSETVY
                     YSASAYNYLNPTTIIDLPETLREISGIAVVNNTTLLCVQDELGAVFSLDLQKQEITNM
                     FRFTDIGDFEDIAYNNDTAYVLRSDGTIFSFAYTNFTGNIQQKIVQASSLNIEGLCYN
                     PFSQSFLLASKAPTVGKPDAERYIYEFTKTSIHSPQGYVTIKFDEIRAFIAKTYSYIQ
                     KTNFEINPSGIAIHPQTKDLYVLSALDRCIAVYSEKKLQHVYVLPAEIYYKPEGIGFL
                     PNGDMILSSEGMKNGYIGGQIFVIPQKK"
     gene            8393..8620
                     /locus_tag="P5243_00135"
     CDS             8393..8620
                     /locus_tag="P5243_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17882.1"
                     /translation="MVQIFILSIVLVGIAILGLGVNIFFRKQKFPETEVGKNKNMRAL
                     GLSCVKCEEMRKFREAQKYKNLKIDVAKLQL"
     gene            8621..9805
                     /locus_tag="P5243_00140"
     CDS             8621..9805
                     /locus_tag="P5243_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS17883.1"
                     /translation="MLAHNIYKLSVIKFAKWFMLYMPIIGLFYADNNLSAFQLFIIQA
                     GYSFSSAVFEIPSGYFADVVGRRKTLIIGSCMGTLGFTIYALSGSFVAFLCAEIIMGI
                     GQSFISGTDSAMLYDSVQHIQQKDAYLKYEGRITSLGGFAETGAALLGGIIAGVTSLH
                     SVFVVQACIAGLAIPAALLLQEPPRTKLIHTGISQVFRISYQSLFVNKKLRTSILFSS
                     GIGMATLAMAWTLQTYFVYHNFTETQTTTLWVLYNGLVAAISLFAAYIISKIGEKPLL
                     TSICVIVPLGFISLGYTPVAFALSFLVLFHIVRGYATPILKDMIQNDCEPEIRATILS
                     LRGMIIRLGFAIVGPVIGFMSGKFSFEFAVTVAGILFLVTAGTLLTMYWASHVYTNKN
                     NW"
     gene            10055..10453
                     /locus_tag="P5243_00145"
     CDS             10055..10453
                     /locus_tag="P5243_00145"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRS17884.1"
                     /translation="MLLLSIKSTETTPQVVFDIEKNIFEISGSSRPENISAFYTPIIN
                     TIETHFQSILQKNLTEFPPQFTFNFRLYYFNSGTSHFIASLFQLLNSYTQYGIASQIH
                     WYYDDEDMDMYEAGEELSFLTTIPFHFIAE"
BASE COUNT         3646 a         1599 c         1901 g         3581 t
ORIGIN      
        1 gctgttgaag aaaatgaaag gataaaagta caagaatttg aaagtgaaaa aacaaatgaa
       61 atatttcaag taattcagat tgcgggtgat aaaatgccta taggtatata ttctgaacaa
      121 aaatcatttg aaagtgttag atttcaatta atacctaacg atagtgttta tgtgttttct
      181 gatggatatc aagatcaatt cggcggcgaa aaaaataaaa aatttatggt aaaacgatta
      241 aaacaattgt tggttaacat aaatgccgaa tctatggaaa aacaaagaga aattcttgat
      301 aaagagcttg tagcttggat taagcaagga aataccgaac aagttgatga tattttagtt
      361 attggtttta ctgtacatta aataatataa ggcacctata tgtttgccta ttcactttct
      421 gatatagctc acattactaa cagtttttgt aaaggcaatt tacaaagtat tattacttca
      481 atttatattg atagtcgtga aacgcatagc aattcaggtt ctatgtttat ggctattccg
      541 gggaaaaaat tcaatggcca ttcttttata caaaaactgt atgaagaagg aattcgaaat
      601 ttttgggtac aaaacggaga atcgtatcct gaatttgatg atgcacaata tattatttct
      661 cataatgtaa tagatgcatt tcaattatta atgaaacact atcggttgca attttcaatc
      721 cctatagttg gtattaccgg aagtaatgga aaaactattg taaaagaatg gttgtattat
      781 gtgtgtcggc attcaatgtc ggtagttaga agtcctcgca gctacaattc gcaaataggt
      841 gttccattat ctttattatt gcttaattca aattatacac tcggtatatt tgaagcaggt
      901 atatcgcaac ctcatgaaat gcaaaaatta gcagcaatgc ttcaacctac tattggtatt
      961 tttacaaata ttggtgatgc acatcaagaa cattttattg atatagagca taaagtgact
     1021 gaaaaaatga aattgtttac tcatgtttct caattaattt attgtaaaga ttatgaattg
     1081 atagataaac atgctcagat gctgctaccc aatacgaaat tatatgcatg gtcatttatt
     1141 gatacatcaa gttctatttt tgtacagtat tctacacaaa aacatgcaac acatatttct
     1201 gtaaattatt attctactgt gtattcgttt gaaataccct atcatgatag ggcaagtata
     1261 gaaaacgctg ttcattgttg tattacagct ttagttttag gtattgaaac acaatatata
     1321 cagtatttta gtgatcttcc acaagttgca atgcgtcttg aacaaaaaag tggaaaacat
     1381 aattgtacta ttataaatga tagttataat tcagatttac aaagtcttac tgtggcactt
     1441 caatttttgt cacaacaaaa tcaacacccg caaaaaacac ttattttatc ggatttaatg
     1501 caaataactg ccgataaaaa agaattgtat tcacacattt catcactaat agtaacatat
     1561 ggtatttcaa aatgtatagg tataggtaaa gatgtgcaaa tatatttaaa accaaagatt
     1621 cagaaaggaa tattttttaa ttcaacagct gaatttttag acgcatatac tacacttgta
     1681 tttaacaatg aagctatttt attaaaaggt gctcgcgagt ttgaattcga aaaaattgca
     1741 aaaaaattag aatttcaaac acatcaaact gttcttgaaa ttaatttgaa tgccataatt
     1801 cataatttgc aatattttag aacctattta caggttcata ctaaagtaat ggtaatggta
     1861 aaagcgtttt cgtatggaag tggaagttat gaaatagcca atatactgca aaattataaa
     1921 gtagattatt tagcagttgc ctttgctgac gagggaatag aattacgaaa tgcggggatt
     1981 actctaccta ttgttgttat gaatcccgaa accggaatga tgcagcaaat ttttgaacat
     2041 ggtcttgaac caagtattca taatagtaca gttttagagg aatttgccga atattgtttg
     2101 gcgcattcag atagaatatt tccatatcat attaaaattg acaccggaat ggcacgatat
     2161 ggtttttcgc ccgatgaaat tctttctttg attgaacgag tacacgcaat tcccaatggt
     2221 acgataattt cggttttttc tcatttggta ggaaccgatg actcacagtt tgatgctttt
     2281 actgaacagc aaatagctac gtttgttcgc tccagttctc ttatttgttc gcagttttca
     2341 tatccaatca tgaggcatat actcaattcg gcaggaattg aacggtttag taaatatcag
     2401 tttgatatgg ttcgtttagg tatagggttg tatgggataa gcgcacagca tgagcacaat
     2461 tgtatgcata taagtacgct taaaacattt atttctgaaa ttagaagtat gcctgcagga
     2521 actactgtag ggtatagtag aaaaggaaca atttctcgac aatcaaaaat tggagtgttg
     2581 cctataggat atgcagatgg attgaataga agattgagta atggcgttgg aacagtgttg
     2641 gtaaacaata ttgaagtgcc tataattgga aatatatgta tggatgcaac tatgatagat
     2701 attaccgatg tagatgctga aataggagat gaggttgtat tgtttggccc ccgattacca
     2761 attactgtta tggctcagcg attaggaaca ataccgtatg aaattattac atcaatttcg
     2821 cgaagagtca aaagattgta ttattatgaa taatacggtg ttattctccc catattgttg
     2881 ccactatttt tctagagcct ccatagtttc tatgttcaca taaatatata ccttgccaag
     2941 tacctaaagc taactttcct tgtgtaatag gaatacttac agaacaccct aaaacacttg
     3001 cttttatatg agcaggcatg tcgtcagccc cttcgtgtgt atgaataaaa tatctattcg
     3061 tatcgggaat tattgtatta aaaaaagttt caaaatcagt tcttacatcg gggtcagcat
     3121 tttcattaat agttaacgaa gctgaagaat gttgaataaa taagtgaaga atacctactg
     3181 aaaaggtttg tatttcgggt aggtggtcaa gaattgaatg agtaataaga tgaaaccctc
     3241 gagaacgagc tgtaagttgt attttttttt gaatccacat atgttaaaat gctgccataa
     3301 gtaaatcaat atttttatat ggtaagccgt atttattggc aatataacta ttggttaaaa
     3361 tgccattgta catatatact ccttgtcgaa cagcaaaatc atgttttaag aagtttttta
     3421 ctccacccat ttcagacatt tttaataaaa tagggattac tatattgctt aaagcaatag
     3481 atgacgtacg tgctgcacga gaggtaatat tgggtacaca gtaatgtgta acgccatatt
     3541 tagtgaaaat aggatttttg tgagttgtgc aagccgatgt ttcaaaacat cctccttggt
     3601 caatacttaa gtctacaatt acagaacccg gtttcatact ttttatcatt tcttcagaaa
     3661 cataaaaatt ttgtccgtct tctactaatt gaattgtacc aattactact tcggctgatt
     3721 ttaaagcttt ttgcagtaca ccactatgat aattagatgt aaatactcgt tgccctaatg
     3781 cttgttgaat actgataagg cgttgtaaat aagaatcaaa aattttaaca tgagccccca
     3841 ttcctatggc agcgcgtgcc gcaaattctg cagccgtgtt agctcccaat attacaactt
     3901 cggcagggct aattccggtg ataccaccaa gtaacacacc tttaccaaca ttagaattgc
     3961 ttaaatattc agcagcaaca agaattgaca tagttcctgc tatttcatcc atactgcgaa
     4021 ctacaggata attaccttca atatctcgta tgtaatcaaa tccaatagca tttatccgtt
     4081 tttgagtaag ggcatgcact tgttgtaatg ccgattcgtt tattaaaaat gaagataaaa
     4141 ttgtttgttt ttctcgtaat aatgatattt cctgtgaatt aaacggagca acttttataa
     4201 gtacgtctgc ttcatatact tccggtttag tatcgcaaat tattgcacct acctcactat
     4261 agtcttggtt ggtaaatttt gctctgtctg cagcatttcg ttcaaataaa accttattgc
     4321 ctgcttctac caataatttt acggcttgag gtgtaagagc tatcctgtgc tcatttacat
     4381 cggtttcggc aagtaatcct attgttatat tttgacgttg agtatttact tcaagcattt
     4441 cttcctgggg tagtaacgct cctcgtccaa atgaaaattg tgttccaaga tttcccattt
     4501 ttttgtaaaa atttgtataa aaatatgaat tttttcgtca taatcagtaa agtatttttg
     4561 taatttttag aattggcaaa atattaacat tatcatattt tttttgtgta taaaaagtag
     4621 tgtgtatgtt tttatttatc aactaaaaac aagtattttg caggcaataa taattacgta
     4681 ttaaatatat tattttcatg acacacaaga atatagtaga ccgctcagca gacaatctga
     4741 gaattttatc gatggcaatg gtagaaaaat ctaaatcggg acacccaggt ggagcaatgg
     4801 gaggtgttga ttttgtaaat attttatatt cagaattttt acaatatgac ccaaccgatt
     4861 ttacgtggat aaatcgtgat cgtttctttt tagatccggg gcatatgtca ccaatgttat
     4921 attctatttt acatatggtg ggtactattt caattgatga aattaaatca tttcgtcagt
     4981 ggggaagtgt gtgtgcaggg catcctgaag tagatcatgc tcgaggcatc gaaaacacat
     5041 cgggtcctct tggtcaaggt catacgatgg ctgtaggtgc tgcaattgca gaaaaattct
     5101 taacccaacg atttggggca tggatgaaac ataaaattta tgcatatatt tctgatggtg
     5161 gtatacaaga agaaatttcg caaggtgcag ggcgtatagc aggccatttg ggactcaata
     5221 atcttgtgat gttttttgac tcaaacgata tacaactttc taccgaaacc aaagatgtaa
     5281 ccagtgaaga tactgcaaaa aaatacgaag catgggggtg gcaaacgatt actataaatg
     5341 gtaacgacca acatgaaatt cgtaaggcat tacaattggc tcaaggagat ttagacaaac
     5401 cgctgctaat aataggtaaa accactatgg gtaaaggttt ggttgacgag caaggcaaca
     5461 gctttgaacg taaaacatct actcacggac aaccggttag taaagcaggt gcttctgttg
     5521 aaaaaacaat acttaattta ggtggaaatc cggctgaccc atttgcggta tttgatgatg
     5581 tagcagcata ttggcaacaa ataaaacaac aaaaaattgc tcaggctcag gcaaaaaaac
     5641 aattgcaaac tgcatgggaa aaacaaaatc ctgaattagc tcaaaaatta cgtgatttct
     5701 atgcaggcaa attacctgaa atagattatt caaaaattga acaaaaagca aatattgcaa
     5761 cccgtgtagc atctaaaaca gtattagaat attttgcaga acatattgaa aatatgatag
     5821 tttcatctgc tgacctttct aatagtgaca ataccgacgg atatctgaaa aaaacaaaag
     5881 ctctgaaaaa agatgatttt tcgggtaaat ttttagaatc cggtgtggca gaattgacta
     5941 tggcagctat tatgaacggt atggtattgc atggtggtgt tattgctgcg tgcggaacat
     6001 tcttcgtatt ttctgaatat atgaaacctg ctatgcgtat gtcggctctt atggaattgc
     6061 ccgtaaaata tatttggacg cacgatgcat tccgtgtagg agaagacgga ccaactcatc
     6121 aaccaataga acaagaagct caggtgcgac ttatggagca tgtgtataat catagcggca
     6181 aacgtagttt tgttattctt cgacctgccg atgctgtaga agcgtctgta tgttggaaaa
     6241 tggcaatgga aattaacaat cgtccggttg gcttaatttt ttcacgccaa gtaattaaag
     6301 acattccggc aaaacaagga agtactcgat ataccgatgc attacaagca tacaaagggg
     6361 catatattgt aaacgaacca caacgtgaac cggatttaat aatggttgca aatggttccg
     6421 aagtttctac aatattagaa ggggctcaca tactcgaaga gcaatatggt ttgtgtgtac
     6481 aagttgtatc tgcaccgtct gaaggattat ttttagacca agacgaagcg tatcgcaaca
     6541 aagtattacc gcaacataaa cctattttag gatttactgc aggcttacct ataaacttac
     6601 gtggtcttgt aggtccaaac ggaaaagtat ggggattaaa tcattttggc tattctgcac
     6661 cgttcgaagt attggacgaa aaatttggat acaccaaaga aaatgttgtg aaacaagcgg
     6721 tggaatattt ggggttgtaa tttgattcaa tcaattattg taatttattg atttacaaag
     6781 caaatcttat ttgctttgta aactgcgaag tgttattatt attgattttt tattcaaacc
     6841 attaaaaggt aaaataatac tattattctt tcgtgatttt tataaataca atgttatttt
     6901 ttctaaaata atactatctc attgatatga taaaacattt ctaattcttt atttgcaatg
     6961 atatacgaca gatacaaatg ctgaaatttt ctatgcaaat accaataatt tttgtaattt
     7021 tgagaaaaag aaaatttata cgatgaagat attaagtaaa caaatagcaa taagcattat
     7081 aatgatgtat gctttagttt catgttctca aactaaatct ttgcataatt ataaagcaat
     7141 agaaactatc tgcaaggatg cagacatcat tgaagtagaa aaaaaaattg attatactga
     7201 agtagaattc acttgcaaag gaattaatta tgaagtaggt attgatttac aagggaatat
     7261 aatttatacc gaaaaagcag ttagccaaaa cgaaataccg tttgaaaata ttcaaaagaa
     7321 aattataaaa aaatatccag attggagtat tgatgagttt tctatcgtag atttacccga
     7381 tacgagtttt ataaaagtag aattattaag aaacggaatt gaagaaaatg ctttttttac
     7441 actcgatggc aagtggttta aacctaaaaa ttatgttggt aaagacacgt ggaatataca
     7501 cgatttatct gagactgtat attattcagc ttcggcatat aattatttga acccaacaac
     7561 tataatagat ttacccgaaa cacttcgcga aatttccgga attgctgttg taaataatac
     7621 taccctatta tgtgtgcaag atgaacttgg agctgtattt agtctagatt tgcaaaaaca
     7681 agaaataact aatatgttcc gatttaccga tattggtgat tttgaagata ttgcgtataa
     7741 taacgatact gcttacgtat tgcggagcga tggaactatt ttttcatttg catacacaaa
     7801 tttcaccggc aacattcaac aaaaaattgt tcaggcaagt agcttaaata tcgaaggctt
     7861 gtgttataac cctttttcgc aatcattttt acttgcctct aaagcaccta cggtagggaa
     7921 acccgatgct gagcggtata tttatgaatt tacaaaaaca agcatccatt cacctcaagg
     7981 gtatgttact ataaaattcg acgaaattag agcatttata gcaaaaactt attcatatat
     8041 acaaaaaaca aatttcgaaa taaatccttc gggtattgct attcatccac aaaccaaaga
     8101 tttatatgta ttgtccgcat tggatagatg tattgcggta tattccgaaa aaaaattaca
     8161 acatgtgtat gtgttacctg ccgaaatata ttataaaccc gaaggtattg ggtttttacc
     8221 aaatggtgat atgatattat cgagcgaagg aatgaaaaat ggatatattg gcgggcaaat
     8281 ttttgtgatt ccgcagaaaa aataaaataa taattttgta attatcatag gtaaaaaatg
     8341 tactaataaa aaatgaaata ctttcatact ttataaactt ttaaacaaaa aaatggtaca
     8401 aatatttata ttatcaatcg ttttggttgg cattgcaatt ttaggattgg gagtaaatat
     8461 cttttttaga aaacaaaaat ttcctgaaac cgaagttggc aagaataaga atatgcgagc
     8521 tttaggttta tcgtgtgtaa aatgcgagga gatgcgcaaa tttagagaag cacaaaaata
     8581 taagaatcta aaaattgatg tagctaaatt acagctgtaa atgcttgcac ataatatcta
     8641 caaactttcg gttataaaat ttgccaaatg gtttatgctc tatatgccta ttatagggct
     8701 tttttatgcc gacaataatc ttagtgcatt tcaattattt ataattcaag ccgggtattc
     8761 atttagtagt gcggtatttg aaataccttc ggggtatttt gctgatgtag ttggtcgtcg
     8821 caagaccttg ataattggta gttgtatggg tacgcttggt tttacaatct atgcattgag
     8881 tggaagtttt gtagcatttt tgtgtgccga aattattatg ggaattgggc aaagttttat
     8941 atcgggaacc gattctgcta tgctgtatga ttcggtgcaa catatacaac aaaaagatgc
     9001 atatttaaag tacgaaggta gaattacttc gcttggtggc tttgccgaaa cgggagctgc
     9061 cctacttggt ggtatcattg ctggtgttac atcattacac agtgtatttg tagtacaagc
     9121 ttgtattgca ggattagcta taccggctgc tctattattg caagaaccgc ctcgaaccaa
     9181 acttatacac acaggtattt ctcaggtgtt tcgtattagt taccaatcgt tgtttgtaaa
     9241 caaaaaatta cgtacaagta ttttgttttc atcgggtata ggcatggcaa ctttagcaat
     9301 ggcatggaca ctgcaaacgt attttgtgta tcataatttt accgaaactc aaactactac
     9361 attatgggta ttgtataatg gattagttgc tgctatttcg ttgtttgctg catatatcat
     9421 ttcaaaaatt ggagaaaagc cgttacttac aagtatatgt gttatagtac cattaggatt
     9481 tataagtttg gggtatactc ctgtagcgtt tgcattgtca tttttggtgc tatttcatat
     9541 tgtacgcgga tatgcaacac caatacttaa agatatgata caaaatgatt gtgaacccga
     9601 aataagagca acaatattgt cattacgagg aatgattata cgattaggtt ttgctattgt
     9661 gggacctgtt attgggttta tgagtggaaa gtttagtttt gaatttgcgg taaccgtagc
     9721 aggtatttta tttttagtaa cagccggcac cttattaaca atgtattggg cttcacatgt
     9781 ttatacaaac aaaaataatt ggtaactatt tctgttctaa tttgatgata tttatcatca
     9841 aatatattgt gttttaatat ttttttattt gaatagtaat tgtaattatt ttaattttaa
     9901 atacttatat ttttattata taatggaata tgtgtaaaaa taaatatgta catatactca
     9961 caaaaagttg gtttgccaat gaaaaaaagt aaaaaatcat tatatttgtt tttagattag
    10021 gatgtttatt ttcgatactg ttttttttgt tattatgtta ttgctttcaa ttaaatcgac
    10081 agaaacaact ccacaagttg ttttcgatat tgaaaaaaat atttttgaga tttcaggaag
    10141 ttcgcgtcct gaaaatatat cagcatttta tacgccaata atcaatacaa tagaaacaca
    10201 ttttcaatca attttacaaa aaaatcttac ggaatttcca cctcaattta cctttaattt
    10261 cagattatat tattttaatt caggtacatc acattttata gcaagtcttt ttcaactact
    10321 caactcgtat acacaatatg gtatagcgag tcaaatacat tggtattatg atgacgaaga
    10381 catggatatg tacgaagccg gcgaagaatt atcgtttcta acaactatac cgtttcattt
    10441 tatagcagaa taattgtcat tattattatg cttttaaata tatattctta atgcctaaat
    10501 tcaaattttg tgaggctgtt ttttttgacc atattagtag cattattgta tgtccctata
    10561 tacagtagtc ccatactatg ttcagttgat tctgaaaaca actttgaatt tgtaattagt
    10621 cacgttcata tttttgaaga tacaagcaat tctgttacta tacaagatat ttttaatcat
    10681 tattgtccga taaaactaca aaattaattt aataaaacaa aatttat
//
LOCUS       DAOTOW010000004        10589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_5879_length_10589_cov_3.795899, whole genome shotgun sequence.
ACCESSION   DAOTOW010000004 DAOTOW010000000
VERSION     DAOTOW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_10589_cov_3.795899"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="P5243_00150"
     CDS             complement(<1..823)
                     /locus_tag="P5243_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS17885.1"
                     /translation="MMKHISIYTFRIIFLSLLFVSPLVLTSYDDTRNFEISKNLDIFY
                     SVFQEINTHYVDQIDPGELMSYAIDKMLESLDPYTKYIPESDVERFQMMTKGEYGGIG
                     AMIGKRDSVMMISEIFEGYPAHKAGLLPGDKIVTIDNRSIIGKNRDEIHELLQGQPGT
                     TIILGIQRGNSKTINTYSVKRETINLKSVPYYNMLDNNIGYVKLNQFTQNCAVEVKNA
                     CNDLRLKGAKSLILDLRDNPGGLLMEAIQITNLFVEQNKKIVYTKGKNKIAESTFY"
     gene            complement(894..2174)
                     /locus_tag="P5243_00155"
     CDS             complement(894..2174)
                     /locus_tag="P5243_00155"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRS17886.1"
                     /translation="MFIILIMPYMALSINNIKQLDSTIRDFGSLFLSNYSGTVSIEDF
                     MIQFHEMQSFAPVQFDSVYVLKTFTIQTEKARRVFEYTKQQANNFTQILSYDTITRAI
                     GNHTYLYVSIVYDVASPLVELQQKRRVFAKFVISGSTLKLWGKVQIMTDLEYYRTELQ
                     EVYDDRYSYFNKTYKDSGIAYIDTTFGFIPFQIKNLWGLQSLNGIVIIQPQFDSLYAF
                     RGGVALVKKAGKYNLLKPDGSFVLSKWNSHIYCTMQDDNYVYWQKKSKNTYARIDFTY
                     IPPKEIDNSKYTKQYYGSKNIVYSIADDPCHAQCYNIINTRTQLKIAEFKAMQLFERM
                     GDFFFAVKQDTSLVIDSAGVILFQSPYVCMAQFPGNVIVYNRNTRLFGMYCPYTKQYI
                     VPVYSFILPVERDKFFVALTRNYEFEYIVGEHSK"
     gene            complement(2203..2694)
                     /locus_tag="P5243_00160"
     CDS             complement(2203..2694)
                     /locus_tag="P5243_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17887.1"
                     /translation="MKYGVLFCIAAVLCIQLHAQPQARTGATRNTIYVSGGTRITFVS
                     ASFGYERLLIVNPAFQLAARANIGTYMKIMVDEGAFVSSSLVTLHGKRYHLFETGLGA
                     CLFRSQFGFAKEENGVYTEVPYKWYPAVYMGYRYKNPRKHYMFKAGVGWPEQISAGIG
                     ISF"
     gene            complement(2699..4435)
                     /locus_tag="P5243_00165"
     CDS             complement(2699..4435)
                     /locus_tag="P5243_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRS17888.1"
                     /translation="MMRKILMGAIALPLILSISCSKKSTQSSHTSIAMDTSWKQLTLR
                     EKIGQTMMVTTDLYAQKKIGNGSLQEFFAKYPIGGFFVAQWHFTYQKPDDLIFEEFIP
                     RIMREYDSASKFPLFFSEDFERGAGYNYNTCTKLPVLMTLGAANSKDLAYAYGKIVSA
                     ESRSMGFNWLLHPVADLNMNPLHPLVIERAISDNAELALPLLQSQLQAMKDQNVISTI
                     KHFPGDGVTICDQHLITSANNLPMDQWRKTFGKVFQTLIDEGVPTIMVGHLQFPAYQK
                     ETIHGELPPATLSREIMQQLLKQEMGFKGVVVSDAMNMGGASGFYDNALEASVKCFEA
                     GADMILWPSLAYMDTVEARILRGEIPMQRLDDAVERIWALREKYGLLTKKATISTPLP
                     PNHAQYVQEKMTELATKAVTLIQDKNNDIPLSPNKTKKIFMVNISHNDKRAVLQTMKK
                     ELESRGFQVRLEHDFHLYNWEWRWDSLAQYDKFIVCFENKYFDPIGSPLLKDKEAYCL
                     WSIKNLPKEKIISVSFSNPYYNTFYLKTAPVLINAYSSDDYMQKAVVQTLMGEIKPTA
                     KSPVNLYHQNMK"
     gene            complement(4585..5238)
                     /locus_tag="P5243_00170"
     CDS             complement(4585..5238)
                     /locus_tag="P5243_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17889.1"
                     /translation="MNDVKIQWFDVPDESVDNYDVHNKSFAVGGAGNYIYNDAIIRFR
                     YNQTSLFLQEYQEVDDYNLMTTAFNICEAQQKKHTIAPAVLWKTKEDKFQLYAGFEIP
                     VTFHGEYTFTVTNSIYELGTKTIIEKTQTKHTIPKGYTCGIGGIFGFTVFLYKNFSIG
                     GEFSPSLLYSKVGGKTHGTFYNIVPEPQFSVELGTKDEMRGITFVEQRFSIGIGVWF"
     gene            complement(5386..6084)
                     /locus_tag="P5243_00175"
     CDS             complement(5386..6084)
                     /locus_tag="P5243_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS17890.1"
                     /translation="MKCVLVDDEPLARDVLQTYIEQVPYLQLIGICKNVFEASQYLSK
                     ESIDLLFLDIQMPEINGVAFAHSLQNAPMIIFTTAYSQYAIEGFDLQAVDYLLKPISP
                     ERFLKAVTKAYEIYMFKNQIHNQEINQDFMFVKSEYQTVKINFNDITYIEGLKDYVKI
                     YCGKKMIVTLMNMKHIYEKLPKNLFIRVHKSYIIHIHAIEKIDRQRILIQSKYIPIGD
                     VYKDEFEKKMGLIS"
     gene            complement(6134..7240)
                     /locus_tag="P5243_00180"
     CDS             complement(6134..7240)
                     /locus_tag="P5243_00180"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRS17891.1"
                     /translation="MNVNFKNVLFVIAHILFWILFITVPHIVIGIPFQFQFFKFKLYE
                     TLVLIILFYLNAYVLVPKFLFVKKYILYIVCVTVIILSGMHISKLFMPPKIPRNSMPF
                     EMEKPEIQHNLQLIESAEYKDMKHNYLHNKFKRIEQKRANFIGLLLLVIFALSTGYTY
                     AIQMYAYETQMKQLQHAHIESQLQLLKSQINPHFLFNTLNSIYSLSLKKSDVTPKAIL
                     QVSEMLRYMTYDAQNQFVPIEREIAYIQNYIALQKLRVSSESILEFSVTGVTSNVQIA
                     PMIFLPFIENACKHGISKDSTLTMCSTITITETKIYFTVTNTLYNIGNENENKGIGIT
                     NVSQRLQALYPGKHILDIAKSNDEFTVSLQIQYV"
     gene            complement(7368..7805)
                     /locus_tag="P5243_00185"
     CDS             complement(7368..7805)
                     /locus_tag="P5243_00185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRS17892.1"
                     /translation="MKPEDILQYTFNHAHPIQVRYNDIDLAGHVNNAVYHEFFDLGRT
                     FYFKEVLGEKPFTYEQNIVIAQSNTTYVKEVFLEDELQIVSKIIRFGNKSFDMIQALL
                     RNTPEGAELTTYNLTTFVCMNYSKHEPQLIPLVWKDKITKFEK"
     gene            complement(7807..8370)
                     /locus_tag="P5243_00190"
     CDS             complement(7807..8370)
                     /locus_tag="P5243_00190"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRS17893.1"
                     /translation="MKEDIEKSLDVLYAGGIFLYPTDTVWGLGCDATNEVAVKKIYAA
                     KQRNEQVPLLVLVNSIAMLERYVEEVPQVAYDIIELSEKPITIVFEKGKNLAKNCLNS
                     DGSIGIRVTNEQFTNQLITRFKRPIISTSANIHGQPTPSIFNEISIEIKSAVDYIVKY
                     RQNDVSPTQASSIIKIKNNSEITIIRK"
     gene            complement(8386..9621)
                     /locus_tag="P5243_00195"
     CDS             complement(8386..9621)
                     /locus_tag="P5243_00195"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRS17894.1"
                     /translation="MVAVEREVFFELPNKAKVVLSKSNSLVSYICLFVHSGSRDEPVG
                     CDGVMHVIEHMLFKGTQTRNYYELMSLIESVGGDMNAYTSKEETCIYITIQNQYLERA
                     VDVLHDIFYNSQFLQQEFIKEREVILDEINSYKDTPGELIFDEFEECFFQDHELASNI
                     LGTEDSLMQMSVEKVREVYTREYIPQKLIISYVGGVDLDVVQLYIEKYFTKDKNTTLQ
                     LSKRIPFEGTPRFTKIIQKDTFQTHCMIGEQAPGMYDSNKTAMVLLNNLLGGNSFNAK
                     LNLALREERGLTYNIESNYTAYYDTGIFTVYFGTESTKINESKKIIFSIFDTVIQTGL
                     THDELAMYKQQLIGQVALSFDNFVSLMISNAKSYMCYDKVDTYQEIIEKINAVSNEQL
                     QNLAQSVLNKQYISCLEYI"
     gene            complement(9700..>10589)
                     /locus_tag="P5243_00200"
     CDS             complement(9700..>10589)
                     /locus_tag="P5243_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS17895.1"
                     /translation="IPAFSNIEIYRPGYAIEYDYFDPTQLQASLETKMVKNLFFAGQI
                     NGTTGYEEAAAQGLIAGINAHAAITNKEPLVLKRDEAYIGVLIDDLITKGVNEPYRMF
                     TSRAEFRILLRQDNADVRLTPVGIVIGLVDDYRKKVFENKYKVIETIKDYVKSTTIHM
                     NKANEILEQHGSSPLKQSMKMSALISRPNIYASMFPIPEDLLNQCETDKETLLEVVDI
                     DIKYSGYIEKEKQIAEKISRLEYIKIKGKIPYMEMPSLSTEARQKLTEINPETIGQAS
                     RISGVSPADISVLLVYLGR"
BASE COUNT         3563 a         1837 c         1407 g         3782 t
ORIGIN      
        1 tataaaatgt agattctgct attttatttt ttcctttagt gtacacaatt tttttatttt
       61 gttccacaaa caaattagta atttgaattg cctccataag caatccaccg ggattatcgc
      121 gcaaatcaag tataagtgat tttgctcctt ttaaacgtaa atcattacac gcatttttta
      181 cttctacagc acaattttgt gtaaactgat tcaactttac atatcctata ttattatcga
      241 gcatattata ataaggaaca ctttttagat ttatagtttc gcgttttacc gaataagtat
      301 taatggtttt tgaattacct ctttgaattc caagaattat agtagttccg ggttgtcctt
      361 gtaataattc atgtatttca tcacgatttt ttccaataat tgatctatta tcaatagtca
      421 caattttgtc accgggcaac aaaccggctt tatgagcagg atatccttca aaaatctcag
      481 atatcatcat tacactatcg cgtttgccta tcatagcacc tatgccgcca tattcacctt
      541 tagtcatcat ttgaaatcgc tcaacatcag attcaggaat atatttagta taagggtcta
      601 gagattcaag cattttatca attgcataac tcatcaactc tcccggatct atttgatcta
      661 catagtgagt atttatttct tgaaaaaccg aataaaaaat atcaagattt ttcgaaattt
      721 cgaaatttcg ggtatcatca taacttgtaa gtacaagagg cgacacaaac aaaagagata
      781 aaaatataat tctaaacgta tatatagaaa tatgtttcat catagtatta ttgtaatttt
      841 tcaaatgtat aaaataacaa aattaatgta atcataaaaa tgtaaaaaac gtatcatttg
      901 ctatgttctc ctacaatata ttcaaactcg taattacgtg taagtgccac aaaaaacttg
      961 tcacgttcta caggtagtat aaagctatat acgggaacta tatattgttt agtgtatgga
     1021 caatacatgc caaacaaacg agtatttcta ttataaacaa taacattccc cgggaattga
     1081 gccatacaca catatggcga ttgaaacagt ataacaccgg cagaatcaat cactaatgac
     1141 gtatcttgtt ttactgcaaa aaagaaatca cccattcgtt caaacaattg catggcttta
     1201 aactcggcaa tttttaactg tgtgcgtgta tttattatgt tataacactg tgcatgacac
     1261 ggatcgtcgg caatagaata tactatattt ttggaaccat aatattgctt agtatactta
     1321 ctgttatcta tttcttttgg gggaatatat gtaaaatcta tacgtgcata tgtattttta
     1381 gatttttttt gccaatacac ataattatcg tcctgcattg tacaatatat atgtgaattc
     1441 catttagata acacaaatga accatcgggc tttagcaaat tgtatttacc tgcttttttc
     1501 accaaagcta ctcctcctct aaaagcatac aaagaatcaa actgaggttg tataattacg
     1561 attccgttta acgattgtaa cccccaaaga tttttaattt gaaatggaat gaaaccaaac
     1621 gttgtatcaa tatatgcaat accggaatct ttataagtct tattaaaata agaatatcta
     1681 tcatcataca cttcttgcaa ttcggttcta taatactcca aatcggtcat aatttgtact
     1741 ttaccccata atttaagagt agaacccgaa ataacaaatt ttgcaaacac acgtcgtttt
     1801 tgttgcaatt ctactagagg cgatgcaaca tcatacacaa tagaaacata aagatacgta
     1861 tggtttccta tagcacgagt tatagtatca tacgatagaa tttgggtaaa attatttgct
     1921 tgttgctttg tgtattcaaa aacccttctg gccttttctg tttgtatggt aaacgttttt
     1981 aaaacatata ccgaatcaaa ttgcacagga gcaaatgatt gcatttcatg aaattgaatc
     2041 ataaaatctt caatgcttac agtaccggaa taatttgata aaaacaatga accaaaatct
     2101 cgaattgtag aatcaagttg ctttatatta tttatgctta atgccatata aggcattata
     2161 agtataataa acatatgcac tattcttttt ctcataccag tattaaaaag atatacctat
     2221 tccagctgaa atttgctcgg gccaccctac tcctgcttta aacatataat gctttcgcgg
     2281 atttttatat ctatacccca tatacacagc aggataccat ttatacggca cttcggtata
     2341 tactccgttt tcttcttttg caaaaccaaa ttgagaacga aataaacatg ctcctaaacc
     2401 ggtttcaaac aaatgatacc gtttaccatg aagagttacc aaactcgacg aaacaaatgc
     2461 tccttcgtca accataattt tcatataagt accaatatta gcacgagcag ctaattgaaa
     2521 agctggattt actattaata aacgttcgta accaaaactg gccgatacaa aagtaatacg
     2581 agtacctccg cttacatata tagtatttcg tgtagctccg gtgcgagcct ggggctgagc
     2641 atgcaattga atgcatagta cagcagcaat acaaaacaac acaccgtatt tcatgttttt
     2701 atttcatatt ttgatgatat aaatttaccg ggctttttgc tgtcggtttt atttctccca
     2761 taagcgtttg aacaactgct ttttgcatat aatcatcgct gctatacgca tttattaaaa
     2821 caggtgctgt ttttaaataa aacgtattat aatatggatt actaaatgaa acacttatta
     2881 ttttttcttt cggcaaattt ttaatactcc ataagcaata tgcttctttg tctttaagca
     2941 atggtgaacc tataggatca aaatatttat tttcaaaaca cactatgaat ttatcatatt
     3001 gagccaatga atcccaacgc cattcccaat tatataaatg aaaatcatgt tctaaacgta
     3061 cttgaaaacc tctactttct aattccttct tcatagtttg caacaccgca cgtttatcat
     3121 tatgactaat atttaccata aatatttttt tggttttatt aggtgaaaga gggatatcat
     3181 tgtttttatc ttgtatcaat gtaaccgctt ttgtggccaa ttccgtcatt ttttcttgaa
     3241 catactgtgc atgattagga ggaagcggag ttgatatagt tgcttttttt gtaagtaatc
     3301 catatttttc tcgcaaagcc caaattcttt ctaccgcatc gtcaagtcgt tgcataggaa
     3361 tttcgccgcg taatatacga gcctcaacag tatccatata tgctaacgaa ggccacaata
     3421 tcatatcggc gccggcttca aaacatttaa ccgaagcttc taatgcatta tcgtaaaaac
     3481 cagatgctcc tcccatattc atagcatcag atactactac acccttaaat cccatttctt
     3541 gttttaataa ttgctgcata atttcgcgcg ataaagttgc cggtggcaat tcaccatgta
     3601 tagtttcttt ttgataagcc ggaaactgca aatggccaac cataattgtt ggaactcctt
     3661 catctataag agtttgaaac actttgccaa atgtttttcg ccattggtcc attggtaaat
     3721 tatttgccga agtaattaaa tgttgatcac aaatagtaac tccatcgccg ggaaaatgtt
     3781 taatggtaga aattacattt tgatctttca ttgcttgtaa ttgactttgc aacagaggca
     3841 atgctagttc tgcattatca cttattgcac gttctattac caatggatgc aaaggattca
     3901 tatttaaatc ggcaacagga tgtagtaacc aattaaaacc cattgagcgt gattctgctg
     3961 acactatttt tccgtatgca tatgccaaat ctttactatt tgcagcacct aaagtcataa
     4021 gcaccggcaa ttttgtgcat gtattgtaat tataccctgc acctcgttca aaatcttcac
     4081 taaaaaataa cggaaatttt gatgcagaat catattcacg cattatacgt ggtataaatt
     4141 cttcaaaaat taaatcatcc ggtttttgat aggtaaaatg ccattgtgcc acaaaaaaac
     4201 ctccaatagg atattttgca aaaaattctt gtaaactccc gtttcctatt ttcttttgtg
     4261 catacaaatc ggtagtaacc atcatggttt gacctatttt ttcccgtaat gttaattgtt
     4321 tccacgatgt atccatagct atagatgtat gtgaagactg tgttgatttt ttagaacacg
     4381 aaatactcaa aataaggggt aatgcaatgg ctcccatcaa aatttttctc atcataaaca
     4441 aacctttttt aaattaaaaa aaatcaatag catatcatac tatagactta actacaataa
     4501 tactattttt tttttatgta ataaacgaga atataatatt ttacaataat attttacaat
     4561 aatattctat tacgaataat ttacttaaaa ccaaacacct attcctatcg aaaagcgttg
     4621 ttcaacaaat gtaattcctc tcatttcatc tttcgttcca agttcgacag aaaattgtgg
     4681 ttcgggaaca atattgtaaa atgttccatg agtttttcca ccaactttag aatacaacaa
     4741 agaaggggag aattctccac ctatagaaaa attcttatac aaaaaaactg taaaaccaaa
     4801 aatacctcca ataccacaag tatacccttt aggaatagta tgttttgttt gtgttttttc
     4861 aataattgtt ttggttccta attcatatat actatttgta acagtaaatg tatattcacc
     4921 atgaaaagta acaggtatct caaaaccggc atacaattga aatttatctt ctttggtttt
     4981 ccatagtact gccggagcta tagtgtgttt tttttgttga gcttcacaaa tattaaatgc
     5041 agtagtcatc aagttataat catctacttc ttgatattct tgtaaaaaaa gcgatgtttg
     5101 attgtaacga aatcgaataa ttgcatcatt atatatgtaa ttacctgctc cgccaacagc
     5161 aaatgattta ttatgaacat cataattatc aacggactca tccggaacat caaaccactg
     5221 tattttaaca tcattcacag gattacttac aaatatttgt gccccttttg aaggtgtttg
     5281 agctgttaca ctaattgaat aagcaagaat actaaaaaaa cgttagaatt tttctcatta
     5341 tagttatatg ttgatttaca aacaaatata catatttatt gcaaattatg aaattaatcc
     5401 catttttttt tcaaattcat ctttatacac atcacctatt ggtatatatt tagactggat
     5461 aagaattctc tgacgatcta ttttttctat ggcatgtata tgaataatat atgatttatg
     5521 aactcgaata aagagatttt taggcaattt ttcatatata tgtttcatat tcataagtgt
     5581 gacaatcatt tttttaccac aatatatttt aacataatct ttaagtcctt caatataggt
     5641 aatatcatta aaatttattt taactgtttg atattctgac tttacaaaca taaaatcttg
     5701 atttatttct tgattatgaa tttgattttt aaacatatat atttcatatg cttttgttac
     5761 tgcttttaaa aaacgttcag gagaaattgg ttttaacaaa taatcaactg cttgtaaatc
     5821 aaatccttct atagcatatt gagaatatgc agttgtaaaa attatcattg gcgcattttg
     5881 tagcgaatga gcaaatgcaa ctccgttgat ttctggcatt tgaatatcta aaaacaaaag
     5941 atctatggat tctttactaa gatattgaga agcttcaaaa acatttttac aaataccaat
     6001 caattgcaaa tatggtactt gctctatata agtctgtaac acatctctgg caagaggttc
     6061 atcatcaact aacacacatt tcataaatca ttaattttca agtatttaaa caacaagaaa
     6121 acatggttct agtttataca tattgaattt gtaaggaaac agtaaattca tcattacttt
     6181 tagctatatc taatatatgt ttacctggat acaatgcttg taagcgttgt gatacatttg
     6241 ttattccaat acctttgttt tcattttcat tacctatatt atataaagta ttagttacag
     6301 taaaataaat ttttgtttca gtaatcgtta tagtagaaca catagttaat gttgaatctt
     6361 ttgatattcc gtgcttacat gcattttcta taaacggcaa aaaaatcatg ggcgctattt
     6421 gcacattgct agtaacaccg gtaactgaaa attcaagaat actttcagat gatacccgta
     6481 atttttgtaa ggcaatataa ttttgaatat atgctatttc acgctctata ggaacaaact
     6541 gattttgagc atcatatgtc atatatctaa gcatttcgga tacttgtaaa atagctttag
     6601 gagttacatc agattttttg agactaaggg aataaatact attaagtgta ttaaataaaa
     6661 aatgaggatt tatttgagat ttgagcagtt gcaattgtga ttcaatatga gcatgttgta
     6721 attgcttcat ctgagtttca taggcataca tctgaatagc ataagtataa ccggtactga
     6781 gagcgaaaat tacaagaaga agtaaaccta taaaatttgc tcgtttttgt tcaattcttt
     6841 taaatttatt atgtaaataa ttatgcttca tgtctttata ttcagcgctt tctataagtt
     6901 gcaagttatg ttgaatttca ggtttttcca tctcaaaagg catactattt cttggtattt
     6961 taggaggcat aaatagttta cttatgtgca tacctgaaag tataataaca gttacacaaa
     7021 caatatacag aatatatttt ttaacaaata aaaatttagg aactaataca taagcattca
     7081 aataaaacaa aattataaga actaatgttt catataattt aaacttaaaa aattgaaact
     7141 gaaaaggaat tccgattacg atatgaggaa cagttataaa taaaatccaa aataaaatat
     7201 gtgcaattac aaacagtaca tttttaaaat ttacattcat ttgaacaatt ataaagtatt
     7261 attattcata tatataacct aataaaatta cgtactgtta aatatactat ttttcgttct
     7321 attttctaca ctcaaataga caaataaaca aaaagtatag acaaaatcta tttttcaaat
     7381 tttgttattt tatctttcca aaccaaaggt atcaattgtg gttcatgttt tgaataattc
     7441 atacaaacaa acgtggtgag attatatgta gtaagctcag caccttccgg agtatttcgc
     7501 aacaacgcct gaatcatatc aaaactttta ttgccaaaac gtattatttt actcactatt
     7561 tgcaattcat cttccaaaaa tacctcctta acataggtag tatttgattg agcaattaca
     7621 atattttgct cgtaagtaaa cggtttttcc cctaacactt ctttaaaata aaatgttcta
     7681 cctaaatcaa aaaactcgtg atacacagca ttatttacat gtccggctaa atctatatca
     7741 ttgtatcgaa cctgaatagg atgagcatga ttaaacgtat attgtaaaat atcttcgggt
     7801 ttcatattat ttacgtatta tggttatttc actattattt ttaattttta taatgcttga
     7861 agcttgtgtc ggcgacacat cattttgtct atacttaaca atataatcta cggctgattt
     7921 tatttctata gaaatttcat taaatattga cggggttggt tgtccatgaa tatttgctga
     7981 agtagatata ataggtcgtt taaaacgagt tattagctga ttggtaaatt gttcatttgt
     8041 tactctaata ccaatactac cgtcactatt taaacaattt tttgccaaat tttttccctt
     8101 ttcaaatact attgttattg gtttttctga taattcaata atatcataag caacttgcgg
     8161 aacttcttct acatatcgtt caagcattgc aatagaattt actaaaacta acaacggcac
     8221 ttgttcgtta cgttgttttg ctgcatatat ttttttaaca gcaacttcat tcgttgcatc
     8281 acaacctaat ccccaaactg tatcggttgg atacaaaaaa ataccacctg catacaacac
     8341 atcaagagat ttttcgatat cttctttcat atttactaat ataaattata tatactccaa
     8401 acaactaata tattgtttat tgagaaccga ctgagcaaga ttttgtaatt gttcattaga
     8461 cactgcatta attttttcaa taatttcttg atatgtatct accttatcat aacacatata
     8521 cgatttagca tttgaaatca taagtgaaac aaaattatca aaagacaacg ctacttgtcc
     8581 tattaactgt tgtttataca tagctaattc atcatgagtc aaaccagtct gaattacagt
     8641 atcaaatata gaaaatatta ttttcttact ctcattaatt tttgttgatt ctgttccaaa
     8701 atacacagta aaaatacctg tatcataata tgcagtataa ttagattcaa tattataggt
     8761 taaccccctc tcttcacgca atgcaagatt taattttgca ttaaagctat ttcctcctaa
     8821 aagattattt aataaaacca ttgctgtttt atttgaatca tacatacctg gagcttgctc
     8881 tccaatcata caatgcgttt gaaatgtgtc tttttgaata atttttgtaa aacgcggagt
     8941 tccttcaaaa ggtattcgtt ttgataattg aagtgtagta tttttatctt ttgtaaaata
     9001 tttttcaata tacaattgca ctacatctaa gtcaacacca ccaacatagg aaataataag
     9061 cttttgtggt atatattctc ttgtatacac ctctcgcacc ttttcaacac tcatttgcat
     9121 aagcgaatct tcagtcccca aaatatttga agctaattca tgatcttgaa aaaaacactc
     9181 ttcaaattca tcaaatatta attcaccagg agtatcttta tatgaattta tttcatcaag
     9241 tatcacttca cgctctttaa taaattcttg ctgcaaaaat tgagaattat aaaaaatatc
     9301 atgcaataca tctacggccc tttccaaata ttgattttgt atagtaatgt atatacacgt
     9361 ttcttctttt gacgtatacg cattcatatc gccaccaaca gactcgatta agctcatcaa
     9421 ttcataataa ttccgcgttt gagtaccctt aaataacata tgctctatta catgcattac
     9481 accatcacac cctacgggtt catcacgtga acccgaatgc acaaacaaac atatatacga
     9541 aaccaaagaa tttgattttg aaagcaccac ctttgcttta ttaggtaatt caaaaaacac
     9601 ctctcgctct acagcaacca tagcaaccct gctttatgtt tttgttccac gtggaacatt
     9661 attataattt ttagtaattg tttcacgtgg aacacatttt tatctcccta aatacactaa
     9721 taaaacactt atatcagcag gtgaaacacc gctaatacga gaagcttgac caattgtttc
     9781 cggattaatt tcagttaatt tttgccttgc ctcggttgac aaacttggca tttccatata
     9841 tggaattttt cctttgattt taatatattc taatcgactg attttttcag caatttgttt
     9901 ttctttttca atataccctg aatactttat atcaatatct actacttcaa gcaatgtttc
     9961 tttatctgtt tcacattgat tcaataaatc ttcaggtatt ggaaacatag atgcgtatat
    10021 atttggtcgc gaaattaaag cactcatttt catagattgc tttaaaggag aacttccatg
    10081 ttgttccaaa atttcatttg ctttattcat atgaattgtt gtagacttca cgtaatcctt
    10141 aatagtttct attactttat atttattctc aaacaccttt tttctatagt catctactaa
    10201 tcctataact attcctactg gagtaagacg aacatcggca ttatcttgac gcaataaaat
    10261 tctaaattct gcacgcgaag taaacattct gtatggctca ttaacacctt ttgttattaa
    10321 atcatctatt aatactccta tatatgcttc atcacgtttc aaaacaagcg gttctttatt
    10381 tgtaattgct gcatgagcat ttattccggc tattagacct tgcgcagcgg cttcttcgta
    10441 tcctgttgtg ccattaattt gtccggcaaa aaataaattt ttcaccattt tggtttctaa
    10501 acttgcttgt aattgagtag ggtcaaaata atcatactca atggcatatc cggggcgata
    10561 tatctctata ttactaaaag ccggaattt
//
LOCUS       DAOTOW010000005         9056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_6992_length_9056_cov_3.465282, whole genome shotgun sequence.
ACCESSION   DAOTOW010000005 DAOTOW010000000
VERSION     DAOTOW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6992_length_9056_cov_3.465282"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="P5243_00205"
     CDS             <1..682
                     /locus_tag="P5243_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS17896.1"
                     /translation="IVKLFEDYLKHTYLVFSSTSPKEFIDTLDIVMPDIVISDIGMPD
                     IDGYSICKIIKQKYNIPVILISSNREDTIKQYAYACGANAYIDKPVSKDMLFAVIKNM
                     ESQIFAHGNKNDTVIQDIDDEAVFLKKFEQILVQNLANTSFSVENIAMQMNLSRTQLF
                     RKIKQIHAMSPKEYLNKMRMQTAGELLKAGDAKIVEVAYAVGFSDPSYFTKCFIKFYG
                     VSPSEYKG"
     gene            750..1520
                     /locus_tag="P5243_00210"
     CDS             750..1520
                     /locus_tag="P5243_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS17897.1"
                     /translation="MKIAIHTRFLVKNKLEGIGRFTFETVKRLCENHPEHTFYLLFDR
                     MYEPEYIFSSNVKPIVVPFPARHPFLWWFWFHIQVPRVLKNISADVFVSTDGFLPLNV
                     SIPTVNVIHDLNFEHNPEHVPWLVRWYYKKYFPLYARKATRIATVSEFSKHDIATTYG
                     IDMHTIDVVYNGVSSEFEPLTEVQKTIVKKTYTDGCDYFMYVGSLHPRKNIERLFAAF
                     ELCKKQTQTSCKLVIVGKLCFYQNLFKKHIILMLTKTI"
     gene            1460..1870
                     /locus_tag="P5243_00215"
     CDS             1460..1870
                     /locus_tag="P5243_00215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS17898.1"
                     /translation="MFLSKSIQKAYNSNAYKNDIIFTGRVPSHQLGLIVGAAYAMTYV
                     PLFEGFGIPILEAFACNIPVITSRCTSMPEVAGNAALYCDPLHTESIAVAMKKILNDK
                     ELYQKLLEYAYIQKQKFSWDKTADLVWKSILKVV"
     gene            1843..2058
                     /locus_tag="P5243_00220"
     CDS             1843..2058
                     /locus_tag="P5243_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17899.1"
                     /translation="MEKYIKSSIIFISIIVSCAACKRNNCIEKKSDDCYCTYQYDPVC
                     GCNNKTYGNACVAQCNGITEFTKGECK"
     gene            2060..2962
                     /locus_tag="P5243_00225"
     CDS             2060..2962
                     /locus_tag="P5243_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS17900.1"
                     /translation="MWVVIVIFSAIFLGVYDVFKKLSLTHNAVLPVMLVSSTTSLLFF
                     IPGIVLSAFYPQTVEGTIFYVPFSGAHTQWLIFIKSCIVQASWVFSFFALKHLPISIV
                     SPIRSTGPMWTLVGAIFILGETLTPLQWTGVLLIVACLYAYSLAGKKEGIVFKSNIWV
                     LFIFIGTALGSLSALYDKYLLATLGINKMEVQAWFSVYQCMLMIPTVLLLWFPYRHKT
                     DAFQWRWTIPLIGVFLVLADFFYFYALSLPGALVSVISLIRRSGVIISFLSGVIIFKE
                     QNLRFKGFILAGVLVGVYVLYLAR"
     gene            3034..4695
                     /locus_tag="P5243_00230"
     CDS             3034..4695
                     /locus_tag="P5243_00230"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HRS17901.1"
                     /translation="MKDIIQLFTQESTASTFVIIAIISILGILIGKIPVFKIKLGIAG
                     VLFAGIFVGHLGAHINHDVLHFIKEFGLILFVYSIGIDIGPRFVASFRNNGITVNLLA
                     TSIVVLGFFIAVLLQYIFNLDVEVIAGILSGAVTNTPSLGAAQTVITEQIPNGVEHAP
                     VAGMAYAVAYPFGIIGIILVMFILKYLFKISIQREIESYNKDVTGLSGAMHSVHVEVV
                     NPNVIGKSIEFLRKMVDTEFVISRLKRNNEFIVPANTTIIQQGDIIYGVSTDDKYPKL
                     EVTIGPVHKTGRVEITGNLTMRHVLVTNRAISGKTLQEIGLSEQFPANITRIFRAGTE
                     MLPTSETTLEFGDTVRVVANRDSIDVVAKYLGNSQKSLSHPNLIPLFIGVLLGVILGS
                     IPFFIPGLPAPAKLGLAGGPLIIALLLGHKGRIGSFDFYMTPSANLFIREMGIVLFLA
                     CVGLGSGAHFWETIIQGGYMWMLYGAIIKFVPILIVGIVARMKRINFLTICGILAGAM
                     TDPPALEFANSMAPVQAQATAYATVYPFTMFLRILSAQILVLVLL"
     gene            complement(4690..5733)
                     /locus_tag="P5243_00235"
     CDS             complement(4690..5733)
                     /locus_tag="P5243_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRS17902.1"
                     /translation="MKDRILTILHKIPMPGDTKNIVEAGIISNITTDIHNITIEIGLP
                     QTFEKYKQALIPTIEDALRREISFTGTISIPITILKANHSNEPGGISQVTHIIAIASG
                     KGGVGKSTVSSNLAIALSQLGYSVGLLDADIFGPSIPKMFHIEHGQPTMKKIGDKEYI
                     VPIEQYGIKLLSIGFFVKSSDALAWRGPMAGNVLKQFISDTDWGELDFLLLDMPPGTS
                     DIQLTIAQTIDLTGAIMVSTPQQVALVDVQKGIDFFKKDGIQVPILGIIENMAWFTPA
                     ELPNNKYYIFGKNGVQDLAQRLNIPLLGSVPIVQSICEAGDNGTPITLQEDSIVSIQF
                     REIAQTLTTKLEL"
     gene            complement(5737..6582)
                     /gene="prmC"
                     /locus_tag="P5243_00240"
     CDS             complement(5737..6582)
                     /gene="prmC"
                     /locus_tag="P5243_00240"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRS17903.1"
                     /translation="MTLSDVKIEFHSKLHNIYPKNEISAILGLLLSDIGYSNSDIVIS
                     ANSILTNTQIDYVQTALSKLCNHTPVQYIRQKADFYGLEFFVNPSVLIPRQETEILVH
                     TIIHSNNKKSTIVDIGTGSGCIAITLAKHLPQAQVYAIDISKEALAVTKQNAIHHSVE
                     IQCIEADMCNIDTYSKIPTCTTIVSNPPYICSNEKKYMNLNVLEHEPHTALFVPDNNP
                     LQYYKALANIGLQKLETNGMLICEINEAYGIETKELFEQFGFVNCSIILDLHNKDRFL
                     QAYKL"
     gene            6625..7686
                     /gene="ribD"
                     /locus_tag="P5243_00245"
     CDS             6625..7686
                     /gene="ribD"
                     /locus_tag="P5243_00245"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRS17904.1"
                     /translation="MTENIDKKYMHMCLQLAQQAFGNTYPNPMVGCVIVYNSVVIGQG
                     YHHKAGEPHAEVLAIESVQDKSLLSQSTLYVNLEPCAHYGKTPPCSLAVIQNNIPRVV
                     IGCVDTFSKVAGKGIQMMQNAGIQVEVGILEQESRELNKRFFTYHEQKRPYIILKWAQ
                     TQDGFIDIAPEYKMHKKGLWITDDICKTLVHKWRTQEQAILIGTNTADVDNPQLTARL
                     VQGNQPLRIVIDRQNRLPDNLYVKDGSVSTIIYTEELRNTHKYNVEYVNIDFSFNLWQ
                     QIYNDLYEREIQSVIIEGGAQILEDCIQNNLWDEMRVFIGPISFKKGVLAPNIESLQP
                     QNTEIVGNSVLHVFFNQKI"
     gene            8019..>9056
                     /locus_tag="P5243_00250"
     CDS             8019..>9056
                     /locus_tag="P5243_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRS17905.1"
                     /translation="MKTKVLLLVVCSFAAQLVFAQINSNSPTVPFGSRSSYPNGIMPT
                     NLPTGGTYGKSQAAADAYNAFKTNLLENCSNGIRVKFDDNTSTVSEGIAYGMLLAAYA
                     ADKTTFDGLWKYYLSQKNSNGVMNWKIGGCTGVTGQNGATDAELDAAMALIIASEQWP
                     TATSPYTYKNEALYLIGKIRQYEIHPSTYQTLNGDMWGTTNTCRNPSYFAPAYYREFA
                     KVETAQASFWNSVVSTSNSFLLTNRNATTGLVSNWSDNNATPNTCNGPNEFGWDAIRN
                     PWRMAVDYIWNGATAASTASDICSKMSNWAKNDAANLKGPCAMNAASPASGTYKNGTF
                     GMIGLAFMGTSG"
BASE COUNT         2960 a         1300 c         1655 g         3141 t
ORIGIN      
        1 aattgtaaaa ttgtttgaag attatttaaa acatacatat ttagttttta gcagtactag
       61 tcccaaagaa tttatcgata cacttgatat tgtaatgccc gatattgtaa tttcagatat
      121 cggaatgccc gatatagatg gctatagtat atgtaaaatt attaagcaaa agtataatat
      181 tcctgttatt ttaatttctt caaatagaga agacacaatt aaacaatatg cctatgcatg
      241 tggagccaat gcatacattg ataaacctgt aagtaaagat atgttgtttg ctgttatcaa
      301 aaacatggag tcgcaaatat ttgcacatgg aaataaaaat gatacagtaa tacaagacat
      361 tgatgatgaa gctgtatttc ttaaaaaatt tgagcaaatt ttagtgcaaa atttagcaaa
      421 cacgtctttt tctgttgaaa atattgctat gcaaatgaat ctaagcagaa cccaattatt
      481 tagaaaaatt aagcaaatac atgccatgtc gcccaaagaa tatttaaata aaatgcgtat
      541 gcaaactgcc ggagaactat taaaagcagg agatgctaaa attgttgaag tagcttatgc
      601 tgtgggtttt tctgatcctt cttattttac taaatgtttt attaaatttt atggggtttc
      661 gccaagtgaa tacaagggct aatatttcag ttattttttg catattacaa aataattttt
      721 tagttacttt gtaatataaa aatataaaaa tgaaaatagc catacataca cgttttttgg
      781 ttaaaaataa gcttgaaggt ataggtagat ttacatttga aacggtaaag cgattatgcg
      841 aaaatcatcc tgaacataca ttttatttgc tatttgaccg aatgtatgaa cccgaatata
      901 ttttttcatc aaatgtaaaa cctattgttg ttccgtttcc tgcacgtcat ccttttttat
      961 ggtggttttg gtttcatatt caagtgccgc gagtgcttaa aaatatttct gctgatgtgt
     1021 ttgtatcaac tgatggtttt ttgccgctta atgtatcaat tcctacagta aatgtaattc
     1081 acgatttgaa ttttgaacat aatcccgaac atgtaccatg gttggtacga tggtattata
     1141 aaaaatattt tccattgtat gcccgtaagg caacacgtat agctactgtt tcggaatttt
     1201 caaagcatga tattgccact acttatggaa tagatatgca tacaatagat gttgtttata
     1261 atggagtaag cagcgagttt gagcctctta ccgaagtgca aaaaacaatt gtaaaaaaaa
     1321 catatacaga cggatgtgat tattttatgt atgtagggtc tttacatccc cgaaaaaata
     1381 tagaacgttt gtttgctgcc tttgaattat gcaaaaaaca aactcaaaca tcttgtaaat
     1441 tagttatagt aggtaagcta tgtttttatc aaaatctatt caaaaagcat ataattctaa
     1501 tgcttacaaa aacgatataa tttttaccgg cagagttccc tcacatcaat tggggctaat
     1561 agtaggagca gcatatgcca tgacgtatgt tcctttattc gaaggatttg gaattcctat
     1621 actcgaggct tttgcatgta atattcctgt tattacatcg cgttgtactt caatgcccga
     1681 agttgccggt aatgctgcac tgtattgcga tccgttgcat acagaatcta tagcagttgc
     1741 aatgaaaaaa atattgaacg ataaggaatt gtatcaaaaa ttacttgaat atgcatatat
     1801 acaaaaacaa aaattttcgt gggataaaac agctgattta gtatggaaaa gtatattaaa
     1861 agtagtataa tttttataag tatcattgtt agttgtgcgg catgcaaacg taataattgt
     1921 atcgaaaaaa aatcggacga ttgttattgt acatatcaat acgacccggt atgcggatgt
     1981 aataataaga cctatgggaa tgcttgtgtt gctcagtgta atggcataac agaatttact
     2041 aaaggagaat gcaaataata tgtgggtagt tattgtaatt ttttctgcaa tttttttagg
     2101 ggtgtacgat gtttttaaaa aactatcatt aacacacaat gcggtattac ctgttatgct
     2161 tgtttcatca actacctcat tgttgttttt tattcctggt attgtattgt ctgcattcta
     2221 tcctcaaact gtagagggaa caatatttta tgtgccattt tcaggagctc atacgcaatg
     2281 gttaattttt ataaaaagtt gtatagtaca ggcatcatgg gtgttttctt tttttgcact
     2341 taaacattta cctatttcca tagtaagccc tatacgttct acaggtccta tgtggacact
     2401 ggttggggca atatttattt taggtgaaac tttgacgccc ttacagtgga caggtgtttt
     2461 actcattgta gcatgtttat atgcctattc tttggcaggc aaaaaagaag gtatagtgtt
     2521 taaaagtaat atatgggtat tgtttatatt tataggaacg gctttaggat cacttagtgc
     2581 attgtacgat aagtatttat tagctacatt aggcatcaat aaaatggaag tacaagcgtg
     2641 gttttcggta tatcaatgta tgctcatgat tcctacagta ttgttattat ggttccctta
     2701 cagacataaa actgatgcgt ttcaatggcg gtggactatt cctttaatag gtgttttttt
     2761 ggtattagca gatttctttt atttttatgc attaagttta ccgggagcac ttgtttcggt
     2821 tatatctctt attcgtagaa gtggtgttat tatatctttt ttgtcgggag ttattatttt
     2881 taaagagcaa aatcttagat ttaaaggatt tatattggcc ggagtattgg tgggtgtata
     2941 tgttttgtat ttggctcgtt aaattaatat caaaaaatca aattttttta attgaaatct
     3001 atacatttgt tgtgtacaaa aacagttgaa accatgaaag atattataca actatttaca
     3061 caagaaagta cagcgtcaac gtttgtaatc attgctatta tcagtatttt aggtatatta
     3121 ataggtaaaa taccggtatt taaaattaag ctgggtattg caggggttct ttttgccgga
     3181 atatttgttg gtcatttagg agctcatatc aatcatgatg tgttacattt tataaaagaa
     3241 tttggactta ttctttttgt atattctata ggtattgata taggtccacg ctttgtagct
     3301 tcattcagaa acaatggtat tactgttaat ttactagcta catctattgt tgtattaggt
     3361 tttttcattg ctgttttgtt acagtacatt tttaatttag atgttgaagt tattgcagga
     3421 attttaagtg gtgcagttac aaatactcca agtttgggag cagcacaaac agttataaca
     3481 gaacaaatac ccaatggtgt agaacatgca cccgttgccg gaatggctta tgctgttgca
     3541 tatccgtttg gaataatagg aattatattg gttatgttta ttctcaaata tctttttaag
     3601 atttcaattc aacgcgaaat agaatcgtac aataaagatg ttacaggatt aagtggagct
     3661 atgcattcgg tacatgtaga agttgtaaat ccaaatgtta taggtaaatc aattgaattt
     3721 cttcgtaaaa tggttgatac ggaatttgta atttctcgtt taaaacgtaa taatgaattt
     3781 atagtgcctg ccaatactac aattatacaa caaggtgata ttatatatgg tgtttcaacc
     3841 gatgataaat atcccaaatt ggaagttacc attggacctg tacataaaac aggtagggta
     3901 gaaataacag gaaatttaac aatgcgacat gtgttggtta ctaatagagc tatttctggt
     3961 aaaacgttgc aggaaattgg attatcggaa caattcccgg ctaatattac ccgaattttt
     4021 agagcaggaa cagaaatgtt gcctacaagc gaaacaacat tagagtttgg tgacactgta
     4081 cgtgtggttg caaatagaga ttctatagat gtagttgcaa aatatttagg gaattcacaa
     4141 aaatcattgt cacaccctaa tttaatacca ttgtttatag gtgttttact tggtgtaatt
     4201 ttaggaagta ttccattttt tataccagga ctacctgctc cggctaaatt aggattagcg
     4261 ggtgggccac ttatcattgc cttgttatta ggccacaaag gaagaatagg ttcttttgat
     4321 ttttacatga caccaagtgc aaatttattt atccgtgaaa tgggtatagt attatttttg
     4381 gcatgtgttg gtttaggttc gggggcacat ttttgggaaa caattataca aggtgggtat
     4441 atgtggatgc tatacggagc tattattaaa tttgtgccta ttttaatagt aggtattgta
     4501 gcacgaatga aacgcattaa ttttttaact atatgcggta tattggcggg tgcaatgact
     4561 gatcctccgg cacttgaatt tgctaattct atggcacctg tacaagctca agctacagcg
     4621 tatgctactg tgtatccttt tactatgttt ttgcgaattt tatcagctca aattttggtt
     4681 ttagtattat tataattcca attttgtagt tagtgtttga gcaatttctc tgaattgtat
     4741 tgatacaata gaatcttctt ggagtgtaat tggagttcca ttgtcgccgg cttcgcatat
     4801 gctttgaact ataggaacag aacctaacaa aggaatattt aatctttgag ctaaatcttg
     4861 aactccgttt ttgccaaaaa tgtaatactt attatttggt aattcagcag gggtaaacca
     4921 agccatattt tctataatac ccaaaattgg aacttgtata ccgtcttttt taaagaaatc
     4981 aatgcctttc tgaacatcta ctaaagcaac ttgctgtggg gtgctaacca taattgcacc
     5041 tgttaaatct attgtttgtg ctatagtgag ttgaatatca ctcgttcccg gaggcatatc
     5101 aagtaataaa aagtccaatt ctccccagtc tgtatcagaa ataaattgtt ttaaaacatt
     5161 tccagccata gggccccgcc aagctaaagc atcggaactt tttacaaaaa aacctattga
     5221 caatagtttt ataccatatt gttctattgg tacaatatat tctttatcac ctattttctt
     5281 catggtaggt tggccgtgtt ctatatgaaa catttttggt atcgaaggcc caaaaatatc
     5341 tgcatcgagt aaccctacgc tatatccaag ttgtgataat gctattgcta aattagagct
     5401 tactgttgat tttcctacac cacctttacc cgacgcaata gctataatgt gtgtaacctg
     5461 tgaaattcca ccgggttcat tgctatggtt tgctttgagt atggtaatgg gaattgaaat
     5521 agttcccgta aaagagattt ctctacgaag agcatcttca atagtaggaa ttagggcttg
     5581 cttatatttt tcaaaggttt gcggtaatcc tatttctata gtaatattgt gtatatcagt
     5641 tgtaatattt gaaattattc cggcttctac aatatttttt gtatcgcccg gcataggaat
     5701 tttgtgtaaa attgtaagta ttctatcttt cataatttaa agtttatatg cttgtaaaaa
     5761 tctatcttta ttgtgtaagt cgagtataat tgaacaattt acaaaaccaa attgttcaaa
     5821 taattcttta gtttcaatac cataagcttc gttgatttcg caaattaaca tgccgtttgt
     5881 ttcaagtttt tgtaatccta tattagctaa tgctttataa tattgtaacg gattgttgtc
     5941 ggggacaaac aatgctgtat gaggttcatg ctctaataca tttaaattca tgtacttttt
     6001 ttcattagag catatatagg gtggattact tacaatagtt gtgcatgttg gtatttttga
     6061 atatgtatca atattgcaca tatctgcttc tatacactgt atctcaaccg aatggtgaat
     6121 tgcattttgt tttgttactg caagtgcttc tttagaaata tcaatagcgt aaacctgtgc
     6181 ttgaggtaaa tgcttggcaa gagtaatagc aatgcatcca ctgcctgtgc caatatcaac
     6241 tatagtagat tttttgttat tggaatgaat aattgtatgt acaagtattt cggtttcttg
     6301 tcggggtatt aataccgagg gatttacaaa aaattctaat ccgtaaaaat cagctttttg
     6361 tcgaatgtat tgaaccggag tatgattaca gagtttagac aatgctgttt gtacatagtc
     6421 tatttgggta tttgtaagaa ttgaattggc agaaattaca atatcagagt tagaataccc
     6481 tatatcagaa agtaataatc ctaaaattgc tgaaatttca tttttaggat atatgttgtg
     6541 taatttcgaa tgaaattcta tttttacatc agataatgtc attgacctac atttttaagt
     6601 caaaagtaca caattagaat ttacatgaca gaaaatatcg acaaaaaata tatgcatatg
     6661 tgtttgcagc ttgcacagca agcatttggt aacacatatc ctaatcctat ggtggggtgt
     6721 gtaattgtat ataattctgt tgtcataggt cagggatatc accataaagc gggagagcct
     6781 catgcagaag ttttggctat agaatcagta caagacaaat cgctgttgag ccaatctaca
     6841 ttatatgtta atttagaacc ttgtgctcat tacggaaaaa caccaccgtg ttcacttgct
     6901 gtaatacaaa ataatatacc acgagtagta ataggttgtg tagatacatt tagtaaagtt
     6961 gcaggtaaag gtattcaaat gatgcaaaat gcaggtattc aagttgaggt tggaattctt
     7021 gagcaagaat cacgagaatt gaacaagcgt ttttttacgt atcatgaaca aaagcgaccg
     7081 tatataattt tgaaatgggc acaaacgcaa gatggcttta ttgatatagc tcccgaatat
     7141 aaaatgcaca aaaaaggttt gtggataact gatgatattt gtaaaacttt agtccacaaa
     7201 tggagaaccc aagagcaagc tattttaatt ggaactaata ctgcagatgt agataatccg
     7261 caacttactg cccgattggt gcaagggaat caaccacttc gtattgtaat tgacagacaa
     7321 aacagattac ccgataattt atatgtaaaa gacggttctg tatctacaat tatttataca
     7381 gaggaattac gtaatactca taaatacaat gtagagtatg tcaatattga tttttcgttt
     7441 aatctatggc aacagatata taatgattta tatgaaagag aaattcaatc tgttataata
     7501 gaaggagggg ctcaaatttt agaagattgt attcaaaata atctatggga cgaaatgcgt
     7561 gtttttattg gtccaatttc ttttaaaaaa ggagttttgg caccaaatat agaatcatta
     7621 caaccacaaa ataccgaaat tgtagggaat tcagttttac atgttttttt taatcagaag
     7681 atttaaaaat aatttattgt atagaataaa ttttgagcat aataatataa aaatacgtat
     7741 taatgcgtaa tgaataattt atgttttttt aaaaaactaa taggttcaac ttgaaaattt
     7801 attcaaatta tagtaaaaaa ttatccaata actacttttt taatcaaaaa tatacatgta
     7861 aatatttgat tttcaataaa taattattat actgtacaat agataccaac tatatagggt
     7921 atttataaaa aaaagctgtc ttttgttatg gaaaaatatc gtatttttac acgattttct
     7981 ctgaatgggg ttttattaaa ttttttaaaa aaaaagttat gaaaacaaaa gtgcttttat
     8041 tagtagtatg tagttttgca gcacagttgg tgtttgcaca gattaactca aacagtccaa
     8101 cggttccgtt tggttcacgt tcgagttatc cgaatggtat tatgccaacg aatttaccta
     8161 ccggtggaac ctatggtaaa tcgcaagctg ctgccgatgc atataatgcg ttcaaaacaa
     8221 atttattaga aaactgtagt aatggtattc gtgtaaaatt tgatgataat acatcaacag
     8281 tttctgaagg tattgcatat ggtatgttgt tagctgcgta tgctgccgat aaaacaacat
     8341 tcgatggatt atggaaatat tatctatcac aaaaaaatag taatggtgta atgaattgga
     8401 aaattggcgg atgtacggga gtaaccggtc aaaatggagc aaccgatgcc gagcttgatg
     8461 ctgctatggc gttaattata gcttcggaac aatggccaac tgcaacaagt ccatatacat
     8521 acaaaaatga agctctttat ttaataggaa aaattcgtca atatgagatt catccatcaa
     8581 catatcaaac attgaatggt gatatgtggg ggacaacaaa tacgtgtaga aaccctagtt
     8641 attttgctcc tgcttattat agagaatttg caaaagtaga aactgcacaa gcaagttttt
     8701 ggaatagtgt tgtttcaact tcaaatagct ttttgttaac aaacagaaat gcaacaaccg
     8761 gtttagtaag taactggtca gataataatg caacaccaaa tacttgtaac ggaccaaacg
     8821 aatttggttg ggatgctata cgtaacccat ggcgtatggc tgtagactat atttggaatg
     8881 gagcaactgc agcatctact gcgtctgata tttgtagtaa aatgtcaaat tgggcaaaaa
     8941 atgatgctgc aaatttaaaa ggtccgtgtg ctatgaatgc tgcaagtcct gcgagtggaa
     9001 catataaaaa tggaacattt ggtatgatag gtcttgcttt tatgggaacc tcggga
//
LOCUS       DAOTOW010000006         8633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_7390_length_8633_cov_3.308114, whole genome shotgun sequence.
ACCESSION   DAOTOW010000006 DAOTOW010000000
VERSION     DAOTOW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_8633_cov_3.308114"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1348)
                     /gene="metK"
                     /locus_tag="P5243_00255"
     CDS             complement(68..1348)
                     /gene="metK"
                     /locus_tag="P5243_00255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRS17906.1"
                     /translation="MGYFFTSESVSEGHPDKVSDQISDALLDAFLAQDPNSKVACETL
                     VTTGLTVLSGEVKSKAYVDVQQVARDVINQIGYNKAAYKFDGDSCGVISAIHEQSADI
                     NQGVERENPYDQGAGDQGMMFGYAVDETSTYMPLTLDISHRLLERLAYVRKNMKVMPY
                     LRPDSKSQVTIEYDSKTNKPVRIDTIVISTQHDDFAKTDKEMQDKIAADLRKYVIPQT
                     RNMYPAKMRKLFDTEFKLLVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGHGAHGGGAF
                     SGKDSSKVDRSAAYAARHIAKNIVAAGVAHQCLVQVSYAIGVAEPVSIYINTYGTCKA
                     KKSNGHKFTDEEIATIVFEMFDLRPAAIVERFGLKNPIFLPTASYGHFGRNPYTQAVT
                     VFENGVEKIKEVQFFGWECLDYVDKLREGFGLLK"
     gene            complement(1525..3138)
                     /locus_tag="P5243_00260"
     CDS             complement(1525..3138)
                     /locus_tag="P5243_00260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRS17907.1"
                     /translation="MKPIQLLLSRPWLYLVIILIGIGFKWYAIDYKMMWYDEIATVLQ
                     IQGADNFFDVDSTQLNTIVPISHYKGILQYSNKPQITFVSEIQAQIHNMNLNPLHYML
                     LSVWYRIFGESLVWYRLFSVFVFLLSLPFIYGIAKELFRSQAAGLIAVALFSVSPFIH
                     YFAQELRYYMLWAFLLIALHYFLLKSVLYNKNQWWIGYGICAVLSLYASTLSGFIIFE
                     HALIVFMFFKHARIRFFVVILFVIVLYAPWFLYVYSNKQEVYQSLSWHKFEGLPLWAP
                     LLGLVLGLVRTFSFYLQYTLFWDDVLSAITTAMIVETIFNIIILIFIIVSAKRLIQYE
                     SPKTAWFVLLILIPGLVFFWGLDIVRNAITTHWWRYYIFNTIPVILLVTYGIYSYMHK
                     SKGIAFLFIIGLSSVSIYSMYAISQEKYWYLGGNWQQEFVDNAQTLTQATKPLVISDF
                     IRLQNPWEGPMHTMEVLVNCNSENIDVLRLSANKENIKSMIPLHTYTDIFVMYASDEL
                     LQNLQNQFGNSLKIIERKQGPPLWKIELE"
     gene            complement(3161..4069)
                     /locus_tag="P5243_00265"
     CDS             complement(3161..4069)
                     /locus_tag="P5243_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS17908.1"
                     /translation="MKVSIIICTYNRESYILDSLMSLKDQSSNYYNYEIIIIDNNSTD
                     STPLLCMEFLQQKGDLNVHYYKETQQGLSYARNRGIAVAQGEIIAFIDDDAVACRDYV
                     LNIIEWFDNHSECAAIGGRIFPKYETQCPTWMPEQLMPLFSILNLGEKPKEFTRNLYP
                     VGANMAFRKSVFTQYGLFKTELGRTGKNLLGGEEKDIFFRLKKHKLAIWYVPYIWVYH
                     IIPQQRTTKDFIMKQAIGVGQSEYFRSKSNIVAYSSSICKELIKWGVSKLLALGYTIQ
                     AKPQKAAMILQFRYYVSLGLLGIKRV"
     gene            4209..>5026
                     /locus_tag="P5243_00270"
     CDS             4209..>5026
                     /locus_tag="P5243_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17909.1"
                     /translation="MKKTTFLFIALCSTYIGFSQANDTVINKWRNGKISSKGYMLDGT
                     EQGTWQYWDSLGNKVEETNFLRGRLHGKSTIYYTNGKKHIESNYKLNQLHGMYTEWDE
                     AGYIKVQGYYKNHQRDSSWKYFYPNTNLMQYEYIYKTKDTVLLWNYCYRRGTCTVRNG
                     NGTYEEYWNESSNLKEKGAYKDGFKDGYWEMYFQDGKLKGKGSYVRGEKVGNWIEYYA
                     NGTLKERIDHTKGTYETWHPNGKKYIVGYLKNDLKDSVWQYWNADGVLITRARYV"
     assembly_gap    5027..5036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5037..5942
                     /locus_tag="P5243_00275"
     CDS             <5037..5942
                     /locus_tag="P5243_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17910.1"
                     /translation="ENWFANGKPSSTFEYKNNQKNGKAVWWHETGEKDMEGYFQNDLQ
                     HGDWIYWRKDGGIGNTGKYIQGKMNGHWKWFYANGAVWKEGDYIDDLKTGVWIFYYEN
                     GQKEHQGTFVNDKENGLFEQWFENGMIKTRGYFDMGKMTGTWEGWFEENPKQQKYSNQ
                     YKNDLRHGTSKYWNEAGLLQSEKNYANGELHGTFKTYFPDGKINISGEYDKGKMTGTW
                     QYYNQSGTCERMMIYKDGLPNGKWEVRYKEGPLKEQTQYKDGVRHGKTKMYALKGDVL
                     YYAKYKNGELKKVKIDNRQKTPGVK"
     gene            5958..6698
                     /gene="kdsB"
                     /locus_tag="P5243_00280"
     CDS             5958..6698
                     /gene="kdsB"
                     /locus_tag="P5243_00280"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRS17911.1"
                     /translation="MKALVIIPARYGSTRFPGKPLAIIHGKSMIQRVYEQAQQVVPDV
                     WVATDDARIEHEVKRFGGKAIITSVEHQSGTDRLAEALSSIPGALKYSVVVNVQGDEP
                     FIAAEQIQQLIQLFKQPDTQIATLIKPITNTEDLMNPNKPKVVIGTNMQALYFSRSPI
                     PFVRDADKIYWHIAHQYYKHIGIYAYRPQILLDITKLERSSLEIAENLEQLRWLQNNY
                     VIKTAITEYESASVDTPDDLEKILNKRF"
     gene            6767..8017
                     /locus_tag="P5243_00285"
     CDS             6767..8017
                     /locus_tag="P5243_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS17912.1"
                     /translation="MNVQQIKQRFGIIGNTTRLNRAIETALQIAPTDLSVLIVGESGT
                     GKEVFSQIIHQNSARKHNQYIAINCGAIPDGTIDSELFGHEKGAFTGAVDSRKGYFEV
                     VDGGTIFLDEVGELPLNTQVRLLRILEVGEYIRVGSSKIQKTNVRVVAATNVNLLKAV
                     EEGKFREDLYYRLNTVPITIPPLRERKEDIPLLFKKFANDSSEKYGIPPLRLDQAATD
                     LLISYNWPGNIRQLKNLAEQMSILEKERNITFSVLKSYIPQNYDTRLPAIVPKDSEQA
                     NYASEREILFKLLFDMKKDMNELKKLVHDIINGNQHNIHISTEQAQNLHKIFETEMPV
                     FDAQQNQSYEVSKPFISTVRTDENIIDTHEVVEESLSLVSKEIDFIKRALAKHKGKRK
                     LAAQELGISERTLYRKIKEYNIEE"
     gene            8007..8510
                     /locus_tag="P5243_00290"
     CDS             8007..8510
                     /locus_tag="P5243_00290"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRS17913.1"
                     /translation="MKNNIIISICCILLVASCSVKYSFSGASIAPEIKTFSVAYIENQ
                     APTKQATLSDVFTEGLKEKIRTNTRLSMVNNYGDILFEGAIVDYSVNYLGVTASQLAA
                     SNRLTITVKITCTNTKEPKKSFEKQFSRYYDFDAMQNISTIETAAIKQISDQIIEDIF
                     LEAFANW"
BASE COUNT         3241 a         1434 c         1359 g         2589 t
ORIGIN      
        1 ttttattaaa ttaattttgt agttttatcg gacaataatg atttattata tattatacta
       61 tttaaaatta ttttaacaaa ccaaaacctt cacgaagttt atcaacgtag tctaaacatt
      121 cccaaccaaa gaactgaact tctttaattt tttcaactcc attttcaaaa acagtaacag
      181 cttgagtata cggattacgt ccgaaatgtc cgtacgatgc cgtaggtaaa aagattggat
      241 tttttaaacc aaatcgttca acaatagcag ccggacgtaa atcaaacatc tcaaaaacta
      301 tagttgcaat ttcttcatcg gtaaatttgt gaccgtttga tttttttgct ttacacgttc
      361 catatgtatt tatataaata cttaccggtt ctgcaacacc aatagcatat gatacttgca
      421 ccaaacattg atgagcaacc cctgcagcaa caatattttt agcaatatga cgagcagcat
      481 aagcagcact acggtcaact tttgatgaat cttttccgga aaaagctcca ccaccatgag
      541 caccatgtcc gccataagta tcaactataa tttttctacc ggttaatcct gtatcaccat
      601 gtggtccacc aattacaaat ttaccggttg gatttactaa taatttaaac tctgtatcaa
      661 acaatttacg cattttagcg ggatacatat ttctagtttg cggtattaca tattttcgta
      721 agtcagcagc aattttatct tgcatttctt tatcagtttt tgcaaaatca tcgtgctgag
      781 tcgatatcac aatagtatct attcgtaccg gtttattagt ttttgaatca tattctatag
      841 ttacctgcga ttttgaatct ggtcgtaaat atggcataac tttcatgttt ttacgcacat
      901 acgccaatcg ctccaacaaa cgatgagata tatctaaagt aagaggcata taagtagagg
      961 tttcatctac tgcatatcca aacatcatac cttggtctcc tgcaccttgg tcataaggat
     1021 tttcacgttc tacaccttga tttatgtctg ctgattgttc atgtattgct gatattactc
     1081 cacaagaatc tccatcaaat ttataggcag ctttattata tcctatttga tttataacat
     1141 cacgagctac ttgttgcaca tcaacataag cctttgactt aacctcacca ctcaatactg
     1201 ttaaacctgt tgtaactaaa gtttcacatg caacttttga attaggatct tgtgctaaaa
     1261 aagcatctaa taatgcatct gaaatttgat cggatacttt atccggatgt ccttccgaaa
     1321 ctgattcgga tgtaaaaaaa taacccatat actatgtttt taataattaa tcactattag
     1381 tagaaaaatt ggggggaatg cttacacagt aaatgtactg ttggtttagc atttttttac
     1441 cgtggttgca atcaactcaa atctttccac aattttagaa tacaaatata aagtatttta
     1501 ttataaaaca aactattaaa aaaattattc caactcaatc ttccataaag ggggaccttg
     1561 cttacgttca ataatcttga gagaattacc aaattgattt tgtaaatttt gcaataattc
     1621 atcagaagca tacattacaa aaatatctgt atatgtatgt aaaggtatca tacttttaat
     1681 attttcttta ttagcactaa gccttaacac atcaatattt tcggaattac aatttaccaa
     1741 cacttccatt gtatgcatag gtccttccca aggattttgc aaacgtataa aatcagaaat
     1801 taccaatggt ttcgttgctt gtgtaagggt ttgtgcgtta tctacaaatt cttgttgcca
     1861 attaccacca aggtaccaat atttttcttg agaaattgca tacatagagt aaatactaac
     1921 agaactaaga cctattataa ataaaaatgc aattcccttt gacttatgca tatacgaata
     1981 aattccgtaa gtgactagta aaataacagg gattgtattg aatatataat atcgccacca
     2041 gtgggtggta atagcatttc taacaatatc taaaccccag aaaaatacta atccgggtat
     2101 tagaataagc aaaacaaacc atgcggtttt aggtgactca tattgtatca atcgtttggc
     2161 agatactatt ataaatataa gtattattat attaaaaatt gtttctacaa tcatggctgt
     2221 agtgatagca ctaagaacat cgtcccagaa taatgtatat tgcagataaa atgaaaatgt
     2281 gcgaaccaaa cctaacacca aacccaacaa tggtgcccac agaggcaatc cttcaaattt
     2341 gtgccacgat aatgattgat acacttcttg tttgttgcta tatacataaa gaaaccacgg
     2401 agcatataat actattacaa aaagaattac aacaaaaaat ctaattcgag cgtgtttaaa
     2461 aaacatgaaa actataagcg catgttcaaa tataataaaa cccgaaagag tagatgcata
     2521 caatgaaaga acagcacata ttccatatcc tatccaccat tgatttttat tatataatac
     2581 tgatttgagt aaaaaataat gaagtgcaat aagcagaaaa gcccacaaca tataatatcg
     2641 gagttcttgt gcaaaataat gaataaaagg agaaacacta aataatgcaa cagctataag
     2701 acctgctgct tgtgaacgaa aaagttcttt ggctataccg tatataaaag gtaacgaaag
     2761 taaaaataca aaaacactaa acaatctata ccataccaat gattcaccaa atatacgata
     2821 ccaaacggat aacagcatat aatgcaaagg atttaaattc atattatgaa tttgagcttg
     2881 tatttcactg acaaacgtta tttgaggctt attactatac tgtaaaatac ctttataatg
     2941 agatattggt actattgtat ttaattgagt actatctaca tcaaaaaaat tgtctgcacc
     3001 ttgaatttgc agaaccgtag caatttcatc ataccacatc attttgtaat caatggcata
     3061 ccatttaaat cctataccaa taagaattat aactaaataa agccatggtc gcgaaagtag
     3121 taattggatt ggtttcatta caataataat tataaattga ttacactctt ttaattccca
     3181 acaaacctaa tgatacataa tatctaaact gtaaaatcat ggccgctttc tgaggttttg
     3241 cttgaatagt ataccctaat gcaagaagtt ttgaaactcc ccattttata agttctttac
     3301 aaatactact gctataggca acaatatttg atttacttct aaaatattca ctctgcccta
     3361 cccctattgc ttgcttcatt ataaaatctt tagtagttct ttgttgcgga attatatgat
     3421 atacccaaat ataaggaaca taccaaattg ctaatttatg tttctttaat ctaaaaaata
     3481 tatctttttc ttctcctccc aataaattct tgccagttct gccaagttct gttttaaaca
     3541 aaccatattg agtaaatacg cttttacgaa atgccatatt tgcacctaca ggatataaat
     3601 tacgtgtaaa ttcttttggt ttttcaccca aattaagaat cgaaaataat ggcattaatt
     3661 gctcaggcat ccatgtggga cactgtgtct catattttgg aaatattcta ccacctatag
     3721 ctgcacattc actatgatta tcaaaccatt ctataatatt caacacataa tctctgcaag
     3781 ctacggcatc atcatcaata aaagcaatta tttctccttg agcaactgct attcctctat
     3841 tacgagcata tgaaagtcct tgttgagttt ctttataata atgtacattc aaatctcctt
     3901 tttgctgtaa aaattccata cacaacagtg gcgtagaatc tgtagaatta ttatcaatta
     3961 taataatttc gtaattataa tagttacttg attgatcttt aagactcata agagaatcaa
     4021 gaatatatga ttctctgttg taagtacaaa ttataattga aactttcata gcatattttt
     4081 atttcaaaaa tacatttttt tacctcatgt attgtaatat aatatatagt tttacaaatt
     4141 attaaaattt gtagagtcga atttggcagt ttttgtattt ttgtacgaat tttaattttt
     4201 ctttagctat gaaaaaaact acattccttt ttattgcatt atgttctacg tatataggtt
     4261 tttcgcaggc aaatgacacc gtaataaaca aatggcgaaa tggaaaaata agcagcaaag
     4321 ggtatatgct tgatggaaca gaacaaggca catggcaata ttgggattca ctgggcaaca
     4381 aagttgaaga aacaaatttt ttacgaggaa gactccatgg taaaagtaca atttactata
     4441 caaatggcaa aaaacatata gaatcaaatt ataaactgaa ccaattacat ggaatgtaca
     4501 ccgaatggga cgaagccgga tatataaaag tacaaggata ttataaaaac catcaacggg
     4561 atagttcatg gaaatatttt taccccaaca ccaatttaat gcagtatgaa tatatataca
     4621 aaaccaaaga tactgtattg ttatggaatt attgctatag acgtggtacg tgtacagtta
     4681 gaaatggtaa tggaacatat gaagaatatt ggaatgagtc atcaaattta aaagaaaaag
     4741 gggcatacaa agacggattc aaagacggat actgggaaat gtattttcaa gatggcaaac
     4801 ttaaaggaaa agggtcctat gtacgtggtg aaaaagttgg taattggata gaatattatg
     4861 ctaacggtac cttaaaagaa cgaatagacc acacaaaagg aacatacgaa acatggcatc
     4921 cgaacgggaa aaaatatata gtaggatatt taaaaaatga tttgaaagat tctgtgtggc
     4981 agtattggaa tgccgacgga gtattaatta cacgagcacg atatgtnnnn nnnnnngaaa
     5041 attggtttgc caacggaaaa ccgtcgtcta catttgaata taaaaacaat caaaaaaacg
     5101 gaaaagcagt atggtggcac gaaaccggcg aaaaagatat ggaaggatac tttcaaaatg
     5161 acttacaaca tggagactgg atatattggc gcaaagatgg tggaataggc aataccggta
     5221 aatacataca aggcaaaatg aatgggcact ggaaatggtt ttatgcgaat ggtgctgtat
     5281 ggaaagaagg tgattatata gatgatttaa aaaccggtgt atggattttc tattatgaaa
     5341 acggacaaaa agaacatcaa ggaacttttg taaacgataa agaaaacggt ttgtttgaac
     5401 agtggtttga aaatggcatg attaaaactc gtggatattt tgatatgggt aaaatgacag
     5461 gaacttggga aggttggttc gaagagaatc caaaacaaca aaaatattct aaccaataca
     5521 aaaatgattt gcgccatgga acatcaaaat attggaatga agcaggacta cttcaatcag
     5581 aaaaaaacta tgcaaatgga gaattacacg gcacatttaa aacatacttt cctgatggta
     5641 aaataaatat tagcggagaa tatgacaaag gaaaaatgac cggcacatgg caatattaca
     5701 atcaatcggg aacgtgcgaa cgaatgatga tatataaaga cgggttacct aacggaaaat
     5761 gggaagttag atacaaagaa ggtcctctca aagagcaaac acaatacaaa gatggagtac
     5821 gacatggaaa aaccaagatg tatgctctaa aaggtgacgt gttatactat gcaaaatata
     5881 aaaatggaga attaaaaaag gtgaaaatcg ataaccgaca aaaaactccg ggtgttaaat
     5941 aatttacata aacaataatg aaagctttag ttataattcc cgctcgttac gggtctacaa
     6001 gattccccgg taaaccatta gcaattatac acggtaaatc aatgattcaa cgtgtatatg
     6061 aacaagctca acaagtagtg cccgatgtat gggttgcaac agatgatgca cgcatagaac
     6121 atgaagtaaa acgttttgga ggaaaagcta taataacttc agtagaacac caaagcggta
     6181 ccgatagact tgctgaagca ctctcttcta ttcccggtgc attaaaatat tcagttgtag
     6241 taaacgtaca aggcgatgaa ccgtttatag cggcagaaca aattcaacaa ttaattcaac
     6301 tcttcaaaca acctgacaca caaattgcaa cccttattaa accaattaca aataccgaag
     6361 atttaatgaa tcctaacaaa ccaaaagttg taattggaac aaatatgcaa gcattatatt
     6421 ttagtcgtag tcctattcca tttgttcgtg atgcagacaa aatatattgg catatagctc
     6481 accagtacta caaacatatt ggaatatatg cttacaggcc tcaaattctt ttagatatta
     6541 ccaaattaga acgctcttca ttagaaattg cagaaaattt agaacaatta cgatggttac
     6601 aaaataacta tgtgataaaa acagcaatta cagaatatga aagcgcatcg gttgatacgc
     6661 ctgatgattt agaaaaaata ctgaataaac gcttttaaag actaattaca attcatacta
     6721 ttgtacaaca catgaaatgt tgtatttttg acaattaaaa aaaaacatga acgttcaaca
     6781 gataaaacaa cggtttggga taattggcaa tacaacacgc ctcaacagag caattgaaac
     6841 tgccttgcaa attgcaccta ccgatttgtc tgtacttata gttggtgaaa gtggcacagg
     6901 gaaagaagtt ttttcacaaa taattcatca aaatagtgct cgcaaacaca accaatatat
     6961 agccataaac tgtggtgcaa ttcccgacgg aacaattgat tccgagttat ttggtcatga
     7021 aaaaggagcc tttaccggcg ctgttgacag tagaaaagga tattttgaag tagttgacgg
     7081 aggaacaatt tttttagacg aagtcggtga attacctttg aatacacaag ttcgtcttct
     7141 tagaatttta gaagtaggag aatatatacg cgtaggttcg tcaaaaattc aaaaaacaaa
     7201 tgtaagagta gttgctgcta caaatgtaaa cttacttaaa gctgtagaag aaggtaaatt
     7261 cagagaagat ttatattatc gtctcaatac ggttcctatt actattcctc cattacgaga
     7321 aagaaaagaa gatatacccc ttctttttaa aaaatttgca aacgacagtt cagaaaaata
     7381 tggcattcca ccattacgac ttgatcaagc tgctaccgat ttattaattt cgtataattg
     7441 gccgggaaat atccgacaac taaagaatct tgcagaacaa atgtcaattt tagaaaaaga
     7501 acgaaatatt accttttcag ttcttaaatc ttacattccg caaaactatg atacccgatt
     7561 acctgccata gttccaaaag attcggaaca agcaaattat gcttcggaac gtgaaatctt
     7621 gttcaaatta ttgttcgaca tgaaaaaaga tatgaacgaa ctcaaaaaat tagttcacga
     7681 tataataaat ggcaatcaac acaatataca tataagcaca gaacaagcac aaaatttaca
     7741 taaaatattt gaaacagaaa tgccggtatt tgatgctcaa caaaatcaat catatgaagt
     7801 ttcaaaacca tttatttcaa ccgttagaac tgacgaaaac attattgata cccatgaagt
     7861 agtagaagaa tcattatcat tggtatcaaa agaaattgat ttcataaaac gagctcttgc
     7921 caaacacaaa ggcaaacgaa aattagcagc tcaggaatta gggatttctg aacgcacatt
     7981 atatagaaaa attaaagaat ataatattga agaataacat cattataagt atttgttgca
     8041 tactattggt tgcatcgtgt tcggttaaat attcatttag cggagcatca atagcgcccg
     8101 aaatcaaaac attttcggta gcatatatcg aaaatcaagc acctactaaa caagctactc
     8161 tcagcgatgt atttacagaa gggttaaaag aaaaaattag aacaaatact cgactttcaa
     8221 tggtaaataa ttatggcgat attctatttg aaggtgccat agtagattat tctgtaaatt
     8281 atttaggagt aacagcatca caactagcgg caagtaacag attaacaata actgtaaaaa
     8341 ttacatgcac aaacactaaa gaacctaaaa aaagtttcga aaaacaattt tcaagatatt
     8401 atgattttga tgccatgcaa aatatttcaa ctattgaaac agcagctatc aaacaaatat
     8461 cagatcaaat aattgaggat atatttttag aagcatttgc taattggtaa aaatacacta
     8521 tttttatagc atgaatagcg agctttttat acaatgtatt aaaaatcctt tagaaataga
     8581 ttcatctgca acagcagaac tcgaaaaact attggaagaa tttccgtatt ttc
//
LOCUS       DAOTOW010000007         8520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_7496_length_8520_cov_3.423981, whole genome shotgun sequence.
ACCESSION   DAOTOW010000007 DAOTOW010000000
VERSION     DAOTOW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7496_length_8520_cov_3.423981"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="P5243_00295"
     CDS             <1..1521
                     /locus_tag="P5243_00295"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HRS17914.1"
                     /translation="EGSQKGAKIIEKMQTYAALYDTTRAFTVASSGGWDTGIGTTVQI
                     MGYNYLKHGNIDEHHSKFPWQPSIGTEESNTVRTRGIYKTIDSICWLAPTNLYDEGME
                     GGWKFYDEREFLAGLFFWTGFDYHGEPTPYNWPAVISQFGILDLCGFPKDNAYYLHSW
                     WKQEPVIHIESNWNCIPGEQIDLVVYSNCATVELLINGRSLGKKSMQKNGHIVWPITF
                     EPGVLTARGYGSLVMPQTDSTKLLITKEFKTAGKPAAIQIIPHKKTLKPDGQDIAICT
                     IQIVDNKGTIVPDANIDITFEITGNASFIGVGNGNPSSHEKEVFQDNIIVKPIMIHKE
                     LPVQSLSKRPEIAVGFDDTKWRTAYSAEPSDWKEYSDTLLVMRGSFMLDAITKDMIIT
                     LFAKNILSNQVIYINGAMITPNAENAYVLPHAILKQGTNVYAVVGQKLKRPNQWEYPN
                     RDPGLIQIVTPVKQWQRKTFNGLAQIIVQSKTTPGKTTIKARAQGLPETELQIDIK"
     gene            1632..4439
                     /locus_tag="P5243_00300"
     CDS             1632..4439
                     /locus_tag="P5243_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRS17915.1"
                     /translation="MKKIFIVLSVTISLISCVSKNYKKNDTGVIIKIDSVNIQLQVYS
                     ENIIRVTAAVNKLPKDTSLVVKTPKKCFTDWSVTENKTHVIVNTKSLSASVNKTTGEV
                     VFTDAKGAIILQEKQGGGKTFIPKTFEGTNYYSVIQSFESPNDEAFYGLGGQHHGYMN
                     YKGKDVEIAQHNLVAAIPFIYSNKNYGILWDNSSISRFGDPREYSQISNLTLFDKQGK
                     QGGLTATYTIDGKVVKTTTDSIIDYRFLEEPGIESLSKEMKDKGTITWEGSLSSSIAG
                     LHKFLLYHSSYVKLWIDGELLFENWRQNWNPWSRPFTISLEPGNKKTIKLEWKIGGGF
                     LGLQHLNPLSNEEQNRFTLSAEAAYAIDYYFIYGTSADQVISGYRTLTGKATIVPKWA
                     MGFWQSRERYKTQQEIIDIVQTFRNKQIPIDNIVLDWHYWPTNQWGSHKFDTATFPNP
                     ELLCKQVHDLHCNIMISVWPKYYEGIDNYKAMKEQGFLYMNNIEKGRLDWVGPGYKNT
                     YYDAFNPKAREAFWNQIKPAIFDKGFDAWWLDATEPDMHSNISYHARKLNMHPTAVGT
                     GEQMFNAFAYVNAKGVYESQRQTAPDKRVFILTRSAYSGIQSVGAAVWSGDIVTRWSD
                     LKDQISIGINFSLSGAPFWTMDIGGFSIEDKFYKTTPDILEEWREIQTRWYQFGAFCP
                     LFRVHGQFPYREIFNIAPESHKAYKSMLYYNNLRYILMPYIYTLAAQTYHTDYTIMRG
                     LIMDFAHDSKVWNINDQYMFGPSLLINPVYQPGATKRSVYLPAGQGWYDLYSGIFFEG
                     GQTILANAPYDRMPIFVKEGSIIPTGQPMQYTNQMPDSIITLFVYGGKDASFTLYADE
                     ATNYNYEKGMFSMIPIHYNEKNKTITLEKRIGEFAGMLKSQTFKIVYIDKQQPKAFGN
                     LQTILEEMKYKGDKISIQLKK"
     gene            4473..5957
                     /locus_tag="P5243_00305"
     CDS             4473..5957
                     /locus_tag="P5243_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRS17916.1"
                     /translation="MLLLGFDIGSSSVKVSLIDGETGQNKASAFYPKQEMPIIAVKQG
                     WAEQNPEDWWTHLKAALQEVLSISAVQVSDIKAIGISYQMHGLVIVDKNHTVLRPSII
                     WCDSRATGIGAQAFNEIGREQCLSTLLNTPGNFTASKLKWIQQNEPEVYAKIHKIMLP
                     GDYIAMKLTGEITTTISGLSEGMFWNFATATPSETLLQYYGFDSSLLARIVPTFGIQG
                     YLNKQAAEELGLHTNITVSYRAGDQPNNALSLNVLEPGEIATTAGTSGIVYGVSEQIT
                     YDPLSRVNTFAHVNYTPNHIRLGVLLCINGTGILNSWIRKLVGNIPYETMNNMASKIA
                     IGSDGVSIIPFGNGAERVLQDTNVQAHIHNISFTTHSQAHIIRAAQEGIAFSFQYGME
                     IMKQIGIHPTTIKAGKANMFLSPIFRETIAGISGATIELYNTDGSIGAARGAGIGCGL
                     YSSTNEAFKGLTKLEEINPDVQNKTAYIDAYERWKKHVEQILNT"
     gene            6017..7330
                     /gene="xylA"
                     /locus_tag="P5243_00310"
     CDS             6017..7330
                     /gene="xylA"
                     /locus_tag="P5243_00310"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRS17917.1"
                     /translation="MGNKEYFKGINKIQFEGKESKNPLAFKWYNENQVVAGKTMKDHL
                     RFAVAYWHTLCGTGGDPFGPGTKNFPWEQASNVSDANKQRMDAAFEFITKLGAPYYCF
                     HDTDIAGDGSVFQIEKQLAEMVAYAKQKQAESGVKLLWGTANVFSNPRYMNGASTNPD
                     FAVVANAAVQVKNAIDATIELGGTGYVFWGGREGYMSLHNTNTKKELDHMGQFLRMAR
                     DYARKQGFTGTFFIEPKPMEPSKHQYDADVQTVLGFLNAQGLIDDFKLNVEVNHATLA
                     GHSFAHELRMAADAGKLGSIDANKGDYQNGWDTDEFPTNIYEITEAMLEILQAGGFNH
                     GGINFDAKTRRNSTDLEDIFIAHISGMDVFARSLIIADTILRESDYLKLRANRYASFN
                     TPKGIDFEAGKLSLTDMYAIAKEVGEPAMISGKQEYLEQLINQYI"
     gene            7400..>8520
                     /locus_tag="P5243_00315"
     CDS             7400..>8520
                     /locus_tag="P5243_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HRS17918.1"
                     /translation="MQTQVNMKFIAAITFVATLGGLLFGYDTAVISGAEKSIQAYLIT
                     SQGLSSLIHGATTSSALIGCIIGGAIAGIFSRKYGRKKTLMIAAALFFVSALGSGYPE
                     FLFFTKGEPTIGLLLMFNFYRIIGGIGVGLASAVCPMYIGEISPAHLRGRLVSLNQFT
                     IIFGMLVVYFVNYGIAKGQTIEWINDIGWRQMFLSEAIPAGLFGILLFFVPETPRYLA
                     LTNKDDSALAVLTKINGIDKAKEILAEIKNTVEHHSNNIFAFGKTVIFIGILLSVFQQ
                     FVGINVALYYAPRIFESMGAAKDASMLQTVVMGLINVIFTVVAILTVDRWGRKPLLMV
                     GSIGMAVGMFAIAALSYMHIIGISTLVFIMIYTASFMMS"
BASE COUNT         3027 a         1460 c         1561 g         2472 t
ORIGIN      
        1 gaaggaagtc aaaaaggagc aaaaattatt gaaaaaatgc agacatatgc agcattgtat
       61 gatacaactc gtgcatttac cgttgcatca agtggaggtt gggatacagg tattggaact
      121 accgttcaaa taatgggata taattattta aaacacggga atatagatga acatcatagt
      181 aaattcccat ggcaaccaag tataggaaca gaagaaagca ataccgtgag aacccgcggt
      241 atatataaaa ctattgattc aatttgttgg ctggctccta ccaatttata tgatgaaggc
      301 atggaaggag gttggaaatt ttatgacgaa cgtgaatttt tggcaggact atttttttgg
      361 acaggatttg actaccatgg agaaccaact ccatataact ggccagcggt aatttcgcaa
      421 tttggcatat tagatttatg cggttttcct aaagacaatg cgtattatct tcattcgtgg
      481 tggaaacaag aaccggtaat tcacattgag agcaattgga actgtattcc gggcgaacaa
      541 attgatttag ttgtatatag caattgtgct actgtagaat tacttataaa tgggcgaagt
      601 ttaggtaaaa aatcaatgca aaaaaatggt catatagtgt ggcctattac ttttgaacca
      661 ggtgttttaa cagcacgagg atatggttca ctggttatgc cgcaaacaga ttctacaaaa
      721 ttacttataa ccaaagaatt taaaactgcc ggcaaacctg ccgcaattca aataattcct
      781 cataaaaaaa cattaaaacc cgatggtcaa gatattgcaa tatgtactat tcaaattgtt
      841 gataacaaag gaactattgt acccgatgca aatatagata ttacatttga aattacagga
      901 aatgcctcat ttataggtgt tggcaacggc aatccatcaa gtcacgaaaa agaagtattt
      961 caggataaca taatagtaaa accaattatg attcataaag aattaccggt acaatcgctc
     1021 agcaaacgcc ccgaaattgc cgtaggtttt gatgatacaa aatggagaac agcatattcc
     1081 gcagaacctt cagattggaa agaatatagt gatacattat tagttatgag aggctctttc
     1141 atgcttgatg ctattacaaa agatatgata attacactgt ttgcaaaaaa tattttaagc
     1201 aaccaagtaa tatatattaa tggagctatg attactccaa atgcagaaaa cgcatatgtt
     1261 ttacctcacg ctatactcaa acaaggcact aatgtatatg ctgttgttgg acaaaaatta
     1321 aaacgtccca atcaatggga ataccctaat cgtgaccccg gattgattca aatagtaact
     1381 ccggtaaaac aatggcaacg aaaaacattt aacggattgg ctcaaataat tgtacaatct
     1441 aaaacaacac ccggaaaaac tactattaaa gctcgagctc aagggttgcc ggaaacagaa
     1501 ttacaaattg acattaaata attggatatg aaaagtaatt gtgttataca catcaataaa
     1561 aaaatatttt attcttgtaa aatacacttt acttcattat ctttgtgtaa aatttacaat
     1621 aactgaactg tatgaaaaaa atattcattg tactttctgt aacaatatca ttaatttctt
     1681 gcgtatcaaa aaattacaaa aaaaatgata caggtgttat tataaaaatt gattcggtta
     1741 atatacaatt acaagtatat tctgaaaata taatacgtgt tacagcagca gtaaacaaac
     1801 taccaaaaga tacaagttta gttgtaaaaa ctccaaaaaa gtgttttact gattggtctg
     1861 taactgaaaa taaaacacat gtaatagtaa acacaaaatc gctaagtgct tcggttaaca
     1921 aaactaccgg agaagttgta tttactgatg ctaaaggagc aattatattg caagaaaaac
     1981 aaggtggcgg aaaaacattt attcctaaaa cctttgaagg aacaaactat tacagtgtta
     2041 ttcaatcatt tgaatctccc aacgacgaag cattttatgg acttggcggg caacaccacg
     2101 ggtacatgaa ttataaagga aaagatgtag aaattgccca acataattta gttgcagcaa
     2161 ttccatttat atattctaat aaaaactatg gaatattatg ggataattcc tctatttcaa
     2221 gatttggaga cccacgtgaa tatagtcaaa tttcaaactt aacattattt gacaaacaag
     2281 gtaaacaagg aggactgaca gctacatata ccattgatgg caaagttgta aaaacaacaa
     2341 cagacagtat aatagattat agatttttag aagaacccgg tatagaaagt ttatcaaaag
     2401 aaatgaaaga taaaggaact ataacatggg aaggttctct atcatcaagc attgccgggt
     2461 tacacaaatt tttattatat cattcgagct atgttaaact ttggattgac ggcgaattac
     2521 tatttgaaaa ttggcgacaa aattggaatc cgtggtcgcg tccatttacc atatctttag
     2581 aaccaggtaa taaaaaaact ataaaacttg aatggaaaat tggtggaggc tttttaggat
     2641 tacaacacct caatccatta tcgaatgaag aacaaaacag atttacacta tctgccgaag
     2701 cagcttatgc tatagattat tattttattt atggaacctc tgcagaccaa gttatatcgg
     2761 gatatagaac actaaccgga aaagcaacta tagtacctaa atgggctatg gggttttggc
     2821 aaagccgtga acgatataaa acacaacaag aaataataga tattgttcaa acatttcgaa
     2881 acaaacaaat acctatagac aatattgttc ttgattggca ctattggcct accaaccaat
     2941 ggggttcgca caaatttgac acagctacat tccccaaccc tgaactactc tgtaaacaag
     3001 tacatgattt gcattgtaat atcatgattt cggtttggcc aaaatattac gaaggtatag
     3061 acaattataa agccatgaaa gaacaaggct ttttgtatat gaacaatatt gagaaaggac
     3121 gattagattg ggtggggccc ggttacaaaa atacctatta cgacgcattt aatccgaaag
     3181 ctcgcgaagc attttggaat caaataaaac ctgccatttt tgataaaggg ttcgatgcat
     3241 ggtggctcga tgctactgaa cccgatatgc attcaaacat atcataccat gctcgcaaat
     3301 taaatatgca tcctactgct gtaggtaccg gagaacaaat gttcaatgct tttgcttacg
     3361 ttaatgccaa aggagtttat gaatcacaac gacaaactgc tcctgataaa cgtgtcttta
     3421 ttcttacccg ttctgcatac agcggcattc agagcgtagg cgctgctgta tggagtggcg
     3481 atattgtaac tcgttggagt gatttaaaag atcaaatatc gataggaatt aatttttcac
     3541 tgtcgggtgc accattttgg actatggata ttggtggttt ttctattgaa gataaattct
     3601 acaaaactac acctgatatt ttagaagaat ggcgcgaaat tcaaacacgc tggtatcaat
     3661 tcggagcatt ttgtccacta tttagagtac atggacaatt tccatacaga gaaatattta
     3721 acatagctcc cgaatcacac aaagcgtata aatctatgtt gtattacaac aatctacgat
     3781 atatacttat gccatacata tacaccttag cagcacaaac atatcatacc gattatacta
     3841 ttatgcgtgg attaattatg gattttgccc atgattctaa agtttggaat ataaatgacc
     3901 aatatatgtt tggcccttcg cttcttataa atcctgtata tcaaccgggt gcaacaaaac
     3961 gctctgtata tttacccgca ggacaagggt ggtatgattt atatagcgga attttctttg
     4021 agggtggaca aactatttta gcaaatgctc cctacgatcg tatgccaatt tttgttaaag
     4081 aaggttctat tattcctacc ggacaaccta tgcaatatac caatcaaatg cctgactcta
     4141 taattacatt atttgtttac ggaggcaaag atgcaagttt tacgttgtat gccgatgaag
     4201 caactaatta taattatgaa aaaggaatgt ttagcatgat acctattcac tacaacgaaa
     4261 aaaataaaac aataacatta gaaaaacgaa taggagaatt tgcaggaatg ttgaaatctc
     4321 aaacatttaa gatagtatat attgacaaac aacaaccaaa agcatttgga aatctacaaa
     4381 ctattttgga ggaaatgaaa tataaaggtg acaaaatttc aatacaactt aaaaaataat
     4441 atagaaataa ttatttaact caaaattcaa aaatgttatt actcggattt gatataggaa
     4501 gttcatcagt aaaagtttct ctcatagacg gcgaaaccgg acaaaacaaa gcatcagcat
     4561 tttatccaaa acaagaaatg ccaattatag ctgtaaaaca aggatgggca gaacaaaacc
     4621 ccgaagattg gtggactcat ttaaaagctg cattacaaga agttttatct atatctgccg
     4681 tacaagtgtc tgacattaaa gcaattggta tttcatatca aatgcatggg cttgtaatag
     4741 ttgataaaaa ccatactgta ttacgcccct caataatatg gtgcgacagc agagctaccg
     4801 gtataggagc tcaagcattt aatgaaatag gcagagaaca atgtttatca acattactca
     4861 acactccggg taattttacc gcttctaaat taaaatggat acaacaaaat gaacctgagg
     4921 tatatgcaaa gattcataaa attatgttac ccggtgatta tatagctatg aaacttaccg
     4981 gtgaaattac aactaccata agtggacttt ctgaaggaat gttttggaat tttgccacag
     5041 ctacaccatc agaaacactt ttacaatatt acggctttga ctcatcatta cttgcccgga
     5101 tagttccaac gtttggcata caaggatatc tcaataaaca agcagccgaa gaattaggat
     5161 tacacacaaa cataactgta agttatcgtg ctggcgacca acccaataac gcactatcgc
     5221 ttaatgtgtt agaacccggt gaaatagcta ctacagcagg aacttcaggg atagtgtatg
     5281 gtgtaagcga acaaattacc tatgaccccc tttcacgagt aaacacgttt gctcatgtaa
     5341 attatacacc aaatcatatt cgattgggtg ttttactttg cattaacgga acaggaattt
     5401 taaactcatg gatacgaaaa ttagtaggaa atattcccta cgaaactatg aacaacatgg
     5461 cttctaaaat agcaatcgga agtgatggtg tatcaattat accttttggt aatggtgcgg
     5521 aacgagtttt acaagatacc aatgttcaag ctcatataca taatataagt tttacaactc
     5581 attcacaagc tcacataata cgagctgctc aagaaggaat agctttttca tttcaatacg
     5641 gaatggaaat aatgaaacaa ataggaatac atccaacaac aataaaagca ggaaaagcga
     5701 acatgttttt aagcccaatt tttcgtgaaa caatagcagg aatttcggga gcaactatag
     5761 agctatataa taccgatggt tcaataggtg ctgcacgtgg tgccggtatt gggtgcggtt
     5821 tgtattcatc aacaaatgaa gcttttaaag gacttactaa attagaagaa atcaaccctg
     5881 acgtacaaaa taaaacagca tatatagatg cttatgaacg ctggaaaaaa catgtagaac
     5941 aaattttaaa tacataaaca acttagttat atatacaata ctaataactc aaaatttata
     6001 aatcatggca gtagtaattg gaaacaaaga gtattttaaa ggaatcaata aaatacaatt
     6061 cgaaggaaaa gaatcaaaaa atcctttagc atttaaatgg tacaacgaaa atcaagtagt
     6121 tgcaggtaaa accatgaaag atcatcttcg ttttgcggtg gcatattggc acacactatg
     6181 cggcaccggt ggcgacccat ttggtcctgg aaccaaaaat tttccttggg aacaagcatc
     6241 aaatgtaagc gatgcaaaca aacaacgtat ggatgcggca tttgaattta ttacaaaatt
     6301 aggtgctccc tattattgtt ttcatgatac cgatattgcc ggtgatggtt ctgtttttca
     6361 aatagaaaaa caacttgccg aaatggttgc atatgctaaa caaaaacaag ccgaatcggg
     6421 tgtgaaatta ttgtggggaa cggcaaatgt attcagtaat cctcgctata tgaacggagc
     6481 ttctaccaat cctgactttg cagtagttgc aaatgcagcc gtgcaagtaa aaaatgcaat
     6541 agatgctacc atagaattgg gtggtaccgg ctatgtattt tggggtggta gagaaggtta
     6601 catgagcctg cacaatacca atacaaaaaa agaattagac cacatggggc aattcttacg
     6661 tatggctcgc gactacgcac gcaaacaagg atttaccgga acattcttta ttgaaccaaa
     6721 acctatggaa ccatcgaaac accaatatga tgccgatgta caaacagtat taggattttt
     6781 gaatgcacaa ggtttaatag atgatttcaa attaaatgta gaagttaacc acgctacttt
     6841 ggcagggcat tcatttgctc acgaacttcg tatggctgct gatgccggca aattaggaag
     6901 catagatgca aataaaggcg attaccaaaa cggctgggac actgacgaat tcccaacaaa
     6961 tatttatgaa attactgaag ctatgctcga aattttacaa gcaggcggat tcaatcatgg
     7021 cggtataaac ttcgatgcaa aaactcgcag aaactctacc gatttagaag atatatttat
     7081 tgctcacatt agtggaatgg acgtatttgc tcgctcatta atcattgccg atactatttt
     7141 acgcgaatct gattatttga aattgcgtgc taacagatat gcatcgttca atacaccaaa
     7201 aggaattgat tttgaagcag gcaaactttc gcttaccgac atgtatgcta tagcaaaaga
     7261 agttggcgaa cctgcaatga ttagtggcaa acaagaatat ttggaacaat taattaatca
     7321 gtatatataa tgtattagga gttagagagt cacactaact ctctaactca tttttatata
     7381 ccattaaaac cccaaaccta tgcaaacaca agtaaacatg aaatttatag cagcaattac
     7441 atttgttgct acacttggcg gactcctctt tggatatgat actgccgtaa tttccggtgc
     7501 cgaaaaatct attcaggcct accttattac cagccaaggg ttaagttctc ttattcacgg
     7561 agccacaaca tcgagtgctc ttattggttg tatcataggc ggggcaattg caggtatttt
     7621 ttctcgaaaa tacggtagaa aaaaaacgtt gatgattgcc gctgcattat tttttgtttc
     7681 ggcattaggt tcaggctatc ccgaattttt atttttcacc aaaggtgaac ctaccattgg
     7741 attgttactt atgtttaatt tttatcgaat tataggtggt attggtgtag gcttggcatc
     7801 ggcagtatgc ccaatgtata ttggagaaat ttctcctgct catttacgcg gacggttagt
     7861 ttcgctcaac cagtttacca taatttttgg tatgttggta gtatattttg taaattacgg
     7921 aatagccaaa ggacaaacca tagaatggat aaatgatata ggttggcgac agatgtttct
     7981 ttcggaagct atacctgcag gattatttgg tatattatta ttttttgttc ccgaaactcc
     8041 tcgttatttg gcattgacca acaaagatga ctcggcattg gctgtactaa ccaaaatcaa
     8101 cggtattgat aaagccaaag aaattcttgc cgaaatcaaa aataccgttg aacaccattc
     8161 aaataacatt tttgcatttg gtaaaaccgt tatttttatt ggtattttat tgtctgtttt
     8221 ccaacaattt gtaggtatta acgtggcatt gtattacgca ccgcgaattt ttgaaagtat
     8281 gggagctgcc aaagatgctt cgatgctgca aacagtagta atgggactta taaatgtaat
     8341 ttttaccgta gttgctatac ttactgtaga ccgttgggga cgtaaaccat tgctcatggt
     8401 aggctctata ggtatggcgg ttggcatgtt tgccattgcc gctttatcat atatgcatat
     8461 aattggaata agcacattag tgtttattat gatatacact gcatcgttta tgatgagttg
//
LOCUS       DAOTOW010000008         8111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_7937_length_8111_cov_3.303749, whole genome shotgun sequence.
ACCESSION   DAOTOW010000008 DAOTOW010000000
VERSION     DAOTOW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7937_length_8111_cov_3.303749"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="P5243_00320"
     CDS             <1..940
                     /locus_tag="P5243_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17919.1"
                     /translation="FLLLFSCILVQCTQKEKIIYGALAFIDGVEILDIEPFQDSLLLI
                     AGKNKQNQGFLYVYNTKTKKYFASQTTHVMYDIFLEDTVLWACGDSMSIIKSNDKGLT
                     WNVYNNFNYFWDADKSHFRKLAIYNNTPIYAIGKKDMLQGNMYITNNSVTYPFKSIQM
                     QTGINDMAIIDSSQVYVAAYGSIIRFTNNGKNKRFEQIGGHNFTSITYSGNKNIISTT
                     FDGSIFTCELTDSVWKKVLNTSIEFKHIASDSYGNAIAIGNSNTIYISNDYGNHWHLE
                     KYAFGKDVTCLTYANNLFYIGCKSGEIHTITRSQIE"
     gene            943..1992
                     /gene="mltG"
                     /locus_tag="P5243_00325"
     CDS             943..1992
                     /gene="mltG"
                     /locus_tag="P5243_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRS17920.1"
                     /translation="MTKKNTKTKGLIIIGIFLGVLLVSGSWIIYSFFFKPSVHFTNGS
                     DSYLFAIPTGSNISNVIEKLQKETQWNSTTGFMILSKVLDLENHIYPGLYKLSANMNN
                     KDLALLFRSGKRETVDVVIRFGRYAQDIARTIAPKLEADYDELTALLQNSSFLDSLGF
                     TKETAICLFLPDTYEFKWNTNAIEFIQRMHKEYNTFWNNERLQKAQSIGFTPQQIMTL
                     ASIINQETNKNDEKPKIAGVYINRLKQNMPLQADPTVKYAVGDFSLKRIYHGHLQVNS
                     PYNTYRNIGLPPGPICTPQKSDIEAVLNYENHSYLFFCARPDYSGYHNFAKTYNEHLQ
                     FAGAYSKWLNQEQIR"
     gene            complement(1996..2361)
                     /locus_tag="P5243_00330"
     CDS             complement(1996..2361)
                     /locus_tag="P5243_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS17921.1"
                     /translation="MNILVAEDEILNSMLIERVLQKMGHYCTVVANGQHVIQQCNSQL
                     FDLLIVDVSMPVLDGLKAIETIRNTQNANKHTPILLLSATYPHNYNQIKELYQIQDIV
                     LKPFTVQDIAKKIEIIQQK"
     gene            complement(2358..3005)
                     /locus_tag="P5243_00335"
     CDS             complement(2358..3005)
                     /locus_tag="P5243_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS17922.1"
                     /translation="MYNLELLESIVQQAFVNAERGHDYWHAMRVVQNARYISSIEGGN
                     ISIIEASAWVHDMVDEKFTSLEHSIDSVNTILLQCGFSKHECETIIYIISNISFKGGL
                     STVENPSIELQIVQDADRIDAMGAIGIARAFHYGGFKNRELYNPTIEPEIFKTESEYR
                     NSQGHTINHFYEKLFTLYSQMNTSAAKRIAKQRHEYMETFVSQFLHEWNGNFDVI"
     gene            complement(3005..3298)
                     /locus_tag="P5243_00340"
     CDS             complement(3005..3298)
                     /locus_tag="P5243_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17923.1"
                     /translation="MKAVMITYNQAHTERVEYILDRLEIKGFTRWADVQGRGSETGEP
                     RMNTHTWPENNSATLTMVDDSKVADLLASIKKLDEINKEVGIRAFVWNIEQMY"
     gene            complement(3376..6501)
                     /locus_tag="P5243_00345"
     CDS             complement(3376..6501)
                     /locus_tag="P5243_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS17924.1"
                     /translation="MSIYSSSVKNPITTIMIFLAVIVFGLYSLSSLPIDLYPKMEPPY
                     ISIMTTYPGASAADIETNVTKPIEDALNSVDNLKNISSRSIDNMSVVFAEFEWESNLD
                     EAANDIRNSLSFVEEALPDDCKKPTIFKFNSSMMPILFYAVTAKESYPALNKILEEKL
                     INPLNRIEGIGSIGLEGTPKREIAIEVNPQKLEAYNLTVEDIGNVLKAENINMPLGKL
                     EMGAMDYSLRVEGEFKESSVIQNIVIGSYMGKSIYLKDVAYVRDTIKKRTIDQKINGE
                     NGIRLFVMKQSGANTVKITKQVNAEIEKLKKTLPPDIQLIQIFDSSVFINHSISNLTE
                     TLMWAFLFVVLVVIFFLGRWRATFIIVLTIPISLIVAFIYLKLSGNSINIISLSALSI
                     AIGMVVDDAIVVLENISKHIDRGASPREAAIYATNEVWLAVIVTTLTVVAVFLPLTMV
                     TGMTGVLFKQLGWIVTITVVTSTIAAISLTPMLSSKMLRLKPKKLTERKWSYDNIVIR
                     FLDTLDAFYEKSIRWALAHKKTVVFSSIGIFVASIFLVSQVGSEFIPETDQSTLNITV
                     ELQTGTRVEKTVEVTRKITEIIETKYPEVKVRFSSSGSDSEGDLTSLFSESGSHMINF
                     RRMRLTQPSERNRTVWEIADALRKDLDGIAEIEKYSVSTQDGMSFGGGNAISVEIYGY
                     DFAKTDALARQIADSIKSLPGARDVKISRKKEKPELKVVLDQEKMALNGLNNAMVSMA
                     LRNRIQGYTSTKYREKGDEYDVVVRFAEEDRNTITEIENISIKNMYGKMIRLKEIGKV
                     EEHWAAPNIEHKRRERIVTVSASPHNVSLGDLSKRIQKQLDSIEKPSEILVNMGGSFE
                     DMQESFADLGLLMILVLLLVYIVMAAQFESLKMPLIIMFSIPFAFSGVFIALFISGKT
                     FSVIAALGAIMLIGIVVKNAIVLIDYINLMRERGYELNEAISLSGRSRLRPVLMTAAT
                     TVLGMVPMALSKGEGSEIWSPMGIAVIGGLIFSTIITLIIVPVGYNLMARKGEMNKKK
                     QLEKQFHFLDN"
     gene            complement(6520..7572)
                     /locus_tag="P5243_00350"
     CDS             complement(6520..7572)
                     /locus_tag="P5243_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS17925.1"
                     /translation="MKTVVNITAILTCVCIMASCGGQKKEDKTVKTDSTEQVKAKPVK
                     VMPITLQEVAIEEVYPATIQAWQEAHVGPASPGQIHKIYVNVGDKVSKGTLLAQMDPT
                     QYNQLRIQYQDAKRDLQRMDSLIKYGNISQQTYDKVKLQNEVLQTNLQTLSDNVFLKA
                     PFSGVITGKYFNERENYSGMAAAVGVGAIFTIMQVNELKVVVSVSERFFPIVKKGMKT
                     KLLTDIYPDKKFEGVVETIYPTIDASTKTFKIEIKVPNPQEILRPGMFARIAFDFGKA
                     KAMVVPANAILKQEGTNERYVFIYNNGKAQRITVELGTRFNDQIEIRSDKITPGMQLI
                     YTGYTGLIDEQVVEIQ"
     gene            complement(7598..>8111)
                     /locus_tag="P5243_00355"
     CDS             complement(7598..>8111)
                     /locus_tag="P5243_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS17926.1"
                     /translation="DYKLLESQEMLAQKSVLLEKMSFAPTAVAFYNYNYKIITTDFDM
                     NPDHIAGVSLKIPLTSSGSRSLKVQEAKIKYEQAQNTKALVAQQLQIKEKQARYDVAS
                     AYENYMLQKENIALSLQIYEKVRQKYEYGVASSFELTQANNEYLQAQSNYLNAYMQLL
                     QAKTALEKLL"
BASE COUNT         2668 a         1432 c         1153 g         2858 t
ORIGIN      
        1 tttcttactg ctgtttagtt gtatattagt acaatgtaca caaaaagaaa aaattattta
       61 cggagcatta gcatttattg atggtgtaga aatacttgac atagaacctt ttcaagattc
      121 attattactt atagcaggta aaaacaaaca aaatcaaggt tttttatatg tatataatac
      181 taaaacaaaa aaatattttg cctcacaaac aactcatgta atgtacgata tttttttaga
      241 agacacggtt ctatgggcat gcggcgacag tatgagtatc attaaaagta atgacaaagg
      301 gttaacatgg aatgtgtata ataatttcaa ctacttttgg gacgcagata aatcacattt
      361 cagaaaactt gcaatttaca ataatactcc tatatatgcc attggcaaaa aagatatgct
      421 tcaaggaaat atgtatatta caaacaatag tgtaacatat ccgtttaaaa gtatccaaat
      481 gcaaacagga atcaatgata tggcaattat tgacagttcg caggtatatg ttgcagcata
      541 tggttcaatt attcgcttca caaataatgg taaaaataaa cgatttgaac agataggtgg
      601 ccataatttt acaagtatca catattcagg taacaaaaat attataagta ccacgtttga
      661 tggcagtata tttacctgtg aattaactga ttctgtatgg aaaaaagttt tgaatacatc
      721 aattgaattt aaacacatag cctctgattc ttatggaaat gctatagcta taggaaatag
      781 taatactata tacattagca atgactatgg caatcattgg catttagaaa agtatgcttt
      841 tggtaaagat gttacttgtc tcacctatgc aaacaattta ttttatatag gctgcaaatc
      901 aggtgaaatt cataccatta cacgttcaca aatagaataa caatgacaaa aaaaaataca
      961 aaaactaaag gactaataat tattggaatc tttttaggtg ttttgcttgt atcaggttct
     1021 tggattattt attcattttt tttcaaacca tcggtacatt ttactaacgg ttctgattca
     1081 tatttatttg caattcctac cggttctaat atttcgaatg taatagaaaa attacaaaaa
     1141 gaaacacaat ggaatagtac tacaggtttt atgattcttt caaaagtttt agatttagaa
     1201 aatcatatat atccaggttt atataaactt tcggctaata tgaacaataa agatttggca
     1261 ttattgtttc gttcgggcaa acgagaaact gttgatgtag taatacgttt tggacgatat
     1321 gcacaagaca ttgcacgtac aattgctccc aaattagaag ccgattatga tgaattaact
     1381 gctttattac aaaatagcag ttttttagat tctttagggt ttactaaaga aactgccata
     1441 tgtttatttt tacctgatac ttatgaattt aaatggaata caaatgccat agaatttata
     1501 caacgaatgc ataaagaata taacacgttt tggaataatg aacgtttaca aaaagctcaa
     1561 tcaataggat ttacgcctca acaaattatg actttagcct ctataataaa tcaagaaaca
     1621 aacaaaaatg acgaaaaacc taaaattgca ggtgtataca taaatcggtt aaaacaaaat
     1681 atgccactcc aagcagaccc tactgtaaaa tatgctgtag gtgattttag tttaaaacga
     1741 atttatcatg gtcatttaca agtaaattct ccatataata cttatcgaaa tatcgggttg
     1801 cctcccggtc caatttgtac cccacaaaaa tcagatatag aagctgtatt gaattacgaa
     1861 aaccattctt atttattttt ctgtgcacgc cccgattatt ccggatatca taattttgca
     1921 aaaacataca atgaacattt acaatttgcc ggagcataca gcaaatggct aaatcaagaa
     1981 caaattcgat aatacttatt tttgttgtat tatctctatt ttttttgcaa tgtcttgtac
     2041 agtaaaaggt tttaaaacta tatcttgtat ttgatacaat tctttaattt gattataatt
     2101 gtgaggatat gttgccgaaa gcaataaaat tggagtatgt ttatttgcat tttgtgtatt
     2161 tctaatagtt tctatagcct ttaaaccatc aagtacaggc attgaaacat ctactataag
     2221 taaatcaaat aactgcgaat tacattgttg tattacatgt tgaccattag caacaacagt
     2281 acagtaatgc cccatttttt gtaggactct ctctattagc atactattaa gtatttcatc
     2341 ttcagctacc aatatattca tatcacatca aaattaccgt tccattcatg taaaaattgt
     2401 gatacaaaag tttccatata ctcatgtcgt tgctttgcaa tacgctttgc tgcagaagta
     2461 ttcatttgag aatataatgt aaataatttt tcataaaaat gattaattgt atgcccttga
     2521 gaatttctat attccgattc ggttttaaat atttctggtt caatggttgg attatacaat
     2581 tctctatttt taaaacctcc ataatgaaat gctcgtgcaa ttcctatagc tcccatagca
     2641 tcaattcggt cggcatcttg tactatttgc aattctatac taggattttc aacagtagat
     2701 aaaccgcctt taaatgaaat atttgaaatt atataaataa tagtttcaca ttcgtgtttg
     2761 ctaaacccac attgtaaaag tattgtattt acagaatcaa tagaatgttc caaagaagta
     2821 aacttttcat ccaccatatc atgtacccac gcagaggctt ctatgattga aatattacct
     2881 ccttcaatag atgaaatata acgagcgttt tgaacaacac gcattgcgtg ccaataatcg
     2941 tgtccacgtt cggcatttac aaacgcttgt tgtacaatag attctaataa ttctaaatta
     3001 tacattaata catttgttct atattccaca caaaagcacg aatacctact tctttattta
     3061 tttcatctaa tttttttata ctggctaata aatcagctac tttactatcg tcaaccatag
     3121 ttaaagttgc agaattattt tcaggccacg tatgagtatt cattctcggc tcacctgttt
     3181 cagaacctct tccctgcaca tcagcccatc gtgtaaaacc ttttatttct aatctatcaa
     3241 gtatatactc aactcgttcg gtgtgagctt gattatatgt tatcattact gctttcataa
     3301 ttttttaaat aattttaagg taacctaaga cacaatagga ataaaccatt gtgtcttagt
     3361 atgaatattt taatattaat tatcaaggaa atgaaattgt ttttccaatt gttttttctt
     3421 attcatttca cctttacgag ccattaaatt gtagccaacc ggcacaatta taagagtaat
     3481 aatagtagaa aatattaatc caccaattac tgcaataccc ataggactcc aaatttcaga
     3541 accttcacct ttactcaatg ccataggaac cataccaagc acagttgtgg cggctgtcat
     3601 aagtaccgga cgtaaacgac tgcggcctga caatgaaatt gcttcattta attcatagcc
     3661 ccgttctctc atcaaattaa tataatctat aagaacaatt gcatttttta caacaatacc
     3721 tataagcatt atagcaccta aagctgctat tacactaaat gttttaccgc taataaataa
     3781 tgctataaat acacctgaaa atgcaaatgg tatagaaaac atgataatta acggcatttt
     3841 aagcgattca aattgagcag ccattactat atacactaac agtaacacca aaatcataag
     3901 taaccctaaa tcggcaaatg attcttgcat atcttcaaaa ctaccaccca tattcactaa
     3961 aatttctgat ggtttttcaa ttgaatccaa ttgcttttgt attcgcttag ataaatcacc
     4021 taaagacaca ttatgtggtg aagcactaac ggttactata cgttcacgtc ttttatgttc
     4081 aatatttggt gcagcccaat gttcttctac tttgccaatt tctttcaatc gtatcatttt
     4141 tccatacata tttttgatag aaatattttc aatttcagtt atagtatttc tatcttcttc
     4201 ggcaaatcga actacaacat catattcatc acctttttct cgatatttag tgctggtgta
     4261 tccttgaatt ctgttacgca gagccataga aaccatagca ttatttaacc cattcaaagc
     4321 cattttttct tggtcgagta caacttttaa ttccggtttt tcttttttgc gacttatctt
     4381 aacatcacgt gcaccgggta aacttttaat actatcggca atttgacgag ccagagcatc
     4441 tgttttcgca aaatcatacc cgtaaatttc aaccgaaata gcattaccgc caccaaagct
     4501 cataccgtct tgcgtagaaa ccgaatactt ttcaatttct gcaattccat ctaaatcttt
     4561 tcgtaatgca tctgctattt cccatactgt tctatttcgt tcacttggtt gtgtcaaacg
     4621 cattcttcta aagttaatca tgtgcgagcc cgattcacta aataaactgg ttaaatcacc
     4681 ttcagaatca cttcccgaag aactaaaacg aacttttact tcaggatatt tagtttcaat
     4741 aatttcagta atttttcgcg taacttctac tgttttttct actcgtgtac ctgtttgtaa
     4801 ttctactgta atatttaatg tactttgatc ggtttcggga ataaattctg aaccaacttg
     4861 agaaactaaa aagattgaag ctacaaatat acctattgaa ctaaacacta ctgttttttt
     4921 atgagccaat gcccaacgaa tcgatttttc ataaaacgca tcgagtgtat caagaaaccg
     4981 tatcacaata ttgtcataag accatttgcg ttcagttagt ttttttggtt ttaaacgcaa
     5041 cattttagaa cttaacattg gggtaagtga aatagctgct atagtagaag ttactacagt
     5101 aattgttaca atccaaccaa gttgtttaaa taatacaccg gtcatacctg ttaccatagt
     5161 taatggtaag aatacagcta ccactgtaag tgtagttaca attactgcca gccatacctc
     5221 attggtagca taaattgcag cttcacgagg actcgcacct ctatctatgt gtttagaaat
     5281 attttcgagt acaactatgg catcgtctac caccatacct attgctatag atagagccga
     5341 aagcgaaatg atatttatag aattacccga tagtttaaga tatataaatg caacaatcaa
     5401 tgatattgga atagtgagaa ctattataaa cgttgctctc caacgtccca aaaagaaaat
     5461 taccactaac acaacaaaca aaaacgccca cattaatgtt tctgtaaggt tactaataga
     5521 atgattaata aatacagaac tatcaaatat ttgtattaac tgaatgtcag gaggtaatgt
     5581 tttttttagt ttttcaatct cagcatttac ttgtttggta attttaactg tattagctcc
     5641 tgattgtttc atcacaaaca atcgaatacc attttctcca ttaatttttt gatctattgt
     5701 tcgtttttta atagtatcgc gaacataagc aacatctttt agataaatgc ttttgcccat
     5761 ataactacca attacaatgt tttgaattac actactttct ttaaattcgc cttcaacacg
     5821 cagagaatag tccatggcac ccatttctaa ctttccgaga ggcatattta tattttcggc
     5881 tttcaaaaca ttaccaatat cttctacagt aagattgtat gcttcaagct tttgaggatt
     5941 cacttctatg gctatttctc gcttaggagt accttctaaa ccaatagaac caatgccttc
     6001 aatacgatta agaggattta taagcttttc ttctaaaatt ttatttaatg ccggataact
     6061 ttcttttgca gttacagcat aaaaaagtat aggcatcatg cttgagttaa acttaaaaat
     6121 tgtaggtttt ttacaatcat cgggtaaagc ttcttcaaca aaactcaaag aatttctaat
     6181 atcatttgca gcttcatcta aattactttc ccattcaaat tcggcaaaca ctacagacat
     6241 attatcaata gaacgagaac taatattttt gagattatca accgagttta acgcgtcttc
     6301 tattggtttg gtaacatttg tttcaatatc ggcagcactt gcacccggat aggttgtcat
     6361 gatagaaata tacggaggct ccattttcgg atacaaatct attggcaaac ttgacaaaga
     6421 gtataagcca aatactataa cagccaaaaa tatcattatg gttgttatag gattttttac
     6481 tgaactacta tatatgctca tattagtata tttttaaatt tattgtattt caacaacttg
     6541 ttcatcaatt aaaccggtat atcctgtata aataagctgc attccgggtg taattttatc
     6601 gcttctaatt tctatttgat cgttaaatcg agtacctaat tcaacggtga tacgctgtgc
     6661 tttaccatta ttataaataa atacatatcg ttcatttgta ccttcttgtt ttagaatagc
     6721 gttagcagga actaccatag ctttagcttt tccaaaatca aatgctatac gagcaaacat
     6781 tcccggacga agaatttctt gcggatttgg aactttaatc tcaattttga acgtttttgt
     6841 tgatgcatct atagttggat atattgtttc tacgactcct tcgaattttt tatcgggata
     6901 tatatcggtt agtaattttg ttttcattcc tttttttaca attgggaaaa agcgttcact
     6961 aacacttacc acaactttga gttcattaac ctgcattatt gtaaaaattg caccaacacc
     7021 aactgcagca gccattcccg aataattttc gcgttcgtta aaatatttac cggtaattac
     7081 gcctgaaaat ggtgctttaa gaaacacatt atctgataat gtttgcaaat tagtttgcaa
     7141 tacttcattt tgcagtttta ctttatcata ggtttgctgc gaaatattgc catatttaat
     7201 aagagaatcc atacgctgta aatcacgctt agcatcttga tactgaatac gtaattgatt
     7261 atattgtgta ggatccattt gagccaacag ggtgccttta gaaactttgt cgcctacgtt
     7321 tacatatatt ttatgtattt gtccaggtga tgcagggcct acatgagctt cttgccaagc
     7381 ttgaattgtt gccggatata cctcttctat agcaacttct tgcaaagtta taggcataac
     7441 ttttaccggt tttgctttaa cttgctctgt actatctgtt tttactgttt tatcttcctt
     7501 tttttgtccg ccgcacgatg ccattatgca tacacacgta agaattgctg tgatgtttac
     7561 tactgttttc atatacgttt atttttgtta atatcattta caataatttt tctaatgctg
     7621 tttttgcttg taacaattgc atatatgcat tcaaataatt ggattgagcc tgcaaatatt
     7681 cattgtttgc ttgagtaagt tcaaaacttg atgctactcc atattcatac ttctgtcgta
     7741 ccttttcata tatttgaagc gacaaagcaa tattttcttt ttgtaacata taattttcat
     7801 atgcacttgc aacatcataa cgagcttgtt tttctttaat ttgtaattgt tgagcaacta
     7861 atgcttttgt attttgagct tgttcgtatt taattttagc ttcttgaact tttaaacttc
     7921 tacttccgct tgatgttaaa ggtattttta atgaaacacc tgcaatatgg tcaggattca
     7981 tatcaaaatc ggtagtgata attttataat tataattgta aaacgctaca gcagtaggag
     8041 caaaactcat tttttcgagt aataccgatt tttgtgctaa catttcttgc gattccaaca
     8101 atttataatc a
//
LOCUS       DAOTOW010000009         7759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_8329_length_7759_cov_3.653686, whole genome shotgun sequence.
ACCESSION   DAOTOW010000009 DAOTOW010000000
VERSION     DAOTOW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8329_length_7759_cov_3.653686"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="P5243_00360"
     CDS             <1..534
                     /locus_tag="P5243_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRS17927.1"
                     /translation="GKTYTAVALAVRALKNREVQRIILCRPAVEADEKLGFLPGDMRE
                     KLNPYLQPLYDALLDMMPPKRLQDLIQDNVIQIAPLAYMRGRTLDNAFVILDEAQNAT
                     LNQLKMFLTRMGERAKFIITGDITQIDLPDKNRSGLQKVLDMFKQSKGIETIYFENKD
                     VVRHKLVKHIIEAFEKV"
     gene            complement(793..3354)
                     /locus_tag="P5243_00365"
     CDS             complement(793..3354)
                     /locus_tag="P5243_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS17928.1"
                     /translation="MKYFIISIFFIFSVFFANAQSPQLRQKLQDAEYFFLNEDYEQAL
                     KMYKDILKMDPKNFNVNYKIGLCYQRMPFEAGRSIPYLQKAVTGISKAYVEGSITEKK
                     APLQAMYHLAYAYHTNKYYDEALAMYQRYIDTLPISDNYHFQIAQRQLQSCRNAKEIS
                     KHPVPVEIFNVGNTINSAWDEFNPCPTSDGKLLFYTRLVEIKQTTKKGEDDANKKVYK
                     IMYTESLGDDKWTEPKDISDQLKTGGLCNTVSVNADGTFLILYKNTWENGGMTDISTG
                     TLYYSTRKSRKDPWTPMKKFNKNISSVNNETHAMISPDGNRLYVTSDRKGGFGGLDIY
                     VSEKTKGDWGPLRNLGPTINTAFDEQTPIICEDGVTLYFSSEGHYNMGELDIFKTTMI
                     AKNVWSEPVNIGAPINSPENNAYFYPIMNGKQAFYALARHEGYFTFGASDIYQCEFLD
                     VKPQEKELPVTLQGVVSFDDNKPLDYTVAVTVVNVATKDTVKKITPNEAGEYSLVVPA
                     GEYKIYFTRPKYNSVEKHVSIAKTTVEKTVQVNAVMYPEAVDNKKYYVIRNIYFDLNS
                     YELNRDALIEANKLLTIMMENPSLYIEVIGHTDSYGNADYNQKLSENRSRAVIDYLAK
                     NGVEYERFVSTAAGTTRKAVMDVTESGVVDADKAKLNRRVEIRVLKTKGDAEIIVDNR
                     PDELKFKEFNRFSILLLDTVAKQDTKKFTNLGDNVTVMEFANKNGTYYYYYGDFKSKA
                     EASVALNNAIDKGYTKAQIIDYFALNTSNDFVINNPVKFTKKYTIQLQALEKPLILHA
                     NPIMAEATRYQTKDGYYRYTYKEYDDMKEAELDLKKVIEYGFTDAVIVEVSKLKN"
     gene            3468..4373
                     /locus_tag="P5243_00370"
     CDS             3468..4373
                     /locus_tag="P5243_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRS17929.1"
                     /translation="MNGSKNNNKIPVYSLQSFIQSNVFNSYFQVEPFDTNRHFQVAYP
                     HRHDFYEVLFLQKGSGFHIIDSNTYTINPPCVFFLSPGQAHKLELSQDIDGFIFLFTT
                     EFYALFDFNKNKLLEFPFFFSVEQSNPPLYITNSSDFTFLKELFARAYNESAGNITNM
                     EVIHTILQLILLTCNQLYTKNTATYTPKKSQIIVKKFLLALEETYIYNHSIEYYAKQL
                     AITPNHLSQIVKQITGKSTSSIIQEKRLIEIKRLFLHTDMSIGHIAEHLHFNDISYMT
                     KFFKKYMKITPLFYRENELKKANMK"
     gene            4536..6011
                     /gene="ilvC"
                     /locus_tag="P5243_00375"
     CDS             4536..6011
                     /gene="ilvC"
                     /locus_tag="P5243_00375"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRS17930.1"
                     /translation="MANYFNSLPFRLQKIELGKCDFMDSSEFDKGVEYIKGKKIVIVG
                     CGAQGLAQGLNLRDSGLDVSYALRKIEIDENQISYQNAKNNNFVVDTIENLVPQGDLI
                     LNLTPDKYHTPVVNHIMPLMKKGACLSYSHGFNIVEEGMQIRKDITVIMVAPKSPASE
                     VRAEFLRGFGVPTLIAVHRENDPNGDGLEIAKAYCVGTGGHKAGVLHSSFVAEVKSDL
                     MGEQTILCGLLQTGSILCFNKMVEKGIDAGWASKFVQYGWEVITEALKIGGITHMMDR
                     LSNPAKIKAYYLAEELKQIMRPLFEKHQDDILSGHFSKTMMEDWANGDKNLLTWRAAT
                     AETAFEKTPAADVEISEQEYFDKGVLMVAFVKSGVELAFELMTESGMKPESAYYESLH
                     ETPLIANTIARKKLYEMNRVISDTAEYGCYLFDHACKPLLADFMSKIDTDVIGKEYNA
                     GKNNKVDNMELVKINEAIRYHDIEICGEELRAAMTAMKPII"
     gene            6226..7353
                     /gene="mqnE"
                     /locus_tag="P5243_00380"
     CDS             6226..7353
                     /gene="mqnE"
                     /locus_tag="P5243_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HRS17931.1"
                     /translation="MTHDDVLLALNETAVSQELQTIAHKVLQNQRITTQDCLLLYEQG
                     ELAFVGALADFVRFQKNENIVFFNKNIHIEPTNICIHNCTFCSYSKKKGDPLCWEMSI
                     DEMLAIITKIPEHAITEVHIVGGVHPDRGIDFYCDLLTAIKNIRPSIQIKAFTAVEID
                     FMAKKSGFFVSEILQKLKDAGLQTMPGGGAEIFDESLRKIICPTKTSSERWLKIHETA
                     HKTGIPTNATMLYGHIEKFEHRVDHLNRLRNLQDKTNGFQAFIPLKYKKENNYLGIEH
                     EVPWSEDLRNFAISRIFLDNFKNIKAYWPMLGKELSQMTLEFGVNDFDGTINDSTKIY
                     SMAGAEDTHPTLTEEQMVALIHAVGRKAIERDSVYNIIQTH"
     gene            7359..>7759
                     /locus_tag="P5243_00385"
     CDS             7359..>7759
                     /locus_tag="P5243_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17932.1"
                     /translation="MNSTWIHTLWKPLYNKIILIIVCGIIPLIIYNKWDIVLIINKYC
                     TNDLLDVFFMYYTNTALGGIFVICTCICVFYSYRKASMIALSGIIILITSVVLKQIIF
                     QGYPRPTAEIPHNQFFHIIDGFTYARHNSFPS"
BASE COUNT         2700 a         1290 c         1154 g         2615 t
ORIGIN      
        1 ggaaaaacat ataccgctgt tgctttggct gtgcgagctc ttaaaaacag agaagttcag
       61 cgtattattc tatgtagacc tgctgttgaa gccgatgaaa aattaggatt tttacccggc
      121 gatatgcgcg aaaaattaaa tccatatctg caacctctct atgatgcact gctcgatatg
      181 atgcctccaa aacgattaca agatttaatt caagacaacg taatacaaat agcaccactt
      241 gcatatatgc gtggtcgcac tttggacaat gcatttgtaa ttctcgatga agctcagaac
      301 gcaacactca atcaactcaa aatgtttttg actcgtatgg gagaacgagc aaaatttata
      361 attacaggcg atattactca gattgattta cccgataaaa atcgttcggg acttcagaaa
      421 gtacttgaca tgtttaaaca aagtaaagga attgaaacta tatatttcga aaataaagac
      481 gtagtaagac acaaattagt taaacatata attgaagcct ttgaaaaagt gtgatataat
      541 ttttaattct taattataaa tttttaatta aaaatgctgt gttataaact ctaagtgaca
      601 ccattacaca caacactcca cgaaaaaata tattcagacg aaaatgttat tctatttcgt
      661 tggttgtatg gttctatgaa actctgtgtt tttcgttgaa gctagattgt atgaaataaa
      721 gcgaccacta tattgaaaaa aaagggcaaa cacattggtt tgccctatat attgaataat
      781 ataagtatcc ttttaatttt tcaatttact aacctcaaca attacagcat cagtaaaccc
      841 atattcaatt acttttttaa ggtcaagctc agcttctttc atatcatcgt attctttata
      901 agtatatcta taatatccat cttttgtttg atatcgagtt gcttcagcca taattggatt
      961 tgcatgaaga atcaatggtt tttctaacgc ttgcaattga atagtatatt ttttagtaaa
     1021 tttaacagga ttatttatta caaaatcatt tgatgtattg agagcaaaat aatctataat
     1081 ttgagctttt gtatatccct tatctatagc attgttaagt gcaactgacg cctctgcttt
     1141 tgatttaaaa tctccataat aataatagta tgtaccattt ttattggcaa attccattac
     1201 agtaacatta tcacctaagt ttgtgaattt tttagtgtct tgttttgcta cagtatcaag
     1261 caataaaatt gaaaatctat taaattcttt aaactttaac tcatcaggac gattgtctac
     1321 aataatctcg gcatcgcctt ttgtttttag aactcgtatt tctacacgtc tattaagttt
     1381 tgctttatct gcatctacaa caccactctc ggtaacatcc attacagctt tacgagtagt
     1441 acctgctgct gtagaaacaa atcgctcgta ttcaacaccg tttttagcta aataatctat
     1501 taccgcacgt gaacgatttt ctgataattt ttgattataa tctgcatttc cgtatgaatc
     1561 ggtatgtcca ataacttcaa tatagagact tggattttcc atcataatag taagcaactt
     1621 attggcttct atcaatgcat ctctattcaa ttcatatgaa ttcaaatcaa aataaatatt
     1681 acgaattaca taatattttt tattatcaac agcttctggg tacattacag cattaacctg
     1741 aactgttttt tctactgtag ttttagcaat agacacgtgt ttttctaccg aattatattt
     1801 tggtctggta aaataaattt tatactctcc ggcaggcaca accaatgaat attctccggc
     1861 ttcatttggt gttatttttt taacagtatc tttggttgcc acatttacaa ctgttacggc
     1921 aacagtataa tcgagcggtt tattatcatc aaaactcact acaccttgca aagtaacagg
     1981 caactctttt tcttgtggtt tcacatcaag aaattcacat tgataaatat ctgaagctcc
     2041 aaacgtaaaa tatccctcat gacgagcaag tgcataaaat gcttgcttac cattcataat
     2101 aggataaaaa tatgcattat tttcaggaga atttatagga gcacctatat taacaggttc
     2161 tgaccacaca tttttagcaa tcatggtagt tttaaaaata tctaactctc ccatattgta
     2221 atgcccttca gacgaaaaat ataatgtaac accatcttca caaattatag gtgtttgctc
     2281 atcaaaagct gtatttattg ttggtcctaa atttcgaagc ggtccccaat ctccttttgt
     2341 tttttcactc acataaatat cgagaccacc aaagccacct tttctgtcac tggtaacata
     2401 caaacgattt ccatcgggtg atatcattgc atgtgtttca ttatttacag aactaatatt
     2461 tttattaaat tttttcattg gtgtccatgg atcttttctt gactttcgag tactataata
     2521 taaagtaccg gtagaaatat cggtcatacc accattttcc catgtgtttt tatacaatat
     2581 taaaaaagtt ccatcagcat ttacactcac agtattacat aaaccgcctg tttttaattg
     2641 atccgaaatg tcttttggtt ctgtccactt atcatcaccc agcgattcgg tatacattat
     2701 tttatatacc tttttattgg catcatcttc accttttttt gttgtttgtt ttatttctac
     2761 caatcgagta taaaacaata attttccatc agatgtagga catggattaa attcatccca
     2821 tgctgaattt atagtatttc ccacattaaa tatttcaact ggcacaggat gtttggaaat
     2881 ttccttagca tttctacacg attgcaattg gcgttgtgct atttgaaaat ggtaattatc
     2941 agatattggt aaagtatcga tatatcgttg atacatagcc aaagcttcgt catagtattt
     3001 attggtatga tatgcataag ccaaatgata cattgcttgt aacggcgctt ttttctcggt
     3061 aattgaacct tctacatagg cttttgaaat accggttaca gctttttgta aatatggaat
     3121 tgaacgtcct gcttcaaatg gcatacgttg ataacataat cctattttat aatttacatt
     3181 aaaattttta ggatccattt ttaaaatgtc tttgtacatt ttaagggctt gttcataatc
     3241 ctcgtttaaa aagaaatatt cagcatcttg cagtttttgt cgcaattgcg gtgattgtgc
     3301 atttgcaaaa aatactgaaa aaataaagaa aattgaaata ataaagtatt tcattatatt
     3361 tacatattaa aattataccc tacaaaatat tgtgttcaaa gatataaaat attgattaaa
     3421 taaaacaatt tttacaaaaa atgaaactat tttttttata acaacgtatg aatggttcaa
     3481 aaaataacaa taaaattcct gtttacagtt tacaatcatt tatacaaagc aatgttttca
     3541 actcatactt tcaagttgaa ccatttgata caaacaggca ttttcaagta gcatacccac
     3601 accgtcatga tttttacgaa gtattatttt tacaaaaagg ttctggattt catataattg
     3661 attctaatac atatactatt aatcctccct gtgttttttt tctatctccc ggacaagctc
     3721 acaaacttga attatctcaa gatattgatg gatttatttt cttatttact accgaatttt
     3781 atgctttatt tgactttaat aaaaataaat tattagagtt cccattcttt ttttctgttg
     3841 aacaatcaaa tccgccttta tatataacaa actcatcgga ctttacattt ttaaaagaat
     3901 tatttgctcg tgcatataat gaatccgcag gtaatattac aaacatggaa gttattcaca
     3961 ctattttaca acttatttta ctaacctgca atcaattata tactaaaaac actgctacat
     4021 ataccccaaa aaaaagtcaa attattgtaa aaaaattttt actggcatta gaagaaacct
     4081 acatttacaa tcattctatt gagtattatg caaaacaatt agctattacg ccaaatcatc
     4141 tcagtcaaat agtaaaacaa ataaccggaa aatcaacaag ttctattatt caagaaaaac
     4201 gattaattga aattaagcga ctatttcttc ataccgatat gagcatagga cacatagccg
     4261 aacatctcca ctttaatgac atttcgtata tgactaaatt ttttaaaaaa tacatgaaaa
     4321 ttaccccttt attttacaga gaaaatgagc ttaaaaaagc taatatgaaa taattagaca
     4381 ctatccatac tatttgttaa aaaacaaaaa tccatgaaaa ataccaaaaa aaagtaatat
     4441 tttcatttat aattatcaaa aaaaagtgtt gctttgtagt ctaaaatcat tcagttgatt
     4501 gtattactag taataacatt aaaacaataa taaacatggc aaattatttt aattcattac
     4561 cattccgtct tcaaaaaatt gaattaggaa agtgtgattt tatggacagc tcagaatttg
     4621 ataaaggagt tgagtacatt aaaggcaaaa aaattgtaat tgtaggatgt ggtgcacaag
     4681 ggttggcaca aggtcttaac ttacgcgaca gcggacttga tgtgtcgtat gcattacgta
     4741 aaatagaaat tgatgaaaat caaatttcat atcaaaatgc aaaaaacaat aactttgtag
     4801 tagatactat cgaaaattta gttcctcaag gtgacttaat attaaattta actcccgata
     4861 aataccatac tcctgttgta aatcatatca tgcctttaat gaaaaaagga gcatgcttat
     4921 catattctca cggttttaac attgtagaag aaggaatgca aatacgtaaa gacattacgg
     4981 taattatggt tgctccaaaa tctcctgctt ctgaagtacg tgcagaattt ttacgtggtt
     5041 ttggtgtgcc tacgttaatt gctgttcacc gcgaaaatga tcctaacggt gatggtttag
     5101 aaatagctaa agcatactgc gttggaacag gtggccacaa agccggtgta ttacattctt
     5161 catttgtagc cgaagtaaaa tctgacttaa tgggtgaaca aacaatactt tgtggattat
     5221 tacaaacagg ttctattttg tgtttcaaca aaatggttga aaaaggtata gatgcaggtt
     5281 gggcttctaa atttgtacaa tacggttggg aagttattac cgaagcttta aaaataggtg
     5341 gtataactca tatgatggat cgtttatcaa atcctgctaa aattaaagct tattacttag
     5401 cagaagaatt aaaacaaata atgcgtcctt tatttgaaaa acaccaagac gacatccttt
     5461 caggtcattt ctctaaaacc atgatggaag actgggcaaa tggcgacaaa aatcttttaa
     5521 cttggcgtgc ggcaacagca gaaacagctt tcgaaaaaac tcctgctgcc gatgttgaaa
     5581 ttagtgaaca agaatatttt gacaaaggtg tacttatggt tgcatttgta aaatcaggag
     5641 tagaattagc atttgaactt atgactgagt ccggcatgaa acctgaatca gcatactacg
     5701 agtctttaca cgaaactcct cttattgcaa acaccattgc acgtaaaaaa ttatatgaaa
     5761 tgaatcgtgt aatttctgac actgcagaat acggttgcta tttgtttgac catgcttgta
     5821 aacctttatt agctgatttt atgagtaaaa ttgataccga tgttattggt aaagaataca
     5881 atgcgggcaa aaataacaaa gtagataata tggaattagt taaaataaac gaagctattc
     5941 gttaccacga tattgaaatt tgtggtgaag aacttcgtgc tgcaatgact gcaatgaaac
     6001 caattatcta atactaaaat aagaacatat aaataaggct gcttgaaaat atcaagcagc
     6061 ctttttttat tgttactaca tgcttttaat atacgggaaa atactatcaa taaatattac
     6121 ctatcaccta ttgaactagt tgatttataa agaattttga attaccaatt ctgcataaac
     6181 aattttgttt atatttgtac acggatttta taaacagggg taggcatgac acacgacgat
     6241 gtattattag cactgaatga aacagcggtt tcacaagaat tacaaacaat agctcataaa
     6301 gttttacaga accaacgaat cactactcaa gactgcttat tattgtatga acaaggagaa
     6361 cttgcttttg taggtgcttt agccgatttt gtgcgttttc aaaaaaacga aaatatagta
     6421 tttttcaaca aaaatattca tatagagcct acaaatatct gcattcacaa ttgcacattt
     6481 tgttcatatt ccaagaaaaa aggtgaccct ctctgttggg aaatgtcaat agacgaaatg
     6541 cttgcaataa ttaccaaaat tccggaacat gcaataaccg aagtgcatat agttggtggt
     6601 gttcatcctg acagaggtat tgatttttat tgtgatttgc ttactgctat aaaaaacata
     6661 cgaccttcta tccaaatcaa agcatttaca gcagttgaaa ttgattttat ggcaaaaaaa
     6721 agcggttttt ttgtttcaga aattcttcaa aaattaaaag acgcaggact acaaactatg
     6781 cccggcggcg gtgccgaaat ttttgacgaa tcattacgca aaattatttg tcctaccaaa
     6841 acatcgagtg aacgttggct caaaattcat gaaactgcac ataaaacagg aattccaacc
     6901 aatgctacaa tgctatatgg tcatattgaa aaatttgaac acagagttga ccatttaaac
     6961 agattacgaa atttgcaaga taaaacaaat ggttttcaag catttatacc ccttaaatat
     7021 aaaaaagaaa ataattattt aggtattgaa cacgaagttc cgtggagtga agatttacga
     7081 aattttgcta taagtagaat atttcttgat aattttaaaa atataaaagc atattggcct
     7141 atgttgggca aagagctttc tcaaatgaca ttggaatttg gtgttaacga ttttgacggt
     7201 actataaacg attctacaaa aatatattct atggcaggag cagaagatac tcaccctacc
     7261 ctaaccgaag agcaaatggt agcattaata catgccgttg gacgaaaagc tatagaacga
     7321 gattctgtat acaatatcat acaaactcac taatctacat gaacagtact tggatacaca
     7381 ctctatggaa acctctgtac aataaaataa tacttattat tgtttgtggt attattcctc
     7441 ttataattta taacaaatgg gatattgtac taattataaa caaatattgt acaaatgacc
     7501 tgcttgatgt attcttcatg tattatacaa acactgcatt aggtggaata tttgttatat
     7561 gtacttgtat ttgtgtattc tactcgtatc gcaaagcttc aatgattgca ttaagcggaa
     7621 ttataatttt gataacaagt gtagtattga aacagattat ttttcaagga taccctcgtc
     7681 caacagcaga aatacctcat aatcagtttt ttcacataat tgatggattt acatacgctc
     7741 gtcataattc atttccctc
//
LOCUS       DAOTOW010000010         7355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_8847_length_7355_cov_3.728493, whole genome shotgun sequence.
ACCESSION   DAOTOW010000010 DAOTOW010000000
VERSION     DAOTOW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8847_length_7355_cov_3.728493"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="P5243_00390"
     CDS             complement(<1..1771)
                     /locus_tag="P5243_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17933.1"
                     /translation="MKCNLLFAILYIFMFFSAYGQKIENFSNAKLPTSYSTGKFVGTD
                     SIVWNYVNARGGQTTTANANAAISLNKAANACLFSDTLRYGLQSIAFQYEQELTTSCN
                     ASVWVNDSCIALLTTAGEADVTKYVRIEHLSFTGNVVITIKQNAANSGQITIDDIALV
                     FAEKPVLPFTITTIEHNSKSTRIVFSHSLLSVHVSMEPHNQILALNTIDSVLTITYNQ
                     QLCGVYTVALSQMYDFIGRSCRDTLFTVEFEHVPQVHDIRITEIMADPSPLVALPEFE
                     YIELFNASECDINLVYLSLAVGNELYALPHCIVKSGSYVLLVSQKAKYLYGDTVTVCS
                     MSSFPALLNSGQTIALMQAHAVISSVTYNDSWYGSKIKKDGGWSLEKIDINNVSETQG
                     NWCAANNFRGGTPGYSNSVSAVNIDTQNPKIIYMALLNDTTILCKTDENCNPDIVLQS
                     LECSDDIQIYSCKSVNNNLQEFILYSNKPFHKQNVYTCVVTDNLCDFAGNCADPHQFE
                     LALYDTVCNRNEILITEFLHNPIPGRNDFVELYNNSNKFINLSHIIIANRDSVRNDVG
                     SYCIVTQNPIVFLPHTYTVLSVDA"
     gene            1896..2912
                     /locus_tag="P5243_00395"
     CDS             1896..2912
                     /locus_tag="P5243_00395"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRS17934.1"
                     /translation="MKIAIVGVSGAVGQEFLRILEERNLPMEELVLFGSARSAGSTYT
                     FKGKTLTVKELKHNDDFKDITIALASAGASTSKEYAETITKYGAIMIDNSSAFRMDDD
                     VPLVVPEVNAADAKNAPRNIIANPNCTTIQMVVALKALENLSHITRVHVSSYQSTSGA
                     GAMGMAELEQQVQDYAKGNPLKVEKFAHQILFNVIPHIDVFTDNGYTKEEMKMYNETR
                     KIMHSDIQVSATTVRVPTMRAHSESIWAETVTPVTPEAFRAECEKTKGITVIDNPANK
                     EYPMPLYLAGKDDVYVGRIRKDLTNPNGITFWCVSDQIRKGAALNAVQIAEYLIAEGA
                     IKTK"
     gene            3053..3802
                     /locus_tag="P5243_00400"
     CDS             3053..3802
                     /locus_tag="P5243_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRS17935.1"
                     /translation="MEALFLHKTIRKFKDTPIPNEVIQQILLAAIRASNTGNMQLYSI
                     IVTTDATIKQQLCEQAHFNQAMVKQAPVILTFCADLHRFTQWCTLRNAEPYYDNFLSF
                     YTATIDAVIAAQNACIAAESIGLGICYLGTTNYNTQQIIDILQLPKLVVPVTTVVIGY
                     PDEQPPLTHRLPVEAVIHNQTYTCPNNTTIEQWYAEKENLEESKKFVAENNLDNLAQV
                     FTKKRYTAQNNTVFSQKLLDILNKQHCMNNQ"
     gene            3814..4278
                     /locus_tag="P5243_00405"
     CDS             3814..4278
                     /locus_tag="P5243_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRS17936.1"
                     /translation="MIQRIQSLYLLLSAIVTAISLGMPFLQCSAPGSDVILYAFRISD
                     SSSLIFSEHTPFYTLGASVSTSILLSVITIFLYTNRSLQMRLTIYGIILKCAILAVIG
                     YVWYMLNSIEIQFTIIPQIGIICIAIAIVLEWLAYKGIQKDEKLVRSIDRIR"
     gene            4301..5854
                     /locus_tag="P5243_00410"
     CDS             4301..5854
                     /locus_tag="P5243_00410"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRS17937.1"
                     /translation="MKRILLSLAVLCIISTTFAQNKMRVEVQVKGLQDTTLILGYHYG
                     SKKLVADTIRVDSKGKGVFKRDTLMDAGLYLVLTPNMRYFEIILDKDQEFKVTTDTAD
                     LFTNLKFEGSVDNQVFLDYQNKSSDLYKKRKPVFDKLKWYYDIKDTTIYSKKQREIQR
                     DSIKILQNQLETIRKEMIAYEDGIIKAYPTSLLSSILLCTRELDIPDYPRNEKGEITD
                     SLFKYTYMKNHYFDKVQLGDSRLLRTPVYEMKIENYFDRELLQIPDSIIPEVDKLMGK
                     ILEQEKKGYEGKMYYYTLHHMFMKYQNPKYMGLDNIFVYIMEQYYLKKKLPAKVIADT
                     AYMAKIEKRYNEMSKNRIGIKAPNFVAYSNKDEWMQLNSIQADYIVLYFWDTDCGHCK
                     KTIPEWHDLYVQNEFKKKGVACILFYTQTEMEQWKKFIVDKGLDDCINVFDPYQNTNF
                     RQNYDIYSTPVSYVLDKNKKIIAKRIPPETIVDLLNHELERNKKTQNTTNQKQEQPAP
                     NQKNTSQKK"
     gene            5925..7061
                     /locus_tag="P5243_00415"
     CDS             5925..7061
                     /locus_tag="P5243_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS17938.1"
                     /translation="MIQEIKKNPMYTYLMVLVICATAGLQGWMALFTNFAKEIVHVNG
                     LQIGISQSVREIPGFLTFLAIYLLLWFKEHKLSAWSVIVMGLGIAITGYLTTFDGLLL
                     STLIMSIGFHYFETTNKSLTIQYFNKQQSATVFAKLRGLGAFANVLIGIYVFFVSQYV
                     SYQTNFLCLGILVICIGIYSLMQNPVKKEIPPQKNKLVLKKAYWLYYVINFLSGARRQ
                     IFIVFAIFLLVEKYELSVSHVALLFIINNTITYFANPIISKAINTYGERVVLTLESVS
                     LCILFLGYAFINNVWIVAGLYVIDNFFFNFSIGINTYLQKIAAPRDIAPSTAVGFTIN
                     HISAVVIPIIGGSLWMISPMLPFIIGALLAIVNVWFIQKISIHT"
BASE COUNT         2696 a         1252 c         1127 g         2280 t
ORIGIN      
        1 tagcatctac cgaaagtaca gtataagtat gaggtaaaaa cacaatcgga ttttgtgtaa
       61 ctatacaata agaaccaaca tcgtttctta cactatctct attagcaatt ataatatgtg
      121 ataaatttat aaacttatta gaattattat acagttctac aaaatcattc ctaccaggta
      181 taggattatg taagaattct gtaattagta tttcatttct attacatact gtatcatata
      241 atgccaattc aaattgatgt gggtctgcac aattccctgc aaaatcacat aaattatcag
      301 taacaacgca agtataaaca ttctgtttat gaaatggttt atttgaatac aatataaatt
      361 cctgtaaatt attattcaca gatttacaag aatatatttg aatatcatca ctacattcca
      421 atgattgtaa cacaatatca ggattacaat tctcatcggt tttacacaat atagttgtat
      481 cattaagcaa agccatatat attatttttg gattttgagt atcaatattc acggcagaaa
      541 cagaattgga atatcccgga gttcctcccc taaaattatt tgctgcacac cagttacctt
      601 gtgtttcact tacattgttt atatctattt tttccaaaga ccatcctcca tcttttttaa
      661 tttttgaacc ataccagcta tcattatacg taaccgatga aattacagca tgagcttgca
      721 taagagcaat tgtttgccca ctattgagca aagcagggaa cgacgacatg gaacataccg
      781 taactgtatc gccatacaag tatttagctt tttgcgaaac tagcaataca taggaacctg
      841 attttactat acaatgcggt aacgcataca attcattacc aactgcaagc gatagatata
      901 caagatttat atcacactct gatgcattaa ataattcaat atattcaaac tcaggtaatg
      961 ctaccaatgg tgaagggtct gccataattt cggttatacg tatatcatga acctgaggaa
     1021 catgctcaaa ctctactgta aataaagtgt ctctacatga ccttccaata aagtcataca
     1081 tttgcgaaag tgctactgta tacactccgc acaattgctg attatatgtt atggtaagaa
     1141 cagaatcaat agtatttaat gcaagaattt gattgtgtgg ttccattgac acatgtaccg
     1201 atagaagaga atgtgaaaaa acaatacgag ttgatttaga attgtgctct attgttgtta
     1261 ttgtaaatgg taataccggt ttttcagcaa acaccaacgc tatatcatct atagttattt
     1321 gtccagaatt tgctgcattt tgcttaattg taattacaac atttcctgta aatgacaaat
     1381 gttcgatacg cacatacttg gtaacatcag cttctccggc agtagtaagc aaagcaatac
     1441 aactgtcatt tacccaaaca cttgcattac aacttgttgt aagttcttgc tcatattgaa
     1501 atgctataga ttgcaatccg tagcgcaacg tatcagaaaa taaacacgca ttcgctgctt
     1561 tatttaaact tatagctgca tttgcatttg cagtagtagt ttgtcctccg cgagcattta
     1621 cataattcca tacaatactg tcggttccta caaatttgcc tgtagaatat gatgtaggga
     1681 gtttagcatt cgaaaaattt tcaattttct gaccatacgc agaaaaaaac atgaaaatat
     1741 ataggatagc aaataaaagg ttgcatttca taattgtaac atatattttt gttgcgactt
     1801 tgatggggta aaaataacaa aacaacacaa ataaacgtgt gtaaccatca aaaaaagtga
     1861 aacattaata cattttacaa taaattatta catctatgaa aattgcaatt gttggtgtca
     1921 gcggagcagt tggccaagaa tttttaagaa ttctcgaaga aagaaatctt cctatggaag
     1981 aattagtatt atttggttcg gcacgtagtg caggaagcac ctatacgttc aagggcaaaa
     2041 cacttaccgt aaaagaacta aaacataacg acgattttaa agatattact attgcattag
     2101 cttcggcagg tgcaagtaca tcaaaagaat atgcagaaac tattacaaaa tacggtgcca
     2161 taatgataga caactcaagt gcctttagaa tggacgacga tgtaccgtta gtagttcctg
     2221 aagtaaatgc tgcagatgct aaaaatgcac ctcgcaatat tatagcaaat ccaaactgta
     2281 caactattca aatggtagtt gcgttaaaag cattagaaaa tctttcgcat attacacgag
     2341 tacatgtttc atcataccaa tctaccagcg gtgccggtgc tatgggtatg gccgaactgg
     2401 aacaacaagt tcaagattac gctaaaggaa atcctttgaa agtagaaaaa tttgctcatc
     2461 aaattttatt caatgtaata ccccatattg acgtatttac cgataacgga tacactaaag
     2521 aagaaatgaa aatgtataat gaaactcgta aaattatgca ttcagacatt caagtaagtg
     2581 ctactactgt tcgtgtacca accatgcgag ctcacagcga atctatatgg gcagaaacag
     2641 ttacaccggt tactcccgaa gcgtttagag cagaatgcga aaaaacaaaa ggaatcacag
     2701 taattgataa ccctgccaac aaagaatatc caatgccatt gtatttagct ggcaaagacg
     2761 atgtatatgt tggtcgtatt cgtaaagatt taacaaatcc taatggaatt accttttggt
     2821 gtgtaagcga tcaaattaga aaaggagcag ctcttaatgc agttcaaatt gcagaatatt
     2881 taattgcaga aggtgctatc aaaacaaaat aagtttatta tacatattta caaatgcctc
     2941 taagacaaat cttagaggca ttttttttac tatacaatca ttataatttt gcccctctta
     3001 cccttaagct ttaacataat ttattatttt tgttaccata aaataatact ctatggaagc
     3061 actgttttta cacaaaacta ttcgaaaatt caaagacaca cctataccaa atgaggtaat
     3121 acaacaaatt ttacttgctg ctattagagc atcaaataca gggaatatgc agctatattc
     3181 aataattgta accacagatg ctactataaa acaacaatta tgtgaacaag ctcattttaa
     3241 tcaagctatg gtaaaacagg cccctgtaat tcttacattt tgtgccgatt tgcatcgctt
     3301 tactcaatgg tgtacactca gaaacgcaga accttattat gataattttc ttagttttta
     3361 tactgccacg atagatgctg taatagctgc acaaaatgca tgtattgccg ctgaatcaat
     3421 cggattgggc atatgctatt taggtactac taattacaat acccagcaaa taattgacat
     3481 actacaatta cccaaacttg tagtaccggt aactacggtt gtaataggat accccgacga
     3541 acaaccgcca ttaacacaca gattacctgt agaagctgta atacacaatc aaacatatac
     3601 ctgcccaaac aacacaacaa tagaacaatg gtatgccgaa aaagaaaacc ttgaagagtc
     3661 caaaaaattt gttgcagaaa acaacctgga taaccttgca caagttttta caaaaaaacg
     3721 atatacagct caaaataata cagtattttc gcaaaaactt ttagatattt taaacaaaca
     3781 acattgtatg aataatcaat aatttgtact catatgattc aacgaattca gtctttatat
     3841 ttattgttgt cggctattgt aactgctata agtcttggaa tgcctttctt acaatgttct
     3901 gcacccggaa gcgatgtaat actctatgct tttagaattt ctgatagttc atctcttatt
     3961 ttttcagaac acactccatt ttacacatta ggcgcatcgg taagcacttc aattcttttg
     4021 tctgttatta caattttttt atacacaaac cgttcactgc aaatgcgatt gacaatatat
     4081 ggtataatac ttaaatgtgc aatattagca gtaatcggat acgtgtggta tatgcttaat
     4141 tctattgaaa ttcaatttac tataattcca caaatcggaa ttatttgtat tgctatagct
     4201 atagttttag aatggttagc atataaaggg atacaaaaag atgaaaaatt agtgcgttca
     4261 atagaccgaa tacgataata aactataata attcaaagct atgaaaagaa tattactcag
     4321 tttagcagta ctttgtatta tttcaacaac atttgcacaa aacaaaatgc gagttgaagt
     4381 acaagtaaaa ggattacaag atactactct tattttaggg tatcattacg gttctaaaaa
     4441 attagttgcc gatactatta gagttgacag caaaggaaaa ggcgtattta aacgcgatac
     4501 tcttatggac gccggcttat atttggtttt aacgccaaac atgcgatatt ttgaaattat
     4561 tttagacaaa gaccaagaat ttaaagtaac cacagacact gccgatttat ttacaaattt
     4621 aaaatttgaa ggaagtgtag acaatcaagt ttttcttgac tatcaaaaca aaagttcgga
     4681 tttatacaaa aaacgtaaac ctgtttttga taaactaaaa tggtattatg atataaaaga
     4741 tactacaata tattccaaaa aacaacgcga aattcaacgc gattccatca aaatattaca
     4801 aaaccaattg gaaaccattc gcaaagaaat gatagcatat gaagatggta ttataaaagc
     4861 gtatccaacg tctttattgt catcaatttt attatgtaca cgcgaattag atattcccga
     4921 ttatccacga aacgaaaaag gtgaaattac tgattcatta tttaaatata cctatatgaa
     4981 aaatcactac ttcgacaaag ttcaattagg cgacagtcga ttattgcgaa ccccggtata
     5041 tgaaatgaaa atcgaaaatt actttgatcg tgaattactc caaattcctg attctataat
     5101 tcccgaagtt gacaaattaa tgggcaaaat attggaacaa gagaaaaaag gatacgaagg
     5161 caaaatgtat tattacactc tgcaccatat gtttatgaaa tatcaaaacc ctaaatatat
     5221 gggactcgac aatatttttg tgtacataat ggagcaatat tatttgaaga aaaaattacc
     5281 ggccaaggta atagccgata cagcttatat ggcaaaaatt gaaaaacgat acaatgaaat
     5341 gtctaaaaac cgaatcggaa ttaaagctcc taactttgtg gcatacagca ataaagatga
     5401 atggatgcaa cttaattcta ttcaagccga ttatatagta ctgtattttt gggataccga
     5461 ttgcgggcat tgtaaaaaaa ccattcccga atggcacgac ctctatgttc aaaatgaatt
     5521 caagaaaaaa ggtgttgcct gcatattgtt ttacacccaa acagaaatgg aacaatggaa
     5581 aaaatttatt gttgataaag gtttagacga ttgtataaac gtatttgacc catatcaaaa
     5641 tacaaacttc cgtcaaaatt acgatatata cagtacaccg gtttcgtatg tattagacaa
     5701 aaataaaaaa attattgcta aacgtattcc accggaaact attgttgact tgttgaatca
     5761 cgaattggag cgaaacaaaa aaactcaaaa cacaacaaat caaaaacagg aacaacctgc
     5821 tccgaatcaa aaaaatacaa gtcagaaaaa ataaaaaaac tatacgaata tttacaccct
     5881 aaaaaagccg tatatgtgcg gcttttttta ttacacaacc atatatgata caagaaataa
     5941 agaaaaaccc tatgtacacg tacctcatgg ttttggtaat atgtgctaca gcgggtttac
     6001 aaggttggat ggctcttttt accaattttg caaaagaaat tgttcatgta aatggtcttc
     6061 aaataggtat atcgcaatct gttcgcgaaa ttccgggctt tcttaccttc ttagcaatat
     6121 atttactact atggttcaaa gaacataaac tttcggcatg gtcggtaatt gtaatgggtt
     6181 taggaattgc aatcacaggt tatttaacta cattcgacgg acttttactt tcaacactta
     6241 taatgtctat agggtttcat tactttgaaa ccaccaataa atcacttaca attcaatatt
     6301 tcaacaaaca acagtcggct acagtatttg ctaaacttag aggattagga gcctttgcaa
     6361 acgtacttat aggcatatat gtattctttg tttcacaata tgtatcatat caaacaaact
     6421 ttttatgttt aggcatttta gtaatttgca taggaatata tagcctcatg caaaatcctg
     6481 taaaaaaaga aataccgcca caaaaaaata aattagtact aaaaaaagca tattggttat
     6541 attatgtaat caattttttg agtggtgccc gacgacaaat atttatagta tttgcaattt
     6601 ttctattggt tgaaaaatat gaacttagtg taagtcacgt tgcattgtta tttataataa
     6661 acaataccat aacatatttt gcaaacccta ttatatcaaa agctataaac acatatggtg
     6721 aacgggtagt tttaacttta gaaagtgtaa gtttatgtat attatttttg ggctatgcct
     6781 ttataaacaa tgtttggata gttgcgggat tatatgttat tgataatttt ttctttaatt
     6841 tttcaattgg cataaatacc tatttacaaa aaatagcagc tccccgcgac atagcaccct
     6901 ctactgcagt tggctttact attaatcata tttctgcagt agtaattcct attataggag
     6961 gttctttgtg gatgataagc ccaatgttac cttttataat tggagcattg ttggctatag
     7021 taaacgtatg gtttatacaa aaaatcagta ttcacacata atcagaaccc accttacctc
     7081 attatttaaa acctgtaaac taaagataac acagaaaaaa attcattctg tgaaaatctg
     7141 tggaatctgt ggtaaaaaaa tgtagctaaa aaatagttca ccacagatag cacagataac
     7201 ctcagattaa tacaaaaagc tatttgtgaa aatctattgt aaaaaacatt cctatttttc
     7261 tcttcatcat aataatctta accatagatt acacaaaaaa tcattctgtg aaaatctgtg
     7321 gaatctgtgg taaaaaaatg tagctaaaaa atagt
//
LOCUS       DAOTOW010000011         7178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9098_length_7178_cov_3.238944, whole genome shotgun sequence.
ACCESSION   DAOTOW010000011 DAOTOW010000000
VERSION     DAOTOW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9098_length_7178_cov_3.238944"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="P5243_00420"
     CDS             <1..364
                     /locus_tag="P5243_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRS17939.1"
                     /translation="LQTRFNKHNFEELSENDDFICISAKHKRNIDSLIQALLQEVHYG
                     EISQDDAILTNMRHYEALQQAHIAITHVLQGIDTGLSNDLLSLELRQVLHYVGLITGS
                     IGTEEMLGYIFEHFCIGK"
     gene            402..554
                     /locus_tag="P5243_00425"
     CDS             402..554
                     /locus_tag="P5243_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17940.1"
                     /translation="MAKTMCKLAKELPDSLEKIVGIAENYAFICKKCGRVAHVEERLC
                     KPISVK"
     gene            complement(558..1595)
                     /gene="lpxD"
                     /locus_tag="P5243_00430"
     CDS             complement(558..1595)
                     /gene="lpxD"
                     /locus_tag="P5243_00430"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRS17941.1"
                     /translation="MEFTVLEIANFLQGTVEGDAQAKVSKLSKIEEGEPGSVSFLANP
                     KYTPYIYTSKATAVICNKTFVPEQEITTTLIRVDDAYSSFAKLLDLYTKQQQTKKPKI
                     SRKANIHRKAKIGKQVSIGDFVSIAEDVTIGNNTIIHPNVTIYKGVTIGENCLIYAGV
                     RILDNCIIGNNCILQPGAIIGSDGFGFAPIENGKYYKIPQTGNVILEDDVEIGANATI
                     DRATMGSTIIRQGTKIDNLIQIGHNCEIGSNTVIAGQTGISGSTKVGSNCMIGGQTGV
                     AGHLKIGNNVKIAAKSGIMMDVPDNDFQMGAPSFNAREYKKSYIHFINIDKLAKRINQ
                     LEKELKDLKKE"
     gene            complement(1689..2462)
                     /locus_tag="P5243_00435"
     CDS             complement(1689..2462)
                     /locus_tag="P5243_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17942.1"
                     /translation="MDDKKNNIYGIVGTILFHTIIIVILVLFAFKAPTVEFPEPNGIA
                     ITFGEEIQGDGQIKPDEIAPQPQEEQIPQKTSTSASEDLDVTTNDNSDAISVRKTTKP
                     TETKRELTQEEKEQLEREKAAEEFRKKQEKLFGSNFSNNPGNSNDGKEDGVQGDKAGS
                     STNKNSKGSPGNPLGNKDAVYLAKPLNTTNCNKPIELTVKINSQGNVVAITDIETALS
                     EQSCIEAAKTAAKQNRFVSDPTREVRYAKITYDYTVSQR"
     gene            complement(2469..2858)
                     /locus_tag="P5243_00440"
     CDS             complement(2469..2858)
                     /locus_tag="P5243_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS17943.1"
                     /translation="MAIVSRNKVSTEFNMSSMTDIVFLLLIFFMVTSTQISPNGLQVS
                     LPKSSNQVTPNQRTSVSITPDLQFAIEKEIVPFEQLESALIAKLQGEQEPTILLRVDK
                     DVPTGETVKVMDIANRNKWKFVLATKP"
     gene            complement(2861..3583)
                     /locus_tag="P5243_00445"
     CDS             complement(2861..3583)
                     /locus_tag="P5243_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRS17944.1"
                     /translation="MNITSLLFGFVQVTLDGSGANNSSANVIINATPEELTFWNMTMK
                     GGWIMIPIFLLSILAVYIIVERYFAIQKAEKEDENFMREIKMYIQQGKIEEARIRCKN
                     NTSPVALMIDKGISRIGKPLADVREAIENVGKLEVFKLEKRLPLLATVSGAAPMIGFL
                     GTVLGMIRAFYDMANAGGNFSISMLSNGIYTAMVTTVAGLIVGVVAYLGYNLLIAKVN
                     KVVNKLELRATEFMDLLNEPTE"
     gene            complement(3730..5817)
                     /locus_tag="P5243_00450"
     CDS             complement(3730..5817)
                     /locus_tag="P5243_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17945.1"
                     /translation="MSVLTEYSLWFLPICIAGGLSVALLLYYRSAANQIIPKHIIQIA
                     FVLRTIFISILLFLLLGPFIEQTKRSIVKPTIVFLQDNSNSIVMHNDSLYYKTTYKTN
                     VEQLTKALSNNYATHTYTFGEHISDSSLITYNQKRTNISNALQQIYSRYFNANLGAII
                     LASDGLYNTGDNPLYSPIFESHIPIYTIALGNPNQVRDNVISDVQHNSVVFKNIPFTA
                     RVFIESHNLQGKKSTITILEGSKQVFQTNIQSNSNTYVTSIDCKLTTQSLGQVLYTVR
                     IENHDNEITYKNNTFLFSVDVMESQQNILILYESVHPDVAAIRRALETNKNYSVTVSS
                     TQEFAGNINNYNCIIFHGLPQSYGKSKELVEKAKSFSIPALYTYNYTMNLALLDILNV
                     GLSIRQRSNTADEVQALYNTENTIFDIDQESKELIESAPPLFAPYGEYVSNIQSRVVL
                     WQRMQQIQTQRPLLQFFNTNGYKTAIFTGEGLWRWRIHAYKKNKSFTAFDVLINKCVN
                     YLALTQKQELFRVQTKPMITENQDIIFRAELYNKSYEPIPNKEIRLRIWNQKGKEFPY
                     VFTSNNDYYSLQVGKMPIGTYSYTATVEIEGSKYEQKGSFMILPLQLEYTQTRANHAL
                     LQTISLQTGGSMLQKEQMQQIYDSIQSNKNIVPIAYTSKTRTLLLDSFLLLITLIVFA
                     GTEWFLRKFYGSY"
     gene            5986..7071
                     /locus_tag="P5243_00455"
                     /pseudo
     CDS             5986..7071
                     /locus_tag="P5243_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
BASE COUNT         2287 a         1070 c         1180 g         2641 t
ORIGIN      
        1 attgcaaaca cggtttaata aacataattt tgaagaattg tcagaaaatg atgattttat
       61 atgtatttcg gcaaagcata aacgtaatat tgatagtctt atacaagcat tgttgcaaga
      121 agtgcattat ggtgaaatat ctcaagatga tgctatattg acaaatatga ggcactatga
      181 agctcttcag caagctcata ttgctattac tcatgtgttg cagggaatag ataccggttt
      241 atcaaatgat ttgttaagtt tagaactaag acaagtactg cattatgtgg ggcttattac
      301 aggcagtata ggtacagaag agatgcttgg gtatattttt gagcatttct gtataggtaa
      361 gtaatgaatt cttatttttt gcgtctattc ataaaaaaac tatggcaaaa actatgtgta
      421 aattggcaaa agaattaccc gattctttag aaaaaatagt tggaatagca gaaaactatg
      481 catttatttg taaaaaatgc ggtagggtag cacatgttga agaacgtttg tgtaaaccaa
      541 tatcagttaa gtaatagtta ttcttttttt aaatctttaa gttctttttc taattgattg
      601 atacgtttgg cgagtttatc aatatttata aaatgaatgt acgatttttt gtactctctg
      661 gcattaaaac taggggctcc catttgaaaa tcattgtcgg gaacatccat cattatgccc
      721 gattttgcgg caattttaac attgtttcct atttttaagt gtccggcaac tccggtttgt
      781 cctccaatca tacaatttga gccaacttta gttgagccgg aaatacctgt ttgtcctgca
      841 attacggtat ttgaacctat ttcgcaattg tgtcctattt gaattaaatt atcaattttg
      901 gtaccttgtc taattatggt agaccccatt gtagctctat ctatagtagc atttgcacct
      961 atttctacgt catcttcaag aattacattc cctgtttgtg gaattttata atatttacca
     1021 ttttcaatag gagcaaatcc aaaaccatca gatcctatga tagctccggg ttgtaatata
     1081 caattgttac caattatgca attatctaat attctaactc cggcataaat taagcaattt
     1141 tcacctatcg taacaccttt gtaaatagtt acattcggat gaataatagt gttattacct
     1201 atggttacat cttcggcaat tgaaacaaaa tcgcctattg aaacttgttt cccaattttt
     1261 gctttacgat gaatatttgc tttacgcgaa atttttggtt ttttggtttg ttgttgtttg
     1321 gtatataaat ctaatagttt tgcaaatgaa gaatatgcat cgtctacacg aataagagta
     1381 gttgtaattt cttgttcggg gacaaatgtt ttattacaga ttacagcagt agcttttgag
     1441 gtatatatat atggagtata tttagggtta gccaaaaatg aaactgaacc gggttcacct
     1501 tcttctattt ttgataattt tgatactttt gcttgagcat caccttcaac ggtaccttgt
     1561 aaaaaatttg caatttctaa tactgtaaat tccataaaat atagggtaac ataataatgc
     1621 agtaaaatta tgatatttta tattcataat gtataatata gtgcataatt ttaatgaaaa
     1681 tattaaaatt atcgttgtga tacagtatag tcataggtta ttttggcata tcttacttcg
     1741 cgggttgggt ccgatacaaa acgattttgt tttgctgcag ttttggctgc ttctatacat
     1801 gattgttcgc ttaaagcagt ttcaatatca gtaattgcca ccacattacc ttgggaatta
     1861 attttaacag ttaattctat aggtttgtta caatttgttg tattcagcgg ttttgctaaa
     1921 tatacagcat ctttgttgcc taaaggattt cccggagaac ctttagaatt tttattggta
     1981 ctactgcctg ctttatctcc ttgtacgcca tcttcttttc cgtcgttact gttacccgga
     2041 ttgtttgaaa aattacttcc aaataatttt tcttgtttct tacgaaattc ttcggcagct
     2101 ttttctcttt ctagttgttc tttttcttct tgtgttaatt ctcgtttagt ttcggtaggt
     2161 tttgtagttt ttcttacact tatggcatct gagttgtcat tggtagttac atctaaatct
     2221 tcagatgcag aagtacttgt tttttgagga atttgttctt cttgtggttg aggagcaatt
     2281 tcatcgggtt ttatttgtcc atcaccttgt atttcttcac caaaagttat ggcaatacca
     2341 ttaggttcag gaaattcaac cgtaggtgct ttaaatgcaa aaagtaccaa aataactata
     2401 ataatagtat gaaacagaat tgttccaact ataccatata tattattttt tttatcatcc
     2461 atatactatt atggtttagt agctaataca aatttccatt tatttctatt tgcaatatcc
     2521 attactttta cagtttcgcc ggtaggtaca tctttgtcaa ctcgtaacaa aatagttggt
     2581 tcttgttcgc cttgtaattt tgctataagt gctgattcta actgttcaaa tggtacaatt
     2641 tctttttcaa ttgcaaattg caaatcgggg gttattgaaa ctgatgtgcg ttgatttggt
     2701 gtcacttgat ttgaactttt gggtaatgac acctgtaagc cgttaggtga aatttgtgta
     2761 gatgtaacca taaaaaatat gagtaacaaa aatacaatgt ctgtcattga cgacatgtta
     2821 aattctgtgc ttactttatt tcttgatact atagccataa ttattctgtt ggttcgttaa
     2881 gtaaatccat aaattcagtt gcacgcaatt ctaatttgtt tacaactttg tttactttag
     2941 caattaataa attatatcct aaatatgcaa caacacctac tataagtcct gcaacagttg
     3001 ttaccattgc agtatatata ccgtttgaaa gcattgaaat actaaaatta cctcctgcgt
     3061 ttgccatatc ataaaatgcc cgaatcatac caagcacagt tcctaagaac cctatcatag
     3121 gtgcagcacc tgatacggtt gctaataatg gtaatcgttt ttcgagtttg aaaacttcaa
     3181 gttttccaac attttcaata gcttcgcgca catcggcaag tggtttgcct atacgtgaaa
     3241 ttcccttgtc aatcattaat gcaacaggag atgtgttgtt tttgcaacga atacgagctt
     3301 cttctatttt gccttgttgt atatacattt ttatttcacg cataaagttt tcgtcttctt
     3361 tctcagcttt ttgaatagca aaatagcgtt ctacaataat atatacagct aaaattgaca
     3421 ataaaaaaat gggtatcata atccaaccac ctttcattgt catgttccaa aaggttaatt
     3481 cttccggtgt tgcatttata ataacatttg ccgaactgtt atttgctccg cttccatcaa
     3541 gggtaacttg aacaaatcca aataataatg acgtgatatt catagatata agtttatgtt
     3601 aaaattttgt gcaaaataat tcatttttaa aaccaaaaaa aatattatgc atattgctta
     3661 ttgacaagtt ataaacgatg ctttatatac acttagtata caaatatgtt gttgtattca
     3721 aaaatatgac taataactgc catagaattt tcgtaaaaac cattctgttc cggcaaatac
     3781 tataagagta ataagcagta aaaaactatc gagcaataat gttcttgttt tagatgtata
     3841 tgctataggt actatatttt tgtttgattg aatactgtcg tatatctgtt gcatttgttc
     3901 tttctgtagc attgaacctc ctgtttgtaa tgaaatagtt tgaagtaatg catggtttgc
     3961 tcttgtttgt gtatattcta attgtaacgg taatatcata aacgagcctt tttgttcata
     4021 cttagatcct tctatttcaa ctgtagccgt atatgaatac gtacctatag gcattttacc
     4081 tacttgtaat gaataataat cattgttaga ggtaaataca tatggaaatt ctttcccttt
     4141 ttgattccaa atacgtaatc gaatttcttt atttggaata ggttcataac ttttattata
     4201 caattctgct ctaaaaatta tatcttgatt ttctgttatc ataggtttgg tttgaacgcg
     4261 aaataattct tgtttttgtg tcagagccaa ataatttaca catttattta ttaaaacatc
     4321 aaaagctgta aatgatttat tttttttata tgcatgtatt ctccaccgcc ataaaccttc
     4381 accggtaaaa atagcagtct tataaccatt ggtattaaaa aattgtagaa gaggtcgttg
     4441 tgtctgtatt tgttgcattc gttgccataa tactacacgc gattgtatat tacttacata
     4501 ttctccatat ggtgcaaata atgggggagc agattctatt agttctttgc tttcttgatc
     4561 aatatcaaaa atggtatttt ctgtattata caatgcttgt acttcatctg cagtatttga
     4621 acgttgacga atagacaggc ctacattaag tatgtctaac aatgcaagat tcatggtgta
     4681 attatatgta tacaaggccg gaattgaaaa acttttagct ttttcaacta attcttttga
     4741 tttaccgtat gattggggta agccatgaaa aataatgcaa ttataattat tgatattacc
     4801 tgcaaattct tgtgtagatg ataccgtaac agaatagttt ttattggttt ctaaagctct
     4861 gcgaatagca gcaacgtcag ggtgtacact ttcatacagt ataagaatat tttgttgact
     4921 ttccataaca tctactgaaa acaaaaatgt attgttttta tatgttattt cattatcatg
     4981 attttctatg cgaacagtat ataatacttg acccaaagat tgtgttgtta gtttacaatc
     5041 tattgatgtt acataggtat ttgaattaga ttgaatgttt gtctgaaaca cttgtttgct
     5101 gccttctaat atggtaattg ttgatttttt accttgtaaa ttatgtgatt ctataaacac
     5161 tcttgctgta aaaggtatat ttttaaatac aactgaatta tgttgaacat cgctaattac
     5221 attgtcacga acttgatttg gatttcctaa agctatagtg tatataggaa tgtgcgattc
     5281 aaaaattgga ctgtataaag ggttgtcgcc ggtattgtat aaaccatcag aagccaatat
     5341 aattgctccc aaattagcgt taaagtaacg agaatatatt tgttgtaaag catttgaaat
     5401 atttgttctt ttttgattgt acgtaatgag tgacgaatca gaaatatgtt cgccaaatgt
     5461 atatgtgtgt gttgcgtaat tgtttgacag tgcttttgtt agttgctcta cattggtttt
     5521 gtaagtggtt ttgtaatata aggaatcatt gtgcattaca atagaatttg aattgtcttg
     5581 caaaaatact attgttggtt ttacaatgct tcgtttagtt tgttcaataa aaggacctaa
     5641 caataaaaat aatagtattg aaataaagat agtgcgtaaa acaaaagcaa tttgaataat
     5701 gtgtttaggt ataatttgat ttgcggcaga acgataatat agtaataatg caacgcttaa
     5761 accacctgca atacatatag gcaaaaacca caaggaatat tcggttaaca cactcatgtt
     5821 tttaaaaaaa gtttgtcaaa tgtaataaaa acaatgtata atgagaacaa aactttatgg
     5881 aaacacaata aaatattgta actataataa aagagaggag atacttttag ttgttaatat
     5941 ctttcatttg ttgaattcat ataataagtg tatttttgta tgcgtatgga aaattttatt
     6001 gtatcagcac gaaaatatcg tccggtaacc tttgcttcgg tagtgggtca acattcaatt
     6061 actgttactt taaaaaattc tattgcaaat aatcatttag gtcaagcata tttgttttgt
     6121 gggccacgtg gtgttggaaa aacaacgtgt gccagaattt ttgcaaaaac aataaattgt
     6181 ttacaactca cacctcaaat agaagcatgt gatgaatgtg aatcatgtaa atcattcaat
     6241 gattcggctt cttttaatat tcatgaactt gatgctgcat ctaacaattc ggtagaagat
     6301 atacgttctc ttatagatca ggtaagaatt ccgccacaaa taggaaaata cagcatttat
     6361 ataatagatg aggttcatat gttatcatcg tcggctttta atgcttttct taaaacattg
     6421 gaagaaccac ctcgacatgc tatttttatt cttgctacaa ccgaaaaaca taaaatattg
     6481 ccaacaattc tttcgcgatg tcaaattttt gatttcaaca gaatcaaggt agaagatatg
     6541 gttgatcatt tagcatctgt tgcaaaaaat gagggtgtta ccgttgagcg agaagcatta
     6601 aatgtaattg cattaaaagc agatggggga ttacgtgatg ctttatctat atttgatcaa
     6661 gttgttagtt tttcaggaaa aaatataaca tatcaaagta ctatagcaaa tttaaatttt
     6721 ttagattatg agtacttttt taaacttaca gactatttct taaatgaaga ctatgctcaa
     6781 gcattagtat tatatgatga gattcttgat aaagggtttg acggtcataa ttttataacc
     6841 ggtttgagtt cgcatctcag agatttgctt gtaagtaaag atcaaaaaac agttcaattg
     6901 ctcgaagtgg gtgatgctat tcgtaaacgg tatttggaac aagcacaaca atgtagtgta
     6961 gaatttttgt ataaagcgtt agacttaacc ggaaagtttg atatgaacta caaagcaagc
     7021 aataataaac gcttgcatat agaattagca ttattgcagt tatgcaatat agtaaaaaaa
     7081 aaagtcctga attagttgaa cagcatattc aaaaacaagt tgtaacaacg cataacccca
     7141 ttaattcagc atatgctact acagcaaagc cttttaaa
//
LOCUS       DAOTOW010000012         7169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9107_length_7169_cov_3.327101, whole genome shotgun sequence.
ACCESSION   DAOTOW010000012 DAOTOW010000000
VERSION     DAOTOW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9107_length_7169_cov_3.327101"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(568..783)
                     /locus_tag="P5243_00460"
     CDS             complement(568..783)
                     /locus_tag="P5243_00460"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRS17946.1"
                     /translation="MRKNLNYKNALAYTNIGFQMLAIILIGVFGGQKLDDYFELHNSL
                     CTIICSLLSVFASIYLAIKDIIHMNKE"
     gene            complement(767..1162)
                     /locus_tag="P5243_00465"
     CDS             complement(767..1162)
                     /locus_tag="P5243_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRS17947.1"
                     /translation="MAKINETSSFLTNMITAGTTITGDISCNSDIRIEGSLKGNLVAK
                     GKLVIGSTGEIKGEVSCQNCDIEGVLEGKITIQELLSFKSTAKVTGDIITNKLAIEPG
                     AVFSGTCTMKNDTFVADPAKKIEVIEKKS"
     gene            1261..2130
                     /locus_tag="P5243_00470"
     CDS             1261..2130
                     /locus_tag="P5243_00470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS17948.1"
                     /translation="MIYLTPTGWKDYELIDSGNFEKLERFGTVVCVRPEPQAVWDKSM
                     TEAQWKQKATVTFQAKSSNSGEWIKHVACADSWKIQYHPLKLTCNLKFTSFKHVGIFP
                     EQAVNWDYMYAICSKHQNLNVLNLFGYTGIASVAARAAGAQVTHLDAIKQVVNWTRQN
                     MEDSGLRDIRWIIEDARKFVAREIKRGNVYQGIIMDPPAFGRGTNGEDWKLERDINAL
                     LKDVQKILDTQRYFFILNTYSLGFSAYIIENLLHGIFAQYSVPKEVGELYLQDSFSKK
                     LPLGVIGRFTNLV"
     gene            complement(2135..6904)
                     /locus_tag="P5243_00475"
     CDS             complement(2135..6904)
                     /locus_tag="P5243_00475"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS17949.1"
                     /translation="MTDSHQYQSDECIELHKRIEILQQHILSMRNTYSNNYFETLIED
                     MPLAYHSLDEYGRLIRVNKAWLEMMEYEPNEVLGTVVTKYFTKKSLALFQRKFPAFKE
                     KGVTKNVEFEFITKSGNILICEINGRIEYDKNGKMVVSHCMLNDITFRKQIEKEIREA
                     KQNWQHMFQAISSPTVILNPDFTIVYANDAVLKASKLPIHEIIGQPCCKVFHGKHHEH
                     PPKDCPTKKILEHILSPQVAEMEIQAFNGIFLVSCTPIFTNEGKLDKIIHIATDISEL
                     KKLSTALEESEKRFRELANYLPQTIYECSLDGVFTFVNKSGLEMFGYTQEELEQGLNL
                     MQMLAPSEIQKAKENIAILYKEGKNQGNEYIGKHKNGSTFPILIYTNVVYKNSKPIGI
                     RGILIDLTEHKQTEYIIQENQRVLSSLLANTPGLLYRCKNDLQWTMSFISQQCIAITG
                     YTPDEFIDNKTLSFNDIIAPEYQEIIWNKWQDVINKKSTFTFEYQIIHKSGERRWVYE
                     SGSPIFDEHGALQFLEGYIEDITERKQTELALRHSLLALSEPLDSTENISFTDLFNIT
                     DIQTIQDEFSAATGVSSIITDVHGNPITNPSNFCKLCTKIRDTEKGKELCKRSNADIG
                     KLIQTYSCIETCISVGLPYSGAKIEIGGKHIANWLIGQIRFAETSIEEIIVKTEQLGL
                     QKDETLKSLQSIPIITTERFNEISKLLNSITQELSLKAYQNIQQARFITQIQKNELAL
                     KEKNDEVEAQNEEYQQLNEELAEANKALQKAKETAQRNEEKYRLLFTEMEQGIAVHEV
                     IYNDKNEVVDYRFIDINPAYERITGITKEIALNKRVREILPNVEDYWIETFGKVAVTG
                     IPIHYENTSAELNKHFEVLAYRPQPHQFAVIVTDITERKNAELLLKEKNDEIEAQNEE
                     YLQLNEELAEANRQLEEAKELLQEKEEKYRELVETSQDLIWQCDLEGRFTYVNPGSKQ
                     INGYTPEEMIGKKFTEFLKYDLKERDMEVFNQILEGIDVKGFETIHKHKNGDDVYLIF
                     NARVLTNKLGEVIGTGGTAYDVTDKKRTEKALQLSEDKYKLVVSNTEIISFIIDKQGI
                     FTLSEGKALAKLGLQPGQVVGMSAFEVYKDFPQIISALNRVLQGEVIREELFIADTYF
                     DILYSPIFDNEQNVLLIIGLAVDITERKKTEIILKAKHDEIEAQNEEYMQLNEELVAA
                     NQELYLAKQKAEESDKLKSAFLANLSHEIRTPMNAILGFSDLLADKDLSYEHRSEYVN
                     IVQKSGKHLLSIINDLIDVSKIETGQVSPYYSEISLQTFLKDLYNTLSVTIPEDKTID
                     FSLELHDIDSSSKIISDEVKLRQIIINLVTNALKFTETGFVKIVCSYRDQSQLECKVI
                     DSGVGIAEQYYSVIFDRFRQIEGDLAIKKGGSGLGLAISKAYAEMLGGTITVSSEIGK
                     GSTFTLSIPCYNEYKKPKKHIKKSEHDDNYFGNNETILVAEDDDINFYYLTKIMSKTN
                     YKIIRALNGEEAVTIVKNNPDVKLVLMDIKMPKMNGYEATNIIRSLDANLPVIAQTAY
                     ALSDEQDKIRQTNFTDYISKPLKRDVLLSLIKKHINS"
BASE COUNT         2156 a         1052 c         1094 g         2867 t
ORIGIN      
        1 agaaaggaag actaacatct tccttctctt aattttttat cggacaacag tgatatatat
       61 tattgaaaaa cagataattt gttttgaata tattttactc attttaattt tgtatttaat
      121 gttagaatag ttttttgaat aatcaataat ttttttttgg gtatgttttg agtaattttt
      181 aagtgaattt tcttttaaat tttgatttaa ggggttatta actattgttt gttcgtgtga
      241 attcattttt atatccatta taactgcata gacctttgaa atgagaaata gttgattttg
      301 tataataaaa atttttgatt attagttttt cacaaaatca atacatgtaa aatttaatta
      361 aaaggagata gaggtttttg taatgacatt ttttatatca tcacaaaaaa tattgagtat
      421 gattaagtta aaataattgc agatagttta caatgaaagt attagaaaat ttaaattaaa
      481 ttgtaagttg ttccaaatgg tcgttatata cggtatcaat gtatatgatt ttttgtcttt
      541 taagatatat gtgaacaaaa atatgtctta ttctttattc atgtgaatta tatcttttat
      601 agccagataa attgatgcaa atacagaaag aagtgaacat attatagtac acagtgaatt
      661 gtgtaactca aaataatcat ctaatttttg accgccaaat actccaataa gtataatggc
      721 aagcatttga aaacctatat ttgtgtaggc aagagcgttt ttataattaa gattttttct
      781 caattacttc aatttttttt gcaggatctg caacaaaagt gtcatttttc attgtacatg
      841 taccactaaa aactgctccc ggttctatag caagtttgtt tgttataatg tcaccggtaa
      901 cttttgctgt agatttaaag gaaagtaatt cttgaattgt aattttacct tctaaaactc
      961 cttcaatatc acaattttgg caagaaactt ctccttttat ttcgcctgta ctacctatta
     1021 ctaatttacc ttttgctact aaattacctt taaggcttcc ttctatacga atgtcactat
     1081 tacaagatat atcaccggtt atagtagttc cggcagttat catatttgtt aagaatgatg
     1141 aagtttcgtt aatttttgcc atattattat attttttatt tatacaaata tacaaatgtt
     1201 ccacaaaata cacaaatata tatttgtttt ggtatttttg taaaacaatt gtaatgaaac
     1261 atgatatatc ttactccaac cggttggaaa gactatgaat taatagattc gggtaatttc
     1321 gaaaaattag aaagatttgg tactgttgtt tgtgtccgtc ccgagccaca agctgtatgg
     1381 gataagtcaa tgactgaagc tcaatggaaa caaaaagcaa cagttacatt tcaggcaaaa
     1441 tccagtaata gcggtgaatg gataaaacat gtggcttgtg cagattcatg gaaaattcag
     1501 tatcatccac ttaaacttac gtgtaattta aaatttactt cgttcaaaca tgttggtatt
     1561 tttcctgaac aagcggtaaa ttgggattat atgtatgcaa tttgttcaaa gcatcaaaat
     1621 ttgaatgttt tgaatttgtt tggatatacc ggtatagcat cggttgctgc tcgtgcagca
     1681 ggagcacaag taactcattt agatgctata aagcaagtgg ttaattggac tcgtcagaat
     1741 atggaagaca gtggattgcg cgatattcgt tggataatag aagatgcccg aaaatttgta
     1801 gcacgtgaaa ttaaaagagg caatgtatat caaggtatta taatggatcc tccggcattt
     1861 ggacgaggaa caaatggaga agattggaaa ttggagcgtg atataaatgc tctgcttaaa
     1921 gatgttcaaa agatactaga tacgcaacgc tactttttta tacttaatac gtattcatta
     1981 ggtttttcgg catatataat tgaaaattta ctgcatggaa tttttgctca atattcggta
     2041 cctaaagaag taggagaatt gtatttgcaa gattcttttt caaaaaaatt acctttgggt
     2101 gttattggca gatttactaa tttagtataa ttatttaact gtttatatgt tttttaatta
     2161 aactaagtaa cacatcacgt ttcaaaggtt tgcttatata atcagtaaaa tttgtctgtc
     2221 gtattttatc ttgttcatca ctcaatgcat atgctgtttg agcaataacc ggtaaatttg
     2281 catctaacga acgaataatg ttagttgctt cataaccatt cattttaggc atttttatat
     2341 ccattaaaac taatttaaca tcaggattgt ttttaactat tgtaacggct tcttccccgt
     2401 tgagtgctct gattatttta tagttggttt ttgacattat ttttgtcaaa taataaaaat
     2461 ttatatcgtc gtcttctgca actaaaattg tttcattatt cccaaaataa ttatcgtcgt
     2521 gttctgattt ttttatatgt ttcttaggtt ttttgtattc attgtagcat ggtatagata
     2581 aagtaaatgt agagcctttc ccaatttcag atgacactgt aattgttcca cctaacattt
     2641 ctgcatacgc tttagaaatt gctaatccta atccggaacc accttttttg attgccaaat
     2701 ctccttctat ttgtctgaaa cgatcaaaaa ttacagaata atattgttct gctatgccaa
     2761 cccccgaatc tattacttta cattctaatt gagattggtc acgatatgaa catactattt
     2821 ttacaaatcc tgtttctgta aatttcaagg cgtttgttac taaatttatt ataatttgtc
     2881 ttaattttac ttcatcactt ataattttag aacttgaatc aatatcatgt agttctaatg
     2941 aaaaatcaat agttttatct tcaggtattg ttacgcttaa agtattatat aaatctttta
     3001 aaaatgtttg aagactaatt tcactataat acggcgaaac ttgtccggtt tcaatttttg
     3061 aaacatcaat taaatcatta ataatactca gtaagtgttt tccacttttt tgaacaatat
     3121 ttacatattc gctccggtgt tcgtatgata agtctttgtc tgccaataaa tcggaaaagc
     3181 caagtatagc attcattgga gttcttattt catgagataa atttgctaaa aatgctgatt
     3241 ttagtttgtc actttcttcg gctttttgtt ttgccaaata taattcttga ttggcagcaa
     3301 ctaattcctc atttaattgc atatattctt cattctgagc ttcaatttca tcatgttttg
     3361 cttttagaat tatttcggtt tttttacgtt cggtaatatc aacagctaat cctataatca
     3421 atagcacatt ttgttcatta tcaaatattg gagaatataa tatatcaaaa tatgtgtcgg
     3481 ctataaataa ttcttcgcga attacttctc cttgtaatac tctgttgaga gcagaaatta
     3541 tttgaggaaa atctttatat acttcaaagg cagacatgcc aacgacttgt ccgggttgta
     3601 agcctaattt tgctaaggct tttccttctg ataaagtaaa aataccttgt ttatctatga
     3661 taaaactaat tatttcagtg tttgaaacta ctagtttgta tttgtcttcg cttaattgta
     3721 aagctttttc agtacgtttt ttatctgtta catcataagc cgttccgccg gttccaatta
     3781 cttcgcctaa tttatttgta agtacacgtg cattaaatat taaatatacg tcatcgccgt
     3841 ttttgtgttt atgaatggtt tcaaatcctt taacatcaat accttctaat atttgattga
     3901 atacttccat gtcacgttct ttcaaatcat atttaagaaa ttcggtaaat ttcttaccta
     3961 tcatttcttc gggtgtataa ccgtttattt gttttgaacc aggatttaca taggtaaaac
     4021 gtccttctaa atcgcattgc caaattaaat cttgtgaagt ttctaccaat tcacgatatt
     4081 tttcttcttt ttcttgtaat aattctttag cttcttcaag ttgtctgttt gcttcggcaa
     4141 gttcttcatt taattgaaga tattcttcat tctgagcttc aatttcatca tttttttctt
     4201 tgagtagtaa ttccgcattt tttcgttcag taatatcagt tactattact gcaaattgat
     4261 gaggttgtgg cctataagca agtacttcaa aatgtttgtt gagttctgct gacgtatttt
     4321 catagtgtat gggtataccg gttacagcta ctttaccaaa tgtttcaatc cagtaatctt
     4381 ctacatttgg taaaatctca cgaactcgtt tgtttaatgc tatttctttt gttattccag
     4441 taatgcgttc atacgcagga tttatatcaa taaatctata atcaacaact tcgtttttgt
     4501 cgttatatat aacttcatgc acagcaattc cttgctccat ttcagtaaac aacaatcggt
     4561 atttttcttc atttctttgt gctgtttctt tagctttttg taaagcttta tttgcctctg
     4621 caagttcttc gtttaactgt tgatattctt cgttttgtgc ttctacttca tcattttttt
     4681 ctttgagagc aagttcattt ttttgaattt gtgtaataaa ccgtgcttgt tgaatatttt
     4741 gatatgcttt aagggataat tcttgtgtta tactgttaag taatttggaa atttcattaa
     4801 atcgttcggt ggtaattatt ggaatagatt gtaatgattt tagtgtttca tctttttgta
     4861 aacctaattg ttcagttttt acaataattt cttcgatgga agtttcagca aaacgaattt
     4921 ggccaatgag ccaattagca atatgttttc cacctatttc aatttttgct ccggaatatg
     4981 gtaatcctac cgaaatacaa gtttcaatac aagaatatgt ttgtataagt ttcccaatgt
     5041 cagcattaga tcgtttgcat aattctttac ctttttctgt atccctaatt tttgtgcata
     5101 gtttgcagaa gttagaagga ttagttatag gattgccgtg cacatcggta attatagaag
     5161 aaacaccggt tgctgcagaa aattcatctt gtattgtttg tatatctgtt atgttaaata
     5221 aatcggtaaa agaaatattt tcggttgaat ctaatggctc agataatgct aataatgaat
     5281 gacggagagc taattctgtt tgttttcgtt cggtaatatc ttctatatat ccctctaaaa
     5341 attgcaatgc accatgttca tcaaatattg gactgcccga ttcatatacc caacgacgtt
     5401 cacccgattt atggattatt tgatattcaa acgtaaatgt agatttttta tttataacat
     5461 cttgccattt attccatatt atttcttggt attcgggtgc tattatgtca ttaaatgata
     5521 gagttttatt atcgataaat tcgtcaggag tatatcctgt aattgcaata cattgttggc
     5581 ttataaatga cattgtccat tgtaaatcgt ttttacaacg gtacaaaagt ccgggggtat
     5641 ttgccagtaa tgacgataaa acacgttggt tttcttgtat tatatattct gtttgtttat
     5701 gttcggttag gtctattaaa atacctcgta taccaatggg tttagaattt ttgtatacta
     5761 cattagtata aattaatata ggaaaagttg agccgttttt atgtttacca atatattcgt
     5821 taccttgatt tttgccttct ttatagagaa ttgcaatatt ttctttggcc ttttgaattt
     5881 cacttggagc aagcatttgc atgaggttta atccttgttc aagttcttct tgagtatagc
     5941 caaacatttc gagaccgctt ttgtttacaa aggtaaatac accatctaat gagcattcat
     6001 atatagtttg tggtaaataa ttagctaatt ctctgaatcg tttttcgctt tcttctaaag
     6061 cagttgataa ttttttgagt tcacttatat cggtagctat atgaattatt ttatctaatt
     6121 tgccttcgtt ggtaaatata ggcgtgcatg atactaaaaa aatgccgttg aatgcctgaa
     6181 tttccatttc ggcaacttgt ggtgagagta tatgttctaa tatttttttt gtagggcagt
     6241 ctttaggcgg gtgttcatga tgtttgccgt gaaagacttt gcaacacggt tgtcctatta
     6301 tttcatgtat aggcaatttg cttgctttta gtacggcatc gttggcatat actatggtaa
     6361 aatcaggatt taaaattact gttgggctcg atattgcttg aaacatgtgt tgccaatttt
     6421 gtttagcttc acgtatttct ttttctattt gttttctgaa agtaatatca tttaacatgc
     6481 aatggcttac aaccattttg ccgtttttat catattctat tctgccattt atttcacata
     6541 ttagtatgtt gccgcttttt gttataaatt caaattctac attttttgta actcccttct
     6601 ctttaaaagc cggaaacttc cgttgaaata atgctaatga ttttttggta aaatattttg
     6661 ttactactgt tcctaatact tcgtttggtt catactccat catttctaac catgctttat
     6721 ttacacgaat tagtcgacca tattcatcaa gtgaatgata tgctaaaggc atatcttcaa
     6781 ttaatgtttc aaaataattg ttagaataag tattccgcat tgacagaata tgttgttgta
     6841 atatttcaat ccgtttgtgt aattctatac attcatctga ttgatattgg tgagaatctg
     6901 tcatagcaaa atattatgat gagtatttaa aactattttt tatacacgaa tgtacgaatt
     6961 tttgttacaa taaatgtgta tttttttgtg atttttatca attattataa ttttatcata
     7021 tgtattgtgt agggtaaata ttattacgat tgtatttatt acgaaattta atactattat
     7081 aaaatttaaa aaattataga acgatgagaa accatctatt tcttttatta tttgcattat
     7141 gttcacagat tgcattttct caaattaat
//
LOCUS       DAOTOW010000013         7122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9183_length_7122_cov_4.170228, whole genome shotgun sequence.
ACCESSION   DAOTOW010000013 DAOTOW010000000
VERSION     DAOTOW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_7122_cov_4.170228"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="P5243_00480"
     CDS             <1..986
                     /locus_tag="P5243_00480"
                     /inference="COORDINATES: protein motif:HMM:NF005744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS17950.1"
                     /translation="KKLAEYYKQANIDISYEDILITTGGSEAICFAYYSILNYADEVI
                     VPEPFYSNYYGFTTETGARMIPVRSTIETSFALPNIEEFEKALTPRTKAIMLCNPNNP
                     TGYVYSKNELEQIRDLVKKYDLFLMCDEVYREFCYDSPHYSVMNLKGIEENTILIDSF
                     SKRYNVCGIRIGALISRNKEVIATSLKFAQARLSPPSFGQIAAEALLDTPKEYLLASA
                     QEYKERRNYIINELNSIKGVRSPMPKGAFYSIVELPIDDAEKFCQWLLEEFEFEGSTV
                     MFAPGNGFYTGKKLGENQVRIAYVLEHDKLEKAMRCLREALRVYPGRTIDQ"
     gene            988..1866
                     /locus_tag="P5243_00485"
     CDS             988..1866
                     /locus_tag="P5243_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRS17951.1"
                     /translation="MQTKVLFIINPNSGTKRKKNIEELAKQYLPDSEFICYFIYTKHR
                     GHATEICKDAVLNFDIIVAVGGDGTIHEIAQHIIHTKAALAIVPMGSGNGLARFLRIP
                     LSQKKAIQLIAKKQIQTIDTILCNNEYFINVAGFGFDARIGDVFDKTPNRGGVKYIKL
                     VGKELFNYKPQTLSFLSEDTSFSKTFFIASVANSSQWGMNAHIAPMADIRDGYFDVVF
                     IKKFPMRAALRLVWKLFSKQIHTSKYVETFKTHSLTVTNNTSCVLHLDGEPRTFTGDI
                     TFTMQKRSLHMVVGDI"
     gene            complement(1902..1975)
                     /locus_tag="P5243_00490"
     tRNA            complement(1902..1975)
                     /locus_tag="P5243_00490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1939..1941),aa:Arg,seq:acg)
     gene            complement(2074..4122)
                     /locus_tag="P5243_00495"
     CDS             complement(2074..4122)
                     /locus_tag="P5243_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRS17952.1"
                     /translation="MKLHIIHIVTFISISSFAFATHIPIILLNDSTHYSLQKHSVGFF
                     EDTSTTLTIYDVISPQTDIDFTPMDKVKENRGFSSSAFWLKMEFKNDGNHTHYYHLTI
                     EYPLLNDVRFFKVQDSIIKDVVITGENFPYKSRQIQNRFFIFKIEIPINSQITYYIRV
                     FNVGEATRLPLSLTHIDETANIQARSDFFSSIYYGYILFSIIFLVFMYLEFKRIHFLF
                     LALYTFSMAFFLFIVDGYAFQFLWPNFTYISNISIIFFALISAVSLLYFTNIFLESHT
                     IVKNITQVLSAIAIIIFIWNLFPYPYNYFSFISVNIFSMIVVCFIAILAIYKYYKNRN
                     HYNTLFLFSLIFILLTVLIYVFRNFAIFDIPIISANSIKLGLAGQITLLSVSTITQFR
                     EFFQKSNKFLEEMIQKRTVLISSQNNQLQEQNKKIAQQYKEIKQSMTYAKRLQNAILP
                     NKYKFSSVFKDHLILYKPRDIVSGDFYWIAEKRKKTYIVTADCTGHGIPGAFLSMLGI
                     SFLNQIVSKNHDISPHDILHKLGLLFSETINNHEDNMTRDSMDISICLIDHTTKIMEY
                     AGAYNPIYIIRNQELYELTVDKFSLHKDYASEMPKFTNKIYTLQENDRVFMFTDGYAD
                     QFGGENNKRYSKRRFKQLLVQSASKPMSEQKTILEMTHDNWKGKLEQVDDILVLGFEI
                     "
     gene            complement(4225..4785)
                     /gene="rimM"
                     /locus_tag="P5243_00500"
     CDS             complement(4225..4785)
                     /gene="rimM"
                     /locus_tag="P5243_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRS17953.1"
                     /translation="MITKDTFFLIMAYSFPSISDCVPIGKISRPHGNEGFVLISLHNE
                     YQSICKEIEYVFLLIQNKLVPFFIQKCTVKGGATYMQFDDITSIDLAEKICGNTLYIP
                     SQFIKEDDDTLDELHQLIGFKIFSNTEYIGIIKDVFEYSMNIVLEIETQTNDEKLIPF
                     STNLVIDLNYENQTLIMNLPEGIFDI"
     gene            complement(4817..5374)
                     /locus_tag="P5243_00505"
     CDS             complement(4817..5374)
                     /locus_tag="P5243_00505"
                     /inference="COORDINATES: protein motif:HMM:NF011094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRS17954.1"
                     /translation="MAVKIRLARHGRKASPFYHIVAADSRAPRDGKFIEKIGTYNPTS
                     HPTKVELKFDRALYWLNNGAEPTDTARSILKNEGVLYMKHLQGGVKKGAFDEATLEKK
                     FQAFLEEKRKKEEALISKLSSDKQAALKKRAAAELEVSKKREEAILKKQQAAEASAEE
                     TVTEETTEESTEATDNTAAEETPAE"
     gene            5568..>7122
                     /locus_tag="P5243_00510"
     CDS             5568..>7122
                     /locus_tag="P5243_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17955.1"
                     /translation="MSIQKKILTAIILLCLIGIISFIYIFLKKKTQVIQNTVYEVLSD
                     NTVAFVELKSLSDFFELQKSNHAIWDDVSPFPLFNETVQFAKILDSVISKNIEFTSFL
                     QNNFVASMQQVGKHKLEYVFCIPLNSEQQTTLAPKILQSAFSQYIINSYNYDGKCTIY
                     NFLTPGSNVNKISCTICNNIIIASASRIHVENAVRSFYDGTNIMKNPQFAKVKKTAGS
                     KVYGNVYINFNLFSKVLSLVMRNDLKQQFTEKYHFASWSEFDITPKENALLLNGFTTV
                     NNPDEYLNIFKKQQPVKLSITSMIPAGIAGFVSIGISNKDAFRENYEAFLRKTDIYNP
                     YKQGISEINAAFSSPNQDAVNCIKNFNAFIADEIALVYGNVGETDLYENSFVICKTVG
                     QQQTLDVLLPMLDSYCEKEKKSISDITTSYSIDNTTNYKIYSLPISTIPKVLWGNLFS
                     QVSAKYITCIQDYIVFGPSIQALQRYISAFELQKTIANELEFKKIQNNILSTYSLYVY
                     SNIAKSNKLY"
BASE COUNT         2450 a         1002 c         1145 g         2525 t
ORIGIN      
        1 gaaaaaaact ggcagaatat tataaacaag ctaatataga tatttcatac gaagatattt
       61 taataactac cggtggttcc gaagcaattt gttttgctta ttatagtatc cttaattatg
      121 ccgatgaggt aatcgttcct gaaccttttt attctaatta ttatggtttt acaaccgaaa
      181 caggtgctcg gatgattccc gttcgttcta ctattgaaac aagttttgca ttgccaaata
      241 ttgaagagtt tgaaaaggct ttaactcctc gaacaaaagc tatcatgctt tgtaatccta
      301 ataatccaac agggtatgtg tattcaaaaa atgaattgga acaaatacga gatttggtta
      361 aaaaatatga tttgtttttg atgtgtgatg aagtgtatag agaattctgt tatgatagtc
      421 cacattattc tgtaatgaat cttaaaggaa tagaagaaaa tacaattctt atagattcat
      481 tttcaaaacg ttacaatgta tgcggaattc gaataggtgc tcttatatca cgaaataaag
      541 aggttatagc aacttcactt aaatttgctc aagcacgctt gagtcctcca agttttgggc
      601 aaattgctgc cgaagcttta cttgataccc caaaagaata tttattggct tcggcacaag
      661 aatataaaga acgtagaaat tatattataa acgaattaaa ttctataaaa ggggtacggt
      721 ctcctatgcc taaaggtgct ttttattcaa ttgtagaatt gccaatagat gatgctgaga
      781 aattttgtca gtggttactt gaagagtttg aattcgaagg ctctacggta atgtttgctc
      841 cgggtaacgg attttacacc ggtaaaaaat tgggagaaaa tcaggtacga attgcctatg
      901 tattggaaca cgataaatta gaaaaagcaa tgcgttgttt acgcgaagca ttgcgagtat
      961 accccggaag aactatagac caataatatg caaacaaaag ttctttttat aataaatcca
     1021 aattcgggta ctaaacgcaa gaaaaatatt gaagagcttg caaagcagta tttgcctgat
     1081 tctgaattta tttgttactt tatttataca aaacaccgtg gacacgctac agaaatttgc
     1141 aaagacgccg ttttaaattt tgatattatt gtagctgtag gaggcgatgg aacaattcat
     1201 gaaatagcac aacatattat acacaccaag gcagcacttg ccattgttcc catgggctcg
     1261 ggaaatggac tcgctcgttt tttgagaatt ccattatcac agaaaaaagc aattcagtta
     1321 attgcaaaaa aacagataca aacaatagat actattttgt gtaataatga atattttata
     1381 aatgttgcag gttttggttt tgatgctcgt attggtgatg tatttgataa aacaccaaat
     1441 cgtggtggag taaaatatat aaaattagtt ggtaaagagt tatttaatta taaacctcaa
     1501 acattatctt ttctttcaga agatacctca tttagtaaaa cattttttat tgcatcggtt
     1561 gctaattcaa gccagtgggg aatgaatgct catattgctc ctatggctga tattcgtgat
     1621 ggatattttg atgtggtttt tattaaaaaa tttccaatga gagctgcttt acggttggtt
     1681 tggaaattat tttcaaagca aattcataca tcaaaatatg ttgaaacatt taaaacacat
     1741 tcgcttactg taacaaataa tacatcatgc gtattgcatc tcgacggtga accaagaaca
     1801 tttaccggag atattacctt tacaatgcaa aagcgttcgt tacatatggt tgtaggtgat
     1861 atataaaaaa aagcgaattg cttataaaac aactcgcttt gtgactccgg agagactcga
     1921 acccccaacc ttctgatccg tagtcagatg ctctatccaa ttaagctacg gagccatttt
     1981 tttgaggtgc aaatatactt atttttttat catcacaaac attagcatga aaaaagtgaa
     2041 taaaaaaaca ggtaagtatt gaattaacgg taattagatt tcaaatccta aaacaagaat
     2101 atcatcaact tgttctaatt ttcctttcca attatcatga gtcatttcta gtattgtttt
     2161 ttgttctgac ataggttttg atgccgattg aacaagcagt tgtttaaatc gacgctttga
     2221 atagcgtttg ttgttttcgc ctccaaattg atctgcatac ccatcggtaa acataaacac
     2281 acgatcgttt tcttgtaatg tatatatttt attggtaaat ttaggcattt cagacgcgta
     2341 atctttatgg agtgaaaatt tatcaaccgt aagttcatat aattcttgat tgcgtatgat
     2401 ataaatagga ttatatgctc cggcatattc cattattttt gttgtatgat caattaaaca
     2461 aatagaaata tccatactgt cacgagtcat attatcttca tgattattga tagtttccga
     2521 aaataataat cctaatttgt gtaatatgtc atgaggagaa atatcatgat tttttgatac
     2581 tatttgattt aaaaatgaaa tacccaacat actcaaaaat gctcccggaa tgccatgacc
     2641 ggtgcaatcg gctgtaacta tatatgtttt ttttcgtttt tcggcaatcc aataaaaatc
     2701 gccgcttact atatcgcgtg gtttatataa aatcaagtgg tctttaaata ccgacgaaaa
     2761 tttatattta tttggtaaaa ttgcattttg tagccgtttg gcatacgtca tactttgttt
     2821 tatttctttg tattgttgtg ctattttttt attttgttct tgtaattggt tgttttgtga
     2881 actaattaat acagtacgtt tttgaatcat ttcttccaaa aatttatttg atttttggaa
     2941 aaattcgcga aattgagtta ttgttgaaac cgataaaagc gttatttgtc ctgcaagtcc
     3001 taatttaatg ctgtttgctg aaatgattgg aatatcaaat atcgcaaaat tcctaaaaac
     3061 atatataagt accgtaagta aaataaatat aagcgaaaac aaaaagagag tattgtaatg
     3121 atttcggttt ttataatatt tatagattgc taaaattgca ataaagcaaa ctacaatcat
     3181 gctaaaaata ttcacgctta taaaactgaa atagttataa ggatatggaa ataagttcca
     3241 tataaaaata ataatagcta tagcagaaag tacttgtgtt atgtttttta caatagtgtg
     3301 tgattcaaga aaaatattgg taaagtataa taaagataca gcagatatta aagcaaagaa
     3361 aataatagaa atgtttgaaa tgtatgtaaa atttggccac aaaaactgaa aagcatatcc
     3421 atcaactata aaaagaaaga atgccatact aaaggtgtat aatgctaaaa atagaaaatg
     3481 tattctctta aattctaagt acatgaatac tagaaagata atagaaaata aaatgtaccc
     3541 atagtatatt gaagagaaaa aatcggaacg ggcttgaatg tttgcagttt catctatatg
     3601 agtgagtgac agggggagtc gagtagcttc acctacatta aatactctaa tgtaataggt
     3661 tatttgagaa ttaattggta tttctatttt gaatataaaa aagcgattct gaatttgacg
     3721 gcttttgtac gggaaatttt caccggtaat taccacatct tttataattg aatcttgaac
     3781 cttaaaaaaa cgtacatcat tcaaaagagg atattcaata gtaagatgat aataatgagt
     3841 atggttacca tcgtttttaa attccatttt aagccaaaat gccgatgatg aaaaacctct
     3901 attttctttt actttatcca tgggagtaaa atcaatatcg gtttgtggtg aaattacatc
     3961 gtatattgta agtgtggtag atgtgtcctc aaaaaaacca acactatgct tttgcaatga
     4021 atagtgtgtt gaatcattaa gaagaattat tggtatatga gtggcaaatg caaatgaaga
     4081 aatactgata aatgtaacta tatgtattat atgtaatttc aaacccctat tttttttaat
     4141 tcttatgctt gtacgaatag aattttaaat aggtttgtat caaatagtgt tttttttgaa
     4201 caaactcaat gatttatatc attgttaaat atcaaaaata ccttcgggta aattcattat
     4261 aagagtttga ttttcatagt tcaaatcaat aactaaattg gtagaaaatg gtattaattt
     4321 ttcatcgtta gtttgagtct caatttcgag aacaatattc atagaatatt caaaaacgtc
     4381 ttttattatg ccaatatatt cagtatttga aaatattttg aatccaatta gttgatgtaa
     4441 ttcatctaaa gtgtcatcat cttctttaat gaattgagac ggtatataca gtgtgtttcc
     4501 acatattttt tcggctaaat ctattgaggt aatatcgtca aattgcatat atgtagcacc
     4561 tccttttact gtacattttt gaataaaaaa aggaactaat ttattttgaa taagtaaaaa
     4621 aacatattca atttctttgc aaatagattg atattcattg tgtaatgaaa tcaaaacaaa
     4681 cccttcgttt ccatgtggtc tggaaatttt cccaataggg acacagtctg atattgaggg
     4741 gaatgagtat gccataatca aaaaaaaggt gtcttttgta atcaaaagac accttgtgtt
     4801 attttctgaa tattatttat tctgcaggag tttcttctgc agctgtattg tcagtagctt
     4861 cagttgattc ttctgtagtt tcttcagtta cagtttcttc ggcacttgct tcagcagctt
     4921 gttgtttttt aagaatagct tcttcacgtt ttttgctcac ttctaattct gcagctgcac
     4981 gttttttgag agcagcttgt ttatcagaag ataattttga aataagagcc tcttcttttt
     5041 tgcgtttttc ttctaaaaat gcttgaaatt ttttttcaag tgtagcttcg tcaaaagcac
     5101 cttttttaac accgccttgt aagtgtttca tatacaatac accttcgttt ttcaatatag
     5161 aacgtgcagt atctgtaggt tctgctccat tatttaacca ataaagagct ctgtcaaatt
     5221 ttaattctac tttagttggg tgcgaagtag gattatatgt accgattttt tcgataaatt
     5281 taccatcacg tggtgctctg ctatctgcag ctacaatgtg atagaaagga ctcgccttcc
     5341 gtccgtgtct tgctaatctg atttttacag ccattgtatt tgtatatgtt taaaaattaa
     5401 taaaaattag gctgcaaaaa tacatctttt ttttacgaaa caacgagttt tgtttatttt
     5461 ttaaaaatat gtatttctat actttttttt atttcgaaaa aatacactgc tcagtactat
     5521 tatttcaaga gtttttatac atttgtaata gttcaaaaac tattctcatg agtattcaaa
     5581 agaaaatatt aacagctata attcttcttt gtttaattgg tataatttct tttatatata
     5641 tatttttaaa aaagaaaaca caagtaattc aaaatactgt atatgaagtg ttgtctgata
     5701 atacagttgc ttttgttgaa ttaaaatcac tttctgattt ttttgaatta caaaaatcca
     5761 atcatgctat atgggacgat gtttctcctt ttcctttgtt taatgaaacg gttcaatttg
     5821 caaaaatact tgattcggtt atttcaaaaa atattgaatt tacctcgttt ttgcaaaata
     5881 attttgtggc ttccatgcag caagttggta aacacaaatt ggaatatgtg ttttgtattc
     5941 ctctaaattc tgaacaacaa accacgttag ctccaaaaat attgcaatca gcattttctc
     6001 aatatataat aaattcatat aattatgatg ggaaatgcac tatatataat tttcttacac
     6061 cgggttcaaa tgttaataaa atttcatgta ccatatgtaa taatattatt atagcttcag
     6121 cttcgcgtat tcatgttgaa aatgccgtca gaagttttta cgatggaact aatataatga
     6181 aaaacccgca atttgcaaag gttaaaaaaa ctgccggaag caaagtgtat gggaatgtat
     6241 atataaactt taatctgttt tctaaagtac tttctttagt aatgcgtaat gatttaaaac
     6301 agcaatttac agaaaaatat cattttgcat cgtggagtga atttgatata acacctaaag
     6361 aaaatgcgtt attactgaat ggatttacaa ctgtaaataa tccagatgaa tatttgaata
     6421 tatttaaaaa acaacaacct gtaaaattaa gtattacatc tatgattcct gcgggcattg
     6481 cagggtttgt gtctattggt atttctaata aagatgcttt tcgcgaaaat tatgaagcat
     6541 ttcttagaaa aacagatatt tataatcctt ataagcaggg aattagcgaa ataaatgctg
     6601 cgttttcttc accaaatcaa gatgcggtaa attgtataaa gaattttaat gcttttatag
     6661 cagatgaaat tgctttagta tatggtaatg tgggcgaaac cgatttgtat gaaaattctt
     6721 ttgtgatatg taaaactgta ggacaacagc aaacattaga tgtgttgttg cctatgcttg
     6781 acagctattg cgaaaaagaa aaaaaatcta tttcagatat taccacttca tattctattg
     6841 ataatactac aaattataaa atatatagtt tgcctatatc tacaattcct aaggtattgt
     6901 ggggtaattt attttcacaa gtttcggcaa aatatattac atgtattcag gattatatag
     6961 tgtttggacc aagtattcaa gctttgcaac gatatataag tgcttttgag ttgcaaaaaa
     7021 ctattgcaaa tgaacttgaa tttaagaaaa ttcagaataa tatattgtca acatattctt
     7081 tgtatgtcta ttcaaatatt gcaaaatcaa ataaattata tg
//
LOCUS       DAOTOW010000014         6951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9454_length_6951_cov_3.957077, whole genome shotgun sequence.
ACCESSION   DAOTOW010000014 DAOTOW010000000
VERSION     DAOTOW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9454_length_6951_cov_3.957077"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1883)
                     /gene="hcp"
                     /locus_tag="P5243_00515"
                     /gene_synonym="priS"
     CDS             complement(219..1883)
                     /gene="hcp"
                     /locus_tag="P5243_00515"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRS17956.1"
                     /translation="MSMFCFQCQEAAKGTGCTIKGVCGKTEEVSNLQDLLIYVSKGVS
                     WYAHSAHTNNIAVDQADTFVFEALFMTITNANFDDEKIIAKIKQAMQVRTAVVDVLQQ
                     NNVSIPTEIPSQAKIMSDTDEKITAQSLLVSILNETNEDIRSLKELITYGLKGMAAYA
                     EHAHNLGKRSTNMYGFMHRAMAATIRIDISADELIALTLETGKYGVDAMALLDAANTE
                     AYGNPEITKVNIGVRNNPAILISGHDLKDMEELLKQTEGTGVDVYTHSEMLPAHYYPA
                     FKKYSHFVGNYGNAWWKQKEEFESFNGPVLFTTNCIVPPKASYIDRIYTTGATGVNGC
                     THIPDRTDGQPKDFSAIIEHAKRLASPTEIETGEIIGGFAHNQVLQLADKVVDAVKSG
                     AIRKFVVMAGCDGRMNSRDYYTEFAKQLPHDVVILTAGCAKYRYNKLALGDINGIPRM
                     LDAGQCNDSYSLAVIALKLKEVFELNDINELPIAYNIAWYEQKAVIVLLALLYLGVKN
                     IHLGPTLPAFLSPNVANVLVENFGIGGIGTVDEDIKQLLGESVESL"
     gene            complement(1974..2873)
                     /locus_tag="P5243_00520"
     CDS             complement(1974..2873)
                     /locus_tag="P5243_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS17957.1"
                     /translation="MIREIVTIDEDLCNGCGECVPNCHEGALQMIDGKARLISDLMCD
                     GLGACIGHCPVGAITLEKREAEAYNETKVMEIMVSKGKNTVLAHLKHLKDHGEIDFLR
                     EGVRYLKSHESTIDFDVSEVIFTVHNYGKPAAHQVQGGHAMHHAGGGCPGSQTIAFDV
                     PKLQIESIAVSGQSQLRQWPVQLHLVNPMAQYFQQADVLLAADCVAFSLGNFHDTYLK
                     GKGLAIACPKLDHGSDIYIQKLSAMIDQAKINTLTVMIMEVPCCGGLVQMARQAVSQA
                     SRKIPIKAIIVSIKGEILSEEWI"
     gene            2977..3687
                     /locus_tag="P5243_00525"
     CDS             2977..3687
                     /locus_tag="P5243_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRS17958.1"
                     /translation="MLEPCSNNCSFCFVQYTDVMSTENLFRGIDRQEIGEIIRNVHHQ
                     VRNYKTGEVLIYEEDEYNSLLILVQGAVRTEMMNPEGQLLVLEQLDAPATLAPGALFA
                     SQPHIPVSVVAQSDCRALIISRESMNQILATNPQVLTNFVRILSNRVQFLSNRLKSLQ
                     FQSLRSKFAQHVLQQYVLQKSTQIVLKKTQQELSEMFGVARPSLARVVRDMHNEGIIH
                     AKGKHIEIRNIDMLRKTK"
     gene            3847..6834
                     /gene="secDF"
                     /locus_tag="P5243_00530"
     CDS             3847..6834
                     /gene="secDF"
                     /locus_tag="P5243_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRS17959.1"
                     /translation="MQNKGFIRVFAILLVLAALYSLSFTYYTWKASRDADAYAHGDAQ
                     KRKEFLDSLTQVDDYYNFLGLRKFSYSDCQQHELNLGLDLKGGMNVTLQISIKDMIMN
                     LSNEGEDDTVLMKTLQRADVLERSSKEEYVTLFGKAFAELYPNKSLRTLFIANEDMQS
                     KITFNSTNEEVLAVLSSEAKAAIDNSFNIIRTRIDRFGVTQPNIQNLGGGRILVELPG
                     VQDQDRVRDLLQSSAKLEFWETYNNSEIYSVIFEINKTLAALNALKENKDTVKKAKEV
                     SKADTTKKTELSLKEQLKQDTTKLAKADSTKSKEQIQKENPLFSRFIPNISQDGRPGI
                     GPVVGFAQKSDLAAIDSILRMPEVREIIDANAPELKLTWSKKSNDNGFYSLVALKDLD
                     RDGKASLEGDVVVDARPEFSQMGGSASVRMVMNSDGAKEWRRLTKENIQRSVAIVLDG
                     YVYSFPTVQTEIADGISSITGNFTIPEATDLANILKSGKLSAAAEIINAEFVGPSLGK
                     ESINKGIISFLVAFVIVLLYMFLYYQKAGLVANIALLLNLFLIFGVLASLGAVLTLPG
                     IAGIVLTLGMAVDANVIIYERIREEMAQGKGMKLAIADGFKNAYSAIIDANVTTIIIA
                     IILYLFGKGPIQGFATTLIIGVLTSLFCGIFITRLIFERMLTKKRNITFDTKLTRNAF
                     KNVNFDYIGTRKIYYIISSVIILIGIVSMFARGWNVGVDFKGGRSYIVQIDDNKNTAP
                     IEKSLQVQFGKAPEVKTFGASEIKITTDYMINEKGPTVDSLVEARLFEGLKPILPQGT
                     DFDGFMKNHLRQSDMVGPTIANDIIWSAIISVVLALLFIFLYIFVRFRNWQFGMGAVA
                     ALFHDVLVVLGIFSLLHGIVPFSLEIGQSFIAAILTVIGYSINDTVIVFDRVREITIE
                     FPKRQTIKLFNQGVNSTISRTINTSLTTILVLLIIFLFGGDSIKGFVFAMLIGIVIGT
                     YSSLFVASPITFDLLKKQNKIIE"
BASE COUNT         2223 a         1212 c         1231 g         2285 t
ORIGIN      
        1 cactaagact caaaggttca ttctactttt tatctacttt attgtatttt ttagcgtctt
       61 ggcgacttcg tagctttttt tatagtgcca caaagacaca aagactcaaa ggttcattct
      121 actttttatc tactttattg tattttttag catcttggtg acttcgtggc ttttttatag
      181 tgccacaaag acaccaacat acactaataa tattaagatt acaatgattc aaccgattct
      241 cctagtaatt gtttaatatc ttcgtcaact gttccaatgc cgccaatacc aaaattttca
      301 acaagtacgt ttgccacatt tggcgataaa aatgccggta aggttggacc taaatgtatg
      361 tttttaactc ctaaatataa taatgcaagg agcacaatta cagccttttg ttcgtaccaa
      421 gcaatattgt atgcaattgg caattcgtta atatcgttga gttcaaatac ttctttcagt
      481 tttagtgcaa ttactgcaag tgaatacgaa tcgttacatt gtcccgcatc aagcatacga
      541 ggtatcccgt taatatcgcc cagagctaat ttgttgtacc gatattttgc acagcctgct
      601 gttaatatta ccacatcgtg cggtagttgt ttcgcgaatt cggtatagta atcgcgagaa
      661 ttcatacgac catcgcagcc tgccataaca acaaatttac gaatagcccc cgattttaca
      721 gcgtctacta ctttatcggc aagttgcaaa acttggttgt gagcaaatcc tccaattatt
      781 tcacctgttt ctatttcggt aggcgatgcc aatctttttg catgttctat aattgccgaa
      841 aaatcttttg gttgtccgtc ggttcggtcg ggaatatgtg tgcacccgtt aacaccggta
      901 gctcctgttg tataaattct atcgatatac gacgctttgg gaggaacaat acaatttgtg
      961 gtaaacaata ccggaccatt gaaactttcg aattcttctt tttgcttcca ccacgcgtta
     1021 ccgtaatttc caacaaagtg cgaatatttt ttgaatgcag ggtagtagtg tgccggcaac
     1081 atttcgctat gggtatacac gtctactccg gttccttcgg tttgtttgag caattcttcc
     1141 atatctttga ggtcgtgacc acttataaga attgccggat tgttgcgaac cccaatattt
     1201 actttggtaa tttcgggatt tccatatgct tcggtatttg cagcatcgag caatgccata
     1261 gcatctacac catatttgcc ggtttccaga gtaagggcaa ttaattcatc agccgaaatg
     1321 tcgatacgga tagtagcagc catagcacgg tgcataaatc catacatgtt ggtacttcgt
     1381 tttcctaaat tgtgagcgtg ttcggcatac gctgccatac ctttaagtcc ataggttata
     1441 agctctttga gcgaacggat atcttcgttg gtttcgttca aaatacttac caataaactt
     1501 tgtgcagtta ttttctcgtc ggtatccgac attatttttg cttgactcgg aatttctgtt
     1561 ggaatactta cattattttg ttgcaagaca tctacaaccg cagttcgcac ttgcatagct
     1621 tgtttaattt ttgcaataat tttttcgtca tcaaaattgg cattggttat agtcataaac
     1681 aaagcttcga acacaaacgt atcggcttgg tcaaccgcta tattatttgt gtgtgcacta
     1741 tgagcgtacc acgatacccc ttttgataca tatataagta aatcttgcag attcgaaact
     1801 tcttcggttt ttccacatac tcctttgatg gtacatccgg tacctttagc tgcctcttgg
     1861 cattgaaaac aaaacattga catttttttt ataattttta attcttaatt tttaattttt
     1921 aattgaagaa agattttcta attcataatt tttaattcat aattaaaaat tatctatatc
     1981 cattcttccg atagtatttc tcccttaata cttactatta ttgctttaat tggtatttta
     2041 cgacttgctt gcgaaactgc ttgacgagcc atttgaacta gtccaccgca gcatggcact
     2101 tccattatca ttacggtgag tgtgtttatt tttgcttggt ctatcatggc acttaatttt
     2161 tgaatataga tatcgctgcc atggtctaat ttaggacacg ctatagccaa acctttgcct
     2221 ttgagatagg tgtcgtgaaa atttccgagc gaaaaagcta cacaatctgc tgccagaagt
     2281 acgtcggctt gttggaaata ttgagccata ggatttacca aatgtaactg taccggccac
     2341 tgacgcaatt gcgattgtcc gcttacggca atactttcta tctgaagttt tggaacatca
     2401 aacgcaatag tttgactacc cgggcaacca ccgccggcat gatgcatagc atggccacct
     2461 tgcacttgat gtgcagcagg tttaccataa ttatgcacag taaatattac ttcgcttaca
     2521 tcaaaatcta tagtagattc atgtgatttt aagtagcgta ccccttcgcg taagaaatca
     2581 atttcgccat gatcttttaa atgtttgagg tgcgctaata cggtattttt acctttcgaa
     2641 accataatct ccataacttt ggtttcgtta tatgcttctg cttcgcgttt ttctaaagtt
     2701 atagcaccca ccgggcaatg tccaatgcat gcaccaaggc catcgcacat aaggtcgcta
     2761 atcaatcgcg ccttgccatc aatcatttgc aacgcccctt catgacagtt tggaacacat
     2821 tcgccacagc cattgcaaag gtcctcgtct atcgttacta tttctcgtat catgtgaata
     2881 atattttttt aagtatagtg caaacatata gcgataaaat atgtaatttt gtaacatgtg
     2941 ttacaaaata tatattttta tcaaaaaatg aaaaacatgc ttgaaccttg ttctaataat
     3001 tgtagttttt gttttgttca gtataccgat gtaatgagta ccgagaattt atttcgtggc
     3061 attgacagac aagaaatagg agaaattatt cgcaatgtac atcatcaagt tcgaaattat
     3121 aagactggag aagtgcttat atatgaagag gatgaatata attccttgct tatactggtg
     3181 caaggagcgg tgcgaacaga gatgatgaat cccgaaggtc agttgcttgt tttggaacaa
     3241 ttagatgcac cggccacatt agcacccggg gcattatttg cgtcgcagcc gcacataccg
     3301 gtaagtgtag tagcgcaaag cgactgtaga gctcttatta tttcgcgcga atctatgaat
     3361 caaatattgg caactaatcc tcaagtactt accaattttg ttcgcatatt atcaaataga
     3421 gtacagtttt tatcaaatcg tttaaaatcg ttgcagtttc aaagtttacg gtcaaaattt
     3481 gcacaacatg ttttacagca atatgtttta caaaaaagca ctcaaatagt attaaaaaag
     3541 acacaacaag aattgtcgga aatgtttgga gtagcacgcc catcgcttgc cagagtagtt
     3601 cgggatatgc acaacgaagg tatcatacat gcaaaaggta aacatattga aatacgtaat
     3661 atagacatgc tacgtaaaac taaatagcaa aaactcttac tagttgctca gaaaatacct
     3721 taaaaataaa ataataataa aaaccccata tatattaaaa aaataatata tttgcgggta
     3781 aaaattttga aggttataaa aaacctgaag taacttattt taaactaatt ataaaaattt
     3841 cacacaatgc agaacaaagg ttttattcgt gtatttgcaa tattattggt attagcagct
     3901 ctatattcat tatcatttac gtattatact tggaaagcaa gtagagatgc tgatgcatat
     3961 gcacacggcg atgcacaaaa acgtaaagaa tttcttgatt ctctaacgca agttgatgat
     4021 tattacaact ttttagggtt acgaaaattt agttattccg attgtcaaca acatgaatta
     4081 aatcttggtc ttgaccttaa aggaggtatg aacgttacgc ttcaaatttc gataaaagat
     4141 atgataatga atttatcgaa cgaaggcgaa gatgacactg ttttaatgaa aactttacaa
     4201 cgtgccgatg tattagaacg ttcttcaaaa gaagaatatg taacattatt tggaaaagca
     4261 tttgcagaat tatatcctaa taagagtttg cgtactttat ttattgcaaa tgaagatatg
     4321 caatcaaaaa tcacatttaa ttctacaaat gaagaagttc ttgctgtgtt aagttccgaa
     4381 gcaaaagccg caatagataa ttcattcaat atcataagaa cacgtattga ccgttttggg
     4441 gtaacacaac caaacattca aaatcttggt ggaggacgca tattagttga attaccggga
     4501 gtacaagatc aagaccgtgt gcgtgattta ttacaaagtt ctgcaaaatt agaattctgg
     4561 gaaacttaca acaattcaga aatatactcg gttatatttg aaataaataa gacattagca
     4621 gctcttaatg cattaaaaga aaataaagat acagtaaaaa aagcgaaaga agtttcaaaa
     4681 gctgacacaa ctaaaaaaac agaattatca cttaaagaac agcttaaaca agacactaca
     4741 aaacttgcaa aagctgacag caccaaatca aaagaacaaa ttcaaaaaga aaatccatta
     4801 ttctcacgat ttattcctaa catatcacaa gatggtcgtc ctggtatagg ccctgtagta
     4861 ggttttgcac aaaaatctga tttggcagca atagattcta tacttagaat gcccgaagta
     4921 cgtgaaataa ttgatgcaaa tgcaccggaa ttaaaactta catggtctaa aaaatcaaac
     4981 gacaatggtt tttattcatt agtagccctt aaagatttag atcgtgacgg caaagcgtca
     5041 ttagaaggtg acgtagttgt agatgctcgt cctgaattta gtcaaatggg aggttctgca
     5101 agtgtacgaa tggtaatgaa cagtgatgga gctaaagaat ggagacgatt aacaaaagaa
     5161 aacattcaac gttcggtagc tatagtactt gacggatatg tttattcatt cccgacagta
     5221 caaacagaaa ttgccgatgg aatatcatct ataacaggta attttactat accggaagct
     5281 actgacttag caaatatttt aaaatcagga aaactttcgg cagctgcaga gattataaat
     5341 gctgaatttg taggcccttc acttggtaaa gagtcaatta ataaaggtat catttcattt
     5401 ttggtagctt ttgttatagt tcttttatac atgtttttat attatcaaaa agcagggctt
     5461 gttgcaaata ttgcattatt gcttaactta tttttaattt ttggtgttct tgcatcgctt
     5521 ggcgctgtat taacattgcc cggtattgca ggtatcgtgc ttacgttggg tatggcggta
     5581 gatgccaatg tgataattta tgaacgtatc cgtgaagaaa tggctcaagg taaaggtatg
     5641 aaattagcaa ttgcagacgg ttttaaaaat gcatattcgg caattataga tgctaacgtt
     5701 acaactataa ttatagcaat aattttatac ttattcggta aaggacctat acaaggtttt
     5761 gctactactc ttatcattgg agtgttaaca tcattattct gcggaatctt tattactcgt
     5821 ttaatatttg aaagaatgtt aactaaaaag agaaacatta cttttgacac aaaactaact
     5881 cgtaatgcat ttaaaaatgt gaattttgat tatataggaa ctcgcaaaat atattatata
     5941 atatcatcgg ttattatact tataggaatt gtatcaatgt ttgcacgcgg ttggaatgtt
     6001 ggtgttgact ttaaaggtgg acgttcatat atagtacaaa ttgatgataa taaaaatacc
     6061 gcacctattg aaaaatcatt gcaagtacag tttggaaaag ctccggaagt aaaaacattt
     6121 ggcgcgagtg aaataaaaat tactacagat tacatgatta atgaaaaagg tcctacagta
     6181 gacagtttag ttgaagcacg tttatttgaa ggattaaaac caattttacc acaaggaacc
     6241 gattttgatg gatttatgaa aaaccattta cgtcaatcag atatggttgg acctactatt
     6301 gcaaacgaca ttatatggag tgctatcatt tcagtagtat tagctctatt gtttattttc
     6361 ttatacatat ttgttcgttt cagaaactgg caatttggta tgggagcagt tgcagcatta
     6421 ttccacgacg tgttggttgt attgggtata ttctctcttc ttcacggtat tgttccattc
     6481 tcattagaaa taggtcaatc atttatagca gcaattctta ccgtgattgg ttactctatc
     6541 aacgatacag tgattgtgtt tgaccgtgta cgtgaaataa ctatagaatt ccctaaacgt
     6601 caaactatta aattatttaa tcaaggggta aatagtacta taagtcgtac aatcaatact
     6661 tcgttaacta caatattagt attattaatc atattcttat ttggtggtga ttctatcaaa
     6721 ggatttgtgt ttgcaatgtt aataggtatt gttataggaa catattcttc attatttgtt
     6781 gcgtctccaa ttacatttga tttgttgaaa aaacaaaata aaattattga ataatacgat
     6841 gtattatact atacaaaaaa ggctgcttca gaaatgaggc agcctttttt gattgtatgt
     6901 gtaagcttcc catttttagg acagtgttta attataatgt aacaaagata t
//
LOCUS       DAOTOW010000015         6926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9501_length_6926_cov_4.005385, whole genome shotgun sequence.
ACCESSION   DAOTOW010000015 DAOTOW010000000
VERSION     DAOTOW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9501_length_6926_cov_4.005385"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="P5243_00535"
     CDS             <1..294
                     /locus_tag="P5243_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17960.1"
                     /translation="EYGTVPVVEKNVYPLVPAVPYYTSDYDLSAFTNWKYGVGFRYSP
                     LYGLARANIPYSEKKLLKFKYVDMRYVRYNRSDGLIAWSTTIDCGFVIETKKE"
     gene            368..1204
                     /locus_tag="P5243_00540"
     CDS             368..1204
                     /locus_tag="P5243_00540"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HRS17961.1"
                     /translation="MAQKTLFIITSILLCIAACVNGITNTTNSDFFETNVTTLLYIPW
                     FETKYLYCIIHAIVIIPIIALSFDKKVYFARTYTSILPAILITGLLFIMWDFAKTHIH
                     VWGFNSKYLLNIFIWNLPIEELLFFITIPTAFMFIYVCFEIYALMPRHNVPDIITPTL
                     IIGLIIIGIYFWQYTYTSTVTLFAASTLCLHLAIGNAYIRLKTYLVFIVSLIPFFFIN
                     GILTGAFQNEPIVWYNTEELLNIRIISIPIEDIIYLLPLLLINTSLFEWFRQKFSQKS
                     PI"
     gene            1262..2422
                     /locus_tag="P5243_00545"
     CDS             1262..2422
                     /locus_tag="P5243_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRS17962.1"
                     /translation="MNNFEYYNPVNIVFGKQTIAKLSQLIPTNAKVMLLYGGGSIKHN
                     GVYDQVIAALKGFSYIEFGGIEPNPRYETMMKALELAKKENVTFLLPVGGGSVIDGTK
                     FLAAAMFVPEGIDPWGILQGTIKVTKALPIGAVLTLPATGTEMNGNSVVSRNSTNEKL
                     AFGSQLVMPQFSILDPETCFSLPDKQIANGIVDAFIHVMEQYLTIPNNTPLQDRWAES
                     ILTTLIEVAPIVMKNKTNYDAMATFMWTATMALNGIISRGCIEDWSTHIIGHELTALY
                     DIDHGRTLAIVLPGNMNIRRQVKGDKIIQYGQRVWNITQGSRDEKINKTIEKTVEFFE
                     SLGVPTTLKAYTIGADVCKTVSQRLASRGLTKFGEAGDITPEVVEAILKDRL"
     gene            2432..2977
                     /locus_tag="P5243_00550"
     CDS             2432..2977
                     /locus_tag="P5243_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRS17963.1"
                     /translation="MQNNQILIQSFANECTMRTMYDMFAKQARSEGYIRIAQMFDEFA
                     LHELSHAKNFQKFFDNSIVDVSMHMTIPKLDTTIENIRTAIAAEIHDAELYGTYSEIA
                     KAEGNTKLSAKFKHIAIAEQFHRKQLEKVLYELKNNLYFYSNEPIEWYCIKCGYTHVG
                     ETPPLECPACNHPQGYFTQTL"
     gene            3009..3581
                     /locus_tag="P5243_00555"
     CDS             3009..3581
                     /locus_tag="P5243_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRS17964.1"
                     /translation="MSIKGTETEKNLLKSFAGESQARSRYTFFAKQAKKEGYEQIANI
                     FMETAEQEKEHAKRFFSYLEGGDVEITAMYPAGKVGTTAENLEAAAMGENEEWTLLYP
                     QFAEVAEKEGFKEIATLFKMIAKVEVEHEKRYLRLLENVKNDKVFEKDEATRWKCTNC
                     GYVHDGKKAPEKCPACQHPKAYFQVKEENF"
     gene            3800..5518
                     /gene="lysS"
                     /locus_tag="P5243_00560"
     CDS             3800..5518
                     /gene="lysS"
                     /locus_tag="P5243_00560"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRS17965.1"
                     /translation="MQQLSEQELIRRDSLQKLRELGIEPYPAQEFKVTAYSQEILDKF
                     TPESTEFTNVTLAGRIMSRRIMGKASFCELQDSYGRIQLYINRDEICPGEDKTLYNDV
                     FKRLLDIGDFIGVTGYVFITQVGEISVHVSSFIVLSKSLRPLPIVKEKDGVVYDAFTD
                     PEQRYRQRYVDLVVNPEVKQVFLQRTKIINTMRNFFNEKNYVEVETPILQPIPGGASA
                     RPFITHHNALNIPLYLRIANELYLKRLIVGGFEGVYEFAKDFRNEGMDRTHNPEFTVM
                     EIYVAYKDYNWMMDFTEEMIERVALALHGKTQVQVGDNIIDFKRPFKRITMYDAIKEH
                     TGVDISNMNEAELRATCDKLSIHHEPSMGKGKLIDEIFGETCEASYIQPTFIIDYPVE
                     MSPLCKKHRNNPNLTERFELMVNGKELCNAYSELNDPIDQYERFKEQLELSAKGDDEA
                     MFIDHDFVRALEYGMPPTSGMGIGIDRLCMFMTNSPSIQDVLFFPQMKPEIKSHKDDI
                     EVYTSAGIPQEWAEVLQTMGYVKYAALTEIKYTKVHQELCGYNKKHKLDLVNPKPEDV
                     KLWLQQ"
     gene            5523..6518
                     /locus_tag="P5243_00565"
     CDS             5523..6518
                     /locus_tag="P5243_00565"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRS17966.1"
                     /translation="MNNSTKIALLGSGSWATAIAKILHTSLDSLVWYIREPEIIEGIR
                     KHKSNPLYVSDIKFATEKLHITNNVNEAIAQADIILFCVPSKFIISLCDNITEPLSHK
                     HIVTAIKGIEPGSNKIISTYLQDRFNVPQEQVAVISGPCHAEEVALERLSYLTIGSPN
                     YDFAAHIAKIMNVWFIKTCVSSDIVGIEYAAVLKNIMALATGISHGLGYGDNFTSVLI
                     SNAIQEMKRFLDTVEPTNRDINDSVYLGDLLVTAYSQFSRNRTFGMMIGKGYSVKSAE
                     LEMNMIAEGYYGVNCIHAINEQYNVDMPITQAVYHILYDRISPSMEFKILAESFK"
     gene            6636..>6926
                     /locus_tag="P5243_00570"
     CDS             6636..>6926
                     /locus_tag="P5243_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRS17967.1"
                     /translation="MANSLFIPKNYQSPLNLKQTEQAIKLIKDTFEHNLTAELRLRRV
                     TAPLFVLKGTGINDDLNGTERKVTFPIKGLNDQQAEVVNSLAKWKRLALAEYE"
BASE COUNT         2462 a         1198 c         1222 g         2044 t
ORIGIN      
        1 gaatatggca cggtgcctgt agttgaaaaa aatgtgtatc cgcttgtacc tgctgttcct
       61 tattacacgt ccgattatga cttatcggca tttaccaatt ggaaatatgg cgtaggattt
      121 cgctactcgc ctctctacgg attggcacga gcaaacattc catactccga aaaaaaatta
      181 ttaaaattca aatatgttga catgcgatac gtacgctaca atagaagcga cggacttatt
      241 gcatggtcca caaccataga ttgtggtttt gtaattgaaa caaagaagga gtaatttaac
      301 cttaaactca cgtaaattta tatgtaaaaa ttctctatgt ttgcatacat caatcaagat
      361 gcaagatatg gcacaaaaaa cgctattcat aattacttca atactattat gcatagcagc
      421 atgtgtaaat ggtataacta ataccacaaa ctcagatttt tttgaaacaa acgttactac
      481 tctattgtat attccatggt ttgaaaccaa gtatttatat tgtattattc acgctattgt
      541 aattataccc atcattgctt tatcattcga caaaaaagta tattttgcac gcacatatac
      601 aagtatttta cctgccattc ttattaccgg acttctattt ataatgtggg attttgctaa
      661 aacacacatt cacgtatggg gatttaattc aaagtattta ctcaatattt ttatttggaa
      721 tctcccaatt gaagaattat tgttttttat tacaataccc acagcattta tgtttattta
      781 tgtatgtttt gaaatatatg cgttaatgcc tcgccataat gtacctgata taattacccc
      841 tacattaata attggattga taattatagg aatttatttt tggcaataca cctatacttc
      901 tactgttact ttatttgcag cctctactct atgtttacat cttgctattg gcaatgcata
      961 tattcgttta aaaacatatc ttgtctttat agtatcactc attccatttt tttttataaa
     1021 tggaatctta accggagcat ttcaaaatga acccatagtt tggtacaata ctgaagaatt
     1081 attgaatata cgtataattt caatccctat agaagacatt atttatttac tccctctttt
     1141 acttataaac acatcattat ttgaatggtt tcgccaaaaa tttagtcaaa aatcaccgat
     1201 ataatacata agcgtactat caaattatag tatatttgat aaattttaaa tatgtataat
     1261 tatgaacaat tttgaatatt ataatccggt aaacattgta tttggtaaac aaacaatagc
     1321 aaaactttca cagttaatac ctacaaatgc aaaagtaatg ttgttgtatg gcggtggaag
     1381 tattaaacac aatggtgtat acgaccaagt aatagcagca ctcaaaggtt tttcgtatat
     1441 agaatttggc ggtattgagc ccaacccacg gtacgaaact atgatgaagg ctttggaatt
     1501 ggcaaaaaaa gaaaatgtta catttttatt acctgtaggt ggtggttcgg taatagatgg
     1561 aaccaagttt ttggcagcgg ctatgtttgt tcccgaaggt atcgacccat ggggaatatt
     1621 gcaaggaact attaaagtta ctaaagcttt accaataggc gcagtattga ccttacctgc
     1681 aaccggaact gaaatgaacg gaaattcagt ggtttcgcgc aatagcacca acgaaaaact
     1741 tgcatttggc agccaattgg ttatgccgca attctctata ctcgaccccg aaacatgttt
     1801 ttcgttgccc gacaaacaaa ttgcaaacgg tattgtagat gcatttatac atgtaatgga
     1861 acaatattta acaattccaa ataacacccc actgcaagac cgctgggcag aaagcatact
     1921 tacaacactt atagaagtgg ctcctatagt tatgaaaaat aaaaccaact acgatgctat
     1981 ggctacattc atgtggacag caaccatggc attaaatgga atcatttcaa gaggctgcat
     2041 tgaagattgg agtacacaca taattggaca cgagcttacc gcgctgtatg atatagacca
     2101 tgggcgcaca ttagcaatag tgttgcccgg caatatgaat attcgccgtc aggtaaaagg
     2161 cgataaaatc atacaatatg gccaacgtgt atggaatata actcaaggct cgcgcgatga
     2221 aaaaatcaac aaaacaatag aaaaaacagt agaatttttt gaatcgttgg gagttccaac
     2281 tacgcttaag gcatatacta ttggtgcaga tgtgtgcaaa acagtgtcgc aacgattagc
     2341 atcgcgagga cttaccaaat ttggtgaagc gggcgatatt acacccgaag tagttgaggc
     2401 tattttgaag gatagattat aaaattgagt tatgcaaaat aatcaaattc tcatacaatc
     2461 attcgcaaac gagtgcacta tgcgtactat gtacgacatg tttgcaaagc aagcacgtag
     2521 cgagggctat attcgtattg ctcagatgtt cgatgaattt gccctacacg aattgtcgca
     2581 tgccaaaaat tttcagaaat tctttgacaa ttcgattgtt gatgtttcta tgcatatgac
     2641 tatccctaag ttggatacaa cgattgaaaa tatccgtact gctatagctg ccgaaataca
     2701 tgatgctgaa ctctatggta cgtatagtga aattgcaaaa gcagaaggaa acacaaaact
     2761 ttcggcaaaa ttcaaacata ttgcaattgc cgaacaattt catagaaaac aattagaaaa
     2821 agtattgtat gaattgaaga ataatttgta tttttacagc aatgagccta tagaatggta
     2881 ttgtataaaa tgtggatata cacacgttgg cgaaactcca ccgctggaat gtccggcatg
     2941 caatcatccg caaggctact ttacacaaac gttgtaacat attatttatt aactataaat
     3001 cacagattat gagcataaaa ggaactgaaa cagaaaaaaa tttattaaaa tcgtttgccg
     3061 gtgaatcgca agcacgtagc cgctatacgt tttttgcaaa acaagctaaa aaagaaggct
     3121 atgagcaaat agcaaacatt tttatggaaa cagccgaaca agaaaaagaa catgcaaaac
     3181 gcttttttag ttatttagaa ggtggcgatg tagaaattac tgcgatgtat cctgccggaa
     3241 aagtaggtac cactgccgaa aatttggaag ctgctgcaat gggcgaaaac gaagaatgga
     3301 cactcttata tccacaattt gccgaagtag cagaaaaaga aggttttaaa gaaattgcaa
     3361 ctttatttaa aatgatagca aaagttgaag tagaacacga aaaacgatac ttacgtttac
     3421 tcgaaaatgt aaaaaacgac aaagtattcg aaaaagacga agctactcgt tggaaatgta
     3481 ccaattgcgg atatgttcac gatggcaaaa aagcacccga aaaatgccct gcatgtcaac
     3541 atccaaaagc ttacttccaa gtaaaagaag aaaattttta attgaattat taaaacatac
     3601 aagaaagctt tttgcatatg agcaaaaagc ttttttttgt aacaacagaa ccattttaag
     3661 gtttaagttg aaaatggttc tgttttaatt tctttccatt attttaaata tttcgctttg
     3721 cgaaattgcc tcgcttattg tttgattaat tgctataaat agtatatttg cctaaaattt
     3781 taacaataaa aggacatata tgcaacaatt gagcgaacag gaattgattc gaagagattc
     3841 cttgcaaaaa ttacgcgaat taggtataga gccttacccg gcacaagaat ttaaagttac
     3901 tgcctattca caagaaatct tagacaaatt tactcccgaa agtactgaat ttaccaatgt
     3961 taccttggct ggtagaatca tgtcgcgacg tattatgggc aaagcgtcat tttgtgaatt
     4021 acaagattca tacggacgca ttcaattata cataaatcgc gacgaaattt gtccgggcga
     4081 agacaaaaca ttgtataacg atgttttcaa acgtttgctc gatattggag attttatagg
     4141 tgtaaccgga tatgttttta taacacaagt tggtgaaatt tctgtacatg taagcagttt
     4201 tattgtatta agcaaatcac tcagacctct cccaattgta aaagaaaaag acggtgtagt
     4261 atacgatgca tttaccgacc ccgaacaacg gtatcgccag cgatatgttg acttggtggt
     4321 aaatcccgaa gtaaaacaag tatttttaca acgcaccaaa ataataaata caatgcgtaa
     4381 tttttttaac gaaaaaaatt atgtagaagt agaaactcct atattacaac ctattcccgg
     4441 tggagcttcg gctcgtccgt ttattacgca ccacaatgca ctcaatatac cactatattt
     4501 gcgtatagca aacgaattat acctaaaacg actcatagtt ggcggttttg aaggtgtgta
     4561 tgaatttgct aaagacttcc gcaacgaagg tatggaccgc acacacaatc cggaatttac
     4621 cgtaatggaa atatacgtag cttacaaaga ctacaattgg atgatggact ttaccgaaga
     4681 aatgatagaa cgtgtggctc ttgcattaca cggaaaaaca caagtacaag ttggcgataa
     4741 cataattgac tttaaacgtc cgtttaaacg aattacaatg tacgatgcta ttaaagagca
     4801 taccggtgta gatataagta atatgaatga agctgaactt cgtgctacgt gcgacaaact
     4861 tagcattcat cacgaaccaa gtatgggtaa aggtaaattg attgacgaaa tttttggtga
     4921 aacatgcgaa gcatcataca ttcaaccaac atttattata gactatccgg ttgaaatgtc
     4981 gcctctgtgt aaaaagcata gaaacaatcc aaaccttacc gaacgatttg aacttatggt
     5041 aaacggtaaa gaattgtgta atgcatattc agaattgaat gatcctattg accaatacga
     5101 acgtttcaaa gaacaattgg aactttcggc aaaaggtgac gatgaagcaa tgtttattga
     5161 tcatgacttt gtgcgagcat tggaatatgg tatgcctcct acttcgggta tgggtatagg
     5221 aatagaccgt ttgtgtatgt ttatgaccaa ttcgccttca attcaagatg tattgttttt
     5281 cccgcaaatg aaacccgaaa taaaatcaca taaagatgac atagaagtat atacatcggc
     5341 aggtattcca caagaatggg cagaagtatt acaaactatg ggctatgtaa aatatgcagc
     5401 attaaccgag attaaatata ccaaagttca tcaagaacta tgcgggtaca acaaaaaaca
     5461 caaacttgat ttagtaaatc ctaaacccga agatgtaaaa ctgtggttac aacaataagc
     5521 ctatgaataa ttcaacaaaa atagcattgt taggcagtgg cagttgggct accgctattg
     5581 caaaaatact ccatacatca ttagattcac ttgtatggta tatacgcgaa cccgaaatta
     5641 ttgaaggtat tcgtaaacat aaatcaaatc cattgtatgt atcagatata aagtttgcaa
     5701 cagaaaaact tcacattacc aacaacgtaa acgaagccat tgcacaagct gatataatac
     5761 tattctgtgt cccttcaaaa tttatcatat cattgtgtga taatataaca gaaccattat
     5821 ctcacaaaca tattgtaaca gctataaaag gcatagaacc cggaagcaat aaaataatat
     5881 caacatattt acaagataga ttcaacgtac cccaagaaca ggtagcagta atttccggtc
     5941 cgtgtcatgc agaggaagtt gcacttgaac ggttatcata tcttactatt gggtcaccca
     6001 attacgattt tgcagctcac attgccaaaa ttatgaatgt gtggtttatt aaaacctgcg
     6061 tttcgagcga tattgttggg atagaatatg ccgccgttct taaaaatatt atggcattgg
     6121 ctaccggtat aagtcacgga ttgggatacg gcgacaattt tacttctgta ctcatttcca
     6181 atgccattca agaaatgaaa cgatttttag atactgtaga gccaaccaat agagacataa
     6241 acgattcggt atatttagga gatttattag ttacggcata ttcacaattc agtagaaatc
     6301 gaacctttgg tatgatgata ggcaaaggat attcggtaaa aagtgccgaa ttagaaatga
     6361 atatgatagc cgaaggatat tatggtgtaa attgcataca tgctattaat gaacaataca
     6421 atgtagatat gcctattact caggctgtat atcatattct ttacgataga atttctccat
     6481 ctatggaatt taaaatttta gcagaaagtt ttaaataatc catcaatttc ataaaaatta
     6541 ctgttgttta ttatacattg atagtaaatc aaagtttttt ttataatttt gtaataattt
     6601 gtaacaaatt actatagaaa caataataca atataatggc aaatagtcta tttataccta
     6661 aaaattatca atcgccgctc aatctgaagc aaactgagca agcgataaaa ttaataaaag
     6721 acacgtttga gcataattta accgccgaat tacgactacg aagagtaacc gctcccctat
     6781 ttgtattgaa aggtaccggt ataaatgacg acctcaacgg aaccgaacgt aaagttacat
     6841 tccccataaa aggattaaac gaccaacaag ccgaggtagt aaactcattg gcaaaatgga
     6901 aacgattagc tttggcagaa tatgaa
//
LOCUS       DAOTOW010000016         6828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9657_length_6828_cov_3.640189, whole genome shotgun sequence.
ACCESSION   DAOTOW010000016 DAOTOW010000000
VERSION     DAOTOW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_6828_cov_3.640189"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /gene="gap"
                     /locus_tag="P5243_00575"
     CDS             complement(<1..989)
                     /gene="gap"
                     /locus_tag="P5243_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRS17968.1"
                     /translation="MAIKIGINGFGRIGRFVFRQAIAKGTVEVVAINDLIDVDYMAYM
                     LKYDSTHGQFKGTVEVKDGKLIVNGKAIRVTAEKNPADINWGAVGADYVVESTGLFLE
                     KSKAQAHIDGGAKKVIMSAPSKDDTPMFVMGVNHKSYTKDMNFVSNASCTTNCLAPIA
                     KVLNDNWGIVEGLMTTVHATTATQKTVDGPSAKDWRGGRGAGQNIIPSSTGAAKAVGV
                     VIPELKGKLTGMAFRVPTPDVSVVDLTCRLAKPAKYSEICAAMKAASEGELKGILGYT
                     EDEVVSNDFIGDTRTSIFDAKAGIQLSDTFVKVVSWYDNEMGYSAKVCELIEY"
     gene            complement(1174..1710)
                     /locus_tag="P5243_00580"
     CDS             complement(1174..1710)
                     /locus_tag="P5243_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS17969.1"
                     /translation="MLADFHPHAICKYCPKCGSKGFKPHSPKSQKCDVCGFVFFTNAA
                     AAVAAIITNTQGEILLTTRAFDPGKGLLDLPGGFVDPGESIEQALHREIYEELNLHIS
                     SSTYIGSFPNEYVYGGILYYTIDLLFECDIKDISTIRVADDVSAYGFYKPTQELIEQV
                     GLPSIKKILAHYTMSDTK"
     gene            complement(1719..1988)
                     /locus_tag="P5243_00585"
     CDS             complement(1719..1988)
                     /locus_tag="P5243_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17970.1"
                     /translation="MANKRTFKKEITLFLELVFEECLFTREFNPSKSVQIDELIDQAV
                     SLYTLTISARKTEANLTYKEFYTALQEVYYSKILEILNSLEELRK"
     gene            complement(2000..3307)
                     /locus_tag="P5243_00590"
     CDS             complement(2000..3307)
                     /locus_tag="P5243_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS17971.1"
                     /translation="MNIPISKQIIDTALHRNGIHDIAKASIRQIVKLVNELETETGVE
                     FVKMEMGVPGLSPASIGVDAEIQALKNGVASIYPNIEGIPELKTEMARFVQSFLNVTV
                     SPQSCVPTVGSMQGAFACFLTACRSNAAKTKLLFIDPGFPVQKQQLQILGLAFESFDV
                     YNYRGAALRQKLEEYCSSGTISTILYSSPNNPTWICFTDEELQIIADIASKYDIIVIE
                     DLAYFGMDFRTDYGIPGQPPYQPTVAQYTKNYILLISASKAFSYAGQRIAALIVSDEL
                     AKRTYPDLLRYFSSAEFGKALIFGALYGLSSGTAHSVQYAFAALLREANNGTYNFVKD
                     VQEYGRRAKAMKELFVKYGFSIVYDTDIDKPIADGFYFTISYKHLKGSELVYELLQYG
                     ISAITLDITGSERTQGLRACTSQIGNKQLNYLQERLPLFNTNH"
     gene            complement(3317..4987)
                     /locus_tag="P5243_00595"
     CDS             complement(3317..4987)
                     /locus_tag="P5243_00595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRS17972.1"
                     /translation="MNICKHICFFVTVLVAVSCSSQKTARVGKAETVDYTYPKFTQKD
                     SLRGALNPFRTCYNVLHYTLTIDVNPQKKSIQGYVDMQFSVMQHTEKMQVDLFKNMTM
                     DSIVLHGKRLKFERKYDAVFIQIPQLEIGKTETIRMYYKGKPLEAPRPPWEGGFVWKK
                     DEQKNPWIGVACELVGASLWWPCKDHLSDEPEYGVTMNVTVHQGLTVVSNGKLLEQKS
                     YKGKDTFTWQTKYTINNYNVTLYIGKFEHFSEKYKGIDTTITLDYYVLPEHLEKTKIT
                     FQQTSNILRYYESIYGPYPWPTEGFKLVGSPYEGMEHQTAIAYGTGFRTHHLGFDYII
                     LHESAHEWWGNSISVDDFADIFIHEGFATYSEALFIEHTYGVKEAERYMYKYAGYIKN
                     KNPIVGPRDVNFWDYKDTDPYMKGAWTLHGLRYVINNDSLFFDILKTYYNTRAYSIVT
                     VKDFTDFVQQKSGMNLEWYFTQYLYSRHVPTLEYVAYKKDNIVTIEMRWANVQSNFAL
                     PVQIDLNGEPIRVLPSTEPKRFVFHNVTEIELNPYKAYFIVKKVESFK"
     gene            complement(4999..5571)
                     /locus_tag="P5243_00600"
     CDS             complement(4999..5571)
                     /locus_tag="P5243_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17973.1"
                     /translation="MSKQVIGFIVVCLVSIHASFAQYNFFTAQTNDSLTTDFYKRSLR
                     VTTIVLYQMNVHDIGASFFIHNSLDRISYYIECKTNLPGRYVITGNQLQGLGVSQMIV
                     PYRSFSCNIGIARAVTRNWFVYAAPGFVAQRTYYTNTENSLFTYTIPKHGMWYTATFG
                     GMYAANNKLSAQAGIDIFNKNLLVGIGYTF"
     gene            complement(5603..>6828)
                     /locus_tag="P5243_00605"
     CDS             complement(5603..>6828)
                     /locus_tag="P5243_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HRS17974.1"
                     /translation="LFMLKSDFVEHSQAIPQAQVRFVLTAEISSIYKKNGKTRKVHTI
                     LWAPNFDIVTKVQHAFIQRNLNIASDGRPIIGMDARDLAALCKDISPDIHIVPAHIWT
                     PWFSALGAKSGFDSIAECYGDMEPYIFAVETGLSSDAPMNWMCSFLDRYALLSNSDAH
                     SPDKLGRNANVFDCDISYNAIVHALQHKELKQFVGTIDTYPQAGKYHFSGHRKCGVCA
                     TPVEVLQHAFVCKTCKKPYTFGVMDRVMQLADRRDISMCYNWQSIEYIISLPELLAQA
                     YNVSETSKKVKAIYMQMIQHLGAEFEILTRCEISDIAQCSNSFVAEGIRRMRAGEVYV
                     TEGYDGEYGDIRVFTNDEVSEFVKNTEYIPSKNIHKPLQRFYFDLHAYDKEAQKTDFS
                     LLHGKKPNGQLQLFL"
BASE COUNT         2211 a         1270 c         1007 g         2340 t
ORIGIN      
        1 atatactcta tcaattcaca tacttttgca gagtaaccca tttcattgtc ataccatgat
       61 actactttta caaatgtatc tgataattga atacctgctt tagcatcgaa aatagaagta
      121 cgagtatcgc caataaagtc gttagaaaca acttcatctt cagtatatcc aagaattcct
      181 tttaattcac cttctgaagc agctttcata gcagcacaaa tttcagaata ttttgcaggt
      241 tttgctaaac gacaagttaa gtcaactaca gaaacgtctg gagttggaac acggaatgcc
      301 atacctgtta atttaccttt aagttcagga attaccacac ctaccgcttt tgcagcacct
      361 gtagatgaag gaataatatt ttgtcctgca ccacgaccac ctctccaatc tttagcagat
      421 ggaccgtcaa cagttttttg agttgcagtt gtagcgtgta ctgtagtcat aagaccttct
      481 acaatacccc aattatcgtt aagaacttta gcaataggag ctaaacagtt tgttgtacaa
      541 gatgcgtttg atacaaaatt catatctttt gtatatgatt tgtgattaac acccattaca
      601 aacattggag tatcatcttt tgatggagct gacatgatta cttttttagc accaccatct
      661 atatgagctt gagcttttga tttttctaag aataaacctg tagattctac aacataatca
      721 gcacctacag caccccagtt aatatcagca ggattttttt cggcagttac acggatagct
      781 ttaccgttta caattaattt accgtcttta acttcaacag ttcctttgaa ttgaccgtga
      841 gttgaatcat attttaacat gtatgccatg taatcaacat caattaagtc gttaattgct
      901 acaacttcaa cagttccttt tgcaatagct tgacggaaaa caaaacgtcc gatacgtcca
      961 aatccgttaa taccaatttt aatagccata ataataaata tttataagat aattaatgaa
     1021 acaatatttt taattggtac aaatttatgt aaaaaatata atatacaaaa tcatttgaaa
     1081 ggtgattcat ttttttttac taaaatgtat cagtattttt tgtgtgtgaa cgcatatttt
     1141 tgattttcaa tacaaacact aatactattt tttctatttt gtatcactca tagtatagtg
     1201 tgcaagaatt tttttgattg aaggtaatcc aacttgttct atgagttctt gtgttggttt
     1261 ataaaaacca tatgcagaaa catcatcggc cactcgtatg gttgatatat ccttaatatc
     1321 acattcaaac aataaatcaa tggtataata tagaattcct ccatatacat attcattagg
     1381 aaacgaacct atatatgtac tcgatgaaat atgtaaattg agctcttcat aaatttcgcg
     1441 atgaagtgct tgttcaatag attctcccgg atcaacaaaa cctccgggta aatcaagtaa
     1501 acctttgccg ggatcaaaag cccgagtggt taataatatc tcaccttgtg tattggtaat
     1561 aattgcagct actgctgctg ctgcatttgt aaaaaataca aacccacaca catcacactt
     1621 ttgtgatttt ggagaatgag gtttaaatcc tttactaccg catttagggc aatatttaca
     1681 tattgcatga ggatgaaaat ctgcaagcat actgcttatt attttcgcaa ttcttctaat
     1741 gaattaagta tttcaagtat ttttgaatag tatacttctt gtaaagctgt ataaaattct
     1801 ttatatgtaa gattcgcttc tgttttgcga gccgaaattg ttaacgtata taaacttacc
     1861 gcttgatcta tcaattcatc tatctgtaca gatttagagg gattaaattc tcttgtaaat
     1921 aaacattctt caaacactaa ttctaaaaat agtgttatct cttttttaaa tgtgcgtttg
     1981 tttgccataa tataagtatt taatgatttg tattgaagag aggtaatcgt tcttgcaaat
     2041 aattcaattg tttatttcct atttgcgatg tgcaagcacg taaaccttgt gtgcgttcac
     2101 ttccggtaat atctaatgta attgcactga ttccatattg caataattcg taaaccaatt
     2161 cactcccctt taaatgttta tatgaaatgg taaaataaaa tccatctgct ataggtttgt
     2221 caatatcggt atcatatact attgaaaatc catactttac aaataattct ttcatagctt
     2281 tggctcttcg tccatattct tgcacatctt ttacaaaatt atatgttcca ttgtttgctt
     2341 cacgcaacaa tgccgcaaat gcatattgta ccgaatgtgc agtgcccgat gataagccat
     2401 acaatgctcc aaatataaga gctttgccaa attctgctga tgaaaaatat cgtaacaaat
     2461 caggatatgt tcgtttagct aactcatcag aaactataag agctgctatt cgttgacctg
     2521 cataactaaa tgcttttgat gccgaaatca ataatatata atttttggta tattgcgcta
     2581 ctgtaggttg atatggaggt tgtccgggaa tcccataatc tgttctaaaa tccataccaa
     2641 aataagctaa gtcttctatt acaattatat catacttact ggcaatatca gctattattt
     2701 gcaattcttc atcagtaaaa catatccacg taggattgtt tggtgacgaa tacaaaatag
     2761 ttgatatagt accactgctg caatattctt ctaatttttg tcgtaatgca gcaccacgat
     2821 aattatatac atcaaatgat tcaaatgcta atcctaaaat ctgtaattgc tgtttttgta
     2881 ctggaaaacc cggatcaata aacaataatt tcgtttttgc cgcattgctt ctacaagccg
     2941 ttaaaaaaca ggcaaatgct ccttgcattg acccaacagt tggcacacat gattggggtg
     3001 atacagtaac atttaagaaa gactgaacaa agcgagccat ttctgttttt agttcaggaa
     3061 taccttcaat attaggataa atagaagcta caccattttt taatgcttgt atttctgcat
     3121 ctactcctat tgacgccggt gacaatccag gcacccccat ctccattttg acaaattcaa
     3181 ccccagtttc tgtttctagt tcattaacta atttaactat ttgtcgaata gaggcttttg
     3241 ctatatcatg gataccattt cgatgtaatg ccgtatctat tatttgtttt gaaattggaa
     3301 tattcacttt ttacaattat ttaaacgatt ctactttttt tactataaaa tatgctttat
     3361 aaggatttaa ctctatttct gtaacattgt gaaaaacaaa ccgttttggt tcggtagaag
     3421 gtaatactcg aataggttct ccatttaaat caatttgaac gggcaacgca aaatttgatt
     3481 gcacattagc ccaacgcatt tctatagtta cgatattatc ctttttataa gcaacatatt
     3541 caagtgtggg cacatgccta ctatataaat actgagtaaa ataccattct aaattcatac
     3601 ccgatttttg ctgtacaaaa tcagtaaaat cttttacggt tactatagaa tatgcccgag
     3661 tgttgtaata cgtttttaaa atatcaaaaa acaaactatc gttattaatt acataacgca
     3721 atccatgaag cgtccatgct cctttcatat acggatcggt atctttataa tcccaaaaat
     3781 taacatctct gggacctaca attggatttt tatttttaat atatcctgca tatttataca
     3841 tgtatcgttc agcttctttt acaccatatg tgtgttcaat aaacaaagct tccgaataag
     3901 tagcaaaacc ttcgtgaata aatatatcgg caaaatcgtc taccgatatt gagttgcccc
     3961 accattcatg tgccgattca tgtaaaataa tataatcaaa tcctaaatga tgtgttctga
     4021 aaccggttcc atatgctatt gctgtttgat gttccattcc ttcatatgga ctcccaacaa
     4081 gtttaaatcc ttctgttggc catggataag gaccatatat tgattcataa tatctaagaa
     4141 tattagaggt ttgttgaaat gttatttttg ttttttctaa atgctcgggc aatacataat
     4201 aatcaagagt tatggtagta tcaattcctt tatatttttc agaaaaatgt tcaaatttcc
     4261 caatatacaa tgtaacatta tagttgttta tagtatactt tgtttgccat gtaaacgtat
     4321 cttttccttt gtatgatttt tgttcaagta attttccatt cgaaacaacc gtaagaccct
     4381 gatgtacagt aacattcatg gtaacaccat attcgggttc atcacttaaa tggtctttac
     4441 atggccacca taaacttgct cctaccaact cgcaagcaac acctatccac ggatttttct
     4501 gttcatcttt tttccataca aaacctccct cccaaggtgg acgaggagct tctaaaggct
     4561 tacctttata atacattctt atagtttctg ttttccctat ttctaattga ggtatttgta
     4621 tgaaaacagc atcatatttt cgttcaaact taagtcgttt cccatgcaac acaatgctat
     4681 ccatggtcat atttttaaat aaatcaacct gcattttttc agtatgttgc attacagaaa
     4741 attgcatatc tacataccct tgaatagatt ttttttgagg atttacatca attgtaagtg
     4801 tataatgcaa tacattataa cacgttctaa aaggatttaa agcacctcgc aaactatcct
     4861 tttgcgtaaa ttttgggtat gtataatcaa ctgtttctgc tttcccaaca cgtgccgttt
     4921 tttgtgatga gcatgatact gcaaccaaaa cagtaacaaa aaaacatata tgtttacaaa
     4981 tattcatata ctatgttttt aaaatgtata tcctatacca acaagtaaat tcttattaaa
     5041 aatatctatt cctgcttgtg ccgataattt attatttgcc gcatacattc ctccaaacgt
     5101 agcagtatac cacatgccgt gtttaggaat tgtataagta aacaaactat tttcagtatt
     5161 tgtataataa gtacgttgag caacaaatcc cggagctgca tatacaaacc aatttcgtgt
     5221 tactgcacgt gctattccaa tattacacga aaatgaccta tacggtacta tcatttgcga
     5281 tactcccaaa ccttgtaatt gattacctgt tataacatat cgacctggta aatttgtttt
     5341 acattctata taatacgaaa ttctatcgag tgaattatgt ataaaaaaag aagcaccaat
     5401 atcatgaaca ttcatttggt acaatacaat agttgtaact cgtaatgaac gtttataaaa
     5461 atctgtggta agagaatcgt ttgtttgagc agtaaaaaag ttatattgag caaaacttgc
     5521 atgaatactt actaaacaca caacaataaa acctattact tgtttactca taatttcttt
     5581 tttttaacaa acatatacat ttttacaaaa ataattgcaa ttgtccgttg ggtttctttc
     5641 catgcaaaag cgaaaagtca gttttttgtg cttctttatc atatgcatgc aaatcaaaat
     5701 aaaatcgttg caatggcttg tgtatatttt tggaaggaat atactctgta ttttttacaa
     5761 attcactcac ctcatcgtta gtaaatactc gaatatcacc atattcgcca tcgtaccctt
     5821 cggtaacata tacctcaccg gcacgcattc gacgaattcc ttcggctaca aatgaattcg
     5881 aacattgtgc tatatcagaa atttcgcaac gtgtaagtat ctcaaattct gcccccaaat
     5941 gttgaatcat ttgcatatag attgctttta cttttttaga cgtttcgctt acattatatg
     6001 cttgagccaa taattcaggc aatgaaataa tatattctat cgattgccaa ttgtaacaca
     6061 tacttatatc gcgacggtct gccaattgca ttacacggtc cataaccccg aatgtatagg
     6121 gttttttgca tgttttgcat acaaatgcat gttgcaatac ctcaaccggc gtagcacata
     6181 caccacattt gcgatgcccg gaaaaatgat atttgcctgc ttgcggatat gtatcaatag
     6241 tacccacaaa ttgttttaat tctttatgtt gtaatgcatg tactatagca ttgtatgata
     6301 tatcgcaatc aaacacattg gcatttcttc ctaatttatc gggtgaatgt gcatcggaat
     6361 ttgaaagtaa agcatagcgg tcgagaaaac tacacatcca attcatagga gcatcggacg
     6421 ataaacccgt ttcaacagca aatatgtaag gttccatatc tccatagcat tctgcaatag
     6481 aatcaaaacc tgatttggcc ccaagagcag aaaaccacgg tgtccaaatg tgtgcaggaa
     6541 ctatatgaat atcaggagaa atgtctttgc atagtgccgc taaatcacgt gcatccatac
     6601 caataatagg acgaccatct gaagcaatat ttaaattacg ttgtataaac gcatgctgta
     6661 ctttggttac aatatcaaaa ttgggagccc acaaaatagt gtgaacttta cgagttttac
     6721 catttttttt gtaaatagaa ctaatttcgg cagtaagtac aaaacgcacc tgagcttgag
     6781 gaatagcttg agaatgttca acaaaatcgg acttaagcat aaacaaac
//
LOCUS       DAOTOW010000017         6817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9676_length_6817_cov_3.320172, whole genome shotgun sequence.
ACCESSION   DAOTOW010000017 DAOTOW010000000
VERSION     DAOTOW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9676_length_6817_cov_3.320172"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="P5243_00610"
     CDS             <1..1030
                     /locus_tag="P5243_00610"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRS17975.1"
                     /translation="VANHTSMVKNEHLVDMLIRNKVTIVKIFTPEHGFRGKADAGEAV
                     TNSVDPKTNIPIISLYGDKKKPSVADMQGIEIVVFDMQDVGARFYTYISTMHNVMEAC
                     AENGIDFWVLDRPNPNGFYVDGPVLNMQYKSFIGMHPVPIVHGMTVAEYARMIQGEKW
                     LAGGIQCKLHYVMCDGYTHKMKYIPPIAPSPNLPNITSIYLYPVLCLTEGTVLSCGRG
                     TDFPFQVVGHPKYKQTQFSFTPRSIEGASKNPPHKNVLCYGTDLRKYPVNTITQLPLD
                     IFIDAYKNYNGTEPFFTPFFLKVAGTAELQKQIEQGMTAQQIRNSWKQDLETFMKIRK
                     KYLLYADF"
     gene            1069..2358
                     /locus_tag="P5243_00615"
     CDS             1069..2358
                     /locus_tag="P5243_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRS17976.1"
                     /translation="MIQELQSKNKRIAVLGLGYVGLPLALEFAKKYSVIGFDINSERI
                     ELMKQHIDPSEELEKEAFDGTDIEFTSNPNDLSKAHFFIVAVPTPIDNHNIPNLRPLL
                     SATELVGKALKKGDYVVYESTVYPGATEEDCLPILQDISKLQLGVDFKLGYSPERINP
                     GDKVYTLTNILKIVSGSDAQALDVIANVYGSIITKGIHRTSSIKVAEAAKIIENTQRD
                     VNIALMNELSMIFKKMNINTYEVLEAAATKWNFYKVTPGLVGGHCIGIDPYYLTYKAI
                     QLKHHAQIITSGRAINDNMPIYLAKNILKEINNAGKVLKNSRVLIMGATFKENVSDIR
                     NSKIFDTVAELESFGIHVDVVDPYACPNEVLREYNCKLAQEPQGKYDVILIAVKHTEY
                     ENLSESYFKSIAAEPFILFDIKGIYRNAFPKARYFSF"
     gene            complement(2369..2530)
                     /locus_tag="P5243_00620"
     CDS             complement(2369..2530)
                     /locus_tag="P5243_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HRS17977.1"
                     /translation="MIQEIFVYIILSATIVYIVRSLYQSLKPHKRNNTCSGCNGCSLQ
                     HSCKQSCKE"
     gene            complement(2545..5016)
                     /gene="feoB"
                     /locus_tag="P5243_00625"
     CDS             complement(2545..5016)
                     /gene="feoB"
                     /locus_tag="P5243_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRS17978.1"
                     /translation="MSVLSELENGESAIISKVKGYGAFRKRITEMGFITGTKVTAIKK
                     APLQDPVEYELLGYRVTLRLSEAHNIEIVSEHEKPIDDDEPFEGLLTEEIIQKTVHKR
                     SKTIQVALVGNPNCGKTTLFNFATGKHERVGNYGGVTVDMKTAKLKHKGYTFHITDLP
                     GTYSISEYTPEELFVRKHIFNTMPDVVINVIDSSNLERNLYLTTQLIDMNIRIVGALN
                     MYDELEQKGDVFRYDELGKMIGIPFVPTVASRGKGIFELLEKIIDVYEEKEPYMRHVH
                     INYGQEINSSIEILKSEIKKNQQLVDVFHSQYVAVKLLEDDKAFARELEKIPDIQHII
                     ELGKKQRMILEKNFDERAETIMTDAKYSFIRGALRQTYFYSKEKQQTALNHGLDTVFT
                     HTWLGFPIFLLLMWGMFQATFSLGSYPMEWIDAGVGALGNFIERTMSQGMLRDLVIDG
                     IIGGVGGVIIFLPNIVILFLCISILEDTGYMARAAFIMDRIMRKIGLHGKSFIPLLMG
                     FGCNVPAVMATRTLENRKDRLLTMLIIPFMSCSARLPVYVLLISAFFPEKQGLVLLSI
                     YAIGVVLSVLVALLFKRIFFNKEEVPFVMELPPYRVPTLRNTLNHMWGKSVQYLQKMG
                     NIILVASIVIWALGYFPKHIEFSQDYDALIQLVTANESLSDTEKDEHIKQLFIAKESE
                     RMEKSYIGQMGHFIAPAIQPLGFDWKIGVSIVTGLAAKEIVVSSMGVLYQSDLNADEA
                     SVSLQQKLQEQTHAYGPRKGEKVFTPLVAFTLMLFVLIYFPCVAVIAAIKREANWKWA
                     SFTMVYTTVLAWLLALATYQIGSIL"
     gene            complement(5037..6161)
                     /locus_tag="P5243_00630"
     CDS             complement(5037..6161)
                     /locus_tag="P5243_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRS17979.1"
                     /translation="MKKLLIACILIYSIHGAAQQSDSIASAISVDASYIGDFVYNVHG
                     GIQQGAAFLGMASLGIMLETNKLGLWKDGTLYIHGKSLHGKSPSANLVGDFQVISNID
                     GGEHSYLHECWYSQSFQNIHVTVGLQDANVELATSELGSHFINSSFGIPSLIANNVPT
                     PIFPLTNVGVTIKAQLHKKVDVVAALYDGKPTHFDINRYNTDWELQKEDGVLSFVEFQ
                     YSSSQSNMYKLGYFNHSGLQEFNDSTRNFEQVFSRNYGLYGIADYILWSNIEQEKSLG
                     IFTQIAASPKSINRHSMYVGGGLVYTGIGVARSVLGLAIAHARFNNKTSETAIEASYK
                     AHIFSNVSIQPDVQYIIHPMGNEKRIPHALAGILRVIVEL"
     gene            6459..>6817
                     /locus_tag="P5243_00635"
     CDS             6459..>6817
                     /locus_tag="P5243_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRS17980.1"
                     /translation="MNEQEKIILQKLLHPNLKEKAFEELLEMYQKKLYWYIRKIVLTH
                     DDANDVLQNTFIRIFNNIESFKGKSSLKTWIYTIAHNEAIRYISQKKIHVSLHDISNQ
                     YMNTLKQDVFFDGEKLS"
BASE COUNT         2344 a         1204 c         1013 g         2256 t
ORIGIN      
        1 agtagccaat catacgtcta tggtaaaaaa tgaacattta gtagacatgc ttattcgcaa
       61 caaagttact attgtcaaaa tttttacacc cgaacatgga tttaggggta aagccgatgc
      121 cggcgaagct gtaacaaatt cagttgaccc caaaactaat attcctataa tttcattata
      181 tggcgataaa aagaaacctt ctgttgccga tatgcaagga atagaaatag ttgtatttga
      241 catgcaagat gtaggagcac gtttttatac atatatatct actatgcaca atgtaatgga
      301 agcatgtgcc gaaaatggaa tagatttttg ggtattagac agaccaaatc caaatggatt
      361 ttatgttgat ggtcctgttt taaacatgca atataaatca tttataggca tgcacccggt
      421 acctatagta catggtatga cagtagccga atatgctcgt atgatacaag gtgaaaaatg
      481 gcttgcagga ggcatacaat gtaaacttca ctatgtaatg tgcgatgggt atacacacaa
      541 aatgaaatat ataccaccaa tagcaccttc gccaaattta ccgaatatta catctattta
      601 tttgtatcct gtattatgtt taaccgaagg aactgtgtta agttgtggaa gaggcactga
      661 ctttccgttt caagtagtag gacaccccaa atataaacaa acacaatttt cgtttactcc
      721 tcgcagtata gaaggagcta gtaaaaatcc tcctcacaaa aatgtattgt gttacggtac
      781 tgatttacgc aaatatccgg taaacacaat cactcaattg ccacttgata tttttataga
      841 tgcatataaa aattacaatg gcacagaacc gttttttaca ccattttttt taaaagtagc
      901 cggaactgca gaattacaaa aacaaataga acaaggaatg acagcacagc aaataagaaa
      961 ttcatggaaa caagaccttg aaacctttat gaaaattaga aaaaaatatc tgctttatgc
     1021 tgatttttag aaagctatga agtatatttg cactttgaag aatacgatat gatacaagag
     1081 ttacaatcaa aaaataaacg tatagcagtt cttgggttag gatacgttgg gctgccacta
     1141 gcattagaat ttgcaaaaaa atattcagtt ataggatttg atatcaattc agaacgaatt
     1201 gaattgatga aacaacatat agatcccagt gaagaattag aaaaagaagc ctttgatggt
     1261 accgatatag aatttacatc gaatcctaac gatttgagta aggctcattt ttttattgta
     1321 gctgtaccaa ctcctatcga caatcataat attcctaatt tgcgacctct tttgtctgca
     1381 accgaactag ttggtaaagc acttaaaaaa ggtgattatg ttgtgtatga atctactgtg
     1441 tatcccggag ctaccgaaga agattgttta ccaatattac aagatatatc taaattacag
     1501 ttaggtgttg attttaaatt aggatattca ccggaacgaa taaatccggg cgacaaagta
     1561 tatactctta ccaatattct taaaattgta tcgggtagtg atgcccaagc tcttgatgta
     1621 attgccaatg tttatggctc aattataact aaaggtattc atagaacaag tagtataaaa
     1681 gtagccgaag ctgcaaaaat tattgaaaat actcaacgcg atgtaaatat agcattgatg
     1741 aatgaactct caatgatatt taaaaaaatg aatattaata catacgaagt acttgaggct
     1801 gcagctacaa aatggaattt ctacaaagtt actcccggtt tggttggcgg gcattgtatc
     1861 ggtattgatc catattacct tacatacaaa gctattcaat taaaacatca tgcgcaaatt
     1921 ataacttcgg gacgtgccat aaatgataat atgcctatat atttggcaaa aaatatttta
     1981 aaagaaataa ataatgccgg taaagtactc aaaaattcac gggtattaat aatgggagct
     2041 actttcaaag aaaacgtaag tgatattcgt aactcaaaaa tatttgatac cgttgccgaa
     2101 ttagaatcat ttggtattca tgtagatgta gttgatccct atgcttgtcc taatgaagta
     2161 ttgcgtgaat ataattgcaa attagcacaa gaacctcaag gcaaatatga tgtaattttg
     2221 attgcagtaa aacatacaga atacgaaaat ttatcagaat catattttaa atctattgca
     2281 gcagaaccat ttattttatt tgatattaaa ggtatttatc gaaatgcttt ccctaaagct
     2341 cgatatttca gtttttaata ttataacttt attctttaca actttgttta catgaatgtt
     2401 gcaacgaaca tccgttgcaa ccactacatg tgttatttct tttatgcggt ttaagtgatt
     2461 gatacaacga acgcacaata tacactattg ttgctgatag aataatatat acaaaaattt
     2521 cttgtatcat tgttttttca atttttacaa tatactacca atttggtatg ttgctaatgc
     2581 caacagccat gccaacacag tagtgtaaac cattgtaaat gaagcccact tccaattagc
     2641 ctcacgtttt attgcagcta ttaccgcaac acacggaaaa tatattaata caaacaacat
     2701 aagagtaaat gctaccaaag gagtaaacac tttttcacct ttacgcggtc cgtatgcatg
     2761 tgtttgttct tgtaattttt gttgtaaact taccgaggct tcatcggcat tcagatctga
     2821 ctgatacaaa acccccatag aacttacaac tatttcttta gccgctaaac cggttacaat
     2881 acttacacct attttccaat caaatcctaa aggttgaatt gccggtgcta taaagtgccc
     2941 catttgtcct atatatgatt tttccatgcg ctcagattct tttgcaataa ataattgttt
     3001 tatgtgctca tctttttcgg tatcagataa cgattcgttt gctgtaacca attgtatgag
     3061 tgcatcataa tcttgtgaga attctatatg tttcgggaaa tatcctaaag cccatattac
     3121 tatagaagca accaaaatta tatttcccat tttttgtaaa tattgcacac ttttgcccca
     3181 catatgatta agagtattac gtaacgtagg aactctatac ggtggtaatt ccataacaaa
     3241 cggtacttct tctttattaa agaatattcg cttaaacaat aaagctacaa gtaccgataa
     3301 tactacacca atagcatata ttgaaagaag aactaatcct tgtttttcag gaaaaaatgc
     3361 agagattaac aacacataca ccggtaatcg agcactacac gacataaacg gtataataag
     3421 catggttaat aatcggtctt ttctgttctc caatgtacgt gtagccatta ctgccggaac
     3481 attacaacca aatcccataa gaaggggtat aaatgacttt ccatgcaatc ctattttacg
     3541 cattattcta tccataataa aagcagcccg agccatatac cctgtatctt caagtataga
     3601 aatacataaa aatagaatta caatatttgg taaaaatata attactcctc ctactccgcc
     3661 aataattcca tctataacca aatcgcgaag catgccttgg ctcatagtac gttctataaa
     3721 attacctaaa gctcccacac ctgcatctat ccattccatt gggtaactac caagtgaaaa
     3781 tgttgcttga aacatacccc acataagcaa caaaaatatt ggaaatccta accacgtatg
     3841 agtaaaaaca gtatctaacc catgatttaa tgctgtttgt tgcttttctt tagaatagaa
     3901 atatgtttga cgcaatgcac ctcgaataaa actatatttt gcatcggtca ttatagtttc
     3961 ggcacgttca tcaaaatttt tctctaaaat cattcgttgt ttttttccta attcaattat
     4021 atgctgtatg tccggtattt tttccagctc acgagcaaat gctttatcat cttcaagtaa
     4081 ttttactgca acatattggg aatgaaacac atcaacaagt tgttgattct tttttatctc
     4141 tgattttaaa atttctattg aagaattaat ttcttgacca taatttatgt gtacatggcg
     4201 catataaggt tctttttctt cgtacacatc aattattttt tcgagcagtt cgaaaattcc
     4261 tttaccacgc gaagcaacag ttggtacaaa agggattcca atcattttac ctaattcatc
     4321 ataccgaaac acatcgcctt tttgttccaa ttcatcatac atattaagag ctcccactat
     4381 acgtatattc atatctataa gttgagtggt caaataaaga tttcgttcta aatttgaact
     4441 atctattaca ttaattacca catcgggcat agtattaaaa atatgtttac gaacaaacaa
     4501 ttcttcggga gtatattctg atatagaata tgttccgggt aaatcggtta tatgaaatgt
     4561 atatccttta tgtttgagtt ttgctgtttt catatctaca gttacaccac cgtaattgcc
     4621 aacacgctca tgctttccgg tagcaaaatt aaataaggtg gttttaccgc aatttggatt
     4681 accaaccaat gctacttgaa tagtttttga acgcttgtga actgtttttt gaattatttc
     4741 ttcggtaagc aatccttcaa atggctcgtc atcatctata ggcttttcat gttccgaaac
     4801 tatttctata ttatgagcct cgctcaatcg tagcgtaact ctataaccta ataattcata
     4861 ttcaaccgga tcttgcaaag gtgctttctt aatagcagta actttagtgc cggtaataaa
     4921 gcccatttca gtaattcgtt tgcgaaatgc tccataccct ttaaccttac ttataattgc
     4981 actttcgcca ttttctaatt ctgataatac tgacattgta tatactatat atatttttat
     5041 aattctacta taacccttaa tattcctgcc aatgcatgag gtattctttt ctcattgccc
     5101 ataggatgta ttatatattg tacatcgggt tgaatactaa catttgaaaa aatatgtgct
     5161 ttataacttg cttctatagc cgtttcactt gtcttattat tgaatcgagc atgtgctatt
     5221 gccagcccca acacagaccg ggcaacacca attccagtat atactaaacc tcctccaaca
     5281 tacatagaat gcctattaat agattttgga cttgcagcaa tttgtgtgaa aatcccaagt
     5341 gatttttcct gttctatatt actccacaat atataatcag ctataccata caatccgtaa
     5401 tttcgtgaaa acacttgttc aaagttacgt gttgaatcat taaattcttg taaaccggaa
     5461 tgattaaaat atccaagctt atacatattt gattgagaag atgaatactg aaattcaaca
     5521 aaacttaaaa caccatcttc tttttgtaat tcccaatcag tattgtatct gttaatatca
     5581 aaatgtgtag gtttgccatc atacaatgca gctactacat ctactttttt atgcaattga
     5641 gcttttattg taacacctac attagtaaga ggaaaaattg gtgttggtac attattggca
     5701 ataagcgatg gtataccgaa tgaactatta ataaaatgag aaccaagttc gctagttgct
     5761 aattctacat ttgcatcttg caacccaaca gttacatgta tattttgaaa cgattgtgaa
     5821 taccaacatt catgtaaata ggagtgttct cctccatcaa tatttgaaat tacttgaaaa
     5881 tctccaacca aatttgcact aggtgatttt ccatgaagcg attttccgtg tatgtataat
     5941 gttccatcct tccataaccc taatttatta gtttctaaca taatgcctag cgaagccata
     6001 cctaaaaaag cagccccttg ttgtatacct ccgtgcacat tgtatacaaa atcaccaata
     6061 taagaagcat ctactgaaat agcagatgca atactatctg attgttgagc tgcaccatgt
     6121 atactgtata taagtataca tgcaattaat aatttcttca tattgtatta aatttacttt
     6181 gcaaagtagc acataataaa cacgtactac aatccctaaa aatagggatt tttaacatct
     6241 tcattcgtaa aattttgatt gcattattat tgctttttgc tttgtttttt atatctctaa
     6301 agattatacg agtaagcata ttttcctata attccataga ttttaaatta caattgctga
     6361 cgcatatatc aatttaatta tttttataga aaatttttga tacacaaaac aactattttt
     6421 tatttcaaag gtctacatag aaataatgtg atacaaaaat gaatgaacaa gaaaaaatta
     6481 ttctacaaaa attgttacat ccaaatttga aggaaaaagc atttgaagaa ttactggaaa
     6541 tgtaccaaaa aaaactatat tggtatattc gtaaaattgt tcttactcat gatgatgcta
     6601 atgatgtttt acaaaataca tttatacgaa ttttcaataa tattgaaagt tttaaaggta
     6661 agagttcttt gaaaacatgg atatacacaa ttgctcataa tgaagctatt cgatatattt
     6721 cacaaaaaaa aatacatgtt tcattacatg atatctcaaa tcaatatatg aacactttaa
     6781 aacaagatgt tttttttgat ggagaaaaac tctcgga
//
LOCUS       DAOTOW010000018         6780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9738_length_6780_cov_3.310335, whole genome shotgun sequence.
ACCESSION   DAOTOW010000018 DAOTOW010000000
VERSION     DAOTOW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9738_length_6780_cov_3.310335"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="P5243_00640"
     CDS             <1..1579
                     /locus_tag="P5243_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17981.1"
                     /translation="QSMQMQIEFIAFSFVCLPNETSPGYIIAKKNNAYLEYTNTNKTM
                     VLIGLNIPHPQIVPRSSPLFNNYSVTHYKQYFKYFVQAAGAHCNFIRVALVGSYTSAP
                     EFDKILNYNQRQPYMFEFDTLLHFAEKNNIYFQIIGDSFGPYVISWDLNPYQYISGIR
                     NPLDVFTSDVAREIYKQRIRYIYSRWGYSTSFCMYQIINEIDQIVYKNQKLSSEIIYN
                     WVSDIANYITEDLQYPIIVTTNFAGFDSFPYVFALDNIDVTTIHYYGKNRNHDLVALY
                     SRVQNEWNLFKKPVIIDETGGNDSYGNIDAQSDLLYKNRLFSTVFSGGAGAAVQYYWN
                     VYMSQEGDVRSSYVWYNKLLNDYLNILDSLHIYNYNYLQFSPQRFPQFESKELYNVPL
                     YDTLFSLVEQFALCDSSKQIILGWVHNRSHYWANEVSDSLLYPKDDDYTKKPILIDYK
                     NPNVLVGEYVIMYDLLPSMSYEISWYSTRKSQEKITKINEGILQFTTNSEGVSQKIYP
                     PFSAEEFDFLFIAKKIE"
     gene            complement(1587..3818)
                     /locus_tag="P5243_00645"
     CDS             complement(1587..3818)
                     /locus_tag="P5243_00645"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4859 domain-containing protein"
                     /protein_id="HRS17982.1"
                     /translation="MKAILVSIFLIISSISFSQKQIYIPTFITNTGMNLNDPASQWCY
                     ARSTETENWIIFWEAGFGNDPSKATGNYTVNMEALKICAEKSFLHFRDSLRMVVKGSS
                     VTDTYKQMIFILYSTEWAAYGSGQDNKVGTLHVNPAAANIAVVLAHEIGHCFQYLTGC
                     DSPLGGYRYGFGPNASGGNGFWEQCAQWKAFKAYPNEQFKAHDFTQYIQYNHLNILHE
                     TPRYANYFLPDFWTFKQNQYFIGRLWRDSRKPEDPVETYKRLTAINQEQFNDQMYEHA
                     ARLTTWDIPSIREYGANYIFSRKQVQMTQSANNYWTIDSVVCIENYGYNSIQLNVPTS
                     ETTVNVTFKGLAGTAGFRSLNVTKAGWRFGFVALLNDGTRVYSDMATAKYTNGMNPTT
                     TLSFRCPNNCKALWLVVSGAPQEHWRHAWDDDNSNDEQWPYQVQFTETNLFGFYTNPI
                     HNESLAYTLYMQPMTDYTPTPVTLDRNKICHAFAMPPDSIAKLLGIAIRYYGVNPDGT
                     LNANSTATAPGHWYNKSGQTVAWGTNSFVFSELSLQNLIAKIGQYPNKLVVGDVITIR
                     QALIYTKSANETARIDLSFTIIISPPPITQTIQLQKGWNLISLFVRPVDSTITSIFAG
                     KDVQEIKTASAFWYKGQNLAFNSLNTMSAGQGYLVKMNAATSISITGVPLSGPAQYPV
                     NTLKAGWNLLGCPYQNTTPFTNHFNATNCQIIKNFDGFWQPGGTTNSITNFEPGKAYY
                     IKL"
     gene            complement(3825..>6780)
                     /locus_tag="P5243_00650"
     CDS             complement(3825..>6780)
                     /locus_tag="P5243_00650"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17983.1"
                     /translation="HHIRNLLLISLLLFLSITVFSQRQMENLDRGVFAIRSGTGNLVS
                     WRLLGLEYKTNIGFNLYRNGTKVNATPITTKTNYFDAGAATTAQYYVRAVVNNVEQPA
                     SKTVSVLNAKFLRIPLNVPAGGTTPDAVAYTYSPNDCSAGDVDGDGEYEIIVKWDPSN
                     SKDNSQSGYTGNVYLDAYKLNGTFLWRIDLGINIRAGAHYTQFMVYDLDCDGKAEIAC
                     KTAPGTKGGDGQFLKKGPAASDNDAADYRNTSGYILSGPEYLTIFNGQSGVEMQTIYY
                     NPRRHPTTENPTATQLNTEWGDNYGNRVDRFLAGVAYLDGKKPSIVFTRGYYTRTVLV
                     AFDWNGTNITQKWIFDTGNNASNAYYGQGNHNLAVGDLDGDGFDEIQFGSMAVDHDGK
                     GLYTTGFGHGDAGHLSDMDPSRPGLEFFSPHEEANGTTKPGVDLRDPKTGQVLWRKEA
                     AADIGRGICIDIDPNYYGYECWASGNLGVYNIKGDLIYTNFPQSTGGSQSINFGIWWD
                     GDLLREILDKVVINKFNYTSKATDRLFTVYNEGGASDINGTKSNPCLVADIFGDWREE
                     VIFRAATNDALLLFPTTYESTEMLYTLMHDPTYRLSIAWQNVAYNQPPNPGFYLGAGM
                     PAAPVPNIVYVNAGTTPVNDCNGTPNGSAYIDSCGTCVGGTTGKTPCVKDCNNVWGGS
                     ATIDICGVCVGGTTGKTACSTIQAETSCNYVGTIDNNNAGFTGSGFVNVSNQIGSHVT
                     ISCKAATAKTETIYIRYSNGTTATRNCEIMVNNQAVVANQSFASTTNWTTWTTIPITI
                     QAKQGINTIKITSLTADGAPNIDAIGVSSNITEVLCLTQTIQLVQGWNLLSMYIQPAD
                     STITSIFAGKDVQEIKNASAFWLKGQNVAFNSLNTMSAGQGYLVKMNAATSISITGVP
                     LSGPAQYPVNTLKAGWNLLGCPYQNTTPFTNHFNATNCQIIKNFEGFWQPGGTTNSIT
                     NFEPGKGYYIKK"
BASE COUNT         2071 a         1195 c         1235 g         2279 t
ORIGIN      
        1 acaatctatg caaatgcaaa ttgagtttat tgcattttca tttgtatgcc tacccaatga
       61 aacatctccg gggtatataa ttgcaaaaaa aaataatgca tatcttgaat atacaaatac
      121 aaacaaaaca atggtattaa ttggcttaaa tattcctcat ccccaaattg taccaagaag
      181 ttcaccttta tttaataatt attcggtaac acattataaa cagtatttta aatattttgt
      241 gcaagcagcc ggggctcact gtaattttat tagagttgct ttagtaggaa gttatacttc
      301 ggcacctgaa tttgataaaa ttttaaatta taatcaacga caaccgtata tgtttgaatt
      361 tgatacatta ttacattttg cagaaaaaaa taatatctat tttcagataa ttggagattc
      421 gtttggccct tatgtgataa gttgggattt aaatccgtat caatatatat caggaattag
      481 aaacccttta gatgtattta cttccgatgt tgctcgtgaa atatataaac aaagaattag
      541 atatatatat tcacgatggg gatatagcac atcattttgt atgtatcaaa ttataaatga
      601 gatagatcaa attgtttata agaatcaaaa attatcatct gaaattatat ataattgggt
      661 ttctgatatt gctaactata taactgagga tttgcaatat ccaataattg taacaacgaa
      721 ttttgccgga tttgattcat ttccatatgt gtttgcatta gataatatag atgttacaac
      781 aattcattat tacggaaaaa atagaaatca tgatttggta gcattatatt ctcgagttca
      841 gaatgaatgg aatttattta aaaaaccggt tattattgat gaaacaggtg gaaatgattc
      901 gtatggtaat attgatgcac aaagtgattt gctgtataaa aacagattgt tttctactgt
      961 tttttcggga ggggcaggag cggctgtgca atattattgg aatgtttata tgtcacaaga
     1021 aggagatgtt aggagttcat atgtgtggta taataaatta ctaaatgatt atttgaatat
     1081 tttagattca ttgcatattt ataattataa ttatcttcaa ttttcgccac aacgttttcc
     1141 tcaatttgaa tcaaaagaat tatataatgt gcctttatat gatactcttt tttctttggt
     1201 agaacaattt gctttatgtg attcttcaaa acaaattatt cttggatggg ttcataatcg
     1261 ttctcattat tgggcaaatg aggtttcaga ttctttacta tatcccaaag atgatgatta
     1321 tacaaaaaag ccaatactaa tagattataa aaatccaaat gtattagtgg gagagtatgt
     1381 tataatgtat gatttattgc cttcaatgag ctatgaaatt agctggtatt ctactcgtaa
     1441 atcacaagaa aaaattacaa aaataaatga ggggattttg cagtttacaa caaattcaga
     1501 aggtgtaagt caaaaaatat atcctccatt ttctgctgaa gaattcgatt ttttatttat
     1561 tgctaaaaag atagagtagt caaactttat aacttaatat agtatgcttt acccggttca
     1621 aaattggtaa tagaattggt agttcccccg ggttgccaaa atccatcaaa attttttatg
     1681 atttgacaat tggttgcatt aaagtgattg gtaaatggag tagtgttttg atatgggcaa
     1741 ccaagtaaat tccatcctgc ttttagcgta tttacaggat attgtgcggg gcctgaaaga
     1801 ggcacaccgg taattgaaat tgatgtagcc gcattcattt tcactaaata tccttgtcct
     1861 gccgacatag tattgagtga attaaatgct aggttttgcc ctttatacca aaaagcagaa
     1921 gcggttttta tttcttgcac atcttttcct gcaaaaatag atgtaatagt ggaatctaca
     1981 ggacgaacaa acaatgaaat taaattccat cctttttgta actgaattgt ttgtgtaatt
     2041 ggcggaggcg aaattataat tgtaaatgaa agatcaatgc gagctgtttc attcgcgctt
     2101 ttcgtatata taagagcctg acgaatagta ataacatctc caacaactaa tttgttggga
     2161 tattgaccga tttttgcaat aagattttgc aatgataatt cggagaatac aaacgaattg
     2221 gtaccccatg ccactgtttg ccctgattta ttgtaccaat gtccgggagc cgtagctgta
     2281 gaatttgcat tgagcgtgcc atcgggattt acaccataat accgaatagc tattcctaat
     2341 aattttgcaa tactatcggg aggcatagca aaagcatggc aaattttatt gcggtcgagt
     2401 gttaccggtg taggtgtata atcggtcata ggttgcatat ataaagtata cgcaagagat
     2461 tcattatgaa taggattggt ataaaatccg aacagatttg tttcggtaaa ctgcacttga
     2521 tatggccatt gttcgtcgtt tgaattgtca tcatcccatg catgtcgcca gtgttcttgt
     2581 ggtgcacctg aaactacaag ccacaaagct ttgcaattgt ttggacagcg aaatgaaagt
     2641 gttgttgtag gattcatacc attggtatat tttgcagttg ccatatcgct atatacccgt
     2701 gtgccatcgt tgagcaatgc tacaaatcca aatcgccaac cggccttagt aacattgagc
     2761 gaacgaaacc cggctgtgcc agctaaacct ttgaatgtaa catttacagt ggtttctgaa
     2821 gttggcacat tgagttgaat gctattataa ccataatttt ctatacatac cacagaatct
     2881 attgtccaat aattattggc agactgggtc atttgtactt gtttgcgcga gaaaatataa
     2941 tttgcaccat attctcgtat cgaaggtata tcccatgtgg ttaaacgagc agcatgctca
     3001 tacatttgat cgttgaattg ttcttgattt atagctgtaa gtcgtttata tgtttcaacc
     3061 ggatcttcgg gtttgcgaga atctcgccac aatctgccaa taaaatattg attttgtttg
     3121 aatgtccaaa aatcgggaag aaagtaattg gcatatcgtg gggtttcgtg aagaatattg
     3181 aggtgattgt actgaatata ttgtgtaaaa tcatgagctt tgaattgttc atttggatat
     3241 gctttgaatg ctttccattg tgcacattgc tcccaaaaac cattaccgcc gcttgcatta
     3301 ggtccaaatc catatcgata gccgccaaga ggcgaatcgc aaccggtaag atattgaaaa
     3361 caatggccaa tttcatgagc caaaacaact gcaatattag cagctgcagg atttacatgc
     3421 agtgttccta ctttattgtc ttgccccgaa ccataggcag cccattcggt gctgtacaat
     3481 ataaaaatca tttgtttgta tgtatctgta accgacgaac cttttacaac catacgtaaa
     3541 gaatcgcgaa aatgaagaaa acttttttca gcacatattt taagtgcttc catattaacg
     3601 gtataatttc cggtagcttt cgatgggtcg tttccaaatc ctgcttccca aaaaattatc
     3661 caattttcgg tttcagtact tcgagcataa caccattgac ttgcaggatc gttcaaattc
     3721 attcctgtat ttgtaataaa tgtaggaata taaatttgtt tttgactaaa acttattgag
     3781 cttattatta ggaatatact tactaatatt gctttcataa ggaattattt tttaatataa
     3841 tatcctttac ccggttcaaa gttggtaata gaattggtag ttcccccggg ttgccaaaat
     3901 ccttcgaaat tttttatgat ttgacaattg gttgcattaa agtgattggt aaatggagta
     3961 gtgttttgat atgggcaacc aagtaaattc catcctgctt ttagcgtatt tacaggatat
     4021 tgtgcggggc ctgaaagagg cacaccggta attgaaattg atgtagccgc attcattttc
     4081 actaaatatc cttgtcctgc cgacatagta ttgagtgaat taaatgctac gttttgtcct
     4141 ttgagccaaa aagcagatgc atttttaatt tcttgtacat cttttcctgc gaaaatactt
     4201 gttattgtag aatctgcagg ttgtatatac attgatagta aattccaccc ttgtacaagt
     4261 tgaatagtct gagttaaaca taaaacttcg gtaatatttg agcttacacc tatagcatct
     4321 atgtttggtg cgccatcagc ggtaagcgat gtaattttta tggtatttat tccttgtttt
     4381 gcttgtattg taataggaat tgtagtccac gtagtccaat tggtagttga tgcaaaactt
     4441 tgatttgcaa ctactgcttg attgtttacc attatttcgc aattacgtgt tgctgtagta
     4501 ccatttgaat agcgaatata gatagtttct gtttttgcag tggcagcttt gcacgaaata
     4561 gttacatgag aacctatttg gttcgaaaca tttacaaaac ctgaacctgt aaatcctgca
     4621 ttattgttat cgatagttcc aacataatta cacgaagttt ctgcttgaat tgtactgcaa
     4681 gctgttttgc cggtagtacc tccaacgcaa acaccacaaa tatctatagt tgcacttcct
     4741 ccccacacat tattgcaatc ttttacacaa ggggttttgc cggtagtacc gcctacacat
     4801 gtgccacaac tatcaatata cgccgaaccg ttgggtgtgc cattacagtc gtttacagga
     4861 gttgtacccg catttacata tacaatattt ggaaccggag cagcaggcat tcctgcacct
     4921 aaataaaaac ccggattggg tggttgatta tatgccacat tttgccatgc aatagaaagt
     4981 ctatatgtag ggtcgtgcat aagtgtatac agcatttctg ttgattcata ggtggttggg
     5041 aataaaagta atgcgtcgtt tgtagctgct ctaaaaatta cttcctcacg ccaatcacca
     5101 aaaatatcgg ctactaaaca aggatttgat tttgtaccgt ttatatcact tgcgccacct
     5161 tcgttgtata cagtaaacaa tctgtcggta gcttttgaag tatagttaaa tttgttgatt
     5221 actactttat ctaaaatttc gcgaagcaaa tcgccatccc accatatacc aaagttgatt
     5281 gattgacttc ctccggtaga ttgggggaaa ttggtgtaaa tcaaatcacc ttttatgttg
     5341 tacacaccta agttacccga agcccaacat tcatagccat agtaatttgg gtcaatatct
     5401 atacaaatac ctcgtccaat atctgcagca gcttcttttc tccacaatac ttgtcctgtt
     5461 ttggggtctc gcaaatcaac accgggtttg gtagttccgt ttgcttcttc gtgaggcgaa
     5521 aaaaattcaa gtcccggccg tgacggatcc atatccgata aatgtcctgc atcgccatgt
     5581 ccaaatcctg ttgtgtataa acctttacca tcgtgatcta cagccatgct tccaaattga
     5641 atttcatcaa acccatcacc gtctaaatcg ccaacagcta aattatgatt cccttgtccg
     5701 taatatgcat ttgacgcatt gttgccggta tcaaatatcc atttttgagt gatatttgta
     5761 ccattccaat caaatgcaac taacactgtt cgcgtataat atcctcttgt aaaaacaata
     5821 cttggttttt tgccgtcgag gtatgctaca cctgccaaaa atcggtcaac tctattacca
     5881 taattgtcac cccattctgt attgagttgt gttgcagtag gattttcggt agttgggtgt
     5941 ctgcgtggat tataatatat cgtttgcatt tcgactccgc tttgtccgtt gaaaattgta
     6001 agatattcgg gaccggataa aatataaccc gaagtatttc tataatcggc agcatcgtta
     6061 tcgcttgctg caggtccttt ttttaagaat tgaccatcgc cacctttggt accgggagca
     6121 gttttacatg caatttctgc tttaccgtcg caatctaaat cgtataccat aaactgagtg
     6181 tagtgtgcac ctgcacgtat attgattcct aaatctatgc gccataaaaa tgtgccattt
     6241 agtttgtatg catcaaggta tacattaccg gtatatccac tttgtgaatt atctttggaa
     6301 tttgaagggt cccattttac aataatttca tattctccgt caccatccac atcacctgcc
     6361 gaacaatcgt tgggagaata tgtgtatgct acggcatcgg gtgttgtacc tccggcaggt
     6421 acattgagag gaatacgtaa aaattttgca ttaagaaccg aaacagtttt cgaagctggt
     6481 tgttctacgt tgtttacaac tgctcgcaca taatattgag ctgtagttgc agcacctgca
     6541 tcaaaataat ttgttttggt agttattgga gtagcattta ccttggtgcc attgcgatat
     6601 aagttgaatc caatatttgt tttatactct aaacctaaca aacgccaact aactaaatta
     6661 cctgttcccg aacgtatagc aaaaacgcca cgatcgagat tttccatttg tcgttgagaa
     6721 aataccgtaa ttgataaaaa taataataaa ctaatcaaca ataaattacg gatatgatgt
//
LOCUS       DAOTOW010000019         6686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9900_length_6686_cov_3.096064, whole genome shotgun sequence.
ACCESSION   DAOTOW010000019 DAOTOW010000000
VERSION     DAOTOW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9900_length_6686_cov_3.096064"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="P5243_00655"
     CDS             complement(<1..208)
                     /locus_tag="P5243_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRS17984.1"
                     /translation="MKTTVVQSMNKQINAELYSSYLYLSMAAWANSKGYKGIANWLQI
                     QSKEEYAHAMKFYAYLEERNQKPEL"
     gene            complement(510..2900)
                     /locus_tag="P5243_00660"
     CDS             complement(510..2900)
                     /locus_tag="P5243_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRS17985.1"
                     /translation="MKSILITCAFCFIYICNAYSQQYIEHKGHEHFFSGINLAWMQFG
                     NDLTQFNPETFTAIMKNVSDAGGNSVRWWIHVDGRSTPTFANDTVIGISEQALNNLET
                     ALDIAAEHGIVISLCLWSFDMMHKKKDTETDIYANNRLRINRRLLEDSAASMAYIKYA
                     LIPMVERVKGHTAILCWEIFNEPEGMTSIANWPLIEKVDMKYIQRFTNWCAGAIHRTD
                     PQAKVSNGSWNAIATATLPNIYSSKNYYSNAELFDKGGDADGYQDFYMLHYYADYYGD
                     KYSPFHNPVQYWQLDKPLLIGEFAALGIIRQPGKPFAPSSQLTTTQAMSYLIDNGYAG
                     GWGWTYTGHDGNGNLNDMKYVLDSLKKTYPNLITIPRDPNHNYAPKNIARVKDTILFK
                     NSNPIPQYINLNQFFEDEKQLSYTINSDGPAIATIVNDSILAISVLPDSIGLSKFIIT
                     GTDIGGKTARSQWNVVVRDSIIQSPNKLLYAYVTASSEEDAIKRIPLCNDGDKTTRWS
                     SIYNNNEWIEFDMLEQQTISRMKLFWEAAYGDQYEIRVSSNKQDWTTVFNIGQGQKSE
                     VNIVFEPISCRYIQILFKKRATGWGYSLFEVEAYENNGSSENVPPIANTSPTTISFDA
                     YYDLHYVFPRTKFSDANNDVLTLTASMPNGSALPNWLTFNPYTYEFIANTTIADTGTY
                     TIRVTATDFFNQSAYYDILFKITNPNSSIQKSIQTIALYPNPTSNSLCYFELPYAYSE
                     TALITITNSMGKIVSNQIIQCTKGQGEISLHNLTKGVYFITITDTINSYKQHIIVK"
     gene            3264..>4363
                     /locus_tag="P5243_00665"
     CDS             3264..>4363
                     /locus_tag="P5243_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRS17986.1"
                     /translation="MKKSLLVLLLLIVVFTQSFSQIKINEVQSSNTKTIKDSDFQEYV
                     DWIELYNPGTSSVNIGGYYLTDDKDEPRKWQIPTGTSIAAKGYLLIWCDGKNINRHTN
                     YKLGSDGERIVLYSNTMLFQDSIKFPEIATDFTYGRTVNGAGEWALLTAPTPGATNVS
                     TVVKGLAPKPTFNIQGGFYTSNQSVTLSTTIPGAVIRYTTDGSEPTASSPIYTGAITA
                     QKTTKTTQKYGNNRLNKTGIQKYGWPSSLTYPTGYYDGTREYGFVIKAKVFHPDYLPS
                     YTAGQTYFINMRKPTLPVVSITTDFNNFFSADTGIYIQGTNGISNGYVTANWYQDWER
                     KGHIEYFDATGSRKFGVSVGISTSGAVSRSYD"
     assembly_gap    4364..4373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4397..>6686
                     /locus_tag="P5243_00670"
     CDS             4397..>6686
                     /locus_tag="P5243_00670"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRS17987.1"
                     /translation="MRNKYETGTMEYQLFGSDGLSSYKSIMLRNSGNDWEMGNFARDA
                     IIQSILRGQVDLETQDYQPVVMYLNGEYWGLINMRERYDENLFAGYHSYAEVGDIDLL
                     KIDADKKTYEANEGDTVRYKEMMAFVNANNLAVQANYDIMRTKYVDVDNMINYYIAQI
                     YCQNTDWPANNTRMWRPRRENGKFRFPLYDTDFGYGLWGGDAYSNNLSRVMSTTADYA
                     WSTVLFRKMMDNADFKNEFIQRYAYMINTVYSSTRLNTIANNIENTIKTERDTYNDAE
                     WTRSVNSAYNTASMISWGTTRIDQARSHINSQFGSKGWQTLTVNYTASQGSVTLCGLP
                     VTAGYSGQQYANTPIRMNAIPADGYRFVRWENGSGTSLSTDQQYYLTITAAYTIKAVF
                     EARPTVTNLKINEIMASNATVYANDYGKYNDWIEIYNAGTSAVDLAGLYISDDVANPT
                     KYKIPYGSPEKTTIPAGGYKILWANGDNYGGVLYLPFKLNKDGGIICISQKSSTGTVT
                     TIDNITYGSQITDLSYGCYPDGNANKIIFTAPTPGASNTVQSATFIDGLKITEFMAKN
                     ASTIKEETGTYADWFEIHNTNATSVDIGGLFVTNDLTNLNMYMIPKGQPSLTTIPAGG
                     YLILWADKQIKINPNHVDFKLNAEKGDIAIVQLRGASNYIIDQVSYTNQGEDISYGRF
                     PSVTSNFRYLAKPTPGAVNNNTITIPQKTGITINEILAWNTSTVQDETGSYADYIEFY
                     NAGTTAVDLGGLFVSDSAGYSLK"
BASE COUNT         2225 a          982 c         1181 g         2288 t
ORIGIN      
        1 ctaattccgg tttttgattg cgttcttcta aatatgcata aaacttcatt gcatgagcat
       61 attcttcttt tgattgaatt tgaagccaat ttgctattcc tttgtatcct ttagaattag
      121 cccaagctgc cattgaaaga tataaatatg atgaatataa ttctgcattt atttgtttat
      181 tcatgctttg tactactgtt gttttcatga tttctactga attaaattat ttcttttagt
      241 ttctccaaat atacacattt ttgaattact attattctct ttatttaatt atattcaatc
      301 attctgtcag taaagtatat gatttcctta cattcaaaga aaaaaatata catacttatg
      361 tattattttt gttatgtaat agtgtttttt gtatttagtg aaaaggaata aagcaagttg
      421 tttattgcaa attgtcaaac ctacatatga cgtatgtttt taaaaataaa caacattcaa
      481 ttggtgtatt catttgttta ttgggtgtat tattttacaa ttatatgttg tttgtatgag
      541 ttaattgtat cggtaattgt aataaaatat actccttttg ttaggttgtg taaagatatt
      601 tcaccttgtc ctttggtaca ttgaattatt tgatttgaaa caattttacc catactattt
      661 gtaattgtaa ttaaagctgt ttcggaataa gcatagggta attcaaaata acacagtgaa
      721 ttgcttgtag gattaggata gagagcaata gtttgtatac ttttttgtat agaactatta
      781 ggatttgtaa ttttgaatag tatatcataa tatgctgact ggttaaagaa atcagttgca
      841 gttactcgta tagtatatgt tcctgtatcg gcaattgtag tattagctat aaattcatat
      901 gtgtaaggat taaatgtaag ccaattgggt aacgcacttc cattgggcat acttgcagta
      961 agagtaagaa catcattgtt tgcatcgcta aattttgtgc gaggaaatac ataatgtaaa
     1021 tcataataag catcaaaact gatagtggta ggtgaagtgt tggcaatagg tggtacattt
     1081 tctgaactgc cattgttttc atatgcttca acttcaaata gcgaatatcc ccaaccggta
     1141 gcacgttttt tgaaaagaat ttgaatataa cgacatgaaa ttggttcaaa tactatattt
     1201 acctccgatt tttgaccttg accaatgttg aatactgttg tccaatcttg tttgttactt
     1261 gacacacgta tttcgtattg gtcgccatac gctgcttccc aaaataactt catgcgagaa
     1321 atagtttgct gttcaagcat atcaaattca atccattcat tattgttgta tatactagac
     1381 catctggttg ttttatcacc gtcgttacat aaaggtattc gcttgatagc atcttcttcg
     1441 ctcgaggcgg taacatatgc atataataat ttatttggtg attgaattat agaatcacga
     1501 acaactacgt tccattgtga acgagctgtt tttcctccaa tgtcggtacc ggtaattata
     1561 aatttactta atccaataga atcgggtaat acagaaattg caagaattga atcgtttaca
     1621 atagttgcta tagctggtcc atctgaatta attgtatatg atagttgttt ttcatcttca
     1681 aaaaattggt tgaggtttat atattgcgga ataggattag aatttttaaa taaaattgta
     1741 tcttttactc gtgcaatatt tttgggggca tagttgtggt taggatctcg aggaattgta
     1801 atgagattgg ggtatgtttt tttgagagaa tctaatacat atttcatgtc atttaaattt
     1861 ccgttaccgt catgaccggt ataggtccat ccccaaccac cggcataacc gttgtctatt
     1921 aaataagaca ttgcttgtgt tgttgtaagt tgagatgaag gagcaaatgg tttccccggt
     1981 tgacgtataa ttccaagagc cgcaaattca ccaattaata atggtttatc taattgccaa
     2041 tattgcacag ggttatgaaa aggagaatat ttgtcgccat aataatcagc ataataatga
     2101 agcatataaa aatcttgata tccatcggca tcacctcctt tatcaaataa ttcggcattt
     2161 gaatagtagt ttttggagct gtatatatta ggtaaagttg cagtagctat agcattccaa
     2221 ctgccatttg aaacttttgc ctgagggtcg gttctgtgta ttgctcctgc gcaccaattg
     2281 gtaaatcgtt gaatatattt catatctact ttttctataa gtggccaatt tgcaattgaa
     2341 gtcatgcctt cgggctcgtt aaaaatttcc caacacaata tagctgtatg accttttact
     2401 ctctctacca taggtataag agcatacttt atataagcca tacttgccgc agagtcttca
     2461 agtaatcgtc tgtttattcg tagtctatta tttgcatata tatctgtttc tgtatccttt
     2521 ttcttgtgca tcatatcaaa tgaccataaa caaaggctta ttacaatgcc gtgttctgca
     2581 gctatgtcaa gagctgtttc taaattatta agagcttgtt cgcttatgcc tattactgtg
     2641 tcgttagcaa atgtaggtgt gcttctgcca tcaacatgta tccaccaacg tacagaattt
     2701 ccgccggcat cagatacatt tttcataata gctgtaaatg tttcagggtt aaattgtgtt
     2761 aaatcgttac cgaattgcat ccaagcaagg ttaatacctg aaaaaaaatg ttcatgtccc
     2821 ttatgttcaa tatattgttg gctatatgca ttgcatatat atataaaaca aaacgcacaa
     2881 gtaataagaa tggatttcat agaattattg ttttaattta ttctgtaaaa ttataataaa
     2941 ttcatattat gtatagcata taatcttatc aaacaactta tattatttta tcaatttcaa
     3001 atagtaatag ttaaaattgc gtgcttatac tcaatttttt atttttataa aaaaaacata
     3061 taatttcatg ttattgaatt gttaaaattg tgtaagtttg atgttcccga attttcattt
     3121 ttttataata aaattaattg attatcaaat tataacaaat gttaattttt ttataaaaat
     3181 atatcgggtt acgtataaat taattgtcaa gaaatagtta aatatataaa aaattggggg
     3241 attttttaat agagagtgtg catatgaaga aatcgttact tgtattgctt ctactaattg
     3301 tagtttttac acaaagtttt tcgcaaatta aaattaatga agtgcaatcg tcaaacacaa
     3361 agacgattaa agattctgac tttcaagaat atgtggattg gatagaatta tataatcctg
     3421 gtacgagcag tgtaaatatt ggtgggtatt atttaaccga tgataaagat gaacctcgta
     3481 agtggcaaat acctacaggt acatctattg ctgccaaagg ctatttactt atttggtgtg
     3541 atgggaaaaa tataaataga cataccaact ataaattagg ttctgatggt gaacgtatag
     3601 tattgtattc aaatactatg ttgtttcaag attctatcaa attcccggaa atagcaacag
     3661 attttacgta tggtagaaca gtgaacggag caggtgagtg ggcattgtta acagcgccaa
     3721 caccgggagc taccaatgtt tcaactgttg taaaaggatt agcacctaaa cctaccttta
     3781 atattcaagg aggtttttac acatcaaacc aatcggttac attatctaca actattccgg
     3841 gggctgtaat tagatacact acagatggta gtgaacctac agcgtcgtcc ccaatttata
     3901 ccggagctat tacagcacaa aaaacaacaa aaacaactca aaaatatggt aataatagat
     3961 taaataaaac cggaattcaa aaatatggat ggccaagttc attaacatat ccaaccggat
     4021 attacgatgg aacacgtgag tatggttttg taattaaggc aaaagtgttt catcccgatt
     4081 atttgccaag ttatactgcc ggacaaacat attttataaa tatgcgaaaa cctacattgc
     4141 cggtggtatc tattactact gattttaata atttttttag tgcagataca ggtatttata
     4201 tacaaggaac aaacggtatt agtaatggtt atgtaacagc caattggtat caagattggg
     4261 aacgaaaagg acatattgaa tattttgatg ctaccggaag tcgaaaattt ggtgtttctg
     4321 ttggtatttc aacttcggga gcagtatcga gaagttatga tttnnnnnnn nnnatttaaa
     4381 atcgttaaac attgtaatgc gaaataaata tgaaacagga acaatggagt atcaactctt
     4441 tggttctgat ggtttaagtt cgtataaatc aattatgctt cgcaattccg gtaatgactg
     4501 ggaaatgggt aattttgcac gtgatgctat aattcaaagt attctacgcg gacaagtaga
     4561 tttagaaacg caagattatc aaccggttgt tatgtatttg aatggcgagt attgggggct
     4621 tataaatatg cgtgaacgat atgatgaaaa tttatttgcc gggtatcatt catatgctga
     4681 agttggagat attgatttat tgaagataga tgccgataaa aaaacatatg aagccaacga
     4741 aggtgatact gtgcgataca aagaaatgat ggcttttgtg aatgctaaca atttggcagt
     4801 tcaagctaat tatgacataa tgcgtacaaa atatgtagat gttgacaata tgataaatta
     4861 ttatatcgct caaatatatt gtcaaaatac cgactggcct gcaaataata cacgaatgtg
     4921 gcgtccacgc agagaaaacg gtaaatttag atttccattg tatgataccg attttggata
     4981 tggattatgg gggggagatg cttattcaaa taatctttca agagtaatgt caaccactgc
     5041 cgattatgct tggtctacag tattatttag aaaaatgatg gataatgctg attttaaaaa
     5101 tgagtttatt cagcgttatg catatatgat taatacagtt tacagttcaa cacgattaaa
     5161 tacaatagca aacaatattg agaatactat aaaaacagaa cgtgatacgt ataatgatgc
     5221 tgaatggaca cgaagtgtta attctgctta taatacagca tcaatgattt cgtggggaac
     5281 aactcgtatt gatcaagctc gttcacatat aaattcgcaa tttggcagca aagggtggca
     5341 aacattaaca gtaaattata cagcaagtca agggtctgta acattatgtg gacttcctgt
     5401 tacagcagga tattcaggac agcagtatgc aaatacacca attcgtatga atgcaattcc
     5461 tgccgatggt tatagatttg tacgttggga aaatggcagt ggaacaagcc tttcaaccga
     5521 tcaacaatat tatttgacaa ttactgcagc atatacaatt aaagctgttt ttgaagctcg
     5581 acctactgta acaaatttga aaattaatga aataatggct tcaaatgcca ctgtttatgc
     5641 aaatgattat ggtaaatata atgattggat tgaaatttat aatgcaggta ccagtgctgt
     5701 tgatttagcc ggtttatata tatcagatga tgttgcaaat cctacaaaat ataaaatacc
     5761 gtatggtagt cctgaaaaaa cgacaatacc tgccggtggg tacaaaattt tatgggcaaa
     5821 tggcgataat tatggtggcg tattatattt accattcaaa ttaaataaag atggtggtat
     5881 aatttgtatt tctcaaaaat catcaacagg aacagtaacg actattgata atattaccta
     5941 cggttcacaa attactgatt tgtcttatgg ttgttatcct gatggtaatg caaataaaat
     6001 aatttttact gctcctacgc ccggtgcttc aaatacagtt caaagtgcta cgtttattga
     6061 tgggctcaaa attaccgaat ttatggcaaa aaatgcctca actataaaag aagaaacagg
     6121 aacatatgct gattggttcg aaattcataa tacaaatgca acatctgttg atattggagg
     6181 tttgtttgtg acaaatgatt taaccaacct aaatatgtat atgattccta aaggacaacc
     6241 ttctttaact acaattccgg caggtggtta tttaatatta tgggcagata aacaaattaa
     6301 aattaatcct aatcatgtag attttaaact caatgcagag aaaggggata ttgcaattgt
     6361 acagttacga ggtgctagta attatatcat agaccaagtt tcgtacacaa atcaaggtga
     6421 agatatttca tatggacgtt ttccttcggt tacatctaat ttcagatatt tagcaaaacc
     6481 aacccccggt gctgtaaata acaatactat tacaataccg caaaaaacag gaattactat
     6541 caatgaaata ttggcttgga atacatctac tgttcaagat gaaacaggaa gttatgccga
     6601 ttatatagaa ttttataatg caggtactac agctgttgat ttaggaggat tatttgtaag
     6661 tgattcagct ggatattcgt taaaat
//
LOCUS       DAOTOW010000020         6651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_9956_length_6651_cov_3.367041, whole genome shotgun sequence.
ACCESSION   DAOTOW010000020 DAOTOW010000000
VERSION     DAOTOW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9956_length_6651_cov_3.367041"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2030)
                     /locus_tag="P5243_00675"
     CDS             complement(93..2030)
                     /locus_tag="P5243_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRS17988.1"
                     /translation="MATLNSKIPQGPLAQKWQYYKDHQKLVNPANKRKLDIIVVGSGL
                     AGGAAAATFGELGFNVKCFAYQDSPRRAHSIAAQGGINAAKNYPNDGDSVFRLFYDTI
                     KGGDYRAREANVYRLAEVSNNIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAK
                     GQTGQQLLLGAYSALSRQIGKGTVKMYNRTEMMDIVVIDGKCRGIVTRDLVTGEVEAH
                     FAHAVVLATGGYGNVFFLSTNAMGSNGSAAWKAYKRGAYFANPCYVQIHPTCIPVHGE
                     FQSKLTLMSESLRNDGRVWAPKLKEDAEAIRAGKLKPEDIPDENRDFYLERRYPAFGN
                     LVPRDVASRAAKERCDAGYGVESGEAVYLDFKYAIERLGRDTVEARYGNLFQMYEKIT
                     DQDPYKTPMMIYPAIHYTMGGIWVDYELQTTIPGLFAAGEANFSDHGANRLGASALMQ
                     GLADGYFVLPYTVQNYLADELHTPRMNPKEGEFDKTVKEVKAHIEKLMNVKGKKSVDS
                     FHKRLGKIMWDYVGMARNKEGLEKAITEIQALRKEFWSDVRIPGSPNELNVELEKANR
                     VADFLELGELMARDALHREESCGGHFREEHQTPEGEAARVDDKFAYVGAWEFTGVDSE
                     PILHKEELIFEECKLVQRNYK"
     gene            complement(2061..2756)
                     /locus_tag="P5243_00680"
     CDS             complement(2061..2756)
                     /locus_tag="P5243_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRS17989.1"
                     /translation="MANIFSYSIGRKLLMSLSGLFLVMFLLVHLLVNSFLILDPLFGT
                     AEGEMYNAGVHFMTTNPFIRIMEPILAGGFIVHIVYSIILTIQNMRARGSKSYASGNK
                     TPDVEWSSKNMFVLGIALLSFLAIHIAQFWVKMKITGDPRLDYTVEGHNAYVLVHETF
                     QITWVMICYVIGGIALAFHLSHGFWSAFHTVGLSNSIWIPRLQKISVVIAWIIGLGFS
                     TIALVQYLVYPLS"
     gene            complement(2949..5381)
                     /locus_tag="P5243_00685"
     CDS             complement(2949..5381)
                     /locus_tag="P5243_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17990.1"
                     /translation="MKKLIVCICVLCIYCITNGQNIGDYRTCLEGEYTWDNISVWQQF
                     NGTTWISALDYPGKNTSALTVTICNNASVICNKPIEEFPIQNLIIDSTSILTITSKNI
                     KIEQDLCVWGTLSIQSNIGTVICNTIHLYGTIIQDYSKSFTITSDAYINGFVLQGNGT
                     TQLTIHKNLYFQNKPCKVSQLGLYVLGKTYIQTSVEFNSIAGDKTFIDTLFITNCTVH
                     ISVGETYTCNSSVVIQNATIQTVAFPFITIRNNLILANAYCTRHDDYFGTFTVQGNIE
                     IPSNTESTIESACIELQGNCNIRGTLNITDKKGVKTIYGSFTVFTAGMFKNSGNDRIR
                     IWGNLENYGTCNNGTNGVFQLLGINKHISGTLKTPRLLIDGLYYNNNTLEIISDFGGN
                     GTLIQTNYANLSIQSPSSPTIIADAYGNIVSYTRGGNQDVRCSKFYILKAACNKNSLL
                     LQQHIESIYQIQFTKPCFINCNSYTITFESISDTTILGIDNFDRGFILMSGSIIINQI
                     PFQKSVFIPLFTSSRIDACAGIRIQELDSLNNSFTILALEKQVVDFPGVHSSNLLNSG
                     IVNTTYHIQSASTQASISLYWHSSSEMPAFNRNFCSIMHYNGTQWHALSDYNEAQQIA
                     TNIYAIQATTTAFSPFAITSNAYFLNIHTIECTVLQNSQNIVIQGNIHDSFNLEHIQI
                     YSSTNGKQFTQLAEIDVSNQQSFAYYDCTHYLSDYIYYKCVGVYNDKSIIEYTIVSIA
                     NKHNSLLTIQKTADYLLIEYPHAIPVTLYTPQLQAIAKFYTNQRFSYTAVSQGLYFLY
                     CNKTYIPIVITQ"
     gene            complement(5546..5989)
                     /locus_tag="P5243_00690"
     CDS             complement(5546..5989)
                     /locus_tag="P5243_00690"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF011116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRS17991.1"
                     /translation="MAGNITFTMIKPDAVKNGHIGSILKMITDAGFRIVAMKFTRMSA
                     AKAGKFYDIHKERPFYGELVEFMTSGPIVAAVLEKTNAVEDFRKLIGATDPAKAEPGT
                     IRKLYATSLGENAVHGADSDENALREAGFHFATSEWFDKDGNCMG"
     gene            complement(6090..>6651)
                     /gene="pgmB"
                     /locus_tag="P5243_00695"
     CDS             complement(6090..>6651)
                     /gene="pgmB"
                     /locus_tag="P5243_00695"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HRS17992.1"
                     /translation="IGISIDEHFNEQLKGVSRAVCIDLILAHGNVKKTQIEKEQLAAQ
                     KNQWFLEYIEHISPQDVFPGVIEFLTDLRANGFKMAIGSASKNAPLLLEKMQIAHYFD
                     AIVDGNTIEKAKPNPEVFLKGAQQLGIAPSACVVFEDALSGVQAAKSAGMYCIGVGSK
                     EHLTLADECISSFTEMTVERIKQIQR"
BASE COUNT         2041 a         1173 c         1106 g         2331 t
ORIGIN      
        1 ccacacttta agtgtgaggt tcaatccttt tttttcgctc ataattttcc tatattttgt
       61 ttcctttttc taagaggaag tttgtactat gattatttat aatttctttg aactaattta
      121 cattcttcaa atatcaattc ttctttatga agaataggtt ctgaatctac tcctgtaaat
      181 tcccatgcac ctacataagc aaatttgtca tcaacacgag cagcttcgcc ttcgggtgtt
      241 tggtgttctt cgcggaagtg tccaccacat gattcttcgc ggtgtaacgc atcacgtgcc
      301 ataagttcgc ctaattctaa gaaatctgct acacggtttg ctttttctaa ttctacgttc
      361 aattcatttg gtgaaccggg aatacgcacg tcagaccaaa attctttacg taatgcttgg
      421 atttcggtaa ttgctttttc caatccttct ttgttacgcg ccatacctac atagtcccac
      481 attatttttc ctaagcgttt gtggaaactg tcaaccgatt ttttaccttt aacgttcatc
      541 agtttttcaa tatgtgcttt tacttctttc actgttttgt caaattcacc ttctttcgga
      601 ttcatacgtg gtgtatgtaa ttcgtctgcc agataatttt gaacagtata tggaagaaca
      661 aaatatccgt cagccaaacc ttgcataaga gcagatgctc caagacgatt agccccgtgg
      721 tcagaaaagt ttgcttcacc agctgcaaac aagccgggga tagtagtttg taattcgtaa
      781 tcaacccaaa taccacccat agtatagtga atagcagggt atatcatcat tggagtttta
      841 tatggatctt ggtcggtaat tttttcgtac atttgaaata agttaccata gcgagcttca
      901 acagtatcac gacctaaacg ctcaatagca tatttaaagt caagatatac tgcttcgcca
      961 ctttcaacac cgtatccggc atcgcatcgt tctttagctg cgcgagaagc aacgtcgcgt
     1021 ggtactaagt taccaaatgc agggtaacga cgctctagat agaaatcgcg attttcatca
     1081 ggtatatctt ccggttttaa tttgccggca cgtatagctt ctgcatcttc ttttaatttt
     1141 ggtgcccata cacgaccatc gttacgcaac gattccgaca taagggtaag tttagattgg
     1201 aactcgccat gtacaggtat acatgttggg tgaatttgta cataacatgg atttgcaaag
     1261 tatgcaccac gtttgtaagc tttccatgcc gcactaccat tagaacccat tgcattggtt
     1321 gataagaaaa atacattacc atatccaccg gttgcaagta ctactgcatg tgcaaaatga
     1381 gcttcaactt ctcctgttac taaatcgcgt gttactatac ctctacattt tccgtcaatt
     1441 acaactatat ccatcatttc ggtacggttg tacattttaa cggttccttt accaatttga
     1501 cgacttaatg cactgtatgc tccgagtaat aattgttgtc cggtttgacc ttttgcataa
     1561 aatgtacgcg atacttgtgc tccaccaaac gaacggttgt ctaataaacc tccatattcg
     1621 cgagcaaaag gaacaccttg tgctacacat tggtcaatta tattattgct tacttctgcc
     1681 aaacgatata cgtttgcttc gcgagcacga tagtcaccac cctttattgt atcgtaaaat
     1741 aagcgaaata cgctgtcacc atcgtttgga tagttttttg cagcatttat accaccttga
     1801 gctgcaatag agtgtgcacg acgtgggctg tcttgatatg caaaacattt tacattgaaa
     1861 cctagttcac caaaagttgc agcagctgca ccaccagcca accctgaacc aacaacaatt
     1921 atatcgagtt tacgtttgtt agcagggttt actaattttt gatgatcttt ataatattgc
     1981 catttttgtg ctaatggacc ttgaggaatt ttactgttta acgttgccat agtgttttaa
     2041 tgtttttatg gtaaatacaa ttatgataat ggatacacaa ggtattgcac taaggctata
     2101 gtactaaatc ctaagcctat aatccatgca attaccacac ttattttttg taagcgaggt
     2161 atccaaatag agttgcttaa tcctactgtg tggaaagccg accagaatcc gtgtgataag
     2221 tgaaatgcta atgctatacc acctattaca tagcatatca taacccatgt gatttgaaat
     2281 gtttcgtgta caagcacata agcgttgtgc ccttctacgg tatagtctaa acgtgggtcg
     2341 ccggtaattt tcatttttac ccaaaattga gcaatatgaa ttgccaaaaa tgacaataat
     2401 gcaataccca atacaaacat gttttttgac gaccactcta catcaggtgt tttgttaccc
     2461 gatgcgtagc ttttagagcc gcgtgcacgc atattttgaa tggtaagaat aatagagtac
     2521 actatatgta ctataaatcc acctgccaat ataggttcca taattctgat aaatggatta
     2581 gtagtcataa agtgaactcc ggcattatac atctcgcctt cggcagtccc aaaaagcggg
     2641 tcgagtataa gaaatgaatt taccagtaag tgaaccaata aaaacattac aagaaatagt
     2701 cccgataaac tcatcaggag ttttcttcca attgaatagc taaaaatatt cgccatatta
     2761 aataatagtt ttatgaattt tgaaatttct tttagttgca acaaaattac agaaaatatt
     2821 ctaatgccat agtaaataag tgataaatct cacaaaaaaa actatttcat attttttatc
     2881 ataccttatc aagtagttaa aaaacgttac ttgtaaaaaa atataaaatt gttatgattg
     2941 ccttatactt attgtgtaat tactattgga atatatgttt tattacaata tagaaaatat
     3001 aagccttgtg aaacggctgt atatgaaaac ctttgattag tataaaattt agcgattgct
     3061 tgtaattggg gcgtataaag agttacaggt atggcatgtg ggtattctat caataaataa
     3121 tcagcggttt tttgaatagt aagaagtgaa ttgtgtttat ttgcaatact tactattgta
     3181 tattcaatta tactcttatc attgtaaaca cctacgcatt tataatagat ataatctgat
     3241 aagtaatgtg tgcagtcata atatgcaaat gattgttgat ttgaaacatc tatttctgcg
     3301 agttgtgtaa attgttttcc attggttgaa gaatagattt gtatatgttc taaattgaag
     3361 ctatcatgta tatttccttg tataacaatg ttttgagagt tttgcaaaac agtacattct
     3421 atagtgtgaa tatttaaaaa atatgcattg cttgtaattg caaaaggcga aaatgccgtt
     3481 gtagtagctt gtattgcata aatattagtt gcaatttgtt gagcttcatt gtaatctgag
     3541 agtgcatgcc attgcgtgcc attgtagtgc attatactgc aaaaatttct attgaatgcc
     3601 ggcatttcgc tgctgctgtg ccaatataga cttatagatg cttgtgtgct tgcagattgt
     3661 atatgataag tagtattcac tatcccactg ttcaataaat tacttgaatg taccccagga
     3721 aaatctacta cttgtttttc taaagcaaga atggtaaatg aattatttag actatcgagt
     3781 tcttgtatgc gtattcccgc acaagcatca atacgagatg atgtaaataa tggaataaaa
     3841 acgctttttt gaaatggtat ttggtttata attatacttc ccgacataag aataaagcct
     3901 ctgtcaaagt tgtcaattcc taaaattgta gtatcactta ttgattcaaa ggttatagtg
     3961 taactgttgc agtttataaa acatggtttt gtaaattgta tttggtatat tgattctata
     4021 tgttgttgca gaagtaagga atttttattg cacgcagctt tgagtatata aaatttactg
     4081 catcgtacat cttgatttcc tccgcgtgtg tagctaacga tattgccata agcatcagct
     4141 ataattgttg gtgatgaagg gctttgtata ctaaggtttg cataattagt ttgtataagc
     4201 gtaccgtttc ctccaaaatc tgatataatt tctaaagtat tgttattata gtataatccg
     4261 tcgattagta aacgtggtgt ttttagagta cctgatatat gtttatttat gccaagtaat
     4321 tgaaatacac catttgttcc attattgcat gttccataat tttctaaatt tccccaaata
     4381 cgaattctat cgttgccgga gtttttaaac atgccggcag taaatactgt aaatgaacca
     4441 tatatagttt taacaccttt tttgtcggta atattgagtg tgcctcgtat attacaattc
     4501 ccttgtaatt caatacatgc actttctatt gtggattcgg tatttgaagg gatttcaata
     4561 ttcccttgaa cagtaaatgt gccaaaatag tcgtcgtgcc ttgtacaata agcattggca
     4621 agtattaaat tgttacgtat tgttatgaaa ggaaaggcta ctgtttgtat ggtagcattc
     4681 tgtattacaa ccgaagagtt gcaggtatat gtttcgccta ctgatatgtg tactgtgcaa
     4741 tttgtgataa ataaagtatc tataaatgtt ttatctcccg caatggaatt aaattctact
     4801 gaagtttgaa tataggtttt tcctaacaca tataatccta attgacttac tttacaaggt
     4861 ttgttttgaa agtatagatt tttatgaata gtaagttgtg tagttccgtt gccttgtaac
     4921 acaaatccgt taatatacgc atcagaagtt atagtaaatg atttggaata atcttgtata
     4981 atagtgccat acaggtgtat agtattacat attacagtac ctatattact ttgtatagaa
     5041 agagttcccc atacacataa atcttgttca attttgatat ttttagatgt aatagtaagt
     5101 atgcttgtag aatcaattat aagattttgt ataggaaatt cttctattgg tttgttacaa
     5161 atgaccgatg cattgttgca aattgttaca gtaagtgccg aagtgttttt tcctggataa
     5221 tcaagtgcag aaatccacgt tgtgccgtta aattgttgcc aaacggaaat attgtcccat
     5281 gtatattcac cttctagaca agttctgtaa tcgcctatgt tttgcccatt tgtaatacaa
     5341 tatatacata gtacacagat gcatacaatt aattttttca taattttttt atgttgcggt
     5401 gtacaattat gtggtaattg tacagattaa tatagtttca aaaatatgaa aaaaataata
     5461 aaaaatacat tgaatccaaa ataaataaaa aaaggctgta gttacataac cacagccttg
     5521 caatattttt caataaagga attaattacc ccatacaatt tccgtcttta tcaaaccatt
     5581 cgctggtagc aaaatgaaaa cctgcttcac gaagagcatt ttcatcacta tcggcaccat
     5641 gaacggcatt ttcacctaat gaagtagcgt ataatttacg tatagttccg ggttcagcct
     5701 tagcaggatc tgtagcacct ataagtttac gaaaatcttc tactgcgttt gttttttcta
     5761 aaactgctgc tactattggt cccgaagtca taaattcaac taattcacca tagaaaggac
     5821 gttctttatg aatatcataa aatttacctg ctttagctgc actcattcga gtaaatttca
     5881 ttgctacaat tctaaaacct gcatcggtta tcattttgag tattgaacct atgtgtccat
     5941 ttttaaccgc atcgggttta atcattgtaa aggtaatatt tcctgccata aatctatatt
     6001 tttatattaa ttaaagaata gggctaattt acattttttt ttgtaattga taattatttg
     6061 tatcagtata ttttttaata aaatcgcaat tacctttgta tttgtttaat acgttccacc
     6121 gtcatttcgg taaatgagct aatacattca tctgccaaag taagatgttc tttacttcct
     6181 acgccaatac aatacattcc tgcagatttt gccgcttgca ctccgcttaa tgcatcttca
     6241 aaaacaacac atgcagaagg cgctatgcct aattgttgtg cacctttcaa aaaaacttca
     6301 ggattaggtt tggctttttc aattgtatta ccgtctacta ttgcatcaaa atagtgagct
     6361 atttgcattt tttctaataa aagaggagca ttcttactgg cagaccctat agccatttta
     6421 aatccattgg ctcgtaaatc ggttaaaaat tcaatgactc ccggaaaaac atcttgtgga
     6481 cttatatgtt ctatatattc caaaaaccat tgattttttt gagcagcaag ttgttctttt
     6541 tctatttgtg ttttctttac attaccgtgt gctaaaatca aatcaataca aacggcacga
     6601 ctaacacctt ttaattgttc attaaaatgc tcgtcaatag aaattcctat a
//
LOCUS       DAOTOW010000021         6404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_10431_length_6404_cov_3.047252, whole genome shotgun sequence.
ACCESSION   DAOTOW010000021 DAOTOW010000000
VERSION     DAOTOW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10431_length_6404_cov_3.047252"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4327
                     /locus_tag="P5243_00700"
     CDS             <1..4327
                     /locus_tag="P5243_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS17993.1"
                     /translation="APGTAVITATSVSAPTISGTATITVSSILPTAIAITPTSAVSVN
                     VGSTTNLSADVIPTNATDKSVTWSSSNTSIATVSATGVVTGIAPGTVTITATSVAAPT
                     ITATKSVTVLAILPSSITVQLDKTSIPVESTAIATATVLPANAGDKSVTWSSSNTAVA
                     TVTPNGVVTAIAPGTATITATSVVAPTISGSATITVTAILPTLISVQAPTSMTVGNTA
                     TATATVTPANTTNKNVTWSSSNTAVATVSASGVITAVSAGTVTITATSVSAPTVSGSA
                     SIVISNNVVVPTAITITPTTAVTVLPGQTTVLGLTFTPANTTEQGVTWVSSNPAIATV
                     NPNGVVTGVAPGTVTITATSTASGSVSATKQVIVSPILPTAITILPTTAVSVSVGATT
                     TLTVDVTPANATDKSVTWTTSNSAIATVSESGVVTGVAAGTVIITATSVAAPTVSATK
                     QVQVNAVLPSGITVTLTPNSLSVGGTSTAIATVSPANAGDKSVTWSSSNTAVATVNAN
                     GLVTAVAAGTAVITATSVAAPNVSGSATLNVSSVLPTSIVVNLTSSTISVGATTTATA
                     TISPANAGDKSVTWSSSNTAVATVNSNGVITAVGPGTAIITATSVAASNITGNATITV
                     NTVLPTGILVTLSPNSLSIGGTSQASAEVSPANAGNKNVTWSSSNTSIATVNATTGVI
                     TAISAGTVLITATSVAAPGVSGTASLQVSSILPSSIVVTLNPASITIGSTSTATAEVN
                     PANAGNKSVTWTSSNTAVASVNANGVVTAIAPGTATITATSVAAPTVSGGATITVSNV
                     PVTNIVVSPASVIIEIGNTQAIGVTIEPSNATNKNVTWASTNTAVATVNPNGVITAVT
                     KGTATITVTSVSNSNVSKAIVVECVSKSALLSAINNAQAIYNSAVEGSADGQYPVGSK
                     ATLQSAISAANAVYTNPSSTQAQIDQAQSTLQAAVTTFQNSVIGVNKTELSNLITQAQ
                     TAYNLAVEGTAAGQYPIGSKATLQTAINSAIQVNNNPLASQADVNSAKAALQTAYDTF
                     INSQIVVGPVDKTQLQASITQAQTLYTNSVEGLAEGQYPSGSKNILLNAISTAQAVNN
                     NASATQTQVNTANTNLQTAIAEFQAKVIHVDKSALVTQIATSQAIYNAAVEGTLNGQY
                     PVGSKANLQQAITVAQGVNTNVLATQTQVNQAVTALQNATNTFLSLQINIVLDKAPLQ
                     TKITQATTSLAKADNNVGTAPGQYPQAAVNELIAAKAAAENILATATTQAQLNTAVTN
                     LNTAIQAFENSVVPVVVDKSQLAELIAEADELFETTVIGTKPGEYGQLEYFNLYNATN
                     SARVAYNDNTKTQAQIDAQVVLLQAAINSYKNSKVPTSIDDIVIVSVKAWPNPCTTSL
                     TVSASTEIAAITLTNIIGAQQVVSKSIDGESASIDVVSLANGIYFVTVTLQDGSSKIV
                     RIVKQ"
     regulatory      4454..4552
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4612..>6404
                     /locus_tag="P5243_00705"
     CDS             4612..>6404
                     /locus_tag="P5243_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS17994.1"
                     /translation="MKKWNVVVCAVLFTISAYGQKWNLTGVVSNPQGERLAGASVYIE
                     NSFIGTYSNSNGEYSLEKLQAGTYTVVCSYMGYTNVIKQILVQSNTVYDIVLSQKAIT
                     IEPVVLTAIKAKEQTPVAKINLSKQEIEQQYGTKDIPYVLEQTPSIVATSDNGTGMGY
                     TGMRIRGTDMSRINITVNGIPLNDAESQTVFWVNMADFASSVEEVQIQRGVGTSTNGA
                     ASFGASVNFQTVDLQEKPHAQISSSVGSFGTFKQTMSAGTGILDSCLYFGIRVSRLDS
                     DGWVERSFSQHSAAHLTGSYLKGSHRLTANVLLGKERTGISWEGLPDYMLSTNRRYNV
                     TGLYYDDFGNEAFYDNETDNYWQNHYSLSYGGILSKHITFSLTTHATTGKGFYEQYKD
                     NAKFSKYGIPVVLLSDTVVVINQRQYIFPDSTIKRSDLIRQKWLDNIFYGYTSGITYT
                     KNNTEISLGNSYNIYKGNHFGKVIWVQMIPDFDKSYTWYDNDSEKRDVMVFVKVQQKL
                     FSQLSIFVDMQYRNILYTMVGIDDDFTPLHNNFSWNFVNPKVGLYYMPNKRNKVFISY
                     AVANREPTRTDLKDAGKSAKQVTYETLYDTE"
BASE COUNT         2188 a         1130 c         1190 g         1896 t
ORIGIN      
        1 agcaccggga acagctgtaa taacagcaac gagcgtatcg gctccaacaa tttccggaac
       61 tgcaactata actgtttcat caattttacc aactgctata gcaattactc caacttctgc
      121 agtttctgta aatgttggtt ctacaacaaa tttatctgct gatgttattc ctacaaatgc
      181 aactgataaa tcggtaacat ggagcagttc aaatacatct atagcaacag taagtgcaac
      241 tggtgtagta acaggtatag ctccaggaac tgtgactatt acagcaacga gtgtagcagc
      301 tccaacaata actgctacaa aatctgtaac tgtattagct attcttcctt catcaataac
      361 tgttcaatta gataaaacat caatacctgt tgaatctact gcaattgcaa cagctactgt
      421 attacctgca aatgccggtg ataaatccgt aacatggagc agttcaaata ctgcagttgc
      481 aacagtgact ccaaatggag tagttactgc aatagctccg ggaacggcta ctataacagc
      541 aacaagtgtt gttgctccaa ctataagtgg ttctgctact attacagtaa cggctatatt
      601 acctacatta ataagtgtac aagctccaac tagtatgaca gttggaaaca ccgctacagc
      661 aacagcaaca gtgactcctg caaatactac aaataaaaat gtaacatgga gtagttcaaa
      721 tactgcagta gcaacagtaa gtgcgagtgg tgtaattaca gcggtttctg ctggtacggt
      781 aactattacg gcaactagtg ttagtgcacc tacagtaagc ggaagtgcat ctattgtaat
      841 tagcaataat gttgttgttc caacagcaat aactataaca cctactactg cagtaactgt
      901 tttaccgggt caaactacag ttttaggatt aacatttact ccggcaaata ctacagaaca
      961 aggagttaca tgggtaagtt caaatcctgc aattgcaact gttaatccta atggtgttgt
     1021 aaccggtgta gcaccgggta cagtaacaat tactgctact agtacagctt caggttcggt
     1081 aagtgcaaca aaacaagtta tagttagtcc gattttacca acggctatta ctatattacc
     1141 aactactgca gtgtcagtaa gtgttggtgc aactactaca ctaactgtag atgtaactcc
     1201 tgctaatgca actgataaat cggttacatg gactacttct aattcagcta ttgcaaccgt
     1261 aagtgaaagt ggtgttgtaa ccggtgtagc agcaggaaca gttattataa cagcaacaag
     1321 tgtagctgca ccaacagtaa gtgcaactaa acaagttcaa gtaaatgctg tattaccttc
     1381 aggtattaca gttacattga ctccaaattc attatctgtt ggaggtacat ctacagcaat
     1441 tgcaacggtt agtcctgcta atgccggcga taaatctgta acatggagta gttctaatac
     1501 agcggtagct acagtaaatg ctaatggtct tgtaacggct gtagctgcgg gtactgcagt
     1561 gattacagca acaagtgtag cagctccaaa tgtttcagga tcggctacgt taaatgtatc
     1621 ttcggtatta ccaacatcta ttgttgttaa tctaacatcg tcaacaatct ctgtaggagc
     1681 tactacaact gctactgcta caatatctcc tgcaaatgcc ggtgataaat cagtaacatg
     1741 gagtagttct aatactgcag ttgcaacagt taattctaat ggtgttataa cggctgtagg
     1801 tccgggaaca gcaattataa cagcaacaag tgtagctgct tctaatataa ccggaaatgc
     1861 aactataact gtaaatacag tattacctac aggtatttta gtaacattga gtcctaattc
     1921 attgtcaatt ggaggtacat cacaagcaag tgctgaagtt tcacctgcaa atgcaggaaa
     1981 taaaaatgta acatggagta gttcaaatac atctatagca actgtaaatg caactacagg
     2041 agttattact gctataagtg caggtacagt tttaattaca gcaacaagtg tagctgctcc
     2101 gggtgttagt ggaactgcaa gtttacaagt atcatctatt cttccgtcaa gtatagtagt
     2161 aacacttaac cctgcatcta ttacaatagg tagtacatca acagcaactg ctgaagtaaa
     2221 tcctgcaaat gccggaaata aatcagtaac atggacaagt tctaatactg cagtagcatc
     2281 ggtaaatgct aatggtgtgg taactgctat agctccggga actgctacta taacagcaac
     2341 tagtgttgct gcacctacag taagtggtgg agcaactatc acagtaagta atgtacctgt
     2401 aactaatatt gtggtatcac ctgcatctgt aattattgaa ataggaaaca ctcaagcaat
     2461 tggtgttaca atagaaccat caaatgcaac aaataaaaat gttacttggg ctagtactaa
     2521 tactgctgta gcaacagtta atcctaatgg tgtgattaca gctgtaacaa aaggaacagc
     2581 aactattact gtaacttcgg taagtaattc aaacgtaagt aaagctattg tagtagaatg
     2641 tgtaagtaaa tcagcattgt tatcggcaat aaataatgca caagctattt ataactctgc
     2701 tgttgaagga agtgccgacg gtcaatatcc tgtaggttct aaagctacat tacaatctgc
     2761 tataagtgct gcaaatgctg tgtatactaa tcctagttct actcaagctc aaatagatca
     2821 ggctcaaagt actttgcaag ctgcagttac tacgttccaa aattctgtaa ttggagtaaa
     2881 caaaaccgag ttatctaatt taataactca agcacaaaca gcatataatc ttgctgttga
     2941 aggaactgct gcaggtcaat atcctattgg ttctaaagct actttgcaaa cagcaattaa
     3001 ttcggcaatt caagtaaata ataatccatt ggcaagtcaa gctgatgtaa attctgcaaa
     3061 agcagctctt caaacagctt acgatacatt tataaattca caaattgttg ttgggcctgt
     3121 tgataaaact caattacaag cttcaataac acaagctcaa acattatata caaattctgt
     3181 tgagggctta gcagaaggtc agtatccaag tggatcaaaa aatattttgt tgaatgcaat
     3241 ttctactgcc caagcggtga ataacaatgc ttcggctact caaacgcaag tgaatactgc
     3301 aaatactaat ttgcaaactg caattgcaga gttccaagca aaagttattc atgttgataa
     3361 atccgcatta gttactcaaa tagcaacttc tcaagctata tacaatgctg ctgtagaagg
     3421 tactttaaat ggtcaatatc ctgtaggttc taaagctaat ttacaacaag ctattactgt
     3481 tgctcaaggt gtaaatacta atgtacttgc aacacaaaca caagtaaacc aagctgtaac
     3541 agctcttcaa aatgctacaa atacattctt gtcacttcaa ataaatatag ttcttgataa
     3601 agctccgtta caaactaaaa ttactcaagc aactacttcg ttggctaaag ctgataataa
     3661 tgtaggtact gcaccgggac aatatcctca agcagcagta aatgaattga tagctgctaa
     3721 agctgctgca gaaaatatac ttgctactgc aactactcaa gctcagttga atactgcggt
     3781 aactaatctt aacacagcaa ttcaagcttt tgaaaattct gttgtacctg tagtggttga
     3841 taaatctcaa ttagcagaat taattgctga agcagatgaa ttgttcgaaa ctacagtaat
     3901 tggtactaaa cccggagaat atggacagtt agaatatttt aatttatata atgctaccaa
     3961 ttcggctcgt gttgcatata acgataatac taaaactcaa gctcaaatag atgcacaagt
     4021 tgtattatta caagcagcta taaattcgta taaaaattct aaagtaccaa catctattga
     4081 tgatatagta attgtatctg tgaaagcatg gcctaaccca tgtactactt cacttacagt
     4141 gagtgcttca acagaaattg ctgctattac tcttacaaat atcattggtg cacaacaagt
     4201 ggtttctaaa tcaatagacg gcgaatctgc aagtatcgat gtagtatcat tagcaaatgg
     4261 aatttatttt gtaaccgtaa cattgcaaga tggttcaagt aaaattgttc gtatagtgaa
     4321 acagtaaatt ctacacaggc tttgcctgta tcttatatgc acgcaaaaat gcctctgatt
     4381 gcaagtcagg ggcattttta tttatatata gtttatttgt ttttgtaatt ttttatacat
     4441 ttgtacagtc aaaaataacg aataggggtg ccgtgatgta aaacatggct gagataagac
     4501 cctcgaacct gaataggata atacctgcgg aggaaattcg gtgaaatgtc taaacgtgca
     4561 tactcaagca cagcattcct attcatataa tttttcttaa aatgtagaaa tatgaaaaaa
     4621 tggaatgttg tagtatgtgc tgtattgttt acaataagtg catatggtca aaaatggaat
     4681 ctaaccggtg tagtttcaaa tcctcaggga gaacgtcttg cgggcgcttc agtgtatatt
     4741 gaaaattctt ttattggaac ttattccaat tcaaatggcg agtatagttt agaaaaatta
     4801 caggcgggaa catatactgt tgtttgtagt tatatgggtt atacaaacgt aataaaacag
     4861 attcttgtac aatcaaatac ggtgtatgat atagtattaa gtcaaaaagc aataacaata
     4921 gaaccggtgg tattaactgc tattaaagcc aaagaacaaa ccccggttgc taaaataaac
     4981 ctttctaaac aagaaattga gcaacagtat ggtacaaaag atatacctta tgtgttggaa
     5041 caaaccccaa gtatagttgc aacatcagac aatggtacgg gaatgggata taccggtatg
     5101 cgtattagag gtaccgatat gtcgcgtatt aatattactg taaatggtat accattaaat
     5161 gatgcagagt cacaaactgt tttttgggtt aatatggctg attttgcatc gtcagtagag
     5221 gaagtgcaaa tacaaagggg agtggggaca tcaacaaatg gtgctgcctc atttggagca
     5281 tcggttaatt ttcaaactgt tgatctacaa gaaaaaccac atgctcaaat ttcatcttca
     5341 gtaggatcat ttggtacttt taaacaaaca atgtctgcag gtaccggtat attagacagt
     5401 tgtttgtatt ttggtattag agtaagtagg ctcgattctg atggatgggt agaacgaagt
     5461 ttttcacaac attcggcagc acatcttaca ggctcatatt taaaaggctc tcatcgtttg
     5521 acagcaaatg ttcttctcgg taaagaacga acagggattt catgggaagg actaccggat
     5581 tacatgcttt ctacaaaccg cagatataat gttacaggtt tgtattatga tgatttcgga
     5641 aatgaagctt tttatgataa tgaaactgat aattattggc aaaatcatta ttcgctttca
     5701 tatggaggta ttttgagtaa acatataacc ttttcgttaa caacacatgc aactaccggt
     5761 aaaggatttt acgagcaata taaggataat gcaaaatttt caaaatatgg tattcctgtt
     5821 gtcttgttgt ctgatacagt tgttgttata aatcaaaggc agtatatttt ccccgatagt
     5881 actataaaac ggagcgattt aatccgtcaa aaatggttag ataatatatt ttacggatat
     5941 acttcaggta tcacttatac aaaaaataat accgaaatct cattgggaaa ttcatataat
     6001 atatacaaag gaaatcattt tggaaaagta atatgggtgc agatgattcc cgattttgat
     6061 aaatcgtata catggtatga taatgacagt gaaaaacgtg atgttatggt ttttgttaag
     6121 gttcaacaaa aattgttttc acaattatct atttttgtcg atatgcaata tcgcaacata
     6181 ttatatacca tggtgggaat agatgatgat tttacaccgt tacacaataa tttttcatgg
     6241 aattttgtaa atcctaaagt agggttgtat tatatgccaa ataaacggaa taaagtgttt
     6301 atatcatatg ccgtagctaa tcgtgaacca acacgtaccg atttaaaaga tgcaggaaaa
     6361 tctgcaaaac aggtaacata tgaaacctta tatgatacag aatt
//
LOCUS       DAOTOW010000022         6361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_10521_length_6361_cov_2.988265, whole genome shotgun sequence.
ACCESSION   DAOTOW010000022 DAOTOW010000000
VERSION     DAOTOW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10521_length_6361_cov_2.988265"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1816)
                     /gene="sppA"
                     /locus_tag="P5243_00710"
     CDS             complement(35..1816)
                     /gene="sppA"
                     /locus_tag="P5243_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRS17995.1"
                     /translation="MKRFFKYLFVTASGAFLGLALFILIGVAIITSIIAASSSSTIDI
                     KEKSVLLIRLNQPIEELSESNPLGSISPMNSDGGTIGLHDILFAIKQAQNDSNIKGIV
                     LYMDMIQAGYSTILEIRKALESFKNTGKFIYAYSDVYTQKSYLLASVATKVYINPQGM
                     FELTGISSQFVSFKDLLQKIGIQPVVFKAGKYKSYVEVFTQNTMSKENREQIQSLVSN
                     IWNTYTSQISQTRTHISKPLSTIADSILVWNAESSYTLGLTDSLVYYNDFIEIVKQSI
                     ALPSKDDISFIALESYISHCNEIQKQKKSESKATIALIVGSGTIQPGKGDYSNIYSES
                     FCKLIRDARKDSTVKAIVLRINSGGGSALASESIWHEVSLAAQTKPTIVSMGDVAASG
                     GYYIACPAHVIVAEPTTITGSIGVFGMLFNTQELFKKIGVSIDTVKSNSYADLGSPAR
                     PMSNYETAVISKNIEQTYTTFKQRVAKGRKLSLSQVEEIAQGRVWCGKDALRIGLVDT
                     LGGVQTAIAIANTKAKLNGTYTLKLYPEEKSTLEILIDKMTGNQSLLQEIETLLGKEI
                     GTIAKTIEYIPQQKGVYMQLPYILKFD"
     gene            1933..2421
                     /gene="folK"
                     /locus_tag="P5243_00715"
     CDS             1933..2421
                     /gene="folK"
                     /locus_tag="P5243_00715"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRS17996.1"
                     /translation="MNHVWLLLGANMGNEIKLFEQACKQIENKIGVCVNKSHIYTSPP
                     WGFEHTNVFYNQALHITTLLSADEVLHKCLLIEYVLGRTRNQTYGYEARIIDIDILYF
                     NDDIIQTSELNIPHKLLHKRKFTLVPLCEIASDYIHPILQLTNQKLLAMCEDEAEITR
                     CK"
     gene            complement(2433..3767)
                     /gene="nhaD"
                     /locus_tag="P5243_00720"
     CDS             complement(2433..3767)
                     /gene="nhaD"
                     /locus_tag="P5243_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HRS17997.1"
                     /translation="MFYIMILIFVLGYTAIALEHTIKIDKAASALFIGVMLWTLFMLD
                     SVNILNLGFSPGWIETEGKLDITHFIVEELEHHLIEIAEILFFLLGAMTIVEVVDQHE
                     GFKIITDRINAKKKSSLLWILGIITFFMSAALDNLTTTIVMIALLRKLIEDKKTRWIF
                     ASIIVIAANAGGAWSPIGDVTTIMLWIGGQITAMAIIKGVLLPSIMNLVVPLGILSFI
                     LQGNVEKPAKNDDEKEFTSARHRKIIFCIGIGALLFVPIFKTYTHLPPYMGMLLGLSI
                     LWMTTEIMLAKKAPEFKKKLTVYNVLKRIDTPTIFFFMGILSAVAALQSAGHLAQLGG
                     WLDEITNKNVYVINIVIGILSSIIDNVPLVAGSMGMYPIQSAGAFAVDGTFWEFLAYC
                     AGTGGSILIIGSAAGVAAMGLEKIDFMWYLKKISLLALIGYLAGCGTYILLN"
     gene            4244..6028
                     /locus_tag="P5243_00725"
     CDS             4244..6028
                     /locus_tag="P5243_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRS17998.1"
                     /translation="MKVSKYTFLVKFIRWRMQHVTDKQFVYFLSIIIGLIVGIAAVLL
                     KLLVHAIRHLVHIWSPFHEGNFLYFFLPMVGIAAAVLFMKYVIKHNVKHGVPSVLYAI
                     SMEQGKLKPHQTFSSIVTSALTVGFGGSVGLEGPSIVTGAAIGSNVGRILRLKYKHII
                     LLIGCGSAGVMAAIFNAPVAALVFALEVIVLNLAMSSIVPLLLASSAATLTSYFFLEQ
                     DVLFHAVELFNKGYELLDIPSFIVLGIFTGLVSVYFKRMYIAIESKFEHIERRKHKLY
                     IGGGLLGLLLFFFPSLYGEGYDFINMAFRGDYSFLFANSIFIIFEKSFLLTCVLFCAV
                     IFIKVIASSLTFGAGGVGGIFAPSLFIGANIGLFFWYVFEHFQIDAPVTIFVLLGMCG
                     TIAGVLHAPLTGIFLIADITSGYSLFVPLMIVSAVSFATVRIFENNSVYTYLLAKRKQ
                     LITHNKDQSVLTLLQIKKLIETNFSTVHVNATLGDLIEVIKYSKRNIFPVVDDDGMLH
                     GIVKLDDIRHIMFSPEKYNKIDVRTVMHMPKYYFSPTDDMDDVVAVIQKSGHYNFPIL
                     DNGKYVGFISRATVFSEYRKISEFFSEE"
     gene            6174..>6361
                     /locus_tag="P5243_00730"
     CDS             6174..>6361
                     /locus_tag="P5243_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS17999.1"
                     /translation="MKPTKTIPHSMRKFLATTYLVLLSALLVPVFSQVDFEFWFAAPY
                     ASQQHNPNALGPGGRPIY"
BASE COUNT         2011 a          968 c         1086 g         2296 t
ORIGIN      
        1 tttggaatgt atgtatcatg tttttttgaa tatattaatc aaattttaga atatatggta
       61 attgcatata tacacccttt tgttgcggaa tatattctat agttttagca attgtgccaa
      121 tttcttttcc caacaaagtt tcaatttctt gtaataatga ttgatttcct gtcattttat
      181 caataagtat ttctaatgtt gatttttctt cgggatatag tttaagagta taggtgccat
      241 tgagtttagc tttggtatta gcaattgcaa ttgcagtttg tacgccacct aaggtgtcta
      301 ctaatcctat acgtaaagca tctttaccac accatactcg tccttgagca atttcttcaa
      361 cttgagaaag tgaaagtttt cttcctttgg caacgcgttg tttaaatgtg gtatacgttt
      421 gttctatgtt tttagaaatt acagctgttt cataattact cataggacga gcaggtgatc
      481 ctaaatctgc ataagaattt gattttacgg tatctattga aacgccaatt tttttgaaaa
      541 gttcttgagt attaaataac ataccaaata cacctataga acctgtaatt gtggtaggtt
      601 cggcaactat tacatgtgcc ggacaggcta tataatatcc tcccgaagca gcaacgtcac
      661 ccattgaaac tatggttggt tttgtttgtg ctgccagcga aacctcatgc catattgatt
      721 cagatgctaa tgcagaaccg cctccggaat ttattctaag tacaatagct tttaccgtac
      781 tgtcttttct tgcatcacgt ataagtttgc aaaacgattc actgtaaata ttgctataat
      841 caccttttcc cggttgaata gtgcccgaac ctacgataag tgctatagtt gctttacttt
      901 cagatttttt ttgtttttgt atttcattgc agtgagatat atatgattct aatgcaataa
      961 atgaaatatc atctttactt ggtaatgcaa tagattgttt tacaatttct ataaaatcat
     1021 tgtaatatac caatgagtcg gtaagcccta atgtatatga actttcagca ttccatacaa
     1081 gtatagaatc tgctattgtt gaaagaggtt tagaaatatg tgtgcgtgtt tgtgaaattt
     1141 gagaagtata tgtattccat atgtttgaaa caagtgattg tatttgttct ctattttctt
     1201 tactcatagt attttgcgta aatacttcta cataactttt gtatttacct gctttgaata
     1261 caaccggttg aattccaatt ttttgtagta aatctttgaa tgaaacaaat tgtgaggata
     1321 taccggtaag ctcgaacatt ccttgcggat taatatatac tttggtagct accgatgcaa
     1381 gtaaatatga tttttgagta taaacatcgc tatatgcata aataaacttg cccgtattct
     1441 tgaatgattc taatgctttt cgaatttcta atatagtgct gtagcctgct tggatcatat
     1501 ccatataaag aacaattcct ttaatgtttg aatcgttttg tgcttgtttg attgcaaata
     1561 aaatatcatg aagccctatt gttccaccat cgctattcat aggtgatata cttcccaatg
     1621 gattagattc tgataattct tcaataggtt ggtttaggcg aatgagtaaa acactttttt
     1681 cttttatatc aattgtagat gatgaacttg ctgctattat acttgttatt atagcaactc
     1741 caataagtat aaacaacgct aagcctaaaa aagctcctga tgcggttaca aataaatatt
     1801 taaaaaatcg tttcatagac tacaaaattt ttacttttgt gcaaagaata tatacaaatg
     1861 taattgtttg tttttaaaat tatattagtt cagtgttttt tttaaatttc aatcagtgta
     1921 aaatgcgatg atatgaatca tgtatggtta ttgcttggtg caaatatggg aaatgagata
     1981 aaactatttg aacaagcatg taaacaaatt gaaaataaaa taggagtatg tgtcaataaa
     2041 tcacacatat atacaagtcc gccatggggg tttgagcata ccaatgtgtt ttacaatcaa
     2101 gcattgcata ttacaacttt attatcggct gatgaggtat tacacaagtg tttattaata
     2161 gaatatgtgt tgggaagaac acggaatcaa acatatgggt atgaagctcg aataattgat
     2221 attgatatat tgtattttaa tgatgatata atacagactt cagaattaaa tattcctcat
     2281 aaattattgc ataagcgaaa atttacactt gtgccattat gcgaaatagc gtctgattat
     2341 atacacccaa tattacaatt aacgaatcaa aaattacttg caatgtgtga agatgaagca
     2401 gaaataacac ggtgcaagta atataaatta atttaattta atagaatata ggttccgcag
     2461 cctgctaaat atccaattag agctaaaaga ctaatttttt tgagatacca cataaagtct
     2521 attttttcta aacccatagc agcaactcct gcagctgaac caatgataag aatactgcct
     2581 ccggtaccgg cacaatatgc taaaaattcc caaaatgtgc catctacagc aaacgcccca
     2641 gccgactgta taggatacat acccatagaa cctgcaacaa gaggaacatt gtcaataatt
     2701 gaagataaaa taccaatgac aatatttatt acatatacat ttttgttggt tatttcatca
     2761 agccaaccac ctaattgagc taaatgtcct gccgattgta acgctgctac tgccgaaaga
     2821 atacccataa aaaagaaaat tgtaggagta tctattcgtt taagaacatt gtatacagta
     2881 agtttctttt tgaattcagg tgcttttttt gcaagcatta tttcagttgt catccaaaga
     2941 atacttagtc caagcaacat gcccatgtat ggaggtaaat gtgtatatgt tttaaaaatt
     3001 ggaacaaaca acaaagcacc tattccaata caaaagatta tttttctatg acgtgccgat
     3061 gtgaattctt tctcgtcatc attttttgca ggtttttcaa cgtttccttg taatatgaaa
     3121 cttaaaatac ctaaaggtac aactaaattc ataatactag gaagaagtac accttttata
     3181 attgccattg cggtaatttg acctccaatc cacagcataa tagttgtaac atcacctatt
     3241 ggcgaccatg caccacctgc atttgcagct attactataa tacttgcaaa aatccatcga
     3301 gtttttttgt cttcaatgag tttgcgtaac agggcaatca ttactattgt agttgttaga
     3361 ttgtctaatg ctgccgacat gaaaaatgtt ataattccta aaatccacag taaacttgat
     3421 ttctttttag cattgattcg gtcagttata attttaaatc cttcgtgttg gtctactact
     3481 tcaactatgg tcattgctcc aagtaagaaa aataaaatct ctgcaatttc aattaaatga
     3541 tgttcaagtt cttcaactat aaaatgtgta atatctaatt taccctcagt ttctatccat
     3601 cccggactaa atcctaaatt taaaatgttt acgctgtcga gcataaagag agtccacaac
     3661 attacgccaa taaataatgc acttgcagct ttgtcaattt taattgtatg ttccaacgct
     3721 atagctgtat agcctagaac aaaaataagt atcataatgt aaaacatagg aatatatttt
     3781 ttgtaatcaa taaaagaact gataaatttt atgtaaaaat atatattatt agaattacca
     3841 cttgtttttt tatagttttt tttttaaatt tttctaaaag ttttgatcac gattttataa
     3901 ggcatgtaag ggggggaata catgcgaaaa tattttttgt aaaatctgtg cacatctgtg
     3961 taattcgcca cggcgaaatt ggtctgtggt gaaaaattag taacaggttt taataactat
     4021 tatgttcaaa gaaaaaaaag tttaccacag atatcataga ttatcacaga ttttgtaatt
     4081 atgaaaatat tgattgtgtt tttgataatt cgctaaagcg gaacatgtat atgaaatctg
     4141 tggagaattt gaaaaataga tgtatttacc ttacttctaa atcagttttg tattaccttt
     4201 gcatttcata gtttgagcaa tcaaattcaa tcaaaacata cctatgaagg tttcaaaata
     4261 tacattttta gtgaaattta ttcggtggcg aatgcaacat gtaaccgata aacaatttgt
     4321 gtattttttg agcataatta tagggttaat cgttggaatt gctgcggtat tattgaagtt
     4381 attggtacac gcaattcgtc atttagttca tatatggtct ccatttcatg aaggtaattt
     4441 tttatatttt ttcttaccta tggttggtat agctgctgct gtgctattta tgaaatatgt
     4501 tataaaacat aatgtaaaac atggtgtgcc aagtgtgttg tatgctattt caatggagca
     4561 aggtaaatta aaaccacatc aaacattttc gtcaattgtt accagtgctc ttactgtagg
     4621 atttggaggt tcagttgggc tagaggggcc aagtattgta accggtgctg ctattggttc
     4681 caatgtagga agaattttgc gactgaaata taaacatata attttactta tagggtgtgg
     4741 ttctgcaggg gtaatggctg caatttttaa tgcaccggta gccgcacttg tatttgctct
     4801 tgaggttatt gtacttaatt tggcaatgag ttcaatagtg ccattattat tggcctcatc
     4861 tgcagctacc ttaacatcat atttttttct tgaacaagat gtgttgtttc atgcggtaga
     4921 attatttaat aagggctatg aattgctcga tataccatcg tttatagtac tgggaatttt
     4981 taccggttta gtatcggtgt attttaaacg aatgtatata gctatagaat ctaagtttga
     5041 acatattgag cgacgaaagc ataaactcta tataggagga ggtttgttag gtttgttgtt
     5101 attctttttc ccttcgttat atggtgaagg gtatgatttt ataaatatgg catttcgggg
     5161 cgattattcc tttttgtttg ccaatagtat ctttataatt tttgaaaaaa gttttctatt
     5221 gacatgtgta ttgttttgtg ccgtaatatt tataaaggtt atagcgtcat cgcttacttt
     5281 tggagcaggg ggagtagggg gtatatttgc tccatcattg tttataggag caaatattgg
     5341 tttatttttt tggtatgtgt ttgaacattt tcaaatagat gccccggtta ctatatttgt
     5401 attacttgga atgtgtggaa ctattgcagg ggttttacat gctccgctta ccggaatatt
     5461 tcttattgcc gatattacca gtggatatag cctgtttgtg ccacttatga tagtctcggc
     5521 agtatcgttt gctacggttc gtatatttga aaataattcg gtatatacgt atttattggc
     5581 taaacgaaag cagttaatta ctcacaacaa agaccaatcg gtattgacac tgttgcagat
     5641 aaaaaaactg attgaaacta attttagtac tgtgcatgta aatgctacat taggtgattt
     5701 aattgaagtt ataaaatatt ctaaacgaaa tatttttccg gtagttgatg atgatggtat
     5761 gttgcatggt atagtaaaat tagatgatat tcgacacata atgttttcac ccgaaaaata
     5821 caacaaaatt gatgttcgca ccgtgatgca catgcctaaa tattattttt ctcctaccga
     5881 tgatatggat gatgtggttg cggtaattca aaaaagtgga cattataact ttccaatcct
     5941 cgacaatgga aaatatgtag ggtttatttc tagagcaact gtcttttcag agtatcgtaa
     6001 aatttctgag tttttttcgg aagagtaaag gtcggtatta attgttaatt tttttatata
     6061 caatttattt ggttaattca gtaaattgta attattttgt aggaattgca actattttgg
     6121 taaaaaaata gtcttacaca tgtattctaa tgtttatttg aaactgtata tatatgaaac
     6181 caacaaaaac tatccctcac agtatgcgta aatttctcgc aacaacgtat ttagtattat
     6241 tgtcggcttt gttagttcct gttttttcac aggtcgattt tgagttttgg tttgcagctc
     6301 cctatgcatc gcagcaacat aaccccaatg cattaggtcc cggaggacga ccaatttatt
     6361 t
//
LOCUS       DAOTOW010000023         6324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_10598_length_6324_cov_3.491306, whole genome shotgun sequence.
ACCESSION   DAOTOW010000023 DAOTOW010000000
VERSION     DAOTOW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10598_length_6324_cov_3.491306"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="P5243_00735"
     CDS             complement(<1..710)
                     /locus_tag="P5243_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRS18000.1"
                     /translation="MYLGTPIYTYQEIVELLHAYIPDISYYTKSFVERRFYYVCSKLY
                     IPSLHAFQEKIQEDITFLDTFMQHFTVPVTEMFRDATVWKNLYTNILPQFKNAKQCAI
                     WLPNCSSGEELYSLSIILKQLQMQNNTIVYASHRSLAGIESIKKGIYTEYNEQLNTLN
                     FAKLQLNTTLDTFYTKSFHSLYMNASLLKNVSFMYSTSILQQIPAQVDIVLFRNSLLY
                     FNVHMHQIILANITKHLR"
     gene            complement(710..1552)
                     /locus_tag="P5243_00740"
     CDS             complement(710..1552)
                     /locus_tag="P5243_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRS18001.1"
                     /translation="MEISHKELLDFISCFKQYSNYDLSEYSENSLMRRLDKVLTDNAI
                     TFDVLLHKISTDAKFCNLVMKQITVNTTELFRDKLMWQELQYKLLPEFEKKSSITIWH
                     AGCSNGLEVYSLAILLHHMNLLDRCRIIATDINPDIIQQAIKGEYSLRLLDEFQIAFD
                     NAIKENPYDSEFKNLHFDTYFEVNRKKEVIAIRQNIKNKPDFSVLDLVKLDDSFPMKF
                     DIVLCRNVLIYFNNSLQKRVFEYFYNHMNSDAYLILGMQESMAWFMNSMFEKKGLFYK
                     KCKE"
     gene            complement(1654..2733)
                     /locus_tag="P5243_00745"
     CDS             complement(1654..2733)
                     /locus_tag="P5243_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRS18002.1"
                     /translation="MADFNSTSKAEQTIRSIKSRQFLIPVFIGFAVVIYMFWGEFDKS
                     GLDEIKLTYYSLLFIICAFCMMLTRDIGYIVRLKILSSGRLTWKQCIRIIFLWEFTST
                     ITPSAVGGTSLAILFIYKEKVTLGESTAMVMTTAFLDELYFLLMFPLLLLTIDIGHLF
                     SVNGSYDGGINWGNQFFYFAVIGYSLKAFFTGVVFYALFINPIGFKRFLAFVFSIRFL
                     KRWKRSAIQTGIDLQFASVELKNKSWKFWLQSFVATFVAWTSRYWVVNFLFLAFFVVP
                     NHMLIFARQLVMWIMMLVSPTPGGSGFAEFIFSEYLGEFIVVGSLIPILVVLWRLVTY
                     YPYLIIGAFLVPVWIKKKFSKSKSN"
     gene            2830..2900
                     /locus_tag="P5243_00750"
     tRNA            2830..2900
                     /locus_tag="P5243_00750"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2862..2864,aa:Cys,seq:gca)
     gene            complement(2945..4015)
                     /gene="mnmA"
                     /locus_tag="P5243_00755"
     CDS             complement(2945..4015)
                     /gene="mnmA"
                     /locus_tag="P5243_00755"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRS18003.1"
                     /translation="MQTKKIVLAMSGGTDSSAAAILLQEQGYEVYGLFIIFYDIQWAP
                     GHVVQQQEVSCNNVKKLCDMLHIPCYIYNASQEFYTTVISYFVVEYLEGRTPFPCAKC
                     NPLLKWKILYDYAVQLNCNYIATGHYVNIELHNEIYYITQGIDSDKDQSFFLWGLTQD
                     ILQKAVFPLGGYTKQQVREYARSKGFVDVATRKDSLGVCFLGNTNYRSFVTRELAKRS
                     VRVEAGKFVDEHGNYISNNKGYVWYTVGQRKQLGLNINTRMFVKSIDAKNNTVTVSPY
                     SSLFRSQFVIHSFYFHTLQDMQGELVVKVRYRNQSNICTLQYNSTESITVILQKPLEA
                     IAPGQTAVFYKNNRVVGGGFID"
     gene            complement(4039..5931)
                     /locus_tag="P5243_00760"
     CDS             complement(4039..5931)
                     /locus_tag="P5243_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS18004.1"
                     /translation="MFKKISILIVCLLPLVVMSQTYKQTKYIVVDQFGYLPESRKIAV
                     IRNPEVGFDASETFKPGKTYAVVEAKTKRQVYKAKPVAWKNGAVDPSSGDMVWHFDFS
                     SVKKEGTYYILDIDKKLISYEFEISNNVYKEVLKQAFRTFYYQRAGFPKIAKYAGEGW
                     ADGASHLGKRQDPNCRQWGLENDASTEKDVRGGWYDAGDFNKYTNWTSDYIIYMLLAF
                     EENPKAWTDDFNIPESGNGIPDIVDEVIFGLEHLLRLQFSSGSVISVVGLDEASPPSA
                     ASKPSYWGAPSTSATFSAVAAWAYGAKVVKQYNEKLAAKLIEAAKLSWEWAEANPNMK
                     FYNNSAQHGTQGLAAGQQEVDDMGLIEKRLQAALRMYDATGDEKYKKIFEDNYKQLKM
                     VAWTLVFPFGEYNQDLLLYYTKLPKADPAIVNHIKAVYKQATDTIHNLFAIKNNDDPY
                     FSYQKDYVWGSNGTKAKQGNIYYNLVQYNIMPEMKDEAKNIAEYYIHYLHGVNPLNKC
                     YLTNMSAYGAENSVNQIYHMWFRHGSKKWDEVGKSTYGPPPGFVTGGPNKEYDWDKCC
                     PQNCDSKENNAMCFELDIKPLKNQPAQKSYMDFNQGWPLNSWAVSENSNGYQVQYLRL
                     LSKFVK"
     gene            complement(6091..6164)
                     /locus_tag="P5243_00765"
     tRNA            complement(6091..6164)
                     /locus_tag="P5243_00765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6128..6130),aa:Arg,seq:tcg)
BASE COUNT         2148 a         1166 c          838 g         2172 t
ORIGIN      
        1 tgccttaaat gttttgtaat atttgcaagt attatttgat gcatgtgaac attaaaatac
       61 aataaactat ttctaaataa aactatatct acttgagcag gaatttgctg caatatagat
      121 gtactataca taaatgatac atttttaaga agtgatgcat tcatatacaa cgaatgaaat
      181 gattttgtat aaaatgtatc taacgttgta ttcagttgta attttgcaaa atttaacgta
      241 tttaattgtt cattgtattc tgtataaatt ccttttttta ttgattctat accggccaaa
      301 cttctatgcg atgcatacac tattgtatta ttttgcattt gtaattgctt aagtataata
      361 ctcaacgaat acaactcttc accactagaa caatttggta accaaattgc acactgtttt
      421 gcgtttttaa attgaggcaa tatattggta tacaaatttt tccaaacagt agcatcacga
      481 aacatttctg taacaggaac ggtaaaatgc tgcataaaag tatcaagaaa tgtaatatct
      541 tcctgaattt tttcttgaaa agcgtgtaga gaaggtatat acaatttaga acatacgtaa
      601 taaaatcttc gttcaacaaa actcttagta taatagctta tatccggaat atacgcatga
      661 agtaattcta caatttcttg atatgtatat attggcgttc ctaaatacac tattctttac
      721 attttttgta aaacaatcct tttttctcaa acatagaatt cataaaccac gccatgcttt
      781 cttgcattcc caatattaaa taagcatcgc tattcatgtg attatagaaa tattcaaaca
      841 cacgtttttg caacgaatta ttaaaataaa ttagtacgtt tctacataaa actatatcaa
      901 atttcattgg aaacgagtca tcaagtttta ctaaatcaag aactgaaaaa tcaggtttat
      961 ttttaatgtt ctgccttatg gcaataactt ctttctttct atttacttca aaatatgtat
     1021 caaaatgtaa atttttaaat tcagaatcgt agggattttc tttgattgca ttgtcaaaag
     1081 ctatttgaaa ctcatcaagt aatcgtaaac tatattcacc tttaatggct tgttgtatta
     1141 tatcgggatt tatatcggta gctattatac gacacctatc caacaaattc atatgatgca
     1201 ataatatagc taacgaatat acttctaatc cattggaaca gcccgcatgc caaattgtaa
     1261 ttgatgattt tttttcaaat tccggtaata atttatactg taactcctgc cacattaatt
     1321 tgtctctaaa caattctgtc gtatttacag ttatttgttt cattaccaaa ttgcaaaatt
     1381 tagcatcagt agatatttta tgcaataaaa catcaaacgt tattgcatta tcggtaagta
     1441 ctttatctaa tcttcgcata agcgaatttt cagaatattc tgacaaatcg taatttgaat
     1501 attgtttaaa acaagagata aagtcaagaa gttctttatg agaaatttcc atgtatttgg
     1561 gtcaaactaa attttatttt gcatatacaa aaatagacaa atactatgag ttaacaaaat
     1621 agatttgcat ttaacataac atatatcatc attttaatta gattttgatt tagaaaattt
     1681 cttttttatc caaaccggaa ctaaaaatgc tccaattatt aaatatggat agtatgttac
     1741 cagccgccat aaaacaacta aaataggaat aagagaacct actacaataa attcaccaag
     1801 atattccgaa aaaataaatt ctgcaaaacc acttcctccc ggtgttggac ttaccaacat
     1861 cataatccac attaccaact gacgagcaaa tataagcatg tgattgggga ctacaaaaaa
     1921 agcaagaaat aaaaaattaa ctacccaata cctggatgtc catgctacaa aggttgcaac
     1981 aaaagactgc aaccaaaact tccacgattt attttttaat tcaaccgaag caaattgaag
     2041 gtcaattcct gtctgaatag cagaacgttt ccaccgttta agaaaacgaa tagaaaacac
     2101 aaaagccaaa aatcgcttaa acccaatagg attgataaac aatgcataaa aaacaacacc
     2161 ggtaaaaaaa gccttaagac tatatccaat aacagcaaaa taaaaaaact gattgcccca
     2221 atttatacca ccatcataag aaccatttac agaaaataaa tggccaatat caatagttaa
     2281 taataacagt ggaaacataa gtaaaaaata cagttcatct aaaaaagcag tagtcataac
     2341 cattgcagta ctttcaccca acgtaacttt ttctttgtaa ataaacaaaa ttgctaaact
     2401 tgtacctccc actgccgaag gagtaatagt tgaggtaaac tcccataaaa atataattct
     2461 tatacattgc ttccacgtta atctaccact tgataaaatt ttaagtctca caatgtatcc
     2521 aatatcacga gtaagcatca tacaaaatgc acaaattata aataacaggg aataatatgt
     2581 aagcttaatt tcatctaaac ccgatttatc aaattctccc caaaacatat agattacaac
     2641 agcaaaacct ataaaaacag gtattaaaaa ttgcctgctt ttaattgagc gaattgtttg
     2701 ttctgcttta cttgttgagt taaaatctgc catgttttgt atatttatta agcaatagct
     2761 gcaatatcgt atattttttt tgctaaaaga aatgaaagtt agaaaaatat attatttttg
     2821 cagcacaatg gttccttggc cgagtggcta ggcaccggtc tgcaaaaccg tatacggcgg
     2881 ttcgaatccg ccaggaacct ctaaaaaact ccgattgtaa aatatcggag ttttttttgt
     2941 attatcaatc aataaaacca ccacctacta ctctattatt tttataaaat actgctgttt
     3001 gtccgggagc tatagcctcc aaaggttttt gcaatataac ggtaattgat tccgtagaat
     3061 tatactgtaa tgtgcatata ttactttgat ttctatatcg aaccttaaca actaattcac
     3121 cctgcatgtc ttgtagtgta tgaaaataaa atgagtgaat tacaaattgc gaccgaaata
     3181 acgatgaata agggcttact gttactgtat tattttttgc atcaatactt tttacaaaca
     3241 tacgtgtatt tatattaagc cccaattgtt ttcgttgtcc aacagtatac cagacgtatc
     3301 ccttattatt tgatatataa ttaccatgtt catcaacaaa ttttcctgct tcaaccctca
     3361 cagaacgttt tgctaattcc cgtgttacaa atgaacgata atttgtattt cctaaaaaac
     3421 aaactccaag actgtctttg cgtgttgcaa catctacaaa ccctttacta cgagcatatt
     3481 cgcgaacttg ttgctttgta tagcctccca aaggaaatac tgctttttgt aaaatatctt
     3541 gcgtaagtcc ccataaaaaa aacgattgat ctttatctga atcaatccct tgtgtaatat
     3601 aatatatttc attgtgtaat tctatattta cataatgacc ggtagctata taattacaat
     3661 tcaattgtac agcataatca tatagtatct tccatttaag caacggattg catttagcac
     3721 aaggaaatgg ggttctacct tctagatatt ctacaacaaa atatgatata acagtagtat
     3781 aaaattcttg tgacgcatta taaatataac aaggtatatg taacatgtcg cataattttt
     3841 ttacattatt acatgatact tcttgctgtt gtaccacgtg gccgggagcc cattggatat
     3901 cataaaatat aataaataat ccatacactt catacccttg ttcctgcaac aaaatagcag
     3961 cggcggaact gtcggttccg ccgctcattg ccaatacaat tttctttgtt tgcatacctt
     4021 gtatggtatt gacttatatt attttacaaa tttcgataac aatcgtaaat actgaacttg
     4081 atacccatta ctattttcac tcacagccca tgaattgaga ggccatcctt gattaaaatc
     4141 catatatgat ttttgagcag gttgattttt gagtggtttt atatccaatt caaaacacat
     4201 tgcattattt tctttactat cacaattttg aggacaacat ttatcccaat catattcttt
     4261 atttggacca cctgttacaa atccgggagg aggcccatac gttgattttc caacttcgtc
     4321 ccatttttta ctgccatgtc taaaccacat atgataaatt tgatttacag aattttcggc
     4381 accataggct gacatatttg ttaaataaca tttattaagt ggatttacgc catgtaaata
     4441 atgaatgtag tattctgcaa tattttttgc ttcatctttc atttcgggca ttatattgta
     4501 ctgcaccaaa ttgtagtaaa tattaccttg ttttgctttt gttccatttg aaccccacac
     4561 ataatctttt tgatatgaaa aataaggatc atcattattt ttaattgcaa ataaattatg
     4621 aatagtatca gttgcttgtt tgtaaacagc ttttatatgg tttactattg caggatctgc
     4681 tttaggtaat tttgtataat acaataataa atcttggttg tactcaccaa aaggaaatac
     4741 taatgtccaa gcaaccattt ttaattgttt ataattgtct tcgaatattt tcttatattt
     4801 ttcatcaccc gtagcatcat acatgcggag tgcggcttgt aaacgctttt ctatcaatcc
     4861 catatcatca acttcttgtt gaccggcagc aagcccttga gtaccatgtt gtgcactatt
     4921 gttgtaaaat ttcatattcg gatttgcttc tgcccattcc catgaaagtt tagcagcttc
     4981 tattaatttc gcagcaagtt tttcattata ttgttttaca actttagcac catatgccca
     5041 tgccgcaacc gccgaaaatg tagccgaagt acttggcgct ccccaataac ttggtttaga
     5101 agccgccgat ggcggcgatg cttcgtctaa tccaacaaca gaaattacag aaccacttga
     5161 aaattgtaac cgcaaaagat gttctaagcc aaatattacc tcatctacta tatccggtat
     5221 tccattacct gattcaggta tattaaaatc atctgtccat gcttttggat tttcttcgaa
     5281 agctaaaagc atataaataa tataatcgct ggtccaattg gtatatttat taaaatcacc
     5341 tgcatcatac catccgccgc gcacatcttt ttcggtactt gcatcatttt cgagccccca
     5401 ttgtctgcaa tttggatctt gacgttttcc taagtgactt gcaccatcag cccagccttc
     5461 acctgcatat ttggcaattt taggaaatcc tgctcgctga taataaaatg ttcgaaaagc
     5521 ttgtttaagc acttctttat atacattatt tgatatttca aactcatacg aaataagttt
     5581 tttatctata tcaagtatat aatacgttcc ttcctttttt accgatgaaa aatcaaaatg
     5641 ccacaccata tcacccgacg atggatctac tgctccgttt ttccatgcta ccggttttgc
     5701 tttatacact tgtcgctttg tttttgcttc aactaccgca tatgttttac ccggtttaaa
     5761 tgtttctgaa gcatcaaatc ctacttcggg atttcttata acagcaattt ttcgcgattc
     5821 aggtaaatat ccaaattgat ctaccactat atactttgtt tgcttatagg tttgagacat
     5881 tacaaccaaa ggcaacaaac aaactataag aatacttatt tttttaaaca taacaataat
     5941 attttatttt ttatacaatt gccacaaaaa tacaaaaact tatgattttt ttacactttt
     6001 atactatcca tttttgatgg aacttacatt catcctgtac acttttccaa ataaaaaaag
     6061 gtaacatttc gttacctttt cttttctctg tgaccccgac aggattcaaa cctgtaaccc
     6121 tcagagccga aatctgatat tctattcagt tgaactacgg agccatttat aatgcaaaaa
     6181 tagatttttt taacataaca gcacaatact tataaaaaaa gagatgaaaa aaatttttca
     6241 tctctttttt tactattatt ttttttcaac tacataccaa atgcataata gaatgtgccg
     6301 ggaacatcag gatacacacc ttct
//
LOCUS       DAOTOW010000024         6211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_10822_length_6211_cov_3.263320, whole genome shotgun sequence.
ACCESSION   DAOTOW010000024 DAOTOW010000000
VERSION     DAOTOW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10822_length_6211_cov_3.263320"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="P5243_00770"
     CDS             complement(<1..1055)
                     /locus_tag="P5243_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRS18005.1"
                     /translation="MYIFIVLYTIVYCLLYIPLFCFAALWNKKIREGLLGRIYQIKNI
                     PSIVTASNNKKVILIHSASVGEWEQSIPIIKQLKQNHTDLYIIATFFSPSGMRHAKKT
                     DIDAALYLPFDVVFTAYFFLNKIRPAVWIISKYDVWPGFVCAAHMRNIPILLCSAELA
                     QDSTRHKGILAYINKLFYSHIHTIFPVSEEYKQHFMRIFPFPERLIVAGDARYDQIIQ
                     KAEQIQSQPAIQLFAHKKQVTFIAGSIWPADEKHLLPAIIKIFKQRNDLQLILVPHEL
                     HENHIAAIQKELLQANIVSERYTEFSKKETDCTTCDVAIIDTIGMLAKIYHISDVAYV
                     GGAFSTGVHNVAEPAIL"
     gene            complement(1062..2096)
                     /gene="waaF"
                     /locus_tag="P5243_00775"
     CDS             complement(1062..2096)
                     /gene="waaF"
                     /locus_tag="P5243_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HRS18006.1"
                     /translation="MKPIQKILVFRLSSIGDIVLTTALLRCLRNTYPNAQIDFVVKRQ
                     FASILQCVPYVSHVYQFDSTAGIKGLLRLRRKLSKQHYDVVLDIHKNWRSTFISYTIG
                     AHKIYRFVKHVLKRYLLIAWKLDAYNVLRPVYLRFIDTARKIGVQNDGKKTELVVPEY
                     AQQIVDNYLEDNGIKPHKNIVVICPGASFTNKQWSEENFLELTKKILKLLPTQVVLLG
                     GYKEKQICESIAAQCKGNVLSVAGVFDLSQSAALLNRAQLTIANDTGMLHMSESLNVP
                     VVGIYGPTVRQFGYFPILEQSRVAQVELECRPCTKMGMNYCPKKTFACMKLIEVDTVY
                     NLAVKIFNSK"
     gene            complement(2096..3934)
                     /gene="glmS"
                     /locus_tag="P5243_00780"
     CDS             complement(2096..3934)
                     /gene="glmS"
                     /locus_tag="P5243_00780"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRS18007.1"
                     /translation="MCGIVGYIGSKEAYPILINGLKRLEYRGYDSAGIALQQPGGITT
                     YKKRGKVSDLEAFIHKKETASHIGIGHTRWATHGEPNDVNAHPHTSMNGKFTVIHNGI
                     IENYSRLQKKLIDRGYSFVSQTDTEILANLIEYIYIKGNISAELSVRLALTKVIGAYG
                     LVIMCSDEPNKLIAARNRSPLVIGVGVNEYFIASDATPIIEHTNRIIYLNDKEVAILT
                     PEDLTLKTILNDSLTPIIQELDLDIEEIEKGGFEHFMLKEIYEQPKSINDTFRGRASQ
                     NPPEIYLGGLYSVFDKLDTAQRIIIIACGTSWHAGLVGEYLIEDFARIPVEVEYASEF
                     RYRNPIIYNTDIVIAISQSGETADTLAAVRLAKEAGATVLGICNVVGSSITRETDAGV
                     YTHAGPEIGVASTKAFTAQVTVLTMMALLLGKRRNTIAEQEYKELIAELYSIPKKIEE
                     ILTHNEDYKSIAELYKNATNFIYLGRGCLFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDENMPVVIIATKDKSYEKVVSNIQEVRARKGIVIAIVSKGDKTITGLANHVL
                     EVPETHMQLAPLTTVIPLQLLSYYIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(3936..5201)
                     /locus_tag="P5243_00785"
     CDS             complement(3936..5201)
                     /locus_tag="P5243_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18008.1"
                     /translation="MNNSRICIIACFVSIAMLFSCNKEKIPFGTEVLQDDDFIGAYVD
                     STSVTVSYIPVKDDSIRSSKITYMIGSTSFADGTQTRADFMSRFYFTSLTAPLKECTL
                     DSICFIMYERSQYFGDTNKTQTIQIYELTDSITKNHCKAYYEDAVIPQNLVETGLLIG
                     QKSYKPVFDTSSNFSFKFPQQYAANVFARIQAIYDQTKDVQTIDSLFIKTFKGLYVTS
                     AYQNAAILNVYPQISIYVSKGTTKYRVDLAPTATPYTATSTKDPSLVYLQALSMFHHI
                     YSTEVTSTLNSKGDVAYVQGFSGIKTRVALQGIGAWQDSSVVFNSIKLYVPYVLDTKP
                     NFAPAKMPQLLVYNPLGTKVLTLECYTYDSTYYIYNLNALMPQANNNKYNPNNYMFEL
                     VMPENNLYGNMLKIDALVKQSKLKIIYTK"
     gene            complement(5213..6022)
                     /locus_tag="P5243_00790"
     CDS             complement(5213..6022)
                     /locus_tag="P5243_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRS18009.1"
                     /translation="MKKARVLFVSQEMAKYMPDTEMSLICRNLPQYIQENGKEIRCFM
                     PKYGLINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQAARLQVYFIDNEEFFQR
                     KALFADDSGKEFDDNEDRMIFFARGVLETVKKLRWSPDIVHCQGWFTSLLPLYLKKTF
                     YEDPLFAKSKVVYSLYDNTFTRTFSKDFIKKVHFDGISEQDLQSITKGADWLAINKLA
                     IDQADGIIYGSSTIHPELIAYSEASKKPILPYQSPEDYMQAYSDFYDSLLD"
BASE COUNT         1928 a         1040 c         1037 g         2206 t
ORIGIN      
        1 ttaagtatag ccggttcggc tacattatgc actccggtag aaaatgcacc tcctacatag
       61 gccacatcac ttatatggta tattttggca agcattccaa tagtatctat tattgcaaca
      121 tcacaagtag tacaatcagt ttcttttttc gaaaattccg tatatcgttc acttacaata
      181 tttgcttgca ataattcttt ttgaatagca gctatatgat tctcgtgtaa ttcatgagga
      241 actaatatta attgtaagtc atttcgttgt ttaaatattt ttattatagc aggcagtagg
      301 tgtttttcgt cggcaggcca tatactacct gctatgaatg ttacttgttt tttgtgtgca
      361 aatagttgta tagcaggttg tgattgtatt tgttcggctt tttgaataat ttgatcgtag
      421 cgagcgtcac cggctactat taaacgttca gggaaaggaa aaattcgcat gaagtgttgt
      481 ttatattctt cagaaacagg aaaaatagtg tgaatatgag aataaaataa tttattgata
      541 tatgctaaaa tacctttgtg tcgagttgag tcctgtgcta attctgctga acataataga
      601 atagggatat tacgcatatg tgccgcacac acaaagcctg gccatacatc gtatttagaa
      661 attatccata ctgccggacg aattttattg aggaaaaaat atgcagtaaa tacaacatca
      721 aatggtaaat agagagcggc atcaatatct gttttttttg catgtcgcat acccgaaggc
      781 gaaaaaaatg ttgcaattat atataaatcg gtatggttct gttttagttg tttaattatt
      841 ggaattgact gttcccattc acctaccgac gcactatgta taagaattac ttttttgttg
      901 tttgaagctg taacaatact tggtatattc tttatttgat aaattctgcc aagtaatcct
      961 tcacgtatct ttttattcca aagagctgca aaacaaaata atggtatata aagtaggcaa
     1021 tatacaattg tatacaatac tataaaaata tacatatgta attatttaga attaaagatt
     1081 tttacagcta agttgtaaac agtatcaact tctattaact tcatacaagc aaaggttttt
     1141 ttcggacaat aattcatacc catttttgta catggtctgc attctaattc tacctgagct
     1201 actctacttt gctcaagtat agggaagtat ccaaattgac gaacagttgg cccgtatata
     1261 cctactacgg gtacattgag cgattcagac atgtgtaaca tgccggtatc gttagctatg
     1321 gtaagttggg ctctattgag caatgcagca gattgactta aatcaaatac tccggctaca
     1381 cttaatacat tacctttgca ttgtgctgct atagattcac atatttgttt ttctttgtat
     1441 cctcccaata gaacaacttg ggttggtaat agtttgagta ttttttttgt aagttccaag
     1501 aaattttctt cactccattg tttatttgtg aaacttgcac cgggacaaat aactacaata
     1561 tttttatggg gttttatgcc attatcttct aaataattgt caacaatttg ttgagcgtat
     1621 tcgggaacta ccaattcggt ttttttccca tcgttttgta cgccaatttt acgtgcagta
     1681 tctataaagc gaagatatac agggcgaaga acattatatg catctaattt ccatgcaatt
     1741 agtaaatacc gtttcagtac atgttttaca aatctataaa tcttgtgggc acctatggta
     1801 tatgatataa acgtacttcg ccagttttta tgaatatcaa gtactacatc gtagtgttgt
     1861 ttactcagtt ttcgtcgtag tctcaataat cctttgatac cggcagtaga atcaaattga
     1921 tatacatgtg aaacatacgg cacacattgc agaatcgaag caaattgacg ttttactaca
     1981 aaatctattt gagcattggg gtatgtattt cgtaaacaac gaagcagagc agtagtgagt
     2041 actatatctc ctatagaact gagtctgaaa acgaggattt tttgtattgg tttcactatt
     2101 ctacggtaac agattttgct aaatttcttg gttggtcaac attgcaacct cgcattacgg
     2161 caatataata tgataaaagt tggagtggta ttaccgttgt aagcggagcc aattgcatat
     2221 gtgtttcagg aacttccaat acatgatttg caagccctgt aatagtttta tcgcctttgc
     2281 ttactattgc tattactata cctttacgtg cacgaacttc ttgaatatta cttactactt
     2341 tttcatatga tttatcttta gtagcaataa ttactaccgg catattttca tcaattaagg
     2401 ctattggtcc atgtttcatt tctgctgcag ggtatccttc ggcatgaata tacgaaatct
     2461 ctttcagttt aagggctcct tctaatgcta ccgggaataa gcaacctctg cctaaataaa
     2521 tgaaattagt agcgttttta tacaattcgg caatagattt ataatcttca ttatgtgtta
     2581 atatttcttc aatcttttta ggtattgaat ataattcggc tattagttct ttatattctt
     2641 gttcagcaat agtgttacga cgttttccaa gcaacaatgc catcatggtt aatactgtaa
     2701 cctgagctgt aaatgcttta gtagaagcta cccctatttc gggccctgca tgagtatata
     2761 ctccggcatc ggtttcgcgt gtaatgctgg aaccaaccac attgcatatt cctaaaactg
     2821 tagcacctgc ttcttttgct aaacgtacgg cagccaatgt atcggcggtt tcaccgcttt
     2881 gacttattgc tattactata tctgtattgt aaattatagg atttcggtat cgaaattccg
     2941 aagcatattc cacttcaacc ggtattcggg caaagtcttc aattaaatat tcaccaacca
     3001 gtccggcatg ccacgaagta ccacaagcaa ttattataat tcgctgagct gtatctaatt
     3061 tgtcaaacac actgtataat ccgcctaaat atatttcagg tggattttgt gatgctcttc
     3121 ctctaaatgt atcattgata gatttgggtt gttcatatat ttctttaagc ataaaatgct
     3181 caaatccgcc tttttctatt tcttctatgt caagatccaa ttcttgaatt ataggggtaa
     3241 gagaatcatt aaggatagtt tttaatgtta aatcttcggg tgtaagtata gctacttctt
     3301 tatcatttaa atatatgatt ctgttggtat gttctatgat aggtgtagca tcagaagcaa
     3361 taaaatattc atttacacct acaccaataa ctaatgggct tcgattacgg gcagcaatta
     3421 atttatttgg ttcatcagaa cacattataa caagtccgta tgctcctata acttttgtta
     3481 atgccaatcg taccgaaagt tcggcagaaa tatttccttt tatataaata tattctatta
     3541 aattagctaa tatttcggta tctgtttggc taacaaatga atatcctcta tcaattaatt
     3601 ttttttgtaa tcgactatag ttttcaatta taccattatg aattacggta aatttaccat
     3661 tcattgatgt atgagggtga gcatttacat catttggttc accatgtgtt gcccaacggg
     3721 tatgtccaat accgatgtgc gatgcagttt cttttttatg tataaaagcc tctaagtcag
     3781 atactttacc acgttttttg tatgtagtta ttcctccggg ttgttgcagt gctatacctg
     3841 ccgaatcata tcctctatac tctaagcgtt ttaaaccatt tattaaaata ggatacgctt
     3901 ctttactccc tatgtaccca actataccac acatattatt tagtataaat aatttttaat
     3961 ttagattgtt taactaacgc atcaattttc agcatgtttc catacaaatt attttcgggc
     4021 attaccaatt caaacatata attatttggg ttatatttat tattgtttgc ttgaggcatt
     4081 aatgcattca aattgtatat ataataagta ctgtcgtaag tataacattc aagtgttaat
     4141 acttttgttc ctaaagggtt atacactaat aattggggca tttttgcagg ggcaaaatta
     4201 ggtttagtgt caagtacata tggcacatat agttttatag agttaaacac aacgcttgaa
     4261 tcttgccatg caccaatgcc ttgcaatgca acacgtgttt tgatgccgct aaatccttga
     4321 acatatgcaa catctccttt gctgttgagc gttgatgtaa cttcggttga atatatgtga
     4381 tgaaacattg acagagcttg caaataaact aaactcgggt cttttgtaga tgtagctgtg
     4441 tatggagttg ccgtaggggc caaatcaaca cggtatttgg tagttccttt gcttacatat
     4501 atagaaattt gcggatatac attaagaatt gctgcgtttt gatatgctga tgttacatat
     4561 aaacctttga atgttttgat aaatagtgaa tcaattgttt gtacatcttt ggtttggtca
     4621 tatattgctt gaattcgagc aaatacattt gctgcatatt gttgaggaaa tttaaaacta
     4681 aaatttgaag atgtatcaaa aaccggttta taggattttt gaccaattag taaacctgtt
     4741 tcaacaagat tttgtggaat aacagcgtct tcataatatg ccttgcaatg attttttgtt
     4801 atactatcgg taagctcgta tatttgaata gtttgtgttt tattagtatc gccaaaatat
     4861 tgtgagcgtt cgtacataat aaaacaaatt gaatctaatg tacattcttt gagaggagcg
     4921 gtaagagatg taaaataaaa tcgcgacata aaatcggcac gggtctgggt accatcggca
     4981 aaggaagtac ttcctatcat atacgttatt tttgaagaac gtatactgtc gtcttttaca
     5041 ggaatatatg ataccgtaac cgacgtagag tcaacatatg cacctataaa atcatcatct
     5101 tgcaatactt ctgttccaaa tggaattttt tctttgttac acgaaaataa cattgctata
     5161 cttacaaaac atgcaataat gcatatacgt gaattattca tatacaataa aattagtcta
     5221 ataaactatc gtaaaaatca gaatatgcct gcatgtagtc ttcgggtgat tgatacggaa
     5281 gtattggttt ttttgatgct tcactatatg caatgagttc aggatgaata gtactgctgc
     5341 cgtaaataat tccatcagct tgatctatag caagtttatt tattgctagc caatcagcac
     5401 ctttggttat tgattgtaaa tcttgttctg aaataccgtc aaaatgtact tttttgataa
     5461 aatctttaga aaatgtacgg gtaaaggtat tatcatataa agaatatact actttggatt
     5521 ttgcaaacaa cggatcttca taaaaggttt ttttcaaata caaaggtaat agagaggtaa
     5581 accatccttg acaatgaaca atatcgggcg accaacgaag tttttttacg gtttccaata
     5641 ctccacgagc aaaaaatatc attctgtctt cattatcatc aaattcttta cccgagtcat
     5701 cggcaaacag tgcttttctt tgaaaaaact cttcattatc aataaaatat acttgaagtc
     5761 gagcagcttg aatagaagca acttttataa taagcgggtg gtctgtgtca tcaattatta
     5821 aattcattcc cgataagcga ataacttcat gtaattgatt tctacgttca ttgattaagc
     5881 catatttagg cataaagcaa cggatttctt ttccgttttc ttgaatatat tgaggcagat
     5941 ttctgcaaat aagtgacatc tcagtatcgg gcatatactt tgccatttct tgagatacaa
     6001 ataatactct tgctttcttc atgtaatttt tacttgaaaa tcaatttaaa tacgttacaa
     6061 aagtacaaaa attattgcac atataaaaaa tgacaagtaa aacttattta aattacaaaa
     6121 aaagttattt ttgtcgccta tttatgtatc tgaagtaaaa tatatgataa tttgtaaaaa
     6181 cagaacggac ctgcaatctg ttattaccaa a
//
LOCUS       DAOTOW010000025         6111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11038_length_6111_cov_3.652246, whole genome shotgun sequence.
ACCESSION   DAOTOW010000025 DAOTOW010000000
VERSION     DAOTOW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11038_length_6111_cov_3.652246"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2283
                     /locus_tag="P5243_00795"
     CDS             <1..2283
                     /locus_tag="P5243_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS18010.1"
                     /translation="EKAILTVIPKSLVPLQKTQTIQLSDVLLQGKSIFSADTKTKVEY
                     IDNQYHIYLSGKEDNIAIYFSDFEWNIQNNNEYEYILENYMDDWALLHDANFVNFTKL
                     SGGDYVLRIKKKYQDSSSHDELVVHIHIQKAFWEYWFFYVSLVLLLIGLIYIIYIVRV
                     QSIISMRNSLQQQVEQRMSEIQNKIERIQEQEERIRQQDADFLIQQEIQSKLEQDIET
                     QKQTYELAIQQKDIEIEESKKQTQAVSELKELVEKNFHLLENNTREMVFRIILPSEMF
                     DYVSPAVESFTGYTPQEFYSDSMMFRKLIISEGKDDFKKFRKYMIEGKVPPVMEYKII
                     TKSGKEKWVAQYSTIIRDAKQQPIALEAVLVDISEKKELEQRNNALKKRAKKLEKIEG
                     DFSETQNINTVKQALHVFADLLKREDVSLDEKQAFLESNQESGSFALHMIENFIDISK
                     IEAGKIHFNYSQCYVNTVLQELLDSFTESKQYIGKKHIQLTMNVPSLEENFSFYTDTF
                     RFRQVMMNLLGNAIKFTHSGSVEFGYEIIENVKAENNIELVFFVKDTGEGIPAEKLDL
                     LFNTDMQSEDSEFMGGLGLTVSKKIVELLGGRMWAESELEKGTIIRFTLPVEKMKGFK
                     KAEQQVTEKLVETIDWSQKTLLLVEDEENNYDLVREVLAKTNIQILWAQNGEEGVEMF
                     KQNQRDIDVILMDIQMPIMNGYTATQEIKKIDKDIPIIAQTAYANYDSKLNCIKVGCE
                     NYIEKPYKRKDLIDMLSKYL"
     gene            2299..3381
                     /locus_tag="P5243_00800"
     CDS             2299..3381
                     /locus_tag="P5243_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRS18011.1"
                     /translation="MKLFLNYKFIAVILLPFFCFFIVWIFIRKPTIDLLNSHEYVIHA
                     FSDSLVNGTSTSSVTHKNNAIDVFFELKKGYEFPFAGVQIERADAELFDISDYIVHII
                     IETKNDMRLSVRYNQFIPSYSDSINTLTYALYVKTFTIHKGVNTFECKTKDINEIPEW
                     WYAMNPRIVNNVPSHNLTQTKYMWLYTESTIPLNTPFQFTIRKFTLSYSVQPLLVIFL
                     YVSVLYYFILLIVWRFKKQSISRLFLPIEHITIPEKSNNDFNRIVTYIAKEYSNPDLK
                     LSHVAKSIGIHEDQISEILKHHVHVNFKQYVNKIRIEEALHLLQESKLSISEIAFAVG
                     YNNVQHFNRVFKERMNCSPGTYRESL"
     gene            3485..>6111
                     /locus_tag="P5243_00805"
     CDS             3485..>6111
                     /locus_tag="P5243_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS18012.1"
                     /translation="MKVIHVFFVTGIFCCISLFGISQTLIRPSHNYVQVTGRVDTSNP
                     LKYSFAFPGVSIKTAFSGTGISILLKEYGLGTATTTNYFAVIIDGGEPTVLQLSKNQT
                     EYVLASNLPKGNHTVEVFKRTESNVGKVDFMGFTLPKGTTIIEPAPLPKRKIEFIGNS
                     ITCGYGNEISTTTPDKYHFTSANENNYMAWGAVTARKVQAQYSCVAYSGRGLMQNNTG
                     SKTGTLPLIYDNIIADSPSPVWNHYDYIPDVVVINLGTNDFAAEVSSATYKVDSTQFV
                     QTYIAFIQKLRSYYPNASFICCVGTMMSDYYPVGGKHWSRIQNYVSSVAQYFNSQGDS
                     KVYYLMLDPQLSPYGEDWHPTKKTHEQMATKLTNFINTTITWNDCSASVHVGPDINLF
                     YEQTPITISSNSELLDNVTYSWYKNGELLSGENGVSITITDTVGAIGEYVVVRDSAGC
                     KYHDNIILSNNQISVGEVCKWRDNKCAAIALTFDDWSPGHPAIVVPELKKRGMVSTFN
                     VFTNSVSNWTQIQEEANYGNEIANHTKSHPDLTKISLEALDSEIRFPDSVINSRITNQ
                     KVRTLAYPFGTFNSDIIAYLKSMNYIAARGVWGISNYSYNFVSNEQDYYNTKVYSLGT
                     NTTIQQFGREVYNVVNGGGMLTFLYHSVNSPTVIDNNYAAVPEKEFQYQLDTIQAFAR
                     KVWVGTYSDIITYHRQAKCASITQIQAPHSGTWIIELNDTLSNNQLYKLPLSITLPMN
                     GVSYNSIKQNGQFIPIDSIFNDTIMFKAIPDAGAIELSVLPISVDVQLPITVFTNTLQ
                     QQLMCTVVATGNNGIASVEFDFSELHGSKISATQSSLPNTYLGNYTIPAGIELGYKRI
                     YITIVDNAGNIYLHHVGVT"
BASE COUNT         2182 a          867 c         1047 g         2015 t
ORIGIN      
        1 gaaaaagcaa tacttactgt aataccaaaa tcattagttc ctcttcaaaa gacacaaaca
       61 attcaattat ctgatgtttt attacaaggt aaatcaattt tttctgccga cacaaaaaca
      121 aaggtagaat atattgacaa tcaatatcat atatacttat ctggtaagga agataatatt
      181 gcaatatatt tttcagactt cgaatggaat attcagaata ataatgagta tgagtatata
      241 cttgaaaatt atatggatga ctgggcactg ttgcatgatg ccaattttgt gaattttaca
      301 aaactttccg gaggtgatta tgtgctgcga ataaagaaga aatatcaaga ttcttcttct
      361 catgatgaat tagtagttca tattcatatt caaaaagcat tttgggaata ttggtttttt
      421 tatgtttcgt tggttttatt acttatagga ttaatttata ttatatatat agttagagtt
      481 caatctatta tttctatgag aaattcattg caacaacaag tagagcagcg aatgagtgaa
      541 atacaaaata aaatagaacg aatacaagaa caagaagaga gaattagaca acaagatgca
      601 gactttttaa tacaacaaga aattcaatcg aagttagaac aagatattga aacccaaaag
      661 cagacatatg aacttgcaat tcaacaaaaa gatatagaaa tagaggagtc taaaaaacaa
      721 acgcaagcag tatctgaatt aaaagaactt gttgaaaaaa actttcattt actcgaaaac
      781 aatactcgag aaatggtatt tagaattatt cttccttcag aaatgtttga ttatgtaagt
      841 cctgccgtag aatcgtttac aggctatacg cctcaagaat tctactctga tagtatgatg
      901 tttagaaaac ttataatttc tgaaggtaaa gatgatttta aaaaattcag gaaatatatg
      961 attgagggca aagttcctcc tgtaatggaa tataaaatta ttaccaaaag cggaaaagaa
     1021 aaatgggttg cccaatatag taccataata cgcgatgcca aacagcaacc aatagcttta
     1081 gaagctgttt tagttgatat ttctgagaaa aaagaattgg aacaaagaaa taatgcctta
     1141 aaaaaacgtg caaaaaaatt agaaaaaata gaaggagatt tttctgaaac acaaaatatt
     1201 aatactgtaa aacaagcatt gcatgtattt gccgatttac tgaaacgtga agatgtttct
     1261 cttgatgaaa aacaagcatt tttagagtca aatcaagaaa gtggttcgtt tgcattgcac
     1321 atgattgaaa attttattga tatttcaaaa atagaggccg gtaaaataca tttcaattat
     1381 tcacagtgtt atgtaaatac tgtgttgcaa gaattactcg attcgtttac cgaaagtaaa
     1441 cagtatatcg gtaaaaagca tattcaactt accatgaatg tgccgtcgtt agaagagaat
     1501 ttttcatttt atactgatac attcaggttc agacaggtaa tgatgaattt gttgggtaat
     1561 gctataaagt ttactcattc aggttctgtc gaatttggtt atgaaataat tgaaaatgta
     1621 aaagcagaaa ataatataga attagtattt tttgtaaaag atacaggcga aggtattccg
     1681 gctgaaaaat tagatttgtt attcaataca gatatgcaat cagaagactc tgagtttatg
     1741 ggaggtctcg ggttaacagt ttcaaaaaaa atcgtggagt tgttaggtgg tagaatgtgg
     1801 gctgaatctg aacttgaaaa gggaactatt atacgattta cgttgcctgt tgaaaaaatg
     1861 aaaggattca aaaaagcaga acaacaagta accgaaaagc ttgttgaaac tattgattgg
     1921 tcgcaaaaaa ccttgttgct tgttgaagac gaagaaaata actatgacct tgtacgtgag
     1981 gtgttagcaa aaactaatat tcagattttg tgggctcaaa atggtgaaga aggtgtagaa
     2041 atgtttaaac aaaatcaaag agatattgat gttattctta tggatataca aatgcctata
     2101 atgaatgggt atactgcaac acaagaaatt aaaaaaattg ataaagatat tcctataatc
     2161 gctcaaacag catatgctaa ttatgattca aaattgaatt gtattaaagt aggatgcgaa
     2221 aattatattg agaaacctta taaacggaag gatttgatag atatgctcag taaatattta
     2281 taacagtttt ggtgtatcat gaaattgttt ttgaattata agtttattgc ggttattctc
     2341 ttaccttttt tttgtttttt tattgtttgg atttttattc gtaaaccaac tattgacttg
     2401 ctgaattctc atgaatatgt aattcatgct tttagtgact ctttagtaaa tggtacttct
     2461 acgtcatcgg taactcataa aaataatgca attgatgtgt tttttgagct caaaaaagga
     2521 tatgagttcc cttttgcagg tgtgcaaata gaacgtgccg atgcagaatt atttgatatt
     2581 tctgattata tcgtgcatat tataattgaa actaagaatg atatgcgtct atcggttcgc
     2641 tataatcaat ttataccatc atacagtgat tctatcaata cattaacata tgcattgtat
     2701 gtaaaaacat ttactattca caaaggggtg aatacgtttg aatgtaaaac aaaagatata
     2761 aatgaaatac ccgaatggtg gtatgcaatg aatcctcgta ttgtaaataa tgttccatct
     2821 cataatctta cccaaactaa atatatgtgg ttgtataccg aaagtactat acctcttaat
     2881 actccatttc aatttactat acgtaagttt actttatcgt attctgttca accgttattg
     2941 gtaatttttt tatatgtatc tgttttgtat tattttatac ttcttatagt atggcgattc
     3001 aaaaaacaat ctattagtcg attgttttta cctattgaac atattacaat ccctgaaaaa
     3061 tcaaataatg attttaatag aatagtaacc tatatagcaa aggagtattc aaatcctgat
     3121 ttaaaacttt cacatgtagc taaatctata ggtatacatg aagatcaaat ttcggaaatt
     3181 ttaaagcatc atgtacatgt aaatttcaag caatatgtga ataaaattcg tattgaagaa
     3241 gctttgcatt tgttacaaga atcgaaactt agtatatcag aaattgcatt tgcagtggga
     3301 tataataatg ttcaacattt taatagagtg tttaaagaac gtatgaattg ttcaccagga
     3361 acatatagag aatccctata atatattgat ttacataaaa tgttaagtct ttagggaatt
     3421 tttgttaagc tattgtataa actttcaatt acctttgtta taaactttaa aaaattggca
     3481 tactatgaaa gttatacacg tattttttgt tacaggaatt ttttgttgca tttcgttatt
     3541 cggaatttca caaacactta ttcgtccgtc gcataattat gtgcaagtta caggtagggt
     3601 tgatacatca aatccgctaa agtattcctt tgcatttccc ggtgttagta ttaaaactgc
     3661 ttttagtggt accggtattt caattttact taaagaatat ggattgggaa cagctactac
     3721 taccaattat tttgcagtaa taatagatgg tggcgagcca acggtattac aactttcaaa
     3781 aaatcagacc gaatatgtac ttgcttcaaa cttacctaaa ggtaatcata ctgttgaggt
     3841 gtttaaacgt accgaatcaa atgttggaaa ggttgatttt atgggtttta cattacctaa
     3901 aggaacaact attatagaac cggctccact acctaaaaga aaaattgagt ttattggtaa
     3961 ttctattacc tgtggatatg gtaatgaaat atcaactact actcctgata aatatcattt
     4021 tacctctgcc aacgaaaata attatatggc atggggtgct gttactgcac gtaaagttca
     4081 agcccagtat tcgtgtgtag catattctgg tagggggtta atgcaaaaca acaccgggtc
     4141 taaaacaggt acattgcctc tcatttacga taatataata gctgatagtc cttctccggt
     4201 gtggaatcat tatgattata ttcccgatgt agttgttata aatctaggca ccaatgattt
     4261 tgctgccgaa gtttcgtcgg caacatacaa agtagattca acacaatttg tacaaacata
     4321 tatagcattt attcaaaaac ttcggtcata ttatcctaat gcttcattta tttgttgtgt
     4381 gggaactatg atgagtgact attatccggt aggtggaaaa cattggtctc gtatacagaa
     4441 ttatgtgtcg tcggtagcac aatattttaa ttctcaaggt gactctaaag tgtattacct
     4501 tatgctcgat cctcaattgt caccttatgg agaagattgg catccaacta aaaaaacaca
     4561 tgaacaaatg gctacaaaac ttacgaattt tattaatact actattacat ggaatgattg
     4621 tagtgcatca gtacatgtag gtcccgatat aaatcttttc tatgaacaaa ctcctattac
     4681 aatttcatca aattcagaat tgttagataa tgttacatat tcttggtaca aaaatggtga
     4741 attactatcc ggtgaaaatg gtgtaagtat tactataaca gatactgttg gcgctatagg
     4801 tgagtatgtt gtggtacgtg atagtgcagg atgtaaatat catgacaata taatactatc
     4861 aaataatcaa atatcagtag gtgaagtgtg taaatggcgt gataataaat gtgctgctat
     4921 tgctctaaca tttgacgatt ggtctcccgg acatcctgct atagttgtcc ctgaactcaa
     4981 aaaacgtgga atggtaagca catttaatgt atttactaat tctgtttcaa attggacaca
     5041 aattcaagaa gaggctaatt atggtaacga aattgcaaat cataccaaat cacaccccga
     5101 tcttacaaaa atttctttag aagcattaga ttctgaaata cgatttcctg atagtgtaat
     5161 aaattctcgt attacaaatc aaaaggttcg cactttggca tatccatttg gtacatttaa
     5221 ttctgatatt atagcatatt taaaatctat gaattatata gctgctcgcg gtgtatgggg
     5281 tatttcaaac tattcatata attttgtgtc aaacgaacaa gattattaca ataccaaagt
     5341 atattcttta ggaacaaata ccacaattca acagtttgga cgcgaggtat ataatgtggt
     5401 aaatggggga ggtatgctta cctttttata tcatagtgtg aatagtccaa ctgtaattga
     5461 taataattat gctgctgttc ccgaaaaaga atttcagtat caattagata caattcaggc
     5521 atttgctcgt aaagtatggg taggaacata ttctgatatt ataacatatc atcgtcaagc
     5581 taaatgtgca agtattacac aaatacaagc accacactcc ggtacgtgga ttatagaatt
     5641 gaacgatact ttgtcgaata atcaattgta taaattgcca ttaagcataa ctttgcctat
     5701 gaatggtgta tcatataata gtattaaaca aaatggacag tttattccta tcgattcgat
     5761 atttaacgat actattatgt ttaaagcaat tcccgatgca ggtgctatag aattaagcgt
     5821 attaccaatt tcggtagatg tgcaactccc tattacagtg tttacaaaca ccttacaaca
     5881 acaactaatg tgtaccgtag ttgctaccgg aaataatgga atagcatcgg ttgaatttga
     5941 tttttcggaa ttgcatggct caaaaataag cgcaacgcaa agttcgttac caaacacgta
     6001 tttgggaaat tatactatac cggcaggaat tgaattgggg tataaacgaa tttatattac
     6061 tattgtagat aatgcaggta atatatattt acatcatgtt ggagtaacaa t
//
LOCUS       DAOTOW010000026         6099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11063_length_6099_cov_3.223031, whole genome shotgun sequence.
ACCESSION   DAOTOW010000026 DAOTOW010000000
VERSION     DAOTOW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11063_length_6099_cov_3.223031"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="P5243_00810"
     CDS             <1..697
                     /locus_tag="P5243_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS18013.1"
                     /translation="KPITNTKQKALVEFKGRTLLEYAILKLKYYGVSDIIINVHHFSK
                     QIIDYVNIHDFGVRITISDESDELLDTGGGLLKAKDFFKDADSFIVYNVDVISTINLL
                     DFYKFHISNNALVSLAVCKRNTSRYLLFDTSKQLCAWENTQSHEMQIIHEREEYFQLA
                     FSGIQIISTKIFDLISEEGAFPIMQLYLRLAQQHKILAYNHSQDFWLDVGKFDKLQDV
                     EKQIANQPLEYLS"
     gene            694..2436
                     /gene="recJ"
                     /locus_tag="P5243_00815"
     CDS             694..2436
                     /gene="recJ"
                     /locus_tag="P5243_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRS18014.1"
                     /translation="MKQYTWVEPEISKQVDEIELWSKQFGIHAKLVPLLWNRGILTFE
                     NAKIFFTPSTSNLHNPFLMADMQKAVDRLALALDTNQKILVYGDYDVDGTTAVAMVYS
                     FLKSCNAQVKYYVPDRYTEGYGVSIQGMDYAIQHSCNLIITLDCGIKAHRAIEYVTQA
                     GIDVIVCDHHEPAETLPPAYAVLDPKRDDCNYPFKELSGCGVDYKFLYALCEQRSISI
                     KENLNCYMDLLAISIASDIVPIVDENRVLIYFGLLKIMRKPHLGIKAITETSGIFGPI
                     TVSDVVFKIGPRINAAGRMYNAKTVIELLISDNYEKAVDICKQIQEYNISRRAIDKSI
                     TDEAIAMLENDEQTAFKKTNVLYKPSWHKGVIGIVASRVLEQFYKPTVIFCGEGDIIS
                     ASARSVAEFDLYKALEQCSDLLVNYGGHTFAAGMSMEKQYLPQFIERFETIVSQSITE
                     DALQPKILIDAELNIDDITLQLYEDMMRFAPFGPQNMTPVFMIRGLCDTGKSKIMGND
                     QRHVKLELMSSGKSTKSIEAIGFGLAHTWKQISKTHSNIDICFTIREHEFRNIKSLQL
                     ELRDIRPHIDYSQV"
     gene            complement(2421..2651)
                     /locus_tag="P5243_00820"
     CDS             complement(2421..2651)
                     /locus_tag="P5243_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18015.1"
                     /translation="MKKNILFIVIPFLITISACVDPANECAQCYSVKENTTNGAIEAK
                     SSEGTKCGTELDSIESLQPEYLDEYKYYYTCE"
     gene            2733..3788
                     /gene="rfbB"
                     /locus_tag="P5243_00825"
     CDS             2733..3788
                     /gene="rfbB"
                     /locus_tag="P5243_00825"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRS18016.1"
                     /translation="MKKTILITGGAGFIGSHVVRRFVTQYPEYTIVNLDLLTYAGNLE
                     NLKDIENKPNYIFERGDIADVEKVHQLFETYKFDGVIHLAAESHVDRSITDPFSFVRT
                     NVYGTVVLLNEAKNAWKDNYDGKRFYHVSTDEVYGSLHDGGFFVETTPYDPQSPYSAA
                     KASSDHFVRAYANTYKLPIVISNCSNNYGPNHFPEKLIPLCINNIMNKKPLPIYGKGD
                     NVRDWLYVIDHAIAIDLIYHKAEAGETYNIGGNNEWTNLNLVKKLCEIMDTKLGRTPG
                     ESAQLITFVKDRAGHDLRYAIDSSKLMTKLGWKPSVTFEQGLDITVDWYLQNQEWLRN
                     VVSGNYEMYYETMYKQR"
     gene            3791..4660
                     /locus_tag="P5243_00830"
     CDS             3791..4660
                     /locus_tag="P5243_00830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS18017.1"
                     /translation="MRNWIFYGLIIVFFWACSSENSKKYESTENGLEYKYCVKSESNK
                     KPQSGDKLLLKVRFYNEKDTLLWDSREISSRFIMDFVQPQSPGNTVNDAYAMMHEGDS
                     MSFLINARQFYSAADPKSPMIQKFRDDEKLRFQIKLEKIYSLSEFEEEAKKVIKPLCE
                     EEKMLLEDYIEKQYPEAKPTQSGLYIIHTKQGAGKQVNNQDEVAIHYRATYINGELIY
                     TTYAKADPLIFRVNDPFVWPCLAELVKTMKKGGKAVCVSPSKLAAGEYGDKKLRIPPC
                     KSIIFEVELVGIK"
     gene            complement(4661..5368)
                     /locus_tag="P5243_00835"
     CDS             complement(4661..5368)
                     /locus_tag="P5243_00835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRS18018.1"
                     /translation="MQLFYSEHITPTTILLPEEESVHCTKVLRRSIGQTIQVIDGKGT
                     MYDAEIIEIHKKQTICSIKTSHNNFGVHPYFLRMCVAPTKNIDRFEFFIEKAVEMGVN
                     EIIPIQCENSERITVKPERLERICISAMKQSFKAQKPIIAPIQPCSTILSSKYEGVCC
                     IAHCSDSEHKKNLFSQITKGIPTTIIIGPEGDFSKQEIQLAQQHNWIEVHLGTSRLRT
                     ETAAIAAVHTVYVKHSE"
     gene            5536..>6099
                     /locus_tag="P5243_00840"
     CDS             5536..>6099
                     /locus_tag="P5243_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS18019.1"
                     /translation="MSQKYLYIETYGCQMNFNDSEVVAAVLEQDGYTYTEEIKQASLI
                     LLNTCSIREHAENKVFQRLNVIKKMRKQNSKLLIGVIGCMAERLRDQLFDNDVLADIV
                     AGPDSYRNLPELVRLASQSKHAIDVHLSESETYNDITPYRVGNNRISAFVSIMRGCNN
                     FCTYCIVPYTRGRERSREVDSILNEVRD"
BASE COUNT         2076 a          850 c         1116 g         2057 t
ORIGIN      
        1 aaaacctatt accaatacaa aacaaaaagc attagttgaa tttaaaggaa gaacgttatt
       61 agaatatgct attttaaaat taaaatatta tggtgtatct gatattatta taaatgttca
      121 tcatttttca aagcaaataa ttgactatgt taatattcat gattttggtg ttcgtataac
      181 tatatcagac gaatctgatg aattgcttga taccggcgga ggacttctta aagccaaaga
      241 tttttttaaa gatgcagatt cttttattgt gtacaatgtt gatgttataa gtacaattaa
      301 tttgttagat ttttataaat ttcatatatc aaataatgcg ttagtttcat tagcagtttg
      361 taaaagaaat acatcacgat atttattgtt tgataccagt aaacaattgt gtgcttggga
      421 aaacacacag tctcatgaaa tgcaaataat tcatgaacgt gaagaatatt ttcaattggc
      481 ttttagtgga attcaaatta ttagtactaa aattttcgat ttaatttcag aagaaggagc
      541 ttttccaatc atgcaattat atttaaggtt ggctcaacag cataaaattt tagcatataa
      601 tcattcacaa gatttttggc ttgatgttgg aaaatttgat aaattacaag atgttgaaaa
      661 acaaattgca aatcaaccgc tagaatatct ttcatgaaac aatatacatg ggtagaacct
      721 gaaatttcaa aacaggttga tgaaattgaa ttgtggagta aacagtttgg tattcatgct
      781 aagttagtcc cgcttttatg gaacagaggt attctaacat ttgagaatgc aaaaattttt
      841 tttacccctt ctacaagcaa tttgcacaat ccttttctta tggccgatat gcaaaaagct
      901 gttgataggc ttgcattggc tctcgataca aatcaaaaaa tattggttta tggtgattat
      961 gatgtagatg gaactaccgc tgttgctatg gtatattctt ttttgaaatc atgcaatgcg
     1021 caagttaaat attatgttcc cgacagatat accgaagggt atggcgtatc tattcaggga
     1081 atggattatg caattcaaca ttcgtgtaat cttatcatta cattagattg tggaataaaa
     1141 gcacatagag caattgaata tgttacacaa gcaggtatag acgtaatagt atgtgatcat
     1201 catgaaccgg cagaaacatt gcctccggca tatgcagtat tagatcctaa acgtgacgat
     1261 tgtaattatc catttaagga attatcgggg tgtggagttg attataaatt tttatatgca
     1321 ctttgtgaac aacgctcaat ttcaattaaa gagaatttga attgttatat ggatttattg
     1381 gctataagca tagcttcgga tattgttccc atagttgatg aaaatagggt tcttatatat
     1441 tttgggttac ttaaaataat gcgtaaacca catttaggta ttaaggcaat tacggaaact
     1501 tcgggcatat ttggtccaat tacagtgagt gatgtggtgt ttaaaatagg gccacgtata
     1561 aatgctgccg gacgaatgta taatgctaaa acggtaattg aattattgat ttccgataat
     1621 tatgaaaaag ctgtagatat ttgcaaacaa atacaagaat ataatatatc gcgacgtgct
     1681 attgataaat caattactga tgaggctata gctatgttgg aaaatgatga gcaaactgct
     1741 ttcaaaaaaa caaatgtgtt atataaacca agttggcata aaggggtaat aggaattgtt
     1801 gcatcgcgtg tgcttgagca attttataaa cctaccgtta tattttgtgg tgaaggtgat
     1861 attatttcag cttcggctcg ttcggttgct gaatttgatt tgtataaagc gttagaacaa
     1921 tgttcagatt tacttgtaaa ttacggtggg catacatttg cagcaggcat gtctatggaa
     1981 aaacaatatt taccgcaatt tattgagcgt tttgaaacta tagtttctca atctattaca
     2041 gaagacgcat tacaacctaa aatattgatt gatgctgagt tgaatattga tgatatcact
     2101 ctgcaattgt atgaagatat gatgcgtttt gctccttttg gtccacagaa tatgactccg
     2161 gtttttatga ttagaggatt atgtgataca ggaaaatcga aaataatggg gaacgaccaa
     2221 cgtcatgtaa aacttgagtt gatgagttct ggtaaatcta caaaaagtat tgaagcaatt
     2281 ggttttggac tagctcatac atggaaacaa atttcaaaaa ctcatagtaa tattgatatt
     2341 tgttttacaa tacgagaaca tgaatttaga aatattaagt cattacaatt agaattacgt
     2401 gatattagac cacatataga ttattcacag gtgtaatagt atttatattc atctaaatat
     2461 tcaggttgca acgattctat actatctaat tcagtcccgc attttgttcc ttccgaagat
     2521 tttgcttcaa tggctccgtt tgtagtattt tcttttacac tatagcactg agcacattca
     2581 tttgccggat ctacacatgc acttattgtt ataagaaatg gtattacaat aaatagtata
     2641 ttttttttca tacaagtata tattatgaat tttatagtgt aaatgtacta aaatcaatta
     2701 aaatttatca tttttgcata aaatttattg ctatgaagaa aacaattttg ataaccgggg
     2761 gggcaggttt tattggttcg catgtagtta gaaggtttgt tacacaatat cctgagtata
     2821 ctattgttaa tcttgacttg ttaacctacg caggaaatct tgaaaattta aaagatattg
     2881 aaaataaacc aaattatata tttgaacgtg gagatatagc cgatgttgaa aaagttcatc
     2941 agttatttga aacatacaaa tttgatggag taattcatct tgctgcagaa tctcatgtag
     3001 atcgttcaat tacagaccca ttttcatttg ttagaaccaa tgtatatggt accgtagtgt
     3061 tgttgaatga agcaaaaaat gcatggaaag ataattatga cggtaagcgt ttttatcatg
     3121 tttcaaccga cgaagtgtat ggttcgttgc acgatggcgg tttttttgtt gaaacaacac
     3181 cgtatgaccc acaaagtcca tattcggctg ctaaagcaag ttctgaccat tttgtaagag
     3241 cctatgcaaa tacctataaa ttacctattg ttataagtaa ttgttcaaat aattatgggc
     3301 caaatcattt cccggaaaag ttaataccat tgtgtattaa taatattatg aataaaaaac
     3361 cacttccaat atatggtaaa ggagacaatg ttcgtgattg gttgtatgta atagaccatg
     3421 caattgcaat tgatttgatt tatcacaaag ctgaagcggg agaaacatac aatattggag
     3481 gaaacaatga gtggacaaat cttaatttag taaaaaaatt atgtgaaata atggatacta
     3541 aattaggtcg tacacccgga gaatcggctc aacttataac atttgtaaaa gatagagcag
     3601 gacacgattt acgatatgct atagattctt caaaacttat gactaaactt gggtggaaac
     3661 catcggttac atttgaacaa gggcttgata taacagtaga ttggtatttg caaaatcaag
     3721 aatggctgcg taatgtagtt tctggtaatt atgaaatgta ttacgaaaca atgtacaaac
     3781 aacgatagat atgaggaatt ggatttttta tggcttgatt atagtatttt tttgggcatg
     3841 ttcgtcagaa aattccaaaa aatacgaatc tacagaaaat ggtttagaat ataagtattg
     3901 tgtaaaatct gaatcgaata agaaacctca atctggtgat aaactgttgt taaaagtgag
     3961 gttctataac gaaaaagata cgttattatg ggattctcga gagatctcat ctcgttttat
     4021 tatggatttt gtgcagcctc aatcacccgg taatactgtt aacgatgctt atgcaatgat
     4081 gcacgaaggc gatagtatga gttttttaat taatgctcgt caattttatt ccgctgccga
     4141 tccaaaatct ccaatgattc aaaaatttag agatgatgaa aaacttcgat tccaaataaa
     4201 attagaaaaa atatattctc tttcggaatt tgaagaagaa gcaaaaaaag taataaaacc
     4261 attgtgcgaa gaggagaaaa tgttgcttga agattatatt gaaaaacaat atcctgaagc
     4321 aaaacctact caatcggggt tatatataat tcacacaaaa caaggtgccg ggaaacaggt
     4381 aaataatcaa gatgaagtag ctattcatta tagagcaacc tatattaatg gagaacttat
     4441 ttatacaact tatgctaaag cagacccatt aatttttaga gtaaatgatc cgtttgtgtg
     4501 gccgtgttta gccgaattag taaaaactat gaaaaaaggt ggtaaggctg tatgtgtatc
     4561 accttcgaaa ttagcagccg gtgaatatgg tgacaaaaaa ttacgaattc ctccctgcaa
     4621 atcaataata tttgaagttg aattggttgg gataaaataa ttattctgaa tgttttacat
     4681 atacagtatg aactgcagct atagctgcag tttcggttcg taaacgactt gttcctaaat
     4741 gtacttcaat ccaattatgt tgttgtgcca attgaatttc ttgtttacta aaatcacctt
     4801 cgggaccaat aatgatagtt gttggtattc ctttggttat ttgagaaaat aaattttttt
     4861 tatgctcact gtcactgcaa tgtgctatgc aacaaacacc ttcgtattta ctcgaaagta
     4921 tggtgctaca tggttgaata ggagcaatga ttggtttctg agctttgaat gattgtttca
     4981 tagccgatat gcaaatgcgt tctaatcgtt caggtttaac tgtaatgcgt tcagaattct
     5041 cacattgtat aggaattatt tcatttactc ccatttccac tgctttttct ataaaaaact
     5101 caaatcgatc tatgtttttt gtgggagcaa cgcacatgcg aagaaaatag gggtgaacac
     5161 caaaattgtt atgagaagtt ttaattgaac aaattgtttg ctttttgtga atttctatta
     5221 tttcagcatc gtacatagtt cctttaccat caattacttg tatggtttgc ccaatgctac
     5281 gtcgcaatac tttggtgcaa tgcacacttt cttcttcagg taaaagaatt gtagtagggg
     5341 taatatgttc ggaatagaat aattgcatat tattacaatg aatacaaata tatatttatt
     5401 aggaacaata tgcagtatat ttttttgatt tcttttcaat tttgctttta ggtacgatat
     5461 taatcagtga aaaattaaat ggtaataatt aaaaattaat aattaacatc tatttttgcc
     5521 atcaaaatta ctactatgtc tcaaaaatat ctctatatag aaacgtatgg ttgccagatg
     5581 aattttaatg atagcgaggt tgtggcagcc gtgctcgaac aagatggata tacatatacc
     5641 gaagaaataa aacaagcatc gcttatttta ttaaatactt gttctattcg tgaacatgcc
     5701 gaaaataaag tttttcagcg tttgaatgtg attaaaaaaa tgcgtaaaca aaactctaaa
     5761 cttcttatag gtgtaatagg gtgtatggca gaacgacttc gcgatcaatt gtttgataat
     5821 gatgtattgg ctgatattgt tgccgggccc gattcgtata gaaatttacc cgaattggtt
     5881 cgtttagcat cgcaaagtaa acatgccata gatgtacatt tgagtgaaag tgagacatat
     5941 aatgatataa ctccttacag agttggtaat aatagaattt cggcatttgt ttctattatg
     6001 cgtgggtgta ataatttttg tacttactgc attgttccct atactcgtgg gcgtgaacgg
     6061 agtcgtgaag tagattctat actcaacgaa gttcgtgat
//
LOCUS       DAOTOW010000027         6096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11067_length_6096_cov_3.709154, whole genome shotgun sequence.
ACCESSION   DAOTOW010000027 DAOTOW010000000
VERSION     DAOTOW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11067_length_6096_cov_3.709154"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="P5243_00845"
     CDS             <1..389
                     /locus_tag="P5243_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS18020.1"
                     /translation="KKEDKQKVVWAVYLNKYFKSKISLLVQKSNDSNLKKQIHSNVVF
                     IKSVLDQNKIEYETIEAEDKKDFSDQVIDYAEKINANAILITTSRKLDIADYMFGATE
                     QKIIANKPCISVITVFPREGKLQGFN"
     gene            440..2095
                     /locus_tag="P5243_00850"
     CDS             440..2095
                     /locus_tag="P5243_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS18021.1"
                     /translation="MRYTVILFFCFVYTFVVAQSPGRYRISFTDKNNSVYSIQRPQEF
                     LSQKAIERRQKFAIEITMEDIPVNSWYIDSVLHYNTKLCNISKWFNSAVFVCDSTTNF
                     AAIKALPFVKSIEKVAPFHKAQPDATPLKTENKNDISYLEFYTNYKEELYGNSIRQIG
                     LMNGVKLHTDGFMGQGMTIAVLDAGFLHVDSATAFSQAWKENRILAYRDFSGAGVDVW
                     KEGYHGMAVLSLIAAYLPKTMIGTAPRANYVLCRTEEGATEYRVEEDNWVAAAEFADS
                     IGVDVISTSLGYSEFDDSTQNYTYKQMDGKTTRITVASNIAASKGMLLFNSAGNSGDK
                     PWKYITAPADSDSIITVGACYLNGKVTSFSSRGPSNGSTLKPDVVALGFYPAILKSNG
                     LVQVSGMGTSFSNPILAGMATCLWQEFPNLPPRTIKQAIIASSNRFFAPDTIYGHGIP
                     DFENARFILRMGMLQKTFDKAEVYPNPFRQILTVRIYSSKSSFVSIQMFDVQGKELLN
                     KRFEMSSLLHEIEIPEAASLPSGMYTVFVNFDKEQQIIKVLKK"
     gene            complement(2162..3016)
                     /locus_tag="P5243_00855"
     CDS             complement(2162..3016)
                     /locus_tag="P5243_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18022.1"
                     /translation="MKRILIIIASMCLSLVVFAQRPTAKKAQVANFENFTLYVVLNQN
                     PTSGYNEMIREAVRKSWRVSKYDFITQSEFDSKRTEENSAFLITNEIRFPKDKNVVPY
                     DFISIVLGGNFASYQVMPEVCTFPLMYTDSDEDNMLYKLPVIIGFFNQHIKNILVNPK
                     LLKDKKYTYYTKQKLSIAKKTLYLIAKEQLPEFDNAQKIRNVFKGKVVMIGQEEMANL
                     IKKRATNVVFLHKVGPADNSMQGSRCYIIMMGTDNTLYYFNYHILSAKAPNGISAKYW
                     KIFSSSVN"
     gene            complement(3044..4441)
                     /locus_tag="P5243_00860"
     CDS             complement(3044..4441)
                     /locus_tag="P5243_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS18023.1"
                     /translation="MNYFWRIITIVLISCLLQPSAYSQEYINVPRATQLTRILFIFDG
                     SMSMIGMWEQRSKIDVARSVLLPFLDSIAKIPNIEMALRVYGNRSPVPPQDCNDTHLE
                     VPFGKNNVPEIMKIILDMKPKGTTPIARSLELAANDFPKDSAARNIIFLITDGIEACD
                     GDPCAISRELQKKGAVLKPFIIGVGTDINFEEVFKCAGNVFSAKTELEFLPILHTAME
                     KALVTTPLQVYLLDAYKKPRETDVPMTFYDNSNGFIRYNFVHSVIKPAEPDILFIDPL
                     VTYKIKVHTMPPVFSDTVVLEPGKHTIVRVNVPQGYLVIERPFGMSTFTNLQAIVRKA
                     DNMNTLNTQYVNEKCKYLIGKYDLEIFTLPRTYYYGVEIKPDEVTTIKLPAPGRVSFN
                     RTQQGYGTIYIDKNTDLEFVANLDVVPKGNDSFLLQPGKYVVVWREKKETDTEKSMYV
                     NFEISSGSSKFIQLK"
     gene            4636..5937
                     /locus_tag="P5243_00865"
     CDS             4636..5937
                     /locus_tag="P5243_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRS18024.1"
                     /translation="MAQRTLHFDTLQVHAGHTPDSDTNSRAVPLYQTTAYTFNSAEHG
                     ANLFALKEFGNIYTRLMNPTTDVLEKRIAALEGGIAALAVASGHSAQLIAITNIMGQG
                     SNIVSSSHLYGGTYNQFKVTFPRFGITTKFVQTQRVEDYEALIDENTRAIYAETIGNP
                     EFSVPDIEGLAKIAEKHGIPLIIDNTFGAGGYICKPIQWGAHIVTTSATKWIGGHGTS
                     MGGVIIDSGSFNWGNGKFPFFTEPSEGYHGLKFWEVFGSHSPFGNIAFIIRARVEGLR
                     DLGPAISPFNSFQLLQGIETLSLRMQKTVDNTLALAQWLEKHPKIEHVNYPGLASSKY
                     HTIAKKYLQHGFGGVLSIRVKGGKEAAEQVINNLQLASHLANVGDAKTLVIQPAVTTH
                     QQLSEQEQIAAGVFPNLIRISVGIEHIDDIIWDFEQALEQI"
BASE COUNT         2076 a         1036 c          955 g         2029 t
ORIGIN      
        1 ataaaaaaga ggataaacaa aaagttgtat gggcagtata tttgaataaa tattttaaat
       61 caaaaatttc tttactcgtt caaaaatcta atgattctaa tttgaaaaaa caaattcatt
      121 caaatgtagt attcataaaa tctgttttgg accaaaacaa aattgaatat gaaacaatag
      181 aagctgagga taaaaaagat ttctctgatc aagtaattga ctatgcagaa aaaataaatg
      241 caaatgctat acttataaca acatctcgta aactagatat tgcagactat atgtttggcg
      301 ctacggaaca aaaaattatt gctaataaac catgtatttc tgttattaca gtattcccac
      361 gcgaaggcaa attgcaaggg tttaattaat tctctattat ttattttata gattagaata
      421 gattgacgtt tttttttgaa tgagatatac tgtaatttta tttttttgtt ttgtatatac
      481 ttttgttgta gctcaaagtc ccggtagata tagaatttca tttaccgaca aaaataattc
      541 tgtatattct attcaacgcc ctcaagaatt tcttagtcaa aaagctattg aacgacgaca
      601 aaagtttgca attgaaataa caatggaaga tatacccgtg aattcatggt atattgatag
      661 tgtattgcat tacaacacta aattatgcaa catttctaag tggtttaatt cagcagtatt
      721 tgtttgtgat tctactacta attttgctgc tattaaggct cttccttttg taaaatctat
      781 tgaaaaagtt gctccgtttc ataaagccca acctgatgca actcctttaa aaacagagaa
      841 taaaaatgat atctcatatc ttgaatttta tactaattat aaagaagaat tatacggaaa
      901 ttctattaga caaattggtt taatgaatgg ggtaaaactg cataccgatg gtttcatggg
      961 gcaaggtatg actatagctg ttcttgatgc gggattttta catgtagatt cagctacagc
     1021 attttcacaa gcatggaaag aaaacagaat ccttgcatat agagattttt caggagcagg
     1081 agttgatgtg tggaaagaag gatatcatgg tatggctgtt ttatcactta ttgcagcata
     1141 tttgccaaaa acaatgatag gcaccgctcc tcgagctaat tatgtattat gccgtaccga
     1201 agaaggcgcc acagaatatc gcgtagaaga agataattgg gttgcagctg cagaatttgc
     1261 tgacagtatt ggagtagatg taatttcaac atcattaggg tacagtgaat ttgatgactc
     1321 tacgcaaaac tatacttaca aacaaatgga cggtaaaaca acgcgtataa ccgttgcaag
     1381 taacatagcc gcatctaaag gcatgttgtt atttaacagt gccggaaatt caggagacaa
     1441 accatggaaa tatattacag cacctgctga ttctgatagt attataacag taggagcttg
     1501 ttatttaaac ggtaaagtta catcattcag ttcacgaggt ccttcaaatg gttctacatt
     1561 aaaacccgat gtagtagctc tcggatttta tcccgcaata ctcaaaagca atggattagt
     1621 tcaagtttcg ggtatgggca catcattttc taatccaatt ttggcaggta tggctacctg
     1681 cctatggcaa gaatttccaa atcttcctcc tcgcactatt aaacaagcaa ttatagcatc
     1741 gtcaaataga ttttttgcac ctgataccat ttatggacat ggtattcctg attttgaaaa
     1801 tgcacgattt attttacgta tgggtatgct gcaaaaaaca tttgataaag cagaagtata
     1861 tccaaatccc tttagacaaa tattaaccgt tcgaatatat tcgtcaaaaa gttcttttgt
     1921 atctattcaa atgtttgatg tgcaaggtaa agaattgttg aataaacgat ttgagatgag
     1981 ttctttatta catgaaattg aaatacctga agcagcttcg cttccgtcgg gtatgtatac
     2041 tgtatttgtg aattttgata aagaacaaca aataataaag gtattaaaaa aataaatcca
     2101 atacctttac ataaaactta tctaaattgt ataatttttt cttccgaatt tttgtacaat
     2161 tttagtttac acttgaagaa aatatcttcc aatattttgc cgaaatacca tttggtgctt
     2221 ttgctgacaa tatgtgataa ttaaaataat acaacgtatt atcggttccc atcattatta
     2281 tataacaacg tgaaccttgc atagaattat ctgcaggtcc tactttatgt aaaaatacaa
     2341 cattagtagc acgtttttta attaagtttg ccatctcttc ttgccctatc attaccactt
     2401 tacctttaaa aacattacga attttttgag cattatcaaa ttcgggtaat tgctctttgg
     2461 ctattaaata tagcgttttt ttagcaatac ttaatttttg ttttgtgtaa tatgtatact
     2521 ttttatcttt caataattta ggattaacca aaatattttt aatatgctga ttaaaaaagc
     2581 caataattac aggtaactta tagagcatat tatcttcgtc actatcggta tacataagag
     2641 ggaaagtaca aacttctggc attacctgat agcttgcaaa attacctccc aacacaatac
     2701 ttataaaatc atacggaact acatttttat ctttaggaaa acgaatttca ttagttataa
     2761 gaaatgccga attttcttcg gttcgttttg aatcaaattc cgattgtgta ataaaatcat
     2821 attttgaaac tcgccaactt ttgcgaactg cttcacgaat catttcattg tatccactag
     2881 taggattctg attgagtacc acatataacg taaaattttc aaaattggct acctgagctt
     2941 tttttgcggt tggtctttgt gcaaaaacaa ctaaagataa gcacatactt gcaataataa
     3001 tcaatatcct tttcataaca ttatagtttt aattaaacgt cttttatttt aattggataa
     3061 atttcgaact tcccgacgaa atttcaaaat ttacatacat agatttttct gtatctgttt
     3121 cttttttttc acgccacaca actacatatt taccgggttg caacaaaaat gaatcatttc
     3181 ctttaggaac cacatctaaa tttgccacaa actccaaatc ggtattttta tcaatataaa
     3241 tagttccata gccttgttgt gtcctattaa aactcacacg tcccggagca ggtaatttaa
     3301 tagtagtaac ctcatcaggt ttaatttcca ctccataata atacgtgcgt ggtaatgtaa
     3361 atatttctaa atcatatttt cctattaaat atttacattt ttcatttaca tattgtgtgt
     3421 ttaacgtatt catattatca gctttccgta caatggcctg caaatttgta aacgttgaca
     3481 taccaaacgg acgttctata actaaatacc cctgcggcac atttacacga actatagtat
     3541 gtttgcctgg ctctaataca actgtatcag aaaaaaccgg aggcatggta tgaactttta
     3601 ttttataggt aaccaatggg tcaataaaca aaatatcggg ttcggcaggt tttattaccg
     3661 aatgcacaaa attataccga ataaacccat ttgaattatc gtagaaagtc ataggcacat
     3721 cggtttctct cggttttttg taagcatcta ataaatacac ttgcaacggc gtagtcacaa
     3781 gagctttttc cattgctgta tgcaaaatag gtaaaaattc caattctgtt tttgcactaa
     3841 atacatttcc tgcacattta aatacttctt caaaatttat atctgttcct accccaataa
     3901 taaatggttt taaaacagct cctttttttt gtaattcacg tgaaattgca caaggatcac
     3961 catcgcaagc ctcaatacca tctgtaatta aaaaaattat attacgagct gccgaatctt
     4021 taggaaaatc attagccgct aactccaacg aacgtgcaat aggagtagta cctttgggtt
     4081 tcatgtccaa aattattttc ataatttccg gcacattgtt tttcccaaac ggcacttcta
     4141 aatgagtatc attacaatct tgaggaggca ccggactacg attcccataa acccgtaatg
     4201 ccatttctat atttggtatt tttgctatac tatctaaaaa cggaagcaaa acacttcgtg
     4261 caacatctat ttttgaacgt tgttcccaca ttcctatcat actcatacta ccatcaaaaa
     4321 taaataatat acgcgtaagc tgcgttgctc gcggcacatt aatatattct tgtgaatatg
     4381 cacttggctg cagtaaacaa cttattaata ctattgtaat aattcgccaa aaataattca
     4441 tactttatgc tttattgcaa atatataaaa ataccgaact tatactatat ataatagctt
     4501 aaaaacaaag aatagtatat aagaccaaat atttttttac atttgatgta aaaattattt
     4561 gacttttaaa aattataccg tatatttgta tcctactaaa acaataggat taatagtata
     4621 aaatattttt acgatatggc acaacgaaca ttacactttg acacattaca agttcatgca
     4681 ggacatactc ccgacagtga cacaaattca cgagcagttc ctttatacca aacaacagca
     4741 tacacattta attctgctga gcatggagct aatttatttg ctcttaaaga gtttggaaat
     4801 atttatacac gcttaatgaa tccaacaaca gatgttcttg agaaacgtat agctgcttta
     4861 gaaggtggta ttgccgcact tgcagtagca tcaggacatt ctgctcaact tatagctatt
     4921 accaatatca tgggacaagg ttccaatatt gtaagcagct cgcatttata tggtggtaca
     4981 tacaatcaat ttaaagttac atttcctcgg tttggtatta ccactaaatt tgtacaaaca
     5041 cagcgcgtag aagactacga agctctcatt gatgaaaata ctcgtgctat ttatgcagaa
     5101 actattggca atccggaatt tagtgtgccg gatattgaag gtttagcaaa aattgccgaa
     5161 aaacatggta ttccattgat aattgacaat acatttggtg ccggtggata tatttgcaaa
     5221 cctatacaat ggggagctca tattgttact actagtgcta ccaaatggat tggcggacat
     5281 ggtacaagta tggggggagt tattatagac agtggttctt ttaattgggg taatggtaaa
     5341 tttccatttt ttacagaacc atcagaaggg taccacggac tgaaattctg ggaagtattt
     5401 ggttctcata gtccatttgg taatattgct tttattattc gtgcccgtgt agaaggatta
     5461 cgagatttag gtccagcaat cagcccgttt aattcttttc aattattaca aggtattgaa
     5521 acgctctcac tacgtatgca aaaaactgtt gacaacacat tagcgctagc tcaatggtta
     5581 gaaaaacatc ctaaaattga acatgtaaac tatcccggat tagcttcatc aaaatatcat
     5641 acaattgcta aaaaatattt acaacatggt tttggcggag tattaagcat acgtgtaaaa
     5701 ggaggaaaag aagctgctga acaagtaatt aataacttac agcttgcaag tcaccttgca
     5761 aatgtaggcg atgcaaaaac tctggttata caacctgcag taactactca tcaacaatta
     5821 agtgaacaag aacaaatagc agcaggagta ttcccaaatt taattagaat atcggttggt
     5881 attgaacata ttgatgatat tatttgggat tttgaacaag cattagagca aatataattt
     5941 cacacaaaat tatatttgct gctttattta tcttcgctta ctgtaaacta tgcttataat
     6001 acaagccgct atgagtatat ataatgcata tgtgtaaata taatacccta caatagcata
     6061 tttgggttca tatgtaaaag ttacttccac tttacc
//
LOCUS       DAOTOW010000028         6087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11087_length_6087_cov_3.572281, whole genome shotgun sequence.
ACCESSION   DAOTOW010000028 DAOTOW010000000
VERSION     DAOTOW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11087_length_6087_cov_3.572281"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="P5243_00870"
     CDS             complement(<1..769)
                     /locus_tag="P5243_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRS18025.1"
                     /translation="MNIDATIFSRISSLVEQYTVITIVTHINPDGDAIGSTLALAGIL
                     VQLDKRVTVVTPNDCPENLQWIQGYSSILKYDKQKQEVLQCIAQTELFFCLDFNNISR
                     LEELGENIAKSSAPHILIDHHPQPECFASVMLSVTEASSTSELVYHTIMGCGYEKYLN
                     TQVAESLYTGIITDTGGLSHNSGRPELYRIIAHLLEKGIDKTAIHDVIFNVFSYDRMK
                     LLGTILKDNFVYLPEYKTAYMYITIENQKNHNFQLGDS"
     gene            complement(775..1611)
                     /locus_tag="P5243_00875"
     CDS             complement(775..1611)
                     /locus_tag="P5243_00875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS18026.1"
                     /translation="MVIILTFVSCSNSFNSYEGYTKHEKGYWYKLHSFEEGYIKAKNT
                     DFVSAEIQFSIPNNDSLVFTDSYIIQVSNTDSVCLPLMMHDVKQGDSISFIAPNTRCI
                     RNVLPAEFSDLFSDAKELQITAKILWVNDAITYEKKVKEYAIWLASKLEFEKNYIQTF
                     ISKHSLTFTLQNGIYKSIVKPGTGRIPQNGDIVSIRYQGSVLSGEIINHFTSLEFVYG
                     SQWQVIEGIEKALATMKQGERAVIIVPSEFAWGEKGTSDGSIPPFTTVQFDLELVSVT
                     QK"
     gene            complement(1674..3512)
                     /gene="yidC"
                     /locus_tag="P5243_00880"
     CDS             complement(1674..3512)
                     /gene="yidC"
                     /locus_tag="P5243_00880"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRS18027.1"
                     /translation="MDRNTIIGTILIALFIIAYFFITKPSPEEIAKQQRYIDSVQQVE
                     YKKREIARQTEDSLAMLQQQVQATELNMPDSLKQLAAQKEFGRYAAAAVGTEQFDVLK
                     NNKIEVTFSNKGGKPYRVHVNEYKTYYQKPLYLLSGDSTKFGLRLSEIGKSTNSLFFT
                     KVSQTNSSDSVQTITYRLYAGQYAYIDYIYKLEPDSYAVQMLIQTVGMDQYIKESRLS
                     LYWETYMNSFEKSRKIEGQNTNLVWKYYEDDVESLNAHASEPKKEDITGKVKWVGFKY
                     QYFSAIIIAKENFERGGQVVSKPLSENDSSLKILSAELFMPNQKSNELTFYFGPNHYP
                     TLKKQGAEIEGILDLGWFGFITKFAIIPIFNWLNSFLTNFGLIILLLTIILKISLFPL
                     TFKSYISTARMRVLKPQTDEIAAKYPNPADAMKRQQATMEMYRKAGVNPMGGCLPMLI
                     QFPILIAMFRFFPASIELRQESFLWAEDLSSYDSILSLPFSIPFYGDHVSLFTLLMAV
                     SMIFVNQLNMSNTTTTPGMPNMKVMMWIMSIMMVFWFNSYSSGLSYYYFLANVITLLQ
                     TFIIRQMIDDKKILAKIEANKKNPKKKSSFQERLEKMAREQQRLRK"
     gene            complement(3594..5210)
                     /locus_tag="P5243_00885"
     CDS             complement(3594..5210)
                     /locus_tag="P5243_00885"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRS18028.1"
                     /translation="MSKTKFIFVTGGVASSLGKGIISASIGKLLQARGYKVTIQKLDP
                     YLNIDPGTLNPYEHGECYVTIDGAETDLDLGHYERFLNTPTTQANNVTTGRIYKNVIE
                     KERRGDFLGKTVQIIPHITDEIKRNIKLVSIKGEFDFVITEIGGTVGDIESLPFIEAV
                     RQMKWEHEGRCLFVHLTFVPFLAASGELKTKPTQHSVKHLLEAGIQPDVLVLRTEKPL
                     SKDLKKKVGLFCNVDIDSVIEARDMATIYEVPLAMLEEKLDVTILKKFKMKVGEQPEL
                     LPWRTFIEKHKNPKQTLQIALVGKYVELADAYKSIIESFVHAGASLECKVKIKLVHSE
                     ELTDDNVAHYLQDCMGVLVAPGFGHRGIEGKFTAIKYAREQLVPFFGICLGMQCAVIE
                     FARNVLGYKQANSTEMSRTTPFPVIDIMQEQKNVSDMGGTMRLGGYPCTLKKRSNVHK
                     IYQADTIIERHRHRYEFNNKFLDEFEKAGMIATGINPDTNLVEIVEIPQHPWFIGVQF
                     HPEYRSTVIAPHPLFQSFVKAAIVYSKHKK"
     gene            complement(5238..>6087)
                     /locus_tag="P5243_00890"
     CDS             complement(5238..>6087)
                     /locus_tag="P5243_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18029.1"
                     /translation="NILLYIVSVIVLLSCEAKQSDSSQSFSNKTKDASVAEELKRLGL
                     PEKLDFLIDNLVLSDNMIMTLSSDTTIYNATLLNNNENAKLYTTSRSKAVNLGVYGAD
                     LNYLIHFGQSQFSIKYMVVSKQLADQIGVAMAFDQETMDEYQSNVENKDSLINIIFLA
                     YDNVKKMLKSEDQFLLSTLVIVGSWIENMYITTELLPYFKSRQAKAKLATKIVQQHDY
                     LQKMIELLSNLNEGDNMYVNEILQDLKKIDAIYVSFGDKLLVEDDIKLLNTQIEEMRL
                     KIIKVQ"
BASE COUNT         1989 a         1082 c          850 g         2166 t
ORIGIN      
        1 cagaatcacc taattgaaaa ttatgatttt tttgattttc aatggttata tacatatatg
       61 ccgttttata ttcgggcaaa tacacaaaat tatctttaag aatagttccc aacaatttca
      121 ttctatcgta tgaaaataca ttaaaaatga catcgtgaat tgctgtttta tcaattcctt
      181 tttctaataa atgagctata atacgatata attccggacg acctgaatta tgcgaaagac
      241 caccggtatc tgtaataata ccggtatata acgattcggc aacttgtgtg tttaaatact
      301 tttcgtaacc acagcccatt attgtgtgat ataccaattc tgatgtagaa ctagcttcgg
      361 taacagacaa cataacagat gcaaaacatt ctggttgagg atgatgatct ataagtatat
      421 gaggagctga acttttagca atattttctc ctaattcttc taatcgtgaa atattattga
      481 aatctaaaca aaagaataat tctgtttgtg caatacattg taatacttct tgtttttgtt
      541 tatcatattt taaaatagaa gaatatccct gaatccattg taaattttcg ggacaatcat
      601 ttggtgtaac tacagttaca cgtttatcta attgaaccag aattcctgct aatgctaaag
      661 ttgaacctat tgcatcacca tcgggattaa tatgtgttac aatagttatt acagtatatt
      721 gctcaaccaa agaactaata cgtgaaaaaa ttgttgcatc aatattcata agtattattt
      781 ttgagtaacc gaaactaatt ctaaatcaaa ttgtactgta gtaaagggag gtatagaacc
      841 atcacttgta cctttttcac cccatgcaaa ttcgcttggc acaataatta ctgctctttc
      901 tccttgtttc attgttgcta atgctttctc tattccctct attacttgcc attgtgaacc
      961 atatacaaac tcaagtgatg taaaatgatt gatgatttcg ccactcaaca cactcccctg
     1021 ataacgaata ctaacaatat cgccattttg aggaatcctt ccggtacccg gttttactat
     1081 tgatttgtat attccattct gtagagtaaa cgttaatgaa tgttttgata taaacgtttg
     1141 tatataattc ttttcaaatt caagtttact ggcaagccat attgcatact cttttacttt
     1201 tttttcatac gttattgcat cattaaccca caaaattttt gcggtaattt gtaattcttt
     1261 agcatcagaa aacaaatcag aaaattcggc aggcaacacg ttccttatac aacgtgtatt
     1321 tggtgcaata aaagaaatac tgtcaccttg ttttacatca tgcatcataa gcggcaaaca
     1381 tactgaatct gtattgctta cttgaattat atatgaatcg gtaaatacca atgaatcatt
     1441 attaggaata gaaaattgta tttcggccga aacaaaatct gtattttttg ccttaatata
     1501 gccttcttca aacgaatgaa gtttatacca atatcccttt tcatgcttag tatacccctc
     1561 gtatgaatta aaagaattac tacatgacac aaaagtcaga ataattacca ttatttgtac
     1621 aaatactctc atatcaaaaa aaagccttac acaaaaaggt aaggcttata tttttatttt
     1681 cgtaatcgtt gttgctcacg tgccattttt tcaagtcgtt cttgaaaact tgatttcttt
     1741 ttaggattct ttttatttgc ttcaattttt gccaaaattt tcttatcatc aatcatttgt
     1801 cgtataataa acgtttggag caatgtaata acatttgcaa ggaaataata ataacttaaa
     1861 cccgatgagt aactattaaa ccaaaatacc atcattattg acataatcca catcattact
     1921 ttcatgtttg gcattcccgg agtagtagtg gtattcgaca tattgagttg attaacaaaa
     1981 atcattgaaa cagccataag taaggtaaat aaacttacat ggtcgccata aaatggaata
     2041 gaaaacggta aactcaaaat tgaatcataa cttgataaat cttcggccca caagaaactt
     2101 tcttgacgaa gttcaataga tgcaggaaag aaacggaaca ttgcaattaa aataggaaat
     2161 tgaatcaaca taggcaaaca accgcccata ggatttacac cggctttacg atacatttcc
     2221 atagtagcct gttgacgttt catagcatca gccggatttg gatatttagc cgcaatttca
     2281 tctgtttgcg gtttcaatac acgcatacga gctgttgaaa tatatgactt aaatgtaagc
     2341 gggaataatg atattttaag aattattgtt aatagtagaa ttattaatcc aaaattggtt
     2401 aaaaaactgt tcaaccaatt aaatattgga ataattgcaa actttgttat aaatccaaac
     2461 catcctaaat ctaaaattcc ttctatttcg gcaccttgtt tttttagcgt tggataatga
     2521 ttggggccga aataaaatgt taactcatta gacttttgat taggcataaa taattcggca
     2581 gataatattt ttaaagaact atcattttct gataatggtt tagaaacaac ttgtccgcca
     2641 cgctcaaaat tttctttagc tataataatt gcagaaaaat attgatattt aaaaccaacc
     2701 cacttaactt ttccggtaat atcttctttt ttgggttcac ttgcatgtgc atttaaactt
     2761 tcaacatcat cttcgtaata tttccaaact aaattagtat tttgcccctc aatttttctc
     2821 gatttttcaa aactattcat atacgtttcc caatacaaac tcaatcgtga ttctttaata
     2881 tattggtcca ttcctactgt ttgaataagc atctgaacag cataagaatc aggctcaagt
     2941 ttgtatatgt aatctatgta tgcatattga ccggcataca aacgatacgt tatagtttga
     3001 accgaatcag aagaattagt ttgcgatact tttgtaaaaa ataaactatt tgttgatttt
     3061 cctatttcac tcaaacgcaa cccaaatttt gtactatcac ccgagagcag atacaatggt
     3121 ttttgataat atgttttata ttcattcaca tgaactctat aaggtttacc tcctttatta
     3181 gaaaaagtaa cctctatttt attatttttc aaaacatcaa actgttcggt accaacagct
     3241 gctgcagcat atcgtccgaa ttccttttga gcagctaatt gcttcaaaga atcaggcata
     3301 tttaattctg tagcctgaac ttgttgttgc aacattgcta gtgaatcttc ggtttgacga
     3361 gcaatttcac gctttttgta ttctacttgc tgaacagaat caatatatcg ttgttgtttg
     3421 gctatttcct cgggactggg ttttgttata aaaaaataag ctattataaa taatgctata
     3481 agtatagtac caataattgt atttctatcc attgttgtat tattaaaaat tgaaaggcaa
     3541 aagtaagaaa aacttactaa tgcataatag gtatcacact tattttattt gaattatttt
     3601 ttatgttttg aatagacaat agcagctttt acaaacgatt gaaataacgg atgtggcgct
     3661 attaccgtac tacgatattc cggatgaaat tgaacaccga taaaccaagg atgttgaggt
     3721 atctcaacta tttcaactaa atttgtatca ggatttatac cggttgcaat catacctgct
     3781 ttttcaaatt catcaagaaa tttattatta aattcatatc tgtgtctatg acgctctatg
     3841 atagtatcgg cttgatatat tttatgtaca tttgaacgtt tttttaacgt gcacggatat
     3901 cctcccaaac gcattgtacc tcccatatct gacacatttt tctgctcttg cattatatct
     3961 attacaggga atggtgttgt tctactcatt tcggtagaat ttgcttgttt gtaccctaaa
     4021 acattgcgtg caaattcaat aacagcacat tgcattccta aacaaatacc aaaaaaaggc
     4081 accaactgtt ctcgagcata ttttatagca gtgaattttc cttctatacc acgatgccca
     4141 aatccgggag caacaagcac ccccatacaa tcttgtaaat aatgagcaac attgtcatcg
     4201 gttaattctt cggaatgtac cagtttaatt tttactttac attctaaact tgctcctgca
     4261 tgtacaaacg actcaattat agatttatat gcatctgcca attctacata ttttccaact
     4321 aatgctattt gtaatgtttg tttaggattt ttatgttttt caataaatgt tctccatggt
     4381 agcaattccg gttgttctcc aaccttcatt ttaaactttt tgagtatagt aacatcaagt
     4441 ttttcttcaa gcattgcaag cggcacttca taaatagttg ccatatcacg tgcttctatt
     4501 acagaatcaa tatcaacatt acaaaacaac cctacttttt tctttaaatc ttttgaaagt
     4561 ggtttttctg tacgcaacac aagcacatcg ggttgtatac cggcttcgag taaatgtttt
     4621 acagagtgtt gagttggttt tgttttaagt tcaccacttg cagccaaaaa tggaacaaat
     4681 gtcaaatgca caaataggca tctaccctcg tgctcccatt tcatttgacg aacagcttct
     4741 ataaacggta acgattctat atcaccaaca gttcctccaa tttcagtaat tacaaaatca
     4801 aattcacctt tgatacttac taattttata ttccgtttaa tttcatctgt tatatgagga
     4861 ataatttgaa ctgttttacc aagaaaatcg cctcgtcgtt ctttttcaat tacattttta
     4921 tatattctac cggtggtaac attattggcc tgtgttgtag gagtattcaa aaagcgttca
     4981 taatgcccta aatctaaatc tgtttcagca ccatctatag taacatagca ttcgccgtgt
     5041 tcatacggat tcaatgttcc cgggtcaata tttaaatatg gatctaattt ttgaatcgtt
     5101 actttatatc ctcgtgcttg taataatttt ccaatagaag ctgaaataat acctttacct
     5161 aatgacgacg caaccccacc tgttacaaat ataaactttg tcttactcac cgctttaata
     5221 aatttataaa aaaataatta ttgaacttta ataattttta accgcatttc ttctatttga
     5281 gtattgagca attttatatc atcttcaact aatagtttat cgccaaaact cacatatatc
     5341 gcatctattt tctttaaatc ttgtaaaatt tcattcacat acatattatc accttcattc
     5401 aaattgctca acaattctat cattttttgt aaataatcat gttgttgaac tattttggtt
     5461 gcaagctttg ctttggcctg acgtgatttg aaatatggta ataattcggt tgttatatac
     5521 atattttcta tccaactacc tactattaca agcgtagaaa gcaaaaattg atcttcactt
     5581 ttgagcattt ttttcacatt gtcatatgct aaaaaaatga tatttattaa agagtcttta
     5641 ttctcaacat ttgactgata ttcatccatt gtttcctgat caaaagccat tgctacacca
     5701 atttggtctg ccaattgttt agaaaccacc atatatttaa tagaaaattg tgattgacca
     5761 aaatgaatta aataattcaa atcggcgcca tatacaccta aatttactgc tttagaacga
     5821 ctggttgtat ataattttgc attttcattg ttgtttaaca aagttgcgtt ataaatagta
     5881 gtatctgacg acagtgtcat aatcatatta tcggaaagca ccaaattatc tatcaaaaaa
     5941 tctaattttt cgggcaatcc aagtcttttt aactcttcgg caacagacgc atcttttgtt
     6001 ttattggaaa aagattgaga agaatcggat tgttttgctt cgcacgaaag taaaacgata
     6061 acagatacaa tataaagtaa aatattt
//
LOCUS       DAOTOW010000029         6039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11205_length_6039_cov_2.860963, whole genome shotgun sequence.
ACCESSION   DAOTOW010000029 DAOTOW010000000
VERSION     DAOTOW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_6039_cov_2.860963"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="P5243_00895"
     CDS             complement(<1..613)
                     /locus_tag="P5243_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS18030.1"
                     /translation="MISYFQHKVVWITGASSGIGKETAIQLSRITHISLILSGRNETV
                     LKEVQSICVKNNAACSILLLDLEKTNELEEKAKEAIVLFGRVDIMIHNGGISQRSSVI
                     ETPIENHRKIMEIDYFSTIIITKTLLPHMIERGYGHIVAISSISGCFGFPLRSAYCAA
                     KHAMYGYYESLDIELRAQGVFVTIVAPGRIQTNISLSALTKDGT"
     gene            complement(619..2058)
                     /gene="dacB"
                     /locus_tag="P5243_00900"
     CDS             complement(619..2058)
                     /gene="dacB"
                     /locus_tag="P5243_00900"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRS18031.1"
                     /translation="MRFILTCGLIIITLGGFSQKLVQQKVESYCNTNPRLTYAQYAIS
                     VKDVKTDSVVVAINEKKSMTPASIVKLYTTSAALQELGKDFRFKTVVGYTGTIQKGVL
                     KGNLIIIGGGDPTLGSKFFPQSKQFVDSIVQAIKKKGIQSIDGLIVLDCSLYGKEAIP
                     QSWVWEDFGKDYATGVYPLSFYDNVFSITLKTGNVGDTAKITQVFPAEMSTTIQNNAI
                     VGDDKSQEPSILGNPEELSRTVSGVVPANRNSYIVRGAMPSPPDAFGMEIKQALFRAN
                     MVKYNQEYIVKYDPFFKDGMDTVLVLLSPELELIVGITNYHSNNSYAEHMLKYMGYAK
                     LNKGTFEAGTKVVQKHFTDLGMTSQGAMLYDGSGLSRYNTTTASHMTEFLIKKYQQGA
                     DTSTFFRSLPISGKPSTLRSYDFPSYMIGKINAKTGSMNRVRNMAGYMTTKSGKRVAF
                     CVMIQGFLDTDGDMKKTMLDILDSIYTNY"
     gene            complement(2080..3246)
                     /locus_tag="P5243_00905"
     CDS             complement(2080..3246)
                     /locus_tag="P5243_00905"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRS18032.1"
                     /translation="MRKILLFGISVLYSYCVYSQENTTNGFTPDVQLSYLYGSVKNPI
                     SIPNQGNAHYLQLSLLHQTGSQEQSLYALYKKPSIGATVLYGFLGSKDVLGNVIALYP
                     TWQYMFNTSKNLGASIRIGSGFAYFTNPYDKISNQDNKLVGSHISNCTEISPNIWFKF
                     APTTYVQTGVALMHFSNGHTAIPNIGLNDVTFRLGVIYRPGSLVGVAMPIRVKPNNDT
                     VWRKRIQLSVGRHELAYTTYPVDGPTYNIYKVGGYLTRQINRIHDFQLGLTMAFYDSY
                     YTFIKYENYYSHFKPFMSTQTTLHIGHEFLLQHFGILTEMGLKVLDPFYRSYFLADEI
                     DFAYTSKSLLSARVGLTYYPFNGAFASKKLGIGMFIKTNFVQADYVDYSISYTF"
     gene            3370..>6039
                     /locus_tag="P5243_00910"
     CDS             3370..>6039
                     /locus_tag="P5243_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRS18033.1"
                     /translation="MVPISLSLQGFYSYKDKQTIDFNSLTKAGVFGIFGQTGAGKSAI
                     IEAIMLALYGDIERINNREERSYNVMNLQSKELFIEFICFSHITQTKYTITVKAKRSS
                     KNFEQVSKLDRICYKLVDSKWIPINLEDIQKEITCLSYNNFKQTVIIPQGKFMEFLQL
                     SPTERTGMLKDLFNLHEYELSEKVKIVEGEIIQQKSRIEGNIQEIGEIDETVLATIQQ
                     KENELLTLGIEQKKQLDTLQENLKKLYLIKQDFDEFEKTKLIHKELLSKEQEFKELEK
                     TISLISLCVKYIAPIYDTLQLKQNDAGKLQSIITNAKQEFQKIEIRFNESKKIFEELE
                     QKFIQLPEFKQNTNFIKQCYELKNVEKQIETQTLKQQEFAKKIETLEKTQKTNEYSLQ
                     EQKELRKQLQQNVPDISSIYKQKHELEKYNTICNRIEECREKRIQYEHEIQTLKQQVC
                     DSAFTVFKQKIDYKDIKEIIEDKIKHCENIISHIDAELQQALLFNHVNTLRKTLQHGT
                     ACPVCGSTEHNIEKIASEYEPNIQDIEQKKIRISFELQSYTKLLQLLHTTNNHITVLQ
                     KQDHEISHTMHSLTIERNLQEPDFEIRDISVFEKELERITSIQQQMKNIENELIEFEK
                     KQKDIISELTIAHEQLIHIKTSHTETKTRFSMLKESIPSSLYNTYGSFDEDSLIQEIE
                     KRSKQQIEIETSYTSIKQEYILLKEQYNLLKISILEKEKSYKIICEDIQQLQEKLTFA
                     INTHSFDSTEQIENLLNQKESLLEYSQRLEHYKHALSTIETSVNTLSKKLEGVNFDTN
                     EFEQLKQTIEKAEHEIQITRDAYIRANEQRKQLQTKLQKQSDLLKQLAICKNDLDTIT
                     TLKSLFKQSGFVSYMSSNYIHMICKKANERFILL"
BASE COUNT         2299 a          982 c          786 g         1972 t
ORIGIN      
        1 gggtgccgtc ttttgtaagt gccgataatg atatatttgt ttgtattctc ccgggagcaa
       61 ctatagtaac aaatactcct tgagctctaa gttctatatc taaagattca taataaccat
      121 acatagcatg ttttgctgca caatatgctg aacgcaaagg aaaaccaaaa caaccggaaa
      181 tactacttat tgctacaata tgaccatagc ctcgttcaat catatgcggc aatagtgttt
      241 tagtaataat aattgtacta aaataatcta tttccataat ttttctatga ttttcaatag
      301 gtgtttcaat aacagaagaa cgttgactaa ttcctccatt atgaatcatt atatcaactc
      361 ggccaaatag tacaattgct tcttttgctt tttcttcgag ttcattagtt ttttctaaat
      421 caagcaataa tatactacaa gcagcattat ttttaacgca aatactctgc acttccttta
      481 atactgtttc attacgtcca gaaagtatta aagaaatatg agtaatgcgt gataactgaa
      541 ttgcggtctc ttttcctatt ccactcgaag caccggtaat ccaaacaacc ttatgttgaa
      601 aatacgaaat cacaaatttt agtaatttgt ataaatgctg tcaagtatgt cgagcatggt
      661 ttttttcata tcaccatcgg tatctaaaaa tccttgaatc ataacacaaa aagcaacacg
      721 ttttccggat ttggttgtca tatatcctgc catattacgt actcgattca tagaacctgt
      781 tttagcgttt atttttccaa tcatgtaact tggaaaatca tatgaacgta aagtacttgg
      841 ttttcctgag attggaaggc ttctaaaaaa tgttgatgta tctgcacctt gttgatattt
      901 ttttatcaaa aactcggtca tatgtgaagc tgttgttgta ttatatctag ataaaccact
      961 cccatcatac aacatagctc cttgggaagt catacctaaa tcagtaaaat gtttttgaac
     1021 tacttttgtt ccggcttcaa aagtaccttt atttagtttg gcatatccca tatattttaa
     1081 catatgttct gcataactat tattactatg ataattagtt atacctacaa ttaattctaa
     1141 ttcaggtgat aataaaacta aaacggtatc cattccatct ttgaaaaaag gatcatattt
     1201 tacaatatat tcctgattgt attttaccat atttgctctg aacaatgctt gtttgatttc
     1261 cattccaaaa gcatcaggtg gtgaaggcat tgcaccacga acaatatatg aatttctatt
     1321 ggcaggtact actcccgata cggttctgct taactcttcg ggattaccga gaattgatgg
     1381 ttcttgcgat ttatcatctc caactattgc attattttga atggttgttg acatttctgc
     1441 tgggaatact tgtgttattt ttgctgtatc tccaacattt cctgttttta gcgtaattga
     1501 aaatacatta tcataaaaac ttaaaggata cacaccggtg gcataatctt taccaaaatc
     1561 ttcccatacc catgattgag gaatagcttc ttttccatat aacgaacaat ccaatactat
     1621 aagaccatct attgactgta tacctttttt cttaatagct tgtacaattg aatcaacaaa
     1681 ttgtttactc tgtggaaaaa atttagaacc taaagtagga tctcctccgc caattataat
     1741 taaattccct tttaatacac ctttttgaat tgtaccggta tatccaacaa ctgttttgaa
     1801 tctaaaatct tttcctaatt cttgcaatgc tgccgaagtt gtatataatt ttacaataga
     1861 agcaggagtc attgattttt tttcattgat tgcaactaca actgaatctg ttttaacatc
     1921 ttttacactt attgcatact gtgcataggt aagacgtgga tttgtattac aatatgactc
     1981 aactttttgt tgaactaatt tctgactaaa tcctcctagg gttataatta ttaaaccaca
     2041 agttaaaata aatcgcataa atttcatcat aacaaatatt taaaaagtat atgaaataga
     2101 ataatcaaca taatcagctt gaacaaaatt ggttttaata aacataccaa ttcctaattt
     2161 tttagacgca aatgctccgt taaacggata atatgtaagc ccaacacgcg cagataaaag
     2221 actttttgat gtatatgcaa aatctatctc atctgcaagg aaatacgaac gataaaatgg
     2281 atctaatact ttgagcccca tttctgtaag tataccaaaa tgttgcaata aaaactcgtg
     2341 tcctatatgc aatgtagttt gagttgacat aaaaggttta aaatgtgaat aataattttc
     2401 atatttaata aacgtataat aactatcata gaatgccatt gtaagtccta attgaaaatc
     2461 atgaattcta ttgatttgac gagttagata tccaccaact ttataaatat tatatgttgg
     2521 accatctaca ggatatgttg tatatgccaa ctcatgtcta ccaacactta attgtattcg
     2581 ttttcgccaa acagtgtcat tatttggttt aactcgtatt ggcatagcaa caccaaccaa
     2641 tgaccccgga cgatatatta cccccaaacg aaacgttaca tcatttaaac caatatttgg
     2701 tattgcggta tgaccatttg aaaaatgcat aagagcaact cctgtttgga catacgtagt
     2761 tggtgcaaat ttgaaccata tatttggaga aatttcagta cagtttgata tatgagaacc
     2821 tactaattta ttatcctgat ttgaaatttt atcatatgga tttgtaaaat atgcaaatcc
     2881 tgatcctatg cgaatactcg cacctaaatt ttttgacgta ttaaacatat attgccatgt
     2941 aggatataat gctattacat ttcctaacac gtctttactt cccaaaaaac catataatac
     3001 ggttgctcca attgaaggtt ttttatacaa agcatacaaa ctttgctctt gagaacctgt
     3061 ttgatgcaat agagaaagtt gtaagtaatg agcatttcct tgatttggaa ttgaaatagg
     3121 attttttaca ctaccgtata aataacttaa ttgcacatca ggagtaaaac catttgtagt
     3181 attttcttgt gaataaacac aatacgaata caaaactgat attccaaata acaaaatttt
     3241 cctcacgatt atatacttgt tttttttgct aatatacata ttttatattt tgaagcatat
     3301 caatttatat ggtatttttc acaagttatg agctatttgt ttatttttga aataaattta
     3361 tacggtttta tggttcctat ttcattatct cttcaaggtt tttattcata taaagataaa
     3421 caaaccattg attttaattc tcttaccaaa gcaggtgttt ttggtatttt tggacaaacc
     3481 ggtgcgggca aatctgctat tattgaagct attatgcttg ctctctacgg tgatatagaa
     3541 cgaattaata acagagaaga acgtagttac aatgtaatga atttacaaag taaagaatta
     3601 ttcattgaat ttatttgttt ttcgcatatt actcaaacaa aatacactat aactgttaaa
     3661 gcaaaacgtt cttcaaaaaa ttttgagcaa gtaagtaaac ttgacagaat ctgttacaaa
     3721 cttgttgatt caaagtggat tccgattaat cttgaagata ttcaaaaaga aattacctgc
     3781 ctctcttata ataattttaa acaaactgta attattccac aagggaaatt tatggaattt
     3841 ttacaactat cacctacaga acgtactggt atgctcaaag atttatttaa tcttcatgaa
     3901 tatgaattgt ctgaaaaagt taaaattgtt gaaggcgaaa tcatacaaca aaaatcacgt
     3961 attgaaggaa atatacaaga gattggagaa atagacgaaa cagtattggc tactattcaa
     4021 caaaaagaaa atgaattact tactttaggt attgaacaaa aaaaacaatt agatacgtta
     4081 caagaaaatc taaaaaaatt atatcttatt aaacaagatt ttgatgaatt tgaaaaaaca
     4141 aaactaatac ataaagaatt actatctaaa gaacaagaat tcaaagaatt agaaaaaacc
     4201 atttctctta tttcattatg cgtaaagtat attgctccca tatatgatac gcttcaatta
     4261 aaacaaaacg atgccggtaa attacaatct attattacaa atgctaaaca agaatttcaa
     4321 aaaattgaaa ttcgttttaa cgaaagtaaa aaaatatttg aagaacttga acaaaaattt
     4381 atacaattac ctgaatttaa acaaaataca aatttcataa aacaatgtta tgaattaaaa
     4441 aacgtagaaa agcaaattga aacacaaaca ttgaaacaac aagaatttgc aaaaaaaata
     4501 gaaactttag aaaaaactca aaaaactaat gaatattctc ttcaagaaca aaaagaactt
     4561 cgaaaacaat tacaacaaaa tgtacctgat ataagctcta tttataaaca aaaacatgaa
     4621 ttagaaaaat acaatactat ttgtaaccgt attgaagaat gccgcgaaaa acgcattcaa
     4681 tatgaacatg aaatacaaac acttaaacaa caagtttgtg attctgcctt tactgttttt
     4741 aaacaaaaaa ttgattataa agatataaaa gaaattattg aagataaaat aaaacactgt
     4801 gaaaatataa tttctcacat agatgccgaa ttacagcaag ccctactttt caatcatgta
     4861 aacactttac gaaaaacctt acaacatgga acagcatgcc cggtatgtgg ttctactgaa
     4921 cataatattg aaaaaattgc atcagaatat gaaccaaata ttcaagatat tgaacagaaa
     4981 aaaatacgta tttcatttga attgcaatcc tacacaaaac tattacaact tctacacact
     5041 accaataatc acataactgt attgcaaaaa caagatcacg agatttctca tactatgcat
     5101 tctttaacca ttgaacgcaa tttacaagaa cctgattttg aaatacgtga catatctgtt
     5161 tttgaaaaag aattagaacg aataacttct attcagcaac aaatgaaaaa cattgaaaac
     5221 gagctgattg aatttgagaa aaaacaaaaa gatattattt ctgaactaac tattgctcat
     5281 gaacaattaa tacacataaa aacatcacat accgaaacaa aaacacgttt ttcaatgtta
     5341 aaagaatcta taccttcttc tctttataat acatacggtt catttgatga agattctctt
     5401 atacaagaaa ttgaaaaacg cagcaaacaa cagattgaaa tagaaactag ctatactagc
     5461 ataaaacaag aatacatatt actcaaagaa caatataatt tattaaaaat aagcatttta
     5521 gaaaaagaaa aatcatacaa aatcatttgc gaagatattc aacagctcca agaaaaatta
     5581 acattcgcta ttaatactca ttcatttgac tctacagaac agattgaaaa tttattgaat
     5641 caaaaagaat cattactcga atactcgcaa agattagaac actataaaca tgctctttca
     5701 actattgaaa ctagtgttaa tactctttca aaaaaactag aaggagttaa ttttgatacc
     5761 aatgaatttg aacaattaaa acaaactatt gaaaaagcag aacatgaaat tcaaataaca
     5821 cgagatgctt atattcgtgc aaatgaacaa cgaaaacaat tacaaacaaa attacaaaaa
     5881 caatcagatt tacttaagca attagctata tgtaaaaacg atttagatac aataaccaca
     5941 ttaaagtcat tatttaaaca aagtggattt gtttcatata tgtcaagcaa ttatatacat
     6001 atgatttgca aaaaagcaaa cgaacgattt attctactt
//
LOCUS       DAOTOW010000030         5987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11307_length_5987_cov_3.859238, whole genome shotgun sequence.
ACCESSION   DAOTOW010000030 DAOTOW010000000
VERSION     DAOTOW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11307_length_5987_cov_3.859238"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="P5243_00915"
     CDS             complement(<1..229)
                     /locus_tag="P5243_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18034.1"
                     /translation="MNKILYTVIFCIFSLYTAYAQFVPITNCPQIKINSGNPAFPFPQ
                     FLEYKAGKTLAKYNSEGVTHADMEKTMREAYE"
     gene            complement(395..1432)
                     /gene="cydB"
                     /locus_tag="P5243_00920"
     CDS             complement(395..1432)
                     /gene="cydB"
                     /locus_tag="P5243_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRS18035.1"
                     /translation="METILGLDYPSLWFIIIAVLFIGYAILDGYDFGAGTWHLFFKDN
                     TERRIAINAVGPLWDGNEVWLVIGGGALFAGFPLMYATLFSAMYIPFMLFLLFLIFRA
                     ISIEFRSKEPMAWWTKTWDISYSVSSGMLALLLGVVLGNVIQGMQVGDNFDYAGTGFF
                     EFLTPYPLVIGLLSLSVMMWHGALFLMYKTEGAMQQKIIQLGKIGFIIFVVLYIVATI
                     FTVYAVPQNISVFYSNPVFWIFPLLVPVIIALSYILYSKTKYAYAFICSSLTIGLLLI
                     VAAMGVFPNLLVSTIDPANTITIYNAASSEKTLGIMLLIVSIGAPLLIAYTAIALYIF
                     RGKVKLDETSY"
     gene            complement(1445..2794)
                     /locus_tag="P5243_00925"
     CDS             complement(1445..2794)
                     /locus_tag="P5243_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRS18036.1"
                     /translation="MDVEILARIQFAFTVGFHYIFPPLSIGLGLLMVLFEGMYLKTKN
                     KEYEVLARFWTKIFALTFVLGVSSGIVMEFEFGTNWARYSRFVGDVFGSPLAAEGVFA
                     FALESTFLGILIFGWNKTKPIMHFISTIGVFLGSLFSAVWIVVANSWQQTPAGFEIRG
                     EGAMARAEITDFWAMVFNPSTIDRVLHVWIGALLAGAFLVLSVHAYYLLKGRYIEISK
                     KAFLIALVVATIFSLSQLHSGHASAVGVAQNQPAKLAALEGHYTTGPGDLYILGWVNN
                     ETQEVTGLKVPGGLSFLLHYDFKTPVIGLDQFAKEDQPSQVNAVFQTYHLMVAIGMML
                     IGLTLFACFMWWRKKLFDTRWLLVAFVFAVLLPQIANQVGWIAAEMGRQPWIVYGLLR
                     TSEAFSATVTAPQLLFSLIMFGAIYLLLLVLFIYLLTKKILHGPYDEVTPQSSVSTL"
     gene            complement(2909..4621)
                     /locus_tag="P5243_00930"
     CDS             complement(2909..4621)
                     /locus_tag="P5243_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRS18037.1"
                     /translation="MEFPTSKKELERLGWDYIDVILISGDAYIDHPSFGVAIIAHVLE
                     KVGYRVAILPQPNWRDDLRDFKKLGAPRLFFGISAGSMDSMVNHYTATKRLRSDDAYT
                     PGGLAGQRPDYACVVYANIVRQLYPNTPIILGGIEASLRRFAHYDYWSDRVKQSILLE
                     SKADLLVYGMGEQAITDIAIQLDNGIRVQQIRSVKQTAYIVSSKIELPANCIELHSYE
                     VCSTNKKAYAENFKIIEEQSNSWSPQKIAQQCGNSYVVVNEPYELYTEAELDAIYALP
                     YTRQPHSRYAKKPPIPAFEMIKHSVTMHRGCFGGCSFCTISAHQGKFVVSRSKQSILQ
                     ELSDISRMPYFKGHITDLGGPSANMYRMQGKDLELCKVCKRASCLFPAKCKNLNDDHS
                     HLIALYKESVRIPGIKHVTIGSGIRLDLLQGSKTEREYTSLLLQKHVSGRLKVAPEHT
                     EPQVLSLMRKPPFASFEAFKQVFDEYNTTYGLRQQLIPYFISSHPGCSAHDMKHLSNR
                     LRNLNIMPEQVQDFTPTPMTLATVMYYTGLNPYTGKPVFSAKTKAEKDAQKSSFFWYR
                     KGKM"
     gene            complement(5166..5540)
                     /locus_tag="P5243_00935"
     CDS             complement(5166..5540)
                     /locus_tag="P5243_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18038.1"
                     /translation="MKKFIVFILTCIVCISCDKGSTTKLIITNTTAGNISCFFYTMND
                     VDSLTINKESSKEFKVFTYLVENVPVFKMNYDSIKIIENNNTVKMMYPDSTSSYKSMY
                     NKDNWIFKDNKKGMYTYTYIFN"
     gene            complement(5559..>5987)
                     /locus_tag="P5243_00940"
     CDS             complement(5559..>5987)
                     /locus_tag="P5243_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18039.1"
                     /translation="IVSNNGYGNGLAKDADLCVLGEVWGHHIGWQYTRFKYDSLAEIT
                     KFEHDDFTPVTKGSTISYMRHWIPMGLLLDLQDDTTDIVRINNGVNLIDNANGYTNHM
                     FYNALDSDIKSPQAFKVRLLQENSNLDQTDVNSLFQVYYY"
BASE COUNT         1973 a         1151 c          988 g         1875 t
ORIGIN      
        1 tttcataagc ctcgcgcata gttttttcca tgtcggcatg tgttacaccc tctgaattat
       61 attttgctaa tgttttacct gctttatatt ctaaaaattg cgggaacgga aaagcaggat
      121 tacctgaatt aatctttatt tggggacaat ttgtaatagg aacaaattgt gcatatgcag
      181 tatacaaaga aaatatacaa aaaataactg tatatagtat tttgttcata gtaagtgttt
      241 tttatttagg ataaaattcg tacaaagata atataatata ccaaaatata ggactaattt
      301 tttaggaaat tggttgcatt ttgtacgaaa aaatgttcag tatcaatgaa tttactattg
      361 atactatact tactataaaa aatacaatta agagttagta gcttgtttca tctaatttta
      421 cttttcctct aaaaatatat aatgcaattg ctgtatatgc tataagcaat ggcgctccaa
      481 tagaaacaat aagtaacatg attcccaatg ttttttctga tgaagctgca ttatatatag
      541 taatagtatt tgccggatct attgttgaaa ctaataaatt aggaaatact cccatagctg
      601 ctactatcaa caacaaacca atagtaagcg atgaacagat aaaagcatac gcatacttag
      661 ttttactata caatatataa ctcaaagcta ttattacagg cacaagcaaa ggaaatatcc
      721 aaaatacggg atttgaataa aatacagata tattttgagg tacagcatat actgtaaata
      781 tggtagctac tatatacaat acaacaaata taataaagcc aatttttcct aattgtataa
      841 tcttttgttg cattgctcct tcggttttat acataaggaa caatgctccg tgccacatca
      901 taaccgataa agaaagtaat ccaattacca aaggataggg tgtgagaaat tcaaaaaaac
      961 ctgttccggc ataatcaaaa ttatcaccta cttgcatacc ttgtattaca ttacctaaca
     1021 caaccccaag tagtaaagca agcataccac ttgaaacact ataacttata tcccatgttt
     1081 ttgtccacca tgccataggc tctttactac gaaattctat tgaaatagct ctaaagataa
     1141 gaaacaacaa aaacaacata aatggaatat acatagccga aaacaatgtt gcatacataa
     1201 gcggaaatcc tgcaaacaag gctcctccgc ctattaccaa ccatacctca ttgccatccc
     1261 atagtggtcc tactgcgtta attgcaattc ggcgttcggt attatcctta aaaaataaat
     1321 gccatgtacc tgctccaaaa tcatacccgt ctaatatagc atatcctata aacaatactg
     1381 ctattattat aaaccaaagt gatgggtaat ctaaacctaa aatagtttcc atattcacaa
     1441 gcaattaaag ggttgatact gatgattgtg gtgtaacttc gtcataggga ccgtgaagta
     1501 tttttttggt aagtaagtat ataaacaata ccaataatag taaatatatt gcaccaaaca
     1561 taatcaatga aaataataat tgcggtgctg taactgttgc cgaaaatgct tcacttgttc
     1621 gcaataaacc atatactatc catggttgac gacccatttc ggcagctatc catcccactt
     1681 gatttgctat ttgaggtaat agtactgcaa aaacaaatgc tactaataac caccgcgtat
     1741 caaataactt tttacgccac cacataaaac atgcaaacaa ggttaatcct atgagcatca
     1801 tacctatagc aaccatcaaa tgatacgttt gaaatacggc atttacttgt gaaggttgat
     1861 cttcttttgc aaattgatct aaccctatta ccggtgtttt aaaatcataa tgcaataaaa
     1921 atgataatcc tccgggaact ttaagaccgg taacttcttg tgtttcattg tttacccaac
     1981 ctaatatata caaatcgccg ggaccggttg tataatgccc ttcaagtgct gcaagttttg
     2041 ccggttgatt ttgtgcaacc cccacagccg acgcatgtcc gctatgtaat tgcgaaagag
     2101 agaaaatagt tgcaactacc aatgctatta gaaatgcttt tttagaaatt tcaatatatc
     2161 gtccttttaa taaataataa gcatgcacac tcaaaactaa aaatgcacct gccaataatg
     2221 cacctatcca tacgtgcaac acacggtcaa ttgtagatgg gttaaaaacc atagcccaaa
     2281 aatcggtaat ttcggcacga gccatagctc cctcaccacg tatttcgaaa ccggcaggtg
     2341 tttgttgcca actattcgcc actactatcc acactgccga aaacagagaa cccaaaaaca
     2401 caccaatagt agaaataaag tgcattattg gttttgtttt attccaacca aaaataagaa
     2461 ttcctaaaaa cgtgctttcc agtgcaaatg caaatacgcc ttcggctgca agcggactac
     2521 caaacacatc acccacaaac cgtgagtaac gcgcccaatt ggtgccaaat tcaaattcca
     2581 taactatacc actacttacc ccaagtacaa aggttaacgc aaatattttt gtccaaaacc
     2641 gtgcaagaac ttcatattcc ttgtttttag tttttagata cataccttca aacaacacca
     2701 tgagcaatcc taatcctatg ctcagaggtg gaaaaatgta gtgaaaacct actgtaaagg
     2761 caaactgaat gcgagctaaa atttctacgt ccataacaga atttgttgag tttatacaaa
     2821 tatataatta attttctaat taattgtatt tttttattat tcagatgtat acaaaaaaca
     2881 atattcagac tgaaaaacat gtaaaacatt acatttttcc tttgcgatac caaaaaaacg
     2941 atgatttctg agcatctttt tcagctttgg tttttgccga aaataccggt ttgccggtat
     3001 atggattgag tccggtgtaa tacattaccg tagccagtgt cataggagta ggtgtaaaat
     3061 cttgtacttg ctcgggcatt atgttaagat tccgcaaacg gttcgataag tgtttcatat
     3121 cgtgtgcgct acacccaggg tgcgacgata taaaatatgg tattaattgc tgtcgcaatc
     3181 catatgttgt attgtattcg tcaaatactt gtttaaatgc ttcgaacgat gcaaatggtg
     3241 gtttacgcat aagcgaaagc acctgtggtt ctgtatgttc cggtgctact ttcaaacgac
     3301 ccgatacatg tttttgcaac aaaagcgatg tatattctcg ttcggttttg ctgccttgca
     3361 gtaggtctaa ccgaatacca ctgcctatgg ttacatgctt tatgcccggt atccgaaccg
     3421 attctttata caatgctatc aagtggctgt ggtcgtcgtt gaggtttttg cattttgccg
     3481 gaaacaaaca cgatgctcgt ttgcatacct tacacaattc caaatccttg ccctgcatgc
     3541 gatacatatt tgccgaaggc ccacccaaat cggttatatg tcctttgaaa tatggcatac
     3601 gcgatatgtc cgatagctct tgcagtatcg attgtttgga cctgcttacc acaaatttgc
     3661 cttgatgtgc cgaaatggtg caaaaactac aaccaccaaa acacccgcga tgcatagtta
     3721 ccgaatgttt tatcatttcg aatgcgggaa taggaggctt tttggcatag cgagagtgtg
     3781 gttggcgagt atagggcaat gcatatattg catctaattc ggcttcggta tatagttcgt
     3841 agggttcgtt tactaccaca tagctattgc cacattgttg cgcaattttt tgcggactcc
     3901 aactattgga ttgttcttct ataattttga aattctctgc ataagctttt ttgttggtgc
     3961 tgcaaacctc atatgaatgc aattctatac aatttgcagg taattcaatc ttcgatgaaa
     4021 caatataggc tgtttgtttt acactccgta tttgctgcac tctaatacca ttgtctaatt
     4081 gtatagctat gtcggtaata gcttgctcac ccatgccgta tacaagtaaa tcggccttac
     4141 tttcgagcag tattgattgt ttcaccctgt ctgaccaata gtcgtaatga gcaaatcgtc
     4201 ggagcgatgc ttctatgccg cctaaaataa taggagtgtt ggggtataat tgacgaacta
     4261 tattggcata cactacacaa gcatagtcgg gtcgttgccc tgccaatccg ccgggggtat
     4321 atgcatcgtc ggaacgcagg cgtttggtag cagtatagtg gtttaccata gaatccatgc
     4381 ttccggcact gatgccaaaa aataatcggg gagctcccag ttttttgaaa tctcgcaaat
     4441 cgtcacgcca attgggttgc ggtagtatag caactctgta tccaactttt tctaacacgt
     4501 gagcaataat ggctactcca aacgaaggat ggtcaatata cgcatcacca ctgatgagaa
     4561 ttacatctat atagtcccag cctaatcgtt ccagttcttt ttttgatgta ggaaattcca
     4621 tattacaaga gcgatttggc ggtaaaatta ataaatccga gacataccta ttctaggcga
     4681 gaacctttgt gcatagaata ttttttacaa acatacaatt tatacactaa ttccgcaata
     4741 gttttagagt ggtaggattg gatatgtttc tatttggcac aattaggaaa aaagagagcg
     4801 taaatgccac agtctcattt aggtggagcc gaacaagaaa aggctgctgg gcagtagccc
     4861 aaagttccat acttgtaaca acaataactc tcgaacgtct tacaaaactt gggtatgaat
     4921 ctataacaga atactacaca aaacaccgct ttaaccccga tttagatttt gaatgaaaaa
     4981 aacttttttt ttgaattaca aaatggtaat cgaatgagca attgtttttg cataatgtgg
     5041 ctattcctta ctaaaccgcc gtatacgaaa atccgctgtg gcggatggtg tgagtggctc
     5101 tccgatagac taaaagccta tcggcagtct actcgattac cagcctgtgc tttgtgataa
     5161 ttaatttaat taaaaatata tgtatatgta tacatacctt ttttattgtc tttgaaaatc
     5221 caattatcct tgttatacat agatttataa ctcgatgtac tatctggata catcattttt
     5281 acagtattat tgttttctat aattttaata gaatcataat tcattttaaa taccggaaca
     5341 ttttctacta aatatgtaaa gaccttaaat tctttgcttg attctttatt aatagtcaga
     5401 ctatctacat cattcatagt ataaaaaaag catgaaatat tgccagcagt agtattagta
     5461 attataagtt ttgttgtaga acctttatcg caagaaatac aaacaataca agttaatata
     5521 aatacaataa attttttcat attatatagt ttaatagatt agtaataata cacttgaaat
     5581 aaggaattta catcagtttg atcaagatta gaattttctt gtaataaacg aactttaaat
     5641 gcttgagggg atttgatatc ggagtctaat gcgttataaa acatatgatt agtataacca
     5701 ttggcattat caattaaatt aactccatta tttatacgaa caatatcagt tgtgtcatct
     5761 tgtaagtcta ataataatcc cattgggatc caatgtctca tataactaat agtagagcct
     5821 ttggtaacag gggtaaaatc atcatgctca aatttagtaa tctctgccaa actatcatat
     5881 ttaaaccttg tatattgcca tccaatatga tgcccccaca cttcacctag tacacaaagg
     5941 tcggcatctt ttgcaagtcc attaccatac ccattatttg aaacaat
//
LOCUS       DAOTOW010000031         5792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11751_length_5792_cov_3.910580, whole genome shotgun sequence.
ACCESSION   DAOTOW010000031 DAOTOW010000000
VERSION     DAOTOW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11751_length_5792_cov_3.910580"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="P5243_00945"
     CDS             <1..1124
                     /locus_tag="P5243_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HRS18040.1"
                     /translation="KFTRPVAEIRCGILTIKEKWDLILGCTSSYETQPYLQTKYPRIT
                     NNDTLYINATILPNHALVSEIIQLKKGEALYFQDNIIAVHTTNDFVEQLEEYTQVETL
                     CEVQQIRYPWNIFSFNAQEIERDFELITKHKTSQKISTTNNIIAPENIFIEPDAVVEF
                     ATINASKGKVYIGKGAEVMEGAIIRGPFALCEHATVKMATKIYEGTTIGPYCKVGGEV
                     QNSVLFGYSNKGHDGYLGNSVIGEWCNLGADTNNSNLKNDYSEVKVWNYTTERFMKTG
                     LQFCGLFLGDHSKSAINTMFNTGTLVGVSSNVFGSGFPRNFFPSFSWGGADGMSEYAL
                     KKACETAQRVYARRAKIFDQTEIDIFAHIFQETAQYRRF"
     gene            1202..3637
                     /gene="lon"
                     /locus_tag="P5243_00950"
     CDS             1202..3637
                     /gene="lon"
                     /locus_tag="P5243_00950"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRS18041.1"
                     /translation="MDDSQNSLLHTSVMFNLLGADTEIIPLFTDDDSFDFSDVEVPEA
                     LPILPLKNALLFPGIVMPITVAREKSIELIRDYIKKSKIIGTATQKNSQIENPTIEDL
                     YHIGTVAQILRILEMPDGNITVILQGISRISLIEQLHTEPYLYCAVSKKNDEKPEIDK
                     EFEAILGSIKDMALKIINLSTNIPKESTFAIKNITNPLFLINFISSNVDTAIENKIEL
                     LDIDNVKNRAILLMKYLAQQIQMLEIKNDILTKTRKDIEQQQRDYFLHQQIKQIQNEL
                     GENSIEQEIQEMREKAESKLWPESVKSHFNKELDKLSRMNAHAAEHSVQFNYVQTLLE
                     LPWNEYTEDNFDLQHAQHILDEDHFGLEKVKERIIEHLAVLKLKGDFKSPIICLYGPP
                     GVGKTSLGKSIAKSLNRKYCRISLGGLHDESEIRGHRKTYIGAMPGRIIQNLKKVQSS
                     NPVFVLDEIDKVGKDFRGDPESALLEVLDPEQNNSFYDNYLEQDYDLSKVMFIATANN
                     IANISSALRDRMEMIEVSGYIMEEKIQIALRHLIPHQIVNHGIESTQLSFSKEAIAYI
                     ITHYTRESGVRELDKKIASVVRNYAKKIAMNQSVESDVSVEIVQEILGPELFSPDMYE
                     SNEIAGVVTGLAWTAAGGDILFVETSLSQGKGNLTLTGNLGTVMKESATLALEYIKAH
                     ATQFDIKPEVFETTNVHVHVPEGAIPKDGPSAGITMVTSMLSAFTKRKVKKRLAMTGE
                     ITLRGKVLPVGGIKEKILAAKRAGIHEIIMCSKNKKDVDAVGKQYIHGLKFLYVDTIF
                     DVVQHALLKQKAL"
     gene            3640..4362
                     /locus_tag="P5243_00955"
     CDS             3640..4362
                     /locus_tag="P5243_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18042.1"
                     /translation="MYQTNKLYVTPLLSMTELVTENPSILIMLEHFEADCVLHDDTVS
                     GFCKRHNISLQVFILVANLYNGITPSSIPELSHADIMALIRFLANSHVYYKTQKYPEI
                     ELYISQLSEYNKAPEIPLVTKFFDEYFTEVIEHLEYENSVAFPFFISLLNKQQVSSKV
                     GEFSSQEYKEHHTDIESSLSELKRLLLKHVPIQKDRVLRRKILMSLFELEYDLHKHSL
                     IEEVILTPLMQDIEKNQYNVKK"
     gene            4349..4939
                     /locus_tag="P5243_00960"
     CDS             4349..4939
                     /locus_tag="P5243_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRS18043.1"
                     /translation="MSKSSHSIAIIEPSHIVYEGLVAILLNSGIHYHVSRLESIEELA
                     SFTKEFDILLINPLYIQQNQIQFEQIIVNKEIYTVAIVYALFPNQILKLCNSVIHITD
                     TPEYICSIIEKDCKEHHSYAYEKDKEILSEREIQVLKELVKGYSNKEIADSLCISIHT
                     VISHRKNIVQKTGIKSQAGLTIYALSNNIIALESIQ"
     gene            5043..5115
                     /locus_tag="P5243_00965"
     tRNA            5043..5115
                     /locus_tag="P5243_00965"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5076..5078,aa:Phe,seq:gaa)
     gene            5242..>5792
                     /locus_tag="P5243_00970"
     CDS             5242..>5792
                     /locus_tag="P5243_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRS18044.1"
                     /translation="MDQPKFQRLLRIMKMLTGNTVYTIGDIAQRLEISERTVYRYIDT
                     FREAGFIIKKDGDIFRLDKDSPHFKDINNLLHFSEEEAYILKMAIESIDETHIIKQNL
                     KKKLYTLYNYKIMAESVVKGKIALNVNALTQAIEQKKQVILKGYSSAHSKEISDRLVE
                     PFAFTTNYIQVWAYDTVTKENKL"
BASE COUNT         2149 a          786 c          993 g         1864 t
ORIGIN      
        1 ttaagtttac tcgtccggtt gctgaaattc gatgcggtat tttaactatt aaagaaaaat
       61 gggatttgat acttggttgc acaagttcgt atgaaacaca accatattta caaactaaat
      121 acccacgtat aacaaataat gatacattgt atataaatgc tacaatttta ccaaatcatg
      181 cattagtatc agaaattatc caattaaaaa aaggagaagc attatatttt caagacaata
      241 taatagctgt acatacaacg aatgattttg tagaacaact tgaagaatat acacaagttg
      301 aaactctttg tgaagtacaa caaattcgtt atccatggaa tatattctca tttaatgcac
      361 aggaaattga acgagatttt gaattgatta caaaacataa aacttcgcaa aaaataagta
      421 caaccaataa tataatagca ccagaaaata tttttataga acccgatgct gtagttgagt
      481 ttgctacaat aaatgcttca aaaggtaaag tatatatagg taaaggtgcc gaggttatgg
      541 aaggagctat tatacgtggc ccatttgctt tgtgcgaaca tgctactgtg aaaatggcta
      601 ctaaaatata tgaaggtaca accataggcc cttattgtaa agttggtggt gaagtacaaa
      661 attcagtatt gtttgggtat tcaaataaag ggcatgatgg ttatttgggt aactcggtaa
      721 ttggtgaatg gtgtaattta ggagcagata ccaataattc aaatctcaaa aacgattatt
      781 cagaagtaaa agtgtggaat tatacaacag aacgttttat gaaaaccggg ctacagtttt
      841 gtggattgtt tttaggagat cattccaaat ctgcaataaa tacaatgttc aacaccggta
      901 ctttggttgg agttagttca aatgtgtttg gttccggttt cccacgaaac ttttttccgt
      961 cgttttcatg gggaggtgct gatggtatga gtgaatatgc gcttaaaaaa gcgtgtgaaa
     1021 cagctcaacg tgtatatgct cgtagagcaa aaatctttga tcaaacagaa attgatatct
     1081 ttgcacatat atttcaagaa acagctcaat atcgtcggtt ctaataccat ggcataccta
     1141 ttgacttata tacattacgt aataaattac gtttagtact gacaatttga ctattttaca
     1201 tatggacgat tcacaaaatt ctttattgca cacatcggtg atgtttaatc ttttaggagc
     1261 tgatacagaa ataattccat tatttactga tgatgattct tttgattttt cagatgttga
     1321 agttcctgaa gcactgccaa ttttaccgct aaaaaacgca ttattatttc cgggtattgt
     1381 tatgcctata acagttgctc gtgaaaaatc aattgaattg atacgagatt acattaaaaa
     1441 aagtaaaata attggtacgg caactcaaaa gaattcacaa atagaaaatc ctacaataga
     1501 agacttatat catattggta ctgtagctca aatattgaga attcttgaaa tgcccgatgg
     1561 taatataact gttattttac aaggaatttc aaggatatct ttaattgaac agttgcatac
     1621 agaaccatat ttatattgtg cagtttcaaa aaaaaatgat gaaaaacccg aaatagataa
     1681 agaatttgaa gcaattttgg ggtctattaa agatatggcg ctcaaaatca ttaatttatc
     1741 aacaaatatt cctaaagaat ctacttttgc tattaaaaat atcacgaatc cgttgttttt
     1801 aatcaatttt atatcatcaa atgttgatac cgcaattgaa aataaaattg aattacttga
     1861 tattgacaat gtaaaaaacc gagcaatatt gttgatgaaa tatttggcac aacaaataca
     1921 aatgcttgaa ataaaaaatg atattttaac aaaaactcga aaagatattg aacagcaaca
     1981 acgtgattat ttcctgcatc aacaaataaa acaaatacaa aatgaattag gtgaaaattc
     2041 gattgaacaa gaaattcagg aaatgcgtga aaaggcagaa tcaaaattgt ggccggaatc
     2101 agttaaatcg cattttaata aagaattgga taaattatca agaatgaatg cgcatgcagc
     2161 agaacattcc gttcagttta attatgtgca aacattatta gaactgcctt ggaatgaata
     2221 tactgaagat aattttgatt tgcaacatgc tcaacatatt ttagatgaag atcattttgg
     2281 tttagaaaaa gtaaaagaac gtatcattga acatttggca gtgcttaaac ttaaaggtga
     2341 ttttaaatca ccaattatat gtttgtatgg tcctccggga gttggtaaaa cttcattagg
     2401 aaaatcaatt gcaaaatctt tgaatcgtaa atattgtcga atttcattag gtgggttaca
     2461 tgatgaatcg gaaatacgcg gtcatcgcaa aacatatata ggtgctatgc cgggacgtat
     2521 tattcaaaat cttaaaaaag tacaatcatc gaatcctgtt tttgtattag atgaaataga
     2581 taaagttggt aaagatttta gaggagatcc tgagtcagct ttgcttgaag ttcttgaccc
     2641 ggaacaaaat aatagttttt atgataatta cttagaacaa gattatgatt taagtaaagt
     2701 aatgtttatt gctacagcaa ataatattgc aaacattagt tctgctctgc gtgacagaat
     2761 ggaaatgata gaagtttccg gctatattat ggaagaaaaa attcaaatag cattacggca
     2821 tttaataccg catcaaattg taaatcatgg aatagaatcg actcaattat cattttcaaa
     2881 agaggctata gcatatataa ttactcatta tactcgtgaa tcgggtgtgc gtgaattaga
     2941 taaaaagatt gcaagtgttg ttagaaatta tgcaaaaaaa attgcaatga atcaatctgt
     3001 tgagtccgac gtatctgttg agatagttca agaaatttta ggtcctgaat tgttttcacc
     3061 cgatatgtat gagtcaaatg aaattgccgg agttgtaact ggtttagctt ggactgctgc
     3121 cggtggtgat attttatttg tagaaacaag tttaagccag ggtaaaggta acttaacact
     3181 tacaggtaat ttaggaactg taatgaagga gtctgctact cttgctcttg aatatattaa
     3241 agcacatgct acacaatttg atataaaacc cgaagtgttt gaaacaacaa atgtgcatgt
     3301 gcatgtaccc gaaggtgcta ttcctaaaga tggtccatcg gcgggtatta ctatggtaac
     3361 atcaatgctt agtgcgttta ctaaacgtaa agttaaaaaa cgattagcta tgactggcga
     3421 aataacttta cgagggaaag tgctgcctgt aggtggtatt aaagaaaaaa tattggctgc
     3481 aaaacgtgcc ggtattcatg aaataattat gtgcagtaag aataaaaaag atgtagatgc
     3541 tgtaggcaaa caatatattc atggtctaaa atttttatat gttgatacaa tttttgacgt
     3601 tgtgcaacat gcattattaa aacaaaaagc attataataa tgtatcaaac aaataaacta
     3661 tatgttacac ctcttttgtc aatgacagag ttggttacag aaaatccttc aattttaata
     3721 atgttggagc attttgaagc agattgtgtg ttgcatgatg atacagtttc gggtttttgt
     3781 aaaaggcata atatatcgtt gcaagtattt attcttgttg ctaatcttta taatggaatt
     3841 acaccttcct caattcctga attatcacat gccgatatta tggcattaat tcgtttttta
     3901 gcaaatagtc atgtgtatta caagactcaa aaatatcccg aaatagagtt atatatttcg
     3961 caattatcag agtataataa agctcctgaa atacctttgg ttacaaaatt ttttgatgaa
     4021 tattttactg aagtaattga gcatttagaa tatgaaaatt cggttgcttt tccgtttttt
     4081 atatcgcttt taaacaaaca acaagttagt tcaaaagtag gtgagttttc atctcaagaa
     4141 tataaagaac atcatacgga tattgaatca tctttgagtg aacttaaacg cttattgctt
     4201 aagcatgtac ctatacaaaa agacagagtt ttgcgtcgta aaatattaat gagtttgttt
     4261 gaattagaat atgatttgca taagcattca ttaatagaag aagtaatttt aactccttta
     4321 atgcaggata tagaaaaaaa tcaatataat gtcaaaaagt agtcattcta ttgctataat
     4381 tgaaccatct catattgtat atgaaggttt agtggctata cttttgaatt cgggaataca
     4441 ttatcatgta tctcgattag aaagtattga agaacttgcc tcttttacaa aagaatttga
     4501 tattctactc attaatcctc tatatataca gcaaaatcaa atacaatttg aacaaataat
     4561 tgtaaacaaa gaaatatata ccgttgctat agtatatgca ttattcccaa atcaaatttt
     4621 gaaattgtgt aattctgtaa ttcatataac cgacacgcct gaatatattt gcagtataat
     4681 agaaaaagat tgcaaagaac atcattctta tgcttatgaa aaagataaag aaatattgag
     4741 tgaacgagaa attcaagtac ttaaagaatt agtaaaagga tattcaaata aagaaatagc
     4801 agattcttta tgtataagta tacatacagt aataagtcat agaaagaata tagtacaaaa
     4861 aactggtatt aaaagtcagg caggtcttac tatttacgct ctttcaaaca atattattgc
     4921 cttagaatca atacagtagt aaatagtcgc aattaaatca cagtaaaatt ataaaaaaaa
     4981 cgtctatttt tttttggagt ttgcaaaaaa cgtctatttt tgcactgcat ttttaaaagc
     5041 atggaaatgt agctcagtcg gtagagcaca ggactgaaaa tcctggtgtc ggcggttcga
     5101 tcccgcccgt ttccacagaa aaggttgtca tttttttgtg acaacctttt tgtttttatg
     5161 tattttttat gcttctttct tcaataattt aattgtcaat atgtgtcatt gttgtatatt
     5221 tatgcaataa aatatacaat tatggatcaa ccaaaatttc aaagactttt gcgaataatg
     5281 aaaatgctta caggaaatac cgtgtatact attggtgata tagcacaacg tctcgaaatc
     5341 agtgaaagaa ctgtatatag atatatagat acgtttagag aagctgggtt tataataaaa
     5401 aaagacggtg atatatttag attagataaa gattctccac attttaaaga tattaataat
     5461 ttattgcact tttcagaaga agaggcatat attcttaaaa tggcaataga aagcattgat
     5521 gaaacgcata tcattaaaca aaatttaaaa aagaaattgt atacgttgta taattataaa
     5581 ataatggcag agtctgtcgt taaagggaaa atagctttaa atgtaaatgc tttaactcag
     5641 gcaatagaac aaaaaaagca agttatacta aaaggatata gttctgcaca tagtaaagaa
     5701 ataagtgatc gattggtaga gccatttgca tttactacaa attatattca agtatgggca
     5761 tacgatacag taacaaaaga aaataagttg tt
//
LOCUS       DAOTOW010000032         5778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11785_length_5778_cov_3.581513, whole genome shotgun sequence.
ACCESSION   DAOTOW010000032 DAOTOW010000000
VERSION     DAOTOW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11785_length_5778_cov_3.581513"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="P5243_00975"
     CDS             <1..360
                     /locus_tag="P5243_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18045.1"
                     /translation="QDGFVFQGPMGAYGGSILLLWALQDEEDYYAMGILGFFIPEGLT
                     FHIPFGENTILSPYFNILALQHSVYRVSPYFEGGFKIKQYFTDRAFLQLDFGISSAYT
                     TATTRNFIGAGIGLDML"
     gene            complement(362..1591)
                     /locus_tag="P5243_00980"
     CDS             complement(362..1591)
                     /locus_tag="P5243_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS18046.1"
                     /translation="MLSHSNTTSQIHSQFEIFNNLPVAVWVENASMLYRELQKISVSY
                     NGTIQQFLERNPHELPRLIDLIQIIDVNDFAVSLYKASHKNQLIKGIRNFFIKESFHD
                     FTKMLVSLYAGSTEYSYTSSKITVSGHKVITQVTVKIPQHALHDWSQMIVTETDISQF
                     IKREVALKIQSDKIKQDNTNKEKILSIIAHDLKNPFNTILGFSELLLTKFNTFTQEQV
                     SDFLKYIHESANQSYHLLSNLLNWSKMQQSGVSKNLQIFHLEPLISNISQLIHTTCVS
                     KNITIHQRIAKDIEVYADINMLAFVIRNVLTNAIKFSYKNSDIFIHAYEQNTDVVIEI
                     ADEGIGMDIDTQLQLFDPEKISSRRGTENECGTGIGLMLSKEFIEKQHGSLEIESEMG
                     KGTKVTLRIPKEKKQYL"
     gene            complement(1670..3019)
                     /gene="argH"
                     /locus_tag="P5243_00985"
     CDS             complement(1670..3019)
                     /gene="argH"
                     /locus_tag="P5243_00985"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRS18047.1"
                     /translation="MAQHQSPIWAKDTTAHEKVSLFTVGRDKEFDTLLAEADILGNLA
                     HTRMLQSIGLLTQKELDLVQSELIAMFRDIQISPFVIEEGVEDVHSQVELMLTRKLGD
                     VGKKIHSGRSRNDQVLLDLKLYIRQQIFDTVEYVEQLFVLLQQLSEQHKDVLMPGYTH
                     LQIAMPSSFGLWFGCYAESLTDDLQLLQAAFTITNQNPLGSAAGYGSSFPLNRTMTTE
                     LLGFKTLNYNVMYAQMGRGKVEKTVSYALAGIASTLSKMAMEMCLFMNQNFGFVSFPE
                     EFTTGSSIMPHKKNPDVFELIRAKCNRMQVLPIEISSIISNLPFGYNRDLQIIKEHFL
                     PAFSELHSCIDMMILMLQNITVSKTILDSPMYDYLFSVDEVNNLVQQGVPFRDAYIKV
                     GLDIKQGNFNAPKQAKHTHEGSIGNLCTEQIRMKFQEIKQQFNKDYVTKKIQDLTSS"
     gene            complement(3025..4080)
                     /locus_tag="P5243_00990"
     CDS             complement(3025..4080)
                     /locus_tag="P5243_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRS18048.1"
                     /translation="MHTELYTFAKQTLIELIRIPSYSKSEDKTADLLQRIIESYSVPV
                     SRIGNNIIARSAIPSHKPCVLLNSHHDTVKPVAGWTTNPHEAIIDNGKIIGLGSNDAG
                     APLVCLLATFIYFCKNPHNVYDFLFVASAEEEISGLQGVELVLPHIEPIEFGIVGEPT
                     SMRMAIAEKGLLVLDCKSVGIAGHAAHSTGENAIYKAITDIEWFKTFVFPKQSELLGP
                     VKMTVTGIQAGIQHNIIPAECTFMVDIRTNELYTNQEVYEIVKQHVHCEVTPRSFRLQ
                     SSYISPQHMLVESAKQLNIELFGSPTMSDQACMRNFPTVKIGPGDTMRSHTADEFIYE
                     SEIEQGIDVYIQLLLSL"
     gene            complement(4117..>5778)
                     /locus_tag="P5243_00995"
     CDS             complement(4117..>5778)
                     /locus_tag="P5243_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18049.1"
                     /translation="RWSHQDIELYLFENKIQQLSIYNAKRISQFSYGWKFSKQVSSND
                     FSVTIPPFSKNAFTTEILDDSVIVWFHDANLIQKDEAAIIVTYGTKTDTLHLFAMKEF
                     PNRLKCELNPPKSVVFPGDTIQVRFQRPIQNYENRMYMARVKDTTMYSNMWNKPHVLN
                     NDKVPEITKQIAPLQQQYSSKFFYQKQDLLSYKIGLNRCALYFAQPISVNEITLQLEA
                     FPNITNWYELEQDVSSNAILCWITHPDIMKLKNICFTVEFMSDNKKQIKTIEFNPGFS
                     KKDSFKRAQVGNLIPDLLESQKKELLLDNAIQVVCNNPIQEFSVNLFSLVAQSDTTQT
                     SCISKIELYGKRSLLIYYSAKPGTKYILTLQKNAITDVYGSHNKQKIFTLQTQTKNAT
                     KLFVPIVAHIKNKDSRNFLIQAPRESGNYAIIFPKASCTDIYGDVNDSIAFMVTVPKP
                     ENFGTLQLSVTSQSQNIVIQLYKKDSKSKKPDYMQSQSKSGVFSFVNVQPGEYTAYAI
                     FDNNCNQKWDTGSLEQKEQPEKIEILETPIIIKANWDNVVQWKID"
BASE COUNT         1884 a          963 c          820 g         2111 t
ORIGIN      
        1 caagacggat ttgtgtttca aggaccaatg ggggcatacg gaggttctat tttactttta
       61 tgggcgctac aagacgaaga agattactat gctatgggaa tacttggttt tttcataccc
      121 gaaggtctta catttcatat tccatttgga gaaaacacaa tactatcacc atactttaat
      181 attttagctt tacaacattc tgtatataga gtatcaccat attttgaagg tggtttcaaa
      241 ataaaacaat atttcacaga tagagcattt ttgcaattag attttggaat ttcatcagct
      301 tatactacag ctaccacaag aaattttatt ggagcaggaa ttggtttaga tatgctgtaa
      361 tttataaata ttgtttcttt tctttcggta ttcgcaatgt aacttttgtt cctttaccca
      421 tttcactttc tatttcaagt gagccatgtt gtttttcaat aaattcttta ctcaacataa
      481 gtccaatacc tgttccacac tcattttcag ttcctcgacg acttgaaatt ttctccgggt
      541 caaacaattg caactgcgta tcaatatcca tacctatacc ttcgtctgca atttctatta
      601 ccacatcagt attctgctca taggcatgaa taaaaatatc tgaattttta tatgaaaatt
      661 taatagcatt tgtaagtaca tttctaatta caaatgcaag catattaata tcagcatata
      721 cttctatatc tttggctata cgttgatgta tcgtaatatt ttttgaaaca caagtagtgt
      781 gaattaattg actaatatta gaaatcaaag gttctaaatg aaaaatttgc aaatttttag
      841 acacacccga ttgctgcatt ttactccaat taagcaaatt tgacaataaa tgataacttt
      901 gatttgcaga ctcatgaata tacttcaaaa aatcagaaac ttgctcttga gtaaacgtgt
      961 taaactttgt taataataat tccgaaaatc ctaaaatggt attaaaggga ttttttagat
     1021 catgagcaat tattgaaagt attttttctt tattagtatt atcttgtttt attttatcgg
     1081 attgaatttt aagtgctact tcacgcttga taaattgaga aatatctgtt tcggtaacaa
     1141 tcatttgtga ccaatcatgc aaagcatgtt gaggaatttt tacagtaact tgtgttataa
     1201 ctttatgtcc tgaaactgta atttttgacg atgtataact atattcagta gaacctgcat
     1261 ataaactcac caacattttt gtgaaatcat gaaaactttc ttttataaaa aagtttctaa
     1321 ttccttttat caactgattt ttgtgagaag ctttatataa acttacagca aaatcattaa
     1381 catcaattat ttggattaaa tcaattaatc gaggtaattc atgaggattt ctttctaaaa
     1441 actgctgaat agtaccattg tatgatacag atattttttg caactcacga tacaacattg
     1501 aagcattttc tacccatacc gcaaccggca gattattgaa aatttcaaat tgcgaatgta
     1561 tttgacttgt tgtatttgag tgcgataaca tacattgtaa aaattacact aaactactac
     1621 tttactacaa ataacttacg tttaaatcat aaatcttttt aacaaattac taactacttg
     1681 ttaaatcctg tatttttttt gtaacgtagt ctttattaaa ttgttgttta atttcttgaa
     1741 atttcatacg tatttgttct gtacaaagat ttccaatact tccttcatga gtatgtttag
     1801 cttgttttgg agcattaaaa tttccttgtt taatatctaa accaacttta atataagcat
     1861 cgcgaaatgg cacaccttgt tgtactaaat tattaacttc atctacacta aacaaataat
     1921 catacatagg tgaatcaaga atagtttttg ataccgtaat attctgcaac attaatatca
     1981 tcatatcaat acacgaatgc aattcgctaa aagcgggtaa aaaatgttct tttataattt
     2041 gtaaatctct attatatcca aaaggtaaat ttgaaataat actactaatt tcaataggca
     2101 atacttgcat tctattacat tttgcacgaa ttaactcaaa tacatcagga ttctttttat
     2161 gaggcattat acttgaccct gtagtaaatt cttcggggaa tgatacaaat ccaaaatttt
     2221 gattcataaa caaacacatt tccattgcca ttttagagag agttgacgca attcctgcta
     2281 atgcataact tacagttttt tcaactttac ctcgtcccat ttgagcatac attacattat
     2341 aattcaatgt tttgaatcct aacaactcgg tggtcatagt tctattaagt ggaaacgatg
     2401 aaccatatcc tgctgccgag ccaagtggat tttggttagt aatagtaaat gctgcctgaa
     2461 gcaattgcaa atcatcggtt aaactttcgg cataacaacc aaaccataac ccaaacgatg
     2521 atggcattgc aatttgcaaa tgagtatagc ccggcatcaa cacgtcttta tgttgctcac
     2581 ttaattgttg taacaataca aataattgtt ctacatactc tactgtatca aaaatttgtt
     2641 gacgtatata caattttaaa tctaataaaa cctgatcatt tcgcgaacgt ccgctatgta
     2701 tttttttccc tacatctccc aatttacgag taagcatgag ttctacttga gaatgaacat
     2761 cttcaactcc ttcttctatt acaaaaggtg atatttgtat atcacgaaac attgcaataa
     2821 gttcactttg cactaaatct aattctttct gtgtcaacaa acctatactt tgcaacatac
     2881 gtgtatgagc caaatttcct aaaatatcgg cttctgctaa caatgtatca aattctttgt
     2941 cgcgaccaac agtaaataaa cttacttttt cgtgtgctgt agtgtctttt gcccatattg
     3001 gcgattgatg ttgagccata attattataa agataaaagt aattgaatat atacgtctat
     3061 accttgttct atttctgatt catatataaa ttcatcggca gtatgagatc tcatagtatc
     3121 acccggtcct atttttacgg ttggaaaatt gcgcatacat gcttgatctg acatagttgg
     3181 tgaaccaaac aactctatat ttaactgttt agccgattct actaacatgt gttgaggcga
     3241 tatatatgat gattgtaatc taaaagaacg tggagtcacc tcacaatgaa catgttgttt
     3301 tacaatctca tacacctctt gatttgtata taattcattt gttcttatat ctaccataaa
     3361 tgtacattct gcaggtatta tattgtgctg tattcctgct tgtatacctg ttactgtcat
     3421 ttttacaggt cctaacagtt ctgattgttt aggaaaaaca aatgttttga accattcaat
     3481 atctgtaatt gctttataaa tagcattttc accggtactg tgagccgcat gtcctgctat
     3541 acctaccgac ttacaatcaa gcaccaataa acctttttcg gctattgcca ttcgcatact
     3601 tgtaggttct cctacaattc caaactctat tggttctata tgcggcaata ccaattctac
     3661 tccttgcaag cctgaaattt cttcttcggc agacgcaaca aataaaaaat catatacatt
     3721 atgaggattc ttacagaaat aaataaaagt tgcaagtaaa cataccaatg gagcaccggc
     3781 atcattacta cctaatccta ttatttttcc attgtctata attgcttcat gaggattggt
     3841 tgtccatcct gcaacaggtt ttaccgtatc atggtgagaa ttgagtaaca cgcatggttt
     3901 atgtgaaggt attgctgaac gtgcaattat attattacca attcgtgata caggtactga
     3961 atatgattca ataatgcgtt gtaacaaatc agccgtttta tcttctgatt tactgtatga
     4021 cggtatgcga ataagctcta ttaaagtttg ttttgcaaaa gtatataatt cagtatgcat
     4081 gccgttacaa ttttacatat acaaatatag agttttttag tcaattttcc attgaaccac
     4141 gttgtcccaa tttgctttaa ttataatagg agtttctaaa atttcaattt tttcgggttg
     4201 ttctttttgt tccaaacttc cggtatccca tttttgatta caattattat caaatattgc
     4261 atacgctgta tattctcctg gttgtacatt aacaaaagaa aacactcccg attttgattg
     4321 agattgcata taatcaggtt tttttgactt agaatctttt ttatataatt gaattacaat
     4381 attttgactt tgagaagtaa cagaaagttg aagtgtacca aaattttcag gttttggtac
     4441 agtaaccata aaagcaatag aatcatttac atcaccatat atatctgtac atgatgcttt
     4501 aggaaaaata attgcgtaat tacccgactc acgtggtgct tgaattaaaa aatttctcga
     4561 atctttattc ttaatatgtg ctacaatagg aacaaataat tttgttgcat ttttagtttg
     4621 agtttgcaat gtaaatattt tttgtttatt atgagaacca tacacatcgg tgatagcatt
     4681 tttttgaagt gtaagtatat attttgttcc cggttttgca gaataatata ttaataaaga
     4741 acgctttcca tacaattcaa ttttagaaat acatgatgtt tgagtagtat ctgactgtgc
     4801 tactaatgaa aataagttta cagaaaattc ttgtatagga ttattacaaa caacttgaat
     4861 agcattatca agtaataatt cttttttttg actttcaagt aaatcgggaa tcagattacc
     4921 aacctgagct cgtttaaacg aatctttttt tgaaaacccg ggattaaact caatcgtttt
     4981 tatttgtttt ttattgtcgg acataaattc aacagtaaaa caaatatttt ttaatttcat
     5041 tatatcggga tgcgtaatcc aacataaaat agcatttgaa gaaacatctt gctctaattc
     5101 ataccaattt gttatatttg gaaatgcttc caattgtaac gtaatttcat ttacactaat
     5161 tggttgagca aaatacaacg cacatctgtt taacccaatt ttatacgaaa gcaaatcttg
     5221 tttttgatag aaaaactttg aagaatattg ctgttgtaac ggtgcaattt gttttgtaat
     5281 ctctggtact ttatcgttat ttaacacatg aggcttattc cacatattgc tatacatagt
     5341 agtgtctttt acgcgagcca tatacattct attttcgtag ttttgaatcg gtcgctgaaa
     5401 ccttacttga atagtatcac ctgggaaaac tactgattta ggaggattta attcacattt
     5461 taatctattt gggaactctt tcattgcaaa taaatgtaat gtatctgttt ttgtaccata
     5521 cgttacaatt atagcagcct catctttttg aatcaaatta gcatcatgaa accaaactat
     5581 tacactatca tctaatattt cggtagtaaa tgcattttta gaaaaaggag gtatagtaac
     5641 agaaaaatca tttgagctaa cttgtttaga aaatttccaa ccatatgaaa attgagaaat
     5701 acgttttgca ttataaatac tcaactgttg aattttattt tcaaataaat acaattcaat
     5761 atcctgatga gaccaacg
//
LOCUS       DAOTOW010000033         5746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11856_length_5746_cov_4.063785, whole genome shotgun sequence.
ACCESSION   DAOTOW010000033 DAOTOW010000000
VERSION     DAOTOW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11856_length_5746_cov_4.063785"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="P5243_01000"
     CDS             <1..796
                     /locus_tag="P5243_01000"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HRS18050.1"
                     /translation="INRLSIGIQSFFDEDLQKLNRRHTSAEAQNCVKLAQEVGFTNIT
                     VDLIYGLPNQSLERWQQNLDKVSQLNVQHLSCYALTVEKKTALAQLVKRKQVIPQSDD
                     AYVEQYNYLIQWCKQHGFEQYEISNFAKQGMFSRHNSNYWSGKPYLGVGPSAHSFNGT
                     HRFWNSAHNQKYIDALQNNKLLQESEQLSHTDMCNEYIMTALRTVQGIEIQRMQQLMT
                     SEEFADFMKQVQTLIRSQKLCMSQSHIYFTSQGFMVSDMIIAELFV"
     gene            complement(962..1360)
                     /locus_tag="P5243_01005"
     CDS             complement(962..1360)
                     /locus_tag="P5243_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRS18051.1"
                     /translation="MKLSQNYSEKNTEMLAKQIVNAAFKVHKNLGPGLLEKVYEVCFC
                     YELSKVGIQTKRQVEIPISYEQMIFNEALRLDVLVEDSIICELKAVDILNPVWEAQII
                     SHLKLSNKRIGFLINFNVPVIKQGIKRYIV"
     gene            1601..3073
                     /locus_tag="P5243_01010"
     CDS             1601..3073
                     /locus_tag="P5243_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS18052.1"
                     /translation="MSILKKLAGQTAVYGLSTIVGRFLNFLLVPLYTRFFDQSEYGVV
                     TELYSYTVFLMIFLTYGLETGFFRFSQNNDDKPNVYTTIMSSLFTSSSIFIALVFIFL
                     EPLSRALGYVGFEKYIWWMALIIGIDAFTSVPFAKLRHEQKAIQFAVIKITNIAINIG
                     LNILLIVVLPRIAQTHDIAWIDQHFAQPSVASIFISNLVASIVTLIMLLPQILRNHNS
                     FDFSLLKRILAYSWPLLIAGLAGSINEVYDRISLRYFLTIPHGVDASQYILSQVGVYG
                     ANYKLAMIISIFNQAFRFAAEPFFFSRMHATDSRETYAKIMNYFVAFTLFLFLIIMLY
                     IDVFKHFIGSNFHEGLSVVPILLLANIFLGIVFNLSIWYKLSNQTKFGIWIVGFGALI
                     TLIINMLFVPHYGYVACAWATLACYVLMTILSYRLCQKYYVIPYNLKRLGLYFGLAAL
                     FYCVDAFFPYTHTVVMYACKTVMIAIFVGIVYVNERRQLI"
     gene            3213..4343
                     /locus_tag="P5243_01015"
     CDS             3213..4343
                     /locus_tag="P5243_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18053.1"
                     /translation="MNQIQRTLQQEIEKRLKPNKVMLLLGARRVGKTILIKKIISNFT
                     GKVLMLNGEDSDSLVLLANRSISNYQNLLKGIDLLVIDEAQNVPEIGQKLKLIVDEIP
                     NIKVIASGSSSFDLLNFTGEPLVGRSYTLYLHPFSQQELGAIENALETKQNLESRLIY
                     GSYPELISLDNTDKKEYLNNIVNSYLLKDILIIDGIRNASKMLDLLRLIAFQTGNLVA
                     YDELGKQLGMSKNTVEKYLDLLSKVFVIYRLGAYSKNLRKEISKSSKWYFYDTGIRNA
                     IIGNFAPLALRNDVGQLWESYILSERIKQKCFNVLNTQHFFWRTYDGQELDIVEIEDT
                     TIRAFECKWNTAKAKIPVAFAQAYSEAQFSVISQDNYLEFIG"
     gene            4355..5068
                     /locus_tag="P5243_01020"
     CDS             4355..5068
                     /locus_tag="P5243_01020"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HRS18054.1"
                     /translation="METIYHFTKISTLVELILPTMLLKLNSIENMNDPRETKIFGFSQ
                     ITDSVDNSSLEKNNKEFSDFIKKNIKICSFVKNATVGDSIIEGFKNTMMWAHYGDNHK
                     GVSIALNYNKIKEKNKKIKFEKIEYFKPNNNFLQIDKQEYNKDKKKYRENLFINEETL
                     KKLFFQKTWEWSNENEIRALIKNGKKKDDFIKIKDCIEMIYLGVDCSENYIPSIQKYI
                     SPEKIKKLKFEYDRIRIKQ"
     gene            5217..5636
                     /locus_tag="P5243_01025"
     CDS             5217..5636
                     /locus_tag="P5243_01025"
                     /inference="COORDINATES: protein motif:HMM:NF021049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HRS18055.1"
                     /translation="MDNLSIPFHSFLNIQKSDNPDFILMIGVRPEYLNHVGTVHACVQ
                     VTLAEATSGEFLDTQIPGLLSGAFMPVVRKSQHKYHKPANGALFAKARVIESNITQIS
                     QEIHEKGRSLFQIEIKVFDSHNQNTLTSVFDWYIGKI"
BASE COUNT         1994 a          856 c          954 g         1942 t
ORIGIN      
        1 aattaatcgc ttaagtattg gtattcagtc atttttcgac gaagatttac aaaaattaaa
       61 cagacgtcat acttctgccg aagctcaaaa ttgtgtaaaa cttgcacaag aagttggttt
      121 tacaaatatt acagttgatt taatatatgg cttgccaaat caatcattgg aacgctggca
      181 acaaaatctt gataaagtat cgcaactcaa tgtgcaacat ctttcgtgtt atgccttaac
      241 ggtagaaaag aaaacggctt tggcacaatt agtcaaacga aaacaagtaa ttccgcagag
      301 cgatgatgca tatgtagaac aatataatta tctgatacag tggtgtaaac aacatggatt
      361 tgaacaatac gaaatttcaa attttgcaaa gcaaggtatg ttctctcggc ataattctaa
      421 ttattggagc ggaaaaccat atttaggtgt tggcccttcg gcacattcgt ttaacggtac
      481 gcatagattt tggaatagtg ctcacaatca gaaatatatt gatgcattgc agaacaataa
      541 acttttgcaa gaatcagaac aattgtcgca taccgatatg tgtaatgagt acataatgac
      601 tgcattgcga acagtacaag gtattgaaat ccaaagaatg cagcaattga tgacgagtga
      661 agaatttgcc gattttatga aacaagttca aacgttgata cgatcacaaa aactttgtat
      721 gtcgcaaagt catatttatt tcacttcgca gggttttatg gtgtccgata tgattatagc
      781 ggagttgttt gtataggtta ttttagccac taagtcacta agacacaaaa aatataataa
      841 taataaatct tggtgccttc gtgcctttgt ggcaaagaag aaataatagc catcaagtca
      901 ctaagacaca aagaaaagta aaataataaa tcttagagtc ttcgtgtttt tgtggcacta
      961 atcaaactat atatctttta ataccttgtt ttatgactgg tacattgaag tttattaaaa
     1021 aaccgattct tttatttgaa agttttaagt gactaataat ctgtgcttcc caaactggat
     1081 ttaatatatc cacagccttt aattcacata ttatggaatc ttctaccaat acatctaaac
     1141 gtaaagcttc attaaatatc atttgttcgt atgaaatggg aatttctact tgacgttttg
     1201 tttgaatacc tacttttgat aattcataac aaaaacaaac ttcataaact ttttccaata
     1261 atccgggtcc aagatttttg tgtactttaa atgctgcgtt tacaatttgt tttgcaagca
     1321 tttctgtatt cttttcagaa tagttttgcg ataatttcat taaaaatgtt agttttagtg
     1381 ttggtgatgc aaatataata aaaattaata gccactaagt cactaagaca caaagaaaaa
     1441 tattataaaa aatcttagag tcttcgtgcc tttgtggcaa ataagaaata atagccacca
     1501 agtcacaaag acacaaagaa aagtaaaata ataaatctta gagccttcgt gccttcgtgg
     1561 caaaaaaata taattatctt tgttatttat taattaaaaa atgagcattt taaaaaaact
     1621 cgcagggcaa acagcagtat atggtttaag tactattgta ggaaggtttc taaatttttt
     1681 gttagttcct ctctatacac ggttttttga ccaatcggaa tatggtgtag taaccgagtt
     1741 gtatagctat accgtgtttc ttatgatttt tcttacctac ggattagaaa ccggtttttt
     1801 ccgtttttcg caaaacaacg acgataagcc taatgtgtat actaccatta tgagcagctt
     1861 gtttacctca tcgagtatat ttatagcatt ggttttcatt tttttagaac ctctttcgag
     1921 agcactaggc tatgtcggtt ttgagaaata tatttggtgg atggcactga ttatcggtat
     1981 cgatgcattt acctcggtac cctttgcaaa gttgcgtcac gaacaaaaag ccatacaatt
     2041 tgccgtaatt aaaataacaa atattgccat aaatattggc ttaaatattt tgttgattgt
     2101 ggtcttgccg cgcatagctc aaactcacga tatagcttgg attgaccagc attttgcaca
     2161 accatcggta gcatctattt ttatttcaaa tttggtagca agtattgtta cacttatcat
     2221 gcttttacct caaattttac gaaatcacaa ttcgttcgat ttttcattac tcaaacgtat
     2281 acttgcatat tcgtggccat tgcttatagc aggcttagcc ggaagtataa acgaagtgta
     2341 tgacagaatt tcgctccgtt attttcttac tattccccat ggcgtagatg cttcgcaata
     2401 tatactgtcg caagttggtg tgtatggagc caattataaa ttggcaatga ttatttctat
     2461 atttaatcaa gcctttcggt ttgctgccga accattcttt ttctcgcgaa tgcatgctac
     2521 cgattcacgc gaaacgtatg ctaaaattat gaattatttt gttgcattta ccttgttttt
     2581 gtttttgata ataatgctgt atatcgatgt atttaagcat tttattggga gtaattttca
     2641 cgaaggattg tcggtagttc caatactttt attggctaat attttcttag gtattgtatt
     2701 taacctttct atttggtaca aactgtcgaa ccaaacaaaa ttcggtatat ggatagttgg
     2761 ttttggagca cttattacat taattataaa tatgctattt gttcctcact atggttacgt
     2821 agcctgtgca tgggcaacac ttgcatgtta tgtgttaatg acaattctct cctatcgttt
     2881 atgtcaaaaa tattatgtaa ttccctataa tttaaaacgt ttaggtttat actttgggct
     2941 agcagcactg ttttattgtg ttgatgcgtt tttcccatat acccataccg tggtaatgta
     3001 tgcctgcaaa acagttatga ttgctatatt tgttggtatt gtgtatgtaa atgaacgaag
     3061 acagttgata tagtttgcaa tcgggaaatg gtaaaatcgt cagtatattg acaaaattta
     3121 tataaaaatt gtcaacgtaa tgacaaaatt tatataaaaa ttgtcaatac atattttttt
     3181 atcatatatt tgtgctatta aatagtatga atatgaacca aatacaacgt actttacaac
     3241 aagaaataga aaaacgctta aagccaaata aagttatgtt gcttttgggg gctcggcgtg
     3301 ttggtaaaac catattaatt aaaaaaatca taagcaattt taccggtaaa gttcttatgc
     3361 ttaatggcga agattccgat agtttggtat tattggcaaa caggagtatt tcaaactatc
     3421 aaaatcttct aaaaggaatt gatttacttg taattgacga agctcagaat gttcccgaaa
     3481 tcggtcaaaa gttgaaatta atagtcgatg aaattccaaa tatcaaagtc atcgcaagtg
     3541 gttcatcatc gtttgattta ttaaatttta cgggcgaacc attggttgga cgaagttata
     3601 cattgtattt gcatcctttt agtcagcaag aacttggagc aattgaaaat gctttagaaa
     3661 caaaacaaaa tttagaatct cgtctcatat atggttctta ccccgagctt atttcactcg
     3721 acaatactga caaaaaagag tatttgaata atattgtaaa ttcatatttg ttaaaagata
     3781 ttttaataat tgatggtatt cgtaatgcat caaaaatgct cgatttgttg cggcttatag
     3841 catttcagac aggtaatttg gttgcctacg acgagcttgg aaaacaattg ggaatgagca
     3901 agaatactgt agaaaaatat ctcgatttgc tttcgaaagt gtttgtaata tatagattag
     3961 gtgcgtattc aaagaattta cgcaaagaaa tttctaagtc gtcgaaatgg tatttttatg
     4021 ataccggaat ccgcaatgcg ataattggaa attttgctcc attggctctc cgaaatgatg
     4081 ttggtcaatt atgggaatcg tatatacttt ctgaacgaat taagcaaaaa tgttttaatg
     4141 tactgaacac acaacatttt ttttggcgca cctacgacgg acaagagctt gatatagtag
     4201 aaatagaaga caccacaata cgtgcatttg agtgtaaatg gaatactgca aaagccaaaa
     4261 ttcccgtagc atttgcacaa gcatactccg aagctcaatt ttcagttatt tcacaagaca
     4321 attatttgga gtttataggt taataattat caatatggaa acaatatatc atttcacaaa
     4381 aatatcaact ttggtagaat taattttacc aacaatgtta ttaaaattga attcaataga
     4441 gaatatgaat gatccaagag aaactaaaat ttttggattt agtcaaataa ctgattctgt
     4501 tgataattca agtttagaaa agaataataa agagttttca gattttatta agaaaaatat
     4561 taagatttgt agttttgtta aaaatgcaac tgttggagac tcgattatcg agggttttaa
     4621 aaatacaatg atgtgggcac attatggtga taatcataaa ggagtaagta tagcgttgaa
     4681 ttataataaa ataaaagaaa aaaataaaaa aataaaattc gagaaaatag aatattttaa
     4741 accaaataat aattttttac aaattgataa acaagaatat aataaagata aaaaaaaata
     4801 cagagaaaat ttatttataa atgaggaaac gctaaaaaaa ttgttttttc aaaaaacatg
     4861 ggaatggagt aatgaaaatg aaattcgtgc tttaattaaa aatggaaaga aaaaagatga
     4921 ctttattaaa attaaagatt gtattgaaat gatttattta ggtgtagact gttcagaaaa
     4981 ttatatacct tctattcaga aatatatttc accagagaaa attaaaaaat taaaatttga
     5041 atatgatagg attcgaatta aacaataatt tttttatatg taacctttca aaaattaagt
     5101 tgtacatttg agcaaactac aaatagtagt attagagttt actataaata tgcttccatt
     5161 gcaagaatct gatttctagt accttctaca ggttacatac tagaaatttt taattcatgg
     5221 acaatctttc aattcctttt cattcgtttt tgaacattca aaaatccgat aatcccgatt
     5281 ttatattaat gataggtgtt cgccccgaat acttaaatca tgttggtact gtgcatgcgt
     5341 gtgtacaagt tacccttgcc gaagctacca gtggcgagtt tttagacacc caaataccgg
     5401 gtttgctcag tggagccttt atgcctgtag tacgtaagtc gcagcataaa tatcataaac
     5461 cagccaatgg agctttgttt gcaaaagcac gagttattga atcaaacatt actcaaataa
     5521 gtcaagaaat tcacgaaaaa ggacgctcgc tctttcaaat agaaattaaa gtatttgatt
     5581 cgcacaatca aaataccctt acttctgtgt tcgactggta tataggaaaa atataacaaa
     5641 ccgtttaaat ctctcgtttc atttttttat aacttgttta caatcttgca tgcctaattg
     5701 caaatcaaaa taaaaaaaca ttgtattttc acgttttatt tgaaat
//
LOCUS       DAOTOW010000034         5736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11882_length_5736_cov_3.852843, whole genome shotgun sequence.
ACCESSION   DAOTOW010000034 DAOTOW010000000
VERSION     DAOTOW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11882_length_5736_cov_3.852843"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..569)
                     /locus_tag="P5243_01030"
     CDS             complement(147..569)
                     /locus_tag="P5243_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18056.1"
                     /translation="MNVKSMVIGAAFILVGFIVGYVIVTRAWSTVPNNGMMPPPPFQQ
                     FEGYRGHCCEKFNHGMRFFSPEFLDEIGATDEQKNQIRAILDAKRSEMEAHRKEMRAE
                     RDKVHAQIMQILTADQKALVEKRIKEGMCKREFKDSIE"
     gene            732..1505
                     /locus_tag="P5243_01035"
     CDS             732..1505
                     /locus_tag="P5243_01035"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRS18057.1"
                     /translation="MKHIFFASDFHLGYPNAAHSRERERLIIEWLTSIQSQCEELYLL
                     GDIFDFWYEYTWVVPKGYVRFLAKLAEFTDAGIPVYMFKGNHDQWLGSYLTDEIGVQI
                     LEHPIIRNENGKILYIHHGHALGPYDKGMNFLHAIFTNKVLQWCFLRIHPNGAFGFAH
                     RWSAHNRKVKVYQSANYLGDDKEWLLMYARDVLAQQHIDYFIFGHRHVAVNKKITDTS
                     TYINLGNWITNTSYAEFDGTELQLRIYKPELHPSSGIVT"
     gene            1525..2580
                     /locus_tag="P5243_01040"
     CDS             1525..2580
                     /locus_tag="P5243_01040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS18058.1"
                     /translation="MILPNDFKAEYAAIETEVQAKMHEFFAKGWYILGEEVQAFEKEF
                     ASYVGVNHCIGVANGLDALIFALKALGVGTGDEVIVPSNTYIASVLAVSHVGATPVFV
                     EPNSLTHNIDPLRIEEKITKKTKAILPVHLYGLLCEMPAIMRVAEKYNVYVVEDCAQA
                     HGATYAGKQAGSWGHVNAFSFYPTKNLGAYGDAGAVTTNDAAIAKKIQMLRNYGSQIR
                     YQNEMIGYNSRLDEIQAGLLRIKLRYLNDWNVMRRATAELFWKEFSDKAWIFPVEPQG
                     YYHVYHQFVVQSEQREADMLALESMGYKCLIHYPIPPYKSNAYKEQYEGMVFPIADEL
                     ARKVFSLPLHGTMWNKK"
     gene            2586..3509
                     /locus_tag="P5243_01045"
     CDS             2586..3509
                     /locus_tag="P5243_01045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS18059.1"
                     /translation="MYSIILLSYYSEQRIVNVFNRVSKALEQEQIPFEFIIMDDGSKD
                     NSYQVALELEKQDSRVRAYQLSKNYTSHYSKFAAFSVCKGDCATSIPDDEQMPLDIVI
                     KMYRTWQKGHKIVMPYRVSRNDGKIKDSFSNLYYKMMNSITDVTFPKGGADGFLIDRE
                     VIDILNNHISPRNTSTTVEVLRLGFDPVFIPFERPTVKNKSRWTFKKKMKLAMDTILA
                     SSTFPIKFISRLGFVSFIFSILLIILLTYIKLTGKTSIGGVSMPGWTSILAFVAFFSG
                     LILLSLGIVAEYIWKIHEEVKGRPGYIIKKK"
     gene            complement(3533..4978)
                     /locus_tag="P5243_01050"
     CDS             complement(3533..4978)
                     /locus_tag="P5243_01050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRS18060.1"
                     /translation="MKNFFNVHTFKLNTIYLYIFIISITILHAFTKFLFIGQESLYTD
                     EPYSIFYAQQSLKAIYNQCLQDSNPPLYLFILHFWIKLFGISSVAAKSLSVVANIGTA
                     LLIIAFAKKYLNTTSLIIASIIFLFSNIQFYYSHEIRTYALLGFFIVASFYTYLHVLT
                     KPTQWSLLLLGFINVAILFLHYTAIYMHIAQGISALFYFNSHRKGFWFYVYSQILSIV
                     LFLPWIHVVFANTPTGKGWLKSPNIVELKYVLYTLANNKLNFYVITIVFIIFILILLF
                     QRKYISQQFNYKIFILLTLWFIIPIFGNFFVSQYFPSFIIRTVLYSSIGLFLLIGYMI
                     SSIHYNIYIKYTIILFILFICIKQFNIKQHGPENWNKMIPKIKEIKNDNTIIIVSPWW
                     NFKAFSYYYNRSIFEDFTNTLQLLSNENIHFVNDSTNIKNIDIINYDTIILLKCRPPV
                     DNIDAYIESKGLVKNQSAGYMDLQMNMYIKP"
     gene            5213..>5736
                     /locus_tag="P5243_01055"
     CDS             5213..>5736
                     /locus_tag="P5243_01055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRS18061.1"
                     /translation="MNAYIQKSLKYLIILGLMYMPIFGHLDTLPIRIWDESRLAINSY
                     EMLQNRDYIVTHYDGKPDMWNTKPPLLIWIQVLFMKMFGVNEFSVRLPSAIAAFLTCI
                     TILFFSVRYLTNFWFGFIAVFVLMTSHGYINIHATRTGDYDALLTLFTTLSGLLFFVY
                     CEKQNYKYLYLFFL"
BASE COUNT         1993 a          786 c          985 g         1972 t
ORIGIN      
        1 aaatgccaat cagccgtatg tagtatagaa atcattgatt ttttatacaa tagtaataaa
       61 aattactgaa atggtgtata aaaaactacc cgatttgtaa agatacgtca aacaaatcgg
      121 gtagaaagta aagaaattat gcttgtttat tctatagaat ctttgaattc tcttttacac
      181 atgccttctt ttattcgttt ttcgacaagt gctttttggt cggcagttaa aatttgcatt
      241 atttgagcat gaactttatc tcgttctgct ctcatttctt ttcgatgtgc ttccatttca
      301 cttcgtttag catctaaaat tgcacgaatt tgattttttt gttcgtcggt tgctccaatt
      361 tcatctaaaa actcaggaga aaaaaagcgc ataccatggt taaatttttc acaacaatgt
      421 cctctgtaac cttcgaattg ttgaaacgga ggtggtggca tcatgccatt gttaggaact
      481 gtgctccatg ctcttgttac aattacatac cctactatga agcctactaa aataaaggca
      541 gctccaatta ccatactttt cacattcata ttcatacgtg tataatttaa attaatataa
      601 tatttattga gagctaaaat acggcagtta tacattgata ttgtactaaa atctaccaac
      661 ggttcaatta tatatacaaa ctttctgtat ctttgtgcat aataatgagt ttgtttaaca
      721 catttttttg gttgaaacat atattttttg catcggattt tcatttaggc taccccaatg
      781 cggcacatag cagagaacgt gagcgtttga ttattgaatg gcttacaagc attcaatcgc
      841 agtgcgaaga attgtatttg ttgggcgata tttttgattt ttggtatgaa tatacctggg
      901 tggttcccaa aggctatgtg cggtttcttg caaaacttgc cgaatttacc gatgccggaa
      961 taccggtata tatgttcaaa ggtaatcatg atcaatggtt gggaagttat ttaaccgacg
     1021 aaattggggt tcagatacta gagcacccta ttataagaaa tgaaaacgga aaaatattat
     1081 atattcatca tggacatgct ttgggtccat atgacaaagg catgaatttt ttgcatgcaa
     1141 tttttacaaa caaagttttg caatggtgtt ttttgcgaat tcatcctaac ggagcatttg
     1201 gatttgcaca tcggtggtcg gcacacaatc gtaaagtaaa agtatatcaa tctgccaatt
     1261 atttgggcga tgataaagaa tggcttctta tgtatgctcg tgatgtactt gctcaacaac
     1321 atattgatta ctttattttt ggacatagac atgtggctgt aaataagaag ataacagata
     1381 catcaacata tataaatttg ggtaattgga ttaccaatac aagttatgct gaatttgacg
     1441 gaacagaact acaattacgt atatataaac cggaactgca tccttcaagt gggatagtta
     1501 cataaaaaac agaattttta cggtatgata ttgccaaacg attttaaagc agaatatgct
     1561 gcaatagaaa cagaagttca agcaaaaatg cacgagtttt ttgcaaaagg atggtatatt
     1621 cttggtgaag aagtacaagc ttttgaaaaa gaatttgctt catatgttgg agtaaatcat
     1681 tgtataggag ttgcaaacgg acttgatgct ttgatttttg ctcttaaagc tttgggagtt
     1741 ggaacgggtg atgaggttat agtaccttca aatacatata tagcttctgt gttggctgtt
     1801 tctcatgtag gagcaacgcc tgtatttgtc gaaccaaatt ctcttacaca caatattgac
     1861 ccattaagaa ttgaggaaaa aataactaaa aaaactaaag caatacttcc ggttcattta
     1921 tatggattat tgtgtgaaat gcctgctatt atgcgtgtag ccgaaaaata taatgtgtat
     1981 gttgtagaag attgtgcgca agctcatgga gcaacatatg caggaaagca agccggtagt
     2041 tggggacatg taaatgcatt tagtttttat ccaactaaaa atttgggggc ttatggtgat
     2101 gccggggctg taacaaccaa cgatgcagct attgcaaaaa aaattcaaat gttgcgaaat
     2161 tatgggtctc aaattagata tcaaaatgaa atgattggct acaactcacg tttagatgag
     2221 attcaagcag gtttgctgcg tattaaatta cgatatctta atgattggaa tgttatgcga
     2281 cgagcaacag ctgaattatt ttggaaagaa ttttctgaca aagcatggat ttttccggtt
     2341 gaaccacaag gatattatca tgtatatcat cagtttgtag tacagtcaga acaacgcgaa
     2401 gccgatatgc ttgcattgga gagtatgggt tataaatgct tgatacatta tccaatacca
     2461 ccttacaaat caaatgcata caaagagcaa tatgaaggta tggtatttcc tattgccgac
     2521 gaattggctc gaaaagtttt tagtttacct ttgcatggta caatgtggaa taaaaaataa
     2581 taagtatgta ttcaattatc ttactttcat actatagtga acaacgaatt gtaaacgtgt
     2641 tcaatcgtgt aagtaaagct ttagagcaag aacagattcc gtttgagttt atcataatgg
     2701 acgacggctc taaagataat tcataccaag ttgctttaga attagaaaaa caagattcgc
     2761 gggtgcgtgc gtatcagttg agtaaaaatt atacgagtca ttattccaaa tttgcagcgt
     2821 tttctgtatg caaaggcgat tgtgctacat caattcccga cgacgagcaa atgcccctgg
     2881 atattgtaat taagatgtat cgtacatggc aaaagggaca taaaattgta atgccctatc
     2941 gcgtgagtcg taacgatggt aaaattaaag attcgttttc gaatttgtat tataaaatga
     3001 tgaattctat taccgatgtt acctttccca aaggtggtgc cgatggtttt ttgatagaca
     3061 gagaggtgat agatatacta aacaatcata tttcaccacg caatacatct accactgttg
     3121 aggtgttgcg tttaggattc gaccctgtgt ttataccatt tgaacgacct acggtaaaaa
     3181 ataaatcgcg atggacattt aaaaagaaaa tgaaattagc aatggataca attttggcat
     3241 cgtcgacatt tcctatcaaa tttatttcgc gtttgggttt tgtttcgttt attttttcta
     3301 ttttgttaat tatattgctc acatatataa agcttaccgg taaaacatct ataggaggtg
     3361 tatctatgcc cggttggaca tcgatattgg catttgttgc tttttttagt gggttgatat
     3421 tactttcgct cggaattgta gccgaatata tttggaaaat tcacgaagaa gtaaaaggaa
     3481 gacccgggta tataattaag aaaaaataga tatgttataa atttcaaata ttttagggtt
     3541 ttatatacat attcatttgt aaatccatat atcctgcaga ttgattttta actaatcctt
     3601 tagattctat atatgcatca atattatcta caggaggtct gcattttagt aatattattg
     3661 tatcatagtt tataatatca atatttttaa tattagtact atcatttaca aaatgtatat
     3721 tttcatttga taataattgt aaagtattag taaagtcttc aaatatagaa cgattataat
     3781 aatatgaaaa tgctttaaaa ttccaccaag gtgaaactat tataatagta ttatcatttt
     3841 ttatttcttt aatttttgga atcattttgt tccaattttc aggtccatgt tgtttaatat
     3901 tgaattgttt aatgcaaata aatagtataa aaagaataat tgtatactta atatatatgt
     3961 tatagtgaat cgaagaaatc atatatccta ttaataaaaa taaaccgata gaactatata
     4021 gaacagtacg tattataaaa gaaggaaaat attgtgaaac aaaaaaatta ccgaaaatcg
     4081 gaattataaa ccataatgtt aataatataa atatcttata attaaattgt tgagaaatat
     4141 attttctttg aaatagtaga ataagtataa aaataataaa aacaatagtt attacataaa
     4201 aattcaattt gttatttgca agtgtatata aaacatattt taactctaca atatttggac
     4261 tcttcaacca ccccttacct gtaggagtat ttgcaaaaac tacatgaatc caaggtaaaa
     4321 atagaacaat ggaaagaatt tgactatata cgtaaaacca aaatcctttt ctatgtgaat
     4381 taaaataaaa tagtgcagaa atgccttgtg ctatatgcat atatatcgct gtataatgaa
     4441 gaaataaaat agcaacatta atgaaaccta ataaaagtaa tgaccattga gttggttttg
     4501 taagaacatg aagataggta taaaatgaag caacaataaa aaaacctaac aaagcatacg
     4561 ttcttatctc gtgagaataa tagaattgaa tatttgaaaa taagaaaata atagaagcaa
     4621 taattaaact cgttgtattt aaatattttt ttgcaaaagc aatgattaat agagctgttc
     4681 caatattggc aacaacagaa agtgattttg cagcaaccga tgaaattcca aatagtttta
     4741 tccaaaaatg tagtataaaa agatataatg gtggattaga atcttgcaaa cattgattat
     4801 atatagcctt taaagattgc tgtgcataaa aaatactata cggttcatcg gtatataacg
     4861 attcttgacc aataaacaaa aattttgtaa atgcatgtag tattgttatg ctaattataa
     4921 atatatataa atagatagta tttaatttaa aagtatgtac attaaagaaa tttttcattt
     4981 ttttaatcta tataatttat ataaaggttt tgagagtttt tttgaaaaaa acgtatttgc
     5041 aaaatcctct taacaaagat aattataata taaatatata ttgagataat atatgttgaa
     5101 aaaggtgtaa tcttttttaa ttttgggaag ctttgtaatt aataaactaa atagtagatt
     5161 tgtataaaaa ccaataaaat tattgttcct catgcaaaga gtaatgcaaa acatgaatgc
     5221 atatatacaa aagagtttaa aatatctaat tattcttggc ttaatgtata tgccaatttt
     5281 cgggcattta gatacgttac caattaggat ttgggacgaa tctcgattgg caataaattc
     5341 atacgagatg ttacaaaaca gggattatat tgtaactcat tatgatggta aacctgatat
     5401 gtggaataca aaacctcctt tgcttatttg gattcaagta ctttttatga aaatgttcgg
     5461 tgttaacgag ttttctgttc gtttaccttc tgctatagct gcttttttga cttgcattac
     5521 aatcttgttt ttttctgtac gatatcttac aaatttttgg tttgggttta ttgcggtttt
     5581 tgtattgatg acatcgcacg gatacattaa tatacatgct acaagaacgg gggattatga
     5641 tgcgttactt actttgttta caacattgag tgggttgctt ttttttgtat attgtgaaaa
     5701 acaaaattat aagtatttgt atctcttttt tctttt
//
LOCUS       DAOTOW010000035         5717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_11916_length_5717_cov_3.064112, whole genome shotgun sequence.
ACCESSION   DAOTOW010000035 DAOTOW010000000
VERSION     DAOTOW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11916_length_5717_cov_3.064112"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="P5243_01060"
     CDS             complement(<1..1054)
                     /locus_tag="P5243_01060"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS18062.1"
                     /translation="MLQDFIKNKLSAYIPHTPTTDQRNVIHAIAQFLQSNDDILVLTG
                     YAGTGKTTLIQSIVKTLEECNIPSVQLAPTGRAAKVLCNYTQKTASTIHKKIYKQTII
                     GGFPKFQLHYNNTPKAVIIVDEASMLANQPGEQSIFGSGLLFHDLIDFVFTKTHCKLI
                     LVGDTAQLPPIGLNTSPALDVQYIQHTFLKTCHIAQLQEVVRQAEESGILQNATRIRK
                     HIDNNSIHFPKLTTTYNDTIAIQGADLLEHLEKSYNTKGIENTIVITRSNKLANRYNQ
                     GIRQNILWRDSEICAGDYIMIVKNNYYWSQHIPEIDFLANGDIVKIVAVKKYETLYNI
                     NFAQVIIECPDYNNIQF"
     gene            1146..1766
                     /locus_tag="P5243_01065"
     CDS             1146..1766
                     /locus_tag="P5243_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18063.1"
                     /translation="MNKKVKIAIELGLLGLIVVLSLLVYNSVNSNIQFEKEKLRKQKL
                     TVAKLIKIRDLQNLYEATTNKYSNSWDELINFALKDSLVFDYKEGDMEDSAAVAEGRA
                     FIKKVKRPAIEKLIADGIVANVKELKELRFVPESDEEFIIRAGMVNSGGINLQTFEVG
                     VTWDVLLKGMDKQLIINAKEASRLRSGFEGLRVGKIDEASTEGNWE"
     gene            1773..2630
                     /locus_tag="P5243_01070"
     CDS             1773..2630
                     /locus_tag="P5243_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRS18064.1"
                     /translation="MQDLLYIHKDYSNQYEQLQKVSISLRPNGFSFVIQAVDSDRILA
                     VCYSQLSANSSISVYSKSLSVFLKHEMLLKTFQTISIVYVTPRITIIPSSLFSAHNLT
                     LLFETNHVLVPNDVVLDYKLKQTQGYLVYTIPSQIIEACKEAFGNHIRFLPQAAPFLE
                     SSCIQNKIKTNKQVYILLDDTFFDIAVLEGHNILLYNTFEFSNNNDYMYFVMNVFEQL
                     QLNPLEIPVMLSGVISKASHYYESIQMFIKHVHIIEPTQSHKDFLQYPFEQSLYPFFN
                     NECNVSLCE"
     gene            2621..3163
                     /locus_tag="P5243_01075"
     CDS             2621..3163
                     /locus_tag="P5243_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRS18065.1"
                     /translation="MRIISGTFKGKQIIAPSFFTLRPTTDFAKESLCNILSNMYDFDD
                     CSLLDLFAGIGSISLEFASRGCTDIVCVEREPKHAGFIKKTVSELKIQGMHVVTNDVR
                     DFLKIAYRSFSVIFADPPYDLPWIQSIPDYIFSSKAVDDQTFVIIEHPASVSYVNHEF
                     YIKTKQYGKVHFSMFSKEKE"
     gene            complement(3167..4672)
                     /locus_tag="P5243_01080"
     CDS             complement(3167..4672)
                     /locus_tag="P5243_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRS18066.1"
                     /translation="MRIILLYICICFSFFPAFAQKRKIEIVHTDLMVGDRQVRKLYGN
                     VEFKHQDMRMFCDSAYFYVKDNKFEAYNNVKIINNDVTITSDTLYYSSQTNIAKFRGK
                     IKMVNKDVTLTTNYLDYNAKENIGSYFNNGKIVDKENILTSKIGRYTVNDNMLFFKNN
                     VVLKNKDYVIYTDTLKYQTVTKVAYFHGPTRIVSKENLLYTENGWYNTQTNIAQFFKK
                     SYINSKAQFVYGDSLYYNRNTDIGKAFKNVIIKDTVEQLFLYGQKGFYDGKADYVYVT
                     NKILLIKAFKKDSLYLHADSIFSIRLPINKAKPDSATYQIVKAYHKARFYKSDVQGVC
                     DSLLYNSLDSVIHLHIRPIIWSDQNQISGKSISLRLVNNVIEQIDIDADAFLIAQDDS
                     TQFNQMKGKKLRGYIKNNELYKVEISQNGETIYYLRDDSDLVGINKAVCNNIDAYIHN
                     GKVQKIVFKSKPSGTLYPPHEISGSATQLDNFKWLEHIRPQNHKDIYKWKE"
     gene            complement(4674..5639)
                     /locus_tag="P5243_01085"
     CDS             complement(4674..5639)
                     /locus_tag="P5243_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRS18067.1"
                     /translation="MNFTDDVAAVNSLQIAYDALKKEIHKKIVGQDAIIDDVLLSIFS
                     KGHCLLVGVPGLAKTLLVTTIAQTLGLQNNRIQFTPDLMPSDIIGTEILDSQREFKFI
                     KGPIFTNFLLADEINRTPPKTQAALLEAMQERAVTAAGIRHLLPEPFFVLATQNPIEQ
                     EGTYPLPEAQLDRFMFNVWLDYPSVDEEIEIVKTTTGTINNDIQTILTSDDILQYQQI
                     IRKIPVNDSVVRYAVELVAKTRKNTACTSPVAQEYISWGAGPRASQYLIIGAKAHAAI
                     HGKYSPDIDDVKAIALPILRHRIVKNYKAEADGLRVDDIITRLMS"
BASE COUNT         1797 a          831 c          950 g         2139 t
ORIGIN      
        1 caaattgaat attattataa tcagggcact caattataac ttgggcaaag tttatattgt
       61 ataaggtttc atattttttt actgcaacta tttttacaat atcaccattt gccaaaaaat
      121 caatttccgg tatatgttga gaccaatagt agttattttt tacaatcatt atataatcgc
      181 cggcacatat ttcggaatca cgccataaaa tgttttgacg tatgccttga ttatatctgt
      241 ttgcaagttt attgcttctt gtaattacaa tagtattttc tattcctttg gtattgtatg
      301 atttttccaa atgttcaagt aaatcggctc cttgaattgc aatagtgtca ttataggttg
      361 ttgtaagttt aggaaaatga atggaattgt tatcaatatg tttacgaata cgtgttgcgt
      421 tttgtaaaat tccggattct tcagcttgtc ttacaacttc ttgtaattga gcaatatggc
      481 atgtttttaa aaaggtgtgt tgtatgtatt gtacgtctaa tgccggacta gtatttaaac
      541 caataggagg taattgtgcc gtatcgccaa ccagtataag tttgcagtgg gttttggtaa
      601 aaacaaagtc aataagatca tgaaatagca acccgctgcc aaatatagat tgttcccctg
      661 gttgatttgc aagcatagat gcttcatcta caataataac agctttgggg gtattattat
      721 agtgtaattg aaattttgga aaaccaccta taatagtttg tttataaatt tttttgtgaa
      781 tagtacttgc agttttttgt gtgtaattgc acaatacttt tgctgctctt cctgttggag
      841 caagttgtac tgaaggtata ttacattctt ctaatgtctt tactattgat tgtattaatg
      901 tagtttttcc ggtacctgca tatcctgtaa gaacaagtat gtcatcgttt gattggagaa
      961 actgtgcaat tgcatgtatt acattacgtt ggtcggtagt aggagtatga ggtatatatg
     1021 cagataattt gttttttata aaatcttgta acatttacaa aaaaataggt tttaattatt
     1081 caactgaaaa aaaataatat tctatatttg tactttcaac aaaggtaatt taatatttaa
     1141 atacaatgaa caaaaaagtt aaaattgcaa tagagcttgg attattaggt cttatagttg
     1201 tattatctct tttagtgtat aacagtgtaa attcaaacat tcaatttgaa aaagaaaaat
     1261 tacgtaaaca aaaattgact gttgcaaaac ttattaaaat cagagattta cagaatttgt
     1321 atgaagctac tacaaataaa tattcaaact cttgggacga attaataaat tttgctttaa
     1381 aagattctct tgtgtttgat tataaagaag gagatatgga agattctgcc gcagttgcag
     1441 aaggtcgtgc ttttattaaa aaagtaaaac gtccggcaat agaaaaactg atagccgatg
     1501 gaattgttgc aaatgtaaaa gagttaaaag aattacggtt tgtgcctgaa agcgatgaag
     1561 aatttattat acgtgccgga atggttaatt caggtggtat caatcttcaa acatttgagg
     1621 ttggtgtaac atgggatgtt cttttaaaag gtatggacaa acaattgatt attaatgcaa
     1681 aagaagcttc tcgtttacgt tcagggtttg aagggttacg cgttggaaaa atagatgaag
     1741 catctacaga gggtaactgg gaataataca ttatgcaaga tttattgtat atacacaaag
     1801 attattcaaa tcaatacgaa caattacaaa aagtgtccat ttcgctgagg ccaaatggat
     1861 tttcttttgt tatacaggct gttgattctg atagaatttt agcggtatgt tattctcaat
     1921 tatctgcaaa ctcttcaata tcggtataca gtaaatcgtt atctgttttt ttaaagcatg
     1981 aaatgttgct aaaaacattt caaactattt caattgtgta tgttacgccg cgcattacaa
     2041 ttattccgtc atcgttattt tctgctcata atcttactct tttatttgaa actaaccatg
     2101 tgttagtgcc aaatgatgtg gttctagatt ataaacttaa acagacacaa gggtatttag
     2161 tatatactat tccttctcaa attattgaag cttgtaaaga agcgtttggt aatcacatac
     2221 gatttcttcc gcaagctgca ccttttttag aatcaagttg tattcaaaat aaaataaaaa
     2281 caaacaagca agtatatatt ttgcttgatg atactttttt tgatatagca gttctcgaag
     2341 gtcacaatat attattgtat aatacatttg agttttcaaa caacaatgat tatatgtatt
     2401 ttgtaatgaa tgtgtttgaa caattacaat taaatccctt agaaatcccg gttatgcttt
     2461 cgggggttat aagtaaagca tcgcattatt atgaatcaat acaaatgttt attaaacatg
     2521 ttcatataat tgaaccaact cagtctcata aagattttct tcagtatccc tttgaacaat
     2581 cgctttatcc gttttttaat aatgagtgta atgtgagttt atgcgaataa ttagtggcac
     2641 ttttaaggga aaacaaataa ttgctccctc attctttacg ttacgaccca caaccgattt
     2701 tgcaaaagaa agtttatgta atatactttc aaatatgtac gattttgatg attgttcact
     2761 gcttgatttg tttgcaggta taggtagtat tagtcttgaa tttgcctcac gaggctgtac
     2821 agacatagtt tgtgttgaac gagaacccaa acatgccggt tttattaaaa aaacggtttc
     2881 agaattgaaa attcaaggaa tgcatgtggt tacaaatgat gttcgagatt ttttaaaaat
     2941 tgcctataga agtttttcgg taattttcgc cgatccgcca tatgatttac cttggattca
     3001 atctattcct gattatattt tttcttcaaa agctgtcgat gatcaaacgt ttgttattat
     3061 tgaacatccg gcatcggttt cgtatgtaaa tcatgaattt tatataaaaa ctaaacaata
     3121 cggtaaagtg cattttagta tgtttagtaa agaaaaagaa tagttgttat tctttccatt
     3181 tataaatgtc tttgtggttt tgcgggcgaa tatgttcgag ccatttaaaa ttatctaatt
     3241 gtgttgcact gcccgaaatt tcatgtggag gatacaaggt tcccgatggt tttgatttga
     3301 aaactatttt ttgaactttt ccattatgta tatacgcatc tatattgtta catacagctt
     3361 tgtttattcc aaccaaatca gaatcgtcgc gtaaataata aattgtttct ccattttgtg
     3421 aaatttcaac tttatataat tcattatttt ttatatatcc tctcagtttt ttgcctttca
     3481 tttgattaaa ttgtgttgaa tcatcttgag ctatgagaaa agcatcggca tctatatcta
     3541 tttgttcaat tacattattt accaatcgca aagaaattga ttttccgctt atttgatttt
     3601 ggtcagacca tataatgggg cgaatatgta aatgaataac agaatctaac gaattgtaaa
     3661 gtaatgaatc acacacacct tgaacatcgg atttataaaa acgagctttg tgatatgctt
     3721 taactatttg ataggttgca gagtccggtt ttgctttatt tataggtaat cgaattgaaa
     3781 aaatagaatc ggcatgtaaa tacaaagaat cttttttaaa ggcctttatt aataaaattt
     3841 tgtttgtaac gtacacataa tcggctttgc catcataaaa tcctttttga ccataaagaa
     3901 acagttgttc aacagtatcc tttattatta catttttaaa ggccttgcca atatcggtat
     3961 tgcgattata gtataaactg tcaccatata caaattgtgc ttttgaattg atatatgatt
     4021 ttttaaagaa ttgagctatg ttagtttgtg tgttgtacca cccattttct gtatacagta
     4081 aattttcttt gcttactata cgtgtggggc catggaaata ggcaactttt gttacggttt
     4141 gatatttaag tgtgtcagta tatattacat agtctttatt tttaagtact acattgtttt
     4201 tgaaaaacaa catattatca tttactgtgt atctgccaat tttacttgtt agaatatttt
     4261 ctttatctac tattttgcca ttgttaaaat agcttccaat attttctttg gcattgtagt
     4321 caagataatt agtagtaagt gtaacatctt tgtttaccat ttttattttt cctctaaatt
     4381 tggcaatatt tgtttgactg gaatagtata acgtatcgct ggtaattgtt acatcattat
     4441 ttataatttt tacattatta tatgcttcaa atttattgtc tttaacataa aagtaggcac
     4501 tgtcacaaaa catacgcatg tcttggtgtt tgaattctac atttccataa agttttcgta
     4561 cctgtctatc ccctaccatg aggtcggtat gcacaatttc aattttgcgt ttttgagcaa
     4621 aagcagggaa aaaactaaaa caaatacata tatacaataa tataattctc atgttatgac
     4681 attaatcggg taataatatc atctacacgt aacccatctg cttctgcttt gtaatttttt
     4741 acaattctat gacgaagaat ggggagggca attgctttta cgtcatctat atcaggagaa
     4801 tatttgccgt gtattgccgc atgagctttt gctccaataa ttaaatattg tgatgctctc
     4861 ggacctgctc cccacgaaat atattcttga gcaacaggac tggtacatgc tgtgttttta
     4921 cgtgttttgg caaccaattc tactgcatat cttacaacac tgtcatttac cggaattttt
     4981 ctaattattt gttggtattg taatatatca tcactggtta gtatagtttg tatatcatta
     5041 tttatagtgc cggtagtagt ttttacaatt tctatttctt cgtcaaccga aggataatct
     5101 aaccatacat taaacataaa acggtcaagt tgtgcttcgg gtaggggata cgttccttct
     5161 tgttcaatag gattttgggt tgcaagtaca aaaaacggtt cgggtaataa atgacgaatt
     5221 ccggcagcag taacagctct ttcttgcatt gcttcaagca atgccgcttg agttttaggt
     5281 ggagttctgt taatttcgtc agcaagtaaa aagttagtaa aaatagggcc ttttataaat
     5341 ttaaattctc tttgagaatc aagtatttcg gttcctatta tatcacttgg cattaaatcg
     5401 ggtgtaaatt gtatacggtt attttgtaat cccaatgttt gagcaatagt tgtaacaaga
     5461 agtgtttttg ctaatccggg aactcctact aataaacaat gccctttgct aaaaatagag
     5521 agcaatacat catcaattat tgcatcttgt cctacaattt ttttatgtat ttcttttttt
     5581 aatgcatcat atgcaatttg caacgaattt actgctgcta catcatctgt aaaattcata
     5641 gaagtatgta ttgtattatt gttccaaaaa tacagttttt tttcggttta catactattc
     5701 cggttttaga ttttgat
//
LOCUS       DAOTOW010000036         5678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12014_length_5678_cov_3.292371, whole genome shotgun sequence.
ACCESSION   DAOTOW010000036 DAOTOW010000000
VERSION     DAOTOW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12014_length_5678_cov_3.292371"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1117
                     /locus_tag="P5243_01090"
     CDS             71..1117
                     /locus_tag="P5243_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRS18068.1"
                     /translation="MFTIFKGINLSHWLSQVFGWSPRELFITQQDIFFIKQAGFDHVR
                     LPVDEEQLWNEDFTKNKEAFADMHRCIQWCLSSDLRVVVDMHILRSHHFNNANNEGAM
                     TLWENPKEQDHMMEVWRVLSGELKQYSEDMLAYEFMNEPVAPKHHYWNELVARGHSVI
                     RELEPTRTLIFGPNLWQIPKYFESFDVPRGDANIILSFHTYDPLPFTHYKAPWIPLGA
                     YTGPVVYPGTTIPDEYLQDFIHANGDSLIAKIASEHRVFTKESMYDVIAPALKFAQER
                     GLRLYCNEFGCLPTVNRTQRLQYYTDLIDVLQLHNIAYAAWDYKGDFGIREWDKQLFS
                     NGNTDIDLISILVK"
     gene            1195..2280
                     /locus_tag="P5243_01095"
     CDS             1195..2280
                     /locus_tag="P5243_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRS18069.1"
                     /translation="MQFPQYIQNPSGFELHRGVNISHWLSQTGDWVHRPSFFTEHDVE
                     FLHSMGFDHIRLPIDEQVMFDEQGNCIDSAFISLTHAIEWCIQRNMRVIVDLHILRSH
                     HFNSRNNEGAMTLWNDTIAQNHVLNVWDSISVRLRAYPNSMVAYECMNEPVAPEHEMW
                     NKLVERMIAKIRSLEPERVIMLGSNRWQKPHTFPYLRVPKNDKNIILTVHTYHPYFVT
                     HYQAFWSAAKFYKGPVQYPGYPISAQDYASYVDTTNAALVSRIKEEKCLEYFDKQKLQ
                     NILQPAIDKAKEYGLQLYCSEFGCLPNIPREMRLRYYTDITNVFRENNISYASWDYKG
                     HFGIVTYDFESRKPTYIDTTLIQLLVK"
     gene            2307..4733
                     /locus_tag="P5243_01100"
     CDS             2307..4733
                     /locus_tag="P5243_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18070.1"
                     /translation="MKRYLYSIYIVIFVFSYSLCNANTRVITVGVFDYYPSLFKNEQG
                     EIDGYYKEAFKEIERQEKIHFVYKYCSRSECIELMELGVIDIVPNIAFDTSFAKRFIY
                     SNQNLTTIWGELYVAQNSKINTIEDITNKTIACLPHDITDMKIIELISKFGISFQLLY
                     CNSYDEVFQKVRTSQVDAGLVTNIFGAMKQEDYSVRPTDIVFNPYNIYIVGNKKHTEV
                     IQIIDSYLKKWRHDEQSIYFTARQKWLYNSFYFEKNGLPIWLFWLIGIIIFLTGVIIL
                     VVIVFRKKVQLTALQIIEKQDVIKKTESKFKTYIDNSPIAVFLINEKGEFVMVNDTTT
                     IISGYSKKELLTMNIMQLIQQEILDAGFVSFEELKTMGRIFKEVLITCKKGNKIWIEI
                     HGVKVSDTEYFCFASDIHERKMAELQILQQTEEIKQQNIELMKAKAKAEESDRLKTAF
                     LQNMSHEIRTPLNAIMGFSQLLPDYFDDKPTLEKFCNIIQDRGEDLLLMINDVLHIAR
                     IEAGQITIHHVITDLYSIKTLLIENFTEIKKRLNKTNIDLLFTIDESLLQKQIVIDDV
                     KLKQVLTNLIHNAIKFTNEGSVTVTITDKNTYLDITISDTGIGIPKEHIKNIFNRFIQ
                     IHNSGKAQLGSGLGLSIVKGLLEHVKGSIHVSSELGKGTIFFVTFPYEVPDEVKEKVE
                     IKHKEKTTLHSLSVLIVEDEITNVVFLENLLQSIVNKVFVAKDGAEAFKILANKHIDI
                     ALIDIRLPGMSGHEIAKNIRIAYPKIHIIAQTAYASEEDKEEALNAGCHDYIAKPIQR
                     NEILEKMQKI"
     gene            complement(4737..5174)
                     /locus_tag="P5243_01105"
     CDS             complement(4737..5174)
                     /locus_tag="P5243_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18071.1"
                     /translation="MIKTNLHTITLTHINLDAEGIHILAPCTIKSQNNTEAYLIIDTG
                     ASKSVFDTTYLHNNDYVEIETESIESSHITDMISGTIIKIHSICIDNRDYFDFEALSM
                     SLSHINSIYSQFVSKPVIGLIGGNFLQKYNAVISYSNMTLQLL"
     gene            5293..>5678
                     /locus_tag="P5243_01110"
     CDS             5293..>5678
                     /locus_tag="P5243_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18072.1"
                     /translation="MIAQNIIIAHESLLEYPKTVLQYTKFECGISIPDTVQQQINEFF
                     SDSNSINPAYALTSYKGINPYNAHDISVEIVFTHQSSVQIKRYGFYYQNYSYTNDSLQ
                     WIPIGNPKFLVRFSTDLIGLWTAKVI"
BASE COUNT         2004 a          754 c         1007 g         1913 t
ORIGIN      
        1 gaagcaaagg aagggggaga cgtttttcca ttaacaacgt ggtgaaacta aacacataac
       61 aataattaca atgtttacta ttttcaaagg aattaattta agtcactggc tttcgcaggt
      121 gtttggttgg tcgccacgtg aattgtttat tacccaacaa gatatttttt ttattaaaca
      181 agcggggttt gaccatgtac gtttgccggt agatgaagaa caattgtgga acgaagattt
      241 tacaaaaaat aaagaagctt ttgcggatat gcatcgttgt attcaatggt gtttatcctc
      301 tgatttgcga gttgtggtag atatgcatat tttacgttcg catcatttta ataatgccaa
      361 taatgaagga gctatgactc tgtgggaaaa ccctaaagaa caagatcata tgatggaggt
      421 gtggcgagta ttatcgggtg aacttaaaca atatagtgag gatatgcttg cctatgaatt
      481 tatgaatgaa ccggtagctc ctaaacacca ttattggaat gaattggttg ctcgcggaca
      541 tagtgttatc agagaacttg agcctacacg tactctaatt tttggaccaa atttatggca
      601 aattccaaaa tattttgaat catttgatgt ccctcgcgga gatgcaaata ttatattgag
      661 ttttcatacg tatgatccat tgccgtttac tcattataaa gctccatgga ttcctttagg
      721 tgcatataca ggccctgtag tgtacccggg tacaacaata cccgatgaat atttacaaga
      781 ttttatacat gccaatggag attctttgat tgcaaaaatt gcctcagaac acagagtttt
      841 tacaaaagag tctatgtatg atgtaattgc accggctttg aaatttgctc aagaaagagg
      901 attgcgattg tattgtaacg agtttggttg tttgcctacg gttaatagaa cgcagcgttt
      961 acaatattat acagatttaa ttgatgtact gcaattgcac aatattgctt atgcagcatg
     1021 ggattacaaa ggagattttg gtataagaga atgggataag caactgtttt caaatggaaa
     1081 tactgatata gatttaatta gtattttagt taaataggta tgataagatt tttgtatata
     1141 gttctatgta tcagtttttg ttttgtttct tgtacaaaca aaacaaaaaa aacaatgcaa
     1201 ttccctcaat atattcaaaa tcccagcgga tttgaattgc atcgtggtgt aaatataagt
     1261 cattggttat cgcaaacagg tgattgggtg catcgaccga gtttttttac cgaacacgat
     1321 gtggaatttc ttcattctat ggggtttgac catatacgat tgcctataga tgaacaagta
     1381 atgtttgatg aacaaggcaa ttgtattgat agtgcattta tatctcttac acatgccatt
     1441 gaatggtgta tacaacgaaa tatgcgagtt attgtagatt tgcatattct tcgttcgcac
     1501 cattttaatt cacgcaacaa tgaaggtgct atgactctgt ggaatgatac tattgctcaa
     1561 aatcatgtgt tgaatgtgtg ggattctata tcggttcgtc ttcgtgcata tccaaattct
     1621 atggtagcat acgaatgcat gaacgaaccc gttgctcccg agcacgaaat gtggaataag
     1681 cttgtagaac gaatgattgc aaaaattcgt tcgctcgaac ctgaacgtgt tattatgttg
     1741 ggctctaaca gatggcagaa acctcatacg tttccgtatt tgcgagtgcc taaaaatgat
     1801 aaaaatataa ttcttactgt tcatacatat catccttatt ttgttactca ttatcaagca
     1861 ttttggtcgg ctgcaaaatt ctacaaggga ccggttcaat atccgggata tcctatatca
     1921 gcacaggatt atgcatccta tgttgataca actaatgctg cattagtttc aagaattaaa
     1981 gaagaaaaat gtttggagta ttttgataaa caaaaattac aaaatatttt acaacctgct
     2041 attgataaag caaaagaata tggacttcaa ttgtattgca gtgagtttgg gtgtttgcca
     2101 aatattcctc gtgaaatgcg tctgcgttat tatactgata taactaatgt gtttagagaa
     2161 aataatatat cgtatgcaag ttgggattat aaaggccatt ttggaatagt aacatatgac
     2221 tttgaaagtc gtaaacctac atatattgat acaacactta tacaattgct tgtaaaatag
     2281 caaataataa gagtacaaaa tagattatga aacgctatct gtatagtatt tatattgtaa
     2341 tatttgtatt tagttatagc ttatgtaatg ctaatacaag agttattact gttggcgttt
     2401 ttgattatta cccatcgcta tttaaaaatg aacaaggtga aatagacggt tattataaag
     2461 aagcgtttaa agagattgag cgtcaagaaa aaattcattt tgtttataaa tactgttcgc
     2521 gttctgaatg tattgaatta atggaacttg gtgttataga tattgtacca aatattgctt
     2581 ttgatacatc atttgctaaa cgctttatat atagtaacca aaacttaaca acaatttggg
     2641 gagaattgta tgttgctcaa aattcaaaaa tcaatacaat agaagatatt accaataaaa
     2701 cgattgcttg tttgcctcat gatattacag acatgaaaat tattgaatta atttcaaaat
     2761 ttggaattag ttttcaattg ctttattgta attcgtatga tgaggtattt cagaaagtac
     2821 gtacctcgca agttgatgca gggttagtta ccaatatatt tggagcaatg aaacaagaag
     2881 attattctgt tcgtcctaca gatattgttt ttaatccata caatatatat atagttggta
     2941 ataagaagca tacagaagtc attcaaatta ttgattcgta tttaaaaaag tggcgacacg
     3001 atgaacaatc aatttatttt acagctcgtc aaaaatggtt gtataattcc ttttatttcg
     3061 aaaaaaacgg attgcctatt tggttgtttt ggttaatagg tattataata ttcttaacag
     3121 gtgttataat tttagtagtt attgtttttc gaaaaaaagt acaacttaca gcattgcaaa
     3181 taattgaaaa gcaagatgtt attaaaaaaa cagaatcaaa atttaaaaca tatatagata
     3241 attcaccaat tgcagtattc ttaataaatg aaaaaggtga atttgttatg gtaaatgata
     3301 caactactat catatcagga tatagtaaaa aagaattgct tacaatgaac attatgcaat
     3361 taattcaaca agaaatatta gatgcaggat ttgtgagttt tgaggagttg aaaacaatgg
     3421 ggaggatttt taaagaggtt ttaattacgt gtaaaaaagg taataaaatt tggatagaaa
     3481 ttcacggtgt aaaagtgagt gataccgaat acttttgttt tgcatctgat attcatgagc
     3541 gaaaaatggc ggaattgcaa attctacaac aaacagaaga aattaagcaa caaaatattg
     3601 agcttatgaa agcaaaagca aaagcagaag aaagtgatag acttaaaact gcttttttgc
     3661 aaaatatgag tcatgaaatt cgtactcctc ttaatgctat tatgggattt tctcaattat
     3721 tgcccgatta ttttgatgat aaaccaacac ttgaaaaatt ttgtaatatt atacaagata
     3781 gaggcgaaga tttgttgctt atgataaacg atgttctaca tattgctcgt attgaagcgg
     3841 gacaaattac tatacatcat gttattacag atttatattc aattaagacc ttattgattg
     3901 aaaattttac agaaattaaa aaacgcttga ataaaaccaa tatagattta ttatttacta
     3961 ttgatgagtc attattgcaa aaacaaattg taatagatga tgttaaattg aaacaagttt
     4021 tgacaaacct catacataat gctataaaat ttacaaatga aggaagtgtt actgtaacta
     4081 taaccgataa aaatacatat ttagatatta ctatttccga tacaggaata ggtattccaa
     4141 aagaacatat aaaaaatatc tttaatagat ttattcagat tcataatagt ggtaaggctc
     4201 aattaggcag tggattgggg ttaagtatag taaagggatt gcttgaacat gtaaagggat
     4261 ctattcatgt atcatctgaa ttaggaaaag gaactatatt ctttgttaca ttcccttatg
     4321 aggtgcctga tgaagttaaa gaaaaggttg aaattaaaca taaagaaaaa acaacattac
     4381 actctttatc ggtattaata gttgaagatg aaataacaaa tgttgttttt ttagagaatt
     4441 tattgcaatc tattgtaaac aaggtgtttg tggctaaaga tggggcagaa gctttcaaga
     4501 tacttgctaa taagcatatt gatatagctt taatagatat acgattaccc ggtatgtcgg
     4561 gacatgaaat tgctaaaaat attcgaattg catatcctaa aattcatata atagctcaaa
     4621 ctgcatatgc ctctgaagaa gataaagaag aagctctgaa tgcaggttgt catgattata
     4681 ttgctaaacc tattcaacga aatgaaattt tagaaaagat gcagaaaata taagcattac
     4741 aataattgta aagtcatatt tgaatatgaa attactgcat tatatttttg cagaaaattt
     4801 ccgccaataa gacctatcac aggctttgaa acaaattgag aataaataga attaatatga
     4861 gaaagagaca ttgataaagc ttcaaaatca aaataatctc tattgtcaat acatatactg
     4921 tgaattttta taatagtacc cgaaatcata tcagtaatat gtgacgactc tatactttct
     4981 gtttcaattt ctacataatc attattgtgt aaatatgttg tatcaaatac tgatttagaa
     5041 gcaccggtgt caattattag ataggcttcg gtattatttt gagatttgat agtgcaaggt
     5101 gcaagtatat gaattccttc ggcatcaaga tttatatgtg taagtgtaat agtatgcagg
     5161 tttgttttaa tcataaattc tattagtttt gaattatatt agtaaagtaa atatagaata
     5221 taattgcatg gtatgaaatt ttatgtgcgt tttatttatt ttttttgttt cagttggtta
     5281 tttgctaatc aattgatagc acaaaatatt ataattgctc atgaatcttt attagaatat
     5341 cctaaaacgg tgttgcaata cacaaagttt gaatgtggaa tttctattcc ggacactgtg
     5401 cagcaacaaa ttaatgagtt tttttctgat tctaattcaa taaatccggc atatgctctc
     5461 acctcatata agggaatcaa tccatataat gcacatgata taagtgtaga aatagtattt
     5521 actcatcaaa gtagtgttca aataaagcgg tatggatttt attatcaaaa ctattcttat
     5581 accaatgata gtttgcaatg gatacctata ggtaacccaa aatttttggt ccgtttctca
     5641 acagatttaa taggtctatg gacagcaaag gttattat
//
LOCUS       DAOTOW010000037         5666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12053_length_5666_cov_3.268401, whole genome shotgun sequence.
ACCESSION   DAOTOW010000037 DAOTOW010000000
VERSION     DAOTOW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12053_length_5666_cov_3.268401"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="P5243_01115"
     CDS             complement(<1..1504)
                     /locus_tag="P5243_01115"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18073.1"
                     /translation="MSQEYLNVSEKQAFVYAYKALELASKSNNQNYLSQAYFELGTIH
                     YKITSFDSSLYYYNKSLRTSMSNEKTALILDNIGVLYKDKSSYDSALIFHNQSLKLKE
                     TLGDRMGVAVSYTNIGNVYFQMKKFDDALDYYNQALAIRKEKQDKSLVAASLNNVGDA
                     YKNMLQYDKALACFNEALAIQKEIGNKATIANTLHYIGNFYFQLKIYDKAQEYYMKAL
                     EIRTELGNKNDIAATLFNIATVHRDIGNYREALKYYDKALTLRRETGAKEAEAHILTA
                     LGGLYKNKKLYKLAIYNYELALEMYSKMGNKQHIASSYERLGIAYKDTCLYAKAQEYY
                     TLAYSYFLQVQNMIGVARIYNYLGNLYKEKNDVPLSLQFYSRSFHLYDSLRNNVGMAQ
                     VSNNIAKLYEKIGNQANAISNYKTALHLAQQSKEYDLKQEVAFALYTIYKSNKQFAQA
                     LDYYEIYTATKDTIAHNKNVQRIAEIEFETNIKLLEQINENQDLKLREEQA"
     gene            1795..2766
                     /locus_tag="P5243_01120"
     CDS             1795..2766
                     /locus_tag="P5243_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HRS18074.1"
                     /translation="MNYKQQFTTIQSKPVLVIGDVMVDTYIWGNVDRISPEAPVPVVS
                     VTHTEHRLGGAANVVKNLSALGLQPIICTVIGNDEAGNTMRNLLLESGVSDEHCIIHN
                     QRKTTVKTRILSGNHQILRVDNEQTNSISPELTAEICKKIQKLLTSKKIAAIVFQDYD
                     KGVITKELIDFVTKFAHKYNIPIFVDPKKRNFLYYNSTTLFKPNFKEFTEGIGKQCKK
                     QDIEHVYILAKEFMLKHSIEMIMITLSEHGIFIANQHNYTHIPSQVRHVADVSGAGDT
                     VISTAIACFLAQLPIEEIARVSNIAAGLACEQPGVVTITLEQLLTHI"
     gene            2770..3930
                     /locus_tag="P5243_01125"
     CDS             2770..3930
                     /locus_tag="P5243_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS18075.1"
                     /translation="MASILIVDDERAIRNSLKDILEFEKHKVETAEHGIEGLEKLKTQ
                     KFDLVFSDIKMPHMDGIEFLKAAIEERGDIPIIMISGHATIDIAVDAIKHGAYDFISK
                     PLDLNRILITVKNALDKTSLVQETTVLKRKVLSKYEMIGNSEALTHIKEIIAKIAPTD
                     ARVLITGPNGAGKELVARAIHDQSNRKNAPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKTRIGKFEQAEGGTIFLDEIGDMSLSAQAKVLRALQEKKITRVGSDKDIQVDVRVL
                     AATNKDLKQEIANKNFREDLYHRLSVILIKVPSLAERLDDIPQLAEHFINDICKDMGV
                     PKKTISLQAIQKLQTYPWTGNIREFRNVIERLVILAGSHIELTDVEQYTQPL"
     gene            4084..>5666
                     /locus_tag="P5243_01130"
     CDS             4084..>5666
                     /locus_tag="P5243_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18076.1"
                     /translation="MKHLYTSIFTVIILIFFTSFTFSQKIIIGGTDGTIASYNPLPPT
                     PPYTLGTVGPYIDGVNKAGKIVNICSGTSISMSATTSITLNTFQWFKSVAVYNRLTRT
                     YQWSAWAAISKATDKIFTVNYETEFNSSTIYHIVYFCSITNKGGTTFTAPIGFSKALA
                     PVVQPLQVEVSYACPGQNATIIPDFDVTGDQTQVFWERELNANPGVWSEISSGSVNPF
                     YFPADTYRDGTYDYRLAIVHTCVNYRSQSLNDLGVYIAPAFNESFAALAPTSKTKTVC
                     INTQTDFTFTINGYNNFHSYTHTWEYLPNGGTTWTKITAANAGTVFRDYNTTSLKIIP
                     NAESYHNMQFRLTSVGACKTITSEVCTLLLQVPADITNITTSAETVCDGSTITLSAVI
                     NSNPLAAPVQYRWKVDNNIVPGQVFATTNNQLSFTMPNSTKTFTCEVQNGDRCIGTID
                     SYSKSIQVYSPPTVSIIPSISGCGGSTANLTAYGNGSKSPYSYEWTLPSGAKVIGQSI
                     INQPTNNTYHVKVTDACGKT"
BASE COUNT         1994 a          988 c          843 g         1841 t
ORIGIN      
        1 tagcttgttc ttcacgaagt ttcaaatcct gattttcgtt aatttgttca agtaatttaa
       61 tattcgtttc aaattcaatt tcagctatgc gttgaacatt tttattatga gctatagtat
      121 ctttagtagc tgtatatatt tcataatagt ctaaggcttg agcaaattgt ttatttgatt
      181 tataaatagt atatagagca aaagcaactt cttgttttaa atcatattct tttgattgct
      241 gagctaaatg caatgctgtt ttataattac ttatggcatt cgcttgattt ccaatttttt
      301 cgtacaattt tgcaatatta tttgaaacct gcgccatacc aacattattt cgtaacgaat
      361 catacaaatg aaatgaacga gaataaaatt gcaacgatag tggcacatca tttttttctt
      421 tatataaatt acctaaataa ttatatatac gggcaacacc tatcatgttt tgaacttgca
      481 aaaaataaga atatgccaac gtataatatt cttgagcttt tgcatacaaa caagtgtctt
      541 tatatgctat acctaaacgt tcgtatgaac ttgcaatatg ctgtttgttg cccattttag
      601 aatacatttc aagagccaat tcataattat aaattgccaa tttgtacaat tttttatttt
      661 tgtacaaacc acctaatgcg gtcaaaatat gagcttctgc ctcttttgca ccggtttctc
      721 tacgcagagt taatgcttta tcgtaatatt ttaaagcctc gcggtaatta cctatatctc
      781 tatgaacagt agcaatatta aatagcgtag ctgcaatatc atttttattc ccaagttctg
      841 tccttatttc taaggctttc atataatatt cttgtgcttt atcatatatt tttaattgaa
      901 aataaaaatt accaatataa tgaagcgtat ttgctatagt tgctttattt ccaatctctt
      961 tttgaattgc caaagcttca ttaaaacaag ctaatgcttt atcatactgt aacatatttt
     1021 tatatgcatc acctacatta ttaagagatg cagctaccaa agatttatct tgtttttctt
     1081 ttctaattgc aagagcctga ttataataat caagtgcatc atcaaatttt ttcatttgaa
     1141 agtatacatt accaatattg gtatatgaaa cagcaacgcc cattctatcg ccaagcgttt
     1201 ctttaagttt aagcgattga ttatgaaaaa ttaatgctga atcataagaa cttttatctt
     1261 tatacaacac gccaatattg tcaagaataa gcgctgtttt ttcatttgac atactggttc
     1321 tcagcgattt attgtaataa tacaatgagg aatcaaagct tgtaatttta taatgaatag
     1381 tacctaattc aaaatatgct tgtgacaaat aattttgatt attgctttta cttgccaatt
     1441 ctaatgcttt atatgcatat acaaatgctt gtttttcaga aacatttaaa tactcctgac
     1501 tcaactttga gagtatttcg atttgttgaa acccttttgt tttctgcaaa actagctgaa
     1561 ggctatcgat ttttttttca gattgagcaa tagaaaaatt acaaaaaact aatatactat
     1621 atacaaagac tactatttta cccactattg gtctatcatt cggtttcaaa acgctcaaaa
     1681 aatataagtt ttttttctct tatgaattct atgccgtgta aaactattat atttacacac
     1741 tttttgtttt tcaaatatac atataatttt aaaacaattt tactattttt tgcaatgaat
     1801 tataaacaac aatttaccac aatacaatca aaacctgtac ttgtaatagg agatgtaatg
     1861 gtagatacat atatttgggg aaatgtagat cgcatttcac cagaagctcc tgtaccggta
     1921 gtaagtgtaa ctcatacaga acaccgactt ggtggtgctg caaatgtggt aaaaaatctt
     1981 tctgctttag gactacaacc aattatttgc acagtaattg gcaatgacga agcaggcaat
     2041 actatgcgaa atttattact tgaatcggga gtaagtgatg aacattgtat tattcataat
     2101 caacgaaaaa ctaccgtaaa aactcgaatt ttatccggaa accatcaaat attgcgagtt
     2161 gacaatgaac aaacaaacag tatttcgccg gaattaactg cagaaatatg taaaaaaata
     2221 caaaaattat tgacaagcaa aaaaattgct gctattgtgt ttcaagacta tgacaaagga
     2281 gtaataacca aagaattaat tgactttgta acaaaatttg cacataaata taatattccg
     2341 atttttgttg accccaaaaa acgaaacttt ttatattata attctacaac cttgtttaaa
     2401 cctaatttca aagaatttac agaaggaatt ggcaaacaat gtaaaaaaca agatatagaa
     2461 catgtatata ttcttgccaa agaatttatg cttaaacatt ctattgaaat gattatgata
     2521 accctttctg aacatggaat tttcatagca aatcaacaca attacactca tataccaagc
     2581 caggtaaggc atgtagccga tgtttcggga gccggcgata ccgtaattag tacagcaata
     2641 gcttgctttt tggcacaatt accaatagaa gaaattgctc gtgtatcaaa tattgctgca
     2701 ggtttagctt gcgaacaacc cggcgtagta accattacac ttgaacaatt attaactcat
     2761 atataaacta tggcatctat tttaattgtt gacgacgaac gtgcaatcag aaattcgtta
     2821 aaagatattt tagaatttga aaaacataaa gtagaaactg ctgaacatgg aatagaaggt
     2881 cttgaaaaat taaaaacaca aaaatttgat ttagtgtttt ctgatataaa aatgcctcac
     2941 atggatggta tcgaattctt aaaagctgca atagaagaac gtggcgatat tcccataata
     3001 atgatttcgg ggcatgctac tattgatatt gccgtagatg ccataaaaca tggagcttat
     3061 gattttatat caaaaccatt agacttaaac agaattttaa ttaccgtaaa aaatgcactt
     3121 gacaaaactt cattagtaca agaaacaact gtactgaaac gaaaagtatt gtcaaaatat
     3181 gaaatgatag gtaattcaga agcacttaca catataaaag aaataattgc aaaaatagcc
     3241 cccacagatg ctcgtgtact aataaccggc cctaatggtg ccggaaaaga attagttgct
     3301 cgtgccattc acgatcaaag taatagaaaa aatgctccat ttgttgaagt aaattgtgca
     3361 gcaataccgt ctgaacttat tgaaagtgaa ctttttgggc acgaaaaagg tgcctttact
     3421 tcggcaatta aaactcgtat tgggaaattt gaacaagcag aaggtggcac aatctttctc
     3481 gacgaaatag gagacatgag tctttcggct caagcaaaag ttttacgagc attgcaagag
     3541 aaaaaaatta ctcgtgtagg aagcgacaaa gacatacaag ttgatgtgcg cgtattggcc
     3601 gctactaata aagacttaaa acaagagatt gccaacaaaa actttcgtga agatttgtac
     3661 caccgtttaa gcgtaattct tataaaagtt ccttctcttg ccgaaagact tgatgatata
     3721 cctcaacttg ctgaacattt cataaatgat atttgtaaag atatgggagt tcctaaaaaa
     3781 actatatcat tgcaagcaat tcaaaaatta caaacatacc cgtggacagg aaatattcgt
     3841 gaatttagaa atgttataga acgattggta attttggcag gttcacatat agagttaact
     3901 gatgtagaac aatacaccca acctttataa aaaatttttg tttttacaca aagtaacatc
     3961 agcaccacaa tacgcataca tttttatacc cattttttta tattatttat tgattttact
     4021 aagtaatata tgtacattta ccccctcaaa tcttctaaat aagaacaaca aaaaacaatg
     4081 attatgaaac acttatatac ctcaatattt accgtaatta ttctcatttt ttttacatca
     4141 tttacgtttt cacaaaaaat tataataggt ggtaccgatg gaaccattgc tagttacaat
     4201 ccgcttcctc ccactccacc ttatacctta ggcacagtag gaccatatat tgacggtgta
     4261 aataaagcag gtaaaattgt aaatatttgc agcggtacta gtatttctat gagtgctaca
     4321 acttctataa cattaaatac atttcaatgg ttcaaatctg ttgctgtata caaccgccta
     4381 accagaacat accaatggag tgcttgggct gcaatttcca aagcaacaga caaaattttt
     4441 acagtaaatt atgaaacaga atttaattcc agcacaattt atcatattgt atatttttgt
     4501 tcaattacaa acaaaggagg aacaacgttt actgctccta taggattttc aaaagcgtta
     4561 gctccggtag tacaaccttt acaagttgaa gtatcatatg cttgtcccgg acaaaatgcc
     4621 accataattc ctgattttga tgtaaccggc gaccaaaccc aagtattttg ggaacgcgaa
     4681 ctcaatgcca atcccggagt atggtctgaa ataagtagtg gttcggtaaa tccattttat
     4741 tttcctgccg atacatatag agacggtaca tatgattacc gtttagctat agttcacacg
     4801 tgtgtaaact atcgttcaca atcactcaac gatttaggag tatatatagc tcctgcattt
     4861 aatgaatcat ttgccgcact tgctcctacc agtaaaacta aaactgtatg tatcaacact
     4921 caaactgact ttacttttac tataaatggc tacaacaatt ttcactctta tacacacaca
     4981 tgggaatatt taccaaacgg tggtactaca tggacaaaaa ttactgcagc aaatgccggc
     5041 acagtattta gagattacaa cactacatct cttaaaatta tcccgaatgc cgaatcatat
     5101 cataatatgc aattcagatt aacatcggta ggagcttgta aaacaataac tagtgaagta
     5161 tgtacattat tactacaagt tccggccgat attacaaata ttacaacatc ggcagaaaca
     5221 gtatgcgatg gttctactat taccctttct gcagttataa attccaaccc actcgcagct
     5281 ccggttcaat atcgttggaa agttgacaat aatatagttc cgggacaagt ttttgcaacc
     5341 acaaacaacc aactatcttt tactatgcct aatagtacaa aaacatttac ttgtgaagtt
     5401 caaaatggag accgttgtat aggcactatt gattcttact caaaatctat tcaagtttat
     5461 tctcctccaa cagtatctat tattccaagt ataagtggtt gtggaggtag tactgctaat
     5521 cttacggctt acggaaacgg aagtaaatca ccatactctt atgaatggac tttaccaagt
     5581 ggtgcaaaag ttatcggaca atcaattata aatcaaccta ctaataatac atatcatgta
     5641 aaagtaaccg atgcttgcgg caaaac
//
LOCUS       DAOTOW010000038         5549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12339_length_5549_cov_3.381871, whole genome shotgun sequence.
ACCESSION   DAOTOW010000038 DAOTOW010000000
VERSION     DAOTOW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12339_length_5549_cov_3.381871"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="P5243_01135"
     CDS             complement(<1..628)
                     /locus_tag="P5243_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HRS18077.1"
                     /translation="MIPRVFLPMFTINELHSPFRTIFRLQNSNTEEYADILPECGGIL
                     NALYLQMPQGLYNVIDGFASDADFIANNSSSFKSNILCPFPNRIQKGTYTFENKQYTL
                     PINFESEQNAIHGLVYDKTFTVTQSYSNNTHAALHIEYSYTGDIIGFPFPFVIAIEYT
                     LSNKGLQLHTRITNTGTSPFPLGFGWHHYFRVTDSITHTALAVPTTLMY"
     gene            complement(652..1698)
                     /locus_tag="P5243_01140"
     CDS             complement(652..1698)
                     /locus_tag="P5243_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18078.1"
                     /translation="MRKKISTLIAACIASLGFAQTGSIAIVSIPDQANAGETITIQLT
                     YTSDVQVNVSAELFRTQAGSNNCDYTTWQAATTADNKAPGTNVPVSITFTIPAATTPT
                     ASLTNRNYTFQFKLIAVTGGGEFGYNNNTADNIITILPSTTVVDNIDFVTTPASSVAK
                     GGNITFDFAYTLTETRNVKAGLAIYSSTGTYLRNAEVNGSEVAQYFSDEPATTTTPVQ
                     KTASITIPAGILASNQLASGEVYKLGVSIFSTSWAYITDKKVDITITSGTDIAAIDAS
                     QLSIIPQGDVCIISAQSTINTVRINSVSGATVNIPVSIDGQQATLTTSGLAKGTYIIQ
                     AATATGTISGKLVL"
     gene            complement(1730..2842)
                     /locus_tag="P5243_01145"
     CDS             complement(1730..2842)
                     /locus_tag="P5243_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRS18079.1"
                     /translation="MKKIFFPFLILVLIGLHSCAQKQSGSLKQAYAPYFKIGTALNAS
                     HIEEGDYDGVLLVRKHFNAITPENVMKWELIHPSPTQYNFALPDAFVKFGQRDSMFMV
                     GHTLVWHSQLPQWVQSITDPVQLEQVMKEHIFQVMGRYKGKIHAWDVVNEALNEDGSM
                     RESVFYKVLGPNYIAKAFTYAHQADPNCKLYYNDYNMVVPAKRQGAIRIIKELQAQGI
                     PIHGVGMQGHWNLNWPSLQDIEQAITDFAALGITVMVTELDITVLPNPWDLVGADVNQ
                     QFQNSEKMNPYPNFLPDSVDIQLAKRYEDIFKIFIKHHTVIDRVSFWGVHDGVSWLND
                     WPIHGRTNYPLLFNRNYKPKRAFKTVMAVAKLQETK"
     gene            2960..4729
                     /locus_tag="P5243_01150"
     CDS             2960..4729
                     /locus_tag="P5243_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRS18080.1"
                     /translation="MKKLIRLLLSVMCMLLSFHVFSQNLVDNGDFEKDYDHWSRLTGN
                     NGSMTFYSLDLSNVHNGAQSMRVSTKAIGQKPWDNQLIHKGFYPTKGDEYTLSFYAKS
                     REGGVEMKAVLQNTTYNEQTYSIGTEWTKYTWEFKAAEDFLQLKFFFTALGTIFLDDI
                     TIIGKNYVPVVVRPTDTTRVNIAVKHQTIEGFGSALAFYESWITDHPSKEEMYRLAFQ
                     DLGLDWLRIRNDYKYQPDFEKAAKEFVTKAKQWRGDSIRVLMCGWTPPAELKNNNNTN
                     NGTLKKDAQGFVYEAYAKYWRDALLAYHSKGIYPDWLSIQNEPDWLTDQWETCKFEPT
                     ESAQYPGYDKAFAEVMKQFADIEKKPLMLGSEMLGIGNNQFYSYNTPIKNNPDLHAYA
                     YHLYNGGDPDFPDSYNAALQNIRKDFGNKPNVMTEYEHRKGEFYKTAWLMNNVLTQAN
                     ATAYFYWDLIWPNAGLIDIDNPYDKAGWRNPKGYQLTPHYYAYKHFTRFIHAGYTRVD
                     HTCSNPFLRASAYISPDGKQLTVVMINPTEQAIASSLNIAGVSAKKVTVYQSVANNYF
                     AVLSATPSIQLPARSVTTILYSF"
     gene            4789..>5549
                     /locus_tag="P5243_01155"
     CDS             4789..>5549
                     /locus_tag="P5243_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18081.1"
                     /translation="MLLFWAMGIYSAHAQLESSLDNLDSVKQVQKLKYSGVRFSGYTR
                     TWFFHRYLQADYELGDVGRLTNPRNISLGDGYDQPILHIQMEASPFPATKLSSEIAFD
                     NRLLRGGSFNNTVDDNGRMAAPWYLFNFTSETFTNFGSFKVTAGGGVNWFRLSPFTMG
                     SANDYNTRDDMFERLPWEGQGNDFDRYMAYYQRGDTPRDQRQGTSATQGVIVEASGLP
                     GGFNGSFLAGKASGSAGFRSYNTKKPMNMVGGRLE"
BASE COUNT         1608 a          981 c         1145 g         1815 t
ORIGIN      
        1 cgtacattag ggtggttggt actgccaatg cggtgtgagt aattgagtcg gttactctaa
       61 aataatgatg ccagccaaat cccaatggga atgggctagt acctgtattt gtaatgcgtg
      121 tatgcaactg tagtcctttg ttactcaatg tatattctat ggctataaca aatggaaacg
      181 gaaatcctat aatatcgccg gtatatgaat attctatatg caatgctgcg tgagtattgt
      241 ttgaataact ttgtgtaacg gtaaatgttt tgtcgtacac caaaccgtgt attgcatttt
      301 gctctgactc aaaatttata ggcaatgtat attgtttgtt ttcaaatgtg tatgtccctt
      361 tttgaattct attggggaac ggacataata tattgctttt gaacgaagaa ctattgttgg
      421 caataaaatc ggcatcgctt gcaaaaccgt caatcacatt gtataagcct tgtggcatct
      481 gtaaatacag tgcattgaga ataccgccac actccggcaa tatatcggca tattcttcgg
      541 tgttactatt ttgtaacctg aatatggtgc gaaatggaga atgtaattcg ttgattgtaa
      601 acataggtaa aaaaacacgg ggtatcatag caataccccg tgtatagagt gttataatac
      661 taattttcct gaaatagttc cggttgccgt agcagcttgt ataatatatg tgccttttgc
      721 taatccgctt gttgtaagtg ttgcttgttg cccgtctata gatacaggaa tatttactgt
      781 agctcctgat actgaattga tacgtactgt attaattgta ctttgtgcag aaattataca
      841 cacatcacct tgtggaatta ttgacagttg cgaggcgtct attgcagcta tatcagttcc
      901 cgatgtaatt gtaatatcaa cttttttgtc ggtaatatat gcccaacttg ttgaaaaaat
      961 agaaacgcca agtttatata cttcgcccga agctaattga ttgcttgcta atatacctgc
     1021 aggtattgta atagaagcgg ttttttgtac cggagtggtt gtagtagcag gttcatcgct
     1081 aaagtattga gctacttcgg atccgtttac ttctgcatta cgcaaatatg ttcctgttga
     1141 agagtatata gccaatcctg cttttacatt gcgtgtttcg gtaagtgtat aagcaaagtc
     1201 aaatgtaata tttccacctt ttgccacaga acttgcaggt gtagttacaa agtctatatt
     1261 atcaactacg gttgttgagg gtaaaattgt aataatatta tctgcagtat tattattgta
     1321 tccaaattca ccgcctccgg ttactgctat taatttgaat tggaatgtgt aatttctgtt
     1381 ggtaagcgaa gcggtaggag tagtagctgc aggtatagta aaggtaatag ataccggcac
     1441 attggttccc ggcgctttgt tgtcggcagt agttgctgct tgccatgtag tataatcgca
     1501 gttgttagaa cctgcttgtg ttctaaataa ttctgccgat acgtttactt gcacatcaga
     1561 tgtgtaggta agttgaatag ttatagtttc gcccgcattt gcttggtcgg gaatagaaac
     1621 tattgcaata gagcctgttt gagcaaatcc taaacttgct atacatgcgg caattagggt
     1681 actgattttt tttctcatat tcattgtatg ttttaaggtt agtacattat tattttgttt
     1741 cttgtagttt agcaaccgcc attaccgttt taaatgctcg ttttggtttg taatttctgt
     1801 taaacaaaag cgggtaattg gttctgccat gtataggcca atcgttgagc cacgatacac
     1861 catcgtgtac accccaaaat gaaactctgt ctattaccgt atggtgtttt ataaatattt
     1921 taaaaatatc ttcgtatcgt tttgccaatt gtatatctac cgaatcaggt aaaaaattag
     1981 gatacgggtt cattttttct gaattttgaa attgctggtt tacatcggca cctaccaaat
     2041 cccatggatt gggtagtaca gtaatatcta actctgttac cattacggta atgcctaatg
     2101 cggcaaagtc ggtaattgct tgttctatat cttgcaggct tggccagttg aggttccagt
     2161 gcccttgcat tcctacaccg tgaataggta tgccttgtgc ttgcagttct ttgattatgc
     2221 gaatagctcc ttgacgtttg gcaggtacta ccatattata atcgttgtaa tacaatttac
     2281 aattggggtc ggcttggtga gcataggtaa acgcttttgc tatgtaattt ggtcctaata
     2341 ctttgtaaaa taccgattcg cgcatgctgc cgtcttcgtt caatgcttcg tttactacgt
     2401 cccaagcatg tattttgcct ttgtatcgcc ccattacctg aaaaatgtgt tctttcatta
     2461 cttgctccaa ttgcaccggg tcggtaattg actgtaccca ttgtggtaac tgactatgcc
     2521 ataccaatgt atgacctacc ataaacatac tgtctctttg tccgaatttt acaaaggcat
     2581 cgggtaatgc aaaattgtat tgtgtaggcg aaggatgaat taattcccat ttcattacat
     2641 tttcgggtgt tatggcatta aagtgtttgc gtaccaacaa taccccatcg taatctcctt
     2701 cttcaatgtg tgaggcgttt aatgctgtgc caattttaaa atagggtgca tatgcttgtt
     2761 tgagcgagcc cgattgcttt tgcgcacaac tgtgtaaacc aatcaaaaca aggattaaaa
     2821 atggaaaaaa tatttttttc atatactaag tgtaaaaaat acaataaaag gtttgtttgc
     2881 aaatataatt ttttatagtt ttacatttca atattttatc aatttttttg caaaaaacat
     2941 ttactaatat aaattcatta tgaagaaact tatacgatta ttgcttagtg taatgtgcat
     3001 gttgttaagc tttcatgtgt tttcacaaaa tttggttgat aatggtgatt ttgaaaaaga
     3061 ctatgatcac tggagtagat taaccggcaa taatggttct atgacatttt attcgctcga
     3121 ccttagtaat gtgcataatg gggctcaatc tatgcgtgtt tcaaccaaag ctattgggca
     3181 aaaaccatgg gacaatcagt tgattcacaa aggattttat ccaactaaag gtgacgaata
     3241 tactctttcg ttttatgcta aatcgcgcga aggtggtgta gaaatgaagg cagtattgca
     3301 aaatactaca tataacgaac aaacctattc tattggtacc gaatggacta aatacacctg
     3361 ggaatttaaa gctgccgaag attttttgca attaaaattc ttctttacgg ctcttggaac
     3421 tatattttta gatgatatta ctattatagg taaaaattat gtgccggtgg ttgtgagacc
     3481 aaccgatact acacgagtaa atatagcggt aaaacatcaa acaattgaag gttttggttc
     3541 ggcgttggca ttctacgaaa gttggataac cgaccatcct tcaaaagaag aaatgtatcg
     3601 tttggcattt caggatttag gattggattg gcttcgcatt cgaaacgatt ataaatatca
     3661 acccgatttt gaaaaggcag caaaagaatt tgtaacaaaa gcaaaacaat ggcgaggcga
     3721 ctctatacgt gtattgatgt gcggttggac tcctcctgct gaattaaaaa ataacaataa
     3781 tacaaataac ggaacgctta aaaaagatgc tcaaggtttt gtgtacgaag catatgcaaa
     3841 atattggcgt gatgcattat tggcgtatca ttcaaaagga atttatcccg attggctgag
     3901 tattcaaaac gaacccgatt ggcttaccga ccagtgggaa acctgtaaat ttgagcctac
     3961 cgaatctgcg caatatccgg gatacgataa ggcatttgct gaggtaatga aacagtttgc
     4021 cgatatcgag aaaaaacctc tgatgttggg gagcgagatg ctggggattg gtaacaatca
     4081 gttttattca tataatactc ctatcaaaaa caatcctgat ttgcatgcct atgcatacca
     4141 tttatataat ggtggcgatc ccgattttcc cgatagctat aatgccgctt tgcaaaatat
     4201 tcgtaaagat tttggtaaca aacccaatgt aatgactgaa tatgagcacc gcaaaggtga
     4261 attttataaa actgcatggc tcatgaacaa tgtgcttacg caagctaatg ctaccgcata
     4321 tttttattgg gatttaatat ggcccaatgc aggattgata gatatagata atccgtatga
     4381 taaggcagga tggcgaaatc caaaagggta tcaacttacg cctcactatt atgcctacaa
     4441 gcattttact cgttttatac atgctggcta tacacgggta gatcatacat gttcaaatcc
     4501 gtttttacgt gcatcggctt atatttcgcc cgatggtaaa caacttaccg tggttatgat
     4561 aaatcctacc gaacaagcta tagcaagtag tttgaatatt gcaggggtgt cagctaaaaa
     4621 ggttacggtg tatcaatcgg tagcaaataa ttattttgct gtgttgtctg ccactccaag
     4681 cattcaatta cctgctcgtt cggtaactac tattttgtat tcgttttaat tatatcctat
     4741 atgaaacaca aacacttgaa gatttttagc aaaccatatg ttccttgtat gcttcttttt
     4801 tgggctatgg gaatatattc ggcacatgca caattagaat cttcgctcga taaccttgat
     4861 tcggttaagc aggtacaaaa actaaagtat agcggtgttc gcttttcggg ctacacacgt
     4921 acttggtttt ttcatcgcta tttgcaagcc gattatgaat tgggcgatgt tggacgactt
     4981 accaatccgc gaaatatttc gttgggcgat ggatacgacc agcctatttt gcatattcaa
     5041 atggaagcaa gtccatttcc ggcaactaaa ttatcgtcgg agatagcatt tgacaatcgc
     5101 ttacttcgtg gtggctcgtt caataatacg gtagatgaca atggtagaat ggctgctccc
     5161 tggtatttgt ttaattttac gtcagaaaca tttacaaatt ttggttcgtt caaagttact
     5221 gccggtgggg gtgttaattg gtttcggttg agtccgttta ctatggggtc tgctaacgat
     5281 tacaatactc gcgacgatat gtttgagcga ttaccatggg aaggtcaagg taacgatttc
     5341 gaccgctata tggcatatta tcaacgaggc gatacgccgc gcgaccaacg ccaaggaact
     5401 tcggctaccc aaggggtaat tgttgaagct tcgggcttgc cgggaggatt caatggtagt
     5461 tttcttgcag gaaaagcatc gggaagtgcc ggctttagaa gctacaacac caaaaaacct
     5521 atgaatatgg tgggtggcag attagaaaa
//
LOCUS       DAOTOW010000039         5523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12399_length_5523_cov_2.953914, whole genome shotgun sequence.
ACCESSION   DAOTOW010000039 DAOTOW010000000
VERSION     DAOTOW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12399_length_5523_cov_2.953914"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1376
                     /locus_tag="P5243_01160"
     CDS             228..1376
                     /locus_tag="P5243_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRS18082.1"
                     /translation="MRFIIFLYLWFLQLSSQGQNINIRIYSHIAIEQFQFTAANGKYD
                     IISNNSIVYTLSEGESATITFKRNKIRIEIANNHFLLDTFLLIRGVKQDNFFAISSQN
                     NQFSQRTYDNDLYVKTGSNGLVLINDVNFDKYIAGVVEAEGGSKAPIEYYKAQAVLCR
                     TYAARFYEKHIQEGFNLCDGVHCQAYKGRCKQNPTILDAAIQTSGIIMVDSTLKIASA
                     TFYANSGGETVNSEDLWSTHHYYLRSKPDPYSIGAPGYAWTKEIPASEWKSYLIQKGY
                     TVYDSIPFTFTQTQRKKYYSYNGIDTLLELKTIRNDFKLRSTFFEINHQGNMLYLQGK
                     GYGHGVGLSQEGAMEMARQNFTYDKIISFYFTGVKLMNMSSVKFFRIE"
     gene            1482..2096
                     /locus_tag="P5243_01165"
     CDS             1482..2096
                     /locus_tag="P5243_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRS18083.1"
                     /translation="MHIAIAGNIGVGKTTLARLLAKHYDWDTHYEDVDENPYLDDFYR
                     DMKAWSFNLQIFFLHSRLKQIMSIHDSGKNVIQDRTMYEDAYIFAPNLHSMDLMATRD
                     FETYFSLFRQVEQLLQPPDLLIYLKASIPSLVKRIQTKGREYENTIRIDYLESLNRRY
                     DTWAKSYDLGKMLIIDTDSINFIDNPTDLNKIINKIDAELFGLF"
     gene            2108..3139
                     /locus_tag="P5243_01170"
     CDS             2108..3139
                     /locus_tag="P5243_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRS18084.1"
                     /translation="METNERYSFNSNDLLYKIYTYRKPLLIITSLGFIISLIVSLFIT
                     PLYKSNVIVFPAPSTSISQSLITTINSYKKESVFGEVEEVEQILQVLNSDELRSKIIK
                     KYDLWKHYKIDSAKVKYPLDKMLKKFQDRINFKRTEFMAVEIEVYDPNPDTAAMIAND
                     ICMLIDTVMNRMEKERAIEAFQIVKYEYDSKVAQLNKLEDSLQFIMQKGVFDFESQSE
                     VLNKAYADAILANNTRGAQELKKQLDTLAKYGSAYISLRDFLYYEKEQLSLLNGRYKE
                     AKVDAEQILTHFYVVNKAFKSDKKAKPKRIIIVTVSTLSTFALAFVLLIFVELLQDLR
                     KKNSVQAIQ"
     gene            3177..4598
                     /locus_tag="P5243_01175"
     CDS             3177..4598
                     /locus_tag="P5243_01175"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS18085.1"
                     /translation="MYIFSALFIALNMIGIYNDTYVFSLIPFAIILGFIAFFSLDKLM
                     LILLFFVPISVELSFFIPDIPVNLSLPTEPFLILTTGMFFLKLITEKQFDKKILSHPI
                     TIAIYIYLTWLLFTSITSEMKIVSFKYLMVRIWFIVSFYLLVTQLVVNRNNISKYIWA
                     YTFGLLIAITYTVIQHADAGFTQKSANYVMYPIFRDHTSYGAALAMILPAIFILKRFS
                     KPVFNIRFMYWVVIAILIGALLLSYTRAAWLSLAAIFPIWLIVRLKIKLRTIIAVMLF
                     LVALIAPMYMDIMHNLSKNQEVSSTDFSSHIKSISNISTDASNLERINRWKCAIRMFQ
                     ERPIVGWGPGTYMFLYAPFQNSQDLTIISTNAHNNGNAHSEYLGLLAEAGFLGTLTYL
                     AILITTLIVGFKLVNSSNDDFVKQITLFLLLSLITYYLHSFLNNFLDMDKIASLFWGF
                     TAIIVAFDIEEKEKKKTHEYIKC"
     gene            4579..5295
                     /locus_tag="P5243_01180"
     CDS             4579..5295
                     /locus_tag="P5243_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRS18086.1"
                     /translation="MNTLSVEVCYSPDLFALYENKEAVVVVVDILRATSSICTAFQNG
                     VKSIIPVKTRREAMTAKENGYLVAAERNGLVLDFADFGNSPSNFSKENVYNKEIVYST
                     TNGTKTIQMAEECKAVVIGSFCNFSALYQWLINQQAPITILCAGWKGKYNIEDSIFAG
                     ALAQKILENPQYSTICDATIAAVDMWSSHNTNLRSFINKCAQHERLRSNNLDDCIDYC
                     LTFDTCNVVPIYIAGKIIRA"
     gene            5314..>5523
                     /locus_tag="P5243_01185"
     CDS             5314..>5523
                     /locus_tag="P5243_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRS18087.1"
                     /translation="MSVSKHIISQIEKKIGAVIYVQSVGGGCIAQSYTIVSIKGTYFL
                     KTNYPCAEMYKQEALGLLEIAKSNTI"
BASE COUNT         2005 a          780 c          826 g         1912 t
ORIGIN      
        1 tacatgtaaa aaatccaaaa aatatacaaa aaggagttca aactatttct ataaatggag
       61 aaacaattga ggggaatctt attcctatag acaaacttgt gaataatgca aatattgaag
      121 ttataatggg ataactgcat ttattaattt tactatagta aaaacatttg ttattgaata
      181 ttctataata aaaaactaat tactatgttt gtataaaaaa ttatagtatg cggtttatta
      241 tatttcttta tttatggttt ttgcagctaa gttctcaagg acaaaatata aatattcgca
      301 tatattctca tatagcaatt gaacaatttc agtttactgc agcaaacgga aaatatgaca
      361 taatatcaaa taactcaatt gtatatactc ttagcgaagg cgaatcggca acaattactt
      421 ttaaacgaaa taaaattaga atagaaatag caaacaatca ttttttactt gatacattct
      481 tattaatcag aggagttaaa caagataatt tttttgcaat ttcatcacaa aacaatcaat
      541 tttcgcaacg aacatatgat aatgatttat atgtaaaaac aggaagtaat ggattagtat
      601 taataaacga tgtgaatttt gataaatata tagcaggtgt tgtagaagcc gaaggcggtt
      661 ctaaagctcc aattgaatat tataaagctc aagcagtctt gtgcagaaca tatgcagctc
      721 gattttatga aaaacatatt caagaaggat ttaatttatg tgatggtgta cattgtcaag
      781 catataaagg acgatgcaaa caaaatccta caattttaga tgcagcaatt caaacatcgg
      841 gtattattat ggttgattct acattaaaaa ttgcatctgc caccttttat gcaaattcag
      901 gtggagaaac tgtaaattca gaagatttat ggtcaacaca tcattattac ttacgttcaa
      961 aaccagaccc ttatagtata ggagcaccag gatatgcatg gactaaagaa attcctgctt
     1021 ctgaatggaa atcatatctt atacaaaaag gatatactgt ttatgattct attcctttta
     1081 cttttactca aacacaacgt aaaaaatatt actcatacaa cggtattgat actttattgg
     1141 aactaaaaac aatacgaaat gattttaagc ttcgctctac atttttcgag ataaatcatc
     1201 aaggaaatat gctgtattta caaggcaaag gatatggaca tggtgttgga ttatctcaag
     1261 aaggtgccat ggaaatggca cgacaaaatt ttacatatga taaaattatt tctttttatt
     1321 ttacaggagt taagctaatg aatatgagtt ccgttaaatt ttttagaata gaataaatta
     1381 tgtgcaatat gcgtcatata catcattaaa cgttttttct caaattttag atttgcttgt
     1441 tagtattttt tgtattttag cacaaaatta aaataacata tatgcatata gctatagcag
     1501 gaaacatagg agttggaaaa accacccttg cacgtttatt ggcaaaacat tatgattggg
     1561 atactcacta tgaagatgta gatgaaaacc catatcttga tgatttttac agagatatga
     1621 aagcatggtc ttttaatttg caaatatttt ttcttcattc tcgactcaaa caaatcatga
     1681 gtattcatga ttcaggaaaa aatgtgattc aagacagaac tatgtatgaa gatgcataca
     1741 tatttgcacc taatttacat agtatggatt taatggcaac ccgagatttt gaaacatatt
     1801 tttctctttt tagacaagtt gaacaactat tgcaacctcc cgatttactt atatatttaa
     1861 aagcatctat tcccagtttg gtgaaacgaa ttcaaaccaa aggaagagaa tatgaaaata
     1921 ccattcgtat agattattta gaaagtttaa atagaagata cgacacatgg gcaaaatcgt
     1981 atgatttagg taagatgctt ataattgaca ccgattctat aaattttata gataacccaa
     2041 cagacttaaa taaaattata aataaaattg atgccgaatt gttcggttta ttttaaataa
     2101 tattcctatg gaaaccaatg agcgttattc gtttaattca aatgatttat tgtataaaat
     2161 ttatacgtat agaaaaccgt tacttattat tacttcattg ggatttatta tctcattaat
     2221 agtatcgtta tttattacac cattatacaa atcaaatgta atagttttcc ctgctccttc
     2281 tacttcaatt tcgcaatcat taattactac tattaattca tacaaaaaag aatcagtatt
     2341 tggtgaggtt gaagaagtag aacaaatatt acaggtttta aacagtgatg aattacgtag
     2401 taaaatcata aaaaaatacg atttatggaa acactacaaa atagatagtg caaaagttaa
     2461 atacccactt gataaaatgc ttaaaaaatt tcaagatcgc ataaatttta aacgaacaga
     2521 atttatggct gttgaaattg aagtgtatga tccaaatcct gataccgcag ctatgatagc
     2581 taatgatatt tgtatgctta tagatactgt aatgaatcgt atggaaaaag aacgtgctat
     2641 tgaagctttt caaatagtaa aatatgaata tgacagtaaa gtagctcaac tcaataaatt
     2701 agaagattca ctacaattta ttatgcaaaa aggcgtattt gattttgaat cacaatcaga
     2761 agttcttaat aaagcatatg ccgatgctat attagctaat aatactcgtg gtgctcaaga
     2821 actaaaaaaa caattagata ctcttgctaa atatggaagt gcatacattt ctctcagaga
     2881 ctttttatat tatgaaaaag aacaattaag tcttttaaat ggaagatata aagaagctaa
     2941 agttgatgca gaacaaattc ttactcactt ttatgttgta aacaaagcat ttaaatctga
     3001 taaaaaagca aaaccaaaac gaattattat tgtaactgtt tcaactcttt caacatttgc
     3061 attagctttt gtattactca tttttgttga attattacaa gatttgcgaa aaaagaattc
     3121 cgttcaagct atacaataga ccaacacagg tgaaaattca aaaaacatat acagttttgt
     3181 atattttttc ggcattgttt attgccttaa atatgatagg aatatacaat gatacttatg
     3241 ttttttcttt gattcctttt gcaattatct taggttttat tgcatttttt tcacttgata
     3301 aactaatgct tattttacta ttttttgtac caatttcagt agagttatct ttttttattc
     3361 ctgatattcc ggtaaattta agtttaccta cagaaccttt tttaatactt actaccggaa
     3421 tgtttttctt aaaactaatt acagaaaaac aatttgataa aaaaatatta tctcatccta
     3481 ttacaattgc catatacata tatttaacat ggctcttgtt taccagtatt acatctgaaa
     3541 tgaaaatagt atcatttaaa tatctaatgg tacgaatatg gtttatagta agtttttatc
     3601 tattagttac acagcttgtt gtaaatagaa ataatatttc aaaatatatt tgggcatata
     3661 cctttggatt acttattgct attacatata cagtcattca acatgcagat gccggtttta
     3721 ctcaaaaatc agcaaattat gtaatgtatc caatttttag agatcatact tcgtatggcg
     3781 ctgctttagc aatgatttta cctgctattt ttattctaaa acgattttca aaacctgtgt
     3841 ttaatattcg ttttatgtat tgggtagtaa ttgcaattct tataggagct ctcctcctat
     3901 catatactcg tgctgcttgg ttaagtttag ctgccatatt tccaatttgg ttaatagtac
     3961 gattaaaaat taaattacga accattattg ccgttatgtt atttttagta gcacttattg
     4021 ctcccatgta tatggatatt atgcataact tatcaaaaaa tcaagaagtt tcaagtactg
     4081 attttagttc gcatattaaa tcaatttcaa atattagtac cgatgcttcc aatttggaac
     4141 gtattaatag atggaaatgt gcaattagaa tgtttcaaga acgtccaata gttggatggg
     4201 gaccgggtac gtatatgttt ttgtatgcac cttttcaaaa ttcacaagat ttaacaatta
     4261 ttagtaccaa tgctcataat aatggtaatg cacacagtga atatttaggg cttcttgccg
     4321 aagcaggatt tttaggaact cttacatatt tagcaatact tattacaaca cttattgttg
     4381 gctttaaatt agttaattct tctaatgatg attttgttaa acaaataacg ttatttttat
     4441 tgctttcact tataacctat tatttacata gctttttaaa taatttcctt gacatggaca
     4501 aaattgcatc attattttgg ggatttaccg caataattgt tgcttttgat attgaagaaa
     4561 aagaaaaaaa gaaaacgcat gaatacatta agtgttgaag tatgttatag tcccgatttg
     4621 tttgcattgt atgaaaacaa agaggctgta gtagtggtgg tagatatttt gcgcgcaaca
     4681 agttctattt gtacagcgtt tcaaaacggt gtaaaatcta taattccggt taaaacaaga
     4741 cgagaagcta tgacagctaa agaaaatggt tatttagttg ctgccgaaag aaatggatta
     4801 gtgttagatt ttgctgattt tggtaactct ccttccaatt tttcaaaaga aaatgtatac
     4861 aacaaagaaa tagtatatag cacaacaaat ggtactaaaa ctatacaaat ggccgaagaa
     4921 tgtaaagctg tagtaattgg ttcattttgt aatttttccg cattatatca atggcttata
     4981 aatcaacaag ctcctattac aatattatgt gccggctgga aaggtaaata taacattgaa
     5041 gactcaatat ttgcaggggc tctggctcaa aaaatattag aaaacccaca atattccacc
     5101 atatgcgacg ctactattgc agctgttgac atgtggtctt cgcacaatac aaaccttcgt
     5161 tcttttatta ataaatgtgc tcaacatgaa cggttacgtt ccaataacct tgatgattgt
     5221 attgactact gtcttacttt tgatacgtgt aatgttgtac ctatttatat agcaggtaaa
     5281 atcatacggg cataattgtt ttaaactatt cttgtgagtg tatcaaaaca tataatttca
     5341 caaatagaaa agaagattgg tgcagtaatc tacgttcaat cagttggtgg tggctgtata
     5401 gcacaatcat atacaattgt atctatcaaa ggcacatatt ttttaaaaac aaattatcca
     5461 tgtgcagaaa tgtataagca agaagcatta ggattactgg aaatagcaaa atcaaatact
     5521 atc
//
LOCUS       DAOTOW010000040         5517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12420_length_5517_cov_3.993043, whole genome shotgun sequence.
ACCESSION   DAOTOW010000040 DAOTOW010000000
VERSION     DAOTOW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12420_length_5517_cov_3.993043"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="P5243_01190"
     CDS             complement(<1..560)
                     /locus_tag="P5243_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRS18088.1"
                     /translation="MNSQINNKNIILEAIRSHVPSWHYDYTPITDTSNYFIQPKEPLT
                     EWFKKTLEALSGTCIISENIEETISYIKQKTASETIYCTDNEIQTLLTQYEIQFSDSD
                     ESSRFSKFSVTHCEALVARTGSVIVSSNQITGRRPIASSEVHIVIAYAKQLYADFPQA
                     FSIIESKYQNAFPSQITSITGPSRTA"
     gene            complement(562..2475)
                     /gene="ftsH"
                     /locus_tag="P5243_01195"
     CDS             complement(562..2475)
                     /gene="ftsH"
                     /locus_tag="P5243_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRS18089.1"
                     /translation="MKPPKGNNNIFIVYAILLAAFIAINFMDLGSSSGKKTTDLEFYK
                     MLKSNEVERVVIVNKEIVEVFLKSNPGAKDVMGGAKPDYTFNIGSVESFERKVDEIQN
                     SFPDSNKISIEHQTRHNYLADIIGWILPLIILLGVWMFIMRRMGGGMGPGSQIFNIGK
                     SRAKLFDNAEDKVKTNFNDVAGLEEAKIEIREIVDFLKNPKKYTELGGKIPKGALLVG
                     PPGTGKTLLAKAVAGEAGVPFFSMSGSDFVEMFVGVGAARVRDLFKEAKAKAPCIVFI
                     DEIDAIGRARGKNPNMGSNDERENTLNQLLTEMDGFETDKGVIILAATNRADILDKAL
                     MRAGRFDRQIHVELPDMNEREAIFKVHLKPIKVSDEVKPEFLAKQTPGFSGADIANVC
                     NEAALIAARKDKKSVEKQDFLDAVDRIIGGLEKRNKIITREEKQTIAFHEAGHATLSW
                     LLEHAHPLIKVTIIPRGKALGAAWYLPEERQITTKEQIIDEICATLGGRIAEEIIFGK
                     ISTGALNDLEKVTKQAYAMVTYFGMSDKIGNVSFYDSTGQYEYAFTKPYSNETAVLID
                     KEARAIIDTAYSKARDILIKNKEGLTQLAQILLEKEVIFSEDLEKIFGKREFYSSKEH
                     KLIDEPNGTQQNS"
     gene            complement(2561..2923)
                     /gene="rsfS"
                     /locus_tag="P5243_01200"
     CDS             complement(2561..2923)
                     /gene="rsfS"
                     /locus_tag="P5243_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRS18090.1"
                     /translation="MTKPISSKKLLQTIIEGIDEKKGKNIVSLDFKKIENSICKFFVI
                     CEGDSNIHVTAISNSVIDFTQKKIGVNVWRTTGFENGEWIILDYADIMVHIFQPHIRS
                     YYAIEELWADCTIKQYTF"
     gene            complement(3077..4777)
                     /locus_tag="P5243_01205"
     CDS             complement(3077..4777)
                     /locus_tag="P5243_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18091.1"
                     /translation="MKKVQVFQLMVASAIILVVGCSSPSKMLKNASLLKYEVSPNPLE
                     MKGDSVEVTVKGSYPAKFFNKKAEMVVTPMLKWDNGSVNFGQVNLQGEGVQGNGTMIS
                     YTSGGSFTFTGKVPYQAGMLKSEMIVKSKASIKTKSVDMPDVKIADGVIATASLMKNE
                     AQTISVTNQLKRSEIISSESEILFPISQSVIQPKELTKAGMKGLKTFAKNLEKDTVLT
                     FKNVEIHGFASPDGSEAFNNNLAQGRQTAAEKAIKKELNKKEVVGNFTAEDWEGFRAL
                     MQNSNIQDKEVILSVLSRYSDPIAREAEIKKMAVVYKRIADEILPKLRRSKVVVNAEK
                     KGKTDQQIIDAALGKSTVKLTEEEFLFATSLAKSKEEEASMLKNAVATYPENWKLQNN
                     LGVVLIAQNKLQEATAALNKANELSEGDKVVKNNLGVIAVKQGDMKKAVELFEVAQGA
                     GSEVKYNLGVIKLKQGDYPAAVAMFGSNATYNASLAKLLNGDIDGALSTITKAGETDN
                     AYAYYLKAVIGARQKNVDMVVSNIKVAVEKDSTLRERAKKDLEFKAFAEDSNFKSILR
                     "
     gene            complement(5010..>5517)
                     /locus_tag="P5243_01210"
     CDS             complement(5010..>5517)
                     /locus_tag="P5243_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18092.1"
                     /translation="YYLGSVDNWINIIPKYETFNTSIEYDRTVNWTYQAIGTNMRGFN
                     QNIRNGPSFGVCNNELRLPIIQNFISRPLSSDFLANLQLIGFFDVGAAWAGLYPGAKK
                     NAYNYLIIDNTPIYIVVDEMRSPFVYGYGYGIRTRLFGYFLRLDWAYGVDEGVTQKIF
                     YLSLSLDF"
BASE COUNT         1661 a          948 c          768 g         2140 t
ORIGIN      
        1 tctgcagttc tactaggtcc tgttatgctg gttatttgcg aagggaatgc attttgatat
       61 tttgattcaa taatggagaa agcctgaggg aaatcggcat acagttgttt tgcatacgca
      121 attactatat gaacttctga agatgcaata ggtcgacgtc ctgtgatttg attagatgaa
      181 acaataacac ttcctgttct ggcaactaat gcttcacaat gagtaacaga aaatttagaa
      241 aatcgtgaag attcatctga atcagaaaat tgaatttcgt attgtgtcaa aagagtttga
      301 atttcattat ctgtacagta aatagtttct gatgctgttt tttgtttaat ataagaaata
      361 gtttcttcta tattttcaga gataatacaa gttccactaa gggcttctaa tgttttttta
      421 aaccattctg taagaggttc tttaggttga ataaaatagt ttgaagtatc agtaatagga
      481 gtataatcat aatgccatga aggtacatgt gaacgtattg cttctaaaat gatattttta
      541 ttatttattt gggaattcat attaactatt ttgctgtgtt ccgtttggtt cgtcaatgag
      601 tttatgttct ttcgatgaat aaaactcacg ttttccgaaa attttctcta aatcttcgct
      661 aaagattact tctttttcta ataggatttg agctaattgt gttaatcctt ctttattttt
      721 tataagtata tcgcgagctt ttgaatatgc agtgtctata atagcgcgag cttctttatc
      781 aattaatact gcagtttcat tactataggg tttagtaaac gcatattcat attgtcctgt
      841 tgaatcataa aaactaacat tgcctatttt atcgctcatt ccaaaataag taaccatagc
      901 ataggcctgt tttgtaactt tttccaagtc gtttaatgct ccggtagaaa ttttaccaaa
      961 tataatttct tcagcaattc ttcctcctaa agttgcacag atttcatcta taatttgctc
     1021 ttttgtagtt atttggcgtt cttcagggag ataccatgcg gcaccgagag ctttgccacg
     1081 aggtattata gttactttta taagagggtg tgcatgttcc aacaaccaac ttaatgtagc
     1141 atggcctgct tcatgaaatg caattgtttg tttttcttcg cgggttataa ttttattacg
     1201 tttttctaaa ccaccaatta ttctatcaac tgcatctaaa aaatcttgtt tttctactga
     1261 ttttttatct ttacgagcag caataagagc agcttcatta caaacatttg caatatcagc
     1321 acccgaaaat cccggagttt gttttgccaa aaattccggt tttacttcgt ctgaaacttt
     1381 aataggtttt aagtgtactt taaaaatagc ttcacgttca ttcatgtctg gtaattctac
     1441 atgaatttga cggtcaaaac gaccagcacg cataagggct ttatcaagaa tgtcggctct
     1501 atttgtagca gctaatatta taacaccttt atctgtttca aatccgtcca tttcggttaa
     1561 taattgattt agagtatttt ctctttcatc gtttgaaccc atatttggat ttttaccacg
     1621 tgcacgtcct attgcatcta tttcatcaat aaatactata caaggtgctt ttgcttttgc
     1681 ttctttgaat aaatcacgta cacgtgcagc tcctacgcct acaaacattt ctacaaagtc
     1741 tgaacctgac attgagaaaa atggaacacc tgcttcacct gcaacagctt tagccaaaag
     1801 tgttttaccg gttcccggag ggccaactaa taatgctcct ttaggaattt ttcctcctaa
     1861 ttcggtgtat tttttaggat tttttaaaaa gtcaactatt tctctaattt caatttttgc
     1921 ttcttctaat cctgcaacat cgttaaagtt agtttttact ttatcttcag cattgtcaaa
     1981 taatttcgct cgagattttc caatgttgaa tatttgcgat cccgggccca tacctcctcc
     2041 catgcgtctc ataataaaca tccaaactcc taatagaatt ataagaggta atatccatcc
     2101 aattatatct gctaaataat tgtgtcttgt ttgatgttca atactaattt tatttgagtc
     2161 aggaaaagaa ttctgaattt catctacttt tctttcaaaa gattcaacag aaccaatatt
     2221 gaatgtatag tcaggttttg ctcctcccat tacatctttg gccccaggat ttgatttaag
     2281 aaaaacttca acaatttctt tatttacaat tacaacacgt tctacttcat tagatttaag
     2341 cattttgtaa aattctaaat cagttgtttt tttaccactg cttgagccta aatccatgaa
     2401 attaatagca ataaatgctg ctaataaaat tgcatatact ataaaaatat tattattacc
     2461 tttagggggt ttcatgcctt gaaaattttg tttcccctga gaattcttag attctgattg
     2521 ttgttgattt gtttgttctt cgtttgccat tgtataaatt ctaaaatgta tattgtttta
     2581 ttgtacaatc ggcccataat tcttcaatag cataatagct tcgtatatgt ggttgaaaaa
     2641 tatgaaccat tatatctgca tagtctaaaa taatccattc tccattttca aaaccggttg
     2701 ttcgccatac gttaacacca attttttttt gagtaaaatc aattactgaa tttgaaattg
     2761 ctgtaacgtg tatgtttgaa tcaccttcgc aaataacaaa aaatttacaa attgaatttt
     2821 caattttttt aaagtccaac gaaactatat ttttcccttt tttttcgtca attccttcaa
     2881 ttattgtttg taaaagtttt ttactgctta taggttttgt catatttaaa aaacactttt
     2941 ttgacaaaaa tataaatata ttcttacaaa taaaaacaat atatattata ccattgttat
     3001 aatcaaaacc atataaaaat aaaaaaagag aaacccaact agtgagtttc tcttttattc
     3061 tatataaaat tgtatattaa cgtaagattg atttaaaatt tgaatcttca gcaaaagctt
     3121 taaattctaa atctttttta gcacgctcac gtaatgtaga atctttttca actgctactt
     3181 ttatgtttga tacaaccatg tcaacatttt tttgacgtgc accaattact gcttttaagt
     3241 agtatgcata ggcattatct gtttcacctg cttttgtaat agttgataaa gcaccgtcaa
     3301 tatctccgtt taataatttt gctaatgatg cattatatgt agcattgctg ccaaacattg
     3361 caactgcggc agggtaatct ccttgtttta attttattac acctaaatta tattttactt
     3421 cagaacctgc accttgagca acttcaaaca attcaacagc ttttttcatg tcgccttgtt
     3481 ttacagctat aacacctagg ttatttttta ctactttatc tccttctgat aattcatttg
     3541 ctttatttag tgctgctgtt gcttcttgca atttattttg tgcaattaga acaactccta
     3601 aattattttg taatttccaa ttttcaggat atgttgcaac agcatttttt aacatactag
     3661 cttcttcctc tttagatttt gctaatgatg tagcaaataa aaattcttct tctgttaatt
     3721 taacagttga tttacctaaa gcggcatcga taatttgttg gtcagtttta ccttttttct
     3781 cagcatttac tacaacttta gaacgacgta atttaggtaa aatttcatca gcaatacgtt
     3841 tataaactac tgccattttt ttgatttcag cttcacgagc tattgggtca gagtagcgtg
     3901 aaagaacaga aagaattact tctttatctt gaatgttaga attttgcatt aaagctctaa
     3961 aaccttccca gtcttcagct gtaaagttcc ctacaacctc ttttttatta agttcttttt
     4021 ttattgcttt ttctgctgct gtttgacgtc cttgagcaag attattattg aatgcttcag
     4081 aaccatcagg tgatgcaaaa ccatgaattt caacattttt gaatgttaac acagtgtctt
     4141 tttctaaatt tttagcaaat gtttttaatc ctttcattcc tgctttagta agttcttttg
     4201 gttgaattac cgattggctg attgggaata aaatttctga ttctgaagaa ataatttcgc
     4261 ttcgttttaa ttggtttgta acacttattg tttgtgcttc gtttttcatt aaactagcag
     4321 ttgcaattac tccatcagca atttttacat ccggcatatc aactgatttg gttttgatag
     4381 aagcttttga tttaactatc atttcagatt ttaacattcc tgcttggtat ggtacttttc
     4441 ctgtaaatgt gaaactaccg ccacttgtat atgatatcat agtaccattt ccttgaactc
     4501 cttcaccttg taaattaact tgtccaaagt ttacactacc attatcccat tttaacattg
     4561 gtgttacaac catttcagct tttttgttaa agaattttgc aggataactt ccttttacag
     4621 ttacttcaac actatcacct ttcatttcaa gtgggtttgg agaaacttca tattttaata
     4681 gagatgcatt ttttaacatc ttacttgggc tgctacatcc tacaactaat ataattgctg
     4741 atgctaccat taattgaaaa acttgtactt ttttcatttt cataattctt ttgaaattta
     4801 taaataacaa taaatgttaa tacgtttgtt aatgaatgac aaaaatagaa atgttttttt
     4861 atttaacaac aataaattga ttaaaaatat ttagttttta ttattttttt tactgtttgt
     4921 acacatatat ttttctatct attggcagat tttatgtatt ctaatttaaa tgcttttacc
     4981 taataaaggt atggcgtttt tgtaatacct taaaaatcaa gacttaaaga taaataaaaa
     5041 attttttgag taactccttc atctactcca tatgcccaat caagtcgcaa gaaatatcca
     5101 aacaatcgtg ttctaatacc ataaccatat ccatacacaa aagggcttct catttcatct
     5161 actactatat atataggtgt gttgtcaatg attaaatagt tataagcatt ttttttagca
     5221 ccaggataca aacctgccca tgctgctcct acatcaaaaa aacctattaa ttgtaaatta
     5281 gctaaaaagt ctgatgacaa tggtcgtgat ataaaatttt gaataattgg taatcgcaat
     5341 tcattgttac aaactccaaa cgatggcccg tttcggatgt tttgattaaa acctctcatg
     5401 tttgtgccta ttgcctgata tgtccaattt actgttcggt catattctat tgatgtgttg
     5461 aatgtttcat acttgggtat aatgtttatc caattatcaa cacttcccag ataatat
//
LOCUS       DAOTOW010000041         5517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12421_length_5517_cov_3.650494, whole genome shotgun sequence.
ACCESSION   DAOTOW010000041 DAOTOW010000000
VERSION     DAOTOW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12421_length_5517_cov_3.650494"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1371)
                     /locus_tag="P5243_01215"
     CDS             complement(73..1371)
                     /locus_tag="P5243_01215"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRS18093.1"
                     /translation="MSNILQSAQEAKQASILLAASDTVTKHKALQAIARKLQENTNNI
                     VEANTKDIERSTQQNLAAPLLKRLKFDEQKINETIAGITSLLSLPEPVGQTLMSTELD
                     TGLELYKVSCPIGVIGVIFESRPDALVQISTLCLKSGNAVLLKGGSEAIETNRILTDI
                     IRSASTESGIPQGWIQLLETRDDVNSMLKLDSYIDLIIPRGSNEFVQYIMNNSNIPVL
                     GHADGICHVYVDKDADLATAVKVTVDSKCQYVAVCNAAETLLVHSEIAEEFLPKLDIA
                     LAHHKVTLYGCERTQNIIGCKSATEEDWKTEYLDYALSIKIVDSVDEALEHINTYGSG
                     HTESIITTNSKTAEYFMNLADSGNVFWNCSTRFSDGFRYGLGAEVGISTNKIHARGPV
                     GLEGLLIYKWKLKGNGHIVGDYSAGKKTFTHKPLNKKFSI"
     gene            complement(1462..1857)
                     /locus_tag="P5243_01220"
     CDS             complement(1462..1857)
                     /locus_tag="P5243_01220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRS18094.1"
                     /translation="MEQPIFQLEFKVRDYELDLQGIVNNSVYQNYLEHARHEYLISKN
                     IDFAALHQQNIDLVVSRIEIDYKAPLKSQDKFVVKLQAAKEGNVRIVFHQWIERLPDG
                     KTMIDAKITGVCLKNGRPIRPEDATGMLW"
     gene            complement(1912..3129)
                     /locus_tag="P5243_01225"
     CDS             complement(1912..3129)
                     /locus_tag="P5243_01225"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRS18095.1"
                     /translation="MYNIQSIRNDFPILHQKIHNHDFIYFDSAATTQKPQQVIDAIVQ
                     SYSKTNSNIHRGIHTLSQVSTDLYEQARKKVQNFIHAPKAEEIIFTKGVTESINLVAY
                     SFGETFVKPGDEIIISAMEHHSNLVPWQMVCERKQAVLKFIPFNVDGILDLEWLQQSI
                     SEKTKLVSIVHVSNSLGTINPIKQIIDIAHSYNVPVCVDGAQSIQHTPINVQELDCDF
                     FAFSGHKMYAPTGIGVLWGKESWLEQMLPYQGGGDMIETVTLEKTIYNSLPFKFEAGT
                     SNYVGAHALGVAIDYIEKIGIASIQHHEQSLLMHATNKLQTIEGLTIIGNAPHKAGAI
                     SFTVQGAHPADVGLILDKKGIAIRTGTHCTEPIMQFYGIPGTARISFGMYNTIEEIDI
                     CCDILQKVTRMFL"
     gene            3328..>5517
                     /gene="metH"
                     /locus_tag="P5243_01230"
     CDS             3328..>5517
                     /gene="metH"
                     /locus_tag="P5243_01230"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRS18096.1"
                     /translation="MKRIQDILQQRILVLDGAMGTMIQKYKLTESDYRGERFKDGHIP
                     LKGNNDLLSLSKPEVIREIQRKYLEAGADILETNTFNANSISLADYNQQDLVREINIA
                     SVAIARELCNEFTQANPNKPRFVCGSIGPTNKTASMSPDVNNPAYRAVTFTDLVQVYA
                     EQVDALIDAGVDILMVETAFDTLNVKAALYAIEQTQEKKKTDVPVMVSFTIADASGRT
                     LSGQTLEACIQSVRHAKLLSIGLNCSMGAKELAPFVALLNEHCPFYVSVHPNAGLPNQ
                     FGEYDQTADIMLEMVTPFLEKSYVNIIGGCCGTTDTHIQKIASIVSNFKPRILPKPDF
                     VTRLSGLEPLYIVPEINFVNIGERTNVAGSIQFGKLIREKKYEEALSIARLQVENGAQ
                     IIDVNLDDAMLDAKEEMIHFLNLIVSEPEISRVPIMIDSSKWEVLEAGLQCVQGKCVV
                     NSLSLKEGEQEFITKAKKVKQYGAALVVMAFDEQGQADSYIRRIEICKRAYDILTKQI
                     DFPPQDIIFDPNVLTVATGIEEHNSYALDFIKSVAWIKQHLPHAKVSGGISNVSFSFR
                     GNNTVREAMHSVFLYHAIQAGLDMGIVNPGMLQVYEEIPQELREKIEAVILNTSPQAT
                     EDLIEFAETVKKTQNATQKIDAWREESVEDRLAYGLMKGIADFLEHDLTDARNIYKPT
                     LKIIEGPLMAGMNKVGDLFGQGKMFLPQVVKTARVMKKAVSILLPFIE"
BASE COUNT         1817 a          961 c          896 g         1843 t
ORIGIN      
        1 attaatactt tttttatact tttaaactgt ccggtttttg ggggagctta catatgtaac
       61 tgtaattaat ctttatattg aaaatttttt attcaaaggt ttatgcgtaa atgttttttt
      121 acctgcagaa taatctccta ctatatgtcc attaccttta agtttccatt tataaataag
      181 taatccttct aaaccaacag gacctcgagc atgaatttta ttagtactta ttccaacttc
      241 agctcccaaa ccatatctaa atccatcact aaagcgagta ctgcaattcc aaaacacatt
      301 gcccgaatca gctaaattca taaaatattc cgcagttttt gaattagttg taataataga
      361 ttctgtatgc ccgcttccat aagtatttat atgttctaaa gcttcatcta ctgaatctac
      421 aattttaata gacagagcat aatctaagta ttccgttttc caatcttctt ctgttgccga
      481 tttacatcct ataatattct gtgtacgttc acaaccatat agcgttactt tatgatgagc
      541 aagtgcaata tctaattttg gtaaaaactc ttcagctatt tcactatgta ctaagagcgt
      601 ttcggctgca ttacatacag caacatattg acattttgaa tctactgtta cttttactgc
      661 agttgctaaa tcagcatctt tgtctacata tacatggcaa attccgtctg catgacccaa
      721 tacgggaata ttggaattat tcattatgta ttgcacaaat tcattagaac ctcttggtat
      781 gattaaatct atataagaat ctaacttcaa catagaattt acatcatctc tggtttcaag
      841 taattgaatc cacccctgag gaataccgga ttcggttgag gcacttcgta ttatatctgt
      901 aagaattcta ttagtttcta tagcttcact gcctcctttg agtaacacag catttccact
      961 ttttaaacac agggttgaaa tttgaactaa tgcatcaggt cgtgactcaa atataacacc
     1021 tataactcca attggacaac ttactttata taattccaaa cctgtatcaa gttctgttga
     1081 cataagcgtt tgtccaacag gttcgggtaa tgacaataga cttgtaatac ctgctattgt
     1141 ttcattaatt ttttgttcat caaattttaa tcgcttgagc aaaggagctg ctaaattttg
     1201 ctgtgtacta cgttcaatat ccttagtatt agcttcaaca atgttatttg tattttcttg
     1261 caacttgcga gcaattgctt gtaatgcttt atgtttagtt actgtatctg atgcggcaag
     1321 caatattgat gcttgttttg cttcctgagc cgattgtaaa atatttgaca tatgtattac
     1381 ttttgagttt atgaattcaa aaatacaaaa aataaatatg caaatgctat gaaaaaaatg
     1441 tataactaca attaattgtg tttaccacaa cataccggtt gcatcttcgg gtcttattgg
     1501 tctgccattt ttaagacaaa cacctgtaat tttggcatca atcatagttt ttccatcggg
     1561 caaacgctct atccactgat gaaaaactat tcgcacattg ccttcttttg cagcttgcaa
     1621 ttttaccaca aatttatctt gacttttgag aggtgcttta taatctattt ctattcgcga
     1681 tactactaaa tcaatatttt gttggtgcaa tgctgcaaaa tcaatatttt tagaaattaa
     1741 gtactcatgg cgtgcatgtt ctaaataatt ctgatataca gagttattta ctattccttg
     1801 caaatcgagt tcatagtcac gaactttgaa ttctaattga aatattggtt gttccatgta
     1861 aattatactt tagggttagt tagactacaa aaatatttgt tttactacta tttacaaaaa
     1921 catgcgtgtt actttttgta aaatatcgca acagatatca atttcttcaa ttgtattgta
     1981 cataccaaat gaaatacggg ctgttcccgg gataccataa aattgcataa ttggttcggt
     2041 acaatgagtt cctgtacgta ttgcaatacc ttttttatcg agtataaggc caacatcggc
     2101 aggatgagct ccttgaacag taaatgaaat agcacctgct ttatgaggag catttccaat
     2161 tatggtaagt ccttctatgg tttgtagttt gttggtagcg tgcatcaaaa gagattgttc
     2221 atgatgttgt attgatgcaa tacctatttt ttcaatataa tctatagcaa cacccaaagc
     2281 atgtgctcca acataattac ttgttccggc ctcaaattta aaaggaagtg aattataaat
     2341 agttttttct aacgtaactg tttctatcat atctccacct ccttgatatg gaagcatttg
     2401 ttcaagccat gattccttac cccataacac tcctattccg gtaggagcat acattttatg
     2461 ccccgaaaaa gcaaaaaaat cacaatcaag ttcttgtaca ttaataggag tatgttgtat
     2521 agattgagcc ccatctacac acaccggcac gttatagcta tgtgctatat ctattatttg
     2581 tttaattgga tttatggttc ctaaactatt tgacacatga acaatagata ctaatttagt
     2641 cttttctgaa attgactgct gcaaccattc taaatcaaga attccatcaa cattaaacgg
     2701 tataaacttc aaaacagctt gtttacgttc acataccatt tgccatggaa ccaaattaga
     2761 atgatgttcc atagcactaa ttattatttc atcaccgggc tttacaaatg tttcaccaaa
     2821 tgaatatgca actaaattaa tagattcggt aactcctttg gtaaaaatta tttcttctgc
     2881 ttttggagca tgtataaaat tttgaacttt tttacgagct tgttcataca aatcagtaga
     2941 aacctgacta agagtatgaa ttcctctatg aatattacta ttagttttgg aatatgactg
     3001 tactatagca tctattactt gctgtggttt ttgagtagtt gcagcactat caaaatatat
     3061 aaaatcatga ttatgaattt tttgatgcaa tatcgggaaa tcgtttcgta ttgattgtat
     3121 attgtacata agtaattttt ttttgacaaa tatagacata tcatttactt tttgtgtagg
     3181 taatattcgt aaaaaaacat aattaacacc atatactatt cattttcatt aaattataat
     3241 agaatttgca tttaatttat ctacaaagtt ccaatttcat aattattata ctacatttgc
     3301 ccgtatcaat agggtaaatt agtatagatg aaacgtattc aagacattct gcaacaacga
     3361 attttggtgc tcgacggagc tatgggtacc atgattcaaa aatataaact cactgaatcc
     3421 gattatcgcg gtgaacgttt taaagacggt catattcctc ttaaaggaaa caatgattta
     3481 ttaagcctgt caaaaccaga agttattcgc gaaattcagc gaaaatacct tgaagcaggt
     3541 gccgatattc ttgaaacaaa tacttttaat gccaatagta tatcgcttgc tgattataat
     3601 caacaagatt tagtacgcga aataaatata gcttcagttg ctattgcacg tgaattgtgt
     3661 aatgaattta cccaagcaaa tcctaacaaa ccacgctttg tttgcggttc tataggccct
     3721 accaacaaaa cagcttcaat gtcgcccgat gtaaataacc ctgcatatag agcagttaca
     3781 tttacagatt tagttcaagt atatgcagaa caagttgatg cattaataga cgcaggtgta
     3841 gatatactta tggtagaaac agcattcgac accttaaatg taaaagcagc actgtacgca
     3901 atagaacaaa cacaagaaaa gaaaaaaact gatgtgccgg taatggtttc atttacaata
     3961 gccgatgcaa gtggtagaac tttatccggt caaacactag aagcgtgtat acaatcggtt
     4021 cgtcatgcta aattattaag cattgggctt aattgttcaa tgggagccaa agaattagct
     4081 ccttttgtag ctttgcttaa tgaacattgt cctttttatg taagcgttca tcctaatgcc
     4141 ggattaccaa atcaatttgg tgaatacgac caaactgcgg atataatgct tgaaatggtt
     4201 acgccatttc ttgaaaaatc atatgttaat ataataggag gctgttgcgg aactaccgat
     4261 acacatatac aaaaaattgc ttcaattgtt tctaatttca aaccacgaat attgccaaaa
     4321 cccgattttg ttactcgctt aagcgggcta gaaccattgt atatagtgcc cgaaataaat
     4381 tttgtaaaca ttggcgaacg taccaatgta gcaggttcta tacaatttgg caaactgatt
     4441 cgtgaaaaaa aatatgaaga agcattgtct attgctcggt tacaagtaga aaacggtgct
     4501 caaataatag acgtaaattt agacgatgca atgcttgatg caaaagaaga aatgattcat
     4561 ttcttaaatc ttattgtatc ggaacccgaa atttcaagag tacccataat gatagattct
     4621 tctaaatggg aagtacttga agcaggatta caatgtgttc aaggtaaatg cgtagtaaat
     4681 tcattaagtt taaaagaagg tgaacaagaa tttataacaa aagcaaaaaa agtaaaacaa
     4741 tatggtgctg cacttgttgt aatggcattt gatgaacaag gacaagccga ttcatatatt
     4801 cgcagaattg agatttgtaa acgagcatat gatatattaa ccaaacaaat tgactttcct
     4861 ccacaagaca taatttttga ccctaacgta ttaactgtag caacaggtat tgaagaacat
     4921 aattcatatg ccttagattt tataaaatct gtagcatgga ttaaacaaca tttaccccat
     4981 gcaaaagtaa gtggtggcat aagtaatgtg tcgttttcat ttagaggtaa caataccgta
     5041 cgcgaagcta tgcattctgt atttttgtat catgctatac aagccgggct cgatatgggt
     5101 atagtaaacc ccggtatgtt gcaagtgtac gaagaaattc ctcaagaatt acgagaaaaa
     5161 attgaagctg taatccttaa tacgtcacca caagctactg aagacttaat agaatttgcc
     5221 gaaactgtta aaaaaacgca gaatgctact caaaaaattg acgcttggcg tgaagaatct
     5281 gtagaagaca gattggctta tggcctcatg aaaggcatag ccgatttttt ggaacatgat
     5341 ttaacagatg cgcgcaacat ttacaaaccc actctcaaaa ttattgaagg gccgcttatg
     5401 gcaggcatga ataaagtagg tgatttattt gggcaaggaa aaatgttttt gcctcaggtt
     5461 gtaaagaccg ctcgtgttat gaagaaggct gtttctatat tactgccatt tattgaa
//
LOCUS       DAOTOW010000042         5409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12714_length_5409_cov_4.198730, whole genome shotgun sequence.
ACCESSION   DAOTOW010000042 DAOTOW010000000
VERSION     DAOTOW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12714_length_5409_cov_4.198730"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="P5243_01235"
     CDS             complement(<1..388)
                     /locus_tag="P5243_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18097.1"
                     /translation="MKLIRVYILCMLLVPTMVYAQLFVANQATLHIDANTTFFIPQNI
                     ENEGIISNQGTIEIIGNWINPGTNTPTGTVIFEGTNQQIHQYGNTFYNVLITGGGNKQ
                     VPSSMYILNSIEFDNGYLLPIDTAKII"
     gene            complement(400..4377)
                     /locus_tag="P5243_01240"
     CDS             complement(400..4377)
                     /locus_tag="P5243_01240"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRS18098.1"
                     /translation="MKILYNLRKLILTSFIAGFLSIAAYTQSSVGINTTNPNPNAVLE
                     LVSPNNNQGLKIPSLTTAQRTNAAFVNNLTAADKGLIVFDSDIASFFYWSGSDWTKIS
                     KGEIPTLEEILAVSNSAASKKISDLANPENAQDAATKKYIDDSLTVVNQKISIINDSI
                     TNFDTKITNLKTDVTNLDNRVTNTEGAISSLQTNITNLDNRLTTTENNVTTLQTNVSN
                     LQTNVSNLQTNVTNLDNRVTTTENNISTLQTNVSNLQTDVTNLQTDVTNIDNRLTTTE
                     NNVTTLQTDVTNLDNRVTTTENSISNLQTDVTNLENNVTTLQTDITNLISNPELTLAE
                     DKIFVGNASGKTNAVDMTGAITINSTGATTLTNGIVTAPKLAGDNIGTPLTNGTVDWV
                     LTSKGDGSFKWTNPSTTPVDPNNITLLNGNIFVGNASDKAAGVTMQGDATIINNGTIT
                     IANNAITSAKIANGTIANIDLDKANIPLSGFGAATTDVSLGSNKLINVADPTLAQDAA
                     TKNYVDTKATSDLAGKANVNQTFYLGTTNIAINRASGSQSLTGITSIDGSAATLTTAR
                     LINGTSFNGSANITVPLNTTTDNANAATMYPVWTTGTGNNQASISTTKLSFVPSTGIL
                     TATGFSGSGAGLTNIPNAALSNSSITIGTTAINLGASSTTLAGLTSVTSTSFTGALTG
                     NATTATTATNIAGGVGGSIPYQTAANTTALLANGTVGQVLTSAGGTNPPTWSSISVPT
                     GANPTASIGLTATNGVATTFMRSDAAPALDVAITPTWTGAHIWSALGTFNAGLNASGA
                     AVNLNANSNNAVNIGTGTSTGTVTIGGTAAQTIAIGNGAAAKTVTLGSTNTTSTTTIN
                     AGTGNILLSAPSTTVQTNTATDDQIRILPYTGGAARFAGTVTSADLTAARTWTLPDAT
                     GTLALTTDIPTVPTAANPTASIGLTAINGSATTFMRSDAAPALDVAITPTWTGAHIWS
                     ALGTFNAGLNASGAAVNLNANSNNAVNIGTGTSTGTVTIGGTAAQTIAIGNGAAAKTV
                     TLGSTNTTSTTTINAGTGGINLTTGAATSMITTLGTTGAAEFRSSTANSDRIRILPQS
                     TAVGGTFTGTLTSADLTAARTWTLPDADGTFLMGSGLTTNTVPRWNGSALGNSSIWDN
                     GSGNVHIGNSTISASYALQVTGAFKTEKIYHSSDARWKQNVLPIDNALSKVQQLRGVT
                     YEWKTEEFPEKHFSKGTQIGLIAQEVEKVLPELVITDDTGYKAVEYANIVAVLIEAIK
                     EQQAIIDAQKLEIEKLQVSNESMKQEITQLQTMQTSIESMQKQINSMQALLQILQPTA
                     VQTQK"
     gene            complement(4439..>5409)
                     /gene="mnmA"
                     /locus_tag="P5243_01245"
     CDS             complement(4439..>5409)
                     /gene="mnmA"
                     /locus_tag="P5243_01245"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRS18099.1"
                     /translation="QAFAKKIGIPHHIVDVRNSFKDIVVTDFINEYIAGRTPNPCVVC
                     NPAIKWGEVIKKAEELGCDFIATGHYSQIAQLNGRYYFTKAADTSKDQSYVLWKLPQE
                     FIKKTLLPLGKYKKSEIKQLAMERGFEKLAKKRESQEICFIPDDDYRNFLKKRVPHLE
                     SSVNGGEFISTTGKHLGTHKGYPFYTIGQRKGLNIALGKPTYVVAIKKDTNQIILGDK
                     GDLLQEKMIVSHINFLKYSQFDENSLYSVKIRYNSTPNICKIRTKDIDTIEVEFVNSV
                     GGITPGQSAVFYEGDDVVAGGIIQYKQINTFVFCYKIVNKLFVNRL"
BASE COUNT         1571 a          898 c         1014 g         1926 t
ORIGIN      
        1 gaattatttt agctgtatct ataggaagta aatatccgtt atcaaattct attgaattga
       61 gtatatacat acttgaagga acttgtttgt ttccgccacc tgttataagt acattgtaaa
      121 atgtatttcc atattgatga atttgttggt ttgttccttc aaaaatcaca gtcccggttg
      181 gggtattagt tcccgggttt atccaatttc caatgatttc tattgtcccc tgattgctta
      241 ttatgccttc attttcaata ttctgaggga taaaaaaagt tgtgtttgca tcaatatgca
      301 aagtagcttg atttgcaaca aataactgtg catataccat tgttggtaca agtagcatac
      361 acaatatata tacgcgaatc aatttcataa acaatgttgt tatttttgag tttgaacagc
      421 tgttggttgt agtatttgta gtaatgcttg catagaattt atttgttttt gcatagattc
      481 tatactagtt tgcatagttt gtaattgtgt aatttcttgt ttcattgatt cgtttgaaac
      541 ttgtagtttt tcaatttcta atttttgagc atcaatgata gcttgttgtt ctttaattgc
      601 ttcaatcaag actgcaacta tattagcata ttctactgct ttataaccgg tgtcatcagt
      661 aattactaat tcaggtaaaa ctttttcaac ttcttgggct attaaaccaa tttgggttcc
      721 tttgctaaaa tgtttttcgg ggaattcttc ggttttccat tcataggtaa caccacgtag
      781 ttgttgtact tttgataatg cattatcaat tggtaaaaca ttttgtttcc atcgagcatc
      841 tgatgaatga taaatttttt cggttttgaa tgctccggta acttgtaatg cataacttgc
      901 actaatagtg ctatttccaa tgtgaacatt tcctgaacca ttatcccaaa tagagctatt
      961 acctaatgcg gaaccattcc aacgcggaac agtattggta gtaagaccgg aacccattaa
     1021 gaatgtgcca tcggcatcag gtaatgtcca tgtgcgagca gcagtaagat cagctgatgt
     1081 aagagtacct gtaaaagtac cacctacagc agtagattgt ggtagaattc taattctgtc
     1141 ggaattagct gttgaagaac ggaattcagc tgcgccggtt gtacctaatg ttgtaatcat
     1201 actagtagca gcaccggtag taagattaat acctcctgtg cctgcattaa ttgtagtagt
     1261 acttgtggta ttagtactac caagagtaac agttttagcc gcagcgccgt taccaatagc
     1321 aatagtttga gcggcagtgc caccaatagt aacagtaccg gtagaggttc ctgtaccaat
     1381 atttacggca ttgtttgaat ttgcatttaa gttaacagca gcgccagaag cgtttaaacc
     1441 tgcattaaaa gtacctaaag cactccatat atgagctccg gtccatgtag gagtaatagc
     1501 aacgtcaaga gcaggggcag catcgctacg cataaatgtg gtagcagaac cattaatagc
     1561 agtaagtcca atacttgctg taggatttgc tgcagtaggt acagtaggaa tatcggttgt
     1621 gagtgctaaa gtacctgttg catcaggtaa tgtccatgtg cgagcagcag taagatcggc
     1681 agaggtaaca gtacctgcaa aacgagcagc accgccggta tatggaagaa ttctaatttg
     1741 gtcgtcggta gcagtattag tttgtacagt agtagaagga gccgaaagta aaatattacc
     1801 tgtaccggca ttaattgtag tagtacttgt agtattggta ctaccaagag taacagtttt
     1861 agccgcagcg ccgttaccaa tagcaatagt ttgagcggca gtgccaccaa tagtaacagt
     1921 accggtagag gttcctgtac caatatttac ggcattgttt gaatttgcat ttaagttaac
     1981 agcagcgcca gaagcgttta aacctgcatt aaaagtacct aaagcactcc atatatgagc
     2041 tccggtccat gtaggagtaa tagcaacgtc aagagcaggg gcagcatcac tacgcataaa
     2101 tgtagtagct acaccattgg tagcagtaag accaatacta gctgtaggat ttgcacctgt
     2161 tggaacagaa atcgaactcc atgtaggagg gttagtgccg ccagccgatg ttaatacttg
     2221 accaacggtg ccatttgcta ataatgctgt agtatttgct gcggtttgat atggaatact
     2281 tcctccaaca ccacctgcaa tatttgtagc agtagttgct gtagtagcat ttccagtcaa
     2341 tgctccggta aacgatgttg aagttaccga tgttaatcct gctaaagttg ttgaacttgc
     2401 acctaaattt attgcagtgg ttcctatagt tatacttgaa ttggaaagag cagcatttgg
     2461 tatatttgtt aaacctgcac cggaacctga aaatcctgtt gccgttaaaa ttccggtaga
     2521 aggaacaaaa cttaattttg tagttgaaat gctcgcttga ttatttcctg tgcctgttgt
     2581 ccataccgga tacatagttg ctgcatttgc attgtctgta gtagtgttca aaggaactgt
     2641 aatatttgca gaaccattaa atgaagttcc attaattaaa cgagctgtag taagtgttgc
     2701 tgcactacca tcaatactgg taatacctgt taatgattgt gatcccgatg ctctattgat
     2761 agctatattt gtagttccta aataaaaagt ttgattcaca ttagctttac ctgctaaatc
     2821 tgatgttgct ttggtgtcaa cataattttt cgttgctgca tcttgtgcaa gtgtaggatc
     2881 agctacgttt attagtttat tcgatccaag agatacatct gttgttgctg caccaaaacc
     2941 acttagtgga atattagctt tatctaaatc aatatttgca atagttccat ttgctatttt
     3001 tgccgatgtt attgcattgt ttgctattgt aatagttccg ttatttataa ttgttgcatc
     3061 gccttgcatt gttacaccgg cggctttatc tgaagcattt ccaacaaaaa tatttccatt
     3121 aagtaaggtt atattgttag ggtcaactgg agttgtactt ggattagtcc atttaaaact
     3181 cccatcacct tttgatgtaa gaacccaatc aacagtacca tttgttaatg gagtaccgat
     3241 attgtctcct gctaattttg gagctgttac tataccattt gttaaagttg tagctcctgt
     3301 agaatttatt gtaattgctc ctgtcatatc aacagcattt gttttgccgc tggcattacc
     3361 aacaaatatt ttatcctcag ctaaagttaa ttcaggattt gaaattaagt ttgttatatc
     3421 tgtttgaagc gttgtaacat tattttctag atttgtaacg tcagtttgga gatttgagat
     3481 actattttca gttgtagtaa ctctattatc gaggttagta acatcagttt gcagagtagt
     3541 aacattgttt tctgtagtag taagtcggtt gtcaatatta gttacatcgg tttgtaaatt
     3601 ggtgacatcg gtttgcaggt tacttacatt agtttgcaga gttgatatat tattttcagt
     3661 tgtagtaact ctattatcga ggtttgttac attagtttgt aaattactaa cattggtttg
     3721 taggttactc acattagttt gcagagtagt aacgttgttt tcggtagtag taagtctgtt
     3781 atcaaggtta gtaatattag tttgcaaaga agaaattgca ccttctgtat tcgttacacg
     3841 attgtctaaa ttagttacat cggttttaag attggtaatt tttgtgtcaa aatttgttat
     3901 actgtcattt attatggaga ttttttggtt tacaactgtt aatgagtcat caatatattt
     3961 tttagttgca gcgtcttgag cattttcagg attagctaaa tcgcttattt ttttgctggc
     4021 agcactattt gaaacagcta aaatttcttc gagtgttgga atttcacctt tagaaatttt
     4081 tgtccaatct gaacctgacc aataaaagaa agatgcaata tcactatcaa atacaattaa
     4141 acccttgtca gcagcagtta aattattcac aaaagcggca tttgttcttt gcgctgttgt
     4201 aagtgatggt atttttaacc cttgattatt atttggagaa actaattcta aaactgcatt
     4261 tgggttagga ttagttgtat taatacctac agagctttgt gtgtaagccg caatcgataa
     4321 aaaaccggct ataaagcttg ttaatattag ttttcggagg ttgtatagta ttttcatact
     4381 gattatattt ttaaattttt gtgctcgtat gtgataaaac tttgcaaaag taaaacaatt
     4441 ataatctatt aacaaacaat ttattaacta ttttataaca aaaaacaaat gtgtttattt
     4501 gtttgtattg tattatgcct cctgccacca catcatcgcc ttcgtaaaaa accgcagact
     4561 gaccaggtgt aattccgcca actgaattta caaattctac ttctattgta tctatgtctt
     4621 ttgtacgaat tttacaaata ttaggtgtcg aattatatcg tatttttacg gaatacaatg
     4681 aattttcatc aaattgtgaa tatttgagga aattaatgtg agaaacgatc attttttctt
     4741 gtaacaaatc acctttatct cctagaatta tttggtttgt gtctttttta atagctacaa
     4801 catatgttgg ttttcctaaa gctatattaa gaccttttct ttgccctata gtataaaaag
     4861 gataaccttt gtgtgtacct aaatgttttc ctgtggttga aataaattcg ccaccattta
     4921 ctgaagattc aagatgaggc acacgttttt ttagaaagtt tctataatcg tcgtcaggaa
     4981 taaagcaaat ttcttgactt tcacgttttt ttgcaagttt ttcgaatcct cgttccattg
     5041 ctaattgttt aatttcactt tttttgtatt ttcctaatgg aagtaatgtt tttttaatga
     5101 attcttgggg taatttccac agtacatatg attgatcttt tgatgtgtca gcagcttttg
     5161 taaaataata tctaccattt aattgcgcaa tttgagaata atgtccggta gcaataaaat
     5221 cacaaccaag ttcttcggct tttttaatta cttcgcccca tttaatagca ggattgcata
     5281 caacacaagg atttggtgtt cgaccggcta tatattcgtt tataaaatca gtaacaacta
     5341 tgtctttaaa tgaattacga acatctacta tatggtgagg aataccaatt ttttttgcaa
     5401 aagcttgtg
//
LOCUS       DAOTOW010000043         5389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12775_length_5389_cov_3.179228, whole genome shotgun sequence.
ACCESSION   DAOTOW010000043 DAOTOW010000000
VERSION     DAOTOW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12775_length_5389_cov_3.179228"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="P5243_01250"
     CDS             complement(<1..1248)
                     /locus_tag="P5243_01250"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRS18100.1"
                     /translation="MDILSLFVVVPILTMIGILFTKDLKGARMVSAIGMGIQLLMSFN
                     LLFQYIAERKAGNTAEMVFTKSYVWFQSFNIHYDIGVDGIAVAMIMLTSIVVFAGVFS
                     SWMTKELPREFFISLILLSSGVFGFFISLDMFTMFLFYELAVIPMYLLIGLWGSGNKE
                     YSAMKLTLMLMGGSALLILGLLGIYFHSAPDGGQLTFNILEISKITIPHDTQMFFFPF
                     LFVGFGVLGALFPFHTWSPDGHASAPTAVSMLHAGVLMKLGGYGCFRVAMFLLPDAAQ
                     EMGWLFLILTTVSVVYGAFGAIWQKDLKYINAYSSVSHCGLVIFALLMMNKTSMDGAI
                     LQMISHGLMTALFFALIGMIYGRTHTRNIFEMGGLMKVIPFISVVYVIAGLASLGLPG
                     LSGFVAEMTIFVGSFQHADTFHRV"
     gene            complement(1270..3195)
                     /gene="nuoL"
                     /locus_tag="P5243_01255"
     CDS             complement(1270..3195)
                     /gene="nuoL"
                     /locus_tag="P5243_01255"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRS18101.1"
                     /translation="MDFTYTLAIIGLPFITFLILGLLHNKLSHKLAGIIGSSSLGISF
                     ILSCITAYFYFFKYGKIDGAYQKIEAFNITWLQLTDQLHIDMGILLDPISVMMLMVVT
                     TVSLMVHIYSTGYMHGETGFNRFFAFLSLFSFSMLGLVVATNLFQMYIFWELVGVSSF
                     LLIGYYYEKPSAVAASKKAFITTRFADLGFLIGILLLSFYTGTFDFFELTKTNGHELA
                     MTMPAGTSFLGLSIVSWALILIFMGGAGKSAMFPLHIWLPDAMEGPTPVSALIHAATM
                     VVAGVYLVARLFPVYVLEAPDALTVVMWVGAFSALFAAIIACTQFDIKRVLAFSTMSQ
                     IGYMMLALGVSHYGDHEGLGFMASMFHLTTHAMFKALLFLGAGSIIHAVHSNSMLDMG
                     GLKKYMPITFITFGIAWLAICGFPGLSGFFSKDEILAAAYHHNKVVFYIAVLVAGLTA
                     FYMSRLFFSIFFGKTQHYHHTPHESPASMTIPLIILAIFATFAGYIPFHDLITSDGRP
                     FEAHIDWAIAIPVTLLAFGGMALAFAFYFKPSTMPDKIAASLKGVYTTIYRKFYIDEV
                     YLFVTQKILFNIVSQSIAWFDRHIIDATMDGFATVTNYASESIKELQSGQIQKYGFVF
                     ISGVFVFAFILIYVFVY"
     gene            complement(3217..3534)
                     /gene="nuoK"
                     /locus_tag="P5243_01260"
     CDS             complement(3217..3534)
                     /gene="nuoK"
                     /locus_tag="P5243_01260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRS18102.1"
                     /translation="MIEGIDITHILIVSTCMFFIGVYGFLVRRNLITILMATELILNA
                     VSINFVVLNKFIYPDVLQGYMFSVFIIAIAAAEAAVAIAIIINVYRRYRTINVENVHD
                     MKY"
     gene            complement(3539..4045)
                     /locus_tag="P5243_01265"
     CDS             complement(3539..4045)
                     /locus_tag="P5243_01265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRS18103.1"
                     /translation="MNVIFFYIIAAAILGFSILSVTSRKILRAATFLLFVLISTAALY
                     FLLQVHFLAAVQLMVYAGGIAVLIIFSILLTSNVDFTFEKIDWKKQVFSGLLALGGAA
                     ITILTLLQFNFNSLVTKQTVTPDIRSFGRAFLSYGDGGFVLPFEVITVLLLAAMVAAI
                     MVAKKSKQ"
     gene            complement(4049..4528)
                     /locus_tag="P5243_01270"
     CDS             complement(4049..4528)
                     /locus_tag="P5243_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS18104.1"
                     /translation="MKVVVEYFKDIFGGIKTLITGMKVTGKYFVSPSEIVTQQYPENR
                     ETLQMFDRFKGEVIMPHNDKNEHKCTGCGICEMNCPNGSIEVISKWEIVDDKKKKAID
                     KHIYHLSMCTFCGLCIKTCPSKALAFGQAFEHSVFDRSQLTKILNKPNSVVTKDLKE"
     gene            complement(4539..>5389)
                     /locus_tag="P5243_01275"
     CDS             complement(4539..>5389)
                     /locus_tag="P5243_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRS18105.1"
                     /translation="ADFIKLLMKELIYIKSADQFLFNVAPFIVITASFLAIGAIPFDK
                     GLQVLDFDIGVFYVAAVSSLGVLGVLLAGWASNNKYSLIGAMRSGAQIVSYELSVGLS
                     LMTIIVLAGTMQFSELVAGQADLWFIFKGHIPAMIAFVIFLIAGTAETNRGPFDLAEA
                     ESELTAGFHTEYSGIKFALFFLAEYINMFIVASIATTVFLGGWMPLHIPGLDGFNHVM
                     DFIPPVLWFFMKAFTVVFIMMWFKWTFPRLRIDQLLTLEWKYLLPLNLINIVIMTIVV
                     LLGWHL"
BASE COUNT         1922 a         1068 c          840 g         1559 t
ORIGIN      
        1 tactctatgg aacgtatctg catgttggaa cgaaccaaca aatatggtca tttctgcaac
       61 gaatccactt aatccgggta aacctaatga tgctaaacct gcgataacat atactaccga
      121 aataaatgga attactttca ttaatccacc catttcgaat atgttacggg tatgagttct
      181 tccgtatatc atacctatta aagcaaagaa taaggctgtc atcaaaccat gcgaaatcat
      241 ttgaagtatt gcaccgtcca ttgaagtttt gttcatcata agtaaagcga atatcactaa
      301 tccacaatgc gataccgaag aatatgcgtt gatgtatttt aaatcttttt gccaaattgc
      361 accaaatgct ccatacacta cacttaccgt tgtgagaatc aaaaacagcc aacccatttc
      421 ttgtgcggca tcgggtaata aaaacattgc aactctaaaa catccgtatc ctcccaattt
      481 catcaataca ccggcatgga gcatagatac tgctgtaggt gccgaagcat gaccatcggg
      541 cgaccatgtg tgaaacggaa ataacgctcc taacactcca aatccaacaa aaaggaatgg
      601 gaaaaagaac atttgtgtat cgtgaggaat tgtaattttt gatatttcta gaatattaaa
      661 tgttaattgt cctccatcgg gtgccgagtg aaaatatatg cctaacaatc ccaaaatcag
      721 taaggcagaa ccacccataa gcataagggt aagtttcatt gccgaatatt ctttgttacc
      781 gcttccccac aaacctatca acaaatacat tggaattacc gcaagttcat aaaataagaa
      841 catggtaaac atgtcgagcg aaataaaaaa tccaaatacc cccgaagata atagtataag
      901 tgaaataaaa aattcacgtg gtaattcttt ggtcatccac gaagaaaaaa cacctgcaaa
      961 tactactatc gatgtaagca ttatcattgc taccgcaata ccgtcaacac ctatatcgta
     1021 gtgaatattg aaactctgaa accacacata cgatttggta aataccattt ctgcagtatt
     1081 gcctgctttg cgttcggcaa tatattgaaa caataaatta aacgacatga gcaattgaat
     1141 acccattcca atagccgata ccatgcgagc acctttcaaa tccttagtaa aaaggattcc
     1201 tatcatcgta agaattggta ctacaacaaa taaacttaat atatccataa ttgtaaatct
     1261 aatttctaat taatacacaa atacataaat aagaataaat gcaaacacaa atactcccga
     1321 aataaataca aatccatatt tttgaatttg tcccgattgc aattctttaa tcgattctga
     1381 ggcataattg gtaaccgttg caaatccgtc catggttgca tctatgatat gtctgtcgaa
     1441 ccatgctatg ctttgcgaaa ctatattgaa caatattttt tgtgttacaa ataaatatac
     1501 ttcgtctata taaaatttac gataaatcgt agtatacacg cctttgagtg atgctgcaat
     1561 tttatcgggc atagtacttg gtttgaaata gaatgcaaat gccaatgcca taccaccaaa
     1621 agcaagcaat gttacaggaa ttgcaattgc ccaatctata tgtgcttcaa acggacggcc
     1681 atctgatgta attaaatcgt ggaatggaat gtatccggca aatgttgcaa aaattgctaa
     1741 aataatgaga ggaattgtca tgcttgcagg tgattcatgt ggcgtatgat gatagtgttg
     1801 agtttttccg aagaagatac tgaaaaacaa acgcgacata taaaatgcgg ttaaacctgc
     1861 caccaacaca gcaatataaa atactacttt attatggtga tatgctgccg ctaaaatttc
     1921 gtctttacta aaaaatcctg aaagtccggg gaaaccacat atagctaacc atgcaatacc
     1981 aaaggtaata aacgtaatag gcatatattt tttgagtcct cccatatcga gcatgctatt
     2041 gctatgcacg gcatgaataa tagaccctgc tcctaagaac aacaatgctt taaacatagc
     2101 atgtgtagtt aaatgaaaca tagatgccat aaatcctaag ccttcgtgat cgccatagtg
     2161 cgaaacgcca agcgccaaca tcatatagcc aatttgtgac atggtagaaa aagcaagtac
     2221 tcgtttaata tcgaattggg tacaggctat aattgcagca aataatgcag aaaatgcacc
     2281 tacccacatt actaccgtta aagcatcggg ggcttctaat acatataccg ggaataagcg
     2341 ggcaactaaa tacacacctg ctaccaccat agttgcagca tgtatcaatg ccgataccgg
     2401 cgtaggacct tccatagcat cgggtaacca aatatgaagc gggaacatag cagatttacc
     2461 tgcaccaccc ataaatataa gaatcaacgc ccaacttaca atagataacc ctaaaaatga
     2521 agttccggca ggcatagtca tagctaattc atgtccgttg gttttagtta attcaaaaaa
     2581 gtcgaacgtg ccggtgtaaa acgacaataa taatatacca attaaaaaac ctaaatcggc
     2641 aaatcgtgtt gttataaatg cttttttaga tgctgctact gccgatggtt tttcgtaata
     2701 gtaaccaatc aacaagaatg atgatacacc cacaagttcc caaaatatat acatttgaaa
     2761 caagttggtt gctaccacta agcctaacat tgaaaatgaa aacaacgata aaaatgcaaa
     2821 aaatctatta aatcctgttt caccatgcat ataaccggta ctgtatatat gtaccataag
     2881 cgatacggtg gtaaccacca tgagcatcat taccgaaatt gggtcgagca aaattcccat
     2941 gtcaatatgt aattgatcgg ttagctgcaa ccacgtaatg ttaaacgctt caattttttg
     3001 atatgcacca tcaattttac catatttaaa aaaatagaaa tatgcagtaa tgcacgataa
     3061 gataaatgaa atacctaaag atgaactacc tatgattcct gcaagtttat gacttaattt
     3121 attatgcaat aaacctaata taagaaatgt aataaagggt aatccaataa ttgccagtgt
     3181 atatgtaaaa tccatattgt ttaaattttc tatgtattaa tacttcatat cgtgaacatt
     3241 ctcaacatta atggttctgt atcggcgata cacattaatg ataattgcta tggctactgc
     3301 agcttcggct gcagcaattg caattataaa tacgctaaac atatagcctt gcaaaacatc
     3361 gggatatata aatttgttta acacaacaaa atttatactt accgcattca aaataagttc
     3421 ggtagccatt aaaattgtaa ttaaatttct acgaaccaaa aatccataaa cacctataaa
     3481 aaacatacag gtacttacga ttaaaatgtg tgtaatatca atgccttcta tcatatctct
     3541 attgtttaga tttttttgca accataattg ctgctaccat ggcagccaat aataaaacag
     3601 ttattacttc aaacggtaat acaaacccac cgtcgccata acttaaaaat gctcgtccaa
     3661 acgaacgaat atcgggagta acggtttgtt ttgtaaccag cgaattaaag ttaaattgta
     3721 ataatgtaag aattgttata gcagctccac ccagcgcaag caacccggaa aatacttgct
     3781 ttttccaatc tattttttca aatgtaaaat ccacattact tgtcaacaaa attgagaaaa
     3841 taatcaatac cgctatacct ccggcatata ccataagttg aaccgctgca agaaaatgta
     3901 cttgtagtaa aaaatataat gctgcggtag aaataagtac aaacaataaa aatgtagctg
     3961 cacgcagtat ttttctgctt gtaacgctca aaatagagaa tccgagtata gctgcagcta
     4021 tgatgtaaaa aaagataaca ttcataattt attctttaag gtctttagta actacagaat
     4081 ttggtttgtt taatattttg gtcaactgcg aacggtcaaa taccgaatgt tcgaatgcct
     4141 gaccaaacgc taatgctttt gatggacagg ttttaataca taaaccacaa aaagtacaca
     4201 tactcaagtg atatatatgt ttatcaattg cctttttctt tttatcatct acaatttccc
     4261 atttcgaaat aacttcgata gaaccattag ggcaattcat ttcgcaaata ccacaaccgg
     4321 tacatttatg ctcattttta tcattatgcg gcatgataac ctcacctttg aacctatcaa
     4381 acatttgcaa tgtttcgcga ttttcgggat attgttgtgt tacaatttca ctaggactta
     4441 caaagtattt tccggtaact ttcataccgg taatcaatgt tttgattccc ccaaaaatat
     4501 ctttaaaata ctctactact actttcatat attgcttgtt ataaatgcca gcctaataaa
     4561 actacaattg tcattattac tatgtttatt aaattgagcg gtaataaata tttccattcc
     4621 aatgttaata attggtcgat tcgtaatcga ggaaacgtcc acttaaacca catcataata
     4681 aatactaccg taaatgcttt cataaagaac cataataccg gtggaataaa atccataaca
     4741 tggttaaatc catctaatcc gggtatgtgc aatggcatcc aaccacctaa aaatacagta
     4801 gttgctatag atgctacaat aaacatattg atgtattctg ccaagaaaaa caatgcgaat
     4861 ttaatacccg aatattcggt gtggaatccg gcagtaagtt ccgattctgc ttcggctaag
     4921 tcaaacggac cacgattggt ttcggcagta cctgctatca aaaatattac aaatgctatc
     4981 attgcaggta tgtgtccttt gaatataaac cataaatctg cttgtcctgc tactaattcc
     5041 gaaaattgca tggtacctgc caatactatg atagtcataa gcgacaagcc taccgaaagt
     5101 tcgtaactca ctatttgagc accacttcgc atagcaccta taagtgaata tttgttgttg
     5161 cttgcccaac ctgccaataa tactccaagt actccaagac tcgaaaccgc agctacgtaa
     5221 aatacaccta tatcaaagtc taatacctgt aatcctttgt cgaaaggtat agctcctatt
     5281 gctaaaaatg acgcggtaat tacaataaac ggtgctacat taaataaaaa ctgatcggca
     5341 ctttttatat aaattaactc tttcatcaac aatttgatga aatcggcaa
//
LOCUS       DAOTOW010000044         5374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12812_length_5374_cov_3.477533, whole genome shotgun sequence.
ACCESSION   DAOTOW010000044 DAOTOW010000000
VERSION     DAOTOW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12812_length_5374_cov_3.477533"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="P5243_01280"
     CDS             <1..902
                     /locus_tag="P5243_01280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS18106.1"
                     /translation="DISIPVSAKGEFSYTFTNTYTNYAKIFFNPNDFILLILSPKEKI
                     TITANYNNMSGDNTVKGSKHTELWNSTNANLQQFAKNTQELQKKYERAIDSVEKKRID
                     YVISFVKQNPTSLASLAVIEVLDISMYSNIYEFLDSSLMSVHKGNPIVENFHTNLKQA
                     LFLKEGSLAPDIILQDENGKQIALSSLKGKVVLIDFWASWCRPCRQEIPNLKKAYSIY
                     GNRGFEIYSVSIDNDKNAWFQALSQENMPWITVYDAQKVYSSMYNVTSIPHTLLLGPD
                     GKIIAKNVRGGALEEYLQQIIGK"
     gene            complement(908..1882)
                     /locus_tag="P5243_01285"
     CDS             complement(908..1882)
                     /locus_tag="P5243_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRS18107.1"
                     /translation="MKEFSIPSCIQSDIITFEEVFKKEIATSTRLLNLITSYILKTKG
                     KQLRPILVFLSARMCGSINESTYSAAYMIELLHTATLLHDDVVDNAEKRRNFFSINAL
                     WKNKIAVLVGDYFLAKGLLHAIDKQEFEILHIISNAVKEMSEGELLQVEKARGLNNTE
                     EIYFEIIRKKTAALFIAAMTSGAVSSKQCSQQQKDSIQKIAEILGLAFQIKDDILDYT
                     STNIIGKPAWNDLKEQKMTLPLLHALHKVPKNEQRHIKSLLKKHKNKHSKLQEIVDFV
                     TNNGGIEYAQQKIDELCNEAKQHVNVFPPSESRDAMINLIQFVSTREK"
     gene            complement(1908..2285)
                     /locus_tag="P5243_01290"
     CDS             complement(1908..2285)
                     /locus_tag="P5243_01290"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRS18108.1"
                     /translation="MEALFIKATDETPEITLNHVTGEYCISHRSLPENAIGFYAPVFD
                     WIEKFSQNPPVDEISFIFNLEYFNTASAKQIAKVLLMLEKLSEKIDVTVVWKYKKDDV
                     DMMSSGLRFSKLLNMEFEIQEVE"
     gene            complement(2288..3409)
                     /locus_tag="P5243_01295"
     CDS             complement(2288..3409)
                     /locus_tag="P5243_01295"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HRS18109.1"
                     /translation="MHLEEKSLEQLTNGFKLFEGMQKDNLGYIYRGLFTQNITDSILA
                     LTENNLESVGESSKVKKRVFSIMVECLQNVTRHQTPESVEVVEPEQSGIFVIQTKNNA
                     YQITSGNVVEKKAIPHLKQLLEKINGLEKDALKEYYKQILEDGSISDKGGAGLGLIEM
                     ARKSGNKLYYDFKDINKEISYFYLHTAISKELEEQIESVDLNNIKNLHDIVNKENIML
                     IFNGCLNQDSLINLLSIIEGQSIGAVDIKKKLFNIMVEMLQNIVKHGAKKDENMVGNP
                     AIFFISQVGNNYILNTGNYIESHIVDTLSEKLDHVNSLEGDELDNFYTRRLLNFDIDN
                     SKEAGLGIIDLRIKSGNKILYNFETVNEKISFISMQVKI"
     gene            3611..5002
                     /locus_tag="P5243_01300"
     CDS             3611..5002
                     /locus_tag="P5243_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18110.1"
                     /translation="MRNILILFVLLFFISCVKEDFLSSSQYQLQFSTDTIFFDTIFTK
                     KGSITRFCKLYNPHKGTIVVDEICVGNQQNVQFFINVNGNSASQIKNLRIDGGDSVFV
                     FVQAKLQENNVDTAISHTDSIVLKYNGIRQKIVLQAWGQNVQNIKGVLSQNTTFTSQI
                     PYVVYDSLVVPYGVTLSIEAGAKLYMHYKANIIVHGTLQIHGTKDKPVLITNDRLEQT
                     YQQLPGQWGSIVFSPTSSNNVISFAKIKNGTNGLKITGSSQALVDVSVQACDIHTMSS
                     HIIYAQYAQLNIGNSVLYNSNYYVLAIQGGDCTITHTTISNYGTIGFRNTTSSVIVCN
                     YNLENQNEMILNKFQCLNSIIVGQTTNEISILSLDKNSQLPCAFKYSLIKDTYSGKDT
                     VYYSNIVPFDVNKKLFKSLSMSDFMLDTLSQAKDIGFLPYSQMYETDKAGKSRIIDNK
                     PDAGAYEYFYETK"
     gene            4989..>5374
                     /locus_tag="P5243_01305"
     CDS             4989..>5374
                     /locus_tag="P5243_01305"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRS18111.1"
                     /translation="MKLNNIVVCLVLVFSISSVFSQESQKLVFSAMFYNVENLFDTTD
                     NPMTQDNDFLPESKKQWNTPKYYVKLRNISRVIVAAGKWHVPDIIGLCEVETADCLWD
                     LTHKTNLSRLEYSYIHYESLDNRGIDV"
BASE COUNT         1774 a          770 c          838 g         1992 t
ORIGIN      
        1 tagatatatc aattccggta tcggcaaaag gcgaattctc ctatactttt actaatacat
       61 atacaaatta tgcaaagata ttttttaatc ctaatgattt tattttgtta atactttcac
      121 caaaagaaaa aataactatt acagccaatt ataataatat gtcaggcgac aatacggtta
      181 aaggttctaa gcatactgaa ttgtggaata gtacaaatgc aaatttgcag caatttgcaa
      241 aaaatactca agaactgcaa aaaaagtatg aacgagctat agattctgtt gagaaaaaac
      301 gtattgacta tgtaatttca tttgttaaac agaatcctac atcattagct tcgcttgctg
      361 taattgaagt tttagatata agtatgtatt ccaatattta tgaatttctt gattcaagtt
      421 taatgtcggt acataaaggt aatccaattg tagaaaattt tcatactaac ttaaagcaag
      481 cactgtttct taaggaaggt tccttggctc ctgatataat attgcaagat gaaaatggta
      541 aacaaatagc tttgtcatct ctcaaaggaa aagtagtttt aattgatttt tgggcaagtt
      601 ggtgtaggcc atgtcgtcaa gaaataccaa atctaaaaaa ggcttatagt atatatggaa
      661 atcgcggatt tgaaatttac tccgtttcaa tagataatga taaaaatgcc tggtttcaag
      721 ccttaagtca agaaaatatg ccttggatta cggtatatga tgctcaaaaa gtatatagca
      781 gtatgtacaa tgtaacttct atacctcata cgttgttatt aggacccgat ggaaaaataa
      841 ttgcgaaaaa tgttcgtggt ggtgctttag aggaatattt acagcaaata atcggaaaat
      901 agaagtatta cttttctcgt gttgatacaa attgaatgag attaatcata gcgtcgcgcg
      961 attcacttgg aggaaataca tttacatgtt gtttagcttc gttgcataat tcatcaattt
     1021 tttgttgagc atattcaata cctccattat ttgttacaaa gtcaacaatt tcttgtagtt
     1081 tactgtgttt gtttttgtgt tttttaagta atgattttat gtgtctttgt tcattctttg
     1141 gtaccttatg taaagcatgt aaaagaggaa gtgtcatttt ttgttctttt aaatcattcc
     1201 atgcgggttt accaattatg tttgttgaag tatagtctaa aatatcatct tttatttgaa
     1261 atgctaagcc taaaatttca gcaatttttt ggatggaatc tttttgttgt tgtgaacatt
     1321 gtttgcttga tacagctcct gaagtcatag cggcaataaa tagagcggct gttttcttac
     1381 gaattatttc aaaatatatt tcttcggtat tgtttaaacc acgagctttt tcaacttgca
     1441 agagttcgcc ttcgctcatt tctttaacag catttgaaat tatgtgtaaa atttcaaatt
     1501 cttgtttatc tatagcatga agtagtcctt tggctaaaaa ataatctcca actaaaacag
     1561 caattttgtt tttccataaa gcattgattg aaaaaaaatt acgacgtttt tcggcattgt
     1621 ctacaacatc gtcatggagc aaggttgctg tgtgtaaaag ttcaatcata tatgctgcag
     1681 aataggttga ttcgtttata gaaccacaca tgcgtgctga tagaaaaacc agtatagggc
     1741 gtagttgttt acctttggtt tttaaaatgt atgaagttat aagattgagt aatctggtag
     1801 atgtagctat ttctttttta aagacctctt caaatgttat tatgtctgat tgaatacatg
     1861 aaggtataga aaattctttc atagatagaa taggatatgt aaataaatta ttctacttct
     1921 tgaatttcaa attccatatt caacagtttt gagaatcgta atcccgacga catcatatct
     1981 acgtcgtctt ttttatattt ccaaactacg gttacatcta ttttttcaga taatttctct
     2041 aacataagta atacttttgc aatttgtttt gccgaagctg tattgaaata ttctaaatta
     2101 aaaataaatg atatttcatc taccggaggg ttttgtgaaa atttttcaat ccaatcaaat
     2161 acaggggcat agaagcctat tgcattttca ggcaacgaac ggtgtgaaat acaatattct
     2221 cctgtaacat ggttcaatgt aatttcagga gtttcatcgg ttgcttttat aaagagtgct
     2281 tccatattta tatttttacc tgcattgata taaacgaaat tttttcgttt acagtttcaa
     2341 aattatataa aattttgttt ccgcttttaa ttcgtaagtc aattattcct aatccggctt
     2401 ctttactatt atctatatca aaatttaaaa gtctgcgagt gtagaaatta tctaattcat
     2461 caccttctaa actattaaca tgatctaatt tttctgatag ggtatcaaca atatgtgatt
     2521 caatatagtt tcctgtattg agaatgtaat tgttgcctac ttgcgatata aaaaatatag
     2581 caggatttcc aaccatattt tcatcttttt tggctccatg ttttacaata ttttgaagca
     2641 tttcaaccat tatgttgaaa agtttctttt taatatctac agcgcctatg ctttgtcctt
     2701 caattataga caataaattt attaaactat cttgattgag gcagccatta aaaattaaca
     2761 tgatgttttc tttattaaca atatcatgta aattttttat gttatttaaa tctaccgatt
     2821 ctatttgttc ttcgagttct tttgaaatag ctgtgtgaag atagaaatat gagatttctt
     2881 tgtttatatc tttaaaatcg taatataatt tgttgcctga tttacgagcc atttcaatta
     2941 atcctaatcc tgctccacct ttatcagaaa tactgccgtc ttctaatatt tgtttgtagt
     3001 attcttttaa tgcatctttt tctaaaccat taattttttc aagtagttgt tttaaatgag
     3061 gaatagcttt tttttctaca acattaccag atgtaatttg gtatgcatta ttttttgttt
     3121 gtattacaaa aattcctgat tgctcgggtt caacaacttc aacactttca ggcgtttggt
     3181 gtcgagtaac gttttgcaag cactcaacca taattgagaa aactcttttt tttactttag
     3241 atgactcacc aaccgactct aaattatttt cggttaaagc caatatgctg tcggtaatat
     3301 tttgagtgaa taaaccacgg tatatatacc ctaggttatc tttctgcata ccttcaaata
     3361 gcttaaaccc attggttaat tgctcaagac ttttttcctc cagatgcatt ctaaacagtt
     3421 ttaattatgg tactatcaac taacaaatat agttatttat acttaaaaac caaaaaaaag
     3481 ttatttaact cttctctatt cttgttacat ttgtaaaaaa aaaacgattt ttttacaaaa
     3541 aagtttagtt tacaaaaata gaatatattt tttaaataat cgttttgttt gttgaatttt
     3601 actaatgcgt atgcgtaaca tcttaatttt atttgtttta ctatttttta ttagttgtgt
     3661 caaagaagat ttcctatctt catctcaata tcaattacaa ttttcaaccg atactatttt
     3721 ttttgataca atttttacaa aaaaaggtag tataacaaga ttttgtaaat tgtacaatcc
     3781 tcataaagga actatagtag tagacgagat ttgtgtagga aaccaacaaa acgttcaatt
     3841 ttttataaat gttaacggaa attctgcttc gcaaattaaa aatcttagaa ttgatggtgg
     3901 tgatagtgtt tttgtttttg tacaagctaa attgcaagaa aataatgtag atacggcaat
     3961 ttctcatact gattcaattg ttttgaagta taatggaatt cgccaaaaga ttgtattgca
     4021 agcctggggg caaaatgttc aaaacataaa gggtgtactt tctcaaaata ctacatttac
     4081 ctcgcaaata ccatatgttg tttatgattc gttggttgtg ccttatggag taactctttc
     4141 tatagaggcg ggggcaaaat tgtatatgca ttataaggct aatataatag tgcatgggac
     4201 actgcaaata catggtacaa aagataaacc ggtacttatt acaaatgaca gattggaaca
     4261 aacctatcaa caattgccgg gacaatgggg aagtatagtg ttttctccta caagttctaa
     4321 caatgtaatt tcatttgcta aaattaaaaa tggtactaat ggattaaaaa ttacaggttc
     4381 ttctcaggca ttggttgatg tgtcagtgca agcatgtgat atccatacta tgagttctca
     4441 tattatatat gcacaatatg cacaattgaa tataggtaat tcggtgctgt ataatagtaa
     4501 ttattatgtt ctggcaattc aaggaggtga ctgtacaatt actcatacta ctatttcaaa
     4561 ttatggaact attggattta gaaatacaac ttcatctgtc atagtgtgta attataattt
     4621 agaaaatcaa aatgaaatga tattgaataa gtttcaatgt ttaaattcaa taattgttgg
     4681 gcaaactact aatgaaattt caatactttc gctcgataaa aattctcaat taccctgtgc
     4741 atttaagtat agtttgatta aagatactta tagtggaaaa gatactgtgt attatagcaa
     4801 tattgtacca tttgatgtaa ataaaaaatt atttaaaagt ttatcaatga gtgattttat
     4861 gctcgataca ctttcacaag ctaaagacat aggttttttg ccatattcgc aaatgtatga
     4921 aaccgataaa gccggaaaat ctcgtataat tgataataaa cctgatgccg gagcatatga
     4981 atatttttat gaaactaaat aatatagtag tttgtttggt tttggttttt tcaatttctt
     5041 cggtattttc ccaagaatct caaaaacttg ttttttcggc aatgttttat aatgtagaaa
     5101 atctgtttga tactactgat aatcctatga cacaagataa tgattttctg cctgaatcta
     5161 aaaaacaatg gaatacccca aagtattatg taaagttacg aaatatatct agggttatag
     5221 tagctgccgg taaatggcat gtacccgata ttattggatt gtgtgaagtt gaaaccgctg
     5281 attgtttgtg ggatttaact cataaaacaa atctttcacg attggaatat tcctatattc
     5341 attatgaatc gctcgataat agaggaatag atgt
//
LOCUS       DAOTOW010000045         5338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_12913_length_5338_cov_2.838349, whole genome shotgun sequence.
ACCESSION   DAOTOW010000045 DAOTOW010000000
VERSION     DAOTOW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12913_length_5338_cov_2.838349"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="P5243_01310"
     CDS             complement(<1..1448)
                     /locus_tag="P5243_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HRS18112.1"
                     /translation="MKQIYKEILIVIFAIFTQISFTYSQVSEAPIGNGTSENPYQIST
                     LENLYWISQNPASWDKYFIQMHDINAAATATWDSNKGWTPIGNNVQKFNGFYNGNNYI
                     ISNLYIDRPTENNVGLFGHVGHVNSDTEPTLIKSLGIVNASVNGARGVGALIGRVTGN
                     QNTIIEYCYAIGGTIKGDGATGGLIGSLNSFEETSDGSKNPVLRKSYSTNSVQSSGNG
                     TLDKFGGLVGCSQKGSIQNSFARGHVTVNNGTRVGGLVGCIDYRGDVENSYATGEVTT
                     TASTNVGGLVGNISGIGTYDGEVTNSVWDTEVSTLGSSAGGTAQNTSAMKTSATYSSL
                     SWDITTTWGIAAGINNGYPYLLGDNNFILPIELASFEGKRISSEIIELRWTTESEFNN
                     EGFEIQKSYNGTTFHTIAWIKGKGNSSIQQEYSFLDYESSTNIVYYRLVQFDRNGDYT
                     YSDIITINSVTPPKEDLLIINKQCVLQNAEQDA"
     gene            1652..3301
                     /locus_tag="P5243_01315"
     CDS             1652..3301
                     /locus_tag="P5243_01315"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRS18113.1"
                     /translation="MAISALQKERAAYAPKLPKSLQGYVTLVDGAATQSVADQAEIKA
                     LFPNTYGMPLITFAEGSKKEYPAINVGVILSGGQAPGGHNVISGLFDGLKKLNPANKL
                     YGFLGGPGGLVDHKYIELTSSIIDEYRNTGGFDIIGSGRTKLEETAQFDKGVEICNKL
                     GIKALVIIGGDDSNTNACVLAEYYIQKNTGIQVIGCPKTIDGDLKNEMIETSFGFDTA
                     CKVYSELIGNIQRDANSAKKYWHFIKLMGRSASHIGLECALQTQANICIISEEVEAKQ
                     QTLDQVVTYIADIVAKRAANGENFGTALIPEGLIEFIPSLKVLISELNDTMAHNEAAF
                     NALASDAEKFQFIMGKLSKTSADIFATLPQNIQFQLLADRDPHGNVQVSKIETEKMLI
                     DMVDAKLKAWKKEGKYVGKFSGQNHFFGYEGRCAAPSNFDADYCYSLGVTASVLIGEG
                     KTGYMASVRNCKAPASEWIAGGVPVTMMMNMEKRHGHMKPVIQKALVTLDGKPFKFFA
                     SKRDEWAIKTDYIYPGPIQYFGPSSVCDQPTKTLLLEQEGK"
     gene            complement(3354..4871)
                     /locus_tag="P5243_01320"
     CDS             complement(3354..4871)
                     /locus_tag="P5243_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRS18114.1"
                     /translation="MRISELDEHISSILKDIVQKEKNIELLISGISESYKLSAKNLCR
                     YLILRNYDLTKYHDSLSELGLSSLRTAEGYVYSNLFSIVKNLKLIQGLPFKKKKEIEI
                     VGYKRSKQLIKTHANELFNKKQKKQFTEIMVTLPNEAAKDKSIIKDMVKNGMEIARIN
                     LSHGNIEMWTTMVQYIHEVMAETKKEVKIYMDLSGPKIRTSQIEIRGKKGKVKNALVI
                     RQGERIIVTKRTTLGKKSKFDKKNGQLQKAEIGVMLHEIIDDVQVNDVILFDDGMIKA
                     IVVAKYTDEVEIEITDCYKTKLSSHKGINLPNTKLHLPALTEKDIEYLPFVCKHANIV
                     GYSFVRSAHDVACLYKELETYNAHDLGVVFKIENKEAFENLPFILLEGMKRPKIGVMI
                     ARGDLAVEIGFENISEVQNQILLLCEAAHVPVIWATQVLENLAKTGIPTRAEISDVVL
                     GVQAECIMLNKGKYILEAIKTLKNILIKIEPNSFKNKHSLKPLEIAIHTCNTLFA"
     gene            5045..>5338
                     /locus_tag="P5243_01325"
     CDS             5045..>5338
                     /locus_tag="P5243_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRS18115.1"
                     /translation="MAKVLIIGAGGVGRVVAHKCAQVPEVFEEIMLASRTKSKCDAIA
                     KSIPNRTIQTAQVDAENVPELVALIRSFKPDMVINVALPYQDLFIMDACLETGV"
BASE COUNT         1773 a          904 c          769 g         1892 t
ORIGIN      
        1 gcatcttgtt ctgcattttg taatacacat tgcttattta ttataagtaa atcttctttt
       61 gggggagtta cagaattgat tgttataata tctgaatatg tataatcacc atttctatca
      121 aattgaacaa gtctataata cacaatattt gtagaacttt cataatctaa aaagctatat
      181 tcttgttgta ttgaactatt acctttacct ttaatccatg caatagtatg aaacgtggtt
      241 ccattataac ttttttgtat ttcaaaacct tcattattaa attcagattc tgtagtccaa
      301 cgtaattcaa taatttctga agatattctt ttaccttcaa aacttgctaa ttcaattggt
      361 aaaataaaat tattatcacc taacaaatat ggatatccat tatttatacc tgctgcaatt
      421 ccccacgttg tagtaatatc ccatgataaa cttgaatatg ttgccgaagt tttcattgca
      481 ctggtatttt gagcagttcc acctgccgat gaccccaatg tacttacttc tgtatcccac
      541 acagaattag taacttctcc atcataagtc ccaattcctg aaatattacc taccaatcca
      601 ccaacatttg ttgatgctgt tgttgtaact tcacccgtag catacgaatt ttctacatct
      661 cctctataat caatacagcc cactaatcca cctacacgtg ttccattatt aaccgttaca
      721 tgacctcgag caaaagaatt ttgaatacta cctttttgag aacatccaac taaaccgcca
      781 aatttatcga gtgttccatt tccacttgat tgcaccgagt ttgttgaata acttttacgt
      841 aacaccggat ttttagaacc atcagaagtt tcttcaaaag aatttaaaga accaattaaa
      901 cctcctgtcg cgccatcacc ttttatcgta cctcctattg cataacaata ttctataatt
      961 gtattttgat ttcctgttac acgtcctata agagctccta ctccacgtgc tccattaact
     1021 gaagcattta ctatacccaa actttttatt aatgtaggtt cagtatcaga atttacatgt
     1081 cctacatgcc caaataaacc aacattattt tctgtaggtc tgtcaatata taaattgctg
     1141 attatataat tatttccatt ataaaaacca ttaaactttt gaacattatt tcctataggg
     1201 gtccatcctt tattactatc ccacgttgca gtagctgcag catttatatc gtgcatttga
     1261 ataaaatatt tatcccaact tgccggattt tgagatatcc aatataaatt ttctaatgtt
     1321 gaaatttgat atggattttc agatgtacca ttaccaatag gagcttctga aacttggctg
     1381 taagtaaatg aaatttgagt aaaaattgcg aatattacaa taagaatttc tttgtaaatt
     1441 tgtttcattt tatttaaatt aagttattgt attacaatta tttaattaat attttgcgtt
     1501 tggtttacac tgttttctac gcctcatttt tttaattggt tgcatgattt tttaaatttt
     1561 tagaaaattg cgtatatgta ccaattaaaa attcgtattt tcgcaatcga taaaatttta
     1621 catacaataa ttatataaaa aactatacaa aatggctata agtgcattac aaaaagaacg
     1681 agcagcatat gctccaaaac tgccaaaatc attacaagga tatgtaacct tggttgatgg
     1741 tgcagctaca caatctgttg ccgatcaggc agaaattaaa gcattgtttc ctaataccta
     1801 tggaatgcct ttaataactt ttgcagaagg ttctaaaaaa gaatatccag caataaatgt
     1861 tggtgtaatt ctttcaggag gacaagctcc gggtggacac aatgtgattt cgggtttatt
     1921 tgatggactt aaaaaattaa atcctgcaaa taaattatat ggatttttag gtggccccgg
     1981 tggattagtt gatcataaat atatagaatt aacctcatca attatagatg aatatagaaa
     2041 taccggagga tttgacataa ttggtagcgg tagaacaaaa ttagaagaaa ctgctcaatt
     2101 tgataaagga gttgaaatat gcaacaaact tggaattaaa gcattagtta tcataggtgg
     2161 cgatgattct aataccaatg catgtgtttt agctgaatat tatattcaaa aaaataccgg
     2221 tattcaagtt attggctgcc ccaaaacaat agatggagac ttaaaaaacg aaatgattga
     2281 aacatcattt ggttttgata cagcatgtaa ggtatatagt gaattgattg gtaatattca
     2341 acgcgatgct aattcggcaa aaaaatattg gcattttatt aaattaatgg gacgttcggc
     2401 ttcacatata gggttagaat gtgcattaca aacacaagcc aacatttgca ttatttctga
     2461 agaggtagaa gcaaaacaac aaactcttga tcaggtggta acatatatag ccgacatagt
     2521 tgctaaacga gctgcaaacg gtgaaaactt tggtacagca ttaatacctg agggattaat
     2581 tgaatttata ccttcattaa aggtgttaat ttctgaatta aacgatacta tggcacacaa
     2641 tgaagctgct tttaatgctc ttgcatctga tgctgaaaaa tttcaattta tcatgggtaa
     2701 attatcaaaa acatcggcag atatttttgc aacattacct caaaatattc aattccaatt
     2761 attagcagat cgtgacccac atggtaacgt tcaagtttca aaaattgaaa cagaaaaaat
     2821 gttaatagat atggttgatg caaaattaaa agcatggaaa aaagaaggta aatatgttgg
     2881 taaattcagc ggccaaaatc acttttttgg ttacgaaggt cgttgtgctg ctccttcaaa
     2941 ctttgatgca gactattgtt actcattagg ggttacagca tcggtattaa ttggtgaagg
     3001 taaaaccgga tatatggcat ctgttagaaa ttgtaaagct cccgcatctg aatggattgc
     3061 aggtggtgtt ccggtaacaa tgatgatgaa tatggaaaaa cgtcatggac acatgaaacc
     3121 ggtaatccaa aaagcattag ttacacttga tggaaaacca ttcaaattct ttgcttcaaa
     3181 acgcgatgaa tgggctataa aaactgatta tatttatccg ggaccaattc aatattttgg
     3241 accaagttca gtatgcgatc aaccaaccaa aactttatta cttgaacaag aaggaaaata
     3301 aaaaaaattc aatactacac taaaaaagct acctcaaata ggtagctttt tttttatgca
     3361 aataatgtat tgcatgtatg tattgctatt tctaaaggtt tgagtgaatg tttattttta
     3421 aacgaattgg gttctatttt aatcaaaatg tttttaaggg ttttaattgc ttcaagtata
     3481 tattttcctt tgttaagcat gatacattca gcttgaactc ctaacaccac atcacttatt
     3541 tctgcacgag ttggaatacc tgtttttgct aaattttcta atacttgtgt tgcccaaatt
     3601 acaggaacat gtgccgcttc acacaacaaa agtatttgat tttgaacttc agaaatattt
     3661 tcgaatccta tttctacagc caaatcacca cgagctatca taacacctat tttaggacgc
     3721 ttcatacctt cgagtaatat aaacggtaaa ttttcgaatg cttctttatt ttcaatttta
     3781 aacacaactc ctaaatcatg tgcattatat gtttctaatt ctttatataa acatgctaca
     3841 tcgtgagccg aacgaacaaa agaatatcct acaatattag cgtgcttaca tacaaacggt
     3901 aaatattcta tatctttttc ggttaaagca ggtaaatgca attttgtatt tggtaaattt
     3961 attcctttat gcgatgacaa ttttgtttta taacaatcgg ttatttcaat ttcaacctcg
     4021 tctgtatatt ttgcaactac tattgcctta atcattccat catcaaataa aataacatca
     4081 ttcacttgca catcatctat tatttcatgc aacattacac ctatttcagc cttttgtaat
     4141 tgcccatttt ttttatcaaa ttttgatttt ttcccaagtg ttgttctttt tgttacaata
     4201 attcgttctc cttgacgaat aactaatgca tttttaacct ttcctttttt tccgcgaatt
     4261 tcaatttgtg aggttctaat tttaggaccc gataagtcca tataaatttt aacttctttt
     4321 ttagtttctg ccattacttc atgaatatat tgaaccatag tagtccacat ttcaatattt
     4381 ccatgactta aatttatacg agctatctcc attccgtttt ttaccatatc ttttatgata
     4441 cttttatcct tggcagcttc attgggtaac gtaaccataa tttcggtaaa ctgttttttt
     4501 tgttttttat taaacaattc attggcatga gtttttatta attgcttact ccgtttataa
     4561 ccaactatct caatttcttt tttcttttta aatggtagcc cttgaattaa ttttaaattc
     4621 tttacgatac taaataaatt tgaatacaca tatccttctg ctgtacgcaa cgacgacaat
     4681 cctaattctg aaagagaatc atgatacttt gttaaatcat agttgcgtaa tattaaatat
     4741 cgacataaat tttttgcact caatttatat gattcgctta ttccggatat gagcaattct
     4801 atattctttt ctttttgtac aatatctttc aaaatagaac taatatgttc atctaattcc
     4861 gaaattctca tgtaatttta tttgtgattg aaaacggcca aatataataa aatacttata
     4921 gctcattgtt agataaatat taatttttac ccttatgaaa attcctgctt gagacttttt
     4981 atttttttag acatattata aataaaatta ttaaattagc cacgaaatta aaataaaata
     5041 aactatggca aaagtattaa taataggagc cggaggtgtt ggtcgcgtag tggctcacaa
     5101 atgcgcacaa gtacccgaag tttttgaaga aattatgctg gcaagtcgca caaaatcaaa
     5161 atgtgatgct attgccaaat ctatacctaa cagaactatt caaacagctc aagttgatgc
     5221 agaaaatgta cctgaattag ttgcattaat acgttctttt aaacctgata tggtaataaa
     5281 tgttgcatta ccatatcaag acttatttat aatggatgct tgtttagaaa ccggtgta
//
LOCUS       DAOTOW010000046         5275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13079_length_5275_cov_3.209579, whole genome shotgun sequence.
ACCESSION   DAOTOW010000046 DAOTOW010000000
VERSION     DAOTOW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13079_length_5275_cov_3.209579"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="P5243_01330"
     CDS             <1..241
                     /locus_tag="P5243_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRS18116.1"
                     /translation="AILTEEVGELARIMSRTYGEQSFKPGETASIADELADVFWVLVC
                     IANQTGVNLTEAFTHNIEKKKKRDALRHISNKKLQ"
     gene            536..1036
                     /locus_tag="P5243_01335"
     CDS             536..1036
                     /locus_tag="P5243_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRS18117.1"
                     /translation="MQKILYEKYASKLYALCMRYAKDRAEADDILQEGFIKIYTKIDQ
                     FSQEHSFEGWIKRIIINTAITHYNQNLKHYYQEDIDQINELEITNDEEVYEAEYSQEE
                     LLNIIHSLADGYRMVFNLYVIEGYKHKEISEILGIDIATSKSQLHRAKKIIQEKLEEI
                     AKVKNN"
     gene            1038..2621
                     /locus_tag="P5243_01340"
     CDS             1038..2621
                     /locus_tag="P5243_01340"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18118.1"
                     /translation="MHKSGEHIEDLFGKAFRDAEITPPSHLWESLESSLEKEHAVETL
                     FKKTFVNTAIQPSKKIWYRISYILFLRGFLTFKPTTFNAYYAALTAIVGVVCVSFWPF
                     QKSTTQETPNTNIEIVSNTNQKQIISEQPQETAITKTAPVINQKSTTESEKLMTNNIE
                     QKHTKVEVSAPIPLETFDFSKVRIIGATKICSALTAEYAIEGVPVESEIEWNLQTKDG
                     IIKYISSRKISASWNKGGSYSIWAYVKVGNTKKKIELPVTVEANVEPIIKGKNKVCEG
                     TEKQLYSVDEPVDKNIVYEWKTTYNAIDFIGNKYINVDWKKSGRDTLFVSRINSETGC
                     KSQSAMLVTILPKPIVDFEMTPLGNSEFQFMYTGPIKKGVNIEWSIDGNEFNDQTIVY
                     ENKGIGNTTVTLKVTDKNNCSTILQREVSFNKHIIFVPRQFNLDNNDGFIPQTNQPLK
                     SYKIEIYNPRNEKIWESTDLTNGKPSKAWDGTYKGTQVARGKYMWRIKAVFDDGTEWK
                     GIMQPTGELKPSGIIIIDN"
     gene            2752..3426
                     /locus_tag="P5243_01345"
     CDS             2752..3426
                     /locus_tag="P5243_01345"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRS18119.1"
                     /translation="MNKTSLYVIIVAGGKGLRMQSNIPKQFMLLAGKPILQHSLEAFY
                     NYNQSIICIVVLPKDQIAYWNQLCKDYNCNVPHIIAEGGSERYFSVLSGLQHITTHGF
                     VAIHDGVRPLVSYEVIAQGFAHAFTHSNAIPVVDSVDSLRYVEKDTNYVIHREHIKRV
                     QTPQIFDTKKLLDAYTIGFDTSCTDDATVWEKAGNTVFCYKGDEKNIKITTNTDIHIA
                     ELFMKM"
     gene            3433..4893
                     /locus_tag="P5243_01350"
     CDS             3433..4893
                     /locus_tag="P5243_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS18120.1"
                     /translation="MKEIQKTYEYLHPVTLAFRDATIEKEYHAHYRETYITANRLCIF
                     LAIGLYAIFGILDFFNSSHDSTLYQTIRYGFTIPLSVIIFILSLHSSIATKNHTLFTI
                     GLLFFAISILTINLIAEQNSFSTYNMGFVLLFFFGQNFLKISFFRSTSILLAILLIFE
                     IYTLAFKYVATDVFITISFFLFVSYLLSTFSSYFFELIDRKNYWNSIQLQKTNEELKN
                     LQKLMEQKVTERTNMLEKVVKELQTAKAKAEESNIIKSTFLSTISHEIRTPLTSILGF
                     TQLITKTKTYDEKTQVYAATIEKSIAELLDIMTNILTLSEIHNDRLEKSTTTIHFKTL
                     KKQILAISEEILARRKKNLKFQIISNEPVEIEIPVGEKELLMVIKQLIDNAIKYTGAG
                     HFSIECIINELGMFQICVKDTGIGINAENLHSIFDFFIQEDQLDTRMYNGIGVGLSIC
                     KGIITKAGGKIWAESEKNKGSAFYVTIPIEYNSNNE"
     gene            4907..>5275
                     /locus_tag="P5243_01355"
     CDS             4907..>5275
                     /locus_tag="P5243_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRS18121.1"
                     /translation="MNAVEQKLVEFSEKIIPWLLSHGIKIIAIIVVSYIINKIIYKII
                     DRAVRIAVVGDRYSSREAELKREETLISIFTTTVKIALIVIVSLMVLKEIGLDIAPLL
                     AGAGIVGLALGFGGQYLIRDI"
BASE COUNT         2103 a          750 c          799 g         1623 t
ORIGIN      
        1 agctattctt accgaagagg ttggtgagtt ggcaagaata atgagtagaa cttacggaga
       61 acaatcattt aaacccggag aaacagcatc gattgccgat gaattagcag atgttttttg
      121 ggttttagta tgcatagcaa atcaaaccgg cgtaaactta acagaagcat ttactcacaa
      181 tatagaaaaa aaaaaaaaac gcgatgcttt gcgtcatatc tcaaacaaaa agctacagta
      241 aacatccttt ctacataata ttataacata cataatcaaa aatttcaaat tatgtatttg
      301 ttaataaatt gatgtacacg ccaatacgat tatatttata tttttaatat atttaataca
      361 ataaagtaaa atagagctat taacatacat gttaataact ttgttaatta cttgcaatat
      421 tttttttaaa gaaccatgca accattttag aaaaacatat gtcttataaa taacaaagaa
      481 aataatgttt actgaaaaag aaataattga tggatgtata aagaatgatc ggaaaatgca
      541 gaaaattctc tatgagaaat atgcatcaaa actctacgct ttgtgtatgc gatatgcaaa
      601 agatagagcc gaagccgatg atatattgca agaaggattt attaaaatat atacaaaaat
      661 agaccaattc tcgcaagaac attcttttga aggctggata aaacgaatta taataaatac
      721 agcaatcact cattacaatc aaaacttaaa acattattat caagaagata tagatcaaat
      781 aaatgaatta gaaattacaa atgatgaaga agtatatgaa gctgaatatt cccaagaaga
      841 attgttgaat ataatacata gtttggcaga cggatataga atggtgttta acttgtatgt
      901 aatagaagga tataaacaca aagaaatatc tgaaatttta gggatagata ttgcaacttc
      961 aaaatcacaa cttcatagag caaaaaaaat aattcaagaa aaattagaag aaatagcaaa
     1021 agttaaaaac aattgagatg cataaatcag gggaacatat agaagactta ttcgggaaag
     1081 catttcgtga tgcagagatt actccgccgt ctcatttgtg ggaatcatta gagtcttcgc
     1141 ttgaaaaaga acatgctgtt gaaacattat ttaaaaaaac atttgtaaac acagctatac
     1201 aaccttcaaa aaaaatatgg tatcgaattt catacatatt gtttttacgc ggatttctta
     1261 cttttaaacc aactacattt aacgcttact atgctgcact tactgcaatt gtaggggttg
     1321 tttgtgtatc attttggcca ttccaaaaat caacgacaca agaaactcca aatacaaaca
     1381 ttgaaatagt aagtaatact aatcaaaaac agataatttc agaacaacct caagaaacag
     1441 caattaccaa aactgctcct gttataaatc aaaaatcaac aacagaatcc gaaaaattaa
     1501 tgactaataa tatagagcaa aaacatacca aagtagaagt aagtgctcct atacctttag
     1561 aaacatttga tttttcaaaa gtacgtataa taggtgcaac aaaaatttgt tcggctctta
     1621 ctgcagaata tgctattgaa ggtgtacccg tagaaagtga aattgaatgg aatttacaaa
     1681 ccaaagatgg tattataaaa tatatttcaa gtcgtaaaat ttctgcttcg tggaacaaag
     1741 gcggctcata tagtatatgg gcttatgtaa aagtaggaaa taccaagaaa aaaatagaat
     1801 tacctgtaac tgttgaggca aatgtagaac caatcataaa aggtaaaaac aaagtatgcg
     1861 aaggcacaga aaaacaatta tattctgtag atgaaccggt tgataaaaat attgtatatg
     1921 aatggaaaac aacctataat gctattgatt ttattggaaa taaatatata aacgtagatt
     1981 ggaaaaaaag tggacgcgac actctctttg tatcacgtat aaatagtgaa acagggtgta
     2041 aatcacaatc agcaatgctt gttacaattt taccaaaacc aattgttgat tttgaaatga
     2101 ctccattagg taactcagaa ttccaattta tgtatacagg acctattaaa aaaggtgtta
     2161 atatagaatg gtctatagat ggcaatgaat ttaacgacca aactatagtg tacgaaaata
     2221 aaggtatagg aaatactact gttacattaa aagtaactga caaaaacaat tgtagcacaa
     2281 tacttcaacg cgaagtttca tttaataaac atattatttt tgtaccacgt caattcaatt
     2341 tagacaataa cgatggtttt atacctcaaa caaatcaacc attaaaatca tataaaattg
     2401 aaatttacaa tccccgaaat gaaaaaatat gggaatcaac tgatttaaca aatggtaaac
     2461 catctaaggc gtgggacgga acttacaaag gaactcaggt agctcgtggt aaatatatgt
     2521 ggagaataaa agcagttttt gatgatggaa cagaatggaa aggcataatg caacctaccg
     2581 gtgaattaaa accaagcggc attattataa ttgacaatta atacaacttc ataaatacct
     2641 tactttgaac gccctgtata tcaatatatc agggcgttct tttttcaatt ttcataatac
     2701 cattcaaaat aacaaaagaa tttacgtata tttgtatata atcttgaaaa aatgaataaa
     2761 acttcgttgt atgtaattat agtagcagga ggtaaaggat tgcgaatgca aagcaatatt
     2821 cccaaacaat ttatgctact ggctggaaag ccaattttac aacatagttt agaagctttt
     2881 tataattata atcaatccat tatatgcatt gttgttcttc ctaaagacca aattgcttac
     2941 tggaatcaat tatgtaagga ttataattgc aatgttccac atataatagc agaaggtgga
     3001 agcgaacgat atttttcggt tctttccgga ttacaacata ttaccacaca cggttttgtt
     3061 gcaattcacg atggtgtgag acctcttgtt tcatatgagg taatagctca aggatttgcg
     3121 cacgcattta ctcattccaa cgctattcct gttgttgatt cagttgattc tttgcgatat
     3181 gtagaaaaag ataccaatta tgtaattcat agagaacata taaaacgagt acaaacccct
     3241 caaatatttg acacaaaaaa attattagac gcctatacga taggatttga tacaagttgt
     3301 accgacgatg ccaccgtatg ggaaaaagca gggaatacgg tattttgtta caaaggtgat
     3361 gagaaaaata taaaaattac aaccaacaca gatattcaca ttgcagaatt atttatgaaa
     3421 atgtaaccaa acatgaaaga aattcaaaaa acttatgaat atttacatcc tgttacatta
     3481 gcttttagag atgctactat agaaaaagaa tatcatgccc attatagaga aacctatatc
     3541 acagctaatc gcctttgcat ttttttagca ataggattat atgctatttt tggaatactt
     3601 gactttttta attcatcaca tgacagtact ctatatcaaa ctattcgata tggatttaca
     3661 attccgttat ctgttattat ttttatactc agcctacatt catctattgc aactaaaaat
     3721 catacattat ttacaattgg attattgttt tttgctataa gtattcttac aataaacctt
     3781 attgcagaac aaaatagttt ttcaacgtat aatatgggat ttgttttact tttctttttt
     3841 ggtcaaaatt ttttaaaaat ctcatttttc agatctacaa gtattttact agctatatta
     3901 ctcatttttg aaatatatac tctagcattt aaatatgttg ctacagatgt atttattaca
     3961 atctcatttt tcttatttgt cagttattta ttaagtactt tttcaagtta cttttttgaa
     4021 cttatcgacc gcaaaaacta ttggaatagt attcaacttc aaaaaacaaa cgaggaatta
     4081 aaaaatttac aaaaattaat ggaacaaaaa gttaccgaac gtactaatat gcttgaaaaa
     4141 gttgtaaaag aattacaaac agcaaaagca aaagcagaag aaagtaatat tataaaatcc
     4201 acttttttat caactatttc acatgaaatt agaactccat taactagtat tctaggattt
     4261 actcaattaa ttacaaaaac aaaaacctat gatgaaaaaa cacaagtata tgctgctaca
     4321 attgaaaaaa gtattgcaga attacttgat ataatgacca atatacttac actttcagaa
     4381 attcataatg acaggcttga aaaatcaaca accactattc atttcaaaac attaaaaaaa
     4441 caaatattag ccataagcga agaaattctt gcacgaagaa aaaagaattt aaaatttcaa
     4501 ataatcagca acgaacctgt tgaaatagaa attcctgttg gtgaaaaaga attacttatg
     4561 gtaataaaac aattaataga taatgcaata aaatatacag gagcaggaca ttttagtatt
     4621 gaatgcatta taaatgagtt gggtatgttc caaatttgtg taaaagatac cggcattggt
     4681 ataaatgcag aaaatttaca ttcaatattc gactttttta tacaagaaga tcaattagat
     4741 actcgcatgt ataatggtat aggtgttgga ttatctattt gtaaaggaat tataacaaaa
     4801 gcaggaggaa aaatttgggc agaatctgaa aaaaataaag gttcggcttt ttacgttacc
     4861 ataccaatag aatataatag taataatgaa taaataatga cggattatga atgcagttga
     4921 acaaaaatta gttgaatttt cagaaaaaat tattccatgg ttattaagtc atggtattaa
     4981 aattattgca ataattgtag tatcatatat tataaataaa attatttata aaattattga
     5041 ccgagccgtt cgtatagccg tagttggcga cagatattca tctcgcgaag cagaattgaa
     5101 acgtgaagaa acattaataa gcatatttac aacaactgta aaaattgctc ttattgtaat
     5161 agtatctctt atggtactta aagaaatagg cttagatatt gcaccattac ttgccggagc
     5221 aggaatagtt ggtttggctt taggttttgg tggtcaatat ttaattcgcg atatt
//
LOCUS       DAOTOW010000047         5249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13145_length_5249_cov_3.457643, whole genome shotgun sequence.
ACCESSION   DAOTOW010000047 DAOTOW010000000
VERSION     DAOTOW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13145_length_5249_cov_3.457643"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="P5243_01360"
     CDS             complement(<1..370)
                     /locus_tag="P5243_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18122.1"
                     /translation="MDPSDKKWLLTLNKNIQKHLPKIHSSICSEFTRHDSIEHFIHFY
                     LHSSGILYGYATKQMLCEFHNTQTWTYSDLYKISLTEGFYLIFFYLNQEKISSKENLS
                     LLLEEANNKLYEFYILYSITD"
     gene            complement(373..1056)
                     /locus_tag="P5243_01365"
     CDS             complement(373..1056)
                     /locus_tag="P5243_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18123.1"
                     /translation="MEISGFILKEEQLQNLESSIIPGTFVLETVDPFNGYYSSHPDEY
                     VPRTVFLITNKKYSKEAIARYSRTVCKNLQIELDAVYSEVTIQHELYYAIRVFDFKEY
                     SVIESIQRAFEKVGIEFKYSSLKIHSICRIKIFKVFMVHEDSKGIYSNLGKSKMFYFT
                     LPVELEFDEFKQLVQKVSNNWTGKSFNAAQGFFFRKQSAEDVVRIFSNHITDEMKHLL
                     VQKFLSFVQ"
     gene            1152..2462
                     /locus_tag="P5243_01370"
     CDS             1152..2462
                     /locus_tag="P5243_01370"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRS18124.1"
                     /translation="MSSLPEVSIFWFRRDLRLHDNVGLYYALKESKAVLPIFIFDTQI
                     LTQLQNPQDVRVNFIYSCVEYIQGVCKEFGSSLYVLYGNPIDVFSNLISQFSVVSVYA
                     NRDYEPYGIKRDASVELFLTQNGIAFYTFKDHVIFEQDEVVKQDGNPYTVYTPYAKTW
                     KMKFSQMQLQSYTSEQLLSHLFKTSVIPIPSLDSIGFSPTSVCSHIPQIPVDCIQNYH
                     KTRDFPAIEGTSQLGVHLRFGTVSIRELVCIAIQHNHVWLNELIWREFFSMILYHFPQ
                     VVHANFKSQYDSLQWINNEEHFQRWCDGETGYPIVDAGMRQLNETGFMHNRVRMITAS
                     FLCKHLLIDWQWGEAYFAQKLIDYELSSNNGNWQWAASTGCDAVPYFRIFNPYEQTKR
                     FDAEYLYIQKWVPNYNSSTYIKPIVEHSFARQRALQTYKKCTKF"
     gene            2588..3853
                     /locus_tag="P5243_01375"
     CDS             2588..3853
                     /locus_tag="P5243_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRS18125.1"
                     /translation="MQLIDNYNFAGKKAIVRVDFNVPLNKETGEVSDDTRIRGALPTI
                     NKILQDGGSVILMSHMGRPKQNPDPKCSLQQIIPAVSKNLGGKEVKFVADCMKAHEAA
                     ANLKPGEVLLLENLRFYEEEEGKPRLAEDASEEEKAAGKARVKASQKEFVKTLASYAD
                     VYVNDAFGTAHRAHGSTALIADLFSTENKMFGYLINSELAAMDKVLQSKDAPKTAIMG
                     GAKVSDKIQIISRLLPTIDNLIIGGGMTYTFIKAQGGNIGNSLCEADKLDLALDILKK
                     AKEHNVNVYIPVDAVNADKFDPNANTNISKTNETPDGWMGLDIADETIAMFSEVIAKS
                     KIVLWNGPMGVFEMDKFSKGTSAIAQALAKATANGAFTLIGGGDSVAAINKNKLADKV
                     SYVSTGGGAMLEYMEGKVLPGIAAIRGDK"
     gene            4094..4396
                     /locus_tag="P5243_01380"
     CDS             4094..4396
                     /locus_tag="P5243_01380"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HRS18126.1"
                     /translation="MKKQFAKNNLECKVTFQIPAEMLAGSKIASVAGDFNGWNTEANP
                     VKITKGVGSVAITLPVGKEFQYKFVIDGNRWENDPEADRYVSNEFGEANSIVETYN"
     gene            4704..5162
                     /locus_tag="P5243_01385"
     CDS             4704..5162
                     /locus_tag="P5243_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18127.1"
                     /translation="MFLFVISCIGLHAQESVNASGGKATGSGGSASYSVGQVVYTTIL
                     GTNGSVTQGIQQPYEISVLSTIEEAQGIRLVATAYPNPTVNELTLKIDAFDISNVSYL
                     LYNNNGRVLQREPILHYITSIDMSKYTPSIYYVKIIQGDTEIKTFKIIKH"
BASE COUNT         1833 a          727 c          894 g         1795 t
ORIGIN      
        1 aatctgttat tgaatataat atataaaatt catatagttt attgtttgct tcttctaata
       61 gtaatgacaa attttctttt gatgatattt tctcttggtt caaataaaaa aatattaaat
      121 aaaaaccttc ggtaagacta attttataga ggtcagagta tgtccaggtt tgcgtgttgt
      181 gaaattcaca caacatttgt tttgtagcat atccgtataa tatgcccgat gaatgtaaat
      241 aaaaatgaat aaaatgttct attgaatcat gtcgagtaaa ttcactacat atagaagagt
      301 gaattttagg taaatgtttt tgaatatttt tgttaagggt aagtaaccac ttcttgtctg
      361 atggatccat atttactgta caaatgacaa aaatttttga accaacagat gcttcatttc
      421 atcggttatg tgattgctaa atattctcac aacatcttcg gccgattgtt tcctgaaaaa
      481 gaacccttgt gcagcgttga aacttttacc ggtccaattg tttgaaactt tttgaaccaa
      541 ttgtttaaat tcatcaaatt ccaattcaac cggtaatgta aaataaaaca tttttgattt
      601 tcctaaattg ctgtaaattc ctttggaatc ttcatgcacc ataaacactt taaatatttt
      661 tattctacaa atagaatgta tttttagtga tgaatattta aattctattc caactttttc
      721 aaaagctctc tgaatagatt ctattacaga atattctttg aaatcaaata ctcgaatagc
      781 ataatataat tcatgttgta ttgtaacttc cgaatatacg gcatctaatt ctatttgcag
      841 atttttacaa acagttctgc tatatcgggc tattgcttct tttgaatatt ttttgtttgt
      901 aattaaaaaa acagtacgag gaacatattc gtcaggatga ctactgtaat atccgttaaa
      961 tggatcaacc gtttcaagaa caaatgtgcc gggaattata gatgattcta aattctgtaa
     1021 ttgttcttct tttaatataa aacccgagat ttccatatct gtattttttc taatttagac
     1081 tgcgaaactt ttgctaaatt acataaaatt agttacaatt gctactataa ctttttaaaa
     1141 aaatatgatg tatgtcatca cttcccgaag tttcaatttt ttggtttcgc cgcgatttgc
     1201 gtttgcatga caatgttggt ttatattatg cgttgaaaga atcaaaagca gttttaccta
     1261 tttttatttt tgatactcaa attttaaccc aattacaaaa tccccaagat gtacgtgtga
     1321 attttatcta ttcatgtgtt gagtatatac aaggtgtttg taaagaattc ggatcgtctt
     1381 tgtatgtttt gtatggtaat ccaattgatg ttttttcaaa cttaatttct caattttctg
     1441 tagtttctgt ctatgccaat agagattatg aaccctatgg aatcaaacgt gatgcttctg
     1501 ttgaattatt cttaacccaa aacggtattg ctttttatac ttttaaagat catgtaatat
     1561 ttgaacaaga tgaggtagtg aaacaagatg gaaatccata tactgtatat acaccatatg
     1621 caaaaacgtg gaaaatgaag ttcagtcaaa tgcaattaca atcatataca agcgaacaat
     1681 tattgtcaca tttgtttaag acctctgtaa taccgattcc ttcattggac tctataggat
     1741 tttcaccaac tagtgtttgt tcacatattc ctcaaatacc tgtagattgt attcaaaatt
     1801 atcataagac tcgtgatttc cctgcaatag aaggaacttc tcaattgggg gttcatctgc
     1861 gatttggtac agtaagtatt cgtgaattag tttgtatagc aatacaacat aatcacgtat
     1921 ggttgaatga attaatttgg agggaatttt ttagtatgat tttatatcat tttccacagg
     1981 tagtacatgc taatttcaaa tcacaatatg attctttaca atggataaat aatgaagagc
     2041 attttcaaag atggtgcgat ggagaaaccg gatatccaat tgtagatgcc ggtatgcgac
     2101 agttaaatga aaccggattt atgcataata gggtacgtat gattactgca agttttttgt
     2161 gtaaacattt gcttatagat tggcagtggg gcgaagcata ttttgcacaa aaacttattg
     2221 attatgaatt gtcttcaaat aatgggaatt ggcaatgggc agcaagtaca ggttgcgatg
     2281 ctgttccgta tttcagaatt tttaatccct atgaacaaac aaagcgtttt gatgcagaat
     2341 atttgtatat ccaaaaatgg gtgccaaatt ataactcaag tacctatatt aagcctattg
     2401 tagagcattc gtttgctcga caacgtgcat tacaaactta taagaaatgt actaagtttt
     2461 aaacggtaat aaggcaaaac aataaaacaa ttaaataaaa aatatccttt aatataaaat
     2521 aatcatttca ttttttttat atttggactc gttaaaaaaa tttgtaatca ttaaaaaata
     2581 ttaaacaatg caattaattg ataattataa ttttgcaggc aaaaaagcaa ttgtgcgtgt
     2641 tgactttaat gtgcctttga ataaagaaac aggtgaagtg tctgatgata ctcgtatccg
     2701 tggtgcatta cctaccatta ataaaatatt acaagatggt ggttcggtaa ttcttatgtc
     2761 gcatatgggg cgaccaaaac aaaatcccga tcctaaatgt tcattgcaac aaattattcc
     2821 tgctgtttct aaaaatttag gtggtaaaga agtaaagttt gttgctgatt gtatgaaagc
     2881 acatgaagca gcagcaaatt taaaaccagg tgaagtgttg ttgctggaaa atcttcgttt
     2941 ttatgaagaa gaagaaggta aaccacgttt ggccgaagat gcttctgaag aagaaaaagc
     3001 tgccggtaaa gctcgtgtaa aagcatctca aaaagaattt gtaaaaacgc ttgcaagtta
     3061 tgctgatgtg tatgtaaacg atgcatttgg aacagctcac cgagctcatg gttctacggc
     3121 attaattgca gatttatttt caaccgaaaa taaaatgttt ggatacctta taaacagcga
     3181 acttgctgct atggataaag tattgcaatc aaaagatgct cctaaaactg ctattatggg
     3241 gggggcaaaa gtatcagata aaattcaaat tatatctcgt ttattaccta ctatagataa
     3301 tttaatcatt ggtggtggta tgacatatac gtttatcaaa gcacaaggcg gaaatattgg
     3361 taattcattg tgcgaagccg ataaacttga tttagctctt gatattttga aaaaagcgaa
     3421 agaacataat gtaaatgtat acataccggt agatgctgta aatgctgata aatttgatcc
     3481 aaacgcaaat acaaatattt ccaaaacaaa tgaaactcct gatggttgga tgggattgga
     3541 tattgcagat gaaactattg ctatgttttc tgaagtaatt gcaaaatcaa aaattgtatt
     3601 atggaatggt ccaatgggag tattcgaaat ggataaattc tcgaaaggaa cttcggctat
     3661 agcacaagcg cttgctaaag ctacagcaaa cggtgcgttt actttaattg gtggtggtga
     3721 ttctgttgcc gctatcaata aaaataaatt agctgataaa gtgagctatg tttcaaccgg
     3781 tggtggtgct atgttagaat atatggaagg taaagtattg ccgggaatag ctgccataag
     3841 aggtgataaa taatttaaaa actgagatta ttataaaaaa ctcctgtaaa gattttttat
     3901 aggagttttt tattttttaa agattaaaaa aaaacgtgca tatatttgat ttgcattttt
     3961 ttacaagaat gatgatgtaa atcaaatgtt aagaataagt aaaattatgt atttttcttt
     4021 ttaatttaaa aaaatactag tatttttgat tatataaata ctaaactacc aatataaact
     4081 aaaacataat tgtatgaaaa aacaatttgc aaaaaacaat ctcgagtgta aagttacatt
     4141 tcaaattccg gcagaaatgc ttgcaggttc aaaaatagca tcagtagcag gagattttaa
     4201 tgggtggaat actgaagcaa atcctgtaaa aattacaaaa ggtgtaggct ctgttgctat
     4261 tacgttgcct gtgggtaaag aatttcaata caaatttgta attgatggca atcgttggga
     4321 gaacgatcct gaagcagata gatatgtttc aaatgagttt ggtgaagcaa attctatagt
     4381 tgaaacatat aattaatatc tcaattccat agataaatgt gaatatacta ttttagtatg
     4441 tattaataat attgtgtgaa aagctaccaa aaattggtag ctttttttgt gtataaagga
     4501 acaatttcat tcttagattt tttacaaaat aatttttctc taaccgaatt gaatttttag
     4561 ttatttaaaa atcaattgat tatgtttttt tgcaattagg ttttattttt tttatttaaa
     4621 atgaaactgt aattcagatt ttacgtacat atatttattt aattagtata aatatgcacc
     4681 acaatcaatt gaaaatacag ataatgttct tgtttgtaat tagttgtata gggttacatg
     4741 cacaagaaag tgttaacgca tcaggcggta aggcaaccgg aagcggaggt tcggcaagtt
     4801 actctgttgg acaagtagtg tataccacca ttttgggaac aaatggcagt gtaacacaag
     4861 gaatacaaca gccatatgaa atttctgttt taagcacaat agaagaagcg caaggaatta
     4921 ggcttgtggc gaccgcgtat ccaaatccca ccgtgaatga actaacgtta aagatagatg
     4981 catttgatat ttcaaatgta tcgtatttac tttataataa taatggaaga gtattgcaac
     5041 gagaacctat attacattat ataacaagta tagatatgag taaatataca ccttcaatat
     5101 attatgtaaa gattattcaa ggagatacag aaattaagac attcaaaata attaaacact
     5161 aatgcgtatg aaaaagtttt acaccttagc agcagccatt ttagtaacta tggcaaccgt
     5221 gtttgctcaa tcacccgata aaattagtt
//
LOCUS       DAOTOW010000048         5249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13146_length_5249_cov_3.135926, whole genome shotgun sequence.
ACCESSION   DAOTOW010000048 DAOTOW010000000
VERSION     DAOTOW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13146_length_5249_cov_3.135926"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="P5243_01390"
     CDS             complement(<1..526)
                     /locus_tag="P5243_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18128.1"
                     /translation="MKYIQAKIVILFVCVFGFSIHTLFSQTDTYTWSNLPLGGGGFVS
                     AVIAHPKTQNVIYARTDVGGAYRWVNVTKTWVPLNDWCSIDELGLLEIESLALDPNNP
                     AKLYMLAGTSYFNNGKTAILISNDYGATFTVVDVSNQFKAHGNGMGRQTGEKLAVDPK
                     NSNILFCGTRRDGLF"
     gene            complement(659..5227)
                     /locus_tag="P5243_01395"
     CDS             complement(659..5227)
                     /locus_tag="P5243_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS18129.1"
                     /translation="MGSSETVHVSYFPTNVTDSSLTWTSSDASIVNVSQQGIISAVGL
                     GTATITVTSVNNKTATFTVQVNPIVATSISLNTNAVTLTTGNTQQLIATIEPLNTTNK
                     TVVWTSSATSIAQVNSNGLITAVNQGNAIITATTSNGLTATCNVTVELVSIPVSSVAL
                     NKKTASIQLSQTETLVATVMPLDATNKTIVWSSSNSSIATVSQSGIITPVAVGQTNII
                     ATASNGKADTCVVTILHNPISTIGFSQTTVNVSEGATVNLLQLLVTQPSNAFIQSVVW
                     SESSSNASISAQGVLTNSLQYGVETVTVTATITDAYGTQFIKTIDVVLHGCAVQLSSV
                     TVSPSTVSIVENQTLQLIPLYSPANACIMSVSYASNAVHIATVSASGLVSAVAPGTAI
                     ITITVNDGFTTKTVTKEITVLKDCSGPIVLSLPATMQIVYGQSATITPTFTPNNECTA
                     NKNITWTSSNPAVASVNNGVITTNAIGTTIITASTDGTGTTSATCTITVVSDCNAGNV
                     LLQLNTNTITMYKNETSLITTTITPANACNTNIVWQSNNQSVATVVNGLVTAIGIGSA
                     TITATSEQTPSQVKTVSVQVIERLPSSISMPSTATVFVGNTLQLTPSILPANADNKSI
                     TWTSSNTAIATVSTSGIISGLNAGTVTITATTVNNLSAQCIVTVSTIPVESVTLNKEQ
                     HSVQVYEQFELQATVLPNYATNKQLAWTSSDVSVATVSNGIVTALKAGTAVIRASSIN
                     NMYDECIVTVTDIYPTSLNLTVNLPSMNVGTVQTIEVSYSPANATNTSFIWSSNNSSV
                     ITVNQLGQITAISPGTATITASTATGVSKSITITVNAVLATSLQLNTNTATLKELENQ
                     QLIALILPENTTNKTITWTSSNPSVATVDNTGKVTALAVGTAEITATTSNGIQAKCVV
                     NVVLFTIPVSSIQLNKESTSILLGNTEQLIATVAPENATNKTVVWKSETTSIATVSQS
                     GIVTSVGVGAVRIIATASNGMADTCIVYVQPIMAQSITIDQTNISMTVGGTLQVSATI
                     LPANTTNKSVTWLTSNSNIVSVSEQGLLTAKAIGVVTLYAQTYNGLIATREITVENNV
                     IEIQSISLPSNIEIDIDESIVLQPQFTPANATNTSLTWTVGNNQIALVNALGKVTGLS
                     AGTTVVTATSSNNKQVSVIVSVKPLEAEEVQIIPSTVSLHIQETAQLQVSVLPIKTTD
                     KSVTWTSSNPSVATVNQGIVTAKTIGQTTVYAITKNGKVGECIVHVVPVEAEAVQISV
                     SDVAIMVSQTFQLTAQILPSNVSNTSIQWSSSNQGIVCVDNTGKITANAIGKAYIYAT
                     ANNGLKDSVEVTVSILNYAPEITSIPEQKIRVGQTFTAINLQNYVTDDNTDVNSLLWA
                     ANTSGVLSMNITSKGIATIYNSNPLWIGSQTITIYCTDEQGLQSSVDIIFTVEPKVHI
                     ETISILSVQAYPNPAKDNVRISFTAAKPELYSITIVSMSGNIVMQEREWVSNQYSHVF
                     DVSLLPRGMYMIQIATEDEQKIVPMILY"
BASE COUNT         1645 a          908 c          863 g         1833 t
ORIGIN      
        1 taaataaccc atctctgcga gttccacaaa ataaaatatt actattttta gggtcaacag
       61 ctaatttttc gccagtttgc cgccccatac catttccatg agctttaaat tgatttgaaa
      121 catcaacaac agtaaaagta gcaccataat catttgaaat taatattgca gttttgccat
      181 tattaaaata actagtacct gcaagcatat ataattttgc aggattatta gggtctaatg
      241 ccaaactttc aatctccaac aatccaagtt catcaatact acaccaatca tttaaaggta
      301 cccaggtctt tgttacattt acccatcggt aggcaccacc aacatcggta cgtgcataaa
      361 ttacattttg tgttttggga tgtgctatta cagcagaaac aaaaccacca ccacctaatg
      421 gtaaattact ccatgtatat gtatcggttt gtgaaaatag tgtatgaatt gagaatccaa
      481 aaacgcacac aaataaaata acaatttttg cttgaatata tttcataata aaaacagttt
      541 acgcatattt gtggtaaata tacaaaaaca aaaaaaacct cgcaattttt tacgaggttt
      601 ttttaattat aacttattta ttttcaaact attgttagca aatccaacaa taatgtactt
      661 aatacaatat cataggtaca attttttgct cgtcttcagt tgcaatttga atcatataca
      721 tacctcttgg caataatgat acatcaaaca catgtgaata ttgatttgat acccactctc
      781 tttcttgcat tacgatattt cctgacattg aaactatcgt tattgaatat aattcaggtt
      841 ttgctgcagt aaaagaaatt ctgacattat cttttgcagg atttggatat gcctgaaccg
      901 aaagtattga gattgtttct atatgaactt tgggttctac ggtaaatata atatctacag
      961 aactttgcaa tccttgctca tcagtgcaat aaatagtaat agtttgacta ccaatccaca
     1021 atggattact attgtatata gttgcaatac ctttacttgt aatattcata cttaaaactc
     1081 cagatgtatt tgctgcccat aacaaagaat ttacatcagt attatcatcg gtaacataat
     1141 tttgtaagtt aattgcagta aatgtttgac caactctaat tttttgctcg ggtattgaag
     1201 taatttcagg tgcatagttt aatatagaaa ctgtaacttc aacagaatct tttaatccat
     1261 tatttgcagt agcataaata tatgcctttc cgatagcatt tgcagtgatt ttacccgtat
     1321 tatcaacaca tacaatacct tgatttgaag atgaccattg aattgatgta ttagatacat
     1381 ttgaaggcaa aatctgagct gtcaattgaa acgtttgcga taccataata gcaacatcac
     1441 ttacagatat ttgtactgct tctgcctcaa caggaactac atgcactata cattcgccta
     1501 cttttccatt ttttgtaata gcatacaccg ttgtttggcc tattgttttt gccgttacaa
     1561 taccctgatt tactgttgcc actgaaggat ttgacgatgt ccaagttaca gatttatcgg
     1621 tagttttaat aggcaaaaca gaaacttgca gttgtgcagt ttcttgaata tgcaatgaaa
     1681 cagtagatgg tattatttgt acttcctcag cttcaagtgg ttttaccgaa acaattacag
     1741 aaacttgctt gttgttagaa cttgttgcag taactaccgt agtacccgca cttaatccgg
     1801 ttacttttcc caaagcatta actaaagcta tttgattatt acctactgtc cacgttaatg
     1861 aagtatttgt agcatttgcc ggtgtaaatt gcggttgtaa aacaattgac tcatctatat
     1921 ctatttctat attagagggc aatgaaatac tttgaatttc tattacatta ttttctactg
     1981 taatttcacg agttgcaatt aatccattat acgtttgagc atataaagta acaacaccta
     2041 ttgcttttgc tgttaacaaa ccttgttcag aaacactaac aatattagaa ttagaagtaa
     2101 gccaagtaac tgatttattt gtagtattag caggtaatat agttgctgaa acttgtaacg
     2161 ttcctccaac agtcatacta atatttgttt gatctatggt aatactttga gccattatag
     2221 gttgcacata tacaatgcac gtatctgcca taccattaga tgcggtagca attatacgaa
     2281 cagctccaac tcctacacta gtaactatac ccgattgact taccgttgct atacttgtgg
     2341 tttcgctttt ccatacaacc gttttattcg ttgcattttc cggtgctact gttgcaatta
     2401 actgttctgt atttccaagt aatattgatg tagattcttt atttaattgt attgatgaaa
     2461 caggaattgt aaataacaca acatttacaa cacacttagc ttgaattcca tttgaagtag
     2521 ttgcagtaat ttctgcagta cctactgcta aagctgttac ttttccggta ttatctacgg
     2581 tagctacaga aggattactt gatgtccatg taatagtttt atttgtagta ttttcaggta
     2641 aaattaatgc aattaattgt tgattttcta actctttaag cgttgcagta ttggtattga
     2701 gttgtaatga tgttgctagc acagcattaa cagtaattgt aatagatttt gaaacacctg
     2761 ttgcagttga tgctgttata gtagcagtac ccggacttat tgcagtaatt tgtcccaatt
     2821 gattaaccgt aattactgaa ctattatttg aagaccatat aaaagaagta tttgtagcat
     2881 ttgccgggct atatgaaact tcaatagttt gtacggttcc tacattcatc gatggtagat
     2941 taactgtaag atttaaagat gttgggtaaa tatcggttac cgtaactata cattcatcat
     3001 acatattatt aattgatgaa gctcgtatga cagcagttcc agctttaagt gctgtaacta
     3061 taccattaga cacagttgca accgacacat cactgctagt ccatgccaat tgtttattgg
     3121 tagcatagtt tggcaataca gtagcttgca gttcaaattg ttcataaact tgtacagaat
     3181 gttgttcttt atttaaagtt acagattcta caggtatagt tgaaactgtt actatacatt
     3241 gagcactaag attatttaca gtagttgctg ttatagtaac tgttcccgca ttgagtccac
     3301 taataatgcc tgaagtacta accgtagcaa ttgcagtatt cgaagatgtc catgtaattg
     3361 atttattatc agcatttgca ggtaaaattg aaggagtaag ttgaagtgta tttcctacaa
     3421 atactgttgc tgtagagggc atgctaattg aagacggtaa tcgttctatg acttgaactg
     3481 ataccgtttt aacttgagaa ggtgtttgtt cacttgttgc agtaatagtt gccgaaccaa
     3541 tacctattgc tgtaactaag ccatttacta cagtagcaac cgattgatta tttgattgcc
     3601 aaactatatt agtattacat gcatttgccg gagtaatagt agtagtaatt aaacttgttt
     3661 catttttata cattgttata gtattggtat ttaattgcaa caatacatta ccggcattac
     3721 aatcgcttac aacagtaata gtacaagtag ccgatgttgt tccggttcca tctgtagatg
     3781 ctgtaataat agttgttcct attgcatttg ttgttattac accattgttt accgatgcaa
     3841 cagccggatt tgaacttgtc catgtaatat ttttatttgc agtacactca ttatttggag
     3901 taaacgtagg tgtaattgtt gctgattgcc catacactat ttgcattgtt gcaggcaacg
     3961 aaagaacaat aggaccagaa caatctttta acacagtaat ttctttggtt actgttttag
     4021 tagtaaaacc atcgtttact gtaatagtaa taatagctgt accgggagca actgcagaaa
     4081 ctaatccaga agctgatact gttgcaatgt gtacagcatt acttgcatat gaaaccgaca
     4141 ttatacatgc atttgcaggt gaatataaag gaataagctg taaagtctga ttctctacaa
     4201 ttgatactgt tgatggtgat acagttacag aacttagttg cacagcacag ccatgtaata
     4261 caacatcaat agtttttata aattgcgtac catatgcatc agttatagtt gctgttaccg
     4321 taacagtttc aactccatat tgcaaagaat ttgttaacac accttgtgca gaaatactcg
     4381 cgttactact cgattcactc cataccacac tttgtataaa cgcattactt ggttgagtta
     4441 ccaacaattg taataaattt actgtagctc cttctgacac atttactgtt gtttgtgaaa
     4501 aaccaattgt tgatattgga ttatgcaata ttgttacaac acaagtatca gctttaccat
     4561 tagatgctgt agcaataata ttagtttggc ctacagctac cggagtaata attccggatt
     4621 gactaactgt ggctatactt gaattagatg acgaccaaac tattgtctta tttgtagcat
     4681 caagaggcat tactgttgcc actaaggttt ctgtttgcga taattgaatg cttgcagttt
     4741 ttttatttaa tgcaacagaa cttaccggaa tacttactaa ttctaccgta acattacatg
     4801 ttgctgttaa accatttgat gtagtagctg taataattgc attgccctga ttaactgcag
     4861 taattaatcc attactattt acttgggcaa tactagtagc gctagaagtc caaacaactg
     4921 ttttatttgt tgtatttaaa ggttctatag ttgcaattaa ttgctgtgta ttaccggtag
     4981 taagtgtaac tgcatttgta tttaatgata tagatgttgc tacaatagga tttacctgta
     5041 ctgtaaatgt tgccgtttta ttatttaccg aagtaaccgt aattgttgct gttcctaatc
     5101 caactgccga aataatacct tgttgcgaga cattaactat agatgcatct gaagatgtcc
     5161 atgtaagaga agagtctgtt acatttgttg ggaaataact tacgtgcact gtttctgaac
     5221 taccaatata acgagaaact gtacctgta
//
LOCUS       DAOTOW010000049         5228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13221_length_5228_cov_3.082931, whole genome shotgun sequence.
ACCESSION   DAOTOW010000049 DAOTOW010000000
VERSION     DAOTOW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13221_length_5228_cov_3.082931"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="P5243_01400"
     CDS             complement(<1..1619)
                     /locus_tag="P5243_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRS18130.1"
                     /translation="MTQLQKQIINLNNVTIRFTGDSGDGMQLVGTLFSDTSALFGNDV
                     STFPDYPSEIRAPQGTVAGVSGFQVHIGHNKIHTPGDYCDVLVAMNPAALKANLKWAK
                     RGATIIVDADTFDDKHLQKAGYVTNPLTDNSLQPYNLICAPISSQTHACLSDTQLDNK
                     SIMRCKNMFALGITYSLFNRPFEHTFTYFEEKFAKYPALIEANKKVLREGYIFAENTH
                     AMPSASIEPAKIEKGKYRNMNGNTAVAWGLMAAAEKSQLPLFLGSYPITPATDILQEL
                     AKHKNLGVKTFQAEDEIAGICTAIGAAYAGNLAVTTTSGPGLALKTEAIGLACMMEIP
                     IVIVNVQRGGPSTGLPTKTEQADLMQAIYGRNGEAPCIVIAATTPSDCFYYAYIAAKL
                     TLEHMTPVILLTDGFLANGSQPWKVITMNELPPITPRLQTQKLSEHTPFMRNDDTLAR
                     NWVIPGTPELMHRIGSLEKDFVTGQASHVPENHHKMVTLRKEKVEKVVRVIPPLHTEG
                     EEYGDLLVVGWGGTYGHLLSAVQELLLEGKH"
     gene            complement(1871..2821)
                     /gene="ftsY"
                     /locus_tag="P5243_01405"
     CDS             complement(1871..2821)
                     /gene="ftsY"
                     /locus_tag="P5243_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRS18131.1"
                     /translation="MGLFDFFKGEKKEILDKGLEKTKESVFKKITRSIAGKSKVDDEV
                     LDELEEILITSDVGVQTTIKIIERIEARVSRDKFLGTNELNTILKEEITALLTENTND
                     ISDSFVEHLPKPYVIMVVGVNGVGKTTTIGKLAHKFKQAGKIVYLGAADTFRAAAVDQ
                     LTIWAERVQVPIVKQQMGSDPASVAYDTLSSAVANNADVVIIDTAGRLHNKVNLMNEL
                     SKIKRVMQKIVPNAPHEILLVLDGSTGQNAFEQAKQFTLATEVNALAITKLDGTAKGG
                     VVIGISDQFKIPVKYIGVGEGINDLQFFRKKEFVDSIFGE"
     gene            complement(2987..3139)
                     /locus_tag="P5243_01410"
     CDS             complement(2987..3139)
                     /locus_tag="P5243_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRS18132.1"
                     /translation="MAKKVVASLQKGAGKEFTKVVRCIKSEKTGAYTFKEEMVPNAMV
                     QSVLSK"
     gene            complement(3149..3331)
                     /gene="rpmG"
                     /locus_tag="P5243_01415"
     CDS             complement(3149..3331)
                     /gene="rpmG"
                     /locus_tag="P5243_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRS18133.1"
                     /translation="MAKKGNRVQVILECTEHKESGMPGTSRYITTKNRKNVPDRMELK
                     KYNPILRKMTIHREIK"
     gene            complement(3344..3586)
                     /gene="rpmB"
                     /locus_tag="P5243_01420"
     CDS             complement(3344..3586)
                     /gene="rpmB"
                     /locus_tag="P5243_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRS18134.1"
                     /translation="MSRVCQITGKTAMSGHNVSHSNTKTKRVFDVNLFDKKYYLAEED
                     RWVNLRVSANGIRTINKKGLLNALKDAQAKGYIKSF"
     gene            complement(3747..4961)
                     /gene="rocD"
                     /locus_tag="P5243_01425"
     CDS             complement(3747..4961)
                     /gene="rocD"
                     /locus_tag="P5243_01425"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRS18135.1"
                     /translation="MTPQDYMAREDKYGAHNYHPLPVVLEKGEGVYMWDVEGKKYYDF
                     LSAYSAVNQGHCHPKIINALIEQSKKLTLTSRAFYNNVLGEFEEYVTKYFGYDKVLPM
                     NTGAEADETALKLARRWGVKKKGIANDDVKIICCEGNFHGRTITIVSMSNDPDSFANY
                     GPLTPGFIRIPYNDTKALEEVLEKHGKTVAGFLVEPIQGEAGVYVPDSGFLKKSYELC
                     KKHNVLFIADEVQTGLARTGKMLACDHEGVRPDILILGKALSGGVFPVSCALADDEIM
                     LCIKPGEHGSTYGGNPVAGKVAIAALEVLKEENLCENSERLGEIFRAELNKINHPMKK
                     IVRGKGLLNAIVIEPHKGKTAWDFCLALKENGLLAKPTHEHIVRFAPPLIINEAQIRE
                     CVAIIEKTFNQF"
BASE COUNT         1512 a          908 c          840 g         1968 t
ORIGIN      
        1 atatgtttgc cttcgagtaa taattcttga actgccgata ataaatgtcc gtatgtgcct
       61 ccccaaccta caactaataa atcaccatat tcttcacctt cagtatgtaa aggaggaatt
      121 acacgtacaa ctttctcaac tttttctttt cgcaaagtta ccattttatg atgattttcg
      181 ggcacatgcg atgcttgtcc tgtaacaaaa tctttttcta aacttccaat tctatgcatt
      241 aattcgggag tacccggtat aacccaattg cgagccaatg tatcatcatt acgcataaaa
      301 ggtgtatgtt ccgataattt ttgtgtttgt aatcgagggg taataggagg taattcattc
      361 atagtaatta ctttccatgg ttgtgaaccg tttgctaaga atccgtcggt taacaatatt
      421 accggagtca tgtgttctaa tgttaatttt gctgctatat atgcgtaata aaagcaatca
      481 gatggagtag tggctgctat tacaatacaa ggagcttcac cgtttctacc ataaatagct
      541 tgcattaaat ctgcttgttc tgttttggta ggtaatcccg ttgacggtcc ccctcgttgc
      601 acatttacta ttacaatggg tatttccatc atacaagcca atcctatagc ttctgtttta
      661 agagcaagtc cgggacctga agtagtagta acagctaaat tacctgcata tgctgcacct
      721 atagctgtgc atataccggc tatttcatct tcggcttgaa aggttttaac tcccaaattt
      781 ttatgttttg ctaattcttg taaaatatcg gtagcaggag taatagggta tgaacctaaa
      841 aataatggca attgagattt ttcggcagct gccataagtc cccacgctac agcagtatta
      901 ccgttcatat ttctgtattt acctttttct atttttgcag gttctatact tgccgatggc
      961 atagcatgtg tgttttctgc aaaaatgtat ccttcgcgaa gtactttctt gtttgcttca
     1021 attaacgcag gatattttgc aaatttttct tcaaaatatg taaaggtatg ttcaaatggt
     1081 ctattaaata aactgtaggt aatacctaat gcaaacatgt ttttgcatcg cattatgctt
     1141 ttattgtcga gttgtgtatc gcttaagcac gcatgtgttt gtgatgatat tggagcacaa
     1201 ataagattgt atggctgtaa tgaattgtcg gttaacggat tggttacata tcctgctttt
     1261 tgcagatgtt tgtcgtcaaa tgtatcggca tctactataa tagtagcacc tcgttttgcc
     1321 cattttaaat tagcttttaa tgctgccgga ttcatagcaa ccaatacatc gcaatagtca
     1381 ccgggtgtat gaattttgtt gtgccctata tgaacctgaa aacctgaaac tcctgctaca
     1441 gttccttgtg gtgcacgtat ttccgatggg taatcgggaa atgtagaaac gtcgttaccg
     1501 aataatgccg aagtgtctga aaacaatgta cctactaatt gcattccatc gcctgaatct
     1561 cctgtaaaac gaatggttac attgtttaaa ttaattattt gtttttgtaa ttgtgtcatt
     1621 acagtagtat ttattttttt taaatgtaat cacaaaacag tgtatgattt atcaatacac
     1681 ataattattc cttgttgttc caaattatac aatttggttt tatttggatt acaaaagtac
     1741 ttttttatat ggtaatttca taattcaaca atagtaacat gttttttatg agcatgaaat
     1801 aatatatata aaaatatgat gaatttaaga gtaggaaaga ggtgtatagg tattgataat
     1861 aaaaattgtt ttattcacca aatatagaat caacaaattc tttttttcta aaaaattgta
     1921 aatcatttat accttcgcca acaccaatat attttaccgg tattttgaat tgatcagaaa
     1981 ttcctattac aacaccacct ttagctgtac cgtctaattt tgtaattgct aaagcattta
     2041 cttcggttgc caatgtaaat tgttttgctt gttcaaatgc attttgcccg gtagaaccat
     2101 cgagaaccaa taggatttcg tggggagcat tgggaacaat tttttgcatt acacgtttta
     2161 tttttgataa ttcgttcata aggtttactt tgttgtgtaa tcgtcctgca gtatctatta
     2221 ttactacatc ggcattgttt gctacggccg aacttagggt atcataagct accgaagcag
     2281 ggtcagaacc catttgttgt tttactattg gtacttgtac acgttcggcc catattgtaa
     2341 gttggtcaac agccgctgct ctaaatgtat cggcagcacc taaataaaca attttacctg
     2401 cttgtttaaa tttatgcgct aatttaccaa ttgtagtagt ttttccaaca ccatttactc
     2461 ctactaccat tattacatat ggtttaggta agtgttcaac aaatgaatct gaaatatcat
     2521 ttgtattttc agtaagcagt gcagtaattt cttcttttaa aattgtatta agttcattgg
     2581 tgcctaaaaa tttatcgcga gatacacggg cttctattcg ttcaattatt ttaatagttg
     2641 tttgaacacc aacatcagag gttataagaa tttcttctaa ttcgtcaagt acttcatcat
     2701 ctactttaga tttaccggct atacttctgg taattttttt aaaaacagat tctttggttt
     2761 tttctaaccc tttatctaaa atttcttttt tttcgccttt aaaaaaatca aataagccca
     2821 ctgttctgaa ttttatttag tattaatcta tgatttgcaa atatagaaat atttatgaat
     2881 catttataat aatactgttt ttcaaaaatg ataatgaagt tctgaaaata aaaaaccctt
     2941 atcaaataac ttttgataag ggttttaaat tatttgataa aaaatattat ttactcaata
     3001 cagattgtac cattgcattt ggcaccattt cttctttgaa tgtgtatgct ccggtttttt
     3061 ctgattttat acaacgaact acttttgtaa attctttacc ggctcctttt tgtaacgatg
     3121 caacaacctt ctttgccata gtatataatt atttaatttc tctgtgaata gtcatttttc
     3181 ttaatatggg attgtatttt ttcaattcca ttctatccgg tacattttta cggtttttag
     3241 ttgtaatata tcgcgatgtt ccgggcattc cgctttcttt gtgctctgta cactcaagaa
     3301 taacttgtac tctgttacct ttttttgcca ttgtttcctt gtattagaaa gatttaatat
     3361 atccttttgc ttgtgcgtct tttaacgcat ttaataagcc ttttttattg attgttctaa
     3421 taccattagc tgatactcgt aagtttaccc aacgatcttc ttctgctaaa taatattttt
     3481 tatcgaacaa attcacatca aaaactcgtt tagtttttgt attggaatgc gatacgttat
     3541 gacctgacat cgctgttttt cctgttattt gacaaactct tgacatttct gttatttatt
     3601 tttgtaacat tctttaaaat cgaggtgcaa aataatggaa ttttaaagta acaaccaaac
     3661 tatttaacac tttttttttt gtttttgtga attttgtgat gtttatatat ataacgggag
     3721 ttatagctaa ctcccgttat tgatttttag aattgattaa atgttttttc aattattgca
     3781 acacattcac gtatttgagc ttcgtttatg ataagtggtg gagcaaaacg tacgatatgt
     3841 tcgtgggtag gttttgcaag caaaccgttt tctttgagtg ccaaacaaaa atcccatgct
     3901 gtttttcctt tgtgaggttc tataactata gcattcaata atcctttacc acgtacaatt
     3961 tttttcattg ggtggtttat tttatttaat tctgctcgga aaatttcacc taatcgttca
     4021 gaattttcac ataaattttc ttcttttaat acttctaacg ctgctattgc aactttacct
     4081 gcaactgggt taccgccata ggttgagcca tgttctccgg gttttatgca caacattatt
     4141 tcgtcgtcgg caagtgcaca tgaaacgggg aatacgccac cactcaatgc tttacctaaa
     4201 attaatatat ctggtctaac accttcgtgg tcgcaagcca acattttacc ggtacgagcc
     4261 aaacctgttt gtacttcgtc ggcaatgaat aatacattgt gttttttaca caattcgtat
     4321 gattttttca aaaatccact gtcggggaca tatacacctg cctcaccttg aataggttct
     4381 acaagaaatc cagcaacagt tttaccatgt ttttccaata cttcttccaa tgctttggta
     4441 tcgttgtacg gtatgcgaat aaatcccgga gttaaaggac catagtttgc aaatgaatca
     4501 ggatcgttag acatagaaac tatagtaata gtacgaccat gaaaatttcc ttcgcaacaa
     4561 attattttta cgtcatcgtt tgcaattcct tttttcttaa ctccccaacg gcgtgcaagt
     4621 ttcaatgcag tttcatcggc ttcggcaccg gtattcatag gtaatacttt gtcgtatccg
     4681 aaatattttg taacatactc ttcaaattcg cccaatacat tgttgtaaaa tgctcgtgat
     4741 gtaagagtaa gtttttttga ttgttctata agtgcattaa taattttagg atgacagtgt
     4801 ccttggttta ctgcagaata tgccgaaaga aaatcgtaat attttttacc ttctacatcc
     4861 cacatatata caccttcgcc tttttccaac accaccggaa gcggatgata attatgagct
     4921 ccatacttgt cttcgcgtgc catataatct tgaggagtca attgagccat acgctttgtt
     4981 aattttttta aaattagtac taaaaaatga gtgccaaaag tatataatta gttgtgggta
     5041 tgcaattttt ttttgtaaaa aataacatgg atgtgaattt taattttttt ggtaattatt
     5101 gaagctccta aatgtaagta atatataaaa ttaatacttt ttttatattt ttaaattgga
     5161 cagttttttg ggaggttaca caatcaacta aatgatactt ggtcaataga ttttatgcat
     5221 gatgtgct
//
LOCUS       DAOTOW010000050         5187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13326_length_5187_cov_3.450701, whole genome shotgun sequence.
ACCESSION   DAOTOW010000050 DAOTOW010000000
VERSION     DAOTOW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13326_length_5187_cov_3.450701"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="P5243_01430"
     CDS             <1..522
                     /locus_tag="P5243_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18136.1"
                     /translation="ALIQVQVIETPITQTIQLQQGWNLISTYTRPTATTCVNSQGNAA
                     HCISAIVNSNITMIKNADGFWKQGQPNELQSLQYIEQGKGYLIYATTSTNISFTGIPG
                     ISASQYPTTAGWHLIGYPGCTQSNCLSVPISTYFNTTNCQIIKNFDGFWEPNGTVNSI
                     QELLPGKAYYYKK"
     gene            complement(577..1845)
                     /gene="serS"
                     /locus_tag="P5243_01435"
     CDS             complement(577..1845)
                     /gene="serS"
                     /locus_tag="P5243_01435"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRS18137.1"
                     /translation="MLTLQIIREQTEMVLERLAVKHCNANDTIASILSLDDSRKSIQQ
                     ELNAKQAEINALSKEIGQLFQAKKIDEANKAKEQTAVIKEAIKLLEEQMEAVSKQLQD
                     TIISLPNLPHASVPKGRTADDNVVVKTGGIIPDSTGLAPHWELTEKYNLIDFELGNKL
                     TGTGFPVYIGKGARLQRALISFFLDRAIQAGYVEYMPPIVVNEDSGFGTGQLPDKEGQ
                     MYYVNEDNLYLIPTAEVPVTNIFRDTIVKSSDFPIKCTAYTPCFRREAGSYGKDVRGL
                     NRLHQFDKVEIVRIEHPNNSYAALDEMVAHVESLVADLELPYRIVKLCGGDMSFTSAL
                     TYDFEVYSAGQQRWLEVSSVSNFESFQANRLKLRFKDAETGKTVLAHTLNGSALALPR
                     IVAALLENNQTPAGIRIPKILMPYMGCEII"
     gene            complement(1958..2218)
                     /gene="rpmA"
                     /locus_tag="P5243_01440"
     CDS             complement(1958..2218)
                     /gene="rpmA"
                     /locus_tag="P5243_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRS18138.1"
                     /translation="MAHKKGVGSSKNGRDSESKRLGVKLFGGQTAKAGNILVRQRGTV
                     HFAGENVGMGKDHTLFALKDGVVSFRKKTDNKSYVSVIPAEA"
     gene            complement(2235..2546)
                     /gene="rplU"
                     /locus_tag="P5243_01445"
     CDS             complement(2235..2546)
                     /gene="rplU"
                     /locus_tag="P5243_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRS18139.1"
                     /translation="MYAIVEIAGQQFKIAKDDKIFVNRLKTAEGESFDIDSVYLVDND
                     KSVNVGTPLVKGAKVTAKVLSHVKGDKVIIFKKNRRKGYRVKNGFRPSLTQIQIEEIK
                     A"
     gene            2666..3103
                     /locus_tag="P5243_01450"
     CDS             2666..3103
                     /locus_tag="P5243_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18140.1"
                     /translation="MKKFNILIFSLLVFLVSCSESLEEHIVYSHPNNTPAYVEYYAKG
                     DSSKQVVKTIRYYYNGERQEELHLKNGKKNGVCTMWYMNGEKMYEAHYVDDVLHGDFI
                     QWYDNGKKDYEAQYTNGIATGTWKFYNKDGSLKKETHMTPQNK"
     gene            3107..3643
                     /locus_tag="P5243_01455"
     CDS             3107..3643
                     /locus_tag="P5243_01455"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HRS18141.1"
                     /translation="MAIIDEENVLTSIVLDVAKRMVTAAVTAPKAKGIRNMYTAIITG
                     SDIIPIAVKMEQMALEFDVPSFHRDAQNIKQCDAMVLLGTAIQSINLKKCGMCGFANC
                     AEKNKHSTVPCVFNTGDLGIALGSAVSIAMDNRIDNRIMYTVGQAVKELDIFPENVSI
                     IYAIPLKASSKNIFFDRG"
     gene            3714..5153
                     /gene="asnS"
                     /locus_tag="P5243_01460"
     CDS             3714..5153
                     /gene="asnS"
                     /locus_tag="P5243_01460"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRS18142.1"
                     /translation="MRRTEIKTILQKEEIGAEVVVKGWVRNYRGNNFVMFVALNDGSS
                     LQNLQIVIDIEKFDAQLLKKITVGSSLSIQGTIAKSEGKGQSIEVLASSVEVLGTANA
                     DEYPLQPKKHSLEFLREIGHLRFRSNTFGAITRIRHQMIYAIHTFFHERGFYNIHTPI
                     ITGSDCEGAGEMFQVTTLDLKKLPFTKDGNIDFSEDFFGKQTNLTVSGQLEVEMACMA
                     MSKVYTFGPTFRAENSNTSRHLAEFWMIEPEVAFADIYDDMDLAEEFLKYLISYALTN
                     CKEDLEFLQERLLQEEKSKPEQERSMPLIEKLQFVLANDFQRLTYTEAIEILKESKPN
                     KKGQFKYEVKGFGTDLQSEHERYLVEKHFKKPVILTGYPKEIKAFYMKQNPDGKTVAA
                     MDVLFPQIGEIIGGSEREADYDTLYNRMVEMNIPTEELYWYLDSRRYGTVPHAGFGLG
                     FERLMLFVTGMGNIRDVIPFPRYPKSADF"
BASE COUNT         1717 a          814 c          905 g         1751 t
ORIGIN      
        1 gctcttattc aggttcaggt aattgaaaca ccaattactc aaactataca actacaacaa
       61 ggctggaatt taatatcaac gtatactcgt cctacagcta caacttgtgt aaactcgcaa
      121 ggtaatgctg ctcattgtat atcggcaatt gtaaatagta atattacaat gattaaaaat
      181 gcagatgggt tttggaaaca aggacaacct aacgaactcc aatcattaca atatatagaa
      241 caaggtaagg gatatttgat atatgcaact acatctacaa atatatcatt taccggaatt
      301 ccgggtatat cagcatctca atatccaact actgcagggt ggcatttaat tggatatccg
      361 ggatgtactc aaagtaattg tttgtcggtt cccatttcaa cttattttaa tacaacaaat
      421 tgtcaaataa ttaaaaattt tgatggtttt tgggagccga atggtactgt aaattctatt
      481 caggaattat taccgggtaa agcatattat tataaaaagt aacataaaaa aaggctatag
      541 attttctata gcctttttta taatggttac taaaaattat attatttcac atcccatata
      601 tggcattaat attttaggta ttctaatacc ggcaggagtt tgattatttt caagcaatgc
      661 tgctactata cgaggtaaag ccaatgcact accgttaaga gtatgtgcta acactgtttt
      721 tcctgtttca gcatctttaa atctgagttt aagacgattt gcttgaaacg attcaaaatt
      781 agatacagaa ctaacttcta accagcgttg ttgccctgca gaatacactt caaaatcata
      841 ggtaagtgca gaggtaaagc tcatatcgcc tccacataat tttacaatac gatagggtaa
      901 ttctaagtct gcaaccaatg attctacgtg tgctaccatt tcatctaatg cagcatagga
      961 gttgtttgga tgttctatgc gaacaatttc aactttgtcg aattggtgca agcggttgag
     1021 tccacgcacg tctttaccgt acgagccggc ttcgcgacgg aaacatgggg tatatgcagt
     1081 acattttatt gggaaatcgc tcgatttgac aatggtatct ctaaatatgt tggttaccgg
     1141 aacttctgct gttggtatga ggtacaaatt atcttcgttt acataataca tttgcccttc
     1201 tttatcgggt agttgacctg ttccaaaacc ggaatcctcg tttaccacta ttggtggcat
     1261 atattctacg tatcctgctt gtatagcacg gtcaagaaaa aatgatatga gagcacgttg
     1321 taatcgagct cctttaccta tataaacagg aaatcctgtt cctgtgagtt tatttcctaa
     1381 ttcaaaatct atgagattat atttttcggt taattcccag tgaggtgcca aaccggtact
     1441 gtcgggaata atacctcctg ttttaactac aacattatca tcagcagttc gtcctttggg
     1501 tacacttgca tgcggtaaat ttggaagtga aataattgta tcttgcagtt gtttacttac
     1561 tgcctccatt tgttcttcca acagtttaat agcttctttt attactgcag tctgttcttt
     1621 tgctttatta gcctcatcta ttttttttgc ctgaaataat tggccaattt cttttgaaag
     1681 agcatttatt tcagcttgtt tagcattcaa ttcttgctgt atagatttac gagaatcatc
     1741 gagcgataaa attgaggcta tagtatcgtt agcattgcag tgttttacag ctaaacgctc
     1801 aagaaccatt tcggtttgtt ctctaataat ctgaagggtt aacatatggt tttataattt
     1861 aaagtaaact acaattatat aaacaaaaaa actcctgagt atagtatccg catgaggata
     1921 tataatcaag agtatatatt ttaataaata ctgataatta tgcctctgca ggaattacag
     1981 aaacatagga tttattatcg gttttttttc tgaatgatac aacaccgtct ttaagagcaa
     2041 ataaagtatg gtctttaccc attcctacgt tttcacctgc aaaatgaaca gttccacgtt
     2101 gtcttacaag aatatttcct gcttttgcag tttgtccgcc aaaaagtttc acgcctaatc
     2161 gtttactttc tgaatctcta ccgttttttg aactaccaac cccttttttg tgtgccatgg
     2221 ttcctaagtt ttaattatgc tttaatttct tctatttgaa tttgagttaa acttggtctg
     2281 aaaccatttt ttactctata tccttttctg cggttttttt tgaaaatgat aactttatct
     2341 cctttaacat gagaaagtac ttttgcagta acttttgctc cttttacaag aggagtacct
     2401 acatttacac ttttatcatt atctactaaa tataccgagt caatatcaaa tgattcgcct
     2461 tcggctgttt taagacgatt aacaaagatt ttgtcgtctt tagcaatttt aaattgctgt
     2521 cctgctattt ctactattgc gtacattatt atattttatt atatcctccg aaaaatggac
     2581 tgcaaatgta taaaacttta tataattgac aaatactttt taaaattaat tatacatttg
     2641 tacaaatatt tttacaattt tgtatatgaa aaaatttaat atccttattt tcagcttgtt
     2701 agtttttttg gtttcatgtt ctgaatcgtt agaagaacat atagtatatt cacatccaaa
     2761 caatactccg gcgtatgtag aatattatgc caaaggtgat tccagtaaac aagtagtaaa
     2821 aacaattcgg tattactata atggtgaacg acaagaagaa ttacatttaa aaaatggtaa
     2881 aaaaaatggt gtgtgtacca tgtggtatat gaatggcgaa aaaatgtatg aagctcatta
     2941 tgttgacgat gtattgcatg gtgattttat acaatggtat gataatggaa aaaaagatta
     3001 tgaagctcaa tatacaaatg gtattgcaac aggaacttgg aaattttata acaaagatgg
     3061 ttcccttaaa aaagaaacac atatgactcc tcaaaataaa taacatatgg caataattga
     3121 tgaagaaaat gttttgacat ctattgtatt agatgttgct aagcgaatgg taacggctgc
     3181 tgttacggca cccaaagcaa aaggaatacg taatatgtat actgcaataa ttaccggtag
     3241 cgacataata cctatagctg taaaaatgga acaaatggca ttagaatttg atgtgccatc
     3301 gtttcataga gatgctcaaa atataaaaca atgtgatgca atggtattat tgggaaccgc
     3361 aatacaatct ataaatctta aaaaatgtgg tatgtgcgga tttgcaaatt gtgccgagaa
     3421 aaataaacat tctacagtac catgtgtgtt caataccggt gatttgggta tagctttagg
     3481 ttctgctgtt agtattgcta tggacaatag aatagataat agaataatgt atactgttgg
     3541 gcaagctgta aaagaacttg atatttttcc tgaaaatgtt tcaattattt atgctattcc
     3601 tcttaaagca agtagtaaaa atattttttt tgatagagga taattataat tcattttttt
     3661 gtgtacaatc acaaatcgtg taaatttgtc gaaaaacata agaacatttt aatatgcgta
     3721 gaacagaaat aaaaaccata ctccaaaaag aagaaattgg tgcagaagta gtagtaaaag
     3781 ggtgggtgcg taattaccgg ggcaataatt ttgtaatgtt tgttgctctt aatgatggtt
     3841 catcgcttca gaatttgcaa atagtaatag atattgaaaa atttgatgca caactattaa
     3901 aaaaaattac cgttggctct tcactttcta ttcaaggaac tattgctaaa tcagaaggta
     3961 aaggtcagtc tattgaagta ttggcaagtt ctgttgaggt tttgggtact gcaaatgccg
     4021 atgagtatcc attacaacca aaaaaacatt cacttgaatt tcttcgcgaa attgggcatt
     4081 tgcgttttcg cagtaataca tttggagcta ttacacgcat tcgtcatcaa atgatttatg
     4141 ctattcatac attttttcac gaacgaggat tttataatat tcatactcct attataactg
     4201 gttctgactg tgaaggagct ggtgaaatgt ttcaagtaac aacattagat cttaaaaaat
     4261 taccttttac aaaagatggg aatatagatt tttctgaaga tttctttgga aaacaaacaa
     4321 atttaacggt ttcgggacaa ttagaagtag aaatggcgtg tatggcaatg tctaaagtat
     4381 atacatttgg acctactttt agagcagaaa attcaaatac atcgcgtcat ttagcagaat
     4441 tttggatgat agaacccgaa gttgcttttg ccgatatata tgatgatatg gatttagccg
     4501 aggagttttt gaaatatctt atttcatacg ctcttaccaa ttgtaaagaa gatttggaat
     4561 ttttgcaaga aagattattg caagaagaaa aatcaaaacc tgagcaagaa cgttcaatgc
     4621 ctcttataga aaaattgcaa tttgttttgg caaatgattt tcaaagactt acctataccg
     4681 aagcaattga aattttgaaa gaatcaaaac caaataaaaa agggcaattt aaatatgaag
     4741 taaaaggttt tggtaccgat ttgcaaagtg aacacgaacg atatttagta gaaaagcatt
     4801 ttaaaaaacc ggtaattctt accggatatc caaaagagat taaagccttt tatatgaaac
     4861 aaaatcccga tggtaaaaca gtagctgcaa tggatgtttt gtttccacaa attggagaaa
     4921 taattggagg tagcgaacgt gaagcagatt atgatacatt atataaccgt atggttgaga
     4981 tgaatattcc taccgaagag ttatattggt atcttgattc gcgtagatat ggaactgtac
     5041 ctcacgccgg ttttgggctt ggatttgaac gtttaatgtt gtttgtaacc ggtatgggaa
     5101 atattcgtga tgttatacca tttccacggt atcctaagag tgctgatttt taattgatac
     5161 aagatgtcta aaccgcttca aatagta
//
LOCUS       DAOTOW010000051         5179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13345_length_5179_cov_3.298400, whole genome shotgun sequence.
ACCESSION   DAOTOW010000051 DAOTOW010000000
VERSION     DAOTOW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13345_length_5179_cov_3.298400"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1170
                     /gene="pdxA"
                     /locus_tag="P5243_01465"
     CDS             142..1170
                     /gene="pdxA"
                     /locus_tag="P5243_01465"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRS18143.1"
                     /translation="MRHIRVGITHGDINGIGYEVIMKALSDNRVTEKITVIVYGSPKA
                     AAFHRKAIDLQNFSFNLIQTAQEANSKRPNIIDCCDPELKVEPGISTIDAGKAAFQAL
                     KAAVADLRNNTIDVVVTAPINKKNINEAGFEFPGHTEFFASQFNSPNHVMLLMNDIMK
                     VGVVAGHIPVSQISSFITKEKIVQKLEVLNSSMISDFGIRKPRIAVLGLNPHAGDEGL
                     LGNEEQTVIIPAIQEARNKGIMALGPYAADGFFGSGEFSKFDVILGMYHDQVLAPFKA
                     LAFDNAVNVTLGLPIVRVSPAHGTAYSLVGQNQASELSMIQAIFTACDIYESRVNYRE
                     LVANKLTQ"
     gene            1177..1758
                     /gene="ruvA"
                     /locus_tag="P5243_01470"
     CDS             1177..1758
                     /gene="ruvA"
                     /locus_tag="P5243_01470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRS18144.1"
                     /translation="MLEYISGTIQKLTPTYAVIDNAGIGYFVNISVYTCQKITQNSQT
                     TLLVHEIIREDAHELFGFFDETERSMFRMLISVSGVGANTARVILSKLSAQEVASSIA
                     LGNVQVLQAVKGIGGKTAQRIIVDLKDKVTSISQGEEIFVSQGNTLVHEALSALLMLG
                     FAKNSVEKIVQTIVSEQTYTSVEDVVKAALKRL"
     gene            1794..>2944
                     /gene="sprA"
                     /locus_tag="P5243_01475"
     CDS             1794..>2944
                     /gene="sprA"
                     /locus_tag="P5243_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRS18145.1"
                     /translation="MKNLHKAYFILVFAMIGIVVASASVYMYHTSSYLSYKSVAPPDT
                     IPKQVDLKYPLPQENPNPFKDNVLQSSGIRLKESPEYTYKVTFNEETGEYEIVKMLGD
                     IPIGAPYSMSAKEYFDFSASQRRDQYWRTRAYESRGGGSSSYKPSLDVPGANNETFFD
                     ENFVDIKPQGAAEISFGFKHTNNQNPRFSKKQQRKTQFDFDNKIQMSVTGKVGDKVAL
                     TVKYDTESQFEFDKEVKLEYEGDEDEIIKKINVGDVSFPIPSSLITGSQSLFGVHTEM
                     QFGKLYVNSVFSRQQGEFSTIKVQGGAQVEEFKIRADNYDKNKHFFLGHYFRDNYEQS
                     FATIPEISSGIQIRKVEIWVTQTSVGKEDARNILAVTDLGDEYVLGPDG"
     assembly_gap    2945..2954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2955..>5179
                     /gene="sprA"
                     /locus_tag="P5243_01480"
     CDS             <2955..>5179
                     /gene="sprA"
                     /locus_tag="P5243_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRS18146.1"
                     /translation="YSLPSNGKLYKELKNIQGIRNINNISSLLTGKLEQGRDYEKIEQ
                     AVLLKPNEYDINNKLGYISLRSAINPAKILAVAYEYEYQGQVYQVGEFTSDGVEHPDV
                     LIVKMLKGSNMNPTYNNWDLMMKNVYALGGYKITNEDFSLNILYEDDRTGTPIPYIPE
                     GNIKGKNLLSVMNLDTITNDKLPYADGIFDFVEGYTINSSRGLLFFPVLEPFGSHLQK
                     KIGNPTVAQKYVYTQLYDSTQSAARQVAEKNKFVIQGKYKSAVRSEIPLNSTNVEKNS
                     VTVIAGGRVLVEGVDYTVDYMGGTVKIINEGLLQSGADITIKAESNPLFSMKTKNLMG
                     TRFDYRYNQNINFGATIMRLSEKPAVQKVGFDDYPISNTIWGVDANYSTKSIFLTSLV
                     DKYVPFVKTKEVSQINLSGEFAQLIPGQSKYISDAVELDNFESTEGRIYLREPTAWKI
                     ASTPQLQKNMFPEGYLINDLAYNYNKAHISWYNINTTFYQTRNSPVSKQIQEQNFSRA
                     VYQTSLFPNRDLTSVEDMPMTILNIAYYPYERGQNNYDTKGRPGISAGINSQGYLKNP
                     ETRWGGIMQSLTTTDFEESNVEYIEFWLMDPFVDDTAAIHKGGDFYINLGRISEDVLR
                     DGRKSAENGMLPDPTRYDQTIWGRVPNYSIVETGFSNDVERIIQDVGLDGLNDDAERK
                     FFANYLKEIKDIVSDESIFQRFYNDPSADNYSSYLDNRGVNSDIVWMYKYFSGNDGNT
                     P"
BASE COUNT         1846 a          723 c          980 g         1620 t
ORIGIN      
        1 taatataaaa gtcttggaat tatgcttaaa aattttatat ttgctcggta aaaagtatcc
       61 attttataat aaatggtaat ttttatctaa aacttttatt tacaatatgt tttagaaatt
      121 caaatttata gcaaatatac tatgagacat atacgagttg gaataacaca tggcgatata
      181 aacggtattg gatatgaagt aataatgaag gctctaagtg ataatagagt tacagaaaaa
      241 attaccgtta tagtatatgg ttcgccaaaa gctgcagctt ttcatcgtaa agcgattgac
      301 ttgcagaatt ttagttttaa tcttatacaa acagcccaag aggctaattc caaacgacca
      361 aatataatag attgttgcga tccggaatta aaagtagaac cgggtatttc tacaatagat
      421 gcaggcaagg ctgcttttca agcattaaaa gcagcagtag ccgatttgcg aaataatact
      481 attgatgttg ttgttacagc accaataaat aaaaagaata ttaacgaagc agggtttgaa
      541 tttccgggac atactgaatt ttttgcttct cagtttaatt caccaaatca tgtaatgttg
      601 cttatgaatg atataatgaa agtaggtgta gttgcaggtc atataccggt ttcacaaata
      661 tcttcgttta ttacaaaaga aaaaattgtt caaaaattag aggtgttaaa ttcatctatg
      721 atttcagatt ttggaattcg aaagcctcgt attgctgttt tgggattgaa tcctcatgcc
      781 ggagatgaag gattactagg aaatgaagaa caaacagtaa taatacctgc aattcaagaa
      841 gctcgtaata agggtattat ggctcttggg ccatatgcgg ccgatggttt ttttggctcc
      901 ggtgaatttt caaaatttga cgttattttg ggaatgtatc atgaccaagt attagcacca
      961 ttcaaggcgt tagcatttga taatgccgta aatgtaactc tagggctccc tattgtgaga
     1021 gtgtcgccgg cacatggtac tgcatatagt ctggttggtc aaaatcaagc ttctgaatta
     1081 tcaatgattc aggcaatttt tacggcttgc gatatttatg aatcgcgagt aaattataga
     1141 gaattagttg ctaataaact tacacaatag aatcgtatgt tagaatacat tagcggtaca
     1201 atacaaaaac taactcctac ctatgcagtg atagataatg caggtattgg gtattttgtt
     1261 aatataagtg tatatacatg tcaaaaaatc acacaaaatt ctcaaacaac tttgttggta
     1321 catgaaataa tacgtgaaga tgcccatgaa ttatttggtt tttttgatga gactgaacgt
     1381 tcaatgtttc gcatgctaat ttccgtttca ggagtaggag ctaatactgc acgagtaata
     1441 ttatcaaagt tgtcggcaca agaagtagcg tcatcaatag cattaggcaa tgtgcaagta
     1501 ttacaagcag taaaaggtat tggaggaaaa acagctcagc gtataatagt tgatttaaaa
     1561 gataaggtta catcaatttc tcagggtgaa gaaatttttg tatctcaagg caatacttta
     1621 gttcacgaag cgttatcagc actgcttatg ttgggatttg ccaaaaattc agttgagaaa
     1681 atagtgcaga caatagtatc ggaacaaaca tatacaagtg tagaagacgt agtaaaagct
     1741 gcattaaaac gtttgtaata ctttggaaat gccataaaat aatatagaga agtttgaaga
     1801 atttacacaa agcatatttc atattagtat ttgctatgat aggcatagtg gttgcctcag
     1861 catctgtgta tatgtatcat acttcttcgt atttgtcata taaatctgta gccccacccg
     1921 atactattcc taaacaggtt gatttaaagt atcctctccc gcaagaaaat ccaaatccgt
     1981 ttaaagataa tgtgctgcaa agttcaggaa tacgacttaa agaatctcct gaatatacgt
     2041 ataaagtaac gtttaatgaa gaaacaggtg agtatgaaat tgtaaaaatg cttggagata
     2101 ttcctattgg cgctccgtat tctatgtctg ctaaagaata ttttgatttt tctgcatcac
     2161 aacgacgaga ccaatattgg agaaccagag catatgagtc aaggggagga ggtagttctt
     2221 cgtacaaacc aagtttagat gtgccgggag ctaacaatga aacatttttt gatgaaaatt
     2281 ttgttgatat taaacctcag ggagctgctg aaatttcatt tggttttaaa catacaaaca
     2341 atcaaaatcc tcgattttct aaaaagcagc aacgaaaaac gcaatttgat tttgataata
     2401 aaattcaaat gagtgtaacg ggtaaagttg gtgataaagt tgctcttaca gtaaaatatg
     2461 ataccgaatc tcaatttgaa tttgataaag aagtgaaact tgaatatgaa ggagatgaag
     2521 atgaaataat taaaaaaata aacgtaggtg atgtttcatt tccaatacca agttctctta
     2581 ttacaggtag tcaaagttta tttggggtac ataccgaaat gcaatttggt aaattatatg
     2641 tcaatagtgt tttttctcgt caacaaggag aattttctac aattaaagtc caggggggag
     2701 ctcaggtaga agaatttaaa attcgtgccg ataattatga taaaaataaa cactttttct
     2761 tagggcacta ttttagagat aattacgaac aatcttttgc aacaattccc gaaatttcca
     2821 gtggtattca aattcgtaaa gttgaaattt gggtaactca aacttctgta ggaaaagaag
     2881 atgctcgaaa tatattagca gttacagatt taggcgatga atatgtattg gggcccgatg
     2941 gtaannnnnn nnnnatattc gttgccttct aatggtaaat tgtataaaga attaaagaat
     3001 attcaaggaa ttcgtaatat aaataatatt tcatcgcttt taacaggaaa acttgaacaa
     3061 ggtcgtgact atgaaaaaat agaacaagct gttttgttga agccaaatga atatgatata
     3121 aataataaat tagggtatat atcattacgt tcagcaataa atcctgcaaa aatacttgca
     3181 gtagcatatg aatacgaata tcagggacaa gtgtatcagg taggtgaatt taccagcgat
     3241 ggtgtagaac accctgatgt attaattgtt aaaatgttaa aaggttctaa tatgaatccc
     3301 acttataata attgggattt aatgatgaaa aacgtatatg cccttggtgg atataaaatt
     3361 acaaatgaag attttagcct taatatattg tatgaagatg atagaaccgg aacgccaatt
     3421 ccctatatac ctgaaggaaa tataaaaggg aaaaacttat tatcggttat gaacttagat
     3481 acaattacaa atgataaatt gccatatgcc gatggaatat ttgattttgt agaagggtac
     3541 accataaatt cttcaagagg attgttgttt tttcctgtct tagagccttt tggcagtcat
     3601 ttgcaaaaaa aaattggaaa tcctacagtt gctcaaaaat atgtatatac tcaattatat
     3661 gattcaacac aaagtgctgc acgccaagta gcagaaaaaa ataagtttgt aattcaagga
     3721 aaatataagt cggctgtacg ttccgaaata ccattaaatt caacaaatgt agaaaaaaat
     3781 tcggtaactg taattgcagg tggccgtgtg ctggttgaag gagttgacta tactgttgat
     3841 tatatgggag gaactgtaaa aattattaac gaaggtttgt tgcaatcggg tgccgatatt
     3901 accataaaag cagaaagtaa tccattgttt agtatgaaaa ctaaaaattt aatgggaacc
     3961 cgatttgatt atagatacaa tcaaaatata aattttggtg ctactatcat gcgtttgtca
     4021 gaaaaacctg cagttcaaaa ggttggtttt gatgattatc caatatctaa cactatttgg
     4081 ggggtcgatg caaattattc tacaaaatct atttttctta cctcgttagt tgataaatat
     4141 gtgccatttg taaaaacgaa agaagtttct caaattaatt tgtccggcga atttgctcaa
     4201 ttaattccgg gacaatcaaa atatatttca gatgccgtag aacttgataa ttttgaaagt
     4261 acagaaggga gaatttattt gcgtgaacca actgcgtgga aaatagcaag tacaccgcaa
     4321 ttgcaaaaaa atatgtttcc cgaaggatat ttaattaacg atttggcata taattataat
     4381 aaagctcata tttcatggta taacataaat actacgtttt atcaaacacg taattctccc
     4441 gtaagtaaac aaattcaaga acaaaatttt tcgcgagctg tatatcaaac tagtttattt
     4501 cctaatcgag atttaacttc ggttgaagat atgcctatga caatattaaa tatagcatat
     4561 tatccgtatg aacgaggaca aaataattac gatactaaag gaagaccggg aatttctgcc
     4621 ggaataaatt ctcaaggata tttaaaaaat cctgaaactc gatggggagg gattatgcaa
     4681 tcgcttacta ctactgattt tgaagagagt aatgtagaat atatagaatt ttggcttatg
     4741 gacccttttg tagatgatac agcagcaatt cataaggggg gcgattttta tattaatcta
     4801 ggtagaattt ctgaagatgt tttgcgagat ggtcgtaaat ctgccgaaaa tggaatgcta
     4861 ccagatccaa cacgatatga tcaaacaata tggggtagag tacctaatta ttctattgta
     4921 gaaacaggtt ttagtaatga tgtggaacgg attatacaag acgttggatt agatggattg
     4981 aatgatgatg ctgaacgaaa attttttgca aattatttga aagaaattaa agatattgta
     5041 agcgatgaat caatttttca acgcttttac aatgaccctt ctgccgataa ttattcatcg
     5101 tatcttgata atagaggggt aaattctgat atagtttgga tgtataaata ttttagcgga
     5161 aatgatggta atactccta
//
LOCUS       DAOTOW010000052         5176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13358_length_5176_cov_3.227495, whole genome shotgun sequence.
ACCESSION   DAOTOW010000052 DAOTOW010000000
VERSION     DAOTOW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13358_length_5176_cov_3.227495"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2129)
                     /locus_tag="P5243_01485"
     CDS             complement(<1..2129)
                     /locus_tag="P5243_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRS18147.1"
                     /translation="MQESTSFFTRIFADDRIRIVSGIVSVSIAIFFLLSFVSFIFSWQ
                     NDQSELLHQSSWEFLFSSDQKLEDPAIANSYTSISEVEPQVFEIQNWGGRLGALVSKK
                     IIHDWFGVSSFLLVYLCVILGLYLFKIRIMSIRSQVFILLLSVLVGSLTLGYIFNDAW
                     DANFGGFFGYYVSYYWLGNLVGKVTAGLIVLTIFTIYLLFTFKNSLSFMKGVFKTSNN
                     EEHTVDIAFDDTTIEETLYSEGIDNQLDETVLTNVDEKMPDNIEQQEIVLEENTIVDE
                     QETEFISHAVFEVKQSDDNQEQEIEDLEETEDEHHIYDNKVELEVEDMDITNTMDNND
                     EVEFISTELQDYDPTLDLSHYTLPPIDLLENHQTSNNEVSSQELADNKNKIEQTLGNY
                     GIQIDKISATIGPTITLYEIVPAAGVRISKIKNLEDDIALSLSALGIRIIAPIPGKGT
                     IGIEVPNKNPEVVSMRSIIASKAFQEAKMDLPVALGKTISNETYVFDLTKMPHLLVAG
                     ATGQGKSVGLNAIMTSLLYKKHPAQLKFVLVDPKKVELTLYQNIEKHFLAKLPDEEEA
                     IITDTQKVVNTLNSLCIEMDNRYDLLKEAKLRNIKEYNERFIARRLNPEKGHRYLPYI
                     VVVIDEFADLIITAGKEVEIPLARLAQLARAIGIHLIIATQRPAANIITGAIKANFPA
                     RLAFRVASGIDSRTILDATGANQLIGKG"
     gene            complement(2140..2784)
                     /locus_tag="P5243_01490"
     CDS             complement(2140..2784)
                     /locus_tag="P5243_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18148.1"
                     /translation="MNLLHACSIAGENEGIIEYDIEFNEEERKKNEIIDLLPKTMKYY
                     FKNKSAVSEISYMGVFRTAYISNFQDKTNTILFYFMPNKYYCTTNFGEKTIGYDPMPG
                     IQLEETGETKDIKGIKAYRVRVTFEDKSQEPYDVWYTKDFKVHNPNWHTPYKEINGVL
                     LDYRIKMKGISMHMTLNKFSEEVVDTAKFQIQKSYKKVSPKEMDEIFDKHLNMF"
     gene            complement(2965..4014)
                     /locus_tag="P5243_01495"
     CDS             complement(2965..4014)
                     /locus_tag="P5243_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HRS18149.1"
                     /translation="MNIQFSQTIIEKIVIHRIGNKNENQGIELSHSEVEIHDEHTKNI
                     LLSFFLTPFKAPDLYQFNLETISDEDSVLQSVTRMFDFEQEFYVQSANIAQKLYAVSN
                     HPNIKQGELYVVYFRNCEVDDNLVDAIGIFKSENKDTYIKVFQDGENFGVEHERGINV
                     KKLDKGSIIFNVDSETGYVLKVLDNTNKQNEALYWIDDFLAAKKIENDYFNTATFLQI
                     CKNFSENILTEKNNVQKNEQLGFMNKSYEYFKENDYFDEVDFKKTVIANPEIIESFDM
                     FKQNYEESYEIEPKTEFDISQEAVKKSKKFVRSVIKLDKNFHIYVHSKPEYIEKGFDS
                     NRKLNFYKVFFEKES"
     gene            complement(4027..4485)
                     /locus_tag="P5243_01500"
     CDS             complement(4027..4485)
                     /locus_tag="P5243_01500"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRS18150.1"
                     /translation="MELYSGFITSPCGIIEIRANQNAVFSIQFVDTVTIANENFVISQ
                     SKQELQEYFNGTRTEFTMPIQYIKSIYSTKVYEACMQIPYGTTTTYKNIAYTISSPQS
                     VRAIGNVLHNNPILIVIPCHRIVHASHSKQGYKGGIWRKQLLLDLEKKYI"
     gene            complement(4485..5093)
                     /locus_tag="P5243_01505"
     CDS             complement(4485..5093)
                     /locus_tag="P5243_01505"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRS18151.1"
                     /translation="MKKEYWAVLLVFLILFVDQLTKILVKTSMHAEEMIPILGDRVFI
                     YFVENPGFAFGMKFGGVIGKIALTLFRIVASVFIILYIRKLIKRDAPLDVIVGLSAIL
                     AGAMGNIIDSALYGILFSESTFGTVAQFMPESGGYAPFLQGHVVDMIYCPIIRGTFPE
                     WLPVWGGEPFTFFKPIFNIADSAITVGVLYLLLFQRKFFKEI"
BASE COUNT         1736 a          893 c          642 g         1905 t
ORIGIN      
        1 cctttaccta taagttgatt tgctcctgta gcatctaaaa ttgtacgaga atcaataccg
       61 gaagctactc taaaagctaa acgagccgga aaatttgctt taattgcacc tgtaatgata
      121 ttagcggcgg gacgctgagt tgcaattata agatgaatac caatagcacg tgctaattgc
      181 gctaaacgag caagtggtat ttctacctct ttacctgctg taataattaa atcggcaaat
      241 tcatcaatta caacaacaat atagggtaaa tatctatgac ctttttcggg attgagccta
      301 cgtgcaataa atcgctcatt atattctttt atatttcgta atttagcttc tttaagcaaa
      361 tcataccgat tatccatttc tatgcacaat gaattaagcg tatttacaac cttttgtgta
      421 tcggttatta tagcctcttc ttcgtcgggt aattttgcaa gaaagtgctt ttctatattt
      481 tgatacagcg ttaactctac ttttttaggg tcaaccaata cgaattttaa ttgagccggg
      541 tgttttttat acaaaagtga agtcattata gcatttaacc caaccgattt accttgtccg
      601 gttgccccag caaccaataa atgaggcatt ttggttaaat caaatacata cgtttcgtta
      661 gaaatagttt tacctaaagc taccggtaaa tccatttttg cctcctgaaa agcttttgat
      721 gctataatag aacgcattga aaccacctct ggatttttat ttggtacttc tataccaatt
      781 gttccttttc ccggaatagg agcaattatt ctaattccaa gagcagataa actcaatgca
      841 atatcatctt ctaaattttt aatttttgat atacgaacac ctgcagcagg cacaatttca
      901 tacaatgtaa tagttgggcc aatagtggca gaaattttat ctatttgtat tccataatta
      961 cctaaagttt gttctatctt atttttatta tctgccaatt cttgcgacga cacttcatta
     1021 tttgacgttt gatgattttc taacaaatct attggtggca atgtatagtg cgataaatcc
     1081 aaagtaggat cataatcttg taattcggta gaaataaatt caacttcatc attattatcc
     1141 atggtatttg ttatatccat gtcttctacc tctaactcta ctttattatc atatatatgg
     1201 tgttcatctt ctgtttcttc taaatcttct atttcttgtt cttgattatc atcagattgt
     1261 ttaacttcaa atactgcatg tgaaatgaat tctgtttctt gttcatctac tatagtattt
     1321 tcttctaaaa ctatttcttg ctgttcaata ttatcaggca ttttttcatc aacatttgtt
     1381 agtacagttt cgtctaattg attatctatg ccttctgaat acaatgtttc ttctattgta
     1441 gtatcatcaa atgctatatc tactgtatgt tcttcattgt ttgatgtttt aaacacacct
     1501 ttcataaacg ataaagaatt cttaaatgta aaaagcagat atattgtaaa tattgtaaga
     1561 acaattaacc ccgcagttac tttaccaact aagttaccta accaatagta acttacataa
     1621 tatccaaaga atccaccaaa attagcatcc caagcatcgt taaatatata tccgagtgta
     1681 agcgacccta ccaataccga aagtaataaa ataaacactt gagagcgaat actcataata
     1741 cgtattttaa acaaatacaa accaagtatt acacacaagt acactaaaag aaacgacgat
     1801 actccaaacc aatcatgtat aatttttttt gatactaaag cacctaatct acctccccaa
     1861 ttttgtattt caaacacttg gggttcaact tcagaaatac ttgtatatga attagctata
     1921 gccggatctt ctaatttttg atctgaagaa aataaaaatt cccatgaaga ctgatgcagt
     1981 aattcactct gatcgttctg ccatgaaaat ataaatgata caaatgaaag caagaaaaaa
     2041 atagcaatgg atacagacac aattccactc acgattctta ttctatcatc ggcaaaaatg
     2101 cgagtaaaaa aggaagttga ttcttgcata aaattctaat taaaacatat ttaagtgttt
     2161 atcaaaaatt tcatccattt cctttggact tactttttta tatgattttt gaatttgaaa
     2221 ttttgcagta tcaacaactt cttccgagaa tttatttaat gtcatatgca ttgatatgcc
     2281 tttcatttta attctgtaat ctaacaaaac tccgtttatc tctttatatg gagtatgcca
     2341 attgggattg tgtactttaa aatctttggt ataccataca tcatacggtt cttgagattt
     2401 atcttcaaag gtaaccctta ctctatatgc tttaattcct tttatatctt ttgtttctcc
     2461 tgtttcttca agttgaattc cgggcatagg gtcatatcca attgtttttt cgccaaaatt
     2521 tgtagtacaa tagtatttat ttggcatgaa gtaaaataaa atagtatttg ttttatcttg
     2581 aaaattagaa atatatgctg ttctaaaaac acccatataa ctaatttcac ttacagcact
     2641 tttattcttg aaataatatt tcattgtttt aggcaataaa tctattattt cattcttttt
     2701 gcgttcttct tcattaaatt ctatatcata ttctataatt ccttcatttt cacctgcaat
     2761 agaacacgca tgcaataaat tcaaaatgta tataaaaata catactttaa tacagattga
     2821 actaatactt ctttgaaatt gcatatcgaa atttctacct tttatttata aacttgagca
     2881 aaagtaatat ttttttttgt agcattagta gaaaaacgag ctattattaa taacttttaa
     2941 actaaactta caaacaatat atttttaaga ttctttttca aaaaatactt tgtaaaaatt
     3001 caattttcta tttgaatcaa atcctttttc aatatactct ggtttagaat gaacatatat
     3061 atgaaaattc ttatctaatt taattacact tcttacaaat ttcttagatt ttttaacagc
     3121 ttcttgtgaa atatcaaatt ctgttttggg ttcaatttca tatgattctt catagttttg
     3181 tttaaacata tcaaaacttt caattatttc aggatttgca attaccgttt ttttaaagtc
     3241 aacctcatca aaataatcgt tttctttgaa atattcgtaa gatttattca tgaatcctag
     3301 ttgttcattt ttttgaacat tatttttctc tgttaatata ttctcactaa aatttttaca
     3361 aatctgtaaa aaagtagcag tgttgaaata atcattttct atttttttgg cagccaaaaa
     3421 atcatctatc caatatagtg cttcattttg tttattggtg ttatccaaca ctttaagcac
     3481 ataccctgtt tctgaatcaa cattaaatat tatacttcct ttgtctaatt ttttgacatt
     3541 aattccccgt tcgtgttcta caccaaagtt ttcaccatct tgaaaaactt tgatatatgt
     3601 atctttattt tctgatttaa aaattcctat tgcatctact aaattgtcat caacttcaca
     3661 atttctaaaa taaaccacat ataattctcc ttgttttata ttaggatgat ttgaaacagc
     3721 atataatttt tgagcaatat ttgcagattg cacataaaac tcctgttcaa aatcaaacat
     3781 tcttgtaaca ctctgcaaaa cagaatcttc atctgatata gtttcaagat tgaactgata
     3841 taaatcggga gctttaaatg gtgttaaaaa aaatgacaat aaaatatttt ttgtatgttc
     3901 atcatgaatt tcaacttctg aatgtgacaa ttcaattcct tgattttcgt ttttattgcc
     3961 aattctgtga atgacaattt tttctattat agtctgtgaa aattgtatat tcatatgtag
     4021 tttaatttat atatactttt tttctaaatc caacaacaat tgttttctcc aaataccacc
     4081 tttgtaccct tgctttgaat gagaagcatg tacaattcta tgacaaggaa taactataag
     4141 aattggattg ttatgcaata catttccaat agctcttacg gattgtggcg atgatatagt
     4201 atatgctata tttttatatg tagttgttgt tccgtaagga atctgcatac acgcttcata
     4261 tactttggtt gaataaatag attttatata ctgaatcggc attgtaaact cagtacgtgt
     4321 cccattaaaa tattcctgta attcttgttt gctttgacta attacaaaat tttcatttgc
     4381 aatggtaact gtatcaacaa attgaattga aaaaacagca ttttgatttg ctcgtatttc
     4441 aatgatacca caaggagaag taatgaaacc cgaatacaat tccattatat ttctttaaaa
     4501 aattttcttt gaaacaacaa taaatataaa accccaactg taatagcaga atcggctata
     4561 ttaaaaatag gtttaaaaaa tgtaaatggt tctcctcccc atacaggaag ccattcagga
     4621 aacgtacctc gtataattgg acaataaatc atatcaacca catgcccttg caaaaaagga
     4681 gcatatccac cggactcagg cataaattga gctactgttc caaatgtact ttcgctaaaa
     4741 agtattccat acaaagcact gtctattata ttacccatag caccggcaag aattgcactt
     4801 aaaccaacaa ttacatcaag aggagcatct cgtttaatta atttccgtat gtacaatatt
     4861 ataaaaacag atgcaacaat tctaaacaat gtaagagcaa ttttaccaat tactccaccg
     4921 aatttcattc caaatgcaaa accgggattt tcaacaaaat aaataaaaac tctatcacct
     4981 aatataggta tcatttcttc ggcatgcatg cttgttttta ctaatatttt ggttaattgg
     5041 tcaacaaaca aaataagaaa aacaagcaaa acagcccaat attctttttt catacaaatg
     5101 atatcaataa caatttattt agaacccgca ttttttgcat caatactaag tgtagcatga
     5161 ggcactgcac gtaatc
//
LOCUS       DAOTOW010000053         5172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13368_length_5172_cov_2.735392, whole genome shotgun sequence.
ACCESSION   DAOTOW010000053 DAOTOW010000000
VERSION     DAOTOW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13368_length_5172_cov_2.735392"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1527)
                     /locus_tag="P5243_01510"
     CDS             complement(58..1527)
                     /locus_tag="P5243_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRS18152.1"
                     /translation="MDITSQNFLLIGSLLLFISLIAGKTSYKFGIPVVVLFISIGMLA
                     GSEGIGGIYFDNPHVAQFIGIIALNFILFSGGLDTDYASIRPVLGQGTLLSTLGVLIT
                     AGTFGTFVWFITDFSIYESLLLGSIVSSTDAAAVFSILRSKNIALKNNIRPTLELESG
                     SNDPMAYILTLLFTSLVVNEQSDIVTVIPMFISQLVLGGVLGFGIGYVSKILINKINL
                     DYEGLYVILVIAIMFFTFSATDFLHGNGFLAIYICGVYLGNQELLHKKKILQSFESFA
                     LLMQIILFLTLGLLVFPSQIVPVIGIGLIFSVFLMFIARPISVFISLLPFKIKNNSKL
                     FISWVGLRGAVPIVFATYPLIAGIQKADLIFDIVFFISLTSVIVQGSTVNIVAKWLRV
                     VMPANLKKRTLSDMERSDSVKSSLTKIVIPPGCSIIGKKIVDVQFPKTSIISYIIRDN
                     VYIIPNGSTILQENDQLYVLSENELALQEVYTRLEMYTN"
     gene            complement(1616..2887)
                     /locus_tag="P5243_01515"
     CDS             complement(1616..2887)
                     /locus_tag="P5243_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRS18153.1"
                     /translation="MKYIVFIYFFLYSYVSFGQKETALKHITALSSPQMYGRGYVHNG
                     VGVAADYIDSCFYSYKLQTCKTLEGYRQRVTFNVNMFPGIVTLCINDTIDLTPGLDFI
                     IASNSPAAKYKTDNIVKTTKLQIAQGKLSDVSLRNSILVLDERLCNVTNERIEEVTKG
                     FQALRYQKNEFKAVVEISNKKLTWDVSERVGTFPYFYVNSARVPTTISSIPYHVEQSY
                     IPKYTTYNICGFVPGTVYPDSLIVITAHYDHLGMMGSKTMFPGANDNASGVAMMLTLA
                     EYYAKHPPHKTIVFIALTGEEVGLQGSFACVQNPPFPLNKVNFLINIDLAGTGDEGIQ
                     IVNSTIYKQKAAIIDSLNALHTYMPAIKKRGEACNSDHCPFYMKGVPSFFIYTLGGIQ
                     AYHDIFDKPETLPLTRFNEYNALLKQFIENL"
     gene            complement(2896..4227)
                     /locus_tag="P5243_01520"
     CDS             complement(2896..4227)
                     /locus_tag="P5243_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRS18154.1"
                     /translation="MTQTKKKSGIIAKLLTFITNDIWSINIKKLSSGQSFLIRLVRVI
                     ILAVRGFNEDRCIEKASALTYYTLFSLVPIAAMAFGIAKGFGFDAYLEEYLTQKFHDK
                     QELLKQILEFSNSMLANARGGVIAGIGIVVLLWSVIKVLGNIESSFNEIWAVNKQRTF
                     VRKISDYLTIMLLAPIFIIISSSATSQISLYITSLQKTVPWVSEVYGLISIALNLLPY
                     SLIWLAFAFLYIVMPNTKVSFKSAFLGGVIAGIVFQVIQYFYIKFQVGVTSYNAIYGS
                     FAAIPLFLFWMQLSWIIVLMGAEIAYATQNVKNFEFEVETKQLSHSLKMKLSLWVMHR
                     IVLDFEQGLPPQTSEQLSNSLDIPHRITKMILNILLDAHLIHETKTGDEKSFGYSPAL
                     STDTLSVSYCIDSIHNAGINNIEIEHIEEFNTITKVFETWKVQNDMLLKRV"
     gene            complement(4231..>5172)
                     /locus_tag="P5243_01525"
     CDS             complement(4231..>5172)
                     /locus_tag="P5243_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRS18155.1"
                     /translation="SMNLLGGSMLLSGYYATPLKEKRQAKFTLDIKNFDVAQTFSTFN
                     TVQKLAPIAKYLNGSFSTKFEFSSLLQNDFMPDLKTLNGFGELSASAMGMKETSLQTF
                     AVNNLKQESLKNIIIKNVLLFFSIENGALKLKPFETSFNDIKTIIEGSTSIDQAIDYK
                     IAMQIPRNQLGGQANSVLNSLTSAAASKGVDAAVGDFVDLSILATGSVANPKFKPVLG
                     NMTSGVFNSIKDQAKLRLQQEQERLKAQAQQEAQAKLDAAKLQAQQEAERKKKELEAK
                     VQAEKQKQTEAAKQEVNKQTDALKKDAKKQLDGVLKR"
BASE COUNT         1789 a          918 c          734 g         1731 t
ORIGIN      
        1 aattattata attgtaccaa tacccactta tgggtattta cacgctaaga ataaaaatta
       61 attcgtatac atttcaaggc gcgtatatac ttcttgtaat gccaactcat tttccgataa
      121 aacatacagt tgatcatttt cttgtaaaat agtagaacca ttaggtataa tatatacgtt
      181 atctcgtatg atatatgaaa taattgaagt cttaggaaat tgaacatcta ctattttttt
      241 cccaattatt gaacaccccg gtggtattac aattttagta agcgatgatt ttacactatc
      301 tgaacgttcc atgtcagaca atgtacgttt ttttaggttt gccggcatta ctactcgcaa
      361 ccattttgcc acaatattta ccgtagaacc ttgcacaata accgatgtaa gtgaaataaa
      421 aaagactata tcaaaaatca aatctgcttt ttgaatacct gcaataagcg gatacgttgc
      481 aaaaactata ggaacagcac cacgtaaacc aacccatgat ataaataatt ttgaattatt
      541 tttaatttta aatggtagca aacttataaa aacactaatt ggccgcgcta taaacatgag
      601 aaatacagaa aatattaatc ctatgcctat taccggcact atttgacttg gaaatactaa
      661 taaaccaagt gttaaaaaca gtataatttg cataagcaaa gcaaaacttt caaacgattg
      721 tagtatcttc tttttgtgca ataattcttg attacctaaa tatactccgc atatatatat
      781 ggcaagaaaa ccgtttccat gtaaaaaatc ggttgccgaa aaagtaaaaa acataattgc
      841 tataaccaaa attacatata agccttcgta atcaagattg attttattaa ttagtatttt
      901 tgatacatag ccaatgccaa atcccaatac cccacctaat actaattgac ttataaacat
      961 ggggataact gtcactatgt cactttgttc gtttactact aacgatgtaa acaatagtgt
     1021 taaaatataa gccatagggt cgttactccc actctccaat tcaagagtag ggcgtatatt
     1081 attttttagc gctatattct ttgaacgtaa aattgaaaac acagcagcgg catcagtaga
     1141 tgaaactata gatccaagca gtaatgattc atatattgaa aaatctgtaa taaaccatac
     1201 aaaagttcca aatgtacctg ctgtaataag cacaccaagt gttgacaata atgtcccttg
     1261 ccccaataca ggacgtattg aagcataatc agtatccaat ccacccgaaa ataaaataaa
     1321 attcaatgct atgattccta taaattgagc aacatgtgga ttatcaaaat aaattccgcc
     1381 aataccttcg gaaccggcta gcataccaat tgaaataaac aatacaacaa ccggaatacc
     1441 gaatttgtat gatgttttac cagcaataag cgatataaat aataataatg aacctattag
     1501 taaaaaattt tgtgaggtaa tatccatttt tttgtgttta tctacaaata tacattttta
     1561 atcactaatt cataactata taaaatatct atagttataa atgaatagat taatcttaca
     1621 agttctcaat aaattgtttt aataaagcat tatattcatt aaaacgggta agcggcaaag
     1681 tttcgggctt atcaaatata tcatgatagg cttgaattcc accaagagta tatataaaaa
     1741 atgaaggtac tcctttcatg taaaacgggc aatgatcact attacatgct tcgcctcgtt
     1801 ttttaatagc aggcatatat gtatgtagag cgttcaatga atcaataatt gcagcttttt
     1861 gtttatatat tgtactgttt actatctgaa ttccctcatc gccggtacct gccaaatcta
     1921 tatttataag aaaatttact ttgtttaatg ggaacggcgg attttgcaca caagcaaatg
     1981 atccttgtaa tcccacctct tcacctgtaa gtgcaataaa gactatagtt ttgtgaggtg
     2041 gatgcttcgc ataatattct gccaaagtca acatcattgc aacgccactg gcattgtcgt
     2101 ttgctcccgg gaacatagtt ttacttccca tcattcctaa atgatcataa tgtgcagtaa
     2161 taacaatgag tgaatcggga taaacggttc ccggaacaaa accacatata ttatatgttg
     2221 tatattttgg aatatatgat tgctctacat gatatggaat tgatgaaata gtagttggca
     2281 cacgagccga attcacataa aaataaggaa acgtacctac acgttcagat acatcccacg
     2341 ttagtttttt attagaaatt tcgacaactg ccttaaattc atttttttga taacgtaacg
     2401 cctgaaatcc ttttgtaact tcttcaattc gttcatttgt aacattacac aatcgttcat
     2461 caagcaccag tatagaatta cgtaacgata catctgacaa tttaccttgc gcaatttgaa
     2521 gttttgttgt ttttactata ttgtcggttt tgtactttgc tgcaggagaa tttgaagcaa
     2581 ttataaaatc tagaccgggc gtcaaatcaa ttgtatcatt tatacataac gttacaatac
     2641 cgggaaacat attcacatta aatgttacac gttgcctata tccttctaag gttttacatg
     2701 tttgtaattt atacgaataa aaacatgaat caatataatc ggcagcaact cctactccat
     2761 tatgcacata tcctctacca tacatctgtg gcgatgataa tgcggtaata tgctttaatg
     2821 ctgtttcttt ttgaccaaaa ctaacataag aatacaagaa aaaataaata aaaactatgt
     2881 atttcataac gaacattaca ctcgtttcaa caacatatca ttctggactt tccacgtttc
     2941 aaagactttg gtaatagtat tgaattcctc aatatgttct atttctatat tgttgatacc
     3001 tgcattatga atagaatcaa tacaatatga gacagaaagt gtatcggtac ttaatgccgg
     3061 agaatatcca aatgattttt catcaccggt tttggtttca tgtatcaaat gcgcatcaag
     3121 taaaatattg agtatcattt tagtaatacg atgcggtata tcaagtgaat tacttaattg
     3181 ttcagacgtt tgtggcggta aaccttgttc aaaatctaaa acaattctat gcattaccca
     3241 caaagaaagt ttcattttaa gcgagtgact caattgttta gtttcaacct caaactcaaa
     3301 gtttttcaca ttttgagtag catacgcaat ttctgcaccc ataagaacta taatccatga
     3361 aagttgcatc caaaataaaa acaacggtat tgctgcaaaa ctaccataga tggcattata
     3421 tgaagttaca ccaacttgga attttatata aaaatactgt attacctgaa acacaatacc
     3481 ggcaattact ccacctaaaa aagctgattt aaaagatact tttgtatttg gcattacaat
     3541 atataaaaat gcaaatgcta accaaatcaa actatacggc aataaattta aagcaataga
     3601 aataagccca tatacttctg aaacccatgg tacagttttt tgtaatgaag ttatgtataa
     3661 agaaatttgt gaagtagcac tacttgaaat aataataaaa ataggtgcta ataacataat
     3721 tgtaagataa tcacttattt ttctaacaaa tgttcgttgt ttatttaccg cccatatttc
     3781 attaaatgaa ctttcaatat ttcctaagac tttaattact gaccataata acactacaat
     3841 acctatacct gcaatcactc caccacgagc attagctaac atagaattgc taaattcaag
     3901 tatttgctta agtagttctt gtttgtcatg aaatttttgc gtaagatact cttctaaata
     3961 tgcatcaaaa ccaaatcctt tggcaattcc aaaagccata gctgctatag gcacaagcga
     4021 aaataaggtg taataagtaa gagccgaagc cttttcaata catctgtctt cattgaaacc
     4081 acgaactgca agaataataa ctcgcactaa acgtataaga aatgattgtc cactcgaaag
     4141 ttttttgata ttgatactcc aaatatcatt agtgataaac gtaagtaatt ttgcaattat
     4201 tccacttttt ttcttagttt gagtcatatg ttatcttttt aatactccat ctaattgctt
     4261 tttagcatct tttttcagag catcggtttg tttatttact tcttgtttag ctgcttctgt
     4321 ttgcttttgc ttttcggctt gaacttttgc ttctaattcc tttttcttac gttctgcctc
     4381 ttgttgagct tgcaatttag cagcatctaa ttttgcctga gcttcttgtt gagcttgagc
     4441 tttaagtcgt tcttgttctt gttgtaaacg caattttgct tggtctttta tagaattaaa
     4501 cacacctgaa gtcatatttc caagaacagg tttaaattta ggattagcaa cagaccctgt
     4561 agcaagaata gaaagatcta caaaatcacc tacggcagca tctacccctt ttgatgcagc
     4621 tgctgatgtt aacgaattaa gcacagaatt ggcttgccca cctaattgat tccttggaat
     4681 ttgcatagca attttataat ctatagcctg atctattgaa gtagatccct caataatagt
     4741 tttaatatca ttgaaacttg tttcaaaagg ttttaacttc aaggcaccat tttctatact
     4801 aaaaaataga agtacatttt ttataataat attttttaaa ctctcttgtt tcaaattatt
     4861 aacagcaaag gtttgcaaag atgtttcttt cattcccata gccgatgcac taagttctcc
     4921 aaaaccattc aaggttttta aatcgggcat aaaatcgttt tgtaataatg acgaaaattc
     4981 aaattttgta gaaaatgatc cattcaaata tttcgcaatt ggagctaatt tttgaacagt
     5041 attaaatgta ctaaatgttt gtgctacatc aaaattttta atatcaagag taaatttagc
     5101 ctgacgtttt tcctttagtg gtgttgcata gtatccactt aacaacatac ttcctcccaa
     5161 caaattcatt ga
//
LOCUS       DAOTOW010000054         5163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13397_length_5163_cov_4.026038, whole genome shotgun sequence.
ACCESSION   DAOTOW010000054 DAOTOW010000000
VERSION     DAOTOW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13397_length_5163_cov_4.026038"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="P5243_01530"
     CDS             complement(<1..584)
                     /locus_tag="P5243_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18156.1"
                     /translation="MAQNSDSTQKQSLLDKTMPLFEWKIGSATISSFPSIGYDPSSGL
                     YGGILQVVAIPPKDSTKYSRSTSLVNHYTYSTNHWINLKSDLILYTRKGININTYAQF
                     LEAPDSYYGIGNDTLNTMPAQFNIQDFELRGDISKSIANTFFGGMVFDFSHTYTRAGD
                     TTALGLQIPAQKNKLLLGIGPHIAFDTRNNVNYPS"
     gene            complement(631..1821)
                     /locus_tag="P5243_01535"
     CDS             complement(631..1821)
                     /locus_tag="P5243_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRS18157.1"
                     /translation="MTSQKQLFLQLLGQTSPNPLLLEIERAQGIYMYTPDGKEIIDLI
                     SGVSVSNIGHCHTRVVEAVCNQAQSYMHLMVYGELIQTPQIQLAQLITSLLPSSLDSV
                     YFVNSGSEAVEGALKLAKRVTGRSKIISTHNAYHGSTHGAMSIMGNSNFTKSFEPLLP
                     NIDFIDFNCLENIEKIDTQTACVILEPIQSEAGIIEATQEFLTALRNKSNETGTLLIF
                     DEIQNGFGRTGTMFAFEHYGIIPDIICFAKGMGGGMPIGAFVSSKKNMDCFTCNPVLG
                     HITTFGGHPVSAAASYACIRELAENKEIIETIPQKSELFKTLLLKHPKVRNIRGKGLF
                     LAVELESSKQVHALISKGLEYGVLLDFFLFCDTHFRIAPPLIIDTNQIIESCNRIYRA
                     LDEI"
     gene            complement(1878..2579)
                     /locus_tag="P5243_01540"
     CDS             complement(1878..2579)
                     /locus_tag="P5243_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRS18158.1"
                     /translation="MILRSYPDIGDVYYTIQSKNKRISLRITKKNTVEVCVPHVNLLQ
                     RAEEFVLEKKTWILKKLSLPKPQPIIFSKNTKEFTPQHSLEIIPSHTSKTSESIENNK
                     VIISLSHIHDIESEGVQKFIAKIREQVLRDEAYTYIPNRVKELSKKHGFTYNSIRILH
                     AKTRWGTCDARNNLIFTCYIMLLPHYLIDFIILHELCHTIHKNHGQQFHTLLANITEQ
                     KKQTYERELKQYRIL"
     gene            complement(2576..3514)
                     /locus_tag="P5243_01545"
     CDS             complement(2576..3514)
                     /locus_tag="P5243_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS18159.1"
                     /translation="MEAIIDPIERSILEAELSNDKFLRKTSFGSNLLYTINYHNAPHV
                     MLEIGRLREIAFREAGGGTGKSADIDSYDTDPQFPYQQLIVWDPAHKQILGGYRYILC
                     KNARDTDGVYHLATKGLFNFSETFQTDYLPYVIELGRSFVHHEYQSGVRGRKGIFVLD
                     NLWEGLGAIMALNPQIKYLFGKVTMYLHYDKLGRDYILYFMKKNFGDKQGLLTPKSPL
                     AFHNPEEELAKVFTGNTPEEDYKILFHKVRERKHNIPPLISSYVNLSNTMKSFGTALN
                     TDFGDVEETGILVTRDEIVPDKLERYVYSYKKESDI"
     gene            complement(3516..4337)
                     /locus_tag="P5243_01550"
     CDS             complement(3516..4337)
                     /locus_tag="P5243_01550"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRS18160.1"
                     /translation="MNNTNIHIDIDKVIQQKNPRLYSLLPGFVLSYIRKIIHQNDLNR
                     DLQKLAPYKGAEKMRKFLEFQNITTKLQNIEHIPQSGRFIFVSNHPFGGPDGIILIAT
                     ISRFFPKIHFLVNDILMHLPDMEEIFLPINKHGRNSKEYYEIIDNAYSSDAQIIVFPA
                     GIVSRKINGTITDLEWQKSCVSYAKKYKRDIIPLYIHGKNSNFFYNLASLRKRLRIRA
                     NIEMFFLADELYKHNNEEITITFGKPISYTHFTDSKKDIEWAQELKQITYALANK"
     gene            4476..>5163
                     /locus_tag="P5243_01555"
     CDS             4476..>5163
                     /locus_tag="P5243_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS18161.1"
                     /translation="MKIIRFAVVGLGHIGKRHAQMIKQDPDAQLVAYCDIKPREELNL
                     QETDVPFFSSIEEMLNSGIEIDVVNICTPNGYHAQQSILALNKKCHVVCEKPMALTKA
                     DAESMIYKSLEVSRNIFCVMQNRYSPPSVWLKEIISNNILGNPYMVQLNCYWNRDERY
                     YKKGGWHGNAQLDGGTLFTQFSHFIDIMYWLFGDITNIRANFKDFNHQHLTDFEDSGF
                     VSFDFVNGGMG"
BASE COUNT         1623 a          775 c          901 g         1864 t
ORIGIN      
        1 gaagggtaat tcacattgtt tcttgtatca aaagcaatat gaggcccaat acctaaaagc
       61 agcttatttt tttgtgcggg aatttgtaat cctaatgctg tagtgtcgcc ggcacgggta
      121 tatgtatgtg aaaaatcaaa caccattcct ccaaaaaagg tgttggcaat agattttgaa
      181 atatcgcccc gtaactcaaa atcttgtatg ttaaattgag caggcatggt atttaaagtg
      241 tcattcccta taccataata tgaatcggga gcctctaaaa attgagcata tgtgtttata
      301 tttattcctt tgcgtgtata gagaattaaa tctgatttga gattaatcca atgattagtt
      361 gaatatgtgt aatggttcac taatgatgtg gaacgactgt attttgtaga atctttcggg
      421 ggaatagcta caacttgtag tatgccacca tagagtccac ttgaggggtc atatccaata
      481 gaaggaaaag agctgatggt tgcagaacct attttccatt caaatagcgg catggttttg
      541 tcaagcaatg attgtttttg agttgaatca gaattttgtg ccaaaatagt attgcacaaa
      601 caagacataa atacaagtag taaatacaat ttatatttca tcgagagcac ggtaaatacg
      661 attacaagat tcaattattt gattagtatc tattataagt ggcggagcta tacgaaagtg
      721 tgtgtcgcaa aataagaaaa agtcaagcaa tacaccatat tctagtcctt ttgatataag
      781 agcatgaact tgctttgatg attctaattc tacggcaagg aataatccct taccacgaat
      841 atttcgaact ttggggtgtt tgagtagaag tgttttaaat aattcagact tttgtggtat
      901 agtttcaata atttctttat tttctgcgag ttcacgtata caagcataag aagctgctgc
      961 agaaacagga tgtccgccaa atgtggtaat gtgtcctaaa acaggattac aggtaaagca
     1021 atccatattt tttttgctcg aaacaaaagc acctattggc ataccgccac ccataccttt
     1081 tgcaaaacaa attatatcgg gaattattcc gtaatgttca aaagcaaaca tggttccggt
     1141 tcgtccaaat ccattttgaa tctcgtcaaa tatcagcagt gttcctgttt cattactttt
     1201 gtttcgcaat gcggttaaaa attcttgtgt tgcttcaatt attcctgctt cactttgaat
     1261 tggttcaaga attacacacg cagtttgagt atcaattttt tcaatatttt ctaaacaatt
     1321 aaaatctata aaatcaatat tgggtaataa tggttcaaac gattttgtaa aattgctgtt
     1381 acccattata ctcattgctc catgagtact gccatggtat gcattgtgtg ttgaaattat
     1441 tttggaacga ccggtaacgc gttttgccaa ttttaaagca ccttctacag cttcgctccc
     1501 cgaatttaca aaatatactg aatcaagaga ggaggggagc agtgaggtaa ttaattgtgc
     1561 tagttgtatt tggggagttt gtattaattc accatatacc ataagatgca tatatgattg
     1621 tgcttggttg catacagctt ctacaactcg tgtatggcaa tgtcctatat tactaacaga
     1681 tactcctgaa ataaggtcaa ttatttcttt accatcggga gtgtacatgt aaataccttg
     1741 tgctctttct atttctaaaa gcaaaggatt tggcgaagtt tgtcctaaca attgtaaaaa
     1801 aagctgtttt tgtgaagtca tatatatctt ttttgcaaag atatttaaat aatatcagtt
     1861 acaaatagta ttttgtgtta tagtattcta tactgtttta gttctcgttc gtaagtttgt
     1921 tttttctgtt ctgtaatgtt tgcaagtaat gtgtgaaatt gttgaccatg atttttgtgg
     1981 atagtatgac ataattcgtg gagaattata aaatcaataa ggtaatgagg taataacatt
     2041 atataacaag taaaaataag attattacga gcatcgcatg ttccccaacg agtttttgca
     2101 tgtaatattc gaatggaatt atatgtaaat ccatgctttt tacttagttc ttttactcga
     2161 ttgggaatat atgtatatgc ttcgtcacgt aatacttgtt ctctaatttt tgcgataaat
     2221 ttttgaactc cctctgattc aatatcgtga atatgcgaaa gagatatgat tactttatta
     2281 ttttcaatac tttccgaggt tttagatgta tgtgatggta taatttctaa agaatgttga
     2341 ggtgtaaatt ctttggtgtt tttgctaaaa ataataggtt gtggtttggg taatgataat
     2401 tttttaagta tccatgtttt tttttctaat acaaattctt cggctctttg taataaattc
     2461 acatgcggca cacaaacttc tactgtgttt ttttttgtta ttcgtaaact tatccgctta
     2521 ttttttgatt gtatggtgta atatacatct cctatgtcag gatatgaacg aagtatcata
     2581 tatcggattc ttttttataa gaatatacgt atcgttctag tttatctggt actatttcat
     2641 cgcgtgttac taatattccg gtttcttcta catcgccaaa gtcagtattg agagcagtcc
     2701 caaaactttt cattgtattg cttaagttta cgtatgagct tataagtggt ggaatattgt
     2761 gttttcgttc acgtactttg tgaaataaaa ttttgtaatc ttcttcgggc gtattgcctg
     2821 taaaaacttt tgctaattct tcttccggat tatgaaatgc taatggtgat ttaggagtca
     2881 aaagaccttg tttgtcacca aaatttttct tcataaaata taaaatataa tctctgccaa
     2941 gcttatcata gtgtaaatac atggtaactt taccaaataa atattttatt tgaggattta
     3001 aagccattat agcacctaac ccttcccata aattatcaag tacaaatata cctttgcgac
     3061 cacgaactcc cgattgatat tcgtgatgta caaacgaacg ccctaattca attacatagg
     3121 gtaaatagtc tgtttgaaat gtttcggaaa aattaaaaag tccctttgtt gctaaatgat
     3181 aaaccccatc agtatcacga gcatttttgc atagtatata tcgatatcct cccaaaattt
     3241 gtttgtgagc aggatcccaa actattaatt gttgatatgg aaattgtgga tcagtatcat
     3301 acgaatctat atcagcagat ttgccggtac ctccacctgc ttcgcgaaaa gctatttcgc
     3361 gtaagcgacc aatttccaac attacatgtg gtgcgttatg atagtttatg gtatagagta
     3421 aatttgaacc aaagctagtt ttacgtaaaa atttgtcatt acttaattct gcttctaaaa
     3481 tgcttcgttc tatggggtca attattgctt ccatattatt tgttcgctaa tgcgtatgtt
     3541 atttgtttta attcttgtgc ccactctata tcttttttag aatcagtaaa gtgagtataa
     3601 gaaataggtt taccaaaagt tatggtaatt tcttcattat tatgtttata taattcatca
     3661 gctaaaaaga acatttcaat atttgctcgt atcctcaatc gttttcttag cgacgctaaa
     3721 ttataaaaga aatttgaatt tttaccatga atatacagtg gaattatatc acgtttatat
     3781 ttttttgcgt aactcacaca tgatttttgc cattctaaat cggttatagt accattaatt
     3841 ttacgcgaaa ctattcctgc cggaaataca attatttgag catctgatga ataagcgtta
     3901 tctataattt catagtattc ttttgaattt ctgccgtgtt tattgatagg taagaaaatt
     3961 tcctccatat caggtaaatg cattaaaata tcatttacta aaaaatgaat ttttggaaaa
     4021 aatcgagaaa tagttgctat aagtataatt ccatcgggtc ctccaaacgg gtggtttgat
     4081 acaaatataa aacgaccgct ttggggaata tgttctatat tttgtaattt agtagtaatg
     4141 ttttgaaatt caaggaattt tctcattttt tcggctcctt tgtagggagc aagtttttgt
     4201 aaatctctat ttaaatcatt ttgatgaatg atttttctga tatatgataa aacaaaaccg
     4261 ggtaaaagtg aatatagacg aggatttttt tgttgtataa ctttgtctat atcaatatgt
     4321 atattagtat tattcattaa atccttgcaa taggaatgca aatgtacgcg taaaaataat
     4381 ataattgaag ttttttttta tttttgtata ttatagagtc actacaaata ctactgttga
     4441 aagtattatt tgttataaac aaacatacaa tatatatgaa gataattcga tttgctgtag
     4501 ttgggctggg acacataggt aaacgccatg ctcaaatgat aaaacaagac cccgatgccc
     4561 aattggtggc atattgcgat ataaaacctc gtgaagaact taatttgcaa gaaaccgatg
     4621 tgcctttttt tagttcaata gaagaaatgc ttaattcggg tattgaaata gatgttgtaa
     4681 atatttgtac tcctaacggg tatcatgcac agcaatcaat tttggctttg aataaaaaat
     4741 gccatgtagt atgtgaaaaa ccaatggctc ttactaaagc tgatgcagaa tcaatgattt
     4801 ataaatctct tgaggtttca agaaatatat tttgtgtaat gcaaaacaga tattctccgc
     4861 catcggtgtg gctcaaagaa ataatatcga acaatatttt aggtaatcct tatatggtgc
     4921 aacttaactg ttattggaat cgtgatgaac gatattataa aaaaggaggg tggcatggta
     4981 atgcccaatt agatggagga acattgttta cacagttttc acattttata gatataatgt
     5041 attggttgtt tggcgatatt actaatatac gtgccaactt caaggatttt aatcatcagc
     5101 accttaccga ttttgaagat agtggttttg tgtcgtttga ttttgtaaac ggtggtatgg
     5161 gaa
//
LOCUS       DAOTOW010000055         5148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13438_length_5148_cov_2.549971, whole genome shotgun sequence.
ACCESSION   DAOTOW010000055 DAOTOW010000000
VERSION     DAOTOW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13438_length_5148_cov_2.549971"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="P5243_01560"
     CDS             <1..619
                     /locus_tag="P5243_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS18162.1"
                     /translation="VEGKIVDETQKPVQANVEFNDNETNKVVVKTESDKTTGEYKAQL
                     TEGKNYGMYVSREGYIFHSENINLSDENAIKSTQSNIKKNIEMKPAVPGNSIVLNNIF
                     FDYGKSTIRESSIPELDRVVAFLQENPRLRVEISGHTCNTSSWGFNKKLSEARALSVT
                     NYLVQKGIARNRIVAVGYSYDKPIASNATEEGKQKNRRTEFKIIQ"
     gene            complement(609..1058)
                     /locus_tag="P5243_01565"
     CDS             complement(609..1058)
                     /locus_tag="P5243_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18163.1"
                     /translation="MNERLLISIPFSNKRLYISSLLLLLATIPFIVYSNYSTKVGFLY
                     VILGFYVCLGVYLLFVFARTKTAAIRIYSSKIIDNHRITNFSRVIQKHDIQSIELRET
                     LLRSGPFSFIVIKCLSNSKEIHIHTDSIEIQLHELYKMLQDWFTTIE"
     gene            complement(1062..1940)
                     /locus_tag="P5243_01570"
     CDS             complement(1062..1940)
                     /locus_tag="P5243_01570"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRS18164.1"
                     /translation="MNKVELVYDAQAGVGEGALWDYENNRLLWIDITGKALFIYNPQL
                     KSMNSYAMPKEIGTVVPIDSDNVLLALADGMYEFHISTKKLVQKSRPDIDTSLVRFND
                     GKCSPDGKLWVGTMDHGVSKPIAALYRVSGDYSIRQMVDGVTVSNGITWSPDGKTMYY
                     IDSPTYTVVAYDYDINRSEISNKRIIIHTPKEWGTPDGNTIDSEGMLWVAHWGGGLVS
                     RWNTTTGELLDTIRVPSPNVTSVALGGSNMKTLFITTARNWMKPGDEQKYPHAGGLFA
                     VEVTVPGIKSHYFKIK"
     gene            complement(1999..3936)
                     /gene="dnaG"
                     /locus_tag="P5243_01575"
     CDS             complement(1999..3936)
                     /gene="dnaG"
                     /locus_tag="P5243_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRS18165.1"
                     /translation="MIHPDTVEQIIDRARIEEVISDFISLKKSGANYKGCCPFHNEKT
                     PSFMVSPAKGIFKCFGCAKGGNAVSFVMEHERLSYVDALKYLAKKYNIEVIETQVSDE
                     DKQKKDVRESLQIVNNFANTYFKQKLHESNEGRAIGLSYFKERGFTDETIAKFELGYS
                     HDGKDIFTQEALKQGYKQEFLERTGLTVSGETNSGQTYFVDRFRGRVMFPIHNLSGNV
                     IGFGGRIVGSDPEKKLAKYLNSPESEIYNKSKTLYGIYFARTEITKKDECILVEGYTD
                     VMSMHQSGITNVVASSGTSLTLEQIQLIKRFTNNILVIYDGDSAGIKASFRGINMILE
                     EGLNVKVLLLPDGDDPDSFAKKHTAQELQTYIVENKTDFVTFKTRLFAEESKNDPIER
                     AKLINDIVHSIAVIPDSITQSVYVSECSKTLQISESILFEQIQKIKNAKGGKDSALYI
                     KQSAPVPKPVIQKSQVHATMSNELYYNEFDIIRLLLEYGEYDVFIHSVETKVKYYMYQ
                     EIVEGEMLLQNPVFSKIFDTYYTYMQQGSTDLYKHLLYHPDEEITKIVVEINSKDKKL
                     SPLWKRKESMVETEDKKLSELLIDTIGAYKKRRLDEEAKHLNELLKEEKDITRIIEIS
                     GRLTIVKKLITSISKELHHVS"
     gene            4249..>5148
                     /locus_tag="P5243_01580"
     CDS             4249..>5148
                     /locus_tag="P5243_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRS18166.1"
                     /translation="MSQIETKSLYDPSYEHDACGIGFIANLKGVKSHKTIDDALKMLE
                     NMEHRGAVGFEENTGDGAGVLIQMPHNFFKITCKEIGIDLPDYGKYGVAMIYFPKNVV
                     MREACRKIFNDSIEHLGLELLGYRNVPVSNKTIGKTAISTEPKIEQAFIVAKDSYLPS
                     DALERKLFVLRNWSYHQFCQQFPENKDDFYIVSSSYKNITYKGQLTTYQVREYFLDLS
                     DERLDSAFAMIHSRFSTNTFPKWKLAQPFRYIAHNGEINTIRGNVNWAHCNQTLFESS
                     LFTKDEIEKLLPICDHENSDSANL"
BASE COUNT         1795 a          888 c          725 g         1740 t
ORIGIN      
        1 tgtagaagga aaaatagttg acgaaactca aaaacctgtt caagcaaatg tagaatttaa
       61 tgataatgaa actaataaag ttgttgtaaa aacagaatcg gataaaacta ccggagaata
      121 caaagcccaa cttaccgaag gtaaaaatta cggaatgtat gtaagccgtg aaggttatat
      181 atttcactca gaaaacataa atttgagtga tgaaaatgca attaaaagca cacaaagcaa
      241 tataaagaaa aatattgaaa tgaaacctgc cgttccgggt aatagtatag tattaaacaa
      301 catatttttt gattatggta aatcaactat acgagaatca tcaattcctg aattagacag
      361 ggttgttgca tttttacaag aaaatccgcg cttacgtgtt gaaatttctg gacatacttg
      421 taatacaagt tcatggggat tcaacaaaaa attatctgaa gcacgagctt tatctgttac
      481 taattattta gtacaaaaag gaatagctcg caatagaatt gtagctgtag ggtattcata
      541 cgacaaaccc attgccagca atgcaaccga agaaggtaaa caaaaaaatc gacgtaccga
      601 attcaaaatt attcaatagt agtaaaccaa tcctgtaaca ttttatataa ttcatgcaat
      661 tgtatttcaa tagaatcggt atgaatatga atttctttag aatttgacaa gcatttaata
      721 acaataaaag aaaaaggacc tgaacgaagt aacgtttctc gtaattctat agattgaata
      781 tcgtgttttt gaattactct tgaaaaattt gtaattctat gattgtcaat aatttttgac
      841 gaataaattc taattgcagc tgtttttgtt cgagcaaata caaataacaa ataaacgcct
      901 aaacacacat aaaaacccaa aattacatac aaaaaaccaa cttttgtaga ataattggaa
      961 tatactataa aaggaatagt tgccaacaac aacaacaaac tacttatata taagcgttta
     1021 ttactaaaag gaatagaaat aagaagacgt tcgttcatac attacttgat tttaaaataa
     1081 tgagatttaa taccgggaac ggttacttct acagcaaata aacctccggc atgaggatat
     1141 ttttgttcat cacccggttt catccaattg cgagcagtgg taataaataa agttttcata
     1201 ttactaccac ctaaagctac tgaagtaaca ttgggcgaag gaactctaat agtatcaagt
     1261 aattcaccgg ttgtagtatt ccaacgacta accaatcctc cgccccagtg agcaacccac
     1321 aacattcctt ctgaatctat ggtatttccg tcgggtgttc cccactcttt gggtgtatgt
     1381 atgataattc gtttatttga aatttctgaa cgatttatat cataatcata cgcaaccaca
     1441 gtatacgtag gagaatcaat ataatacata gtttttccat cgggcgacca cgtaatacca
     1501 tttgaaacag ttactccatc taccatttgt ctgatactat aatcaccaga aactctatac
     1561 aaagcagcta ttggcttgct tacaccatga tccatagtac caacccacaa tttaccatcc
     1621 ggagaacact ttccatcatt aaatcgcacc aaacttgtat caatatcggg tcgtgatttt
     1681 tgcactaatt tttttgttga aatatgaaac tcatacattc catctgctaa tgccaataat
     1741 acattatctg aatcaatagg aactacagtt ccaatctctt ttggcattgc atatgaattc
     1801 atacttttta attgtggatt atatataaaa agcgcttttc cggtaatatc tatccataac
     1861 aatctattat tttcataatc ccataaagct ccctcaccta cacctgcctg tgcatcataa
     1921 acaagttcaa ctttattcat ttttgaacac tgtgttaata gtgttaatac tggtataata
     1981 gccataaata catataattt acgatacatg atgtaattct tttgatattg atgtaattaa
     2041 ttttttaact attgttaatc gtccgcttat ttctattatt cgtgttatat ctttttcttc
     2101 ttttaataat tcattcaaat gctttgcctc ttcatcaagt cttcgttttt tataggctcc
     2161 aattgtatca ataagcaatt cgcttaattt tttatcttcg gtttctacca tactttcttt
     2221 acgtttccat aaagggctca attttttatc tttgctgttt atttcaacta caattttagt
     2281 aatttcttca tcaggatgat acaacagatg cttatacaaa tcggtacttc cttgttgcat
     2341 atacgtataa taagtatcaa atatttttga aaacaccgga ttttgcagta acatctcacc
     2401 ttctacaatt tcttgataca tataatattt aaccttagtt tcaacagaat gaatgaacac
     2461 atcatattct ccatattcca atagtaaccg aataatatca aattcattgt aatataattc
     2521 atttgacatt gttgcatgca cctgcgattt ttgaattaca ggtttaggca caggtgcact
     2581 ttgctttata tataaggcag aatctttgcc tccttttgca ttctttatct tttgaatttg
     2641 ttcaaatagt atactttcag aaatttgaag agttttacta cattccgaca catatacact
     2701 ttgtgtaatt gaatctggaa ttacagctat tgaatgtaca atatcattaa ttaatttagc
     2761 tcgttcaata ggatcatttt ttgattcttc ggcaaacaaa cgcgttttaa atgttacaaa
     2821 atcagtttta ttttcaacaa tatatgtttg taattcttgc gctgtatgtt ttttagcaaa
     2881 tgaatcaggg tcatcaccat caggtaatag aagtactttt acattgagtc cttcttccaa
     2941 aatcatattt atacctctaa atgaagcctt aatgccggca gaatcgccat cataaattac
     3001 taaaatatta ttggtaaatc gttttataag ttgaatttgt tctaaggtaa gcgaagttcc
     3061 tgatgatgca actacattgg taataccact ttgatgcata ctcataacat cggtatatcc
     3121 ctctaccaat atacattcat ctttttttgt aatttctgtt cgggcaaaat aaattccgta
     3181 taaggtttta ctcttattat atatttctga ttccggagaa tttaaatatt ttgctaattt
     3241 tttttcagga tcacttccaa ctattcttcc gccaaatcca ataacatttc ccgagagatt
     3301 atgtattgga aacataactc ttccacgaaa tctatcaaca aagtatgttt gtccagaatt
     3361 agtttcacca ctcacagtaa gtccggtacg ttccaaaaat tcttgcttat agccttgttt
     3421 tagagcttct tgcgtaaata tatctttacc gtcgtgacta tacccaagtt caaattttgc
     3481 aattgtttca tctgtaaatc cacgttcttt aaaatatgat aacccaatag ctcgaccttc
     3541 attactttca tgaagttttt gtttaaaata ggtatttgcg aaattattta caatttgcaa
     3601 actttcacga acatctttct tttgtttatc ttcatcactt acttgtgttt ctataacttc
     3661 tatattatat tttttagcaa gatattttaa cgcatctaca taactcaatc gttcatgctc
     3721 cataacaaaa cttacagcat ttccgccttt ggcacagcca aaacatttaa aaataccttt
     3781 agccggagaa accataaaag aaggtgtttt ttcattatga aacggacaac accctttata
     3841 attagctccc gatttcttta aagaaataaa atcactaata acctcttcaa tgcgagctct
     3901 gtcaattatt tgttcaaccg tatcgggatg tatcatgaat taaatctctc tttgtaatac
     3961 acttaaaaat atatggtaaa aataaacact atacacatgt aaaaaaaatt ttttgtttgt
     4021 gtgttatcca caattacaca tattttatat ataccactaa aaatcagagc tatacaaaat
     4081 aacatgatga attgcagtat aaaaatatgt taaaaattat aataaaaaac ttaaaaaatc
     4141 ttttgaatcg ttatatgaag tattattttt gctcaaaatt cgtaatcgaa aaatatgatt
     4201 tttcggtaaa aaaaatagga tagagtatac aatttatata taaaacagat gagtcagatt
     4261 gaaacaaaat ctttgtatga tccaagttat gagcacgatg cgtgtgggat tgggtttata
     4321 gcaaacttaa aaggtgtaaa atcgcataaa acaattgacg atgccttaaa gatgctagaa
     4381 aatatggagc atagaggtgc tgttgggttc gaagaaaaca ccggagacgg tgccggagta
     4441 ttaattcaaa tgccgcataa tttctttaaa attacgtgta aagaaattgg tatagattta
     4501 cctgattatg gtaaatacgg cgtagcaatg atatacttcc ccaaaaacgt tgtaatgcgt
     4561 gaagcgtgtc gtaaaatatt taatgattca atagaacatt taggcttaga attattagga
     4621 tatcgtaatg taccggtttc aaataaaacg attggaaaaa ctgcaattag taccgaacca
     4681 aaaattgaac aagcatttat agttgctaaa gacagttact taccaagcga tgcattggaa
     4741 cgcaaattat ttgttttacg aaattggtca taccaccaat tctgtcaaca atttccggaa
     4801 aataaagatg atttttatat cgtttccagt tcatataaaa acataacata caaaggacaa
     4861 ttaacaacct atcaggttcg tgaatatttt cttgacttaa gtgatgaacg attggattct
     4921 gcttttgcaa tgattcactc tcgtttttca acaaacacat ttcctaaatg gaaattggct
     4981 caaccattcc gttatattgc tcacaatggt gaaataaata caatacgcgg taatgtaaat
     5041 tgggcacact gtaaccaaac attatttgaa tcatcattat ttactaaaga tgaaatagaa
     5101 aaactattac ctatttgcga ccatgaaaat tcagattctg ccaacctt
//
LOCUS       DAOTOW010000056         5135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13478_length_5135_cov_3.392913, whole genome shotgun sequence.
ACCESSION   DAOTOW010000056 DAOTOW010000000
VERSION     DAOTOW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13478_length_5135_cov_3.392913"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="P5243_01585"
     CDS             <1..1684
                     /locus_tag="P5243_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HRS18167.1"
                     /translation="YADLGFKTVSTNPCGEIPLCPYDSCRLLAINLYGYVENPFTPQA
                     SFNFPLLKKHVAYAQRMMDNIIDMELEKIDAIIEKIDSDPENFEIKRVEKNLWEKIKE
                     KAIEGRRTGIGITAEGDMLAALGLRYGSDDAIDFATEVHKTLAIEAYRASMEMAKLRG
                     PFKIFNPDRERNNPFLLRIKAADEQLYNDIMNYGRRNISMLTIAPTGTTSLMTQTTSG
                     IEPVFMVSYKRRKKVNPNDKNVKIAYVDEVGDSWEEYRVFHHRFITWLVANGYDANYA
                     QTCSDQELQALVEKSPYYKATANDVDWIKKVEMQGKIQKWVDHSISVTINLPETAKEE
                     LVSQLYIKAWEVGCKGVTVYRDGSRSGVLVSDKKSDKKSQDAGMPYIPPQRPTELEAD
                     VVRFYNNKELWVAFVGMLDGHPYEIFTGKSDEDVLPIPKSVTKGKIVKVKVDGVSRYD
                     FQYIDKHGYSNTFQGLSRMFDKEFWNYARLISGILRHRMPIESVVNLISSLSVDSESI
                     NTWKNGVERALKKYIPDGTKVSSGKTCECGSTNLIYQEGCLICQDCGASKCG"
     gene            1810..2520
                     /gene="pssA"
                     /locus_tag="P5243_01590"
     CDS             1810..2520
                     /gene="pssA"
                     /locus_tag="P5243_01590"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRS18168.1"
                     /translation="MIRNNIPNLITLCNLMCGVSAVIVAFSGIIPFEVASILVFAGAV
                     FDFFDGFAARVLNAKSAIGKELDSLADLITFGFAPAAILSAVIQHLLFGTVFPDLDTI
                     VWQEYILILTPFIIVPFSALRLAKFNVDERQSHTFIGLPTPANALFFAAIIFYSDLSP
                     VLLVFLSIAFSLLLVSEIPMFSLKFATYSFSENSFRYIFIALSIVAFIVFNLLSLPFI
                     ILAYILSSIVLYIISKRK"
     gene            2524..>5135
                     /locus_tag="P5243_01595"
     CDS             2524..>5135
                     /locus_tag="P5243_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18169.1"
                     /translation="MDYKHILEHDDWQRIFDALPFPVYILHKDYTLYFANKATLKLDE
                     YVNKHIFSLPCYKASQLHKNEINTCLYRNKEHITQSISHTIHVPEIDTYFHITCSPLF
                     DKTGKLDKIIHVATDISEVYEIKNQAVEHELQYKKLFENHISGFGLHKILCDDSGKPI
                     DYMFLEVNTAFERMTGLKASEIIGKRVKEIFPQIEDYWIENYGRVALNGEPFSFQNYS
                     QALDKYFEVVAYSPKHMHFAVVFNDITENHKNLQTIKEQEERLHAIINQAPDAVYLSD
                     MQGRFIVVNQTACEENGYTIDEMLNMSVFDIDINIISKNEIMSIWETMPLHTSQTLIT
                     KHKRKNSTTYPVEIRIAKILLDNKPHIIGFAQNVTQRVTQERELQLHNARLESLYAIS
                     QHQANEKAVFLDFVLDEVIKLTESSIGYIYYYNEDTELFTLNSWSKDVLDACTIVEKQ
                     ITYELEKTGIWGEVVRQRKPIIINDFAAQNLLKKGYPEGHVHLSSFASAPIIVDNKIV
                     AVVGVANKTTDYTQIDIMHMQIVMDSAWKIVERHTLFEELVKAKEIAEENNALKTAFL
                     ANISHEIRTPMNAILGFSELLSADNQSIEEREYFISIIKQSGKQLLRVINDIIDIAKI
                     SANAIKPDMRICDVLHIINNSIEIVKQQQYMKLQNKIQIHIQEHFPQYIIQSDPVRLQ
                     QIFTNLISNAVKYTPQGTVEIGSIPYTSDYICLYVKDTGIGIPKEFHEKIFELFRQVH
                     ANQTIEGTGIGLNITQGLIHLLGGSIWLESVEGKGTTFFITIPNTQIENVIDTTSKVS
                     LYSIPDWSTKTICIAEDNIHSYKLLEIILAPTQISILYAKNGAELLEIYAAKSHNIDL
                     ILLDIHMPVMNGVET"
BASE COUNT         1865 a          715 c          874 g         1681 t
ORIGIN      
        1 ttatgctgat ttaggattta aaacggtttc tacaaatcca tgtggcgaaa taccattatg
       61 cccctatgat agttgtcgtt tacttgctat aaatttatat ggatatgttg aaaatccatt
      121 tactcctcaa gcatcgttta attttccatt attaaaaaaa catgttgcat atgcacaaag
      181 aatgatggat aacataatag atatggagtt ggagaaaatt gatgcaatta tagaaaaaat
      241 agatagtgac cccgaaaatt ttgaaattaa acgtgtagaa aagaatttat gggaaaaaat
      301 taaggaaaaa gctatagaag gtcgcagaac tggtataggt attactgcag aaggtgatat
      361 gcttgctgct ttaggattac gctatggttc cgatgatgct atagattttg caacagaagt
      421 gcataaaaca cttgctatag aagcgtatag agcaagtatg gaaatggcaa aattacgtgg
      481 accgtttaaa atatttaatc ctgacagaga acgaaataat ccatttttac taagaataaa
      541 agctgccgac gaacaattat acaatgatat aatgaattac ggtcgtagaa atatttctat
      601 gcttacaatt gctcctaccg gcactacaag tcttatgact caaactactt cgggtataga
      661 accggtattt atggtttcat ataaacgaag aaaaaaagta aatccaaatg ataaaaatgt
      721 aaaaatagca tatgttgacg aagtaggaga ttcttgggaa gaatatcgtg tatttcatca
      781 tcgttttatt acatggttag tagcaaacgg atacgatgca aattatgcac aaacatgttc
      841 tgaccaagaa ttacaagctt tggttgaaaa atcgccatac tataaagcta cggctaacga
      901 tgtagattgg attaaaaaag ttgaaatgca aggtaaaata caaaaatggg ttgaccactc
      961 aattagtgtt accataaatt tacctgaaac agcaaaagaa gaacttgtta gtcaattata
     1021 cataaaagca tgggaagttg gttgtaaagg agtaactgta tatcgtgatg gttctcgttc
     1081 gggagtattg gtttctgata aaaaatctga taaaaaatca caagatgcag gtatgccgta
     1141 tattccgcct caacgtccta ccgaattaga agctgatgtg gtacgttttt ataacaataa
     1201 agaattatgg gtagcctttg ttggtatgct cgatggtcac ccatacgaaa tatttacagg
     1261 aaaaagtgac gaagatgtat taccaattcc aaaatcggta actaaaggaa aaattgtaaa
     1321 agtaaaagtt gatggagtgt ctcggtatga ttttcaatat attgataaac atggatatag
     1381 taatacattt caaggtttgt ctcgaatgtt tgacaaagaa ttttggaatt atgcacggtt
     1441 aatttctggt attttacgac atagaatgcc aatagaaagc gttgtgaatc ttatatcatc
     1501 attaagtgtt gatagtgaat caattaatac gtggaaaaat ggtgtagaac gtgctctcaa
     1561 aaagtatata cctgatggca ctaaagtaag ttcaggtaaa acatgtgaat gcggaagtac
     1621 aaacttaatt tatcaagagg gttgtcttat ttgtcaagat tgcggtgcaa gtaaatgcgg
     1681 atgatacata aaaaaagtgt atgtaaaaat tgggataaag atttggtttc tttatccctt
     1741 ttttttatat atttacacac atgtaagaaa gaattctaca gataaatatt ttacttaagt
     1801 ttctttagta tgattcgtaa caatataccc aatcttatta ctttgtgcaa cttaatgtgt
     1861 ggagtaagtg ccgtaatagt agcattttcg ggaattattc cttttgaagt tgctagtatt
     1921 ttggtttttg caggtgcagt attcgatttt tttgatggat ttgcagctcg tgtgctcaat
     1981 gcaaaatcag ctataggtaa agaacttgat tcgttggcag atcttattac ttttggtttt
     2041 gctcctgctg ctattctatc agctgttatt caacatttat tatttggaac ggtttttcct
     2101 gatttagata ctattgtatg gcaagaatat atactcattc tcacaccttt tattatagtg
     2161 ccattttctg ctttacgtct tgcaaaattt aatgtagatg aacgtcagtc gcatacattt
     2221 atagggttac ctactcccgc aaatgctcta ttttttgccg caattatttt ttattccgat
     2281 ttatcaccgg ttttattagt gtttttaagt attgcatttt ctttgttgct agtatcagaa
     2341 attcctatgt tttctttaaa atttgcaaca tattcttttt ctgaaaattc atttcgatat
     2401 atatttattg ccctttctat tgttgctttt atagttttta atttattgtc attaccattt
     2461 ataattctag catatattct ttcatctatt gttttatata ttatttcaaa aagaaagtaa
     2521 ttaatggatt ataaacatat attagaacat gatgattggc aacgaatatt tgatgcttta
     2581 ccatttcctg tttatatttt acataaagat tataccttat attttgctaa taaagcaaca
     2641 ctgaaacttg atgaatatgt taacaaacat attttttcat taccctgcta caaagcatca
     2701 caattgcata aaaatgaaat aaatacatgt ctctatagaa ataaggaaca tataacacag
     2761 tcaatttctc acactattca tgtacccgaa atagatacat attttcatat aacctgcagt
     2821 ccattgtttg ataaaacggg taaacttgat aaaataattc atgttgctac cgatattagt
     2881 gaagtttatg aaataaaaaa tcaagcagtt gaacatgaat tacagtataa aaagttattt
     2941 gaaaaccata tttcgggttt tggattacac aaaatattgt gcgatgattc aggaaagcct
     3001 atagattata tgtttttaga agtaaatact gcttttgaac gaatgactgg gcttaaagca
     3061 tctgaaatta ttggtaaaag agtaaaagaa atttttcctc aaatagaaga ttactggatt
     3121 gagaactatg gtagagtagc attaaacggt gaaccattta gttttcaaaa ttattcacaa
     3181 gcccttgata aatattttga agtagtagca tatagcccca agcatatgca ctttgctgtt
     3241 gtgtttaatg atataacaga aaaccataaa aatttacaaa ccataaaaga acaagaagaa
     3301 cggttgcatg caatcataaa tcaagcaccc gatgctgtat atttatcaga tatgcaaggg
     3361 cggtttatag ttgtaaatca aacagcatgt gaagaaaatg gttatactat tgatgaaatg
     3421 ctcaacatgt cagtttttga tatagatatt aatataattt caaaaaatga aattatgtct
     3481 atatgggaaa ctatgccctt gcatacgtca caaacattaa taactaagca taaaagaaaa
     3541 aattcgacta cctatccggt tgaaatacga atagcaaaaa ttttgctcga taataaacct
     3601 catattattg gttttgcaca aaatgttaca caaagagtta cgcaagaaag agagttacaa
     3661 ctgcacaatg cacgtttaga gagtttatat gctatttcgc aacatcaagc caatgaaaag
     3721 gctgtgtttc tggattttgt attagatgag gtaattaaac ttactgaaag tagtattggt
     3781 tatatttatt attacaatga agatacagag ttatttactc ttaattcgtg gtcaaaagat
     3841 gtattagatg cgtgtactat tgttgaaaaa caaataacat atgaattaga aaaaactgga
     3901 atttggggag aagtagtaag gcaacgaaaa ccaattatca ttaatgattt tgcagctcag
     3961 aatctattaa aaaaagggta tcctgaaggt catgttcatc taagttcatt tgcttcggca
     4021 cctattattg ttgataataa aattgtggca gtagtaggag tagcaaataa aactacagac
     4081 tatacacaaa tagatataat gcacatgcaa atagtaatgg attctgcatg gaaaatagtt
     4141 gaaagacata ctttgtttga agaacttgta aaagcaaaag aaatagccga agaaaataat
     4201 gcgcttaaaa cagctttttt ggcaaatatt agccacgaaa ttagaacgcc aatgaatgca
     4261 atcctcggtt tttcagaatt actgtcggcc gataatcaat ctatagaaga acgtgaatat
     4321 tttatttcga ttataaaaca atccggtaag caattattaa gagtaataaa tgatataata
     4381 gatatagcaa aaatatcggc aaatgctata aagcctgata tgcgaatatg tgatgtgtta
     4441 catataataa acaattcaat agaaattgtt aagcaacagc aatacatgaa attacaaaac
     4501 aaaatacaga tacatattca agagcatttt ccacaatata taattcaatc agatccggtt
     4561 cgtttgcagc aaatttttac taatcttata agtaatgcgg ttaagtacac tccacaaggt
     4621 actgtagaaa ttggttcaat accatataca tcagattata tttgtttgta tgtaaaagat
     4681 accggtattg gtattccaaa agaatttcat gaaaaaatat ttgaattgtt tcgtcaagta
     4741 catgcaaatc aaaccataga aggaaccgga ataggattaa atatcacgca aggacttata
     4801 catttattag gtggcagtat ttggttagaa tcagtagaag gtaagggtac aacatttttt
     4861 ataaccattc caaatacaca aatagaaaat gtaattgata ctacatcaaa agtttcttta
     4921 tattctattc ctgattggtc aacaaaaact atttgtatag ccgaagataa tattcattct
     4981 tataaattac tcgaaattat cttagcacca actcaaatca gtattttata tgctaaaaat
     5041 ggtgcagaat tacttgaaat atacgcagct aaatctcaca atatagattt aatattactt
     5101 gatattcata tgccagtaat gaatggtgtg gaaac
//
LOCUS       DAOTOW010000057         5084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13637_length_5084_cov_2.682442, whole genome shotgun sequence.
ACCESSION   DAOTOW010000057 DAOTOW010000000
VERSION     DAOTOW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13637_length_5084_cov_2.682442"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1124)
                     /gene="hisC"
                     /locus_tag="P5243_01600"
     CDS             complement(57..1124)
                     /gene="hisC"
                     /locus_tag="P5243_01600"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRS18170.1"
                     /translation="MKNTFSISHLLRKNIQQIKPYSTARDEYSGVALINLDANENPFG
                     SIDGSLYNRYPDPYQIELKKRISQIKNVPVENMFLGNGSDEGIDLLYTAFCNPGVDNV
                     ILCPPTYGMYKVSADTNDILCKEVALTEDFQLQVSKILDSIDEYTKIIWLCSPNNPTA
                     NLLRKSDIQYIVEKTNCLVVIDEAYIDFASEPSWIHTLHDYSNVVVLQTFSKAWGLAN
                     IRLGMLFASQEIIAVLNKIKLPYNISGLVQEYALKVLSGNLEAKDIMVEQIIQEREKM
                     AETLTKVNGVRKVYPSDANFLLVKIDNAHQVYLFLVDKGIIVRDRSKIVLCEDSLRIT
                     IGTPQENTMLIAQLREFNTKR"
     gene            complement(1149..2432)
                     /gene="hisD"
                     /locus_tag="P5243_01605"
     CDS             complement(1149..2432)
                     /gene="hisD"
                     /locus_tag="P5243_01605"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRS18171.1"
                     /translation="MEILVNPSKQEWKSALQRPLIENKQLETTVQEIMEQVKNNGDAA
                     VLRYSQQFDNPNVNKLEVSFAEIEAAQHSISPDLKHAIVTAIKNIQTFHERQEEIPSV
                     TETMYGVKCWRKSVPIEKVGLYIPGGSAPLFSTILMLAIPAKIAGCKEIIVCTPESKQ
                     GGIHPAILYAAQQAGVTKIFRIGGAQAIAAMAYGTETIPQVYKIFGPGNQFVTKAKMM
                     AAMQGIAIDMPAGPSEVMVIADDTAEPAFIAADLLSQCEHGADSQSILVTNSMTIAHK
                     TKEEVDKQLSQLTRKELASSSLQHSKIIVFDDMNHAVELMNEYAPEHLILSVQAPFQF
                     SKKVINAGSVFLGNYSPESAGDYASGTNHTLPTNGYAKAYSGVSLDSFVKKITFQEIT
                     QEGLQTLGPIIETMAAAEMLDAHKNAVRIRLNSIS"
     gene            complement(2512..3447)
                     /locus_tag="P5243_01610"
     CDS             complement(2512..3447)
                     /locus_tag="P5243_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HRS18172.1"
                     /translation="MILEVTTSSVFLIGLSILAFFILVNSIKVVPQREAYIIERLGKY
                     HETLIAGFKIIVPFIDKVAYKHSLKEQAIDVASQTCITRDNIAVEVDGILYLQVIDPQ
                     KASYGIDNYIFAAIQMAQTTMRSVIGKLELDKTFEERDSINSVIVDAVDKASDPWGVK
                     VTRYEVKNITPPQSIKDAMEKQMRAEREKRAIIAESEGAKQAKINVAEGDKQELIARS
                     EGEMQKRINEAKGKAAEIEAIATATANGLTQIAKAIGSEHGIDAVNLRIAEQYLGEFG
                     KLAKTTNSMIIPQNMNDISSMLATAMSVIDHSKKK"
     gene            complement(3462..3905)
                     /locus_tag="P5243_01615"
     CDS             complement(3462..3905)
                     /locus_tag="P5243_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRS18173.1"
                     /translation="MELSLIENPAVVWILFGIAGLLLELVLPGLIILFFGFGALLTGF
                     VYWIIPLGIEFQLIIFLVSSSVLLILFRKWLQKSFFEKRAENKDELEEEFVGKQAEAV
                     TDFVAGSGKVSFKGTTWNAVSTQTIKQGEKVIIEKRDSLTLIVTL"
     gene            complement(3982..4845)
                     /gene="hisG"
                     /locus_tag="P5243_01620"
     CDS             complement(3982..4845)
                     /gene="hisG"
                     /locus_tag="P5243_01620"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRS18174.1"
                     /translation="MEQTVIKLAIQKSGRLSEKSIELLKNCGIKFVSNSSSLKTKAFN
                     FPMEILFLRDDDIPRYVADGVADFGILGMNEIVEQNQNVEVIKKLGFSKCRLSIAVPN
                     DFKYDSIQSLEGKKIATSYPVLLEKHLNSVGVKAEIHEISGSVEIAPTIGLADAICDL
                     VSTGGTLLMNGLKEVEEIYKSEAALIGNVNLSSEKKALLAKWMLRIEAVEKAQNNKYI
                     LMNVPNSAIEEVCNLLPGMKSPTIIPLKTEGWSSVHSVIHEDTFWEIIEKVKAAGAEG
                     ILVCPIEQMVL"
BASE COUNT         1586 a          924 c          686 g         1888 t
ORIGIN      
        1 attttaattg ttctcaattt agaacgatat atactgctct atacttacaa tgtgtattaa
       61 cgtttagtat taaattcgcg taattgagct attaacatgg tattttcttg tggagtacca
      121 attgtaattc gcaaactatc ttcgcacaaa actatttttg aacgatctct tacaattata
      181 cctttatcaa ccaaaaacaa atacacttga tgagcattat caattttcac aagtaaaaaa
      241 ttagcatcac tcggataaac ttttctcact ccatttactt ttgtgagagt ttctgccatt
      301 ttctctctct cttgaattat ttgctcaacc attatatctt ttgcttcaag atttccggac
      361 aacaccttca atgcatattc ttgtacaagt ccacttatat tatacggtag ttttatttta
      421 tttaacacag ctataatttc ttgtgacgca aacagcattc ctaatctgat attagctaat
      481 ccccatgctt ttgaaaaagt ttgcaacaca actacatttg aataatcatg caatgtatgt
      541 atccatgagg gttctgaagc aaaatcaata tatgcttcgt caataacaac caaacaattt
      601 gtcttttcaa caatatattg aatatcagat tttcgcaata agtttgcagt tggattattt
      661 ggtgaacaca accatataat ctttgtatat tcatcaatgc tatctaaaat tttagaaact
      721 tgtaattgaa aatcttccgt caacgcaacc tccttgcata atatatcatt tgtatcggca
      781 gaaaccttat acataccata tgttggcggg cataatatga cattatcaac tcccggatta
      841 caaaatgctg tatataataa atcaatacct tcatcgctgc cattacctaa aaacatattt
      901 tcaacaggta cattttttat ttgagaaata cgttttttta attcaatttg atatggatcc
      961 gggtatctat tgtacaaact accatcaata gatccaaatg gattctcatt ggcatctaaa
     1021 ttaatcaaag caacacctga atattcatct cgagctgtag aatagggctt tatttgctgt
     1081 atattttttc gtaataaatg tgaaattgaa aaagtatttt tcataaaata tttcagtatt
     1141 agacagtatt aagaaattga atttaatctg atacgaacgg cattcttatg ggcatcgagc
     1201 atttctgctg cagccattgt ttctataata ggcccgagag tttgcaatcc ttcttgagta
     1261 atttcttgaa atgtaatttt tttcacaaaa ctatcgagcg aaaccccact ataagcttta
     1321 gcatatccat ttgttggtaa tgtatgattt gttcctgatg cataatcacc tgcactttca
     1381 ggtgaataat ttccaagaaa tacagaacct gcattaataa cttttttact aaattgaaat
     1441 ggtgcttgta cagataatat taaatgttcc ggtgcatatt cattcattag ctcaactgca
     1501 tggttcatat cgtcaaaaac tattattttg ctatgttgta atgatgaaga cgctaattct
     1561 ttgcgagtta attgagaaag ttgtttgtca acctcttctt ttgttttatg agctattgtc
     1621 atagaattag taaccaaaat agattgacta tcggcaccat gttcacattg cgataataag
     1681 tcggcagcaa taaaagcagg ttcagcagta tcgtcagcaa ttaccataac ttcagatggt
     1741 cctgctggca tatctatagc aattccctgc atagctgcca tcatttttgc ttttgttaca
     1801 aactgattac ccggaccaaa aattttatat acttgtggta tagtttctgt accataagcc
     1861 attgctgcta tagcctgagc acctccaata cgaaatattt tagttactcc tgcttgttga
     1921 gctgcatata gtatagcagg atgtattcct ccttgttttg attcgggtgt acatacaatt
     1981 atttctttgc aaccggctat ttttgcaggt atagcaagca tcaaaatagt agaaaataaa
     2041 ggtgctgaac ctccgggtat atataacccg actttttcta ttggtacact tttacgccaa
     2101 cattttacac catacatagt ttctgtaacc gatggtattt cttcttgtcg ttcatgaaac
     2161 gtttgtatat ttttaattgc ggttacaatt gcatgtttta aatcgggtga aatagaatgt
     2221 tgagctgctt caatttcagc aaatgaaact tcaagtttat tgacatttgg attgtcaaac
     2281 tgttgagagt aacgcaaaac agctgcatct ccattgtttt taacttgttc cataatttct
     2341 tgaacagttg tttctaattg tttattttca atcaatggtc gttgtaacgc cgatttccat
     2401 tcttgctttg aaggatttac aagtatttcc atagtatagt aatttgttac aaatatataa
     2461 aaaaagccat accctataga ggatatggct cttttaggtt ttttttaata attatttctt
     2521 tttactatga tcaataacag acatagctgt tgccaacatg ctacttatat cattcatatt
     2581 ttgaggaata atcatagaat ttgttgtttt tgctaatttt ccaaattctc ctaaatactg
     2641 ttctgcaatt ctaagattta cagcatcaat accatgttct gaaccaatcg ctttagctat
     2701 ttgagttaat ccgtttgctg tagctgtagc aattgcttca atttcagcag cttttccttt
     2761 agcttcatta attcgttttt gcatttcacc ttcagaacga gcaatcaatt cttgtttatc
     2821 tccttcagct acatttattt ttgcttgttt tgccccttct gattcagcta taattgcacg
     2881 tttttcgcgt tctgcacgca tttgtttctc cattgcatct tttatacttt gcggcggagt
     2941 tatatttttc acttcatagc gagttacttt tactccccat ggatcacttg ctttatcaac
     3001 agcatcaaca attactgaat ttattgaatc acgctcctca aaagttttat caagttctaa
     3061 ttttccaata acactcctca tggttgtttg agccatttga attgctgcaa aaatataatt
     3121 atcaatacca tacgatgctt tttgtggatc aattacctgt aaatacaata taccgtcaac
     3181 ttctactgca atattatcac gtgtaataca ggtttgagaa gcaacatcaa ttgcttgttc
     3241 tttgagtgaa tgtttatatg caactttatc aataaatggt actataattt taaaaccggc
     3301 tattaaggtt tcatgatact ttcctaaacg ttcaatgata tatgcttcac gttgcggaac
     3361 aacttttatt gaatttacaa gtataaaaaa tgctaaaata cttaatccaa ttaaaaacac
     3421 acttgaggtt gttacttcta aaatcatggg tttatctcct attataatgt tactatgagt
     3481 gttaaactat cacgtttttc aattattact ttttcacctt gttttattgt ttgagtagaa
     3541 acagcattcc atgttgttcc tttaaaactt accttacctg aacctgctac aaaatcagta
     3601 acagcttcag cttgtttacc aacaaattct tcttcaagtt catctttgtt ttcagctctt
     3661 ttttcaaaaa aacttttttg taaccacttt cgaaacaata tcaacaaaac tgacgatgaa
     3721 acaagaaaaa taatcaattg aaattcaata cctaaaggaa ttatccagta tacaaaacct
     3781 gtaagtaatg ctccaaaacc aaaaaataaa attatcagtc ccggtaatac taattccaaa
     3841 agtaaacctg caataccaaa cagtatccaa acaacggcag gattttcaat taaagataat
     3901 tccattttgg tatgttttta tgtaaaaata aagatatatt ttgaatgtaa cttataatat
     3961 atcaattttt tattattttt tttataatac catttgttca ataggacaca ccaaaatccc
     4021 ttcagcacct gcagccttta ctttctcaat aatttcccaa aatgtatctt catgaattac
     4081 agaatgaaca gaactccacc cttctgtttt taatggtatt atagttgggc ttttcattcc
     4141 cggcaataaa ttacaaacct cttctattgc agaattaggt acgttcataa gaatatattt
     4201 attgttttgt gctttttcaa ctgcttctat tcgcaacatc cattttgcaa gcaatgcttt
     4261 cttttctgaa ctaagattta cgttacctat aagagcagct tctgatttat atatttcttc
     4321 tacttctttt aatccgttca tgagcaacgt gccgccggta cttactaagt cgcaaatagc
     4381 atcggccaaa ccaatagttg gagctatttc aacagaaccg cttatttcat gtatttctgc
     4441 ttttactcct acagaattaa gatgtttttc caacaatacc gggtatgacg ttgctatttt
     4501 tttaccctca agcgattgta tagaatcata tttaaaatca ttgggtacag caatagacaa
     4561 tcgacatttt gaaaaaccaa gttttttaat cacttctacg ttttgatttt gttctacaat
     4621 ttcattcatg ccaagtatac caaaatcagc aacaccatcg gcaacatacc ttggaatatc
     4681 atcgtcacgc aaaaacaata tttccattgg aaaattaaaa gcttttgttt ttaaactgct
     4741 tgaattcgaa acgaatttaa taccgcaatt tttgagtaat tcaatagatt tttcacttaa
     4801 tctaccactt ttttgaattg ctaatttaat tactgtttgt tccatattat aaaattaatg
     4861 tatactataa aaaaaaaggc ttgcccgaag caaaccttta tatattaatg cattcagtca
     4921 tgttttgata gtttattata tatgatgatg aatgtgtttc attattttct tttttttgca
     4981 attatacata ttttttattt caaaaaaaaa tcaatatcta tttaatggta aattcatttt
     5041 ttatttttaa gtaattcctg caaggcatac atttcatctc gtaa
//
LOCUS       DAOTOW010000058         5081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13650_length_5081_cov_3.284719, whole genome shotgun sequence.
ACCESSION   DAOTOW010000058 DAOTOW010000000
VERSION     DAOTOW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13650_length_5081_cov_3.284719"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="P5243_01625"
     CDS             complement(<1..546)
                     /locus_tag="P5243_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRS18175.1"
                     /translation="MAEDLMKFDLPQRRAASIKVIGVGGGGSNAVNHMYHQGIKDVDF
                     IVCNTDVQDLQKSPIPTKIQLGDDLTGGRGAGSNPEVGERAAKESMAQIQALLEDDIQ
                     MVFITAGMGGGTGTGAAPIIAEIAKEMGILTVGIVTIPFSFEGKMRYQHAIRGIEALS
                     QNVDSMLIINNETLREKYGNLK"
     gene            complement(573..1964)
                     /gene="ftsA"
                     /locus_tag="P5243_01630"
     CDS             complement(573..1964)
                     /gene="ftsA"
                     /locus_tag="P5243_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRS18176.1"
                     /translation="MEQTISNKPMIDRRKAFSAIDIGTTKIVALVGYVNEKNELTVLG
                     RGVAPSMGVSRGEVINIEYTVSSIKAAVKQAEEEAGFFCKKVVVGIAGKHIRTLQNRG
                     GINRENPDKEITNEELEILMQENRHVCLEADEEIIHAIPQTYIVDNNHEVTCPLGMHG
                     RRIEANFHIVVGKGTAIKYIRTAIQRAGLEVSELLLEPLASAKSTLTEEEKRLGVAIL
                     DIGGGTTDVAIFHNNVVQFTEVIPFGGNAITHDISVEYNILEELAEKLKVEYGCAIQD
                     VVKDNEIVSIPGVHGRPPKEISFKVLAGIIQARMEEILDAALFVIKTSGYGKYLSQGI
                     VVTGGGSLMQHLTQLINFKINKDAKIGTPIHCVNYMNAREFSNPIYATGVGLLMRAAE
                     LHKAQNVHVEVENTIHNQTKEQVQPAAVKQTATPKVEEHKKEKNNYIYKLVEKFFGDT
                     ATDTKLSETGVGK"
     gene            complement(1977..2753)
                     /locus_tag="P5243_01635"
     CDS             complement(1977..2753)
                     /locus_tag="P5243_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18177.1"
                     /translation="MKKILPYIPLIVLLIIASVSVGFSESYRNELVCKDVLFAIDEKT
                     DKPKLLTESDILGGIKKTKKDFIGKKINSIPLHDIEDAISNNPSVLACECFVTIDGIL
                     HIKIQQRIPVVRIVGLHDNYYIDEHGRLMPLSQNYTARTLVASGNIPEGFRKSNNVLL
                     QTQKDSSSLKDIFLITMAIRKNQFLNSLIEQIYVHNKSQYILISKIGPAIIEFGAIDE
                     YEKKLQNLQAFYMSQKVRENWHLYEKISVKYSNQIVCTKY"
     gene            complement(2750..4150)
                     /gene="murC"
                     /locus_tag="P5243_01640"
     CDS             complement(2750..4150)
                     /gene="murC"
                     /locus_tag="P5243_01640"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRS18178.1"
                     /translation="MNNTKYTYYYFLGIGGIGMSALARYFNFKGAQVVGYDRTPSELT
                     HKLESEGIAITYNHQIEYIPKQVIENKEKTLVVYTPAIPNDMPQFVHLQSLGYTCVKR
                     AYVLGLICKNQHGIAISGTHGKTSVTTFTSHILYNSSKGCNAFLGGISKNFKSNYVFH
                     PTSTNVVIEADEYDRSFHSLYPSTALVTSMDADHLDIYGSHTAILVSFKQFFSQIQPG
                     GTFIYKKNLDVSESLPMLQEKNIQCYSYSINDIRADFYARVITRKHNAYSIDLVTPFG
                     EISNIIFQLPGKVNIENMIAASAVALCNGVQTNELVQALFTLQGVERRLDIRVSNSKV
                     LYIDDYAHHPEELKACITSLKELYSTAKITGIFQPHLYSRTRDFAKEFGQSLSLLDEL
                     LLLDIYAARELPIEGVTSQLIFDAVTIPNKRIVSKHELLQYIEHNVPEVLVTMGAGDI
                     DRFVKPLEDLLKRITQ"
     gene            complement(4151..>5081)
                     /gene="murG"
                     /locus_tag="P5243_01645"
     CDS             complement(4151..>5081)
                     /gene="murG"
                     /locus_tag="P5243_01645"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRS18179.1"
                     /translation="IIGLPISGFERKLSLYNVKTMCNIVKSLHKARKIIKDFKPDIAV
                     GVGGYASGPMLYAAQKHGIPTIIQEQNSYAGVTNKLLAKRVKKVCVAYDGMERFFNPQ
                     TIVFTGNPVRPAIQNMNCSVQESLDFFGLQAHVPTILIVGGSLGARTINHSVAANIQT
                     IAKAGVQVIWQTGKTYYEEAKRIAQSYPNIKVFDFIKQMDYAYTAATIIISRAGAGTI
                     SELCIVGKPCVFVPSPNVTEDHQTKNAQALVAKQAAVMIPDSEAIQKLFDVTLALLNN
                     TQKMNELAAQIKQLAIPNAAEKIVDEILKIVTK"
BASE COUNT         1557 a          957 c          727 g         1840 t
ORIGIN      
        1 ttttaaatta ccatattttt ctcgcaatgt ttcattgttt ataataagca tagaatctac
       61 attttgactc aatgcttcaa taccacgaat ggcatgttga taccgcattt ttccttcaaa
      121 agaaaacgga atagtaacta taccaacggt aagtattccc atttcttttg ctatttctgc
      181 aattataggt gcagctcctg taccggtacc tccacccatt ccggcagtta taaacaccat
      241 ttgaatatca tcttcgagca aagcttgtat ttgagccata ctttcttttg cagcacgctc
      301 tccaacttcc ggattactac cagcacctct tccaccggtt aggtcatcac ctaattgaat
      361 tttagtaggt atagggcttt tttgtaaatc ttgaacatcg gtattacaaa ctataaaatc
      421 aacatctttt attccttgat ggtacatgtg gtttacagca ttactaccac cacctcctac
      481 accaattacc ttgattgatg cagctctgcg ttgaggcaaa tcgaatttca ttaaatcttc
      541 ggccataact gtatggtttt tctgttgata tattatttgc caacgccggt ttccgataat
      601 ttagtatcgg ttgccgtatc gccaaagaat ttctcaacaa gtttatatat ataattattt
      661 ttttcttttt tatgctcttc tacttttggt gtagcagttt gtttaacagc agcaggttgt
      721 acttgttctt ttgtttgatt atgaattgta ttttcaacct caacatgaac attttgtgcc
      781 ttatgaagtt ctgctgcacg catgagtaac ccaacaccgg ttgcatatat tggattacta
      841 aattcacgag cattcatata attaacacaa tgaataggag ttcctatttt agcatcttta
      901 ttaattttaa aatttattaa ttgagttaaa tgctgcataa gagaacctcc acctgtaacc
      961 actatacctt ggcttaaata tttaccatat cccgatgttt taataacaaa taatgccgca
     1021 tcaagaattt cttccattcg agcttgaata attcctgcca atactttaaa agaaatttct
     1081 ttaggtggac gaccatgtac acccggaata cttactattt cgttatcttt tactacatct
     1141 tgtatagcac aaccatattc cactttaagt ttttcggcta attcttcaag aatattatat
     1201 tctacactta tatcatgtgt tattgcatta ccaccaaatg gaataacttc agtaaattgc
     1261 actacattat tatgaaaaat agcaacatcg gtagttcctc caccaatatc aagaatagca
     1321 acacccaatc gtttttcctc ttcagtaaga gtagattttg ccgaagcaag aggctccaat
     1381 aataattcac ttacttctaa tcctgcacgt tgaattgctg ttcgtatata tttaattgct
     1441 gttcctttac caacaactat atgaaaatta gcctctattc ttctaccatg catgccaagc
     1501 ggacaggtaa cttcatgatt attgtcaaca atataagttt gaggaatggc atgtattatt
     1561 tcttcatcgg cttctaaaca tacatgtcga ttttcttgca taagtatttc taattcttca
     1621 ttagtaattt ccttatctgg attttctcta tttattcctc cacgattttg caatgttcgt
     1681 atatgttttc cggctatacc taccactacc tttttgcaga aaaaacctgc ttcttcttcg
     1741 gcttgtttta cggcagcttt aattgaactt actgtatact caatattaat aacctcaccg
     1801 cgacttactc ccatagatgg tgccacgcca cgtcccaata cagttaattc atttttttca
     1861 tttacatagc caaccaaagc tacaatttta gtagttccaa tatcgattgc cgaaaacgcc
     1921 ttgcgtctat caatcattgg tttgtttgaa attgtttgtt ccatactttt ttatatttaa
     1981 tattttgtgc atacaatttg attactatat ttcacgctaa ttttttcata caaatgccaa
     2041 ttttcgcgta ctttttgcga catataaaaa gcttgcaggt tttgtaattt tttctcatat
     2101 tcatctattg ccccaaattc tattatagca ggacctattt tgcttatgag tatatattgt
     2161 gatttattgt gaacatatat ttgttctatc aaagaattca aaaattgatt cttacgaata
     2221 gccatagtta taagaaaaat atcttttagt gaactagaat ctttttgtgt ttgcaaaagc
     2281 acattgtttg attttctgaa tccttccgga atattacccg aagcaactaa tgtgcgagca
     2341 gtatagtttt gtgacaatgg cattaatctt ccatgttcat ctatataata attatcatgc
     2401 aatcccacta ttcgcacaac aggaatacgt tgttgaattt taatatgaag aattccatca
     2461 attgtaacaa aacattcgca tgcaagcacc gatggattat ttgaaatagc gtcttctata
     2521 tcatgcaatg gaattgaatt tatttttttt cctataaaat ctttttttgt cttttttatt
     2581 cctcctaaaa tatccgattc ggttaataat ttaggtttat cagttttttc atctattgca
     2641 aacaatacat ctttacacac caattcatta cgatacgatt ctgaaaaccc tacgcttact
     2701 gatgctataa tcagcaaaac aatcaatggt atatatggaa gtattttttt cattgcgtaa
     2761 tacgtttcaa taaatcttct aaaggtttta caaatctgtc aatatctccg gctcccatag
     2821 tcacaagcac ttcaggaaca ttatgctcaa tatattgtaa cagctcatgt ttacttacta
     2881 ttcgtttatt aggaattgtt acagcatcaa aaattaattg tgaagtaact ccttctattg
     2941 gtaattctcg agctgcatat atgtcaagca acaataattc atcaagcaat gaaagacttt
     3001 ggccaaattc ttttgcaaaa tctcgagtac gagaatataa atgaggctga aaaattccgg
     3061 tgatttttgc tgttgaatat aattctttaa gagacgtaat gcatgctttc aattcttcgg
     3121 gatgatgagc ataatcatca atataaagta ctttagaatt agaaacacgt atatcgagtc
     3181 tacgttctac cccctgtaac gtaaacaatg cttgaactaa ttcatttgtt tgtaccccgt
     3241 tgcaaagtgc aacagcagaa gctgcaatca tattttctat attaacttta cccggcaatt
     3301 gaaaaataat attagatatt tctccaaaag gtgtaactaa atctattgaa taagcattgt
     3361 gtttacgagt aataacacgt gcataaaagt ctgctctaat atcattgatt gaatatgaat
     3421 aacattgaat atttttttct tgtaacatag gtaaactttc agatacgtca agattctttt
     3481 tataaataaa tgttcctccc ggttgaattt gagaaaaaaa ctgcttaaac gaaaccaata
     3541 tagctgtatg agaaccatat atatctaaat ggtcggcatc cattgatgtt actaatgccg
     3601 tagaaggata aagactatga aaagaacgat catattcatc agcctctatt accacatttg
     3661 tacttgtagg atgaaacaca taattacttt tgaaattttt tgaaataccg cctaagaatg
     3721 cattacaacc tttacttgaa ttatataata tatgcgatgt aaatgtagta accgatgttt
     3781 tgccatgagt tcctgaaatt gcaattccat gttgattttt acaaataagt cctaacacat
     3841 acgctctttt tacacaagta tatcccagac tttgaagatg cacaaactgt ggcatatcat
     3901 taggaatggc aggtgtgtac accacaagcg ttttttcctt attttcaatt acttgttttg
     3961 gaatatattc tatttggtga ttatatgtta ttgctattcc ttctgattca agtttatgag
     4021 ttaattcaga aggagtacga tcatatccta ctacttgagc tcctttaaaa ttaaaatatc
     4081 gagccaaagc actcatgcca atacctccaa tgcccaaaaa gtaataatat gtatactttg
     4141 tattattcat ttattttgta actattttta gtatttcatc aacaattttt tcggcagcat
     4201 ttggtattgc caattgcttt atttgcgctg ctaattcatt cattttttga gtattattaa
     4261 gtaatgcaag tgttacatca aataattttt gaatagcttc agaatccggt atcataactg
     4321 ctgcttgttt agcaacaaga gcttgtgcat ttttggtttg atgatcttcg gttacatttg
     4381 gtgaaggaac aaacacacag ggttttccaa ctatacacaa ctcagaaata gtacctgctc
     4441 cggctcgcga aattattata gtggcagcag tataggcata atccatttgt ttaataaaat
     4501 caaacacttt gatattagga tatgattgag ctatacgttt agcttcttca taatatgttt
     4561 ttccggtttg ccaaattact tgtactccgg cctttgcaat tgtttgaatg tttgctgcta
     4621 cactatgatt aattgtacgt gcacctaaac ttcctcctac aatcaatata gttggcacat
     4681 gggcttgcaa tccaaaaaaa tctaaagatt cttgtacact acaattcata ttttgtatag
     4741 caggtcgcac cgggttaccg gtaaacacaa tagtttgcgg attaaaaaaa cgttccatac
     4801 catcatatgc tacacaaact tttttaactc tttttgccaa caatttattg gtaacacctg
     4861 catacgaatt ttgttcttga attatagtag gtattccatg tttttgagca gcatataaca
     4921 ttggtccact tgcataacca ccaacaccca cagcaatatc tggtttaaaa tctttaataa
     4981 ttttgcgagc cttgtgtaaa ctttttacta tgttacacat tgtttttaca ttgtataaag
     5041 aaagttttcg ctcaaaacca ctaattggta gcccaataat a
//
LOCUS       DAOTOW010000059         5071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13680_length_5071_cov_3.964514, whole genome shotgun sequence.
ACCESSION   DAOTOW010000059 DAOTOW010000000
VERSION     DAOTOW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13680_length_5071_cov_3.964514"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..963
                     /locus_tag="P5243_01650"
     CDS             160..963
                     /locus_tag="P5243_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18180.1"
                     /translation="MKIKFLVFILSIFFSNTAQCDIIFEYTHNVDFCAKIVNIEDFPN
                     LSLIAKVSGPVSPSTYVIKQTDCLSIGYKMNSIKVYACESWFVKYYDLNKVNFANNPN
                     VLESSIPIKAYYGDVNNSNPINRIQYFYKIAGFTETTVEIYLWKEIISYNNGADDKIN
                     YYEYEGNTTLLKQNISTGLPTKQIYSNIQITPNPAKSFCDVSINTLYQGEVLIAVSNI
                     SGNQIAAYSFQKSNYCFRERLYLGTLSKGTYFITIQQGNAVKTEKLIIQ"
     gene            973..1323
                     /locus_tag="P5243_01655"
     CDS             973..1323
                     /locus_tag="P5243_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18181.1"
                     /translation="MKYEISFLYALLFTIIIETSVIWILCKTTYKTINLSNISILLSG
                     FILSFATLPYVWFVFPYFIHQRFLYISVSEMFAFVTEAILLWSIFKVPIKHSFVLSFC
                     ANICSYIIGLLLVF"
     gene            1361..2386
                     /locus_tag="P5243_01660"
     CDS             1361..2386
                     /locus_tag="P5243_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18182.1"
                     /translation="MRKFICNTIPGFVTTYSFCHALIDCACIFFVLGVIQYSSEIILL
                     YNVLAFGLQLPFGIIFDKLYIPQKMAILGLSLVLISFFLIITPYIAVVIIGIGNALFH
                     IGGGGGILQMQKYTTRYSGIFVAPGGVGLAIGMFLSKISNYYIYYILIGILCLSIGML
                     FFVQTPQRNKIQNKIPISFVLIGTIFLIWVTIIIRSFVSTHLHFEWKSDTNWYIVFVL
                     AVALGKILGGFLSEILGFIKVGVGGLIVSLLLFCLGNTFFLCGILGFVFFNFTMTVTL
                     ISILSLMSGKAGVSFGLTTVALLLGNYIPMNWVDSHVLMFVSIFISVILLYVGLILRN
                     KIISKFP"
     gene            2466..4409
                     /locus_tag="P5243_01665"
     CDS             2466..4409
                     /locus_tag="P5243_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRS18183.1"
                     /translation="MSQNSGKLFEEFAPVSYEQWKEKTIADIKGADFDKRMVWKPIDG
                     FSVEPLYRLEDLEPVKYLDVLPGQFPYTRGTKKTNNDWYIRQNITVTNPAQGNKKALE
                     VLQKGITSLGFEIEDTNNYSAQNVASLLQDIDLTAIETCFVIGRGKTAFFKLFVAYVQ
                     SKGYDVAKIEGSINSDYIGNFVKKGAFCYDSQEVCENNISELITIAKALPKFRVCEVH
                     ADYISNAGGSITQELACAMAMGAEYLTIVTNKGIDAGIFAPKMKFNFAVTSNYFMEIA
                     KFRAAKLLWAKIVEAYNPKCDCNEFCSCEEELGTCGCCTEKICTCAGKINIHAITSTW
                     NKTLYDPYVNMLRTQTEAMSATIGGVDSLTVLPFNYVYEDPTDFSERIARNQQSLLKE
                     ECHFDAIVDPAAGSYYIEQLTDKIAENSWNLFLQIQDKGGFIEAFKAGFIQDFVKATA
                     AAHNKNIATRRENVLGVNQFPNFTEVIQNDVCECVFKPEFEKSDKAIAEPLAMYRGAQ
                     AFEALRYKTDVYAKTNARPKVFMLTIGNLTFRKARAQFACNFFACAGFEVIDNNGFAT
                     IDEGVKAAKDSGAHVVVICSSDEEYADVAVPIFEKLQDKIVVVAGAPACTDDLVAKGI
                     KNFISVKSNVLETLQSYQSMLGI"
     gene            4428..>5071
                     /locus_tag="P5243_01670"
     CDS             4428..>5071
                     /locus_tag="P5243_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRS18184.1"
                     /translation="MKPDFKSIHIKTSKAGTCNVQEWEKKNNISANWLTPEQIPVKPV
                     YTKEDLKGMEHLHFTSGFAPYLRGPYTAMYPYQPWTIRQYAGFSTAEESNAFYRRNIA
                     SGQKGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDSIMDMRILFDQIPLNKMSVSI
                     TMNGAVLPVMAFYILTGLEQGAKLEELAGTIQNDILKEFMVRNTYIYPPAFSMK"
BASE COUNT         1712 a          772 c          865 g         1722 t
ORIGIN      
        1 aaattttgtt ttattaaatt aattttgtag ttttatcgga caataatgaa aaaaaaatac
       61 ttatacaata tttttataat atttttcttg tgtttcacaa aaaatcaata tctttgaatg
      121 atgttattta aaataaacat cataaataac tctcattata tgaaaataaa atttctggta
      181 tttattttaa gtatattctt ttctaatact gcacaatgtg acataatatt tgaatataca
      241 cataacgttg atttttgtgc aaaaatagtt aacattgagg attttccgaa tctttcttta
      301 attgcaaaag tatcaggacc cgtttctccc agcacatatg taattaaaca aaccgattgt
      361 ttaagcatag gctataaaat gaatagtata aaagtgtatg catgtgaatc atggtttgtc
      421 aaatactatg atttaaataa ggtaaatttt gctaataatc caaatgtatt ggaatcgagt
      481 attcctataa aagcatacta tggcgatgtt aataattcaa atccaataaa tcgaattcaa
      541 tatttttata aaatagcagg gtttaccgaa actactgtag aaatatatct ttggaaagaa
      601 attatttctt ataataatgg tgctgatgat aaaataaatt attatgaata tgaaggaaat
      661 acgacattat taaaacaaaa tatttcgact gggttaccta caaagcaaat atattctaat
      721 attcaaatta cacctaatcc tgcaaaatca ttttgtgatg tttctattaa taccttgtat
      781 caaggtgaag ttttaattgc tgtttcaaat atttctggta atcaaatagc agcatattcg
      841 tttcaaaaat ctaattattg ttttagagaa cgattatatc tcggtacgct ttcaaaagga
      901 acatatttta ttactataca acaaggaaat gctgtaaaga cagaaaagct aataatacag
      961 taatgtagca gtatgaaata tgaaatttct tttttatatg cgttattgtt tactataatt
     1021 attgaaacat ctgtaatatg gattctttgt aaaactactt ataaaacaat taatctttcc
     1081 aatatatcaa ttctattatc aggctttata ttatcgtttg caacacttcc gtatgtttgg
     1141 tttgtttttc catatttcat tcatcaacga tttttgtata tttcagtaag tgaaatgttt
     1201 gcttttgtaa ctgaagcaat tttgttatgg agcatcttta aagttccaat aaaacactct
     1261 tttgtattat cattttgtgc aaatatttgt tcgtatatta ttggtttatt gttggtattt
     1321 tgattaaatg tatttttttg tataaaaatt aaaactacat atgcgaaaat ttatatgcaa
     1381 tacaattccc ggttttgtta ctacatattc cttctgtcat gcattaattg attgtgcatg
     1441 tatatttttt gtgcttggag taatacaata ttccagcgaa attatattgt tgtataatgt
     1501 tttagctttt ggattgcagc taccatttgg aatcattttc gataaattat acattcctca
     1561 aaagatggca attctaggat taagtttagt tctgatttca tttttcttaa ttataacccc
     1621 atatatagct gttgtaatta taggaatagg taatgcctta tttcatatag gaggaggcgg
     1681 tggtattttg caaatgcaaa aatatacgac acgatattca ggtatttttg ttgctcctgg
     1741 tggtgttgga ttggcaatag gtatgttttt atcaaaaatt agtaattatt atatttatta
     1801 tatattgata ggaatcttat gtttaagtat tggaatgctt ttctttgtac aaacaccaca
     1861 acgtaataaa attcaaaaca aaattccaat ttcttttgtc ttaattggaa caatattttt
     1921 aatttgggtt accataatta ttcggtcatt tgtgagtaca catttacatt ttgaatggaa
     1981 atcagatact aattggtata tagtgtttgt tttagctgtt gctttgggaa aaattttggg
     2041 tggctttttg tcagagattc taggttttat taaagttggg gttggaggtt tgattgtatc
     2101 gcttttgctt ttttgtctcg gaaatacatt ttttttatgt ggaatacttg gctttgtgtt
     2161 ttttaatttt accatgacag ttacgttaat ttctatattg tctcttatgt caggaaaagc
     2221 aggagtgtca tttggtttaa caacggttgc cttgttactc gggaattata ttccaatgaa
     2281 ttgggtagat tctcatgtat taatgtttgt ttctatcttt atatccgtaa tactattgta
     2341 tgtaggtctc attctaagaa ataaaataat ttcaaaattc ccctaaaaaa ttaggtctgt
     2401 ttcacaaaaa atctatactt ttgaacaggt aaaaacttac agtattcaat aaaactataa
     2461 aaaacatgtc acagaattcg ggtaaattat ttgaagaatt tgcaccggtt tcctacgaac
     2521 aatggaaaga aaaaaccatt gccgacatca aaggtgccga tttcgataaa cgaatggtat
     2581 ggaaacctat tgatggtttt tcggtggaac cgctctacag attggaagat ttagaacccg
     2641 tgaagtattt agatgtactt cccggacagt tcccttatac acgaggaact aaaaaaacaa
     2701 acaacgattg gtatattcgt cagaatatta ctgttaccaa tcctgctcaa ggcaacaaaa
     2761 aagcactcga agtattgcaa aaaggtatca catctttagg attcgaaata gaagatacca
     2821 ataattattc agcacaaaat gttgcttcat tgcttcaaga tattgacctt acagctattg
     2881 aaacatgctt tgtaataggt cgcgggaaaa cagcgttctt caaattattt gttgcatatg
     2941 tgcaatcaaa aggctatgat gtagcaaaaa tcgaaggctc aattaattca gattatatcg
     3001 gaaattttgt aaaaaaagga gcattttgtt atgattctca agaagtatgt gaaaacaata
     3061 tttctgaatt aataaccata gcaaaagcgc ttcctaaatt ccgtgtatgc gaagttcatg
     3121 cagattatat tagcaacgcc ggcggttcta taacacaaga acttgcatgt gctatggcta
     3181 tgggtgccga atatcttact atagttacca ataaaggaat tgacgcagga atatttgctc
     3241 caaaaatgaa atttaatttt gctgtaacat caaattattt catggaaatt gcaaaatttc
     3301 gtgcagcaaa actattatgg gcaaaaatag ttgaagcata caatccaaaa tgtgattgca
     3361 acgaattttg ttcgtgcgaa gaagaactag gtacatgtgg ctgctgcaca gaaaaaatat
     3421 gtacatgtgc aggaaaaata aacattcatg ccataacttc tacatggaat aaaactttgt
     3481 acgacccata cgtaaatatg ctccgtacac aaaccgaagc tatgagtgct acaataggtg
     3541 gtgtagattc acttaccgta ctaccgttta actatgtata cgaagaccct accgacttta
     3601 gtgaacgtat tgctcgcaat caacaaagtt tattaaaaga agaatgtcac tttgatgcaa
     3661 tagttgaccc tgctgccgga tcatactaca tagaacaatt gaccgataaa attgctgaaa
     3721 attcatggaa tttgtttttg caaattcaag ataaaggtgg ttttatagaa gcatttaaag
     3781 ccggatttat tcaagatttt gtaaaagcta cagctgcagc acacaacaaa aatattgcta
     3841 ctcgtcgcga aaacgtatta ggagttaatc aatttccaaa ctttaccgaa gttatacaaa
     3901 atgacgtttg tgaatgcgta tttaaacctg aatttgaaaa atcagacaaa gctatagcag
     3961 aaccattggc aatgtatcgt ggagcacaag catttgaagc attgcgttac aaaaccgatg
     4021 tatacgcaaa aaccaatgct cgtccaaaag tgtttatgct tactattgga aatcttactt
     4081 tccgtaaagc tcgtgcacaa tttgcttgta acttctttgc atgtgccggc tttgaagtaa
     4141 ttgacaataa tggatttgca acgattgacg agggagtaaa agctgctaaa gattcgggtg
     4201 ctcatgtggt tgtaatttgt agttccgacg aagaatatgc tgacgtagct gttccgattt
     4261 tcgaaaaatt gcaagacaaa atagttgtag ttgcaggtgc acctgcatgt accgacgatc
     4321 ttgtagctaa aggcatcaaa aacttcataa gcgttaaatc aaatgtactt gaaacgctac
     4381 agtcgtatca atcaatgtta ggaatttaat ttttaagatg tactacaatg aaaccggatt
     4441 ttaaatcaat acatattaaa acttccaaag cagggacttg caatgtgcaa gaatgggaga
     4501 aaaaaaataa catctctgcc aactggctaa cacccgagca gataccggtg aagcctgtat
     4561 ataccaaaga agacctcaaa ggcatggaac acctgcattt tacttcgggt tttgctccat
     4621 acttacgcgg tccgtatact gccatgtatc catatcaacc atggactatt cgtcagtatg
     4681 caggattttc aacagccgaa gaatctaacg cattctatcg tcgtaatata gcttcgggtc
     4741 aaaaaggttt atctgtggca tttgaccttg ctacccaccg cggatacgat tctgaccacc
     4801 cacgtgtagt tggtgacgta ggaaaagccg gagtcgctat agacagcatt atggatatgc
     4861 gtatattgtt cgaccaaatt cctctcaaca aaatgtcggt aagtataact atgaacggag
     4921 cagtattacc ggttatggca ttctatatat tgacaggttt ggaacaaggt gcaaaattag
     4981 aagagcttgc aggaactatt caaaatgata ttttaaagga atttatggta cgcaacacct
     5041 acatttatcc gcctgcattt tctatgaaaa t
//
LOCUS       DAOTOW010000060         5065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13698_length_5065_cov_3.400998, whole genome shotgun sequence.
ACCESSION   DAOTOW010000060 DAOTOW010000000
VERSION     DAOTOW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13698_length_5065_cov_3.400998"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="P5243_01675"
     CDS             <1..1531
                     /locus_tag="P5243_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS18185.1"
                     /translation="MVNVYKDDYTILSIQSTVLNTPKNPVCVGDQVRLTAQTSSPTQR
                     YLWSTSPYFFPILNSDFKQNSIVVNPTETTMYYVHTLSMFCEYEDIDSILVEVADNKI
                     IATGDTLICKNDVATISVQNLINGSSLSYNWTPKSAVVSGGDSENAIVRPERSTQFIV
                     YATNYAGCIVQDTVQVNVDEVKIETSVYNPISCHGQTDASIVLSPKGIEPYTYEWENG
                     LNSSQRSQLGEGKYIVTVTDGLGCINTREFVIEEPDLLQIIDTSIWYVTCDAACNGAI
                     DITISGGTLPYAYLWSRGDTTKTIQSLCQGLYNVNVTDAHGCKTSLAQAVIIGKHERL
                     PYLDAFAEQSILFKGQSTQLHASPIPIDTVSYFWYPNIWMENYALPIVTVVPQDSFTY
                     YVRAIDTYGCESVDTVQVTVHEWVCDDPFIYIPTAFTPNNDGKNDMLYIKSYVITDLY
                     FAIYDRWGEKLFETTDITKGWDGTYQGKVLQPQVLVYYLEATCLNQQKFKSKGNITII
                     K"
     gene            1535..2551
                     /locus_tag="P5243_01680"
     CDS             1535..2551
                     /locus_tag="P5243_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRS18186.1"
                     /translation="MKRIIISILYACFVFQAFGQDIHFSNFYTNVLNLNPAFTGYFKG
                     KYRYNLTYRDRYRTVAVPYQTVSLGIDTKTSKLHGLRKTFGYGILLNYDIAGDSQFNT
                     IQLQFPVSYIHITSNKQWNLSAGLMPALVFNSLDYTLLRFPDQFDGVQYNPNLPMNED
                     FDKTNSFFFNIGAGGQATYKPNKTQSYTMGLAGYNLTTPNISFFDDKSVQLYARMLIH
                     AMAIVEVKPDVDMVPAVKMQFQGKQKEYHVGAMAIKYFDNLSIIDVSGGVWFRSRDRD
                     AIIVGVGCNYLGYTILCNYDINISSLKKASNGHGAFELTVSSILYEQKKRKKMTSVKC
                     PPYL"
     gene            complement(2553..3515)
                     /locus_tag="P5243_01685"
     CDS             complement(2553..3515)
                     /locus_tag="P5243_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRS18187.1"
                     /translation="MNKQITVGVDIGGTNTAIGFVDREGNCVFETSIPTKEHVEIEPY
                     IKNLAGIIQQELTQKPEFELVGIGVGAPNANYHRGTIEEAPNLSWKGTVYFCKMLGSY
                     FPNILVTITNDANAAAMGEMIYGAARGIRDFIVITLGTGLGSGIVVNGDLVYGHDGFA
                     GEVGHTTVFPGGRQCGCGKKGCLETYVSATGMVRTMAEILCNSNKPSKLRDIPYNKIT
                     ALDIELALKDGDELAKECFEYTGHILGLKLADAVCHTSPSHIFLFGGVAKAGNLIIEP
                     TKRAMEENLLPIFKNKITIAQSGLLNVNAAVLGASALAWNELEK"
     gene            complement(3624..>5065)
                     /locus_tag="P5243_01690"
     CDS             complement(3624..>5065)
                     /locus_tag="P5243_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRS18188.1"
                     /translation="IIEIRNVIPTVPVLALTATATPDVVADIQNKLGFKRHNVLQTSF
                     ERKNLTYIVKQKEDKLGYILTICKKIRGAGIVYVKTRKQTKEIALALREMGVNTDFYH
                     AGLDAQTRNLKQENWMKSHNMVMISTNAFGMGIDKPNVRFVLHFDMPESLEAYFQEAG
                     RAGRDGLEAFAVLLYNKKDISNLKANFTKKFPDFKYIKNVYEALANYYQIEVGAAKGN
                     VYDFFIDDFCKTFKFEIVKTYNSIKILEDEGYIVLSEAVETKSRVKFKLQRDDLYKFQ
                     VEHRQFDAFIKLLLRSYTGIFTEFCIISEEQLAKKVNAETDLIIKYLIALAKLGIISY
                     YRAKQNPLIIYTEERLPTESLLFSYNDIETRKQKHEQRLQAIIEYVTNHSKCRSLQLL
                     EYFGESNKMRCGNCDVCRKRNEIGVNTITFDYIVSDLKRIIGKKPASLHEIISQVSHS
                     EQDVISVINYLLDHKKISYTGGSELLWNT"
BASE COUNT         1653 a          807 c          806 g         1799 t
ORIGIN      
        1 aatggtaaat gtgtataaag atgattatac aattctatca atacaatcaa cggtattaaa
       61 tactcctaaa aatccggtat gtgtaggaga tcaggtacgt ttaactgctc aaaccagttc
      121 tcctactcaa cgctacttat ggtctacatc gccttatttt tttcctattt taaattcaga
      181 ttttaaacaa aatagtattg tggttaatcc tacagaaact actatgtatt atgttcatac
      241 attaagtatg ttttgcgaat atgaagatat tgattcaata ttggtagaag ttgccgataa
      301 caaaattata gcaaccggcg atactcttat atgtaagaat gatgttgcta ctatttcagt
      361 tcaaaatctt ataaatggtt catccctttc gtataattgg actcctaaaa gtgcagttgt
      421 gtccggtggt gattcggaaa atgctatagt aagaccggag cgatcaacac aatttatagt
      481 atatgctacc aattatgccg gatgtatagt tcaagatact gtacaagtga atgtagatga
      541 agttaaaata gaaacaagtg tttataatcc aataagttgc catggacaaa ccgatgcgtc
      601 tatagtctta tcacccaaag gtattgaacc atatacctat gaatgggaaa atggattaaa
      661 ttcttcgcaa agatctcaat taggagaagg aaaatatata gttactgtaa ccgatggatt
      721 aggttgtata aatactcgtg agtttgtaat tgaagaaccc gatttgttgc aaataattga
      781 tacatcaatt tggtatgtaa catgtgatgc tgcttgtaat ggggctattg atataactat
      841 ttctggtgga acattaccat atgcgtattt gtggtctcgt ggtgatacaa ccaaaactat
      901 tcagtcattg tgtcaaggtc tttataatgt aaatgttaca gatgctcatg gttgtaaaac
      961 aagtttagca caagctgtta taattggcaa acatgaacgg ctaccgtatt tagatgcatt
     1021 tgccgaacaa tcaattttgt ttaaaggtca atctactcaa ttacatgctt ctcctattcc
     1081 tattgatacg gtatcgtatt tttggtatcc aaatatttgg atggaaaatt acgcattgcc
     1141 aatagtgact gttgtaccac aagattcttt tacctattat gtaagagcta tagatacata
     1201 tggttgtgaa tctgttgata ctgttcaggt aactgtgcat gaatgggttt gtgatgatcc
     1261 gtttatttat attcctactg catttacacc caacaatgat gggaaaaatg atatgttata
     1321 tattaagagt tatgtaatta ctgatttgta ttttgcaatt tacgacaggt ggggtgaaaa
     1381 gctctttgaa acaactgata ttacaaaagg gtgggatgga acatatcaag gaaaagtatt
     1441 gcaacctcaa gtattagtat attatttaga agctacttgt ttgaatcaac aaaaatttaa
     1501 gtcaaaagga aatattacaa taattaaata accattgaaa cgaataataa ttagtatact
     1561 atatgcgtgt tttgtctttc aagcatttgg acaggatata catttttcaa atttttatac
     1621 taatgtatta aatctaaatc ctgcttttac cggttatttt aaaggaaaat atcggtataa
     1681 tcttacgtat agagacagat atagaactgt agctgtgccg tatcaaactg tatctttggg
     1741 aattgataca aaaacaagca aattacatgg attaagaaaa acgtttggat atggaatact
     1801 tcttaattat gatatagccg gagattcaca atttaatact attcaattac aatttccggt
     1861 ttcatatatt catattacta gtaataaaca atggaatctt tctgccggat tgatgcctgc
     1921 gcttgtgttt aattcattag actatacatt attgcgtttc cccgatcaat ttgatggagt
     1981 acaatataac cctaatttgc caatgaatga ggattttgat aaaacaaatt cgtttttttt
     2041 taatattgga gcaggaggac aggcaacata taaacctaat aaaacacaat catatactat
     2101 gggtttagca gggtacaatc ttacaacacc aaatatttct ttttttgatg ataaatcggt
     2161 tcaattatat gctagaatgt tgattcatgc aatggcaata gtagaagtaa agcccgatgt
     2221 tgacatggta ccggcagtaa aaatgcaatt tcaaggaaaa caaaaagaat atcatgtagg
     2281 agctatggca attaaatatt tcgataatct ttcaattatt gatgtatccg gcggagtatg
     2341 gtttcgttcg cgcgatagag atgcaataat agtaggtgta ggatgtaatt atttagggta
     2401 tacaatatta tgcaattatg atataaatat atcatcactt aaaaaagcaa gtaatggaca
     2461 tggagctttt gaattgacgg tttcatcaat actctatgaa caaaaaaaac gaaaaaaaat
     2521 gacttcggtt aaatgtccgc cgtatttata aattattttt ctaattcatt ccaagccaaa
     2581 gcactagcgc ctaaaacagc tgcgtttaca ttgagtaaac cactttgagc tatagtaatt
     2641 ttatttttaa aaataggtaa aagattttct tccatagctc gttttgtagg ttcaattatt
     2701 aaattacctg ctttagcaac tccaccaaat aagaaaatat gtgaaggact ggtatgacaa
     2761 acagcatcgg ctaatttaag acctaaaatg tgtcctgtat attcaaaaca ttctttagct
     2821 aattcatcgc catctttgag ggctaattct atgtctaatg ctgtaatttt attgtaggga
     2881 atatcacgaa gttttgatgg tttattagaa ttacataaaa tttcagccat ggttcttacc
     2941 atacctgttg cagacacata ggtttctaaa caaccttttt taccgcagcc acattgtctt
     3001 ccgccgggaa atacagtagt gtgaccaact tctcctgcaa atccgtcatg tccgtatact
     3061 aaatcaccat taactactat accacttcct aatccggtac ctaaagtaat cactataaaa
     3121 tcacgaattc ctctggcagc accgtatatc atttctccca tggctgcagc gtttgcatcg
     3181 tttgttatag ttacaagtat atttggaaaa taagaaccta acattttaca aaaatatacc
     3241 gttcctttcc aactcaaatt tggtgcttct tctattgtgc ctctatgata gttagcgttg
     3301 ggtgctccaa ctcctattcc tacaagttca aactcaggtt tttgtgttaa ttcttgttga
     3361 ataataccgg ctaaattctt tatatatggc tctatttcaa catgttcttt ggtcggaata
     3421 gatgtttcaa atacacaatt tccttctctg tcaacaaatc caatagcggt atttgttcca
     3481 cctatatcaa ctcctacggt aatttgtttg ttcatacgtt tttacttttt cacaaaaata
     3541 taataattgc aacaattttt aattgaatat atacaataaa agtatagtaa tgtacatggt
     3601 aaaacataaa ttaaaagtat atgttatgta ttccatagta attcagaacc tccggtgtag
     3661 ctaattttct tatggtcaag tagataatta attacagaaa ttacatcttg ttcggaatgt
     3721 gaaacttgag atataatttc atgcaatgaa gccggttttt taccaattat tcgttttaaa
     3781 tcactaacaa tatagtcaaa tgtaattgta tttacgccta tttcatttcg ctttctacat
     3841 acatcacaat taccgcagcg cattttgtta ctttcgccaa aatattccaa taattgtaaa
     3901 cttctacatt tagaatgatt tgtaacatat tctataattg cttgtaatcg ctgttcatgt
     3961 ttttgttttc gtgtttcaat atcattatat gaaaataaca atgattcagt aggcaatcgt
     4021 tcttctgtat aaataatcaa tggattttgt ttggctcggt agtaacttat gatacccaat
     4081 tttgccaaag ctataagata ttttataata aggtctgttt cagcgtttac tttttttgcc
     4141 aattgttctt cagagattat acaaaattct gtaaaaatac ctgtatatga acgcaataaa
     4201 agtttaataa atgcatcaaa ttgtctatgt tcaacttgaa atttatataa atcatcgcgt
     4261 tgtaatttga attttacacg tgattttgtt tctacagctt ctgaaagtac aatatatcct
     4321 tcgtcttcca gtatttttat actattatat gtttttacaa tttcaaattt aaacgtttta
     4381 caaaagtcat caataaagaa atcataaacg tttcctttgg cagcaccaac ttctatttga
     4441 taataattag ctaaagcttc gtatacattt tttatgtatt taaaatcagg gaattttttt
     4501 gtaaaatttg cttttaagtt agaaatatct tttttattat acaatagcac ggcaaaagct
     4561 tcaagaccat ctcgaccagc tctgcctgct tcttgaaaat atgcttcaag tgattcgggc
     4621 atgtcaaaat gtaaaacaaa tcgaacattt ggtttgtcaa tacccattcc aaatgcattg
     4681 gttgaaatca ttaccatatt atgagatttc atccaatttt cttgttttaa atttcgtgtt
     4741 tgtgcatcga gtccggcatg ataaaaatct gtatttacac ccatctctct cagagctagg
     4801 gctatttctt tagtttgttt gcgtgtttta acatatacta ttccggcacc tcgtattttt
     4861 ttgcaaatag ttaaaatata acctaattta tcttcttttt gtttaactat atatgttaga
     4921 tttttccgtt caaagcttgt ttgcaataca ttatggcgtt taaaccctaa tttgttttgt
     4981 atatcggcta ctacatcggg agttgctgtg gctgttagag ccaaaacagg aacagtaggt
     5041 attacatttc ttatttcaat tattt
//
LOCUS       DAOTOW010000061         5006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13890_length_5006_cov_2.940416, whole genome shotgun sequence.
ACCESSION   DAOTOW010000061 DAOTOW010000000
VERSION     DAOTOW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13890_length_5006_cov_2.940416"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..381)
                     /locus_tag="P5243_01695"
     CDS             complement(97..381)
                     /locus_tag="P5243_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRS18189.1"
                     /translation="MTKADIVNEVSKKTGIEKVIVQKTLESSMDAIKDALKQGSNVYL
                     RGFGSFIVKKRAEKTARNISKNTTIIIPQHNVPAFKPAKTFVSVVKNNVK"
     gene            complement(489..1217)
                     /locus_tag="P5243_01700"
     CDS             complement(489..1217)
                     /locus_tag="P5243_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18190.1"
                     /translation="MKKHLPVILGILGILSFIVISCKEPNFCMDCDSEGIVKTTVSYG
                     DSASITIYPVITPLNYNHCDSFVLYNGTYVEPYKSPKYKDSLKLCTAKEAQLEANEGK
                     HLCVCRENPNVKFNDDIKDINNNQGINNFFYIQGINKFPYNKLFIKVQDDTTKIRTYK
                     NYDNINDKFTGLVLDTMDIEYVNSKMLRSGVYIYELVLYKDQNHQVALDTISGKFFIV
                     RSNEDCNKNCLKHAYNQNDTKLLK"
     gene            1306..1947
                     /locus_tag="P5243_01705"
     CDS             1306..1947
                     /locus_tag="P5243_01705"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS18191.1"
                     /translation="MFHKYPHLTFPLLFVLLILCIHYCAVVYSFSLIQFGIIPRNFHG
                     LTGILFAPLIHSSWEHVLSNVSSLIITLYILFTVFKTVSHIVLVTGYIVPGIITWFIG
                     RSAIHIGASGVVYCLLFFMVCIGVYTKKRQLLAVSAVIIVLNSGFIWGILPHNNSISW
                     EYHIGGAVTGCFLAFLFRSFSFTKPEVDSLQNTNVSSTAQGIEFIYTKKPDSH"
     gene            complement(1986..4109)
                     /locus_tag="P5243_01710"
     CDS             complement(1986..4109)
                     /locus_tag="P5243_01710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS18192.1"
                     /translation="MSAIQTLRNKSGLITLIIGIALLSFVITGLDPKLFSSFKTPENV
                     IAKIYGEDYPYEAYYEYYEQAEKQFRNSEFQNAFQADALYNQAWQTFLNSALFDIQFK
                     ELGLGVYRDNLQIMGLSDKEFEDITIGENIDSELQRYFQNPKTGMFDKQMLIQTLANL
                     SEYKESNPEFYESWIDYEKALHINNLRNKYNTLVRNALYTTSLEAEMSINDRKTQTDI
                     EYVKIPFESIADETVKVSDSEIAAYYEKVKKSKKYDQEKAVSIDYVTFDITPTPQDIE
                     EIKKAVLSKAEDFKTAKNTSVFLNLNSDSKFDPTYHKKGTLPPAIDSFAFTKAKNDIT
                     EMYLEGNVYKIAKISDIRMASDSAKARHILVTGQNAMKEADSLKALLEKGADFGTLAM
                     KHSKDSLSAIKGGLIDWFKEGQMVPSFQDTCFFGEKGKIYLAPSNYGIHIIEILEQGK
                     KSKRVQVQYLSKQITYSQNTRKEVYKKAVEFASENRTKEQFEAYLAKNTTISKKIGEK
                     LGENERSLPGIQDSRAVIRWAHTNKELDKVSDIFTCGDKFVIAVVSKVYEKGKIPLDD
                     IKEQIKAEVIKEKKIAMIKEKLAGINTQTTSLSEIGLKFNIVALTSLNISFAMYSSPE
                     FGMEPKVIATACILEKGKQSQIIEGNTGIFVIKVVDRRTIDTNSDIQVEKTMLTQRAS
                     SQLMNATPAILKDKAEVEDLRINFM"
     gene            complement(4187..>5006)
                     /locus_tag="P5243_01715"
     CDS             complement(4187..>5006)
                     /locus_tag="P5243_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRS18193.1"
                     /translation="GIIAGNIFYILILLIFYIEYTHIFKFVVTDTGMQGTGNTPIPVN
                     TNDEETIKLFKNAVEFSSVKLRECSIPRTELVTIQTTDSLELLTQKFMSSGYSKVLVY
                     EDSIDNIIGYVHISDMFKHPQSITEIIRPIQFVPETMNAKKLMKQLIQNKQSIAVVVD
                     EFGGTSGVVTIEDIIEEIFGEIKDEFDTITLEERKLPNNEFIFSARHEIDYINEKYEL
                     NLPSSDDYDTLAGLFLYHHQKFPHINASIIIEQCKLTILKISGTHLELIHIKKI"
BASE COUNT         1472 a          718 c          731 g         2085 t
ORIGIN      
        1 ctgtttttac gtagtctttt tttacgttta tgtgtagcca ttttatggcg tttacgtttt
       61 tttccgcttg gcatagttat tttcctccga aattaattat ttaacattgt tttttacaac
      121 agatacaaat gtttttgcag gtttaaatgc aggaacattg tgttgtggta ttataatagt
      181 tgtgttttta gaaatattac gagctgtttt ttctgcacgt ttttttacaa tgaaacttcc
      241 aaaaccacgt aaatatacat tactaccttg tttaagagca tctttaatag catccattga
      301 tgactctaat gttttttgta ctattacttt ttcaatgcct gttttttttg aaacttcgtt
      361 tacaatatca gcctttgtca ttttcttata tttttaatta tcaaacattt aactttaatt
      421 ccctaattgg gagggcaaat atacggttta taattgtaaa taaaagaatt ttataagaaa
      481 aaattctttt attttaacaa tttagtatca ttttgattgt aagcatgttt taaacaattt
      541 ttgttacaat cttcgtttga gcgtactata aaaaatttac ccgaaatagt atctaatgct
      601 acttgatgat tttgatcttt atataaaact aattcatata tatatactcc ggaacgtagc
      661 atttttgagt ttacatattc tatatccatt gtatctaata cgagtccggt aaatttatcg
      721 tttatattgt cataattttt atatgttctt attttagtag tatcgtcttg tacttttata
      781 aataatttat tgtatggaaa tttgttaatt ccttgtatgt aaaaaaagtt gttgatccct
      841 tgattattat ttatgtcttt aatatcatca ttgaatttta cattcggatt ttctctacat
      901 acacataaat gtttgccttc gtttgcttct aattgagctt ctttagctgt gcataatttt
      961 aaactatctt tgtatttagg tgatttgtaa ggttctacat aggtgccatt gtataaaaca
     1021 aatgaatcgc aatggttgta attgagggga gtaattaccg gataaatagt aattgatgca
     1081 ctatctccat atgatacagt tgttttaact attccttctg aatcacaatc catacaaaaa
     1141 ttaggttctt tacaacttat aactataaaa gaaagaatac ctaaaatacc taaaataact
     1201 ggcaagtgtt ttttcatatc gctcattttt ttatttaaac atgctaatat acaattatat
     1261 ttatacaaat gtactatttt cgttgataat ttcaaaaaaa tagtaatgtt tcataaatat
     1321 cctcatttaa cttttccgct tttatttgtt ttactgatac tctgtattca ttattgtgct
     1381 gttgtttatt cattttcgtt aattcaattt ggtataattc ctcgaaattt ccatgggctt
     1441 acaggtatat tatttgctcc tcttattcat agttcatggg agcatgtttt gtctaatgtt
     1501 tcatcgctta ttataacttt gtatatatta tttactgttt ttaaaacggt ttctcatata
     1561 gttttggtta ccggatatat agttcccggt attattactt ggtttattgg aagaagtgca
     1621 atacacatag gtgcaagtgg ggtagtatat tgtttattgt tttttatggt gtgtataggt
     1681 gtatatacca aaaaacgtca attattggca gtttctgctg taattatagt gctcaatagt
     1741 ggttttattt ggggaatatt gcctcacaac aattcaattt catgggaata tcatataggc
     1801 ggtgctgtta ccggttgctt tttagcattt ttgtttcgaa gtttttcttt tactaaacct
     1861 gaagttgatt ctttgcagaa tacaaatgtt agttctacag ctcaaggcat tgaatttata
     1921 tatacaaaaa aacctgactc gcattgaatc aggtttttat tgtttagtat ttatcctctc
     1981 aaaaactaca taaaatttat tcgtaaatct tctacttcag ctttatcttt caatattgcc
     2041 ggagttgcat tcataagttg tgacgaagct ctttgagtta gcattgtttt ttcaacttgt
     2101 atatctgaat ttgtatctat agttcttctg tcaacaactt ttataacaaa aataccagta
     2161 ttgccttcta ttatttgcga ttgttttcct ttttcaagaa tacacgcagt ggcaattact
     2221 ttaggttcca taccaaattc cggtgatgaa tacatagcaa aacttatatt gagtgatgtt
     2281 aatgctacta tattgaattt taatccaatt tctgataatg atgttgtttg tgtattgata
     2341 cctgctaatt tttctttaat cattgcaatt ttcttttctt taataacttc ggcttttatt
     2401 tgctctttta tatcatctag aggtatttta cctttttcgt acacttttga aacaacagca
     2461 attacaaatt tatcaccaca ggtaaaaata tcagaaactt tatctaattc tttatttgta
     2521 tgagcccagc gaattactgc acgtgaatct tgaataccgg gtaatgaacg ttcgttttcg
     2581 cctaattttt caccaatttt ttttgatata gttgtatttt ttgctaaata agcttcaaac
     2641 tgttcttttg ttctattttc tgaagcaaat tcaacagctt ttttgtatac ttctttgcgt
     2701 gtgttttgtg aatatgtaat ttgttttgat aggtattgaa cttgaactcg ttttgatttt
     2761 ttgccttgtt ccaaaatttc tatgatatga ataccatagt tagaaggagc taaatatatt
     2821 tttccttttt caccaaagaa acatgtgtct tgaaatgatg gaaccatttg tccttctttg
     2881 aaccaatcta ttaagcctcc tttgatagca gataaactgt ctttagaatg tttcatagca
     2941 agtgttccaa aatcagctcc tttttcaagt aaggctttta aactatcggc ttctttcatc
     3001 gcattttgtc ctgtaactaa tatatgtcgt gcttttgcag agtctgaagc catacgaata
     3061 tcagaaattt tagctatttt atatacattt ccttctaaat acatttctgt aatgtcattt
     3121 tttgctttag taaacgcaaa tgaatcaata gccggaggta gtgtaccttt tttatgatat
     3181 gtaggatcaa attttgaatc agaatttaaa tttaaaaata cagatgtatt ttttgctgtt
     3241 ttaaaatctt cggctttgct caaaacagct tttttaattt cttcaatatc ttgtggggtt
     3301 ggggttatat caaatgttac ataatcaata gaaacagctt tttcttgatc gtattttttt
     3361 gattttttta ctttttcata gtatgccgca atttccgaat cgcttacttt aactgtttcg
     3421 tctgcaattg attcaaaagg aattttaaca tattctatat cagtttgcgt ttttctgtca
     3481 tttattgaca tttctgcttc aagagatgta gtgtataatg cgtttcttac tagtgtatta
     3541 tatttgtttc gaagattatt tatgtgtaat gctttttcgt aatcaatcca actttcataa
     3601 aattcaggat ttgattcttt atattcactt aggtttgcaa gagtttgaat aagcatttgt
     3661 ttgtcaaaca ttccggtttt gggattttga aaatatcgtt gtaattcaga atctatattt
     3721 tctccaatag taatatcttc aaattctttg tcagagagtc ccataatttg taaattgtcg
     3781 cgatatactc ctaatcctaa ttctttgaat tgaatgtcaa aaagagctga atttaaaaat
     3841 gtttgccaag cttggttata taacgcatca gcttgaaatg cattttgaaa ttccgaattt
     3901 ctgaattgtt tttcagcttg ttcgtaatat tcatagtatg cttcgtaagg gtaatcttct
     3961 ccgtaaattt ttgcaataac gttttcgggt gttttaaatg acgaaaataa ttttggatcg
     4021 agtcctgtta taacaaatga taataaagca atacctataa ttaaagtaat gagcccagat
     4081 ttgtttctaa gagtttgaat tgctgacatt gttttcttaa aatttaatta acgatttatt
     4141 ttcaagttgg caaagataaa aaaaatacga tatgaaacat ggttccttat atttttttta
     4201 tatgaattaa ttctaaatgt gttcccgaaa ttttgagaat agtaagttta cattgttcta
     4261 taataataga agcattgata tgaggaaatt tttgatggtg atataggaaa agacctgcaa
     4321 gtgtatcata gtcatcagat gatggtaagt ttagttcata tttttcgttt atatagtcaa
     4381 tttcatgtcg agccgaaaaa ataaattcat tatttggtaa ttttcgttct tcaagagtta
     4441 ttgtgtcaaa ttcatctttt atttctccaa aaatttcttc aataatatct tcaattgtta
     4501 caacacctga tgtgcctcca aattcatcta caactacagc aatactttgt ttattttgta
     4561 taagttgttt catgagtttt tttgcattca tggtttcggg aacaaattgt atggggcgta
     4621 tgatttcggt tattgattgg gggtgtttaa acatgtctga tatatgtaca tatccgatga
     4681 tattgtcaat agaatcttca tataccaata ctttagaata cccgctcgac ataaattttt
     4741 gtgttaataa ttcaagcgaa tctgttgttt gtattgttac taattctgta cgaggtatgc
     4801 tgcattctct taattttact gatgaaaatt caacagcatt tttaaataac tttatagttt
     4861 cttcatcgtt tgtatttacc ggaataggtg tgtttccggt tccttgcatt cccgtatctg
     4921 taaccacaaa tttaaatata tgagtatact caatataaaa tataagtaaa attagaatat
     4981 aaaaaatatt tcctgcaatt attccc
//
LOCUS       DAOTOW010000062         5004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13901_length_5004_cov_3.201051, whole genome shotgun sequence.
ACCESSION   DAOTOW010000062 DAOTOW010000000
VERSION     DAOTOW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13901_length_5004_cov_3.201051"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..473)
                     /locus_tag="P5243_01720"
     CDS             complement(105..473)
                     /locus_tag="P5243_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18194.1"
                     /translation="MKKIIILLLIAVSSLVFADHGWERVNYMQSTIFSAEVTVNNKPA
                     LADDVVGAFVNNECRMIAKVFMVGDKAYVSSVIHGDSPEQVEFRLWLHQKDSIVVSPQ
                     KVATTPGGSLLHFSLNFVMK"
     gene            670..1317
                     /locus_tag="P5243_01725"
     CDS             670..1317
                     /locus_tag="P5243_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRS18195.1"
                     /translation="MLQYNFVNIKSIDSTIICDLRYSTTNNFVGIDMYGDFNACYVPL
                     DIAIRLHEVQKTLQLIDSAYSLVILDAVRPLHIQQIMWDSCTYSGRQKKNFLAHPSVT
                     SLHNYGAAVDVTLAYNGIEVDMGTPYDFAGEAAYTYIENELLAYNRISKEQYYNRQLL
                     RSAMKKLGFIDNNYEWWHFGACYRSQVSKKYPFIVSFDSITPVSKNSKILTEGSY"
     gene            complement(1370..3259)
                     /locus_tag="P5243_01730"
     CDS             complement(1370..3259)
                     /locus_tag="P5243_01730"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HRS18196.1"
                     /translation="MEPLVNVLKKQNIELPHQATVLLNSCKSFTVFNSVEELADAAVG
                     GKDSNSFEVTYAVPGKGDVTEVVVHRVKNGISANYTDPYMRRRDPDTMAVADSNVTDK
                     ESFHSKYGYEFSKLQDETFAWLQSQDLALFFYFAGRDGIGMGGLAIAPANAGFFAMGL
                     SMLQNILPISELPKDFAIQSIIYVAPTFRHTHFNGKQVVVHNRTEHVHEIYSYNLYPG
                     PSAKKGLYGALLQKGENEGWVTTHCSTVQVVSPYDNITTFMHEGASGGGKSEMLQNIV
                     RETNGQVLVGENTITGERRLINFPIFCSFNPVTDDMALCHPILQKNNGKLTVLDAENA
                     WFIRVDSVLDYGDDPFLEHLTIKPKGPLLFLNIQSVPNSTALIWEHTEDAPGKRCPNP
                     RVIVPRSIVPNVVNNPVTVDIRSFGVRTPICTRENPTYGILGMFHLLPPALAWLWRLV
                     SPRGHANPSIVGGGGMESEGVGSYWPFATGSMIAHANLLLEQIIKNPRMKYTLVPNQH
                     IGVWKVGFKPQMLMREYLTRRGNAKLRSDQYQNARCSLLGYELNYLTLEGAKIPSRFL
                     QVHKQPEVGVEGYDAGAAILMDFFRQELQKYDSPDLLPLGKKIIEACLNGASVQDYEA
                     FIPSM"
     assembly_gap    3671..3680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3681..>5004)
                     /locus_tag="P5243_01735"
     CDS             complement(<3681..>5004)
                     /locus_tag="P5243_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS18197.1"
                     /translation="NNQDFIVHIVEQGQTVYSIARMYDIAPVKILEANPDYTENLPIG
                     AKLIIPKIKQTQPVKQESIAEQSKINDKKDTPQVSEKSKETSVVPDKKIDNNISAKQE
                     QVREVVKEVSQKKQSVRIVLMLPFMLDINSKEYTEVEIINKQIQIHASTFPFIEYYEG
                     VLLAVDSLRKKGISVQLEVFDSSKDSIYIQEILSKINTHNLDLIIGPVFPVAFSIASE
                     FAKKYKIPIVSPLSSEDIALKTNPYVIQVNSPQRYRFKEIAKFASEYKQCNVIFVYNS
                     IELEQAQVQECKRIFSVYYPDSIKKNSITFKEVYFPKMGLDGVEKSLNNSSKNVIIVL
                     SRNQAFINNLVTKLYQYSKKFDVHLVGLPMWEKYDNLELDFLFALQFQFVTNGYVDYT
                     KDEVKKFIELYRTLYKTEPTKFSFQGYDQMMFFSELLSAWEETYKKGQL"
BASE COUNT         1637 a          872 c          687 g         1798 t
ORIGIN      
        1 ctaaatttta attaataatt atttttgttt gtttgatttt gctttttttt tgtgtccaca
       61 aatgtatata tttttttttt acatcaacta tgacgaacgt ttttttattt cattacaaag
      121 tttaatgaaa aatgtaacaa acttcctccg ggtgtagttg caactttttg aggtgacact
      181 acaatagaat ctttctgatg caaccatagt ctaaattcaa cttgttcggg agaatctcca
      241 tgtataacag aagacacata tgctttatct cctaccataa atacttttgc tatcattcta
      301 cactcattat ttacaaatgc accaactaca tcgtcggcta aagcaggttt attgttaact
      361 gtaacttcag cagaaaaaat tgtagattgc atataattta ctcgttccca cccatgatcg
      421 gcaaacacaa gtgaactaac tgcaatcaat aggagtatta taattttttt catactttta
      481 ccacgattta tttattgact aaattatact tttttcttga aaactatcgt aaaaattgta
      541 tttttttctt tttttattgt tgcatgtaac aaaaaagatt ccaaaattct atataaaaaa
      601 tatatagata ttgaaacggt ataccttcca attgaaacta aaagttctct tctacctttt
      661 gaaaaactat tgctgcaata taattttgta aatataaaat ctattgattc tactataatt
      721 tgtgatttac gatatagtac cacaaacaat tttgtaggaa tagatatgta tggcgatttt
      781 aatgcatgct atgtaccttt agatatagct attaggttgc atgaagtaca aaaaacatta
      841 caattaattg attctgcata ttctttagta atattagatg ctgtaagacc tttgcatata
      901 caacaaatca tgtgggattc atgtacgtat agcggtcgac aaaaaaaaaa ttttttagca
      961 catccttctg ttacatcgtt acacaattat ggagcagcag tagatgtaac gttagcatat
     1021 aatggtattg aagtagacat gggtaccccg tatgattttg caggagaagc ggcatatacc
     1081 tatattgaaa atgaattact tgcctataat agaatatcaa aagaacaata ttataacaga
     1141 caattattac gttctgccat gaaaaagcta ggatttattg acaacaatta tgaatggtgg
     1201 cattttggag cttgctaccg ttctcaagtt tctaaaaaat atccattcat tgtttcattt
     1261 gattcaatta ctcctgttag caaaaattca aaaattctta cagaaggttc ttattaaaaa
     1321 aaacaattat acggttctca ataaaccgta taattatttt aaactgtttt tacatacttg
     1381 gaataaatgc ttcataatct tgaactgaag caccattcaa acaagcttca ataatttttt
     1441 tacctaaagg taacaaatca ggcgagtcat atttttgcaa ttcttgacga aagaaatcca
     1501 ttaaaatagc agctcctgca tcatagcctt ctacgccaac ttcaggttgt ttatgaactt
     1561 gtaaaaaacg tgaaggtatt tttgctcctt ctaatgttaa ataatttaat tcatatccta
     1621 acaatgaaca tcgagcattt tgatattgat cgcttcgtaa ttttgcattt cctcttcgtg
     1681 ttaaatattc acgcataagc atttgaggtt taaatcccac tttccaaaca cctatgtgtt
     1741 gatttggcac caacgtatat ttcatgcgtg gatttttaat tatttgctcc aataataaat
     1801 ttgcgtgagc tatcatactt ccggtagcaa atggccaata tgaaccaaca ccttcacttt
     1861 ccattcctcc accgccaact atacttggat tggcatgccc acgaggcgaa actaaacgcc
     1921 acaaccacgc aagagccgga ggcaacagat gaaacatacc caaaattcca taggtaggat
     1981 tttcgcgtgt acaaattggt gtacgtacac caaaacttct tatatctact gttaccggat
     2041 tatttacaac attaggaact attgaccgag gaactattac tcgaggattt ggacatcgtt
     2101 ttcccggagc atcttcagtg tgttcccata ttaaagctgt agaatttggc actgactgta
     2161 tatttaaaaa taacagtggt cctttgggtt ttatggtcaa atgttctaaa aatggatcgt
     2221 caccataatc taaaacacta tcaactctta tgaaccaagc attttcagca tctaacaccg
     2281 ttaactttcc attgtttttt tgcaatattg gatgacacaa tgccatatcg tctgttacgg
     2341 gattgaatga acagaaaatt gggaaattta taagtcgccg ttctcctgtt attgtatttt
     2401 ctcctactaa tacttgtcca tttgtttctc ttacaatatt ttgcaacatt tcacttttac
     2461 caccgccgct tgcaccttca tgcataaacg ttgtaatatt atcatacggg ctcacaactt
     2521 gtactgttga acaatgcgtt gtaacccatc cttcattttc acctttttgc aacaatgctc
     2581 catataagcc ctttttagca cttggcccgg ggtataaatt atatgaataa atttcatgca
     2641 catgttctgt cctattatga actactactt gtttcccatt aaaatgagta tgtctaaatg
     2701 ttggtgctac atatatgata gattgaatag caaaatcttt tggtaattca gaaataggta
     2761 gtatgttttg aagcatagat aaacccattg caaaaaaacc agcatttgca ggagctatag
     2821 ctaaaccacc cattcctata ccatcacgac ccgcaaaata gaaaaataaa gctaaatctt
     2881 gagattgtaa ccaagcaaat gtttcatctt gtaattttga aaattcataa ccgtactttg
     2941 aatgaaaaga ttctttgtct gtaacattac tgtctgccac agccatagta tcgggatcgc
     3001 gacgacgcat atacggatcg gtataatttg ccgatattcc atttttaact ctatgcacta
     3061 ctacttctgt aacatctcct ttacccggaa ctgcatacgt tacttcaaaa ctattactgt
     3121 ccttgcctcc taccgctgca tctgctaatt cttcaacaga attaaataca gtaaaagatt
     3181 tgcaactatt caataatact gttgcttggt gaggtaattc tatgttttgt tttttcaaaa
     3241 catttactaa tggttccata cgttttcatt tttatgcttc tgtagtttaa tatacatcag
     3301 ctgattaaca atatgggcaa atatatattt caaaaaaccc cattctcatt ttttgatacc
     3361 aaatattcaa tataattttt taaaatactg aaaggtattt tttcatgagt agtatcagat
     3421 aaaatacaaa ggaatttata cagaactcaa cataacattc tgaatataaa gaaaataaaa
     3481 caatcaaaat actagtattg taaagaagta atatataatt acttatattt tttttcaaac
     3541 gttttggtat aataaaccgt ttgagcagat gtatttacta taccggaaga actttgttcc
     3601 cttacaaaca tataggtatc atgcaatccc gtttttgaaa cttgcgtaat tttctgcagc
     3661 aaggcatcat nnnnnnnnnn tcagttgacc ctttttgtat gtttcttccc atgctgataa
     3721 tagttcagaa aaaaacatca tttgatcata tccttgaaaa ctaaattttg taggttctgt
     3781 tttatacaaa gttctataga gttctataaa tttcttaact tcatcttttg tataatcaac
     3841 atatccattt gttacaaatt gaaattgcaa agcaaataaa aaatctaatt ccaaattatc
     3901 atatttttcc cacataggta atcctactaa atggacatca aatttttttg aatattgata
     3961 taattttgta actaaattat tgataaaagc ctgattacga gaaagcacaa taataacatt
     4021 tttacttgaa ttatttaatg atttttctac tccgtcaagc cccattttag ggaaatatac
     4081 ttctttaaat gtaattgaat tctttttaat agaatcggga taatatacac taaaaatacg
     4141 cttacattct tgaacttgag cctgttctaa ttcaatagaa ttatatacaa atattacatt
     4201 acactgttta tactccgaag caaattttgc aatttcttta aatctatatc gttgtggact
     4261 attcacttgt atcacatacg gattcgtttt gagagcaatg tcttcacttg ataatggtga
     4321 aacaattggg attttatatt ttttggcaaa ttcagacgct atagaaaaag caacaggaaa
     4381 aaccggacca attattaaat ctaaattatg tgtatttatt tttgataata tttcttgtat
     4441 atatattgag tctttactcg aatcaaatac ttctaattgc acagaaatac cctttttacg
     4501 caaagaatca actgctaaca atactccttc ataatactca ataaaaggaa acgttgaagc
     4561 atgaatttgt atttgtttat taataatttc tacttcagta tattctttag agtttatatc
     4621 taacataaat ggcaacataa gaactatacg aactgactgt tttttttgtg aaacttcttt
     4681 taccacttcg cgaacttgct cttgttttgc cgatatattg ttatctatct ttttatctgg
     4741 aacaacagat gtttctttac ttttttcact tacttgtggc gtatcttttt tatcatttat
     4801 cttactttgc tctgcaattg attcttgctt cacaggttgt gtttgtttta tttttggtat
     4861 tattaattta gctccaatcg gcaaattttc agtatagtca ggatttgctt caagaatttt
     4921 gaccggagct atatcataca ttcgagcaat agaataaact gtttgccctt gttcaacaat
     4981 atgaacaata aaatcctgat tatt
//
LOCUS       DAOTOW010000063         5001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13910_length_5001_cov_2.543874, whole genome shotgun sequence.
ACCESSION   DAOTOW010000063 DAOTOW010000000
VERSION     DAOTOW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13910_length_5001_cov_2.543874"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3728)
                     /locus_tag="P5243_01740"
     CDS             complement(<1..3728)
                     /locus_tag="P5243_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18198.1"
                     /translation="MLIIIVGMILPATLFTQDEGTLKQLYNSIEKLLSQREYEKSIPL
                     IEQLLAANPSNANYNFKMAYAIIRGHSDKNPIPYLEKAIQNINFKYKSKYTQDGAPVD
                     ALWYYALQRYYKYEYDIAKDYFERYISLIPSIHTNYEPCKAYIQWCKTGPEYVKNPVK
                     VIHKNFYEATELPKFFHSALFSPDESIFIFTAEQNQNIDNYKVEHRSLNDDIFITYLK
                     NGSWTTPKPLSNSINSLRREACIGMHPNGKQLLLYREDPDGGNIYYCDLLDSAIWSAP
                     KKFPAPINSSANESHATISADGKTIYFTSDREGGYGGLDIYMCKLQANGEWGKAINLG
                     SSVNTPYFEESPHLQANSNLLYFSSNRPDGLGEFDVYRCELKNDSIAYNVQNIGYPIN
                     TPRNDMFFKTSIDGGKAYYSSPCKSTNGEYDFMMVEFLDQKLYPNIIIKGVVLNQNGD
                     TLKNIPITLFNLTVKDITDSTKVSKKTGFYTFNLHSKNKYFASIEYDEMVYFSKPFTF
                     NKYFSDYSFSNVIEVDPIIINDSLMHQDIGSFKVFKQNLQTTEVDNEGNAKNMFTVLE
                     KMPLQTISRTNNTISSKPNFVYTPPTIKEEVTKPDITLTVTEPEKKEPVTKITEIPDV
                     IEKPKEKPITKSSVKKQSLSADSLINIGISIFNQGDYTKSIENFNKAIAIIDTTKNIQ
                     TKIVGLEYIADAQFNSGQILQSLETHKQSLSTIESTQNTTAIKNKQQEIAEIYSNLWY
                     KDEAIKYLEKNLKIDNQNNNTEGLSQTYQDIAQVYLQHNNQEKAIEYLQKGVDLSKDE
                     KKKAEAYNALGVSYHQIQQILKAIEYYNKAIQTAAKVNDKKGLSQYQNNLGNAYFDLQ
                     KFEESMIHYKISLGLQQELLDERGAAIALYNIGNIYRLQQKYNSAIDNYTQSIELALK
                     QKSNDILAKNYFAMMLIYKIIKNYPLALEYYKKYFGIASPYQKDFSVLYNEQEKHSVT
                     EGEISLLQAQMKRTEEILKFEKQKYEQELELLKQKEHVLKITRIALLILAIGLFLMLI
                     LLLLRYRTRRKYFKQLSQQNTEILQKQEEIIVQRENLEQMNTLLEKLSIVASKTGNAV
                     AILKPDGSFEWTNTSFDSLYQTQATSMIEFAYNDQERLQIQQCITQLQSVQYETKRHI
                     GTTEAWVLCNLTPIIIDNELFKIIVLESDITAQKIAEQKIREQRDEIQIQAKEIEKQR
                     DIAIEQRNEIELQKNAVEKALQDLQMTQKKLIESEKMAS"
     gene            complement(3819..4442)
                     /locus_tag="P5243_01745"
     CDS             complement(3819..4442)
                     /locus_tag="P5243_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18199.1"
                     /translation="MKKITHILAWTISIIMHPIIYGLLGSFVLIFSLPYYASFNVSYS
                     IDYLTFVGLLTYIMPLVAIPVYYSIIKISKQNVSSNHQRIFLLLSTTIIYAMSYRILH
                     KIQVYQLVSYYILLCTVLLFISLVITYFWKISLHMIGIGGFTGILLILSYAQHPVPLS
                     ILPYSFLAAGSIAAARLYLNAHSSLQMYVGYITGLITSIFFTIIFII"
     gene            complement(4449..>5001)
                     /locus_tag="P5243_01750"
     CDS             complement(4449..>5001)
                     /locus_tag="P5243_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18200.1"
                     /translation="RNSDREVILVTAGAIASGIERLHLSGYPPTLAEKQAVAAIGQVE
                     LIKRYQNIFDEYTQMIAQVLLARDIINNPKQQKNAKNTFRKLLSLGIIPIINENDTIS
                     TADIEQENNYILTATVASITNAHALIVINKDFSFKVLCKGNNFYYAIASKEDLLHFLS
                     KLDIASLHKKKFSYPTIFPKQGM"
BASE COUNT         1510 a          680 c          802 g         2009 t
ORIGIN      
        1 gaagccattt tttctgattc aatgagtttt ttctgtgtca tttgtaaatc ttgcaatgct
       61 ttttctactg catttttttg caattcaatt tcattacgtt gttcaatagc tatatctctt
      121 tgtttttcaa tttcttttgc ttgtatttga atttcatcac gttgttctct aattttttgt
      181 tctgcaattt tttgtgcagt gatatcagat tcaagaacta ttattttaaa taattcatta
      241 tcaataatga ttggagtaag attgcaaagg acccaagctt cggtagttcc aatatgtcgt
      301 tttgtttcat attgaacaga ttgtaattga gttatacatt gttgaatttg taatctttct
      361 tgatcattat aggcaaattc aatcatagat gtagcttgtg tttggtataa tgagtcaaat
      421 gaagtatttg tccactcaaa actaccatcg ggtttaagaa ttgcaactgc attaccggtt
      481 ttacttgcaa caatagaaag tttttcaagc aaagtgttca tttgctctaa attttcgcgt
      541 tgtacaatta tttcttcttg tttttgtaat atttctgtgt tttgttgtga tagttgttta
      601 aaatatttac gtcgagttct gtatctcaac aataagagta taagcattaa aaataaccct
      661 atcgctaaaa ttaataatgc tattcgggta attttgagaa catgttcttt ttgtttcaat
      721 aactctaatt cttgttcata tttttgtttt tcaaatttta aaatttcttc ggttcgtttc
      781 atttgtgctt gaagtagtga tatttcacct tctgttacac tgtgtttttc ttgttcgtta
      841 tacaatacag aaaagtcttt ttgatagggt gaagctattc caaaatattt tttgtaatat
      901 tcaagtgcta acggataatt ttttatgatt ttgtatataa gcatcatagc aaaatagttt
      961 tttgccaaaa tatcattgct tttttgtttt aatgctaatt ctatactttg tgtgtaatta
     1021 tcaattgccg aattatattt ttgttgtaat ctgtatatat taccaatatt atataaagct
     1081 attgcagcac ctcgttcatc aagtaattct tgttgtagtc caagtgaaat tttataatga
     1141 atcatagatt cttcaaattt ctgcaagtca aagtatgcat tacccaaatt attttgatat
     1201 tgtgataatc cttttttgtc atttactttt gcagcagttt gaatagcttt attgtagtat
     1261 tctatggctt ttaaaatttg ttgaatttga tgatatgata ctcctaacgc attatatgct
     1321 tcagctttct ttttttcatc tttacttaaa tcaacacctt tttgtaaata ttctatagct
     1381 ttttcttggt tgttgtgttg taaatatact tgagctatat cttgatatgt ttgtgataat
     1441 ccttcagtat tattattttg attatcaatt tttagatttt tttctaaata tttgatagct
     1501 tcatctttat accataagtt actataaatt tctgcaattt cttgttgttt gtttttaatt
     1561 gctgttgtat tttgagtgct ttctattgta cttaaacttt gtttatgtgt ttctagagat
     1621 tgaagaattt gtccggaatt aaattgagcg tcggcaatat attcaagtcc cacaattttt
     1681 gtttgtatgt ttttagttgt atcaataata gcaatagctt tattaaaatt ttctatactt
     1741 ttagtataat ctccttgatt aaatattgat atgccaatat ttataagaga atcagcagaa
     1801 agactttgtt tttttactga tgattttgtt ataggttttt cttttggttt ttctattaca
     1861 tcaggaattt ctgtaatttt tgttaccggt tcttttttct caggttcagt tacagttagt
     1921 gttatatctg gttttgtaac ttcttcttta atagtaggcg gggtgtatac aaaattgggt
     1981 tttgaactta tggtattatt tgttcgtgaa atagtttgta aaggcatttt ttctaaaacc
     2041 gtaaacatat ttttagcatt accttcgttg tcaacttcgg tagtttgtaa attttgtttg
     2101 aaaactttaa acgaaccaat gtcttgatgc attagtgaat cgtttatgat aataggatct
     2161 acttcaatta catttgaaaa actatagtca ctaaaatatt tgttaaatgt aaatggtttt
     2221 gaaaagtaga ccatttcatc gtattctata gatgcaaaat atttgttttt agaatgaagg
     2281 ttaaatgtgt aaaatcctgt ttttttactg actttagttg agtctgtaat atcttttact
     2341 gttaggttaa atagagtaat tggaatgttt tttaacgtgt cgccgttttg attgagaaca
     2401 acacctttaa ttataatgtt tggatataat ttttgatcta aaaattcaac catcataaaa
     2461 tcatattcac catttgtaga tttacatgga gaagaataat atgcttttcc tccgtctata
     2521 ctggttttaa aaaacatatc atttctggga gtatttattg ggtaaccaat attttgaaca
     2581 ttataagcta tactgtcatt ttttaattca caacggtata cgtcaaattc tcctaatcca
     2641 tcgggcctgt tactactaaa atacagtaga ttactgtttg cttgcaggtg tggactttct
     2701 tcaaaatagg gagtgttcac acttgaacct aaattaattg cttttcccca ttctccgtta
     2761 gcttgcaatt tgcacatata aatatctaaa cctccatatc caccttctcg gtcactggta
     2821 aagtatatgg tttttccatc ggctgatata gtagcatgag attcatttgc cgaagaattg
     2881 ataggtgccg ggaatttttt tggagcagac caaatagcac tgtcaagtaa atcgcaataa
     2941 taaatatttc caccgtcggg gtcttcgcga tataataata gttgtttgcc gtttggatgc
     3001 attcctatac aggcttcacg acgtaaacta tttatagaat tacttagtgg ctttggggta
     3061 gtccaactgc catttttaag ataggttata aaaatatcat catttaatga acgatgttca
     3121 actttataat tatcaatatt ttggttttgt tcagccgtga aaataaaaat tgattcgtcg
     3181 gggctaaata gggcactatg gaagaatttg ggcaattcag ttgcttcata aaaattttta
     3241 tgaatcactt ttaccggatt ttttacatat tcaggacctg ttttgcacca ttgtatatat
     3301 gctttgcatg gttcataatt tgtatgaatt gaaggaataa gtgaaatata tcgttcaaaa
     3361 taatcttttg ctatatcata ttcatattta taataccgtt gtagagcata ataccacagt
     3421 gcatctaccg gagccccgtc ttgagtatat tttgatttat atttaaagtt tatgttttgt
     3481 atggcttttt ctaaatatgg tattggattt ttatcgctat ggcctcgtat tattgcataa
     3541 gccattttaa aattataatt ggcatttgat ggatttgctg caagtaattg ttctatcaat
     3601 gggatagatt tttcgtattc tcgttgcgaa agtagttttt ctatagaatt atataattgt
     3661 tttaaagtgc cttcatcttg cgtaaataat gttgcaggaa gtatcattcc aacaattatt
     3721 attaacattg acagagtgga cttacgaaac attatattct aaatattaaa accttatgcg
     3781 aatatacaac atatttgcgt aaaatcatgt atttagagtt aaataatgaa tatgatagta
     3841 aaaaaaatgc tggtaatgag accggtaata tagcctacat acatttgtaa cgacgaatga
     3901 gcgttaagat acagtcttgc agcagctata gaaccggcag ctaaaaaact atatggcagt
     3961 atgcttaatg gtacaggatg ttgggcatac gaaagaatta aaagtatacc tgtaaaaccg
     4021 ccaataccta tcatatgtaa acttattttc caaaaatagg ttattactaa acttataaac
     4081 agtaaaacag tacataacaa aatgtagtat gatactagtt gatacacctg aattttatgt
     4141 aaaattctgt atgacatggc gtaaataatt gtagtgctta ataataaaaa aatacgttga
     4201 tggtttgagc ttacattttg ctttgaaatt ttgataatac tataatatac aggaatggca
     4261 actaatggca taatataggt taaaaggcct acaaatgtta ggtaatcaat agaatatgaa
     4321 acattaaacg aggcataata tggtaatgaa aatattaata cgaaactgcc taataatcca
     4381 tatataatgg ggtgcatgat tatggaaata gtccaggcaa gtatgtgtgt aatttttttc
     4441 attagacgtt acattccttg tttaggaaaa atagtagggt atgaaaattt ctttttatgt
     4501 aacgatgcta tatcaagttt agaaagaaaa tgtaataaat cttctttgct ggcaattgca
     4561 tagtaaaaat tattgccttt acagagaact ttaaatgaaa aatctttgtt tattactatt
     4621 aatgcgtgtg catttgttat agaagctact gttgctgtaa gtatataatt gttttcttgt
     4681 tcaatatctg ccgttgatat agtatcgttt tcatttataa ttggaattat acctaaagaa
     4741 agtaattttc taaatgtgtt ttttgcattt ttttgttgtt tcggattgtt tataatgtcc
     4801 cgtgctaaaa gaacctgtgc tatcatttgt gtatattcat caaatatatt ttgatagcgt
     4861 tttataagtt caacttgtcc tatggcagca acagcttgtt tttcggccaa agtaggaggg
     4921 tagcccgaaa gatgaagtcg ttctataccc gatgcaattg ctccggcagt tactaatatt
     4981 acttctctgt cgctattgcg t
//
LOCUS       DAOTOW010000064         4988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13951_length_4988_cov_3.040746, whole genome shotgun sequence.
ACCESSION   DAOTOW010000064 DAOTOW010000000
VERSION     DAOTOW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13951_length_4988_cov_3.040746"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="P5243_01755"
     CDS             <1..1617
                     /locus_tag="P5243_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRS18201.1"
                     /translation="GTITGVINSNRLPELYMHFAYSDGSFTYNSNPFKVDLQGTLTTK
                     NMANLAFFNLSNCTYTLSRGETKLNGSLSVTDFNNPYFEILGTLQTKLEDISEFIQPE
                     NYIVTGNASGTFSCKGRIADIDSLLPSFFTKTHTEFNCKVTDLEITPPSYSPYEFKQV
                     NGTITYKDSKMTFEQIQGKLKGANFSLDGIGNNMLNYILFSNQDATFQGNFSITDINI
                     DPFEKHYYDYLCNEPSTSTLQTRINVTAKQVEYDNFTFQDVSCILIYTGNTFNLLQTK
                     LKTMKGLFAGDVYVTYYDNNTTKVEANGDFKKVSAKELFKTFNNFDQTFLTESQISGT
                     ISTKFNMSTMLDKDYNPIYSSMKILSNVIIEDGNISNFDPFIEMGKKMKVEEFKSVSF
                     TRIENILKIENDTLYIPNMRIQTNAFDMNFAGKHAINNNQFNYYITVFMKKTLSNMFK
                     KKNEGEDFGEIEQNTDGNLKLPLRIYGNPDKYSIEYDLKTSKQNVKQTMERQKEDWKA
                     ILNKQSNEEIQEKPKKQEKPIDSGFKIEYD"
     gene            1636..3141
                     /locus_tag="P5243_01760"
     CDS             1636..3141
                     /locus_tag="P5243_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18202.1"
                     /translation="MKLYSAKYKIKVALFTIAILFVGAIIYLLNTTITNIKQEERKQV
                     LLWAEAVQKRYTLVDYTNKLFEKLQEEERRKVELWSDAQHHLLYEDDSQFLTFLLKII
                     SSNKNIPIILTDAKGNVVSSVNTDFAIPLNKPMPDSIKQIFTEYNPIPILYDNEPINY
                     LYYTDSKLFSELQNVMNNMIQSFIEEVVKNAASVPVIITNKDTTQIIAYGNIQKYRIE
                     TNEKIFQTIQEMASSNPPIPIILNNSVVHYIFYEDSTLITRLRYYPTILIGIGFIVLL
                     FAYFALRSSEKFEQNQLMVGMSKETAHQLGTPISSLMAWVELLKQQNIDNDIVTEINK
                     DVTRLQTIAERFSKIGSAPNLNPHVLYDTIQKSLDYIQIRTSQTISFHLHCQQKNIVV
                     PLNIALFEWVIENICKNAIDAMQGKGSLSITIISQSPWVHIDIEDSGKGIPRNKFKTI
                     FKPGYTTKQRGWGLGLSLAKRIIEEYHNGKIFVKFSELNKGTTIRISLPMA"
     gene            3148..3987
                     /locus_tag="P5243_01765"
     CDS             3148..3987
                     /locus_tag="P5243_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRS18203.1"
                     /translation="MKIRDAFNILEISDDASPEEIKKAYRAKAKLYHPDVNNSNSAQA
                     EFECIQNAYTLIQAYIQYRESKHSHSEIQNTKIKRKQNSKQTKAAKKQWFEARIQYDI
                     SIFKKSKQYILSYCFAFIGSIVALGICIDYFSAPQENYEPIQHIEIPQNFHDIKNYAW
                     KELYIIVQDRRISITPLLLNQIQNTSYLKIYNTPYLQQIKRIEATNSQPYTEIEFYNF
                     YNDAFLIIVILLCGGISMFVFNKKSLGYYSIIRFYNLYCLPLIILFVLLGEARILRLF
                     GIV"
     gene            complement(4001..>4988)
                     /locus_tag="P5243_01770"
     CDS             complement(4001..>4988)
                     /locus_tag="P5243_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS18204.1"
                     /translation="TAIQLHGAFYSSPIVTKQGNVVIGNHRKKIYFFNSVGDKIQEFS
                     TKGWVHATPSIVSDTIVSVASYDGNMYFFNEIGSLLKQIKLHGSIFTNALQLDSSRVI
                     FGSNKKGVIFYNFIENTYTQFTTKKMVHGSPQLLSNKNICVGGNDKRMYIFNDSANLV
                     SSYLTNGWIMHSKALQVKDSTIVFGSYDRYLHAINLQGKLEWKFKTNGKIHASPQKVD
                     DKIVFGSFDKHIYILKNDGQLLHSIATKKRIVASPAITQDNFIVVGSYDKNIYVLNIN
                     GEIICKYFVDGKVFSTPVVLPNGTIFCATTNGKIYYLKPKVSYTDIQYSSVK"
BASE COUNT         1931 a          795 c          643 g         1619 t
ORIGIN      
        1 ggaactataa ccggagtaat aaattccaat cgactacccg aattatatat gcattttgca
       61 tattccgatg gttcttttac ctataattct aacccattca aagttgattt gcaaggaact
      121 cttacaacca aaaacatggc aaatcttgca ttttttaacc tatctaattg cacatacact
      181 cttagccgtg gtgagacaaa actaaatggt tctctttctg ttactgattt taacaaccct
      241 tattttgaaa ttttaggaac gttacaaact aaattagaag atatatcaga atttattcaa
      301 cccgaaaact atatagtaac aggtaatgca tccggcacat ttagttgtaa aggcagaatt
      361 gccgatattg actctttatt gccttctttt tttaccaaaa cacacacaga atttaattgt
      421 aaagttactg atttagaaat tactccccca tcttattctc cttacgaatt caaacaagta
      481 aatggcacta taacatataa agattcaaaa atgacttttg aacaaattca aggaaaactt
      541 aaaggtgcca attttagctt agacggtata ggaaacaata tgttaaatta tattcttttt
      601 agcaatcaag atgctacttt tcaaggtaat ttttctatca ccgatataaa tattgatcct
      661 tttgaaaaac attattatga ttacctttgt aatgaaccat ctacaagcac attacaaact
      721 cgtattaatg taacagcaaa acaagtggaa tatgacaatt ttacattcca agatgtttca
      781 tgcatactta tatatacagg caatacattt aatttattgc aaacaaaact taaaacaatg
      841 aaaggattat ttgccggcga cgtgtatgta acatactatg ataacaatac cacaaaagta
      901 gaagctaatg gggacttcaa aaaagtttca gcaaaagaat tatttaaaac attcaataat
      961 tttgatcaaa cctttttaac agaatctcaa atatcaggca ccatatcaac aaaattcaat
     1021 atgtcaacta tgcttgataa agattacaac ccaatttatt caagtatgaa aatactaagc
     1081 aatgttataa ttgaagatgg aaatatttct aattttgatc catttataga aatgggtaaa
     1141 aaaatgaaag tagaagaatt caaatctgtt tcatttactc gtattgaaaa tatcttaaaa
     1201 atcgaaaacg atactctgta tattccaaac atgcgaattc aaacaaatgc atttgatatg
     1261 aattttgcag gcaaacatgc tattaacaat aatcaattta attattacat tacggtattt
     1321 atgaaaaaaa ctctatcaaa tatgtttaag aaaaaaaatg aaggcgaaga ttttggtgag
     1381 attgaacaaa ataccgacgg aaatttaaaa cttccacttc gcatttatgg aaatcccgat
     1441 aagtattcaa ttgagtatga tttaaaaaca tctaaacaaa atgtaaaaca aactatggaa
     1501 cgacaaaaag aagactggaa agctattctc aataaacaat caaatgaaga aatacaagaa
     1561 aagccaaaaa aacaagaaaa acctattgat tcgggattca aaatagaata tgattaataa
     1621 cagaatacaa aatacatgaa attatatagt gccaaataca aaattaaagt tgctttgttt
     1681 acaattgcaa ttctatttgt tggtgccatt atatatttac tcaatactac cataacaaac
     1741 ataaaacaag aagaacgaaa acaagtatta ctatgggctg aagccgttca aaaacgatat
     1801 acattagttg attatactaa taaactattt gaaaaactgc aagaagaaga acggcgtaaa
     1861 gttgaattat ggtctgacgc acaacatcat ttattatatg aagatgattc ccaatttttg
     1921 acctttttac tcaaaatcat aagtagtaat aaaaacatac ccattatttt aactgatgct
     1981 aaagggaatg ttgtttctag tgtaaatacc gattttgcaa ttccactcaa taaacctatg
     2041 cctgacagta tcaaacaaat tttcacagaa tataatccta tacctatttt atatgacaac
     2101 gaaccaataa actacttgta ttacactgat tcaaaactat ttagtgagct tcaaaatgta
     2161 atgaataata tgattcaatc gtttattgaa gaagtagtta aaaatgcagc ttcggtacct
     2221 gttatcatta ctaataaaga cacaactcaa ataattgcat acgggaacat tcaaaaatat
     2281 aggattgaaa cgaatgaaaa gatttttcaa accattcaag aaatggcttc gagtaatcca
     2341 ccaattccaa tcatattaaa caatagtgtt gttcattata tattttacga agactctaca
     2401 cttataactc gacttcgata ttaccctaca atacttattg gcattgggtt tattgtatta
     2461 ctctttgcat attttgcatt acgaagttcc gaaaaatttg aacaaaatca gttaatggtt
     2521 ggtatgtcaa aagaaactgc acatcaatta ggcacaccta tttcatctct tatggcttgg
     2581 gttgaattac ttaaacaaca aaatatagac aatgacattg taacagaaat caataaagat
     2641 gtaactcgat tgcaaacaat agctgaacgt ttttcaaaaa ttggttctgc tcccaatctc
     2701 aatccacatg tattatacga tacaattcaa aaatctttgg attatataca aatcagaacc
     2761 tctcaaacta tttcatttca tcttcattgc caacaaaaaa atatagtagt tcctctcaat
     2821 atagctttgt ttgaatgggt tatagaaaat atttgtaaaa atgctataga tgcaatgcaa
     2881 ggtaaaggtt cactttctat tactattatc agtcaatcgc catgggtaca tattgatata
     2941 gaagattcgg gtaaaggaat acctcgaaat aaatttaaaa ccatatttaa acccggatat
     3001 acaactaaac aacgtggctg gggattagga ttaagtttag caaaacgaat aattgaagaa
     3061 taccacaatg gtaaaatttt tgtaaaattt tcagaattaa acaaaggaac taccattcga
     3121 ataagtttac caatggctta attcattatg aaaatacgcg atgcttttaa tatattagaa
     3181 atatcggatg atgctagtcc cgaagaaatt aaaaaagcat acagagcaaa agcaaaactg
     3241 tatcatcctg atgtaaataa ttcaaattcg gctcaagctg aatttgaatg tattcaaaat
     3301 gcttacacac ttattcaagc atatattcaa tacagagaat caaaacattc tcattctgaa
     3361 atacaaaaca caaaaatcaa acgtaaacaa aactctaaac agacgaaagc tgcaaaaaaa
     3421 caatggtttg aagctcgaat tcaatatgat ataagcatat tcaaaaaatc caaacaatat
     3481 attctctcat attgttttgc ttttattggg agcatagttg ctcttggaat ttgtattgat
     3541 tatttttctg cacctcaaga aaattatgaa ccaattcaac acatagaaat tccccaaaat
     3601 tttcatgaca ttaaaaatta tgcatggaaa gaattatata ttattgttca agatcgtaga
     3661 atttcaatta ctccattact gttaaaccaa atacaaaaca cttcatacct taaaatatat
     3721 aatacaccat atttgcaaca aattaaacgt attgaagcaa caaatagtca accatataca
     3781 gaaattgaat tttacaattt ttacaatgat gcattcctca tcatagtaat tttactctgt
     3841 ggggggataa gtatgtttgt ttttaacaaa aaatctcttg gatattatag cataattcgg
     3901 ttttacaatc tatattgttt acctcttatt attctatttg ttttattggg tgaagctcga
     3961 atattgcgtt tatttggtat tgtataaaat tatctcgact ttatttaaca gacgaatatt
     4021 gaatatcagt atatgatact tttggtttta aataatatat tttaccgttt gtggttgcac
     4081 aaaatatagt accattaggc aatactacag gggtagaaaa aactttaccg tcaacaaaat
     4141 atttacatat aatttcacca tttatattta atacataaat atttttatca tacgaaccta
     4201 ctactataaa attatcttga gtaattgcag gagaagctac tatccgcttt tttgtggcaa
     4261 tagaatgcaa caattgtcca tcatttttaa gaatataaat atgtttatca aacgaaccaa
     4321 atacaatttt atcgtcaact ttttgaggtg aagcatgtat ttttccattt gttttaaact
     4381 tccattctaa ttttccttgt aaattaattg cgtgtaaata tctgtcgtat gacccaaaaa
     4441 caatagttga atcctttacc tgcaatgctt tagaatgcat aatccaacca tttgtcaaat
     4501 acgatgacac caaattagca gagtcattaa aaatatacat tcgcttgtca ttacccccaa
     4561 cacatatatt tttattactt aaaagttgag ggcttccgtg caccattttt tttgtagtaa
     4621 attgagtata tgtattttct ataaaattat aaaaaatcac tcctttttta ttactgccaa
     4681 atattacacg agaagaatct aattgcaaag cattagtaaa aattgaccca tgtaatttta
     4741 tttgttttaa caaagaacca atctcattaa aaaaatacat attaccatca taagaagcaa
     4801 cactaactat tgtatcagaa acaattgaag gagttgcatg cacccatcct ttggtagaaa
     4861 attcttgaat tttatcgcct actgaattaa aaaaatatat tttttttcta tgattaccaa
     4921 ttacaacatt tccttgtttt gtaactattg gactactata aaaagctcca tgtaattgaa
     4981 ttgcagta
//
LOCUS       DAOTOW010000065         4985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13966_length_4985_cov_4.034280, whole genome shotgun sequence.
ACCESSION   DAOTOW010000065 DAOTOW010000000
VERSION     DAOTOW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13966_length_4985_cov_4.034280"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..872)
                     /locus_tag="P5243_01775"
     CDS             complement(141..872)
                     /locus_tag="P5243_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18205.1"
                     /translation="MKKFVHCIAIAILSVSMTSVFAQETEAPANEGNSCETKLDVGLD
                     IYSSYIWRGLKFGYGPAFQPKVELTSGGFTLGAWGNVNANSGHDAGENPEAFEADLYT
                     SYTFPFGLTLGLNDYYFGGNYLGVSFNNMARTHSIEPFFEYVYDDNLTFYGALMFMEE
                     RVTDFYCQVSYDFDLFNFAIGAGDGQYTMNPVYKNGGEFSFCNLTISKEKTIKVTDSF
                     KIPVTGAVTLNPTNERFFVYVGMSF"
     gene            complement(990..1706)
                     /locus_tag="P5243_01780"
     CDS             complement(990..1706)
                     /locus_tag="P5243_01780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS18206.1"
                     /translation="MNKILHTDTLGLAYTSYFEGNEHAFFTLLSPHFEPDDVAVAHFF
                     RNFNSMPAIEQRAIEECYGKILDVGAGTGSHSVELQKRGFQVTALDISPLACNIMQKR
                     GIIKVLCEDFYTHSYKTYNTILFLMNGIGIVGTLQGFPRFFESLERILEPNGQVLVDS
                     SDLRYLFEEEDGSFAIPLCGYYGELEYTVEFENYTSKPFNWIYVDFDTLASEAYKAGW
                     NCTLVAVGDHYDFLARLTRI"
     gene            complement(1699..2730)
                     /locus_tag="P5243_01785"
     CDS             complement(1699..2730)
                     /locus_tag="P5243_01785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRS18207.1"
                     /translation="MDELLENERGLFEHFKFVIDQGQSMIRVDKFLSDKIDTYSRTKI
                     QQAADNGFLFANGTNIKSNYKIKPGDVITIELDYPKHELEIIPEQIPLDIHYEDEHLI
                     VIHKPPGMVVHPGYGNYTGTMVNALAWHLRDNIQFESNDPRPGLVHRIDKDTSGLLVV
                     AKHEQAKTALAKQFFEKTSERTYIAVVWGIPKERKGTISGYIGRGLKDRKVMTMFPDE
                     THGKWSVTHYTVLQELGYVSVVECKLETGRTHQIRAHFKSIGHPLFNDETYGGNHILR
                     GTTYAKYKQFVTNCFAICPRQALHAKTLGFIHPFTKQFLQFDSPIPSDMEQLIEKWHT
                     YISTRDNDE"
     gene            complement(2743..3894)
                     /locus_tag="P5243_01790"
     CDS             complement(2743..3894)
                     /locus_tag="P5243_01790"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRS18208.1"
                     /translation="MKRVAILGSTGSIGTQALEVISQNPQHFTVEVLTAHNNADLLIE
                     QAIQFQPNTVIISNKEKYLYVQQSLDKYMIKVFAGEEALAQVVEMDSIDIVLGALVGY
                     SGLIPIINALKHGKPVALANKEVLVVAGQLVNKLSHQHNAAILPVDSEHSAIFQCMAG
                     EFEYGNSIEKLFLTASGGPFRGYTQTQLQSVTKQQALKHPNWDMGAKVTIDSASLMNK
                     GLEMIEAKWLFGVDAKQIEIIVHPQSIIHSAVQFSDGSIKAQMGLPDMKLPIQYALSY
                     PQRVPSTFPRFSFWDYPQLTFEKPVIENFRNLHIAFEAIAKSGNMPCIMNAANEVAVH
                     AFLHDKISFLGMSGLIEKTMQHISYIAQPSLDDYIHTHNESIAYAQSLL"
     gene            complement(3922..4812)
                     /locus_tag="P5243_01795"
     CDS             complement(3922..4812)
                     /locus_tag="P5243_01795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS18209.1"
                     /translation="MAKIQTGRKPRTRGKRIEKLKNKYRLIIFNDESFAEIYSFRLTK
                     INLFLLFGFVSIFMVSLVYILIAYTPLKEYVIPDYPKLEEREKIIQNTIKVDSLEHQL
                     KLYDQKLQILHMVINGDEPPQYMNEIPDSADNFSSISFSRSVEDSLLRLEIQEFEALN
                     VNFKPGTITNPKYVNIYFHAPVRGTVSNKYSAEQKHFATDIVAPKNSVIHSVLPGIIV
                     FNSWTIETEYTCIIQHSNNFISVYKHNSKLLKQTGDKVEAGEGIAIIGNTGELTTGPH
                     LHFELWHEGKPVNAEDYIVF"
BASE COUNT         1534 a          840 c          819 g         1792 t
ORIGIN      
        1 aaattttcga ggcttagata ttatattatg ctgttttata atcacataca ctaaacctcg
       61 cttacgcttt taaaaaaaca aaagtagtaa aaagtatatg tgaaaaaata tctatgcatg
      121 tttttttata attatctcaa ttagaatgac attcctacat atacaaaaaa tctttcattt
      181 gtaggattaa gtgttactgc acctgttaca ggtattttaa acgaatcggt aacttttatt
      241 gttttttctt ttgaaatggt aagattacag aaagaaaatt ccccgccatt tttatatacc
      301 ggattcatag tatattgacc atcgcctgct cctatagcaa agttaaataa atcaaaatcg
      361 tatgaaactt gacaatagaa gtcagttact cgttcttcca taaacataag tgctccataa
      421 aatgttaaat tatcatcata cacatattca aaaaatggtt caattgagtg tgtgcgtgcc
      481 atgttgttga aacttacacc taaataattt ccaccaaaat aataatcatt taatcctaat
      541 gtaagtccga aagggaatgt gtatgaagta tataaatcag cttcaaatgc ttcaggattt
      601 tctccggcat cgtgtccgct gtttgcattt acgttacccc atgctcctaa tgtaaaacca
      661 cccgatgtta attctacttt tggttgaaat gcaggtccgt atccgaattt taatccacgc
      721 caaatataac tgctgtaaat atctaaacca acatctaatt tagtttcaca agaatttcct
      781 tcgtttgcgg gagcttctgt ttcttgagca aatacacttg tcatacttac cgaaagtatt
      841 gctattgcta tacaatgtac aaattttttc ataagctata tgttttaaat taaactcaca
      901 caaatataga gcattatttt agttttcaaa atttttttat gatgtattgc agtttttttg
      961 ggttttaatg atacctattt cggtattgat tatattcttg taagtcttgc caaaaaatca
     1021 taatggtcac caactgcaac taacgtacag ttccaacctg ctttgtatgc ttcgcttgcg
     1081 agtgtatcaa agtctacata tatccaatta aatggttttg aagtataatt ttcaaattct
     1141 acagtatatt ctaattctcc gtaataacca cataggggaa ttgcaaatga accatcttct
     1201 tcttcaaaca aatatcgcaa atccgaagaa tctaccaata cttgcccgtt tggttcaagt
     1261 atacgttcca gcgattcaaa aaagcgagga aatccttgta gtgtgcctac tattcctatt
     1321 ccattcatta aaaacagtat agtgttatat gttttatacg aatgtgtata gaaatcttca
     1381 cacagtactt ttattatacc tcgtttttgc ataatattgc aggctaatgg tgatatgtcg
     1441 agtgctgtaa cttgaaaacc acgtttttgt aattccacgc tatgactacc tgtgccggca
     1501 cccacgtcta aaattttacc ataacattct tcaatggcac gttgttctat tgccggcata
     1561 ctattaaaat ttctaaaaaa atgagcaact gcaacatcgt cgggttcaaa gtgtggtgac
     1621 aataaggtaa aaaatgcatg ttcgttacct tcaaagtaac ttgtataggc taatccaaga
     1681 gtatcggtat ggagtatttt attcatcgtt gtcgcgtgtt gaaatatacg tgtgccattt
     1741 ttcaattaat tgttccatat ctgatggtat aggagaatca aattgcaaaa attgtttggt
     1801 aaaggggtgt ataaatccta aagtttttgc atgaagcgcc tgtcgaggac aaatagcaaa
     1861 acaattagtc acaaattgtt tgtatttagc atatgttgtt cctcgtaata tgtgattccc
     1921 tccgtaagtt tcgtcattaa acaacgggtg acctattgat ttaaaatgtg ctcttatttg
     1981 atgtgttctg cctgtttcaa gtttacattc cactaccgat acatatccta gttcttgtaa
     2041 tactgtataa tgcgttacag accatttgcc atgtgtttcg tcagggaaca tagtcattac
     2101 ttttcggtct tttaaccctc gacctatata gcccgaaatg gttccttttc gttctttggg
     2161 aataccccaa actactgcta tatatgtgcg ttcactggtt ttttcaaaaa attgttttgc
     2221 cagtgctgtt tttgcttgtt catgtttagc tactaccaaa agtcccgatg tgtctttgtc
     2281 aattcggtga accaatcccg ggcgtgggtc gttcgattca aattgtatat tatcgcgtaa
     2341 atgccaggcc aaagcattaa ccatagtacc tgtgtaattt ccgtatccgg gatgcacaac
     2401 cattcccgga ggtttgtgta ttactataag atgttcgtct tcgtagtgta tatcaagcgg
     2461 aatttgttcc ggaattattt ctaattcatg ttttggataa tcaagttcta tagtaataac
     2521 atcgcccggt tttattttgt aatttgattt tatattggtg ccgtttgcaa ataaaaatcc
     2581 attgtctgct gcttgttgaa tttttgtacg cgaatatgta tctattttgt ccgataagaa
     2641 tttatcgaca cgaatcattg attgaccttg atcaataaca aatttgaagt gttcaaataa
     2701 tcctcgttcg ttttctaata gttcgtccat tgtaattagc ggttacaaca atgattgtgc
     2761 atatgctata gattcattgt gtgtatgtat ataatcatca agcgaaggct gtgctatata
     2821 tgaaatatgt tgcatagttt tttcaattaa acccgacatt cccaaaaaac ttattttgtc
     2881 atgcaaaaat gcgtgaacag caacttcatt tgcggcattc attatgcaag gcatattacc
     2941 cgattttgca atagcttcaa atgcaatatg taaatttctg aagttttcaa ttaccggttt
     3001 ttcaaaagtt aattgtggat aatcccaaaa actaaagcgt ggaaatgtag atggaactct
     3061 ttgtggatac gacagagcat actgtatagg taatttcata tcaggcaatc ccatttgagc
     3121 ttttatagaa ccatcagaaa attgtactgc cgaatgtata atagattgtg gatgaactat
     3181 aatttcaatt tgtttagcat ctacaccaaa taaccatttt gcttcaatca tttctaagcc
     3241 tttattcatg agcgatgccg aatcaatggt aactttggca cccatatccc aattcggatg
     3301 ttttaatgct tgttgttttg taacactttg taattgagtt tgtgtatagc ctctaaaagg
     3361 tcctcctgat gctgttaaaa acagtttttc aatgctgttg ccatattcaa attcgcctgc
     3421 catacattga aaaatagcag aatgttcaga atctacaggt agtatggcgg cattgtgttg
     3481 gtgcgacagt ttatttacta attgtccggc aactaccaat acttctttgt ttgccagtgc
     3541 tacgggtttt ccgtgtttta aagcatttat aattggtata agtcccgaat atcctactaa
     3601 tgcacccaat acaatgtcta ttgaatccat ttcaaccact tgtgcaagtg cttcttcgcc
     3661 ggcaaatact tttatcatgt atttgtctaa cgattgttga acatacaaat atttttcttt
     3721 gtttgatata attacggtat ttggttgaaa ttgaatagct tgttcaataa gtaaatcagc
     3781 attattatgt gcggtaagta cttcaaccgt aaaatgttga ggattttgtg aaataacttc
     3841 taatgcttgc gttcctatag aacctgtaga acctaaaatt gcaacacgtt tcatgtattg
     3901 aatacgaatt tgtaaaatta tttaaaaaac aatataatct tcagcattta ccggtttgcc
     3961 ttcatgccaa agttcaaaat gaagatgagg acctgtagta agttcccctg tatttcctat
     4021 tattgcaatc ccttctcctg cttctacttt atcaccggtt tgtttcagca gttttgaatt
     4081 gtgtttatat accgaaataa aattgttact gtgttgaatt atacatgtat attcggtttc
     4141 tatagtccat gaattaaata caataattcc cggtagtaca gaatgaatta cactattttt
     4201 gggcgctaca atatcggtag caaaatgttt ttgttcggcc gaatatttat ttgaaaccgt
     4261 accgcgaacg ggtgcgtgaa aatagatatt tacatattta ggattggtta ttgttcccgg
     4321 tttaaaattt acgtttaatg cttcaaattc ttgaatttct aaacgtaaaa gagaatcttc
     4381 aaccgaacgt gaaaatgata ttgatgaaaa attatcggct gaatcaggaa tttcgttcat
     4441 atattgagga ggttcatctc cgtttattac catatgtaga atttgtaatt tttggtcata
     4501 tagttttaat tgatgttcta atgaatcaac ttttatagtg ttttgaataa ttttttctcg
     4561 ttcttctaat tttggataat caggtataac atattctttg agaggggtat atgctataag
     4621 gatatagact aacgaaacca taaatataga aacaaaacca aataataaga aaagattaat
     4681 ttttgttaaa cgaaatgaat aaatttctgc aaatgattca tcattaaaaa taataaggcg
     4741 atacttattt ttaagttttt caattctttt ccctcgtgtt cttggttttc taccggtttg
     4801 aattttcgcc atacagaaag ttttttacaa agatacgtat tttgaataat attgtatacg
     4861 ttagtgtatg aaactacaaa atagtagttg aaaaattatt gtaaacaatc tgaaattaaa
     4921 cgaagtagtt tgcgtttttg tataggttta gaaataatag ctgtgcaacc ggcattaaat
     4981 acttg
//
LOCUS       DAOTOW010000066         4976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_13993_length_4976_cov_3.386100, whole genome shotgun sequence.
ACCESSION   DAOTOW010000066 DAOTOW010000000
VERSION     DAOTOW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13993_length_4976_cov_3.386100"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1464)
                     /locus_tag="P5243_01800"
     CDS             complement(25..1464)
                     /locus_tag="P5243_01800"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRS18210.1"
                     /translation="MCSHIYTVLVAMLLFAYTATAQIDKTMYFMDRLPQANYINPSAY
                     PECTFFMGGLLLPLVGQLPPPIMFAVNTPFDWNDFVFHGRWEYQDSLIHPLHPNANLD
                     DFLKKVHTYNNFSTDFQLNLLYFGFKQGKNFWSLDISNKLYLHWGVPGDLMKFAILGN
                     GEVRDAQFNGLYMNVNLYNQISLGFKRQVSRYFSVGGKLKYLSGIANVNVKKSNLYIK
                     TAANSTYIRISPEYLIQTNAPVEVKLADNGLVEEVNFLEFNSNNIPNEIAKNVLFTGN
                     RGLAMDLGFSKDWNSEFTYYFNIEDFGYIHWKRHTHNFSAVAEEGFEFKGLIEIEDDK
                     RKTIQDMFVPDSLLAKFDFTYTTDSYRTNLPWKFYAGARYKVTPKFYLGGVARLEKMS
                     FGYRPSAALSLNYRPGKFGLFTLSYSYQNYSFYNIGIGSTTRIGPVQWYFVSDNLLGT
                     IFWPHQSRSLSMRMGCNLVFGYKEKEKPQ"
     gene            complement(1476..3689)
                     /locus_tag="P5243_01805"
     CDS             complement(1476..3689)
                     /locus_tag="P5243_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18211.1"
                     /translation="MKYIYPLYKYFTIALIVLLAYSCVKDNFDVREKFSNDIKWNPSI
                     ALPLAYGHLTLGNIVKERKDTIQHISEKTLGYGSQDSDLVIQLRYGLDSARKIEILCL
                     PVMQPYDTTLYLKPIQLLDAHTEFDKKVGELLQDNFSNTDYSDYQNYEAMSPPPLTVT
                     ERTADKVETYSSPTSPFTVIEYAVISEGNMEVICTNNFTVPFTADIAVLTDSAGIRQE
                     ITVFNFSSQWINPGSSMSDVFTFDHTYLGSQLFYEYRNMKFADASNVTVDLNNTIDLD
                     IHLTNLKASQGKAVVPQQTITMDSLVYVTIRDKDESKKIYKIIVDEGLIHYKITSSMQ
                     VATEFFVIFESMKYQNDTVTKNAALNRFHPVEEADWFLQDLEADLTKNPDQPYNSIPI
                     RLGYKVYTGTELMEFGPQQNLNIQISNPDSIVFRYLEGNMGKIEEDVFEDSIDFQIED
                     FLKNFLSGEITFYDPKLRIVYHNPVGIPGKFKMNIVGTNSKGESVSALKENANMFTIH
                     APTCDMVKNGDVIISNIEFNKNSSNIVDFIRLMPNKIAYSGKYLLNFDEPDENSIVNC
                     VSNVGNARLTVEVELPMKLSIKDLVLRQEVPLSQIPEIENITNIESLRLFVYTENQLP
                     LDVKLKITMLDTTLVGAAQELGVLDVIVLESGSTINGKVARNSTNKHTEEIVLTSATD
                     SRLEKFLQSNKLRLEVLVETDQQGNVPVIFYTYYGLKFNLAVDGKFLYEGKLNMK"
     gene            complement(3722..>4976)
                     /locus_tag="P5243_01810"
     CDS             complement(3722..>4976)
                     /locus_tag="P5243_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18212.1"
                     /translation="KSNKLYADFLDDDSKTQLNNQSELLKKCNSLVIQISADHEMFYN
                     NIFINFSAVQEEKPQTIWESYIDSAIICKPKFIDTQTGDITILVQDAHYNLILLNNSG
                     KIQWKKPLKEPIMSEFYLIDYFKNGKTQYLFNTANAIYIVDRLGNFVESYPIQLSSPA
                     TAGIAVFDYEKNKEYRIFVPCADKKVYLYNIKGNKIQDWKFGQTETEVTTPLQHFVAE
                     GKDFIVFADKFNMYIVNRKGETRVTVQEQIQKSAQNSFTFEPGVGKVPARLVTTDVQG
                     NIKFIEFSGKVTTLELSKRSPNHFFEYRDFNNDGSNDFMFVDNQELQIISQNKKLISN
                     FVFQAPITLPPVVYKFSLNTYKIGVVDQKNAKVYVINGNGTLHKGFPLKGTSLFTIGF
                     LYKNSNTFNLIIGGEENFLYNYEIK"
BASE COUNT         1692 a          856 c          667 g         1761 t
ORIGIN      
        1 ttctttctct tctttctttt tcttttattg tggtttttct ttttctttgt atccaaacac
       61 caaattgcat cccattctca tactaagcga acgactttga tgaggccaga atattgttcc
      121 taataaatta tctgaaacaa aataccattg aaccggacca attcgtgtag tagaacctat
      181 accaatattg tagaaactat aattttgata cgaataactt aaggtaaaca atccaaattt
      241 accgggtcta taattaagcg aaagtgcagc tgaaggtcta tatccaaaac tcattttttc
      301 aagacgagca actcctccca gataaaattt aggcgtaact ttatagcgag ctcctgcata
      361 aaatttccaa ggtagatttg ttctatagct atctgtagta tatgtaaaat caaattttgc
      421 aagaagacta tcaggcacaa acatatcttg aatagtctta cgtttatcat cttctatttc
      481 tattaatcct ttaaattcaa aaccttcttc agctacagcc gaaaaattat gagtgtgtcg
      541 tttccaatgt atatatccaa aatcttcaat attaaaataa tacgtaaatt cactattcca
      601 gtcctttgaa aatcctaaat ccattgcaag tcctctgtta cctgtaaaaa gaacattttt
      661 tgcaatttca ttcggaatat tattcgaatt aaattcaaga aaatttacct cttcaactaa
      721 tccattatca gcaagtttta cttcaacagg tgcattagtt tgaatcaaat attcaggcga
      781 aatccttata tatgttgaat ttgccgctgt ttttatatat agatttgatt tttttacatt
      841 tacatttgct atacccgata aatacttcaa tttacctcct acactaaaat aacgagaaac
      901 ttgtcgttta aaacctaatg atatttgatt atataaatta acattcatat ataaaccatt
      961 aaattgagca tcgcgtactt cgccattacc caaaattgcg aatttcatta aatcaccggg
     1021 tacgccccaa tgtaaataca atttatttga tatatcaagc gaccaaaaat tttttccttg
     1081 tttgaaccca aaatataata gattcaactg aaaatcggtt gaaaagttat tgtatgtatg
     1141 aacctttttt agaaaatcat ccaaatttgc atttggatgt aatgggtgaa ttagagaatc
     1201 ttgatattcc catctgccat gaaacacaaa atcattccaa tcaaatggag tatttacagc
     1261 aaacataatt ggtggaggta attgtcccac taaaggtaat aataaacctc ccataaaaaa
     1321 tgtacattcg ggatatgctg aaggatttat atagtttgct tgcggcaacc tatccataaa
     1381 atacatagtt ttatcaattt gagctgttgc ggtatacgca aaaagcaaca ttgcaactag
     1441 tactgtatat atatgtgagc acatcaactt catcattatt tcatattcaa ttttccttcg
     1501 tataaaaatt tgccatctac agccaaatta aattttaacc cataataggt ataaaaaata
     1561 accggaacat ttccttgctg atctgtttca accaaaactt ctaaacgcag tttatttgat
     1621 tgcaaaaatt tttctaaccg tgaatctgtt gccgaagtta acactatttc ttcggtatgt
     1681 ttatttgtgg aattacgcgc aactttacca ttaatggtac ttcctgactc caatacaatt
     1741 acatctaata ctcctaactc ttgagctgca ccaactaatg ttgtatcgag catggtaatt
     1801 ttcaatttta catcaagtgg caattgattt tcggtataaa caaacaaacg taaactttca
     1861 atattagtaa tattttctat ttcaggaatt tgtgacaatg gaacctcttg acgtaaaact
     1921 aaatctttaa ttgagagttt cataggtaat tcaacttcca ctgtaagtcg agcattccct
     1981 acgttggata cacagtttac gatagaattt tcatcaggtt catcaaaatt taacaaatac
     2041 ttaccggaat atgctatttt attgggcatc aaccgaataa aatcgacaat atttgatgaa
     2101 ttcttattaa attcaatatt tgatattatt acatcaccat tttttaccat atcacatgtt
     2161 ggagcatgaa tagtaaacat gtttgcattt tctttcaaag cacttacact ttcaccttta
     2221 gaatttgtac ccactatatt cattttaaat tttcccggaa taccaacagg attatggtaa
     2281 actatacgta atttagggtc ataaaaagta atttcgcctg ataaaaagtt tttaagaaaa
     2341 tcttctatct gaaaatcaat actatcttca aatacatctt cttctatttt acccatattt
     2401 ccttccaaat agcgaaatac aatactatca ggattcgaaa tctgaatatt taaattttgt
     2461 tgggggccaa actccattaa ttctgttccg gtatatactt tatatcctaa tcggatagga
     2521 atagagttat aaggttgatc aggattttta gttaaatcag cttctaaatc ttgcaaaaac
     2581 cagtcggctt cttctacagg gtgaaacctg ttgagtgcag cattttttgt aacagtatca
     2641 ttttggtatt tcattgattc aaaaatcaca aaaaattcgg tagcaacctg catactcgaa
     2701 gtaattttat agtgaatcaa cccttcatct acaattattt tatatatttt tttggattca
     2761 tctttatcac gtatagttac atataccaat gaatccatag ttattgtttg ttgaggcact
     2821 acagccttac cttgcgaagc ctttaaattt gttaaatgaa tatctaaatc aatcgtgtta
     2881 tttaaatcta ccgtaacatt tgacgcatcg gcaaacttca tatttctata ctcataaaaa
     2941 agttgagacc ccaaataggt atgatcaaac gtaaacacat cactcattga actacccgga
     3001 ttaatccatt gcgaactaaa gttaaaaaca gtaatctctt gtctaattcc ggcagaatcg
     3061 gttaacacgg caatatctgc agtaaatggt acagtaaaat tattggtaca aattacctcc
     3121 atatttcctt cactaattac agcatattct ataactgtaa aaggagatgt tggagaacta
     3181 taggtttcaa ccttatcggc agtacgttca gtaacagtaa gtggtggtgg cgacattgcc
     3241 tcataattct gataatcaga ataatcggtg tttgaaaaat tatcttgtaa taattcacct
     3301 acttttttat caaattcggt atgagcatct aataattgta taggttttaa atataaagta
     3361 gtatcgtacg gttgcattac cggcaaacaa agaatttcaa ttttacgagc agaatcaagt
     3421 ccatagcgaa gctgaataac taaatcggaa tcttgtgaac catatcctaa tgttttttcg
     3481 ctaatatgct gaatagtatc ttttctttct tttacaatat tacctaatgt aagatgacca
     3541 taggctaatg gtaaagcaat tgatggattc catttaatat cattggaaaa tttttcacgg
     3601 acatcaaagt tatctttcac acacgaatat gctaataaaa ctattagtgc aattgtgaaa
     3661 tacttataaa gtgggtatat atatttcatg catataaata tttttactaa tatacatcaa
     3721 tttatttaat ttcatagtta taaagaaaat tttcttcacc ccctatgata agattaaatg
     3781 tatttgagtt tttatacaaa aaaccaatag taaataacga tgtacctttg agagggaacc
     3841 ctttatgcaa ggtaccatta ccatttataa catatacttt tgcattcttt tgatctacta
     3901 ctcctatttt gtatgtattt aacgaaaatt tatatacaac cggaggaagt gttataggag
     3961 cctggaatac aaaattactt atcaactttt tattttgcga aataatttgc aactcctgat
     4021 tatcaacaaa cataaaatca ttacttccat cattattaaa atcacgatat tcaaaaaaat
     4081 gatttggtga acgtttggat aactccaatg tagtaacttt tccggaaaat tctataaatt
     4141 taatattccc ttgaacatcg gtagttacca aacgtgcagg cactttacca actccgggtt
     4201 caaaagtaaa agaattttga gccgattttt gaatttgctc ttgaacagta actcttgttt
     4261 ctccttttct atttacaata tacatattaa atttatcggc aaatacaata aaatctttac
     4321 cttcagcaac aaaatgctgt aaaggagttg ttacttctgt ttctgtttga ccaaacttcc
     4381 aatcttgaat cttattgcct tttatattat acagatacac ttttttatcg gcacagggca
     4441 caaatattct atattcttta tttttttcat aatcaaatac agcaatgcct gcggtagccg
     4501 gcgatgaaag ttgtattgga tatgattcaa caaaattacc taatctgtca actatataaa
     4561 tagcattagc cgtattaaac aaatattgcg ttttgccgtt tttaaaataa tcaataagat
     4621 aaaattcact cataataggt tctttgagtg gttttttcca ctgaatttta ccactattat
     4681 taagtaatat aagattataa tgtgcatctt gtaccaaaat tgtaatgtca cctgtttgag
     4741 tgtcaataaa tttaggttta caaataattg cagaatcaat ataactttcc caaatagttt
     4801 gaggtttttc ttcttgaact gcactaaagt ttataaaaat attattgtaa aacatttcat
     4861 ggtcggcaga tatttgaata acaagcgaat tacatttttt taataactca ctttgattat
     4921 ttaattgtgt ctttgaatca tcgtcaagaa aatcggcata taatttattt gatttt
//
LOCUS       DAOTOW010000067         4965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14023_length_4965_cov_2.825051, whole genome shotgun sequence.
ACCESSION   DAOTOW010000067 DAOTOW010000000
VERSION     DAOTOW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14023_length_4965_cov_2.825051"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="P5243_01815"
     CDS             <1..1165
                     /locus_tag="P5243_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRS18213.1"
                     /translation="FFHKGNKGNHDAWDSKCFDYGKNEVLHFLLSNCKYWITEFNFDG
                     YRFDGVTSMLYWDHGLGRSFDSIERYYDGGQDGDAIMYLILANKVIHECKPTAITIAE
                     DMSGMPGLAAPLKYGGYGFDYRLSMGVPDFWIKLIKEKRDEDWHVGMMFHELTQARFD
                     EKVISYAESHDQALVGDKTIAFRLMDKEMYFSMHVAYPSLIIDRGMALHKIIRLITLS
                     CAQNGYLNFMGNEFGHPEWIDFPREGNNWSYHYARRQWSLKKDENLRYKFLGKFDEDM
                     IALVRNTKDFFQNRPRFIHENIQDQIIVFERAGLLFVFSFNPIKSFPDYGIAIPQGEY
                     TVCLTVDSLKYGGLNRIDETMTYISQPIQNETYAEHQLKLYIPTQTAIVLQKK"
     gene            1260..3209
                     /locus_tag="P5243_01820"
     CDS             1260..3209
                     /locus_tag="P5243_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRS18214.1"
                     /translation="MGYLEFTKQEVTNLQESLKREFLRTNRAGSYASSTIINCNTRKY
                     HGLLVCPLKQIDGDNYVLLSSLDETVIQHDTPFHLAIHKYISDYNPLGHKYIESFQND
                     PTPTIIYRVGGVVLQKEMLLVEEEERVLIKYTLIKATSQTTLQLRPFLAFRKVHELTH
                     ANLVANTRHLQVENGIASKLYEEFPYLYMQLSKKNEFVTAPDWYNNFEYAKERERGFA
                     CHEDLFTPGYFEFDIKQGESVVFSAGIQETKTKSLSSVYNREIEKRTPRSNFENCLIN
                     AAQQFISRSGNKTKIVASFPWPGYWGRDSMIALPGLTLAVNDIKTCEDVLLSFSEEID
                     GFVYRNKGNIEHSNVESIDTALWFIRSVQQYNWNTKDFAKTKKLFAKKVEEILTTYML
                     GENNNVVMHDNGLLYLPFEHKALTWVDAKIDGRPIVPRWGYVVEINALWYNAICFALE
                     LITTKSFQDTWKHIPAKIEQSFIEIFWDEDKGYLADYCVNDYKDFSVRPSQIFAASLP
                     FSVLDDVQKHAVLDVVRRELLTPRGIRTLSPKNPQYIGEVKGTIQERDMAYHQGGVLV
                     WLLAPYAEAYLKLHGRSGVTEIKRIYYGMETLMKSHGIGTISEVYDGNPPHEGRGAIS
                     QAWSVAAVLRLKKLIENFEADEQRK"
     gene            3223..4530
                     /locus_tag="P5243_01825"
     CDS             3223..4530
                     /locus_tag="P5243_01825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS18215.1"
                     /translation="MRVLMFGWEFPPHISGGLGTACYGLTKAMAKQNLEIIFVVPRAW
                     GDEDQSTMQLVGANNIRVRVNRYQFSESDVANSTDLQYVEIGANLIPYVTIEEYYDIV
                     NNKVTVKKQLKKHVKTKELDTISFVGGYGESLLHEVSNYAFIAGEIAKKYDFDIIHAH
                     DWLTYDAGIAAKHVSGKPLVIHVHATEFDRSGMHVNQYVYDIERRGMHMADEVITVSN
                     LTRSTVLNHYGVPEHKVTTVYNGVDSVQNEKEKFFEKHIDDKIVTFLGRITYQKGPEY
                     FLEAAAKVLQRKSNVKFVMAGSGDMLEKMMWRAAELGISKNFYFTGFLRGDDVIHMLS
                     ISDVYVMPSVSEPFGISPLEAMRSNVPVIISKQSGVSEVLQHAIKIDFWDVDSMADAI
                     YGIISYDGLRDMFSSHAKAEVETIKWDTAAAKIKDIYTKTLSK"
     gene            4536..>4965
                     /locus_tag="P5243_01830"
     CDS             4536..>4965
                     /locus_tag="P5243_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS18216.1"
                     /translation="MKNICLLFQVNHPFQLRSYRFFDIGHDHYYYDDFSNKSNMLKIA
                     KRCYLPMNDILLSLIKTYKGMFKVNFSISGSAIEHFEMYAPEIIESFKLLAKTGAVEF
                     TAETYAHSLVSVKSPEEFKTQVEKHANKIKELFGKKPKVFS"
BASE COUNT         1771 a          633 c          943 g         1618 t
ORIGIN      
        1 gttttttcat aaagggaaca aaggtaatca tgatgcatgg gattcaaaat gttttgatta
       61 tggtaaaaac gaagttttac atttcttgct ttcgaattgt aaatattgga taacagaatt
      121 caattttgac ggatatcgtt ttgatggtgt taccagtatg ttatattggg atcatggttt
      181 aggaagatca tttgattcta ttgaacgata ttatgatggt gggcaagatg gcgatgcgat
      241 tatgtatctt attttggcga ataaagttat ccatgaatgt aaaccaacag caataacaat
      301 agcagaagat atgagtggaa tgccaggttt ggctgcaccg ttaaaatatg gcggatatgg
      361 ttttgattat cgcctttcta tgggagttcc cgatttttgg ataaaattaa taaaagaaaa
      421 acgagacgaa gattggcatg taggaatgat gtttcatgaa ttaactcaag ctcgttttga
      481 tgaaaaagta atttcatacg cagaatcaca cgatcaagcc ttggttggag ataaaactat
      541 tgcatttcga ttaatggata aagaaatgta ttttagtatg catgttgcat atcctagttt
      601 aattatagac agaggaatgg cattgcacaa aataattcgt ttaatcacct tgtcttgtgc
      661 tcaaaatggg tatctaaatt tcatgggtaa tgaatttgga catcccgaat ggattgattt
      721 tccgcgtgaa ggaaataatt ggtcttatca ttatgctcgt cgtcagtgga gtttaaaaaa
      781 agatgaaaat ctacgataca aatttttagg taaatttgac gaggatatga ttgcgttagt
      841 tagaaatact aaagattttt ttcaaaatcg tccacgattt attcatgaaa atattcagga
      901 tcaaattatt gtgtttgagc gagctggatt attatttgtt ttttcattta atccgattaa
      961 atcgtttcct gattatggaa ttgctattcc acaaggtgaa tatacagtat gtttaacagt
     1021 tgattcatta aaatacggag gtttaaatag aattgatgaa acaatgacgt atatttctca
     1081 accaattcaa aacgagacat atgccgaaca tcagttaaaa ttgtatattc caactcaaac
     1141 agctattgta ttacaaaaaa aatgatgttt tttatataaa ttacgtaaat tagtatgtat
     1201 attcagtcaa tagtatatat ttatttatat ttttgcattg aactaaaaaa attccactaa
     1261 tgggttactt agagtttact aaacaggaag ttacaaattt acaagaatca cttaagcgtg
     1321 aattcttgag aactaataga gcaggttctt acgcaagttc tactataata aattgtaata
     1381 cacgtaaata tcacggctta ttagtttgtc cacttaagca aatagatgga gataattatg
     1441 tattattatc atcacttgat gaaactgtaa ttcagcatga tactccgttt catttagcaa
     1501 ttcataaata tatttcagat tacaatcctt taggacataa atatatagaa tcatttcaaa
     1561 acgatcctac gcccaccatt atatatagag taggaggagt ggttttgcaa aaagaaatgt
     1621 tgttggttga agaagaagag cgtgtgttaa taaaatatac attgataaaa gcaacatcac
     1681 aaacaacatt gcaattacga ccttttttag catttagaaa agttcatgag ttaacacatg
     1741 caaatcttgt tgctaatact cgtcatttgc aagtagagaa tggtatagct tcaaaattat
     1801 atgaagaatt tccgtatttg tacatgcagt tatctaagaa aaatgaattt gtaacagcac
     1861 cggattggta caataatttt gaatatgcaa aagaacgaga acgaggattt gcatgtcatg
     1921 aagatttatt tactccgggg tattttgaat ttgatataaa acaaggagaa tctgttgtgt
     1981 tttcggcagg tattcaagaa acaaagacga aatcattatc aagtgtttac aatagagaaa
     2041 tagaaaaacg aactccacga agtaatttcg aaaattgttt aatcaatgct gcacaacaat
     2101 ttatatcaag aagtggtaat aaaacaaaaa tagttgcaag ttttccttgg cccggatatt
     2161 gggggcgaga cagtatgatt gctcttccgg gacttacttt ggctgtaaat gatattaaaa
     2221 cttgtgaaga tgttttatta tcattttcag aagaaataga tggttttgta tatagaaata
     2281 aaggaaatat tgaacactca aatgtagaat caatagatac tgcactttgg tttatacgtt
     2341 cggtacaaca atataattgg aatacaaaag attttgctaa aactaaaaaa ttatttgcaa
     2401 aaaaagtaga agaaattctt actacgtata tgttgggtga aaacaataat gtagtaatgc
     2461 atgataatgg gttgttgtat ttgccttttg aacacaaagc gttaacctgg gtagatgcaa
     2521 aaattgacgg acgaccaatt gtgccacgtt ggggatatgt tgtggaaatt aatgcattat
     2581 ggtacaatgc aatatgtttt gcattggaat taattacaac aaagtcgttt caagatacat
     2641 ggaaacacat acctgctaaa atagaacaat catttataga aattttctgg gatgaagaca
     2701 aaggatattt agcagattat tgtgtaaatg actataaaga ctttagtgtt agaccaagtc
     2761 aaatatttgc agcatcttta cctttctctg ttttagatga tgttcagaaa catgcagtac
     2821 ttgatgtggt aagaagagaa ttgttaaccc ctcgtggaat tagaacgtta tcacctaaaa
     2881 atccacaata tataggagag gtaaaaggaa ctattcaaga aagagatatg gcttatcatc
     2941 aaggaggtgt tttagtgtgg ttacttgcac catatgccga agcatatctt aaattacacg
     3001 ggcgttccgg tgtaacagaa ataaaaagga tatattatgg catggaaaca ttaatgaaaa
     3061 gtcatggtat aggaacaatt tcagaggtgt atgatggaaa tcctcctcat gaaggtcgtg
     3121 gagcaatatc gcaagcctgg tctgtagctg ctgtattacg actaaagaaa ttaattgaaa
     3181 attttgaagc tgatgaacaa cgcaaataac actatataaa ctatgagagt actaatgttt
     3241 ggatgggagt ttcctcctca tatttcgggc ggtttgggta ctgcatgtta tggattaaca
     3301 aaagctatgg ctaaacaaaa tttagaaata atttttgttg tgccgcgtgc atggggcgat
     3361 gaagatcaaa gtactatgca attagttggt gcaaataaca tacgagtacg tgtaaataga
     3421 tatcaatttt ctgaatcaga tgttgcaaat tctactgatt tacagtatgt tgaaattgga
     3481 gcaaatttaa ttccttatgt tacaatagaa gagtattatg atattgtaaa taataaggta
     3541 actgtaaaaa aacaattaaa aaagcatgta aaaactaaag aacttgatac aataagtttt
     3601 gtaggaggtt atggagaaag tttgcttcac gaagtaagta attatgcttt tatagcagga
     3661 gaaattgcca aaaaatatga ttttgatata atacatgctc atgattggtt aacatatgat
     3721 gccggtattg ctgcaaaaca tgtgtcaggt aaaccattgg taatacatgt acatgctact
     3781 gaatttgacc gtagtggaat gcatgtaaat caatatgtat atgatataga gcgcagaggt
     3841 atgcatatgg cagatgaggt aattaccgtg agtaatttaa ccagaagtac tgttcttaat
     3901 cattatggtg ttccggaaca taaagttact acggtatata atggtgtaga ttctgttcaa
     3961 aatgaaaagg agaaattttt tgaaaaacat attgacgata aaatagttac ttttttgggg
     4021 cgaataacgt atcaaaaagg tccagaatat tttttggaag ctgctgctaa agtattacaa
     4081 cgcaaaagta atgttaagtt tgtaatggca ggaagtggag atatgctcga aaaaatgatg
     4141 tggagagcag ccgaattagg tatttctaaa aatttctatt ttaccggttt tttacgaggt
     4201 gatgatgtta tacatatgtt atctataagt gatgtgtatg taatgccttc tgtttcagaa
     4261 ccatttggga tttcaccatt agaagcaatg cgatctaatg tgcctgtaat tatttctaaa
     4321 caatctggag tttcagaagt tttacaacat gctattaaaa ttgatttttg ggatgtagat
     4381 tctatggccg atgctattta tggaattatt tcatatgacg gattgcgaga tatgtttagt
     4441 agtcatgcaa aagccgaagt tgaaactata aaatgggata ctgctgctgc caaaataaaa
     4501 gatatttata ctaaaacact ttcaaaataa atacaatgaa aaatatttgt ttgctttttc
     4561 aggttaatca tccttttcaa ttaagatcgt ataggttttt tgatataggt cacgatcatt
     4621 attattatga tgatttttca aacaaatcaa atatgttgaa aatagcaaaa cgatgctatt
     4681 tgccaatgaa tgatatttta ctttcattga ttaaaacata taaaggtatg tttaaagtta
     4741 acttttctat ttctggtagc gcaattgaac attttgaaat gtatgcaccc gaaattatag
     4801 aaagttttaa attattggca aaaaccggtg ctgtagaatt tactgctgaa acatatgctc
     4861 attctttagt ttctgtaaaa tcgcccgaag aatttaaaac acaggtagaa aagcatgcaa
     4921 ataaaattaa agaattattt ggaaaaaaac ctaaagtatt ttcaa
//
LOCUS       DAOTOW010000068         4922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14170_length_4922_cov_3.265872, whole genome shotgun sequence.
ACCESSION   DAOTOW010000068 DAOTOW010000000
VERSION     DAOTOW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14170_length_4922_cov_3.265872"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1491)
                     /gene="creD"
                     /locus_tag="P5243_01835"
     CDS             complement(142..1491)
                     /gene="creD"
                     /locus_tag="P5243_01835"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRS18217.1"
                     /translation="MQPESQTTLEKVNSKVRNSLTFRVITIVILILLLLIPVSMIQSL
                     ISEREFRQQEATTEVSSKWANEQIVTGPILTVPYKTFVRVQQNNGMGDKLVESIEYAH
                     FLPNTLQIHSIVKPEVRKRGIFKVIVYTTQLTVSGDFVEPSFKEWDISPEHILWKDAF
                     VSIGMTDLRGIKEQIQLQWNQQKLLFEPGVQTNDVIESGLSCKLTRDETSIPASSFSY
                     TIQLNGSSKLQFTPIGKTTTVNMQAKWGDPKFDGAFLPDTKTITKDSVTASWKVLHVN
                     RSYPQQFKGSVQNRIQESAFGVELKVPVDEYQKSMRSAKYAALFITLTFLIFFFIEVL
                     HSVRIHPMQYIIVGLALCVFYTLLIAISEHITFAWSYLIASFSIITLIGIYAHYMFKH
                     IQISTIFVLLLSLLYGFIFTIIQSEDYALLMGSVGLFVVLAIVMYLSRKIDWYSIHK"
     gene            1642..2412
                     /locus_tag="P5243_01840"
     CDS             1642..2412
                     /locus_tag="P5243_01840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS18218.1"
                     /translation="MPTFLFDDIIFGPVQSRRMGVSLGVNLLPKTIKYCNFNCIYCEC
                     GFTPLANKEISHLLPSRQDVAKHLTSYLQNNTIKPDAITFAGNGEPTIHPDFCEIIND
                     VIAIRNEFAPKTEIVVLTNATQIHKPSILQALEKVDRCMFKLDSAIESTIRAINQPGK
                     QLNVQDFIKHISLFKGKKIIQTLCISGEHNGVQISNITDTEINALIEVYKKINPDEVV
                     LYTIQRDTPIETLQKISKETLEKFAKKVELAGIKTIVS"
     gene            2420..3451
                     /locus_tag="P5243_01845"
     CDS             2420..3451
                     /locus_tag="P5243_01845"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS18219.1"
                     /translation="MEHRIADIQNKTVIIAPLDWGLGHATRCIPIIKALLQNKNTCII
                     ATSGRSLELLKQEFPSCIHEEIPSYGITYSKSAFGLFFKLLLQIPKSLIAKNKESKVA
                     DDLVKKYSVDYIISDNRFGMHSKLCTSIFITHQIRIRLPLIIRWLEYPFFLVNKQMIS
                     KFDECWIPDYPGTKNLSGILSHSWKVPHSYYIGPLTGRESTECITTFDVVAILSGPEP
                     QRTIFENKLRSILPQLPYKSCLVRGVLGTKNYSAQVGNCTIQTFATNSEINKLLCGSK
                     LIISRAGYSSIMDYEKLGIKAILVPTPGQSEQEYLGRYLQHKPNYWCISQSKLTKKLP
                     EIIKEILGK"
     gene            complement(3458..>4922)
                     /locus_tag="P5243_01850"
     CDS             complement(3458..>4922)
                     /locus_tag="P5243_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS18220.1"
                     /translation="PKGKHVIHMQVGDFQGNWSVPISDSLVKLPYPIAYFTIDTIQGC
                     DSLSVQFTNKSIDGDIYTWNFGDGNSSNDLNPKHTYLQSGVYSVSLQAIDTTIHKDST
                     KVQFNLIQIYDSPQVKFIVPSVTICDGDSVEISVDNTWETYTWNTSSSASSIIVKESG
                     VYSVQITDIHSCFAKDSIEMTRVAKPIVFIGADTAICKGNAVVLDAQNLGATYIWNTG
                     AITQTIVADTTGIYAVEVINQNNCSVSDTIQFIVHDVPAISLGTDTAICKGNTIELGP
                     FDDANTYVWNTGDRTNRLILSESQQYSVIATNEYNCVTKDTILVTVQDLPKVFLGNDT
                     VIKQNSELVLAGPQNMKHYMWSNQEISQSIRIVLTNADLLPQEYVLQVTDYNGCSNAD
                     TILITVTADNGIQFSQKYVKVYPNPVVDVLYIDMPQTLTGIEMVKIINEQGQIVYSTI
                     PLVHSSIMVPAYTMVSGVYTVIVVSKEKVYATKILKK"
BASE COUNT         1745 a          820 c          704 g         1653 t
ORIGIN      
        1 tattttttag aaagtctaaa aatacaaaaa aacaatgtga aatgtgtgat tgttgaaaac
       61 tattccaata gcattaaagt atttaaatat ataatatata ttaaataatt atatatcaat
      121 atttaagtaa atactaatta tctatttatg tatagaatac caatcaattt ttcttgacaa
      181 atacattact atagccaata ccacaaacag ccctacactt cccattaata atgcataatc
      241 ttcggattga attatagtaa aaataaatcc atacaatagt gataataaca atacaaaaat
      301 tgtagagatt tgaatatgct taaacatata atgagcatat atgccaatta gagtaattat
      361 agaaaacgaa gcaataagat aactccatgc aaatgtaata tgttcagaaa tagcaataag
      421 taatgtataa aaaacgcata atgctaatcc tacaataata tattgcattg ggtgaattct
      481 tacagaatgc agcacttcaa taaagaaaaa tatcaaaaat gtaagtgtta taaaaagtgc
      541 cgcatattta gccgatcgca tactcttttg atattcatca accggaactt ttaattctac
      601 gccaaaagca gattcttgaa tacggttctg taccgaacct ttaaactgtt gtggatatga
      661 tctgtttaca tgtaatactt tccacgatgc ggttacagaa tcttttgtaa tggtcttggt
      721 gtcaggcaaa aatgctccat caaacttcgg atcaccccat ttcgcttgca tattaacagt
      781 tgtagttttg cctattgggg taaattgtaa ttttgaactt ccgtttaatt gaatagtata
      841 tgaaaaagat gaggccggaa tagatgtttc atcacgtgtc aatttacaac tcaatccact
      901 ttctattaca tcattggttt gcacacccgg ttcaaaaagt aatttttgct gattccattg
      961 tagttgaatt tgttctttaa tccctcgtaa atctgtcatt cctattgaaa caaacgcatc
     1021 tttccaaaga atatgttcag gagaaatatc ccactctttg aaacttggtt ctacaaaatc
     1081 gccacttaca gtcaactgcg ttgtatatac tattacctta aaaattccac gtttacgaac
     1141 ttcgggtttt actatactat gtatttgtaa agtatttggt aaaaaatgag catattctat
     1201 tgattccacc aatttatcgc ccataccatt gttttgttgt actcttacaa aagttttata
     1261 cggcactgta agtattggcc ctgtaacaat ctgttcattt gcccatttag aacttacttc
     1321 tgttgttgct tcttgttgcc taaattcacg ttcacttatt aaactttgaa tcattgatac
     1381 cggaataagc agtaataaaa ttaaaattac aatagttatt actctaaaag taagtgaatt
     1441 acgaactttt gaatttactt tttctaatgt tgtttgcgat tcgggttgca tagcgcttag
     1501 tttttatttg tttaaaatct acaaaatcga aatacagaac gagataaaga tatatatatt
     1561 attacatttt ttttattaaa aaacttataa aacacatcaa taattatact tttgcacgtc
     1621 taaaggcaaa aaaaataaac tatgccaaca tttttatttg atgatatcat tttcggacct
     1681 gttcagagca gaagaatggg agtttcatta ggtgtgaatt tattacctaa aactataaaa
     1741 tattgtaatt ttaattgtat ctattgtgaa tgcgggttta caccacttgc aaataaagaa
     1801 atttctcatc ttttaccttc tcgacaagat gttgcgaaac atttaacctc atatttgcaa
     1861 aacaacacaa taaaaccaga tgctataaca tttgcaggaa acggagaacc aacaattcat
     1921 cctgattttt gcgaaattat aaacgatgtt atagctattc gaaatgaatt cgccccaaaa
     1981 acagaaattg tagtgcttac caatgctaca caaattcata aaccaagcat tttacaagca
     2041 cttgaaaaag ttgacagatg tatgtttaaa cttgattctg ctatagaatc tacaattcgt
     2101 gctattaatc aaccgggcaa acaacttaat gttcaagatt ttattaaaca tatatcatta
     2161 ttcaagggaa aaaaaataat tcaaactctt tgtatatcag gcgaacacaa tggggttcaa
     2221 atatctaata ttaccgatac cgaaataaat gcacttatag aagtttacaa aaaaattaac
     2281 cctgatgaag ttgtactgta tacaattcaa cgtgatacac caatagaaac tttacaaaaa
     2341 attagtaaag aaacattaga aaaattcgca aaaaaagtag aacttgcagg tataaaaact
     2401 atagtatcat agtaactata tggaacatag aatagcagac atacaaaaca aaaccgtaat
     2461 tattgctccc ttggattggg gattaggaca tgctacacgg tgtattccaa ttataaaagc
     2521 acttttacaa aataaaaata catgtataat tgccacatcc ggacgttctc ttgaactttt
     2581 gaaacaagaa ttcccttcat gtattcacga agaaattcct tcatacggca ttacatacag
     2641 taaaagtgcg tttgggttat tttttaaact attgttacaa attccaaagt ctcttattgc
     2701 taaaaacaaa gaaagtaaag ttgctgatga ccttgtaaaa aaatattcgg tagattatat
     2761 tatttctgat aatcggtttg gaatgcatag taagttatgt acttctattt ttattactca
     2821 tcaaatacgc attcgattac cattgataat tcgatggtta gaatatcctt tctttttagt
     2881 taataagcaa atgatttcaa aattcgatga atgttggatt cctgactatc cgggcacaaa
     2941 aaatttatcg ggaatattat cacacagttg gaaagttccg cattcttatt atataggccc
     3001 actcactgga cgcgaatcta cagaatgtat taccacattt gatgttgtag caattttatc
     3061 gggtcctgaa cctcaacgta ctatttttga aaacaaatta cgttctatat tacctcaact
     3121 tccctataaa agttgcttgg ttcgtggcgt attaggaaca aaaaattact cagctcaagt
     3181 tggaaactgt acaatacaaa catttgcaac caattcagaa attaataaac tcttatgcgg
     3241 ttcaaaactt attatttcac gtgcaggata cagttcaatt atggactacg aaaaattagg
     3301 tattaaagct attttagttc ctacaccagg gcaaagtgaa caagaatatt taggacgata
     3361 tttacaacat aaacctaatt attggtgtat cagtcaatct aaattaacaa aaaaattacc
     3421 tgaaataatt aaagaaatat taggaaaata agaatattta ctttttaagt attttagtgg
     3481 catatacttt ttctttactt acaactatta cagtataaac tccgcttacc atagtatatg
     3541 caggcaccat tatagaagaa tgtaccaacg gaatagtact atatacaatt tgtccttgtt
     3601 cgtttattat ttttaccatt tcgattcccg taagtgtttg aggcatatca atatacagaa
     3661 catccacaac aggattagga tatactttta catatttctg tgaaaactga ataccattat
     3721 cagctgttac tgtaatcaat attgtatcgg cattactaca accgttataa tcggttactt
     3781 gcaatacata ttcttgaggc aacaaatccg cattagttag aacaattcgt atagattgag
     3841 aaatctcctg attactccac atataatgtt tcatattttg gggtccggca agcacaagtt
     3901 cactattttg tttaattaca gtatcattac ctaaaaatac tttaggtaaa tcctgtacgg
     3961 taacaagaat tgtatctttt gtaacacaat tatattcatt tgttgctatt acagaatatt
     4021 gttgcgattc tgaaagtata agcctatttg ttctatcgcc ggtattccat acataggtat
     4081 tggcatcatc aaaaggacct aattcaatag tattaccttt acatattgcc gtatctgtac
     4141 ccaaagatat agcaggaaca tcatgaacta taaattgtat tgtatcggat actgaacaat
     4201 tattttgatt tataacttca acagcatata ttcccgtagt gtcagcaact attgtttgtg
     4261 taatagctcc cgtattccat atgtatgtag ctcctaaatt ttgagcatct aataccacag
     4321 cattaccttt acatattgcc gtatctgcac ctataaacac aataggtttt gcaacacgtg
     4381 tcatttctat cgaatcttta gcaaaacatg aatgtatatc ggtaatttgc actgaatata
     4441 caccggactc ttttacaatt attgaggagg ctgaagatga ggtattccat gtgtacgttt
     4501 cccatgtatt atcaacagat atttctaccg aatctccatc acaaatagtt acagaaggaa
     4561 ctataaactt cacttgagga gaatcatata tttgtataag attgaattga actttagtag
     4621 aatctttgtg tattgttgta tctatagcct gcaaacttac agaatatact cccgattgta
     4681 aatatgtatg tttaggattc aaatcattag aagaattacc atcaccaaaa ttccacgtat
     4741 agatatctcc atctatactt ttgttagtaa attgtacaga tagtgaatcg caaccttgta
     4801 tagtatcaat tgtaaaataa gcaataggat atggtaattt tactaatgaa tcgctaattg
     4861 gaacactcca attaccttga aaatcgccta cctgcatatg aattacatgt ttacctttgg
     4921 gt
//
LOCUS       DAOTOW010000069         4836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14463_length_4836_cov_3.094959, whole genome shotgun sequence.
ACCESSION   DAOTOW010000069 DAOTOW010000000
VERSION     DAOTOW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14463_length_4836_cov_3.094959"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="P5243_01855"
     CDS             complement(<1..182)
                     /locus_tag="P5243_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRS18221.1"
                     /translation="MRKLGLFAVLVMLTIAGVAQKKMTRGEYIEAYAPIAVKEMQRTG
                     IPASITLAQACLESNNG"
     gene            complement(360..4823)
                     /gene="dnaE"
                     /locus_tag="P5243_01860"
     CDS             complement(360..4823)
                     /gene="dnaE"
                     /locus_tag="P5243_01860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRS18222.1"
                     /translation="MFIIFDTETTGKAKNFNAPIHDIDNWPRMVQLAWQVHDERGEFI
                     VNKNYIIKPEGFEIPYDAQKIHGISTERALAVGHDIQKVLDEFAQDVHACKYVVGHNV
                     DFDISVVGCELVRLQKPNVFESLTSIDTMKNSIEYCKLPGGKGGSFKFPKLTELYEIL
                     FHESFVEAHNASADVNATARCFFEMIRLQIITNKHINDDECIKKFIQHNPTVCKLAKI
                     DLQSNFTPLENSSTESSSNKIKAEIVGDKTFVHLHVHSQYSILDGAASIQSLVKKAKK
                     DGMTALALTDHGNMHGIKEFHAACKKEGIKPILGCEAYIVQDLSIEKDKSNHHIILLA
                     KNLQGYKNLLKLISIANLKGLYYRPRIDKNLLQTYKEGLIVSSACLGGEVCRTLMEDG
                     VEAAEKVVLWYKEVFGDDFYLEMMRHPAQDENLRKEIYESQQYVNAELIKLARKHNIS
                     LIATNDVHFTNQDEATAHDLLICLNTGADVNDPKRMRYTREEWFKTTDEMYALWSDMP
                     EVLQNTSKLAEKIEQYTLDSAPIMPEFPIPVEFGTIETYKQKYTDEMLQKEFERYKDL
                     GGYDSVLRIKFESDYLEFLTKQGAVKRYGDNPDESTLERIDFELNTIKQMGFPGYFLI
                     VQDFIAQARKMGVLVGPGRGSAAGAVVSYCLGITNIDPIRYDLLFERFLNPDRISMPD
                     IDIDFDDDGRQRVLDWVVEKYGHDRVAHICTFGTMAAKSALKDVARVLKLPLSEANRI
                     TKEFPDNGKLDGAYAKVLELEKETGSLQKAIETIQSNKKKAIQEDKEKEILACDVQLF
                     IAQELVNAGKENNQIKIDTIANACVLEGSVRQAGVHACGILIGKNPLDEHIPLMPTKD
                     EVMLSTQYDGRFVEAIGLLKMDFLGLRTLTIIKEAIANIKLSTGIDLDIDAIDFADKK
                     TFELFGKGETTAIFQFESPGMKKHLRALKPNRFEDLVAMNALYRPGPMEYIPDYIARK
                     NGVKKVEYDHPLMEKYLKDTYGITVFQEQVMLLSRELANFTRGDSDVLRKAMGKKQID
                     VMNKLKLQFKEGCLANPAFIEGCKQNSKNPEELIEKIWKDWEAFAQYAFNKSHSVCYA
                     YIAYQTGFLKAHYPAEFMAAALSSNLSKADEISKLMEECSRMGLQVLVPDVNESQNNF
                     AVNKKGQIRFGLGAIKGVGEAASQTIISERSKGLFADIFDFVSRMNLRTVNKKNLESL
                     ALSGAFDGFGIERHRFFSENENEPSFIEILLKYGNASQTSTEPNMMSLFGDDEIAVEK
                     PAIPNPPRWSNMEYLRKEKEHIGIYLSAHPLDEYKMIISNFCRATLSQLDDMSKFFDK
                     EFTVAGMVTKVNILETKTGKQYTKFTLDDFNGSFEFTLFGKDQTEFSPFIVMGEKLAL
                     TLKVERNKYKENSEYEYKIKKIDPLDSLKDKIKQITIQININNINEHIIQEIKEIAEV
                     DETKGLPLTFMVYDRVENITLTLKSLNMRVELNADFIDYLVNHENLDYKLN"
BASE COUNT         1485 a          939 c          647 g         1765 t
ORIGIN      
        1 ccattatttg attctaaaca tgcttgagct aaggtaatac ttgcgggaat acccgttctt
       61 tgcatttcct ttactgctat aggcgcataa gcttctatat actcaccgcg agtcatcttt
      121 ttttgagcaa ctccagctat agtaagcatt actaataccg caaataaacc taattttctc
      181 atttatatac cttaattgca caaaaatatt gagtaaaaca atacagcact ataaaaaaat
      241 taaaattcta ttcacaagtt atgaaacatt acaaattctg taatatttac tacaaccata
      301 catgtatgat ttattctagc aaaatagtta tatatatgaa ttttagaatg ttatgaattt
      361 taattcaatt tataatctaa attctcatga tttactaaat agtctataaa atctgcattt
      421 aattcaacac gcatattaag cgattttaag gtaagtgtaa tattttcaac tctatcatat
      481 accataaacg ttaatggtaa accttttgtt tcatctactt ctgctatttc ttttatttcc
      541 tgaattatat gctcattaat attattaata tttatttgta tggtaatctg ttttatttta
      601 tcttttaaac tatctaaagg gtcaattttt ttaattttat attcatattc tgaattttcc
      661 ttatacttat ttcgttcaac ttttaatgtg agtgcaagct tctcgcccat aactataaaa
      721 ggcgaaaatt ctgtttgatc tttaccgaat aaagtaaact caaaacttcc attaaagtca
      781 tctaatgtaa atttagtata ttgttttcct gttttggttt ctaatatatt tactttggta
      841 accatgcctg caacagtgaa ttctttatca aaaaacttac tcatgtcatc taattgacta
      901 agagtagcac gacaaaaatt tgatataatc attttgtatt catcaagagg atgagcggat
      961 aaatatattc ctatatgctc tttttcttta cgtaaatatt ccatatttga ccaacgagga
     1021 ggatttggta tagcaggttt ttcaactgct atttcatcat caccaaacag cgacatcata
     1081 ttaggttccg tactagtttg cgaagcattt ccatacttta aaagaatttc tataaacgaa
     1141 ggttcattct cgttttctga aaagaatcta tgacgctcaa ttccaaatcc atcaaatgca
     1201 ccacttaatg ctaaactttc aagatttttt ttatttactg ttctcaaatt cattcgcgaa
     1261 acaaaatcaa atatatcagc aaataaccct tttgaacgct cacttataat tgtttgcgat
     1321 gcggcttcgc ccacaccttt tatagctcct aagccaaaac gtatttgtcc ttttttattt
     1381 accgcaaaat tattctgact ttcgtttacg tcgggcacca atacctgtag ccccattcta
     1441 ctgcattctt ccataagttt tgaaatctca tcggcttttg ataaattact gcttaatgca
     1501 gcagccataa attcagcagg atagtgagcc ttgagaaaac cggtttgata tgctatataa
     1561 gcataacaca ccgaatgtga cttattgaat gcgtattgag caaaagcttc ccaatccttc
     1621 caaatttttt ctattaattc ttccggattt ttagaattct gcttacaacc ttcaataaaa
     1681 gcaggatttg ccaaacaccc ttctttaaat tgaagtttta atttattcat tacatctatt
     1741 tgttttttac ccatagcttt acgaagcaca tcagaatctc cacgtgtaaa atttgcaagt
     1801 tcgcgcgaca aaagcattac ttgttcttgg aaaaccgtaa taccataggt atctttcaaa
     1861 tacttttcca taagcggatg atcatattcc acctttttaa ctccattttt acgggcaata
     1921 taatcgggaa tatattccat aggtcccggt ctgtataatg cattcattgc aactaaatct
     1981 tcgaaccggt taggcttcaa agcacgtaga tgttttttca ttccgggaga ctcaaattgg
     2041 aatatagcag tagtttcacc tttaccaaaa agttcaaatg tttttttatc ggcaaaatct
     2101 attgcatcta tatctaaatc aattccagta gaaagtttaa tatttgcaat tgcttctttt
     2161 ataatagtta aggttctcaa ccctagaaag tccattttga gcaatcctat agcttctaca
     2221 aatctaccat catattgtgt agaaagcatt acttcatctt tagttggcat taatggaata
     2281 tgctcatcta acggattttt acctattaaa attccgcaag catgtacacc tgcttgtctt
     2341 acagacccct caagcacaca cgcattggca attgtatcaa ttttaatttg attgttttct
     2401 tttcctgcat tcacaagttc ttgagctata aataattgca catcacacgc aagaatttct
     2461 ttttccttat cttcttgaat agcttttttc ttgtttgatt gaatagtttc tatggctttt
     2521 tgtaaactac ctgtttcttt ctccagttcc aacaccttag cataagcacc atcaagtttt
     2581 ccattatcgg gaaattcctt agtaatccta ttagcctcac tcagtggtag tttcaatact
     2641 cgtgcaacat ctttcaaagc actttttgcg gccatggtac caaaggtaca aatatgagct
     2701 actctatcat gcccatattt ttccacaacc caatccagaa cccgttgtct cccatcgtcg
     2761 tcaaaatcaa tatcaatatc gggcatcgaa atacgatcgg gattaagaaa tcgttcaaat
     2821 agtaaatcat aacgaattgg atctatatta gttataccca aacaatatga aaccacagca
     2881 cctgctgcag atcctcgtcc cggacctacc aatactccca ttttgcgagc ttgtgctata
     2941 aaatcttgca ctataagaaa atacccggga aaacccattt gctttattgt attcaattca
     3001 aaatcaattc tctccaatgt tgattcatcg ggattgtcac catatctttt tactgcaccc
     3061 tgttttgtta aaaattctaa ataatccgat tcaaatttta ttcgcaatac cgaatcatat
     3121 ccacctaaat ctttataccg ttcaaattct ttttgcaaca tttcatcggt atacttttgt
     3181 ttatatgttt ctattgtgcc aaattcaaca ggtataggaa attcaggcat aattggcgcg
     3241 gaatctaaag tgtattgctc tattttttca gccaattttg acgtattttg caatacttcg
     3301 ggcatatcac tccacaatgc atacatttca tcggtagttt taaaccattc ttcacgagta
     3361 tatcgcattc gtttggggtc atttacatcg gcccctgtgt ttagacatat aagcaaatca
     3421 tgagctgttg cctcatcttg attggtaaaa tgaacatcat tggttgcaat aagagaaata
     3481 ttatgcttgc gagctaactt aatcaattct gcattcacat actgttggct ttcgtaaatt
     3541 tcttttcgta aattttcatc ttgtgcagga tgacgcatca tttctaaata aaaatcatct
     3601 ccaaaaactt ctttatacca caacacaact ttttcggcag cctctactcc atcttccata
     3661 agtgttctac acacttcgcc tcccaaacat gcagaagata ctatgagccc ttctttatat
     3721 gtctgcaata aatttttatc aatccttgga cgataatata agcctttaag atttgctatt
     3781 gatataagct tcaataaatt tttatatcct tgtaaatttt ttgctaacaa aattatatga
     3841 tgatttgatt tatctttttc tatagataaa tcttgtacaa tataagcttc acagcctaat
     3901 ataggtttta taccttcctt tttacatgct gcatgaaatt cctttattcc gtgcatattt
     3961 ccatgatcag taagagctag ggctgtcatt ccgtcttttt ttgctttttt tacaaggctt
     4021 tgtatagagg ctgctccatc aagtatagaa tattgcgaat gcacatgtaa atgaacaaat
     4081 gttttgtctc caactatttc tgctttaatt ttgttagaag agctttctgt tgaagaattt
     4141 tcaagaggag taaaatttga ttgtaaatct attttagcta atttacaaac agtaggatta
     4201 tgttggataa actttttaat gcactcatca tcattaatgt gcttattggt aattatttgt
     4261 aatcgaatca tttcaaaaaa gcaacgagct gtagcattta catcagccga agcattatga
     4321 gcctctacaa atgattcatg aaataatatt tcatataatt cggtgagttt tggaaattta
     4381 aaacttccac ctttaccacc cggcaactta caatattcta tagaattttt catggtatca
     4441 atgcttgtaa gcgattcaaa aacattaggt ttttgtaatc gaacaagctc acagccaact
     4501 actgaaatat cgaaatcaac attatgccct actacatatt tacaggcgtg tacgtcttgt
     4561 gcaaattcat caagtacttt ttgtatatca tgcccaactg ctaaagctct ttctgttgaa
     4621 atcccatgta ttttttgtgc atcgtagggt atttcaaacc cttcaggttt tattatataa
     4681 ttcttattta caatgaattc tcctctttca tcatgaactt gccaagccaa ttgaaccatt
     4741 cttggccaat tatcgatatc atgaatagga gcattaaaat ttttagcttt accggtagtt
     4801 tctgtatcaa atattataaa catattgtat ttttta
//
LOCUS       DAOTOW010000070         4827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14494_length_4827_cov_3.530176, whole genome shotgun sequence.
ACCESSION   DAOTOW010000070 DAOTOW010000000
VERSION     DAOTOW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14494_length_4827_cov_3.530176"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="P5243_01865"
     CDS             complement(<1..>1040)
                     /locus_tag="P5243_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRS18223.1"
                     /translation="TSLHKGFIDNTLKICCFTDHEIFERYHAHSVRKKIISAESFSIQ
                     ELKNLHPGDFIVHIDHGIGKFAGLERMTVNGKIQETIKLIYKDNDILYVNIHNLHKIS
                     KYKGKEGSQPNLHKLGSPVWQTTKQKTKNKVKDIAKELIALYAERKAKEGFRFSPDTY
                     MQQELEASFFFEDTPDQITATQKVKQAMELPYPMDMLVCGDVGFGKTEIAIRAAFKAV
                     ADNKQVAVLVPTTILALQHFKTFTARLADFPCTVDYICRLRSTKQQKETLQKVKQGNV
                     DILIGTHRLIGKDVLFKDLGLLIIDEEQKFGVGMKEKLKQMKVNVDTLTLTATPIPRT
                     LQFSLMGARDLA"
     assembly_gap    1041..1050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1051..2184)
                     /locus_tag="P5243_01870"
     CDS             complement(<1051..2184)
                     /locus_tag="P5243_01870"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18224.1"
                     /translation="MNISEFSKLFAQHHQVQNLITQLQQHISHKAVLQKLCGSMQWFI
                     GAHIFSHLSTHMWIIMPNNDMASYVHSDLASIIGAKHLLFIPPSHKRSVLYNQPDRSH
                     MLLRTEALEKLVSSDTRYIIITSPESLIEQVISKDSFQQKIQWLHVGENVDTKFINEL
                     LYEFGFERVDFVVEPGQFSVRGSIIDIFSYSNDAPFRIDFFGNEVESIRTFNIDDQRS
                     IEQLESCAIVPDVQNQTEQNIYIPLYEYFTNSIVVSYDLSLCIGNLQSLYEQCVSKIH
                     NKQLPISENSLLSHNEFTVFLESIRVCEIASNPYFPNATIYEFDTIPQPAIHKNFELL
                     GKLFVEYQSLDYTNYFITHTISQYNRIHDIFKEINPHVRLTPII"
     gene            complement(2191..2715)
                     /locus_tag="P5243_01875"
     CDS             complement(2191..2715)
                     /locus_tag="P5243_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18225.1"
                     /translation="METLRIILLIILPSALVFATAYFSIKQFFTNEQKKRESENLIQN
                     QKITVPIRLQAYERLILLLERINPQSLLLRIQNSNSTAGQLHNDLLIAVRAEFEHNLS
                     QQIYISSQSWLAVKRAKEVLIKIINEEASKIAVNAPASVLSEAILMHIVSLSDNPIQI
                     AIDTLKVEVTRFLS"
     gene            2855..4390
                     /locus_tag="P5243_01880"
     CDS             2855..4390
                     /locus_tag="P5243_01880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRS18226.1"
                     /translation="MSDDIFKKIIAHSKEYGFIFPSSELYDGLGAVYDYGQLGAELKN
                     NIKQYWWNAMVRLNENIVGIDSAIFMHPTIWKASGHVDAFNDPLIDNKDSKKRYRADV
                     LIEEHREKMNQKIEKELEKGRKKFGDSFDEAQARATNPNIQREQAKIDEITARFVKAL
                     ETSNLEELRQIIIDSGIVCPISGTANWSDVRQFNLMFETKLGATAEGASTIYLRPETA
                     QGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPGT
                     EMEWFNIWKQTRLAWHKALGFGDENYRYHDHEKLAHYANAACDIEFNFPMGFKELEGI
                     HSRTDFDLSQHQQYSGKKITYFDPELNESYVPYVVETSVGLDRTFLAILSKSYSEEQV
                     ENETRVVLRIPSALAPIKAAVMPLVKKDGLPEVAREIIKTLSLEFNCQYDEKDSIGKR
                     YRRQDAIGTPFCITIDHQTLQDNMVTIRDRDTMQQERVPIDSLVQIVDAKVSMNNLLR
                     QLM"
     gene            4402..>4827
                     /locus_tag="P5243_01885"
     CDS             4402..>4827
                     /locus_tag="P5243_01885"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRS18227.1"
                     /translation="MPVTKRQNKLIQQRIISSYSSTLISVALVLFILSLVGLIVLNEH
                     KISNHIKEKVGFTIFLKQDVKEADLQGLRKRLDAMEMVKSTEYVSAEEASKRLAVTLG
                     KDYIDLAQGNPVPPSIEVRLFAEYATAENFVKIEQQMQGN"
BASE COUNT         1608 a          722 c          864 g         1623 t
ORIGIN      
        1 tagctaagtc gcgagcgccc ataagagaaa attgaagagt gcggggtatt ggagtagctg
       61 taagagttag agtgtcaaca tttactttca tttgttttag tttttctttc attccaactc
      121 caaatttttg ctcttcgtca ataattaata agcccaaatc tttaaataat acatctttgc
      181 ctatgagtct atgagttcct ataagaatat ctacattgcc ttgttttact ttttgcaatg
      241 tttctttttg ttgttttgtg ctgcgaagtc tgcatatata atcaacagta cacgggaagt
      301 ctgctaaacg agctgtaaat gttttgaagt gttgtaatgc taaaatagtt gtaggaacca
      361 atacagcaac ttgtttattg tcggcaactg ctttaaaagc tgcacgtata gcaatttctg
      421 tttttccaaa acctacatcg ccacatacta gcatatccat aggataggga agttccattg
      481 cttgttttac cttttgtgtt gctgtaattt ggtcgggagt gtcttcaaag aaaaatgatg
      541 cttctaattc ctgttgcata taggtatcgg gcgaaaatcg aaatccttct tttgctttac
      601 gttcagcata taaagcaatt aattctttag caatatcttt tactttgttt ttagtttttt
      661 gtttggttgt ttgccatacc ggagaaccca atttatgtaa attgggttga gaaccttctt
      721 tgcctttgta ttttgaaatt ttatgtaaat tatgaatatt tacatatagt atatcattgt
      781 ctttgtatat taattttatt gtttcttgta tttttccgtt tacggtcatt cgttccaatc
      841 ctgcaaattt tcctatacca tggtctatat gaacaatgaa atcgccggga tgtaaattct
      901 ttagttcttg aattgaaaaa ctttcggcag aaattatttt tttgcgaaca gaatgcgcat
      961 gataccgttc aaaaatttca tggtctgtaa aacaacagat tttaagtgta ttatcaataa
     1021 aacctttgtg tagcgatgtt nnnnnnnnnn tataatagga gtaagacgaa catgaggatt
     1081 tatttcttta aaaatatcat gtatgcgatt gtattgtgaa atagtatgag taataaaata
     1141 atttgtataa tctaaacttt ggtattcaac aaacagttta cctaacagtt caaaattttt
     1201 atgtatagca ggttgaggaa ttgtatcaaa ttcatatata gttgcatttg gaaaatatgg
     1261 gtttgaagct atttcacata ctcgtataga ttccaaaaaa acagtgaact cattatgtga
     1321 taataatgaa ttttcagata ttggtaattg tttgttatga attttagata cacattgttc
     1381 gtagagtgat tgtaaattgc caatgcaaag agataaatca tagcttacca caatggaatt
     1441 tgtaaagtat tcatatagtg gaatatatat gttttgctcg gtttgatttt gaacatcggg
     1501 tactatagca catgattcta attgttctat agaccgctga tcatcaatat taaatgtacg
     1561 aatactttca acctcattgc caaaaaaatc tattctaaac ggagcatcgt tagaataact
     1621 aaaaatgtcg ataatactgc cccgcaccga aaattgtccg ggttctacaa caaagtctac
     1681 ccgttcaaat ccaaactcat ataataattc atttataaat ttggtatcta cgttttctcc
     1741 aacatggagc cattggattt tttgttgaaa tgaatcttta gaaatgactt gttctattaa
     1801 tgattcgggt gaggttataa taatataacg cgtatctgat gaaactaatt tttctaatgc
     1861 ttccgtacgt aaaagcatgt gcgacctatc cggttgatta tacagtaccg aacgtttatg
     1921 tgatggggga atgaaaagta gatgttttgc tccaataatg cttgctaaat cactatgtac
     1981 atatgatgcc atatcattgt ttggcattat aatccacata tgagttgaaa gatgcgaaaa
     2041 aatgtgtgct ccaataaacc actgcataga accacacaat ttttgtaaaa cagctttgtg
     2101 agaaatatgt tgttgtaatt gtgttataag gttttgaacc tgatggtgtt gtgcaaaaag
     2161 ttttgaaaat tctgaaatat tcactcctac ttacgataaa aaacgtgtga cttctacttt
     2221 caacgtatct attgcaattt gaataggatt gtcagaaagt gatactatat gcataagaat
     2281 agcttcactg agaacagatg ccggagcatt taccgcaatt tttgaagctt cttcgtttat
     2341 aatttttatt aatacttctt tggcacgttt tactgccaac catgattgag aacttatata
     2401 aatttgttga gacagattat gctcaaattc tgcacgaact gcaataagta aatcattatg
     2461 aagttgacca gctgtagagt ttgaattttg aattcgtaat aataatgatt gggggttgat
     2521 acgttcaagc agtaaaatga gtcgttcata tgcttgtaat cgtataggaa cagtaatttt
     2581 ttgattttgt ataagatttt cggattctcg ttttttttgt tcattagtaa aaaactgttt
     2641 aatgctaaaa tatgccgttg caaacacgag tgccgaaggt aatattataa gtaatatgat
     2701 acgtaaggtt tccatacgat taaatttaat atacaaaaat agaaaaaaac atatgatgta
     2761 acacaactaa gatgtatgtt ttttgaattt tacaaatgta atatctatat ttgcagcaaa
     2821 ttttatatac gttatactat tatcattcaa aaacatgtca gacgatattt tcaaaaaaat
     2881 tattgcccat tctaaagaat acggatttat tttcccttcg agcgaattat atgatggatt
     2941 aggagctgtg tatgattatg gtcaattggg tgcagaactt aaaaataaca taaaacaata
     3001 ttggtggaat gctatggttc gcctcaacga aaatattgta ggtatagatt cagctatatt
     3061 catgcatcct actatttgga aagcatcggg acatgttgat gcttttaatg accctttgat
     3121 agacaacaaa gattctaaaa agcgatatag agccgatgtg ttaattgaag aacatcgtga
     3181 aaaaatgaat caaaaaattg aaaaagaact tgaaaaaggt cgtaaaaaat ttggagattc
     3241 atttgacgaa gctcaagcgc gtgcaacaaa cccaaatatt caaagagaac aagcaaaaat
     3301 tgatgaaatt actgcacgtt ttgtaaaagc acttgaaact tcaaaccttg aagagttacg
     3361 tcaaattata attgatagtg gtattgtgtg tcctatttct ggtaccgcaa attggtctga
     3421 tgttcgtcag ttcaatttaa tgtttgaaac caaattgggt gctactgccg aaggtgccag
     3481 cacaatatac ttacgtcccg aaacagcaca aggtatattt gtaaattatt tgaacgttca
     3541 aaaaacagga cgaatgaaaa taccgtttgg tatagctcaa attggtaaag catttcgtaa
     3601 cgaaatagta gcacgacaat ttattttccg tatgcgggag tttgaacaaa tggaaatgca
     3661 gttttttgtt cgtcccggta ccgaaatgga gtggttcaat atatggaaac aaacccgttt
     3721 agcatggcac aaagcattag gtttcggcga tgaaaattat cgttatcacg atcacgaaaa
     3781 acttgcacat tatgcaaatg cagcatgtga tattgaattc aattttccaa tgggtttcaa
     3841 agaattagaa ggaattcatt cacgtaccga ttttgattta agtcaacatc aacaatattc
     3901 aggtaaaaaa atcacttatt ttgaccctga attaaatgaa agttatgtgc cgtatgttgt
     3961 agaaacatcg gttggtttgg accgtacatt cttggcaatt ttatcaaaat catatagcga
     4021 agaacaagtt gaaaacgaaa ctcgtgttgt attgcgtata ccttcggctc ttgctcctat
     4081 aaaagcagct gtaatgcctt tggtaaaaaa agacggatta cccgaagttg ctcgtgaaat
     4141 tataaaaaca ttgtcgttgg aatttaactg tcaatacgat gaaaaagatt cgattggaaa
     4201 acgttaccgc cggcaagatg ctataggtac accattctgt ataactatag accaccaaac
     4261 attacaagat aatatggtaa ctattcgcga tcgtgataca atgcagcaag aacgagttcc
     4321 aattgattcg ttagttcaaa tagttgatgc taaagtttca atgaataatt tattgcgtca
     4381 acttatgtaa atgttttgat tatgcctgtt acgaaacgtc aaaataaatt aatacaacaa
     4441 cgaataattt cgtcgtattc aagtacacta attagtgtag cgttagtatt atttatattg
     4501 agtttggtgg ggctaatagt attaaatgaa cataaaatat caaaccatat taaagaaaaa
     4561 gttggcttta ctatattttt aaaacaagat gtaaaagaag ccgatttaca aggattacgt
     4621 aaacggctcg atgctatgga aatggtaaaa tctaccgaat atgtgtctgc cgaagaggca
     4681 agtaaacgct tggcagttac acttggaaaa gattatatag atttagctca aggtaatcct
     4741 gttcctcctt ctatagaagt gcgtttgttt gcagagtacg caaccgccga aaattttgta
     4801 aaaattgaac aacaaatgca gggtaat
//
LOCUS       DAOTOW010000071         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14560_length_4810_cov_3.299895, whole genome shotgun sequence.
ACCESSION   DAOTOW010000071 DAOTOW010000000
VERSION     DAOTOW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14560_length_4810_cov_3.299895"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="P5243_01890"
     CDS             <1..405
                     /locus_tag="P5243_01890"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRS18228.1"
                     /translation="IRYRTSQMPIKEMTYLFLVIGLSVVNALANKKISYAELLFTNIA
                     ILGITYILEKLWLLRHESHKIIRYDNIDLIKPEKYDELKADLEKRTGLTINRIDVGDI
                     NFLSDSARIQIYYYEKDNDINQLAENTKGGDD"
     gene            467..1078
                     /locus_tag="P5243_01895"
     CDS             467..1078
                     /locus_tag="P5243_01895"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRS18229.1"
                     /translation="MYSQTHTNELWTELGVSSEIVYNLQAEAIIETRFQEVGSYIKSI
                     SGEVGVSYKLIKPISVGITYKCTEKNRPQGFFAAHTGAFTFSYRQKYGNVRVAYRNKF
                     EINRDTYINEYTDLYPQFEDRNRFKLSYARKKAFISPSLSIETFHTGYNQSQFLLSEI
                     RYSCGVDFNLKNKFEASTAYTYKQSFGKSVNTSIYSISVSKSF"
     gene            1112..>4810
                     /locus_tag="P5243_01900"
     CDS             1112..>4810
                     /locus_tag="P5243_01900"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS18230.1"
                     /translation="MKYFLCILFIISLFVRVTCFAQELIINEIVANNTSGYTNAAGKT
                     PDWIELKNVSTKTINLSQYSLADSKTKSHPWQLPNVQLAPGAFYVIEATNPKELVVDW
                     ETIIDKGAVWKYIVPTTELPTQWKQLGFNDASWSQGPSGFGYGDNDDATVLENCISVF
                     IRKTFSIADVSIISSAILHMDYDDGFVAYLNGVEIARASIAGNPPSYNVGASGHEANW
                     YQKLPIESFQIADITDIIQNGENVLCIQIHNTDINSSDLTAIPIFTLGYSEKKIGNTG
                     GSTYIQLPQAGNQAPFKVSASKETIYLFKNTILADSIAFEDLPQDVSIGRARNETKST
                     VYFTSPTFGKENSVIVYTPKTLSKPVVSVAGGVYSKNQVITIYSPDNNTQIYYTTDGS
                     IPTENSKLYTAPISVSASTVLKYRAYKSGYVPSDVVTHSYIIFKRTHRLPIVSITYNH
                     NDFFDPKTGIYVLGPNAQSDPPNFGANFWQDWERPCHVEVFNPDGSTAFSHNLGVKIA
                     GNWSRANPQKSLKFYARDKYGDEAIEYQIFKDKPIYSFQSFILRNSGNDYCNTHMRDG
                     AIQSLCRNMGIDYQAYQPAVVYMNGQYWGILNFREKINKDYIAENHGIPLDSFAILAN
                     INEPVYGDIQNFRDLYAFISNNDISIPANYERVKQDLDIDNYIKYNVIEMFAVNEDWP
                     GNNVKAWREYGSQGKWRYILYDLDFGFGIWDANKVYRNMLTFALTNDESIGWPNPPWS
                     TLMLRKLTQNQEFNALFLNHVADRLNTTFHPDSISRHIDSLKNLIINELSYHATRWNA
                     NSDDMYANILGMKEFGKNRGAIMRQHFEEYFNTGGSYPLTITSSHSNPGRIHVNTIDI
                     TKFPWSGLYFNNNTITLTAIPAPGYTFVTWQGAVVSTNPTITLTKNSATSVHAVFTYN
                     AQNIPQIVISEINYNSNSESDTGDWIELYNKSNTDQDVSGWTITTQSPNKPFIFPQGS
                     VIPANSYCIVSSSLSKYNTMYPLATYMRFGNIPFTLSKSQETLFLRNAQGYTIHSISY
                     NDTYPYPKKCDGYGYTLECTDANNFVQNPMIWRAPTYMGTPGKTNTPLIRNVNYNAIQ
                     INEVMYNPHDNAKCGDWIELYNSGNSLVDISGWVLRDGNNSISIIPKPTAIAAKSYIV
                     FCENPQKFATVYPQVTCTPLNIGFKSSGDFIRLADQYELVVDSVQYSVFSDLGITTNG
                     TGRTIIRDANNIWTYGNVGGTPEANNIKG"
BASE COUNT         1715 a          700 c          822 g         1573 t
ORIGIN      
        1 attcgttata ggacaagtca aatgccaatt aaagaaatga cgtatttatt tttagttatt
       61 ggtttgtcgg ttgtaaatgc attggctaat aaaaaaataa gttatgcaga gcttttattt
      121 acaaatattg cgattcttgg aattacctat attttagaga aattatggtt gcttagacat
      181 gagtcgcaca aaataattcg atatgataat attgatttaa taaaacctga aaaatatgat
      241 gaattaaagg cagatttaga gaaacgtaca ggtcttacaa ttaatagaat tgatgttggc
      301 gatattaatt ttttaagtga ttcggcacga attcaaattt attattatga aaaagataat
      361 gatataaatc aattggcaga aaacacaaaa ggtggagatg attaagtagg tgagaaaaaa
      421 ctatttcata ggattatttt ttttaattgt atgtggttat agtactttgt atagtcaaac
      481 tcatacaaat gaattgtgga cggaattagg agtttcatct gagatagttt ataatttaca
      541 agcagaagct ataatagaaa cacgatttca agaagttgga tcatatatta aatcaatttc
      601 gggtgaagta ggagtttcat ataaacttat aaaacctatt tctgttggaa ttacatataa
      661 gtgtaccgaa aaaaatagac cacagggttt ttttgcagct cataccggag cttttacatt
      721 ttcatatcgt caaaaatatg gtaatgttcg tgttgcatat cgaaataaat ttgaaataaa
      781 tcgcgataca tatatcaacg aatatacaga tttgtatcca caatttgaag acagaaaccg
      841 atttaaatta tcatatgctc gtaaaaaagc gtttatttca ccttctcttt caatagaaac
      901 atttcatact ggatataatc aaagtcaatt tttattaagt gaaatacgat attcatgtgg
      961 ggtagatttt aatttaaaaa ataaatttga ggcaagtaca gcatatacat ataaacaaag
     1021 tttcggaaaa tccgtaaata catctatata ttctataagt gtttcaaaat ctttttgata
     1081 tactaaaaaa atatatataa taaatataac aatgaaatat tttttatgca tattatttat
     1141 aataagttta tttgtaaggg ttacttgctt tgctcaggaa ttaattataa atgaaattgt
     1201 tgcaaataat actagtgggt ataccaatgc tgccggtaaa acacccgatt ggatagaatt
     1261 aaaaaatgtt tctactaaaa ctataaatct gtctcaatat agtttagccg attcaaaaac
     1321 aaagtcacat ccatggcaat taccaaatgt acaacttgct ccaggagcat tttatgtgat
     1381 agaagctact aatcctaaag aattggttgt tgattgggaa acaattatag ataaaggtgc
     1441 tgtgtggaaa tatatagttc ctactaccga attgccaaca caatggaaac aattaggctt
     1501 caatgacgct tcatggtctc aaggtccttc agggtttgga tatggagata atgatgatgc
     1561 aacggtttta gaaaattgta tttcggtatt tattcgtaaa acattttcta ttgcagatgt
     1621 ttcaataatt tcgtcggcaa tactgcacat ggattacgat gatgggtttg ttgcatatct
     1681 taatggagtt gaaatagctc gtgcttctat agcaggaaat cctccttcat ataacgtggg
     1741 agcaagtggt cacgaggcta attggtatca gaaattacct atagaatcat ttcaaattgc
     1801 agatattaca gacataattc aaaatggaga aaatgtattg tgtattcaaa tacataatac
     1861 agatataaat tcatcggatt tgacagcaat accaatattt acacttggat attctgaaaa
     1921 aaaaattggt aataccgggg gttcaacata tattcagttg cctcaagcag gtaaccaggc
     1981 tccttttaaa gtaagtgctt ccaaagaaac aatatatctt tttaaaaata ctatacttgc
     2041 tgatagcata gcatttgaag atttaccgca agacgtttct attggtcgtg cccgaaatga
     2101 aactaaaagt acggtatatt tcacaagtcc tacatttggg aaagaaaatt cggttattgt
     2161 ttatacacca aaaacattgt caaaacctgt tgtttcagtt gcaggagggg tgtattccaa
     2221 aaatcaagtg attactatat atagtcccga taataataca caaatttatt atacaaccga
     2281 tggaagtatt ccaacagaaa attctaagct ctataccgct cctatatcag tttctgccag
     2341 cacagtgtta aagtatagag cctataaatc tggttatgta ccaagtgacg tagtaacaca
     2401 ttcgtatatt atcttcaaac gaacacacag attaccaata gtatcaataa catacaatca
     2461 taatgatttt tttgacccta aaaccggcat ttatgtgtta ggtcccaatg cgcaatcaga
     2521 tcctccaaat tttggagcaa atttctggca agattgggaa cgcccatgtc atgttgaggt
     2581 ttttaatcct gatggttcaa cagctttttc tcataattta ggtgttaaaa ttgcaggaaa
     2641 ttggagtaga gcaaatcctc agaagtcgtt aaaattttat gcacgcgaca aatatggcga
     2701 cgaagcaata gaatatcaaa tttttaaaga taagcctatc tattcgtttc agtcatttat
     2761 tcttcgaaat tcaggaaatg attattgtaa tactcatatg cgtgatggag ccattcaatc
     2821 tttgtgtaga aatatgggga ttgattatca ggcatatcaa cctgcggtag tctacatgaa
     2881 tggacaatat tgggggatat taaattttcg tgaaaaaatt aataaagatt atatcgctga
     2941 aaatcatggt attccacttg atagttttgc tatattggca aatataaatg aaccggtata
     3001 tggtgatatt caaaatttta gagatttata tgcttttata agcaacaatg atatttcaat
     3061 acctgctaat tatgaaaggg taaaacaaga tttagatatt gataattata ttaagtataa
     3121 tgtaattgaa atgtttgccg taaatgaaga ttggcccggt aataatgtaa aagcatggag
     3181 agagtatggt tcacaaggca aatggcgata tattttatat gatttagatt ttggttttgg
     3241 tatttgggat gctaataaag tatatagaaa tatgcttact tttgccttaa ccaatgatga
     3301 atctattggg tggccaaatc caccatggag cacgcttatg ttacgaaaat taacgcaaaa
     3361 tcaagagttt aatgcattat ttcttaatca tgttgcagat agactaaata caacatttca
     3421 tcctgattct atatcgcggc atatagattc attaaaaaat ttgataataa atgaattatc
     3481 atatcatgct acccgatgga atgccaatag tgatgatatg tatgctaata tattgggcat
     3541 gaaagaattt ggaaagaata gaggtgctat aatgcgtcaa cattttgagg aatattttaa
     3601 taccggaggt agttatcctc ttactattac cagttcgcat agcaatcccg gacgaattca
     3661 tgtaaataca atagatatta ctaagttccc atggtcagga ttatatttta ataataatac
     3721 cataactctt actgccattc ctgctccagg atatacattt gttacatggc aaggtgcagt
     3781 agtgtcaaca aatcctacaa ttacacttac taaaaattct gctacttctg tacatgccgt
     3841 ttttacatac aatgctcaaa atataccaca aatagtaatt agtgaaatta attataattc
     3901 aaattcagag tctgatacag gagattggat tgaattgtat aataaatcaa atactgatca
     3961 agatgtgtct ggttggacaa ttacaacaca atcaccaaac aaacctttta tatttcctca
     4021 ggggtctgta attcctgcaa attcgtattg tattgtatca tcttctctat caaaatataa
     4081 tactatgtat cccttggcaa cgtatatgcg ttttgggaat ataccattta cattatctaa
     4141 atcgcaagaa acattatttt tacgaaacgc acaggggtat actatacatt ctatctcata
     4201 caatgataca tatccatatc ctaaaaaatg tgatggatac ggatatactt tagaatgtac
     4261 cgatgcaaat aattttgttc aaaatcctat gatttggcga gcacctacat atatggggac
     4321 accgggtaaa accaatacac ctttgattag aaatgtaaat tataatgcta ttcaaattaa
     4381 tgaagtaatg tataacccac acgataatgc aaaatgtgga gactggattg agttatataa
     4441 ttcaggtaat tcacttgtag atatatcagg ttgggtttta cgtgacggta ataattcaat
     4501 aagtattatt ccaaagccta ctgcaattgc tgcaaagtca tatatagttt tttgtgaaaa
     4561 tcctcaaaaa tttgcaacag tatatccgca agttacatgt actcctctca atattgggtt
     4621 caaaagcagt ggtgatttta ttcgcttagc agaccaatat gaattagttg tagattcagt
     4681 ccaatatagt gttttttctg atttgggtat tacaacaaat ggtacaggac gaactataat
     4741 acgtgatgca aataacatat ggacatatgg taacgtagga ggcactcctg aagcaaacaa
     4801 tataaagggt
//
LOCUS       DAOTOW010000072         4809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14565_length_4809_cov_3.194994, whole genome shotgun sequence.
ACCESSION   DAOTOW010000072 DAOTOW010000000
VERSION     DAOTOW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14565_length_4809_cov_3.194994"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="P5243_01905"
     CDS             complement(<1..2201)
                     /locus_tag="P5243_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18231.1"
                     /translation="MNRIKSFLVTVCIIAGTTLVFSQNTERLSKSNRIFNEAVSLFDN
                     QKFAAAQQKFIVISTSEDYAQTIRTDAEYYIAVCALRLFNGDAEMRIKQFIEKHPEST
                     RIYHAYFEYANYLFREQHYPEALIWYELVDIKKFNNTEISEYFYKIGYSYFKNNDYAR
                     AKPFFKEVKDSKSVYAQNALFYFSYLEYYDKNYASALKGFEFLADSTMFSTVVPPYIC
                     QIYYKQQQYDKVIQYARTIEGIVNPQQLPEVYRVLAGAYFKTLQYNLALPYYEKYIET
                     SLSPSKEDLYELGYLYYRDGKYAKAADLFSQVTSQNDTIAQNAYYHLGDCYLKLGIKD
                     KARNAFMAAMQYNVFPSIKEDATFIHAKLIYELSYAPFNEAITATQSFLAQYPSSKYK
                     DEANSLLVKIFMSSKNYKDALASLEQIKVLTTDLKIAYQQIAYYRGLELINNIDYTQA
                     IQMFDKSMQYGTFDRTIQAYSIYWKAECYYKLLQYDKARALFNEFVLVPGAFNSPEYK
                     RAHYSIAYTFFEQERFADAATWFRKYLDIADNKKDDFYSDAAIRTGDCYYMLKDFKQA
                     IRFYTIAVNIGKFDVEYALFQEAFCQGLAGNHEAKISSLAKFMKQYPSSMYYDDALFE
                     QAEAYMKADQESKASENYKVIVSNYKASSYIKKSLLQLAYIEYNKQKLDDALLMYQRV
                     LQQYPGSQESQTALNMIRNIYKKKNDIDGFSNYVKQIGGHANVTEAQLDSL"
     gene            2437..3120
                     /gene="deoC"
                     /locus_tag="P5243_01910"
     CDS             2437..3120
                     /gene="deoC"
                     /locus_tag="P5243_01910"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRS18232.1"
                     /translation="MNYSKYIDHSLLHPTATHNDIAKLCREAIEFDVAAVCIQPIYIQ
                     FAQTLLQHTPISVATVIGFPHGTNSIETKCFETKLAIKHGATEIDAVICIPQVIAQEW
                     KYIETEIHELSTICKQHNICLKIIFETTYLNEYHIIKLCEICSKEHVSFVKTSTGFDF
                     IKHESGLYTTKGAQISHIQLMKKHCFNNTQIKASGGIRSASYFIECIEAGATRIGTSA
                     TKDILKALP"
     gene            3164..4705
                     /locus_tag="P5243_01915"
     CDS             3164..4705
                     /locus_tag="P5243_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18233.1"
                     /translation="MVKLFTRHLITVISALLFTCVVYAQDEFMIHGRIQTIDNSIEGA
                     TVRVMHDYLTIKKIILDKTGAYTVYLPYGKNYSLLYEKKGYQTIQIDALLDLPKDVKQ
                     CCYRPLELSFHLTKPDSTHGDMFNKAFHTIQYDKNYKGFIYDIDIDYMIQQRIVNAQI
                     FKQTLLAAQEGNSSRNDSILVEKKYLALINQGTEYYNQKQFYAARKFFLEASKIKPER
                     MYPQYKLEDIKTELQRFETKAELLGINLDSLVAKELAAIEPKIEEKKPYPPYIPLTDA
                     QVEEIFKNDLKKQILKSSSNVAEANRTIALMNELFKDDMKKVTTADFKKQSNKDSNKP
                     EIDKREVGIKPKQTNKQVEVAKATTETKKVEEVVQKPKATEPNKEDQQEKIQDIADVI
                     TSKPEKKPITTKETTEKPVKKVVDYNTYQDSLRARYPDERTIEVTQDAHKKTTRVFMN
                     NGKIVEIYAMVEHAWGATYYFIEEYPSGAQSIGYAAFMSRTKLYDLQNNKPAEIKDSV
                     PAQKK"
BASE COUNT         1826 a          748 c          660 g         1575 t
ORIGIN      
        1 ctgagtgaat ccaattgcgc ttcagttaca tttgcatgtc caccaatttg ttttacataa
       61 tttgaaaacc catcaatatc atttttcttt ttataaatat ttcgaatcat atttaaagcc
      121 gtttgcgact cctgcgagcc cggatattgt tgcaaaacac gctgatacat aagtaacgca
      181 tcatctaatt tttgtttatt atattctata tatgccaatt gcaacaatga ttttttgata
      241 tatgaacttg ctttataatt agatactatt actttataat tttcagaagc ttttgattcc
      301 tgatctgctt tcatataggc ttcagcttgt tcaaatagag catcatcata atacatagaa
      361 cttggatatt gtttcataaa ttttgccaaa ctcgaaattt ttgcttcatg attacccgct
      421 aaaccttgac aaaaagcttc ttgaaacaat gcatattcca catcaaattt accaatattt
      481 acagcaatag tataaaaacg tatagcttgt ttaaaatctt tgagcatata ataacagtcg
      541 cctgttctaa ttgcagcatc agaataaaaa tcgtcttttt tattatctgc aatatctaaa
      601 tatttacgaa accatgttgc agcatcggca aaacgttctt gctcaaaaaa agtatatgct
      661 atagaataat gagctctttt atattccggt gaattgaatg ccccgggcac aaggacaaac
      721 tcattaaata atgctcgagc tttatcatat tgcaaaagtt tataatagca ttcggctttc
      781 caatatatac tataggcctg aattgttctg tcaaaggttc catactgcat cgatttatca
      841 aacatttgaa ttgcttgtgt ataatcaata ttatttatca attctaaacc tctataatag
      901 gctatttgtt gatatgctat ttttaaatct gttgttaaaa ctttaatttg ttctaaagag
      961 gcaagagcat ctttataatt tttagatgac ataaaaattt taaccaataa actatttgcc
     1021 tcatctttat attttgatga cgggtattgt gccagaaaag attgagttgc agtaattgcc
     1081 tcattaaatg gagcatacga taattcataa atcaatttgg catgaataaa agtagcatct
     1141 tccttaatac ttggaaatac attatattgc atggcagcca taaaagcatt tcgtgcctta
     1201 tcttttattc ccaattttaa atagcaatct cctaaatgat agtaagcatt ttgtgcaatt
     1261 gtatcatttt gcgaagtaac ttgactgaat aaatcagcag ctttagcata tttaccatca
     1321 cgataataca aatagcctag ttcatacaaa tcttcttttg atggactcaa tgaggtctca
     1381 atatattttt cataatacgg taaagctaaa ttatattgca atgttttaaa atatgcacct
     1441 gctaaaactc tatatacttc aggtaattgt tgtggattta caattccttc aattgttctg
     1501 gcatattgaa ttaccttatc atattgctgt tgtttataat aaatttgaca aatatacggt
     1561 ggaactacag ttgaaaacat agtagaatca gcaaggaatt caaatccctt aagagctgag
     1621 gcataattct tatcataata ttctaaatat gaaaaataaa ataatgcatt ttgtgcatac
     1681 acagattttg aatcctttac ttctttaaaa aatggttttg ctcttgcgta atcattattt
     1741 ttaaaatatg aataccctat tttataaaaa tactcggaaa tctctgtatt attaaatttt
     1801 ttaatatcaa ccaactcata ccatataaga gcttcggggt agtgctgttc gcgaaacaaa
     1861 taattggcat actcaaaata tgcatgataa attcgagtac tttcaggatg cttttcaata
     1921 aattgtttaa tacgcatttc cgcatcacca ttgaacaatc gtaatgcaca tacagcaata
     1981 taatattcag catcggtacg tattgtttga gcgtaatctt ctgaagttga tattacaata
     2041 aatttttgtt gtgccgccgc aaatttttga ttatcaaata atgaaacagc ttcattaaaa
     2101 attctatttg attttgataa acgttcggta ttttgtgaaa acactaaagt tgttccggca
     2161 attatacaaa cggtaactaa aaaactttta attcggttca taatgtacaa tttaattcaa
     2221 gcaaaattct ctattttttt tgtgtttttt tttattatga ataatttttt ataaaaactt
     2281 ataaacgatg aaaaacacat ttaaatattt gttattcaaa tttttacatg tcaattttac
     2341 aatatacaac aacagttatt aacttgattt ttgataccat taaacggtaa actaacaatc
     2401 tattgtacat catatatata tttgctaaaa aaacatatga attattcaaa atatatagat
     2461 catagcttac tgcaccccac agcaacacac aatgatatag ccaaattatg ccgtgaagct
     2521 attgaatttg atgttgctgc agtatgtatt caacctattt atatacaatt tgcacagacc
     2581 ctattacaac acactcctat ttctgttgct acagttatag gatttcctca tggaacaaat
     2641 tctatagaaa cgaaatgttt tgaaacaaaa ttggcaataa aacatggtgc aaccgaaatt
     2701 gatgctgtta tttgcatacc acaagtaatt gcacaagaat ggaaatacat agaaacagaa
     2761 atacacgaat tatccacaat ttgtaaacaa cacaacattt gtcttaaaat aatttttgaa
     2821 acaacatact taaatgaata tcatataatt aaactatgcg aaatttgttc aaaagaacac
     2881 gtttcttttg taaaaacttc aacaggtttt gattttatta aacatgaatc cggattatat
     2941 accaccaaag gagctcaaat aagccatata caattaatga aaaaacattg tttcaacaat
     3001 actcaaatta aggcttcagg aggtattagg agcgcatcat attttataga atgtattgaa
     3061 gccggagcaa caagaattgg tacttctgca acaaaagata ttctaaaggc attaccataa
     3121 ttttataaaa aaaatatata tttgcacaaa aaaattgaga aacgtggtga agttatttac
     3181 aagacattta attactgtta tttccgcact gttattcaca tgtgtggtat atgcccaaga
     3241 cgaatttatg atacatggta gaatccaaac aatagataac tctattgaag gagcaacggt
     3301 tcgtgtaatg catgattatt taactataaa aaaaataatt ttagataaaa caggagcata
     3361 cactgtttat ttaccttatg gtaaaaatta ttcattatta tacgaaaaaa aagggtatca
     3421 aactattcaa attgacgcat tacttgattt accaaaagat gttaagcaat gttgttatcg
     3481 tccgttagaa ttatcttttc atttaacaaa accggattct actcatggcg atatgtttaa
     3541 caaagctttt catactattc aatacgacaa aaattataaa ggattcatat acgatataga
     3601 tattgattat atgattcaac aacgaatagt aaatgctcaa attttcaaac aaactctact
     3661 tgcagcacaa gaaggtaata gttcgcgaaa cgattcaata ttagttgaaa aaaaatattt
     3721 agcccttata aatcaaggaa ccgaatacta taatcaaaaa caattttatg ctgctcgaaa
     3781 attcttttta gaagcttcaa aaataaaacc tgaacgtatg tatccgcaat ataaacttga
     3841 agatattaaa acagaattac aacgatttga aactaaagct gaattgttag gaataaattt
     3901 agactcatta gttgcaaaag aattggccgc aattgagccg aaaattgaag aaaagaaacc
     3961 atatcctcca tatattccct taacagatgc acaagttgaa gaaatattta aaaatgattt
     4021 gaaaaaacaa attctcaaat caagttctaa tgttgctgaa gctaaccgaa ctattgcatt
     4081 aatgaatgaa ttattcaaag atgacatgaa aaaagttaca acagctgact ttaagaaaca
     4141 atcaaataaa gattctaata aacctgaaat tgacaaacgt gaagttggta taaaaccaaa
     4201 acaaacaaac aaacaagttg aagttgcaaa agctactaca gaaactaaaa aagttgaaga
     4261 agttgttcaa aaaccaaaag ctaccgaacc aaataaagaa gaccaacaag aaaaaattca
     4321 agatatagca gatgttataa ccagcaaacc tgagaaaaaa cctattacaa caaaagaaac
     4381 aacagaaaaa cctgtaaaaa aggtagttga ttataatacg tatcaagatt cattacgtgc
     4441 tcgctaccct gatgaacgaa caatagaagt tactcaagat gctcataaaa aaactactcg
     4501 tgtatttatg aacaatggaa aaatagttga aatatatgct atggtagaac atgcatgggg
     4561 agcaacatat tattttatag aagaatatcc ttcaggtgct caaagcattg gatatgcagc
     4621 ttttatgagc cgaactaaat tatatgattt acaaaataat aaacctgcag aaataaaaga
     4681 ttctgttccg gcacagaaaa aataaattat aatttctatt tttttagaat attatagtat
     4741 tttgcaaaaa aaaagaacta tggaagattc atcgttgaat ccagaaacat gggtagattt
     4801 atatggtaa
//
LOCUS       DAOTOW010000073         4774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14684_length_4774_cov_3.709472, whole genome shotgun sequence.
ACCESSION   DAOTOW010000073 DAOTOW010000000
VERSION     DAOTOW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14684_length_4774_cov_3.709472"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1272)
                     /gene="trpB"
                     /locus_tag="P5243_01920"
     CDS             complement(85..1272)
                     /gene="trpB"
                     /locus_tag="P5243_01920"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRS18234.1"
                     /translation="MKYTIDEHGYYGKFGGAYIPELLYPNIKELEDNYIRIIESESFK
                     SEFKELLKDYVGRPSPLTYAKRLSEKYNTHIYLKREDVNHTGSHKINNTIGQILLAKA
                     LGKKRIIAETGAGQHGVATATVCALMGMQCTVHMGETDVKRQMPNVLRMKMLGATVVP
                     AMSGNRTLKDAGNEAFRDWINNPTETHYLLGSVVGPHPYPDMVARFQAVISEEIQWQL
                     QEKTGNTLPNYVIACIGGGSNAMGAFYHFLEHESVQLIAVEAAGLGVNSGETAASIAK
                     GSIGVIQGAKTMLMQTNDGQIIEPYSISAGLDYPGIGPAHAHFHDIGRTTVLNATDHE
                     ALQAAFELTRLEGIIPAIESAHALAALPKIQFTKNDIVVVNISGRGDKDMDTYIANMD
                     TYC"
     gene            complement(1307..2290)
                     /locus_tag="P5243_01925"
     CDS             complement(1307..2290)
                     /locus_tag="P5243_01925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRS18235.1"
                     /translation="MKFTTEVAIPPPLCTITHSDVGLAIGSCFTTYIGERLQQLKFPI
                     LVNPLGTIYNPFSIAQSIDILLGNKQIQDEDLFLEQGVWQSLYLHTKYSSRNKEDIKQ
                     NIAHLQTEFSITLPQLQYIIISLGTAWVYKHKQLGCIVNNCHKLPAHNFERYRLEPKE
                     IVTILANTIELLRSKTNTELHIIFTVSPIRHTKDGAFGNNVSKAALLLAIDELLELPN
                     TYYFPAYEIVLDELRDYRFYAQDMIHPSDTAVAYIFEKFQSTMCNEQTRTMCKTIESI
                     NKAIQHKPFNNTNPEYTKFISNTIEECKLIMQTHTKIDLNYEIELLQNRLL"
     gene            complement(2295..3824)
                     /gene="gltX"
                     /locus_tag="P5243_01930"
     CDS             complement(2295..3824)
                     /gene="gltX"
                     /locus_tag="P5243_01930"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRS18236.1"
                     /translation="MTQKKIRVRFAPSPTGPLHMGGVRTALFNYLFARKHGGDFLLRI
                     EDTDQTRFVRNAEKYIVDSLTWCGITIDEGIGAVNQGEYGPYRQSERKNLYRNFAYDL
                     IASGNAYYAFDTAEELNTLREQGEAAKNPFVYNFETRKHLCNSLTLSEQEVAQRIADN
                     QPYVIRFKMPENQDVHLYDEIRKEVHFNTKELDDKVLYKSDGMPTYHLANVVDDYTMG
                     ITHVIRGEEWLSSMPLHVLLYRAFGWEADMPKFAHLPLILKPQGQGKLSKRDGDKLGF
                     PVFPLNWVGDDGETAAGYKETGYLPEAFVNMLALLGWNPGTEQEIFTMDELIDAFSLE
                     RVGKSGSKFDPEKTKWFNQQWIQRTPNYKLAELILPTILEQGIQTSPQYVEKVCALVK
                     ERAVFIQDLWLQATYFYKAPETYDEKVIQKRWDSSIPAILQEICEILQTQNTFSSAHL
                     EETIKQYIDNKQLNMGQIFNCIRLCIVGTSAGAHLFDIFEMIGKTETIARIQKAIATI
                     H"
     gene            4056..4484
                     /locus_tag="P5243_01935"
     CDS             4056..4484
                     /locus_tag="P5243_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18237.1"
                     /translation="MITLELTETQMQDLLQMVYLGEWMTQAYGNSEHAQDYEELIQKL
                     YATAYNNGIESDVEFDKKMGGYVPTNEFESFCDDIISQYDEHTFWEELIMRMATKELQ
                     SQGGGQLSSAEYEKQLQKLIEKYEKECNTHGIENLSFSQK"
     gene            4481..>4774
                     /locus_tag="P5243_01940"
     CDS             4481..>4774
                     /locus_tag="P5243_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRS18238.1"
                     /translation="MRGTVIKSTGSWYKVVADNNQVYDCRIKGKLRLDDTKSTNPIAV
                     GDHVEFVPDAETQGVIAEILERKNYIIRKASNLSKQSHILAANIDRAYLVVTLH"
BASE COUNT         1411 a          770 c          883 g         1710 t
ORIGIN      
        1 tgctgtaaaa tataccgaaa gtatgttgga ttgtttttgg gtaaaaagtt gttgtatgcg
       61 attcatatgt atgcgtaata cagtttaaca ataagtatcc atgttggcaa tataggtatc
      121 catatcttta tcgcctcgtc ccgaaatgtt tacaacaaca atatcgtttt ttgtaaattg
      181 tattttgggt aaagcagcca gtgcgtgagc cgattctatg gcaggtatta ttccttcgag
      241 tcgcgttaat tcaaaagctg cttgtagtgc ttcatggtcg gttgcattga gtacggttgt
      301 tctacctata tcatgaaagt gagcatgtgc cggcccaatg ccgggataat ctaatcctgc
      361 cgaaatagaa taaggttcta ttatttgacc gtcgttggtt tgcataagca tggttttggc
      421 accttgtatt actcctatag aaccttttgc aatggaagct gccgtttcgc ccgaatttac
      481 tcctaaacct gccgcttcta cagcaattaa ttgaaccgat tcgtgttcta aaaaatgata
      541 aaaagcaccc atggcattac tgccgccacc tatacaggct attacataat ttggtaatgt
      601 attaccggtt ttttcttgta attgccattg aatttcttcg cttattacag cttgaaatcg
      661 ggcaaccata tcggggtaag ggtgtggtcc tacaaccgaa cccaataaat aatgagtttc
      721 tgtaggattg ttaatccaat cacgaaaagc ttcatttcct gcatctttta atgttctatt
      781 accactcata gcaggtacta cggtagctcc caacattttc atacggagta cattgggcat
      841 ttgtcgtttt acatcggttt cacccatatg taccgtgcat tgcattccca tgagagcaca
      901 tactgttgct gtggcaactc catgttgtcc tgcgccggtt tctgctatta ttcgtttttt
      961 tcctaatgct tttgccaata gtatttggcc tattgtatta tttattttgt gtgagccggt
     1021 gtggtttacg tcttcacgtt ttaaatatat atgagtgttg tacttctctg acaatcgttt
     1081 ggcataggta agcggtgagg ggcgaccaac atagtctttg agtaattctt taaattctga
     1141 tttaaatgat tctgattcaa ttattcgaat ataattatct tcaagttctt ttatattcgg
     1201 atatagtaat tcgggaatat atgctcctcc aaatttgccg taataaccgt gttcgtcaat
     1261 agtatatttc atgtattttt tttttacaaa tatagtagat tatatcctat aacaatctgt
     1321 tttgaagtaa ttcaatttca tagtttaaat caatttttgt gtgagtttgc attatgagtt
     1381 tgcattcttc tattgtattt gatataaact ttgtgtattc cgggttggta ttgttaaatg
     1441 gcttgtgttg tatagctttg tttattgatt ctatagtttt acacattgtg cgcgtctgct
     1501 cattacacat tgtagattga aatttttcaa aaatgtaagc aactgcagta tccgatggat
     1561 gaatcatatc ttgtgcataa aatctataat cacgcagttc atctaataca atttcatatg
     1621 ccggaaaata atatgtgttt ggtaattcta ataattcatc aattgctaat agtagagcgg
     1681 ctttactgac attgttgcca aatgcaccat cttttgtatg tcttataggg cttaccgtaa
     1741 aaattatgtg taattctgta tttgttttcg aacgcaataa ttcaattgtg tttgcaagta
     1801 ttgtaactat ttctttaggt tctaatctat aacgttcaaa attgtgtgca ggtagtttgt
     1861 gacaattgtt tacaatacaa ccaagttgtt tgtgtttata tacccatgca gtaccaagac
     1921 ttataataat atattgtaat tgtggtagag taatagaaaa ttctgtttgt agatgtgcta
     1981 tgttttgttt aatatcttct ttgtttctac tactgtattt tgtatgtaaa tataatgatt
     2041 gccatacacc ttgttccaaa aataaatctt catcttgtat ttgtttgttc ccaagtaata
     2101 tatcaatgct ttgtgctatt gaaaaaggat tgtaaatagt tcctaatggg tttacaagta
     2161 tgggaaattt tagttgttgt aatcgttcac cgatataggt tgtaaaacat gaaccaattg
     2221 ccaatccaac atcactatgc gtaatagtac aaagaggtgg gggaatggca acttcagttg
     2281 taaatttcat gtttttaatg tattgtagct atagcttttt gtatgcgggc tatagtttcg
     2341 gttttgccta tcatttcaaa aatatcaaat aaatgagcac ctgcactagt gccaactata
     2401 cacaagcgaa tacagttaaa tatttgtccc atattcaatt gtttgttgtc tatgtattgt
     2461 tttattgttt cttctaaatg tgctgaacta aaggtgtttt gagtttgcag aatttcgcat
     2521 atttcttgta aaattgcagg tattgatgaa tcccaccgtt tttgaatcac tttttcgtca
     2581 tatgtttcag gggctttgta aaaataagta gcttgtaacc ataaatcttg aataaaaaca
     2641 gcgcgttctt ttactaaagc acatactttt tcaacgtatt gaggtgaggt ttgaattcct
     2701 tgttctaata tggttggtag tatgagttcg gcaagtttat agttaggtgt gcgttgtatc
     2761 cattgttgat taaaccattt tgttttttcg ggatcaaatt ttgaccctga ttttcctacg
     2821 cgttctaaac taaaagcgtc aattagttcg tccatagtaa aaatttcttg ttcggttccg
     2881 gggttccacc caagtaaagc aagcatgttt acaaaagctt cgggtaaata gccggtttct
     2941 ttatatcctg ctgctgtttc accatcgtcg ccaacccaat taagcggaaa tacaggaaaa
     3001 cctaatttat cgccatcgcg tttacttagt ttaccttgtc cttgtggttt taatatgagc
     3061 ggtaaatgtg caaattttgg catgtcggct tcccaaccaa aagcacgata caaaagtaca
     3121 tgtaaaggca tactcgaaag ccattcttca cctcgaatta catgtgttat tcccatagta
     3181 tagtcatcaa ccacatttgc taaatggtag gtaggcatac cgtctgattt atacaaaact
     3241 ttgtcgtcaa gttcttttgt gttaaaatgt acttctttac gaatttcgtc atacaaatgt
     3301 acatcttggt tttcgggcat tttaaatcga attacatatg gttggttatc tgcaattctt
     3361 tgagcaactt cttgttcgct tagtgttagg gaattacaaa gatgtttgcg tgtttcaaaa
     3421 ttgtatacaa aagggttttt ggcagcttca ccttgttctc gtaaggtatt gagttcttcg
     3481 gcagtatcaa aagcataata agcattaccc gaagctatta agtcgtatgc aaaatttcgg
     3541 tatagatttt ttcgttcact ttgtctgtat ggtccatatt ctccttgatt tacagctcct
     3601 atgccttcat ctattgttat accacaccac gtcaaagaat ctactatata tttttctgca
     3661 tttcgtacaa atcgagtttg atcggtatct tctatacgta ataaaaagtc gccaccgtgt
     3721 ttacgtgcaa ataaatagtt gaataatgct gtgcgtactc cacccatatg gagcggaccg
     3781 gtaggacttg gagcaaaacg aactcgaatt tttttttgtg tcatagttaa ttttttttgt
     3841 tgagcaaata taaaaaaaaa tgattacaga atactgttag ataataagct gataatttaa
     3901 tctgtaataa gagtatgagt tttaataaag gctattagtt attttgttgt agtgtaaaaa
     3961 aagaatagtt tttcatatat caagaatttc ttcttagttg gttatagcaa aaacttagac
     4021 gtaaaaaact gtatatttgt aaaaaaaata gaattatgat tactttagaa cttacagaaa
     4081 ctcaaatgca agatttgttg cagatggtat atttgggcga atggatgact caagcctatg
     4141 ggaatagtga acatgcacaa gattatgagg agttgattca aaaattatat gcaactgcct
     4201 ataataatgg tatagaaagc gatgttgaat ttgacaaaaa aatgggagga tatgttccta
     4261 ccaatgaatt tgaatcgttt tgtgatgata taatttcaca atatgatgag catacgtttt
     4321 gggaagaact tattatgcgt atggctacca aagaattaca atcacaagga ggggggcaat
     4381 tatcatcggc cgaatacgaa aagcaattgc aaaaacttat tgaaaaatat gaaaaagaat
     4441 gtaataccca tggtattgaa aatctatcat tttcacaaaa atgagaggta ctgttataaa
     4501 atcaaccggc agttggtata aggttgttgc agataacaat caggtgtatg attgtcgcat
     4561 aaaaggaaaa ttgcggcttg atgatacaaa aagtactaat ccaattgccg ttggcgacca
     4621 cgttgaattt gtccccgatg ctgaaacaca aggggtaatt gcagaaattt tggagcgtaa
     4681 aaattatata attcgaaaag catcaaattt atcaaaacag tctcatatac tagctgcaaa
     4741 tattgacagg gcatatttgg ttgttacttt gcat
//
LOCUS       DAOTOW010000074         4765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14724_length_4765_cov_3.514862, whole genome shotgun sequence.
ACCESSION   DAOTOW010000074 DAOTOW010000000
VERSION     DAOTOW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14724_length_4765_cov_3.514862"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="P5243_01945"
     CDS             complement(<1..1922)
                     /locus_tag="P5243_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRS18239.1"
                     /translation="MAQNKLFLIDGHAMLYRSYFAFIKNPRINSKGVNTSGIFGFVNT
                     LFDVIQTQKPTHIGVVFDPKGDTFRHTMYKLYKANRQETPEDLRASIPHVMEIIQGMN
                     IPVIQIEGYEADDVIGTLAKQAAKQGFEVYMATPDKDYCQLVQDNIYIYRPRSFGAGI
                     EILGVPEVLQKFEVSSPQQVIDILGLWGDSSDNVPGAPGIGEKTAKSLIAQYGSIEGI
                     YQHIDELKGKQKESLLANKELVMLSKQLVTIKIDVPIDFSSYNFVKEEFNVGILQEKF
                     KELEFRTLLDRLIPQRTVQNNIKPAPAQQSLQLGLFDEPSEQPVYVSSYQTINTVNHS
                     YFLVDNQSHIQKLIQELLLQKEFCFDTETTSLNTLEAELCGIAFSYEAHKAYYVPFPE
                     NQQEAFTLAQQFAPVFSSKSLKIGQNLKFDMSVLANYNITIAGPVFDTMIAHYIIQPE
                     GRHKLESLSEQYLNYEMVKIEELIGKKGKNQGSFRDVPLEQAKEYAGEDADITFQLYQ
                     LLSKELKQKNQLELCETIEFPLIEVLNTMERNGTCIDSVALQEFSDMLSTELQKVEKQ
                     IYQLAGTSFNIGSPKQLGEILFDTLAIVDKAKKTKTKQYATGEDVLEKLLDAHPIVPL
                     ILEYREYKKLLSTYVDA"
     gene            complement(2022..3827)
                     /gene="uvrC"
                     /locus_tag="P5243_01950"
     CDS             complement(2022..3827)
                     /gene="uvrC"
                     /locus_tag="P5243_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRS18240.1"
                     /translation="MNNPTPYERCKNLVGILPEKPGVYQFFDKHDTLLYVGKAKNLKK
                     RVTSYFVKEHEHAKTRVLVSKIHSIQHIVVNSEQDALLLENNLIKKYRPRYNIMLKDD
                     KTYPWIVIKNEPFPRVFHTRTMVKDGSTYFGPYANVRMSRFLLDMIFQLYTLRTCSLN
                     LSNTEIAKGKYKVCLEYHIKNCKGPCVGLENEEAYNQTINDIKNILRGNIHEVIQVLT
                     KKMKLHAEALQFEEAKQIKEHIDALARYQSKSTIVSPSITNVDVYSIEDDIDVAYVNF
                     LKVVNGGVIQVHTIEIKKQLDETPEELLGMAITEIRDKIPSNAHEIIVPFMPDFLLEH
                     VTYTIPQRGDKKQLLELSQRNVTYYKLEKLKQLKRVDPERHSNRILETIQKDLRLSEL
                     PVHMECFDNSNIQGAFAVAACVVFKNAKPSKQDYRHFNIKTVEGPNDFASMEEVLTRR
                     YTKLIAENEPLPQLVIVDGGKGQLSSAVKVFTQLGIVDKVKLIGIAKRLEEIFFPGDS
                     IPLYLNKTSETLKVIQHMRDEAHRFGITHHRNKRSKHFIQSELTSIQGIGSTIAEKLL
                     SHFKSIEAIKNASEEELTKLIGKSKTSVIKAYFHA"
     gene            complement(3820..>4765)
                     /locus_tag="P5243_01955"
     CDS             complement(3820..>4765)
                     /locus_tag="P5243_01955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS18241.1"
                     /translation="CDTAKLKTVLNSADVVFLPSFTHNSVQFIRKHTNKPICFDPLIS
                     KYLTKVFDYKLVWKYSPRALKNFYKDKRAFSACDFMIADTVAHKSYYARTFKISHERI
                     FVLPVGVNTNDFAPCTTHKHSNNTFTIGFYGGFIPLQGVSNIIDAAHILRQNANVRFE
                     LVGNGFEFEAMKKKAATLGLHNITFMGWQPYTDLPAIINSWDICLGIFGNTQKADLVI
                     PNKIYHYAAMQKPIISKHSEAIQELFTHNESILLCSTQPHDIASSITELIENHEKRNT
                     LGIHALQVAQQYNHIQTATQFIRILEQGLQYLQATKHE"
BASE COUNT         1403 a          740 c          800 g         1822 t
ORIGIN      
        1 gcatcaacat aggttgataa taattttttg tattctctat attctaaaat aagaggaaca
       61 ataggatgag catctaaaag tttttcaagt acatcttcgc cggttgcata ttgttttgtt
      121 ttagtttttt ttgctttgtc tactattgcc aatgtgtcaa ataatatttc acctaattgt
      181 tttggtgaac ctatattaaa ggaggttccg gctaattggt atatttgttt ttctactttt
      241 tgcaattctg ttgacagcat gtcagaaaat tcctgtaaag caacagaatc tatacaagtt
      301 ccgttgcgtt ccatagtatt caatacttct attaatggaa attctatggt ttcacataat
      361 tctaattgat ttttttgttt caattcttta gaaagtaatt gatataattg gaatgtaata
      421 tcagcgtctt ctccggcgta ttcttttgct tgttcaagcg gtacatcacg aaatgaacct
      481 tgatttttac cttttttacc aataagttct tcaattttta ccatttcata attcaggtat
      541 tgttccgata gagattctaa tttatgtctt ccttcgggtt gtattatgta gtgtgcaatc
      601 atagtgtcaa acaccggccc tgcaattgtt atgttgtaat tggctaaaac cgacatatca
      661 aatttcaaat tttgtccaat tttcaatgat tttgatgaga atacaggagc aaattgttgt
      721 gctagagtaa aagcttcttg ttgattttcg gggaatggca cataataggc tttgtgtgct
      781 tcatatgaaa aagctattcc acatagttct gcttctaatg tgttgagaga ggtagtttcg
      841 gtatcaaaac aaaattcttt ttgtagcagc aattcttgaa ttaatttttg aatatgagat
      901 tgattatcta ctaaaaagta actatgatta acagtattaa ttgtttgata cgatgaaaca
      961 tatactggtt gttcagatgg ttcatcaaat aaacccaatt gtaaagactg ttgagcagga
     1021 gccggcttta tattgttttg aacagttctt tgcggtataa gtctgtctaa aagagtgcga
     1081 aattcaagtt ctttgaattt ttcttgtaaa attccaacat tgaattcttc ttttacaaaa
     1141 ttgtatgatg aaaagtcaat ggggacatca atttttatgg ttactaattg tttagaaagc
     1201 attactaatt ccttattagc aagcaaactt tctttttgtt tcccttttaa ttcatctata
     1261 tgttggtaaa taccttcaat agaaccatat tgagctataa gtgattttgc tgttttttca
     1321 ccgattcccg gagctcccgg aacattatca gagctgtcac cccaaagtcc aagtatatct
     1381 attacttgtt gtggggaaga cacttcaaat ttttgtaaca cttcgggtac tcctaatatt
     1441 tctatacctg caccaaaact gcgaggccta tatatatata tgttatcttg aaccaactga
     1501 caatagtctt tatcaggagt tgccatgtat acttcaaatc cttgttttgc agcttgttta
     1561 gccagtgtgc caattacatc atctgcttca tatccttcta tttgaattac cggaatattc
     1621 atgccttgta ttatttccat aacatgagga atagatgctc gtaaatcttc gggagtttct
     1681 tgtcgatttg ctttgtatag cttatacata gtgtgtctaa atgtgtcacc ttttgggtca
     1741 aatactactc caatatgagt tggtttttgt gtttgaatta catcgaacaa tgtatttacg
     1801 aatccaaata ttcctgatgt gttaacgcct ttactgttga tgcgtggatt tttaataaat
     1861 gcaaaataag aacggtacaa cattgcatga ccgtcaataa gaaataattt gttttgagcc
     1921 atagtaataa tttattattg taaagatatg atttttatac aaatactact atttttttac
     1981 tacaaaacca attcattaca atatgtgatg aaaaaagagt attaggcatg aaaatatgcc
     2041 ttaataacag aagttttaga ttttcctatg agtttggtta attcttcttc tgaagcgttt
     2101 tttattgctt ctatggattt aaagtgtgat aaaagttttt cggcaattgt gctaccaata
     2161 ccttgaatag aggttaattc agattgtata aaatgttttg aacgtttatt tcggtggtgt
     2221 gttattccaa acctgtgtgc ttcatctcgc atgtgttgta taaccttaag tgtttcgctg
     2281 gttttgttta aatataatgg tatagaatct ccggggaaaa atatttcttc taagcgttta
     2341 gctataccta tgagtttaac tttgtctaca atacctaatt gagtaaatac ttttacagca
     2401 gaacttaatt gacctttacc tccgtcaact attaccaatt ggggtaacgg ttcattttcg
     2461 gcaattaatt ttgtatatcg tctggtaaga acctcttcca ttgatgcaaa atcattgggg
     2521 ccttcaacag tttttatatt aaaatgtctg taatcttgtt tacttggttt ggcattttta
     2581 aatactacac aggcggcaac agcaaatgct ccttgaatat ttgaattgtc aaaacattcc
     2641 atgtgtacgg gtagttcaga aagacgtaaa tctttttgta tggtttctaa aattctatta
     2701 ctatgtcgtt caggatcaac tcgtttaagc tgttttaatt tttctaattt ataataggtt
     2761 acattgcgtt gcgaaagttc aagtaattgt tttttgtcgc ctcgttgcgg aatagtatag
     2821 gtaacatgtt ctaataaaaa gtcgggcata aaaggaacaa ttatttcatg tgcattactc
     2881 ggaatcttgt ctcttatttc agtaatagcc attcccagta attcttcggg agtttcgtct
     2941 aattgctttt taatttcaat agtatgaact tgaattacac ctccgtttac taccttaaga
     3001 aaatttacat atgctacgtc tatatcatct tctatagaat atacgtctac attggtaata
     3061 cttgggctta caatggtaga tttgctttga tatcgtgcca atgcatctat atgttcttta
     3121 atttgttttg cctcttcaaa ttgtaatgct tcggcatgaa gtttcatctt ttttgttaga
     3181 acttgtatta cttcatgaat attcccacgt aaaatatttt tgatatcgtt tatagtttga
     3241 ttgtaggctt cttcgttttc taaacctacg catggccctt tgcaattttt aatgtgatat
     3301 tctaagcaaa ctttatattt cccttttgct atttctgtat tgcttaaatt gagtgaacat
     3361 gtacgcaaag tgtataattg aaaaatcata tcgagtaaaa aacgagacat gcgaacattt
     3421 gcataaggtc caaaatatgt agaaccatct tttaccattg tgcgtgtatg aaaaacgcga
     3481 ggaaacggtt catttttaat tacaatccac ggatatgttt tatcgtcttt gagcattata
     3541 ttgtatcgag gacgatattt ttttatcaaa ttattttcga gtaataaagc atcttgttca
     3601 ctatttacca ctatatgttg tatagaatgt attttgctta ccagtacccg tgttttggca
     3661 tgttcatgtt cttttacaaa atatgatgtt actcgttttt ttaaattttt agctttgccc
     3721 acatacagta acgtatcatg tttatcaaaa aactgatata caccaggttt ttctggtaaa
     3781 attcctacta agtttttaca acgttcgtat ggtgttgggt tattcatgtt ttgttgcttg
     3841 taaatattgt aatccttgct ccaagatgcg tataaactgt gttgctgttt gtatgtggtt
     3901 atattgttgg gctacttgta atgcatgtat tcctaatgta tttctttttt catgattttc
     3961 tattaattcg gtaatgcttg atgctatatc gtggggttgg gtagaacata ataatatgga
     4021 ttcattatgt gtaaataatt cttgtatagc ttcgctgtgc ttacttataa taggtttttg
     4081 catagctgca taatggtaaa ttttatttgg aataactaaa tcggcttttt gggtattacc
     4141 aaaaatacct aaacaaatat cccatgaatt aataattgca ggtaaatcgg tgtatggttg
     4201 ccaccccata aatgtaatat tgtgtaaccc aagtgtagca gctttctttt tcatagcttc
     4261 aaattcaaaa ccattgccta caagttcaaa tcgtacattg gcattctgtc ttaaaatatg
     4321 agccgcatct attatattag aaactccttg taaaggaata aatccaccat aaaaccctat
     4381 tgtgaatgta ttgtttgagt gtttgtgtgt agtgcaaggt gcaaaatcat ttgtattaac
     4441 tcctacaggt aacacaaaaa ttcgttcgtg tgatatttta aatgtgcggg cataatatga
     4501 tttgtgtgca actgtatcag caatcataaa atcgcaggca gaaaatgccc gtttgtcctt
     4561 gtaaaaattt tttaaagcac gcggagaata tttccaaacc agtttataat caaagacttt
     4621 agtcaaatat ttagaaataa gaggatcaaa gcaaataggt ttgttagtat gctttcgaat
     4681 aaattgtaca ctattgtgag taaatgatgg taaaaataca acatcggcac tatttaaaac
     4741 tgttttgagc ttggcagtat cacat
//
LOCUS       DAOTOW010000075         4749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14795_length_4749_cov_3.348743, whole genome shotgun sequence.
ACCESSION   DAOTOW010000075 DAOTOW010000000
VERSION     DAOTOW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14795_length_4749_cov_3.348743"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..834)
                     /locus_tag="P5243_01960"
     CDS             complement(220..834)
                     /locus_tag="P5243_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRS18242.1"
                     /translation="MKFVRNIYDWVLAWAESKYGPLALFILAFAESSFFPIPPDVLLI
                     ALAIGATQKSFRFALNCTIASVLGALAGYSIGHFAWIGSNGEFTSFANFFFTHIPGFT
                     HEIYEKIQLMYQEWDFWVIFTAGFTPIPYKVFTITSGVFDINLLMFILASIISRGARF
                     FLIAFLIWKYGASIKAFIDKYFNILAVGFTVLLIGGFVLIKYLV"
     gene            complement(930..2192)
                     /gene="nqrF"
                     /locus_tag="P5243_01965"
     CDS             complement(930..2192)
                     /gene="nqrF"
                     /locus_tag="P5243_01965"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRS18243.1"
                     /translation="MILFDASAGTVIGFSILVFLLIIILLVAMLLFAKSKLMPSGEVS
                     FKLNEEEVRKVKPGGTLLNTLQAQSVLLPSACGGSGSCGMCRCKVYQGGGDILPTEKG
                     FFSRKEQQEKWRLACQVKVKEDMEVWVPEEVMGVKKWECEVISNKNVASFIKEFVVKL
                     PEGEILNFKSGGYIQIDVPKYECDFSKDIEVDDKYKDEWDKFKLWDLKIKNNEETFRA
                     YSMANHPAEGNIVMLNIRIATPPWDREKGTWKNVPPGVCSSYIFSRKPGDKVTISGPF
                     GEFFIQETNNEMLYIGGGAGMAPLRSHLFHLFHTVKTGRKVSYWYGARSKREIFYEEH
                     FRAIEKEFPNFTFNIALSEPLPEDNWTGYVGFIHQVIYDEYLSKHEAPEDIEYYMCGP
                     GPMTNAVNKMLDNLGVARESIYFDDFGN"
     gene            complement(2213..2830)
                     /gene="nqrE"
                     /locus_tag="P5243_01970"
     CDS             complement(2213..2830)
                     /gene="nqrE"
                     /locus_tag="P5243_01970"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRS18244.1"
                     /translation="MEHLLDIFVRSIFIDNMVFAYFLGMCSYLAVSKNVSTAAGLGIA
                     VTFVLVITVPVNYLLNEFVLKEGALVWLHASFKELDLSFLSFIMFIAVIASMVQLVEM
                     VVEKYSPSLYSSLGIFLPLIAVNCAILGGSLFMQEREYQSLAEATVFGFGSGLGWLLA
                     IVGIAAINEKIKYSHVPPALKGLGIAFLVTGIMGIAFMSFMGIKL"
     gene            complement(2837..3493)
                     /locus_tag="P5243_01975"
     CDS             complement(2837..3493)
                     /locus_tag="P5243_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRS18245.1"
                     /translation="MSADKEPLFSKKNIRLLTGPLGAENPITVQSLGICSALAVTVKV
                     EPAIVMSLSVTIVLAFANVIISLLRKTIPDRIRIIVQLIVIAALVIIVDQILKAYAYD
                     ISKQLSVFVGLIITNCILMGRLEAFALGNKPWPSLLDGIGNGLGYGIILVLVSVVREI
                     FGSGTVTLPGIGELHVIPHGLYDFGYVNNGLMLLSPMALFVVGIIIWIQRSRNKELIK
                     "
     gene            complement(3498..4229)
                     /gene="nqrC"
                     /locus_tag="P5243_01980"
     CDS             complement(3498..4229)
                     /gene="nqrC"
                     /locus_tag="P5243_01980"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HRS18246.1"
                     /translation="MERSNSYIFTYSVIMVVLVAALLSIASYSLKDTQKSNVRKEKMQ
                     NILSSIQVESTTDNVEELYAKYIVNSAIIDINGNVIPYVLVPNGKLTDTIRAFDIDMK
                     KLYAMPRAQRTLPLFEAQKDGKKYIIIPMRGKGLWGPIWGFISFESDNNTVYGAVFDH
                     QGETPGLGAEINKDKFEHMFRGKQIFDTSGTFISIKVIKGGAAPDNIHGVDAISGGTI
                     TSNGVNAMLDTCLSTYVSYLQSIKQ"
     gene            complement(4234..>4749)
                     /locus_tag="P5243_01985"
     CDS             complement(4234..>4749)
                     /locus_tag="P5243_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRS18247.1"
                     /translation="AYSGFVDNQLSTFDLLVGNYAGSVGETSVIAIAIGAIILLYTGI
                     ASFRIMFSVFAGGFLMASLLNAVAPAGNILMNTPALEHMFIGGFAFGAVFMATDPVTA
                     AQTGKGKWIYGFLIGAFAIIIRCLNPAYPEGMMLAILFMNVFSPLIDHVVIQSNIKKR
                     LRRAKVSQLNK"
BASE COUNT         1644 a          970 c          661 g         1474 t
ORIGIN      
        1 aaatatcttt gttacattat aattaaacac tgtcctaaaa atgggaagct tacattttca
       61 ccacagataa cacagatttt cacagataaa caaatcaatt aattcctcta taccttaaac
      121 attagacctt atagcctttg ttcactataa attacacaaa ttttcacaaa agaatagagt
      181 aaaaataaaa ttccttattt ttaaatcgtc aataaaacat cacaccaaat acttaatcaa
      241 cacaaaaccg cctatcaaca ataccgtaaa acctacggca aggatattaa agtatttgtc
      301 gataaatgct ttgatgcttg ctccgtattt ccaaataagg aatgcaatta agaaaaatcg
      361 tgcgccgcgg cttataatag aagcaagtat aaacataagc aagttaatat caaatactcc
      421 tgatgttatg gtaaatactt tgtacggtat aggagtaaat cctgctgtaa aaatcaccca
      481 aaaatcccat tcttgataca tcaattgtat tttttcgtag atttcgtgag taaacccggg
      541 aatgtgtgtg aaaaagaagt ttgcaaacga ggtaaattct ccattgctgc ctatccatgc
      601 aaaatgccct atagaatatc ctgccaaagc acccaatacc gatgctatag tgcaatttaa
      661 cgcaaatcta aatgactttt gtgtagctcc tattgccaaa gctataagca atacatcggg
      721 tggtatagga aaaaatgatg attcagcaaa tgctaatata aacaatgcta agggaccata
      781 ttttgattct gcccatgcaa gtacccagtc gtaaatattt cgtacaaatt tcatatcaca
      841 cttttttata taaaaacacc ttatactatt gaagtataag gtgcaaaact aatgtatttt
      901 accttgtaaa ctttactatt cctaaaaaat taattaccaa aatcatcaaa ataaatactt
      961 tcgcgagcaa caccaagatt gtcgagcatt ttgttcactg cattggtcat agggccaggt
     1021 ccgcacatgt agtactctat atcttctgga gcttcgtgtt tgcttaggta ttcatcgtat
     1081 attacctgat gtataaatcc tacatatcct gtccaattat cttcgggcag tggttcactt
     1141 aatgcaatat tgaatgtaaa attagggaat tctttttcaa ttgcacggaa gtgttcttca
     1201 tagaaaattt cgcgtttact tcgtgctcca taccaatacg atactttacg acctgtttta
     1261 acagtatgaa acaaatggaa taaatgcgaa cgcaatggag ccataccggc accaccacct
     1321 atatacaaca tttcattatt tgtttcttga ataaaaaatt caccaaatgg tccggaaata
     1381 gtaactttat cacccggttt acgtgagaaa atatatgacg aacatactcc cggaggcaca
     1441 tttttccatg ttcctttttc tctgtcccat ggcggggtag ctattcgaat attgagcatt
     1501 acaatattac cttcggcagg gtgattggcc atagaataag cgcgaaaagt ttcttcgttg
     1561 tttttaattt ttaaatccca caatttgaat ttatcccact catctttata tttatcatca
     1621 acttctatat cttttgaaaa atcacattca tattttggta catctatttg tatgtatcca
     1681 cccgatttaa aattgagtat ttctccttca ggcaatttca caacaaattc ttttataaat
     1741 gacgcaacat ttttatttga aataacttcg cattcccatt tttttacacc cattacttct
     1801 tcgggaaccc atacttccat gtcttctttt actttaacct gacatgccaa acgccatttt
     1861 tcttgttgtt ctttacgact aaaaaaacct ttttcggtag gtaatatatc gccgccgcct
     1921 tggtacactt tgcaacggca cataccacac gaaccactgc caccacatgc cgaaggcaat
     1981 aatacagatt gagcttgcag cgtatttaac aacgtccctc cgggttttac tttgcgtact
     2041 tcttcttcgt ttaatttaaa agaaacttct cccgatggca ttaatttaga tttggcaaac
     2101 aacaacattg ccaccaacaa tataataatc aacagaaata ccaggatact aaatcctata
     2161 actgttccgg ccgatgcatc aaatagtatc attatgtata tcaatattta tattataatt
     2221 taattcccat aaaactcata aaagctatac ccataatgcc tgttaccaaa aaagcaatac
     2281 caagaccttt aagtgcagga ggcacatgcg aatatttaat tttttcgtta attgcagcta
     2341 tacctacaat tgccaacaac caaccaagac cagaaccaaa gccgaatacg gtagcttctg
     2401 ccaaactttg atattcacgt tcttgcataa acaatgaacc acccaaaatt gcacaattta
     2461 cagctataag tggcaaaaat atacccaacg atgaatatag tgaaggagag tatttttcaa
     2521 ctaccatttc aactaattgc accatagaag ctattactgc aataaacata ataaaactta
     2581 gaaaactcaa atctaattct ttgaaacttg catgtaacca taccaatgca ccttctttga
     2641 gtacaaattc attaagcaaa taattgacag gtacggtaat aactaacaca aaagtaactg
     2701 caatacctaa acctgcagca gtgctcacat ttttggaaac agcaagatac gaacacatac
     2761 ccagaaagta tgcaaatacc atgttgtcta taaaaattga ccgaacgaat atatctaata
     2821 aatgttccat actttcttat tttataagtt ctttatttcg tgaacgttga atccaaatta
     2881 taataccaac aacaaataat gccataggtg ataataacat aagtccattg tttacatatc
     2941 caaaatcgta taaaccatga ggaattacat gcaactcacc tatgccgggc aatgtaactg
     3001 tacccgagcc aaaaatttct ctcacaacag aaactaacac taatataata ccataaccta
     3061 acccgttacc aataccatcg agtaacgaag gccatggttt attaccaaga gcaaacgctt
     3121 ctaaacgtcc cattaaaata cagtttgtaa taataagccc aacaaatacc gataattgtt
     3181 tacttatatc atatgcataa gctttcaaaa tctggtcaac tataattaca agtgcagcta
     3241 ttactattaa ttgcacaata attcgaattc tgtcgggtat agttttacgc aacaatgaaa
     3301 taataacatt ggcaaatgct aacactatag taaccgacaa tgacattact atagcaggtt
     3361 ctacttttac agtaactgct aaagcagaac atattcccaa cgattgaaca gtaataggat
     3421 tttcggcacc taaaggtcct gtaagcaatc gtatattttt tttcgaaaac aatggttctt
     3481 tatctgcact cattttttta ttgtttaata gattgtaaat atgaaacata ggtgctcaaa
     3541 caggtatcga gcatagcatt tacaccatta ctggtaatag taccacccga aattgcatct
     3601 acaccatgaa tattatcggg agcagcacct ccttttataa ctttaataga aataaatgtt
     3661 cccgaagtat caaaaatttg ttttcctcta aacatgtgtt caaatttatc tttattgatt
     3721 tcggctccta atccgggtgt ttctccttga tgatcgaaca ctgcgccata cacagtatta
     3781 ttatcgcttt caaacgatat aaatccccaa attggtcccc ataacccttt accacgcata
     3841 ggaataataa tatatttttt tccgtctttt tgtgcttcaa ataagggtaa ggtacgttga
     3901 gcacgaggca ttgcatacaa ttttttcata tctatatcaa aagcccgtat cgtatcggtt
     3961 aatttaccat taggaacaag tacataagga attacgttac cgtttatatc aataattgct
     4021 gaatttacaa tatactttgc atatagttct tcaacattat ctgtagtaga ttctacttga
     4081 atcgatgata agatattttg cattttctct tttcgaacat ttgacttttg tgtatcttta
     4141 agagaatatg aagcaataga taataacgca gctactaata ctaccattat taccgaatat
     4201 gtaaatatat atgaattact tctttccata gtattattta tttagctgtg aaacttttgc
     4261 tctgcgaagc cgctttttaa tatttgactg aataaccaca tggtctataa gcggcgagaa
     4321 cacattcata aacaatattg ccaacatcat accttcggga tatgcaggat ttaaacaacg
     4381 aataataata gcaaatgctc ctattaaaaa tccataaatc catttaccct tgcccgtttg
     4441 tgctgcagtt accgggtcgg tagccataaa tacagcacca aacgcaaatc cacctataaa
     4501 catgtgttcc aatgcaggag tattcataag tatatttccg gcaggtgcta ccgcattaag
     4561 aagggatgcc attaaaaatc caccggcaaa aacagaaaac ataattctaa aacttgctat
     4621 acctgtatac aacaaaatta tagcacctat agcaatagct attacagacg tttcgccaac
     4681 cgaaccggca tagttaccta ccaataaatc aaatgtagac aattgattat ccacaaatcc
     4741 gctatatgc
//
LOCUS       DAOTOW010000076         4745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14804_length_4745_cov_3.816418, whole genome shotgun sequence.
ACCESSION   DAOTOW010000076 DAOTOW010000000
VERSION     DAOTOW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14804_length_4745_cov_3.816418"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..624)
                     /gene="tpx"
                     /locus_tag="P5243_01990"
     CDS             complement(124..624)
                     /gene="tpx"
                     /locus_tag="P5243_01990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRS18248.1"
                     /translation="MATITLKGNEIQTIGSLPAIGSQAPNVQLVTTDLQTVSISDYKG
                     KKVVLNIFPSIDTGVCAASVRAFNSHANSLPNTVVLCISRDLPFAQARFCGAEGLTNV
                     INLSDFNTGDFGKSYGLEITSGPLKGLHSRAVIILDEQGTVKYTEQVPEIAQEPNYNA
                     ALSALQ"
     gene            complement(808..1557)
                     /gene="truA"
                     /locus_tag="P5243_01995"
     CDS             complement(808..1557)
                     /gene="truA"
                     /locus_tag="P5243_01995"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRS18249.1"
                     /translation="MRYCIHLAYNGTRFHGWQTQPNAISVQACIEECLSKIIGESIEI
                     IGAGRTDTGVHASNYYAHVDITHEINCTDVQYKLNKMLSHDIVIYTIFKVNNEFHARF
                     SALWRTYTYTLSLQKNPFLQETTWLYSYPLNIEAMNNAAALLQTYTDFTSFSKLHTDV
                     NNNNCTITYAKWEQKGDLLIFTITANRFLRNMVRAITGTLIDVGRGKTSIPEFCTIIE
                     SKNRQNAGTSIPALGLCLVDIGYEWEHYKCK"
     gene            1705..>3332
                     /locus_tag="P5243_02000"
     CDS             1705..>3332
                     /locus_tag="P5243_02000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS18250.1"
                     /translation="MESRFAVIIQKHRLIGWVLAAFDIVKNADKDFYEPVEYLLPESN
                     TKYTASYEKKIVSLIAKYDEKQLFTKFAKKKKDAQKDFLEKVKEDYFTEEIRPYIEKH
                     IYECFKIIKDHAVPLYLREGEANLYSEDLITYPTNAPQVTFNFNKTQTGLEYGLDVVV
                     NSKPISLIGKPFVVLSNSPGLIIIEKTLYHLQTIDSKKLLPFFNKKQIVIPQKFEQQY
                     FDTFVLNCIKNFTVTNKGFDIIDIYEKPTLTASIVRDIQNNAAIEFGISYRKWKLNDF
                     GITQPYSVEYTNIKNVPKYVRVHRNFEFEKEFASILLQENLIHEGNLWKVKGYNSDGY
                     YDILQWIREHKQIIDSYAIEIFDESDKKIQNLQAHIDISVSSDSIDWFDVHAIVSFGE
                     YKIPFKRLRRNILQEDPVVILPDNQIGIIPTEWFARFKDLFLFSSKNQDPEVFTLKTI
                     HYKTIQRLPVDFSDALKTRFLNIDTNGIRDNEVPAEIQATLRPYQIEGYRWLCYLDAN
                     NFGGCLADDMGLGKTLQTITLLQRVINVQKESNQ"
     assembly_gap    3333..3342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3343..>4347
                     /locus_tag="P5243_02005"
     CDS             <3343..>4347
                     /locus_tag="P5243_02005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS18251.1"
                     /translation="VVAPASIVHNWYNEFKKFAPGIKIFKYEGNDRNKEVMYFKKYHV
                     VITTYGLLRNDIESFERFPFYYIILDESQMIKNPDSKIYSAVLRLQSQRKLVLTGTPI
                     ENSLIDLWAQLNFVNRDMLGSLAFFKEHFIKGTEKQNELIENQLKKIVKPFIFRREKQ
                     EVAKDLPPLTEQVRICKMSDEQEKIYETEKSKVRNMIFETIEKESFQKSTINVLQALM
                     KLRQIANHPQLIEGLSGDSGKFDEVIRVLQNLIHNHKVLIFSSFVKHLDLFKDYFASQ
                     GISYAYLTGSTKNREQVIQEFQDNDECKIFLISIKAGGVGLNLTKADYVFILDPWWNP
                     A"
     assembly_gap    4348..4357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4358..4553
                     /locus_tag="P5243_02010"
     CDS             <4358..4553
                     /locus_tag="P5243_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18252.1"
                     /translation="NQAISRAHRIGQKNNVNVYRFISENTIEEKIQRLQRKKSELAKN
                     YIYEEHSIPFTQEEVSYLVE"
BASE COUNT         1709 a          645 c          759 g         1612 t
ORIGIN      
        1 acctcgtagt tattctgtag atgctataaa agatacaatt tattcaaaag cacaaataat
       61 atttgcaaat caaaattata agggtattca atggtatgct aatgttgatc cgtattaatt
      121 tttttattgt aatgctgaaa gtgctgcatt ataattaggt tcttgtgcaa tttcaggtac
      181 ttgttctgta tattttacag tgccttgttc atcaagaatt attactgctc gcgaatgaag
      241 accttttaat ggaccacttg ttatttctaa tccatatgat ttgccaaaat cacccgtgtt
      301 gaaatcagaa agatttataa catttgttaa cccttcggca ccacaaaatc gagcttgggc
      361 aaaaggtaaa tcacgtgaaa tacacaatac cacagtatta ggtaaagagt ttgcatgtga
      421 gttaaatgct cttacagatg cagcacatac tcctgtatca atacttggaa aaatatttaa
      481 tacaactttt tttcctttat aatctgaaat tgaaactgtt tgtaagtcgg tagtaaccaa
      541 ctgtacgtta ggagcttgtg aacctattgc agggagtgac ccaattgttt gtatttcgtt
      601 tcctttcaat gtaattgttg ccatgagtta tatgttttat aagttactta ttttatagaa
      661 aatacaccta aatattaggt atttgcttta ctagtatgac ttttagattg taaagacctt
      721 acacgttatt tgcaaaaaat tatttgtatt atacttaaac aaaagaccta ttatatattt
      781 ttttgaaata aaagagttgt tttataatta cttacattta taatgttccc attcataacc
      841 tatatcaaca aggcataaac caagtgcagg gatagatgtt cctgcatttt gtctgttttt
      901 actttcaata atggtacaaa attcaggaat acttgtttta cctctgccaa catctattaa
      961 agttcccgta attgctcgta ccatatttcg caaaaaacgg tttgcggtaa ttgtaaaaat
     1021 taacaagtct cctttttgtt cccatttagc ataagtaatt gtacaattat tgttgtttac
     1081 atcagtatgt aatttactaa aacttgtaaa gtcggtatat gtttgcaaaa gtgccgcagc
     1141 attattcatt gcttctatat tgagcggata tgaatacaac catgtagttt cttgcaaaaa
     1201 tggatttttt tgcagactga gtgtgtaggt atatgtgcgc catagagccg aaaatcgagc
     1261 gtgaaattcg ttattgactt taaaaatggt ataaataact atatcatgcg aaagcatttt
     1321 attgagtttg tattgtacat cggtacagtt tatttcatga gtaatatcaa catgagcata
     1381 ataattagaa gcatgcacac cggtatctgt tctgcctgct ccaattattt caatagattc
     1441 accaataatt ttgcttaaac attcttcaat acatgcttgc acagaaatgg catttggttg
     1501 tgtttgccaa ccatgaaatc gagttccgtt gtatgctaaa tgtatacagt accgcataat
     1561 ttgtatgagt aatttgtaat tgcatacaaa tgtacataaa agcaataaaa accgagaaca
     1621 aatgttatag tgttaaaaaa aaataaaaaa aaaatactta aaacttcaaa tcttgtggta
     1681 ttttcgttga attaatttca aaacatggaa tctcgttttg ctgtaattat tcaaaaacac
     1741 agacttatag gctgggtgtt ggcagcattt gatattgtta aaaatgccga caaagacttt
     1801 tatgagcctg tagaatattt attgcccgaa tcaaatacaa aatatacagc atcgtacgaa
     1861 aaaaaaattg tttcattaat tgctaaatat gatgaaaaac aattatttac aaaatttgcg
     1921 aagaaaaaaa aagatgcaca aaaagatttt ttagagaaag taaaagaaga ttattttacc
     1981 gaagagattc gtccctatat tgaaaaacat atatatgaat gttttaaaat tattaaagac
     2041 catgcagtgc ctttatatct tcgtgaaggt gaagcaaatt tatattcgga agatttaatt
     2101 acatatccta caaatgcacc gcaagtaaca tttaatttta ataaaacaca aacaggattg
     2161 gagtacggat tagatgtagt tgttaattct aaacctattt ctttaatagg taaacctttt
     2221 gttgtattat ctaattctcc gggacttata attattgaaa aaacattgta tcatttacaa
     2281 acaattgata gtaagaaact tttacctttt tttaataaaa agcagatagt tataccgcaa
     2341 aaatttgaac aacagtattt tgacactttt gtactaaact gtattaaaaa ttttacggtt
     2401 acaaataaag gatttgatat aattgatatt tatgaaaaac caacacttac cgcaagtata
     2461 gttcgtgata ttcaaaacaa tgctgctata gaatttggta tttcttatag aaagtggaaa
     2521 cttaatgatt ttggtattac acaaccgtat tctgtagaat ataccaatat aaaaaatgtt
     2581 cctaaatatg tacgagttca tcgtaatttt gaatttgaaa aagaatttgc atcaatactt
     2641 ttacaagaaa atttgataca tgaaggaaat ttatggaagg ttaaagggta taattcagat
     2701 ggatattatg atattttaca atggatacgt gaacataagc aaataataga ctcttatgct
     2761 attgaaatat ttgatgagtc tgataagaaa atacaaaatt tacaagcaca cattgatatt
     2821 tcggtatctt cagattctat tgattggttt gatgttcatg caatagtgtc atttggtgaa
     2881 tataaaatac ccttcaaacg gttacgtcga aacattttgc aagaagatcc ggtagtaatt
     2941 ttgcctgata atcaaatagg tataattcca acagaatggt ttgcacgatt taaggattta
     3001 tttttattca gttctaaaaa tcaagaccct gaagttttta ctcttaaaac aattcattat
     3061 aaaacaattc agcgtttgcc ggttgatttt agcgatgcct tgaaaacacg atttttgaat
     3121 atagatacta atggtattcg tgacaatgaa gtgcctgctg aaattcaagc tactttaaga
     3181 ccttatcaaa tagaaggata tcggtggtta tgttatcttg atgctaataa ttttggtggt
     3241 tgtttggctg acgatatggg tttaggtaag acattacaaa caattacatt gttacagcga
     3301 gtaattaatg ttcaaaaaga atcaaatcaa aannnnnnnn nnagtagttg ctcctgcatc
     3361 tattgttcac aattggtata atgaatttaa aaagtttgct ccaggtatta aaatatttaa
     3421 atatgaagga aacgatagaa acaaagaagt aatgtatttt aagaaatatc atgttgtaat
     3481 tacaacctat ggtttgttgc gaaatgatat tgaaagcttt gaaaggtttc cgttttatta
     3541 tattatactt gacgaaagtc aaatgataaa aaatccggat tcaaaaattt atagtgcggt
     3601 tttgcgttta caatctcaac gtaaattagt acttaccggt actccaattg aaaattcttt
     3661 aattgattta tgggcccaac ttaattttgt gaatcgtgat atgcttggta gtttggcttt
     3721 ttttaaagag cattttataa aaggtaccga aaaacaaaat gaattaatag aaaatcaatt
     3781 aaaaaaaatt gtaaaaccat ttattttccg tcgcgaaaaa caagaagtag caaaagattt
     3841 accaccacta accgaacagg ttcgtatatg taaaatgagt gatgagcaag aaaaaatcta
     3901 tgagaccgaa aaatcaaaag ttcgtaatat gatttttgaa actattgaaa aagaatcgtt
     3961 tcaaaagtca actataaatg tattgcaagc tcttatgaag ctacgccaaa tagcaaatca
     4021 cccacaatta attgaagggt tatcaggtga ttcaggtaaa tttgatgagg tgatacgggt
     4081 attgcaaaat ttaatacata atcataaagt tcttatattt tcttcgtttg taaagcattt
     4141 agatttattt aaagattatt ttgcttctca aggaatatca tatgcatatc tcaccggtag
     4201 tactaaaaat agagaacaag taattcagga atttcaagat aatgatgagt gtaaaatctt
     4261 tttaatttct ataaaagccg gtggagtagg tttgaattta actaaggcag attatgtatt
     4321 tatattagac ccttggtgga atcctgcnnn nnnnnnnaaa tcaggcaatt agtagagctc
     4381 atcgtatagg gcaaaaaaat aatgtgaatg tttataggtt tatttctgaa aatacaattg
     4441 aagaaaaaat tcaacgattg caacgaaaaa aatcagaatt ggctaaaaat tatatatacg
     4501 aagaacactc tatcccattt acacaagaag aagtttctta tttagtggaa tagagtgaaa
     4561 agtgcagtat cgttttataa atattataat ttaccacttt ctgcaaaatc aattcgaatt
     4621 aatgggaatc gaattttaat agcatctatt tgtgcaagag gcaccgaaga atcaacacgc
     4681 aaactggtaa ttttatttaa actgtataaa gcttttatat cagttacttt tgagttgtaa
     4741 acaat
//
LOCUS       DAOTOW010000077         4698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_14996_length_4698_cov_3.293129, whole genome shotgun sequence.
ACCESSION   DAOTOW010000077 DAOTOW010000000
VERSION     DAOTOW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14996_length_4698_cov_3.293129"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="P5243_02015"
     CDS             complement(<1..200)
                     /locus_tag="P5243_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18253.1"
                     /translation="MFRYHKHISIQTKNVLLGILLCFAASCSITKKIPEQSQLLTKTK
                     IVCNEKSIDTDDIKNYLKQKPNR"
     gene            259..1032
                     /locus_tag="P5243_02020"
     CDS             259..1032
                     /locus_tag="P5243_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRS18254.1"
                     /translation="MLSKNKLKFFSSFSSKKIREEYGLFVVEGNKMIDELLVSNHTIH
                     SIVATSDWIEKHTKLVANVEVLEASSKDLECISLLKSPQDAWALVYTEYKSDPLLIEQ
                     SELVLALDTIQDPGNLGTIIRLADWFGVRQIWCSSQTVDVYNPKVVQASMGSIFRVHM
                     VYGNLFTLLAQHSYIPIYAADLQGENVYTQQLPKQAIVVMGNEGNGISQEILPFISTF
                     LHIPSFSTHSHKPESLNVSVATAILCSEFKRIGQFINKI"
     gene            1158..2261
                     /locus_tag="P5243_02025"
     CDS             1158..2261
                     /locus_tag="P5243_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18255.1"
                     /translation="MKKVLLSISVAVLFCFGAIAQTIEMPWNQYGGGDPVIAYQAKDA
                     SKVTTYTPQVGDVVTVTIAGTANYDISQFQVAVVDDAPSSYWKELAGFKSLGNVTAGT
                     PFSFTVDLTITQTGSPKIVFDGKNATLAGTNGAGTSITLSLTSYTVSIFTPIAGAKIL
                     TDNGDGTKQEKFTDVLALDPAVKVGDVVRVTLKGTADVSATSFQTVIVDGTAAASYWK
                     ELSAWTAFTPAAVTANQQFTLVADIEITSAPTGTGAGSQNVVLSAVSSANLIQLSLTE
                     FSAVIVDPNDDDVDPGTGIQDLSSTSIQPKVVNGELTVGTDATLIIVTSIDGKVLKYS
                     ASKSVNVSDLPAGTYVASVTLPSGVVVSKAFVK"
     gene            2366..>4698
                     /gene="leuS"
                     /locus_tag="P5243_02030"
     CDS             2366..>4698
                     /gene="leuS"
                     /locus_tag="P5243_02030"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRS18256.1"
                     /translation="MEYNFTEIEKKWQDYWEANKTFKTVLDTSKPKFYALDMFPYPSG
                     AGLHVGHPEGYTASDIVSRYKRMNGFNVLHPMGWDAFGLPAEQYAIQTGTHPAITTQK
                     NCDTFRRQIKALGLSYDWDREINTTDPKYFKWTQWIFIQLYNTYFDTTIQKGRPISEL
                     PLPAEVSALGKQAVTKYINDKRLAYYDNAQVWWCSHCKTVCANEEVLNDGSHEKCGNQ
                     VSKKNLKQWMLRIPHYAERLLQGLDTVDWPEGVKDMQRNWIGRSTGAEVVFALDGLQE
                     TLTVYTTRPDTLFGATYMVIAPEHPMVDTIVTSDKKNEVDAYIKQAALKSDLDRTELA
                     KDKTGVFSGRYAINPLTGTKIPIWIADYVLMGYGTGAIMAVPAHDTRDFEFAQKFNIP
                     IICILDPQSADAQTRELVLAGKACWTEDGAYINSANHALGIDINGKNKKEGIATIISW
                     LESKNIGKATVNYKIRDWLFSRQRYWGEPFPIIHWEDGEISTIEVDKLPHNLPVLEKY
                     EPGDDAESPLANAKDWLYVTDANGRKGRYETNTMPQWAGSCWYYLRYIDPTNDAEPFS
                     KVAENYWMPVDLYIGGAEHAVLHLLYSRFWHKVLFDLGIVSTDEPYQKLYNQGMILAF
                     AYETKTGAKIPSDKVEERDGGFYHIETGEELKQIVAKMSKSLKNVVNPDDVIAKYGAD
                     SLRLYEMFMGPLDATKPWADNGVKGVFNFLARVYRFFAEPANVEKGVTDDTMVLKELH
                     KTIKKVADDIENLRFNTAISQMMVFTNTCYKAGKVST"
BASE COUNT         1568 a          732 c          921 g         1477 t
ORIGIN      
        1 cgattaggtt tttgtttgag ataatttttt atatcatcag tatcaattga tttttcatta
       61 caaactattt ttgttttagt aagcaattgt gattgttcgg ggattttttt tgtaatagag
      121 caacttgcgg caaaacaaag taatatgcct aataatacat ttttagtttg aatactgata
      181 tgtttgtgat atctaaacaa tttcgctatt tttgacaaaa ataattcttt aattttgatt
      241 tcatagccta ctcaaactat gttaagtaaa aataaattaa aattttttag ttctttttct
      301 tctaaaaaga ttcgtgagga atacggcttg tttgtagtag agggaaacaa aatgatagat
      361 gaattacttg tttcaaacca tacaattcat agtatagttg ctacatcaga ttggatagaa
      421 aagcatacaa aattggttgc aaatgttgaa gtattagaag caagttctaa agatttggaa
      481 tgcatttcat tgcttaaatc accacaagat gcttgggctt tggtttatac ggaatataaa
      541 agtgacccat tgttgataga acagtctgag ctcgtattgg ctttagacac tattcaagac
      601 ccgggtaatt taggaacaat tattcgattg gccgattggt ttggtgttcg tcaaatatgg
      661 tgtagctctc aaacggttga tgtttataat cccaaagttg ttcaggcaag tatgggttca
      721 atttttcgtg tacatatggt ttatggtaat ttgtttacac tattggctca acattcttat
      781 attcctatat atgctgccga tttacaaggg gaaaatgtat atacacagca attgcctaaa
      841 caagcaattg tagtaatggg caacgagggg aatggtatat cgcaagagat tttaccattt
      901 atttcaacat ttttacacat accttctttt tcaacacata gtcataaacc agaatcattg
      961 aacgtatcag tagctacggc aatattgtgt tctgagttta agcgtatagg acaatttatt
     1021 aacaaaatat aaaaacatat attttggatt gttattttta ttattattgc aaataatgtg
     1081 taaaagaaat atttggtaaa gagttcccaa atatttttta tggagatatt gaacttatta
     1141 atttaaaatt tttaacaatg aaaaaagtat tactatcaat ttcggtagca gtattattct
     1201 gctttggagc tattgctcaa actattgaaa tgccttggaa tcagtatgga ggaggcgatc
     1261 ctgtaattgc gtatcaagct aaagatgcat ctaaagtaac aacctatact ccacaagtag
     1321 gtgatgtagt taccgttact attgcaggta cagcaaatta tgacatcagt caatttcaag
     1381 ttgcagtggt tgatgatgca ccttcatcat attggaaaga attagcaggt tttaaatcat
     1441 taggtaatgt aacagccggt acaccgttta gttttactgt tgatttaact attacgcaaa
     1501 caggttcgcc taaaatagtt tttgacggta aaaatgctac attagctgga acaaatggcg
     1561 caggaacaag tattacatta agtttaactt catatacagt ttctatattt acaccaattg
     1621 caggtgcaaa aatattaaca gataatggag atggaacaaa acaagaaaaa ttcactgatg
     1681 ttttagcatt agatcctgct gtaaaggttg gcgatgtagt acgtgtaaca ttaaaaggaa
     1741 ctgctgatgt tagtgctact tcattccaaa cagttatagt tgatggtact gcagctgcaa
     1801 gttactggaa agaattaagt gcttggactg catttacacc tgctgctgtt acagcaaatc
     1861 aacaatttac attggttgca gatattgaaa ttactagtgc acctaccgga acaggagccg
     1921 gttcacaaaa tgtagtatta agtgctgtgt caagtgccaa tttaattcaa ttatccttaa
     1981 ctgaattctc tgctgttatt gttgatccaa atgatgatga tgtagatccg ggaacaggta
     2041 ttcaagattt gtcttcaact tctattcaac ctaaagtagt aaatggtgaa ttaacagttg
     2101 gaacagatgc tacacttatc attgttactt caattgatgg aaaagtacta aaatacagtg
     2161 catctaaaag tgtaaatgtg tctgatttac ctgccggaac atatgtggct tcggttacat
     2221 taccaagtgg tgtagttgtt tcaaaagcgt ttgtaaaata agtattatat aacatacatt
     2281 aattcaagag ggtgtttctt atgaaacacc ctttttttta taaacttttt tgtattttcg
     2341 cacctcaatt tttacaattc atacaatgga atacaatttt accgaaatag aaaaaaaatg
     2401 gcaagactac tgggaagcca ataaaacgtt caaaactgta ttagatacat caaaaccaaa
     2461 attttatgcg ctcgatatgt ttccgtatcc atcgggtgcc ggattacacg taggccaccc
     2521 cgaaggctat accgcttcgg atattgtaag tcgttacaaa cgtatgaatg ggttcaatgt
     2581 gcttcatcct atgggctggg atgcatttgg gttgcctgcc gaacaatatg caattcaaac
     2641 aggtactcac cccgctatta ctactcaaaa aaattgcgat acttttcgtc gtcagattaa
     2701 agcacttgga cttagctatg attgggatag agaaataaat actacagacc caaaatattt
     2761 taaatggaca cagtggattt ttattcaact ttacaatact tattttgata caacaattca
     2821 aaaaggacga ccaatttcag aattaccttt accagcagaa gtttctgcat taggaaaaca
     2881 agcggtaaca aaatatataa acgacaagcg tcttgcttac tacgataatg cacaagtatg
     2941 gtggtgcagt cattgtaaaa ccgtgtgtgc caacgaagag gtgttgaatg atggaagtca
     3001 tgaaaaatgc ggcaatcagg tatcaaagaa aaatttaaaa caatggatgt tgcgaattcc
     3061 acactatgcc gagcgattgc ttcaaggtct tgatactgtt gattggcccg aaggtgtgaa
     3121 agatatgcaa cgaaactgga taggcagaag caccggtgct gaggttgttt ttgctcttga
     3181 tggattacaa gaaacgctta cggtttatac tactcgtccc gatacattgt ttggtgcaac
     3241 ctatatggtt attgctcccg aacatcctat ggtagatact atagtgacaa gtgataaaaa
     3301 aaatgaagta gatgcctata ttaaacaagc tgctcttaag tctgacttag atagaactga
     3361 acttgcaaaa gataaaacag gagtattttc cggtcgatat gcaataaacc cacttaccgg
     3421 aaccaaaatt ccgatatgga ttgccgatta tgtgcttatg ggatatggta ccggtgctat
     3481 tatggcggtg cctgctcatg atactcgcga ttttgaattt gctcaaaaat tcaatattcc
     3541 tattatttgt atacttgacc cacaaagtgc cgatgctcaa actcgtgaac ttgtattggc
     3601 aggtaaggct tgttggacag aagatggggc atatataaat tctgcaaatc atgcattggg
     3661 gattgatatt aacggaaaaa ataaaaaaga aggaatagca actattattt cgtggttaga
     3721 atcaaaaaat attggaaaag ctacagtaaa ttataaaatt cgtgattggt tgttcagccg
     3781 tcaacgatac tggggtgagc cttttccaat aattcattgg gaagatggag aaatttctac
     3841 cattgaagtt gataaattac cgcataattt acctgtactt gaaaaatatg agcccggtga
     3901 tgatgctgaa tcgccacttg caaatgctaa ggattggtta tacgtaaccg atgccaatgg
     3961 gcgaaaaggc agatacgaaa ctaatacaat gccacagtgg gcaggttctt gttggtatta
     4021 tttacgatat attgatccaa ccaacgatgc cgagccgttt tcgaaggtcg cagaaaatta
     4081 ttggatgccc gtagatttat atattggtgg tgccgagcat gcagtattgc acttgttata
     4141 tagccgtttt tggcataagg ttttgttcga tttaggaatt gtttcaacag atgaaccata
     4201 tcaaaaatta tataatcaag gaatgatatt agcatttgct tatgaaacta aaacgggagc
     4261 aaaaatacca tcggataaag tggaagaacg tgatggcgga ttttatcaca ttgaaacagg
     4321 cgaagaatta aaacaaatag ttgcaaaaat gtcgaaatca cttaaaaacg tagtgaatcc
     4381 agacgatgta atagcaaaat acggtgccga tagtctccgc ttgtacgaaa tgtttatggg
     4441 gccccttgat gctactaaac cttgggctga caatggagta aaaggtgtat ttaatttctt
     4501 ggctcgagta tatagattct ttgcagaacc tgcaaatgta gaaaaaggtg ttaccgatga
     4561 tactatggta ctgaaagaat tgcataaaac aataaaaaaa gttgctgatg atattgaaaa
     4621 tttgagattt aatacggcaa tttcacaaat gatggtgttt accaatacgt gctataaagc
     4681 aggaaaagta agtacaga
//
LOCUS       DAOTOW010000078         4671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15109_length_4671_cov_3.282929, whole genome shotgun sequence.
ACCESSION   DAOTOW010000078 DAOTOW010000000
VERSION     DAOTOW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15109_length_4671_cov_3.282929"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="P5243_02035"
     CDS             complement(<1..390)
                     /locus_tag="P5243_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18257.1"
                     /translation="MKKIVSIGILSLFLCNFGFSQTDEFVLTWPIVIAEAKKADAEVE
                     NPKKAANIKSWTERGRKYLEVFTFDLKAAHPGMPEDQILLYMKSPAKKTWQEGAYNVY
                     SFDRIDLYFKNKALAKYERTGNDVAQYF"
     gene            complement(437..2974)
                     /gene="gyrA"
                     /locus_tag="P5243_02040"
     CDS             complement(437..2974)
                     /gene="gyrA"
                     /locus_tag="P5243_02040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRS18258.1"
                     /translation="MTEGEKIIQLNIEEEMKSAYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLFGMHELGVRSNTAYKKSARIVGEVLGKFHPHGDSSVYFTMVRMAQDWSLRYPLV
                     DGQGNYGSVDGDPPAAMRYTEARLQKIAEETLIDIDKETVDFQLNFDDTLEEPTVLPT
                     RIPNLLVNGTSGIAVGMATNMAPHNLSDTIDAIVAYIDDKEVDIQRLIEIIKAPDFPT
                     GGIIYGYQGVKEAYETGQGRVVIRGKAFIESENGKEKIIVSEIPYMVNKAEMIKKTAD
                     LIIDKKIEGISEVNDESDKDGMRIVYTLKRDAIANVELNKLYKYTGLQTSFSVNNIAL
                     VDGRPRLLNLKDLIKYFVKHRHDVVIRRTKFELRKAQERAHILEGLIIASDNIDEVIA
                     IIKQSETQNAAKATLMERFNLSDIQTNAIIQMRLGQLTQLDQNKLRNEYNDIIALIKH
                     LEEILASEALQMQIIKDELIEIKNKYGDARKTEIVHASEDFNPEDFYADEDVVISISH
                     MGYIKRTPLSEYKIQNRGGVGARGADTREEDFIEHLYVATMHNTMLFFTEKGKCFWLK
                     VYEIPEGSKTSKGRAIQNMLNIEPGDKVKAYINVTSLTDEEYINHNYIILATAKGIIK
                     KTSLEAYSRPRQNGINAVTIREGDQLLEACMTNGNHDILMAVKSGKAIRFPESKIRPM
                     GRTAAGVKGISISDKDEVVGMICVPPDVKKEILVVSEHGYGKRSAIEDYRITNRGGKG
                     VKTINITSKTGSLVAIKAVDDNDDLMIINKSGITIRLEVATLRVMGRAAQGVKLINLK
                     NNDSIAAITAIDVSEDERMQRELERSGETNSEDDIENNSEISENEQE"
     gene            3221..>4671
                     /locus_tag="P5243_02045"
     CDS             3221..>4671
                     /locus_tag="P5243_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRS18259.1"
                     /translation="MDKEFSEHMKEVLSYSREEAIRLGNSMIDADHLFLGLLREGTSQ
                     AVRILEALHCNIEYTKQQIDNSLREPMPLSEDEQEKIPLKRTADKILKISILESKFLN
                     HSKVEPEHLLLALLKENNSLSAEIMNKQDITYEKVKQHIQIPNPENKSQNPFGDDDDD
                     MSKSQKQPNSPKTEKPSETPVLDNFGVDLTKAAEEGRLDLVVGREKEIERIAQILSRR
                     KKNNPILIGEPGVGKSAIAEGLALRIVQKQVSRILFNKRVLTLDLASIVAGTKYRGQF
                     EERMKAMLNELARSKDVILFIDEIHTIVGAGGASGSLDAANMLKPALARGEIQCIGAT
                     TLDEYRQHIEKDGALERRFQKVMVEPATVDEAINILNNIKKHYEKHHGVYYTDDAIKA
                     CVYLTHRYITDRHLPDKAIDALDEAGSRVHIQNIQVPTTIVSLEKEIEELKQKKIDAV
                     RNQNFELAASYRDNEKMLIKQIEEENKKWQDMLL"
BASE COUNT         1536 a          816 c          676 g         1643 t
ORIGIN      
        1 aaaatattga gcgacatcat tacctgttct ttcatattta gctaaggctt tatttttaaa
       61 atacaaatca attctatcaa atgaatatac attgtatgct ccttcttgcc atgttttttt
      121 tgcaggtgat ttcatataca ataatatttg atcttcgggc attcccggat gagcagcttt
      181 taaatcaaat gtaaatactt ctaaatattt acgtccgcgt tctgtccatg attttatatt
      241 tgctgctttt ttaggatttt caacttcggc atcagctttt tttgcttctg caatcactat
      301 aggccatgtt aatacaaatt catcggtttg gctaaatccg aaattacata agaataatga
      361 tagaattcct atacttacaa tttttttcat attcatttta ttttttaatt attttgaaca
      421 aaaataataa aaattattat tcttgttcat tttcagatat ttcagaatta ttttcgatat
      481 catcttcact gtttgtttca ccactgcgtt ctaattctcg ctgcatacgt tcatcttcac
      541 tcacgtcaat agctgtaata gctgcaattg aatcattatt tttgaggtta attaatttta
      601 ccccttgagc agctctaccc attacgcgta acgttgcaac ttctaaacgt attgtgattc
      661 ctgatttatt tataatcatt aaatcatcat tatcatcaac tgctttaata gcaacaagag
      721 aaccggtttt tgaagttata ttaatagttt taactccttt accccctctg tttgtgattc
      781 tgtagtcttc aatagccgaa cgttttccat aaccatgttc agatactaca agaatttctt
      841 tttttacatc aggtggcaca caaatcattc ctaccacttc gtctttatct gatattgaaa
      901 ttccttttac tcccgctgct gttctaccca ttggtctgat tttagattct gggaatctaa
      961 ttgctttacc tgattttaca gccattaaaa tatcgtgatt accatttgtc atacatgctt
     1021 ccaacaattg atcaccttca cgaattgtaa cagcattgat accattttga cgaggacgag
     1081 aatatgcctc aagtgatgtt ttcttaataa taccttttgc tgttgctaat attatataat
     1141 tatgattaat atactcttca tctgttaaag atgtcacatt tatatatgct tttactttat
     1201 cccccggttc tatattcaac atattttgga ttgctcttcc ttttgatgtt ttagaacctt
     1261 cgggaatttc atatactttg agccaaaagc atttaccttt ttcagtaaag aacaacatag
     1321 tattatgcat tgttgcaaca tacaaatgct caataaagtc ttcttcgcgt gtgtcagccc
     1381 cgcgagcacc tactcctcct ctattttgaa ttttatattc actaagtgga gtacgtttaa
     1441 tgtagcccat atgtgaaatg ctaattacaa catcttcatc tgcatagaaa tcttcaggat
     1501 taaaatcttc agaagcatgt actatttcag tcttacgagc atcaccatat ttgtttttaa
     1561 tttcaattaa ttcatcttta ataatttgca tttgtaatgc ctcacttgcc aaaatttctt
     1621 caagatgttt tattaatgca attatatcgt tgtattcgtt acgtaattta ttttgatcga
     1681 gctgtgttaa ctgccctaaa cgcatttgaa taatagcatt agtttgaata tcgcttaaat
     1741 tgaagcgttc cattaatgtt gccttagctg cattttgtgt ttctgattgc ttaattatag
     1801 ctattacctc gtcaatattg tcacttgcta ttattaatcc ttcaagaata tgagcacgtt
     1861 cttgagcttt tcgtaattca aattttgttc ggcgaattac aacatcatgt cgatgtttta
     1921 cgaaatattt tattaaatct ttcaaattca ataaacgcgg acgaccatca accaatgcaa
     1981 tattatttac actaaacgat gtttgaagtc ctgtatattt atacaatttg tttaattcaa
     2041 cattcgcaat tgcgtcgcgt ttaagggtat acactattcg cataccgtct ttgtctgatt
     2101 catcatttac ttcagaaata ccttcaattt ttttatctat aattaaatca gctgtttttt
     2161 taatcatttc agctttatta accatatagg gtatttcact tactataatt ttttcctttc
     2221 cgttttcact ttctataaag gctttacctc ttataacaac acgcccctgc cctgtttcat
     2281 aagcttcttt aactccttga tatccatata taatacctcc tgtaggaaaa tcaggtgctt
     2341 taattatttc tattaaacgt tgtatatcaa cttctttatc atctatatat gcaacaatcg
     2401 catctatagt atcagataaa ttgtgagggg ccatattggt tgccataccc acagcaattc
     2461 ccgatgttcc gtttactaat aaattaggta ttcgtgttgg taaaactgta ggttcctcta
     2521 atgtgtcatc aaagtttaat tgaaaatcta cagtttcttt atcaatatca atgagtgttt
     2581 cttctgcaat tttttgcaaa cgagcctctg tgtatcgcat agcagccggt gggtcaccat
     2641 caacagaacc atagttacct tgcccgtcta ctaaaggata acgcaatgac caatcttgtg
     2701 ccattctaac catagtaaaa tatactgaag aatccccatg tgggtgaaat ttacctaaaa
     2761 cctcccctac aattcttgct gattttttat atgcggtatt tgagcgaaca cctaattcat
     2821 gcataccaaa aagtactctt cggtgaacag gtttaaaccc gtcgcgaaca tcaggtaatg
     2881 ctctagaaac tattacggac attgaataat caatgtatgc cgatttcatt tcttcttcaa
     2941 tatttaattg aataattttt tctccctcag tcatagttta tatatgattt ttactataat
     3001 ttaacacaaa atcttgaagt ttagtttcaa agaacaaagt accttaatcc aaatttattt
     3061 tcataattta ttcacagtaa tttattaaca tttttttgtt tataaatatt taacgacata
     3121 attatttgaa aaccttataa aaattagatt gagcaataaa aaaataacaa aatagtatgt
     3181 ttgatgtgaa taaatttcat atttttaaca aaaaaaatct atggacaaag aattctctga
     3241 acatatgaaa gaagttttat cgtatagccg tgaagaagct atacgtctcg gtaatagcat
     3301 gattgacgct gatcatttgt ttttggggtt acttcgtgaa ggaacttcac aagctgtacg
     3361 cattttagaa gcattacatt gcaatataga atacacaaaa caacaaatag ataattcact
     3421 acgtgaacct atgccactga gtgaagatga gcaagaaaaa attcccctta aacgaacagc
     3481 agataaaata ttaaaaatct ctattttgga atcaaaattt ttaaatcact caaaagttga
     3541 acctgaacat ttattattag cattattaaa agaaaataat tcattatcgg cagaaattat
     3601 gaacaaacaa gatattacat acgaaaaagt aaaacaacac atacaaatac caaatcctga
     3661 aaataaatct caaaatccat ttggtgatga tgatgacgat atgtcaaaat cacaaaaaca
     3721 accaaattct ccaaaaaccg aaaaaccatc ggaaacacca gttttagata attttggagt
     3781 agatttaacc aaagctgcag aagaaggtag attggatttg gtagttggtc gtgaaaaaga
     3841 aattgaacgt atagctcaaa ttttaagtcg tcgaaaaaaa aataatccga ttttaattgg
     3901 agaacccgga gtaggaaaat ctgcaattgc agaaggttta gctttgagaa ttgtacaaaa
     3961 acaagtatcg cgtatattat ttaataaacg tgtgcttaca cttgatttgg cttctatagt
     4021 agcaggaact aaatatagag gtcaatttga agaacgtatg aaagctatgc ttaatgaatt
     4081 agcacgttca aaagatgtta ttttatttat tgatgaaata cataccattg ttggcgctgg
     4141 tggtgcttcg gggtcattag atgctgcaaa tatgcttaaa cctgcattgg ctcgtggaga
     4201 gattcaatgt attggagcaa ctacgcttga tgaatacaga caacatattg aaaaagacgg
     4261 agcattggaa cgtcggtttc aaaaagtaat ggtagaaccc gcaacagttg atgaagctat
     4321 taatattcta aataatatta aaaaacatta tgaaaaacat catggtgtgt attataccga
     4381 tgatgctata aaagcatgtg tatatttaac tcacagatat ataaccgaca gacatctgcc
     4441 agataaagct attgatgctc ttgatgaagc agggtctcgt gtccatattc aaaacattca
     4501 agttccaact acaatagtat cattagaaaa agaaattgaa gaattaaaac aaaagaaaat
     4561 agatgctgtt cgcaatcaaa attttgaatt ggctgcaagt taccgagaca atgaaaaaat
     4621 gcttataaag caaattgaag aagaaaataa aaaatggcaa gatatgcttc t
//
LOCUS       DAOTOW010000079         4655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15158_length_4655_cov_3.227391, whole genome shotgun sequence.
ACCESSION   DAOTOW010000079 DAOTOW010000000
VERSION     DAOTOW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15158_length_4655_cov_3.227391"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..508)
                     /locus_tag="P5243_02050"
     CDS             complement(86..508)
                     /locus_tag="P5243_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRS18260.1"
                     /translation="MNISQKRILFVGGGAVCLHKILAVQKFTSSITIVSKEFLPEVEA
                     LGFHCITKEYDEHDLDTVNIVYACTNNTETNKRIHADAKRRGLLINVADNPSLCDFIS
                     PAVHTQGNITVAVSSGGTDVKKAVRIRNMIREFLTEHL"
     gene            complement(529..1350)
                     /locus_tag="P5243_02055"
     CDS             complement(529..1350)
                     /locus_tag="P5243_02055"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HRS18261.1"
                     /translation="MNIELSHIPLREQKPRSKGLTVLEDTGESIAAVEQLLQTAAHCI
                     DYVRLTPQSFVSQQALQERILLYSSYSVISFVSGLLFEAAYLRSSVDQFLNTLEKLSI
                     KCIEISDGTIEIPFTEKSQIIQKCSKKFDVVTKIGAAKKEYKFHTDNWRLYIDSALQS
                     GAKRIMIEGGEAGASQLINGQLQINEILAHGISKYAPAESLIWEAPEITQQIWLLNQY
                     GSTVNIAGIQSHKILEMETVRQGLSIHTLLQYLPPQLSVGKVREFDTIYNFDWQI"
     gene            complement(1362..3143)
                     /locus_tag="P5243_02060"
     CDS             complement(1362..3143)
                     /locus_tag="P5243_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS18262.1"
                     /translation="MRYKYTKAKNFSEFTTRNGVVNTPLDFKTFAKDIPCQHACPAKT
                     NVPLYIEQIFQGNYDAAYRINLEDNVFPGVLGRVCTRPCESACRHNWTNIQGPVQICH
                     LKRISADFFKTPIEPLVPWYEKTGKHIAIIGGGPAGLAAARELFRFGHTVTIFEREPR
                     LGGMMIHGIPRFRLPAEIIEAEIDIIIRSGINIKLNSNITYQTLQTIQSEYDAVLLTT
                     GTSLASHIELDIDADNCMYGNDFMYAFNTNTLAPISGNVLVIGGGFTAVDCARSCARA
                     ARKLMGEQGTVSIVYRRNQENMAANFEELEEIVHEHISIQTLCTPVGIVKENNTITGV
                     TFVKNKLEVSPKGGKPNMKPIPGSEFTQACDYVIVAIGQTQDYSILGSYSSADEACAS
                     QKKLFSAGDFRTGGKDVITAVADAKQTARQIDEFLMGTKRRSVHIAVQMYENEYDGKT
                     GRTRDFDLQWLQTMPTAPLKQRAQNPDTEVEKGFDLQQHTSVSSRCYLCHYKFEIDHD
                     KCIHCNWCIEVTPRKCISRVSHVFFDEDGSPVDYVKSNTAADGTYIHIDSNECIRCGK
                     CLRVCPTEAISMVKMTKIECSNNITKA"
     gene            complement(3133..>3349)
                     /locus_tag="P5243_02065"
     CDS             complement(3133..>3349)
                     /locus_tag="P5243_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18263.1"
                     /translation="QGSVEGYEMRIGGSHTSFGKIVGEFKTADIPEITETVLDTFIKL
                     RNDSETLAQTVQRIGVEPFKEAVLYAI"
     assembly_gap    3350..3359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3360..>4655)
                     /locus_tag="P5243_02070"
     CDS             complement(<3360..>4655)
                     /locus_tag="P5243_02070"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRS18264.1"
                     /translation="GFVLDFDEIARKGDMSKEEKLIAKWYGVYTSRQAGNFMARVVIP
                     GGIMTAQQARCLASTSFKYGQGIMNITTRQAIQFHWLKLGHLADMMRELNTAHLTTFH
                     GCGDVTRIVAACPLAETCAYARFDVRTYAVATQKYLGSFRDLDNLPRKFKITYSGCNT
                     GCGQPYINCVGIVAQQAEINGKTEFGFKVIIGGGLGWKQFIGQELFSFVPQNQILEVT
                     RAVALLYRDYGDRTNRIKSRLKYVVHRNGIDFCREIVLANLKAEGKHIDNILHTSIRE
                     TGGDVPYRIQESMYAAADGTTTVGIRVPKGELSYRQMNSIAELSEEYGNQHVYTTNRQ
                     NLELKGVKPADVLTLQQRIRDIGLHTDGFYGIQDIVPCVGITYCPKAVSETRALHDNL
                     QPILQLKKFESIRNSVLINISGCPNSCSQYSIFYIGYRGM"
BASE COUNT         1300 a          960 c          838 g         1547 t
ORIGIN      
        1 acaaaggctc taaggctcaa aggttttcta ttataattta tagttattgc cctttttgtg
       61 tcttggtgaa ttagtggcca atttactaca aatgctccgt caaaaattca cgtatcatat
      121 tgcgtattct cactgccttt ttcacatcgg ttccaccgga gcttaccgct accgttatat
      181 ttccttgggt atgtactgca ggcgaaataa aatcacacaa cgacggatta tctgctacat
      241 ttatcaataa cccacgacgt tttgcatcgg catgaattcg cttgttagtt tcggtgttgt
      301 tggtacaggc atatactata ttgacagtat ccaagtcgtg ttcatcatat tctttggtaa
      361 tacaatgaaa tccaagtgct tctacttcgg gcaaaaattc tttggaaact atcgtaatag
      421 atgaggtgaa tttttgtact gctaaaattt tgtgcagaca cactgcccct cctcctacaa
      481 agagtattcg cttttgtgat atgttcaaat ttatgggtaa atacattgct aaatttgcca
      541 atcgaaatta taaattgtat cgaattcgcg aacctttcct acactcagtt gcggtggaag
      601 atattgtagc aaagtgtgta ttgataatcc ttggcgaaca gtttccattt ctaatatttt
      661 gtgcgattga attccagcaa tattaacggt gctgccatac tgattgagca accatatttg
      721 ttgagtaatt tcgggggctt cccaaatcaa ggattctgcg ggggcatact ttgaaatacc
      781 gtgtgcaagt atttcattta tttgcaattg accatttata agttgcgatg ctccggcttc
      841 accaccttct atcattatac gtttagcgcc tgactgcagt gcactatcta tatatagtcg
      901 ccaattgtcg gtatgaaatt tatactcttt ttttgctgct ccaattttag taacgacatc
      961 aaatttttta gaacattttt gaataatttg tgatttttcg gtaaacggta tttctattgt
     1021 tccgtccgaa atttcaatac atttgattga aagtttctca agtgtattta ggaattgatc
     1081 aacacttgaa cgtaaatatg cagcctcaaa caataaccca cttacaaatg aaatcacaga
     1141 atacgatgaa tacagcaaaa ttcgttcttg tagtgcttgt tgcgaaacaa acgattgtgg
     1201 tgtaagtcgc acataatcta tacaatgagc agcggtttgc aataattgtt caactgctgc
     1261 tatactttct cctgtatctt ccagtacagt caatcctttg gaacgcggtt tttgttcgcg
     1321 taatggaata tgtgataatt ctatattcat aattctaggt attaggcttt tgttatattg
     1381 ttagaacatt caatttttgt cattttcacc atgcttatag cttcagttgg gcacacgcgt
     1441 aaacatttgc cacaacgtat acattcattg ctgtcaatat gaatatacgt tccgtcggca
     1501 gcagtgttag atttcacata atctaccgga gaaccgtctt catcaaaaaa cacatgcgac
     1561 actctcgata tacatttacg tggagttact tctatacacc aattgcagtg aatacacttg
     1621 tcgtgatcta tctcaaattt ataatgacac aaataacatc gtgaacttac cgatgtatgt
     1681 tgttgcaaat caaatccttt ttctacttcg gtatcggggt tttgtgcacg ctgcttaagt
     1741 ggagcagtag gcatagtttg aagccattgc aaatcaaaat cgcgtgttct accggtttta
     1801 ccatcatatt cgttttcgta catctgcaca gcaatatgca cactacgacg ttttgtcccc
     1861 atcaggaatt catctatctg tcgggctgtt tgttttgcat cggctaccgc agttatcaca
     1921 tctttaccgc ctgttctaaa atctcctgcc gaaaataact ttttctgtga tgcacacgct
     1981 tcatcagctg atgaatatga ccctaaaata ctgtaatctt gtgtttgacc aattgcaaca
     2041 atcacatagt cgcatgcttg ggtaaattcg ctgccgggta ttggtttcat atttggtttt
     2101 cctcccttag gcgaaacttc caatttattt ttcacaaatg tcaccccggt tattgtattg
     2161 ttttctttta ctatacctac aggcgtacaa agtgtttgaa tagatatatg ttcgtgtaca
     2221 atttcttcta attcttcgaa atttgcagcc atgttttctt gatttctgcg atacacaatg
     2281 cttacagtcc cttgttcgcc cattaatttt cgcgcagcac gggcacatga acgagcacag
     2341 tctacagccg taaaaccgcc accaattacc agtacattgc ccgaaattgg cgccaatgta
     2401 ttggtgttga atgcatacat aaagtcgttg ccatacatgc aattgtcggc atctatatct
     2461 aattctatgt gcgatgccaa tgatgtaccc gtagtaagta acacagcatc gtattccgac
     2521 tgtattgttt gtaatgtttg gtatgtaata ttgctattaa gcttgatatt gattcccgaa
     2581 cgaataatga tatcaatttc tgcttcaata atttcggcag gtaatcgaaa gcgaggaatg
     2641 ccgtgaatca tcatgcctcc taatcgaggt tcgcgttcga atattgttac ggtatgtcca
     2701 aatcggaaca attcacgagc tgcagcaagc ccagccggtc caccaccaat tatagcaata
     2761 tgttttcctg ttttttcgta ccatggcact agtggttcta taggtgtttt aaaaaaatca
     2821 gcagaaatgc gtttgagatg acaaatttgt accggtccct gaatattagt ccaattgtgg
     2881 cggcacgcac tttcgcacgg acgagtacac actcttccta aaacgccggg gaacacatta
     2941 tcttcgagat tgatgcgata tgctgcatcg taatttcctt gaaatatttg ctcaatatac
     3001 aatggcacat tggttttggc agggcatgca tgttggcagg gaatatcttt ggcaaatgtt
     3061 ttgaaatcca aaggggtatt aaccactcca tttcgagtag taaattcaga aaaattcttt
     3121 gcttttgtat atttatatcg catacaacac tgcttcttta aatggttcaa ctcctattct
     3181 ttgtactgtt tgtgccaatg tttccgaatc atttcgtagt tttataaatg tatcgagcac
     3241 ggtttcggta atttcgggta tatcggcagt tttaaattcg cctacaattt tcccaaaact
     3301 tgtatgcgag ccacctatac gcatttcata accttctacc gaaccttgtn nnnnnnnnnc
     3361 atgcctctgt aaccaatgta gaatatagaa tattgcgaac aagaattggg acaaccagaa
     3421 atgttgataa gaaccgaatt tcgaatggat tcaaattttt ttaattgtaa aatcggctgt
     3481 aaattatcat gtaatgctcg tgtttcgctt acagctttgg gacaatatgt aattccaaca
     3541 caaggcacaa tatcttgtat tccgtaaaat ccatcggtat gcaaacctat atcccgaatt
     3601 cgctgttgta aagttagcac atcagcaggc tttactcctt taagttctaa attctgacga
     3661 ttggtggtat acacatgttg gttgccatat tcttcggaga gttcagcaat gctattcatc
     3721 tgtctatatg aaagttctcc ttttggtact cgtataccaa cagttgttgt accatctgca
     3781 gcagcataca tagattcttg aatacggtat ggcacatcac caccggtttc gcgaatagat
     3841 gtatgcaata tattatcaat gtgttttcct tcggctttta aatttgcaag gacaatctct
     3901 ctgcaaaaat ctattccatt acggtgtact acatatttca aacgcgattt tatgcgattt
     3961 gttctgtcgc catagtcacg atagagtaat gctacagctc gggtaacttc taatatttgg
     4021 ttttgtggaa caaacgaaaa taattcttgc ccaataaatt gtttccaacc caagccacca
     4081 ccaataataa ctttgaagcc aaattcggtt ttaccgttaa tttctgcttg ttgcgctaca
     4141 attcccacac aatttatata tggttgtccg cagcctgtat tacaccccga ataagtaatt
     4201 ttgaattttc ggggtaaatt atctaaatca cggaacgaac ctaaatattt ttgggttgcc
     4261 accgcatatg ttcgtacatc aaatcgggca tacgcacaag tttcagctag cggacatgct
     4321 gcaactatac gagttacatc gccacaacca tgaaaagttg taagatgagc ggtattcagt
     4381 tcacgcatca tatcggccaa atgccccaat ttaagccaat gaaactgtat agcttggcgt
     4441 gtggtaatat tcattatacc ttgcccgtat ttgaatgatg tagatgctaa acaacgtgct
     4501 tgttgagcag tcataattcc accgggaatt actacccgcg ccataaaatt tccggcttga
     4561 cgtgaagtat acactccata ccatttcgca attaattttt cttctttcga catatcgcct
     4621 ttacgggcaa tttcatcgaa atcgagtaca aaacc
//
LOCUS       DAOTOW010000080         4624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15269_length_4624_cov_3.417159, whole genome shotgun sequence.
ACCESSION   DAOTOW010000080 DAOTOW010000000
VERSION     DAOTOW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15269_length_4624_cov_3.417159"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="P5243_02075"
     CDS             <1..255
                     /locus_tag="P5243_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18265.1"
                     /translation="RMRKFTDSILVCDDSGCTIIPKEKCTDEGLEKLEFIELQEDIWY
                     FCLDTLKWDTYTTICKKEYLKNKDILPKHLQEKISKYTLD"
     gene            257..1276
                     /gene="pseI"
                     /locus_tag="P5243_02080"
     CDS             257..1276
                     /gene="pseI"
                     /locus_tag="P5243_02080"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HRS18266.1"
                     /translation="MSSHSKTFIIAELSANHNHSFDVAVQSIKAAKQAGADAIKLQTY
                     TADTITLNCDNEYFQIKHGSIWDGTTLYKLYEQAYTPWEWHKDLFEIAQDEGLVCFSS
                     PFDITAVDFLETLNCPIYKIASFEIHDIPLIEYVASKGKPIIISIGIATLPEIDEAID
                     ACRRMGNNDITILACTSSYPAPFDQAHIARIPEIAKRWNVKPGLSDHTMGSTVALGAV
                     ALGAVIIEKHFIIDRNIGGPDASFSMNAIEFSEMVQQIRILEQAIGKPDFELSELSKQ
                     SRWAARSLFVCEDIQEHEVITSTNVRSIRPGHGLHPRFLPLVLGKKAKHVLQKGTPLS
                     WDMLY"
     gene            complement(1316..1714)
                     /locus_tag="P5243_02085"
     CDS             complement(1316..1714)
                     /locus_tag="P5243_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS18267.1"
                     /translation="MKKLVFIVSLLVIATIGYSQTETKKPEVVFDSLTYNYGVIAKGS
                     PGECSFKFTNKSKTPLILTNVKAGCGCTVPSWPREEIKPKHSGEIKIKYNTNNVGYFT
                     KTISVYTNKQEEPIILTITGQVVDEQPKTE"
     gene            complement(1737..2096)
                     /locus_tag="P5243_02090"
     CDS             complement(1737..2096)
                     /locus_tag="P5243_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRS18268.1"
                     /translation="MKRTLIIGASTNPERYSYKAAHKLTENGHEICMIGPRQGELCNT
                     PIHTDLIPFENIHTVTLYVSAKNQIPYYDYIIQLHPQRVIFNPGTENEEFAKKLESLG
                     IIPEVACTLVLLSTNMY"
     gene            complement(2578..2856)
                     /locus_tag="P5243_02095"
     CDS             complement(2578..2856)
                     /locus_tag="P5243_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18269.1"
                     /translation="MKIHTAVLLTSLFAIIQAIVEIIFNKIHQGDSKTKLFKIMALRS
                     TKFLLYLIISLAFILSFPHEQTPISFLVISLFTVYTVFEIVFIIKRSQ"
     gene            complement(3097..>4624)
                     /gene="scpA"
                     /locus_tag="P5243_02100"
     CDS             complement(3097..>4624)
                     /gene="scpA"
                     /locus_tag="P5243_02100"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRS18270.1"
                     /translation="PPAFSMKIIADIFKYTSEKMPKFNSISISGYHMQEAGATNDIEM
                     AYTLADGLEYLRTGINAGLDIDAFAPRLSFFWAVGMNHFMEIAKFRAARMIWAKLVQQ
                     FNPKNPKSLALRTHSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTNALDEAI
                     ALPTDFSARIARNTQIYIQQETEICRAIDPWAGSYYVEKLTHEIAHKAWALIQEVEKL
                     GGMAKAIETGIPKMRIEEAAARKQARIDAGLDAIIGVNKYRLEKEDPIEILDIDNTAV
                     RLAQIERLKKLRENRNEQECQAALAAITKCAETGEGNLLELAVEAAKKNASLGEISDA
                     CEKVAGRYKAVIRSISGVYASEAKNDGDFQKAVELCKKFAAKEGRQPRIMVAKMGQDG
                     HDRGAKVVSTGYADIGFDVDVGPLFQTPEEAAKQAVENDVHILGVSSLAAGHKTLVPQ
                     VIEELKKLGREDIMVICGGVIPAQDYDFLYKAGAVAIFGPGTSVAKAAVQILEILLDE
                     "
BASE COUNT         1489 a          749 c          750 g         1636 t
ORIGIN      
        1 cggatgagaa aatttactga ttcaatatta gtttgtgatg attcgggttg tacaattatt
       61 ccaaaagaaa aatgtacaga tgaaggatta gaaaaattag aatttattga attacaagaa
      121 gacatatggt atttctgttt agatacctta aaatgggata catatacaac aatttgtaaa
      181 aaagaatatc ttaaaaataa agatatttta ccaaagcatt tacaagaaaa aatttctaaa
      241 tataccttag attaacatga gttctcactc aaaaactttt ataattgccg aactttctgc
      301 caatcacaat catagttttg atgtggcggt tcaaagcatt aaggctgcaa aacaagccgg
      361 tgctgatgct attaaactgc aaacgtatac tgccgatact ataacgttaa attgtgataa
      421 tgaatatttt caaataaaac atggaagtat ttgggatgga actacattgt ataaattgta
      481 tgaacaagca tatacacctt gggaatggca caaagactta tttgaaattg ctcaagatga
      541 aggcttagtc tgtttttcaa gtccatttga cataacagct gttgattttt tggaaacgct
      601 taattgtccc atttataaaa ttgcaagttt tgaaattcat gatattcctt tgatagaata
      661 tgttgcttca aaaggtaaac caattattat ctcaatagga atagctacat taccggaaat
      721 agatgaagct atagatgctt gtcgtagaat gggtaacaat gatattacta tattagcgtg
      781 tacatcgtca tatccggcac cgtttgacca agctcatata gcacgaattc ctgaaattgc
      841 aaaacgatgg aatgtaaagc ccggtttaag cgaccatact atgggaagta cagttgctct
      901 tggggcagtt gctcttgggg cagttataat tgaaaaacat tttataattg atagaaatat
      961 tggagggcca gatgcttcgt tttcaatgaa tgcaattgaa ttttctgaaa tggttcagca
     1021 aatacgtata cttgaacaag ctataggtaa acctgatttt gaattatcgg aattatctaa
     1081 acaaagtaga tgggctgcac gttccttatt tgtgtgtgaa gatattcaag agcatgaagt
     1141 tataacgtct acaaatgtac gttctatacg ccccggacat ggtttgcacc ctcgattttt
     1201 accactggta ttgggtaaaa aagcaaagca tgtcttacaa aaaggaactc ctctttcttg
     1261 ggacatgctt tattaattcg cttttgcgaa tctttaagaa atactcaaac tatagttatt
     1321 ctgtttttgg ttgttcatca accacttgac cggtaatagt aagaataatc ggttcttctt
     1381 gtttgttggt ataaacagat atagttttag taaaatatcc gacattatta gtattatatt
     1441 ttatttttat ttcaccagaa tgttttggtt taatttcttc acgtggccaa cttggtactg
     1501 tacatccaca tcctgctttt acgtttgtaa gtataagcgg agttttactt ttgttggtaa
     1561 atttaaaaga acattctccc ggcgatccct ttgctattac accataatta taggtaagag
     1621 aatcaaatac aacttcaggt ttttttgttt ctgtttgtga gtaacctata gttgcaataa
     1681 ccaataaact cacaataaat actaattttt tcatatagac tattgtttaa aatttgttaa
     1741 tacatgtttg ttgataataa tactaatgta catgccactt cgggtattat tcctaatgac
     1801 tccaattttt tagcaaactc ttcattttct gtgccaggat taaaaattac acgttgagga
     1861 tgtaattgta taatatagtc ataataagga atttgatttt ttgcagatac atacaaagtt
     1921 accgtatgta tattttcaaa aggaattaaa tctgtatgta ttggagtatt acataattca
     1981 ccttgtctag gaccaatcat acaaatttcg tgtccatttt cagtaagctt gtgggcagct
     2041 ttatatgaat atcgttcagg atttgtactt gctcctatta ttaaagtacg tttcatattt
     2101 tatagtatta aaaaataact atacaaatgt atataaaaca tgataacgtt attcttttta
     2161 tatgattatt tttagattta aaatattttt ttacacggtt gtccgatata aatatcacaa
     2221 tattaagaat accgaattcc ggatttacca aaaagccatt caattaagta atcgttaaaa
     2281 aataatgaag ttgatgcgta gttttagcag cttgcccaac actttgttta atagtttatt
     2341 acaggacttt catgggcatt ttgctaaaac agcatcaacg aatatatttt tagattacgt
     2401 attgcatggc ggcacttttg cttacttttt tgctgtagaa aaagtaagaa gaagtatgaa
     2461 aaaaaaagta agcctctaaa tttttgatac tttgcttatt cagtattaat gatacttttc
     2521 ggataatcct cgaaatttat ttcggacaac aatatttttt tatacaaaaa tatgtattta
     2581 ttgagaacgt tttattataa aaacaatttc aaataccgta tatacagtga acaaagaaat
     2641 aactaagaat gaaattggtg tttgttcatg aggaaatgat aagataaagg ctaatgaaat
     2701 aattaaatat aacaaaaact tagtgcttcg taatgccatt attttaaaaa gtttagtttt
     2761 agaatctcct tgatgtattt tattaaaaat aatttcaaca attgcttgaa ttatagcaaa
     2821 taatgaagtt aacaaaacag cggtatgtat tttcatacac atcatgttaa gtgaatgtaa
     2881 aatatataca ctacaaataa atgctaggta tacaatacta tgagttgcta ttctgaatat
     2941 aatgtgcggc ataatttctt tttttacaaa gctacaaaat gaataatgtt acattctatc
     3001 tatagtttaa ttttaagtta ataatttgta aactatatac ataaaaaaag ctgaactaca
     3061 atgttcagct tttttatata aattaaatga atgtatttat tcatcaagta atatttcgag
     3121 aatttgaact gcagctttgg ctacagaagt acccggtcca aatatggcaa cagctcctgc
     3181 tttgtataag aaatcatagt cttgagcagg aattacccca ccacatataa ccataatatc
     3241 ttctctgccg agttttttaa gttcttctat tacttgagga accaatgttt tatgacctgc
     3301 tgctaacgat gaaacaccta aaatatggac atcgttttct acagcttgtt ttgctgcttc
     3361 ttcaggagtt tgaaatagtg gtcctacgtc tacgtcaaaa cctatatcgg catagcctgt
     3421 agatacaact ttggcaccac ggtcatgacc gtcttgtccc attttagcaa ccattatacg
     3481 tggttgacgt ccttcttttg cggcgaattt tttgcacagt tctactgctt tttggaaatc
     3541 gccatcgttt ttagcttctg atgcgtacac tcctgaaatg cttctaatta ctgctttata
     3601 acggcctgct actttttcgc aagcatctga aatctcacca agcgatgcat ttttcttagc
     3661 tgcttctact gctaattcaa gtaagtttcc ttcacctgtt tctgcacatt tggtaatagc
     3721 ggcaagtgct gcttgacatt cttgttcatt acgattttca cgtaattttt tcaatcgttc
     3781 tatttgtgct aaacgaacag ctgtattgtc tatgtcaagt atttcaatag gatcttcttt
     3841 ttctaatcta tatttattta ctccaattat agcatcaagt cctgcatcta ttcgagcttg
     3901 tttgcgagca gcagcttctt caattctcat ttttggaatt cctgtttcta tagcttttgc
     3961 cataccacca agtttttcaa cttcttggat taacgcccat gctttgtgag ctatttcatg
     4021 tgtaagtttt tctacataat atgaacctgc ccaagggtca atagctctac aaatttctgt
     4081 ttcttgttga atataaattt gtgtattacg tgcaatacga gccgaaaagt ctgtaggtaa
     4141 tgcaatagct tcatctaacg cattagtatg taatgattga gtatgtccta atgctgcagc
     4201 cattgcttct atacaagtac gtcccacatt attgaacgga tcttgttcgg taagcgacca
     4261 acccgatgtt tgtgagtgcg tacgtaatgc aagcgatttt ggatttttag gattaaactg
     4321 ttgaaccagt tttgcccaaa tcatacgagc tgcacggaat tttgcaattt ccataaaatg
     4381 attcattccc actgcccaga aaaatgataa acgtggtgca aaagcatcta tatctaatcc
     4441 tgcattaata cctgttctta aatattccaa cccatcagct aaggtatatg ccatttcaat
     4501 atcattggta gcacctgctt cttgcatgtg atagcctgat atagaaattg aattgaattt
     4561 tggcattttt tccgatgtat atttgaaaat atcagctatg attttcatag aaaatgcagg
     4621 cgga
//
LOCUS       DAOTOW010000081         4585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15431_length_4585_cov_3.004415, whole genome shotgun sequence.
ACCESSION   DAOTOW010000081 DAOTOW010000000
VERSION     DAOTOW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15431_length_4585_cov_3.004415"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2254)
                     /locus_tag="P5243_02105"
     CDS             complement(<1..2254)
                     /locus_tag="P5243_02105"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS18271.1"
                     /translation="MNSVRYVYVLFIISILVYTGYSQTIPNALPGLQAWWSADSIKVG
                     NGNPIETWTTNNNEFLFVTQPTAANRPTLVNNVINGKPVVRFDGVNDYLNGGDILDIQ
                     NDGQTIFVIGISKSNNGIFVTKSNLIEVPARYTLGFSVSEINYIYHDNTRYFIKDISP
                     LNTFNIFCGLADKINGIARLIVDSRILKTQTIAKNYNMNSSFEFLIGAFNNSIGSPPY
                     PGFYLNGDIAEIIIYNRPLAQLERQEIENYLRMKYFPGTERLQFSLGPDIHEPYSLAP
                     ITLTVPPKPYYVSYLWSTGATTPSIQVNSSGTYWVKAIDDWGYEYFDTINITKPSLTQ
                     LQNQILCKGSSITWDCGISGAYTYTWSTGASAQSITITEGGTYSVTVTDSFNNSIVSQ
                     PVTITIDNFSELASLGPDKALCRGNTIELVSQKDKATSYIWSTGATSPEIIFNTAGEY
                     WVQVSNANNCTKRDTIVLTLKGIAPFVDFAASHICEGETTELTQQAVPLDASEIVAAT
                     WIIQTDTLHGFSVSYNFPNQGDTPVKLTVQTDQDCYGTLVKNVFIAPNPTVEFIPPKA
                     CQNGITTFESKATVPTGTIDEWTWTIEGEQFTGQTIQYTFSQTGSFGVKLAVKSDMGC
                     KGSLIKPIQVREAPLVQFVTTKTCQNEPIVCFDKTEYVSYNTVVDGAWIIDGVRNTYN
                     TAVSKTFTDKLQHTITLQIQTTNGCINSHTKTIIPNPAPQTLIPPLFGCIHKDLQVED
                     ASNTYGNTVDV"
     gene            complement(3331..3705)
                     /locus_tag="P5243_02110"
     CDS             complement(3331..3705)
                     /locus_tag="P5243_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRS18272.1"
                     /translation="MKTLNAISYDIIGCAYKVHSELGPGLLESTYETCLYYELLQMGY
                     SISRQQALPIIYKEQKLDAGYRIDLLVENSIIVELKSVDTIIPIFEAQLLTYLKLAHK
                     KLGLLINFNVCDLKQGIIRKIL"
     gene            4016..>4585
                     /locus_tag="P5243_02115"
     CDS             4016..>4585
                     /locus_tag="P5243_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS18273.1"
                     /translation="MGKPILEITNISKQYRLGYVGTGSLGHDIHRWWCNVRGKEDPYL
                     KIGEENNRTQVGTSEYVWALRDINLQVQAGEVLGIIGANGAGKSTLLKILSRVTGPTT
                     GKITARGRIASLLEVGTGFHGELTGRENIYLNGAILGMTKKEITSKLDEIIDFSGCER
                     YIDTPVKRYSSGMYVRLAFAVAAHLEPEIL"
BASE COUNT         1562 a          742 c          792 g         1489 t
ORIGIN      
        1 aaacatctac agtatttcca taagtattac ttgcatcttc tacttgtagg tctttgtgaa
       61 tacacccaaa cagagggggg attagggttt gcggagcagg atttggtata attgtttttg
      121 tatgcgaatt tatacatccg ttagtagttt gtatttgcag agtaatagta tgttgtaatt
      181 tatcggtaaa agttttacta acggcagtat tatatgtgtt tcgtacgcca tctattatcc
      241 atgcaccatc tactacggta ttgtatgaaa catactcggt tttgtcgaaa catactatag
      301 gttcattttg gcatgttttg gtagtaacaa attgtactaa tggagcttcg cgaacttgta
      361 taggttttat aagagaacct ttacaaccca tatcactttt tacagctaat ttaactccga
      421 aagaaccggt ttgtgaaaaa gtatattgaa tagtttggcc ggtaaactgt tccccctcaa
      481 tagtccaagt ccattcatct atagtacctg taggaacggt agctttggat tcaaacgttg
      541 taataccgtt ttgacatgct ttaggcggta taaattcaac tgttggatta ggagcaataa
      601 atacattttt tactaacgtt ccataacagt cttgatctgt ttgaacagtt aatttaacag
      661 gggtatcacc ttgattgggg aagttataac ttacactaaa tccgtgtaga gtatcggttt
      721 gtataatcca agtggcagct acaatttctg atgcatcaag aggaacagct tgttgagtta
      781 gttctgtagt ttcaccttca catatatgtg atgctgcaaa atctacaaac ggagctatac
      841 cttttagtgt caatactatg gtgtcgcgtt tagtacaatt gttagcatta cttacttgca
      901 cccaatattc accggcagta ttaaaaataa tttcgggtga agtggctcct gtactccata
      961 tatacgaagt agctttatct ttttgcgaaa ctaattcaat agtattgcca cggcacaatg
     1021 ctttatcagg tcctaatgaa gctaattccg aaaaattatc aatggtaatg gtaacgggtt
     1081 gcgaaacgat agaattatta aaagaatctg taaccgtaac agaatacgtg cctccttctg
     1141 ttatagtaat agattgtgct gaagcaccgg tactccaggt ataggtatat gctccgctaa
     1201 taccgcaatc ccaggtaata gaagaccctt tacacaatat ttgattttgc agttgagtta
     1261 gacttggttt tgtaatattt atagtgtcaa agtattcgta cccccagtca tcaatagctt
     1321 taacccaata tgtccccgaa gaatttactt gaatacttgg tgtagtagca ccggtactcc
     1381 ataaatagct tacgtaatag ggtttaggag gtacggtaag ggtaatagga gcaaggctat
     1441 atggttcatg tatgtcgggt cccaaactaa attgtaatcg ttctgttccg ggaaaatatt
     1501 tcatgcgcag gtaattttca atttcctgac gttcaagctg tgcaagcggc cggttgtata
     1561 tgatgatttc tgctatgtcg ccgttgaggt agaagcccgg atatggagga gagccaattg
     1621 aattattaaa tgcaccaatt aaaaattcaa aagaagaatt catattataa ttttttgcta
     1681 ttgtttgagt ttttaaaatt ctgctatcaa ctatcaaacg agctattccg tttattttat
     1741 ctgccaaacc acaaaagata ttaaaagtat taagaggaga aatgtctttg ataaaatatc
     1801 gagtattatc gtgataaata taatttattt cacttacaga aaagcctaga gtatatcttg
     1861 ctggtacttc tataagatta gattttgtaa caaaaatacc gttattagat ttagatatac
     1921 cgataacaaa tattgtttga ccatcatttt gtatatctag tatatcacca ccatttaaat
     1981 aatcattcac cccatcaaaa cgcactacgg gttttccgtt aattacgtta ttaactaaag
     2041 taggacgatt tgcggcagtt ggttgtgtta caaacaagaa ttcattattg tttgttgtcc
     2101 atgtttcaat agggttacca ttgcctactt taatagaatc cgcgctccac catgcttgta
     2161 atccgggcaa agcattggga atagtttggc tatatccggt atatactagg atagaaatta
     2221 tgaataaaac ataaacataa cgcacagaat tcatgcaaaa taattgttta ataacacaaa
     2281 gatagaaaaa taaatgttta atgtataatt tttactgttt aattcgcctt ggaatacata
     2341 ttatacacat tctattaaat tatgattctt cacagatatc acagaataat aaaaaataat
     2401 acatataaca atctgtgaaa atctgagtaa tctgtggtac aaagatatta tttaaattta
     2461 aaagagtata ccacagatgt cacagataac cacagattaa aacagaaaat aatctttgta
     2521 tttgtaaaat gggtaatacg tttttaaaac aacgctatag ctattctctt aatgagactt
     2581 ttttgtgagc gatattattg aacagtattt gtatgtgttt acaaagatat acggaaacat
     2641 atattatttt cttttttatt tgatatatat ggaattgtaa aaaagaagag attattcttg
     2701 cgggaacaat ctcttcgggt actcctcttt ttttcaggtg ttggttgaaa aaaagcatca
     2761 ttttgtcaac acatctattt tagacaatac ttgaattaaa atacatgtat tattggtttg
     2821 cgtactgcaa atatatataa ttcctcttac tgcacaagga tttcggataa aattttgtac
     2881 tattttttac atctttttac gaacatagta ggtatatttt tgaatacgaa tatgttaggg
     2941 tttttagatt ttcaaaatat tttattatag aaatcacaga ttcacacaga aattaaaaat
     3001 aatctgtgtg aatctgtgta atccgtggta gaaagtagct aaaaaataat ttcaccacag
     3061 atatcacaga ttaacacaga ttaaaataca aatcaatctg tgaaaatccg tgtaatctgt
     3121 ggtaaaaaat gtagctaaaa aacaaaaact actacaccac agatatcaca gattagcaca
     3181 gattaaaata caaatcaatc tgtgaaaatt cttgtaattt gtagtaaaaa atgtaactaa
     3241 aaactactac accactgata tcgcagatta acacagatta aaatagaaat caatctgtga
     3301 aaatccgtgt aatctgtggt aaaaaagaac ttataaaatt tttcgaataa ttccctgttt
     3361 caaatcgcac acattgaaat tgataagtaa acctaatttt ttatgtgcta acttcaaata
     3421 tgttagaagt tgagcttcga aaatgggaat tatagtgtca acagatttga gttcgactat
     3481 tatagaattt tcaactaata aatcaattct atatcccgca tcaagttttt gttctttata
     3541 aattataggt aaagcttgtt gcctagaaat agagtatccc atttgtaata attcataata
     3601 caaacaggtt tcgtatgtgc tttctaataa tccgggaccc agttcagaat gaactttata
     3661 ggcacagcca attatatcat acgatatagc atttaatgtt ttcattgata tttgtgttac
     3721 ggttatatgc aaaaatatat aatatataga gataaacaaa aaactactac accacagata
     3781 tcacagattc tcacaaaaat taaaaataat ctgtgtgaat ctgcgtaatc cgtggtagaa
     3841 agtagctaaa aaataatttc accacagata tcacagatta acacagatta aaatagaaat
     3901 caatctgtga aaatccgtgt aatctgtggt aaaaactgtt tgttttaaac aaaaaataga
     3961 catacatatt atactttgta ttgaatctat tatatttgcg tataaaacta tactaatggg
     4021 gaaaccaatt ttagaaatta caaatatttc caaacaatac cgtcttggtt atgtaggtac
     4081 cggtagcctt gggcacgata ttcatcgctg gtggtgcaac gtgcgaggca aagaagatcc
     4141 gtatttgaag attggcgaag aaaacaatcg cacacaagtt ggtacttcgg aatatgtatg
     4201 ggcattgcgc gatataaatt tacaagtaca agccggcgaa gttctcggca tcataggagc
     4261 caatggagca ggaaaatcta cattgctcaa aattctgagc cgtgtaacag gacccacaac
     4321 aggcaaaatt accgcacgtg gtcgtattgc cagcctttta gaagttggca caggattcca
     4381 tggtgaactt accggacgcg aaaatattta tctcaacggt gccatacttg gcatgaccaa
     4441 aaaagaaatt acctcgaaat tagatgaaat tatagacttt tcaggttgcg aacgctatat
     4501 agatacccct gtaaaacgct atagttcggg catgtacgta cgccttgctt ttgccgtagc
     4561 agcacactta gaacccgaaa tcctg
//
LOCUS       DAOTOW010000082         4553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15540_length_4553_cov_3.761894, whole genome shotgun sequence.
ACCESSION   DAOTOW010000082 DAOTOW010000000
VERSION     DAOTOW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15540_length_4553_cov_3.761894"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="P5243_02120"
     CDS             complement(<1..797)
                     /locus_tag="P5243_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS18274.1"
                     /translation="MNILLIGGTGIISSSCSALAVERGYNVFHINRGNSQNIRPAYGV
                     THIQADIRNFEQCTHILSHYTFDVVIDFISFIPQHIEQNIQLFSGKTKQYIFISSASI
                     YQTPPLTLPVTENTPRENPVWEYSRNKIACEDVLFDAYTNYSFPAVIVRPSHTYDKTL
                     LPIEGGYTVLHRMLQNKPIVIHGDGSSIWTLTHAHDFAKGLVGLCGNTNTLGEAFHIT
                     SDQWLTWNRIYELIGNQLGVTPQVVYIPSTTIARYHKEIGDSLLGDKT"
     gene            complement(813..1277)
                     /gene="pyrI"
                     /locus_tag="P5243_02125"
     CDS             complement(813..1277)
                     /gene="pyrI"
                     /locus_tag="P5243_02125"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRS18275.1"
                     /translation="MGDIQGRHLIVSALKNGTVIDHIPSKSLFKVISILKLENIHSQM
                     TFGTNLESKKLGSKAIIKISDKFFEQEEINKIAIIVPEAKLNIIKNYEVVEKMIVTLP
                     ETIVGITKCFNPKCITNHEKVTPKFTVVEKSPVSLQCQYCEKITVEEHFNLI"
     gene            complement(1285..2196)
                     /gene="pyrB"
                     /locus_tag="P5243_02130"
     CDS             complement(1285..2196)
                     /gene="pyrB"
                     /locus_tag="P5243_02130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRS18276.1"
                     /translation="MTAKSLVSISDYKVEDYNRILQLASEFEANSYQNLLHGRVIATL
                     FFEPSTRTRLSFESAINRLRGRVIGFADIGTTSSTKGESLHDSIRMVSGYCDLIVMRH
                     PLEGAARYASEVSSVPIINAGDGANQHPTQTLLDMYSILKTQGTLQNLAITLIGDLKY
                     GRTVHSLIMAMMNYNPRFYFVSPPELTLPNEYKQILTKHNIPFSEHTDMQEVLDISDI
                     IYMTRVQKERFSEPMEYEKTKNLYVLKNSMLANTKENMKILHPLPRVNEISMCVDANP
                     KAYYFEQAKNGVFTRQAIICHLLGIKI"
     gene            complement(2253..2588)
                     /locus_tag="P5243_02135"
     CDS             complement(2253..2588)
                     /locus_tag="P5243_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRS18277.1"
                     /translation="MSEQLHNEFKEDAYSKSIRAGKRTYFFDIKVTKTNDFFLTIIES
                     KRVFQSEGQFSYEKHKIFLYREDFEKFMEGLNDAFRFVELNKQSELHSLELQDEYKNI
                     EFEDLHEKE"
     gene            2886..>4553
                     /locus_tag="P5243_02140"
     CDS             2886..>4553
                     /locus_tag="P5243_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRS18278.1"
                     /translation="MKKIVIIMVISTMSFACSQKSKSIDEKVNELLSRMTLDEKIGQL
                     VQYNGFWDATGPLPQESNSKRKYNDVRQGLVGSVLNVYGVEQVRKLQEIAVTQSRLGI
                     PLIFGMDVIHGYKTAFPVPIAEAASWNMQTIEQAARISATEASAAGINWTFAPMLDVS
                     RDPRWGRVMEGAGEDPYLTAQVGIAKIKGFQGTDLSHSQTIAACAKHFAGYGFPIAGR
                     DYNTVDVSKVTLYNDILPPFKAAVENNVATVMNAFNVLNGVPCTGNSWLVRDVLKNTW
                     NFKGFVVSDWGSIMEMATHGYAQDRYHAAQLAIEAGCDMDMESYAYRNDLKTLIEKGV
                     VKESLIDDAVRRILRIKFKMGLFENPYKYCDKEHEKVILQNTDYSKEALQSALEAIVL
                     LKNEQSLLPLSKNQKNIAVIGALANDKDTPLGNWRAQAVKNTAVSVLEGISAYTNDYV
                     YAQGALVHTNAAGFTHTVNINTTDKSGFAQAIAVAKKADVIIMVLGENCFQSGEARSR
                     SDIGLPGVQQELLEAVYAVNRNIVLVLMNGRPLTISWAGEHIPAIVEA"
BASE COUNT         1410 a          677 c          905 g         1561 t
ORIGIN      
        1 gttttgtcgc caagcagact gtcgcctatt tctttgtggt agcgagctat tgtggttgat
       61 ggtatatata ctacttgcgg tgtaacgcca agttggttgc ctattagttc atatattctg
      121 ttccatgtaa gccattggtc tgatgtaata tgaaaagctt cgcctaaggt atttgtgttg
      181 ccgcataatc caactaagcc ttttgcaaaa tcgtgggcat gagtaagtgt ccaaatagaa
      241 cttccgtcgc cgtgtattac tattggtttg ttttgcagca tgcggtgcaa tacggtgtag
      301 ccaccttcta tgggtaacaa tgttttatcg tatgtgtgcg atggtcttac aattacggcc
      361 ggaaacgaat agtttgtgta agcatcaaat aatacatctt cgcaggcaat tttgttgcgt
      421 gaatattccc acacgggatt ttcgcgtggg gtgttttctg ttacaggtag ggtaagtggc
      481 ggagtttgat atatagatgc tgaactgata aaaatgtatt gttttgtttt tccgctgaac
      541 agttgtatat tttgttcaat atgctgtgga ataaatgaaa taaaatcaat aactacatca
      601 aatgtatagt gtgaaagaat atgagtacat tgttcaaaat ttcgaatatc agcctgtatg
      661 tgtgttaccc catatgcagg tcgaatgttt tgcgagttgc cacggtttat atgaaacaca
      721 ttgtaaccgc gttctacagc taatgcagaa catgatgaac ttataattcc tgttccgcct
      781 attaagagta tattcatagt gtaattacgg ggttaaataa gattgaagtg ttcttcaaca
      841 gtaatttttt cgcaatattg acattgcaaa cttaccggcg atttttcaac tacggtaaat
      901 tttggtgtta ctttttcgtg atttgtaata catttagggt taaagcattt ggtaataccc
      961 acgatagttt cgggtaatgt tactatcatt ttttcaacta cttcgtaatt ttttatgata
     1021 ttcaatttag cctcgggtac aattatggca attttattga tttcttcttg ttcaaaaaat
     1081 ttatctgaaa tctttatgat agctttactg cctaattttt tactttctaa atttgtacca
     1141 aaagtcattt gagagtgtat attctcaagt tttaaaatag aaattacttt aaacaaactt
     1201 ttacttggaa tatggtctat aaccgttccg tttttgagtg cacttactat gaggtgtcgt
     1261 ccttgaatgt cgcccatgat tcacttatat tttaattcct aataaatgac aaataatagc
     1321 ttgacgagta aaaacgccat tttttgcttg ttcaaaatag tatgctttgg ggttagcgtc
     1381 tacacacatg cttatttcgt ttacacgagg cagagggtga agaattttca tattttcttt
     1441 ggtgttggca agcatagaat ttttgagtac atacaaattt ttagtttttt cgtattccat
     1501 tggttctgag aagcgttctt tttgaacacg agtcatatat ataatatcgc taatgtctag
     1561 tacttcttgc atatcggtat gttccgaaaa aggaatattg tgttttgtaa gaatttgttt
     1621 gtattcattt ggcaatgtaa gttccggtgg cgaaacaaag taaaatcgag gattgtagtt
     1681 catcattgcc ataataagcg aatgtacggt tcgtccatat ttaaggtcgc caataagagt
     1741 aattgcaagg ttttgcaacg tgccttgtgt tttaagtatg gaatacatgt caagcaaggt
     1801 ttgtgttggg tgttgatttg ctccgtcgcc ggcatttata ataggtaccg atgatacttc
     1861 acttgcatat cgggctgcac cttctagtgg gtggcgcatt acaataaggt cgcaatatcc
     1921 tgataccatt cgaatagaat catgtaatga ttcacctttt gtagaactgg tggtgccaat
     1981 atctgcaaat ccaataacac gtccgcgtag gcggtttata gcactttcaa aacttaatcg
     2041 tgtacgggta gagggttcaa agaatagggt tgcaataact cttccgtgta aaagattttg
     2101 ataactgttt gcttcaaatt ctgaagctaa ttgtaaaatt cggttgtaat cttcgacttt
     2161 atagtctgat attgatacta aactttttgc tgtcataatg ccgcaaatat aaaaaaagct
     2221 gattgtgttt caatcagctt ttttaaaaaa tattattctt tttcgtgcaa atcttcaaat
     2281 tctatattct tatactcgtc ttgtaattct aatgaatgta attcgctctg tttattaagt
     2341 tctacaaacc taaatgcatc atttaaacct tccataaatt tttcaaaatc ttcgcggtac
     2401 aaaaagattt tgtgtttttc ataactaaat tgtccttcag attgaaacac acgtttgctt
     2461 tctattatag ttaaaaagaa atcgtttgtt ttggttactt taatatcaaa aaaataggtg
     2521 cgtttgccgg cacgaataga ttttgaataa gcgtcttcct taaattcatt gtgtaattgt
     2581 tcactcatag tttttcattt ttatattctt ccttgttcaa atataggata tttaatttgt
     2641 aaaaaaaaat attttcacaa attcttttct atgaaacttc attttgtttt aaatacacta
     2701 aggttgatgt ttcaaaatct aacatttttc ttttaaaata tgcagataac atgtgttgta
     2761 taattatctt ttgtatattt gatagttgta tactatgaat atactgtgtt tgtaagaaat
     2821 cataaacgta tattatgatt acagtgaggt gtgttgtaat atccatgaaa taacgtataa
     2881 agtagatgaa aaaaatagta ataattatgg taatttccac aatgagtttt gcttgttcgc
     2941 aaaaatcaaa aagtattgat gagaaagtaa atgaattgct ttcgcgtatg acgttagatg
     3001 agaagatagg acagttggtt caatacaatg gtttttggga tgcaacaggt cctcttccac
     3061 aagaatctaa ttctaaaagg aagtataacg atgtaaggca aggtttggtt ggttcagtat
     3121 taaatgtata tggtgtagaa caggttcgta agttacaaga aattgctgtt acacaatcgc
     3181 gattgggtat accgcttata tttggaatgg acgtgattca tgggtataaa actgcgtttc
     3241 ctgttcctat agccgaagca gcaagttgga atatgcaaac catagaacaa gctgcacgta
     3301 tatcggcaac tgaagcatcg gcagccggaa ttaattggac atttgctcct atgcttgatg
     3361 tatcgcgtga tccgcggtgg ggacgggtaa tggaaggagc tggagaagac ccttatttga
     3421 ctgcacaggt aggaatagct aaaattaaag gatttcaagg tacagattta tcgcatagtc
     3481 aaactatagc tgcatgtgcc aaacattttg ccggatatgg ttttccaata gcaggacgtg
     3541 attataatac agtagatgta agtaaggtta cactatacaa tgatatattg cctccgttta
     3601 aagctgctgt agaaaataat gtggcaacag ttatgaatgc ttttaatgta ttgaatggtg
     3661 taccttgtac cggtaattca tggttggttc gcgatgtttt aaaaaataca tggaatttta
     3721 aaggatttgt ggtttctgac tgggggtcaa tcatggaaat ggcaacacat ggatatgctc
     3781 aggaccgata tcatgctgca caattagcta tagaagcagg ttgcgatatg gatatggaat
     3841 cgtatgctta tagaaatgat ttaaaaacac ttattgaaaa aggagtagta aaagaatcat
     3901 taattgacga tgctgtgcga agaatattgc gtataaaatt taaaatggga ttgtttgaaa
     3961 atccctataa atattgtgac aaagaacatg aaaaagtaat attgcaaaat acagattatt
     4021 caaaagaagc attgcaaagt gcattagaag ctattgtgtt gttaaaaaat gaacaatcgc
     4081 tgttgccgtt atcaaaaaat caaaaaaata ttgctgtaat aggagctttg gcaaatgata
     4141 aagatactcc gttaggaaat tggagagctc aagctgttaa aaacactgca gtttcggtac
     4201 tcgaaggtat atctgcatat actaatgatt atgtatatgc tcaaggagct ttagtgcata
     4261 ccaatgctgc cggatttact catactgtaa atataaatac aacagataaa agtggatttg
     4321 ctcaagcaat agcagttgca aaaaaagccg atgtaataat tatggtttta ggtgaaaatt
     4381 gttttcaatc gggcgaagca cgaagccgtt ctgatatagg attacccgga gtacaacaag
     4441 aattacttga ggctgtgtat gcagtaaata gaaatatagt tttggtactt atgaacggac
     4501 gacctcttac tatatcatgg gcaggagaac atataccggc aatagtggaa gca
//
LOCUS       DAOTOW010000083         4530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15643_length_4530_cov_3.789721, whole genome shotgun sequence.
ACCESSION   DAOTOW010000083 DAOTOW010000000
VERSION     DAOTOW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15643_length_4530_cov_3.789721"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..739)
                     /locus_tag="P5243_02145"
     CDS             complement(65..739)
                     /locus_tag="P5243_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18279.1"
                     /translation="MRRYLLVILFFFSLVNLNAQISHLFFVSGGVSIATDVIITDSKF
                     QDMDGYSLNYQHLQWNYTTLMLDGRMNLFTIGNSSSVSISTIPSVGFGMAYEIYDGGT
                     WYSLGAGVPAFLEYNFGNASQYNALQNFGFVVGLGYEYHIYPLIAYPVSETHLNVSEF
                     RKIWSQPVFELGLRYYNKKNRAKEVNFKLGYGDKGKDFIIFGGNTENAKQTMSFRVAF
                     VRFFNY"
     gene            1128..2537
                     /locus_tag="P5243_02150"
     CDS             1128..2537
                     /locus_tag="P5243_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRS18280.1"
                     /translation="MKKFEITTYSKTILADTLTPVNVYLKIRDKFSNPLLLESTDFNG
                     DENSYSIVCFEPIAYIQVQNSILTKQYPDGTSELLEIPKSKYFVPQQMHDFFQSFSIS
                     KATVPLRVEGFFGYNSYDAVKYFETIELHTPTKKENEIPEIFFNFYKYIVAFNHFKSE
                     MHIIENVFENQPSTLSEIESIILNKNFCTYSFNTVGKESSNFTDSEFIEVIKNGKKHC
                     FRGDVIQIVLSRQFSQAFTGDEFNVYRTLRSINPSPYLFFFDFGTFKIFGSSPESQIV
                     IRDNKATINPIAGTFRRTGDDIKDAELAKQLAADPKENSEHIMLVDLARNDLSRNGHN
                     VTVEKYREIQYFSHVLHMVSKVSAQLNSQTNPIQLIADTFPAGTLSGAPKYRAMELID
                     TYENQGRGFYGGSLGYIDIHGNVNQAIMIRSFLSKDNILYYQAGAGVVAKSIEESELQ
                     EINNKLAALKSAIHKAEQL"
     gene            2548..3117
                     /locus_tag="P5243_02155"
     CDS             2548..3117
                     /locus_tag="P5243_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRS18281.1"
                     /translation="MAKILVLDNYDSFTYNLIHYIKELTTDTVDVFRNNEISIDAVAM
                     YDKILLSPGPGIPSEAGIMPQVIKQYGATKNILGVCLGHQAIVEAYGGEIYNMPTVYH
                     GVESLLTIIGNDELYSGFNKTFLAGRYHSWNAVKNTLPDCFIITAVDELGEVMSVKHK
                     THNVRGVQYHPESVLTPEGKKLIQNWLAI"
     gene            3132..4121
                     /gene="trpD"
                     /locus_tag="P5243_02160"
     CDS             3132..4121
                     /gene="trpD"
                     /locus_tag="P5243_02160"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRS18282.1"
                     /translation="MKEILQYLYTYQTLSKEQAKEIIHNIREKKYNEIQIASFLTVFS
                     MRSITIDEMNGFVEALKESCISVNLQGYDTIDMCGTGGDGKNTFNISTIASFIVAGAG
                     INVAKHGNYGVSSGCGSSNLMEALGYTFSTDSAKLQSDIEKAGMCYMHAPLFHPTMKE
                     VAPIRKQLIVRTFFNMAGPLVNPSNPTAQSVGVYNLEVGRIYKYLFQKNNKQFIIVHG
                     LDGYDEISLTGQVKLMSNTFERIVTPYDFGFKPLPPSQIDGGNTIDDAVKICMQILTN
                     KATQAQINVVLANAAIGIKSIKIHQSIEECLAQARESLESGKALTVLKKLIQK"
     gene            complement(4127..>4530)
                     /locus_tag="P5243_02165"
     CDS             complement(4127..>4530)
                     /locus_tag="P5243_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18283.1"
                     /translation="QPIVFEQIKMYNSLIVAYCKQLQTIKSKLQNGIGEDTLYITAVK
                     EYTEYMERVSNEFVKAAIALPEISKILHEHIELLQECIKHFKKDNELEVSRHTQYMEY
                     RKKFRQIDTEYIKHFQAVNKEYRKIIQKALK"
BASE COUNT         1673 a          692 c          635 g         1530 t
ORIGIN      
        1 acacttacaa aaagtaggag cgacagtaat cgtttcatag tttgtatatt taatatatgt
       61 atactcaata gttaaaaaat cttacaaatg caactctaaa actcattgtt tgttttgcat
      121 tttcagtatt accaccaaaa ataataaaat cttttccttt atcaccatat ccgagcttaa
      181 aatttacttc ttttgcccta ttttttttat tataataccg taatcctaat tcaaaaaccg
      241 gttgtgacca tatttttctg aattccgata catttaaatg agtctcagaa acaggatatg
      301 caattaatgg atatatatga tattcatatc ctaaaccaac tacaaaacca aaattttgta
      361 atgcattata ttgtgacgca tttccaaaat tatattccaa aaatgctggt acacccgctc
      421 ctaaactata ccaagttccg ccatcatata tttcatacgc cataccaaac ccaacagaag
      481 gaattgtaga gattgaaact gacgaactat ttccaatagt aaaaagattc attctgccat
      541 ctaacataag agttgtataa ttccattgta aatgttgata atttaatgaa tacccatcca
      601 tatcttgaaa tttactatca gttattatta catcagttgc tatactaact ccacctgaca
      661 cgaaaaataa atgcgaaatt tgtgcattaa gattcactaa actaaaaaaa aatagtatta
      721 ccaataaata tcgccgcata gtattcgtat ctactataca aatatactgt tattttattg
      781 taaaaaacaa gtgtatgcaa atgtttttca tttaaacatg ttactgtttt agaaaattaa
      841 tacgtaataa aatacaattt ataacaattc aaaattaata ataattatat aaatcattaa
      901 tattaaacat gttatatatt ttttagataa aaaaaactta gaacattgat ttttattaaa
      961 aattttataa tatttgcata aataagtaaa atatgaaaca acaattacat atatggtggt
     1021 ggcgtaaaca aattattcat ttgtgagata gcttattcca tattgtaata taactataca
     1081 aaagcttgcc tcacacgagg caagcttttt tttttaatat atcagttatg aaaaaatttg
     1141 aaattacaac ctattcaaaa accattttag ccgacacact aactccggta aatgtgtatt
     1201 taaaaattcg agataaattt tcaaacccat tacttctgga aagtactgat tttaatggag
     1261 atgaaaacag ttattctatt gtatgttttg aacctatagc ctatatacaa gttcaaaatt
     1321 caattcttac aaaacaatat cctgatggta cttcagaact attagaaatt ccaaaatcaa
     1381 aatattttgt tcctcaacaa atgcatgatt tttttcaatc gtttagtatt tctaaagcta
     1441 cagtacctct gcgagttgaa ggtttttttg gttacaattc atatgatgca gtaaaatatt
     1501 ttgaaacaat agaattgcat accccaacta aaaaagaaaa tgaaattccc gagatttttt
     1561 ttaattttta taaatatatc gttgcattta accattttaa aagcgaaatg catattatag
     1621 aaaacgtttt tgaaaatcaa ccaagcactc tatcagaaat tgaatcaatt attttaaata
     1681 aaaatttttg tacatattca tttaatacgg taggaaaaga atcgtcaaat tttacagatt
     1741 ctgaatttat agaggttatt aaaaatggta aaaaacattg tttcagaggt gacgttattc
     1801 aaattgttct ttctcgtcaa ttttctcaag catttaccgg cgacgaattt aacgtatata
     1861 gaactcttcg ttcaataaat ccctctccat acttattctt ttttgatttc ggcacattta
     1921 aaatttttgg ttcgtcacct gaatctcaaa ttgtaataag agataataaa gcaactataa
     1981 acccaattgc aggtactttt cgtagaaccg gcgacgatat aaaagatgcc gaactagcaa
     2041 aacaattagc tgctgaccca aaagaaaatt cagaacatat tatgttagtt gatttagctc
     2101 gaaacgattt aagtagaaac ggacataatg taactgttga aaaatataga gaaatacaat
     2161 atttttcgca tgtattacat atggtttcaa aagtatcggc acaattaaat tctcaaacca
     2221 atccaattca acttattgca gatacttttc cggcaggaac cctttccggt gctcctaaat
     2281 acagagctat ggaattgatt gacacttacg aaaatcaagg tcgtggattt tatggaggca
     2341 gtttaggata tattgacata catggcaatg taaatcaagc tattatgata cgttcatttt
     2401 taagtaaaga taatatatta tactatcaag caggcgcagg tgttgttgct aaatcaattg
     2461 aagaaagtga acttcaagaa attaacaata aattagcggc attaaaaagt gctattcaca
     2521 aagcagaaca attataaaaa tataattatg gcaaaaatat tagttctaga caattacgat
     2581 tcttttacat ataatcttat acattatata aaagaattaa caacagatac cgtagatgtt
     2641 tttcggaata acgaaatttc aatagacgca gtagcaatgt atgataaaat attattatct
     2701 cctgggccgg gtataccatc tgaagcagga ataatgcctc aagttataaa acaatatggt
     2761 gctaccaaaa atatactcgg agtttgttta ggtcatcaag ctatagttga agcatatggt
     2821 ggtgaaattt ataatatgcc taccgtttat catggcgttg aatctttact tactataatt
     2881 ggtaatgatg aattatattc cggttttaac aaaacctttt tagccggcag atatcattca
     2941 tggaatgcag taaaaaatac attacctgat tgttttatta taactgctgt tgacgaatta
     3001 ggcgaagtaa tgagtgtaaa acacaaaact cataatgtaa gaggtgttca atatcatcct
     3061 gaatcggtat taacacctga aggtaaaaaa ttaatccaaa attggttagc aatttaaatt
     3121 tgtaataagc aatgaaagaa attcttcaat atttatatac atatcaaact ctatcaaaag
     3181 aacaagctaa agaaataatt cataatatac gtgagaaaaa atataatgaa attcaaatag
     3241 cttccttttt aacggtattt tcaatgcgtt caataacaat tgatgaaatg aatggatttg
     3301 ttgaagcatt gaaagaatca tgtatttcgg taaatttaca gggctatgat accatagaca
     3361 tgtgtggtac cggcggcgat ggaaaaaaca catttaacat ttcaactatt gcatcattta
     3421 tagttgccgg cgccggtatt aatgtagcaa aacacggaaa ttatggggtg tcatcaggat
     3481 gtggttcttc aaatttaatg gaggctttag gatatacttt ttcaactgat tcggctaaat
     3541 tacaaagcga tatagaaaaa gcaggcatgt gctatatgca tgctccatta tttcacccga
     3601 ctatgaaaga agtagctcct attcgaaaac aactcatagt gagaacattt tttaacatgg
     3661 caggtccgtt agttaatcct tcaaatccta cagctcagtc ggtaggtgta tataatttag
     3721 aagtaggtag aatttataaa tatctttttc agaaaaataa caaacaattc attatagttc
     3781 atggactcga cggatacgat gaaatttcgt taaccggaca ggtaaaatta atgtcaaata
     3841 catttgaacg aattgtaacc ccctatgatt ttggatttaa acctttacca ccgtctcaaa
     3901 ttgatggcgg taataccatt gatgatgctg taaaaatttg tatgcaaatt ttaacaaaca
     3961 aagcaactca agcacaaata aatgttgttt tagcaaatgc tgcaattgga attaaaagta
     4021 ttaaaataca tcaaagtatt gaagaatgtt tagctcaagc tcgagaatca ttagaaagcg
     4081 gtaaagctct tactgtattg aaaaaactta tacaaaaata atataactat tttaatgcct
     4141 tttgaattat tttacgatat tctttattta ccgcttgaaa atgcttaata tattcagtat
     4201 ctatttgtct aaatttttta cgatattcca tatattgagt atgtctgctt acttctaatt
     4261 cattatcttt tttgaaatgc ttaatacatt cttgcaataa ttctatatgt tcatgaagaa
     4321 tttttgaaat ttcaggaaga gcaatagcgg cttttacaaa ctcgtttgaa actcgttcca
     4381 tatactcggt atattctttt actgctgtta tgtataaagt atcttcacct attccatttt
     4441 gcaattttga tttaattgtc tgcaattgtt tacaatacgc cacaattaaa ctattataca
     4501 tcttaatttg ttcaaaaact attggttgct
//
LOCUS       DAOTOW010000084         4520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15688_length_4520_cov_3.286226, whole genome shotgun sequence.
ACCESSION   DAOTOW010000084 DAOTOW010000000
VERSION     DAOTOW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15688_length_4520_cov_3.286226"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..491)
                     /locus_tag="P5243_02170"
     CDS             complement(15..491)
                     /locus_tag="P5243_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS18284.1"
                     /translation="MAKIRKGGKKVAPAISTASLPDIVFMLLFFFMVSTTMRESTLLV
                     KKPRLPKGTELTKLEKKSLVSNIYVAQPTVELQAVFGNQPVIQLNESIEDVSKIGRFV
                     QLEREARNPNDRDFLTMNLKVDSKTKMGIITDIKMELRKANALKLNYSALPRGVKH"
     gene            complement(494..1081)
                     /locus_tag="P5243_02175"
     CDS             complement(494..1081)
                     /locus_tag="P5243_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS18285.1"
                     /translation="MARRSLPEINSASMADIAFMLLIFFLVATTMDSDKGIFRVLPPY
                     IDKPVQDDPIKVLDKNLFVVLINKDDRLMVEKKPMHPDELRKACKDFLINMNNDENDK
                     VYPEYKEETIAGLGTVKVTKGIVSIQNDRGTSYDKYIEIQNEIVAAYNEVKDEYSIKY
                     FRKPFKELNEDQQKAIKLLIPQRISEAEPKNIGGK"
     gene            complement(1136..1750)
                     /locus_tag="P5243_02180"
     CDS             complement(1136..1750)
                     /locus_tag="P5243_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18286.1"
                     /translation="MNFNKLFKIVSISLFAIAIIASVMYIGAASKKNCFACKLPVCEK
                     VDAKGEKYLAPADECAMTYNEIKKMEQETMQRNKDFGTNDPVEISECSKECLNYAGNF
                     MMFTIVITIVAIVLALLFSVYMLIVDTKKIKGVLIGVGSIGLITLISYLMSSDSIPSI
                     LGYSNVITIGEVKMVDTMLFMTYILLAISIISILYTSIVKIIKK"
     gene            complement(1829..2626)
                     /locus_tag="P5243_02185"
     CDS             complement(1829..2626)
                     /locus_tag="P5243_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRS18287.1"
                     /translation="MKKLFALLAVIGLLSVTGFSQETKQEVTTKDSVTAVDSTKTAAA
                     AEENAGPVQEKGLHQVIKEKFIEGGAGFMAFVVLCLILGLAIAIERIIYLNLATTNTE
                     KLLKKVEDALQSGGKEAALEVCRNTPGPVASIFYQGISRSDEGAEIVEKTVVSYGSVQ
                     MGLLEKGLTWLSLFIALAPMLGFMGTVIGMIEAFDAIAIAGDISPALVAGGIKVALIT
                     TVGGLIVAMILQVFYNYLLSKVDALTTDMEDASISLVDILVKYNAKK"
     gene            complement(2697..2786)
                     /locus_tag="P5243_02190"
     tRNA            complement(2697..2786)
                     /locus_tag="P5243_02190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2750..2752),aa:Ser,seq:gga)
     gene            2908..3402
                     /locus_tag="P5243_02195"
     CDS             2908..3402
                     /locus_tag="P5243_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRS18288.1"
                     /translation="MNSKKIVSDFLNLPKPINDADISASLTQIKKKAPKNLDAIVHKL
                     HRETFETINCLDCANCCKTLSPGITDTDIQRLSKHLKLKPSQLVEQYFTLDNDGLYMF
                     TQSPCPFLMPDNYCSVYEYRPRACREYPHTDRRRFEQLTRITKENVSVCPAVYHILKK
                     LHYT"
     gene            3452..4279
                     /locus_tag="P5243_02200"
     CDS             3452..4279
                     /locus_tag="P5243_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS18289.1"
                     /translation="MNVRTVLQTFTTRFALLVLNFGIIIFTTNMWGSEGKGIISLLIA
                     DLAIVCFASNILVGSSISYFSTRLPLQQLIILSYIWSLLISITIPFIVTTSHSQEYVW
                     YLIIISFVFSLLTANTNIFIGQNNLSRFNLYTILQQLVLIVFLIIYIYVFSWTDVYVY
                     FIAQICAYTLLFITSSIEIFKPLQFKNVTISKTSFKSLFSYGWKTQLYSFLHFLSNRL
                     SYYFLEALQGIASVGVFSVGVAFSEAIWTVSRSLAVILYSDIISQKQDISYIQQTKL"
BASE COUNT         1446 a          781 c          571 g         1722 t
ORIGIN      
        1 ttcttactat tttattaatg ttttactcca cgaggcaatg cactataatt taatttaaga
       61 gcatttgctt tacgcaattc cattttaata tctgttataa ttcccatttt tgttttagaa
      121 tcaactttta gattcatagt caaaaaatct ctatcgttag gatttcgtgc ctcacgttcc
      181 aattgaacaa aacgaccaat ttttgataca tcttcaattg attcatttaa ctgaattaca
      241 ggttgattac caaaaactgc ttgtaattca acagttggtt gtgctacata aatattacta
      301 accaatgatt ttttttctaa ttttgttaat tcggttcctt ttggtaatcg aggtttttta
      361 actaacaatg tactttcacg catagttgta ctaaccataa agaaaaataa caacataaat
      421 acaatatcag gtaatgatgc tgttgaaatt gctggagcaa ccttttttcc accttttcta
      481 atttttgcca tgtttatttt cctccaatat ttttaggttc tgcttcagaa atacgttgcg
      541 gaatcaataa ttttatagcc ttttgttggt cttcattcaa ttctttgaat ggtttacgaa
      601 aatattttat agaatactcg tctttaacct cattgtaagc cgcaactatt tcattttgaa
      661 tctcaatata tttatcgtat gatgtaccac ggtcattttg aatacttaca atacctttag
      721 taacttttac agttcccaat ccggctattg tttcttcttt atattcagga taaaccttat
      781 cattttcgtc attattcata ttgattaaaa aatccttgca tgcttttcgt aattcatcag
      841 gatgcatagg ttttttttca accataagtc tatcatcttt attgataagc acaacgaata
      901 agtttttatc taataccttg atagggtcgt cttgaacggg tttatctata tatggtggca
      961 acacacgaaa gataccttta tcagaatcca tagttgttgc tactaagaag aaaattaaaa
     1021 gcataaatgc tatatcggcc atagatgctg aattaatttc cggtaatgat cttcgagcca
     1081 taatgtttta aattttagat atctaacgta ctattctcaa ccgttacgtt actatttatt
     1141 ttttaataat ttttacaata cttgtataga gaatactaat aattgatatt gctaataata
     1201 tatatgtcat aaacaacata gtatcaacca tttttacttc acctatagta ataacatttg
     1261 aataccctaa aattgaagga attgaatctg atgacattag gtaagaaata agagttataa
     1321 gacctatact accaacacct atcaatacac ctttaatttt tttagtatca actataagca
     1381 tatatactga aaataaaaga gctaatacta tagcaactat agtaattaca atagtaaaca
     1441 tcataaagtt accggcataa tttaaacatt ctttcgaaca ttctgaaatt tcaaccggat
     1501 cgtttgttcc aaaatcttta ttacgctgca tagtttcttg ctccattttc ttaatttcat
     1561 tgtaagtcat ggcgcattca tcggcaggtg ctaaatattt ttcgcctttt gcatctactt
     1621 tttcacatac cggcaactta catgcaaaac aattcttttt agaagctgct cctatataca
     1681 ttacacttgc tattattgca atagcaaata atgaaataga cactatttta aataatttat
     1741 tgaaattcat cggaatatat tttttttagt ttatacgata gtgtatatat acaaaccggt
     1801 tcaatatgta ttgaaccggt tattatattt attttttcgc attatatttt actaatatat
     1861 ctacaagtga aatagaagca tcttccatat cagtagttaa tgcatcaact ttagataata
     1921 aatagttata gaatacttga agaatcatag ctactatcaa accaccaaca gttgtaataa
     1981 gtgctacttt aataccacct gcaactaatg ccggagaaat atcacctgct atagcaatag
     2041 catcaaacgc ttcaatcata ccaattacag tacccatgaa ccctaacata ggtgccaatg
     2101 caataaataa tgataaccaa gttaatcctt tttcaagtaa tcccatttga acacttccgt
     2161 atgaaacaac tgttttttca actatttcag caccttcgtc gcttcggctt ataccttgat
     2221 aaaatatact tgccacagga ccgggagtat ttctgcaaac ctctaaagct gcttcttttc
     2281 ctcctgattg taatgcatct tcaacttttt tcaataattt ttctgtattt gttgttgcaa
     2341 gattaaggta aataatacgc tctatagcta ttgcaagacc taaaatcaaa cataaaacca
     2401 caaatgccat aaatccggca ccaccttcaa taaatttttc tttaattact tggtgtaatc
     2461 ctttttcttg aacaggtcct gcattttctt ctgccgcagc agctgttttt gttgaatcaa
     2521 ccgcagttac agaatctttg gtggtaactt cttgttttgt ttcttgtgaa aaaccggtta
     2581 cgcttaaaag tccaataacc gctaaaagtg caaataactt tttcataatg tactgttatt
     2641 ttaattaatg tttagttttt aatttatatt ctattttatt attttctaat cttacgcgga
     2701 gagagaggga ttcgaaccct cgatgccgtt tcaggcggca tacacacttt ccaggcgtgc
     2761 tccttcaacc actcggacac ctctcccttc cactgccaaa aatacacaaa aattgaatat
     2821 catatatttt ttcatgataa atttttatta aattttaaat ttattcaaat accgctactt
     2881 ttgcatatat ttttttatgc aaagcgtatg aattccaaaa aaattgtgtc agattttttg
     2941 aatcttccaa aacctattaa cgatgctgat atttcagctt ctttaacaca aatcaaaaaa
     3001 aaagctccaa aaaatttaga cgcaattgta cacaaattac acagagaaac atttgaaact
     3061 attaattgtt tagattgtgc taattgttgt aaaactttga gtcccggaat taccgatacc
     3121 gatattcaac gattatctaa acatctaaaa ttaaaaccat cacaattagt tgaacaatat
     3181 tttactttag acaatgatgg gttatatatg tttactcaaa gcccttgtcc ttttttaatg
     3241 cctgataatt attgttcggt ttatgaatat cgtcctcgtg cttgtcgcga atatccccat
     3301 accgaccgcc gccgatttga acaacttacc cgaattacaa aagaaaatgt ttcagtttgt
     3361 cctgctgttt atcatatttt gaaaaaacta cattatacat aattataatt aggaataata
     3421 tttacttttg tttatctatt ttaatttgta tatgaacgtt cgtacagttt tacaaacctt
     3481 tacaactcgt tttgctttat tagtactcaa tttcggtatc attattttta ctacaaatat
     3541 gtggggtagc gaaggtaaag gtattatttc cttattaatt gctgatttag ctatagtctg
     3601 ttttgccagt aatattttag ttggcagcag catatcatat ttttctacac gactcccatt
     3661 acaacaactt ataatattat catacatttg gtcattactt ataagcataa ccattccttt
     3721 tatcgttacc acatcgcact cgcaagaata tgtttggtat ttaattatca tttcatttgt
     3781 tttttcatta cttactgcaa atacaaatat tttcataggt caaaataatt tatctcgttt
     3841 taatttatat accatacttc aacaactcgt tttaattgtt tttttgatta tatatatata
     3901 tgtattttca tggactgatg tgtatgtata ttttattgct caaatttgtg catatacttt
     3961 attatttatt accagcagta tagaaatttt taaaccatta caatttaaaa atgtaaccat
     4021 atcaaaaaca tcttttaaat cgctgttttc gtacggttgg aaaactcaac tgtattcttt
     4081 cttacatttt ttgagcaatc gtctttcata ctatttttta gaagctttac aaggaattgc
     4141 gagtgttggt gtattttctg taggcgttgc tttttcagaa gctatttgga cagtaagtag
     4201 aagtttggct gtaatattat attccgacat aatttctcaa aaacaagata tttcttacat
     4261 tcaacaaaca aaattataat gaaaaatttg ttttgtttta acctgctatt ttttccttat
     4321 tatgatattt tttccaaatt cattctacat tattgttttt gggaaagact ttgctcaaac
     4381 caaaaatatt attatgcttc tttctccggg aatacttgca atagctattt ctaatattat
     4441 tggtcattat ttctcggcat taggaatttt acgaatttta aacataaaag cgtttattgg
     4501 attaggaatt acaataattg
//
LOCUS       DAOTOW010000085         4494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15798_length_4494_cov_3.645190, whole genome shotgun sequence.
ACCESSION   DAOTOW010000085 DAOTOW010000000
VERSION     DAOTOW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15798_length_4494_cov_3.645190"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="P5243_02205"
     CDS             complement(<1..1022)
                     /locus_tag="P5243_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18290.1"
                     /translation="MKTTRIITVLLTILSLVVTDLFAQKDLVISGGNTVSSFVCSNRK
                     VYTWGNNKTTTGNGLLGCVSCTADMYTSPQPVNFPGGVDITQVNSGSGSHFLALDCNG
                     NPWAWGNNSLGQVGNGSAAGSGGFVGTPTRVLALPEIAATHKNASNQLINVKVVYAGN
                     NSSFAILDNGDLVSWGANGKGFDAMGYDDAYGQLGNGVLPAVGTTANQTSAGYVKVPG
                     GGKLTGVTQVFAGDNFAMALVDPDGDGIGTVYTWGDGKSGTLARNAAGTLNPSNGGEE
                     KDPWARPARYADGTIMNNITQIYAGDVFGAALDVNGYIWTWGNGGWNNSTGNTTVNYT
                     GSDPRRV"
     assembly_gap    1452..1551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1874..2323)
                     /locus_tag="P5243_02210"
     CDS             complement(1874..2323)
                     /locus_tag="P5243_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRS18291.1"
                     /translation="MKLTKQIATDLMAAMKQQEKEKLEALRAIKTAFIIAKSNIGADA
                     ELSDDEELKIIQKLVKQRKDSAAEYTAQNRTDLAQKELAEAEVISAYLPQQMSEDELR
                     QEIAAIIKETGAVGMKDMGKVMGVANKKLAGKADGKAISEVVKALLG"
     gene            complement(2353..3135)
                     /gene="mazG"
                     /locus_tag="P5243_02215"
     CDS             complement(2353..3135)
                     /gene="mazG"
                     /locus_tag="P5243_02215"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRS18292.1"
                     /translation="MQTTLESFSRLLHIMDELREKCPWDKKQTFETLRNLTIEEVYEL
                     ADAIIQQNSQEIKKELGDLLLHIVFYAKIASETNEFNMKDVIDSLCEKLIYRHPHVFG
                     EESLQNQEQVKERWEQLKLKEKDRPKTVLGGVPETMPALLKAMRMQQKARAVGFDWEY
                     KEQVWDKVQEEISELSHEIQTSQNPEKIEQEFGDVLFSIINAARLYGVDPETALERTN
                     KKFKFRFEYLESQTMAKGISLKDLSLEQMDVFWNEAKKIEKK"
     gene            complement(3146..3991)
                     /locus_tag="P5243_02220"
     CDS             complement(3146..3991)
                     /locus_tag="P5243_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRS18293.1"
                     /translation="MITVTLTTDWGADGLYTGAFKGKLVSRVPGVQLVDITHQVDALS
                     PLKAVFALQHAYQYFMKKTIHIVGVGGKISPGSELEVPEYICFQYNHHYFIGPNNGIW
                     EALFGEVPKEVFVLERSPLSKKYYAFPELDIFIDAVGKLAMGMEPKHLGESVPCKLGR
                     RIGLPVKKHNQLLGEFLYFDVYGNGITNISKDEFYEVCNNRPFTIMVGMQRKQYITDV
                     IAEDYNQGDSSRIVALFSFTGYLEIAVPQKQLTQFVHIDKNTKVLIQFYDSIEEKQQD
                     EGVLL"
     gene            4071..>4494
                     /locus_tag="P5243_02225"
     CDS             4071..>4494
                     /locus_tag="P5243_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRS18294.1"
                     /translation="MLEHIISLQSIDPIDVYGVKNEKIDAVKKAFPKLKIIARGSEIK
                     IIGNKKEIEIFELQFEHILYHIRKHHGISLSHIQDILSSYEHNESLFDNENPNNDVLF
                     FGNGGKPIKARTPNQKKLVKKEGQSDLLFAIGPAGTGKT"
BASE COUNT         1380 a          865 c          629 g         1520 t
ORIGIN      
        1 actcttcgtg gatcagaacc ggtatagttt accgtagtat tacctgttga gttattccaa
       61 ccaccattcc cccatgtcca aatatatcca tttacatcaa gtgcagctcc aaatacgtcg
      121 ccggcataaa tttgtgtaat attattcatt atggtaccat cggcatagcg cgctggccga
      181 gcccatgggt ctttttcttc accaccgttt gatggattta atgtgcctgc ggcatttcgt
      241 gccaacgtac cacttttacc atcaccccaa gtatatacag ttcctatacc atcaccatca
      301 gggtcaacca atgccatagc aaagttgtca cctgcaaata cctgagtaac acctgttaat
      361 ttaccaccac ccggtacttt aacatagcct gcagatgttt gatttgctgt tgttccaact
      421 gcaggcaaca ctccgtttcc taattgacca taggcatcat cataccccat agcatcaaac
      481 ccttttccat ttgcccccca tgacactaaa tcaccattat ctaatatagc aaaacttgag
      541 ttattaccgg catatacaac tttaacattt attaattggt ttgaagcatt tttatgcgtt
      601 gcagctattt caggtaatgc caaaacacga gtaggagttc ctacaaatcc acctgaaccg
      661 gcagcagaac cattaccaac ttgccccaaa ctattattac cccatgccca tggatttcca
      721 ttacaatcca aagccaaaaa gtgagaaccc gaacccgagt taacttgggt aatatctaca
      781 cctccgggaa aatttaccgg ttgaggcgat gtatacatat cggcagtaca actcacacaa
      841 cctaataaac cgttaccggt tgtggtttta ttgttacccc aggtatacac ttttcggttc
      901 gaacacacga aacttgatac cgtattacct ccggaaatta ccagatcttt ctgtgcaaac
      961 aaatctgtca caactaatga caaaatcgta agcaatactg taataattct tgttgttttc
     1021 atagctatat tattaaaagg tggttactca tatgtatttt ttactatata caataattcc
     1081 atttacatta taagacaaaa ctttatacaa aaaggttgca tacattataa aaaaaagata
     1141 cattttttta cgtaattgct gctaagcctt attactttag tattttgttg ttataaatta
     1201 tacaatatta ttctccgcaa ttttttattt ttttgaggat ttccctccaa gattgcaaaa
     1261 attaaaagaa aaaatgaaaa atgcaagttt tttgcaaaaa atattgagtt aagatagttt
     1321 ataaagttag aaagttttta aaaaatatac cccaatgcat attaggaagt cctccctcaa
     1381 aggatgtgtc tcgccctggc ggacggaggg tgatattata attcttaatt atgaattatt
     1441 aatttttaat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctcaaggga
     1561 ggtgtcttgc cttggcggac ggagggtgat attataattc ttaattatga attattaatt
     1621 tttaattatt ttgaatactg gaataatcca acttaaaata ttcaccccta tcgtcccctc
     1681 aaggggacag tttcaaataa agccttgaat tatacccaaa actatatttt aaaagtcctc
     1741 cctcaaggga ggtgtcttgc cttggcggac ggagggtgat attataattc ttaattttta
     1801 attggtatca atgctatata tacaaaaact taaaactaag aactaaaaac taaaaactaa
     1861 aaaatctaga cagttacccc aacaacgctt ttaccacttc cgaaatagct ttaccatcgg
     1921 ctttacctgc taatttttta tttgcaactc ccattacttt gcccatgtct ttcataccta
     1981 cagctccggt ttcctttatt attgcagcaa tttcttgtcg taattcgtct tcagacattt
     2041 gttgtggcaa atatgcagat attacttctg cttcggcaag ttctttttgt gccaaatctg
     2101 ttctgttttg tgcggtgtat tctgcagctg aatcttttcg ttgttttact aatttttgaa
     2161 taattttcaa ctcttcgtca tcgcttaatt cagcatcggc acctatatta gattttgcaa
     2221 ttataaaagc tgtttttata gcacgcaatg cttctaattt ttctttttct tgttgtttca
     2281 ttgctgccat caaatctgtg gcaatttgtt ttgtgagttt cattgtgata ttgtttatac
     2341 tattatgtac tattattttt tttctatctt tttggcttcg ttccaaaata catccatttg
     2401 ctccaaactt agatctttga gtgaaattcc ttttgccatt gtttgcgatt ctaaatattc
     2461 gaaacgaaat ttaaactttt tattagttcg ttctaatgca gtttcagggt ctactccata
     2521 taagcgagct gcattgatta ttgaaaataa tacatcacca aattcttgtt caattttttc
     2581 ggggttttga gaagtttgta tttcatgtga taattctgaa atttcttctt gtactttatc
     2641 ccacacttgt tctttgtatt cccagtcgaa gccaactgcg cgtgcttttt gttgcatacg
     2701 catagctttg agtaaagccg gcatagtttc gggcacccct cctaaaactg tctttggacg
     2761 gtctttctcc tttaatttta attgctccca gcgttctttt acttgttctt gattttgtaa
     2821 cgactcttca ccaaaaacat gtggatgtcg atatataagt ttttcacaca aactgtctat
     2881 tacatctttc atgttgaact catttgtttc tgaggctatt tttgcgtaaa aaacaatatg
     2941 taatagtaaa tctcctaatt ctttttttat ttcttgtgag ttttgttgaa taattgcatc
     3001 agccaactca tatacttctt ctatagtaag attccgcaac gtttcaaagg tttgtttttt
     3061 atcccacgga catttttcac gtaattcatc cattatatgt aacaatcgcg agaaagattc
     3121 taatgtagtt tgcataccat ttcaattata ataacactcc ttcatcttgc tgtttttctt
     3181 caattgaatc ataaaactga atcaatactt ttgtattttt atcaatatgt acaaactgtg
     3241 ttaattgctt ttgaggaact gcaatttcta aatatcctgt aaaactaaat aaagcaacaa
     3301 ttctggaact atcaccttga ttataatcct cggctattac atcagtaata tattgtttac
     3361 gttgcattcc taccattata gtaaatggtc ggttattaca aacttcataa aactcatctt
     3421 ttgaaatatt ggtaatgcca tttccataca catcaaaata cagaaattct cccaataatt
     3481 gattatgctt tttaacaggc aatccaattc tacgtcctaa tttacatgga actgattcgc
     3541 ccaaatgttt tggctccatt cccattgcca acttaccaac ggcatcaata aatatgtcta
     3601 attcaggaaa tgcataatat ttttttgata acggacttct ttctaaaaca aaaacctctt
     3661 taggtacttc accaaataac gcttcccaaa ttccattatt aggaccaata aaataatgat
     3721 gattatattg aaaacatata tattcaggaa cttctaattc actcccgggt gaaattttac
     3781 cccctactcc tactatatga atagtctttt tcataaaata ctgataggca tgctgcaatg
     3841 caaatacagc cttaagagga ctaagagcat caacctgatg agttatatca actaattgaa
     3901 caccgggtac acgcgacacc aatttgcctt taaatgctcc ggtatataac ccgtcggctc
     3961 cccaatctgt agttaatgta actgtaatca tagaatttac ctaaatactt acaaaagtag
     4021 aaataaatca gtatttttgc tttcaaatat acaaaatatt tatttacttt atgttagaac
     4081 atataatatc attacaatct attgacccta ttgatgtata tggcgttaaa aatgaaaaaa
     4141 tagatgctgt aaaaaaggca tttcctaaac ttaaaattat agcaagaggc agtgaaatta
     4201 agataattgg caacaaaaaa gaaatagaaa tatttgaatt acaatttgaa catattttat
     4261 atcatatacg caaacatcac ggaatttcgc ttagccatat tcaagatata ttaagttcat
     4321 acgaacacaa tgaatcattg tttgataatg aaaatcccaa taacgatgtt ttatttttcg
     4381 gaaatggagg aaaacctata aaagcacgca cgccaaatca aaaaaaatta gtaaaaaaag
     4441 aaggacaatc agatttacta tttgccatag gcccggcagg aaccggaaaa acat
//
LOCUS       DAOTOW010000086         4492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15807_length_4492_cov_3.901961, whole genome shotgun sequence.
ACCESSION   DAOTOW010000086 DAOTOW010000000
VERSION     DAOTOW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15807_length_4492_cov_3.901961"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..703
                     /locus_tag="P5243_02230"
     CDS             155..703
                     /locus_tag="P5243_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRS18295.1"
                     /translation="MKEKTKEIAVRIKDIREKLGLSIQDVVSKTTINKYDYTDYESGL
                     IDIPVSAVLQIAACFNVDISELLTGEAPRNSGYSITRKGEGVSVERHSQYKYQALAHD
                     FANKKGSPFVLTIDASDEEVHYNHHIGQEFLYVLDGTIKIYFKDDVFSLKEGDSVYFD
                     ANIPHAVKTENGERARVLAVVS"
     gene            856..2085
                     /locus_tag="P5243_02235"
     CDS             856..2085
                     /locus_tag="P5243_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRS18296.1"
                     /translation="MEWWYHDRFGMFIHFGSYSYYGKGEWVMQIEKWSKDAYQTQITS
                     QFNPHNFNAKAIVDVAKSTGMKYIVITAKHHEGFCMWKTNVAGFRDYTGNTVFDLYHY
                     IGFERDILKELKDECDAQGIKFCLYYSILDWNHPSQTLHEYFSVMKNMEAKTDYVLQM
                     KAQLRELITLYKPAVLWFDGDWCENVYPPTLENWWNKDDAINLYNYILSIDSTIIINE
                     RVKRELGLGDFMCPEQKIPDAPLERQWETCQTMNGTWGYDAKSYNAYNSAQSFIHELE
                     LIVSRDGNYLLNIDPLPDGSLNPNMLVILHEIGEWMRVYGESIYGCKRSPFLKQPEWG
                     VYTAKDNILYAHIQKPNEVKTIEIPLNSKKIKSVYEVSYNKQKLQYIKTSQGIMIAVP
                     NKQVYPASIVIAVEYKK"
     gene            complement(2090..3115)
                     /gene="galE"
                     /locus_tag="P5243_02240"
     CDS             complement(2090..3115)
                     /gene="galE"
                     /locus_tag="P5243_02240"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRS18297.1"
                     /translation="MAHILVTGGLGYIGSHTVVELIQHKHSVIVVDNLSNSEQSVLQG
                     IYDITGVLPAFEHIDICNKQELSNCFTKHSPIDAVIHFAAYKAVGESVQKPLEYYTNN
                     VYGLLVLLDVMRAHNCNNLVFSSSCTVYGQPTELPVTEQAPLQKATSPYGNTKQICED
                     IIQDYITSNPLFKASLLRYFNPIGAHPSACIGELPKGIPNNLVPFITQTAAGIRKELQ
                     VFGNDYNTPDGSAIRDYIYVVDLAKAHVASLNRILNNNVNKACEICNLGTGTGVSVLE
                     MIHAFEQATGVTVPYSIAPRRAGDIEQVWASTSYANKLLNWKADTPLIEIMRTAWNWE
                     KKIRGIQ"
     gene            complement(3119..3703)
                     /locus_tag="P5243_02245"
     CDS             complement(3119..3703)
                     /locus_tag="P5243_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HRS18298.1"
                     /translation="MMRISIVLLSCILLFSCEERTISMPKQRGYFRIDFPKREYISTT
                     IPSFPYSFDIPKYSYIQIDTSRNAEPYWLNIQFPTLKATIHISYKNVKHDLSTFVEDA
                     HFLVFKHDIKADAIKTQEFHYNDKQVHGLLFDIKGDAASLMQFYITDSVEHFVRGALY
                     FNVPPNKDSLAPSIQFIREDIIELISSFKWNNKK"
     gene            complement(3705..>4492)
                     /locus_tag="P5243_02250"
     CDS             complement(3705..>4492)
                     /locus_tag="P5243_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRS18299.1"
                     /translation="TLTQLTSQNVMINIAEALHVSSHEVKEEKEIIEGIAKLQQKVVS
                     NIMTPRVSIFALDIATNFKDLVHETTHIGYSRVPVFSGTLDTIKGILYVKDLLPHLEK
                     SQGFRWQTLIRPAYFIPETKKIDNLLEEFQKTKIHLAVVIDEYGGTSGIVTLEDILEE
                     ILGEINDEFDDESDELYTKVNDTTYIFEGKISIHDVCKIIQLPEDFFDSHKGDADSLA
                     GFILELTGDFPKLHQQIIYKHLQFLIEEEDNRRISKIKLNIQE"
BASE COUNT         1522 a          639 c          789 g         1542 t
ORIGIN      
        1 cgagaaaagc catatagtaa aaatttgccg caaggtacaa aaacttataa aaaataaaaa
       61 tcgtgaattg aatgatggag cgaaacaaaa aaaatgtaat tttgtatgaa tcatttttta
      121 tacaatagtt tagaatattt gaaaatttta cggaatgaaa gaaaaaacaa aggaaatagc
      181 agttcgcata aaagatattc gcgaaaaatt gggcttgtca attcaagatg tagtttcaaa
      241 aactactatt aataaatatg attatactga ttacgaaagt ggtctgatag atatacccgt
      301 aagtgcagtg cttcaaatag cagcatgctt taacgttgat atatctgaat tgcttaccgg
      361 tgaagcacca agaaatagtg gttattcaat tactcgtaaa ggtgaaggtg taagtgtaga
      421 acgacattct caatataaat atcaggcttt ggcacatgat tttgcaaata aaaaaggaag
      481 tccatttgtg cttacaatag acgcaagcga tgaggaagtg cattataatc atcatattgg
      541 acaagaattt ttatatgtac ttgacggtac cattaaaata tattttaaag acgatgtgtt
      601 tagtctgaaa gaaggagata gtgtatattt tgatgcaaat attccacatg ccgtaaaaac
      661 agagaacggt gagcgagctc gtgttttagc agttgtgtct taattttata tatacaattt
      721 tactgttttt ttaataatac aattactcta gaaaaagtaa tgagtattat tttttgtatg
      781 aaaaaattct tttcgatact gtattttttt tgtgtattga gtaatatcta tgctcagtat
      841 actatacctc ataaaatgga atggtggtat catgaccgtt ttggtatgtt catacatttt
      901 ggttcatatt catactatgg taaaggagaa tgggtaatgc aaattgaaaa atggtcgaaa
      961 gatgcatatc aaactcaaat aacatcacaa ttcaatcctc ataattttaa tgcaaaagca
     1021 atagttgatg tagccaaatc taccggaatg aaatatatag taattacggc aaagcatcat
     1081 gaaggttttt gtatgtggaa aactaatgtt gcaggattta gagattatac tggaaataca
     1141 gtgtttgatt tatatcatta tataggtttt gaacgagata tacttaagga actcaaagat
     1201 gaatgtgatg ctcaaggcat taagttttgt ttgtattatt ctattttaga ttggaatcat
     1261 ccatcgcaaa cgttacacga atatttttcg gtaatgaaaa acatggaagc aaaaactgat
     1321 tatgtattgc aaatgaaagc tcaattacgt gaactaatta cactgtataa accagctgta
     1381 ttgtggtttg atggtgattg gtgtgaaaac gtgtatccac caacattaga aaattggtgg
     1441 aacaaagatg atgcaataaa tttatataat tacatactta gtatagattc tactataatt
     1501 ataaatgaac gggtaaaacg tgaattagga ttaggagatt ttatgtgtcc cgaacaaaaa
     1561 attcccgacg caccccttga acgacaatgg gaaacctgtc aaactatgaa tggtacttgg
     1621 ggatacgatg ctaaaagtta taatgcttat aattcggcac aatcatttat tcatgaattg
     1681 gaattgatag taagtcgtga tggtaattat ttacttaata ttgacccatt gcccgatgga
     1741 agtttaaacc ccaatatgct tgtgatatta catgaaatag gagaatggat gcgtgtgtat
     1801 ggtgaaagta tatatggctg taaacgaagt ccgtttttaa aacaacccga atggggtgta
     1861 tatacagcaa aagacaatat attgtatgct catatacaaa aaccaaatga agtaaagact
     1921 atagaaattc cactcaattc caagaaaatt aagtcagtat atgaagtgtc ttacaacaaa
     1981 caaaagttac aatatataaa aacatcacag ggaataatga ttgcagtacc taataaacaa
     2041 gtgtatccag caagtatagt tatagcagta gagtataaaa agtaatagat tattgtattc
     2101 cacgaatttt cttttcccaa ttccaagctg ttcgcataat ttctataaga ggtgtgtctg
     2161 ctttccaatt gaggagttta tttgcatatg aagtgcttgc ccatacttgt tctatatcac
     2221 cggcacgtct gggtgcaata gaataaggaa ctgtaactcc ggttgcttgt tcaaatgcat
     2281 gtatcatttc taaaactgaa acgccggttc cggtacctaa gttgcatatt tcacaagcct
     2341 tatttacgtt gttatttaga attctgttta aagaagcaac atgtgcttta gctaaatcta
     2401 ctacataaat ataatcgcga atagctgaac catcgggggt attataatcg tttccgaaaa
     2461 cttgcaattc ttttcgaata cctgcagcag tttgagttat aaatggaact aaattattag
     2521 gaattccttt gggtaattca ccaatacaag cgcttgggtg agcaccaatt ggattaaaat
     2581 atcgcaataa acttgcttta aacaaaggat tagatgttat ataatcttga attatatctt
     2641 cgcatatttg tttggtgttt ccatatggtg aagtagcttt ttgtaatgga gcttgttcgg
     2701 ttacgggtaa ttctgtgggt tgtccgtata cagtacatga tgatgaaaaa actaagttgt
     2761 tgcaattgtg agctcgcatt acgtctaata atactaataa tccatataca ttgttggtat
     2821 agtattccaa aggtttttgt accgattcgc caactgcttt gtaagcagca aaatgaataa
     2881 cagcatctat gggagagtgt tttgtaaaac aatttgataa ttcttgtttg ttacatatat
     2941 caatgtgttc aaatgccggt agtacaccgg taatatcata aataccttgt aatacacttt
     3001 gttctgaatt tgaaagatta tctacaacta ttactgaatg tttatgttga ataagttcaa
     3061 caactgtatg tgaacctata tatcctaatc cgccggttac cagaatatgt gccatatttt
     3121 attttttatt attccattta aatgaactaa ttagttctat gatatcttcg cgaataaatt
     3181 gaattgaagg agctaatgaa tctttatttg gtggtacatt aaagtataga gctcctctta
     3241 caaagtgttc aacactgtcg gttatgtaaa attgcataag agatgcggca tcacctttta
     3301 tatcaaatag taatccatgc acttgtttat cgttgtaatg aaattcttgt gtcttaatgg
     3361 catctgcttt tatatcatgt ttaaatacta agaaatgagc atcttctaca aatgttgata
     3421 aatcatgttt tacattttta tagcttatgt gaattgttgc ttttaatgtt ggaaattgaa
     3481 tattgagcca gtatggttca gcattgcgtg acgtatcaat ttgaatatat gaatatttag
     3541 gtatatcaaa tgaatatgga aatgagggga tagtagttga tatatactct cgttttggaa
     3601 aatctattct aaagtaacca cgttgtttag gcattgatat agttcgttct tcgcacgaaa
     3661 atagcaatat acatgatagt aaaactatac ttatacgcat cattttattc ttgtatgtta
     3721 agtttaattt tagaaattct ccgattatct tcttcttcaa ttaaaaactg taagtgttta
     3781 tagataattt gctgatgtaa ttttggaaaa tcgcctgtaa gttctagtat aaaaccggca
     3841 agagaatcgg catcaccttt atgtgaatca aaaaaatctt cgggtaattg tattatttta
     3901 caaacatcat gtatagatat ttttccttca aaaatatagg ttgtgtcatt tacttttgta
     3961 tacaactcat ctgattcgtc atcaaattca tcattaattt cacctaaaat ttcttctaat
     4021 atgtcttcga gtgtaacaat acccgatgtg ccaccatatt cgtctataac tacggctaga
     4081 tgaattttag ttttttgaaa ttcttcgagc agattgtcaa ttttttttgt ttcgggtata
     4141 aagtatgccg gacgaattaa tgtttgccat cggaaccctt gtgatttttc taaatgcggc
     4201 aaaagatctt ttacatacaa tattccttta atagtatcta atgtacccga aaaaacagga
     4261 acccgagagt atcctatatg tgtggtttcg tgtactaaat ctttaaaatt tgtagcaata
     4321 tccaaagcaa aaatacttac tctgggagtc ataatatttg atacaacttt ttgttgtaat
     4381 ttggcaatac cttctattat ttctttttct tcttttactt cgtgtgaact tacatgcaga
     4441 gcttcggcaa tatttatcat aacattttgc gaagtaagct gtgtaagtgt tt
//
LOCUS       DAOTOW010000087         4477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15869_length_4477_cov_3.493442, whole genome shotgun sequence.
ACCESSION   DAOTOW010000087 DAOTOW010000000
VERSION     DAOTOW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15869_length_4477_cov_3.493442"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2592
                     /locus_tag="P5243_02255"
     CDS             <1..2592
                     /locus_tag="P5243_02255"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS18300.1"
                     /translation="VQFQNTTQGTNIQSFRWEFGDGTQSTQNQVSHTYNGYGIYYAAL
                     RVMATNGCEGISMVAVRLEDFKPDIVISDGIKTLTSSNKACPGTVNFSMVNNGSSTLP
                     VTKYEWDFQNNGTIDSQNPTYSTTHAAGGFAQIKLTATTAACSKSVTKNYEIENVLQV
                     TYSPTDTFSCKLPFTVNYTAQSNIPNTTYQWFIDKNPYTGNPVQFTFLNEGFYSDSLI
                     ATSPNGCKTSLSKQKNVEIAVPKVSIVPISSPIEGCVPLTVDFNKTVTYNTISDFVKK
                     TEWDLDNNGIFEITADGPVSRTYTKRGVYPAVVKITTNRGCEYIDTTKEKYEIVRVGH
                     IGKGDLVFPDTILCASDILKFQYVNAIDSLIVTPFWDYISVIFEHVDDPLISSGTGTS
                     PMNYNLSAMFRDTVGEYSVKFQLNDHGCRKSYTDVDSSQCVVNYQLIGGDLVPIDTVC
                     PPTRIFVKGPILQLTSTPKDCNDNYNYSYYLTKMINVDTTKSGEYIEWYIQKFNGLGH
                     PISPKKLIAYNSDSIHINYDNDTLGRGNYMISVYAYNNNEDCVNGIKCNCIDSVFYTT
                     QVTDIKTVYTLQKSTVCVGDTAQFIKHPQSQDIESASWLYTRDSIFQHQIFPNIENDT
                     LFYIFNKRDISEVIVVGQDMYGCTDTARIPVKVYQPQANFFADIVSDCLPFQTQFTDS
                     TLHDTTIVARIWDFGNGFTQSGNDSITTTVYAQAGLTSPKLTVIDILGCSNVITRDKY
                     IKPVVPNSIFVVQHPKLCLNHEAAFIRNTSDPNYDNNIHKYSWDFGDGSTYDGTLLDT
                     TKHTYTQVTGITPYTVNLTAYSMSPEGNECMSSTTQTIDVKDFNPTIDTIGIDKCKNP
                     GKNFLFL"
     gene            2622..4160
                     /locus_tag="P5243_02260"
     CDS             2622..4160
                     /locus_tag="P5243_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS18301.1"
                     /translation="MAWYKEDGDVRKLISTNKYSFQVATFDNYGDQKLILYTTSNYYG
                     CELDSVIMDIEVPGYEAEFVADKYDVCVREDVTFTLTKQLNIDKYVSYWEFGDGNADY
                     ANIPQTTHSYTTLPGVLDNKFKVQFIVNAPNCKPRDIYKKVTIYPVLAEFDRGVQDAD
                     TIGCAPFTVTFYNKSAGLTASNFIWDFGDGTTSTEKNPTHTFNTTNSVYSVNLSITNN
                     VCPDNNTKPISTHPLANVVITADTTLCYGEQTTISASGDFSAITWSPASQITNVNSAT
                     TQYIPKKSGYIFANLQTNYGCTSSDSVFVYVQQYPRYQGAPDSLLLFYKTPTVLELAK
                     QPDSRIIAGQLYNVNNTPVQGVAYSWNPSTYLSCDNCISPNIDLQCGKPSYPSCIDFP
                     EYIDYTVHMVDSLGCFEADTTIRFHIIIETKAALPQAFTPNNDGENDFAFVRGWGIKE
                     FLEVKIYNRWGQLVFESNDLYKGWDGTYKGEPQAMDTYAYTIRIINTESKEEFIKGYI
                     TLLR"
     gene            complement(4244..>4477)
                     /locus_tag="P5243_02265"
     CDS             complement(4244..>4477)
                     /locus_tag="P5243_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18302.1"
                     /translation="MVGTAAPVMKKYIKHPTLWVAKHKIKSLLGKDLCLDDIPYYNVG
                     QNPLQGDLNYSRMTPFTDKAGVKPDPSLPLVDI"
BASE COUNT         1518 a          730 c          807 g         1422 t
ORIGIN      
        1 gtgcaatttc aaaatacgac acaaggcaca aatattcaat cgtttcggtg ggaatttggc
       61 gatggaactc agtcaacaca aaatcaggtt tcgcatacct ataatggcta cggcatatac
      121 tatgcagctc tgagagttat ggctacaaat ggctgcgaag gcatatcaat ggttgcggta
      181 cgattagaag attttaaacc cgatatagtt ataagcgatg gcattaaaac acttacctct
      241 tctaataaag catgcccggg aactgtgaat ttttcaatgg taaacaatgg ttcttctacc
      301 ttgcctgtta caaagtatga gtgggatttt caaaataatg ggactataga ttcacaaaat
      361 cctacatata gtacaacaca tgctgcaggt ggttttgctc aaattaaact gacagcaact
      421 actgctgctt gcagtaaatc ggttacaaaa aattatgaaa ttgagaatgt gctgcaagtt
      481 acatactcac ctaccgatac attttcatgt aaattgccat ttactgtaaa ttatacggca
      541 caaagtaata taccaaatac aacataccaa tggtttatcg ataaaaatcc ctatacaggc
      601 aatccggtac aatttacatt tttgaatgaa ggcttttatt ctgattcatt aattgctact
      661 tcacccaatg gatgtaaaac atcattgagc aaacaaaaaa atgtggaaat tgcagtacca
      721 aaagtttcaa ttgttcctat ttcttcgcct attgagggtt gtgtgccgct tacggttgat
      781 tttaacaaaa cggtaacata taatactata agtgattttg taaaaaaaac agagtgggat
      841 ttagataata atggcatatt tgaaattaca gcagatggtc cggtttcgcg tacatatacc
      901 aaacgaggag tgtatcctgc tgttgttaaa attactacga acagagggtg tgaatatatt
      961 gatacaacca aagaaaaata tgaaattgtt cgtgtagggc acataggtaa aggtgatttg
     1021 gtatttcccg ataccatatt gtgtgcttct gatattttaa aatttcaata tgtaaatgct
     1081 atagatagcc ttatagttac gccgttttgg gattatataa gtgtaatttt tgaacatgtt
     1141 gatgatcctt tgatttcttc gggaaccggg accagtccta tgaattataa tttatcggca
     1201 atgtttagag atacagtagg tgaatattcg gtaaagtttc aattaaatga tcatggttgc
     1261 agaaaaagtt ataccgatgt tgattcttcg caatgtgtag taaattatca attaataggg
     1321 ggcgatttag tgccaataga tactgtttgc cctcctacca gaatttttgt gaagggacca
     1381 attttacaac ttacatcaac tcctaaagat tgtaatgata attataatta ttcatactat
     1441 ttaactaaaa tgattaatgt ggatacaaca aaatcaggag aatatataga atggtatatt
     1501 caaaaattta atggtcttgg tcatccaata tcacctaaaa aattaattgc gtacaattct
     1561 gatagtattc atataaatta tgataatgat acattaggca ggggtaatta tatgatttcg
     1621 gtttatgcat acaacaataa tgaagattgt gtaaatggta ttaaatgtaa ttgtattgat
     1681 tcggtttttt atacgactca agttactgat attaaaacgg tatatacttt acaaaaaagc
     1741 acggtatgtg ttggtgatac tgctcaattt ataaaacacc cccaatctca agatattgag
     1801 tcagcaagtt ggttgtatac gcgtgatagt atttttcagc atcagatttt tccaaatatt
     1861 gaaaatgata ccttgtttta tatttttaat aagagagaca tatctgaagt tatagtagtg
     1921 gggcaagata tgtatggatg tactgatact gcccggattc cggtgaaagt atatcagcct
     1981 caagcaaact tctttgctga tatagtttcg gattgtttgc cgtttcaaac gcagtttacc
     2041 gattctacct tgcatgatac tactatagtt gctcgtatat gggattttgg taacggattt
     2101 acccaatcgg ggaatgattc tataacaaca acagtatatg ctcaagcagg attaacttca
     2161 cctaaactta ctgttataga tattttaggt tgtagtaatg ttataactcg cgataaatat
     2221 ataaaacctg tagtgcctaa ttctatattt gtagtacaac atcctaaatt gtgtttaaat
     2281 catgaagctg catttatacg aaataccagt gatccaaatt atgataataa tatccataaa
     2341 tattcttggg attttggtga tggcagtacc tatgacggaa cgttgcttga tactacaaaa
     2401 catacctata ctcaagttac cggtataaca ccatatacag taaatcttac ggcatatagc
     2461 atgagtccgg aaggaaatga atgtatgtcg agcacaacac aaacaattga tgttaaagat
     2521 tttaatccaa caatagatac cataggaatt gataaatgta agaacccggg caaaaatttc
     2581 ttgtttttat agataaaaca aaatattcaa aatattcaac agtggcatgg tacaaagaag
     2641 atggcgatgt tcgtaaactt atttctacca ataaatattc atttcaggtt gctacattcg
     2701 ataactatgg cgaccaaaaa cttatattgt atactacatc aaattattac gggtgtgagt
     2761 tggattcggt aattatggat atagaagttc ccggctatga ggcagaattt gttgccgaca
     2821 aatatgatgt atgtgttcgc gaagatgtaa catttacgct taccaagcag ttaaatattg
     2881 ataaatatgt atcatactgg gagtttggcg atggtaatgc agattatgca aatatacccc
     2941 aaacaacaca ttcttacact acgcttcccg gagttttgga taataaattc aaagtgcaat
     3001 ttattgtaaa tgcgcctaat tgtaaacctc gtgatattta taagaaagtc accatttatc
     3061 ctgtattggc agagtttgac agaggtgtgc aagatgccga tactataggg tgtgctccat
     3121 ttaccgtaac attttataat aaatctgcag gacttacagc ctccaatttt atatgggatt
     3181 ttggtgatgg cactacaagt accgaaaaaa atcctaccca tacatttaat acaaccaata
     3241 gtgtatattc cgtgaattta tctataacca ataatgtatg tcccgacaat aacacgaaac
     3301 ctatttcaac gcatccattg gcaaacgttg ttataacagc cgatacaaca ttatgctacg
     3361 gtgaacaaac aacaataagt gcttccggcg atttttctgc tattacatgg tcaccggcat
     3421 cgcaaattac caatgtaaat tcggctacaa cacaatatat tcctaaaaaa tcgggctata
     3481 tatttgcaaa tcttcaaaca aattatggct gtactagttc ggatagtgtt tttgtatatg
     3541 ttcagcaata tcctcgctac caaggagcac ccgattcgtt gttgcttttt tataaaacac
     3601 ctacagtgct tgaacttgcc aaacaacccg actcccgaat aattgcagga caattgtata
     3661 atgtaaataa tactccggtg caaggtgtgg catattcatg gaatccttct acatatttaa
     3721 gttgcgacaa ctgtataagt ccaaatattg atttacaatg tggtaaacca tcatacccaa
     3781 gttgtataga ttttcctgaa tatatagatt acacggtaca catggttgat tcgttaggtt
     3841 gttttgaagc agatacaacc attcgttttc atataataat tgaaaccaaa gcagcattgc
     3901 cacaggcatt tacaccaaat aacgatggcg aaaatgattt tgcatttgtt cgcggttggg
     3961 gtatcaaaga atttttggaa gttaaaatat acaatcgctg gggacaattg gtgtttgaaa
     4021 gtaatgattt atataaaggt tgggacggta cttacaaagg tgaaccccaa gcaatggata
     4081 cgtacgcata taccatacga ataattaata ccgaaagcaa agaagaattt ataaaaggat
     4141 atataacatt gttgcggtaa aaagtggtag tttgtataca aaatgaagaa tattatatga
     4201 aaaaacggta atcaattcat attgtatact ctagagtaaa atactatata tccaccaaag
     4261 gtaaactcgg gtctggtttt acccctgctt tatcagtaaa tggagtcatt cttgaataat
     4321 ttaaatcacc ttgcagtgga ttttgcccaa cgttgtaata tggaatatca tcaaggcata
     4381 agtctttgcc taacagtgat tttattttgt gttttgcaac ccaaagagta ggatgtttta
     4441 tatatttttt cataacagga gctgctgttc caaccat
//
LOCUS       DAOTOW010000088         4454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_15954_length_4454_cov_3.506706, whole genome shotgun sequence.
ACCESSION   DAOTOW010000088 DAOTOW010000000
VERSION     DAOTOW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15954_length_4454_cov_3.506706"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="P5243_02270"
     CDS             <1..596
                     /locus_tag="P5243_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRS18303.1"
                     /translation="GADAVKVGIGPGSICTTRVIAGVGVPQLSAIFAVAKALEKTDVP
                     VIADGGIRYSGDIVKALAAGASSIMAGSLFAGLEESPGETIIFQGRKFKTYRGMGSVE
                     AMQEGSKDRYFQDVEDDIKKLVPEGIAARVPYKGTLAETVYQLIGGLRAGMGYCGARN
                     IQELHKAKFVKITNAGHTESHPHDVTITREAPNYSRE"
     gene            630..2618
                     /locus_tag="P5243_02275"
     CDS             630..2618
                     /locus_tag="P5243_02275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS18304.1"
                     /translation="MNKHTIFLFLALQFASHFLCAQHIHIPDSIKNKILLTVDTAKIT
                     TDEFVWFYNKYNSYLDSSLKVGIDESMQLFIDYKMKIIDAERQKLDTTQKFKDEFYSY
                     IKSTAKPYLFNDTLEHLAKVQAYERLFTDYHISHIFVRANKFSNPKDTLTAYQKALKL
                     KKELLSGADFAKYAQLYSDDTFTKDKGGDMGYITSMLLPLPYENAVYAAKKGDLIGPV
                     CTDQGYYITKIHDIRPTKGQVKASLIIIYHDSKHADSIAKARLIADTVYNRLMAGESF
                     ETLSDIYNSNQKLASSKGDIGWLDNSTRYEPQLKEALFTLTNIGDITKPLNFDYGIVI
                     AKLTDKAELGDIHSYVKTINKNFKKDESRKNIVKDVFYAQYKKIAPYKEISNNVAEFI
                     TLVDNSILLSKWTKPTFTNNKVLFTIGSEQYRYTDFADYLVEYQKTKNIGEKEVLVRY
                     RLNEYVNKMLEQHAIQTLHTRNQEFARIMQEYHDGMIIYELLNNEVFQKANSDTIGVY
                     TWYTNHTNDYLQKEGVRVTYFICKNEKIIKKIIPLIEKQQVQKYNDQYIVKTINKKAM
                     DNVLLDSAEYYKGDNNFIDSLEWKKGSYHIISGNKILVIKDIIPIKPMTFESCKNAVI
                     SDYQNYLEEQWIQSLRKKYTYTLNDTYFNIILSKNPTE"
     gene            2615..3454
                     /locus_tag="P5243_02280"
     CDS             2615..3454
                     /locus_tag="P5243_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18305.1"
                     /translation="MKKLFKGFLILILIVSCNRNKNTDDQLLLAEVGEHTLYKKEVLE
                     SLPKNISSQDSIAFIKNYTDAWVRNKLLYEKAISNIHDKDGTIEGQVARFREQLYITA
                     YEQLFSNQKLDTIIPQNQIDEYYSNHKQEYILNVDVVKPIFAVIPKQKLTTKIKKYFY
                     SKDIDEFDIFKDFVYENSQKFYLGKDWVVLEQLKQEVPVELISNANIFDSKGIILEDS
                     QYAYCIKITEYVKAGNPKPKEMAYEEIATILLHKRKIELLKSMRNKLYQDALHKQYFQ
                     TYY"
     gene            3464..>4454
                     /locus_tag="P5243_02285"
     CDS             3464..>4454
                     /locus_tag="P5243_02285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS18306.1"
                     /translation="MKFLTILQSIVVICLSITTQAQSPIVIDQIIAVIGDEMIKQSDI
                     DNQAIQMKQFGAKDNDIKPCKIFEDMLFQKLLVHQSKLDSIQVSDSEIDAEINRRIEM
                     FIGEAGSLQKLETFYGKTELEIKTEWRPLIKEQILAQRVQSSLTGKIEVSPNEIRKYF
                     LGLNPDSIPTIPLQYEYAQIVIKPTLTDTEESEIKKKLEEIRQRAIKGENFSKLAVLY
                     SDDTESAKRGGLLGDYVSRGELVPEFAAAAFRLKEGEISRIVKTNYGYHIIQMIELKG
                     EKAKLKHILLRPKPSMATIQNTGKKADSVYALLRDTLKFERAALLYSSDEKTKY"
BASE COUNT         1784 a          639 c          684 g         1347 t
ORIGIN      
        1 caggtgctga cgctgtaaaa gtaggaatag gtcccggttc tatatgtact accagagtaa
       61 tagcaggagt tggtgttcct caattatcgg caatttttgc agttgcaaaa gcattagaaa
      121 aaactgatgt ccctgtaata gcagacggcg gtattcgtta ttcgggagat atagtaaaag
      181 ctttagccgc aggagcaagt tctataatgg caggttcttt atttgccgga ttagaagaat
      241 ctccgggtga aactattatt tttcaaggca gaaaattcaa aacatacaga ggtatgggtt
      301 ctgtagaagc tatgcaagaa ggatctaaag acagatattt tcaagatgtg gaagatgata
      361 ttaaaaaatt agttcccgaa ggtattgctg cccgagtacc atacaaagga actttggcag
      421 aaactgttta ccaacttata ggtggattgc gagcaggaat gggatattgc ggtgctcgta
      481 atatacaaga attacataaa gcaaaatttg taaaaattac aaatgcagga catacagaaa
      541 gtcatcccca tgatgttact attactcgtg aagctcctaa ttacagtaga gaataattgt
      601 tttatgctat tttaattatt gtatttaata tgaataaaca caccattttt ttatttcttg
      661 ctttacagtt tgcatcgcat tttttatgtg cacaacatat tcatatacct gattcaataa
      721 aaaataaaat attacttact gttgatactg caaaaataac aaccgatgaa tttgtttggt
      781 tttataataa atacaattca tatctggatt catcacttaa agttggaata gatgaatcaa
      841 tgcaattatt tattgattat aaaatgaaaa taattgatgc agaaagacaa aaacttgata
      901 caacacaaaa atttaaagat gagttttatt catatattaa atcaactgca aaaccttatt
      961 tatttaatga cacgttagag cacttagcaa aagttcaagc atatgaacga ttgtttaccg
     1021 attatcatat ttcacatatt tttgttcgag caaataaatt tagcaatcct aaagatactt
     1081 taacagcata tcaaaaagct cttaaactca aaaaagaatt attatcggga gctgattttg
     1141 ctaaatatgc tcaattatat tccgatgata catttacaaa agacaaaggt ggagatatgg
     1201 gatatattac gtctatgctt ctacctttac catatgaaaa tgccgtgtat gctgcaaaaa
     1261 aaggagattt aataggtccg gtatgtaccg atcaaggata ttatataacc aaaatacatg
     1321 atatcagacc tacaaaaggt caagtaaaag catctcttat tataatttat cacgactcaa
     1381 aacatgccga ttctatagca aaagcacgat taatcgctga tactgtatac aatagattaa
     1441 tggccggtga atcttttgaa acattaagtg atatatataa ttctaatcaa aaattagcat
     1501 cttcaaaagg tgatattggc tggctcgata attctacacg gtatgaacct caactaaaag
     1561 aagctttatt tactcttacc aatattggcg atattactaa acctttgaat tttgactacg
     1621 gtattgtaat agcaaaactt accgacaaag cagaattagg agatatacat tcatatgtaa
     1681 aaactattaa caaaaatttc aaaaaagatg aaagtagaaa aaatattgta aaagatgttt
     1741 tttatgctca atataaaaaa atagcacctt ataaggaaat aagtaataat gtagccgaat
     1801 ttattacact agttgataat tctattttac tttctaaatg gacaaaacct acatttacca
     1861 ataataaagt attatttact attggttcag aacaatatcg ctatacggat tttgccgatt
     1921 atttagttga atatcaaaaa acgaaaaaca ttggcgaaaa agaagtattg gttcgatata
     1981 gattgaatga atatgtaaat aaaatgcttg aacaacatgc tattcaaaca cttcataccc
     2041 gaaatcagga atttgctcgc ataatgcaag aataccatga tggtatgatt atatatgaat
     2101 tactaaacaa tgaagtgttc caaaaagcaa attctgatac tattggtgtt tatacatggt
     2161 ataccaatca tactaacgat tatttacaaa aagaaggagt tcgtgtaact tattttattt
     2221 gtaaaaatga aaaaattata aaaaaaataa ttcctcttat agaaaaacaa caggttcaaa
     2281 aatataatga ccaatatata gttaaaacta tcaataaaaa agcgatggac aatgttttgc
     2341 ttgattcagc agaatattat aaaggcgaca ataattttat tgatagttta gaatggaaaa
     2401 aaggttcata tcacataata tccggaaaca aaatacttgt aatcaaagat ataataccta
     2461 taaaacctat gacttttgaa tcgtgtaaaa acgctgtaat ttctgattat caaaattatt
     2521 tagaagaaca atggattcaa agtcttcgaa aaaaatatac atatactctt aatgatactt
     2581 attttaatat aattctttca aaaaacccaa ccgaatgaaa aaattattta aaggtttttt
     2641 aattctaata cttattgtat catgcaatag aaataaaaat accgatgacc aactattgct
     2701 tgccgaagta ggtgaacata ctctatataa aaaagaagta ttagaaagtt taccaaaaaa
     2761 tatttcatca caagatagta ttgcttttat aaaaaactat accgatgcat gggtgcgaaa
     2821 taaattatta tatgaaaaag ctatatcaaa tattcacgat aaagatggta ctattgaagg
     2881 acaagttgca agatttagag aacaattata tatcacggca tatgaacaat tattttcaaa
     2941 ccaaaaatta gatactataa tacctcaaaa ccaaatagac gaatattatt caaaccataa
     3001 acaagaatac atactgaatg tagatgtagt taaaccaatt tttgcagtaa ttcctaaaca
     3061 aaagctaact acgaaaatca aaaagtattt ttatagtaaa gacatagatg aatttgatat
     3121 atttaaagat tttgtttacg aaaactcaca gaaattttat ttaggcaaag actgggttgt
     3181 gcttgaacag ctcaaacaag aagttccggt agaacttatt tcaaatgcaa atatatttga
     3241 ctcaaaaggt atcattttag aagattctca atatgcatac tgtattaaaa ttacagaata
     3301 tgtgaaggcc gggaatccaa aaccaaaaga aatggcatat gaagaaattg ccactatttt
     3361 attacacaaa cgcaaaatag aattactcaa atcaatgaga aataaactat atcaagatgc
     3421 gttacacaaa caatattttc aaacgtatta ttaaagaaac ttcatgaaat ttttaaccat
     3481 tttacagagt attgttgtta tttgtttaag cattacaaca caagctcaat caccaatagt
     3541 tatagatcaa ataattgcag tgattggcga tgaaatgata aagcaatctg atattgataa
     3601 tcaggctatt caaatgaaac aatttggagc aaaagacaat gacataaaac cttgcaaaat
     3661 ttttgaagat atgctttttc aaaagctact tgttcaccaa tctaaattag atagtataca
     3721 agtatctgac agtgaaattg atgctgagat taatagaaga attgaaatgt ttattggcga
     3781 agcaggtagt ttacaaaaat tagaaacatt ttacggtaaa acagaactcg aaataaaaac
     3841 agaatggcgt ccacttatca aagaacaaat tttagcacaa cgtgttcaaa gttctttaac
     3901 cggtaaaatt gaagtttctc ctaatgaaat tagaaaatat tttttaggac ttaatccgga
     3961 tagtattcca accataccac tacaatacga atatgctcaa atagttataa aacctacact
     4021 aacagatacc gaagaatcag aaattaaaaa gaaactggaa gaaataagac aacgagctat
     4081 aaaaggtgaa aatttctcaa aattagcagt tttatatagc gacgatactg aaagtgctaa
     4141 aaggggagga ttacttggcg actatgtttc acgtggcgaa ttagttcctg aatttgctgc
     4201 tgcagctttt agacttaaag aaggtgaaat ttcaagaatt gtaaaaacca attatggata
     4261 tcatattatt caaatgatag agttgaaagg tgaaaaagca aaactaaaac atatcttact
     4321 acgcccaaaa ccaagcatgg caaccattca aaatacaggc aaaaaagccg atagtgtgta
     4381 tgcattgtta cgcgatactc tcaaatttga acgagctgct ttgctctatt catctgatga
     4441 aaaaactaaa tata
//
LOCUS       DAOTOW010000089         4424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16092_length_4424_cov_2.879835, whole genome shotgun sequence.
ACCESSION   DAOTOW010000089 DAOTOW010000000
VERSION     DAOTOW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16092_length_4424_cov_2.879835"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="P5243_02290"
     CDS             complement(<1..550)
                     /locus_tag="P5243_02290"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRS18307.1"
                     /translation="MRNKLIVVLFLFSTHFVYAHEGMWLPILIEQQILPEMKQAGCKL
                     TAEQIYSVNKASLKDAVVRIGSGCSGGFLSNQGLVITNHHCVQYFINQASNVANNYLE
                     KGFWAYSQGEELVIKGLSVSVLLEMRDVSQLILRKGDDTLSFTELQKEQDKRIKKIIE
                     TVQLEQPKIECSVEAFWGGNQYF"
     gene            complement(540..1112)
                     /locus_tag="P5243_02295"
     CDS             complement(540..1112)
                     /locus_tag="P5243_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRS18308.1"
                     /translation="MKQYHVILGSQSPRRKQLLQGLDIEFTVQVKDTEESYPSEMQLH
                     MVPEFIALNKFKAFIPQLTERDLVITADTIVLHNQTILHKPIDTDDAVRILQTLSGSS
                     HTVITGVCYGTKQKYLTFSAQSQVHFAHLTQADIDYYVTTYMPMDKAGAYGVQEWIGY
                     IGIQGIEGSYFNVMGLPVQMLYQKLLEYEE"
     gene            complement(1140..2096)
                     /locus_tag="P5243_02300"
     CDS             complement(1140..2096)
                     /locus_tag="P5243_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HRS18309.1"
                     /translation="MIHILQLVRYKNLSIIIATMLVVRYGLILPMLQQDYEILNVELD
                     LQFSLFHFILLMIATVSLSASGYIINDYFDVNADMMNKPDKVIIGKYIHRRFAMFLHW
                     FLNIVGVICGGIASYAIGFPHFVMIFVFIAGLLWFYSTTFSREPFIGNILIAVLVALV
                     PLITAVYELLPLNFMYKHILNTMYVSFEGMLFWSLGYALFAFLLTLIREMVKDIEDLE
                     GDAAYGRNTIPIAFGIEWARYITISVFVVTIGALLFVLFAYLQQGFAQLYMIIGIIIP
                     LILTLILFIVSNSAQTYHRVSTLLKIIMILGLLYIISKNILF"
     gene            complement(2208..2723)
                     /locus_tag="P5243_02305"
     CDS             complement(2208..2723)
                     /locus_tag="P5243_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRS18310.1"
                     /translation="MFKDKLKHVKAFIFDVDGVFTNGYIYISADGKQTRCMNVKDGYA
                     VHFAIKKGYHIGIISGGKCESIVHRFKDLGVYDVYLSSHDKMVDYDDFCAKYNLQEKD
                     IFYIGDDLPDYNVMKRSGVCACPADAAHEIQLISNYISSKPGGQGCVREIIEQTLRAQ
                     NKWMDIDAFVW"
     gene            complement(2731..3510)
                     /locus_tag="P5243_02310"
     CDS             complement(2731..3510)
                     /locus_tag="P5243_02310"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRS18311.1"
                     /translation="MQIKAVIIGAGNVGTHLAIALKNEGVQILQIVSRTMKSAVELAS
                     RMECGFTTDIHEINKNADIYIVCVTDNAIIPVLRQLQIGNKFIVHTSGSMGIEVFYDL
                     AQNYGVVYPMQTFSKFKDINYAEIPIFIEANTPENEQMLFNFVKKISPHVQVINSQQR
                     CMLHICAVFANNFTNHMCTIAELLMKENQIKFETFKPLLRETFEKITKYSPSISQTGP
                     AMRKDIHVVQKHIDILAQYPSFQEIYRNISNSILEMYKKEE"
     gene            complement(3606..3893)
                     /locus_tag="P5243_02315"
     CDS             complement(3606..3893)
                     /locus_tag="P5243_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18312.1"
                     /translation="MKKIVLFSIILISVVSCSNNNVKKDAERMAELFCKQNKIVSQAQ
                     DLHDSTVLKQLQQIEEEANKINKELETKYANDTKSMELFAKTYQERILKCE"
     gene            complement(3905..>4424)
                     /locus_tag="P5243_02320"
     CDS             complement(3905..>4424)
                     /locus_tag="P5243_02320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRS18313.1"
                     /translation="KVLLFSASNSSQSINQTFIEHIAQYIHQHEVTIIDLRFFPAPLY
                     SYDIEQKSGKPSAMMALHNLFSSHDAFIIATPEHNGALPAFFKNIVDWLSRIEKKIFQ
                     QKPVILFSVTPGAGGSKLVLQHATDLIPRWGGNLQATIGIPNYIDFENFETYFQANQT
                     LLSHVQKTLQLL"
BASE COUNT         1514 a          778 c          568 g         1564 t
ORIGIN      
        1 aaaaatattg atttccaccc caaaatgctt caacagaaca ttcaattttt ggttgttcca
       61 attgtactgt ttctattatt ttttttattc gcttatcttg ttctttttgt aattcagtaa
      121 atgacaacgt atcatcacct ttacgcagta ttaattgact aacgtctcgc atttcaagta
      181 aaacagaaac acttaacccc tttattacaa gctcttcacc ttgcgaataa gcccaaaaac
      241 ctttttccaa ataattattc gcaacattac ttgcttgatt aataaaatat tgcacacaat
      301 gatgattcgt aattactaac ccctgatttg ataaaaatcc tccactgcaa ccactaccaa
      361 ttcttaccac agcatctttt aacgatgctt tattcacact atatatttgt tctgcagtaa
      421 gcttacaccc tgcttgtttc atttcgggta atatttgttg ttctataagt ataggcaacc
      481 acataccttc gtgggcatat acaaagtgtg ttgaaaacag aaacaaaaca actattagtt
      541 tattcctcat attccaacaa tttctgatac aacatttgca caggtaatcc cattacatta
      601 aaataagacc cttctatacc ttgaatacct atatacccta tccattcctg aacgccatat
      661 gctcctgctt tatccattgg catataagta gtaacataat agtctatatc tgcttgtgta
      721 agatgagcaa aatgcacttg actttgagca gaaaatgtca aatatttttg ttttgtacca
      781 taacatacac cggtaatcac tgtatgacta cttcctgaca atgtttgtaa tatccttaca
      841 gcatcatctg tatctattgg tttgtgtagt attgtttgat tatgcaatac aatagtatcg
      901 gcagttatta ccaaatctcg ttctgttaat tgtggaataa atgctttaaa tttattaagt
      961 gctataaatt cgggcaccat atgtaattgc atttcagaag gatacgattc ttcggtatct
     1021 ttgacttgaa cagtaaattc tatatctaat ccttgcaaaa gttgtttacg acgaggtgat
     1081 tgagaaccaa gaatgacgtg atattgtttc aacttttctt gtaatctgtt cattacaaat
     1141 taaaataaaa tatttttact aataatatac aataatccaa gtatcataat tattttaagc
     1201 aatgtactta ctctgtggta tgtttgtgcc gaatttgata ctataaacaa tataagtgtt
     1261 agtattaatg gtattataat tccaataatc atatacaatt gtgcaaatcc ctgttgaaga
     1321 tatgcaaaca aaacaaacaa caatgcacca atagtaacaa caaatacact tattgttata
     1381 tatcgagccc attcaatacc aaaagctatt ggaattgtgt ttctaccata cgcagcatcg
     1441 ccttccaaat cttcaatatc ttttaccatt tcacgaataa gtgtaagcaa aaaagcgaat
     1501 agtgcatatc ccaaactcca aaacaacata ccttcaaaac tcacatacat ggtattaaga
     1561 atatgtttgt acataaaatt gagaggtaat agttcatata cagccgtaat caatggaact
     1621 aatgctacta atacagcaat aagtatatta ccaataaaag gttctctgct aaaagttgta
     1681 gaataaaacc ataataaccc cgctataaaa acaaaaatca ttacaaaatg aggaaatcca
     1741 atagcatacg atgctattcc gccacagatt acaccaacaa tattaagaaa ccaatgtaaa
     1801 aacatagcaa atcgcctatg aatgtattta ccaataatta ccttatctgg tttattcatc
     1861 atatcggcat ttacatcaaa ataatcattt ataatatatc ccgatgctga caatgaaaca
     1921 gttgctatca ttaataaaat gaaatgaaac aatgaaaatt gcaaatcaag ttcaacattt
     1981 aatatttcat aatcttgttg gagcattggc aatataagac catatcgtac aaccaacatg
     2041 gttgcaatta taatagaaag atttttgtaa cgaacaagtt gtaatatatg aatcattagt
     2101 ttcatgagtg gtataaaata cttctatatt attgacacaa tatacaaaaa attaaaaata
     2161 tacatgtcat tacattgttt atttgttcaa ttacaaataa tatactttta ccaaacaaac
     2221 gcatcaatat ccatccattt attttgagct cgaagcgttt gttctattat ttcacgtaca
     2281 caaccttgac ctccgggttt tgatgaaata taattactta taagttgaat ttcgtgagca
     2341 gcatcagcag ggcaagcaca tacgccagaa cgcttcataa cattataatc cggcaaatca
     2401 tctcctatat aaaaaatatc tttttcttgt aaattatact tagcacaaaa atcatcataa
     2461 tctaccattt tatcatgcga agatagatac acatcataaa cacctaaatc tttgaatctg
     2521 tgaactatag actcacattt acccccactt ataataccta tatgatagcc tttttttata
     2581 gcaaaatgaa ctgcataacc gtcttttaca ttcatacacc gtgtctgttt gccatctgca
     2641 gaaatgtata tataaccatt tgtaaataca ccatcaacat caaaaataaa agcctttacg
     2701 tgttttaatt tatctttaaa catacccatg ctattcttcc tttttataca tttctaatat
     2761 actattactt atatttctat aaatttcctg aaaacttgga tactgagcaa gaatatcaat
     2821 atgtttttgc acaacatgaa tatcctttct cattgccggt ccggtttgcg aaattgatgg
     2881 tgaatatttt gttatttttt caaacgtttc tctaagcaaa ggtttaaacg tttcaaattt
     2941 aatttgattt tctttcatca acagttctgc aattgtacac atatgattag taaaattatt
     3001 ggcaaatact gcacatatat gcaacataca tcgttgttga gaatttataa cttgtacatg
     3061 aggactgatt ttttttacaa aattaaatag catttgttcg ttttcgggag tatttgcctc
     3121 aataaatata ggaatttcag cataatttat atccttaaat ttcgaaaatg tttgcatagg
     3181 atatacaaca ccataatttt gtgccaaatc ataaaatact tctatcccca tacttcctga
     3241 ggtatgtaca ataaatttat taccgatttg taattgtcgt aatacaggta ttatggcatt
     3301 atcggtaaca caaactatgt aaatatcagc gtttttattt atttcatgaa tatctgttgt
     3361 aaaaccacat tccatacgtg aagctaattc aacagcagat ttcattgttc tgcttacaat
     3421 ttgcaaaatt tgtacacctt catttttaag tgcaatagcc aaatgcgtcc caacattacc
     3481 tgctcctatt ataacagctt ttatttgcat gaaaaattgt aatttaatgt tcactacaaa
     3541 agtagtaaaa tacttgagta aattacaaag ttttatatta attgttggta ttgaattgca
     3601 tcaaattatt cgcatttcaa aatacgctct tggtaggttt ttgcaaacaa ttccatagat
     3661 ttagtatcat ttgcatactt agtttccaat tctttattaa ttttatttgc ttcttcttct
     3721 atttgttgta actgctttag aactgttgaa tcatgtaaat cttgtgcttg cgatactatt
     3781 ttattttgtt tgcaaaataa ttctgccatt cgttccgcat ctttctttac attgttgtta
     3841 ctacatgaaa caactgatat tagaattatt gaaaatagta ctattttttt cataatcgta
     3901 taatttataa taattgtaat gttttttgca catgagataa tagcgtttga ttggcttgaa
     3961 aatatgtttc aaaattttca aaatctatat aatttggtat tccgatagtc gcttgtaagt
     4021 ttccacccca tcgaggaatt aaatctgttg catgttgcaa tactaatttt gaaccaccgg
     4081 cacccggtgt taccgaaaat aaaataaccg gtttttgctg aaatattttt ttctctattc
     4141 ttgacaacca atcaactata tttttaaaaa aagccggtag tgcgccatta tgttccggtg
     4201 tggcaattat aaaggcatcg tgggaagaaa ataaattatg caatgccatc attgccgaag
     4261 gttttcccga cttttgttca atatcataac tatatagagg tgcaggaaaa aatcgcaaat
     4321 ctattattgt tacttcatgt tgatgtatat attgagctat gtgttcaata aacgtttgat
     4381 taatagattg agaactatta ctcgctgaaa ataataatac tttc
//
LOCUS       DAOTOW010000090         4385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16260_length_4385_cov_2.700231, whole genome shotgun sequence.
ACCESSION   DAOTOW010000090 DAOTOW010000000
VERSION     DAOTOW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16260_length_4385_cov_2.700231"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /gene="rplP"
                     /locus_tag="P5243_02325"
     CDS             complement(<1..211)
                     /gene="rplP"
                     /locus_tag="P5243_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRS18314.1"
                     /translation="MLQPKKTKFRKQQKGRMKGNAQRGHELAFGSFGIKSLETCWITS
                     RQIEAARIAVTRYMKREGQIWIRIFP"
     gene            complement(285..926)
                     /gene="rpsC"
                     /locus_tag="P5243_02330"
                     /pseudo
     CDS             complement(285..926)
                     /gene="rpsC"
                     /locus_tag="P5243_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(937..1347)
                     /gene="rplV"
                     /locus_tag="P5243_02335"
     CDS             complement(937..1347)
                     /gene="rplV"
                     /locus_tag="P5243_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRS18315.1"
                     /translation="MSNRKKIKADQIKDAKKLKAFAILRNCPSSPRKMRLVADMVRGK
                     EVNNALDLLRYSTKESSRKVEKLVLSAINNWQQKSEGKRIEDNQLYIKEIFVDEGRML
                     KRIQPAPQGRAHRVRKRSNHVTVVVDTIQLETEA"
     gene            complement(1358..1633)
                     /gene="rpsS"
                     /locus_tag="P5243_02340"
     CDS             complement(1358..1633)
                     /gene="rpsS"
                     /locus_tag="P5243_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRS18316.1"
                     /translation="MSRSLKKGPYIDFNLERKVLQMNESGKKTVIKTWSRASMISPDF
                     VGHTIAVHNGNKFIPVYVTENMVGHKLGEFSPTRNFRGHAGKKNKKK"
     gene            complement(1652..2476)
                     /gene="rplB"
                     /locus_tag="P5243_02345"
     CDS             complement(1652..2476)
                     /gene="rplB"
                     /locus_tag="P5243_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRS18317.1"
                     /translation="MAVKKLKPVTPGQRHKIVAVNTGLSKVKPEKSLVETLKRTGGRN
                     NTGKMTMRYIGGGHKKKYRIIDFKRDKENIQAEVLTVEYDPNRSANICLVQYTDGEKR
                     YIIAPQGIKVGQTIASGEGVEPEIGNSLYLKEIPLGTVIHNIELTPGKGAQLARSAGT
                     YAQLASRDGKYAIIKLPSGETRMILTSCKATIGAVSNSEHSLIRSGKAGRSRWLGRRP
                     RVRGVVMNPVDHPMGGGEGRASGGHPRSRKGLPAKGYKTRAPKKASSSLIIERRKK"
     gene            complement(2481..2771)
                     /gene="rplW"
                     /locus_tag="P5243_02350"
     CDS             complement(2481..2771)
                     /gene="rplW"
                     /locus_tag="P5243_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRS18318.1"
                     /translation="MEILQRPLITEKMSKKADTLNQYGFIVDKNADKTQIKKAVEEMY
                     GVTVESVNTIRYAGKVKTRNTKSGVVVGKTNSYKKAIVSLKQGEQIDFYSNI"
     gene            complement(2778..3407)
                     /gene="rplD"
                     /locus_tag="P5243_02355"
     CDS             complement(2778..3407)
                     /gene="rplD"
                     /locus_tag="P5243_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRS18319.1"
                     /translation="MELVVKDIQGKDTKKKVVLDESIFCIEPNEHAVYLDVKAILANK
                     RQGTHSSKERNAVAGSTRKIKRQKGTGGARAGSLNNPLFRGGGRIFGPEPRDYSQKVN
                     KKLKDLARKSVLSSKVKDNAITVVEDFDFEVPKTKKLLEIKENLKIDNKNSLLILPNI
                     NKNVYLSSRNLQGFLVVSASEINTYDLLKAKNVILFESSVEEIEKNLKN"
     gene            complement(3407..4027)
                     /gene="rplC"
                     /locus_tag="P5243_02360"
     CDS             complement(3407..4027)
                     /gene="rplC"
                     /locus_tag="P5243_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRS18320.1"
                     /translation="MLGILGKKLGMTSIFLPDGKNIACTVIETGPCVVTQIKTKETDG
                     YNSVQLAFDEKKEKNTTRAELGHFKKANTSPKRSVVEIRDFDKEVNLGDVLDVSSIIE
                     VGDWLDIIGTSKGKGFQGVVKRHNFGGVGESTHGQHNRLRAPGSIGACSTPSRVFKGM
                     RMGGRMGGNQVKVENLEVVKVLPEKNVVLVKGAVPGAKGGYLIMTK"
     gene            complement(4049..4354)
                     /gene="rpsJ"
                     /locus_tag="P5243_02365"
     CDS             complement(4049..4354)
                     /gene="rpsJ"
                     /locus_tag="P5243_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRS18321.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKTTGAVVCGPIPLPTH
                     KRIFTVLRSTHVNKKAREQFQLASHKRLLDIYSSTSKTVDALMKLELPSGVEVEIKV"
BASE COUNT         1226 a          847 c          564 g         1748 t
ORIGIN      
        1 caggaaaaat tctaatccaa atttgacctt cacgtttcat ataacgtgta actgcaatac
       61 gagcagcttc aatttgacgc gatgttatcc aacaagtttc aagagatttt ataccaaatg
      121 atccgaatgc tagttcgtga cctctttgag cattaccttt cattctccct ttttgttgct
      181 ttctgaattt tgttttttta ggttgtaaca ttgctgtata ttcttaatta ttattttttt
      241 ctttttctca tagatttttt aggtgcagca gtcaaattgc tattaggaga taaatcaact
      301 tttccataaa tctcaccttt aaaaatccat acttttaatc ctaatttacc ataagttgta
      361 gaagcagctg cagttgcata atcaatatca gcgcgtagtg tatgtaatgg tgttcttcct
      421 tctttataca tttctgaacg tgccatttct gctccattta atctaccaga aatttgcact
      481 ttaatacctt cggctcccat tctaattgca gaagctattg ccatttttat agctctacga
      541 tatgcaacac gaccttctaa ttgacgagct atactgtctg caacaattat agcatctaat
      601 tcaggacgtt ttacttcaaa tatattgatt tgaacttctt tattagaaat ctttttaagt
      661 tcttctttta gtttatcaac ttcttgacct cctttaccta taataattcc tggacgagca
      721 gtatttatag taatggttac taatttaagt gttctttcaa ttataatttt tgagatacta
      781 gcttttgcaa gacgagcata taaatatcgt cttattttgt aatcttcatg tagtttttca
      841 acataatcat taccaccata ccagttagaa tcccaacctt tgataattcc taatctattt
      901 cctattggat taactttttg tcccatgaat ataattttat gcttctgttt ctaattgaat
      961 agtatctacc actactgtta catgatttga acgttttcga actctatgag ctcttccttg
     1021 aggtgccggt tgaatacgtt ttaacattct accttcatca acgaatattt ctttaatata
     1081 taattgatta tcttcaattc gttttccttc acttttttgt tgccaattgt taatagcact
     1141 caaaactaat ttttcaactt ttcttgaaga ctcttttgta gaataacgca ataaatcaag
     1201 tgcattattt acctcttttc ctctaaccat atcagcaacc aatcgcattt tacgtggtga
     1261 cgatgggcaa tttcttaaga tagcaaaagc tttaagcttt ttagcatcct taatttggtc
     1321 agcttttatt ttctttctat tactcatgac ttagttctta tttcttttta ttctttttac
     1381 ctgcatgtcc tctaaaattt cttgttggtg aaaattctcc aagtttatgt ccaaccatat
     1441 tttcggttac atacaccgga ataaatttat taccattatg aacagcaatt gtatgcccaa
     1501 caaaatcggg agaaatcata gaagctcttg accaagtttt gattactgtt tttttcccac
     1561 tttcattcat ttgtaaaact ttacgttcca aattgaaatc tatatatgga ccttttttaa
     1621 gtgaacgact catattacgt tttcaattaa attatttttt tcttctttca attattaaac
     1681 tgctcgatgc tttttttggt gcacgtgttt tatatccttt agcaggtaac cctttacgtg
     1741 aacgaggatg tcctcctgaa gcacgtcctt caccaccacc cattgggtga tcaacaggat
     1801 tcataacaac accacgtact cgtgggcgac gacctaacca tctacttcta ccagcttttc
     1861 ctgaacgtat taatgaatgt tcagaattag aaacagcacc tatggtagct ttgcatgatg
     1921 ttaatatcat acgggtttca ccagaaggca atttaataat tgcatattta ccatctcttg
     1981 atgctaattg agcataggtt cctgcacttc tagcaagttg tgctccttta cctggagtta
     2041 attctatatt atgaatcact gttccaagcg gaatttcttt taaatataaa ctgtttccga
     2101 tttctggttc aacaccttct ccagaagcaa ttgtttgacc aactttaatt ccttggggtg
     2161 caattatata tcttttttca ccgtcagtat attgaactaa acaaatattt gcacttctat
     2221 ttggatcata ttcaacagtt aaaacttctg cttgaatatt ttctttatca cgtttaaaat
     2281 caataatacg atattttttc ttatgaccac caccaatata acgcatagtc atttttccgg
     2341 tattatttct accacctgtt ctttttaatg tttcaacaag tgatttttca ggttttactt
     2401 ttgataatcc tgtattaacc gctacaattt tatgtctttg tccgggagtt accggtttca
     2461 acttttttac tgccattgtt ttatatatta ctatagaagt ctatttgttc tccttgtttt
     2521 aaagatacta ttgctttttt atatgaatta gtttttccaa ctactacacc acttttagta
     2581 tttctagttt ttactttacc ggcataacga attgtattta ctgattctac ggtaacacca
     2641 tacatttctt ctaccgcttt ttttatctga gttttgtcag catttttatc tacaataaac
     2701 ccgtattggt ttaaagtatc agcttttttt gacatttttt cagttattag tggtctttgt
     2761 aaaatttcca ttaccaatta attttttaaa tttttttcaa tttcttctac cgaactctca
     2821 aaaagtatta catttttcgc ttttaacagg tcgtaagtgt ttatttcaga agcacttaca
     2881 actaaaaacc cttgtaaatt tcgcgatgac aaatatacat ttttatttat atttggtaat
     2941 attaataaag agtttttatt atcaatttta agattttctt tgatttcaag taactttttg
     3001 gttttaggta cttcaaaatc aaagtcttct actacagtaa ttgcattatc tttaactttt
     3061 gaagacaata cagatttacg agctaaatct tttaattttt tatttacttt ttgcgaataa
     3121 tcacgtggtt caggaccaaa tattctacct cctccacgga ataatgggtt atttaaacta
     3181 cccgcacgcg ctcctcctgt acctttttgt cttttaattt ttcgagtact accagctacg
     3241 gcatttcttt cttttgagct atgagttcct tgacgtttat ttgcaagtat agctttaaca
     3301 tctaaataaa ctgcatgttc atttggttcg atacagaaaa ttgattcatc taaaaccact
     3361 ttctttttgg tatcttttcc ttgtatatct ttaactacta attccattat ttagtcatta
     3421 ttaaatatcc acctttagct ccgggaacag ctcctttcac caatactaca tttttttcag
     3481 gtaatacttt taccacttct agattctcaa cttttacttg attgcctccc attcttccac
     3541 ccattctcat gcctttaaat acgcgagaag gagtagaaca tgcaccaata gaaccaggtg
     3601 cacgtagacg attatgttga ccatgtgtac tttctccaac accaccaaaa ttatgtcgtt
     3661 taacaacacc ttggaatcct tttccttttg atgttccgat tatatctaac cagtcgccaa
     3721 cttcaataat gcttgataca tcaagtacat cacctaaatt aacttcttta tcaaaatcgc
     3781 gaatttctac aacagatcgt tttggagaag tatttgcttt tttaaaatga cctaattctg
     3841 cacgtgtagt atttttctct tttttctcat caaaagctaa ttgaactgag ttatacccat
     3901 ctgtttcttt agtttttatt tgagtaacta cacaaggacc tgtttctata acagtacatg
     3961 caatattttt accatcgggt aaaaatatag aagtcatacc taattttttt cctagtattc
     4021 cgagcatttt tacaaattta ttacgttatt acactttaat ttcaacttct acaccacttg
     4081 gtaactctaa tttcataaga gcatcaactg tttttgaagt actactgtaa atatccaata
     4141 gtcttttatg cgatgccaac tgaaattgtt cacgagcttt tttatttacg tgagtagaac
     4201 gtaatactgt aaaaattctc ttgtgagttg gcagcggaat cggaccacat actacagctc
     4261 ctgtagtttt aaccgtttta acaatctttt cggcagactt gtctacaaga ttgtggtcat
     4321 atgatttcaa cttaattctg attttttgac tcatgccttt aattatatta attgtattta
     4381 tttac
//
LOCUS       DAOTOW010000091         4301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16656_length_4301_cov_2.905087, whole genome shotgun sequence.
ACCESSION   DAOTOW010000091 DAOTOW010000000
VERSION     DAOTOW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16656_length_4301_cov_2.905087"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="P5243_02370"
     CDS             complement(<1..1479)
                     /locus_tag="P5243_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18322.1"
                     /translation="MVYCIAMQVSAYAQFTITKYTYWFNNDVSQAVTVATPPQEILEI
                     DERISTNHLPKGLHTFNIQFFDANELASSVVSHFFTNLPLLSASTQTIVACEYWFDSN
                     YAQAITKTVTAASVVTVLDSVSISQLTDGLHTCNIRFKNSDGIYSSVVSSFFTKLPQQ
                     VLQLNTITSFELWFDSDYTNKQVMSVTPGASIIMLDSIVASQLTSGLHTCNIRFKDSQ
                     NRYSSVVSQFFTKFSVPITVSNSVDICEYWFDADYGNKQTKNISNSYIIEFLDSIQTA
                     SLTNGLHTFNIRFRNSQGVWSSPVSSFFTKYSESLVTVRTITEYEYWFDTDISQVVKK
                     ICSTPITTLSLIDSIQTDNITTGLHTLTFRFKDNTGKWSPSISSYFTKLPYFPATEST
                     IVGYQYWFDADITTKKYTQVLAQTRVEILEDIDVSSLPNGFHTIIFQFKYANNTWSSP
                     ITHYFVKEQSTIIQPVISRLAYWFDDDEHTNTIILLQPTQVTT"
     gene            complement(1521..2561)
                     /locus_tag="P5243_02375"
     CDS             complement(1521..2561)
                     /locus_tag="P5243_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRS18323.1"
                     /translation="MKKILCYAIPFALVCFGFSTGYAQTRVMLHHAGTVTAYSGINAF
                     VAANTAAVNGDTIYLSGGFFTSATISKKLVVIGTGVFPDSTSATGISRLTSTLSLAAG
                     SDSTIIEGLYIDGNINFAANTAIKDVLIRRNEFNELNITGSSRNNLRTIINQNIIKGP
                     VSVENADALIFANNIFHHHMYNVSNALIEHNNIITSAGSWSVLLNGISDCLIKNNIFK
                     ISFGYAASFLSGNNNVFQNNILNFALSGGTQTYSNNLQVHSDSIFVNDITPYTINYGS
                     NYHIRPYLYTTASTYATDGTEVGIYGGSEQFKFKEALVPRNPHIISKEIDQTTSADGK
                     LRVKIKVSAQNK"
     gene            complement(2724..3926)
                     /gene="trpB"
                     /locus_tag="P5243_02380"
     CDS             complement(2724..3926)
                     /gene="trpB"
                     /locus_tag="P5243_02380"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRS18324.1"
                     /translation="MSHNYFKTYPSKEGFFNEYGGAFIPPVLESEMKKITDAYFTISK
                     SHDFINELRSIRKHYQGRPTPVYYCNRLSQKYGGRIYLKREDLNHTGAHKLNHCMGEA
                     LLAKYLGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEHPNVVRMKILG
                     ATVVPVTHGLKTLKEAVDSAFEAYLKDPVNSIYCIGSVVGPHPFPMMVRDFQRVVGIE
                     AREQYLEMTGELPDSVVACVGGGSNAMGLFSAFLEDEEVSIYGVEPGGRSMNLGDHAA
                     TMTFGTPGIIHGFKCYTLQDEKGEPSPVYSVASGLDYPGVGPEHSMLKDIKRVTYDVI
                     NDKECIEAFYELSRLEGIIPALESSHAVAYALKLAQQYPQSSILVNLSGRGDKDIDFV
                     VDTFGLPK"
     gene            complement(4006..4227)
                     /gene="yidD"
                     /locus_tag="P5243_02385"
     CDS             complement(4006..4227)
                     /gene="yidD"
                     /locus_tag="P5243_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRS18325.1"
                     /translation="MCTVCNYILIIPIKLYQWFISPWLPNSCRYTPTCSQYGIEAIQK
                     YGVFKGGYMLIKRLLRCHPWGGHGHDPVV"
BASE COUNT         1459 a          776 c          647 g         1419 t
ORIGIN      
        1 tgtagttact tgtgttggct gcaacaatat tattgtattt gtatgttcat catcatcaaa
       61 ccaatacgca aggcgtgaaa taaccggttg aattatagta gattgttctt ttacaaaata
      121 atgagttata ggtgaactcc aagtattatt ggcatactta aattgaaata taatagtatg
      181 aaacccattt ggcaatgaag aaacatcaat atcttccaag atttctacac gtgtttgtgc
      241 aagaacttgt gtatattttt ttgttgttat atctgcatca aaccaatatt gatacccgac
      301 tattgtactt tctgtagccg gaaaatatgg caattttgta aaatatgaac ttattgaagg
      361 actccacttg cctgtattat ctttgaaacg aaaagtaagt gtatgcaatc ccgtagtaat
      421 attgtctgtt tgaatagagt ctataagcga gagtgtagta ataggggtgc tacatatttt
      481 tttcacaacc tgcgatatat ctgtgtcaaa ccaatattca tattcggtaa ttgtgcgaac
      541 agttacaagc gactcggaat attttgtaaa aaaagaactt accggagacg accaaactcc
      601 ctgactatta cggaatctaa tattaaatgt atgtaatccg tttgtaagag aagctgtttg
      661 tatagaatct aagaattcta ttatataact attgcttata ttttttgttt gtttattccc
      721 gtaatcagca tcaaaccaat actcacaaat atcaaccgaa tttgatacag taattggaac
      781 agaaaatttt gtaaaaaatt gactaactac tgaactgtat ctattttgtg aatctttgaa
      841 acgaatatta catgtatgta atcctgatgt taattgcgat gcaactatag aatcaagcat
      901 tattatactt gctccgggag taaccgacat cacttgttta ttagtataat cagaatcgaa
      961 ccaaagctcg aacgatgtaa ttgtatttaa ttgtaaaact tgttgtggaa gttttgtaaa
     1021 aaaactactt actacactac tatatatacc atctgaattt ttgaaacgaa tattacacgt
     1081 atgtaaaccg tcagttaatt gacttatcga tacggaatcc aaaacagtaa ctacagatgc
     1141 tgccgttact gtttttgtaa ttgcttgagc ataattagaa tcaaaccaat attcacaagc
     1201 tactattgtt tgtgttgatg ccgacaatag aggtaaattt gtaaaaaaat gactaacaac
     1261 tgaacttgcc aattcatttg catcaaaaaa ctgtatatta aacgtatgta accctttagg
     1321 taaatgattg gtagatatac gttcgtctat ttccaatatt tcttgtggtg gtgtagctac
     1381 tgtaacagct tgtgacacgt cattattgaa ccaataggtg tatttagtaa tagtaaattg
     1441 tgcatacgca gatacctgca tagcaataca atataccata atgtataaaa catgcctact
     1501 cattcctaac aatataagaa ttacttattt tgggcactta ccttaatctt tacccgcaac
     1561 ttaccatcgg cacttgttgt ttggtcaatt tctttagaaa taatatgagg atttcgggga
     1621 accaaagcct ctttaaactt aaattgttca ctaccaccat atattcccac ctcggtacca
     1681 tcggtagcat acgtagatgc tgttgtatat aaataaggac gaatatgata attgcttcca
     1741 tagtttattg tataaggggt aatatcattt acaaaaatag aatctgaatg cacttgtaaa
     1801 ttattactat atgtttgcgt tcctccgctt aaagcaaaat ttaaaatatt attttgaaaa
     1861 acattattat tacctgacaa aaacgaagcg gcatatccaa atgatatttt aaaaatatta
     1921 tttttaatca aacaatccga aataccatta agtaaaaccg accaactacc ggcagatgtt
     1981 attatattat tatgttcaat caatgcattt gaaacattat acatatgatg atgaaatata
     2041 ttattagcaa aaattaaggc atcggcattt tcaactgaca cagggccttt aataatattt
     2101 tgattaatta tagttcttag attatttcgg gaactcccgg taatattaag ttcattaaac
     2161 tcatttcggc gaattagtac atctttgatt gcagtattag cagcaaaatt aatattacca
     2221 tctatataca atccttctat tatagtacta tcagaaccgg ctgccaacga aagtgttgaa
     2281 gtaagtcttg atatgccggt ggcagacgta gaatcgggaa atactcctgt gcctataaca
     2341 actaattttt ttgaaatagt agcagaagta aaaaaaccac ctgacaaata aatagtatca
     2401 ccatttacag cagctgtatt tgcagcaaca aaagcattta ttccgctata tgcagtaaca
     2461 gtacccgcat gatgaagcat tacacgtgtt tgagcatatc cggttgaaaa accgaaacaa
     2521 accaatgcaa aaggaatggc ataacataag atttttttca taacagtatg tattaatatt
     2581 ttttcaaatg tacataattt ttatatacat tatttatttt ataaaatatt attattcagt
     2641 ctactataaa catttaaaca caacaaaaaa gctctgtatt tacaagcaaa tacagagctt
     2701 tatattattt caattgtata atattatttg ggcaatccaa atgtatctac aacaaaatca
     2761 atgtctttgt cgcctctacc acttaaattt actaatatag acgattgtgg atattgttgt
     2821 gccaatttaa gagcatatgc tacagcatgt gaactttcta atgcaggaat tataccttct
     2881 aatcggctta attcataaaa cgcttctata cattctttgt catttattac atcataagta
     2941 acgcgtttaa tgtctttcaa catagaatgt tcggggccta cacccggata atctaaacca
     3001 cttgctacag aatacacagg cgatggttca cctttttcat cttgtaatgt ataacattta
     3061 aaaccatgaa ttatacccgg agtaccaaaa gtcatagttg ctgcatggtc gccaagattc
     3121 atagaacgac cacccggctc tacgccataa atagaaactt cttcatcttc taaaaatgcc
     3181 gaaaacaaac ccatagcatt acttccaccg ccaacacaag ctaccacgct atcaggtagt
     3241 tctcccgtca tttctaaata ttgttcacga gcttctatac ccactacacg ctgaaaatcg
     3301 cgaaccatca ttggaaatgg atgcggacca acaacagaac ctatacaata tatagaattt
     3361 acaggatctt taagatacgc ttcaaaagcg gaatctaccg cctcttttaa tgttttgagt
     3421 ccatgagtta caggaactac cgtagctcca agtattttca tacgcaccac attcggatgt
     3481 tcttttgcaa tatcaacctc gcccatatga atttcacatt ctaatccaaa atatgccgcc
     3541 gcagttgcaa gtgcaacacc atgttgtccg gcaccggttt ctgctattaa tttcttttta
     3601 cccaaatatt tagcaagcaa tgcttcaccc atacagtgat tcaatttatg agcacccgta
     3661 tgattcaaat cttcgcgttt taaatagatt ctaccaccgt atttttgtga taacctatta
     3721 caataatata ccggcgtagg tcttccctga taatgtttac gaatacttcg taactcattt
     3781 ataaaatcat gagacttact aatagtaaaa tacgcatcgg taattttttt catttcactc
     3841 tctaatacag gtggtataaa tgctccaccg tattcattaa aaaatccctc ttttgaagga
     3901 tacgttttaa aataattgtg tgacatataa tttttacatt tttagaaatt catgtaaaaa
     3961 tacaaaaaat ctaattcaac acactgtatt atataataaa gttaattata caactggatc
     4021 gtgcccatgt cctccccacg gatgacaacg caacaatcgt ttaataagca tataaccgcc
     4081 tttgaatact ccatattttt gaatagcttc tatgccatat tgcgagcatg taggtgtata
     4141 tctgcatgaa tttggcaacc atggagaaat aaaccactga tagagtttaa taggtattat
     4201 aagaatataa ttacaaacag tacacatatg ctattgtatt ttttgaatga ttttttgcag
     4261 agctttgttc tggatctggc ggactcgttc cttagtgacg c
//
LOCUS       DAOTOW010000092         4264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16807_length_4264_cov_3.372060, whole genome shotgun sequence.
ACCESSION   DAOTOW010000092 DAOTOW010000000
VERSION     DAOTOW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16807_length_4264_cov_3.372060"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="P5243_02390"
     CDS             complement(<1..1534)
                     /locus_tag="P5243_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS18326.1"
                     /translation="MKTILFISAAITMMFHISFAQNITKYNGLPAEDKYVVFKYKPLL
                     KSMPANNEYSQAFQEFMQAIGAQVPQQKFPLAKMPQNCDSCVDITRIYDFYYTQDYPL
                     EKVIAYLRSMPFIEYAEPNYIHELLYVPNDPEVPFLYHLNTCYVYDAWNVHKGDTNTV
                     IAIVDAGVAIAHTDLVNQIKYNYADPINGFDDDYDGYIDNFRGWDFAEKDNNPDIGVS
                     DHGTYVAGISNAEVHNSFATAGVGFKCKFLPIKVATDAGIITRGYEGIVYAANQGAHI
                     INCSWGSAGSYTKYGLDVINYATFNCNSLVVGAAGNSNNTGVFYPASYNNALSVGSTQ
                     EGSKKWYTSATKGSNWNYYVDVSAPSVGYKTTAKNNGTTFVYGGTSFAAPIVSGVAAL
                     IKSKYPSYTPLDITERIRTTANDIYGITENLPYVDLLGSGEVNAYEALTNTSKPSIRV
                     QSFSVTNRSGQYMYYAGDTLELQISFKNYLASATNMYITISCETSIIAPLESNVLIPV
                     FDS"
     gene            complement(1539..2294)
                     /locus_tag="P5243_02395"
     CDS             complement(1539..2294)
                     /locus_tag="P5243_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS18327.1"
                     /translation="MIELQHISKSFKDFKVLDDISTTFQEGKVNFTIGKSGSGKTVLL
                     KSIVGLHEIDEGRILFHNRDFTNMDLKQRKEIRKEIGMVFQGGALFDSATLEENVMFP
                     LKMFSNLSYDECRERANFCLKRVEVINKNHLYPAEISGGMQKRVAIARAIALNPKYLF
                     CDEPNSGLDPITSIVIDNLIKEIILEFNITAVINTHDMNSVMEIADNIIFIHQGKKWW
                     QGNKEEIWRTDNEELNDLIFASELTKKLKKFYV"
     gene            complement(2296..3042)
                     /locus_tag="P5243_02400"
     CDS             complement(2296..3042)
                     /locus_tag="P5243_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS18328.1"
                     /translation="MSLYNFFYYIGRYFMLMRRVVTKPDDSKLFFANVSREIIVIGYN
                     SITIVAIISLFMGAVLTLQTAYNMTNPLYPDYLIGLGTRDSIFLEFSSTIVGLILAGK
                     VGSNIASEIGTMRVTQQIDALEIMGVNSANFLILPKIVAALCTFPFLCLISMIVGISG
                     GWIAGELSGEVPTSQFVYGIQYLFIPYYILYSLIKISFFGVIITSISSYFGYFTNGGA
                     LEVGHSSTKAVVNSTLVILIFNLILTSILL"
     gene            complement(3179..>4264)
                     /gene="purB"
                     /locus_tag="P5243_02405"
     CDS             complement(3179..>4264)
                     /gene="purB"
                     /locus_tag="P5243_02405"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRS18329.1"
                     /translation="TTNHDVKAVEYFIKEKFDVLGLTQYKEFIHFGLTSQDVNNTAVP
                     LSLKEAMYQVMYPAIEQVLSKLSELVEEYKDIPMLARTHGQPASPTRLGKEIQVFVER
                     AHIQLEQLQAIPYSAKFGGATGNFNAHHVAYPSIDWQVFGNNFVNDMLGLHRSQTTTQ
                     IEHYDNLASMCDAFKRINTILIDLCRDIWQYISMNYFKQKIQKNEVGSSAMPHKVNPI
                     DFENAEGNLGIANALFEHLSAKLPISRLQRDLTDSTVLRNIGVPMAHTLIALLSLQKG
                     LGKLLINETALHADLEANWAVVSEGIQTILRREGYPNPYEALKELTRTNSVINQVSIA
                     AFIDTLQVSEIVKNELRAITPYNYTGI"
BASE COUNT         1385 a          730 c          659 g         1490 t
ORIGIN      
        1 cgctgtcgaa tacgggaatt aaaacattag attctaaagg agctataatt gacgtttcac
       61 atgatatagt aatatacata ttggtagcag atgccaaata atttttgaaa cttatttgta
      121 attccaatgt atcgcctgca taatacatat actgaccact gcgattggta acactaaaac
      181 tttgaacccg aatcgaaggt tttgatgtat tggttaaggc ctcatatgca tttacttcac
      241 cactgccaag taaatcaaca tagggtaaat tttcagttat accatatatg tcatttgctg
      301 tggttctaat gcgttctgtt atatcgagcg gagtatacga tgggtattta cttttaatta
      361 gtgcagcaac tcccgaaaca ataggagcag caaatgatgt tcctccataa acaaacgtag
      421 ttccattgtt ttttgccgta gttttgtaac ctacagaagg agccgaaaca tcaacatagt
      481 aattccaatt actcccttta gtagcagagg tataccattt tttgctacct tcttgtgttg
      541 accctacact caatgcattg ttgtatgatg caggataaaa tacgccggta ttattgctgt
      601 tacctgccgc tcctaccact aatgaattac agttgaaagt agcataatta attacatcta
      661 gtccatattt agtatatgat ccggcacttc cccacgagca atttattatg tgtgctcctt
      721 gatttgcagc atatactatt ccttcgtatc cacgagtaat aattccggca tcggttgcta
      781 ccttaatagg taaaaattta cacttgaatc ctacacctgc agttgcaaat gaattatgta
      841 cttcggcatt tgaaatacct gctacatacg tgccatgatc tgaaactcct atgtcaggat
      901 tgttatcttt ttctgcaaaa tcccaacctc taaaattatc aatatatcca tcatagtcat
      961 catcaaatcc atttatagga tcggcataat tatatttgat ttgatttacc aaatcagtat
     1021 gagctatagc tacaccggca tctactattg ctattactgt gtttgtatct cctttgtgta
     1081 cattccatgc atcatacaca taacaagtat ttaaatgata taaaaatggc acttcggggt
     1141 catttggaac atacaataat tcatgaatat agtttggttc ggcatattct ataaaaggca
     1201 tgctccgtag atatgctata actttttcta acggataatc ttgagtgtag taaaaatcat
     1261 aaatacgtgt aatgtcaaca cagctatcac agttttgtgg catttttgcg agtggaaatt
     1321 tttgttgagg aacttgagct cctatagctt gcataaattc ttgaaacgct tgtgaatatt
     1381 cattatttgc aggcattgat tttaagaggg gtttgtattt aaaaacaaca tacttatctt
     1441 cggcaggtaa cccattatat tttgtaatat tttgcgcaaa acttatgtga aacatcatag
     1501 ttattgctgc acttataaat aaaattgttt tcatgggctt acacataaaa tttttttaat
     1561 ttttttgtta attccgatgc aaaaatcaaa tcattaagtt cttcattgtc ggtacgccaa
     1621 atttcttctt tattgccttg ccaccatttt ttaccttgat gaataaaaat tatgttatca
     1681 gcaatttcca ttaccgaatt catatcatgt gtatttataa cggcagttat attaaattcg
     1741 agaataattt cttttatgag attatcaata actattgatg taattggatc taaacctgaa
     1801 tttggttcat cacaaaataa atattttgga ttgagagcaa tagcccgagc aattgcaaca
     1861 cgtttttgca taccgccact aatttcggca ggatacaagt gatttttatt tataacttca
     1921 acacgtttta agcaaaaatt ggctcgttct ctgcattcat cgtaactcaa atttgagaac
     1981 attttaagag gaaacattac attttcttct aatgttgcag aatcaaataa tgctccgcct
     2041 tgaaatacca tacctatttc ttttctgatt tctttacgtt gtttcaaatc catattggta
     2101 aagtcacgat tgtgaaatag gattctaccc tcatcaattt catgtaaccc tactattgat
     2161 ttaagtaaca cggttttacc cgaaccactt ttaccgattg taaagttaac ctttccttct
     2221 tgaaaagtag tagatatatc gtcaagaact ttgaagtctt taaatgattt tgatatgtgt
     2281 tgtaattcta tcatactata ataaaataga agtaagaatt aaattaaaaa ttaatattac
     2341 caatgtactg tttactacag cttttgtact tgaatgtcca acttctaagg cgccaccatt
     2401 ggtaaagtat ccaaagtaac ttgaaataga agtaataatt acaccaaaaa atgatatttt
     2461 aattaaacta tacagtatat aatatggaat aaataaatat tgaattccat atacaaattg
     2521 acttgtaggt acttcaccgc ttaattctcc ggcaatccag ccgccgctta tacctactat
     2581 catacttatt aaacataaaa atggaaatgt gcaaagagca gctacaattt tgggtaaaat
     2641 gagaaagtta gccgaattta ctcccattat ttctaatgca tctatttgtt gagttacacg
     2701 catagttcct atttccgagg ctatattaga tccaactttt ccggctaaaa taaggccaac
     2761 tatggttgac gaaaattcaa gaaaaatact atcgcgggta cctaatccta ttaaataatc
     2821 ggggtagagc ggattggtca tattgtatgc tgtttgcaat gtaagaacag cacccataaa
     2881 taatgaaata attgcaacaa ttgtaattga attatatcca atcacaataa tttcgcgtga
     2941 tacatttgca aaaaatagtt ttgaatcgtc tggtttagtt actacccttc gcatgagcat
     3001 aaaataacga cctatataat aaaaaaagtt atataaactc atgaaaatca gatgtttaaa
     3061 aaataaaaaa ctttattata caatttttta taaaaataat atatttttca atagaaatac
     3121 agaattaaca gaattacttg tatttacttt gaaatagcat taaattacaa gtattctttt
     3181 aaattccggt ataattatac ggagttattg cacgtaattc attttttaca atttcactta
     3241 cttgcaatgt atctatgaaa gctgctatag aaacttgatt tattacacta tttgttcgtg
     3301 taagttcttt taatgcttca taaggatttg gatatccttc tcgtctaagt atggtttgaa
     3361 taccttctga tacaacagcc caatttgctt ctaaatcggc atgtaaggca gtttcattta
     3421 ttaataattt acctaatcct ttttgaagcg ataataatgc tataagtgta tgtgccatag
     3481 gaacacctat atttcgtaag actgttgagt cagtaaggtc gcgttgtaat cgtgaaatag
     3541 gaagttttgc tgaaagatgt tcaaataaag catttgctat tcctaggttt ccttctgcat
     3601 tttcaaagtc tataggatta actttatgtg gcatagccga tgacccgact tcattttttt
     3661 gaattttttg tttaaagtaa ttcatagaaa tatattgcca aatatctcgg cataaatcta
     3721 ttaaaattgt atttattcgt ttgaaagcat cacacatcga cgctaaatta tcgtaatgtt
     3781 caatttgtgt tgttgtttgc gaacgatgca gacctaacat gtcatttaca aaattattac
     3841 cgaatacttg ccaatcaata gaaggatatg ctacatgatg agcattaaaa ttaccggtag
     3901 ctccgccaaa cttagcagaa tagggaattg cttgcagttg ttctaattga atatgagcac
     3961 gttcaacaaa tacttgaatt tctttaccca aacgagtggg agatgccggt tgtccgtgag
     4021 tacgggctaa catgggaata tctttgtatt cttctactaa ttccgataat tttgaaagca
     4081 cttgttctat tgccgggtac attacttgat acattgcttc tttcaatgat agaggaacgg
     4141 cagtattatt tacatcttga gaagtaagcc cgaagtgaat aaattcttta tattgtgtta
     4201 atcccaacac atcaaatttc tctttaataa aatattctac agcctttaca tcatgattgg
     4261 tagt
//
LOCUS       DAOTOW010000093         4263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16812_length_4263_cov_3.363118, whole genome shotgun sequence.
ACCESSION   DAOTOW010000093 DAOTOW010000000
VERSION     DAOTOW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16812_length_4263_cov_3.363118"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="P5243_02410"
     CDS             complement(<1..238)
                     /locus_tag="P5243_02410"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRS18330.1"
                     /translation="MEQKIFGIPLLNSSDFIELIIRFSFNMFFIIVIVRFLYYAITKR
                     KDYLFTYILISTIVFLLCFLLSSVKMQIGFALGLF"
     gene            complement(247..1005)
                     /locus_tag="P5243_02415"
     CDS             complement(247..1005)
                     /locus_tag="P5243_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRS18331.1"
                     /translation="MDSTLINIINSFKPISLEEMDSVKLLVRNDYKFITTIPTLYAIL
                     KDVKDDYTILTINQNPFQEYKTIYFDTAEDSMYMSHQNGKLNRYKIRHRTYLSTNAEF
                     LEIKFKTNKGKTFKKRIPFPISKDLAEANDFITKNSPYSGEQLRHKSLVEYTRLTLVN
                     LAQGERATIDIDLQVTNCETNETVDFSHVCIIELKRDKSTSNSKLQSVLLKHRVFQRG
                     MSKYSIGTAAIYDIKKNRFKKKLRFIEKLKINSK"
     gene            complement(1009..1416)
                     /locus_tag="P5243_02420"
     CDS             complement(1009..1416)
                     /locus_tag="P5243_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18332.1"
                     /translation="MRIIILCISILCCNLYGSFAQSSKKQSGIKSKTEYEETFVKGSP
                     GQVIDNKTEYDKDGNVLKEYSYNKEGKLKQLVVYTYKNNKKETETWYGPDNKVIEKHV
                     YTYNARGDRIQKITYNGQDKMIKKKTYSYEYYQ"
     gene            complement(1429..2148)
                     /locus_tag="P5243_02425"
     CDS             complement(1429..2148)
                     /locus_tag="P5243_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18333.1"
                     /translation="MNKIGIIITSIFFIVSCAKQEGEGGIASIEGIMMVQKVNSMLKP
                     VGKPYRAKDKVYISYGTGTLQSDDANTSPEGKYVFNFLVPGNYSIFAYSDDTAKFNAI
                     EKIVIRKPVTIDTRKQKVTVDTLTIYKYVDFDDGSAQISGFVKQIEYYSGTTIPKDTI
                     SAQNADVFLTYGDNAEILDRIRSSYNGSFVFSNLIEGTYTIFVYSEQPYSKENLIVSK
                     TVTISSDNQKVQLAPMYVSNF"
     gene            complement(2296..3414)
                     /locus_tag="P5243_02430"
     CDS             complement(2296..3414)
                     /locus_tag="P5243_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS18334.1"
                     /translation="MYTIGFDAKRLFHNGTGLGNYSRSLVRSLYEEFPSYQYLLFTPS
                     ISYTNESFYFTEKFKVVTPKLFSKVGWRSRFMLYSLLEQNVDLYHGLSHELPFKIETT
                     DIKTVVTIHDVIYKFFPNDFPMIDKAVYDIKWKHACAVTHAIIATSEATKRDIIHFFG
                     VPEEKIHVVYQSCDPIFETIVSEKEKQHVCNTWNLPTDFILYVGAITERKNVLQLVQA
                     YNRVKNQVAIPLVIVGNGRQYLYKVKQYIEKESLQSQVKILTGVQNHDLPALYQSAQM
                     FIYPSKYEGFGIPILESLKSGTPVIASNTSSLPEVGGNAAMYIHPDSIESLCDAMVKV
                     YTDKDLRIDMIEKGFKHASNFSNTQFAYDTMSVYKKIV"
     gene            complement(3416..4135)
                     /locus_tag="P5243_02435"
     CDS             complement(3416..4135)
                     /locus_tag="P5243_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRS18335.1"
                     /translation="MQVSVFIPCFIDQFFPETGFNMIKLLEHVGCTVNYNPNQTCCGQ
                     PSFNSGYMKETITLATKFLQDFSGDDIIVSPGGSCTGFIKKQYASLFSEPEHIEQAQK
                     LATRMYDITDFLVHVIQKTQFGAVFNEKVTIHESCSALREYGLTNEPRLLLEQVQGIE
                     LIDLPDSKTCCGFGGTFSAKHTAISSAMVEQKVRNALSTGASYITSTEASCLMNIAGY
                     ISKNKLPIIPIHIVDILAKNL"
BASE COUNT         1488 a          663 c          538 g         1574 t
ORIGIN      
        1 caaataaccc aagagcaaat ccaatttgca tttttacact acttaacaaa aagcataata
       61 aaaatactat ggtagaaatt aatatatagg taaatagata atctttacgt tttgtaattg
      121 catagtataa aaatctaaca attactataa taaaaaacat attaaatgaa aatcggatga
      181 ttagttcaat aaaatcagaa ctatttaaaa gagggatgcc aaatattttt tgttccatac
      241 atataattat tttgagttaa tttttaattt ttcaataaat cgtaattttt tcttaaatct
      301 attcttttta atatcatata tagctgccgt tccaatagaa tatttgctca tacctctttg
      361 aaaaactcta tgttttaata acaccgattg caatttagag ttagatgttg atttatcacg
      421 tttaagttca ataatacaaa catgagaaaa atctactgtt tcattagttt cacaattagt
      481 tacttgtaaa tctatatcaa tagttgccct ttcaccttgc gctaaattaa ctaatgttaa
      541 tcgagtatat tctacaagag atttatgtcg taattgctct cctgaatatg gcgaattttt
      601 cgtaatgaaa tcatttgctt cggctaaatc tttggaaatt ggaaacggaa tacgtttttt
      661 aaatgttttg cctttatttg ttttaaattt aatttctaaa aattcagcat tagtagacaa
      721 ataagttcta tgtcttattt tatatctatt tagctttcca ttttgatgac tcatatacat
      781 tgagtcttct gccgtatcaa aataaatagt tttatattct tgaaatgggt tttgatttat
      841 agtaagaata gtataatcat cttttacatc ttttaaaatt gcatataatg taggaatagt
      901 tgtaataaat ttataatcat ttcttacaag caattttacc gaatccatct cctccaatga
      961 gattggttta aaagaattaa ttatattaat taaggtagaa tccattcttt actggtaata
     1021 ttcgtatgaa taggtttttt tctttatcat tttatcttga ccattatagg taattttttg
     1081 aattctatca cctctggcat tataggtata tacatgtttt tctataactt tattatcagg
     1141 tccataccaa gtttctgtct cctttttgtt atttttataa gtatatacta caagttgttt
     1201 taactttcct tctttattat atgaatattc tttcaaaaca ttcccgtctt tatcgtactc
     1261 tgttttatta tcaattactt gccccgggct tcctttaaca aaggtttctt catactctgt
     1321 ttttgacttt attcctgatt gttttttaga actttgtgca aaagatccat acaaattaca
     1381 acaaagtatg cttatgcata atataattat tctcatagca atatattttt aaaaatttga
     1441 aacatacata ggagccaatt gtactttttg attatcagaa gaaatggtaa ctgttttact
     1501 tacaattaaa ttttcttttg aatatggctg ttcactatat acaaaaattg tataagtacc
     1561 ttctattaaa ttagaaaata caaaactacc attataggat gaacgaattc tatctaaaat
     1621 ttctgcgtta tccccataag tcaaaaaaac atcagcattt tgagccgaaa tagtatcttt
     1681 aggtatggtt gtgccagaat aatattcaat ttgttttaca aatccgctta tttgagcaga
     1741 cccatcatca aaatcaacat atttataaat tgtaagtgtg tcaacagtaa ctttttgttt
     1801 tcttgtatca atagttaccg gttttctgat tactattttt tcaattgcat taaatttagc
     1861 tgtatcatcg gaatatgcaa aaatagaata attaccgggc actaaaaaat taaaaacata
     1921 tttaccttcc ggagaagtat tggcatcatc actttgcaat gtgccggtac catatgatat
     1981 atatacctta tcttttgctc tatatggctt accaacaggt ttaagcatag aatttacttt
     2041 ttgcaccatc ataatacctt ctattgaagc aattccacct tctccttctt gtttagcaca
     2101 tgaaacaata aaaaatatag atgttataat tattcctatt ttattcatac ttttttaatt
     2161 aaaagaaaca actacaaaaa tacatattgt ttttaatttg ataacaattt cataacaata
     2221 aaaaaatgta taaccctacg aattttatat gcaaaaacaa aaaacaagta ttaatacaca
     2281 gatgattata aatacttata caatcttttt atatacactc attgtatcat aagcaaactg
     2341 agtatttgaa aaatttgatg catgcttaaa tcctttctct atcatatcaa tacgcaaatc
     2401 tttatctgta tacactttca ccatagcatc acataaactt tcaattgaat caggatgaat
     2461 atacattgct gcattaccac ctacctctgg taatgaagac gtatttgatg caattaccgg
     2521 agtaccactt ttaagcgact ctaaaattgg tatgccaaat ccctcatatt ttgatgggta
     2581 aataaacatt tgagcagact gatagagtgc cggtaaatca tgattttgca cccctgttag
     2641 tatttttact tgagactgta atgattcttt ttctatatat tgcttaactt tatacaaata
     2701 ttgtcttcca ttaccaacaa taactagtgg tattgcgact tgatttttaa ctctattata
     2761 tgcctgtacc aattgaagta cattttttcg ttcagtaata gctcccacat ataaaataaa
     2821 atctgttggc aaattccatg tattgcaaac gtgttgtttt tctttttcac ttacaatagt
     2881 ttcaaatata gggtcgcatg attgatatac tacatgtatt ttctcttcag gaacaccaaa
     2941 aaaatgaata atatctcgct ttgttgcttc actggttgca attatggcat gagtaacagc
     3001 acatgcatgt ttccatttta tgtcatacac tgctttatca atcataggga agtcatttgg
     3061 aaaaaactta tatattacat catgtattgt aacaactgtt tttatatctg ttgtttctat
     3121 tttaaaaggt aattcatgac tcaatccatg atacaaatca acattctgtt ccaacaaaga
     3181 atataacata aatcgcgaac gccacccaac ttttgaaaat aatttaggtg ttacaacttt
     3241 aaatttttca gtaaaataaa aactttcatt ggtatatgaa atacttgggg taaaaagtaa
     3301 atattgatat gaaggaaatt cctcgtataa acttcgaacc aaacttcggc tataatttcc
     3361 taaacctgtt ccgttgtgaa acaatcgttt tgcatcaaat ccaattgtat acatcttata
     3421 gatttttagc taaaatatca actatatgaa ttggtataat tggcaatttg tttttagaaa
     3481 tatatcctgc tatattcatt aaacaagaag cttctgtgga ggtaatatat gaggctccgg
     3541 ttgatagggc attacgaact ttttgttcaa ccatagcaga tgatattgca gtatgttttg
     3601 cagaaaatgt accaccaaaa ccacaacacg ttttagaatc aggtaaatca attaattcta
     3661 taccttgaac ttgctccaac aataaacgag gttcatttgt aagtccatat tcacgaaggg
     3721 cagaacaaga ttcatgaata gttacttttt cattaaatac agctccaaat tgagtttttt
     3781 gtataacatg tactaaaaag tcagtaatat catacattct agttgcaagt ttttgagctt
     3841 gttctatatg ttcaggttca gaaaataatg aagcatattg ttttttaata aaacctgtac
     3901 aagaccctcc gggactaaca ataatatcat cccccgaaaa atcctgtaaa aatttagttg
     3961 ccaaagtaat tgtttctttc atataaccac tattgaacga aggttgccca caacatgttt
     4021 gattaggatt gtagtttaca gtacagccta catgttctaa taatttaatc atgttaaaac
     4081 ctgtttcggg gaaaaactga tcaataaaac aaggaataaa tacagaaact tgcataataa
     4141 aaattttaaa tacaaaaata cagaaacagt ttcatttttt taagaaaaga acttgtaatt
     4201 acataaaatg ttgtaaaaat gtgtaaattt gatagataga aatacgacaa aataacaaaa
     4261 aat
//
LOCUS       DAOTOW010000094         4249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16893_length_4249_cov_2.894850, whole genome shotgun sequence.
ACCESSION   DAOTOW010000094 DAOTOW010000000
VERSION     DAOTOW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16893_length_4249_cov_2.894850"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="P5243_02440"
     CDS             <1..1535
                     /locus_tag="P5243_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRS18336.1"
                     /translation="TNHLYKVMLFFRKNLEISISRVPFVFITTALLCGIVITSLYNIS
                     ISQTLITFFILNTCLCIALYYLQKPYQLVVYFVWFVLFGMLLTIIHNSKYKAIIGKHT
                     VTAQITSALSEKSKTYSAEITMYENSIHKAIIYIAKDTASQKLMYGDIIECTAIFKPI
                     VQDTTQLFNYQAYTANKHIFSTAYIGGNMWKKIGYTSSIQSISLAIQHNILHILHDSN
                     LQTQNFELLAALIFGNKTYLQTDTKQEFMKAGAMHILAVSGLHVGIIAIIIMKVLQIL
                     PIYKRTWIKTLLCVICIWIYACITGLSPSVTRAACMFSLLCTSIILNRKVSTYNTLAA
                     AACIMLILNPLTLFDIGFQLSYTAVIGIVYFGNKIQSIFPHIQISTIQYIWSIIAVSI
                     AVQITTLPISLYYFGFLPTYSLLTNICAIPIAFVLLSGIIICICCYSIPYISTYIFSC
                     IDFVASYLQHIVYVISQLPHSSIQIHISLQKVIILYVLIVFCIVIIEQLQLRRIQKNS
                     AW"
     gene            complement(1497..2339)
                     /locus_tag="P5243_02445"
     CDS             complement(1497..2339)
                     /locus_tag="P5243_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRS18337.1"
                     /translation="MKLQIAFSSCPNDTYIFDALVHKKIDTHGIDFEYTIADVEELNQ
                     KALSATFHITKISYFAYAHCFQIYQILDAGSALGYANGPLFICQKGKEQTINAASTIM
                     LPGKYTTAHVLFCMAYPEYINKKFAVFYEIEQAITNGTADAGVIIHENRFTYAQRGFS
                     CICDLGTAWEQQTNMPIPLGAIVIHRSLPEDIKQTVNILIRKSIEFARVNPYESQPFI
                     DTNAQEMESDIKKQHINLFVNDFSIDLGLVGKQAISTLYAKAFEYKLIPELPRTIFLN
                     SPQL"
     gene            complement(2348..4054)
                     /locus_tag="P5243_02450"
     CDS             complement(2348..4054)
                     /locus_tag="P5243_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HRS18338.1"
                     /translation="MDSAINIYQVFTRLFNNKNQTNKEFGTIEENGCTKFNDITSTAL
                     EQIKKFGSTHIWYTGIIRHATTTDYSKYGLSTDNPYVVKGRAGSPYAIKDYFDVHPDF
                     AVSVKNRMHEFEKLVERTHDAGLKCIIDFVPNHVSREYKSENLPAGCSALGSYDDTSL
                     AFSPSNNFYYIPNQELHLPEEINFPYTHNAPKYNEIPAKVTGNDVFSAYPSINDWYET
                     VKLNYGIDYQNLWQEHFEEIPDTWVRMLEVLEFWTKKGVDGFRCDMAEMVPVRFWGWV
                     IPQIKQLNPEIIFIAEVYNPEEYRNYLSRGKFDYLYDKVGLYDILRLIIEGVGSAKYV
                     SDCWKTHDDITSKMLRFLENHDEQRIASRFFANNAEAAKASMILAGTMDRGPLMIYFG
                     QEIGEKGMDKEGFSGIDGRTSIFDYWSLVEYNKWVNGGKFDGAKLSDEQKELRDFYKS
                     LNWLRLNHEAIHSGLFYDLMWKNYNGNLDSDKIFAFLRYTSNQKMLIIINFDMHQTHT
                     FRLFIGEHALETMNFKKEGKMKLKEVFEADFTDEFPMQEVLELGIPFSILPNSALVFE
                     LS"
BASE COUNT         1454 a          738 c          554 g         1503 t
ORIGIN      
        1 acacaaacca tttgtacaaa gtgatgcttt tttttcgtaa aaaccttgaa atttcaattt
       61 cacgagttcc ttttgtattt ataaccacag ccctactttg tggtattgta ataacttctt
      121 tgtataatat atctattagt caaacactta ttacattttt tattctcaac acgtgtcttt
      181 gtattgcttt gtattattta caaaagccct atcaacttgt agtatatttt gtatggtttg
      241 tgttatttgg aatgttactt accattattc acaattcaaa atataaagca ataataggta
      301 aacatactgt tacagctcaa attacatcag ctctttctga aaaatcaaaa acatattctg
      361 cagaaattac tatgtacgaa aatagtatac ataaagcaat aatatacata gcaaaagaca
      421 ctgcttcgca aaaacttatg tatggtgata ttatagaatg cactgctatt tttaaaccta
      481 ttgtacaaga cacaacacaa ctcttcaatt atcaagcata tactgccaat aaacacatct
      541 tttctacagc atatattggc ggcaatatgt ggaaaaaaat tggttataca tcttctattc
      601 aaagcatcag tttagctatt caacataata tacttcacat attacatgat tcaaatttgc
      661 aaacacaaaa ctttgaacta cttgcagctc ttatttttgg aaacaaaacc tacttacaaa
      721 cagacacaaa acaagaattt atgaaagccg gtgctatgca tattttagcc gtttctggtt
      781 tacatgtagg aattatagca attataataa tgaaagtatt gcaaatacta cctatttaca
      841 aacgaacatg gattaaaacc ttactttgtg ttatttgcat ttggatatat gcatgtatta
      901 caggcttaag tccttcggta acacgagctg catgtatgtt ttcattactc tgcacttcaa
      961 taattcttaa tcgaaaagtt tcaacataca atacattagc tgccgcagct tgcataatgc
     1021 tcatactaaa tcctctaaca ctttttgaca ttggttttca attatcatat actgctgtta
     1081 ttggcattgt atattttgga aataaaatcc aatcaatatt tcctcatata caaatcagca
     1141 ctattcaata tatatggagc ataatagccg taagtatagc agtacaaatt accactctac
     1201 caatctcttt gtattatttt ggatttttac caacatatag tttacttacc aatatatgtg
     1261 ctattccaat agcatttgtt ttattatcgg gtattattat atgtatttgt tgttattcaa
     1321 ttccatacat aagcacttat atattttcat gtattgactt tgtagcttct tatttacaac
     1381 atatagtata cgtaatttct caattacctc attcttcaat acaaatacat atttctttac
     1441 aaaaagttat tattctgtat gtgcttattg tattttgtat tgttattatt gaacaattac
     1501 aactgaggag aattcaaaaa aatagtgcgt ggtaattctg gaattaattt gtattcaaag
     1561 gcttttgcat acaaagtaga aattgcttgt tttccaacca accctaaatc tatactaaaa
     1621 tcatttacaa ataaattaat atgctgtttt tttatgtcac tttccatttc ttgagcattt
     1681 gtatcaataa acggttgtga ttcataagga ttcacacgag caaattcaat acttttgcga
     1741 ataagaatat tgactgtttg tttaatatct tcgggtaagg aacgatgtat tacaatagca
     1801 ccgagaggta taggcatatt tgtttgttgt tcccatgctg tacctaaatc acatatacat
     1861 gaaaatccac gctgtgcgta agtaaatcta ttttcatgta taataacacc ggcatcggcg
     1921 gtgccatttg ttatagcttg ttctatttca taaaatacag caaatttttt attgatatac
     1981 tcaggatatg ccatacaaaa taacacatga gctgtagtat attttccggg taacataata
     2041 gtagacgcag catttattgt ctgttccttt cctttttgac atataaacaa aggaccatta
     2101 gcatatccta aagcactgcc ggcatctaaa atttgataga tttggaaaca atgagcataa
     2161 gcaaaataac ttattttggt tatatggaaa gttgctgata atgccttttg atttaattct
     2221 tctacatcag caatagtata ttcaaaatca attccatgtg tatcaatttt tttatgtact
     2281 aaagcatcaa atatatacgt atcgtttgga cacgatgaaa atgcaatttg taatttcatt
     2341 gaaaaactta tgataactca aatactaagg cagaatttgg aagaattgaa aacggaatac
     2401 ccaattctaa aacttcctgc ataggaaact catcagtaaa atcagcttca aacacttctt
     2461 ttagcttcat ttttccttcc tttttaaaat tcatagtttc taatgcatgt tcacctataa
     2521 acaatctaaa agtatgagtt tgatgcatat caaaattaat aattataagc atcttttgat
     2581 ttgacgtata tcgtaagaaa gcaaaaattt tatcggaatc taaattacca ttatagtttt
     2641 tccacattaa atcataaaac aaaccactat gtatagcttc atgatttaat cgcaaccaat
     2701 tcaaagattt ataaaaatca cgaagttctt tttgttcatc agataattta gcaccatcaa
     2761 atttgccccc gtttacccat ttattgtatt ctaccaaact ccagtaatca aaaatacttg
     2821 ttcttccatc tattccacta aaaccttctt tatccatacc tttttcgcca atttcttgac
     2881 caaaatatat cataagtgga cctctatcca tagtgccggc caatatcatt gaagccttag
     2941 ctgcttcggc attattggca aaaaaacgag aagctattcg ttgttcatca tgattttcga
     3001 gaaaccgaag catttttgat gttatatcat catgcgtttt ccagcaatca ctaacatatt
     3061 tagcagagcc tactccttca attatcaaac gtaaaatatc atataatccg actttatcat
     3121 ataaataatc aaattttcca cgagataagt aatttcgata ttcttcagga ttatatactt
     3181 cggcaataaa aataatttca ggattcaatt gtttaatttg aggaatcacc cagccccaaa
     3241 aacgaacagg aaccatttct gccatatcac aacggaagcc atcaacacct ttttttgtcc
     3301 aaaattcaag aacttctaac attctgaccc acgtatcagg aatttcttca aaatgctctt
     3361 gccacaaatt ttgataatct attccataat ttaatttaac cgtttcgtac caatcgttaa
     3421 tagatggata tgcagaaaac acatcgtttc cggtaacttt agccggtatt tcattatatt
     3481 taggagcatt gtgcgtatat ggaaaattta tttcttctgg taaatgtaat tcctgattcg
     3541 gaatataata aaaattatta cttggtgaaa atgctaaaga cgtatcatca taacttccta
     3601 aagcagagca ccctgcaggt aaattttctg atttatattc tcgcgataca tgattaggaa
     3661 caaaatcaat tatacatttt aatccggcat catgagtacg ttctactaac ttttcaaact
     3721 catgcattct attttttaca cttacagcaa aatcaggatg cacatcaaaa taatctttta
     3781 tagcatatgg agaacctgct cttcctttta ccacatatgg attgtcggta cttaaaccat
     3841 atttagaata atctgtagta gttgcatgtc taataatacc ggtataccaa atatgagttg
     3901 atccaaattt ttttatttgt tcaagagcag ttgatgtaat atcattaaac ttagtgcaac
     3961 cattttcttc aattgttcca aattctttgt ttgtttggtt tttattatta aaaagacgag
     4021 taaaaacctg atatatatta attgcagaat ccattgtacc taattttacc tccaaatgta
     4081 cataatatta taaaaatttg aaagttttta aagaaataaa aaaaaataaa aaaatacatg
     4141 aaagtatttt gcgaataaaa aaaatgtcta tatttgcagc acaaaattca ggttcggtag
     4201 ttcagttggt tagaatacat gcctgtcacg catggggtcg cgggttcga
//
LOCUS       DAOTOW010000095         4245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16913_length_4245_cov_2.821480, whole genome shotgun sequence.
ACCESSION   DAOTOW010000095 DAOTOW010000000
VERSION     DAOTOW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16913_length_4245_cov_2.821480"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2531
                     /locus_tag="P5243_02455"
     CDS             <1..2531
                     /locus_tag="P5243_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS18339.1"
                     /translation="VSGCSPLQINIQNATIGATQYMWDFGNSVQSPSLIPNQTYINTE
                     STVKVTHIQLRASNEYTCTDTASQAITVYPQVIADFFPNVSGCSPLPIQFTNISKNGT
                     SYSWNFGDGKFSTETNPVNTFINEGAKDTTYTITLIGSNHFSCADTIQKDIHVYPGIQ
                     AEFTVSSSVACSPMNTQITSKTKGAVYTEWVINNVSGQHIQEQSFMYTAQNKTTLPQV
                     HVVELRVRNAYSCSNTQVQTLQIYPQVTADFTVSKQKGCSPLDVNFINTSSGAISYFW
                     IFGDNSDASNANPGHTYLNYDTVAQNFPVQLVATSAYGCVDSSGITNLTVYPQPSAMF
                     HVQSHAGCSPFTVTITDESVGATHYSWDFGSGNLSETFKSVHTFVNTTADVKTPKISL
                     TVYNDYMCSDFTQEPLMVYPEVVADFIAPQSGCNPLSVTFQNTSLYAQSYLWNFGKGI
                     TSTAKDPMYSFTNSSLRDSLYTVSLRAMNVYGCRDSIEKQLTIYATPQASFLPSASQV
                     FMPDAVVSFENKTKGEWQYAWHFGDGVNSTQANPEAHTYTSPGSKTVTLIAYSNVCSD
                     TIQQIIKVLGPPVIARYDTSYAGCSPLEVTFTNKSMNATEYIWDFGDGTTSTEVHPTH
                     TFVVPGTYIIQLTAKNEEAEDISRAHTVTVYRNPKADFSIAPEVVYLPDAEISTYNHS
                     ADVQDVTWYFGVDDISHDFQPTYKFTEEGEFNIALTVTSRDGCKDSLMIPKAIRVLHT
                     CDLKFPNAFTPVLQESDGYYDSSVPETENNIFHPIFINIVEYELQIFNRWGEIVFESK
                     ELEKGWNGFYKKELSKSDVYVWKCTAQCLGGKTIQKVGNVTLLR"
     gene            2537..3577
                     /locus_tag="P5243_02460"
     CDS             2537..3577
                     /locus_tag="P5243_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRS18340.1"
                     /translation="MYKIIVVIILLLSMKHVQAQDPQFSHLYAMPLHMGPSFAGATGG
                     TRAAINGRNQWTAVKQEYNTVSIMLDHYFYGTRNGAGIVLLRDQAGAGKLINSTIQAQ
                     YAYSININKFIALRPGIQMSFAHRTIDYSKIVFGDQLDFIENRPNTIEPSLINSKKYI
                     DFGASLLGMYNNVWFGGTLDHLVTPNQSLKNETSEVPLRLHIYSGARFKVQTTFNKKR
                     KRENVYVMLHYNQQENFKQALYGSYWEHNNTIFGFWYRGMPYAKAYNNYINSDALVYM
                     LGFKYYGLQFLYSYDFTLSKLTANTAGSHEITITWNWNSASEKLQKSKMKVVPCPMQE
                     NSRSRQHNQHNM"
     gene            3714..>4245
                     /locus_tag="P5243_02465"
     CDS             3714..>4245
                     /locus_tag="P5243_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS18341.1"
                     /translation="MKLHILLIIFLFSYNLVFGQTQYSLQHFSLSDGISDSYITGITE
                     DTTGKIWISTYFGVNAFTGSSFEHYVAEDAQYNKLMRNDINCIYTDISKKIWIGGQNG
                     TIQTYNSIYDRFDNVSLNLITIEEYPSFYSFYQKNGTELYGLSSKGLYRFSKYSGKFH
                     KAFREVHELYSTHVTCM"
BASE COUNT         1441 a          657 c          743 g         1404 t
ORIGIN      
        1 ctgtttcggg ttgtagtcct ttacagataa atattcaaaa tgcaactata ggagcaactc
       61 aatatatgtg ggattttggt aattctgttc aatcgccatc gctcatacca aaccaaacat
      121 atataaatac cgaatctacg gtaaaagtta ctcatataca attgcgtgca tctaatgaat
      181 atacatgtac tgataccgca tcgcaggcta ttactgtata tccgcaggtt atagcagatt
      241 tttttccaaa tgtttcaggg tgtagtccat taccaataca atttaccaat atatcaaaaa
      301 atggaacttc atattcatgg aattttggtg atggtaaatt ttcaacagaa acaaatcctg
      361 taaatacgtt tataaatgaa ggagctaaag atacaacgta tacgattact cttattggca
      421 gcaatcattt ttcatgtgct gatactatac aaaaagatat acatgtatat ccgggaattc
      481 aagcagaatt tacagtgagt agttctgtag catgttctcc tatgaatacc cagattactt
      541 ctaaaactaa aggggcagta tataccgaat gggtaataaa taatgtaagt ggacaacata
      601 tacaagagca atcgtttatg tatacagccc aaaataaaac aacattacct caagtacatg
      661 ttgtagaatt acgtgttaga aatgcatatt catgtagtaa tacgcaggtg caaacgttac
      721 aaatataccc tcaagtaaca gcagatttta ctgtttcaaa acaaaaaggc tgtagtccgc
      781 tcgatgtaaa ttttataaat acatcaagtg gtgctatttc atatttttgg atatttggcg
      841 ataactcaga tgcttctaat gcaaatccgg gacatacgta tttgaattat gacacagttg
      901 ctcaaaattt cccggtacaa ctagtagcaa cttcagcata tgggtgtgtt gattcttcgg
      961 gcataaccaa tcttacagtg tatccgcaac cttcggcaat gtttcatgta caatcacatg
     1021 ccggttgttc tccttttacc gttactatta cagatgaatc ggttggagct acacattatt
     1081 catgggattt tggatcgggt aatctttcag aaacgtttaa atctgttcat acgtttgtaa
     1141 ataccacagc cgatgtaaaa acaccaaaaa tttcattgac cgtgtataat gattacatgt
     1201 gttctgactt tacacaagaa cccttaatgg tatatcctga agttgttgca gattttattg
     1261 cgccacagtc gggttgcaat cctctctcgg ttacattcca gaatacctct ttatatgctc
     1321 aatcatattt atggaatttt ggaaaaggaa taacatctac tgcaaaagac cctatgtata
     1381 gttttacaaa ttcttcacta agagattctc tatataccgt ttcgttgcga gctatgaatg
     1441 tatatggatg tagagattct attgaaaaac aattgacaat atatgcaacc ccccaagcat
     1501 catttttgcc aagtgcctca caagtattta tgcctgatgc cgtagtttct ttcgaaaata
     1561 aaacaaaagg cgaatggcaa tatgcatggc actttggcga tggtgtaaac agtacccaag
     1621 ctaatcctga agctcatacg tatactagtc cgggtagcaa aacagtaacc ttaatagctt
     1681 atagtaatgt gtgtagtgat actattcagc aaattataaa agtgttaggt cctcctgtta
     1741 ttgcaagata tgatacttca tatgcaggtt gtagtccgct tgaggttacc tttacaaata
     1801 agtcaatgaa tgcaactgaa tatatatggg attttggcga tggaactaca tcaaccgaag
     1861 tgcatccaac acatacgttt gttgttcccg gaacttatat catacaattg acggcaaaaa
     1921 atgaagaagc agaggatata tcgagggcac atactgttac tgtgtatcga aatcctaaag
     1981 ctgatttcag tattgcaccc gaagttgtgt atttgcctga tgctgaaatt tctacatata
     2041 atcattctgc tgatgttcaa gatgttactt ggtattttgg tgtcgacgat atttcacatg
     2101 attttcaacc aacgtacaaa tttactgaag aaggtgaatt taatatagca cttacggtaa
     2161 catcgcgtga tggttgtaaa gattctttaa tgataccgaa agctattcga gtacttcata
     2221 cttgtgattt gaagtttcct aatgctttta cgcctgtttt gcaagagtca gatggatatt
     2281 atgatagttc tgttccagaa acagaaaata atatttttca tcctattttc ataaatatag
     2341 ttgaatatga attacaaatt tttaatagat ggggcgaaat tgtttttgaa agtaaagaat
     2401 tagaaaaagg atggaatgga ttttataaaa aagagctttc gaaatcagat gtgtatgtgt
     2461 ggaaatgtac cgcacaatgc ttgggtggaa aaactataca aaaagttgga aatgttacat
     2521 tattgcgata agaaatatgt ataaaattat tgttgttata atcctattat tgagcatgaa
     2581 acatgtgcaa gcgcaagatc cgcaattttc acatttgtat gctatgcctt tgcatatggg
     2641 accttcgttt gccggagcta cgggaggtac acgagcggca ataaatggaa gaaatcaatg
     2701 gactgccgta aagcaagaat ataatacagt atctattatg cttgatcatt atttttatgg
     2761 tactcgcaat ggtgccggca ttgttttatt gcgtgaccaa gcaggggcag gaaaacttat
     2821 aaattcaaca attcaagcac aatatgcata ttcaattaat attaataaat ttattgcgtt
     2881 acgtcccggt attcaaatga gttttgcaca cagaactatt gattattcaa aaatagtatt
     2941 tggcgatcaa cttgatttta ttgaaaatcg tccaaatact attgaaccat ctcttataaa
     3001 tagtaaaaaa tatattgatt ttggagcttc attgttagga atgtataata atgtttggtt
     3061 tggaggtacg cttgatcatt tagtaactcc taatcaaagt ttaaaaaatg aaacttctga
     3121 agtgccgttg cggttgcata tttattcggg agcacggttt aaagtacaaa ctacttttaa
     3181 taaaaagcgt aaacgtgaaa atgtgtatgt aatgcttcat tataatcaac aagaaaattt
     3241 taaacaagca ttgtatggat cgtattggga acataataat actatttttg gattttggta
     3301 tagaggtatg ccatatgcta aagcgtataa taattatata aatagcgatg ctttggtata
     3361 tatgcttgga tttaaatatt atggtttgca atttttatac agttatgatt ttacactttc
     3421 aaaattaact gcaaatactg caggttctca tgaaataaca attacatgga attggaattc
     3481 ggcaagtgag aaattacaaa aaagtaaaat gaaggtagtt ccgtgtccca tgcaagaaaa
     3541 ttctcgttct cgtcaacata atcagcataa tatgtgattt ttttctttcc taacaaaact
     3601 tacgcaacat attgttattt caacaattag ttgtattttt actaattgtc attaaaacat
     3661 gaattatggt tataatatgt ttttatgata gatttgtata attaccaaaa actatgaaac
     3721 ttcatatttt acttattatt tttttgtttt catataattt ggtttttggt caaacccaat
     3781 attctttaca acatttttct ctttcagatg gaattagcga ttcatacatt acaggaataa
     3841 cagaagatac aacaggaaaa atttggattt caacatattt tggtgtaaat gcattcacgg
     3901 gttcatcatt tgaacattat gtggcagaag atgctcaata taataaatta atgcgaaatg
     3961 atataaattg tatatatacc gatatttcaa aaaaaatttg gattggaggt caaaacggaa
     4021 ctattcaaac gtataatagt atctatgata gatttgacaa tgtatcgctt aatttaataa
     4081 ccatagaaga atatccatcg ttttattcat tttatcaaaa aaacggaaca gaattgtatg
     4141 ggttaagtag taaagggtta tatagattca gtaaatattc aggaaaattt cataaagcat
     4201 ttcgtgaagt gcatgaattg tattcaacac atgttacttg tatga
//
LOCUS       DAOTOW010000096         4231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16972_length_4231_cov_3.486830, whole genome shotgun sequence.
ACCESSION   DAOTOW010000096 DAOTOW010000000
VERSION     DAOTOW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16972_length_4231_cov_3.486830"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1562
                     /gene="hisS"
                     /locus_tag="P5243_02470"
     CDS             198..1562
                     /gene="hisS"
                     /locus_tag="P5243_02470"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRS18342.1"
                     /translation="MAQKPSIPKGTRDFIPAEMSKRKYIFDTIQSIFTLYGYLPIETP
                     AMETMQTLMGKYGEEGDKLIFKILNSGEFNQGISADEYTQASSTALAHKICEKALRYD
                     LTVPFARFVVMHQNEIQFPFKRYQIQPVWRADRPQKGRYREFYQCDADIVGSNSLVNE
                     VELVQMVDAVFSKLGIRVCIKINNRKILAGIAEVIGESDKIIDITVAIDKLEKVGIEN
                     VNKELSDKGIADSTIAKLQPILELHGTTEQKLQQLQTILQSSEVGKQGIAEIEYICTN
                     ISHMSLSAEFELDLTLARGLNYYTGAIFEVKSKDVQIVSICGGGRYDNLTGIFGLPGV
                     SGVGISFGADRIYDVLQQLNAYPAQVNQTAKILFVCFGEPEQQQALLYAQSIRSKGIA
                     CEVYPDVAKMKKQMQYANDRGIPYVGIIGETELESHTIMLKNMITGTQEAVNIEQCIA
                     FLQK"
     gene            complement(1566..2981)
                     /locus_tag="P5243_02475"
     CDS             complement(1566..2981)
                     /locus_tag="P5243_02475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRS18343.1"
                     /translation="MNKTRQTLLYIISDFLAAAIAWTLFFLMRKKYIDVYFMKFSNEI
                     SFDKNYIYGLIIIPIMWVLLYYINGYYKDIFRKSRLIELIQTFILTLIGTLVIFFTLL
                     LDDNIYSYYSYYKSLLLLFSLHFFATYIPRAIITSITNYKIHKRILVFPTLLVGSNEK
                     AKQLYERMEAKKRSSGNAFIGFVNVIQQENYVMKQYMPHLGHIDNVKQVLKKHHVEEV
                     IIAIESKEHQYIEHILYKLEGIQATIKAIPDNCDIISGRVQMTSLHDEPLMVISRNPM
                     PAWQENTKRMIDVIVSIGVLILFLPLYIALAIGVKLSSPGPVFYRQIRIGKYGRPFRI
                     FKFRTMYVGAEKQGIALSKENDSRITPIGRFLRKTRLDEIPQFYNVLIGEMSLVGPRP
                     ERKYFIEQILPIAPQFIFLQRVRPGITSWGQVKFGYAKNVDEMIERLKYDIIYIENMS
                     LLLDIKILIYTIRTVLLGKGL"
     gene            complement(3095..4021)
                     /locus_tag="P5243_02480"
     CDS             complement(3095..4021)
                     /locus_tag="P5243_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRS18344.1"
                     /translation="MLRFTFYISIFLKKHVTTYPPAFVSIEPTNICNLQCPECPTGNK
                     TSTVSKGYISPQTFTSIVEACKPYTIAVNLYFQGEPFLHTQLVHMITQLHKARIITSI
                     STNAHFIDSKNAEAIVRSGLTHLIVSFDGYNQETYEIYRKQGSFEKVTQALTALQQAK
                     QTLQSHTPIIELQCLLFKHTQHHTQTISKLGKKYGADKVVFKTAQLYSAEHIDLLPDK
                     QYSRYTIKDNTIHIKQPLKNACWRMWSTCVISWQGTILPCCFDKNHTFAYGSLTDKSF
                     LEIITSEQTHRFKKTVHTKRSSIKMCNNCSTP"
BASE COUNT         1424 a          621 c          765 g         1421 t
ORIGIN      
        1 ttactgtcaa aaaaaaaaaa aaaaaaaata acatttccaa atttgtgtag aaaaaaataa
       61 gaaaacacct aaaattcatg agtgaaaatg cgtacgcatc ttattgtcaa tcttctatta
      121 ttttacattt agttacatta cattgacaaa aatacacaag ctcttacaaa aactattatt
      181 tttgcaaaaa aaatgctatg gcacaaaaac cttctatacc caaaggcacg cgcgatttta
      241 tccctgccga aatgagtaaa cgtaagtata ttttcgatac tattcaatct atttttactt
      301 tgtatggcta tttgcctata gaaactcctg ctatggaaac catgcaaacg cttatgggta
      361 agtatggcga agagggtgat aagttgatat ttaaaatact caattccggt gaatttaatc
      421 aaggaatttc tgccgatgaa tatacacaag caagttcaac tgctttggct cacaaaatct
      481 gcgaaaaggc tttgcgatac gatttaacag tgccttttgc tcggtttgtt gtaatgcatc
      541 aaaacgaaat acaattcccg tttaaacgct atcagataca accagtatgg cgtgccgata
      601 gaccgcaaaa agggagatat cgagaatttt atcagtgtga tgccgatata gttggcagca
      661 attcgttggt aaacgaggta gagttggtgc aaatggttga tgcggtgttt tcaaaactcg
      721 gtattcgtgt gtgcatcaaa ataaacaata gaaaaatatt agccggaata gccgaagtta
      781 ttggcgaatc ggataaaata atagatataa ccgtagctat agacaaactc gaaaaagtag
      841 gtattgaaaa tgtgaataaa gaattgagcg acaaaggtat tgcggattct accatagcga
      901 aattacaacc aattttggaa ttgcacggaa ctaccgaaca aaaattgcaa caattacaaa
      961 caattttaca atcgtcggaa gttggtaaac aaggaattgc cgaaatagaa tatatatgca
     1021 ccaatatttc gcacatgtct ttgtctgccg aatttgagtt ggatttaacg cttgctcgag
     1081 gtcttaatta ttataccgga gctatttttg aagttaagtc aaaagatgtg caaatagtta
     1141 gtatatgcgg aggtgggcga tacgataatt taaccggaat atttggtttg ccgggtgttt
     1201 cgggtgttgg aatttcgttt ggtgccgata gaatttacga tgtattgcaa cagcttaatg
     1261 cctatcctgc gcaggtaaac caaacggcaa aaattctatt tgtgtgtttt ggcgaacctg
     1321 aacaacaaca agcgttactc tatgctcaat caatcagaag caaaggaata gcgtgcgaag
     1381 tttatcccga tgtagctaaa atgaaaaaac aaatgcaata tgccaacgat agaggtattc
     1441 cgtatgttgg aatcattggc gaaacagaac ttgagtcgca tacaataatg cttaaaaata
     1501 tgataacagg cacacaggag gctgtgaata ttgagcaatg tatagctttt ttacaaaaat
     1561 aaatactata atcctttgcc taacagaacg gttcttattg tataaatcaa tattttaata
     1621 tcgagtaata atgacatatt ttctatatat attatgtcat atttcaggcg ttcaatcatt
     1681 tcgtcaacat tttttgcata gccgaatttt acttgtcccc acgatgttat accgggtctt
     1741 acacgttgta ggaaaataaa ttgtggtgca ataggtaata tttgttcaat aaaatattta
     1801 cgttccggac gaggtccaac taacgacatt tcaccaataa gaacattata aaattgagga
     1861 atttcatcga gtcgtgtttt tctcaaaaat ctaccaatag gtgtaatgcg agaatcattt
     1921 tctttagata atgctatgcc ttgtttttca gctcctacat acatagttct aaatttaaaa
     1981 attctaaaag gtctgccata ttttccaatg cgtatttgac gatagaaaac aggacccggt
     2041 gatgacagtt taacacctat agccaaagca atgtaaaggg gtaaaaagag aataagtaca
     2101 ccaatagata ctataacatc tatcatgcgt ttcgtgtttt cttgccaagc cggcatagga
     2161 tttcgagata ttaccattag tggttcatcg tggagtgatg tcatttgtac acgtcccgaa
     2221 atgatatcgc aattatcggg tatagctttt atggtagctt gtatgccttc aagtttatat
     2281 agtatatgtt ctatatattg atgttctttt gattctatgg caattataac ttcttcaaca
     2341 tggtgttttt ttaatacttg ttttacattg tcaatatgac cgagatgagg catatattgt
     2401 ttcattacat aattttcttg ttgaattaca tttacaaatc ctataaaagc attacccgat
     2461 gaacgttttt ttgcttccat gcgttcgtat aattgttttg ccttttcatt actacccacc
     2521 aataaagttg gaaatactaa tatgcgttta tgtattttgt aattggtaat agatgtaatt
     2581 atagctcttg gtatatacgt agcaaaaaaa tgaagtgaaa aaagaagtaa taatgattta
     2641 taatatgaat aatatgagta aatattgtca tcaagtaata aagtaaaaaa tataactaat
     2701 gtgccaatga gagtaagtat aaaagtttga ataagttcta ttaagcgtga ttttctgaaa
     2761 atgtctttat aatacccgtt tatgtaatag agtaaaaccc acataattgg tatgattata
     2821 agaccataaa tgtaattttt gtcgaaagaa atttcgtttg aaaatttcat aaaatataca
     2881 tcaatatatt tcttccgcat caaaaagaat aatgtccatg caattgcagc tgctaaaaaa
     2941 tcagaaatta tatagagaag agtttgtctt gttttgttca ttgttacccc attgtatata
     3001 gttacaaata taatgtattt tgcatacatc aaatagagtt tgcaaaaaaa agacatgtaa
     3061 actatgggtt accctataat aggttctgag tacattatgg agtactgcaa ttattacaca
     3121 ttttaatact tgaacgtttt gtgtgaactg tttttttgaa tctatgtgtt tgctcagatg
     3181 taattatttc aagaaaagat ttgtctgtta gtgaaccata ggcaaacgta tgatttttgt
     3241 cgaaacaaca aggtaatatg gttccttgcc aacttattac gcatgtgctc cacatacgcc
     3301 agcaggcatt tttaagtggt tgttttatgt gaatagtatt atcttttatt gtatatcggc
     3361 tatactgttt atcggggagt aggtcaatat gttcggcaga gtataattga gcagttttga
     3421 aaactacctt atcggctccg tattttttac ccaattttga aattgtttgt gtatgatgtt
     3481 gagtgtgttt aaacaataaa cattgtaact caataatagg agtgtgagat tgtaatgttt
     3541 gttttgcctg ttgcagagca gttaatgctt gcgttacttt ttcaaatgaa ccttgtttgc
     3601 gatatatttc gtaggtttct tgattgtatc cgtcaaatga aactataaga tgagtaaggc
     3661 ctgaacgcac aattgcttca gcatttttag aatctataaa atgagcgttg gttgaaattg
     3721 atgttataat acgcgcttta tgcaattgag taatcatgtg cacaagttgg gtatgcaaaa
     3781 atggttcgcc ttgaaaatac aaatttaccg caattgtata cggtttacat gcttcaacaa
     3841 tagaagtaaa tgtttggggt gatatatatc ctttgcttac agtacttgtt ttgttacctg
     3901 tggggcattc cggacattgt aaattacaaa tatttgttgg ttctatgctt acaaatgccg
     3961 gaggataagt tgtaacatgt ttttttaaaa atatggatat gtaaaaagta aaacgaagca
     4021 tacagtaatt ccatattttt ttgaaagtta atgatttaaa aatataatag ataaaaagca
     4081 gataatactt cattacaggt atatttgtgt gtggagataa ataaattatt gttgtttatg
     4141 ccaccaatgt atatttttat tatatctaga actatatgat ttatgaattg aatggatact
     4201 ccaagttaga ccattatatt cggttttcat t
//
LOCUS       DAOTOW010000097         4231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_16975_length_4231_cov_3.221743, whole genome shotgun sequence.
ACCESSION   DAOTOW010000097 DAOTOW010000000
VERSION     DAOTOW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16975_length_4231_cov_3.221743"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="P5243_02485"
     CDS             complement(<1..1065)
                     /locus_tag="P5243_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRS18345.1"
                     /translation="MRMSKKFDAFVTWASGIKVPINKKILDQMAAWSSSMNVKVNKSM
                     LLSWRVLETNSMFADRISDVPKSFIREILKVAISPNIISFAGGLPNRDLFPVEALKDA
                     SNRVFEGDAADALQYSTTEGYLPLREYISERYKTAKGLNIEPKNILITTGSQQGLDLL
                     GKTFLNEQDRVIIEKPGYLGAIQLFSIYKARFSPVTLKSDGLDLIELTKASSLRNTKL
                     MYTVPNFQNPTGITYSDENRQAVADILRGKPLMLIEDDPYGDLRYTGSHQSSFMHYLP
                     EQTILLGSFSKTVVPSFRTGWIVAPDEIMEKLVIAKQASDLHTNYFTQKLLVEYLHNN
                     DLNEHINKIKTVYGRQCMAMI"
     gene            complement(1130..2959)
                     /gene="ilvD"
                     /locus_tag="P5243_02490"
     CDS             complement(1130..2959)
                     /gene="ilvD"
                     /locus_tag="P5243_02490"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRS18346.1"
                     /translation="MGFPLRSESSTQGRLMAGARSLWRANGMKEEQIGKPIIAIANSF
                     TQFVPGHVHLHDIGQKVKQLIEAHGFFAAEFNTIAIDDGIAMGHTGMLYSLPSRELIA
                     DSVEFMCNAHKADALVCISNCDKITPGMLIAAMRLNIPTIFISGGPMEAGDVDGEKLD
                     LVDAMVMALDADVSDAKLDAVEKNACPTCGSCSGMFTANSMNCLNEAIGLALPGNGTI
                     VATHVNRTKLFEQAAHKIVENTKKYYYEGDTSVLPRSIATRSAFMNAMKLDIAMGGST
                     NTVLHLLAIAYEAQVPFSMKDMDELSRQIPCLCKVAPNSKKYHIEDVNRAGGILGILG
                     ELDRKGLLDTSVSRVDGLTLKQALDAYDIMRPTADTKATEIYKSAPGYKANLVMGSQQ
                     NMYKEFDTDRTNGCIRSVEHAYTQDGGLAVLFGNIAENGCIVKTAGVDESIFRFEGTA
                     RVFHSQEAAVDAIVKGFIKSGDVVVIIYEGPKGGPGMQEMLYPTSYLKSMHLGKECAL
                     ITDGRFSGGTSGLSVGHVSPEAASGGAIGLVQDGDKILFDIPNRKVELVVSADELTAR
                     RNAEIAKGNKAFKPNRVREVSAALKVYAAHVSSADLGGVRIID"
     gene            3448..3678
                     /gene="thiS"
                     /locus_tag="P5243_02495"
     CDS             3448..3678
                     /gene="thiS"
                     /locus_tag="P5243_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRS18347.1"
                     /translation="MKITVNGEAKQYEQAVVTVADLLVLEQVASPEMVSVQRNGDFVA
                     TEQFETTMLEDGDEIDFLFFMGGGSITDFLEL"
     gene            3707..>4231
                     /locus_tag="P5243_02500"
     CDS             3707..>4231
                     /locus_tag="P5243_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRS18348.1"
                     /translation="MDLTEQQIERYSRHILLQQVGYEGQLKIAQGKVLIIGAGGLGAP
                     AALYLAAAGVGTIGIIDADVVDITNLQRQVIHHTPDIGVAKVTSAANKIAEINPDVRV
                     IQYHSWFSPDNALEIISDYDFIIDGTDNFPTKFLINDACVMAGKAFSHGGILRFEGQT
                     FTHIPGTACYRCIFP"
BASE COUNT         1278 a          813 c          760 g         1380 t
ORIGIN      
        1 tatcatagcc atacattgac gaccatatac tgttttaatt ttgttgatat gttcattcaa
       61 atcgttgttg tgcaagtatt ctaccaataa tttttgtgta aaatagttgg tatgtaaatc
      121 tgaagcttgt tttgctatta ctagtttttc cattatttca tcaggagcca ctatccaacc
      181 agttcgaaac gaaggaacta ctgttttaga aaatgaacca agtaaaattg tttgttcagg
      241 taaatagtgc ataaatgatg actgatgaga gcctgtgtat cgtaaatcac catacgggtc
      301 atcttctata agcataagtg gtttgcctcg taatatgtca gcaacagctt gtctgttttc
      361 gtcgctatat gtaatacctg taggattttg aaaatttggt accgtataca ttagtttggt
      421 atttcgcaga ctcgatgctt tagttaattc aattaaatcc aaaccatcag atttcaatgt
      481 tacaggtgaa aatctcgctt tataaataga gaataattgt atagcgccta aatatccggg
      541 tttttcaatg attactcgat cttgttcgtt taaaaatgtt ttacccagta agtctaaacc
      601 ttgttgtgaa cctgtggtta taagaatatt tttaggctca atatttaacc cttttgccgt
      661 tttgtatcgt tcagaaatat attctctgag tggtaaatac ccttcggttg tactatattg
      721 caaagcatcg gcagcatcgc cttcaaatac tctattcgat gcatctttga gtgcctcaac
      781 cggaaataaa tcacgattag gtaatccacc tgcaaacgaa ataatattag gagaaatggc
      841 aacttttaaa atttcgcgaa taaacgattt gggtacgtca gaaattctgt cggcaaacat
      901 gctgttggtt tcgagcacac gccacgaaag caacatagat ttgtttactt ttacattcat
      961 acttgatgac catgcagcca tttgatcaag tatttttttg tttatcggaa ctttaattcc
     1021 cgatgcccat gttacaaaag catcgaattt ttttgacatc ctcatatgtg tattttttta
     1081 aagtctaaaa ggtcttaaaa gtctgaacag tcttattagt ctagttattt tagtctatta
     1141 ttcgtacacc acctaaatct gctgacgata catgtgctgc atatactttg agtgctgcac
     1201 ttacttcgcg tacacggttt ggtttaaatg ctttgttacc ttttgcaatt tctgcatttc
     1261 tgcgagctgt caattcatct gcacttacca caagttcaac cttacgattt ggtatatcaa
     1321 acaatatttt atctccatcc tgaactaaac ctattgcacc accacttgct gcttcgggcg
     1381 atacgtgacc aaccgataaa cccgatgtgc cgccagagaa tctaccatcg gtaattaatg
     1441 cacattcttt tccaagatgc atcgatttaa ggtatgaagt aggatacaac atttcttgca
     1501 tgcccgggcc tcctttaggc ccttcgtata taattactac cacatcgccc gatttgataa
     1561 atccttttac aattgcatct acggcagctt cttgcgaatg gaatacacga gcggttcctt
     1621 caaaacggaa aatagattca tcaacaccgg ctgtttttac aatacaacca ttttcggcaa
     1681 tatttccaaa caatactgcc aatccaccat cttgtgtata tgcatgttct acactacgaa
     1741 tacaaccgtt ggtgcggtcg gtatcaaatt ctttatacat attttgttga gaacccataa
     1801 ccaaatttgc tttgtagcca ggtgcacttt tgtatatctc tgtagctttg gtatcggcag
     1861 ttggtcgcat gatgtcgtaa gcgtcaagtg cttgtttgag tgttaaacca tcaacacgcg
     1921 aaacggaagt gtcaagtaat cctttacggt caagttcgcc aagaattcca agaattccac
     1981 ctgcacggtt tacgtcttct atatggtatt ttttagaatt aggagctact ttgcataaac
     2041 aaggaatttg acgtgaaagt tcatccatat ctttcatgct aaacggcact tgtgcttcgt
     2101 acgcaattgc caacaagtgt aatacggtat tggtagagcc tcccattgca atatcaagtt
     2161 tcatagcatt cataaatgca ctgcgtgtag ctatagaacg aggcagtaca gaagtgtcgc
     2221 cttcgtagta atatttcttt gtgttttcta caattttgtg tgcagcttgt tcaaatagtt
     2281 tggtacgatt tacatgggtt gctacaatag ttccattacc gggtaatgct aaacctattg
     2341 cttcattaag acaattcata gaatttgccg taaacattcc cgaacatgac ccacaggtag
     2401 gacatgcatt tttttcaact gcatctaatt ttgcatcaga tacatcggca tcaagagcca
     2461 taaccattgc gtcaaccaag tctaattttt ctccatctac atctcctgct tccatagggc
     2521 cacccgaaat aaaaatggta ggaatattca aacgcatagc agctataagc attcccggtg
     2581 tgattttgtc gcaattgctt atacaaacta acgcatcggc tttgtgtgca ttacacataa
     2641 attctacgct gtcggcaatt aattcacgcg atggcaatga atataacatg ccggtatgtc
     2701 ccatagctat tccgtcgtct atggcaattg tattgaattc tgcagcaaaa aaaccgtgcg
     2761 cttctattag ttgcttaact ttttgtccaa tatcatgtaa atgtacgtgc ccaggaacaa
     2821 attgtgtaaa tgagttagca atagcaatga ttggtttgcc tatttgctct tctttcatac
     2881 cgttggcacg ccataagctt cgtgctcctg ccataagtcg accttgtgtc gatgactcgc
     2941 ttctaagtgg aaatcccata ttgttataat tttgagtttt gagttttgag ttttgagttc
     3001 aaaatcaaaa tgttaatact attttttttg agttgacaaa tgtacatttt tttacaaaaa
     3061 aaaagcacca aactcataag agaatggtgc tttacatatt ttttacatat acttacgtag
     3121 ttacaccttt ctctgtattg aagaaatgac aaccacgaca agtactatgt taataattct
     3181 attcattttt ttaatttcta ttaagcaaat atactgtata ttttttcaaa gtcaagattt
     3241 tggaaaaaaa atctgtaaaa aaatacatgt gctttgttta cgtatacata ccaaacaacc
     3301 gcatttgcta agcaaaacga taaataaagg tggtaatttt ttagtgtaat tttcaaaaaa
     3361 gaattaaaaa aatttgcttt cgaaaatttt gcattatata tttgtcctac taaaacgata
     3421 ggatttatag tataaaaaga aaatacaatg aaaataacag ttaacggcga agccaaacaa
     3481 tatgaacaag cagtggtaac agttgccgat ttgcttgtat tagaacaagt tgcaagtccc
     3541 gaaatggtgt cggtacaacg aaatggtgat tttgtggcaa cagaacagtt tgaaactact
     3601 atgcttgaag atggtgatga aatagatttt ctcttcttta tgggaggcgg aagtatcaca
     3661 gactttttag aactatagac ttttcagacc ttttagacaa aaaatcatgg acttaacaga
     3721 acaacaaata gaacgatata gccgacacat tctcttgcag caagtaggat acgaaggcca
     3781 actgaaaata gcgcaaggta aagtacttat aataggtgcc ggcggcttgg gtgctccggc
     3841 agctttatac ctggctgcag ccggtgttgg aactattggg attatagatg ccgatgtggt
     3901 tgatattacc aatttgcaac gccaagtaat acatcatacg cccgacatag gagtagctaa
     3961 agtaacatcg gctgccaaca aaattgcgga aatcaatccc gatgttcgtg ttattcaata
     4021 ccacagttgg tttagccccg acaatgcgtt agaaattatt tccgactatg attttattat
     4081 agatggtacc gataattttc caacaaaatt tttgataaac gatgcctgtg taatggcagg
     4141 taaagcattt tcgcatggtg gaatactgcg attcgaagga cagacattta ctcacattcc
     4201 gggcacggca tgttaccgat gtatattccc c
//
LOCUS       DAOTOW010000098         4196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17146_length_4196_cov_3.149722, whole genome shotgun sequence.
ACCESSION   DAOTOW010000098 DAOTOW010000000
VERSION     DAOTOW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17146_length_4196_cov_3.149722"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="P5243_02505"
     CDS             <1..615
                     /locus_tag="P5243_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18349.1"
                     /translation="ACKHKSKLSAFHQTIHLGVSPGTDGVSNVLDWTDYIGESGEFVP
                     VWYYIYRGTTKNNLVLHDSVDAKVASEWNDTKPMGSKYYKIGVKKEQACVTSGILKIE
                     SGPYTLAMSNIAEAQTGETSILTPQLYTQVYPNPSTGDITIDIQSQYKCKATLSNMAG
                     IVVWKQDQINPGETVYIHSLPQGLYTLWVQCGNTKHSQIIVIQK"
     gene            757..1887
                     /gene="tgt"
                     /locus_tag="P5243_02510"
     CDS             757..1887
                     /gene="tgt"
                     /locus_tag="P5243_02510"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRS18350.1"
                     /translation="MQFTISSTDTHSQARAGTISTDHGTIQTPIFMPVGTVGSVKGIH
                     MRELRDDINAQIILGNTYHLYLRPGLDTIRKAGGLHTFNGWNKPILTDSGGYQVFSLS
                     HNRKLTEEGAVFKSHIDGSKHIFTPENVVDTQRIIGADIIMALDECTPYPCEYNYAKK
                     SMELTHRWLARGIEHFTKTEPLYGYTQTFFPIVQGSVYKDLRAQSAEYIASHNMEGNA
                     IGGLSVGEPVEDMYEMLTIVNSILPKDKPRYLMGVGTPVNILEAIALGTDMFDCVMPT
                     RNGRNGMLFTWNGIINIKNKKWQNDFSPIDEHSSCFVDLHYTKAYLRHLIYAGEMLGA
                     QIASIHNLAFYIDLVTQARNQIINGTFTEWKKIMISRLSQRL"
     gene            1892..2971
                     /locus_tag="P5243_02515"
     CDS             1892..2971
                     /locus_tag="P5243_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRS18351.1"
                     /translation="MVKKIDLYIIKRFLGTFLFAILIIMTIAIVFDIQEKYEEFIKNS
                     APLKEIIVDYYLNFIPYYVNLFSAMFVFISVIFFTSKMAQNAEIIAIHASGISFNRFL
                     RPYMISATLIAILSWFLIMYIIPESNKVKLAFEDKYVFTRFQNWERNIHRQVRPGIFI
                     YMESYNVDAENAFRFSMEKYENGKMVSKLTSDYARWDSEKKKWIVYNYVIRDIEKGKQ
                     KITTGQILDTVFYLLPEDFKMRTMYVEKMNIDELNEYIEIQRLQGTDNINRLLVQKYQ
                     RWAYPFSTFILTILGVSLSNRKKRGGTVINIVIGLALSFSYIMLMQVTTTFTINSDLD
                     PRIAVWIPNIVYSIICLFLYSKARI"
     gene            2971..3633
                     /gene="mnmD"
                     /locus_tag="P5243_02520"
     CDS             2971..3633
                     /gene="mnmD"
                     /locus_tag="P5243_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HRS18352.1"
                     /translation="MQRFVVKTSDGSNTLYIPQLQEHYHSTYGAHNESQHIYITNAYN
                     YSTKQTPIILEIGFGTGLNAMNTLVEAIKQKKHTTYICIEKYPLNIDEVNNLQYEQLW
                     EENKQSYFTDIHTCAWGVPIYIHQYFTLLKLNIDIVTIHKLPACNIVYYDAFAPEKQP
                     ELWTEEIFARIYACMESQGILTTYCVKGTVRRCLEAIGFTIKKIPGPPNGKREMLRAI
                     KP"
     gene            3748..>4196
                     /locus_tag="P5243_02525"
     CDS             3748..>4196
                     /locus_tag="P5243_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRS18353.1"
                     /translation="MEQKTHIQNGNWDAVFETIYSQYYTGLCTYAHSFIPDADEAEEI
                     VQGIILKMWEQKENLENIISLKSYLFRSVKNTCLNYIKHQKLEEKYREKAWVELKKIE
                     ADFIDPYHNTELEHHISEAIKELPERCREVFEMSRFEGKKNKEISDY"
BASE COUNT         1585 a          665 c          687 g         1259 t
ORIGIN      
        1 gcgtgtaaac acaaatctaa attaagtgca tttcatcaaa caattcattt aggtgtttca
       61 cccggaaccg atggtgtaag taatgtgtta gactggactg attatattgg cgaatcagga
      121 gaatttgttc cggtttggta ctatatttat cgtggaacta caaaaaataa tttagtctta
      181 cacgattcgg ttgatgcaaa agtagctagc gaatggaatg atacaaaacc aatgggttca
      241 aaatattaca aaataggtgt taaaaaagaa caagcttgtg ttacaagtgg aatattaaaa
      301 attgaaagtg gcccctatac tttagctatg agcaacatag ccgaagctca aaccggtgaa
      361 acatcaattt tgacaccgca actttacaca caagtgtacc caaacccatc taccggtgat
      421 ataacaattg atatacaatc acaatacaag tgcaaagcta ctttgagcaa tatggccgga
      481 attgtagttt ggaaacaaga ccaaatcaat ccaggcgaaa ctgtatatat acattcactg
      541 cctcaaggtt tatacacgtt gtgggttcaa tgtggtaaca ctaaacattc tcaaattatt
      601 gttattcaaa aataacagta attccatata gaaaacggtt tcacaagaat ttatttgtga
      661 attatacttg tattgatttt atttaaaata acgcgatttt tagcagacta ctcgtgtata
      721 taactgcttt atattatatt tgccaaaatt ttttacatgc aatttacaat atcatctact
      781 gatactcatt cacaagcacg tgccggaact atcagtaccg accacggaac aatacaaaca
      841 cctattttta tgcctgtagg tacggttggt tctgtaaaag ggatacacat gcgtgagtta
      901 cgtgatgata taaatgctca gataatttta ggaaacacat accatttata tctccgtccc
      961 ggacttgaca ctattcgtaa agccggtggt ttacatacat tcaatgggtg gaataaacca
     1021 attttaaccg atagcggcgg ataccaagtt ttctcccttt cacacaatcg aaaacttacc
     1081 gaagaaggag cagtatttaa atcgcatatt gacggttcaa aacacatatt tactcccgaa
     1141 aatgtagttg acacacaacg aattattgga gcagatatta taatggctct tgatgaatgc
     1201 acaccatacc catgtgaata caattatgca aaaaaatcaa tggaattaac tcaccgttgg
     1261 cttgctcgtg gtatagaaca ttttacaaag acagaaccat tgtatggata cacacaaacg
     1321 tttttcccta tagtacaagg aagcgtatat aaagatttgc gggcacaatc ggcagaatat
     1381 attgcatcgc acaatatgga aggcaatgct ataggcggat taagtgttgg cgagcccgta
     1441 gaagatatgt atgagatgct tactattgta aacagcattt tgccaaaaga caaaccaaga
     1501 tatttaatgg gagttggaac gcctgtaaat atattagaag ccatagcttt gggtaccgat
     1561 atgtttgact gtgtaatgcc aactcgcaat ggaagaaacg gaatgctatt tacatggaat
     1621 ggtataatta atataaaaaa caaaaaatgg caaaacgatt ttagtcctat tgatgaacat
     1681 agttcatgtt ttgttgattt gcattataca aaagcgtatc ttcgtcactt aatttatgca
     1741 ggcgaaatgt taggtgctca aatagcaagt attcataatt tagcatttta tatagatttg
     1801 gttactcaag cacgaaatca aataataaac ggaacattca cagaatggaa aaaaataatg
     1861 atttcacgac ttagtcaacg attataagac aatggtaaaa aaaatagatc tatacataat
     1921 taaacgattt ttgggaacat tcttgtttgc catacttata attatgacaa ttgccatagt
     1981 gtttgatata caagaaaaat atgaagaatt tataaaaaat tcggcacctc tcaaagaaat
     2041 cattgttgac tactatctca attttatacc atattatgta aacttgttta gtgcaatgtt
     2101 tgtatttatt tcggtaatat tttttacgtc aaaaatggct caaaatgcag aaattattgc
     2161 aatccatgcc agtggcatca gtttcaatcg ttttttacgt ccctatatga tttctgctac
     2221 attaatagct atattatcgt ggtttcttat aatgtatata atacccgaat caaacaaagt
     2281 aaaattagca tttgaagata aatatgtttt tacccgattc caaaattggg aacgaaatat
     2341 tcacagacaa gtacgtcccg gtatatttat atatatggaa agctataatg tagatgcaga
     2401 aaatgcattt cgcttttcaa tggaaaaata cgaaaacgga aaaatggtat caaaactcac
     2461 cagcgactat gctcgttggg attcagaaaa aaagaaatgg attgtgtata attatgtaat
     2521 tcgtgatata gaaaaaggaa aacaaaaaat taccacagga caaatacttg atacggtttt
     2581 ttacttatta cccgaagatt ttaaaatgcg tactatgtat gttgaaaaaa tgaatattga
     2641 cgaactcaat gaatatattg aaatacaacg tttacaaggt accgacaata ttaatagatt
     2701 attggtacaa aaatatcaac gctgggcata cccgttttca acttttattc ttacaatttt
     2761 aggagttagt ttatctaata gaaaaaaacg aggaggtaca gtcataaata tagtaatagg
     2821 tttagctctt tcattttcat atataatgct aatgcaagta actactactt ttaccataaa
     2881 ttccgattta gatcctcgca tagcggtatg gattcctaac atagtatata gtataatttg
     2941 tttattttta tatagtaaag cacgaatttg atgcaacgtt ttgtagtaaa aacttccgac
     3001 ggaagcaaca cgctatatat accccaacta caagaacatt atcattctac gtatggagca
     3061 cacaacgaat cacaacatat ttacataact aatgcatata attattctac gaaacaaaca
     3121 cctattattt tagaaattgg gtttggtaca ggactcaatg ctatgaatac tttagtagag
     3181 gcaataaaac aaaaaaaaca tacaacatac atatgtattg aaaaatatcc tttaaacatt
     3241 gatgaagtaa acaatttaca atacgaacaa ttgtgggaag aaaacaaaca atcatatttt
     3301 acagatattc atacatgtgc ttggggtgta ccaatataca tacatcaata ttttactcta
     3361 ctaaaactaa atattgatat agttacaata cacaaacttc ctgcttgtaa tattgtatat
     3421 tacgatgctt ttgcccccga aaaacaacct gaactttgga cagaagaaat atttgctcgt
     3481 atatatgcat gtatggaatc acaaggaata cttactacat actgtgtaaa aggaactgta
     3541 cggcgatgct tagaagcaat tggatttaca attaaaaaaa taccaggccc cccaaatggt
     3601 aaaagagaaa tgttgcgagc aattaaacca taaatagtaa aaaagtatac atttacaatg
     3661 taataaaaaa taagaatttg aaacgataaa aaaaaattgt atatttccac aatttttttg
     3721 tgaaacttat ttttacttca aaacaaattg gaacaaaaaa cacacataca gaatgggaat
     3781 tgggacgcag tgtttgaaac aatttattct caatattata ccggtttgtg cacttatgcc
     3841 cattcattta ttcccgatgc tgacgaagct gaagaaatag ttcagggtat aattttaaaa
     3901 atgtgggagc aaaaagaaaa tcttgaaaac attatttcac tcaaatcata tttattcaga
     3961 tcggtaaaaa atacgtgttt aaactatata aaacaccaaa aattagaaga aaaatataga
     4021 gaaaaagcat gggtagaact caaaaaaata gaagctgatt ttatagaccc gtatcataac
     4081 acagaacttg aacatcatat aagcgaagct attaaagaac tgcccgaacg ttgccgtgaa
     4141 gtatttgaaa tgagtagatt tgaaggtaaa aaaaataaag agatttcaga ctattt
//
LOCUS       DAOTOW010000099         4172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17256_length_4172_cov_3.526597, whole genome shotgun sequence.
ACCESSION   DAOTOW010000099 DAOTOW010000000
VERSION     DAOTOW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17256_length_4172_cov_3.526597"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5243_02530"
     CDS             complement(<1..228)
                     /locus_tag="P5243_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18354.1"
                     /translation="MKKIIIIISILCLSEIGFAQNNNIFIRKEDNPELKVTIDAYFGI
                     SLMTQYNGFIRYGYIQIKSTGETKITYLSQQQ"
     gene            complement(325..1290)
                     /gene="bioB"
                     /locus_tag="P5243_02535"
     CDS             complement(325..1290)
                     /gene="bioB"
                     /locus_tag="P5243_02535"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRS18355.1"
                     /translation="MIDLQKLTEEVCAGKALTYDEGVELTQYPDKEALYEAADTIRTH
                     FCGNNMDLCSITNAKSGRCSQNCKWCSQSAHHTTNIEEYDLIDKERAVNEALESAKAG
                     VHRHSLVTSGKSVSNKTLHSLLEIYKEIREKSSINLCASMGLVTKEQLQKLKNAGISH
                     YHCNLETAPSYFPNVCTTHTIQDKIDVILTAQSLDMRVCSGGIIGMGETMEQRVELAC
                     TLRDLHIMSIPINILTPIEGTPLYNAHTLSEEEILTTIAVFRFINPKANLRFAGGRLQ
                     IKKFQHKALHAGINSALTGNYLTTVGSNTAQDIKDFTASGFTIAR"
     gene            complement(1369..2448)
                     /locus_tag="P5243_02540"
     CDS             complement(1369..2448)
                     /locus_tag="P5243_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRS18356.1"
                     /translation="MEVQLFKHSQLLAFTQEVLQAIGHSAEDAALAADVLVSADLRGV
                     DSHGVARLRGYINLWKAQRMNPKPVITIEHETPSTATVNGDLGLGLVVAPKAMEIAIE
                     KAKQVGTGWVAINNSNHYGIAGYHAMMALKHDMIGISLTNATPTTAPTFGKQRMLGTN
                     PISVAFPTKNQPPFVADFSTTTAAVGKLEILQRKNMDTPTGWIQDAEGNIITDAHGLK
                     KGGSLLPLGSDREHSSHKGFCLGSIVDILCAVLSGANYGPWVPPFLAFMQPSAQQVGK
                     GIGHFVGAMRIDAFRPADDFKKHMDNWIETFRKAETTGENDRIIIPGDPEREMTQERL
                     ISGIPLLMPVVDDLLDIGKTFGVTL"
     gene            complement(2429..3748)
                     /locus_tag="P5243_02545"
     CDS             complement(2429..3748)
                     /locus_tag="P5243_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18357.1"
                     /translation="MNVILPLCFIAIFIVIIYKHGFFKNSGLSFQILIGLFLLKIVAA
                     CALHFLYSFYYTERLEADIFKYFDDALILFKSCKDSPVLFFKLFFSIDIHNPALTPYY
                     DALNFWERKIDYGIFNDNRTIIRINALLMFVSWGNIFVHHIIASFVSLIAFLLIFKVF
                     TYYFQQHTTLFIISIFLIPSSLFWASALLKEMVVMLGLGLLLYGIHSYTITSYRKYII
                     IIGLGILVMSTIKIYVIVACIPAILSFIISHTFQHMSSWKIYISIYTLSIVSVIINHY
                     VHIVPFLETIASKRNDFIMDSAYYAHARSYIEIGMLKSTLSAFIYETPKALLRAFQLP
                     TIIHIQSPIEILPAIENFVGLLLVICMIFWFKKPNKKQQNIIWFSVFFSIGLLWFVGI
                     STPVVGAIVRYKMPMLPFLYTSLALCIDWKACKNVFVRKDIYGSSII"
     gene            3791..4153
                     /locus_tag="P5243_02550"
     CDS             3791..4153
                     /locus_tag="P5243_02550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS18358.1"
                     /translation="MTIPYKNSDKSKKEQVAGMFNNIAHKYDFLNHVLSLGIDTIWRN
                     RAIREMKKNQPKIILDVATGTGDFAIAAARKLTPDLIIGIDISHNMLEIGKQKVSKKQ
                     LDDCIQMQYGDCESSSAR"
BASE COUNT         1443 a          659 c          666 g         1404 t
ORIGIN      
        1 ttgttgttgt gagagatagg taatttttgt ttcacctgta gattttattt gaatatatcc
       61 atatcgaata aaaccattat attgagtcat taaacttatt ccaaaatacg catcaatggt
      121 tactttaagt tcaggattat cttctttacg aataaaaata ttattgtttt gagcaaatcc
      181 gatttccgaa aggcacaaaa tcgaaataat tataattatt tttttcatag cactaagatt
      241 tttacgaaaa tacaattttt tttagctaaa aaaaattctt ccaaaaatct tgcctaaaaa
      301 gagttttata tattattaaa aaaattatcg agctatagta aaaccacttg ccgtaaagtc
      361 tttaatatct tgcgcagtat tagaaccaac tgttgttaaa taattacctg taagagctga
      421 atttataccg gcatgtaaag ctttgtgttg aaattttttg atttgtaatc tgccgccggc
      481 aaaacgtaaa tttgccttag gattaataaa tctaaaaacg gcaatggttg ttaaaatttc
      541 ttcttctgat aatgtatgag cattatataa tggagtacct tctataggag ttaatatatt
      601 tataggaata gacataatat gtaaatcgcg aagtgtacaa gcaagttcta cacgttgctc
      661 catagtttcg cccataccta taatacctcc cgaacataca cgcatgtcaa gagattgagc
      721 agtaagaatt acatcaattt tatcttgaat agtatgtgta gtacaaacat ttggaaaata
      781 tgacggagca gtttctaaat tacagtgata gtgagaaatt ccggcatttt ttaatttttg
      841 taattgttct ttggttacta aacccattga agcacataaa ttaatcgaag atttttcacg
      901 aatttcttta taaatttcga gtaaagaatg taatgtttta ttacttactg attttccgct
      961 ggtaacaagt gaatgtctat gaacacctgc tttagcactt tctaaggctt cgtttactgc
     1021 tcgttcttta tcaattaagt catattcttc tatattggta gtatgatgag cagattggct
     1081 acaccattta caattttgtg aacaacgacc ggatttggca tttgttatag aacacaaatc
     1141 catattgtta ccacaaaaat gagttcgtat agtatctgct gcttcatata atgcttcttt
     1201 atcggggtat tgtgttaatt caactccttc atcgtatgta agtgctttac ctgcacatac
     1261 ttcttctgtt aatttttgta aatctatcat tgtattttgt aaattattac atattactga
     1321 ttataaccac taatactaag ttttttaata tatggtaatt gtatggaatt ataatgtaac
     1381 accaaatgtt ttgccaatat ccagtaaatc atcaactacc ggcataagta acggtatgcc
     1441 gcttattaga cgttcttgag tcatttctct ttcagggtca ccgggaatta ttattctatc
     1501 attttctccg gtagtttctg cttttctgaa tgtttcaatc caattgtcca tgtgtttttt
     1561 aaaatcgtct gcgggtctaa atgcatctat tctcatggcg ccaacaaaat gtccaatacc
     1621 ttttcctact tgttgggcac ttggttgcat aaatgctaaa aatggtggaa cccatggtcc
     1681 ataattagca cctgataaaa cagcacaaag tatatctact atagaaccta aacaaaatcc
     1741 tttgtgactg ctgtgttctc tatcagaacc taaaggtaat aaagagccgc cttttttgag
     1801 accatgagca tcggtgatta tattaccttc agcatcttgt atccagccgg ttggagtatc
     1861 catattttta cgttgcagaa tttctaattt accaacagcg gcagtggtag tagaaaaatc
     1921 tgctacaaat ggaggttgat ttttggtagg gaatgccaca gaaatggggt tagtgcctaa
     1981 cattcgttgt ttgccgaatg taggggcagt agtaggagta gcattggtaa gggaaattcc
     2041 aatcatatcg tgttttaaag ccatcatagc atggtaacct gcaattccat agtggtttga
     2101 attgtttatg gcaacccagc ctgttcctac ttgttttgct ttttcaattg caatttccat
     2161 agccttagga gctactacta atcctagacc taaatcaccg tttacagttg cggttgatgg
     2221 agtctcgtgt tctattgtaa ttacaggttt aggattcatt cgttgagctt tccatagatt
     2281 aatataacca cgtaagcgtg ctactccatg tgaatcaact ccacgtaaat cggctgatac
     2341 taatacatcg gcagctaatg cagcatcttc ggcactatga cctattgctt gcaatacttc
     2401 ttgtgtaaat gcaagtagtt gagaatgttt aaataattga acttccatat atatcttttc
     2461 ttacaaatac atttttacaa gccttccaat caatacataa agcaagactt gtgtagagaa
     2521 acggtaacat tggcattttg tatcgtacaa ttgctccaac tacaggtgta cttattccaa
     2581 caaaccacag caaacctatt gaaaaaaata ctgaaaacca aataatattt tgttgtttct
     2641 tattgggttt tttgaaccaa aaaatcatac aaatcactag tagtaatccc acgaaatttt
     2701 caatagcagg aagtatttct atagggcttt gtatatgtat aattgtaggt aattgaaatg
     2761 ctcgtaatag agctttgggt gtttcatata taaatgcact taaggttgat ttaagcattc
     2821 ctatttctat atagctccgt gcatgagcat aatatgcaga atccataata aaatcattgc
     2881 gtttagatgc aatagtttct aaaaaaggta ctatgtgtac atagtgattt attataacag
     2941 atacaatact taatgtatat atactaatat atattttcca tgatgacatg tgttgaaatg
     3001 tatgacttat aataaaactt aaaattgccg gaatacatgc aactattaca tatattttaa
     3061 ttgtactcat tactaaaatt cctaagccaa ttattattat atattttcta tatgaagtaa
     3121 ttgtgtatga atgaatacca tataaaagta atcctaatcc cagcattaca accatttctt
     3181 tgagtaatgc cgatgcccaa aaaagggatg aaggaattaa aaaaatagaa atgatgaaaa
     3241 gtgttgtatg ttgttgaaaa taataggtaa atactttaaa tatgagcaaa aatgctatta
     3301 atgatacaaa cgaagcaatt atatgatgaa caaaaatatt accccatgaa acaaacataa
     3361 gcaatgcatt gattctaatt attgttctgt tgtcattaaa aatgccgtaa tctattttac
     3421 gttcccaaaa attaagggca tcataatatg gggttaaagc aggattgtga atatcaatag
     3481 aaaaaaatag tttgaaaaat aatacaggtg aatctttaca agatttaaat aaaataagag
     3541 catcgtcaaa atatttaaaa atatcggctt ctaaacgttc ggtataataa aaactataca
     3601 aaaaatgaag tgcacatgca gctacaattt taagcaaaaa aagacctata agaatttgaa
     3661 atgacagtcc cgaattcttg aaaaatccat gcttatagat gattactata aaaattgcaa
     3721 taaaacacaa aggcaaaatc acattcattt gtatatttgc taaaatattt ctcaaaaatc
     3781 acaaaataaa atgactattc catacaaaaa ttcagataaa agtaaaaaag aacaagttgc
     3841 cggtatgttt aataatattg cgcataaata cgattttctg aatcatgtat tatcattagg
     3901 aatagacact atttggcgga atagagctat acgagaaatg aagaaaaatc aaccaaaaat
     3961 aattcttgat gtagcaaccg gaaccggcga ttttgctata gctgcagctc gtaaacttac
     4021 tcctgattta attataggaa tagatatttc acataatatg cttgaaatag gtaagcaaaa
     4081 agttagtaaa aaacagttag atgattgtat acaaatgcaa tatggtgatt gtgaatcatc
     4141 atccgcaagg tagtggtggc acaaacatct ca
//
LOCUS       DAOTOW010000100         4161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17314_length_4161_cov_3.193863, whole genome shotgun sequence.
ACCESSION   DAOTOW010000100 DAOTOW010000000
VERSION     DAOTOW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17314_length_4161_cov_3.193863"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="P5243_02555"
     CDS             <1..462
                     /locus_tag="P5243_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRS18359.1"
                     /translation="LPGSKQEYGGVITHGAKLLFAYGEATVPKVTVTLRKSYGGAHIV
                     MSSKQLRGDINYAWPTAEIAVMGAEGAVEVLYAKEISALETPEAQAAKAEEKKEEYNN
                     LFATPYQAAKYGFIDDVIEPRNTRFRVIRALQRLQTKKDTNPPRKHCNIPL"
     gene            474..842
                     /locus_tag="P5243_02560"
     CDS             474..842
                     /locus_tag="P5243_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRS18360.1"
                     /translation="MNILEIPDLSEGYMMMLVGMSVVFTVLLLLFVAFMQLKQVPNII
                     DKFEQRKNRKEQEKNTQTSKSSSNTELTADVNAAISMALYLYMNEQHDHESEVVTIRT
                     MQRRYSPWSSKIYGLNILKK"
     gene            847..1272
                     /locus_tag="P5243_02565"
     CDS             847..1272
                     /locus_tag="P5243_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRS18361.1"
                     /translation="MKNFKFTINGQTYDVEIKQVEDNNATIEVNGSEYAVEIHQEVKA
                     AAPKTPKLVTKPANLQDGESFMKKVSAGSGIKSPLPGVIMEIHVKVGDNVKRGQKVLT
                     MEAMKMHNDVLAEKDGTVKNIKVSAGQSVMEGDVLVELE"
     gene            1446..2597
                     /locus_tag="P5243_02570"
     CDS             1446..2597
                     /locus_tag="P5243_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRS18362.1"
                     /translation="MHEALSFKKGIERFYKFSSFANFQWGNLIMILVGGFFIFLAIRY
                     DYEPLLLVPIGFGIIIGNVPFLTGLRIGIYEPGSVMNYLYFGVLQGVYPPLIFLGIGA
                     MTDFSTLISNPKLMLLGAAAQFGIFAAFLGALALGFTDDQAGAIGIIGGADGPTAIFL
                     SSKLAPEFMGAIAIAAYSYMALVPVIQPPIMRLLTTDKERVIRMRPPRQVTKLEKILF
                     PIIGLLLTTFITPAAMPLLGMLFFGNLLKESTVTKRLANTAANPLIDIVTILIGVTVG
                     ASTQANVFLTSKSLLIFALGALAFIFATFGGVAFAKFMNLFLKEGNKINPLIGNAGVS
                     AVPDSARVSQHEGLKYDPTNHLLMHAMAPNVAGVIGSAVAAGVLLSFLG"
     gene            2743..3516
                     /locus_tag="P5243_02575"
     CDS             2743..3516
                     /locus_tag="P5243_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRS18363.1"
                     /translation="MRNFLIICITIVIFASCSNADKYRKLETSLDSTSYSLGLLFASK
                     IPQNLENNNIDSISFQHFIQGIRDYFDSTRSTVVTKEESQKIIQHYLEQIMKSSNSQY
                     AAKFISYETEGNIFLDKNKKNPGVTELQKGLQYQIYYAGWGQLQPDIRDTILIHFKIL
                     NIQQKVLYDTRSKSANPIKMPLDSAITAFKRVLPLVYTGAKLRIFSSHEFAYGTSVTS
                     KDIIEPYQTLLFDVELVKTLKGNYVDSVRDSLYKDTVKK"
     gene            3541..>4161
                     /locus_tag="P5243_02580"
     CDS             3541..>4161
                     /locus_tag="P5243_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRS18364.1"
                     /translation="MKSLVKFTTITTVAISLLACNGLKKDKDAKLTNLRDSASYCLGL
                     SVATNLAQDKLDSLNLDAFIAGYLDAKDPESYKIAVAEISNTIQNFAIQEIKIKYSAN
                     IEAGKKFLEENKKRAGVTTTASGLQYEVISMGTGAKPQIYDSVTVNYHGTKLDGSVFD
                     SSKGANPVTLSLTPGKLIQGWVEGMTLFPVGSKFKLYIPEDLAYGVN"
BASE COUNT         1517 a          672 c          685 g         1287 t
ORIGIN      
        1 ttaccaggtt caaaacaaga atacggagga gtaataactc acggagcaaa attactattt
       61 gcatatggtg aagcaacagt tccaaaagtt accgttacat tacgtaaatc gtatggcgga
      121 gctcatatag taatgagtag caaacaatta cgcggagata ttaactacgc atggccaact
      181 gcagaaattg cagtaatggg cgccgaagga gctgtagagg tattgtatgc aaaagaaatt
      241 tctgctttag aaacacccga agctcaagct gcaaaagcag aagagaaaaa agaagaatac
      301 aataacttat ttgcaactcc ttaccaagct gctaaatatg gatttattga tgacgtaatt
      361 gagcctcgca atactcgttt tagagtaatt agagcattac aacgtcttca aaccaaaaag
      421 gatacaaatc caccacgtaa acattgtaat ataccacttt aattgtaatc attatgaata
      481 tacttgaaat acccgattta tcggaaggtt atatgatgat gttagttgga atgtcagttg
      541 tttttactgt tctattatta ctctttgttg cctttatgca attaaaacaa gttcctaaca
      601 taattgataa atttgaacaa agaaaaaata gaaaagaaca agaaaaaaac acacaaactt
      661 caaaatcttc atcaaataca gaacttacag ccgatgtaaa tgctgctata agtatggcgt
      721 tatacttata tatgaatgaa caacacgatc atgaaagtga ggttgttacc attcgtacta
      781 tgcaacgcag atactcacca tggagttcta aaatttatgg attaaatatc ttaaaaaagt
      841 aaaaacatga aaaattttaa attcacaata aacggacaaa catacgacgt tgaaataaaa
      901 caagttgaag ataataatgc aactatagaa gtaaacggtt cggagtatgc agttgaaatt
      961 caccaagaag tgaaagcagc agcacctaaa accccaaaac ttgtaactaa acctgcaaat
     1021 ttgcaagatg gtgaaagttt tatgaaaaaa gtgagtgccg gttctggaat caaatctcca
     1081 ctccccggtg taattatgga aatacatgta aaagtaggtg acaatgtaaa acgtggtcaa
     1141 aaagtactta caatggaagc catgaaaatg cataatgatg ttcttgccga aaaagatggt
     1201 actgtaaaaa atattaaagt atcagcaggt caatcggtaa tggaaggtga tgtacttgta
     1261 gaacttgaat aattaaaaaa caaaagctaa tgaaaaaaat aatatcaaca tttacagtat
     1321 tacttattgt cgttgttggt gttttgttta ccacatcaag cactgttccg caagattcta
     1381 caaacacaac acaacaatca acagaagtaa catctgtaac aaacacgaca aatgacacgc
     1441 aagaaattca tgaagcattg tcatttaaaa aagggataga gcgattctat aaattttcta
     1501 gtttcgcaaa ttttcaatgg ggtaatctta ttatgatatt agtaggagga ttttttattt
     1561 tccttgctat tcgttatgat tacgagccac ttctgctagt accaattggt tttggtatta
     1621 ttataggtaa tgtaccgttt ttaacaggac tacgtattgg tatttatgaa cccggaagcg
     1681 ttatgaacta cttatatttc ggggtattgc aaggggtata tccaccactt atattccttg
     1741 gaattggtgc tatgacagac ttctccacat taatatcaaa ccctaaatta atgttattag
     1801 gtgccgcagc tcaatttggt atttttgctg catttttagg agctcttgct ttaggattta
     1861 ccgatgacca agccggtgct attggaatta ttggaggtgc tgacggccct accgctattt
     1921 tcttatcatc aaaattggct cctgaattta tgggtgctat agccatagcc gcatactcat
     1981 atatggcatt ggttccggta attcaaccgc ctattatgcg tttattaaca accgataaag
     2041 agcgcgttat ccgtatgcga ccaccaagac aagtaactaa attggaaaaa atattattcc
     2101 ctattatagg cttgttgctt accaccttta ttacccctgc tgctatgcct ctgttaggta
     2161 tgttattctt tggaaattta ttaaaagaat ctaccgtaac taaacgttta gcaaataccg
     2221 cagcaaatcc attgattgat attgtaacaa ttttaattgg agttactgtt ggtgcttcta
     2281 ctcaagccaa tgtattctta acatctaaat cattattaat ctttgcttta ggtgcattag
     2341 catttatttt tgcaacattt ggtggagttg cttttgctaa atttatgaat ttattcttaa
     2401 aagaaggcaa taaaataaat ccattaattg ggaatgccgg tgtatctgca gtacccgatt
     2461 cagctcgtgt atcgcaacac gaagggttaa aatatgaccc tactaatcac ttattgatgc
     2521 acgctatggc acctaacgtt gccggagtaa ttggttcggc agttgctgcg ggtgtattat
     2581 tgagtttctt aggataacaa aaaaaaatat aaaaaagagg ctatttgatt ttttcaaata
     2641 gcctcttttt tttctatttt acacaaaaaa acactatgat ttttaaataa aaaactgtaa
     2701 gtttgcactg atttcaaaag aaataattta attaattttt caatgaggaa ttttcttatc
     2761 atttgtatca caatagtaat atttgcatca tgcagtaatg ctgacaaata tagaaaatta
     2821 gaaacctcat tagattcaac ttcatatagt ttaggacttc tttttgccag caaaattcct
     2881 caaaatctag aaaataataa catagacagt atttcatttc aacattttat tcaaggaatt
     2941 cgcgattatt ttgacagcac tcgttctacc gtagtaacca aagaagaatc acaaaaaatt
     3001 atacaacact atttggaaca aataatgaaa agttctaatt ctcaatatgc tgcaaaattt
     3061 atttcatatg aaacagaagg aaatatattt ttagataaaa acaaaaaaaa tcccggtgtt
     3121 accgaactac aaaaaggatt acaatatcaa atttattatg ccggttgggg acaattacaa
     3181 cctgatattc gtgatacaat tcttattcat ttcaagatat taaatattca acaaaaagta
     3241 ctctatgata cacgttcaaa atcagcaaat cctattaaaa tgccattaga ttctgcaata
     3301 acagcattta aacgtgttct tcctttagta tatacaggtg caaaacttcg tattttttca
     3361 tcacatgaat ttgcatacgg tacttctgtt acttcaaaag atataattga accatatcaa
     3421 accttacttt ttgatgtaga acttgttaaa acattaaaag gaaattatgt tgattcagtt
     3481 cgcgattccc tttataaaga cactgtaaaa aaataataat taataattta tacaaatctc
     3541 atgaaatcac ttgtaaaatt cacaacaatt acaacagtag ctataagttt attagcttgt
     3601 aacggattaa aaaaagacaa agatgcaaaa ttaactaact tacgagacag tgcaagctac
     3661 tgtttaggtt taagtgttgc tacaaattta gcacaagata aattagattc gcttaattta
     3721 gatgcattta tagctggtta tttagacgca aaagacccgg aatcatataa aattgcagtt
     3781 gcagaaataa gtaataccat tcaaaatttt gcaatccaag aaataaaaat taaatattct
     3841 gcaaatatag aagctggtaa aaaattcttg gaagaaaata aaaaacgtgc cggagtaaca
     3901 acaacagctt caggattaca atatgaagta atttcaatgg gaaccggagc aaaaccacaa
     3961 atatatgatt ctgtaacagt aaattatcac gggactaaat tagacggttc tgtatttgac
     4021 agttcaaaag gagctaatcc tgttacctta tcattaactc ccggaaaact tattcaaggt
     4081 tgggttgaag gtatgacatt atttcctgtt gggagtaaat ttaaattata tataccggaa
     4141 gatttagcat atggtgttaa t
//
LOCUS       DAOTOW010000101         4160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17319_length_4160_cov_4.379781, whole genome shotgun sequence.
ACCESSION   DAOTOW010000101 DAOTOW010000000
VERSION     DAOTOW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17319_length_4160_cov_4.379781"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="P5243_02585"
     CDS             complement(<1..333)
                     /locus_tag="P5243_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS18365.1"
                     /translation="MESKEKTIIVAWDFSNVAEYALQHAIRFAQKIESRVVLLNIVDD
                     DKEIENVSNQLNIVVEDALKKYNFKPDYLVKEGSIFTTIKEVANELDALFVFMGTHGI
                     KGLQKHADK"
     assembly_gap    610..619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <620..4078
                     /locus_tag="P5243_02590"
     CDS             <620..4078
                     /locus_tag="P5243_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRS18366.1"
                     /translation="GLLYYNGSEWHIQTWNGKPIMYQGVNNTIYVGGFNTIAELVIDE
                     YNRYTFVSLVDSTADFGQIDKIVQYQNRIYFVANKSLYYISNNQPKKLLSDSKFLSVF
                     TDRNKMFISTSDGLYEFKDEKAETTNISDFFRNNEIIELFRYANVLIARTYDSFYKIK
                     SDNSIEKFITDIDAEVLQYEYSTAQKLSNNILCVATKKNGIYFINENGKLVNYLNTDN
                     GLYDNSIYDLFVDKANNLWAGFSNGVCRIEIPSAFTYFNRSYELHGKVTSIIRHNKYM
                     YIGTESGLYKLVKGESKVSTFQKIFKTSTQCNVMISLKESLLVGTKQGLYEIVGDVPQ
                     LLFQGNIIAIKIHKTSQLLWVATKTHVYILKKNGKSISLVQAIGPFNSEINSIAIEND
                     TVTWVGTYYEGVFQIKQRNGFFDIQKFSSKSGLPKFSQWIEVYETSSGILFSTAVGLY
                     RFDSGIGMFYKDSKITIPLEYVEARVSPIIEDADKNLWVAFKSHGIYENQIAVAWNSG
                     NQDRYTLIVNQFNKLSKFICSTIYPDMNSVVWLGGFDGLVRMDFKELFTKKNNEKTRI
                     HSIYIANDSLLLHNWIQNNKVVFEYKYNSIRFEFTTPIFENPEKILHTYILEGYTKDW
                     SRPKHISHKEFENLPAGSYVFRVKAIDIYGNESPESVFSFRIKQHPLKSWWAFLLYII
                     LFAAVVVLIFRWRSYLFLQEKTKLSKIIRQRTEDLLIEKEKTDTILRNILPEQTAREL
                     KEKGRATSMRFEMATILFADIQGFTKIAEQMPPDTLIDELDKFFLEFDTIVEHHNIEK
                     IKTIGDAYMCAGGIPEKNRTNPIEVVVAALEMQYRVRVLQENLGENLQNYWGLRIGIH
                     TGPVIAGVVGSKKYSYDIWGDSVNIASRMESSGEVGKVNISETTFMLIQDFFDCDYRG
                     KMPVKYKGEIDMYFVKGFKVQFSDNHLRVLPNKSFGHKLALLKFEDLHEIMLERFEKE
                     LPKDLYYHNLKHTIDVIVQVEIIGHEEGINDEDMLLLKTAALFHDAGFLIGYNDHEEL
                     GIQMTRMILPKYNYTEEQIQTICEIITATKLPHNPKNKLEEIMCDADLDYLGRNDYLS
                     VSRDLYKELIEHNVIKKSEMEWNVMQIKFLQHHRFFTASSRNRRNANKNKQIQHLQEQ
                     TRSFKI"
BASE COUNT         1542 a          505 c          682 g         1421 t
ORIGIN      
        1 tttatcggcg tgtttttgta aaccttttat accatgtgtt cccataaaca caaataaagc
       61 atctaactca ttagcaactt ctttgattgt agtaaaaatg ctgccttctt ttaccaaata
      121 gtcgggttta aaattatatt tttttaaggc atcttcaact acaatattta attgattact
      181 tacattttca atttctttat cgtcatctac tatattcaat aaaacaactc gggattcaat
      241 tttttgagca aatctaattg cgtgttgtag agcatattca gccacatttg aaaaatccca
      301 tgcaactatt attgttttct ctttagattc catatgattt attttaattt tttattaatt
      361 ttgaaaaaaa atattccttc tatagtggta aatttgaata gataaaaata atataaaaaa
      421 acattggctc caattaaact gatacttttt tttatttttt tacaattcgg ttttgttttt
      481 tctcaaacaa tagaattggg agtcccttat tgtcaaaata ttacaccaac tgaaataggg
      541 tatggcggag ctatgtattc tatagctcaa gataagcaag gaacaatgtt ttttggaaat
      601 ttcaatggtn nnnnnnnnng gtttattata ttacaatggc agcgaatggc atattcaaac
      661 atggaatgga aaacctatta tgtatcaagg agttaataat acgatttatg ttggaggttt
      721 taatacaata gcagaattgg ttattgatga atataataga tatacttttg tttcacttgt
      781 tgattcaact gctgattttg gtcaaattga taaaattgta caatatcaaa atcgtatata
      841 tttcgtagct aataaatctt tgtattatat ttcaaataat caaccaaaaa aattattgtc
      901 cgattcaaaa tttttatccg tttttaccga tagaaataaa atgtttattt caacttcaga
      961 cggtttatat gaatttaaag atgaaaaagc agaaacaact aacatatcag atttttttag
     1021 aaataatgaa ataattgaat tgtttcggta tgccaatgta cttatagcaa gaacatatga
     1081 ttcattttac aaaattaaat cagataattc aattgaaaaa tttattaccg atatagatgc
     1141 cgaggtcctg caatatgaat atagtactgc tcaaaagcta tctaataata ttttgtgtgt
     1201 tgcaaccaaa aaaaatggca tttattttat aaatgaaaat ggtaaattgg taaattattt
     1261 aaatacagat aatggtttat atgataatag tatatatgat ttatttgtag ataaagcaaa
     1321 taatctgtgg gcaggattta gtaatggtgt gtgtagaata gagattcctt cagcttttac
     1381 ttatttcaat cgcagttatg aattacacgg taaagttact agtataatta gacataataa
     1441 gtatatgtat attggtactg aatcgggatt atataaattg gtaaaaggtg aaagtaaagt
     1501 ttctacattt caaaaaatat ttaaaacatc tacgcaatgc aatgtaatga tttcattgaa
     1561 agaatcttta ttggtaggaa caaaacaagg attgtatgaa attgtaggtg atgtgccgca
     1621 attattgttt cagggaaata taatagctat aaaaattcat aagacttcac aacttttatg
     1681 ggttgcgaca aaaactcatg tgtatatttt aaaaaaaaac ggaaaatcaa tatcattagt
     1741 tcaggctatt ggcccgttta attcagaaat aaattcaata gcaatagaaa atgataccgt
     1801 aacatgggta ggcacttact atgaaggggt ttttcagatt aaacaaagaa atggtttttt
     1861 tgatattcaa aagttttctt ctaagtctgg attaccaaaa ttttcgcaat ggatagaggt
     1921 atatgaaaca tcaagtggta tattattttc aacagctgtt gggttatata gatttgattc
     1981 gggtatagga atgttttata aagacagtaa aattactatt cctttagaat atgttgaagc
     2041 acgagtttca ccaataattg aagatgccga taaaaattta tgggtagcat ttaaatcgca
     2101 tggcatttat gaaaatcaaa tagctgttgc atggaatagt ggtaatcagg atagatatac
     2161 acttattgta aatcagttta ataaattaag taagtttata tgttctacta tttatcccga
     2221 tatgaattct gttgtgtggc ttggtggatt cgacggattg gttcgtatgg attttaaaga
     2281 attattcaca aaaaaaaata atgaaaaaac gagaattcat tcaatatata tagcgaatga
     2341 ttcattgttg cttcacaatt ggattcaaaa taacaaggta gtatttgaat ataaatataa
     2401 ttcaattcga tttgaattta caactcctat ctttgagaat cctgaaaaaa ttcttcatac
     2461 atatatttta gaaggatata ctaaagattg gtcaagacca aaacatattt cacataaaga
     2521 attcgaaaat ttaccagcag gatcatatgt atttagagta aaggctattg atatttatgg
     2581 taatgaaagt cctgaaagcg tgttttcatt tagaattaag caacaccctc tcaaaagttg
     2641 gtgggcattt ttattgtata ttattttatt tgctgctgta gttgttctta tatttcgttg
     2701 gcgttcatat ttgtttttac aagaaaaaac aaaattgagt aaaattattc gtcaacgaac
     2761 cgaagattta ttaatagaaa aagaaaaaac tgatactatt ttacgtaata ttttacccga
     2821 acaaacagct cgtgaattaa aagaaaaagg aagagcaact tcaatgcgtt ttgaaatggc
     2881 aactatttta tttgccgata ttcaaggttt tacaaaaata gcagaacaaa tgcctcctga
     2941 taccctaatt gatgagttag ataaattttt cttagaattt gatacaattg ttgaacatca
     3001 taatatagaa aaaattaaaa caataggcga tgcctatatg tgtgccggag gaatacccga
     3061 aaaaaataga accaatccta ttgaagtggt agttgcagct ttagaaatgc aatatagagt
     3121 aagagttcta caagaaaatc ttggcgaaaa tttacaaaat tattggggat tacgtattgg
     3181 tattcatacc ggtcctgtga tagccggtgt ggttggaagt aaaaaatatt catatgatat
     3241 atggggcgat agtgtaaata ttgccagtag aatggaatca tcgggtgagg tgggtaaggt
     3301 aaatatttct gaaactacat tcatgcttat tcaagatttt tttgactgtg attatagggg
     3361 taaaatgcct gtaaaataca aaggagaaat tgatatgtat tttgtaaaag gatttaaagt
     3421 tcaattttca gataatcatc tacgtgtatt accaaataag agttttgggc ataaattagc
     3481 tttgctcaaa ttcgaagatt tgcatgaaat aatgctcgaa cgatttgaaa aagaattacc
     3541 aaaagattta tattatcata atttaaaaca tactatagat gtaatagtac aggtagaaat
     3601 tataggtcat gaagaaggaa taaacgatga agatatgttg ttgctcaaaa ctgcagcatt
     3661 attccatgat gccggatttt taattggata taatgaccat gaagaacttg gaattcaaat
     3721 gacacgaatg attttgccaa aatataatta taccgaagaa caaattcaaa ctatttgtga
     3781 aattattaca gcaactaaat taccgcataa ccctaaaaat aaattagaag aaataatgtg
     3841 tgatgccgat ttagattatt tagggcgtaa tgattattta tcggtatcgc gtgatttgta
     3901 taaagaactt attgaacaca atgtaataaa gaaaagtgaa atggagtgga atgttatgca
     3961 aattaaattt ttgcaacatc accgtttttt tactgcaagt tctcgaaaca gacgtaatgc
     4021 aaataaaaat aaacaaattc aacacttaca agaacaaaca cgaagtttta aaatttaata
     4081 ataaacaaaa gaatgattga ttggagtaat ttaccgtttg gctatatgcc tacaaatgct
     4141 aatgttaggt gtatatacca
//
LOCUS       DAOTOW010000102         4131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17447_length_4131_cov_3.992149, whole genome shotgun sequence.
ACCESSION   DAOTOW010000102 DAOTOW010000000
VERSION     DAOTOW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17447_length_4131_cov_3.992149"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="P5243_02595"
     CDS             <1..341
                     /locus_tag="P5243_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18367.1"
                     /translation="TAIMTSGNWTLRSEKYETIERGNYGRSHYSTEFDDAVLSNSNTF
                     YIRTYDRFLSLNLTNSGKAVFSNSIKMVDLHALDFNLSVLCYKAGPMQYSHGYIWFPI
                     NIDYKRDVFG"
     gene            552..1145
                     /locus_tag="P5243_02600"
     CDS             552..1145
                     /locus_tag="P5243_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18368.1"
                     /translation="MLHDIYFCGSDTNNLRCVFLVSEDNGSVTCIKAAKVTDDSTWTI
                     TSSVELEKGANKKIEYIDEDNSYCYFSITYLPSVSGLKQYFRISYVTGIIEEMVHSYK
                     STGKFIGIANGKPYFAKYNDTQLESIYYLNNNKWYALPIDGLKKMIPAYMYLKIKPLG
                     RQNKLYFVISATKMLEPYEPLYQVDYYVIEYTESIAK"
     gene            1154..1750
                     /locus_tag="P5243_02605"
     CDS             1154..1750
                     /locus_tag="P5243_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18369.1"
                     /translation="MLNRIKTIFILFFVVSFLNACDKENVTTNEQSVGQNTTISKETE
                     LNLCNCNKHGEVVYGGCKCDPGYTGDSCQFLSANFFTGDFYEQTFGYDITISMYGDTS
                     DAQILFEITKPFVCSFNATIENNTAVIPVQYFCVNADSVEDGGNQVYRYYTIEGYAFA
                     YFNGTTKNINLYLKKTDNNGIEEESYNLYDLYEKSLFQ"
     gene            1766..4045
                     /locus_tag="P5243_02610"
     CDS             1766..4045
                     /locus_tag="P5243_02610"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18370.1"
                     /translation="MNTTLVTPRYSIIILFLFLFIQIQKGYTQLDIKTVFSSNNFYFS
                     NNTSKEYIIKRIDANSVLTLLPEGFYLLYITNNGNTQKAIALDYMRRMSAIHVLDSVH
                     FLVGTDNGRIFYTSNQGETWQQASGINDFYAIKNFAFYDAQYGMAYTSKGRIYRTQDA
                     GKTWQQLPIIESTPNIKTIAYTTKNTAFAVLQFRAHSSGSNFLYKTVNAGATWEPIRL
                     TARYAEYTATASPVFSMEDSLNGLLCVGPNVYTTNDGWKTFTHNIPYPNNDITTKGVY
                     FDNSGKWYIQNFMSEDKGKSWQNSSGYTHMNDNPTTFFDATTNTAWTPTRLYQYTISK
                     YGLNISNNKPFNYWNGLETLSSIAISENQYLITTDEGRLYVSNNSGRTFNYHPQKFNY
                     SLSQSCFIDNKGFIIGYNNENPLKDSLFFSEDNGKTWEIKSLGVSNFIPMKIQTTEKH
                     IFIYGDAKVYGPSNEYISGGYTLLISSDTGKTWINRPLPDSLIGYDQVKFLNENFGWI
                     SSFNGRLYITNNGGKTWIKRFDYNYAIPGIPYSERTYFFTDSLQGWTNGYPFERTFDG
                     GKTWQKCSTHPFPTNNNQVYGLSMFAVDNLNCFISYTVYSTEIPHPQYLAYTNDGGKT
                     WNTEYCYSNINLNNMIPNKNNSFLFVNGNELLELQLPYINNTKTKVNYEQITTYEPSI
                     NKNDFILLKPDSNGITVILLDNKPLKNIKIYTIDGRCIFEQIEIQTSCYINLCNIESG
                     IFFLKGYINNIPQCIGFVR"
BASE COUNT         1498 a          583 c          647 g         1403 t
ORIGIN      
        1 atacggcgat aatgacaagt gggaactgga cattacgttc tgaaaaatat gaaactatag
       61 aacgtggtaa ttatggtagg tctcattatt ctacagaatt tgatgatgct gttttaagta
      121 atagtaatac cttttatatt cgtacgtacg ataggttttt gtcattaaat ttaaccaatt
      181 ccggtaaagc agtcttttca aatagtataa aaatggttga tttgcatgcg cttgatttta
      241 atttatctgt tctttgttat aaggccggac ctatgcagta ttctcacggg tatatatggt
      301 ttccaataaa tatagactat aaaagagatg tatttggttg accacccaaa cctagtgaaa
      361 cttcattcta ttattttaaa gaaggagcaa cacaattaga agaaaggctt tatactaacg
      421 aagtcaactg cagaggtttc gtacaaggca atgtgtattt tgaatgtcct tattggcaat
      481 atgatgcttg tgcttataat ttttctacac gtaaaatttc agatttaaga tacttcccat
      541 ttcatactaa catgttacat gatatttatt tttgtggttc agatacaaat aatctaagat
      601 gtgttttttt ggttagcgaa gacaatggtt cggtgacatg tataaaagct gctaaagtaa
      661 ctgatgactc tacctggaca ataacttcat cagtagaatt agagaaaggt gcaaataaaa
      721 aaattgaata tattgatgaa gataattctt attgttactt ttcaattact tatttgccgt
      781 cggtatccgg gttaaaacag tattttagaa tatcttatgt aaccggtatt attgaggaga
      841 tggtacattc atacaaatca accggtaaat tcataggtat tgccaatggt aaaccatatt
      901 ttgccaaata caatgataca caattggaaa gcatttatta tttaaacaat aacaaatggt
      961 atgctttacc tatagatggg ttgaaaaaga tgatacctgc ttatatgtat ttaaaaatta
     1021 aaccattagg aaggcaaaac aaattatatt tcgttattag tgcaactaaa atgttagaac
     1081 cttatgagcc tttgtatcaa gtagattatt atgttataga atatacagaa tcaattgcta
     1141 aataataaat tgtatgttaa atcgtataaa aactatcttt attttatttt ttgtagtttc
     1201 ttttcttaat gcatgtgata aagaaaatgt aacaacaaat gaacaatcag tagggcaaaa
     1261 tacaacaata tcaaaagaaa ccgaacttaa tttatgtaac tgtaataaac atggtgaagt
     1321 tgtttatggt ggttgcaaat gcgatcccgg ttataccggt gatagttgtc agtttttatc
     1381 agccaacttt tttaccggag atttttatga gcaaacattt ggatatgata tcacaatttc
     1441 aatgtatggc gacacttctg atgctcaaat tctatttgaa ataacaaagc cttttgtttg
     1501 tagttttaat gcaacaatag aaaataatac agccgttata cctgtacaat atttctgtgt
     1561 taatgctgat tctgtagagg atggaggtaa tcaggtatac cggtattata ccatagaggg
     1621 atacgcattt gcttatttta atggtacaac caaaaatatt aatttatatc ttaaaaagac
     1681 agataataac ggcattgagg aagaatctta taatttgtat gatttatatg aaaaatcttt
     1741 gttccaataa aaaaaataat tgcaaatgaa tacaacgtta gttactcccc gatatagtat
     1801 tattatttta ttcttgtttt tatttataca gatacagaaa gggtacacgc agcttgatat
     1861 taaaaccgta ttttctagta ataattttta tttttcaaat aatacatcaa aagaatatat
     1921 aataaaacgt attgatgcaa attctgtatt aaccctattg ccagaaggtt tctatctttt
     1981 atatattacc aataacggga atacacaaaa agcaatagca ttagattata tgagaagaat
     2041 gtctgctatt catgtattgg atagtgtgca ttttttggtt ggaaccgata atgggcgtat
     2101 tttctataca tcaaatcaag gtgagacatg gcaacaagct agtggtatta atgactttta
     2161 tgctattaaa aattttgcat tttacgatgc tcaatatggt atggcttata cttcaaaagg
     2221 gcgtatttac agaacacaag atgccggtaa aacatggcaa cagttaccta ttatagaatc
     2281 taccccaaat attaaaacaa tagcttatac aacaaaaaat acagcattcg ctgttttgca
     2341 gtttagggca cattcaagcg gttcaaattt tttatataaa accgttaatg ccggagcaac
     2401 atgggaacct attcgtttaa cagctcggta tgctgaatat actgcaactg cttctcctgt
     2461 ttttagtatg gaggattcgt taaatggctt gctatgtgtt ggacctaatg tttatacaac
     2521 caatgatggt tggaagacct ttacgcataa tattccatac cctaacaatg acattactac
     2581 aaaaggtgta tattttgata attccggtaa gtggtatatt caaaatttta tgagcgaaga
     2641 caagggtaaa tcatggcaaa attcctcagg gtatactcac atgaatgata accccacaac
     2701 tttttttgat gcaactacaa atacagcatg gactcctaca agattatatc aatatacgat
     2761 ttcaaaatat ggcttaaaca tatcaaacaa taagcctttt aattattgga atggtttaga
     2821 aactctatca tcaattgcta tatcagaaaa tcaatatctt attacgactg atgaaggtcg
     2881 tttatacgtt tcaaataact ccggacgaac atttaattat catccgcaaa aatttaatta
     2941 ttctttaagt caatcatgtt ttattgacaa taaaggattt ataattggtt ataataatga
     3001 aaatccatta aaagattctt tatttttttc ggaagataac ggaaaaacat gggaaataaa
     3061 gtcgcttggt gtatcaaatt ttattccgat gaaaattcaa acaacagaaa aacatatttt
     3121 tatttatggc gatgcaaaag tttatggccc aagcaatgaa tatatttcag gggggtatac
     3181 tttattaatt agctcagata caggcaaaac ctggataaat agaccattac ccgactcact
     3241 aatcggttat gatcaagtaa aatttttaaa tgaaaacttt ggttggattt caagttttaa
     3301 cggtagactt tatattacca ataacggtgg aaaaacatgg ataaaacggt ttgattataa
     3361 ttatgcaata cccggaattc cttattcaga acgtacatat ttttttacag actcattaca
     3421 aggatggact aatggatatc cttttgaacg cacgtttgat gggggaaaaa catggcagaa
     3481 atgttctaca catccattcc caaccaataa taatcaagtg tatggattaa gtatgtttgc
     3541 tgtggataat cttaattgtt ttatttcgta taccgtttac tcaacagaaa ttcctcaccc
     3601 tcaatatctt gcatatacaa atgatggcgg taaaacatgg aatacagaat attgttattc
     3661 aaacataaac ctaaataaca tgattccaaa taagaataat tcatttttat ttgttaatgg
     3721 taatgaatta ttagaattac agcttcctta tatcaacaat actaaaacaa aagttaatta
     3781 tgaacaaatt acaacatatg aaccatcaat taacaagaat gatttcattc ttcttaaacc
     3841 tgattcaaat ggcattactg taatattact agataataaa cctttaaaaa atattaaaat
     3901 ttatactatt gatggacgtt gtatatttga acaaattgaa attcaaacat cttgttatat
     3961 taatttatgt aatattgaaa gtggcatatt ttttttaaaa ggatatatta ataacattcc
     4021 gcaatgtatt ggttttgtta gataactctt aaacatagaa aaataaaaga cacaaacatt
     4081 gttattggtc aaataacaaa atattaaatt acattaatta aatataagaa a
//
LOCUS       DAOTOW010000103         4131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17448_length_4131_cov_3.140579, whole genome shotgun sequence.
ACCESSION   DAOTOW010000103 DAOTOW010000000
VERSION     DAOTOW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17448_length_4131_cov_3.140579"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /gene="gldA"
                     /locus_tag="P5243_02615"
     CDS             <1..926
                     /gene="gldA"
                     /locus_tag="P5243_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRS18371.1"
                     /translation="IQTMSIHIQNLTKTFGTQKAVNNISFEISKGEIVGFIGPNGAGK
                     STTMKIITGYITDFEGSVQVNGLDVRTKAIEVKKSIGYLPEHNPMYLDMYVTEYLLFV
                     ARMYSMKGAIQKQIASIIELTGLQTEQHKKIGALSKGYRQRVGLASVLIHNPQVLILD
                     EPTTGLDPNQIIEIRNVIASVGKEKTVILSTHIMQEVEAICNRVIIINNGSIVANNDS
                     HTIKQSQGNSVTISVEFLETCSKDMLMQIPMVADLIPVSATQWLIKPIDNVDIRQNIF
                     NFAVQNNITVLAMQFQEKTLEQVFQELTKIT"
     gene            938..2557
                     /locus_tag="P5243_02620"
     CDS             938..2557
                     /locus_tag="P5243_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRS18372.1"
                     /translation="MKRLVVLLASFFIGVYTFAQEDTTSLVDVIDDTLSIAEIEPDDF
                     SEVLTDTVIFTQFNSNLDKLVQLWYVQKSEYWKYCNRPLLGYDSSYVPSFTPEEYKQR
                     IAKMNSIIPLSYNDQVQAFINVYAFRKRDRVEIMLGLAEYYFPMFEEILDKYEIPLEL
                     KYLAIIESALNPRATSPVGAGGLWQFMPGTGVMYKLHITSFVDDRRDPYKSTHAAAKY
                     LKSLYGMFNDWLLAIAAYNCGPGNVRKAITRSGGKTNFWEIYPYLPRETRGYVPAFIA
                     AYYVFENHAEHNLYPREIAFPRVTDTIMVTQQLHLQQVSEVLNIPLQFLQDINPQYRV
                     GIIPASEKGHALYLPIDYVTPFLLFQDSILTYKDSLFFDPTVISKSAVTDKHTFHSTT
                     SCKGKVPVYYFVKKGDNLNMIANWYDVSVQDIKTWNKLSSNSIAVDKKLTIYVPANKK
                     DYYAKITTMSLAQKQKLGSAPVTQNVVAKPESTTHKKNTYTYHTVKKGETLWAISQKY
                     DNVSLDDILQMNGLTTKSKITPGMKLKIKTM"
     gene            2575..>4131
                     /gene="asnB"
                     /locus_tag="P5243_02625"
     CDS             2575..>4131
                     /gene="asnB"
                     /locus_tag="P5243_02625"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRS18373.1"
                     /translation="MCGIAGYYSTKHVFQSHELQKMGQVLAHRGPDADGFFSDAFVGL
                     VHRRLKILDLSEEANQPMFSHNKEYIIVYNGEVYNYDDLRREHQIPVTTSSDTEVILS
                     LLAQKGIDACNEFNGMFAIALYNCFEHKLILMRDRMGIKPVFYFWDGSNFAFASEIKA
                     LLQLDFVKQSTSLNHRALQEFLHIGYSGVEETFYTNIFKLPAGSALTIQNNTLSIQQF
                     WNIQAKFQKDCITDYYQAKETLRDLIESSVRLRLKCDVPYGTFLSGGIDSSLVTAVAQ
                     RNISSKLQTFTIAFEHSKYNEAEYARSIANYLGTNHTELLVTEKDAITLVHDILHVYD
                     EPYADSSAIPTMMVSQLARSQVTMTLSGDGGDELFMGYGAYNWAQRLENPLLKIARRP
                     LAFAMKCGNNSLKHRAGMINYPSYDHKPSHIFSQEQGFFSRKEIAQLLVEYTSDAIQL
                     QEYANVCRPLSVRENQAFFDMRYYLCEDLLTKVDRASMRYSLESRVPLLDYTIVEFAL
                     NLDESLKIHNN"
BASE COUNT         1416 a          641 c          719 g         1355 t
ORIGIN      
        1 gaatacagac tatgtcaatt cacattcaaa atcttaccaa aacatttggt actcaaaaag
       61 cggtaaataa tatttcattt gaaatttcaa agggtgaaat agttggtttt attggtccca
      121 atggtgccgg aaaatctact accatgaaga taattaccgg gtatattaca gattttgaag
      181 gaagtgttca agtaaatggg cttgatgtaa gaaccaaagc aattgaggtt aaaaaatcaa
      241 ttggatattt gcccgaacat aatccaatgt acttggacat gtatgttacc gaatatttgt
      301 tgtttgtagc tcgtatgtat agtatgaaag gtgctattca aaaacagata gcttcaatta
      361 ttgaacttac cggattacaa acagaacaac ataaaaaaat tggagcactg tctaaagggt
      421 atcgacagcg tgtaggatta gcttcggtac ttattcataa tcctcaagta cttatacttg
      481 atgaacctac aacaggatta gatcctaatc aaatcattga aattcgtaat gtaattgctt
      541 ctgttggaaa agaaaaaaca gttattcttt caacacatat aatgcaagaa gttgaggcta
      601 tttgtaatag agtgattatt attaataatg gttctatagt agcaaataat gattcacaca
      661 caattaagca gtcgcaagga aattctgtaa ctatttcggt tgaatttctt gaaacatgtt
      721 caaaagatat gttgatgcaa ataccaatgg tagcagattt aattccggta agtgcaactc
      781 agtggcttat taaaccaatt gataatgtgg atattcggca aaatatattt aattttgcag
      841 ttcaaaataa tataactgtt cttgctatgc aatttcaaga aaaaacattg gaacaagtat
      901 ttcaagaatt aacaaaaata acataaaaat tccttgtatg aagcgattgg ttgtcttgct
      961 tgcaagtttt ttcattggtg tatatacatt tgcacaagaa gatactactt ctttagtaga
     1021 tgtaatagat gatactcttt caattgccga aatagaaccc gacgattttt ctgaagtatt
     1081 aacagatacc gtaattttta ctcagtttaa ttctaattta gataagttag tacaactatg
     1141 gtatgtgcaa aaatctgaat attggaaata ttgtaatcgc ccattattag ggtatgatag
     1201 ttcatatgtc ccatcattta cccccgaaga atataaacag cggattgcta aaatgaattc
     1261 tattattcct ctatcataca acgatcaagt gcaggcattt ataaatgtgt atgcatttcg
     1321 taaacgtgat agggttgaaa ttatgttagg attggcagaa tattatttcc caatgtttga
     1381 agaaatactt gataagtatg aaataccact tgaacttaaa tatcttgcaa ttatagaatc
     1441 agctctaaat cctcgtgcaa catcgcctgt tggggcagga ggtttgtggc aatttatgcc
     1501 gggtaccgga gttatgtata aactgcatat cacatcgttt gttgatgatc gtcgtgaccc
     1561 gtataaatct acacatgctg ctgcaaaata tttaaaatct ttgtatggaa tgtttaatga
     1621 ttggttgctt gctattgctg catataattg tggtcccggc aatgttcgta aagctataac
     1681 gcgttcgggc ggtaaaacca atttttggga aatttatcca tacctgccac gcgaaacacg
     1741 tgggtatgta cctgcgttta ttgctgctta ttatgtgttt gaaaatcatg ctgaacataa
     1801 tttataccct cgtgaaattg catttcctcg ggtaaccgat actattatgg ttacacagca
     1861 acttcattta caacaagtat cagaggtgct taatattcct ttacaattct tacaagatat
     1921 taatcctcaa tatagagtag gtataattcc tgcatcagaa aaaggacatg ctttatattt
     1981 acctattgat tatgttactc cttttttgct gttccaagat tcaatactaa catacaaaga
     2041 ttcattattt tttgatccta cagtaatttc aaaatctgca gttacagata aacatacttt
     2101 tcattcaaca acttcttgta aagggaaagt tcctgtgtac tattttgtta aaaagggtga
     2161 taatttaaat atgattgcta attggtacga tgtaagtgta caagatataa aaacatggaa
     2221 taagttgtca agtaactcaa tagcggttga taaaaaatta actatttatg tgccggccaa
     2281 taaaaaagat tattatgcaa aaattactac tatgagttta gctcaaaaac aaaaattagg
     2341 ttctgctcca gtcactcaaa atgttgttgc aaaacccgaa agtactactc ataagaaaaa
     2401 tacatataca tatcacacgg taaaaaaagg tgaaacgtta tgggctattt ctcaaaaata
     2461 tgataacgta agcttagatg atattttgca aatgaatgga cttactacca aatcaaaaat
     2521 aacaccgggc atgaagttaa aaattaaaac tatgtaatta attagtagta tttcatgtgt
     2581 ggtatagcgg gatattattc aacaaagcat gtgtttcaat cgcatgaatt acaaaaaatg
     2641 ggacaagtgc ttgctcatcg aggtcctgat gctgatggtt ttttttctga tgcttttgtt
     2701 ggattagttc atagacgact caaaatactt gatttgtctg aagaggcaaa tcaacctatg
     2761 ttttctcata acaaagaata tattatagtg tacaatggcg aagtttataa ttatgatgat
     2821 cttcgccgtg aacatcaaat acctgttaca acaagttccg ataccgaagt tattctctca
     2881 cttttagcac aaaaaggaat tgatgcatgc aacgaattta atggaatgtt tgccatagct
     2941 ttgtataatt gttttgaaca taagcttatt cttatgcgag atagaatggg tataaaaccc
     3001 gttttttatt tttgggatgg tagcaatttt gctttcgctt cagaaattaa agcattgtta
     3061 caattagatt ttgtaaaaca aagtacatcg ttaaatcaca gagcattaca agaattcctc
     3121 catataggat attccggtgt ggaagaaact ttttatacaa atatttttaa acttccggct
     3181 ggttctgctc tcaccattca aaacaatacc ctatctatac aacaattttg gaatattcaa
     3241 gctaaatttc aaaaagactg tattaccgat tattatcagg caaaagaaac tttgcgtgat
     3301 ttaatagaat catcggtacg tttacgatta aaatgtgatg tgccctatgg tactttttta
     3361 agcggaggta tagattcttc attagttacc gctgtagctc aacgtaatat tagcagtaaa
     3421 ttacaaacat ttaccattgc atttgagcac agtaaatata atgaagccga gtatgctcgc
     3481 agtatagcaa attatttagg tactaatcat accgaattac ttgttactga aaaagatgct
     3541 attacattgg ttcatgatat attgcatgta tatgacgaac catatgccga ttcttctgct
     3601 attcctacta tgatggtttc tcagctggca cgctcacaag ttactatgac gctttcgggt
     3661 gatggcggtg atgaactttt tatgggatat ggcgcatata attgggctca gcgactagaa
     3721 aatccattgc taaaaattgc acgaaggcca ttagcatttg ctatgaaatg tggaaacaat
     3781 agtttgaaac atagagccgg tatgattaat tatccgagtt atgaccataa acctagtcat
     3841 atcttttcac aagaacaagg tttttttagt cgcaaagaaa tagcacaact acttgtagaa
     3901 tatacatctg atgcaataca gttacaagaa tatgcaaatg tatgtagacc tcttagtgtt
     3961 cgcgaaaatc aagcattttt cgatatgagg tattatttat gcgaagattt actaacaaaa
     4021 gttgatagag catcaatgcg atattcatta gaatcgagag tgccattgct tgattatact
     4081 attgtagaat ttgcattaaa tttagatgaa tcattaaaaa tacataacaa t
//
LOCUS       DAOTOW010000104         4116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17519_length_4116_cov_3.226053, whole genome shotgun sequence.
ACCESSION   DAOTOW010000104 DAOTOW010000000
VERSION     DAOTOW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17519_length_4116_cov_3.226053"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="P5243_02630"
     CDS             complement(<1..904)
                     /locus_tag="P5243_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS18374.1"
                     /translation="MSEKLTLPTDVSIITTYRCQMRCKMCNIWEFPSDVKKEIEAKDL
                     EILPQLKFANITGGEPFQRMDLEDIVEVAFKRAERVVISTSGWHYERALKLAERFPNI
                     GIRVSIEGLAERNDDLRGRPGGFDRGLRLLLALSEMGVKDIGFGITVSNNNSDDMLWL
                     YQLSKKMKLEFATATYHNSFYFHKDDNFVTNQDEVIANFKTLIAYLLKENNPKSWYRA
                     FFNLGLINYIKGGKRMLPCEAGNANFFIEPYGDVYPCNGLEDKIWKESMGNIKDYTNF
                     EDLWFSPQATRVRNLVKECPKNCWM"
     gene            complement(916..1575)
                     /locus_tag="P5243_02635"
     CDS             complement(916..1575)
                     /locus_tag="P5243_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRS18375.1"
                     /translation="MVIDDNLVTLQERIHISESLYILRFTPKIFSFKTGQHLSLSLAH
                     DSNKRVYSIASGENENHIDILIREVEHGNLSKKFKKVPIGTVFQLSKPTGYFTLPDNI
                     ENHTIYCIATGSGIAPFISYTQTFPHVPFTIIHGIKNISESFEHNISKDATYISCTSK
                     STEGIFNGRVTEYIKKQTFNTTDLFYICGNGEMIHEVYSFLKLIGIDRNNINYEEYYN
                     N"
     gene            complement(1615..2703)
                     /locus_tag="P5243_02640"
     CDS             complement(1615..2703)
                     /locus_tag="P5243_02640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS18376.1"
                     /translation="MKIFVTGTRGIPNILGGVETHCEHLFPLIADNNTFNITLIRRSS
                     YIQDSNKIQQYKGITLVDIFSPRKKSIEAIVHTCLAITYARIKKADVVHIHAIGPAIL
                     TPFARLLGLKVVFTHHGADYNRQKWGKMAKWILKTGEKWGVKYANHCIVISQTIYNDI
                     QKFNPKRVSLIPNGVTIPEKTLQTHYIESLGLQPQKYMIVVGRFVPEKGFHDMLQAYI
                     SLQTDYKLVFVGDADHESEYSKHIKIDAKKHGVVLTGFIKGEKLHQIFSHAGIFVMPS
                     YHEGLPIALLEAMSYNLPVVVSNISANTEIELPKSSFFEVGNIHDLSEKISQTLSDKQ
                     QIDYSHILTRKYNWHNIARETIQVYSQL"
     gene            complement(2705..3307)
                     /locus_tag="P5243_02645"
     CDS             complement(2705..3307)
                     /locus_tag="P5243_02645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS18377.1"
                     /translation="MRLWHCIYSYQCSLTFSKIYDRIYSAWIRSNIPHMHHSAIIRKG
                     CYVVGGKYMILGKNTMIERHSVLTCWDTYKENKYSPHLEIGNDCSLGEYLHITCIGSI
                     KIGNGVLMGRRVTISDNSHGSTEITDLQIKPTDRPLISKGAIVIEDNVWIGDKATILS
                     GVTIGKGAVVAANAVVTKDVPAYSVVGGIPAQILKQHTSF"
     gene            complement(3340..>4116)
                     /locus_tag="P5243_02650"
     CDS             complement(3340..>4116)
                     /locus_tag="P5243_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS18378.1"
                     /translation="TLRMIYRKISDPLLAHRSDFIMTVSQSSARDIIKYLHVKESKVL
                     VTPNATNKNKFKLYNDESVQLILNKYDLSFKEYLLFVGTIDFPGKNIKVILEAFFELK
                     EKESITEKLVIIGKKGHNAEVIFDYVDKSPYKDQVIFTGYLPDEDLPYLYAGAKIMLY
                     LSLYEGFGLPVLEAMSCGTPVICSNTSCFPEIVEELDVCVSPNDVNAVKEKTVKLIKD
                     DKYRNEIAEQCYKKSIKYNWKDSAKLYNQVFEQTIEKYKK"
BASE COUNT         1273 a          695 c          621 g         1527 t
ORIGIN      
        1 ccatccaaca attttttggg cattctttta caagatttcg aactcttgta gcttgagggc
       61 taaaccataa atcttcaaag tttgtataat ctttaatgtt acccatgctt tctttccaga
      121 ttttatcttc taatccatta caaggatata catctccata aggttcaata aaaaaattag
      181 catttcctgc ttcacatggt aacatccgtt ttccgccttt tatataattt attaagccta
      241 aattaaagaa tgcacgatac cagcttttag gattattttc ttttaataaa taggcaatta
      301 aagttttaaa gtttgcaatt acctcatctt gattagttac aaaattatca tctttgtgaa
      361 agtagaaaga attatgatat gtagcggtag caaattcaag tttcattttt ttcgaaagtt
      421 gatacaacca taacatatca tcagaattgt tattagagac ggttatgcca aagccaatat
      481 cttttacacc catttcgctg agtgccaata ataatcgcaa ccctctgtca aaacctcccg
      541 gacgaccgcg taaatcatca tttctttcag ctaatccttc tatagaaacc cgaatgccaa
      601 tattagggaa tcgttccgct aattttaatg cacgttcgta atgccagccc gaagttgaaa
      661 tcacaacacg ttcggcacgt ttaaatgcaa cttctacaat atcttccaaa tccattcttt
      721 ggaaaggttc tccaccggta atattagcaa attttaattg tggtaaaatt tctaaatctt
      781 ttgcttcaat ttcttttttt acatcacttg gaaattccca tatattgcac attttacaac
      841 gcatttgaca tctataggtt gtaatgattg aaacatcggt gggtaaagtt aatttttctg
      901 acattggagt aaatcttaat tattataata ttcttcgtaa ttgatattgt ttctgtcaat
      961 accaattagt tttagaaatg aatatacttc atgaatcatt tcgccatttc cgcaaatata
     1021 aaacaaatct gttgtattga acgtttgttt ctttatatat tcggttactc ttccattaaa
     1081 aataccttcg gtgcttttgc ttgtgcagct tatgtaggtt gcatcttttg aaatattatg
     1141 ttcaaatgat tctgaaatat ttttaatgcc atgaataatt gtgaacggaa catgtggaaa
     1201 cgtttgagta tatgatatga atggagcaat accactgccg gtagctatac aataaatggt
     1261 atgattctca atattgtcgg gtaaagtaaa gtacccagta ggttttgata attgaaaaac
     1321 agttcctata ggtacttttt tgaacttttt cgacagattt ccatgttcta cttctcttat
     1381 aagaatatca atatgatttt cgttttcccc acttgcaatt gaatataccc gtttattgct
     1441 atcgtgagcc aacgaaagtg ataaatgctg ccctgtttta aacgaaaata tttttggagt
     1501 aaaacgtagt atatataatg attcacttat atgaattcgt tcttgtaacg taacaagatt
     1561 atcatcaatt accatattgt tttttatatt ttgaacgata tgtaattgaa ttacttacaa
     1621 ttgagaatac acttgtatag tttcacgagc tatgttgtgc caattatatt tgcgggttag
     1681 tatatgtgaa taatcaatct gctgcttatc actaagggtt tgactaattt tttcagaaag
     1741 atcatgaata ttaccaactt caaaaaatga acttttgggc aattctattt ctgtatttgc
     1801 agaaatatta cttacaacta caggtaaatt atatgacata gcttcgagta atgctatagg
     1861 taacccctca tgatacgaag gcataacaaa tatgcctgcg tgagaaaaaa tttgatgcaa
     1921 tttttcgcct tttataaaac ctgttaatac tacaccatgc ttttttgcat caattttaat
     1981 gtgcttgcta tattcagatt catggtcggc atcgccaaca aaaacaagtt tataatcggt
     2041 ttgtaaagaa atataagctt gtaacatatc atgaaaacct ttttcgggaa caaatctacc
     2101 cactacaatc atatattttt gtggttgtaa tccaagtgat tcaatatagt gggtttgtaa
     2161 tgttttttcg ggaatagtaa ccccatttgg tatgaggctt acacgttttg ggttaaattt
     2221 ttgaatatca ttgtatatag tttgtgaaat tacaatacaa tgatttgcat attttacacc
     2281 ccatttttcg ccggttttta gaatccattt tgccatttta ccccattttt gtctgttata
     2341 atcagctcca tggtgagtaa atactacttt caaacccagt aagcgtgcaa atggagttag
     2401 aattgcaggt cctatagcgt gtatatgtac tacatcggct tttttaatac gagcatatgt
     2461 aatagctaaa catgtatgaa caatagcttc tatacttttt ttccgaggcg agaagatgtc
     2521 aactaatgta atgcctttgt attgttgtat tttattagaa tcttgaatgt aagaacttct
     2581 tcgaataaga gttatgttaa atgtgttatt atcggcaatt aatggaaata ggtgttcgca
     2641 gtgtgtttct acgccaccta aaatatttgg aattccacgt gtacctgtga cgaagatttt
     2701 catattagaa actagtatgt tgtttaagta tttgggcagg tattcctcct acaacggaat
     2761 aagccggtac atctttagta acaacagcat ttgctgcaac aacagcacct tttccaattg
     2821 taactcccga caatattgtt gctttatccc caatccacac gttgtcttca attacaatag
     2881 caccttttga aataagtggt cggtcggtcg gttttatttg taaatcggtt atttctgtgg
     2941 aaccgtgaga gttatcgctt atagtaaccc tccgtcccat caaaactccg ttgcctattt
     3001 taatagaacc tatgcatgta atatgaagat attctcccaa agaacaatca ttaccaattt
     3061 ctaaatgcgg tgagtactta ttttctttgt atgtatccca acaggttaaa acactatggc
     3121 gttctatcat ggtatttttt ccaagtatca tgtattttcc tcctactaca tagcaacctt
     3181 ttctaataat tgctgaatga tgcatatgag gaatatttga acgaatccat gcagaatata
     3241 ttctgtcgta tatttttgaa aaggtaagtg agcattggta tgaatagata caatgccata
     3301 atctcatttt taagaaaagt agttttgtaa taaattcttt catttcttat atttttcaat
     3361 tgtttgttca aaaacctgat tatataattt tgcagaatct ttccaattat atttaataga
     3421 ttttttataa cactgttcgg ctatttcatt tctgtacttg tcatctttta ttagttttac
     3481 tgttttttct ttaactgcat ttacatcgtt tggagatacg catacatcta attcttctac
     3541 tatttcgggg aaacaagagg tgttagaaca tattacaggc gtgccacacg acattgcctc
     3601 caatacaggt aggccaaatc cttcatacaa tgataaatac aacattattt tggctcctgc
     3661 atacaaatat ggtaaatctt cgtcaggtaa atatccggta aatattactt ggtctttata
     3721 aggtgattta tctacataat caaaaattac ttcggcatta tgaccttttt tacctattat
     3781 aactaatttt tctgtaattg attctttttc ttttaattca aaaaaagcct caagtataac
     3841 tttgatattc tttcccggaa aatcaattgt acctacaaat aacaaatatt ctttaaacga
     3901 taaatcatat ttatttaaaa ttaattgtac tgattcatca ttatagagct tgaatttgtt
     3961 tttattagtt gcgtttggtg taactaaaac ttttgattct ttaacgtgca agtactttat
     4021 aatatctctt gccgatgatt gggaaacagt cattataaag tcacttctat gtgctaataa
     4081 cggatccgaa atttttctgt atatcattcg taatgt
//
LOCUS       DAOTOW010000105         4099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17606_length_4099_cov_3.724283, whole genome shotgun sequence.
ACCESSION   DAOTOW010000105 DAOTOW010000000
VERSION     DAOTOW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17606_length_4099_cov_3.724283"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="P5243_02655"
     CDS             complement(<1..292)
                     /locus_tag="P5243_02655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381656.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRS18379.1"
                     /translation="MADIKPAEVSEILKKQLQGYKSEAELEEIGTVLQVGDSIARIHG
                     LTHVQANEMIEFSNGTKGIVLNLEEDNVGAVLLGQTEGIKEGDIVKRTKQIAS"
     gene            complement(320..862)
                     /gene="atpH"
                     /locus_tag="P5243_02660"
     CDS             complement(320..862)
                     /gene="atpH"
                     /locus_tag="P5243_02660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRS18380.1"
                     /translation="MNQSKISVRYAKAFYEFGSEKNILPSLVDDAKLLSQSFQEIKEL
                     RDFITNPIVKPSDKKIFISSLLSKKVSQHTIDFITMVIMNKRELYIEDMLRNFLEMYR
                     KQSGITGVTLTSTKGFSTAQKNTIISYIEKTYKTKVELFEKIDESLIGGFILRIEDLQ
                     YDASIKTKIQTLKQELLQSN"
     gene            complement(889..1383)
                     /locus_tag="P5243_02665"
     CDS             complement(889..1383)
                     /locus_tag="P5243_02665"
                     /inference="COORDINATES: protein motif:HMM:NF011041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRS18381.1"
                     /translation="MDLVTPGFGLVFWTTITFLILLFLLGKFAWKPIIGAVNARNKSI
                     EDALRLAETTKEEMKQLQADNEKIIAEARRERDALLKEAREMKDQIVAQAKKEAGEES
                     QKILQAAKQSIENEKKNAIQELKTQVAEISIEIAEKILAKELENKKASEDIISNSLDK
                     LKLN"
     gene            complement(1452..1673)
                     /gene="atpE"
                     /locus_tag="P5243_02670"
     CDS             complement(1452..1673)
                     /gene="atpE"
                     /locus_tag="P5243_02670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRS18382.1"
                     /translation="MEEAISLVPLAGIGAGLAVIGAGIGIGKIGGSAMEAIARQPEAY
                     NKIQTAMLIAAALVEGVALFAVVVALISK"
     gene            complement(1713..2822)
                     /gene="atpB"
                     /locus_tag="P5243_02675"
     CDS             complement(1713..2822)
                     /gene="atpB"
                     /locus_tag="P5243_02675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRS18383.1"
                     /translation="MAYNTIKRSFFTLGIVLLFSLFSIASEQEHAQANQAEKGEVDVN
                     KAIFDHIGDNHDWHLWDITHADGTIHPVTIPLPIILINTKPFSITTFMSSEFHHGHSP
                     VTKGKYTYLMHEQKIYYANPDGSLQYDEKGSIISAKPIDISITKNVSTLFLAALLMIL
                     IFTSCAKAYTRRPGLAPKGLQGFMEPIILFVKDDIVIPNIGEKKHAKFLPYLLTVFFF
                     IWILNMIGLIPFFPGSANVTGNIAVTFVLALFTLIITNVNGNKDYWKHIFAMPGLPLW
                     LLPIMIVVELIGVIAKPFALMIRLFANITAGHIVVLSLVSLIFVFQTLAIAPVSVAFV
                     LFMDVLELFVAALQAYIFTMLSALFIGLAVQEHHH"
     gene            3019..3483
                     /locus_tag="P5243_02680"
     CDS             3019..3483
                     /locus_tag="P5243_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS18384.1"
                     /translation="MIIKTATIDDLPAILDLQKVCYLQEAEIYDTTEIPPLKETIKDL
                     EIAFLQGTIFLKLEEKNEIIGSVRAYETDGTCYIGKLIVHPHFQNKGYGTQLLSSIES
                     LFVNCDRFELFTGHKSSKNLYLYTKLGYREFERVFMYDGLELIFLEKNTLEF"
     gene            3487..>4099
                     /locus_tag="P5243_02685"
     CDS             3487..>4099
                     /locus_tag="P5243_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRS18385.1"
                     /translation="MIQLPHNCKALVFDMDGTMIHNKDYHDAAWIEFCNRHSVTIDLD
                     SFCKAYTGKTNEVILQMIFGKKMPKQQILEYETEKEQIYRDLYSPHFMLVSGLQTVLE
                     KAKQKNFLIAIGTSAPVVNVDFVLSHGRIRHFFDVIVHSEMVTHGKPSPEIYLKVAEL
                     LQIQPEQCVCFEDSIAGISSATSAGMYTIGIATELSIADLLKAG"
BASE COUNT         1371 a          643 c          605 g         1480 t
ORIGIN      
        1 tagaagcaat ttgtttggtt cgttttacta tatcaccttc tttgatacct tcggtctgac
       61 ctaaaagtac agctcctaca ttgtcttctt caaggttaag tactatacct ttggtgccat
      121 ttgaaaattc aatcatttca tttgcctgta catgagttag tccatgaata cgagcaatac
      181 tgtcacctac ttgtaataca gttcctatct cttccaattc ggcttcagat ttatagcctt
      241 ggagttgttt tttcaaaatt tccgaaactt ctgccggttt aatatctgcc ataaagtgta
      301 tattatttaa aattcagtac taatttgatt gcaataattc ttgtttcaat gtttgtattt
      361 ttgttttaat acttgcatcg tattgtaaat cttcaattct taaaataaat cctcctataa
      421 gtgattcatc aattttttcg aataattcaa cttttgtttt atatgttttt tctatatatg
      481 aaataatagt atttttttga gcagtagaaa aacctttggt tgatgtaagt gttactcctg
      541 ttattcccga ttgttttctg tacatttcca aaaaattacg aagcatatcc tcaatataca
      601 attcacgttt attcattatt accattgtaa taaaatcaat tgtatgttgt gatacttttt
      661 ttgataataa agaagaaata aatatttttt tatcacttgg ttttacaatt ggatttgtaa
      721 taaaatctcg taattcttta atttcttgaa atgattgaga taaaagtttt gcatcatcaa
      781 caagtgaagg taatatattt ttttctgacc cgaattcgta gaaagcttta gcgtagcgta
      841 ctgatatttt actctgattc atactgctta tatctttaat gcaagacttt aattaagttt
      901 taatttgtct aaagaattag aaataatatc ttccgaagct tttttatttt caagttcttt
      961 agcaagaatt ttttcagcta tttcaattga aatttcggca acttgtgttt taagttcttg
     1021 aatagcattt ttcttttcat tttctataga ttgttttgcc gcttgcaata ttttttgaga
     1081 ttcttcgccg gcttcttttt tagcttgagc tacaatttgg tctttcattt cgcgagcttc
     1141 tttaagcaaa gcatcacgtt cgcgacgagc ctctgcaatt attttttcat tgtcggcttg
     1201 caattgtttc atctcttctt ttgttgtttc cgccaaacgc aacgcatctt ctattgattt
     1261 attacgagca tttactgcac ctattatagg tttccaagca aatttaccaa gtaaaaacaa
     1321 caatataaga aatgttattg ttgtccaaaa tactaatcca aatccgggtg taactaaatc
     1381 catatgtata tttttttaat aatcaatacc aaatgtttgg taacaccaac cgtgttacca
     1441 aacattatac tttacttgct aattaatgct accacaacgg caaataacgc aaccccttca
     1501 acaagagcag ctgcaataag cattgcagtt tgaattttgt tgtatgcttc gggttgacga
     1561 gcaattgctt ccattgcaga accaccaatt ttaccaatac cgataccggc accaattact
     1621 gctaatccgg caccaattcc ggctagaggt actaatgaga ttgcttcttc catgtgaata
     1681 aatatttaaa tattaaacaa ttaattactt ttttaatggt gatgttcttg aaccgctaaa
     1741 cctataaaca acgctgataa catagtaaat atatatgctt gcagagctgc tacaaacagc
     1801 tcaagtacat ccataaacaa cacaaatgct accgataccg gggcaattgc aagtgtttga
     1861 aatacaaaaa ttaacgaaac taaacttaat acaacaatgt gtcctgcggt aatatttgca
     1921 aataaacgta tcatcaatgc aaatggcttt gctattactc caattaattc aactactatc
     1981 ataattggta ataaccatag tggtaatccc ggcattgcaa aaatatgttt ccaataatct
     2041 ttattaccat ttacgtttgt tattattaaa gtaaacaaag ctaaaacaaa cgttacggct
     2101 atattaccgg taacattggc acttccggga aaaaatggaa ttaatcctat catattgaga
     2161 atccaaataa agaaaaatac cgtaagtaaa tagggtaaaa attttgcatg ttttttttcg
     2221 cctatattgg gaattacaat atcatctttt acaaataata taattggttc cataaatccc
     2281 tgcaaacctt taggggctaa acccggtcta cgtgtatatg cttttgcaca cgaagtaaaa
     2341 ataagtatca taagtaaggc tgctaaaaat aatgtactta cattttttgt aattgatata
     2401 tcaataggtt tagcacttat aatagaacct ttttcatcat attgtaatga cccatctgga
     2461 ttagcataat atattttttg ttcatgcatc aaatatgtat atttaccttt agttaccgga
     2521 ctatgaccat gatgaaattc gctagacata aaggttgtaa tagaaaatgg tttggtattt
     2581 ataagtataa ttggtagtgg aatggtaacc ggatgtatag taccatctgc atgagtaatg
     2641 tcccataaat gccaatcatg attgtcgcct atatgatcaa atattgcttt atttacatca
     2701 acttcgcctt tttctgcttg atttgcttgt gcgtgctcct gttccgaagc aattgaaaat
     2761 aatgaaaaaa gaagaacaat acctaatgta aaaaaacttc tttttatcgt attatatgcc
     2821 ataattcaaa atctttttga ttatatactt ctttacatca tacaaaatta attatttttt
     2881 tgagatacac tatttttttc aacatttttt caaaaaaaat aatgaacctg tttttgtgct
     2941 attaaaatta ataatttata ctatattaat gtatatattt gtaaataaca gtgaggacag
     3001 aattcataca ttaaaataat gataataaaa acagcaacta tagatgattt gcctgcaatt
     3061 ttagatttac aaaaagtttg ttacttacaa gaggctgaaa tttatgatac tacagaaata
     3121 ccacccctta aagaaacaat taaagattta gaaattgcat ttttacaagg tacaattttt
     3181 ttaaaattag aagaaaagaa tgaaataata ggctctgtga gagcttatga aactgatgga
     3241 acttgttata taggtaaatt gatagtacat cctcacttcc aaaataaagg atatggaact
     3301 caattattgt cttcaataga atcattgttt gtgaattgtg acagatttga attgtttacc
     3361 gggcataaaa gttcaaaaaa tttatatttg tatacaaaac ttggatatag agaatttgaa
     3421 agagttttca tgtatgatgg attagaatta atttttttag aaaaaaatac tttagagttt
     3481 taaaatatga ttcagttgcc acacaattgt aaagccttag tatttgatat ggatggcact
     3541 atgattcaca ataaagatta tcatgatgca gcatggatag aattttgtaa taggcattct
     3601 gtaactattg atttggattc tttttgtaaa gcatatacag gaaaaacaaa tgaggtaata
     3661 ctacagatga ttttcggcaa aaaaatgcct aaacagcaaa ttttagaata tgaaacagaa
     3721 aaagaacaaa tatatagaga tttgtattct cctcacttta tgcttgtctc cgggttacaa
     3781 acggtgttgg aaaaggcaaa acaaaagaat tttttaattg caattgggac ttcagctcct
     3841 gttgtaaatg tagattttgt tctatctcat ggaagaatca gacatttttt tgatgtaatt
     3901 gttcattcag aaatggtaac tcacggtaaa ccttctcccg aaatttatct aaaagttgcc
     3961 gaattattgc aaatacagcc ggaacaatgt gtttgttttg aagattctat tgccggaatt
     4021 tcttcggcaa cttcagccgg tatgtataca ataggtatag caacagaact ttcaattgct
     4081 gatttactta aagcagggg
//
LOCUS       DAOTOW010000106         4072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17738_length_4072_cov_2.923326, whole genome shotgun sequence.
ACCESSION   DAOTOW010000106 DAOTOW010000000
VERSION     DAOTOW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17738_length_4072_cov_2.923326"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="P5243_02690"
     CDS             complement(<1..1203)
                     /locus_tag="P5243_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18386.1"
                     /translation="MKKILAIVIITLFCIKMYSQQSSRNGIYWPTKDTIRCLVVFVSY
                     YNAPDSTKTIPANANTYFNATMPTDRNQLVGITKYFYEASFGKLVVLGDYVNKVIVVN
                     SSAINNNYNDHLSVFPALDLALANDSTTKSGFSINRHFDNWSYFENGIPKVKTPNKFI
                     DVLVMIHIPSTRYDGSGYTKQTPIISEILDMDGVSIYTSSSRGLDVVRHELAHSLLGG
                     NSYHSGGGNSGERWTLSPMGGYSLLSGSNKNFNSINGWDRWRLGWFSDSAISKKLEIY
                     ALNEQQNLIQSDIDSSIGTQTFILRDFTKYGDAIRIKLPYIGSQSDGLYPVDQWLWIE
                     NHQVLPNTTEALNENGVRPAGIRINYQIGKNDLNYNEPVRNHLITPMVPFGNFDFTYT
                     PEIDIANTD"
     gene            complement(1214..2482)
                     /locus_tag="P5243_02695"
     CDS             complement(1214..2482)
                     /locus_tag="P5243_02695"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRS18387.1"
                     /translation="MKRLQFLFLCIVCSIYSFGQIKVDANNFLKMPDNKSIVFADTWA
                     LEHTTSTSDNGIPVQPGFNLYKYRVANYRFFVSDATSNIGIGTGNPVYGKLEIKTVNY
                     SDEANAVNSGITLNPGTYNSLRMYLCNNIGYITRGGANLQGIAISPEGNVGIKTAPST
                     STNPVIALKVNGAIYASYNNFTGSDKRYKENIKKINFNTIAFDSIHSYTYTYKIKQYS
                     PVFDDSLSMKMTDAVDTRTSFGFIAQEVRNIYPELVIENEEGMLAINYDGFIPILWEA
                     NKEMVRTIAELQKETALLKMQQSRIDDIERSMKTCCSIQEKPKLKSETRVQSEIQDQT
                     KAQAELQQNKPNPFTQNTVIGMYIPQDVQQATVYVYDLKGTQILKLPVFEREQTEITI
                     QAQQLQAGMYIYSLVTDGVLVGSKQMIVTE"
     gene            2746..>4072
                     /locus_tag="P5243_02700"
     CDS             2746..>4072
                     /locus_tag="P5243_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRS18388.1"
                     /translation="MNFDYVNRDISWLYFNYRVLEEAKNKELPLYERLKFLAIYSNNL
                     DEFYRVRVSYYRRLLRDFSEDHPKIKKVQPHAVIEQINTLVSGFQNELMDLFHNEITQ
                     ELEKNQIRLIQPETSLTEAQIQYIHQVFNADILPYLQPVILIKGRIKPFLRTGQIYIV
                     LRMFPKKLGKLSISKKLRRPVYGLLKLPTDHDIARFIELPKDEQDNHCIMFLEDIMMR
                     FFNAIYPGYDISSWYSVKMTRDADLDFEEHDYEELIDIISTISSTRQLGRPNRFQYDY
                     TMPQSVLQYVAKTFELKTEDLVKAGKYHNFRDLFRFPNPKSPELEYPRFTPLLHPKLQ
                     EVRSIIRHIESNEFILHFPYQSFNYFLHYLQEAAHDESVVEIKSTQYRVAYNSAVVKS
                     LIQAAQNGKKVTVFVELKARFDEENNLRFAKEMKRAGINIIYSIPGLKVH"
BASE COUNT         1307 a          725 c          599 g         1441 t
ORIGIN      
        1 atcagtattt gcaatatcta tttcgggagt atatgtaaaa tcaaaattgc caaatggtac
       61 cattggtgtt attaaatgat tccgcaccgg ctcattataa tttaaatcat ttttcccaat
      121 ctgataattt atacgaatac cggcagggcg aactccattt tcgtttaaag cctccgtagt
      181 atttggcaac acttgatgat tttctatcca cagccattga tctaccggat acaaaccatc
      241 tgattgagat ccaatatatg gtaattttat tcttatagca tcaccatatt ttgtaaaatc
      301 ccttaaaata aaagtttgcg ttcctataga actatcaata tctgattgta tcaagttttg
      361 ctgttcattt aaagcataaa tttctaattt tttgctaatt gcagaatccg aaaaccaacc
      421 taatcgccat ctatcccaac catttataga attaaaattt ttatttgaac cggaaagtag
      481 actatacccg cccataggag ataaagtcca tctttcacct gaatttccac ctccggaatg
      541 gtaagaattt ccacctaata atgaatgtgc taattcatgc cttacaacat ctaaccccct
      601 acttgatgat gtatatatag aaacaccatc catatccaaa atctcagaaa ttatcggagt
      661 ttgttttgta tatcctgaac catcatatct tgtagatgga atatgaatca tcacaagaac
      721 atcaataaat ttattaggtg tttttacttt tggtattcca ttttcaaaat aactccaatt
      781 atcaaaatgt ctgtttatag aaaaaccact ttttgtagta ctatcatttg ctaaagctaa
      841 atctaatgca ggaaatactg acaaatgatc attgtaatta ttattaattg cagatgaatt
      901 aacaactata actttattaa cataatcacc caaaactaca agttttccaa atgaagcttc
      961 ataaaaatat tttgtgattc caactaattg atttctatcg gttggcattg ttgcattaaa
     1021 ataggtgttc gcatttgcag gaatagtttt cgtggaatca ggagcattat aatatgaaac
     1081 aaatacaact aaacatctaa ttgtatcttt agtaggccaa tatataccat ttcgagaaga
     1141 ttgctgagaa tacattttta tacaaaaaag agtaattata actatagcaa gtattttttt
     1201 cattaattat atattattct gttacaatca tttgtttact ccctaccaac actccatcgg
     1261 ttaccaaact atatatgtac ataccggctt gtaattgctg tgcttgtatt gttatttctg
     1321 tttgttcgcg ttcaaatacc ggtaatttta gtatttgtgt accttttagg tcatatacat
     1381 ataccgtagc ctgctgcaca tcttgtggaa tatacatgcc tattactgta ttttgtgtaa
     1441 acggattcgg tttattttgt tgtaattctg cttgtgcttt tgtttggtct tgtatttccg
     1501 attgcacacg tgtttctgat tttagtttcg gcttttcttg gatagaacaa cacgttttca
     1561 tacttcgttc tatgtcatct atgcgtgatt gttgcatttt taacagtgct gtttcttttt
     1621 gcaattcagc tattgttcgc accatttctt tgtttgcttc ccataaaata ggtataaatc
     1681 catcataatt tattgcaagc attccttctt cgttttcaat taccaattca ggatatatat
     1741 tccgtacttc ttgtgctata aaaccaaaac ttgttcgtgt atcaacagca tctgtcattt
     1801 tcatagataa agaatcatca aaaacaggag aatattgctt aattttatat gtatatgtat
     1861 atgaatgtat agaatcaaat gctattgtat taaaatttat ttttttaata ttctctttgt
     1921 atcgtttatc tgatccggta aaattattat acgaagcata tattgctcca tttactttca
     1981 atgctattac aggattcgta cttgttgatg gtgccgtttt tatccccaca tttccttcgg
     2041 gtgatattgc aataccttgt aaatttgctc ctcctcttgt tatatatcct atattattac
     2101 ataaatacat tcgcaaacta ttatatgttc ccggatttaa tgttatcccc gaatttaccg
     2161 catttgcttc gtcactataa tttactgttt taatttctaa tttgccatat accggatttc
     2221 ctgttcctat tcctatattt gatgtggcat cacttacaaa aaatctataa ttcgctactc
     2281 tatatttata caaattaaat cccggttgca caggtatgcc attatcactt gtagatgttg
     2341 tatgctccaa tgcccatgta tcggcaaata ctatactttt attatcaggc atttttaaaa
     2401 aattatttgc atctacttta atctgtccaa aactgtatat tgaacaaact atacataaaa
     2461 ataaaaattg tagtcgtttc attgtaatta cattatttaa gggttattgt ttacattaat
     2521 tacctgcaaa agaaaaaaaa aacgaactat acaaattttt acgaataaat tttgcggcaa
     2581 atacgtacat attgcaacta atatgtatta aaaaagaaac aactttcatt acaacctcat
     2641 tcaaatgtta ccaaaatgtt aagaaaacac aataaatata catttctctt tgttttgttg
     2701 ttttttttgt ataattctgc attttaaact attcgaggaa ttgttatgaa ctttgattat
     2761 gtaaatcgcg acataagttg gctgtacttt aattatcgtg tgttggaaga agctaaaaac
     2821 aaagagcttc cactgtacga acggctcaaa tttttagcaa tttactccaa caacctcgat
     2881 gagttttatc gcgttcgggt aagttactat cgccgattgc ttcgcgattt ttccgaagat
     2941 catccgaaga taaaaaaagt acaaccgcat gcagttatag aacaaataaa tacgttagtg
     3001 agtgggtttc aaaacgaatt aatggatttg tttcacaatg aaattactca ggaattagaa
     3061 aaaaatcaaa ttcgactcat tcaacccgaa acttcgctta ccgaagcaca gattcagtat
     3121 attcatcaag tatttaatgc ggatatttta ccgtatttac aaccggtaat tttaattaaa
     3181 ggaagaatta aacccttttt acgtaccgga cagatataca ttgtgcttcg catgtttcct
     3241 aaaaaattag gaaaactaag tatttcaaaa aaattgcgtc gccctgtgta tggcttactt
     3301 aaattgccta ccgaccacga tatagctcgc tttatagaat tacccaaaga cgaacaagac
     3361 aatcattgta tcatgttttt ggaagatata atgatgcggt ttttcaatgc tatttatccc
     3421 ggatatgata tcagttcatg gtatagtgta aaaatgactc gcgatgccga tttagacttc
     3481 gaagaacatg attacgagga acttatcgat attatttcta caatttcgtc gacacgacaa
     3541 ttggggcggc ctaaccgttt tcaatacgat tatacgatgc cacaatcggt attgcagtat
     3601 gtagcaaaaa cgtttgaatt gaaaaccgaa gatttagtaa aagcagggaa atatcataat
     3661 ttcagagatt tattccggtt cccgaacccc aaatcacccg aattggaata ccctcgtttt
     3721 acacccttgc ttcaccccaa attacaagaa gtacggtcta ttattcgtca tattgaatca
     3781 aatgaattta ttcttcattt tccatatcag tcgttcaatt attttttaca ctatttacaa
     3841 gaagctgccc acgacgaatc ggtggttgaa ataaaatcga cacagtatcg tgttgcctac
     3901 aattctgcgg tagtaaagtc gttgattcaa gcagctcaaa atggcaaaaa ggttacggta
     3961 tttgttgaac tcaaagctcg atttgacgaa gaaaacaacc tgcgatttgc aaaggaaatg
     4021 aaacgtgcgg gtatcaatat tatttatagt attccggggc taaaagtaca tg
//
LOCUS       DAOTOW010000107         4036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_17933_length_4036_cov_2.895252, whole genome shotgun sequence.
ACCESSION   DAOTOW010000107 DAOTOW010000000
VERSION     DAOTOW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17933_length_4036_cov_2.895252"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5243_02705"
     CDS             <1..464
                     /locus_tag="P5243_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS18389.1"
                     /translation="GYYIKHDSYYPESHFVDVTKNKESRTVREDITAISFDQMKQDKV
                     AVRLNNLFFDTNKSDLLPYSIPELQRAAKIIQKYQLRVEISGHTDNVGEDDFNMDLSL
                     RRAQAVKDFLVKEGCNPNLFEVKGYGETKPAQTNETLEGRAKNRRVELRFL"
     gene            complement(487..675)
                     /locus_tag="P5243_02710"
     CDS             complement(487..675)
                     /locus_tag="P5243_02710"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRS18390.1"
                     /translation="MGKADKVIRIILAAIGLLLFSTQIIYGVIGIVIFILSIIFIITA
                     ILGFCPLYILFGIKTNKE"
     gene            complement(747..1466)
                     /locus_tag="P5243_02715"
     CDS             complement(747..1466)
                     /locus_tag="P5243_02715"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRS18391.1"
                     /translation="MKIFVYKIIIFCCIVLLFQTCKDSTCIQSNGKEITQNIFVDTFS
                     TVMIYGICDITILPDTINYITIQGGKNHIELCSLQQSKGAIQWYNSATCGFFKQFEHI
                     ALTIHTTNLDSIILYSPCSLITDTLKRNLFLSAQTEMIKAQIIVNNKLTKIDTYYKAG
                     GLLKIKGNTDVCNITANYTIQVDSKYLQTTKAIITNNTIQDMYISVSDSLVAKTSKSG
                     YIYFSGNPQFISTKGNIKPIL"
     gene            complement(1469..2596)
                     /locus_tag="P5243_02720"
     CDS             complement(1469..2596)
                     /locus_tag="P5243_02720"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRS18392.1"
                     /translation="MKKHLFLPSYLHYILYIILKQYLYILLIITCFTYNKTFGQITNE
                     IQTKLIGGTVLPHHSSIAFLLAEYQKGFEIQYNQKNKHITQFDTLYNFPSFGIAYQHI
                     SLGNSLLLGYASQCYATMRISLFSHTKHTISFEQDLGISYIHSSLLKSPYNTALSSPI
                     NFYAGIDFQYTYSITNQHSIISSVELSHISNGKIKTPNLGINSITFGLAYAYSAINNN
                     AYSTKQILWHTHSIYTGLHGFYKSDDFLSTTKYPVISALFEYEYRHTPKYGILLGFDG
                     FYDQSHFALSPLPESTKLKLQSSEYGIHTGLSAHYNNMSIYIATGKYIRTVGKKPPYY
                     SRVGIRYAFNHFLINLSIKSHRTTADFLECGVGIFLFRSHH"
     gene            2855..3100
                     /locus_tag="P5243_02725"
     CDS             2855..3100
                     /locus_tag="P5243_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRS18393.1"
                     /translation="MRIYVGNLDERIEDSHLFEAFSEFGKVEKANIQLDKYTRKSTGY
                     GFVEMPIEEEALFVIAKVNGATWEGKKLIVKKAFDKK"
     gene            3125..3373
                     /locus_tag="P5243_02730"
     CDS             3125..3373
                     /locus_tag="P5243_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRS18394.1"
                     /translation="MNIYVGSLDFKVKEQQLEQAFAAYGDVTSVRIITDKVTRRSKGF
                     AFVEMPNDADAQAAIEGLNGSTIGSRQVIVNESRPKSN"
     gene            3395..3592
                     /locus_tag="P5243_02735"
     CDS             3395..3592
                     /locus_tag="P5243_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRS18395.1"
                     /translation="MAKGKVKWYNVTKGFGFIAKEDGKDIFVHRTGIDRAIAALEPDQ
                     EVTFDEQDGQKGIMAVNVKLI"
     gene            3749..>4036
                     /locus_tag="P5243_02740"
     CDS             3749..>4036
                     /locus_tag="P5243_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRS18396.1"
                     /translation="MDIENSKAQMKKGVLELCILSIISKNDAYASDIIESMRNADLLI
                     VEGTLYPLLTRLKNDGLLTYRWEESKSGPPRKYYTITPLGRDFLHELQGSWN"
BASE COUNT         1472 a          481 c          678 g         1405 t
ORIGIN      
        1 atggttacta tataaaacat gattcatatt atcctgaatc tcattttgta gatgtaacta
       61 aaaataaaga atcgcgaaca gttagggaag atataacagc aataagcttt gaccagatga
      121 aacaagataa agtggcggta aggttaaata atttattctt tgataccaat aaaagtgatt
      181 tgttgccata ttctattcct gaattacagc gagcagctaa aattattcaa aaatatcaac
      241 ttcgtgttga aataagtgga cataccgata atgttggtga agatgatttt aatatggatt
      301 tatcgttgcg aagagctcag gctgtaaaag attttttggt taaagaaggc tgtaatccaa
      361 atttatttga agtaaaaggt tatggcgaaa ctaaacccgc tcaaactaat gaaacgcttg
      421 agggacgagc aaaaaacaga cgtgttgaat tacggttttt ataaaaaaac aaaaatctta
      481 cggttattat tctttattgg tttttattcc aaaaagtata tataggggac aaaatccaag
      541 tatagctgta attataaaaa taattgaaag aataaaaata actataccta taactccgta
      601 aataatctgt gttgaaaata gtaataatcc aattgcagca agtataattc gtattacttt
      661 atctgcctta cccatatttt ttaccatgtt tatattattt tttatcaaat gtaaaaatgc
      721 tgtataggta aagtatgtga tacaagttac aaaattggtt ttatattacc ttttgtagaa
      781 ataaattgag gatttccgga aaaataaata taaccgcttt tacttgtttt ggcaactaaa
      841 ctatcactaa cgctaatata catatcttgt atagtgttgt ttgtaattat agctttggta
      901 gtttgtaaat acttactatc tacttgtatg gtataattag cagtaatatt acatacatct
      961 gtatttcctt taatttttag tagacctccg gctttataat aggtatctat ttttgttagt
     1021 ttattattta caataatctg tgcttttatc atttctgttt gagccgataa aaacaaatta
     1081 cgtttcaaag tatcggtaat taatgagcat ggagagtaaa gaataataga atcaaggtta
     1141 gttgtatgaa ttgtaagagc tatatgttca aattgtttaa aaaaaccaca tgtagcagaa
     1201 ttataccatt gtatagcccc ttttgattgt tgtaatgagc ataattcaat atggtttttt
     1261 cctccttgta ttgttatata attaatagta tccggaagta ttgtaatatc gcatatgcca
     1321 tatatcatta ctgttgaaaa tgtatctaca aaaatatttt gagtaatttc tttaccgttt
     1381 gattgtatac aagtagaatc tttacaagtt tgaaatagta atacaataca acaaaaaatt
     1441 atgattttat atacaaatat tttcatgttt aatgatgaga tctaaataga aaaataccta
     1501 cgccacattc caaaaaatca gctgtggttc tatgactttt aatactgaga tttattaaaa
     1561 aatggttaaa tgcatagcga attcctacac gtgaatagta aggtggtttt tttccaactg
     1621 tccttatata ttttcctgtt gctatatata tactcatatt attgtaatga gcagacaatc
     1681 ctgtatgaat gccgtattca gatgattgta atttaagctt ggttgattca ggtaagggag
     1741 aaagagcaaa atgcgattgg tcataaaatc catcgaatcc taataatatt ccgtattttg
     1801 gtgtatgtct gtattcatat tcaaatagcg ccgaaataac aggatatttt gttgtactta
     1861 aaaaatcatc agatttataa aaaccatgca atcctgtata aatagagtgt gtatgccata
     1921 gtatttgttt tgtagaatat gcattattat tgattgcgga atatgcatat gctaatccaa
     1981 acgtaatact gttaattcct aaatttggtg ttttaatttt accgttggaa atatgcgaaa
     2041 gttctactga tgatataatt gaatgctgat ttgtaataga atatgtatat tgaaaatcaa
     2101 ttcctgcata aaaatttatt ggactactta atgctgtatt gtatggagat tttaaaagtg
     2161 atgaatgtat gtatgatata cctaaatctt gttcaaaact aattgtgtgt tttgtatgag
     2221 aaaataacga aatacgcata gtcgcataac attgcgaagc gtatcctaac agcagactat
     2281 tcccaagaga tatatgttgg taagcaatac caaaagaagg aaaattgtac agtgtatcaa
     2341 attgagtgat gtgtttgttt ttttgattat actgaatttc aaatcctttt tggtattctg
     2401 caagtaaaaa tgctatactt gaatgatgag gtaataccgt tcctccaatg agttttgttt
     2461 gaatttcatt ggtaatttgt ccaaatgttt tattataagt aaaacatgta attatgagta
     2521 atatatataa atattgtttc aaaataatat ataaaatata gtgaagatat gagggtaaaa
     2581 ataagtgttt tttcatattg tacataaaaa aaatcaaata gtcgtgtaaa aaattgattt
     2641 aaatgtgtat atttgcataa aataggataa ataatatttt gtagagtcaa tatattccta
     2701 tttgaaattt aagaaatgtg tgtgtcttta ctacctgttt ttgtcgaaac acaatcagtg
     2761 taatccaatc gatattcttt cattttctta atagtattaa tcaaaattgc aattagcaac
     2821 atttctgatt gtgagtaaac aactatgtgt aaaaatgaga atttatgttg gaaatttaga
     2881 cgaaagaatt gaagatagtc atttgtttga ggctttttca gagtttggta aagtagaaaa
     2941 agcaaatatt caacttgata agtatacccg taaatcaaca ggatatggat ttgtggaaat
     3001 gcctattgaa gaagaagctt tgtttgtaat agcaaaagtt aatggcgcta catgggaagg
     3061 gaaaaaatta atcgtaaaaa aagcatttga taaaaaatag aataacaatt taacacaaaa
     3121 cattatgaac atttatgtag gaagccttga cttcaaagta aaggaacaac aattagaaca
     3181 agcatttgct gcatatggcg atgtaacttc tgtgcgaata attacagata aagtaactcg
     3241 cagaagtaaa ggatttgcgt ttgtagaaat gccaaacgat gctgacgctc aggcagctat
     3301 agaaggttta aacggatcta ctattggtag tcgtcaggtt atcgttaatg aatctcgtcc
     3361 aaaaagtaat taattaacaa aaatagaata caacatggca aaaggtaaag taaaatggta
     3421 taatgttacc aaaggatttg gatttatagc aaaagaagac ggaaaagata ttttcgttca
     3481 cagaacaggt atagatagag caatagctgc tcttgaacct gatcaagaag ttacttttga
     3541 cgaacaagat ggtcaaaaag gaattatggc tgtaaatgtt aaattaatct aattataaaa
     3601 cgaaagtatt ttctacaata taattgattt taaatatcta aaaatccgat acatactaag
     3661 tatcggattt ttttttgttt gtttttttag tactatgtat tgcatagtac taaaaaatat
     3721 atatatttgc attgtaaaca atttacgtat ggatattgaa aattcaaaag ctcaaatgaa
     3781 aaagggtgta ttggaattgt gcattctttc gattatttca aaaaatgatg cgtatgcttc
     3841 tgatattatt gaatctatga gaaatgcaga tttattaatt gtagaaggaa cattatatcc
     3901 gttgcttacg cggttaaaaa atgatggttt gttaacatat agatgggaag agtctaaaag
     3961 tggtccacct cgtaaatatt atactattac tccgttggga cgtgattttt tacatgaatt
     4021 acaaggttcg tggaat
//
LOCUS       DAOTOW010000108         4010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18070_length_4010_cov_3.605563, whole genome shotgun sequence.
ACCESSION   DAOTOW010000108 DAOTOW010000000
VERSION     DAOTOW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18070_length_4010_cov_3.605563"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            600..1544
                     /locus_tag="P5243_02745"
     CDS             600..1544
                     /locus_tag="P5243_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRS18397.1"
                     /translation="MKTINVFFISIFALALVSCGGGKKEQSSGNAQLEGTITIDGSST
                     VYPITEAVAEEFKKIQPNVRVSIGVSGTGGGFKKFGRNEIDINNASRTIKAKEIAACD
                     SSKIEYIGLTIAYDGLAVIANPQNTWLESITVEELKKIWEPAAQGKIMKWNQIRPEWP
                     NKEIHLFGPGVASGTYDYFTEAIVGESGSSRGDFTASEDDHVLVQGVASDMYGLGFFG
                     LAYYEENMNKLKLIGVDNGKGAVKPSLETVASKEYAPLSRPIFIYVNKNAAKREEVKS
                     FVEFYMKNVSVLSKDVGYIPLPDSLYTKELQKFQDFIK"
     gene            1608..2489
                     /gene="pstC"
                     /locus_tag="P5243_02750"
     CDS             1608..2489
                     /gene="pstC"
                     /locus_tag="P5243_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRS18398.1"
                     /translation="MAKPVQERIIELFLKGSAILTVCTTIGILLVLLSESIPFFKEVS
                     IFEFLTDTEWTPLFTNKRFGILPLISGTLLVTLISIIVAVPIGIIIAIFLSEYASPKF
                     RKTVKPMLEILAAVPTVVYGYFALMTVTPILQMIIPNLSGFNSLSAGIVMGIMIIPLV
                     TSLSEDAMYAVPKSLREASFAMGASRLQTAFKVIIPAAFSGIVVSVILAIARAIGETM
                     IVAIAAGQQPRLTFDPTVPIETITAYIVQVSSGDVPHGSLEYKTIYAAGITLFILTFS
                     LNSISFYIRRKFQEKYQ"
     gene            2501..3358
                     /gene="pstA"
                     /locus_tag="P5243_02755"
     CDS             2501..3358
                     /gene="pstA"
                     /locus_tag="P5243_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRS18399.1"
                     /translation="MTNHEKQKLKDSLFSVIGYIATFSGLLILAYFLIDIFIDGFHRI
                     DIDFLTGFPSRKAENAGIFPAWVGSVWILGLTVIFAIPIGIAAGIYFEEYAPKNKITY
                     ILEVNIANLAGVPSIIYGLLGLEVFGRLMGFGGSLLTGALTLTLLILPIIIVATRESL
                     KAVPQSLRDASHALGATQWQTIWYQVLPSSMGGILTGTILALSRAVGETAPLIVIGAL
                     AYVDFIPNSPMDDFSVLPMQIFNWTSRPQQSFIINAAAAIIILLAITFIMNSIAIYLR
                     NRWQKKIQW"
     gene            3381..>4010
                     /locus_tag="P5243_02760"
     CDS             3381..>4010
                     /locus_tag="P5243_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRS18400.1"
                     /translation="MKNKKIIIHSEKVNVYYGDNHVLKDVNLHMPEHTVTALIGPSGC
                     GKSTYLRMLNRMNDYIVDFRMDGTIKIEDIDIYDKSIEVEELRKQVGMVFQKPNPFPK
                     SIYDNVIYGLTIRGVKKKSYLDEVVERSLQQAALWDEVKDSLKKNAMSLSGGQQQRLC
                     IARTLAVEPTIILMDEPTSALDPISSAKIEELIFELKQKYTIIIVTHNMQ"
BASE COUNT         1394 a          551 c          683 g         1382 t
ORIGIN      
        1 aaaggtttta cgctggcact aactggttta attgtatctt gtgcatacga tacttgtatt
       61 aaagctaata agcttagaat acttacaatg gtaatttttc tcataaaatc aatttaatgt
      121 taattttttg gcaaaaaaat agctttaatt aaaataaata ctcaatttaa tgttaaatta
      181 tcgttaacaa ttaaattttt tataattttt gttattaaat atttaatgtg ttaattcttt
      241 attaattatt gatgctggta tagttttatt tatacggtaa ttttaaaatt attatttact
      301 ctatataaga atgtaattat tttattcgaa accacaatac acaaatttat aatagacaat
      361 aaggtaaaac aatatatttg gatatcttaa taacttatta atgtttacac tatcaatttg
      421 caatttcaac tagcttataa tgattaaatt aagtaatttc attttttatt ttttccctat
      481 ttgttaatta taaattaaca ttacggttgt tttcttaaca tttccataac attaaagtaa
      541 ccacaatacg taacaaacaa agtacttttg agcaattaaa tttttaaaag taaaaatgta
      601 tgaaaacaat taatgtattt tttatttcaa tctttgcttt agctcttgta tcatgtggtg
      661 gaggcaaaaa agaacaatca tcgggtaatg cccagttaga aggaactatc acaatagatg
      721 gttcaagtac cgtgtatcct attaccgaag cagtagctga agagtttaag aaaattcaac
      781 caaatgttcg tgttagtatt ggtgtttcag gaaccggagg tggtttcaaa aaatttgggc
      841 gtaatgaaat tgatattaat aatgcatcgc gaactataaa agcaaaagaa atagctgcat
      901 gtgactcaag taaaattgaa tatataggtt taacaattgc atatgatgga ttagcagtaa
      961 tagcaaatcc tcaaaatact tggttagaat ctataactgt tgaggaattg aaaaaaatat
     1021 gggaacctgc tgcgcaaggt aaaattatga aatggaatca aattcgtcca gagtggccta
     1081 acaaagaaat acacttattt ggtcccggtg tagcttcagg tacttatgat tattttacag
     1141 aagccattgt tggtgaaagt ggaagtagtc gtggagattt tactgcaagt gaagatgacc
     1201 atgtattagt gcaaggtgtt gcttctgata tgtatggttt aggatttttt ggattagcat
     1261 attatgaaga aaatatgaat aaactaaaat taattggtgt agataatgga aaaggtgctg
     1321 taaaaccctc gttagaaact gtggcaagta aagaatatgc tcctctttca cgaccaatat
     1381 ttatatatgt aaataaaaat gctgcaaaac gtgaagaagt taaatcattt gttgagtttt
     1441 atatgaaaaa tgtttctgta ttaagtaaag atgtaggata tataccacta cctgattcat
     1501 tgtatacaaa agaattacag aaatttcaag attttataaa atagtacacc aagtattcta
     1561 tgtgttatgt tttaaatgta acacatagaa tataatttca tatctctatg gcaaaaccgg
     1621 tacaagaacg aataatagag ttgtttttaa aaggaagtgc aatacttaca gtatgtacaa
     1681 cgattggaat attattggtg ctattgtctg aatcaattcc tttttttaaa gaggtttcaa
     1741 ttttcgaatt tttaaccgac acagaatgga cgccattgtt taccaataaa cgctttggta
     1801 ttttaccatt aataagtgga actttattag ttacattaat atctataatt gttgcagtgc
     1861 caataggtat tattatagcc atttttctaa gtgagtatgc ttctcctaaa tttagaaaaa
     1921 cggtaaaacc aatgcttgaa atattggcag cagttcctac agttgtttac ggttattttg
     1981 ctcttatgac agtaactcct attttacaaa tgattatacc aaatctatca ggattcaatt
     2041 cattatcggc aggaatagtt atgggaataa tgataattcc attagttaca tctttaagtg
     2101 aagatgcaat gtatgcagta cctaaatcat tacgcgaagc ttcatttgca atgggggcaa
     2161 gcagattaca aacagctttt aaggttataa taccggctgc tttttccgga attgtagttt
     2221 cagtaatttt agcaattgct cgtgctatag gtgaaactat gattgttgca attgctgcag
     2281 gacaacaacc ccggttaaca tttgatccta ctgtacctat tgaaactatt acagcatata
     2341 ttgtacaggt tagtagtggt gatgtgccgc atggatcact agaatataaa actatttatg
     2401 cagcaggaat taccttgttt atacttacat ttagtttaaa ttctattagt ttttatatcc
     2461 gtagaaaatt ccaagaaaaa tatcaataat tcaagtaatt atgacaaatc acgagaaaca
     2521 aaagttaaaa gattcattat ttagtgtaat tggatatatt gctacatttt cagggttact
     2581 tattttagca tattttctta tagatatatt tattgatgga tttcatagaa tagatataga
     2641 ttttttaacc ggatttccat cgcgtaaagc agaaaatgca gggatattcc cggcatgggt
     2701 tgggtctgta tggatacttg gacttactgt aatatttgca atacctatag gaatagctgc
     2761 cggaatttat tttgaagagt atgctcccaa aaataaaata acatatatac ttgaagttaa
     2821 tatagcaaat ttagctggtg taccttcaat tatatatggt ttattaggat tagaggtatt
     2881 tggccgttta atggggtttg gcggaagttt acttaccggt gcattaactc ttacattact
     2941 tattttacca attattatag ttgctactcg cgaatctctg aaagctgtgc ctcaatcact
     3001 acgcgatgct tcacatgctt taggtgctac acaatggcaa accatatggt atcaggtatt
     3061 accatcgtct atgggaggaa ttcttaccgg tactatatta gctttgtctc gtgcggtagg
     3121 cgaaacagct cctcttattg taattggtgc tttagcgtat gttgatttta ttccaaattc
     3181 accaatggat gatttctccg tattacctat gcaaatattt aactggacat cgcgtccgca
     3241 acaaagtttt attataaatg ctgctgcagc aattattatt ttacttgcta taacttttat
     3301 tatgaatagt atagctatat atttgcgaaa caggtggcaa aagaaaatac agtggtaaca
     3361 cttaaaaaat aaacaaaaca atgaaaaata aaaaaattat aattcatagc gaaaaggtca
     3421 atgtatatta tggagataat catgtgttga aagatgttaa tttacatatg cccgaacata
     3481 ctgttactgc tcttattggt ccatctggtt gcggaaaatc aacttatttg cgtatgctca
     3541 atagaatgaa tgattatatt gtagatttta gaatggatgg aactattaaa atagaagaca
     3601 ttgatattta tgataaatca atagaagttg aggaattacg aaaacaagtt ggtatggttt
     3661 ttcaaaaacc aaatcctttc cccaaatcaa tatatgataa tgtaatttat gggttaacaa
     3721 ttagaggtgt taaaaagaaa tcgtatcttg atgaggtagt tgaacggtcg cttcagcaag
     3781 ccgcattatg ggatgaagta aaggattcgt tgaaaaaaaa cgcaatgtct ctttccggag
     3841 gtcaacaaca acgtttatgt atagcacgaa cgcttgcagt ggaacctaca attatattaa
     3901 tggacgaacc cacatctgca cttgatccta tttcgtcagc taaaatagaa gaacttattt
     3961 ttgaattaaa acaaaaatat accataatta tagttactca caatatgcag
//
LOCUS       DAOTOW010000109         3997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18133_length_3997_cov_3.797311, whole genome shotgun sequence.
ACCESSION   DAOTOW010000109 DAOTOW010000000
VERSION     DAOTOW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18133_length_3997_cov_3.797311"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="P5243_02765"
     CDS             complement(<1..178)
                     /locus_tag="P5243_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRS18401.1"
                     /translation="MTVESRYNQRGVSAGKEDVHNAIKNIDKGLYPQAFCKIIPDILG
                     GDPNYCNIMHADGAG"
     gene            complement(194..1900)
                     /locus_tag="P5243_02770"
     CDS             complement(194..1900)
                     /locus_tag="P5243_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRS18402.1"
                     /translation="MSNTEVLQEAGENEKKSLNFLEQIIEADLPYISKVITRFPPEPN
                     GYLHIGHAKSICLNFGLAQQYGGQCNLRFDDTNPTKEDIEYVDSIKEDVRWLGFDWGT
                     NEFYASDYFQQLYDWAVLLIKKGKAYVCHMSAEEVSESRGTPTIPGKESPYRNRSVEE
                     NLSLFERMKNGEFKEGECMLRAKIDMASPNMHMRDPIMYRIRYAHHHKTGDAWCIYPM
                     YDFAHGQSDFIEGITHSICTLEFEVHRPLYEWFLDNIAEPNQLRPKQREFARLNLTYT
                     VMSKRKLLQLVEEKHVSGWDDPRMPTISGLRRRGYTPESIRNFAEKVGVSKRENIIDV
                     ALLEHCIREDLNKTAPRVMAVLQPLKVVITNYPEGKTEILQGKINPEDEQSGYRDIPF
                     GREIYIEQSDFMEDAPKKFFRLSPGNEVRLKYAYIIKCENVIKDLEGNIVEIHCTYDP
                     ETKSGSGTEASQRKVKGTLHWVPVHNCISATVRLYDRLFMVEDPLDVPEGHDFLEHLN
                     PHSLEEVPALIEPSIAHVTQGYRCQFERTGYFCVDKDSTPNSIIFNKTVGLKDSWAKI
                     QK"
     gene            2018..2386
                     /gene="folB"
                     /locus_tag="P5243_02775"
     CDS             2018..2386
                     /gene="folB"
                     /locus_tag="P5243_02775"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRS18403.1"
                     /translation="MGIIELEDMEFYAFHGCYESEKIVGNQFLVQAKLHTNCSYPAST
                     DNIADALNYQTVYNIIEAQMKIPSSLLEHVCKRILDALFAEFPEKLVKARITVSKIAP
                     PMGGKMKAVSVTMQHGNDDL"
     gene            2477..>3997
                     /locus_tag="P5243_02780"
     CDS             2477..>3997
                     /locus_tag="P5243_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HRS18404.1"
                     /translation="MLKKTYTYCFLLLSIVIAVSCSSQKKIIRKADATYENGEYAKAL
                     EYYEKLLETEESKYNQSVYQYRVAECYRMLNNSRKAEVAYRKAIKLKTREKEAQYWYG
                     MMLLKNEKYKEAEEAFTIYKKSQPKDIRAENGILSCSLAVAWTEKPTRYIVDNIKAIN
                     SREHDFSPIYTTKDYSTIYFTSSRTQEGVKQSINQVSGMNFTDIFETRFDRKGNWTVP
                     VKVQDTTINTEFDDGALSISPDGTNLYITLCKYELGKNIGCQIYNSSLKGGSWSASQK
                     IEIVGDSISIGHPAISDDGLELYFSSRMKGGQGGSDIWKVQRVSESSPWGKPINLGPI
                     INTPGDEMFPYIRSNGVLYFSSDGHPGMGGLDIFRAIKDDKGTWSIYNMQYPINSSQD
                     DFGIIFQGNKEIGLFTSNRKGGRGEDDIYSFELPELQIAVKGKIIDASNKKPIADADI
                     TLIGSDGSLVTAQSKKDGTYSFKLQQYTDYIIIGSYQGFLKKKIKFTTNNVYDNTTF"
BASE COUNT         1363 a          700 c          649 g         1285 t
ORIGIN      
        1 ttcctgcacc atcagcatgc attatattgc aataattagg gtcaccaccg agtatatcag
       61 gaattatttt acaaaatgct tgaggataaa gacctttatc tatgttttta atagcattat
      121 gcacatcttc ttttcctgcc gaaacccctc gttgattata tcgtgattct actgtcattg
      181 tttgtattac gtattatttt tgaatttttg cccaagaatc ttttaaccct actgttttat
      241 taaaaattat agaattagga gtagaatctt tatctacaca aaaatatcca gttcgttcaa
      301 actgacaacg atacccttgt gttacatgtg ctatagatgg ttctataaga gccggcactt
      361 cttctaatga atgaggattt aaatgttcta aaaaatcgtg accttcagga acatcaagtg
      421 ggtcttcaac cataaataat ctgtcataca aacgaactgt tgccgaaata caattatgta
      481 ccggaaccca atgtaatgtt ccttttactt ttcgttgcga cgcctctgtt ccactaccgc
      541 tttttgtttc aggatcatag gtgcaatgta tttctacaat atttccctct aaatctttaa
      601 ttacattctc acacttaata atataagcat attttaaacg cacttcatta ccgggcgaaa
      661 gacggaagaa tttttttgga gcatcttcca taaagtctga ttgttcaata taaatttcac
      721 gaccaaacgg aatgtctcta tatcccgatt gttcatcttc gggatttatt tttccctgca
      781 atatttctgt tttaccttca ggataattag ttattacaac tttaagaggt tgcaatactg
      841 ccataacacg aggtgcagtt ttatttaaat cttcgcgaat acagtgttca agtagtgcta
      901 catctattat attttctcgt tttgacacac caactttttc ggcaaaattt ctaatagatt
      961 ccggggtata tcctcgtcgt cgtaacccac taatagttgg cattcgtggg tcatcccatc
     1021 ctgatacatg tttttcttca accaattgta ataatttgcg tttactcatt acagtatagg
     1081 taagattcaa acgagcaaat tcacgttgtt tgggtcgtaa ttgatttggt tctgcaatat
     1141 tatcaagaaa ccactcataa agtggacgat ggacttcaaa ttcaagtgta caaatagaat
     1201 gagtaatacc ttcaataaaa tcactttggc catgcgcaaa atcatacata ggatatatac
     1261 accatgcatc gccggtttta tgatgatgtg catatcgaat tcgatacata ataggatcac
     1321 gcatgtgcat atttggcgat gccatgtcta tcttggcacg caacatacat tcaccttcct
     1381 taaactcacc atttttcata cgttcaaaca aggaaagatt ttcttcaacc gaacgattac
     1441 gataaggact ctctttaccg ggaatagtag gtgttcctct actttcagaa acttcttcgg
     1501 cagacatatg acaaacatat gctttgcctt tttttattaa taaaacagcc caatcatata
     1561 attgttgaaa ataatctgaa gcataaaact catttgtacc ccagtcaaac cccaaccaac
     1621 gtacatcttc tttaatagaa tcaacatatt ctatatcttc ttttgttgga ttggtatcat
     1681 caaaacgcaa attacactgt ccgccatact gctgagccaa accaaaattt aaacatatag
     1741 acttggcatg acctatatgc aggtagccgt ttggttcggg ggggaagcga gttataactt
     1801 tagatatata cggtaaatct gcttcaataa tttgttctaa gaaattcaac gatttttttt
     1861 cgttttcgcc agcttcttgt aaaacttctg tattcgacat tgtttaaata tttttttaca
     1921 aaaataaaat ttctgtacat tttgttgata gtaattttac cgaattacat gttttttttc
     1981 aaaaatgcac taattttcgc aaaaaattaa acaaaatatg ggaattattg aattagaaga
     2041 catggaattt tatgcatttc atggctgtta tgaatcagaa aaaattgtag gcaaccaatt
     2101 tctagtacaa gcaaaacttc atacaaattg ttcatatccg gcaagtaccg ataatattgc
     2161 agatgcgtta aattatcaaa ctgtttataa cattatagaa gcacaaatga aaatcccttc
     2221 atcgctgctt gaacatgttt gcaaaagaat tttagacgca ttgtttgccg aatttcctga
     2281 aaaattagta aaagctcgta ttactgtttc aaaaattgct cctcctatgg gtggcaaaat
     2341 gaaagcagta agtgttacga tgcaacacgg caacgatgat ttataaaatc aaggtacaca
     2401 tcaatatttt atatatattt gcatctaatt aaaaatatag aaataaactt ttcgttaggc
     2461 acgaaaagaa ttgacaatgc tcaaaaaaac atatacatat tgctttctat tgctttctat
     2521 tgtgattgct gtttcgtgtt cttcacaaaa aaaaataatt cgtaaagctg atgctaccta
     2581 tgaaaacggg gaatatgcaa aagctttgga atattatgaa aaattacttg aaaccgaaga
     2641 atccaaatat aatcaatccg tatatcaata tagagttgca gaatgttaca gaatgctcaa
     2701 taattccaga aaagcagagg ttgcatatcg taaagccatc aaattaaaaa cacgcgaaaa
     2761 agaagctcaa tactggtatg ggatgatgtt actcaaaaat gaaaaataca aagaagcaga
     2821 agaagcattt accatatata aaaaatcgca accaaaagat attcgcgccg aaaatggaat
     2881 tctttcgtgc agtttggctg ttgcatggac agaaaaacca acacgatata tagttgataa
     2941 tattaaagca ataaattctc gagaacatga tttttcgcct atatatacca caaaagacta
     3001 ctcaaccata tattttacct catctcgtac tcaagaggga gtaaaacaat ctattaatca
     3061 agtttcggga atgaatttta ctgatatatt tgaaacacgt ttcgacagaa aaggcaattg
     3121 gacagtgccg gtaaaagtac aagatactac tataaatact gaatttgatg atggagcatt
     3181 atcaatttcg cccgatggta caaatttata tattacgctc tgcaaatatg aacttggcaa
     3241 aaacattggt tgtcaaatat ataacagttc tcttaaaggt ggtagctgga gtgcaagtca
     3301 aaaaattgaa atagttggag actccatttc aataggacat ccggcaattt cagatgatgg
     3361 attggaatta tatttttcgt cgcgaatgaa aggcggacaa ggaggtagtg atatatggaa
     3421 agtacaacga gtttcagaat caagcccctg gggtaagcct attaatttag gtcctattat
     3481 taatacacca ggtgatgaaa tgtttccata cattcgcagc aatggagttc tgtatttttc
     3541 atcagatggt catcccggta tgggtggatt agatattttt agagctataa aagatgacaa
     3601 aggaacttgg tctatatata atatgcaata ccctattaat tcatcacaag atgattttgg
     3661 tataatattt caaggcaata aagaaattgg attgtttact tcaaaccgca aaggcggtcg
     3721 aggtgaagat gatatttatt cgtttgaact acctgaactt caaatagccg taaaaggtaa
     3781 aataatagat gcttctaata aaaagccaat tgccgatgcc gatattactc ttataggtag
     3841 cgacggctct ttagttactg ctcaatcaaa aaaagacggc acatatagtt ttaaattaca
     3901 acaatatacc gattacataa taatagggtc atatcaaggt tttttaaaga aaaaaataaa
     3961 gtttaccacc aataatgttt atgataatac tacattt
//
LOCUS       DAOTOW010000110         3992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18157_length_3992_cov_3.142748, whole genome shotgun sequence.
ACCESSION   DAOTOW010000110 DAOTOW010000000
VERSION     DAOTOW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18157_length_3992_cov_3.142748"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="P5243_02785"
     CDS             complement(<1..119)
                     /locus_tag="P5243_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRS18405.1"
                     /translation="MAKKHDLKFTRNIGIAAHIDAGKTTTTERILYYAGVTHK"
     gene            complement(140..616)
                     /gene="rpsG"
                     /locus_tag="P5243_02790"
     CDS             complement(140..616)
                     /gene="rpsG"
                     /locus_tag="P5243_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRS18406.1"
                     /translation="MRKSKPKKRIILPDPKFADTEVTKFVNNLMFDGKKSIAYDIFYG
                     ALEIVEQKKQSQDLSALEVWKKALDNVTPLVEVRSRRVGGATFQIPSEIRPGRRQSIG
                     MKNLILFARKRSGHSMKERLAAEILAAFNEEGAAFKKKEDTHRMAEANKAFSHFRF"
     gene            complement(645..1031)
                     /gene="rpsL"
                     /locus_tag="P5243_02795"
     CDS             complement(645..1031)
                     /gene="rpsL"
                     /locus_tag="P5243_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRS18407.1"
                     /translation="MPTIQQLVRKGRNNIVDKSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHKLQEHSIVLVRGGRVKDLPGVRYHLVRGAL
                     DSSGVEGRLQSRSKYGAKRPKKDQKK"
     gene            complement(1207..1752)
                     /locus_tag="P5243_02800"
     CDS             complement(1207..1752)
                     /locus_tag="P5243_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18408.1"
                     /translation="MHKTDIERKPIAIDTYEFKEKLHKKIEGSEENLLIIDNSIDNVD
                     YKLAQCCKPIKGDEIFGFLTAGGGIKVHRLNCPNAMNMLQMYPYRIIKTRWTKSESSK
                     EFTVGIRIIGNDDVSIVNNITQIISREPQIKMRSIQIQSNDGNFEGQLSLYITDNHHL
                     MKLLDKIKSIKGVHIAERFDV"
     gene            complement(1966..3357)
                     /locus_tag="P5243_02805"
     CDS             complement(1966..3357)
                     /locus_tag="P5243_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRS18409.1"
                     /translation="MLVTKQYIVEIAKPYIKERDIPYIYKAIEILEQQTNQDYAQKVL
                     RLVEIAIQEIGLGGKAIVSICLHSLYQENIISEQTIIEVFGEKVFGIITGLLRVEKID
                     TSKSKIHSENFIKLILSQADDVRVILILIAKKLFLLRNIQLYSEEQQIHLSEEIASLY
                     APISHRLGLYAIKTEMEEMSMKYLHNDIYKTIAKKLAEKKAQRDEYIQTFILPIQQAL
                     ENKGLQFEIKGRPKSIYSIWNKLKKSNVSFEHIYDLFAIRIIIDSKIEDEKNDCWNVY
                     SIVSDIYRPNPNRLRDWISSPKSSGYESLHTTVLGPENRWVEVQIRTKRMDEIAEKGY
                     AAHWKYKEGNSAVGSVSWLARIRNILENPEEDSLEEDNNSRMQLYTDEIFIFTPQGDL
                     RRLRAHATVLDFAYDIHGDIGNSCVGAKVNGKVVPIKHELQNGDKIEILTSKTQTPKR
                     DWLSFVKTSKARQ"
     gene            complement(3414..>3992)
                     /locus_tag="P5243_02810"
     CDS             complement(3414..>3992)
                     /locus_tag="P5243_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18410.1"
                     /translation="SMNSNGLPGSLLYKMPGEKVQIHEGIYKFVTYTIKPEGILTNDR
                     NGIFIQNGYFIGWKQPYDVLLNVGLDLTSNLKNKIYYNVGFEWVSSTQNGALLMRPIF
                     GDLLPSQPITVINDTNECTIFPTCTRDIITLQTTSTIQNISMYNLYGQLVLRKNGNFS
                     TLSLKSLQAGMYICEVTLENGNRHWQKIILQK"
BASE COUNT         1229 a          683 c          507 g         1573 t
ORIGIN      
        1 attttgtgag taacccctgc ataataaagt atacgctcgg tagttgtagt tttaccggca
       61 tcaatatgtg cagcaatacc aatatttctt gtaaatttta aatcgtgttt tttagccatt
      121 gttttaccct aatatattat taaaatctaa aatgtgagaa cgctttattt gcttctgcca
      181 ttctgtgagt atcttctttc tttttgaaag cagcaccttc ttcattgaat gcagctaaaa
      241 tttcagctgc taaacgctct ttcattgaat gtcccgaacg tttacgtgca aataaaatta
      301 aatttttcat tccaattgac tgacgtctac ccggacgaat ttctgacgga atttggaagg
      361 ttgctccacc tactcttcta cttcttactt caaccaatgg agttacatta tcgagagctt
      421 ttttccacac ttccaacgca gataaatctt gactttgttt tttttgttca acaatttcta
      481 atgctccata aaaaatatca tacgcaatac tttttttacc atcaaacatt aaattgttaa
      541 caaattttgt tacttccgta tcagcaaatt ttggatccgg tagaattatg cgtttttttg
      601 gtttactttt tctcattgta tcaatgaatt aaactaatct tatattattt tttttgatct
      661 tttttcggtc tttttgcacc atattttgaa cgtgactgta agcgtccttc aacacctgaa
      721 gaatctaaag ctccacgaac taagtgataa cgaaccccgg gtaaatcttt tacccttcct
      781 ccacgaacca atactataga gtgttcttgt aatttatgac cttctcccgg aatgtatgcg
      841 tttacttctt tttgatttgt taatctcaca cgagcaactt tacgcattgc cgaatttggt
      901 tttttaggag tagttgtgta cacacgcaca caaacaccac gtcgttgagg acaagaatcc
      961 aatgccggag acttactttt atcaactatg ttatttcttc cttttcgtac taactgctga
     1021 attgtaggca taatacttta cttatatctt attattattt atattttttc cctttaaaaa
     1081 cgggttgcaa aggtaaacat tttttttaat tacaaatagg tagactaaaa aaaaatgttt
     1141 tttttataaa ttttgtaatt atttctttac aaataggtta aaatatgaat acaaatacgg
     1201 atgctattac acatcgaaac gttctgctat atgcactcct ttaatcgact ttattttatc
     1261 gagtaatttc attaaatgat gattgtctgt tatgtataat gataattgcc cttcaaaatt
     1321 tccatcattt gactgtattt gtatgcttcg cattttaatt tgaggctctc gagaaataat
     1381 ttgagttata ttgtttacta tactcacatc atcatttcct ataattctaa ttccaactgt
     1441 aaattcttta gaagactctg attttgtcca acgagttttt attattctgt aaggatacat
     1501 ttgcagcata ttcatagcat ttgggcaatt taatctatga acttttattc caccgcctgc
     1561 tgttaaaaaa ccaaaaattt catcaccttt aattggttta caacactgtg ctaatttata
     1621 atctacatta tctatactat tatcaattat caaaagattt tcttcactac cttcaatctt
     1681 tttatgaagt ttttccttaa attcatatgt atcaattgca ataggttttc tttcgatatc
     1741 tgttttatgc aattcatcaa aaaaatcttt taataacgaa agatcaatct tattttgact
     1801 agcagctaca tataattcaa gtgctgtttt aaatccataa aattttagta atttatgaac
     1861 attatcatcg ccaaatttaa ctttattttg agcaaacttt cttagaataa tttctttacc
     1921 cgactcagct tctttatatt caatatcttt taaatatttt ttaattcact gacgtgcttt
     1981 tgatgttttc acaaatgaaa gccaatctcg tttaggagtt tgagttttgg aagtaagtat
     2041 ttcaatttta tctccatttt gtaattcatg ttttatagga actactttgc cattaacctt
     2101 tgctccaaca catgaatttc ctatatctcc atgtatatca taagcaaaat caagaaccgt
     2161 agcatgagct cgcaatcttc gtaaatctcc ttgaggtgta aaaataaata tttcatcggt
     2221 atacaattgc atacgcgaat tattatcttc ttctaaacta tcttcttcag ggttttctaa
     2281 aatattgcgt attcgtgcca accaacttac tgaaccaact gcagaatttc cttctttata
     2341 tttccaatgt gctgcatatc ctttttcagc aatttcatcc attcgcttgg ttcttatttg
     2401 aacttcaacc catctgtttt caggtcctaa tacagttgta tgtaatgatt catacccact
     2461 gcttttaggc gaagaaatcc aatcacgtaa tctattagga tttggtctat aaatatctga
     2521 tactatagaa tatacattcc aacaatcatt tttctcatct tctattttag aatcaattat
     2581 aattctaatt gcaaataaat catatatatg ctcaaaactt acgttgcttt ttttcagttt
     2641 attccaaatt gaatatattg atttaggacg tccttttatt tcgaattgta agcctttatt
     2701 ttctaatgct tgttgaatag gtaaaataaa tgtttgaata tattcatcac gttgtgcttt
     2761 tttttcagct aatttttttg caatagtctt ataaatatca ttatgcaaat atttcatgct
     2821 catttcttcc atctcggttt ttatagcata taaaccaagt ctatgagaaa taggagcgta
     2881 taacgaggct atttcttctg ataaatgaat ttgttgttcc tcagaatata attgaatatt
     2941 acgtaaaaga aataactttt ttgctattaa aataagtata actctaacat cgtctgcttg
     3001 acttaatatt aattttataa agttttcaga atgaattttt gattttgaag tatctatttt
     3061 ctctacgcgt aataaaccgg taattattcc aaaaactttt tcaccaaata cttctattat
     3121 tgtttgttca gaaattatat tttcttgata caatgaatgc aaacaaatgc taacaatagc
     3181 cttccctcct aaacctattt cttgaattgc tatttctact aatcgtaaaa ccttttgtgc
     3241 ataatcttga tttgtttgtt gttcaagaat ttctattgct ttatatatat aaggtatatc
     3301 tctttcctta atatagggtt ttgcaatttc aactatatat tgtttggtta caagcataac
     3361 aaatatttat attgcaatat agtaatgtta ttcattaata aaaattacta tagttacttt
     3421 tgaagaataa ttttttgcca atggcgatta ccgttttcta aagtaacttc acatatatac
     3481 attcctgctt gtaatgattt gagagacagc gttgaaaaat taccattttt tcgcaacact
     3541 aattgtccat acaaattata cattgatata ttttgaattg tagaagtagt ttgtaatgtg
     3601 ataatatcac gagtacatgt tggaaaaatt gtacattcat tggtatcatt aattacagta
     3661 attggttgag agggcaacaa atcgccaaaa attggtctca taagcaatgc accgttttga
     3721 gttgaagaaa cccattcaaa accaacatta taatatatct tgttttttag attactggta
     3781 agatctaaac ctacatttaa taaaacatca tagggctgtt tccaaccaat aaaataaccg
     3841 ttttgaataa atataccatt tctatcattg gtcaaaatcc cttcgggttt tattgtataa
     3901 gttacaaact tatatatgcc ttcatgtatt tggacttttt ctccgggcat tttgtataat
     3961 aagctacctg gtaatccatt tgaattcata ga
//
LOCUS       DAOTOW010000111         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18250_length_3978_cov_3.304869, whole genome shotgun sequence.
ACCESSION   DAOTOW010000111 DAOTOW010000000
VERSION     DAOTOW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18250_length_3978_cov_3.304869"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="P5243_02815"
     CDS             <1..1939
                     /locus_tag="P5243_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HRS18411.1"
                     /translation="IRYKLYQELASNGYKTLYTNTYIPKEKLFSKEFDIEHIIPKARL
                     FDDSFSNKTLEVRQANIDKREETAYDYVSNSLGTEALQQFVSRVESLYKEGKLSKAKY
                     KKLLMKGSEIGEGFIARDLRESQYIAKKAREILLEICRSVVSTSGSITDKLREDWGLI
                     NIMQELNIEKYRAQGLTEQIEGKDGNVKEKIIDWTKRNDHRHHAMDALTIAFTKHNHI
                     QYLNNLNARKNETNKKHGNIIAIEQKETYKDENGKRYFNLPLPNFRSEAKKHLEQILI
                     SYKTKNKVVTRNKNKIKGSSKVQIALTPRGQMHKETVYGKIQQYATKEIKIGPSYDIA
                     LLNQIANKKYRDAVLNRLQQFDNDPNKAFGGKNALTKNPILLVPDKPMPEKVKIVVME
                     DLYTIRKEITPDLVIDKVIDVQIKRILQQRLIEYNNDPKAAFSNLDKNPIWMNKEKGI
                     SIKRVTISGVSNAEALHYKKDHTGKPIFDAEGNYIPADFVSTGNNHHVAIYRDESGNL
                     QEEVVSFYEAVARVNAGMPIIKKQHENGWQFLFTMKQNEMFVFPSTNFDPTEIDLLNP
                     DNYHLISPNLFRVQKFSKVIYGNSAVRDYVFRHHLETTINDNKQLKDIAYKSIKSLSF
                     FERIIKVRINHLGRIVQVGEY"
     gene            2097..2687
                     /locus_tag="P5243_02820"
     CDS             2097..2687
                     /locus_tag="P5243_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18412.1"
                     /translation="MHFEPGIVYHIYNQGNNRQKLFFNRDNYLFFLQKIRTQLTPHAD
                     VLAWCLMPNHFHLMVLAKETATTRSATLSRAPSIGLNKAIGIVLASYTRAINNQENTT
                     GSMFRSKTKAIPLNQIHGITPAYFNTQFGTKITVTTPQKEYLNICFNYIHNNPVKAQL
                     VRHITDWEFSSAQDYAGLRNGTLIQKQKAREVGLVW"
     gene            2745..3656
                     /gene="cas1"
                     /locus_tag="P5243_02825"
     CDS             2745..3656
                     /gene="cas1"
                     /locus_tag="P5243_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HRS18413.1"
                     /translation="MIKRTLYFGNPAYLSTKQEQLVINLPDAQGFDNKSGLNTIPIED
                     IGVVILDNQQITITHGVIEKLLANNAAVITCNSTHHPTGLLLNLDGNTIQQERWENQI
                     GASEPLKKQLWQQTIQAKIRNQAAVLAQQGIDVQNMKHWIQQVQSGDAANHEARAAAY
                     YWANVFPKQLGFKRGREEAPPNNILNYGYAILRAITARSLVASGMLPTLGIHHHNRYN
                     SYCLADDIMEPYRPYVDMVVLQLIQQGEDYTQLSKPIKTELLQIPTIDVTIEGRKSPL
                     MIALQQTTATLAKCFDKSLRKISYPEL"
     gene            3699..>3978
                     /gene="cas2"
                     /locus_tag="P5243_02830"
     CDS             3699..>3978
                     /gene="cas2"
                     /locus_tag="P5243_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRS18414.1"
                     /translation="MHSRFNAYRIMWVMVYFDLPTETKKERKEAALFRKNLMKDGFTM
                     FQYSIYVRHCPSKENAEVHIKRIKNFLPETGHVCIMYITDKQFGDIQIF"
BASE COUNT         1543 a          726 c          659 g         1050 t
ORIGIN      
        1 tattcgttac aaattatacc aagaacttgc ttcgaacgga tataaaacgc tgtatacaaa
       61 tacatatatc cctaaagaaa agcttttctc caaagaattt gatattgaac acattatacc
      121 aaaagctcga ttatttgatg atagtttttc aaacaaaaca cttgaagtac gacaagcaaa
      181 tattgataaa cgagaagaaa ccgcctatga ctatgtctca aattctttag gtacagaagc
      241 attacaacaa tttgtatcaa gagttgaatc attatacaaa gaaggtaaat taagtaaagc
      301 gaaatacaaa aagcttttga tgaaagggtc tgaaattgga gaaggtttta ttgcacgtga
      361 tttacgagaa agtcaataca ttgcaaagaa agcaagagaa atactattag aaatatgtcg
      421 ttcggttgta tctacaagtg gtagtataac cgataaactt cgtgaagatt ggggattaat
      481 taacataatg caagaattaa atattgaaaa atatagagca caaggtttaa ccgaacaaat
      541 tgagggtaaa gatggtaatg taaaagaaaa aattattgat tggacaaaac gtaacgacca
      601 ccgacatcat gctatggatg ctcttacaat tgcttttaca aaacataacc atattcaata
      661 tttaaacaat ttaaatgctc gtaaaaacga aaccaataaa aaacatggga atataatagc
      721 aatagaacaa aaagaaactt acaaagacga aaatggaaaa agatatttta atctaccatt
      781 gccaaacttt agaagcgaag caaaaaaaca tttagaacaa attctcatat catataaaac
      841 aaagaataaa gttgtaactc gtaataaaaa taaaattaaa ggtagttcta aagttcaaat
      901 tgctcttacc ccacgcggac aaatgcacaa agaaaccgtg tatggtaaaa tacaacaata
      961 tgctacaaaa gaaattaaaa ttggaccttc gtacgatatt gcattattaa atcaaattgc
     1021 aaataaaaaa tatagagatg ctgtacttaa tcgattacaa caatttgaca acgacccgaa
     1081 taaagctttt ggtggtaaga atgcacttac aaaaaatcca attttactag taccggataa
     1141 acctatgccc gaaaaagtaa aaatagtagt tatggaagat ttgtatacta ttcgtaaaga
     1201 aattacgcct gatcttgtta ttgataaagt aattgatgtg caaattaaac gaattttaca
     1261 acaacgatta atagagtata acaacgaccc caaagctgca ttttccaatc tcgataaaaa
     1321 tcctatatgg atgaacaaag aaaaaggtat ttctataaaa cgagtaacca tcagtggtgt
     1381 aagtaatgcc gaagcattac actataaaaa agaccataca ggcaaaccta tttttgatgc
     1441 agaaggtaat tatatacccg cagattttgt tagtaccggc aataaccacc atgttgctat
     1501 ctatcgcgac gaatcaggaa atttacaaga agaagttgta tcattttacg aagcggtggc
     1561 acgtgtaaat gcaggaatgc caattataaa aaaacaacac gaaaatggct ggcaatttct
     1621 gtttaccatg aaacaaaacg aaatgtttgt attcccatct accaacttcg accctactga
     1681 aattgattta ttaaatcctg ataattatca tttgataagt ccaaatttat ttagggtgca
     1741 aaaattttca aaagtaatat atggtaattc tgctgttaga gattatgttt ttagacatca
     1801 tttagaaaca acaattaatg ataataaaca attaaaagac attgcttata aatcaattaa
     1861 aagtctttca ttttttgaaa gaataataaa agtccgtatt aatcatcttg ggcgaatagt
     1921 tcaagttggt gaatattaat aaaaaaacag gaactcaaac ctcacccaac taaattaaag
     1981 caaaaaccta ttcacagggt tacgtaaagt caccccgtga ataaaaaaaa ctacaagcct
     2041 cacaaacaac aaaattcttg tgtatatttg cagtgcaaca ctaacatttt caggcaatgc
     2101 attttgaacc gggaatcgta tatcatatct ataatcaagg gaataatagg caaaaactgt
     2161 ttttcaaccg cgacaattac ttgttttttc tacaaaaaat acggacacag cttaccccac
     2221 atgccgatgt gttagcgtgg tgtcttatgc ctaatcattt tcatttgatg gtgttagcta
     2281 aagaaactgc tactactcgg agtgcgactt tgagtcgcgc tccgagtata ggcctcaaca
     2341 aagcaatagg aatagtatta gcatcataca cccgagcaat aaacaatcaa gaaaacacaa
     2401 ccggctcaat gtttcgcagc aaaaccaaag ccatcccact caatcaaata cacggcatta
     2461 cccccgcata tttcaataca cagtttggca cgaaaattac tgtaacaact ccccaaaaag
     2521 aatatctaaa tatttgtttc aactatatac acaacaatcc ggtaaaagct caattggtac
     2581 gacacataac agattgggag ttttcatcgg ctcaagatta cgcaggcttg cgtaatggta
     2641 cattaattca aaaacagaag gctcgtgaag ttggtttagt ttggtaataa ccctactcat
     2701 cagatgattt tgagtcatct gatgagtact taaactatac tactatgata aaacgaactc
     2761 tctacttcgg aaatcctgcg tacctcagta ccaagcaaga acaattagta attaacctac
     2821 ccgatgcaca aggcttcgac aacaaatcgg gcttaaacac cattcccata gaagatatag
     2881 gtgtggtaat tctcgacaac caacaaatta ctataaccca tggtgtaata gaaaaattgt
     2941 tggcaaacaa tgctgcggta attacgtgca acagcaccca ccaccctacc ggattattgc
     3001 tcaatttaga tggtaatacc attcaacaag aacgctggga aaatcaaata ggtgcaagtg
     3061 aacctctcaa aaaacaattg tggcaacaaa ctattcaggc aaaaatcaga aatcaagctg
     3121 cggtgttggc acaacaggga attgacgtac aaaacatgaa acattggata caacaagttc
     3181 aaagtggcga tgccgcaaac cacgaagcac gagctgcggc atactattgg gcgaatgtat
     3241 tccccaagca actggggttt aaacgcggac gcgaagaagc tccgccaaac aatatactaa
     3301 actacggtta tgcaattttg cgagctatta ctgctcgttc gttagttgca tcgggcatgt
     3361 tgcctaccct tggcatacat catcacaacc gctacaattc ctattgtctt gccgacgata
     3421 ttatggaacc ataccgcccc tatgttgata tggtagtatt acaattaata caacaaggcg
     3481 aagactacac acagttgagc aaaccaataa aaacagaact attgcaaatt cctaccatag
     3541 atgtaacaat agaaggacgc aaaagtccgc ttatgatagc actacaacaa actactgcaa
     3601 ctttagcaaa gtgctttgac aaaagcttga gaaaaataag ttatcctgaa ttataattta
     3661 gtcaaattag tcatcagatg actaaaaaat aaaaacctat gcattcacga tttaacgcct
     3721 atagaattat gtgggtaatg gtatattttg atttaccaac cgagactaaa aaggaacgga
     3781 aggaagcggc tttgtttcgc aaaaatttaa tgaaagacgg gtttaccatg tttcagtata
     3841 gcatatatgt tcggcattgc cccagtaaag aaaatgccga agtacacatt aaacgcataa
     3901 aaaacttttt gcccgaaacc gggcatgtat gcataatgta cattaccgac aaacaattcg
     3961 gcgatataca aatattta
//
LOCUS       DAOTOW010000112         3961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18331_length_3961_cov_2.923707, whole genome shotgun sequence.
ACCESSION   DAOTOW010000112 DAOTOW010000000
VERSION     DAOTOW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18331_length_3961_cov_2.923707"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="P5243_02835"
     CDS             <1..336
                     /locus_tag="P5243_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18415.1"
                     /translation="YVHFTIQYTKQIYDTLDIYVVGGFNSWSITDENKMIYNNQTNMY
                     EAAILLKQGYYDYSLAFVSHDKKHIFTHEIEGSYNKTENRYDIFVYYYDISKGYDRII
                     GYQTIEQTK"
     gene            342..929
                     /gene="yihA"
                     /locus_tag="P5243_02840"
     CDS             342..929
                     /gene="yihA"
                     /locus_tag="P5243_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRS18416.1"
                     /translation="MIIQSATFVTSSTKIEQCPNKQLPEFAFIGRSNVGKSSLINMLT
                     NNHKLAKTSGTPGKTQTINHFLINNSWYLVDLPGYGFAKVSKQNRGEFAKIIQDYVLN
                     RQELVCLFVLIDSRLEPQTIDLEFLKLLGKNSIPFSIIFTKTDKLKPGAIERNIETYK
                     KTLLIEWEELPPIFQSSSSRKHGKEEILQFLENLL"
     gene            987..2423
                     /locus_tag="P5243_02845"
     CDS             987..2423
                     /locus_tag="P5243_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRS18417.1"
                     /translation="MHLGNLCNILIRIVGILSVDISISLLLALYYNEPIIPFIDTIVI
                     CCIISILCGSAIYYSKHKEVTKKESFFIVTFSWFFIAGIGSLPYIFSHYFSIVDAFFE
                     STSGFSTTGSSILSDIEALPKSILFWRSLTHWIGGIGIIVIVIIIMPSLKIGGNNLFS
                     VESSVGEKILPKITSVGKRLLLIYLSLTTLEIIFLLLGDMTLFDSICHAFGTVATGGF
                     STKNTSIADYSPYIQYVIAIFMFLAGINFILYYYLVKNRFDKIKHNNELWFYIKVVIS
                     TTILVTVLLYSQTSRPLEQSFRDSFFQVVSIITCTGFASSDYLLWPQAGSIIMFLLLF
                     AGGCTGSTAGGIKMARHALVFKNLRNIFTKNLHPQVITPVRFNNTIMNEKQNVAVLTF
                     IVWYFIAFTVGTVLLIFLNVDGATAASAIATAMGGVGPGIGTIGPVSNFSHLSNIVKI
                     IISLFMILGRLELYSFLVLLTPAFWKSK"
     gene            complement(2432..3145)
                     /locus_tag="P5243_02850"
     CDS             complement(2432..3145)
                     /locus_tag="P5243_02850"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRS18418.1"
                     /translation="MTVLSVNINKIALIRNSRGANNPNILKVAQDCEQFGAHGITIHP
                     RPDQRHAKYQDVYDLKQILTTELNIEGYPDSKFIDVVCNAKPTQVTLVPDAPDALTSN
                     TGWDTKLHYAFLTDITKQLQSHGIRVSIFVNPEEIFIEYAAKIGTNRIELYTEPYAAR
                     FLHNKNKAVEPYVKCSQYAHSCGLEVNAGHDLNLDNLQYFKESLPVLHEVSIGHALVC
                     DALYLGLEKTIQEYLKRLA"
     gene            3207..3875
                     /locus_tag="P5243_02855"
     CDS             3207..3875
                     /locus_tag="P5243_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18419.1"
                     /translation="MIALSLISENYIPLKDTDSEKIAYDSMFNTRLFHLPVIDENNMF
                     LGMLCEEDISNKPSNSLLLKDIQPKLIQTKVYAHQHIYDIIEKTFNQELSCIAVVDDN
                     NTYLGLIPCSTLTKYFANITSLQQPGAIIVLEMHTKDYTLQKITQIIEENSAKIISLY
                     TTIIPDSTKIELTIKLNTHDIGEIIAELNRFDYTIKTYFEGYSKLHELYKNRIDNLTH
                     YLNI"
BASE COUNT         1463 a          575 c          554 g         1369 t
ORIGIN      
        1 tatgtacatt ttactattca atatacaaag caaatatatg atacgcttga tatatatgta
       61 gttggaggtt ttaacagttg gtctattact gatgaaaaca aaatgatata taataatcaa
      121 acaaatatgt atgaagcagc tattttatta aaacaaggat actatgatta ctcgttagcg
      181 tttgtttcgc atgataaaaa acatatattt acacacgaaa ttgaaggtag ttataataaa
      241 actgaaaaca gatatgatat ttttgtttat tactacgata tttctaaagg atatgacaga
      301 ataataggtt atcaaacaat agaacaaaca aaataaaaac aatgataatt caatcagcaa
      361 catttgttac aagcagtact aaaatagaac aatgccctaa caaacaatta ccggagtttg
      421 catttattgg cagatcaaat gttggtaaat cttcattgat aaatatgcta accaacaatc
      481 ataagctggc taaaacatca ggtacaccgg gcaaaacaca aactatcaat cattttttaa
      541 tcaataattc atggtactta gtagatttac ccggatatgg ttttgcaaaa gtaagtaaac
      601 aaaatcgagg agaatttgca aaaattatac aagactatgt acttaacagg caagaattag
      661 tatgtttatt tgtacttatt gatagtagac tcgaaccaca aacaatagat ttagaattct
      721 taaaacttct tggaaagaat agcattccgt ttagtattat ttttacaaaa accgacaaat
      781 taaaaccggg agctattgag cgaaatattg aaacctataa aaaaacatta cttatagaat
      841 gggaagaatt gcctcctatt tttcaatcat catcttcacg caaacatgga aaagaagaaa
      901 tattacaatt tctcgaaaat ttactgtaat ttttaaaaat cacataatat attctgtaat
      961 tttgcttaga ttttaatata acttacatgc atctaggtaa tttatgtaat attctaatac
     1021 gaattgtagg aattttaagt gttgatataa gtattagttt actattagct ctctattata
     1081 atgagcctat tattccattt attgacacaa tagtaatttg ttgtataatt tctatactat
     1141 gtggttctgc aatttactat tcaaaacaca aagaagtaac aaaaaaagaa tcgtttttta
     1201 tagttacctt ttcatggttt tttatagcag gaattggttc tttaccgtat atattttcac
     1261 attattttag catagttgat gccttttttg aatctacatc aggatttagt accacaggtt
     1321 catcaatatt atcagacatt gaagcattac caaaatcaat tctattttgg agaagtttaa
     1381 cacattggat tggaggaatt ggaattatag taatagtaat tataattatg ccatcattaa
     1441 aaattggcgg aaataatcta ttctctgtgg aatcatctgt tggagaaaaa atattaccca
     1501 aaataacatc tgttggaaaa agattgctgc ttatttatct tagtcttaca actttagaaa
     1561 ttatattttt attattgggt gatatgacat tatttgatag tatttgtcat gcatttggta
     1621 cagttgcaac aggtggcttt tcaacaaaaa acacaagtat agcagattat tcaccgtata
     1681 tacaatatgt tattgcaatt ttcatgtttt tggcaggaat aaattttata ttgtattatt
     1741 atttagtaaa aaatagattt gacaaaataa aacataataa tgaattgtgg ttctatatta
     1801 aagttgtaat cagtacaact atattagtaa ctgtactatt atattcacaa acctctcgcc
     1861 cacttgaaca atcatttaga gattcatttt ttcaagtagt ttcaattatt acatgtaccg
     1921 gatttgcttc aagtgattat ttattatggc cccaagcagg aagtataatt atgtttttgt
     1981 tattatttgc tggcggctgt acaggttcta ccgccggtgg tattaaaatg gctcgacacg
     2041 cactcgtatt caaaaattta cgaaatattt ttacaaaaaa tttacatcca caagttatta
     2101 caccggtacg ttttaacaat actataatga atgaaaaaca gaatgtagca gtattaacat
     2161 ttatagtatg gtattttatt gcatttactg taggtactgt actgttaatt tttctaaacg
     2221 ttgacggagc aactgcagca agtgccatag ctactgccat gggaggtgtt ggacccggta
     2281 taggaacaat aggtccggtt tcaaactttt cgcatttatc aaatatcgta aaaataatta
     2341 tatcactatt tatgatatta ggacgattag agctatattc atttttagtt cttttaacac
     2401 ctgctttttg gaaatcaaaa taacataaaa attacgctaa tcgttttaaa tattcctgta
     2461 ttgttttttc taatcctaaa tacaatgcat cacacaccaa tgcatgacct atagatactt
     2521 catgcaatac aggtaaagac tctttgaaat attgtaaatt atctaaattc aaatcatgac
     2581 ctgcatttac ttctaaacca cacgaatgag catattgaga acacttcaca tatggctcta
     2641 cagctttatt tttattgtgc aaaaatcttg cagcataagg ttctgtatat aattctattc
     2701 tattggtacc aattttagca gcatattcta taaaaatttc ttcaggattt acaaatattg
     2761 aaactcgtat tccatgtgac tgtaactgtt ttgtaatatc agtaaggaaa gcatagtgta
     2821 attttgtatc ccaaccggta tttgatgtta aagcatcagg agcatcagga accaacgtaa
     2881 cttgagtagg tttagcatta catactacgt caataaactt tgaatcagga tagccttcaa
     2941 tatttaattc ggtagtaagt atttgtttca aatcatacac atcttgatat tttgcatgac
     3001 gttgatcggg acgagggtga atagtaattc catgagcacc aaattgttcg caatcttgag
     3061 caaccttaag tatatttgga ttatttgctc cacgagaatt tctaattaag gcaattttat
     3121 ttatatttac acttaaaact gtcatattaa ttgtttttat aaatttagcg tgtaaattta
     3181 caatttaaaa ttaattatta tactacatga ttgcactttc acttatatct gaaaattata
     3241 ttcctctgaa agataccgat tcagaaaaaa ttgcatatga tagtatgttt aacactcgcc
     3301 tatttcatct accggtaata gatgaaaaca atatgttttt aggaatgctt tgtgaagaag
     3361 atatatcgaa caaaccttct aactcattat tattaaaaga tatacaacct aaattaattc
     3421 aaacaaaagt atatgcacat caacatatat atgatattat agaaaaaact tttaatcaag
     3481 aattatcttg tatagcagta gttgatgata ataatacata tctggggtta attccctgtt
     3541 ctaccttaac caaatatttc gcaaatatta catcattaca acaaccgggt gctattatag
     3601 tgttagaaat gcatacaaaa gactatacat tacaaaaaat aacacaaata attgaagaaa
     3661 atagtgcaaa aatcataagt ttatatacca ccattattcc cgattctact aaaattgagc
     3721 ttacaataaa attaaacaca catgatatag gcgaaataat tgcagaactt aatcgctttg
     3781 attatactat aaaaacatac tttgaaggat atagcaaatt gcatgaatta tataaaaaca
     3841 gaattgataa tcttactcac tatttaaaca tataataatg aatactgttg cattatatgg
     3901 agcttcaata tcagaagaaa tttacacagc tgtttacaat ataattcaaa aactacagca
     3961 a
//
LOCUS       DAOTOW010000113         3949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18393_length_3949_cov_3.070621, whole genome shotgun sequence.
ACCESSION   DAOTOW010000113 DAOTOW010000000
VERSION     DAOTOW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18393_length_3949_cov_3.070621"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="P5243_02860"
     CDS             <1..912
                     /locus_tag="P5243_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18420.1"
                     /translation="FLFIACVACSKTSEIMNISDIVPNDSYQLPEIAVHGIVTSELKY
                     QEIQITQPQAYNTAHPLFYDDSLFYDYHGISGVEIIIKIQNKEYEFIESLYHPSYPDY
                     KKRKGLYVSKDKMKGLINTEHILQIEYKNKTYYAKDFMKPVQAFNSYSEVLSNFIIDS
                     TTIKYFNTNLGATDCFFVELSYTDTIHIDSSLLELYDFSIWNYYFSIQLPPALLEESK
                     KISSQTAYNDRPDRLYTIKKYSVSDAYQKYTLSVLSETLWNKSSFRSMPSNVATNLSK
                     GAVGFFAVCDVYSKQTTFKKLFENTKY"
     gene            1262..2383
                     /locus_tag="P5243_02865"
     CDS             1262..2383
                     /locus_tag="P5243_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18421.1"
                     /translation="MIPRKLQSILEKVLFSNKAIIILGARQVGKTTLLKNVIINKQDI
                     IWLNADEIAVRTMFENASSERFKTLFGNAKIIIIDEAQRIESIGIKLKLFTDTMPHLQ
                     VIATGSSAFELANKINEPLTGRKWEYTMYPISFAEMVDHHGLITELQSLETRLIYGYY
                     PEVINSKGNEKKILHQLTDSYLYKDILEWERIKKPEKLLKLLQALAFQIGNEVSLNEL
                     SQIVEIDKNTVEKYITLLEQIHVIFKLQSLSRNARNELKASRKIYFYDNGIRNALITN
                     FSLPEQRQDIGALWENFIISERKKYIQYNELWVQHWFWRTTAQQEIDFIEEADGVITG
                     YEFKWNPKTHVSFSKTFTNSYPNSNTKVIHRDNFFEFLL"
     gene            complement(2375..3895)
                     /locus_tag="P5243_02870"
     CDS             complement(2375..3895)
                     /locus_tag="P5243_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18422.1"
                     /translation="MNLKFLIFNFFVLFYCYSYSQELVTYYYSDSTISAQGYMRNDKP
                     DGYWKNYYPSGKLKSEGKRTNFELDSIWKFYTEQGFIQSEISYFEGKKNGEFKEYIVE
                     NDTVFLSSITMYVNDNKQGKAYYYLKGKLQKIIPYENNEMQGQSFEFDNTGEISAIVV
                     YKNNELISKQSINRTNAQGKKQGTWMEFYPNGNIKTEANYSNGKLQGLYKLFNSRQEL
                     LQVGNYDADSLVYSSQIMDDFEEPFEKREWYNDSILKYKGTYKDIVPIGVHRLYNEKG
                     EIVSGLLYNAQGTIVGKGITLENGKKQGAWIFYYPDGTKESEGSFSNNEKTGLWKFYY
                     PSGTIKQEGSYARNKISGVWKWYSETGVLRKEEQYAGGKKNGLSIQYNEQKQIIVQGE
                     YIDDKQHGVWIYKTGDIITKAVYEYGDKTKTWESHYISNGKLQFKGSYYMGQPKGKHV
                     YYYNNGKLEHEEIYKNGKPAKAWSYYSYQGELLYVVYYKKGVEDKIVTVSNKHGLQ"
BASE COUNT         1403 a          597 c          481 g         1468 t
ORIGIN      
        1 tttttattta tagcttgtgt ggcatgtagt aaaacatctg aaattatgaa tatatctgat
       61 attgttccta atgattctta ccaacttcca gaaatagcag ttcatggcat tgtaacttcc
      121 gaattaaaat atcaagaaat tcaaatcaca caaccacaag catacaacac cgcacatcca
      181 ttattttatg atgattcttt attttatgat tatcatggaa tatcaggagt tgaaataatt
      241 ataaaaatac aaaacaaaga atatgaattt attgaatcat tatatcatcc tagttatcct
      301 gattataaaa aaagaaaagg tttatatgtt tcaaaagata aaatgaaagg actgataaat
      361 acagaacata ttttgcaaat tgaatataaa aataaaacgt attacgccaa agattttatg
      421 aaacctgtac aagcatttaa ttcgtattca gaagtgttgt caaattttat tattgatagt
      481 actacaatta aatactttaa tacaaattta ggtgctactg attgcttttt tgttgagctg
      541 tcatacactg acacaattca tatagattcc tctttattag aattatatga cttttctatt
      601 tggaactact atttttctat tcaacttcct cctgcattat tggaagaatc aaaaaaaatc
      661 tcatcacaaa cagcatataa cgatagacct gatagattgt acacaataaa aaaatattct
      721 gtttcagatg catatcaaaa atatacactt tcggtattaa gtgaaacttt atggaataaa
      781 agctcatttc gttccatgcc aagtaatgtg gccacaaatc ttagtaaagg tgctgttggt
      841 ttttttgcag tatgtgatgt gtatagtaaa caaactactt ttaaaaaatt atttgaaaat
      901 accaaatact aaattttatt tataagagac attattaaaa tctaaattaa ttaataatac
      961 agccttattt atatagtact atatgaaaaa taattttttt atggaattac attcatgttt
     1021 ttatttattt gcattataat actccctatg tataagacag tttaattatc ttttttttag
     1081 atataatttt cattattatt catttttcaa ttttagtgta ttccactgca ctaaaaacaa
     1141 ataaaatatt gagaaaatat atgattttgg tgaatcaatt cactaaaaat tataatattt
     1201 ggtgaattga ttcactaaat gtagtatatt tgtttgtaat aaatataaaa ttaaaatatc
     1261 tatgatacct cgcaaactac agtcaatatt ggaaaaagta ctattttcga ataaagcaat
     1321 tattattctt ggagctcgac aagttggaaa aacaacatta ctaaaaaatg tcataataaa
     1381 caaacaagat ataatttggt taaatgctga tgaaatagcg gtacgtacaa tgtttgaaaa
     1441 tgcttcatcg gaaagattta aaactttgtt cggaaatgca aaaattataa ttattgatga
     1501 agctcaacga attgaatcaa taggtattaa attaaaatta tttaccgata caatgcctca
     1561 tttacaagtt atagcaacag ggagttcagc atttgaactt gcaaataaaa taaacgaacc
     1621 attaaccgga agaaaatggg aatatactat gtatcctata tcttttgctg aaatggttga
     1681 tcatcatggt ttaattacag aattacaaag tttagaaact cgcttaatat atgggtatta
     1741 tcctgaggtt ataaatagca aaggaaatga aaaaaaaata ttacatcaat taactgatag
     1801 ttatttatac aaagatattt tggaatggga acgtataaaa aaacctgaaa aattacttaa
     1861 attattacaa gctcttgcat ttcaaattgg aaatgaagtt tcattaaatg aattatcaca
     1921 aatcgtagaa attgataaaa ataccgttga aaaatacata acccttcttg aacaaattca
     1981 tgtcattttt aaattacaat cattaagtag aaatgcacga aatgaattaa aagcaagcag
     2041 aaaaatatat ttctatgaca atggaattag aaatgctctg attacgaatt tttcattacc
     2101 ggaacaacga caagatatcg gagcattatg ggaaaatttt ataataagtg aacgcaaaaa
     2161 atatattcaa tataatgaat tatgggtaca acattggttt tggcgaacta ctgctcaaca
     2221 agaaattgat tttattgaag aggctgatgg agttattaca ggatatgaat ttaaatggaa
     2281 tccaaaaaca catgtttcat tttcaaaaac atttactaat tcttatccta attcaaatac
     2341 aaaagttatt cacagagata atttctttga atttttactg taacccatgt ttattcgaca
     2401 ctgttacaat cttatcttcc acaccttttt tataatacac cacatacagt aattctcctt
     2461 gatacgaata gtacgaccag gctttggcag gtttgccgtt tttataaatt tcttcgtgtt
     2521 ctaatttacc gttgttataa taatacacat gttttccttt gggttgaccc atgtaatatg
     2581 aacccttgaa ttgcagttta ccattggaaa tatagtgcga ttcccatgtt ttggttttat
     2641 cgccatattc atatacagct ttggttataa tatcaccggt tttgtaaatc catactccat
     2701 gttgtttatc atcaatatat tcaccttgta caattatttg tttttgctca ttatattgaa
     2761 tacttaatcc atttttttta ccacctgcat attgttcttc ttttcgtaaa acaccggttt
     2821 ctgaatacca tttccataca cctgatattt tatttcttgc ataacttcct tcttgtttta
     2881 tagttcccga tggataataa aatttccaca agcctgtttt ttcattgttc gaaaacgatc
     2941 cttctgattc tttggtacca tcgggataat aaaaaatcca tgctccttgt tttttaccat
     3001 tttctaaagt aattcctttt cctactattg ttccttgtgc attgtacaac aatcccgata
     3061 caatttctcc tttttcatta tataaccgat gaactccaat aggtacaata tctttgtatg
     3121 ttcccttata ttttaatatt gaatcattat accattctcg cttttcaaat ggttcttcga
     3181 aatcatccat tatttgactt gaataaacta aactatcggc atcataattt cccacttgta
     3241 ataattcttg tcgtgaatta aataatttat acaaaccttg taactttccg tttgaataat
     3301 ttgcttctgt ttttatattt ccattcggat aaaattccat ccatgttcct tgttttttac
     3361 cttgagcatt agttctatta attgattgtt ttgaaattag ctcattattt ttatatacaa
     3421 caattgcaga aatttcgcct gtattatcaa attcaaaact ttgaccttgc atttcattat
     3481 tttcatatgg aattattttt tgaagttttc cttttaaata ataatacgct ttaccttgct
     3541 tattatcatt tacatacata gtaatagaac ttaaaaatac agtatcattt tctacaatat
     3601 attctttaaa ttctccattt ttttttcctt caaaatatga tatttctgat tgaataaatc
     3661 cttgttccgt ataaaatttc caaatactat ctaattcaaa atttgtacgt tttccttctg
     3721 attttaattt gcctgatgga taataatttt tccaatatcc atcaggttta tcattacgca
     3781 tatatccttg cgccgaaata gtactatcgg aataatagta cgtcactaat tcttgcgaat
     3841 acgaatagca ataaaataat acaaaaaaat taaaaattaa aaattttaaa ttcataattg
     3901 tttagtttca ccacgttgtt aatggaaaaa cgtctccccc ttcctttgc
//
LOCUS       DAOTOW010000114         3940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18442_length_3940_cov_3.444530, whole genome shotgun sequence.
ACCESSION   DAOTOW010000114 DAOTOW010000000
VERSION     DAOTOW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18442_length_3940_cov_3.444530"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5243_02875"
     CDS             <1..220
                     /locus_tag="P5243_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HRS18423.1"
                     /translation="NFIVKIDASNGKVIERINLSNILDKAYMHENIDVLNGIAWHAKN
                     KSLFVTGKRWPILFEIQIVDSTSASKTL"
     gene            231..1355
                     /gene="hisB"
                     /locus_tag="P5243_02880"
     CDS             231..1355
                     /gene="hisB"
                     /locus_tag="P5243_02880"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HRS18424.1"
                     /translation="MEQKKRVLFIDRDGSIIVEPKHDFQVDSFEKLEFLPRAISSLQT
                     IAEQSTYILAMVTNQDGLGTDSFPEDTFWPVHNKMLTILANEGVTFSDIVIDRTFPHE
                     NKPTRKPGTALLQHYLTDTYDLANSFVIGDRITDIELAKNLGAKAIFINHDNITIPKE
                     LQQYCALITQSWTEITTFLLFPPRYAEVIRKTNETDIQIKLLLDGAGKSNISTGLGFF
                     DHMLEQLARHSNCDITIQVKGDLHIDEHHTIEDTALALGEAFLKALGNKQQIERYGFL
                     LPMDESLAQVALDFSGRPWLVWDVTFTREKVGDLPTEMIYHFFKSFSDAARCNLNIQV
                     TGDNEHHKIEAIFKALAKAIKMAVSRNSKNIGLPSTKGVL"
     gene            1426..2883
                     /gene="rpoN"
                     /locus_tag="P5243_02885"
     CDS             1426..2883
                     /gene="rpoN"
                     /locus_tag="P5243_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRS18425.1"
                     /translation="MLNQKLQQKLQQKLSPQQIQVIKMLEIPTMMLEQRIKEELEENP
                     ALEFEETPDDIRDQTGENDEIQEDDNTNDEEFSIDDYFNEDEYSNYKYKSDNYSSDTD
                     NKEIPFSVGNTFHENLIQQIGLQDLDEREQQIAEFIIGNIDEDGYLRRELENIVDDIM
                     FAQNIETSVSELKKILSVIQTFDPPGVGATTLQECLLLQITRKQRTAETITATAILRD
                     FYNEFTKKHYEKIQQKLDIESDDLKDAIDEILKLNPKPGSSYSDPLNKTAGQVIIPDF
                     ILEEQNGELTISLHSRNVPELKISKTYADMLTAYKANSQVSKSQKDALTFVKQKLDSA
                     KWFIDAIKQRHQTLLSTMHAIFEYQKDFFTTGDETKLRPMILKDIADKTMLDVSTISR
                     VANSKYIQTPYGIYALKYFFSEGLQNEAGEEVSSREIKTILQEAIENENKSKPYTDEE
                     LVDVLKEKGYIIARRTIAKYREQLGLPVGRLRKEL"
     gene            3022..>3940
                     /locus_tag="P5243_02890"
     CDS             3022..>3940
                     /locus_tag="P5243_02890"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRS18426.1"
                     /translation="MSVKQKIKKISLWIGGIIAGLLIITSVLSYIYRDKIITFVTQEL
                     SSQLQGEIRIGKIDISFLSSFPNVSIQLHNIIAKSTPGFNTKEFNEYNTDTAFTAQKL
                     SFVFNVIDFINERYIVQQIKVKGAYINLFIDSKGKHNMNFVKETNDTVQTNYFIELSK
                     ISIFKTHTHIHSAADKLYAHDYFDFAHISGKFLENGFSLFVDTEFKNHIISIDNQSFF
                     PNLDIDTEISIAKTENLIQILKASIATPLITIQTDGSVRLQNKSTETKLSYNFTIEDI
                     EEFMSIMPQSIATMAKEHNLEGSIKANGTI"
BASE COUNT         1555 a          627 c          576 g         1182 t
ORIGIN      
        1 caactttata gttaaaattg atgcttctaa tggtaaagta attgagcgaa taaatctttc
       61 aaatattctt gacaaagcat atatgcatga aaacattgat gtactcaacg gtatagcatg
      121 gcatgcaaaa aacaaatcgc tttttgtaac cggtaaacgt tggccgattt tgtttgaaat
      181 tcaaattgtc gacagcactt ctgcatcaaa aacactgtaa attcaaaaat atggaacaaa
      241 aaaaacgagt attgtttatt gatcgcgatg gtagtataat tgtagaacca aaacatgatt
      301 ttcaagtaga ttcatttgaa aaattagaat ttttgccacg tgctatttct tcattacaaa
      361 ctatagcaga acaatctacc tatatactcg caatggttac aaatcaagac ggattgggaa
      421 cagattcgtt tcctgaagat acgttttggc ctgttcacaa taaaatgctt actatattag
      481 caaacgaagg tgtaactttt tctgatattg taatagaccg cacattccca cacgaaaaca
      541 aacctactcg taaaccagga acagctctgc ttcaacacta tcttaccgat acgtatgatt
      601 tagcaaattc atttgtaata ggagaccgaa ttaccgatat tgaattagca aaaaatttag
      661 gagcaaaagc tatctttata aatcatgaca acattacaat tcctaaagaa ttacaacaat
      721 attgtgcatt aataacacaa tcgtggacag aaatcacaac atttttattg tttccgccac
      781 gctatgccga agttattcga aaaaccaatg aaaccgatat acaaattaaa ctattgcttg
      841 atggggcagg aaaatcaaac atttctacag gattaggttt ttttgaccac atgcttgaac
      901 aacttgctcg tcattccaat tgtgacatta ccattcaggt aaaaggagat ttacatatag
      961 atgaacacca taccattgaa gatacagcat tagctttagg cgaagcattt ttaaaagctt
     1021 taggcaacaa acaacaaatt gaacggtatg gttttttatt acctatggat gaatcattag
     1081 ctcaagttgc attagatttt tcaggacgac cttggctagt ttgggatgta acttttacac
     1141 gtgaaaaagt tggcgactta ccaaccgaaa tgatatatca tttttttaaa tcattttctg
     1201 atgctgcgcg ctgtaatcta aatatacaag taacaggcga taatgaacat cacaaaattg
     1261 aagcaatttt taaagcgttg gcaaaagcaa taaaaatggc tgtttccaga aattcgaaaa
     1321 acattggttt accaagtaca aaaggtgttt tataattttt ttactatcat atatagcaaa
     1381 tcttatatca aaaagtacta tatttgaata tcaaaaaaat atagtatgtt aaaccaaaaa
     1441 ttacaacaaa aactacaaca aaaattatcg ccccagcaaa ttcaagttat taaaatgctt
     1501 gaaataccta ctatgatgct ggaacaacga atcaaagaag aattagaaga aaaccctgct
     1561 ctagaatttg aagaaacacc tgacgatatt cgtgatcaaa ccggcgaaaa cgatgaaatt
     1621 caagaagatg acaatacaaa tgatgaagaa ttttcaatag acgattactt taacgaagac
     1681 gaatattcaa actataaata taaaagtgat aattattcct ctgataccga taataaagaa
     1741 atacctttct cggtaggaaa tacgtttcat gaaaatttaa tacaacaaat aggattacaa
     1801 gacttagatg aacgtgaaca acaaatagct gaattcataa ttggaaatat agatgaagac
     1861 ggatacttgc gtcgtgaact cgaaaatata gttgacgata tcatgtttgc tcaaaatatt
     1921 gaaacttctg tatcagaact aaaaaaaatt ctttcagtta ttcaaacgtt tgatcctccc
     1981 ggagttggag caactacatt acaagaatgc ttattattac aaattactcg taaacaacgt
     2041 actgccgaaa caattactgc aacagcaata cttagagatt tttataatga atttacaaaa
     2101 aaacattacg aaaaaattca acaaaaatta gatatagaat ctgacgattt aaaagatgct
     2161 attgatgaaa tcttaaaatt aaatcctaaa cccggcagtt cctacagcga cccactcaat
     2221 aaaactgcag gtcaagttat aattcctgat tttatattag aagaacaaaa tggtgaactt
     2281 acaataagtt tacattcacg aaatgtacct gaactcaaaa taagtaaaac atatgccgat
     2341 atgctaactg cttacaaagc aaattctcaa gttagcaaat cacaaaaaga tgcccttacg
     2401 tttgtaaagc aaaaattaga ttcagctaaa tggtttattg atgccataaa acaaagacat
     2461 caaaccttat taagcactat gcatgcaatt tttgaatatc aaaaagattt ttttactacc
     2521 ggcgacgaaa ccaaacttcg gcctatgatt ttaaaagaca ttgcagacaa aacaatgctt
     2581 gacgtttcta caatttccag agtggcaaat agtaaatata ttcaaactcc ctatggaatt
     2641 tatgccctta agtatttctt ttcagaagga ttacaaaacg aagcaggcga agaagtttct
     2701 tcaagagaaa ttaaaacaat actccaagaa gctatagaaa atgaaaataa atcaaaacca
     2761 tataccgatg aagaacttgt tgatgttctt aaagaaaaag gatatataat agctcgtcgt
     2821 acaattgcaa aatacagaga gcaattagga ttacctgttg gaagattacg caaagaattg
     2881 taatcctttg atttctgaaa attctaaaaa acaagaatta tttcacacac aacagatata
     2941 aaataaattc gaggaaaaac cgtatgttac aaagtaattt ttatattttt gaatacaatc
     3001 aaaactcgga atgtatagaa tatgtctgtt aaacaaaaaa taaaaaaaat tagtctttgg
     3061 attggaggta taattgcagg attactcata attacctctg tattgtctta tatatatcgc
     3121 gataaaatta taacatttgt aactcaagaa ttgagcagtc agttacaagg agaaataagg
     3181 attggaaaaa ttgacatttc atttttaagc agttttccta atgtttccat tcaattacac
     3241 aatataatag ctaaatcaac tcccggtttt aatactaaag aatttaatga gtataacaca
     3301 gatactgctt ttactgcaca aaaactatca tttgttttta atgttattga ttttattaat
     3361 gaacgatata ttgttcaaca aattaaagta aaaggagcat atattaacct tttcattgat
     3421 tcaaaaggca aacacaatat gaattttgta aaagaaacaa acgatacggt acaaactaac
     3481 tactttatag agttatctaa aatcagtatt tttaaaactc atactcacat tcatagtgct
     3541 gccgacaaat tgtatgctca cgattatttt gattttgctc atatttcggg taaatttttg
     3601 gaaaatggat tttcattatt tgtagatact gaatttaaaa atcatattat atctattgac
     3661 aatcaatctt tcttcccgaa tttagatata gacacggaaa tttcaattgc aaaaacagaa
     3721 aatcttattc aaattctaaa agcaagtatt gcaacacctt taattactat acaaacagat
     3781 ggttcagttc gtttacaaaa caaatcaacc gaaacaaaat tgtcatataa ctttactatt
     3841 gaggatattg aagaatttat gagtattatg ccacaatcaa tagctaccat ggcaaaagag
     3901 cataatttag aaggaagtat taaagcaaat ggaactataa
//
LOCUS       DAOTOW010000115         3939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18450_length_3939_cov_2.358136, whole genome shotgun sequence.
ACCESSION   DAOTOW010000115 DAOTOW010000000
VERSION     DAOTOW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18450_length_3939_cov_2.358136"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1683
                     /locus_tag="P5243_02895"
     CDS             124..1683
                     /locus_tag="P5243_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HRS18427.1"
                     /translation="MKKIFIILIIINVIIISAIVYLTKNQKHIDALQLLEEQYSQHHE
                     PSVDHTQFSVLQQKFKSPQDVTAACLSCHTERGKEVMKTRHWKWQNEEYIEGRGLTNL
                     GKKNILNNFCIGIGGNEQSCNRCHAGFGWADTTFDFHNQHNIDCMVCHDNSGEYFKQS
                     GGAGNPDPSVNLNKVATHVGKPKKTNCGSCHFYGGGGNNVKHGDLEQAQMNCTKDVDV
                     HMAKNGINMECVDCHVTKKHVIAGKMYSVSSTNTNRLSCTQCHGNTPHELSVLNEHTI
                     KVACQTCHIPTYAKANATKMTWDWSTAGKLKNGEPYHEEDSMGNHSYLSEKGTFVWAK
                     NVKPEYVWFNGTADHYMFGDKVDTTKPIAMNTLRGSYSDPAAQIIPVKVHRAKQLFDP
                     QYGNIIQPKLFAKEKGQGALWKDFDFNAASATGMKYIGQPYSGTYTFVHTESFWPLNH
                     QVAPKEQSLSCVECHSRNGRLANITDFYLPGRDRHAGVELFGIISIIVSLVGIAIHAT
                     LRIISHRKELK"
     gene            1694..2317
                     /locus_tag="P5243_02900"
     CDS             1694..2317
                     /locus_tag="P5243_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRS18428.1"
                     /translation="MKKKVYIYKAYERFWHWTQAFLVFFLAFTGFEIHGTFEFFGFRN
                     AVIYHNAAAVSFMILIVFTIFWHFTTGEWKQYVPTTKNIKAQILYYAVGMFKNAPHPV
                     HKTELSKLNPLQRIVYFLLKVVFIPLMVISGLLYMFYRYPHNGNIVSINTEGLTTIAV
                     LHTLGAFIMIAFVVVHLYLITTGTTIFTNLKAMLTGYEEIEEEESKN"
     gene            2323..>2532
                     /locus_tag="P5243_02905"
     CDS             2323..>2532
                     /locus_tag="P5243_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18429.1"
                     /translation="MKTQKQEYINPYIGGFLLGLVLLASIYFSGRGLGASGAIKSFVV
                     SAVDFVAPQHTQNSEFYQQYTGGKPS"
     assembly_gap    2533..2542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2543..2847
                     /locus_tag="P5243_02910"
     CDS             <2543..2847
                     /locus_tag="P5243_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRS18430.1"
                     /translation="SWLVFEVLGVFIGAFISGIVSNRMTLKIDKNPVVSSVKRLFFAT
                     IGGLFFGLGAQFARGCTSGAALSGMGVLATGGFITMLAIFGTGFLFAFLFKKQWVY"
     gene            2866..>3939
                     /locus_tag="P5243_02915"
     CDS             2866..>3939
                     /locus_tag="P5243_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRS18431.1"
                     /translation="MGPLAGQGIISPEFDYIIALLIGIAFGFVLEQSGFSSAKRLAGL
                     FYGREFVVLRVFFTAAITTVIGVILMEYFGMLDMNLVYVQPTFTWSAIVGGVIMGIGF
                     LFGGFCPGTSLCAVSIGKIDAMFFFGGLFLGVLLFAEMYPMFEPLYHAQNLGGIFVYD
                     SLGMSRGVFAALLIIMAVMAFVVTYFIEQYILKKPKKFNYEFITSKPYHVAGIVIALI
                     FAGIVAVTPTRKDSIMKYVEHTISQKTMSFQKVSSVELTYLLLNPDNQTLYIDIRNPQ
                     DFEKLALPGSVNIPFESIMNREWEDFLGDATKKKIIISNDTLFAQKAIVIMTRLGYKH
                     VYMLQDNFQEFQATVLQFDAAHSK"
BASE COUNT         1332 a          531 c          738 g         1328 t
ORIGIN      
        1 taataatatt catattaaaa cattgacaaa tttaaaaaat actaatataa tgtgacaaaa
       61 gttactttgc aatattaaaa aatatgtgta tctttacatg aatacttaag tttatgttat
      121 tttatgaaaa agatttttat aattctcatt ataataaatg ttataattat atcagctatt
      181 gtatatctta caaaaaatca aaaacacatt gatgctttgc aattgttaga agagcaatat
      241 agtcagcatc atgaaccctc tgtagatcat acacaatttt cggtgttgca acaaaaattt
      301 aaatcgcctc aagacgttac ggcagcttgt ttgagttgtc ataccgaacg aggtaaggag
      361 gtaatgaaaa cgagacattg gaaatggcaa aatgaagaat atatagaagg tagaggatta
      421 acaaatctag ggaaaaagaa tatacttaat aatttttgta taggaatagg tggtaatgaa
      481 caatcatgta atcgatgtca tgccggtttt ggttgggcag atactacatt tgattttcat
      541 aatcaacata atattgattg tatggtttgt catgataatt caggtgagta ttttaaacaa
      601 tcaggtggtg ccggaaatcc agatccaagt gtgaatctta acaaagttgc aacacatgtt
      661 ggaaaaccta aaaaaaccaa ctgcggatct tgtcattttt acggaggagg aggaaacaat
      721 gtaaaacatg gcgacttaga acaagctcaa atgaattgta caaaagatgt agatgtgcac
      781 atggcaaaaa atggaattaa tatggaatgt gttgattgtc atgtgactaa aaaacatgta
      841 attgcaggaa aaatgtattc tgtatcgtct acaaatacaa acagacttag ttgtacacaa
      901 tgccatggca atactccgca tgaactgagt gttttaaatg aacatactat aaaagttgct
      961 tgtcaaacgt gtcacattcc tacatatgcc aaagccaatg caactaaaat gacatgggat
     1021 tggagtactg caggtaaatt aaaaaatgga gaaccttatc atgaagaaga ttctatggga
     1081 aatcattcgt atttatcaga aaaaggtact tttgtgtggg caaaaaatgt aaaacccgaa
     1141 tatgtatggt ttaatggcac tgccgatcat tatatgtttg gcgacaaagt agataccact
     1201 aaacccattg ctatgaatac attacgaggt tcgtacagtg atccggcagc tcaaataatt
     1261 cctgtaaaag tacatagagc caaacaatta tttgaccctc aatatggaaa tataattcaa
     1321 ccaaaattat ttgctaaaga aaagggacaa ggagcactat ggaaagattt tgattttaat
     1381 gctgcaagtg ctaccggtat gaaatatata ggtcaaccat acagcggaac atatacattt
     1441 gtgcatactg aaagtttttg gccgctcaat caccaagtgg cacccaaaga acaatcatta
     1501 tcttgtgttg aatgtcattc tcgaaatggt cgattagcaa atattactga tttttattta
     1561 cccggtcgag ataggcatgc aggagttgaa ttatttggca taatatctat tattgtatca
     1621 ttagttggaa tagctattca tgctactttg cgaattatat cacacagaaa agaattaaaa
     1681 taaaagtatt tctatgaaga aaaaagtata tatttataaa gcttatgaac gtttttggca
     1741 ctggactcaa gcttttctcg tgtttttttt agcatttaca ggttttgaaa ttcatggaac
     1801 ttttgaattt tttggtttta gaaatgcagt aatatatcat aatgcagcag cagtttcttt
     1861 tatgattctt atagtgttta ctatattttg gcattttaca acaggtgaat ggaaacaata
     1921 tgtgccaact accaaaaata ttaaagctca aattctttat tatgctgttg gtatgtttaa
     1981 gaatgcacct catcctgttc ataaaacaga attaagtaaa ttaaatccat tacaacgaat
     2041 tgtttatttt ttgttgaaag tggtatttat tccgcttatg gtaatatccg gcttattata
     2101 tatgttttat agatatccgc acaatggaaa tattgtttct ataaatacag aaggtttaac
     2161 tactatagcg gtactgcata cactgggggc ttttataatg atagcgtttg ttgtagttca
     2221 tttatattta attactacag gaacaaccat atttacaaat ctaaaagcta tgcttaccgg
     2281 atatgaagaa atagaagaag aagaaagtaa aaattaagaa ctatgaaaac acaaaaacaa
     2341 gagtatataa atccgtatat tggaggcttt ttattgggat tagtattatt ggcttcaata
     2401 tatttttcgg gacgtgggct tggtgcaagt ggtgcaatta aaagttttgt tgtgtcggct
     2461 gttgattttg ttgcaccgca gcatactcaa aattcagaat tctatcaaca atacaccggc
     2521 ggtaaaccaa gtnnnnnnnn nnaatcatgg ttagtgtttg aagttcttgg tgtttttata
     2581 ggtgcattta tttcaggaat agtatcaaat agaatgacct taaaaataga taaaaaccct
     2641 gttgtaagtt cggttaaacg tttatttttc gcaacaatag gaggattatt ttttggactt
     2701 ggagctcaat ttgctcgggg gtgtacaagt ggagcagctc tcagtggtat gggggtttta
     2761 gctacaggtg gttttataac aatgcttgca atatttggaa caggattttt gtttgcgttt
     2821 ttgtttaaaa aacaatgggt ttattaattt gaaaatacta aaactatggg accattagca
     2881 ggacaaggaa ttataagccc cgaatttgat tatattattg cattacttat aggaattgca
     2941 ttcggttttg tattagaaca atcaggattt tcatcggcta aacgtttggc aggtttattc
     3001 tacggaagag aatttgtagt gttacgagta ttttttactg cagctattac tactgtaatt
     3061 ggtgtaattc ttatggagta ttttggtatg ttggatatga atttggtgta tgttcaacct
     3121 acatttacgt ggtcggctat agttggggga gtaattatgg ggattggatt ccttttcgga
     3181 ggtttttgcc ctggaacttc attgtgtgca gtgtcaattg gcaaaattga tgcaatgttc
     3241 ttttttggag gtttattttt aggtgttttg ctttttgccg aaatgtatcc gatgtttgaa
     3301 cctttgtatc atgctcaaaa tttaggtgga atatttgtgt atgattcatt gggaatgagt
     3361 agaggtgtat ttgctgcatt attaattatt atggctgtta tggcttttgt ggttacatat
     3421 tttatagaac aatacatact caaaaaacca aaaaaattca attacgagtt tattacatca
     3481 aaaccctatc atgttgcggg tattgtaatt gctttaattt ttgcaggtat agttgctgtt
     3541 actcctactc gtaaagattc aattatgaaa tatgtagaac atacaatttc tcaaaaaaca
     3601 atgtcatttc aaaaagtttc ttctgtagaa ttaacatacc tattattaaa tccagataat
     3661 caaacattat atattgatat acgaaatcca caagattttg agaaattagc tttgcccgga
     3721 tctgtaaata ttccttttga atctattatg aatagagagt gggaagactt tctaggcgat
     3781 gcaaccaaaa agaaaattat tataagtaat gatacattat ttgctcaaaa ggcaatagta
     3841 ataatgacac ggttaggcta taagcatgta tatatgttgc aagataattt tcaagaattt
     3901 caggctacag ttttgcaatt tgatgctgct cattcaaag
//
LOCUS       DAOTOW010000116         3922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18540_length_3922_cov_3.925524, whole genome shotgun sequence.
ACCESSION   DAOTOW010000116 DAOTOW010000000
VERSION     DAOTOW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18540_length_3922_cov_3.925524"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="P5243_02920"
     CDS             <1..1025
                     /locus_tag="P5243_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRS18432.1"
                     /translation="LFSLQAEYYDIIHILLIKVSKSIFRYIFFYIGISMASYNSIAQQ
                     SPRSTLSTYHQMTYNPSIVGNTKYGNIQTVVREQYMNFSDNSGPSSVWFNASVPLSKL
                     QSGVGITINQLQQGFEKRLDFKANYAYHIDLGSGKFGAGLSIGGRNIGWDIKNPIYPD
                     GQNDAFVDTKIANKENFVNLLVGFGVYYKVNNIFTSLAITEMNKPKLQTEGSKVEYYN
                     RTYWLSAGYELKTSNPMWIIKPGVLVKTTITDTQLNFDMITEYNNFIIAGLTYTSSHD
                     ISPIFGVQFNDGSKFDGLRAIISYDIVTSKIGAQSSGNMEFTVGYNFNLSVEKENKTY
                     KSVRFL"
     gene            1076..2476
                     /locus_tag="P5243_02925"
     CDS             1076..2476
                     /locus_tag="P5243_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS18433.1"
                     /translation="MKKLLFFSSVILLLSSCSEYQTGELTGSKIRPAWEAEVPFGMLY
                     IPLGSLNLGPSDQDVPWAQTAQNRTVSLQAFWMDETEITNNEYRQFVEWVKDSLAYVK
                     LRDDGGKEDLYVATNTYGEDIPEEDQYIDWSKPIDWDETENREYLADMYLQGDERFYK
                     KKELDVRQLNYTFYWMDFKQAARKNKIGGKENRAYNYKTGKYEGEVLKADGSRVAIKD
                     RSSYIIKEEINVYPDTLCWVSDFTYSYNDPMSAMYFWHPAYDHYPLVGVSWKQAVAFS
                     VWRTQMKNGYLRSMGRVIVNDYRLPTETEWEYAARGGLASSPYPWGGNYTRNYLGCFI
                     ANFKPMRGNYTDDGGIHTVIVAHYEPNEWGLYDMAGNVSEWTQTAFDESSYSFTHDNN
                     TQNYYSALDDDPIVLKRKVVRGGSWKDIAYYLQNGTRAYEYQDSAKSYIGFRCVQTYL
                     GRNFGSDGQQGDSQVY"
     gene            2533..3510
                     /gene="gldL"
                     /locus_tag="P5243_02930"
     CDS             2533..3510
                     /gene="gldL"
                     /locus_tag="P5243_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRS18434.1"
                     /translation="MSIAELVESDGYKKFMAKVYGFGASIVLVGALFKIQHYPGASIM
                     LIIGLLTEALIFFFSAFEPLHEELDWTLVYPELAGLDEIEENVNIKKGGSQQDGTALQ
                     KFDELLEKGKLGPELFEKLGANLDALNNNVVSMTNISNASVATETYVSNINKAAGSAD
                     SLAGAFASSASKISQTVESIAESSKQTAESIINSGAKSAELISQSAKQTASIIETSGK
                     SYQQLLENLNANFSSIGESSKVQAVQQEALTKNLSALNAVYELQLKNSSTNLEATEKI
                     THGINSIMSDLQSTAEDVTKYKTEISKLSQNLAALNTVYGNMLSAMSIK"
     gene            3552..>3922
                     /locus_tag="P5243_02935"
     CDS             3552..>3922
                     /locus_tag="P5243_02935"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18435.1"
                     /translation="MGHGKETPRQKMIGLMYLFLTCMLALNVSKDILDAFININNRLN
                     ETNANFISKNQIAYDAIFKAYSANPVKVKAVYDYSQLLKRKSDIMKKLDSQIKAYVEN
                     FSTQEREMRRMVSVNELFEEQ"
BASE COUNT         1444 a          488 c          719 g         1271 t
ORIGIN      
        1 ttttattttc tttacaagct gaatattatg atattattca tattttatta attaaagtga
       61 gtaagagtat ttttagatat attttttttt atatcggcat tagtatggcg agttataata
      121 gtatagcaca acaatctcct aggagtacat taagtacgta tcatcagatg acgtataatc
      181 cttctattgt tggtaatact aaatatggta atattcaaac agtggttcga gaacagtaca
      241 tgaatttttc agataattcc ggtccgtctt cagtttggtt taatgcgtca gtgcctttat
      301 caaaattaca aagtggagta ggaattacaa taaatcagtt acaacaagga tttgaaaaaa
      361 gattagattt caaggcaaat tatgcctatc atatagattt aggtagtggt aaatttggtg
      421 caggtcttag tattggaggt cgaaatatag gatgggatat taagaatcct atctatcctg
      481 atggacaaaa tgatgcattt gttgatacaa aaattgcaaa taaagaaaat tttgtaaacc
      541 ttttagtagg ttttggcgta tactacaaag taaataacat ttttacatct ttagcgatta
      601 ctgaaatgaa taaaccaaaa ttacaaacag aaggttctaa agttgaatat tataatagga
      661 catattggtt gtctgcaggg tatgagttaa aaacatcaaa cccaatgtgg ataataaagc
      721 ctggagtttt ggttaaaaca acaataactg acacacaatt aaattttgat atgattactg
      781 aatataataa ttttataatt gctggtttaa catatacaag tagtcacgat atatcaccaa
      841 tttttggagt tcagtttaat gacggatcaa aatttgatgg tttacgagca attatttcat
      901 atgatatagt aacatctaaa ataggtgctc aatcatctgg aaatatggag tttacggtag
      961 ggtataattt taatttaagt gtggaaaaag aaaataaaac atataaaagt gtaagatttt
     1021 tgtagaattt taaaatttat tattaacttg cagattattt taaaaaaaag tgcatatgaa
     1081 aaaactattg ttctttagtt cggttattct cttgttaagc agttgctccg agtatcaaac
     1141 aggagagctt accgggtcga aaattcgtcc ggcatgggaa gctgaggttc cttttggaat
     1201 gttatatatt ccattaggta gtttgaatct tggtccgagt gatcaagatg ttccatgggc
     1261 tcaaacagct caaaacagaa cagtttcatt acaagctttt tggatggatg aaacagaaat
     1321 tacaaataac gagtatcgtc aatttgttga gtgggtaaaa gactctttag cttacgttaa
     1381 attgcgggat gacggaggta aagaagattt atacgtagca acaaatacat acggtgagga
     1441 tattcctgaa gaagatcagt atatagattg gtcaaaacca attgattggg atgaaactga
     1501 gaatcgagaa tatcttgcag atatgtattt acaaggagat gaacgttttt ataagaaaaa
     1561 agaacttgat gttcgtcaat taaattatac attttattgg atggatttta aacaagctgc
     1621 tcgtaaaaat aaaataggcg gaaaagaaaa tcgtgcttat aattataaaa ccggaaaata
     1681 tgaaggtgaa gtattaaaag ctgatggatc tcgagttgct attaaagata gatcatcata
     1741 tattataaaa gaagaaataa atgtgtatcc tgatacatta tgttgggtaa gtgactttac
     1801 atattcatat aacgatccga tgagtgctat gtatttctgg catccggctt atgatcacta
     1861 tccattagtt ggtgtatcat ggaaacaagc tgttgctttt agtgtttgga gaactcaaat
     1921 gaaaaatgga tatttacgta gtatgggaag agttattgta aatgattatc gattaccaac
     1981 cgaaacagaa tgggaatatg ctgctcgtgg aggtttagct tcttcaccat atccatgggg
     2041 aggtaactat acacgtaact acttaggttg ttttatagct aactttaaac ctatgcgtgg
     2101 taattataca gatgacggtg gtattcatac tgtaattgtt gctcattatg aaccaaatga
     2161 atggggatta tatgatatgg caggaaacgt ttcagaatgg acacaaacag catttgatga
     2221 atcatcatat tcttttacac atgataataa tactcaaaat tattattcag cattagatga
     2281 tgatccaatt gttttaaaac gcaaagttgt tcgtggtggt tcatggaaag atatagctta
     2341 ttatttgcaa aatggtacac gtgcatatga atatcaagat tctgcaaaaa gttatattgg
     2401 atttagatgt gtacaaacat atttagggcg taactttgga tctgatggac aacaaggtga
     2461 ttcacaagta tattaaaaag aatgtttaat aaaataaaat atatagtata aatagtttaa
     2521 ttgttttaaa atatgagtat tgcagaatta gtagaaagcg atggttataa aaaatttatg
     2581 gccaaagtgt atggatttgg tgcatcaatt gtgcttgttg gagcactttt taaaattcaa
     2641 cactatccgg gtgcaagtat aatgcttatt attggtcttt taacagaggc gttaatattt
     2701 ttcttctctg ctttcgagcc attacacgaa gaattagatt ggactttggt atatccggaa
     2761 ttagccggtc ttgatgaaat tgaagagaat gtaaatatta aaaaaggagg ttctcaacaa
     2821 gatggaactg ctttacaaaa atttgatgaa ttattagaaa aaggcaagtt aggtcctgaa
     2881 ttatttgaaa aactaggtgc aaatttggat gcgttaaata acaatgtcgt tagtatgaca
     2941 aatatatcaa atgcgtctgt agcaacagaa acttatgttt ctaacataaa taaagctgct
     3001 ggttctgctg atagtttggc aggagcattt gcaagttcag cttctaaaat atcacaaaca
     3061 gttgaatcta ttgcagaatc ttcaaaacaa actgcagaat caataataaa ttcaggagca
     3121 aaatctgcag aattaatttc tcaatcagct aaacaaacgg catcaattat tgaaacatca
     3181 ggaaaaagct atcaacaatt attggaaaat ttaaatgcaa acttctcatc aattggcgaa
     3241 agcagtaaag ttcaagcagt acagcaagaa gcattaacca aaaatttatc agcattgaat
     3301 gctgtatatg aattacaact aaaaaattca agtactaatt tagaagctac tgaaaaaatt
     3361 actcatggaa ttaattctat tatgagtgat ttacaatcaa cagcagaaga tgttacaaaa
     3421 tacaaaacag agatatctaa attaagtcaa aatttagctg ctcttaatac ggtgtatggt
     3481 aatatgttat ctgcaatgag tattaaataa ttattatttt attacattat tttttaaatt
     3541 agaaatagac gatgggacac ggaaaagaaa ccccaaggca gaagatgata ggtttaatgt
     3601 atttattcct aacttgtatg cttgcgttaa acgtttctaa agatattctt gatgctttta
     3661 ttaatatcaa taatagattg aatgaaacaa atgcaaattt tattagtaaa aatcaaattg
     3721 catatgatgc catttttaaa gcgtatagtg ctaacccagt aaaggttaaa gctgtttatg
     3781 attattctca attattaaaa agaaaatcag acataatgaa aaaactagat agtcaaataa
     3841 aagcttatgt tgaaaatttt tcaacacaag aacgcgaaat gcgccgtatg gtttcagtaa
     3901 atgaattatt tgaagaacaa ag
//
LOCUS       DAOTOW010000117         3912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18601_length_3912_cov_3.413015, whole genome shotgun sequence.
ACCESSION   DAOTOW010000117 DAOTOW010000000
VERSION     DAOTOW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18601_length_3912_cov_3.413015"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="P5243_02940"
     CDS             <1..414
                     /locus_tag="P5243_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18436.1"
                     /translation="ENDWIYKKVVVDAGDSENLRPGQIVTARRLRDENSQLKRKDLKL
                     VQVRDAQPATASQILQGITRAALQTQSFISAASFQETTKVLNEAAINGKVDELEGLKE
                     NVIVGHKIPAGTGLREYENIIVGSMEDFEKIKNHK"
     gene            506..820
                     /locus_tag="P5243_02945"
     CDS             506..820
                     /locus_tag="P5243_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRS18437.1"
                     /translation="MEENKQQLNIELSEEVAQGIYSNLAIITHSNSEFVVDFVRLMPG
                     IPKASVKSRIILTPEHAKKLLFALQDNIAKFEAMHGKIKITEGPGGGAMPLNFGPNTA
                     QA"
     gene            822..2003
                     /locus_tag="P5243_02950"
     CDS             822..2003
                     /locus_tag="P5243_02950"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRS18438.1"
                     /translation="MRHYYFLFFFICIVACKQQKVISDYNKPISDIETYFLITPTSDS
                     ITNIIYTISASDLYTQSSKNSKSYIYFEVENYDENKNQLIQSYTSPTKIIYVNNSSDF
                     IQGSFALPFYIPQNSSIRIRMLNTYNTLLRSYTYYIPQEKESFEILQNNTRNRWFKPY
                     VYVDTCVFINIVKNKEYLFYTFEPPYMNALNKPIPYRLPIIHDSVVVPFTKIGNYTFY
                     SDSLLQDTIFSIPAVSKKFPKNNSACIMLTALQLINPLLSLDSLLNTRVGCKIELDKQ
                     WLSIADNNEMKAKEAINSFYSRIEKANKLYTITKQGMYSDRGICLILLGEPQKISYNV
                     NSEIWYYSIDTTIQEKKFIFDKKNIGIQSEYYLKNKNEKKIFIDFAQLNWQRGNVFTL
                     Q"
     gene            2008..2745
                     /gene="rlmB"
                     /locus_tag="P5243_02955"
     CDS             2008..2745
                     /gene="rlmB"
                     /locus_tag="P5243_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRS18439.1"
                     /translation="MEKNNLIYGIHAVLETLREGKPIDQILIKKQSDNELIREIIYNA
                     RKQGVTVKSVPYEKIQRITQKNHQGVLAFLSPIEFADIHDIVPHIFEEGKVPLIVLLD
                     SITDVRNFGAIARTCECLGVHAIVIPQKGAAQINADAVKTSAGALHKIPICKTKSLTH
                     ACNFLQDSGIQIIAASEKYSKMCKEINFTLPTALVMGAEEDGISKPILDLCDEHIGIP
                     MFGNIGSLNVSVATGICLYEAVRQRSM"
     gene            2745..3380
                     /locus_tag="P5243_02960"
     CDS             2745..3380
                     /locus_tag="P5243_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRS18440.1"
                     /translation="MNQSLKHIEAIIFDFGGVVLNLDEQQTYTQMSNLLGISLEDLQN
                     QVLRSKEFFNLECGTITPNKFIEFIHSKSKNNITDSQIIKAWNAMLLDLPQQHVTLLL
                     ELKKHFRTFLLSNTNAIHEDCFVTNIKNQGYTITLQDMFETVWYSHVLGLRKPNKDIF
                     IEVAKRADLNPTNTLFIDDRIDNIQTAQELGFKTHHITPQHGIIDYFAGYL"
     gene            complement(3429..>3912)
                     /locus_tag="P5243_02965"
     CDS             complement(3429..>3912)
                     /locus_tag="P5243_02965"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRS18441.1"
                     /translation="IEPNFEGNGVIDAVLHVDSNNWEHLCLRAGGALLPASHETVDAH
                     QHFIHQEGQPVFKIAVNGMSGAARDILSRNNITGDDIAYLIPHQANKRIIDSTGHFLG
                     LTPEKVCVNIEKYGNTTAATIPLCMYEFAHKFKKGDNIILAAFGAGYSWGALFLKWAY
                     "
BASE COUNT         1500 a          595 c          553 g         1264 t
ORIGIN      
        1 gaaaacgatt ggatttacaa aaaagtagtt gtagatgcag gtgattctga aaatttacgt
       61 ccgggacaaa tagttacagc acgtagatta cgcgatgaaa attcacaatt aaaacgcaaa
      121 gaccttaaac ttgttcaagt tcgtgatgca caacctgcaa cagcaagcca aatactgcaa
      181 ggtattactc gcgctgcatt acaaactcaa agctttattt cagcagcatc gttccaagaa
      241 actacaaaag ttctaaatga agccgctatt aatggtaaag ttgatgaact agaaggctta
      301 aaagaaaatg taattgtagg tcataaaata cctgcaggaa caggattacg tgaatatgaa
      361 aatattattg taggttcaat ggaagatttt gaaaaaataa aaaatcataa ataactactg
      421 taatattatt aaaataaaaa agtcatattg taatttttca atgtgacttt tttttataca
      481 tttactttta ttaaaaaatt gtattatgga agaaaacaaa caacaactta atattgaact
      541 atctgaagaa gtagctcaag gtatttattc caatcttgct attataaccc attcaaattc
      601 tgaatttgtg gttgattttg ttaggctaat gccgggaatt ccaaaagcaa gtgttaaatc
      661 aagaattatc ttaacaccag aacatgcaaa aaaactatta tttgctttac aagacaatat
      721 agcaaaattt gaagctatgc atggtaaaat aaaaattacg gaaggtccag gtgggggtgc
      781 tatgccatta aattttggac caaatactgc acaagcataa tatgcgtcac tattattttc
      841 tatttttttt tatttgtatt gtagcttgta aacaacaaaa agtaatatct gattacaata
      901 aaccaatttc agatatagaa acttattttt taattactcc tacttcagat tctataacaa
      961 atattatata cactatttct gcttctgatt tatatacaca atcaagtaaa aattcaaaat
     1021 catatatata ctttgaagta gaaaattacg atgaaaataa aaatcagctc attcaatcat
     1081 atacaagccc aacaaaaatt atatatgtaa ataattcaag tgattttata caaggttcct
     1141 ttgcgttacc tttttatatt ccccaaaatt caagtattcg aattcgcatg ctcaatactt
     1201 acaatacgtt gcttcgtagt tatacttatt atattcctca agaaaaagaa tcttttgaaa
     1261 ttttacaaaa caatactcga aatagatggt ttaaacctta tgtatatgtt gacacatgtg
     1321 tttttataaa tattgttaaa aacaaagaat atttatttta tacatttgaa ccaccatata
     1381 tgaatgcact aaataaacca ataccctata ggttgccaat aatacatgat tcagtagttg
     1441 ttccttttac aaaaatcgga aattatacat tttatagtga ttctttactt caagacacaa
     1501 tatttagcat tcctgctgta tctaaaaaat tccctaaaaa taatagtgct tgtattatgt
     1561 taactgcact ccaacttatt aatcctttat tatcgttaga ttctttactc aatacacgtg
     1621 taggttgtaa aattgaactt gataaacaat ggttatctat agctgataat aatgaaatga
     1681 aagcaaaaga agcaattaat agcttctata gcagaattga aaaagctaat aaattatata
     1741 caattacaaa acaaggaatg tattcggata ggggtatttg tttaatactt ttaggagaac
     1801 ctcaaaaaat atcatacaat gtaaattcag aaatttggta ttattcaatc gatacaacca
     1861 ttcaagaaaa aaaatttatt tttgacaaaa aaaatattgg aatacaatca gaatattatt
     1921 taaaaaataa aaacgaaaaa aaaatattta ttgactttgc acaactaaat tggcaacgcg
     1981 ggaacgtatt tacattacaa taactctatg gaaaaaaata atcttatata tggtattcat
     2041 gctgttttag aaactctccg agaaggtaaa ccaatagatc aaatccttat aaaaaaacaa
     2101 tctgataatg aattaatacg tgaaataata tataatgctc gtaaacaagg tgtaacggtt
     2161 aaaagtgtac catatgaaaa aattcaacgc attacacaaa aaaatcatca aggagtgttg
     2221 gcatttttat ctccaataga atttgcagat attcatgata tagttccaca catatttgaa
     2281 gaaggaaagg ttccacttat agtactatta gattctatta cagatgtaag aaattttgga
     2341 gctattgcaa gaacgtgcga atgtttaggt gtacatgcca tagtaattcc tcaaaaagga
     2401 gcagctcaaa taaatgctga tgccgtaaaa acatcagcag gagcacttca caaaattcct
     2461 atatgcaaaa ctaaatcctt gactcatgcc tgcaatttct tacaagattc aggtattcaa
     2521 attattgcag cctctgaaaa atacagtaag atgtgtaaag aaataaattt cacattacct
     2581 actgcattag taatgggggc tgaagaagat ggtatttcaa aacctatatt ggatttgtgt
     2641 gacgaacata ttggaattcc aatgtttgga aatattggtt cacttaatgt atcagttgca
     2701 accggtattt gtttatatga agctgttaga caaagaagta tgtaatgaac caatcattaa
     2761 aacatataga agccataatt tttgattttg gaggagttgt tctcaattta gatgaacaac
     2821 aaacatatac tcaaatgagt aatcttctcg ggatttcttt agaagattta caaaatcaag
     2881 tattacgaag taaggaattt tttaatttgg aatgtggcac aattacaccg aataagttca
     2941 ttgaatttat tcatagtaaa tcaaaaaata acataaccga tagtcaaatt ataaaagctt
     3001 ggaatgcaat gttattggat ttaccacagc aacatgtaac attactttta gaactcaaaa
     3061 aacattttag aactttttta ttaagcaata caaatgctat tcatgaagat tgttttgtaa
     3121 caaatattaa aaatcaagga tatactatta ctttacaaga tatgtttgaa accgtttggt
     3181 attcacatgt tttaggatta cgcaaaccca ataaagatat atttattgag gtagctaaaa
     3241 gagccgattt aaatcctaca aatactttat ttatagatga tagaatagat aatattcaaa
     3301 cagctcaaga attaggcttc aaaactcacc acataactcc tcaacatggt ataattgatt
     3361 attttgctgg ttacttataa aaaaaaagcc gattttaaaa atcggctttt ttatattcaa
     3421 taatttactt aatatgccca ttttaaaaac aaagctcccc aactatatcc tgcaccaaat
     3481 gcagcaagta ttatattatc accttttttg aatttatgtg caaattcata catacaaagt
     3541 ggtatagtag cggctgtagt atttccgtac ttttcaatat ttacacaaac cttttcaggt
     3601 gttaatccca ggaaatgccc tgtagaatca ataattcttt tatttgcttg atgagggatt
     3661 aagtatgcaa tatcatcacc tgttatatta tttctgctta gaatatctcg tgcagctccg
     3721 gacataccat ttacagcaat tttaaaaaca ggttgacctt cttgatgaat aaaatgttga
     3781 tgtgcatcaa cagtttcatg acttgccggg agcaaagcac ctcctgcacg taaacacaaa
     3841 tgctcccaat tattactatc tacgtgcaaa acagcatcaa ttacaccatt accttcaaaa
     3901 ttaggttcaa tc
//
LOCUS       DAOTOW010000118         3906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18636_length_3906_cov_3.248767, whole genome shotgun sequence.
ACCESSION   DAOTOW010000118 DAOTOW010000000
VERSION     DAOTOW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18636_length_3906_cov_3.248767"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="P5243_02970"
     CDS             <1..450
                     /locus_tag="P5243_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18442.1"
                     /translation="IGVTKIYKHMKTKTSFARFYMLLAQLPGAEKEELIWQYSNMLTT
                     SLQEFYTANPTSYEHMLQDMEQQCSNNMQEVKFMRSAVLKRMQQCGVDTTKWSEVNKF
                     LAYPQIAGKMLFEMSVEEMQQLIPKLEKIIAKNIVKRKENQRLMQCN"
     gene            450..707
                     /locus_tag="P5243_02975"
     CDS             450..707
                     /locus_tag="P5243_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18443.1"
                     /translation="MRNNEKSDLRIQKKNDKEQLCELRKKFAQIETQIDEAVRKYDIS
                     NWKQLWAQKNQLRIKIDALKERMSDKASNYRGVTCYSTLQV"
     gene            704..889
                     /locus_tag="P5243_02980"
     CDS             704..889
                     /locus_tag="P5243_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18444.1"
                     /translation="MTVKERKPISITTFKKSKIVECAYCKGTGINDDGVVCEKCDGTG
                     ELERVAEGNIKMYKLKI"
     gene            896..1540
                     /locus_tag="P5243_02985"
     CDS             896..1540
                     /locus_tag="P5243_02985"
                     /inference="COORDINATES: protein motif:HMM:NF022807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HRS18445.1"
                     /translation="MNLENLSTDELEALEAQLAQKKKEKKEKERQEREAFKQLQHEYV
                     ENNFCKLQDVEKNLSTIKSELFENVESILAMKKEIYGIDDKQQSHSISNKACTKTIIV
                     GHNIVDGWDADTAAAGVERVNQWLTKKLNDNNADLINIIRDLLKPNQEGILKANRVLE
                     LRNQAEKLGDQELISAVAVIQEAYRPKKTTTFIKAKFRNEHGQDVWLNLSMSNA"
     gene            1601..1912
                     /locus_tag="P5243_02990"
     CDS             1601..1912
                     /locus_tag="P5243_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18446.1"
                     /translation="MTTIQIVHNTILMLATQITFIGCRTWNVKAIAKNNMPQVILSGV
                     IVNLSWLVSMGLGGVSMNAIINDFQWEHVPIVLACIIGGVVGTYIGMYDKTKRNGDIR
                     I"
     gene            1893..2087
                     /locus_tag="P5243_02995"
     CDS             1893..2087
                     /locus_tag="P5243_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18447.1"
                     /translation="MATSEFKAIVDALEHRYSQNITAIERLELTRLLSFYYRKIERSK
                     QQQTVVIIQSKANTYEIFEN"
     gene            2068..2286
                     /locus_tag="P5243_03000"
     CDS             2068..2286
                     /locus_tag="P5243_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18448.1"
                     /translation="MKYLKINPGQTIEMRADTDNVYFAVTEAILLAQKYLIEINLFVG
                     EYMMGIDKDTVISDMMSEYSYWLKKQMK"
     gene            2549..3091
                     /locus_tag="P5243_03005"
     CDS             2549..3091
                     /locus_tag="P5243_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18449.1"
                     /translation="MTVKKNELQTMSLTVTEGLTVTILPSLSHQFLMTTKEVANGYGT
                     SKYAIQQAFHRNSSELIEGKHFVTALTYCQRDSKLPHNAILFTKRGIVRLGFFIKSER
                     AKLFRDWAEDLIIAIDNHDNQKNLFGEIVTTQRQLPTKRKHNRLTHERLLNIMADVCK
                     IENSELRLAISTKLMQGGVQ"
     gene            3305..3514
                     /locus_tag="P5243_03010"
     CDS             3305..3514
                     /locus_tag="P5243_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18450.1"
                     /translation="MITFEKNKMVIELPTDGCPVEEWLQTHTDMVELLQAEEVQMHQR
                     RWHYLELLKHLVPDWNTAQKMIHSK"
     gene            3653..3874
                     /locus_tag="P5243_03015"
     CDS             3653..3874
                     /locus_tag="P5243_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18451.1"
                     /translation="MDTILGFFTNINGVFVLSSPERSVEVQEIYNDIMSLDYEPLNAD
                     KKNMALDLSNFRKSFKNSVSSYKSEKIHG"
BASE COUNT         1520 a          637 c          698 g         1051 t
ORIGIN      
        1 attggggtga caaaaatcta taaacatatg aaaacaaaaa catcatttgc acggttttac
       61 atgctattgg cacaattgcc gggagccgaa aaagaggagc ttatatggca atactcaaac
      121 atgcttacaa catcgctaca ggaattttat accgccaatc ctacatcgta tgaacatatg
      181 ctccaagata tggagcagca atgctctaac aatatgcaag aagtaaaatt catgcgtagt
      241 gctgttctta aacgcatgca acaatgtggt gttgatacta caaagtggag cgaagtaaat
      301 aaatttctcg catatccgca aatagcaggg aaaatgctgt ttgagatgag cgtagaagag
      361 atgcaacaat tgataccaaa acttgaaaaa attatagcta aaaacatagt aaaacgtaaa
      421 gaaaatcaac gattaatgca atgcaactaa tgagaaataa tgaaaaatct gacttacgta
      481 ttcagaaaaa aaacgataaa gaacagttgt gtgagcttcg taaaaagttc gcacaaattg
      541 aaactcaaat agatgaagca gttagaaaat acgatataag caattggaag caattgtggg
      601 ctcaaaaaaa ccagctacgt attaaaatag atgctctcaa agagcgaatg agtgataaag
      661 catctaacta tagaggtgta acatgttatt caacactaca ggtatgacag taaaagagcg
      721 aaaaccaata agcataacca cgtttaaaaa atcaaaaata gtggagtgcg catactgtaa
      781 aggaaccgga ataaatgatg atggtgtagt atgtgaaaaa tgcgacggaa ccggagaatt
      841 ggagcgagtt gcagaaggta atataaaaat gtataaactt aaaatttaaa aatcaatgaa
      901 tttagaaaac ctatcaaccg acgaattaga agctttagaa gctcagttgg cacaaaagaa
      961 aaaagagaaa aaagaaaaag aacgtcaaga acgtgaagcg tttaagcagt tacaacacga
     1021 atatgttgag aataattttt gcaagttgca agatgttgaa aaaaatctat ctaccataaa
     1081 atctgaatta tttgaaaatg tagagtctat acttgcaatg aaaaaagaaa tttatggtat
     1141 agatgacaaa cagcaatcgc acagcataag caataaggcg tgtacaaaaa ctataattgt
     1201 tggtcacaac atagtagacg gttgggatgc tgatacagca gctgctggtg ttgaacgagt
     1261 aaaccagtgg ctaactaaaa aactaaacga caacaatgcc gacttaatta acattattcg
     1321 cgacttgctt aaaccaaacc aagaaggtat tctaaaagcg aatagagtac tcgagcttcg
     1381 taatcaagca gaaaaattag gagatcagga attaatatct gccgtagctg taattcaaga
     1441 agcatatcgt cccaaaaaaa caacaacatt tataaaggcg aagtttcgaa acgaacacgg
     1501 acaagatgtg tggctcaacc tctcaatgag caacgcataa taaatcaccc acgccccacg
     1561 gctacgagca gtcgtggggt tacctaaaac catttaaaca atgacaacaa ttcaaatcgt
     1621 acacaatacc atacttatgc ttgcaaccca aataacattt ataggttgca gaacatggaa
     1681 tgtaaaagca atagcaaaaa acaacatgcc tcaagtgatt ttaagtggcg ttatcgttaa
     1741 tcttagttgg cttgtaagca tggggcttgg tggtgtgagt atgaatgcta taatcaatga
     1801 ttttcagtgg gagcatgttc ctattgtttt agcttgtatt ataggtggtg ttgttggtac
     1861 atatatagga atgtacgata aaactaaacg caatggcgac atcagaattt aaagctatag
     1921 ttgatgctct tgagcataga tatagccaaa acattacagc aatagaacgg ctcgaactta
     1981 cacgattact ctctttctat taccgaaaaa tagaacgttc aaaacagcaa caaacagtag
     2041 taataattca atcaaaagca aacacctatg aaatatttga aaattaatcc cggacaaact
     2101 atagaaatgc gagcagatac tgacaatgta tattttgcag taactgaagc tatactcttg
     2161 gcacaaaaat accttataga aataaattta tttgttggtg aatatatgat gggtatagat
     2221 aaagatactg taatatctga tatgatgtct gaatattcat actggttaaa aaaacaaatg
     2281 aaataatgaa acatttattt tcaaaattac cacttgtata tacaaaaata attataaact
     2341 attgactttc tcattttcat tcgtatattt gtcccgactg ttatttctat ataactcttg
     2401 ggcaaattaa tttgatttaa tttaaacaag ataaaggcaa tgcccattat ggtggatggt
     2461 acgaaagacc catcgactta aatgcccagc gtagaagtga cagtcacacc tacggtgggc
     2521 attgctgttt atcattattc ttaacaacat gactgtaaaa aagaatgaat tacagactat
     2581 gagtctgacc gtaaccgaag gattgacggt tacaatttta ccaagtttaa gccaccagtt
     2641 tttaatgaca accaaagaag ttgctaacgg ttatggaact tccaagtatg cgattcaaca
     2701 ggcatttcat cggaatagtt cagaattgat tgaaggaaag cattttgtaa ccgcattgac
     2761 atattgtcaa cgcgattcca aattgccaca taatgcaatt ctgtttacaa aaagaggcat
     2821 agttcgttta ggttttttta taaaaagcga acgagcaaaa ttgtttcgcg attgggctga
     2881 agatttaatt attgcaattg acaatcatga caatcaaaag aatctatttg gtgaaattgt
     2941 aacaacacaa cgccaattac caactaaacg taaacacaac cgccttacac acgagcgttt
     3001 gcttaatata atggcagatg tttgtaaaat tgaaaattct gaattacgcc ttgctatttc
     3061 aactaaactt atgcaaggag gtgtacaatg aatgtaatat ttgtaaacca caaaagcgat
     3121 ttgacggaac aaattggcct ttacgaagga acaaatttta aaacattccg tacacgcact
     3181 acgtgtaatt gctcacacaa aaacaataat aggaatgcag tactcgtact atcacctaca
     3241 ggctatgtaa acagctgcta tatacgttgc aaacagtgta aattaaccca aactatataa
     3301 aactatgata acatttgaaa aaaacaaaat ggtaatagag ctcccaacag atgggtgccc
     3361 tgtagaagaa tggctacaaa cacacaccga tatggtagaa ctactgcagg cagaagaagt
     3421 gcaaatgcac caacgccgct ggcactactt ggagctactc aagcatttgg tacccgactg
     3481 gaatactgca caaaaaatga ttcatagtaa ataatatata tttaaaaccc cttaaaaaca
     3541 aattaagggg cttttttttg catttaatgt aatttttata ctaaatttac gtacaaatag
     3601 tatgtgtgat tgtataaaaa atattagttt tgacaaatat ttacataaat ctatggatac
     3661 aatacttggt ttttttacaa atattaatgg cgtttttgtt ttgtcttcac ccgaaagaag
     3721 tgtagaagtg caagagattt ataatgatat aatgtcttta gattacgaac cgttgaatgc
     3781 agataaaaaa aatatggcat tagacctttc taactttaga aaatcattta aaaattctgt
     3841 ttcttcatat aaatctgaaa agatacatgg ctaaacaaac tcacaatcaa aaaattgcta
     3901 aaagca
//
LOCUS       DAOTOW010000119         3893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18707_length_3893_cov_2.882491, whole genome shotgun sequence.
ACCESSION   DAOTOW010000119 DAOTOW010000000
VERSION     DAOTOW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18707_length_3893_cov_2.882491"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="P5243_03020"
     CDS             <1..318
                     /locus_tag="P5243_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18452.1"
                     /translation="RHAVTLSPIDFFACAQTLLSNQGILSIIIPCSEKQTFCQQALYT
                     NLHIIKEIEISPYYETPPNRIILQFSKTWKSVQRTHIAIRNKDKTYSKEYKELTEKFI
                     SIN"
     gene            432..881
                     /gene="umuD"
                     /locus_tag="P5243_03025"
     CDS             432..881
                     /gene="umuD"
                     /locus_tag="P5243_03025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HRS18453.1"
                     /translation="MNATRIHTSTRIDIYSANTESTLALPYVSEGISAGFPSPALDFT
                     DISIDLNKHLISHPSSTFYGRVKGNSMKDEGIFDGDLLVIDKSLEPSNRKIAVCYIDG
                     EFTVKKIKIEHDSCWLIPANNEYKPIQVTADNDFLIWGIVTYVIKSV"
     gene            881..2140
                     /locus_tag="P5243_03030"
     CDS             881..2140
                     /locus_tag="P5243_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRS18454.1"
                     /translation="MFALIDCNNFYASCERVFRPDLQHKPIVVLSNNDGCIIARSNEA
                     KALGLPMGAPCYQYKDLIEKHNVTVFSPNFALYGDMSQRVMTLLSEFSPDIEIYSIDE
                     AFLKFHGFDLFDLQSYGETIQKKVKKYTGIPISVGIAPTKALAKIANRIAKKFPKQTN
                     SCYYIHTEEQRIKALKWTHIGDVWGIGRKHSKRLQSIGVQNAYDFTLLEDCWVQKHMS
                     IIGLRLKHDLQGIPTLDIEEIQAKKNIATTRSFETNYTTFDQIKERVATFAISCSEKL
                     RKQQSCCSTLLVFIHTNGHRIDLPQYSKNCIIHLPYATNSAIEITKFAIIALQNIYKP
                     GYAYKKAGVIVMDVLPEHTKQYNLFEESNPKHPAIMQAMDTINNTYGQHTLKLGIQNQ
                     KKMWKMKQEKLSPKYTTNIHDIITIHC"
     gene            2151..2753
                     /locus_tag="P5243_03035"
     CDS             2151..2753
                     /locus_tag="P5243_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRS18455.1"
                     /translation="MCFYFHQSKSAVEIAQRYNIHNTTQLQISLGIYNGFLFPLITTI
                     HQQNSQPTLQQMQWGLIPDWAKNKEIQKNTLNARWETIHEKPSFKYYTQNRCIIPATG
                     FYEWQWLDPKGKHKQKYNIQCSHNELFSIAGIWNIWQYNNQPIYTCSIITIPANSLMA
                     TIHNTKKRMPFILDINQESDWLTGKQIIPKDIELTAHTCM"
     gene            2842..3267
                     /locus_tag="P5243_03040"
     CDS             2842..3267
                     /locus_tag="P5243_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRS18456.1"
                     /translation="MNIQDIQDQIIEEFSIYEDWLDKYQYIIDLSKDLPLIDDQYKKE
                     NYVIKGCQSQVWLHADFKNGKVIYTADSDAVITKGIISLLIRVLSNQTPEDILNCDLY
                     FIEKIGLKENLSPTRSNGLVAMIKQMKLYAMACNYKQNQ"
     gene            3271..3597
                     /locus_tag="P5243_03045"
     CDS             3271..3597
                     /locus_tag="P5243_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HRS18457.1"
                     /translation="MATNTEQTAKLEEEIVATLRTIYDPEIPVNIYDLGLIYEINMKE
                     LPLVKIEMTLTSPNCPMTDVIMYQIENRVKLIESIEEVQVSIVFDPPWDKEMISEEGK
                     LKLGLL"
     gene            3600..>3893
                     /locus_tag="P5243_03050"
     CDS             3600..>3893
                     /locus_tag="P5243_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS18458.1"
                     /translation="MQQKIHMVDLVGQYEARKSEFDAAISSVIHQAAFINGPEVDTFQ
                     KELEEYLQVKHVIPCANGTDALTIALWALDLPPGSEIITSDFTFIATAEAIARV"
BASE COUNT         1564 a          666 c          579 g         1084 t
ORIGIN      
        1 cgtcatgcag ttacattatc tcctattgat ttttttgcat gcgcacaaac attactttca
       61 aatcaaggca tattatctat tattattcca tgttctgaaa aacaaacatt ttgccaacaa
      121 gcactctata ctaatttaca tattattaaa gaaattgaaa tttctccata ttacgaaact
      181 cctcccaata gaattatatt acaattttca aaaacatgga aatcggttca acgaacacat
      241 attgctattc gaaacaaaga caaaacgtat agcaaagaat acaaagaact tacagaaaaa
      301 tttatttcaa tcaattgatt ttcaaactat taaaatattt tgttttttat taaacaccgt
      361 tacattttaa taaaaatcgc cgtatatttg aaacaaattt attctataca aacaacagta
      421 ttattggtac catgaatgca acgcgtatac acacttcaac acgtattgat atatattcgg
      481 ccaatactga aagtacatta gcgttgccat atgtatcaga gggaataagt gccggatttc
      541 cttctccggc attagatttt acagatattt ctatagattt aaataaacat ttgatttcac
      601 acccatcgtc aacattttat ggccgagtaa aaggaaattc aatgaaagac gaaggaattt
      661 ttgacggtga tttattagta atagacaaaa gtttggaacc aagcaacaga aaaattgccg
      721 tatgctatat tgatggtgaa tttaccgtaa aaaaaatcaa aatagaacat gatagctgtt
      781 ggcttattcc ggcaaacaat gagtacaaac ctatacaagt aactgccgat aatgactttt
      841 tgatttgggg aattgttacc tatgtaataa aatcagtata atgtttgctc ttattgactg
      901 taataatttt tatgcttcgt gcgaacgtgt atttcgtcct gatttacaac acaaacctat
      961 tgtagtatta tcaaacaacg acggttgcat aatagctcgc agtaatgaag caaaagcatt
     1021 aggattaccc atgggagcac cttgctatca atataaagac cttatagaaa aacataacgt
     1081 aacggttttt tcaccaaatt ttgcactata cggagacatg agtcaacgag taatgacttt
     1141 gctaagcgaa ttttctcccg atattgaaat atacagtata gacgaagcat ttttaaagtt
     1201 tcatggtttt gatttatttg acctacaatc atatggagaa accattcaaa aaaaagtaaa
     1261 aaaatatacc ggaattccca taagtgtagg tattgcccca accaaagctt tagctaaaat
     1321 tgcaaatcgt attgccaaaa aatttcctaa acaaaccaat agttgctact atatccatac
     1381 cgaagagcaa cgaataaaag cactcaaatg gacacacata ggagatgtgt ggggaattgg
     1441 cagaaaacac agcaaacggc tacaaagcat aggagtacaa aatgcgtatg attttacctt
     1501 attggaagat tgttgggtac aaaaacatat gtcaattata ggtcttagat taaaacatga
     1561 tttacaaggc attcctacat tagatataga agaaatacaa gcaaaaaaaa atatagcaac
     1621 cactcgctct ttcgaaacta attatacaac atttgaccaa atcaaagaac gagtagcaac
     1681 attcgcaata tcgtgcagcg aaaaattacg caaacaacaa tcttgctgct ctaccctatt
     1741 ggtttttatt cacaccaacg gccatcgcat agatttaccg caatacagca aaaattgcat
     1801 aatacattta ccctacgcca caaattcggc aatagaaatt acaaaatttg caattatagc
     1861 attacaaaac atatataaac ccggatatgc atacaaaaaa gcaggagtaa ttgtaatgga
     1921 cgtactaccg gaacacacta aacaatacaa tttatttgaa gaatcaaatc ccaaacatcc
     1981 tgcaataatg caagctatgg ataccataaa caatacatac ggacaacata ccctaaaact
     2041 tggaattcaa aatcagaaaa aaatgtggaa aatgaaacaa gaaaaattat cacccaaata
     2101 cacaactaat attcacgata ttattaccat acactgttaa acaaaatagt atgtgttttt
     2161 attttcatca atcgaaatca gcagtggaaa ttgctcaaag atacaatata cataatacga
     2221 cacagctaca aatatcgcta ggaatatata acggattttt gtttcctctc attacaacta
     2281 tacatcaaca aaactcacaa ccaacgttac aacaaatgca gtggggactc atacctgatt
     2341 gggcaaaaaa caaagaaata caaaaaaaca cattaaatgc ccgatgggaa accattcatg
     2401 agaaaccatc atttaaatat tacacacaaa atagatgtat aataccggca acaggatttt
     2461 acgaatggca atggcttgac cccaaaggaa aacacaaaca aaaatataat attcaatgct
     2521 cacacaatga attattcagt atagcaggaa tatggaatat atggcaatac aacaaccaac
     2581 ccatctacac gtgtagtatt attacaattc cggcaaattc acttatggct acaattcata
     2641 atacaaaaaa acgaatgcct tttattttag atataaacca agaatcagat tggcttaccg
     2701 gcaaacaaat tataccgaaa gatattgaat tgacagcaca tacatgcatg taataaaaaa
     2761 aactaataaa ttacgcattc tgaaatttgt gattgaataa aaaaaaatta cttttgttta
     2821 tatgtaaaat aaataaaaca gatgaacata caagatatac aagaccaaat aattgaagaa
     2881 ttcagcatat atgaagattg gttagataaa tatcaatata taatagattt aagtaaagat
     2941 ttaccattga ttgacgacca atataaaaaa gaaaactatg taataaaagg atgtcaatca
     3001 caagtatggt tacatgctga ttttaaaaac ggtaaagtaa tatatacggc agatagtgat
     3061 gcggtaatca caaaaggtat tatttcttta cttatacgag tattatcaaa ccaaacaccc
     3121 gaagacattc ttaattgcga cttatatttt atagaaaaaa tcggtttaaa agaaaactta
     3181 tcacctactc gttctaacgg tttggtggca atgataaaac aaatgaaatt gtacgctatg
     3241 gcgtgtaatt acaaacaaaa tcaataaatt atggcaacaa atacagaaca aacggcaaaa
     3301 ttggaagaag aaattgttgc aacacttcga accatttacg accccgaaat tccggtaaat
     3361 atatacgacc ttggattgat atatgaaatt aacatgaaag aattaccatt ggtaaaaatt
     3421 gagatgacac ttacatctcc taattgtcca atgaccgatg ttataatgta tcaaatagaa
     3481 aatcgggtga aattaattga atcaatagaa gaggtacaag tatcaatagt gtttgaccca
     3541 ccgtgggaca aagaaatgat aagcgaagaa ggaaaattaa aattaggact cttataagta
     3601 tgcaacaaaa aattcatatg gtagatttgg tgggtcaata tgaagcacgc aaatcggaat
     3661 ttgacgctgc catatcatct gttattcatc aagcagcatt tattaatgga cctgaagttg
     3721 atacttttca aaaagaatta gaagaatact tacaagtaaa acatgtaata ccatgcgcaa
     3781 atggcaccga tgctcttacc atagcattat gggctttaga cttacctccg ggtagtgaaa
     3841 taataacttc tgactttaca tttattgcaa cagcagaagc aatagcaaga gtt
//
LOCUS       DAOTOW010000120         3887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18759_length_3887_cov_2.932672, whole genome shotgun sequence.
ACCESSION   DAOTOW010000120 DAOTOW010000000
VERSION     DAOTOW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18759_length_3887_cov_2.932672"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..576)
                     /locus_tag="P5243_03055"
     CDS             complement(22..576)
                     /locus_tag="P5243_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18459.1"
                     /translation="MRLDLTIREKLVIFYITLGFIAVSMLGVYSFKSAREAILNRSFE
                     QLTSVREVKKARLQHFFHDRIKETELLASTPELFNYTDLDSTIINTSQLDSFLLKGNY
                     ISSIHIVQPNKKLIHSKKIKQISSENILLTETEKDTLFAIAKQITNNNKTELFDYKKI
                     YSEYSIFIASPIISNHYCPIKLQN"
     gene            complement(719..2539)
                     /locus_tag="P5243_03060"
     CDS             complement(719..2539)
                     /locus_tag="P5243_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS18460.1"
                     /translation="MRDFIKLIPYLRRYKKYIALNIILNLLGAIFSFGTIATIIPFLS
                     ILFNTNQIVTESIPFELTKESLQHNSNYALSIIIQEYGKPATLLFICGFLVLITFLKN
                     IFEYLSQYFNIPVVNGVPKDLYNATFEKILHLPVYYFTNERKGDIMARMSSDITEVRN
                     SMVNSFTGFIKNPLYILIYFIGILIISWQMTLIILILLPITAIITGRIGKNLKKALHK
                     AQHLQGDILVFIDETLSGLKVIKVFNGIGYMQDKFKRNNYEYYKTMNNANRKHALAHP
                     ITEFLIIITLSFAIFYGGSLVLKNDGSMSAEGFIAFLLAFTQIVSPAKAFTNSFYGIK
                     RGAASIERIEKILHAEIQIIDSPTAKSISTFKEKIEFNNVTFSYTPERTVLQNISITL
                     EKGKSVALVGQSGSGKTTIAHLLPRFYEVEQGDILIDNISIKDIKLNDLRSLIGIVTQ
                     DSILFNDTIFNNIAFGIENPDEAKVIEAAKLANAHDFIMETPQGYQTNIGDQGSKLSG
                     GQRQRISIARAIMKNPQILILDEATSALDTESERLVQNALEHLMKDRTSLIIAHRLST
                     IKNADEIIVLQDGAIVERGSHEELVAKNGAYKRLQSMQMV"
     gene            complement(2641..3573)
                     /locus_tag="P5243_03065"
     CDS             complement(2641..3573)
                     /locus_tag="P5243_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS18461.1"
                     /translation="MKILLTGGTGFIGSHVLIKLIELGHSVTVLARNIEKVPAIHTLP
                     NTTVIQAQITDFETISSVVKGHDVCVHVALNYNDTSAYHMLMSDTAPSIFLANECAKA
                     GVSHFIYTSSTATNDSIYASANPPILNNTNAHVLTSSQHAPHTYYGATKAATENFLLS
                     ITGTTGMRVSIIRPGYTFGNPAFEGAYTQSDQRFHTIVEAAKLNKDIEIIKNDGTQFI
                     WAPDLASIYTAVLQSNYNKKIYFGLSQNFTSWEYIALQAIQICNSTSKLIINDLGWSN
                     NPIMFDVSDIQRDFHLQFNSLPHIPTHLEYLFNR"
     gene            complement(3587..>3887)
                     /locus_tag="P5243_03070"
     CDS             complement(3587..>3887)
                     /locus_tag="P5243_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRS18462.1"
                     /translation="QIKITDSLDIEELELNLIDAGLENTEAHEDILYVYADYTDFGNM
                     SKALESCNVEVVKSELQRIPNTSQEFTEEQLIDIEKLLDKIEEDDDVQAVYTNIA"
BASE COUNT         1254 a          521 c          618 g         1494 t
ORIGIN      
        1 gataaatttt gttttattaa attaattttg tagttttatc ggacaataat gatttgaaat
       61 aataggagat gcgataaaaa tagaatattc ggagtatatt tttttgtaat caaataattc
      121 tgttttattg ttgtttgtaa tttgttttgc tatagcaaat agggtatctt tttcagtttc
      181 tgttaagaga atattttctg atgatatttg ttttattttt tttgaatgta ttaatttttt
      241 attgggttga acaatatgga tagatgagat gtaattgcct tttaataaaa aagaatcaag
      301 ttgagaagtg ttaattatgg ttgaatctaa atcagtataa ttaaacaatt ccggggttga
      361 ggctaataat tcagtttctt tgattctatc atgaaaaaaa tgttgaagtc tggctttttt
      421 aacttcacgt actgaagtta attgctcaaa tgaacgattt agaatagctt ctcgagcact
      481 tttaaatgaa taaacaccta acatgcttac agcaataaaa cctaatgtta tataaaatat
      541 aacaagtttt tcgcgtattg ttaaatctaa tctcattaaa gcaacgaatt gtaattgtat
      601 tgtttatatt agtttttaat tataagcaaa tataataaaa tagtatcaat atttaattta
      661 atatgttatt ttaattacaa tttataatct ttactggtgt tttatggtag atgttattct
      721 ataccatttg catgctttgt aatcgtttgt atgctccatt tttagcaact aattcttcgt
      781 gagaacctcg ttctacaatt gctccgtctt gtaatacaat aatttcatcg gcatttttaa
      841 ttgttgataa tctatgagca attataagtg atgtacggtc tttcataagg tgttctaatg
      901 cgttttgaac caatctttct gattctgtat caagagcaga agtagcttca tctaaaatta
      961 agatttgagg atttttcatg atagctcttg ctatacttat tcgttgtctt tgtcctccgg
     1021 ataatttact tccctgatct ccaatgtttg tttgatatcc ttgaggtgtt tccattataa
     1081 aatcatgagc attggctaat tttgcagctt ctataacttt agcttcatcg ggattttcaa
     1141 taccaaaagc aatgttgtta aaaatagtat cgttaaacaa aatagaatct tgagtaacaa
     1201 ttccgatgag gctacgtaag tcatttaatt ttatatcttt aatagatata ttatctatta
     1261 aaatatctcc ttgttctact tcgtaaaaac gaggtagtaa atgagctatt gttgttttac
     1321 cagaacccga ttgaccaact aatgcaacag attttccttt ttctaaagtt atagatatat
     1381 tttgtaatac agttcgttcc ggagtatatg aaaaagttac attattaaat tcaatttttt
     1441 ctttaaaagt agatatagat tttgctgtag gtgaatctat tatttgtatt tcggcatgta
     1501 gaattttttc aattcgttct atacttgcag ctccacgttt gataccataa aatgaatttg
     1561 taaatgcttt tgccggactt acaatttgtg taaatgccaa taaaaaggct ataaatcctt
     1621 ctgccgacat tgaaccatca ttttttaata caagtgaacc accataaaat attgcaaaag
     1681 aaagggttat gataattaaa aattcagtga tagggtgggc taatgcatgt tttctatttg
     1741 cattattcat tgttttgtaa tattcataat tgtttctttt gaatttatct tgcatatagc
     1801 caattccatt aaatacctta ataactttaa ggccggataa tgtttcgtca ataaatacta
     1861 agatatcacc ttgtaaatgt tgagctttgt gtaatgcttt ttttaaattt tttccaattc
     1921 taccggtaat tatagctgta attggcaata aaataagaat aattaaagtc atttgccagc
     1981 taataattaa aataccaata aagtatataa gaatatataa aggattttta atgaagcctg
     2041 taaatgaatt taccatactg ttacgaactt ctgtaatgtc tgatgacata cgagccataa
     2101 tatcaccttt gcgttcattg gtaaaatagt ataccggtaa atgtaatatt ttttcaaagg
     2161 ttgcattgta taagtcttta ggtacgccat ttacgacggg tatgttaaag tattgcgata
     2221 agtattcaaa tatattttta agaaatgtta tcagtaccaa aaaaccacaa ataaataaga
     2281 gtgttgccgg ctttccgtat tcttgaatta taatagataa cgcataattg ctgttatgtt
     2341 gcaatgattc ttttgttaat tcaaatggaa tagattctgt aactatttga ttggtattga
     2401 ataaaatact tagaaaagga ataatagtag ctattgttcc aaatgaaaaa attgcaccta
     2461 aaagatttag aataatgtta agtgctatat attttttgta gcgacgtaaa tatgggatta
     2521 attttataaa gtctctcatt gttttttttt acaaaagtat acatttttta cgttagattt
     2581 tgagaaaaat caatttattt gtagttgatt gtaatgtgct tgttaagagt taaataatat
     2641 ttaacgatta aacaaatact ctaaatgagt tgggatatga ggaagtgaat tgaattgtaa
     2701 atgaaagtcg cgttgtatat ctgaaacatc aaacattata ggattattgc tccatcccaa
     2761 atcattgatg attagttttg atgtagaatt gcatatttgt attgcttgca atgcaatata
     2821 ttcccatgat gtgaagtttt gagataatcc aaaatatatc tttttattgt agttgctttg
     2881 taatactgcc gtatatatag atgctaaatc cggagcccaa ataaattgtg tcccgtcgtt
     2941 ttttataatt tctatatctt tattgagttt tgcggcttca actatagtat gaaatcgttg
     3001 atcagattga gtatatgctc cttcaaatgc cggatttcca aaggtatatc ccgggcgaat
     3061 tattgaaact ctcatgcctg ttgttccggt tatgctaagc aaaaaattct ctgtggcagc
     3121 ttttgtggct ccgtaatatg tatgaggtgc atgttggctc gatgtgagaa catgagcatt
     3181 cgtattgttc aaaataggag gattagcact tgcatatatg ctgtcgttag ttgctgtaga
     3241 tgatgtatat ataaaatgag aaacgcctgc tttagcacat tcattagcta agaaaattga
     3301 gggagctgta tcgctcataa gcatatggta tgctgatgta tcattataat ttaatgctac
     3361 atgtacacac acatcgtgtc cttttactac agatgatatg gtttcaaaat cagtaatttg
     3421 agcttgtatt actgttgtat ttggaagagt atgaatagcg ggtacttttt caatattacg
     3481 agccaataca gttaccgaat gacctaattc aataagtttt ataaggacat gagagccaat
     3541 aaaaccggtt cctcctgtta ataatatttt catgcagaat aagacattat gctatatttg
     3601 tatacacagc ttgtacatca tcatcttctt caattttatc aagaagtttt tctatatcaa
     3661 ttagttgctc ttcagtgaac tcttgacttg tatttggtat tcgttgtaat tcagatttta
     3721 caacttctac attacatgat tctaaagctt ttgacatatt gccaaaatca gtgtaatcgg
     3781 catatacata caaaatatct tcgtgagctt ctgtattttc taatcctgca tcaattaaat
     3841 taagctctaa ttcttcaata tcaagtgaat ctgtaatttt tatttga
//
LOCUS       DAOTOW010000121         3877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_18818_length_3877_cov_4.198325, whole genome shotgun sequence.
ACCESSION   DAOTOW010000121 DAOTOW010000000
VERSION     DAOTOW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18818_length_3877_cov_4.198325"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /gene="hemA"
                     /locus_tag="P5243_03075"
     CDS             <1..1212
                     /gene="hemA"
                     /locus_tag="P5243_03075"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRS18463.1"
                     /translation="IREKFALSNSEIPTFAEELQKQANFSDIVVISTCNRTEIYFSQQ
                     KHDGFTASILLFNCLIAFKKIDFAAERFFYTYINDDAVSHLFKVCSGIDSMILGEDQI
                     IGQVKEAYIYCTELALTDAVLMRLFQKSFEAGKKVRTHTQLNTGVTSVSFAAIELCNK
                     YVTDYSDKTLLLIGTGETGTLALQGFVEKGIQNILISNRTPEHAEKLIHTYSAEFIPI
                     HNLLESLHKADIIITATNAGHTFITKQSIIDSLKKRQNIPQVYIDLSVPRNIEESIRD
                     YSLTTLICVDDIQHVIDTNKDKRAEQIEHAQPIIDELVQEFMEWLSFRALQPAIRAIN
                     FHLQKIYRDEFHGFIVDNSKDVKENIKDFAGYLTQQYSRELIKNLKEITDNGRKELYL
                     QVINDLFRLER"
     gene            1225..2145
                     /gene="hemC"
                     /locus_tag="P5243_03080"
     CDS             1225..2145
                     /gene="hemC"
                     /locus_tag="P5243_03080"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRS18464.1"
                     /translation="MKITIGTRGSKLALWQSNSVKQLLEQAFPHITIQLQIIQTTGDK
                     ILDVALSKIGDKNLFTKEIETAMLRHEVDIAVHSLKDLPTQLPEGLCVAAVLPRAECR
                     DALLSKTGKTIHELTPHDIIGTSSLRRKAQLLAFNPQLTIHDIRGNVDTRIHKMQSGQ
                     YDALIMAAAGLQRLHYSHEITHIIEPTIMLPAVSQGIIGIECRSNDTHIREILQTIHD
                     SKTWYMAAAERTFLEQMNGGCQLPLGCYTSISQNTITLQAAILSVDGSKKLAFSHSGD
                     LQQAQTVAKQLAQTFYNAGAHEILNTIQHD"
     gene            2138..2899
                     /locus_tag="P5243_03085"
     CDS             2138..2899
                     /locus_tag="P5243_03085"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRS18465.1"
                     /translation="MTNSVLHNTRILLTGAAETSQELAEILSLYGATVKASPMIEIKK
                     IEITDTILKLFGSLSQFQWVVFTSKYGVSTFFDSITPKHIPQHIQWACVGKKTAQKLT
                     DYGYIPHFICSKNNGKDFAQEFSNYINGQFVSVFFPTGNLTENMITLSVSPNISITKC
                     IIYNTVMPSTIDTNIFTQIEHKQFDYIIFTSPSGVTNCMKASNNLPIWNYCTIISIGP
                     STSKRLQKYGCTSFIQAEEYNSQGIISVLQQLNSN"
     gene            2901..3869
                     /gene="hemB"
                     /locus_tag="P5243_03090"
     CDS             2901..3869
                     /gene="hemB"
                     /locus_tag="P5243_03090"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRS18466.1"
                     /translation="MFPITRLRRLRYNATLRNMVQETQLHHSNFIMPVFIHYGKNVKQ
                     EIASMPGIYQFSIDLAIEECKRLYNIGVQSIIVFGIPEHKDETGECACTENGIVQQAI
                     YAIKKAVPQLYIIADVCNCEYTTHGHCGTIVNNDVQNDMTLVTLSKQSVSLVQAGADM
                     VAPSDMMDGRVLAIRSALDESGFEQIPILSYAVKYASAFYGPFRDAAQSSPQFGDRKT
                     YQMNPANSREAIREIETDIAEGADIVMIKPALSYLDIIHTVKQSCNIPVFAYNVSGEY
                     AMVKAAAKQGWIDEQRIVFEILTSIKRAGADCIISYHTQDIIELLP"
BASE COUNT         1408 a          664 c          603 g         1202 t
ORIGIN      
        1 attcgtgaaa aatttgcact ttcaaattcc gaaataccaa catttgccga ggaactccaa
       61 aaacaagcta atttttctga tattgttgta atttctactt gtaaccgaac agaaatatat
      121 ttttcacaac aaaaacacga tgggtttacc gcttcaatac ttctatttaa ttgtttgata
      181 gcttttaaaa aaattgattt tgctgcagaa cgctttttct atacttatat aaacgacgat
      241 gctgtatctc atttatttaa agtatgctca ggcatagatt caatgatatt aggcgaagac
      301 caaattattg gtcaagtaaa agaagcatat atttattgta cagaattagc attaacagat
      361 gccgttttaa tgcgtctttt tcaaaaatct tttgaagcag gtaaaaaggt acgtactcac
      421 actcaattaa atacaggtgt tacatcggtt agttttgcag ccattgaatt atgcaataag
      481 tatgtaactg attattctga taaaaccttg ctgcttatag gtacaggcga aaccggcaca
      541 ttagcattac aaggattcgt agaaaaaggc attcaaaata ttctaatatc taacagaacc
      601 cctgaacatg cagaaaaatt gattcataca tactctgcag aatttattcc tatccataat
      661 ttattagaat ctctacataa agctgatatt attataacag caaccaatgc aggacatact
      721 tttattacca aacaaagtat catagattct ttaaaaaaaa gacagaatat acctcaagta
      781 tatatagatt tatctgttcc tcgcaatata gaagaaagta ttagagatta tagtctaaca
      841 acacttatat gcgttgatga tattcaacat gtaattgata caaataaaga taagcgtgcc
      901 gaacaaattg aacatgcaca accaataata gacgaacttg ttcaagaatt tatggaatgg
      961 ctttcgtttc gtgctttaca acctgctata cgtgccataa attttcattt acaaaaaata
     1021 tatcgtgatg aatttcatgg gttcattgtt gataattcaa aagatgtaaa agaaaatata
     1081 aaggattttg caggatattt aacccaacaa tacagccgtg aattaataaa aaatctcaaa
     1141 gaaattaccg ataatggtcg taaagaattg tatttacaag ttattaatga tttatttaga
     1201 cttgaacgct aaaacaaaaa catagtgaaa attacaatag gaacacgagg aagtaaactt
     1261 gcactttggc aatcaaattc agtaaagcaa ttacttgaac aagcttttcc gcatattacc
     1321 attcaattac aaataattca aactaccggt gataaaatac ttgatgtagc gttatcaaag
     1381 attggagata aaaatctttt taccaaagaa attgaaactg ctatgctccg gcatgaagtt
     1441 gacattgcag ttcatagttt aaaagattta ccaacccaac tacccgaagg attatgtgta
     1501 gctgcagtat tacctcgagc tgaatgtaga gacgcattac tgagtaaaac aggtaaaact
     1561 attcatgaat taactccaca tgatattatt ggtacatcat cactccgtcg caaagctcaa
     1621 ttacttgcat ttaacccaca attaactatt catgatatac gaggtaatgt tgataccaga
     1681 atacataaaa tgcaaagtgg acaatacgac gctcttatta tggctgctgc aggccttcaa
     1741 cgattacatt attctcatga aattacccac attatagaac ctactataat gcttcctgca
     1801 gtaagtcaag gtataatagg tatagaatgc cgtagtaacg atactcatat tcgtgaaata
     1861 cttcaaacta ttcatgactc taaaacatgg tatatggctg cagctgaacg tacattttta
     1921 gaacaaatga atggcggttg ccaattacca ttgggttgtt ataccagtat ttctcaaaat
     1981 actattacat tacaagctgc tattttatca gttgacgggt ccaaaaaact tgcattttct
     2041 cattcgggag atttacagca agcccaaaca gttgcaaaac aattagccca aacattttat
     2101 aatgcgggag ctcatgaaat tttgaatacc atacaacatg actaattctg ttttacataa
     2161 tactcgaatt ttacttaccg gtgctgccga aacttcacaa gaattagcag aaatactttc
     2221 actatatgga gctactgtaa aggcatcacc aatgatagaa attaaaaaaa ttgaaattac
     2281 cgatactatt ttaaagctat tcgggtcttt atctcaattt caatgggttg tatttaccag
     2341 taaatatggc gtttctacat tttttgacag tattaccccg aaacacatcc cacaacacat
     2401 tcaatgggct tgtgtaggaa aaaaaactgc tcaaaaatta acagattatg gatatatacc
     2461 acattttata tgcagtaaaa ataacggaaa agactttgct caagaatttt ctaattatat
     2521 taatggacaa tttgtttctg tgttttttcc aacaggaaat cttacagaaa atatgattac
     2581 cttatcagtt tctccaaata tttctattac aaaatgtatt atttataata ctgttatgcc
     2641 ttctacaatt gatacaaata ttttcactca aatagaacat aaacaatttg attatattat
     2701 atttacaagt ccttcgggag ttacaaattg tatgaaagca agcaataatt tacctatctg
     2761 gaattattgc accattatta gtataggtcc atctacaagc aaacgattac aaaaatatgg
     2821 ttgtacctct tttatacaag cagaagaata taattctcaa ggaataattt cagttttaca
     2881 acaacttaat agtaactagt atgtttccta taactcgact tcgccgttta cgctacaatg
     2941 caactttacg caatatggta caagaaacac aattgcacca ttcaaatttt ataatgcctg
     3001 tatttattca ttatggtaag aatgttaaac aagaaatagc ttcaatgccg ggaatatatc
     3061 aattttcaat agatttagcc attgaagaat gtaaacgatt atacaacatt ggtgttcaat
     3121 ctataattgt atttggaata cccgaacaca aagatgaaac aggcgaatgt gcatgtacag
     3181 aaaacggtat agtacaacaa gcaatatatg ccataaaaaa agcagttcct caattataca
     3241 tcattgccga tgtatgtaat tgtgaatata ctacacatgg gcattgtggc actatagtaa
     3301 acaatgatgt gcaaaatgat atgacacttg ttacattatc aaaacaatcg gtatcgttag
     3361 ttcaagccgg agctgatatg gtagcaccaa gcgatatgat ggatggccgt gtattggcaa
     3421 ttcgttcggc tttggatgaa tctggatttg aacaaattcc tattctatca tatgccgtaa
     3481 aatatgcttc tgcattttat ggtccatttc gcgatgcagc tcaaagttca cctcaatttg
     3541 gcgatagaaa aacataccaa atgaatccgg caaacagtag agaagctatt cgcgaaatag
     3601 aaacagatat agcagaaggt gccgatattg taatgataaa accggctcta agctacctcg
     3661 atataattca cactgtaaaa caatcgtgca acattccggt atttgcttat aatgtaagcg
     3721 gtgaatatgc tatggtaaaa gctgctgcta aacaaggttg gatagatgaa caacgaatag
     3781 tatttgaaat acttacatct atcaaacgtg ccggagctga ttgcattatt tcatatcaca
     3841 cacaagatat tattgaattg ttaccgtaat tttacac
//
LOCUS       DAOTOW010000122         3845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19010_length_3845_cov_3.377836, whole genome shotgun sequence.
ACCESSION   DAOTOW010000122 DAOTOW010000000
VERSION     DAOTOW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19010_length_3845_cov_3.377836"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="P5243_03095"
     CDS             complement(<1..1372)
                     /locus_tag="P5243_03095"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HRS18467.1"
                     /translation="MKKIIVFVCIVVWWGTTFSKDYVVSLTIPGFSGQQAYVARIEGD
                     MSYFVDTVQADLSGTFRSVFTDKSPIGMYSYVLPQLRNAEVFFIFNKETVRLQTEITQ
                     LKHVKVLESKENALYYEFIKQKLLLEANADILEYTFDHYTGKSYLELTKKEYIQLLTS
                     FMSTVEQLKTESKGLFVYRIIQSSMPVLPPTLVSAQEKKAYQIAHFFDAVDFSDTLLC
                     NTNIFTSACISYLGLFSEQYQKSKSNTVFKQAADVILSKTKPYKTTYDFVVNYLLNGF
                     ETMGATDVLQYISAQYLAENQCMEGQSKSTLQRKALSNTKLSVGTAIPEFTTNTVQGK
                     PFSNQDMQKGIYVLVFWASWCGHCTQMVPTIVLDYAKKKEPGYTLVTVSLDTVLSDYK
                     KFTSAIPEWNKTIQVCDTKKWDGAMSQAFFTYATPTIFIIQDGVIVAKPIEYDAYIKE
                     MKKLHLL"
     gene            1633..2109
                     /gene="rimP"
                     /locus_tag="P5243_03100"
     CDS             1633..2109
                     /gene="rimP"
                     /locus_tag="P5243_03100"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRS18468.1"
                     /translation="MNSIITYENIQNIVSSFIEGTDMFIVEIALSKSNNITVSIDSPQ
                     GVDIDTCVELSRYIEQHLDREQEDFELTVASAGLSEPFKVHKQYLKNIGKLVQVLTTE
                     HKKYIGTLTSVTEQEITLTYTELVKMGPKGKKKEVESTITIPFTGIKKTTLEIIFK"
     gene            2149..3408
                     /gene="nusA"
                     /locus_tag="P5243_03105"
     CDS             2149..3408
                     /gene="nusA"
                     /locus_tag="P5243_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRS18469.1"
                     /translation="MKIEHQNLIETFSEFKELKNINRPTMMHILEDVFRSMLKRKFNI
                     EEDEDIQKSFDIIINIDKGDFEIWHNREVVADDELENPLHQISLSDAKKIDEDYEIGE
                     DVTNEVKLEDFGRREVLSIRQNLISRIMDLEKNNVYNKYKDQVGEIVTGEVYQVWKKE
                     ILILDDEGNELILPKTEQIPSDFFRKGDNVRGVVARVEMRNGNPLVIISRTSPFFLER
                     LFELEVPEIFDGLITIKKIVRIPGEKAKIAVESYDERIDPVGACVGMKGSRIHGIVRE
                     LRNENIDVINYTANPQLFIQRALNPAKISSIVIDEAHKKAEVFMKPDQVSLAIGKGGT
                     NIRLASQLTGYELDVYRDNIEEEEDVNIEEFADEIDEWIIQEFKKVGCDTAKSILKLS
                     KDELLKRTDLEEETINEVLEILRAEFE"
     gene            3459..>3845
                     /locus_tag="P5243_03110"
     CDS             3459..>3845
                     /locus_tag="P5243_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRS18470.1"
                     /translation="MAIRLIKASREFNVGISTIVEFLEKKGHTIDSDPNSKIPDELYQ
                     LIEKEFQTEGSVKKAAKQIDLSETRKKQEVITISDVVEHTKQPEEATIETVIPEFEHK
                     LNVVGKIDLHQLKKGKPVKKEEPSPEK"
BASE COUNT         1497 a          600 c          590 g         1158 t
ORIGIN      
        1 ataacaaatg taattttttc atttctttta tatatgcatc atattctata ggttttgcaa
       61 caatcacccc gtcttgtatt ataaatatag taggtgtagc ataggtaaaa aaagcctgcg
      121 acatagctcc atcccatttc ttagtatcac atacttgtat tgttttattc cactccggaa
      181 tagcacttgt aaacttttta taatcagaca aaacagtatc aagcgataca gtaactaatg
      241 tgtatcccgg ttcttttttc tttgcataat ccaacacaat agttggaacc atttgagtac
      301 aatgcccaca ccacgaagcc caaaaaacca atacatatat tcctttttgc atatcttgat
      361 ttgaaaatgg ttttccttga actgtattgg tagtaaattc aggtattgct gtgcccacac
      421 tcagctttgt attactcaaa gcttttcgtt gcaacgtaga tttcgactgc ccctccatac
      481 attgattttc tgctaaatat tgtgccgaaa tatattgcaa cacatcggtt gcccccattg
      541 tttcaaaacc gtttaaaaga taatttacaa caaaatcata ggtggttttg tatggttttg
      601 ttttagaaag aattacatca gcagcttgtt taaatacggt attactttta cttttttggt
      661 attgttccga aaataaccct aaatatgata tacaagccga agtaaaaata tttgtattac
      721 acaacaatgt atctgaaaaa tcaacagcat caaaaaaatg agcaatttga tatgcttttt
      781 tctcttgagc cgacactaat gtaggtggta atacaggcat agatgattga attatacgat
      841 acacaaacag cccttttgat tctgttttca attgttctac agtactcata aaacttgtaa
      901 gtaactgtat atattctttt ttggttaatt ctaaatatga tttgccggta taatgatcaa
      961 aagtatattc caaaatatca gcatttgctt cgagcaacag tttctgtttg ataaactcat
     1021 aatacaatgc attttctttt gattcaagta ccttaacatg tttcaattgc gtaatttctg
     1081 tttgcaaacg taccgtttct ttattaaaaa taaaaaacac ttcggcattt cgtaattgag
     1141 gcaacacata cgaatacata ccaatagggc ttttatcggt aaaaacagaa cgaaaagtac
     1201 cactcaaatc agcctgcact gtatctacaa aatacgacat atcaccctct atacgagcaa
     1261 catacgcctg ttgcccgcta aaccccggta ttgtaagact aacaacataa tccttagaaa
     1321 atgtagttcc ccaccataca actatacaca caaaaactat aatttttttc atacaacttg
     1381 tattacttat tttttacgaa aatacatttt tttataatgc ttacaacatt attcattaca
     1441 aacccatccc tattttttaa cctctacaaa ataaattata caatcatata tacaactcgt
     1501 aatcatttaa tatattttcc tcaatttttt gagctatttt ttttgattat tacaagaaat
     1561 aatatatttt tgccatgaat tttcgaagaa tatgaagggg acaattcgtc ccttttttat
     1621 taaatataat ttatgaatag tataataaca tacgaaaaca ttcaaaatat agtatcttct
     1681 tttatagaag gtaccgatat gtttattgta gaaattgctt tatcaaaatc aaataatatt
     1741 actgtgagta tagattcacc acaaggagta gatatagaca cttgtgtaga attgagtaga
     1801 tacatagaac aacatcttga cagagaacaa gaagattttg aattaacggt tgcatcggca
     1861 ggattgtcag aaccatttaa agtacacaaa caatacctta aaaacatagg caaattagta
     1921 caagttctta caaccgaaca taaaaaatat ataggaacat taacatcggt aacagaacaa
     1981 gaaataacac ttacctatac cgaattggta aaaatgggac caaaaggcaa aaaaaaagaa
     2041 gtagaatcta ccataacaat tccatttaca ggcataaaaa aaacaacgtt agaaataatt
     2101 tttaaataat aattaattat acagtcattt tcaaaaaaaa atagagagat gaaaatagaa
     2161 catcaaaatt taattgaaac attttctgag ttcaaagaac ttaaaaatat caatcgtcca
     2221 actatgatgc acatattaga agatgtgttc agaagtatgc ttaaacgaaa atttaatatt
     2281 gaagaagacg aagatattca aaaaagtttc gatataatta taaatattga caaaggtgat
     2341 tttgaaattt ggcataaccg cgaagttgta gccgacgatg aattagaaaa ccctttacat
     2401 caaatatctc taagcgatgc aaaaaaaatt gacgaagatt atgaaattgg tgaagatgta
     2461 accaacgaag taaaattaga agattttgga cgaagagaag tgttaagtat tcgtcaaaac
     2521 cttatttctc gaatcatgga tttagagaaa aacaacgttt acaacaaata taaagaccaa
     2581 gtaggtgaaa tagtaacagg cgaagtttac caagtatgga aaaaagaaat tttaatactt
     2641 gatgacgaag gcaacgagct tatattacct aaaacagaac aaataccatc ggactttttc
     2701 cgcaaaggag ataatgtgcg aggtgtagtt gccagagtag aaatgcgaaa tggaaacccg
     2761 ttagttataa tttcacgtac atcaccattt ttcctcgaaa gattatttga attagaagtg
     2821 cccgaaattt ttgatggctt aattacaatc aaaaaaatag ttcgcattcc gggagaaaaa
     2881 gcaaaaatag ctgtagaatc atatgacgaa cgaatagacc ctgtaggtgc atgtgttggt
     2941 atgaaaggtt cacgaattca cggtattgtt cgcgaattgc gaaatgaaaa tattgatgta
     3001 ataaattata ctgcgaatcc acaattattt attcaaagag cactaaatcc ggcaaaaatt
     3061 tcatctatag taatagacga agcgcataaa aaagccgaag tgtttatgaa accggaccaa
     3121 gtatcattag caattggaaa aggaggaaca aatattcgat tagcaagtca acttaccgga
     3181 tatgaattag atgtatatcg tgacaatata gaagaagaag aagatgtaaa cattgaagaa
     3241 tttgctgacg aaattgacga atggataatt caagaattta aaaaagtagg atgcgataca
     3301 gcgaaaagta tattaaaact cagcaaggac gaattgctta aacgtacaga tttagaagaa
     3361 gaaacaataa acgaagtgtt agaaatatta cgagcagaat ttgaataaaa tcaaatgata
     3421 ttagtaacta tttttagtat gaattcaaag aaggtattat ggccataaga ttaataaaag
     3481 catcaagaga atttaatgta ggtatttcaa ctattgttga atttcttgaa aaaaaaggac
     3541 atacaattga ttcagatcca aattcaaaga ttcctgatga attgtatcaa ttaattgaaa
     3601 aagagtttca aacagaaggc tcagttaaaa aagcggcaaa acaaattgat ttatcggaaa
     3661 cccgaaaaaa acaagaagtt attactattt ctgatgttgt agaacatact aaacaacctg
     3721 aagaagcaac tatagaaact gtaattcctg aatttgaaca taaactaaat gtagttggta
     3781 aaattgactt acaccaacta aaaaaaggga aaccggtaaa aaaagaagaa ccttcgcctg
     3841 aaaaa
//
LOCUS       DAOTOW010000123         3836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19058_length_3836_cov_3.615181, whole genome shotgun sequence.
ACCESSION   DAOTOW010000123 DAOTOW010000000
VERSION     DAOTOW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19058_length_3836_cov_3.615181"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..427)
                     /locus_tag="P5243_03115"
     CDS             complement(5..427)
                     /locus_tag="P5243_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18471.1"
                     /translation="MKCEYAIIPEYRVIIEKLEGDVSIEESIAMSTKLFADPMYDTSY
                     TILVDIRNYTSVIDKTVLISNGEVFAQFARNNCKHGNTNVGMLISEPLHSVIVELFRN
                     NVLDLPLQISFFTTVEGALKYLHLLKYSKQIIATLSEM"
     gene            complement(548..1816)
                     /locus_tag="P5243_03120"
     CDS             complement(548..1816)
                     /locus_tag="P5243_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS18472.1"
                     /translation="MKEITAAEFDQLVLQGNKVVVDFYSTECPPCEALAPKFENMAEL
                     YGEHITFVKVYRQGNRELAEQLGVKSSPTVLFFDKGKQVADMISGGIKKSELVERLDA
                     MLPQETVANIKSKITKHTTYCDALIIGGGPAGLAAGLYLCQAKVNTILVDIQLPGGQV
                     STTHQVSNYPGFVEPQPGYMLSHHMSEQTRLCGTQYKVAVDVAHVDLHNKTVEIDAFE
                     TIQAKKIIIATGASPRPLGIPGEKEFKGRGISYCATCDAKYFNDKDVIVIGGGNSAVE
                     EADFITKFAKKVTMIHQFAEFTANKQAVEKIMANPKVSVLFQSEPRAFTKQGDTMIVE
                     YENLQTKERHTISADGIFVFIGMQPNTSLFGNQLQTDNWNYIVTDEDMQTNIPGVYAI
                     GDVRSKKYRQITTAVADGTIAAIHISKELM"
     gene            complement(1819..2373)
                     /locus_tag="P5243_03125"
     CDS             complement(1819..2373)
                     /locus_tag="P5243_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HRS18473.1"
                     /translation="MDCTKCNTKQCRTGTACKATKSNAEEIRAMYHESRNQDIVQAAA
                     QLVDNGRAGTLSRMQEIVEFAKTMQYKTIGLAYCYGMELQAAEVVYYFRNAGLHMVPV
                     SCTVGAHTQAEVNQQSCIHKVSCNPLSQVAQMQAEGVDFAITMGLCLGHDILFNKYIG
                     VDVTNLLVKDRVYNHQPLQALKTK"
     gene            complement(2377..3678)
                     /locus_tag="P5243_03130"
     CDS             complement(2377..3678)
                     /locus_tag="P5243_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS18474.1"
                     /translation="MYFSKHNIVSRIKDSDSFFIVNPLLQQADIVSAEEAKKIMAHDY
                     SLPALAEKKYVLPIEEESRLFRQKYLEFIDTRETDEVQLFFVPWYTCNFACSYCFQDE
                     YTNPNNISTPELIDSFFSYIHTAFAGRRKYITIFGGEPLLPGAQYKEIISTLIHKATE
                     QHIDIAVVTNGYHIAEYIDILQTGHIREIQVTLDGMQHMHDSRRMLRGGKPSFNTIVA
                     GIDKLLEYKIPVNLRMVVDKENIQELPKLAQFAIDKGWTQSPLFKTQLGRNYELHHCQ
                     TNNQRLYTRIEMYEDIYELCKTNPEILEFHKPAFSISKFLWEQGEMPSPLFDSCTGTK
                     TEWAFDYTGSIYACTATVGKTEERLGTFYPEISLNTDDIEAWQERDVLSIDACKQCNV
                     QLACGGGCAAVAKNNHGSVSAHDCRPITELLELGIGLYFKE"
BASE COUNT         1077 a          745 c          659 g         1355 t
ORIGIN      
        1 aatactacat ttctgataat gttgcaataa tttgtttgga atattttaat aaatgcaaat
       61 attttaatgc cccttcaaca gtagtaaaaa aagatatttg caaaggtaaa tctaaaacat
      121 tattccgaaa taattcaact attaccgaat gcagaggttc cgatataagc ataccaacat
      181 tagtattgcc atgcttacag ttattgcgtg caaattgtgc aaatacttca ccattagaaa
      241 taagtacagt tttatctatt actgatgtat aatttcgaat atctactaaa atagtataag
      301 aagtatcata catagggtca gcaaacagtt ttgttgacat agctatagat tcttctatac
      361 ttacatctcc ctctaatttt tctataatta ctctatattc gggaattatt gcatattcac
      421 atttcatacc ttctatttat atttcaagac tacccctatt agaaatatac aaataattta
      481 aatgttgtat tattcagtat atcttgaatc acaaatatag atgaaatatg taaaccattt
      541 tacattatta catcaactct ttagaaatat gaatagctgc tattgttcca tcggcaacag
      601 cagttgtaat ttgtctgtat tttttactgc gcacatcgcc tatggcataa accccgggta
      661 tatttgtttg catatcttcg tcagtaacta tatagttcca attatcagtt tgtaattgat
      721 ttccaaacaa cgatgtatta ggttgcatac caataaatac aaatataccg tcggctgaaa
      781 ttgtatgtcg ttctttggtt tgtaagtttt cgtattctac tatcatagta tcgccttgtt
      841 ttgtaaatgc acgtggttcg ctttgaaaca agactgatac tttggggtta gccataattt
      901 tttctactgc ctgtttgttg gcggtaaatt ctgcaaattg atgaatcata gtaacttttt
      961 tggcaaattt tgtaataaaa tctgcttcct caactgctga atttccacca ccaattacta
     1021 ttacatcttt gtcgttaaag tacttagcat cgcaggttgc acagtatgag atacctcgtc
     1081 ctttaaattc tttttcgccg ggtatgccaa gtggtcgtgg actggctccg gttgctatga
     1141 ttattttttt tgcttgaata gtttcaaatg catctatttc aactgtttta ttgtgcaaat
     1201 ctacatgagc tacgtctacc gcaactttgt attgagtacc acacaatcgt gtttgctcac
     1261 tcatgtggtg cgacagcata taacccggct gtggttctac aaagccggga taatttgaca
     1321 cttggtgcgt agttgatact tgtccgcccg gtaattgaat gtctaccaat attgtattta
     1381 cttttgcttg acacaaatat aatcctgctg ccaatcctgc aggaccaccg ccaattatca
     1441 acgcatcgca ataggtagta tgtttggtaa ttttagattt tatgtttgca acggtttctt
     1501 ggggtaacat tgcatctaac cgttctacta attccgattt tttaatacca cccgaaatca
     1561 tatcggcaac ttgtttccct ttgtcaaaaa acaataccgt tggcgaactt tttactccca
     1621 attgttctgc aagttctcta ttaccttggc gatatacctt aacaaacgta atatgctcac
     1681 cgtacaattc tgccatattt tcaaattttg gggcaagagc ttcacaggga ggacattctg
     1741 ttgaataaaa atctactact actttgttgc cttgcaacac tagttggtca aattctgctg
     1801 ctgtaatttc tttcatgact attttgtttt taatgcttgt aatggttgat gattgtaaac
     1861 tcgatctttt accaaaagat tggtaacgtc tacacctata tatttattga ataaaatatc
     1921 gtgtcctaaa cataatccca tagttatggc aaaatcaact ccttctgctt gcatttgagc
     1981 tacctgcgat aatggattgc atgatacttt atgtatacaa ctttgttgat ttacttctgc
     2041 ttgcgtatgc gcacctacgg tacacgatac cggaaccatg tgcaaaccgg cattgcgaaa
     2101 ataataaaca acttctgcag cttgtaattc cataccgtag caataggcaa gtcctatggt
     2161 tttgtattgc atggttttgg caaattctac aatttcttgc atacgcgaca gggttcctgc
     2221 cctaccgttg tctaccaatt gtgcagcggc ttgcactata tcttgattgc gcgactcatg
     2281 atacatggca cgtatttctt ctgcattact tttggtagct ttgcatgcag taccggtgcg
     2341 acattgttta gtattacatt tagtgcaatc catatcttat tctttgaaat acaatccaat
     2401 acccaattcc aataattctg taataggtct acaatcgtgt gccgaaacac ttccgtgatt
     2461 gttttttgca accgcggcac aacctccgcc acatgcaagt tgtacgttgc attgtttaca
     2521 tgcatctata ctcaatacat ctcgttcttg ccatgcttct atatcatctg tattgagcga
     2581 aatttcgggg tagaatgtcc ccaatcgctc ttcggttttg cctactgttg cggtgcaagc
     2641 atatatactt ccggtgtaat caaacgccca ttcagtttta gtgccggtac acgaatcaaa
     2701 caatggtgaa ggcatttcgc cttgttccca caaaaattta ctgatagaaa acgcaggttt
     2761 gtgaaattct aaaatttcgg gattggtttt acacaattcg tatatgtctt cgtacatctc
     2821 tattcgagtg tagagtcgtt gattgttggt ttgacaatga tgcaactcat aatttcgtcc
     2881 caattgtgtt ttgaaaaggg gcgactgtgt ccagccttta tctatagcaa attgtgccaa
     2941 ttttggtaat tcttgtatgt tttctttatc aactaccatt cgtaaattta ctggaatttt
     3001 gtactccaaa agcttgtcta ttccagctac aattgtattg aaacttggtt tcccaccacg
     3061 gagcattcgt cggctgtcgt gcatatgttg catgccatcg agtgttactt gaatttcgcg
     3121 aatatgtccc gtttgtaata tgtcaatata ttcagcaata tggtagccat tggttaccac
     3181 agctatatct atatgttgct cggtagcctt atgaattagg gtagaaataa tttctttgta
     3241 ttgagctccc ggtaacaaag gttcgccgcc aaaaatggtt atatattttc gtctacccgc
     3301 aaatgccgtg tgtatgtacg aaaaaaacga atcaattaat tcaggagtgc ttatattatt
     3361 gggattagtg tattcatctt gaaaacaata gctgcatgca aaattacacg tgtaccaagg
     3421 aacaaaaaac agctgaacct catcggtttc gcgtgtatct ataaattcca aatatttttg
     3481 tcgaaacagt ctggattctt cctctatagg taacacatat tttttttcgg caagagcagg
     3541 caacgaatag tcgtgtgcca ttattttttt tgcttcctcg gcacttacaa tgtcagcttg
     3601 ttgcaacaaa ggattcacta taaaaaatga atcagagtct ttgatacgtg atactatgtt
     3661 gtgttttgaa aaatacatgg aatattaatt aaatgattga aaaatgcgga gtaccattgg
     3721 cactccgcat tgttgttagt cgtggcaacc tgctactatt tttgaatttt tagcacagca
     3781 ttgtgctact ttcgtttcgt gagcttgagc cttttttact aatgatttca tacctt
//
LOCUS       DAOTOW010000124         3832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19084_length_3832_cov_3.218957, whole genome shotgun sequence.
ACCESSION   DAOTOW010000124 DAOTOW010000000
VERSION     DAOTOW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19084_length_3832_cov_3.218957"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..956
                     /locus_tag="P5243_03135"
     CDS             156..956
                     /locus_tag="P5243_03135"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRS18475.1"
                     /translation="MIKQRIVFVLLLLVSIVPTYADTCIPSRPQSFVYDEIGILQVEQ
                     KQELDRILQAFSTKTSTQIVVVIVNDLCGKDKAMYATELGEAWGVGSKEHDNGIVLLI
                     KPTGGQGQRQTFIAVGYGLEGVIPDAIAKRIVEQELIPRFKQNDIYGGIVAGITVLMQ
                     LTEKEYTAEEYVKKTEGDYSIFFLLLFILFAFILFTKFSEARKYARSNNVSLWVALLL
                     LQSMSNKHSGTYSNFRSGGGSFGNFGGGSSFSGFGGGSFGGGGAGGSW"
     gene            complement(967..2163)
                     /locus_tag="P5243_03140"
     CDS             complement(967..2163)
                     /locus_tag="P5243_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18476.1"
                     /translation="MKTRILICIVLFCLGGIQYSFSQTHAFTVYDTIPIRAGTNKFRL
                     YKLIEKWFETERGTRLIAKNRKELHFKGKGYFIYYNRVKIPDIFLSPHANERCKGSIV
                     FSIDVKIQDSIIVSKFTQFIHEAVYSEYGSMSFGMLMIYEKVPPGFCMEVEEWCNAVW
                     NDMKVKSEAEVKDRCTRIIPPILIRRRTYKVKEEEVKVVAEEKVDPQEYLKLDKYYNP
                     EDKTVKQVSTKTDDIPGITDVVRPENNGPIQKISKSKTTANYTQRNYNEQNTANDKPA
                     KEETVAIKPEEENNQEKQAEEKTKNQENTAKEKPAKKEKTVSKPADDYDQEEETKPTP
                     QKQAEAPKEKPAKETPAPKEKSSPAPKPEPAAKPAPAPKPAKAKPEPKKSKPGAADEY
                     DDYDDE"
     gene            complement(2245..3327)
                     /locus_tag="P5243_03145"
     CDS             complement(2245..3327)
                     /locus_tag="P5243_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRS18477.1"
                     /translation="MNVFRNYIISLTLVCFPMIAAHAFTVLSPQSEYIYYMGRFNTEN
                     NNEYICSFPASSMYIQTNSTIVKAHIQDYPLEQGRPNYIAIIVDGNVYKEFALSPKTT
                     EYTIFEQKNSVYHVIQICKRTESLIGSIGFKGITINKNASTKPVELPQKKILFIGNSI
                     TCGYGNEAPNATDSFTAHTENAYMSYAAIAARELQSQYHLIAYSGKGIYRNWADTVFD
                     IETMPKVFLKTLAFDTSNTWNHSRFIPTLIVLNLGTNDFSPPLGANKDLYIDRFTSFL
                     RTLLQLYPQVPIVCVNSQMLQEPQRSDQIAWIQESISLLKNNPIYFCMLSMQGPLGYG
                     ADWHPNIAQNKVNGMELVSFCKEMGW"
     gene            complement(3311..>3832)
                     /locus_tag="P5243_03150"
     CDS             complement(3311..>3832)
                     /locus_tag="P5243_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18478.1"
                     /translation="DTQVWKVRDIVSNIIVDTLDYTLYRVERVRRKADTLAWQPFTVY
                     GIREYSKSIVRIDDNIPYVILQFPEKQGKTWNGNEFNVKPAEQYTYTKINTNDTIQGI
                     IYDSVLVITQQKFKSLYTYQFKEERYAYGFGLISKIKYDVESQPNHANINLNLPIEQR
                     ITKGTIEIYERIP"
BASE COUNT         1124 a          570 c          706 g         1432 t
ORIGIN      
        1 aattctaaaa aggtaactat gtagcaggtt tggaaaaagc tattgcagaa gcaggtgagc
       61 aacttgcaac acattttccg tatcaatcaa atgatactaa tgagttaagc aacgaaattt
      121 catttgatgt ttaaatgttt gatatcctaa aatatatgat taaacaaaga atagtttttg
      181 tattactact gttggtaagt atagttccaa cctatgccga tacttgtatt ccgtctcgtc
      241 cgcaatcatt tgtatatgat gaaataggaa tattacaggt agagcaaaaa caagaattag
      301 atagaatact acaagcattt agtacaaaaa catcaacaca aatagtagtt gtaatagtca
      361 atgatttatg tggtaaagac aaagcaatgt atgctactga attgggcgaa gcgtggggtg
      421 ttggtagtaa agaacacgac aatggtatag tattattgat aaaaccaacc ggaggtcaag
      481 ggcaacgaca aacatttata gctgtaggtt acggactcga aggtgtaata cccgatgcta
      541 ttgcaaaacg tattgtagag caagaactga ttccccgatt taaacaaaat gatatatatg
      601 ggggtatagt agcaggtatt acggtattga tgcagcttac agaaaaagaa tatacggccg
      661 aagagtatgt aaaaaaaacg gaaggtgatt attcaatatt tttcttgctt ttatttatac
      721 tgtttgcatt tatattgttt acaaaatttt ctgaagcacg taagtatgca cgtagcaata
      781 atgtgagttt atgggtagca ttattgcttt tgcaatctat gtcaaacaaa cattcgggta
      841 catattctaa cttcagaagt ggtggaggga gttttggaaa ctttggagga ggaagtagtt
      901 ttagcggatt tggcggtgga agttttggtg gaggtggagc aggtggtagt tggtaaaatt
      961 atttgattat tcatcatcat agtcgtcata ttcatcagca gcacccggtt ttgatttttt
     1021 aggttcgggt ttagcttttg caggttttgg tgcaggagca ggttttgcag ccggttccgg
     1081 tttgggtgca ggacttgatt tttcttttgg tgcaggagtt tcttttgcag gtttttcttt
     1141 tggagcttct gcttgttttt gaggtgttgg ttttgtctct tcttcttggt cataatcatc
     1201 agcaggtttt gaaacagttt tttctttttt cgcaggtttt tcttttgccg tattttcctg
     1261 atttttagtt ttttcttctg cttgtttttc ttgattattt tcttcttcag gttttattgc
     1321 aacagtttct tctttcgcag gtttatcatt agcagtattt tgctcattat aattacgctg
     1381 agtatagttt gcagttgttt ttgattttga aattttttgt atagggccat tgttttcagg
     1441 tcttactaca tcagtaatgc ctggaatatc atcagttttt gttgatactt gttttactgt
     1501 tttgtcttcg ggattataat atttatcaag ttttaaatat tcttgcgggt caactttttc
     1561 ttctgcaact acttttactt cttcttcttt tactttatat gttcttctac gaatcaaaat
     1621 tgggggaatt attcgtgtac agcgatcttt tacttctgct tcagatttaa ctttcatatc
     1681 attccatacg gcattgcacc attcttcaac ttccatacaa aaacccggtg gcactttttc
     1741 gtaaatcata agcataccaa aactcatact accgtattct gaatatacgg cttcatgtat
     1801 aaactgggta aatttagaaa caattataga gtcttgaatt ttgacatcta ttgaaaatac
     1861 aatacttcct ttacaacgct catttgcatg agggctcaga aatatatccg gaatttttac
     1921 tctattataa taaataaaat atcctttgcc tttaaaatga agttcttttc tgtttttcgc
     1981 aattaatcga gtacctcgtt ctgtttcaaa ccatttttca ataagtttgt ataaacgaaa
     2041 tttattggtt ccggcacgaa taggtatagt atcgtataca gtaaatgcat gagtttgtga
     2101 gaacgaatat tgtattcctc ctaaacaaaa caaaactatg catataagta ttcgtgtttt
     2161 catacactca ttgtgatgat agaaaacgta aagttcgtgt tttttgttat gaaaaaaaaa
     2221 taaaaaacta tttcaatttt tttattacca acccatttct ttacaaaaac taactaattc
     2281 cataccattc actttgtttt gtgctatgtt ggggtgccaa tcggcgccat atccaagggg
     2341 gccttgcata ctcagcatgc agaagtatat aggattattt tttaatagtg aaatactttc
     2401 ttgtatccat gctatttggt cgctgcgttg tggttcttgc agcatttggc tgtttacaca
     2461 tactatagga acttgaggat acaattgtaa aagggttcgt aaaaatgaag taaaacggtc
     2521 tatatataaa tctttgtttg ctcctaatgg tgggctaaag tcattagttc ctaaatttag
     2581 tacaattaaa gttggtataa agcgagaatg attccatgta ttgcttgtgt caaaagctaa
     2641 tgtttttaga aatactttgg gcatggtttc tatatcaaac acagtgtcgg cccagttacg
     2701 ataaattcct ttgcccgaat atgctattaa atgatattgt gattgcagtt cgcgagcagc
     2761 aatagctgca tatgacatat aagcattttc tgtgtgagcg gtaaatgagt cagtagcatt
     2821 aggtgcttcg ttgccatagc cacaggtaat agaattgcct ataaatagta tttttttttg
     2881 tggtaattcc acaggtttgg ttgatgcgtt tttattaatt gtaattcctt taaatccaat
     2941 tgaaccaatt aaactttcag ttcttttgca aatttgaatt acatggtata cactgttttt
     3001 ttgttcaaat attgtatatt cagtagtttt tggagataaa gcaaattctt tatatacatt
     3061 accgtcaaca ataattgcta tatagttagg acgaccttgt tcaagcggat aatcttgtat
     3121 gtgtgctttt actatagttg aattagtttg tatatacata gaacttgcag gaaatgaaca
     3181 gatatattcg ttattatttt cggtattgaa tctacccata taataaatat attctgattg
     3241 tggtgaaagt accgtaaagg catgtgctgc aatcatagga aaacaaacta atgttaatga
     3301 aattatataa ttacggaata cgttcatata tttcaattgt accttttgta atacgttgtt
     3361 ctattgggag gtttaaattg atatttgcat gatttggttg cgattctaca tcatatttga
     3421 tttttgagat aagtccaaaa ccatacgcat atcgttcttc tttaaattga taggtatata
     3481 aagatttaaa tttttgttgt gtgataacca aaaccgaatc gtatattatt ccttgtatag
     3541 tatcatttgt gtttattttt gtatatgtat attgttcggc aggttttaca ttaaattcat
     3601 tgccattcca tgttttacct tgtttttctg ggaattgtaa aattacatac ggtatattgt
     3661 catctatacg aactattgac tttgaatatt cacgaatacc gtatacggta aatggttgcc
     3721 atgcaagagt atcggctttg cgacgaactc gttctacacg gtacaatgtg taatctaatg
     3781 tgtctactat tatattactt acaatatcac gaactttcca tacttgcgta tc
//
LOCUS       DAOTOW010000125         3829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19102_length_3829_cov_3.113937, whole genome shotgun sequence.
ACCESSION   DAOTOW010000125 DAOTOW010000000
VERSION     DAOTOW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19102_length_3829_cov_3.113937"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="P5243_03155"
     CDS             complement(<1..289)
                     /locus_tag="P5243_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18479.1"
                     /translation="MKKFLLLLLFVCFGFSTLYSQIFEGRIDVVKRSLSDTLYYTYSI
                     KGNFIRIDEYDKYKRLQEYYIINVKDSTITALNPNQKLYSKIDVQRLHASKQ"
     gene            340..717
                     /locus_tag="P5243_03160"
     CDS             340..717
                     /locus_tag="P5243_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRS18480.1"
                     /translation="MQYKNTQKTELGAKGEQIACKYLTSHGYKILERNWRFSHKEIDI
                     IAKKENTIHVVEVKTRATTFFERPQDAVTKNKQRFLIAAINNYIQNQPFNGDIQFDII
                     TVLVQPNSETIEYIPNAFYPLCR"
     gene            complement(718..1455)
                     /gene="pgeF"
                     /locus_tag="P5243_03165"
     CDS             complement(718..1455)
                     /gene="pgeF"
                     /locus_tag="P5243_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRS18481.1"
                     /translation="MMKFQTIQSIPYFQYIDSPLCIAGVSTRNGGISKGVYSSMNLGT
                     HTADNPTHVQHNRQLFFGTIAPGFTVAYMRQTHSNIVLNVDSDFVNDTEGDAFYTTKK
                     GVLLTVSLADCGSVIIHDDSYSLIAAIHCGWRGVHSQIIEKTISELSAYVKPEKLTAY
                     IGPTIQQQYYEVGKEFLDLFPHSYFKENGEKLYFNLNGRIEDVLLESGVGMIMNSRMG
                     TFAVPEHFYSYRRDGATGRFCAFIGIV"
     gene            1613..3214
                     /locus_tag="P5243_03170"
     CDS             1613..3214
                     /locus_tag="P5243_03170"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRS18482.1"
                     /translation="MAEFTYHKMFQTGEDKTKYRLLTKNYVSTIQVEGRTIVKVDPKG
                     LELLAKEAIADVSFYLRSEHLQSLAGIIKDPETSDNDKFVADTMLRNQVVAAEGELPT
                     CQDTGTAIVIAKKGEGVWTGVNDEEYLSKGIFETYQEKNLRYSQVVATTLFDEKNSGN
                     NLPAQIDIMATYGNEYEFLFITKGGGSANKTYLYQETKSVLNEESFTKFIRTKIKDLG
                     TSACPPYHIAFVIGGQVDTNLKTVKLASAKYYDNLPTKGNEGGIAFRDLEWEAKIQKI
                     AEELGMGAQFGGKAFAHDIRVIRLPRHAASCPIGMGVSCSADRNIKAKITADGIFLEE
                     LEKNPAKYLPEAELMLTPAVEINIDQPMDEVRKILTKYPIKTRLSLTGTLVVARDIAH
                     AQFKKMLDEGKPLPEYLKKHPVYYAGPAKTPKGMASGSFGPTTAGRMDSYVDLLQSLG
                     GSLIMVAKGNRSPQVREACKKYGGFYLGSIGGPAAILAKESIKSSEVIDFEELGMEAV
                     RKIYVENFPAFIICDDKGNDFFLQF"
     gene            complement(3292..>3829)
                     /locus_tag="P5243_03175"
     CDS             complement(3292..>3829)
                     /locus_tag="P5243_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18483.1"
                     /translation="ENSNGYQVGYLRLLSKYVTSTAPQTQTIQLSQGWNIISLYIDPD
                     NKDVSTMFPVTYTIKTQDGFYSTKNTKALSSIDTIVGGKGYVVYVASAQTFTRVGKPT
                     SKQSIQLKKGWNLIGFPYSESKNIATVIAPIASKVEYVKNFEGFMQPGGSTNSITNFQ
                     PGVGYFIKVNADCTLVWE"
BASE COUNT         1371 a          648 c          629 g         1181 t
ORIGIN      
        1 attgtttact tgcatgcaaa cgttgtacat caattttaga atacaatttt tgatttggat
       61 tgagtgcggt aattgtgctg tcttttacat tgataatata atactcttgt aaccgtttat
      121 atttatcata ttcatctatt cgtataaaat tacctttaat actatatgta taatataacg
      181 tatcggataa tgatcgttta accacatcta tacgtccctc aaaaatttga gaatacaatg
      241 tagaaaaacc aaaacataca aatagtaata aaagtaaaaa tttcttcata tttttacaaa
      301 aataacccaa ctatcaaaaa taagaaataa gtcccaacaa tgcaatacaa aaatacacaa
      361 aaaactgaat taggtgctaa aggtgagcaa attgcgtgta aatacctaac ctctcacggg
      421 tacaaaatac tcgaacgaaa ctggcgattt tcacataaag aaattgatat aattgccaaa
      481 aaagagaata ccatacacgt ggtagaagta aaaactcgag caactacatt ttttgaacga
      541 ccacaagatg ctgtaacaaa aaacaaacag cgttttttaa ttgctgcaat aaacaattac
      601 attcaaaatc agccttttaa tggtgatata caatttgata ttataaccgt attagtacaa
      661 cccaattcag aaactataga atatattcca aatgcattct atccactctg ccggtaatta
      721 tactatacct ataaatgcac aaaatctacc ggttgcacca tcgcgtcggt acgaatagaa
      781 atgttcgggt acggcaaatg ttcccatacg tgaattcatt atcattccta caccactttc
      841 aagcaacaca tcttctattc ttccgtttaa attaaaatat aatttttcac cattttcttt
      901 gaagtatgaa tgaggaaata aatcaagaaa ttcttttcct acttcataat attgctgttg
      961 tatagtagga cctatatatg ctgtaagttt ttcaggttta acatatgcac tcaattccga
     1021 aatggttttt tctattattt gcgaatgtac accacgccag ccacaatgaa tagcggctat
     1081 taaactatat gagtcatcat gtataatcac cgaaccgcaa tctgccaagc ttaccgtaag
     1141 taaaacacct tttttggtag tataaaatgc atctccttcg gtatcattta caaaatcgga
     1201 atctacattc agcactatat ttgaatgagt ttgacgcata tatgcaaccg taaatccggg
     1261 tgctattgta ccaaaaaaca actgtctgtt gtgttgtaca tgtgtaggat tatctgcagt
     1321 atgtgtaccc aaattcatag aactatatac tcccttgctt ataccaccgt tgcgagtact
     1381 aacacctgct atacaaagag gactgtcaat atactgaaaa tacggtatag attgaatggt
     1441 ttgaaatttc atcatagggt ttatatgaat ttaatagggt tgtaaaacat aggttttatt
     1501 ctattactaa gtgcttaaaa atacaaaaaa tattgaccta aaactacaaa aaaatttatt
     1561 tttttatctt acgtttgtag aaactattaa caaaacaaaa aattatataa ttatggcaga
     1621 atttacatat cacaaaatgt ttcaaacagg cgaagacaaa acaaaatatc gcttacttac
     1681 caaaaactat gtttctacca ttcaagtaga aggcagaact atagtaaagg ttgaccccaa
     1741 aggattggaa ttacttgcta aagaggctat agccgatgtg tcgttttact tacgaagcga
     1801 acatttacaa agtttggctg gcataataaa agaccctgaa acatctgata atgacaaatt
     1861 tgtggcagac actatgcttc gtaaccaagt agtggcagcc gaaggcgaat tacctacttg
     1921 ccaagataca ggaacagcca tagtaatagc aaaaaaaggc gaaggggtat ggaccggagt
     1981 aaatgacgaa gaatatctat caaaaggaat ttttgaaaca tatcaagaaa aaaacttacg
     2041 atattcacaa gtagttgcta caacattgtt tgacgaaaaa aattcaggca ataatctacc
     2101 tgctcaaata gatataatgg caacttacgg taatgaatat gaatttttat ttattaccaa
     2161 aggtggtggt tctgcaaaca aaacatattt atatcaagaa acaaaatcgg tgctcaatga
     2221 agaatcgttc acaaaattta tacgtactaa aattaaagat ttaggcacat cggcttgtcc
     2281 tccttatcat atagcgtttg taataggcgg acaggttgat accaatttaa aaacagtaaa
     2341 attagcttcg gctaaatatt atgacaattt accaaccaaa ggtaatgaag gtggaattgc
     2401 attcagagat ttggaatggg aagcaaaaat tcagaaaatt gctgaagaac ttggtatggg
     2461 agcacaattt ggcggtaaag cttttgctca tgatattcgt gtaattcgat tgcctcgtca
     2521 tgctgcatct tgtccaatag gaatgggtgt aagttgtagt gccgaccgca acataaaagc
     2581 aaaaattaca gccgatggaa ttttcttgga agagttagag aaaaatcctg caaaatattt
     2641 gcccgaagct gaattaatgc ttactcctgc agttgaaata aacatagacc aacctatgga
     2701 tgaagtaaga aaaatattaa caaaataccc tattaaaacc cgattaagtt taacaggaac
     2761 attggtggtt gctcgtgata tagctcatgc tcaattcaaa aaaatgcttg atgaaggtaa
     2821 accgttacct gaatatttaa aaaaacaccc cgtgtactat gccggtccgg ctaaaacacc
     2881 taaaggtatg gcttcgggta gttttggtcc aactacggcc ggtcgtatgg attcatatgt
     2941 agatttatta caaagcttag gtggttcact tataatggtt gctaaaggaa accgttcacc
     3001 acaagtacgc gaagcttgta aaaaatacgg aggtttttat ttaggttcta ttggtggccc
     3061 tgcggcaatt ttggcaaaag aaagtataaa atcatcggaa gttatagatt ttgaagaatt
     3121 gggaatggaa gctgtgcgca aaatttatgt agaaaacttc ccggcattta ttatatgtga
     3181 cgataaaggt aatgacttct ttttgcaatt ttaatcactg ctaaattata tctaaaactg
     3241 tattttggaa caataaatat gttccaaaat acagtttttt tatatctact attattccca
     3301 caccaaagta caatcggcat ttactttaat aaaataacca actcccggtt gaaagtttgt
     3361 tatactattg gtagaaccac cgggttgcat aaatccttca aagtttttca catactctac
     3421 ttttgaagca ataggagcta taactgtagc aatattttta gattcactat agggaaatcc
     3481 tattaaattc catccttttt taagttgaat gctttgtttg gaagtaggtt tgcctactcg
     3541 tgtaaatgtt tgagcagatg ctacataaac aacataacct ttacccccca caatagtatc
     3601 aatactacta agagcttttg tatttttagt agaataaaat ccatcttgag tttttatggt
     3661 atatgtaacc ggaaacattg tagaaacatc tttattgtca ggatcaatat acaatgatat
     3721 aatgttccac ccttgcgata attgaattgt ttgagtttga ggagctgtag atgttacata
     3781 ttttgataat aatcgtaaat acccaacttg atatccatta ctattttcg
//
LOCUS       DAOTOW010000126         3813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19201_length_3813_cov_3.441990, whole genome shotgun sequence.
ACCESSION   DAOTOW010000126 DAOTOW010000000
VERSION     DAOTOW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19201_length_3813_cov_3.441990"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /locus_tag="P5243_03180"
     CDS             complement(<1..2058)
                     /locus_tag="P5243_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18484.1"
                     /translation="MNNNKLKAIFTSIFLIVCAIGFAQTYYSKSTGDLHNVSTWGQNS
                     DGTGTAPANFTSNNRTYIIANRTSATLTANWTVSGTSSKVIVGNSNDSIIFSIPSNYT
                     YTGTIDVSNKAKLEIANTTNPTLGTMNDGSTVVFCSKENQNIPAVSYYNLHTEIGRTK
                     SLTNHTTVRNKLRIGAITTFNAASYNLTLSGNETIVTLIGIFDPGTSTVLYTNTSSTT
                     VCAMDYHNLNISGGPRILQNKNIIGIKGTFTTGSGPFTISNSTIEYKGSSAQNITNFT
                     YYNLRINNSAGLTMAGGASTITNNITFINGKVTTSPTNTLTVSNPSQDAVSYGNSSSY
                     IIGTFTRSIEANLSNSMNRYIFPVGNSSNYYEFILSKITTGVVVPIISVTYNASNEWA
                     TSISTGSVSSTSISVTLPSTPASNACILYSSTSGGSGTNIFGTVSGNSIVYTTNAALQ
                     NFYRTSTRPVSTQTYVYCSGDVHNANNWKLTNCSGTALPNFTGDDRVYVINNTSATLT
                     SNLAITGHNVTFQIGSGSATSLTVNSGVTLSLPGNILMQQGSTLTNNGTITAAGNISY
                     SNFGSLNYIDIQNNGLITVYGNITQNGGSYSRIYNNATGIINLYGNYTKDQNTWFSNS
                     GLWNINSGSFSSTNNNQNYFTNEIGGIVNVYNKLAQTKTVTFNNIFTSDGFYNKTNSI
                     FNVE"
     gene            complement(2264..3100)
                     /locus_tag="P5243_03185"
     CDS             complement(2264..3100)
                     /locus_tag="P5243_03185"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HRS18485.1"
                     /translation="MNKLQKQSQHTQYTQYMSKGILHKMHTQLGDTCVQYSLDLGEKN
                     ISLNSLVQKHITITHTGNIYCIECGCKTNKSFAQGYCYRCFTTSPHTEECVLHPELCQ
                     AHIGIARDMEYAQNHCLIPHYVYVAETSDIKIGITRNTQIPTRWIDQGALQACIIAQT
                     PNRYTAGVLEVALKQKVADKTNWRKMLVKNTPEYDLRHEISVIKKYVPEYSKFLIHSE
                     IQTLVYPGSIAFAKIQSVKLDTKPSVSGILRAIKGQYLIFESGEVLNIRSHGGYEVTI
                     TE"
     gene            complement(3045..>3813)
                     /locus_tag="P5243_03190"
     CDS             complement(3045..>3813)
                     /locus_tag="P5243_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HRS18486.1"
                     /translation="TYLANAVELARKNDSKGILELLKKAVDDYNELVIPQCPRPQIGI
                     VGEIYLKYNPYANMFVTDWLIQQGIEVRFPDLLDFFIQEFVNMKINHEKNLSKTTWGT
                     KLYVGFLENKFEKYCKKVEKIASNFNYYRPKHSIYQESKNASEILDLANQFGEGWLIP
                     AEIISFAQEGVNNVVSLQPFGCIANHIVAKGVEKRIKTLYPQTNLLFLDFDGGTSEVN
                     VFNRLHFLAQHAKTEFMQNHEQTSEAESTHTIHTIHE"
BASE COUNT         1060 a          595 c          718 g         1440 t
ORIGIN      
        1 ttctacatta aaaatactat tagttttatt ataaaatcca tcgcttgtaa aaatattgtt
       61 aaatgtaact gtttttgttt gagctaattt attataaaca tttacgattc ctccaatttc
      121 attagtaaag tagttttgat tattgtttgt tgatgaaaaa ctgccactat ttatattcca
      181 taatcccgaa ttactaaacc atgtgttttg atctttggta taattaccgt ataaatttat
      241 tattcctgta gcattattgt aaattcttga atatgatcct ccattttgtg taatgttacc
      301 atatacagta attaatccat tgttttgaat atcaatatag tttaaagatc cgaaattgga
      361 atacgaaata ttacctgccg ctgtaatagt tccgttgttt gtgagtgtag agccttgctg
      421 cattagaatg tttcccggta atgatagtgt tactcccgaa tttacagtaa gacttgttgc
      481 agaaccgctc ccaatttgaa acgtaacgtt gtgaccggta attgcaagat ttgatgtaag
      541 agtagcgctt gtattattta ttacatacac tctatcgtcg cctgtaaaat taggtaaggc
      601 tgtgccggaa caattggtta gtttccagtt gtttgcattg tgtacatctc cactgcaata
      661 aacatatgtt tgtgttgaaa ctgggcgggt agaggttcta taaaaatttt gcaatgcggc
      721 attggtcgta tatactattg aattacctga aactgtcccg aaaatatttg taccggaacc
      781 tccagatgtg ctcgaatata atatacaagc atttgatgcg ggtgttgacg gtaatgttac
      841 ggaaatagat gtagaactta cagagcctgt tgaaatgctt gttgcccatt cattagaagc
      901 attatatgtt acacttatta tgggaactac tactcctgtt gtaatttttg aaagaataaa
      961 ttcgtaatag tttgatgaat taccaacggg aaatatatat cgattcattg aattacttaa
     1021 gtttgcttca atcgagcgag taaatgtgcc aatgatatac gatgaagaat ttccataact
     1081 aacagcgtct tgacttggat tactaaccgt tagtgtattt gtaggagatg ttgtaacttt
     1141 accgttaata aatgttatat tatttgttat cgttgacgct cctcccgcca tagtaagtcc
     1201 cgcgctattg ttgatgcgta aattgtaata ggtaaaattt gtaatgtttt gagctgaact
     1261 tcctttgtat tctatggtac tgtttgaaat agtaaaggga ccactcccgg tggtaaatgt
     1321 tccttttatg cctataatat ttttgttttg aagaatgcga ggccctcccg agatatttag
     1381 attgtgataa tccatagcac aaactgttgt acttgaagta ttggtgtata gaactgtaga
     1441 tgtgccaggg tcaaagattc cgataagtgt tactatggtt tcattacccg aaagtgtgag
     1501 gttgtagctt gcagcattga atgttgtaat tgcacctatt cgaagtttgt tgcgaacagt
     1561 agtatggttt gttaaacttt ttgttcgtcc aatttctgta tgcaaattat aataagaaac
     1621 agccggaata ttttgatttt ctttgctgca aaaaactaca gtacttccgt cattcattgt
     1681 gcctagggta gggttggttg tatttgcaat ttctaatttc gctttgttag atacatctat
     1741 tgttcctgta tatgtgtaat tcgatggtat agaaaaaata attgaatcat tagagttgcc
     1801 aactattact ttgctactag taccacttac tgtccaatta gctgtaagtg ttgcacttgt
     1861 tctatttgct attatgtatg ttcggttgtt tgaagtaaaa ttagccggag ctgttcctgt
     1921 gccatcagaa ttttgccccc aggtgcttac attatgtaaa tcaccagtag attttgaata
     1981 atatgtttgt gcaaaaccta tagcacatac tatcaaaaat atggacgtaa atattgcttt
     2041 taatttgttg ttattcattt tggtaggtat aatgttttat gcaaaaataa caaaattcat
     2101 acatataaat acacaatttt ataaaagtta ttaacttttt gtattttttt tgtaacttat
     2161 atgttttctg tacgtaagtc gttcgttttt gacggtgaaa tcaaatgttt cttacaagaa
     2221 aaatgagggg aaatttagat aaaattgctt gatttgtaga aggttattcg gtaatcgtaa
     2281 cctcgtagcc accgtggctg cggatattga gaacttctcc gctttcgaat ataagatatt
     2341 gtccttttat tgctctgagt attcccgaca ctgatggttt tgtgtcgagt tttactgatt
     2401 gaatttttgc gaaagctatg ctgcctgggt atactaaggt ttgaatttct gagtgtatga
     2461 gaaattttga atattcggga acatattttt ttattacaga tatttcatgg cgtaaatcgt
     2521 attcgggtgt gttttttacg agcatttttc gccaattggt tttgtctgca accttttgtt
     2581 ttaatgctac ttctagaact cctgcggtat agcggttggg tgtttgtgct attatacatg
     2641 cttgtagagc tccttgatca atccagcggg ttggtatttg tgtgttgcgt gttataccaa
     2701 tttttatgtc gctggtttct gctacataca cataatgggg gataaggcaa tggttttgtg
     2761 catattccat atctcgagct atcccaatgt gtgcttggca taattcgggg tgtaatacac
     2821 attcttcggt atgtggcgat gtggtaaagc aacgataaca atagccttgt gcaaacgatt
     2881 tgtttgtttt acatccacat tctatacagt atatattacc tgtatgagtt attgttatat
     2941 gtttctgtac aagggagttt aaactaatat ttttttctcc aaggtctaat gagtattgta
     3001 cacatgtatc gccaagttgt gtatgcattt tatgtaaaat acctttactc atgtattgtg
     3061 tgtattgtgt gtgttgactc tgcttctgaa gtttgttcat gattttgcat gaattctgtt
     3121 ttggcatgtt gtgccaaaaa gtgtaatcgg ttgaatacgt ttacttcgct tgtacctccg
     3181 tcgaagtcaa gaaacaataa attggtttgc ggatacaatg tttttattcg tttttctact
     3241 cctttggcaa cgatatgatt tgcaatacat ccaaatggct gtaagcttac cacgttgttt
     3301 actccttctt gtgcaaacga aataatttcg gcaggaatca accagccttc accaaattga
     3361 ttggctaaat caagaatttc cgatgcgttt ttagattctt gatatataga atgtttgggc
     3421 cgataatagt tgaaatttga tgctattttc tctacttttt tgcaatattt ttcaaatttg
     3481 ttttccaaaa atccaacata taattttgtt ccccacgtgg ttttcgaaag atttttctca
     3541 tgatttattt tcatgtttac aaattcttgt ataaagaaat ctaacaaatc ggggaatcgt
     3601 acttctatgc cttgttgaat caaccaatcg gttacaaaca tgttggcata cggattatat
     3661 tttaggtaaa tttcgcctac gatacctatt tgaggtcgag gacattgggg tataactaat
     3721 tcgttgtaat catcaaccgc ttttttcaaa agctctaata ttcctttact gtcgtttttg
     3781 cgagccaatt ctactgcatt tgccaaatag gta
//
LOCUS       DAOTOW010000127         3810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19221_length_3810_cov_2.880426, whole genome shotgun sequence.
ACCESSION   DAOTOW010000127 DAOTOW010000000
VERSION     DAOTOW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19221_length_3810_cov_2.880426"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1655
                     /locus_tag="P5243_03195"
     CDS             <1..1655
                     /locus_tag="P5243_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRS18487.1"
                     /translation="LGYVEVLPEDKWESKLVINKKPAKAMIAKYDTTEKVMAAYHELR
                     SYWDNLLGTITIKSHDERLDRMVNIWNQYQCMVTFNMSRSASFFESGIGRGMGFRDSN
                     QDLIGFVHQIPSRARERIIDIASTQFEDGSCYHQYQPLTKKGNAAIGGDFNDDPLWLI
                     LSTTEYIKETGDYSILDEMVPFDNDESKAKTHFEHLLISFNFTVNNLGPHGLPLIGRA
                     DWNDCLNLNCFSSEPNESFQTTGNKKGRTAESLMIAGLFVVYGREFIKLCKQIGKHAE
                     AAEAQKHVDAMVEAVKQHGWDGEWYLRAYDYFGRKIGSNENEEGKIFIESQGWCSMAE
                     IGIEDGLVEKSLNAVKERLDCEYGIVLNNPAFTKYYIEYGEISTYPAGYKENAGIFCH
                     NNPWIMIGETLIGRGDRAYEYYTKIAPSFLEDISDLHKVEPYVYCQMIAGKDAFKPGE
                     GKNSWLSGTAAWNFYTIVQYILGVKPDYNGISINPCIPKTWDGFSMKRVFRGATYVIE
                     VSNPNHVSKGVKQIIVNGNTIEGTIVPILEAGKTHTVQVIMG"
     regulatory      1873..2077
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2189..>3810
                     /locus_tag="P5243_03200"
     CDS             2189..>3810
                     /locus_tag="P5243_03200"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRS18488.1"
                     /translation="MVTKDVLKNRQISKRDGRIVPFEAEKITFAIFKALRAVGRPNRE
                     LAEDLMLDVLKQLCMFDKPNYTPSVEEVQDTVEKALFENKLFDVAKAYILYRKQHEHI
                     RNAKELFSNLDIVEKYLNLDDWRIKESANSSYSLQGLNQHIATIISSQYWLERIYSDE
                     VKQAYQTGRFHIHDLGFLSVYCVGWDLEDLLITGFKGVSGKIESRPAKHLRTILGQIV
                     NFFYTMQGEAAGAQAFSNFDTYLAPFIRYDKLNYAQVKQALQEFMFNINVPTRVGFQT
                     PFTNITLDLKIPEFLKNQPVIIGGKPQETVYGDFRAEMDIFNAAFAEVMAEGDANGSV
                     FSFPIPTYNITKDFEWDNPAYTKIWEIAAKYGIPYFSNFVNSDMNPDDVRSMCCRLRL
                     DKRELKKRGGGLFGANPLTGSVGVVTINLPRLGYESKSETEFFQRLDKLMELAKESLE
                     VKRKVIENLTERGLYPYSKFYLRHVKQRTGKFWKNHFSTIGIVGMNECCLNFLGCNLV
                     SEKGYAFGLKIMDTMRLKLEMFQDETGNIYNL"
BASE COUNT         1310 a          635 c          713 g         1152 t
ORIGIN      
        1 tgcttggcta tgttgaagta ttacccgaag acaaatggga aagcaaactg gtaatcaaca
       61 aaaaaccggc aaaagcaatg attgctaagt atgacactac tgaaaaagta atggctgctt
      121 accatgaatt acgttcatat tgggataatt tattgggcac aattacaatt aaatctcatg
      181 atgaacgatt agatagaatg gtaaatattt ggaaccaata tcaatgtatg gttacattta
      241 acatgagccg ttcggcttca ttctttgaat caggaatagg acgaggtatg ggattccgtg
      301 acagtaatca agatttaatt ggctttgttc accaaattcc aagtcgtgcc cgcgaacgta
      361 taattgatat agcatcaaca caatttgaag atggttcatg ttatcaccaa tatcaaccat
      421 taactaaaaa aggtaatgct gccataggtg gtgattttaa cgatgatccg ctatggttaa
      481 ttctttcgac tactgaatat atcaaagaaa ccggcgatta cagtatttta gacgaaatgg
      541 ttccttttga caatgatgaa tcaaaagcaa aaacacattt tgaacattta cttatttcat
      601 ttaattttac tgtaaacaat ttaggaccac acggattacc tcttattggt cgtgccgatt
      661 ggaacgattg tttaaatctc aattgtttct caagcgaacc aaatgagtcg ttccaaacaa
      721 caggtaataa aaaaggtcgt acagcagaat cacttatgat tgccggacta tttgtggttt
      781 acggtcgtga attcattaaa ttatgcaaac aaatagggaa acatgccgaa gctgccgaag
      841 ctcaaaaaca tgtagatgca atggtagaag ctgttaaaca acatggttgg gacggagaat
      901 ggtatttgcg tgcatacgat tattttggtc gtaaaatagg aagcaacgaa aacgaagaag
      961 gtaaaatatt tattgaatcg caaggttggt gtagtatggc cgaaattggt attgaagatg
     1021 gtttggttga aaaatcactc aatgccgtaa aagaacgcct tgattgtgag tatgggatag
     1081 tattgaacaa tcctgcattt actaaatatt atattgaata cggcgaaatt tctacttatc
     1141 ctgccggtta caaagaaaac gctggtatat tctgccacaa taacccatgg ataatgattg
     1201 gcgaaactct tataggtcgt ggagatagag catacgaata ctacactaaa attgcaccta
     1261 gtttcttaga agatatttct gacctacaca aagtagaacc atacgtatat tgccaaatga
     1321 ttgcaggtaa agatgctttc aaaccgggag aaggtaaaaa ctcatggtta tcgggtactg
     1381 cagcttggaa tttttacact atagttcaat atattttagg tgtaaaaccc gattataacg
     1441 gtatttcaat aaatccatgt attccaaaaa cttgggatgg tttttctatg aaacgtgtat
     1501 tccgtggtgc tacatacgta attgaagttt ctaatcctaa tcacgtaagt aaaggagtaa
     1561 aacaaataat tgtaaatggt aatactattg aaggtacaat tgtacctatt ttagaagccg
     1621 gaaaaacaca taccgttcaa gttataatgg gataatatac cctctaataa aagtcaaaaa
     1681 aacgttatag gatttatttg actaataatt aatataaaaa aaagctgtct gatttttaat
     1741 ataatcagac agcttttttt atgaatacaa tattttattg caaacttttt tacctgtgca
     1801 tcttttcaac aacaatatgt taaaaaaata atagaattta atattgttta gtgtataaat
     1861 taatttagtt ttgttttatt acaatttaca ggttcctttc caaaaatata ttggaattgt
     1921 ggattaaaag ggaatcccgt gaaaaacggg agctatcccc gtagctgtaa gtccttttat
     1981 acgttttggc accccttgcc actgaccgcc gcaggcggtt gggaaggcag ccaaaatagg
     2041 acaagccaga agacctgcct gtaaaataat tcgtagcttt cgggtgaaaa gcgaagagta
     2101 aagatttgta catatcaata tctttatttt tctttttttt gagagctacc atatttaatg
     2161 tttaacaact tactaaatat taaaatctat ggtaactaaa gacgtattaa aaaacagaca
     2221 aatcagtaaa cgagatggac gtattgtccc tttcgaagca gaaaaaatta cttttgctat
     2281 ttttaaagca ttgcgagcag ttggccgtcc caaccgtgaa cttgccgaag atctcatgct
     2341 cgatgtacta aaacaattgt gtatgttcga taaaccgaat tacacacctt ctgtagaaga
     2401 agttcaagac acagttgaaa aagcgttgtt tgagaataaa ctttttgatg ttgctaaagc
     2461 atatattcta tatcgcaagc aacacgaaca tattcgtaat gctaaagaac ttttttctaa
     2521 tcttgacatt gtagaaaaat acctgaatct cgacgattgg cgcataaaag aaagtgcaaa
     2581 ttcatcgtat tcattacaag gtcttaatca acatatagcc accattatta gctctcagta
     2641 ttggttagag cgcatctatt ccgatgaagt caaacaggcc tatcaaacag gtcgttttca
     2701 tatacacgat ttaggctttt tgagtgttta ctgtgtcggt tgggatttag aagatttgtt
     2761 aattaccgga tttaaaggag taagcggcaa aatagaaagt cggccggcca aacatttgcg
     2821 gactatatta ggacaaatag tgaacttttt ttataccatg caaggcgaag ctgccggtgc
     2881 acaagctttt tcaaattttg atacctacct tgctcctttt atcaggtacg ataaactcaa
     2941 ttatgcacaa gtaaaacaag ctttacaaga atttatgttc aatataaatg ttcccactcg
     3001 tgtgggcttt caaacaccgt ttaccaatat tacactcgac cttaaaatac ctgaattttt
     3061 aaagaatcag cctgtaataa ttggcggaaa acctcaagaa acagtttacg gcgattttcg
     3121 ggctgaaatg gatatattta atgctgcgtt tgccgaagta atggccgagg gcgatgctaa
     3181 cggaagtgta ttttcattcc ctatacctac ctataacatt accaaagatt tcgaatggga
     3241 taatcctgca tacaccaaaa tatgggaaat agcagcaaaa tacggtattc catatttttc
     3301 aaattttgta aactctgaca tgaatccaga cgatgtacgc agcatgtgtt gtcggttacg
     3361 tttagataaa agagaactaa aaaaacgtgg cggaggctta tttggtgcta atcctcttac
     3421 cggtagtgtt ggggtggtta ccataaattt accacggtta ggttacgaat caaaatcaga
     3481 aaccgagttt tttcaacgcc tcgataaact aatggaatta gcaaaagaaa gccttgaagt
     3541 aaaacgaaaa gtaatagaaa accttaccga aagaggtctt tatccatatt caaaatttta
     3601 tttgcgccat gtaaaacaac gtacaggcaa attttggaaa aaccactttt caaccattgg
     3661 tattgttggc atgaacgaat gttgtctcaa ttttttaggc tgcaaccttg tttctgaaaa
     3721 aggatatgct tttggattaa aaataatgga cacaatgcgt ttaaaactcg aaatgttcca
     3781 agatgaaaca ggcaatatat acaacctcga
//
LOCUS       DAOTOW010000128         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19234_length_3807_cov_3.301972, whole genome shotgun sequence.
ACCESSION   DAOTOW010000128 DAOTOW010000000
VERSION     DAOTOW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19234_length_3807_cov_3.301972"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="P5243_03205"
     CDS             <1..1216
                     /locus_tag="P5243_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18489.1"
                     /translation="LSQPFIFKIVSTLPADINTHFKLTYASANYYGYEYIPVSFNPSY
                     YDFEIGNFKATATANSSIGVMNPTNKQENGIVYKNFPNLLYQGGLVLAQNDTLISAQF
                     RTPRNFSIVQFPKILQADSVDLLIQSKYKSNNLKLDITQYIYAWDDIDALIHEYRITN
                     KTDSTIHNLKAGAFIDWQIISQFYNKLSYTDSLKLAHITTVDPLGFHAGIMSLHHHTS
                     SIYAVDDAQGLDSVYISDAFTDPEIWFTLNREKKQAGYLQYEGAPIASVSYCTIGELP
                     KDSTVKIRYAIIAGESKQDIVDVARILKQKYVIDTTKAPIINIAEPLQYGTIISLVTN
                     SCTIQYEQNQTVLTIALYDMQGKKIYTHSIQPQDQTGIYTCHFPNIVPGIYTIKLKQG
                     SVTSTCKIISKQ"
     gene            1240..2151
                     /locus_tag="P5243_03210"
     CDS             1240..2151
                     /locus_tag="P5243_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18490.1"
                     /translation="MKHFITILCSFIIIQCGFAQSSRQLIHAENTLISIYSKISRFAS
                     DSSNKANNTQFISLLDSVLQLPESFLYPFNDLKCGNVYAPDKTFRLITWNVIHSSGTY
                     ENFGFLQTYNKQTKKVKVYKLTDKSTTIKTPMLAICSPDVWYGASYYMVCQKKYKGQI
                     LYTLIGWNPNNTFTQKKVIETLKFDKSGNPVFGFPVIELKGKGFQRRIIFEYSSKNTM
                     MLRYDEKKDMIVFDHLAPSDFRYDGIYEFYGPDFSIDAYTFDRGKWRYVSDIDIRNPR
                     QGIKDYIPPRIKALFKKKQSKENPLGL"
     gene            2174..2584
                     /locus_tag="P5243_03215"
     CDS             2174..2584
                     /locus_tag="P5243_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18491.1"
                     /translation="MKYTSLFTITFIIGLFACNSKQPINNENIQGTWTCFDYTISGEG
                     INQTMETQSKQIALQTTYKFIHDTLIMKNDYFILPSTYSISADGKQMICSVIGYTNIQ
                     PRIFSIQDFTGDVLVLEEAQQGITTKTFLQRNKD"
     gene            complement(2581..3396)
                     /gene="zupT"
                     /locus_tag="P5243_03220"
     CDS             complement(2581..3396)
                     /gene="zupT"
                     /locus_tag="P5243_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRS18492.1"
                     /translation="MENTVLFAFLLTLFAGLSTGIGSVMALFSKQFNPKFLALSLGFS
                     AGVMIYVAFVEIFSKARHALTESFGNEVVLGLNKAYLLTVVSFFAGIAIIALIDKFVP
                     SAENPHEIKSLENTQNVKDKKLMRLGIFSALAIGIHNFPEGLATFIAALQDPTLGISI
                     AFAIAIHNIPEGIAVSVPIYYATKNKKRAFTLSFLSGLAEPIGAIIGYLLLRTVLDQS
                     VLGYVFASVAGIMVYISIDELLPTAEEYGEHHIAIWGLIAGMFTMAMSLVLFA"
     gene            complement(3402..>3807)
                     /gene="thrH"
                     /locus_tag="P5243_03225"
     CDS             complement(3402..>3807)
                     /gene="thrH"
                     /locus_tag="P5243_03225"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HRS18493.1"
                     /translation="PLPGALEFTKWLRSVTRFVIVSDTFVEFAQPLMKQLEYPFLLCH
                     SLEIDEAGRVINYKIRQPDPKRKTVEAFQSLQYKVIAFGDSYNDISMLKAADIGVLYC
                     PPQNVINDYPELPVARNYEELTSILHAHIFQD"
BASE COUNT         1439 a          636 c          547 g         1185 t
ORIGIN      
        1 actttctcaa ccatttatat ttaaaatagt aagcacattg cctgccgata taaatactca
       61 ctttaaactt acctatgctt ctgctaatta ttacgggtat gaatatattc ctgtatcatt
      121 taatccttcg tattatgatt ttgaaatagg gaattttaaa gcaactgcaa ctgcaaacag
      181 ttcaattgga gtaatgaatc ctactaataa acaagaaaat ggtattgtat acaaaaattt
      241 tcctaatcta ttatatcaag gaggattagt tctcgctcaa aatgatactc tgatttctgc
      301 acaatttaga actcctcgca atttttctat tgtgcaattt cccaaaatat tacaagccga
      361 tagtgttgat ttattaatac aaagtaaata taaaagtaat aatttaaaat tagatataac
      421 tcaatatatc tatgcttggg acgatataga tgctctaata catgaatacc gaataacaaa
      481 taaaacagat tctacaattc ataacctaaa agcaggtgct tttattgatt ggcaaattat
      541 cagtcaattt tataacaaac tatcttatac cgatagcctt aaacttgctc atattacaac
      601 agttgatcct cttggcttcc atgcgggaat tatgtcatta catcatcata catcaagtat
      661 atatgctgtt gatgatgctc agggtttaga tagcgtgtat ataagtgatg cttttaccga
      721 tcctgaaata tggtttactt tgaatcgcga aaaaaaacaa gccggatatt tacaatacga
      781 aggagcacct atagcatcag tttcatactg tacaattgga gaactaccta aagattcaac
      841 agtaaaaatt cgttatgcaa taatagcagg tgaatcaaaa caagatatag ttgatgtagc
      901 acgcatatta aaacaaaaat atgttattga cacaaccaaa gctcctataa ttaatattgc
      961 tgaaccttta caatatggca ctattatatc attagttacc aattcttgta ctattcaata
     1021 tgaacaaaat caaacagtat tgactattgc tctgtatgat atgcaaggaa aaaaaatata
     1081 tactcacagt attcaaccac aagaccaaac aggaatatac acatgtcatt tccctaatat
     1141 agtacccgga atatatacta taaagctaaa acaaggatcg gtaacgtcta catgtaaaat
     1201 aattagtaag caatagcgtt gtattttgaa aaattaacga tgaaacattt tattacaatt
     1261 ctatgtagtt ttataattat tcaatgtggg tttgctcaat catcacggca attaatacat
     1321 gcagaaaata cgttaatcag catttactca aaaatatcac gatttgcttc tgattcttca
     1381 aataaagcaa ataatacaca atttatcagt ttacttgatt ctgtattgca attacccgaa
     1441 tcatttctat atccgtttaa tgaccttaaa tgtggcaatg tatatgcccc cgacaaaaca
     1501 tttcgattaa ttacttggaa tgttattcat tctagtggta catatgaaaa ttttggtttt
     1561 ttacaaacat acaataaaca aactaaaaaa gtaaaagtat ataaattaac tgataaatct
     1621 acaacaatta aaactcccat gctcgccatt tgtagtcccg atgtatggta tggagcatca
     1681 tattatatgg tgtgtcaaaa aaaatacaaa ggtcaaatat tatatacact cataggttgg
     1741 aatccaaaca atacatttac tcaaaaaaaa gtaattgaaa cactcaaatt tgacaaatca
     1801 gggaatccgg tatttggatt tccggtaata gaattgaaag gaaaaggatt tcaaagacga
     1861 attatttttg aatattcttc aaaaaatact atgatgttgc gatatgatga aaaaaaagat
     1921 atgattgtgt ttgaccattt ggccccttca gatttcagat acgatggaat atatgaattc
     1981 tatggccctg atttttctat tgacgcatac acttttgatc gtggaaaatg gcgatacgtt
     2041 agcgatattg atatccgtaa tccacgtcaa ggaataaaag actatattcc acctcgaata
     2101 aaagcactgt ttaaaaagaa gcaatctaaa gaaaaccctt taggattata aatacattca
     2161 atacttatac actatgaaat atacttcact tttcacaata acttttataa ttggactatt
     2221 tgcgtgtaat tctaaacaac ctataaataa tgaaaatata caaggtacgt ggacatgttt
     2281 tgactataca atatcaggag aaggtataaa tcaaactatg gaaactcagt ctaaacaaat
     2341 tgcattacaa actacatata aatttataca tgatacgctt atcatgaaaa atgattattt
     2401 catattacct tctacttact caatatctgc cgatggtaaa caaatgattt gtagcgtaat
     2461 aggctacaca aatatacaac cacgtatatt ttcaatacaa gattttacag gagatgtact
     2521 tgtactagaa gaagcacaac aaggaattac aactaaaact ttcttacaaa gaaataaaga
     2581 ttaagcaaat aataccagac tcatagccat agtaaacata ccggcaataa gtccccatat
     2641 tgctatatga tgctcaccat attcttcggc tgtaggcaat aattcatcaa tagaaatata
     2701 aaccataata ccggcaaccg aagcaaaaac atatcccaaa acagattgat ctaatactgt
     2761 tcgcaacaaa aggtagccta taatagctcc tataggttca gctaaacccg ataaaaatga
     2821 taaggtaaat gctctttttt tatttttagt agcataataa ataggaacag aaacagcaat
     2881 accttcggga atattatgaa ttgcaattgc aaatgctata ctaataccta atgtaggatc
     2941 ttgtaatgct gcaataaacg ttgccaaacc ttcgggaaaa ttgtgtattc caatagccaa
     3001 tgcagaaaaa attccaagac gcataagttt tttatctttt acattttgag tattttcaag
     3061 cgatttaatc tcatggggat tttctgcaga tggtacaaat ttatcgataa gtgcaataat
     3121 agctataccg gcaaaaaatg atacaacagt aagtaaatac gctttgttta aacccaatac
     3181 aacttcatta ccgaaacttt ctgtaagagc atgacgtgct tttgaaaaaa tttcaacaaa
     3241 agcaacatat atcattactc ctgccgaaaa acctaatgaa agagctaaga attttggatt
     3301 aaattgcttt gaaaacaaag ccattacact acctataccg gtagataacc ctgcaaataa
     3361 ggttaataaa aatgcaaata atacagtatt ttccattcgt attaatcttg aaaaatatgt
     3421 gcatgtaata tagaagtaag ttcctcataa ttacgagcta caggtaattc aggatagtcg
     3481 tttattacat tttgcggtgg acaatacaaa actccgatat cggcagcttt tagcattgat
     3541 atatcattat aggaatcacc aaatgctata actttatatt gtaaactttg aaatgcttca
     3601 acagtttttc gtttaggatc gggctgtcga attttataat taatcacacg acccgcttca
     3661 tctatttcta aagaatgaca caacaaaaat ggatattcca attgtttcat gagtggttga
     3721 gcaaattcta caaatgtatc agatacaatt acaaaacgag ttaccgaacg taaccatttg
     3781 gtgaattcta atgcgccggg taagggt
//
LOCUS       DAOTOW010000129         3766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19430_length_3766_cov_2.905147, whole genome shotgun sequence.
ACCESSION   DAOTOW010000129 DAOTOW010000000
VERSION     DAOTOW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19430_length_3766_cov_2.905147"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2089
                     /locus_tag="P5243_03230"
     CDS             <1..2089
                     /locus_tag="P5243_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS18494.1"
                     /translation="YPQAITWYKRADDVKYKDETKTMYFNYGNMLMMAGEYAAAKKMY
                     KTHLTRNSKDEYAKMKMKSCDFADTAKSLENIYEYRNVQELNTNFNEFSPAACKEKVI
                     FASSRKTPDGYTYNVTGEGFEDLYETFHNEKSNIWAPPIRLQGINTSYNDGTFSFDPK
                     RNIAYIMQCNGAKGIEKNCNIYTSDYNEATNMWSKPKKFEHFSTAYSSGHPSITPDGN
                     TLYFASNNPAGLGGTDIWVIKRDPQTDKWGKPENLGKPINTPFDEMFPYAFDTKGLYF
                     SSNGHMGFGGLDIFYSEFIDTSFSKPVNLKAPFNSSADDFGLLLLSDNSGFFTSSRPG
                     GVGGDDLYYFEYKKVEIVAAGRVTDKDTYQPIPNAIVVIQNNTKQTSDTLITDEFGVY
                     SYQFLEKDMDYTIITFKDEFLNPESKWVSTMGITQNTYMDSLHGYNLNFTMQKIVAGK
                     EYIIRDIFYDLDKYSLRPESITELNNIVNILRNNPEICIQINSHTDNRASDEYNITLS
                     HNRAKSVVDYLISQGISDKRLAWQGWGETRPLVPNAQTEEEHQMNRRTSFTIVNVEQL
                     HLGEKAKFHNQLAQRVAQSQGNEEPVEQGVYYRIQISATRSACNAQTFAKIEKQFPGI
                     PTYCTKYPDGFYRYTVGLYRNLDEVEGAKTKIDQLGYNSFIVAYNNGQKISINQAIKL
                     TKPKDDSAIEITD"
     gene            2100..3023
                     /locus_tag="P5243_03235"
     CDS             2100..3023
                     /locus_tag="P5243_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRS18495.1"
                     /translation="MAKIYSYILVILLIGVTHSLTIAQTDIQQTNYMFNELSFNPAYT
                     GSTNNLRATVLARKQWFGFDHSPLTQTLCVDNATRFGGIGLNIINDQVGYERSLYAKL
                     LYSYTAQFSDNTALTAGFAAGVIHRMLDGSQLEFQDETTTDPAAILDMQSGLKPTIDF
                     GLQFLHKDFAIGVSSAHIQTSLQTATIFNMPRHFYGYAQYRIKAGEKLAFVPTVYAKS
                     GSNVYQVEANTNIYFNKKFWLGASYRLDESMVALAGIIIKEQLYIGYAYDFNVGDVSP
                     YSYGSHEIFISFRKKPPTPQSGFYQSTRLFN"
     gene            3298..3369
                     /locus_tag="P5243_03240"
     tRNA            3298..3369
                     /locus_tag="P5243_03240"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3331..3333,aa:Glu,seq:ttc)
     gene            3391..3645
                     /gene="rpsT"
                     /locus_tag="P5243_03245"
     CDS             3391..3645
                     /gene="rpsT"
                     /locus_tag="P5243_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRS18496.1"
                     /translation="MANHKSSIKRIRQTETKRVHNKYYAKTTRNAVKKIQTSTDAAEA
                     KEMLPKVTAMLDKLAKKNIIHKNKASNLKSSIAKHVNSLA"
BASE COUNT         1408 a          551 c          640 g         1167 t
ORIGIN      
        1 atatccgcaa gctattacgt ggtataaacg tgccgatgat gtaaaataca aagatgaaac
       61 aaaaacaatg tattttaatt atggtaatat gcttatgatg gccggagaat atgctgctgc
      121 taaaaaaatg tataaaaccc atcttacccg aaattcaaaa gatgaatatg ctaaaatgaa
      181 aatgaaatca tgcgattttg ctgatactgc aaaaagttta gagaatattt atgaatatag
      241 aaatgtgcag gagttaaata caaatttcaa tgaattttca cctgcagctt gtaaagaaaa
      301 agtcatattt gcatcgtcgc gtaaaactcc cgatggttat acgtataatg ttacaggtga
      361 ggggtttgaa gatttgtacg aaacatttca taatgaaaaa tcaaatattt gggcacctcc
      421 tatacgtttg cagggaatta atacaagtta taacgatggc acattttctt ttgaccccaa
      481 aaggaatata gcatatatta tgcaatgtaa tggagcaaag ggtattgaga agaattgtaa
      541 tatttatact tcagattata atgaagcaac taacatgtgg tcaaaaccaa agaaatttga
      601 acatttttct acggcatata gttcaggaca tccttcaatt actcccgatg gtaacacatt
      661 atattttgcc tcaaataatc ctgctggatt gggaggaact gatatttggg ttataaaacg
      721 tgatccgcaa actgataaat ggggcaaacc cgaaaatttg ggaaaaccta taaatactcc
      781 ttttgatgaa atgtttccct atgcttttga tactaaaggg ctatattttt catcaaacgg
      841 acatatggga tttggtggat tagatatatt ttattctgaa tttatagata cttcattttc
      901 aaaacctgta aatttaaaag ctccatttaa tagtagtgcc gatgattttg gtttattatt
      961 gctttcagat aattcaggtt tttttacatc gagtcgtccc ggtggtgttg gtggtgatga
     1021 cttatattat tttgaatata aaaaagttga aattgttgct gccggtagag ttaccgataa
     1081 agatacgtat cagccaatac ccaatgctat tgtagtaatt caaaataata ctaaacaaac
     1141 ctcagatact ttaattaccg atgaatttgg tgtgtattca tatcaatttt tagaaaaaga
     1201 tatggattat actattatta catttaaaga tgaattttta aatcctgaaa gtaaatgggt
     1261 ttctactatg ggaattactc aaaataccta tatggattca cttcatggat ataatttaaa
     1321 ttttactatg caaaaaatag ttgccggtaa agaatatatt atacgcgata ttttttatga
     1381 tttagataaa tacagccttc gtcctgaatc tattacagag ttaaataata ttgtaaatat
     1441 tttgcgtaat aatccagaaa tatgtattca aataaattca catacagaca atagagcaag
     1501 tgatgaatat aatattacct tatcacataa tagagcgaaa tctgttgtag attatcttat
     1561 ttcacaagga attagtgata aacgtttggc ttggcaaggg tggggtgaaa cgcgtccgtt
     1621 agtgcccaat gcacaaacag aagaagaaca tcaaatgaac agaagaactt catttacaat
     1681 agtgaatgta gagcaacttc atttaggtga aaaggctaag tttcataatc aattggctca
     1741 acgtgttgct caatcgcaag ggaacgaaga acctgttgaa cagggggtat attatcgtat
     1801 tcaaatttcg gcaacacgaa gtgcatgtaa tgctcaaaca tttgcaaaaa tagaaaagca
     1861 atttccggga attccaacgt attgcacaaa atatcctgat ggattctatc gctatactgt
     1921 aggtttatat agaaatctag atgaagtaga aggtgcaaaa acaaaaatag atcaattagg
     1981 gtataattca tttatagtag catacaataa cggacaaaaa atatcaataa atcaagctat
     2041 taaattaaca aaaccaaaag atgatagtgc aatagaaata acagattaaa tagataatta
     2101 tggcaaaaat atattcatat atacttgtaa tattacttat aggagtaacg cattctttaa
     2161 ctatagccca aaccgatatt cagcaaacaa attatatgtt taacgaattg tcatttaacc
     2221 ctgcgtatac cggcagtaca aataatttgc gggcaactgt attggcacgt aaacaatggt
     2281 tcggatttga ccacagtcca cttacacaaa ctctgtgtgt tgacaatgct acacgctttg
     2341 gaggtattgg actcaatata ataaatgacc aagtaggata tgagcgatca ttgtatgcaa
     2401 agttgttata ctcatataca gcacaatttt ctgacaatac agctcttact gccggttttg
     2461 cagcaggggt tatacataga atgcttgatg gaagccaatt agaatttcaa gatgaaacaa
     2521 ctactgaccc tgcagcaatt cttgatatgc aatcaggttt gaaaccaact attgattttg
     2581 gtttacagtt tttacacaaa gattttgcta taggggtatc atcggcacat attcaaacat
     2641 cgttgcaaac tgcaactata tttaatatgc cacgccactt ttatggctat gctcaatata
     2701 gaataaaggc aggcgaaaaa cttgcatttg taccaacagt atatgccaaa agcggaagta
     2761 atgtatacca agtagaagca aataccaata tatactttaa caaaaaattt tggttaggag
     2821 catcatatcg tttagacgaa tctatggtag cacttgcagg cattattata aaagagcaat
     2881 tatatatagg atatgcatat gattttaatg taggtgatgt aagtccgtat agttatggtt
     2941 cgcacgaaat attcatttcg ttccgtaaaa aaccgccaac accacaatcc gggttttatc
     3001 aaagtacacg attatttaac taatgttatt gtaatataag tatatattgg attggcgttg
     3061 taataatttt acctatttga ggaatagatt tttatgaagt tataaaatct ctaaaaaaga
     3121 gatatgtgtt tcaaaatctg tgaggtaaga tgtttcaata agtttgttga atacactata
     3181 catatcaata aaataggggt ttttatgtaa aacaaataaa aaaataaaaa aaatatttga
     3241 aagctattgc aaaaataaaa atgtagtata tctttgcacc cgcattgaaa aactaacggc
     3301 tcgttcgtct aggggttagg acgccagatt ttcattctgg tagcaggggt tcgattcccc
     3361 tacgagctac aaaaaaatat aaataataat atggcaaatc ataaatcgtc aattaaaaga
     3421 atccgtcaaa cggaaacaaa acgtgttcat aataaatatt atgcgaaaac aacacgtaat
     3481 gctgttaaaa aaatacaaac atcaacagat gctgctgaag caaaagaaat gttacctaaa
     3541 gtaacagcaa tgcttgacaa acttgctaag aaaaatatta ttcataaaaa taaagcatcg
     3601 aatttaaaat catcgatagc aaaacatgtt aattctttag cataattgta tttaaaataa
     3661 aatgaaagaa aaagcctctg aattttcaga ggcttttttt gtatattttt tgtcattatt
     3721 gtccgataaa actacaaaat taatttaata aaacaaaatt tatttg
//
LOCUS       DAOTOW010000130         3759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19466_length_3759_cov_3.812365, whole genome shotgun sequence.
ACCESSION   DAOTOW010000130 DAOTOW010000000
VERSION     DAOTOW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19466_length_3759_cov_3.812365"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..2956
                     /locus_tag="P5243_03250"
     CDS             206..2956
                     /locus_tag="P5243_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS18497.1"
                     /translation="MLYDSSTDLLWCSTFGDGIVVFDCKTYTFVPENKLKELYPEWNN
                     SMFTSKILFDTVDSSVWFIDGIVLGRYKNKTVHSFYQSDSTSECTDGMFCSDILQTKS
                     GMIYIAGYSGFKYFDRNENCIKNITFPHCNFSDAKSLLEDGKGNIWVATPQALFRYDP
                     KNKTVRNISQVWGMPHIQYYRKSAFRTNDGHLYFGSLKGFIVLHEDSSYTYNITPSLH
                     ITKLYINNIEQRPGMEGSPISKDITLLKELVLSHNQSFITIEFGVLNFIDNEKSLCKY
                     KMLGFDRDWIYAGKERRATYTNIPPGTYTFLVSTTNSEGQWIETPHSLTIIILPPWWK
                     TLWFKLIVILIIVFGVFIFIHLREKAIKEINKQLEQKVDEKTKELQLANSTLLEQKNT
                     IEKHYENLREQQLVIEIKNSQLQEALNTKNKLLTVIAHDFKNPLSTLIGFAKLLQDKI
                     MTKKNSDLLPGIQSIVNSAQTIHNQMIEVLEWSVSKDDAVKYNPMDINIEILIKDTLS
                     LIQDTAAQKNILIEASFKITHAAFIDPRMMSAIIRNLVINSIKFTPRNGKVSILVSQQ
                     KTAIEITVKDTGIGMNQEQIDRILSDTYMISEDYKSGFGLQLCKTFIKRNNGTLHIYS
                     EVDKGSTFVVQVPKGSMLEKPIEKKKEVIYDSNADFDEEDSQRKMLVIDDNVEITSYL
                     RELFSDSFTVLTAKDGKEGLDKALLSLPEIILTDINMPEMDGKQLCRQLRSNSLTNHI
                     PIILISAQSQPHEQIEGLQMGADDFIEKPFNANVLKQKVYMLLRNREKLIEHVKQSLN
                     TTDKFNLPDSFDDKIIKEITDVIIENISNVDFKVDMLADRVGLSRSQLYRKTISVLGQ
                     SPNDYIKSLRLQQAVEMLKTGRYRISEIAYEVGFSDPGYFSACFFEKYGIKPSDYGKM
                     KE"
     gene            complement(3360..3599)
                     /locus_tag="P5243_03255"
     CDS             complement(3360..3599)
                     /locus_tag="P5243_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18498.1"
                     /translation="MKQNLINWVENNYKKWIGLENGITIKELLSIVPDEILNFYNSST
                     KKEIIKICYDLGYKFIEVNKRLNGKVGRYRYLIPK"
BASE COUNT         1332 a          534 c          646 g         1247 t
ORIGIN      
        1 ttaaaatcaa gaatttacca ataatagtat aaaagttgta ttagattatg attaaattct
       61 tgatataaaa gaaataaaac ccggcattct tgcaattgca tcatggaatg gagctcttaa
      121 tatatttaac gagaaaacag gtacttttac caaatacaca ttcaaatcat ctaattcaaa
      181 aacggtttca ttaaatatat acggattgct gtatgattca tctaccgact tgctttggtg
      241 tagcacgttt ggtgatggaa ttgtagtgtt tgattgtaaa acctacacgt ttgttccaga
      301 gaataaacta aaagaattat atcctgaatg gaataattca atgtttactt caaaaatttt
      361 atttgataca gttgattcat cggtatggtt tattgatgga attgtgttgg ggcgatataa
      421 aaataagacg gttcattcat tttatcaaag tgattcaaca tcagaatgta ccgatggtat
      481 gttttgcagc gatattttac agactaagtc gggaatgata tatatagcag gatattccgg
      541 gtttaagtat tttgatagaa atgaaaattg tattaaaaat ataacatttc cacattgtaa
      601 tttttctgat gcaaaatcat tacttgaaga tggtaaaggc aatatttggg ttgcaacgcc
      661 gcaagctttg ttccgatacg atcctaaaaa taaaacagtt agaaatatat cacaagtatg
      721 gggtatgccg catattcaat attatagaaa atctgcattt agaaccaacg atggccattt
      781 atattttggt agtttaaagg gatttattgt tttacacgaa gatagttcat atacatataa
      841 tattaccccc tctcttcata ttactaaatt atatataaat aatatagagc aacgtcccgg
      901 catggaaggt agtcctatat ctaaagatat tacattactt aaagaattag ttttgtctca
      961 taatcagtcg tttattacaa ttgaatttgg ggttttgaat tttattgata atgaaaaatc
     1021 tttgtgtaaa tataaaatgc ttgggtttga cagagattgg atttacgcag gtaaagaacg
     1081 acgagctacc tatactaata ttcctccggg cacctatact ttccttgttt ctactacaaa
     1141 tagtgagggg caatggattg aaacacctca ttctttaacc ataattattt tgccgccttg
     1201 gtggaaaact ttgtggttta aattgattgt aattctgatt attgtatttg gggtgttcat
     1261 atttattcat ttacgtgaaa aagctattaa agaaattaat aagcaattag aacaaaaagt
     1321 tgatgaaaaa accaaagaat tgcaattggc caatagtact ctgctcgaac aaaaaaatac
     1381 aattgaaaaa cattacgaaa atttacgaga gcaacaattg gttattgaaa ttaaaaattc
     1441 gcaattgcaa gaagctctta atacaaaaaa taaattactt accgtaattg ctcacgattt
     1501 caaaaatcca ttgtctacgc ttataggttt tgctaaattg ttgcaagata aaattatgac
     1561 taagaaaaat tccgatttat tgcctggaat tcaatctata gtaaattctg ctcaaacaat
     1621 tcataatcaa atgatagagg tgcttgaatg gagcgtgagt aaagacgatg cggtgaagta
     1681 taatcctatg gatataaata tagaaatact tattaaagat actctttcgc ttatacaaga
     1741 tactgctgca cagaaaaata tattgataga agcttcgttc aaaattactc atgcagcttt
     1801 tatagaccct cgtatgatga gtgcaattat tcgcaatttg gtaatcaata gtattaaatt
     1861 tacacctcga aacggaaagg tatctattct ggtaagtcaa caaaaaacgg caatagaaat
     1921 tactgttaaa gatacaggta taggaatgaa tcaagaacaa atagatagaa ttttgtctga
     1981 tacttatatg atttccgaag attataaaag cggatttggt ttgcagttgt gtaaaacatt
     2041 tataaaacgc aacaatggaa cgctccacat atatagcgag gtcgataaag gttctacctt
     2101 tgttgtccaa gttcccaaag gaagtatgct cgaaaaacct attgaaaaga aaaaagaggt
     2161 aatatatgat tctaatgccg attttgacga agaagattcg caacgtaaaa tgttggtaat
     2221 tgatgataat gttgaaatta catcatatct tcgtgaatta ttctcggatt cgtttacggt
     2281 gcttactgcc aaagatggaa aagagggatt agataaagca ttactttcgt tacccgaaat
     2341 tatattgacc gatataaata tgcccgaaat ggatggtaag caattgtgcc gccagttacg
     2401 aagcaatagt ttgaccaatc atatacctat tatacttatc agtgcccaat cgcagccaca
     2461 cgaacaaata gaaggcttac aaatgggagc cgacgatttt atagaaaaac cattcaatgc
     2521 taatgtgttg aagcaaaaag tatatatgct gttgcgtaat cgcgagaaac tcatagaaca
     2581 tgtaaaacaa tcgctcaata ctaccgataa attcaatttg cctgatagtt ttgacgataa
     2641 aattataaaa gaaataaccg atgtaattat tgaaaatata agcaatgtag actttaaagt
     2701 agatatgctt gccgataggg taggtttgag tcgttcgcaa ttgtaccgta aaactatatc
     2761 ggtattggga caatcgccaa acgactatat taaaagtctc cgcctgcagc aagcagtaga
     2821 aatgctaaaa accggcagat accgaatttc ggaaattgct tacgaagttg gcttctcaga
     2881 cccgggctac tttagtgctt gctttttcga aaaatatggt ataaaaccat cggattatgg
     2941 gaaaatgaag gaataatatg ttaattttta atttttaatt cttaattttt aattgaaaat
     3001 acagtgatat aatcccgaag gcttattgaa aataatccac ttttttgtgt cttagtgact
     3061 ttgtggcaat tttaataaag ttggggttac ggtatagggt ataaggttta gaggtttaag
     3121 ggtatatggg tgtataggta atttaattat gaattaatca ttatcaatta tcaattattt
     3181 taaccccgaa ggggtgtaat tattatagaa actacaggta caacaatcat aaaccccgaa
     3241 ggggtgatat taaagagatg taagcaaaag gtttacggta tagggttagg attttttatt
     3301 gaaaacatgg taaaatgccc ccaaagaatt gatattcacg cgtgaatgtg tgaaaaattt
     3361 tattttggaa tcaaatatct atatctacca accttcccat ttagtctttt atttacttca
     3421 ataaatttat atcctaaatc ataacaaatc ttaataattt ctttttttgt tgatgaatta
     3481 taaaaattta atatttcgtc tggaactata gatagcaatt ctttgattgt tattccattt
     3541 tcaagtccta tccatttttt atagttgttt tcgacccaat taataagatt ttgcttcatt
     3601 tcatcgcgag gatatataat actccctatt agtaggacag tttagttatc ttttttagat
     3661 ataattttca ttatttatta tccaaattta catttttatt tccggtttgt ttcgaacggg
     3721 aaataacttt ttgcccacta aaaggcaaaa aaagttatt
//
LOCUS       DAOTOW010000131         3750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19513_length_3750_cov_3.162382, whole genome shotgun sequence.
ACCESSION   DAOTOW010000131 DAOTOW010000000
VERSION     DAOTOW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19513_length_3750_cov_3.162382"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="P5243_03260"
     CDS             <1..599
                     /locus_tag="P5243_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HRS18499.1"
                     /translation="NAGSSINSGLSTAQALTVTQIAAAAGAVTWLLIEGFHQGKASAL
                     GLASGVLAGLVAITPAAGVVTPIGALALGFFASVICYGALILKNKLGYDDTLDAFGIH
                     GVGGIVGAIFLTFFIRASWFDGMESYFFTGNRTMAEQLGIQSLAVGIAIVYSIISTLI
                     VLLVVDKTIGLRSTKEEEMKGLDDSYHGERGYGMVNPQ"
     gene            634..975
                     /locus_tag="P5243_03265"
     CDS             634..975
                     /locus_tag="P5243_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRS18500.1"
                     /translation="MKLITAIIRETQLNQVHEELIHAEIERITVSRVSGHGRQKREEI
                     YRGQVVIPSLIPKIKIEIAVNDAFVDQTIDAIIRGAKTEGDNVGDGKIFVTNLEQCIR
                     IRTSERGSEAI"
     gene            1155..2645
                     /locus_tag="P5243_03270"
     CDS             1155..2645
                     /locus_tag="P5243_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS18501.1"
                     /translation="MEQKSSSTGFQKFSWAFGGLAENLSMNVIPSLVYNIFQIGMGIS
                     PFVIGVAMSASKFIEGFSDTYFGNLSDNTRSKYGRRRPWIFVGAILLSLVFIAVWFTP
                     RQVNDMQIMGMALSGKFLQSAYFIGIISIFYIVLAIWQMPFSALGLEMETDYTQRTKL
                     QTYKLIFSYVIGVAIGSLYLITQMKEVWGGDEVVGARHVGVIVGIIILFAGIVPALFC
                     REHPNAEIQKQEKVPFWPSLIETFKSKPFILLMMSIFFIFVALFFMLPLLQYISMYHV
                     CTDGIHTVLDWSYKDPLVFSIKEKFLTHKELAGYLGFYTAVLQPTTQIITVIFVNRFA
                     GYFDKRTILISGATVTILGYVSSWFLFTPTAHVLGVLPPVIINIGLAACWPLIGSFTA
                     DICDYDELKTGARREGMYSAVCGFLIKLAIALVTIIASAVLIWVGIDGANPVISADKI
                     MDIRIMYVVIPTIAMLGTIFFIWKYPLTKAKVFEIQEQLAVKRQSK"
     gene            2671..3399
                     /locus_tag="P5243_03275"
     CDS             2671..3399
                     /locus_tag="P5243_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HRS18502.1"
                     /translation="MKTIISYTSIALLGLSIIACAPKKDKSKEIANNVTHITLEQVIN
                     KTVQYNTAYDIDSNVYKTVTIGKYEWFAQNLRVTKLNDGTPIVNVSDNAEWKKTALPA
                     YASYNNETVNEVNGLLYNYKTVESKKICPEGWQVADDSVWTNLTKTLNGEEKAAIALK
                     STNVWGDEQEATNETGFSAVPSGFRERDGKFYIIGQNALWWSATEHNEKNGMYYYIDK
                     NISLNHSHIERTVGLSIRCVKEIK"
     gene            complement(3486..>3750)
                     /locus_tag="P5243_03280"
     CDS             complement(3486..>3750)
                     /locus_tag="P5243_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRS18503.1"
                     /translation="EKSDFSVTIENEMLTIAVEKKEEKQEEKGQVTRKEFAYSSFTRR
                     FNIPQELVDAEKVEAHYDKGILTLTIPKREEVKPKPARMIEIH"
BASE COUNT         1232 a          585 c          673 g         1260 t
ORIGIN      
        1 tcaacgccgg aagtagtata aacagtggat taagtacagc acaagcactt actgtaactc
       61 aaatagctgc agcagccggt gcagttacat ggttattaat tgaaggattt catcaaggca
      121 aagcatctgc tcttggttta gcatcgggag ttttagcagg tttagtagca ataacacctg
      181 ctgccggagt tgttactcct attggtgcat tagctttagg attttttgca tcggttatct
      241 gttatggtgc attaattctt aaaaacaaat taggatatga tgacacatta gacgcatttg
      301 gaattcacgg tgttggagga attgttggtg caatattttt aacattcttt attcgtgctt
      361 catggttcga tggaatggaa agctatttct ttacaggtaa cagaactatg gcagaacaat
      421 tgggtattca aagtcttgca gtaggtattg caatagtata ttcaattatt tcaactttaa
      481 ttgtactttt ggtagttgac aaaactatag gattacgttc aacaaaagaa gaagaaatga
      541 aaggattaga cgatagttac catggtgaac gcggttatgg aatggtaaac ccacaataat
      601 aacaacaatg tataattata aaaagatatt cgtatgaaat taataacagc aataattcgt
      661 gaaactcagt taaatcaagt acacgaagaa cttatacatg ccgaaataga aagaattacc
      721 gtaagtcgtg tttcaggaca tggacgtcaa aaacgcgaag aaatttatcg cggtcaagta
      781 gtaattccat cattgattcc caaaattaag attgaaatag ccgtaaacga cgcatttgtt
      841 gatcaaacta tagatgccat aattcgcgga gcaaaaaccg aaggcgataa cgtaggtgac
      901 ggtaaaatat ttgttaccaa tttagaacaa tgtatacgaa ttcgcacaag tgaaagagga
      961 agcgaagcaa tttaatacat ttcatttcaa acatagtttt attttttcat tacaaaggct
     1021 gtttcttgga aacagccttt gtttttttag aataagacta tagtataata taaataattt
     1081 ttcgtatgtt tgccataatt taattaaact gaattttatt tttacaatag taatttacat
     1141 aacacactaa attaatggaa caaaaatcat catctacagg ctttcagaag ttttcgtggg
     1201 cattcggtgg attagcagaa aatctgtcaa tgaacgttat cccatcgctt gtgtacaaca
     1261 tttttcaaat tggtatggga attagccctt ttgtaattgg ggttgccatg agtgcttcaa
     1321 aatttattga aggtttttct gatacttatt ttggtaattt aagtgacaat acacgttcaa
     1381 aatacggtcg ccgtcgtcca tggatttttg ttggtgctat tttactttca ttagtattca
     1441 tagcagtgtg gtttacacct cgtcaagtaa acgacatgca aattatgggc atggcattat
     1501 caggtaaatt tctgcaatct gcttatttta taggcatcat ttcaatattt tatatcgttc
     1561 ttgctatttg gcaaatgcct ttttctgcat tggggttaga aatggaaacc gactatacgc
     1621 aacgaacaaa acttcaaaca tataaactta tattttctta tgtgattggt gtagctatag
     1681 ggtcgcttta tttaattact caaatgaaag aagtatgggg cggtgatgaa gttgtgggag
     1741 ctcgtcatgt tggtgttata gttggtatta ttatattgtt tgcaggtata gttcctgcat
     1801 tattttgcag agaacatcct aatgccgaaa tacaaaaaca agaaaaagta ccattttggc
     1861 catcacttat tgaaacattc aaatcaaaac ctttcatttt gttgatgatg tctattttct
     1921 tcatatttgt tgcattattc tttatgctcc ctctgttaca atatatttca atgtatcatg
     1981 tgtgtaccga tggtatacat accgtacttg actggtcata taaagatcct ttagtatttt
     2041 ctattaaaga aaaattttta acacataaag aattggcagg atatttaggc ttttatactg
     2101 ctgttttaca gcccacaaca caaattatta cagtcatttt tgtaaaccga tttgccgggt
     2161 attttgataa acgtactatt ttaatttcag gtgccacagt tactatttta ggttatgttt
     2221 caagttggtt cttatttact cctacagctc acgtattggg tgtattgcct ccagttatca
     2281 taaatatagg tttagctgcg tgttggcctc ttattggatc atttactgcc gatatttgcg
     2341 actacgatga attaaaaacc ggtgctcgac gcgaaggtat gtattctgct gtatgcggat
     2401 ttttgattaa acttgctata gcattggtta caattattgc ttctgcagta ttaatttggg
     2461 ttggtataga cggtgcaaac cctgtgattt ctgccgacaa aattatggat attagaatta
     2521 tgtatgttgt aatacctact attgcaatgc ttggaactat attctttatt tggaaatatc
     2581 cattaacaaa agcaaaagtg ttcgaaattc aagaacaatt agctgtaaaa cgtcaatcga
     2641 aataagtatg aattttaaac aatgttacga atgaaaacga ttatatcata tacatcaatt
     2701 gctcttttag gattatcaat aattgcttgt gctcctaaaa aagataaatc aaaagaaatt
     2761 gcaaacaatg taacacatat tacattagag caagttatca ataaaacggt acaatataat
     2821 acagcatatg acattgatag caatgtttac aaaacagtta ctataggaaa atatgagtgg
     2881 tttgctcaaa atttacgcgt aacaaaactc aacgacggta cacctattgt aaatgttagc
     2941 gacaatgcag aatggaaaaa aactgcatta ccggcatacg catcatataa taacgaaact
     3001 gtaaatgaag taaatggatt attgtataat tacaaaactg ttgaatccaa aaaaatctgt
     3061 cccgaaggtt ggcaagttgc cgatgatagt gtatggacaa atcttacaaa aactttgaat
     3121 ggtgaagaaa aagctgctat agcacttaaa tctacgaacg tttgggggga tgaacaagaa
     3181 gctacaaatg aaaccggatt ttctgctgtt ccttcaggct ttagagaacg tgatggtaaa
     3241 ttttacatta ttggacaaaa tgcgttatgg tggtcggcaa ctgaacataa cgaaaaaaac
     3301 ggtatgtatt attatattga caaaaacatc agtttaaacc atagtcacat tgaacgtact
     3361 gtaggtttaa gcattcgttg tgttaaagaa attaaataat tacaccaata catataaaaa
     3421 aaaggggaac atagttgttc cccttttttt tatactcact caattacctc tattcacaca
     3481 caaaattagt gaatttcaat catacgtgct ggttttggtt ttacctcttc gcgtttagga
     3541 atagtaagtg ttaatattcc tttgtcataa tgagcctcca ctttttcagc atctactaat
     3601 tcttgcggaa tattaaacct gcgagtaaat gaagaatacg caaactcttt tctggtaact
     3661 tgtccttttt cttcttgttt ttcttctttt ttctcaactg ctatagtaag catttcattt
     3721 tcaatagtta ccgaaaaatc agatttttcg
//
LOCUS       DAOTOW010000132         3746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19542_length_3746_cov_2.646979, whole genome shotgun sequence.
ACCESSION   DAOTOW010000132 DAOTOW010000000
VERSION     DAOTOW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19542_length_3746_cov_2.646979"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="P5243_03285"
     CDS             <1..1113
                     /locus_tag="P5243_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18504.1"
                     /translation="AEESDTLKTAFLANMSHEIRTPMNGIIGFSEMLAENNLTDEKRK
                     EYAQIVVKSSKQLLALVNDILDISKIEAGEVTISKDIFNVNTLIHDIYIFFKPQAIQK
                     RLEFHEVKSLADSQSVIESDKNRLRQILVNLVSNALKFTHTGEVSFGYSVSNGSITFF
                     VKDTGIGIPKHLQKEIFERFRQAELETTKLYGGTGLGLSISQKLVELLGGTIQLESEP
                     GKGSLFSFTLPYKQEKSNDEIVSQPIGNINFNKATIVIAEDNDTNFQLLEATLSKCNL
                     TIIRAVNGQDAIDICMQNSAVQFVLMDIRMPKIDGITASAEIKKYRPDLPIVIQSAYK
                     ANEERKQAIEAGCDEYLSKPIKKHELISCLLRFIPI"
     gene            1116..1541
                     /locus_tag="P5243_03290"
     CDS             1116..1541
                     /locus_tag="P5243_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRS18505.1"
                     /translation="MQQPIPQKIYEFIHEHHVLTLATVNNSIPWCCNCFYVYNSANNS
                     FIFSSDLTTRHASEMLQNTVVAGSIVLETEVIGKIQGLQFSGQVALLEKSKLTHAKKL
                     YLQRFPYAILKKTTLWECVITYAKLTDNRLGFGTKLIWE"
     gene            1516..2535
                     /locus_tag="P5243_03295"
     CDS             1516..2535
                     /locus_tag="P5243_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS18506.1"
                     /translation="MEQNLFGNKQILVTGASGLVGVHVIMYLARLGANIRAFVRNEDS
                     KQKAYTVFKAYNCCESNTITWHFGDITNYEDVYNAMKHIDYVFHCAGYVSFNNAHMQI
                     MYDVNVLGTKHVVDAALEHGITKLCHVSSVAAIGKSRTSHIVDETCEFEYTKHTGAYH
                     ITKYCGELEVWRGIAEGLQAVIVNPSVIIGVGDWYSSSSALFGTIAKGLKFYTTGSTG
                     YVSVHDVVQCMVLLMQSNITSQRFILSAQTVDYKSLFSAIADSLHVKRPHIAIKQWQM
                     KLVAILLQLVSKNPQITLHTVNSAFSHTHYSNHKICEALSYSFSNIFDDISTVGTMYL
                     KRLQK"
     gene            complement(2548..2916)
                     /locus_tag="P5243_03300"
     CDS             complement(2548..2916)
                     /locus_tag="P5243_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HRS18507.1"
                     /translation="MEKEIKTHTLGKLRVYLNNKEKVKSKTVLRKLFPKNTYIHIIQL
                     AKQCGIMNATVYTTHAGYSNFGKISRFSYETDNNSLTICIELIDTKERLQDFFIQHTS
                     IFKDKIVVFKEVEFWDTDTL"
     gene            complement(2901..>3746)
                     /locus_tag="P5243_03305"
     CDS             complement(2901..>3746)
                     /locus_tag="P5243_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRS18508.1"
                     /translation="LEVLAIGRIKYDALLPCFIAAILADMVCAAYGIKHTIYTILFHE
                     NAHSLFPYVAYSLPLLLKVIGAAIAFGLVGYLFSELSHTIKNISNKYIPYKFLIPFIG
                     GVIIIILTYIVGTTDYLGLGVYTQDGTGTSIVNAFTSKDINHWGWLLKLLFTAITLSM
                     GFKGGEVTPLFFIGATLGNSLAILMGAPIDLMAALGFIAVFAGATNTPLACTIMGVEL
                     FGGTHILYYAVACFIAYYFSGHSGIYSSQKISVSKANLNTPTDESNSLKTIRDKKISE
                     LWKKK"
BASE COUNT         1331 a          543 c          653 g         1219 t
ORIGIN      
        1 gccgaagaaa gtgacacgct taaaacagca tttcttgcca atatgagtca tgaaatacga
       61 accccaatga atggaatcat cggtttttct gaaatgcttg ccgaaaataa tttgaccgat
      121 gaaaaacgta aagaatatgc tcaaatagta gtaaaaagca gtaaacaatt gctggcattg
      181 gtaaatgata ttttagatat ttcaaaaatt gaggcaggtg aagttactat aagtaaagat
      241 attttcaatg taaatacatt aatccatgat atatatattt tctttaaacc acaagctatt
      301 caaaaaagat tagagttcca cgaagttaaa tcacttgctg attcgcagag tgtaatagaa
      361 agtgacaaaa acagattgag gcaaatttta gttaatttag taagtaatgc attaaaattt
      421 actcatacag gtgaagtatc gtttgggtat tctgtaagta acggctctat tacttttttt
      481 gtaaaagata ccggaattgg cataccaaaa catttgcaaa aagaaatatt tgagcgattt
      541 agacaagcag agttggaaac cacaaaatta tatggtggta ccgggttagg tttatcaatt
      601 tcgcaaaaat tagtagaatt attgggaggt acaattcaac ttgaatcgga acccggtaaa
      661 ggttcattat tttcatttac attaccgtac aaacaagaaa aaagtaatga tgaaatagtg
      721 agtcaaccta tagggaatat aaactttaat aaagctacta tagtaatagc cgaagataat
      781 gatacaaatt ttcagttatt ggaagcaaca ttgtcaaaat gtaatctaac aattattcgt
      841 gctgttaatg ggcaagatgc aatagatata tgtatgcaaa atagtgcagt tcaatttgtt
      901 ttaatggata tacgaatgcc aaaaattgat ggtattactg cttctgccga aattaaaaaa
      961 tacagacccg atttacctat agtaatacaa tcggcatata aagcaaatga agaacgaaaa
     1021 caagctatag aagcaggttg tgacgaatat ttgtcaaaac ctattaaaaa acatgaatta
     1081 attagttgtt tactccgatt tataccaata taataatgca acagccaata cctcaaaaaa
     1141 tatatgaatt tatacatgaa catcatgttt taacacttgc tacagtaaac aattctatac
     1201 catggtgttg taattgtttt tatgtgtata attctgcaaa taattcattt attttttcat
     1261 cagatcttac tacccgtcat gcttcagaaa tgctacaaaa tacagtagtt gccggaagta
     1321 tagtgttaga aaccgaagta attggtaaaa ttcaaggact tcagttttca ggacaggtag
     1381 cacttttaga aaaatcaaaa ttaactcatg caaaaaaact atatttacaa cggtttccgt
     1441 atgctattct aaaaaaaact acgttgtggg aatgtgtaat aacgtatgca aaattaactg
     1501 ataatagact tggttttgga acaaaactta tttgggaata aacagatttt agtaacagga
     1561 gcgtcgggtt tggtaggcgt tcatgtaatt atgtatttag ctcgattagg agcaaatata
     1621 cgtgcgtttg ttcgtaacga agattccaaa caaaaagcat atactgtttt taaagcatat
     1681 aattgttgtg aatcaaatac tataacatgg cattttggtg atattaccaa ttatgaagat
     1741 gtttataatg caatgaaaca tattgattat gtgtttcatt gtgccggata tgtgtcattt
     1801 aacaatgcgc atatgcaaat aatgtatgat gtaaatgtat taggaacaaa acatgttgta
     1861 gacgcagcgt tggaacatgg tattacaaaa ttgtgtcatg taagttcagt tgctgctata
     1921 ggtaaatcga gaacgtcgca tatagtagat gaaacatgtg aatttgaata tactaaacat
     1981 acaggggcat atcatattac aaaatattgt ggtgaattgg aagtgtggcg aggaattgcc
     2041 gaaggattac aggcggttat agttaatcca agtgttataa ttggtgtagg cgattggtat
     2101 tcgagcagtt ctgctttgtt tggaactatt gcaaaaggtt tgaaatttta tactaccggg
     2161 agcaccggat atgtaagtgt acacgatgta gtgcaatgta tggtgttgct tatgcaaagc
     2221 aatataacat ctcaacggtt tatactttcg gcacaaactg ttgattataa atctttgttt
     2281 agtgctatag ccgattcgtt gcacgtaaaa cgtccgcata tagctattaa acaatggcaa
     2341 atgaaattgg tagcaatact attacaatta gtatcaaaaa atccacaaat aacgttacac
     2401 actgttaatt ctgctttttc gcatacacac tatagcaatc acaaaatatg cgaagcttta
     2461 tcgtattctt tttccaatat ttttgatgat atttctactg ttggaacaat gtatttaaaa
     2521 aggttacaga agtaataaat agtaatatta taatgtgtcg gtatcccaaa attctacttc
     2581 tttgaatact acaattttat ctttaaaaat agatgtgtgt tgtataaaaa aatcttgtaa
     2641 tcgttctttt gtgtcaatga gttctataca aatagtaaga ctattattat cggtttcgta
     2701 cgaaaatcgt gatattttac caaagttgct atatcccgca tgtgtagtat atacagttgc
     2761 attcataata ccacactgtt tggctaattg aattatatga atatatgtat ttttaggaaa
     2821 cagttttcgc aatactgttt ttgattttac tttttcttta ttatttaaat atacacggag
     2881 tttaccaagt gtatgtgttt ttatttcttt ttccataatt ctgagatttt tttgtcgcga
     2941 atagttttga gagaattcga ttcatcagta ggagtattca gatttgcttt tgaaacagat
     3001 attttttgag aagaatatat tccggaatga ccactgaaat agtaagcaat aaaacaagca
     3061 acggcataat acaaaatatg agtgccgcca aatagttcaa cacccataat tgtacaagcc
     3121 aatggggtat ttgtagcacc tgcaaataca gctataaatc ctaatgctgc cattaaatct
     3181 ataggtgctc ccatgagaat agcaagtgaa ttacctaatg tagctcctat aaagaacaat
     3241 ggtgtaactt cgccaccttt gaatcccata cttaaagtaa ttgcagtaaa aagtaatttg
     3301 agaagccatc cccaatgatt tatatcttta gaagtaaagg catttacaat gctggttcct
     3361 gtaccatctt gggtatatac tcccagtcct aaataatcgg tagtgccaac aatgtatgta
     3421 agtattataa tgataactcc tcctataaaa ggaattagaa atttgtatgg gatatatttg
     3481 ttgcttatat tcttaattgt atgtgataat tctgaaaata aatatcctac caagccaaat
     3541 gctatagctg ctcctataac ttttaggagc aatggtaaac tatacgcaac atatggaaac
     3601 aatgagtgtg cattttcatg gaacagaatt gtatatatag tatgtttaat accatatgca
     3661 gcacatacca tatctgctaa aattgcagct ataaagcacg ggagtaatgc atcgtatttg
     3721 attctaccta ttgccaatac ttctaa
//
LOCUS       DAOTOW010000133         3743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19563_length_3743_cov_3.161876, whole genome shotgun sequence.
ACCESSION   DAOTOW010000133 DAOTOW010000000
VERSION     DAOTOW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19563_length_3743_cov_3.161876"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="P5243_03310"
     CDS             <1..1507
                     /locus_tag="P5243_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS18509.1"
                     /translation="LMFVPLLLFTNFLGSESTNITYELSVLTIKMVIIIAFVYIGNRW
                     LIPKLLHVVALLKNQELFMLAIFMICMAVSLVTFQLGMSLAFGAFLAGLMISESEYSH
                     NAFGNLITFKDIFTSFFFISIGMMLDMSFVFDNYLIVIISVLLVLSIKTIVGAATGFI
                     LGHTFRGIVMIGFALCQVGEFSFILAKIGFKTSIIDSFYYQLFLAVSVITITVTPFIM
                     NISMSVANILLKLPLPKVLIDGLFPLQEIEIPHMTNHLVIIGKDSTALKLSIMAKHIQ
                     MPHVSVIFDPTIVRDKLKNGDVAIYGDAINLPILHKAHVESADIVIISVGSAVPTMGI
                     IESVRKLSKTAHIIVRAQHVEMVEHFYKLGADQVIPQSFELAIDLFHRVLSKRLIPLK
                     ETNRIISSIRSDYLGVYSDKDLQHQASILEELSHIEISAIKIEEHSELVGKTLQDSEL
                     RKRTGVTLLAIKRGKTVIEHPNLQFIFACNDIAFVLGSPEQVNAASEIFSV"
     gene            complement(1511..2389)
                     /gene="folD"
                     /locus_tag="P5243_03315"
     CDS             complement(1511..2389)
                     /gene="folD"
                     /locus_tag="P5243_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRS18510.1"
                     /translation="MELIDGKKVSDAIKQQIADEVTIIQSQGGKIPHLAAILVGTDGG
                     SITYVNHKVKACEQVGFASTLKTFDEDISEDALLDTIAEFNSNKDIDGFIVQLPLPKH
                     IDEQKVIEAISPDKDVDGFHPINVGKMMIGLPSFKSATPAGILELLKYYHIETAGKHC
                     VIIGRSNIVGKPLSVMLAQKGYPGDCTVTVCHSRTKHLAEECKRADILIAALGSPEFV
                     TADMVKPGAVVIDVGTTRVASNETKSGWKLKGDVKFDEVAPLCSYITPVPGGVGPMTI
                     CSLLMNTLQAARGKKK"
     gene            complement(2394..3728)
                     /gene="ffh"
                     /locus_tag="P5243_03320"
     CDS             complement(2394..3728)
                     /gene="ffh"
                     /locus_tag="P5243_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRS18511.1"
                     /translation="MFENLSERLEQSFRLLKGYGKITEINVAETLKEVRRALLDADVN
                     YKIAKQFTEDVKVKALGQNVLTAVKPGEMMTKIVHDELALLMGGTKTELNTKGSPAII
                     LMAGLQGSGKTTMSGKLAHMLKNKQGKKPLLVACDVYRPAAINQLQVLGEQIQVPVFA
                     DITSKDPVSIAKSAIKHAREYNFDCVIVDTAGRLAIDETMMTEITNIKNAINPHEILF
                     VVDSMTGQDAVNTAKEFNDRLDFSGVVLTKLDGDTRGGAALSIRSVVNKPIKFVGTGE
                     KLEAIDVFHPERMADRILGMGDIVSLVERAQEQYDAEEARKLQKKISKNQFNFEDFLS
                     QIQQIKKMGNLKDLASMIPGMGKMLKNIDIDDNAFKGIEAIIHSMTPEERANPIILNG
                     SRRKRIADGSGTSIQEVNKLIKQFDETRKMMKMMQGAKGNKMMQNLMPGLKM"
BASE COUNT         1064 a          643 c          643 g         1393 t
ORIGIN      
        1 tttaatgttt gtccctttat tgctttttac taatttttta ggaagcgaat caaccaatat
       61 aacctatgaa ttatcggtgt taaccataaa aatggttata ataatagctt ttgtgtatat
      121 aggaaacaga tggcttattc ctaaactgtt acatgtagtg gcactgctca aaaatcaaga
      181 attgtttatg cttgcaattt ttatgatttg tatggcggtt tcattggtta catttcagtt
      241 gggtatgtca cttgcttttg gtgcgttttt ggccggttta atgatttctg aatctgaata
      301 tagtcataat gcatttggta atcttataac attcaaagac atttttacca gttttttctt
      361 catatctatt ggtatgatgc tggatatgtc atttgtgttt gataattatt tgattgttat
      421 aattagtgta ttattagttt taagtattaa aactatagtt ggtgctgcta ccgggtttat
      481 tttgggacac acatttagag gaattgtcat gattggtttt gcattgtgtc aagttggtga
      541 gttttccttt attttagcaa aaattggttt caaaacatct atcattgata gtttctatta
      601 tcaattgttt ttggcagttt ctgttattac tattactgta acaccattta taatgaatat
      661 atcaatgtcg gttgccaata tattgttaaa attaccttta cccaaagtac ttatagatgg
      721 tttgtttccg cttcaagaaa ttgaaattcc tcatatgacc aatcatttgg taataattgg
      781 taaggattct acagcattga aactttctat catggctaaa catattcaaa tgcctcatgt
      841 gtcggttata tttgatccta ctattgttcg cgataaactt aaaaatggtg atgttgctat
      901 atatggcgat gctattaatt tacctatttt acataaagct catgtagaat ctgctgatat
      961 tgtaataata tcagttggga gcgcagtacc aactatggga attatagaaa gtgtacgtaa
     1021 attaagtaaa acagctcata ttatagtgcg agcacaacat gttgaaatgg tagaacattt
     1081 ttataaatta ggtgccgacc aagtaattcc tcaaagtttt gaattggcca tagatttgtt
     1141 tcatagagta ttaagtaaac gacttattcc tttgaaagaa accaatagaa taatttcaag
     1201 tattcgtagt gattatttag gtgtgtatag cgacaaagat ttacaacatc aggcatcaat
     1261 actcgaagag ttatcgcata ttgaaataag tgccataaaa atagaagaac attcggaatt
     1321 agtaggaaaa acattgcaag attctgaatt gcgtaagcga acaggtgtaa ctttacttgc
     1381 aattaaacga ggtaaaacag taatagaaca ccctaattta caatttatat ttgcgtgtaa
     1441 tgatattgca tttgttctgg gaagtcccga acaagtgaat gctgcatctg agatttttag
     1501 tgtgtaaaat ttatttcttt ttgcctcgag ctgcttgcag agtattcata agtaaagagc
     1561 aaatagtcat aggtcctaca ccaccgggta ctggtgttat ataactacaa agaggtgcta
     1621 cttcatcaaa ttttacatcg ccttttaatt tccaacccga ttttgtttcg ttagaagcta
     1681 cgcgagttgt tcccacgtct attacaacgg caccgggttt taccatatcg gctgtaacaa
     1741 attcaggtga accaagggct gctattaata tatcggcacg tttacattct tctgctaaat
     1801 gttttgtgcg actatgacaa actgtaacgg tacagtcgcc gggataacct ttttgtgcca
     1861 acataacact gagcggtttt cctactatat tacttcttcc tataattaca cagtgttttc
     1921 ctgctgtttc tatatgataa tattttaata attctaaaat acctgcaggt gtagccgatt
     1981 taaaagaagg taacccaatc atcatttttc cgacatttat tgggtgaaat ccgtctacat
     2041 ctttgtcggg tgaaatagct tctattactt tttgttcatc aatatgtttg ggaagtggca
     2101 gttgtacaat aaatccgtca atatctttat ttgaattgaa ttcggcaatt gtgtcgagta
     2161 atgcatcttc agaaatatct tcgtcaaatg ttttgagtgt agaggcaaat cctacttgtt
     2221 cacaagcttt tactttgtgg tttacatagg ttatactgcc accgtcggtt ccaactaaaa
     2281 tagcagccaa atgaggaatc tttccacctt gcgattgaat tattgtaact tcgtcagcaa
     2341 tttgttgctt aattgcatca cttacttttt tgccatctat taattccata gtattacatt
     2401 tttaaaccgg gcattaaatt ttgcatcatc ttattacctt ttgccccttg catcattttc
     2461 atcatttttc gagtttcatc aaactgtttt attagtttgt ttacttcttg aatacttgta
     2521 ccgcttccgt cggctatacg tttccgtcgg ctaccgttaa gaattattgg gttagcacgt
     2581 tcttcaggag tcatagaatg tataattgct tctattcctt taaatgcatt atcatctata
     2641 tcaatatttt tgagcatttt acccattccc ggaatcatgc ttgccaaatc tttgaggtta
     2701 cccatttttt tgatttgttg aatttggctc aaaaagtctt caaaattgaa ttggtttttt
     2761 gatattttct tttgcagttt tcgtgcttct tctgcatcat attgttcttg agctctttcc
     2821 actaatgata ctatatcgcc catacccaaa attcggtcgg ccatacgttc ggggtgaaat
     2881 acatctattg cttctaattt ttcgcctgta cctacaaatt ttataggttt gtttacaaca
     2941 gagcgtatag aaagcgccgc accaccacgt gtatcaccat ctaattttgt aagcacaaca
     3001 ccactaaagt caagtctgtc attaaattct tttgcagtat ttactgcatc ttgacctgtc
     3061 atagagtcaa caacaaacaa tatttcgtgt gggttaatag catttttaat gtttgtaatt
     3121 tcggtcatca tggtttcatc tattgctaaa cgaccggcag tatctactat aacacaatca
     3181 aaattatatt cgcgagcatg ttttatagct gattttgcaa ttgaaacagg gtctttgctt
     3241 gtaatgtcag caaatacagg aacttgaatt tgttctccta atacttgtaa ttgattaatt
     3301 gcagcgggtc tataaacgtc acatgctaca agaagtggtt ttttgccttg tttattttta
     3361 agcatatgag ccaatttacc cgacatggta gttttacccg aaccttgtaa tccggccata
     3421 agaataattg caggtgaacc tttggtgtta agttctgttt tggttccacc cataagtaaa
     3481 gcaagctcat catgaacaat ttttgtcatc atttcgcccg gttttacagc cgttaaaaca
     3541 ttttgaccaa gtgcttttac ttttacgtct tcggtaaatt gttttgcaat tttataattt
     3601 acgtcggcat caagcaaagc ccgacgtact tctttgaggg tttcggctac gtttatttcg
     3661 gttatttttc catacccttt gagtaatctg aatgactgtt ctaagcgttc cgataaattt
     3721 tcgaacatat actattgttt tat
//
LOCUS       DAOTOW010000134         3742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19572_length_3742_cov_3.150800, whole genome shotgun sequence.
ACCESSION   DAOTOW010000134 DAOTOW010000000
VERSION     DAOTOW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19572_length_3742_cov_3.150800"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="P5243_03325"
     CDS             <1..1960
                     /locus_tag="P5243_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS18512.1"
                     /translation="EPLNEASVSTEASFSGSLMPDKVTSTRYVVAKGTAATLDFAAVM
                     AMAYRIYKPYYSAFADSCLAAAEYAWQWAKSNPNKAYTNPPAIGSYPAITTGGYGDNY
                     FNDERIWAATELLISTNKQDDYAASINLQETFNIPMWRNVAMLSLYSLYTHRQTIGYC
                     IDTAKVKDILIIKARSLQTLQSSSNPYRVPVTDFVWGSNGVVANQIVLLLFGFNITHD
                     VSYLNAATASFDYILGRNATEYSFITGYGTKATRNIHHRPSEADGIAGSIPGFLAGGP
                     NGGSKTDCTGEYSQFPAKAYRDEICSYTTNEIAINWQSPLTFAAHGIISAYNTWLHEL
                     PAEYAYPSTDSFSVTRTQETASFTVFANTPFTIETTDTWYTVTPIQYTGSSTVTIAIT
                     QFNEGDSIRTGTMYIIVNNSVFDSITISQIGKLKNFRIQAEDFMSMSGVQTETTSDID
                     GGLNVGWIDNNDYMIYRIDITTKGSYIINYRYASLSAKSEFFMVYKDSVYSLAQTNPT
                     GGWQVWRTYSDTAYFEEGIYDIQLLSQYGGFNLNYIDFSWICEDNIAAINIPTTPPLP
                     PDPPISITHIQNSEIYISTPVKSNILTIHGIDSNQSFSFCIYNTLGQIVQSGNSNSSN
                     ISFNNNAGVYICTIRINNKMWNCYFIKE"
     gene            1966..3742
                     /locus_tag="P5243_03330"
                     /pseudo
     CDS             1966..3742
                     /locus_tag="P5243_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
BASE COUNT         1318 a          665 c          639 g         1120 t
ORIGIN      
        1 tgaaccgcta aatgaagctt cggtatctac cgaagcttca tttagcggtt cacttatgcc
       61 tgataaagta acatcaacac ggtatgtagt agcaaaagga actgccgcaa cacttgattt
      121 tgccgcagtt atggcaatgg catacagaat ttataaaccg tattattctg catttgcaga
      181 ttcttgttta gctgccgcag aatatgcgtg gcaatgggca aaatcaaatc ctaacaaagc
      241 atatacaaat ccaccggcaa taggtagtta tcctgctatt actacaggcg gatacggtga
      301 caattacttc aatgatgaac gaatatgggc tgccacagaa ttactgattt caaccaacaa
      361 acaagatgat tatgctgcaa gcattaattt acaagaaaca ttcaatattc caatgtggcg
      421 caatgtagct atgttatcgc tgtattcatt gtatacacat aggcaaacta tcgggtattg
      481 tatagatact gccaaagtaa aagatatttt aattataaaa gctcgttcat tgcaaacact
      541 tcaatcaagt agcaacccat atagagtacc tgtaactgat tttgtatggg gaagtaatgg
      601 cgtagttgca aatcaaattg ttttattatt atttggattc aacattacac atgacgtgtc
      661 ataccttaat gcagctacag cgtcatttga ttatatacta gggcgtaatg ctaccgaata
      721 ctcttttata acaggatatg gaacaaaagc cactcgcaat atacatcatc gcccttcaga
      781 agctgacgga atagcaggtt ctattccggg atttttagcc ggaggtccca atggcggtag
      841 taaaactgac tgtacaggtg aatactctca attccctgca aaagcctatc gtgatgaaat
      901 atgcagttat acaaccaatg aaattgcaat aaattggcaa tctccactta cgtttgctgc
      961 tcatggaatt ataagtgcat ataatacatg gttgcacgaa ttacctgcag aatatgcata
     1021 cccttcaacg gattcctttt cggttactcg cacacaagaa acagcttcgt ttacagtatt
     1081 tgctaatacg ccattcacta tagaaaccac cgatacttgg tacaccgtta ctccaataca
     1141 atatacagga agcagtactg ttactattgc aattactcaa ttcaatgaag gtgacagtat
     1201 acgaaccggc actatgtata ttatagtaaa taattctgtt tttgattcaa ttacaatatc
     1261 acaaattggc aaactcaaaa actttagaat tcaagcagaa gattttatga gtatgagtgg
     1321 cgtacaaaca gaaaccacaa gtgatattga tggagggctc aatgtaggtt ggatagataa
     1381 taatgattat atgatatata gaatagatat tacaacaaaa ggttcatata ttattaacta
     1441 tcgctatgct tcattatcag caaaaagtga attttttatg gtttataaag attctgtata
     1501 ttcacttgct caaacaaatc ccacaggagg ttggcaagta tggcgaacat attcagatac
     1561 agcatacttt gaagaaggaa tatatgatat tcaattacta tcacaatatg gtggatttaa
     1621 tctcaattat atagatttct catggatttg tgaagataat attgcagcca taaatatacc
     1681 aacaacacct cctctgcctc ccgatcctcc aatttcaatt acacacatac aaaattctga
     1741 gatttatatt tcaacccctg taaaatcaaa catacttacc atacatggaa tagattcaaa
     1801 tcaatctttt tcattttgta tttataatac attaggacaa atagtacaat caggaaattc
     1861 aaattcaagt aacatttctt ttaacaataa tgccggtgta tatatatgta caattagaat
     1921 aaacaataaa atgtggaact gttattttat taaagaataa gatacatgaa gctactatat
     1981 tatgtgtaca ttatattatt attgttagtt aataatatat gtaatgctca aagtaaagag
     2041 cctaccaaat atatacgaat gaatcaaata ggttttttac cgtatgctcc taaaattgct
     2101 gcaattgttg atactaaagc caaagaattt gagatatata acaacaaaaa acaattggta
     2161 tatacaggta tactttctcc ggcaaaagaa tggtcgcaat caggagaaaa agttcaaatt
     2221 gcagatttta ctacaattac tacaccggga acatacacat tacaaataaa agacatagct
     2281 gtatcttata catttgctat tcaatcaaac gtattttaca acgttaacaa tgcaataatt
     2341 aaagcattct atttcaatag agcatcgatt gaaattgaac ctcaatatgc gggtatattt
     2401 gctcgcaaag caggacatta cgatacacaa gttattgtat taccttctgc agctggacct
     2461 aagcgtaaag caggcgatat aatttcatgt cccaaaggct ggtacgatgc cggagattat
     2521 aataaataca ttgtaaattc aggtattaca gtaggcacat tactcttggc ttacgaacgc
     2581 tatgcatcat attttgatac acttacatgg aatattcccg aaagtaacaa ttccattccc
     2641 gatatacttg atgaagtaaa atggaattta gactggatgc tttcaatgca agattctgat
     2701 gatggtggag tatacaataa aactaccgat gccaattttt gtggaatgat aatgccgcat
     2761 gaagcttctg tttctaaacg atatgttgta gcaaaaggaa catcggcaag tttaaatttt
     2821 gcagcttcta tggcaatggc atataggata tataaaccct ttgattctgt atatgccaat
     2881 acttgtttga aagcagctat taaagcgtgg gattgggcag aactaaatcc gaatatcccc
     2941 tataataacc ctcaacaaga tggagaattt cctaaaataa caaccggagg atatggcgac
     3001 aaatcatttg ccgatgaaaa aacatgggct gcagcagaat tacttattgc cacaaacaat
     3061 acacaaaaat atgcatcata tattgacgta caaactaact ttggaattcc tgtttggttt
     3121 aatgtaggag gaatggcttt atattcttta tataatcaca gacaacaagt gtgcaaaggt
     3181 gtagatacaa caactgtaat agaccgaata cttgaacaag ctcacaaatt acaaaactac
     3241 caagaacatg aaaacgcata caaagtaccg gttgttgatt tttcgtgggg tagtaatggc
     3301 gtattggcaa atcaaggcat tctttttttg tatgcatatg caataacaaa aaactataat
     3361 tacttcacct cagctcttgc ttaatttgat tatatactag gacgtaatgc cacagaatat
     3421 agttttgtga ccggctatgg atacaagtat acacaaaata tacatcatag accctcagaa
     3481 gcagatggta ttgccgggtc tattcccggt tttgttgcag gaggaccaaa tggaggtcaa
     3541 aaacgggatt gttttggaaa ttactcccct tttgctgcaa aagcatatta tgatgcgaca
     3601 tgcagttata ccacaaacga agttgctata aattggcaag cgccattggt atttttagca
     3661 catggtatag ttgccgaata ttacaatatt gaaaaacaat tactaacaag ctcagcatat
     3721 tctccatatt ctgaaatcat at
//
LOCUS       DAOTOW010000135         3741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19579_length_3741_cov_2.653554, whole genome shotgun sequence.
ACCESSION   DAOTOW010000135 DAOTOW010000000
VERSION     DAOTOW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19579_length_3741_cov_2.653554"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..1210
                     /locus_tag="P5243_03335"
     CDS             545..1210
                     /locus_tag="P5243_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18513.1"
                     /translation="MEKKITCIETLPFSITNIDLYQTQLFNRLDSKNNLGGLLSFRKF
                     VLEAFGDAVTFERDRNTENSPYKRMHRYLKKQFESIQLEKNDKLVIVAESMGVQLLYT
                     YICDADLQAGIFEKEKSNDTNNLRNLSYLATCGCNLPLFFSGFPESEIIAFEKRNSQF
                     IWENFYDKNDVLGWPLNQLSSSFNSLVNDYEINTGMYIGSHVKYWGDNSFTKHFFNTI
                     LSL"
     gene            1223..1768
                     /locus_tag="P5243_03340"
     CDS             1223..1768
                     /locus_tag="P5243_03340"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRS18514.1"
                     /translation="MENIDLYTYVIIPLLIFFARICDQSIGTLRIIFISKGYKYLAPL
                     IGFFEVLIWVLAITKVMANLDNWICYVAYAGGFATGNYVGMLLEERLALGYEMIRVIT
                     KVDATNLIQAIRSMGYTATVVEAQGAEGKVGVIYIIINRKCIEKIVHVIQEHNPNALY
                     TIEDIRFVNKDIRPWIPQEER"
     gene            complement(1769..2998)
                     /gene="serB"
                     /locus_tag="P5243_03345"
     CDS             complement(1769..2998)
                     /gene="serB"
                     /locus_tag="P5243_03345"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRS18515.1"
                     /translation="MNNQSQEVILLSISGKDRPGVTASLTAILAQYGADILDIGQAVI
                     HDTLSLGILFRLPESNVSGPVLKELLFKAYEFDIQARFTPINNTTYEDWVQQQGKNRY
                     IVTLFGRHIEARHIAEITRILYRQTLNIDNITRLSGRVSFKKESIAKNRACVELSVRG
                     TPVNEQEMKRDFLEISKQIGLDIAFQEDNAFRRNRRLICFDMDSTLIQTEVIVELAKY
                     AGVEKQVSEITESAMRGEIDFVESFKQRISLLKDIDESVLDEIARNLPITEGADRLIR
                     TLKRFGYKVAILSGGFSFFGRYLQTKLGVDYVFANELEIKNGKLTGNYIGDIVDGAMK
                     AELLKKLAFKEDIHLEQVIAVGDGANDLPMIQIAGLGIAFHAKPTVQEKANNKISTIG
                     LDGILYMLGFRDREVDD"
     gene            complement(3009..>3741)
                     /locus_tag="P5243_03350"
     CDS             complement(3009..>3741)
                     /locus_tag="P5243_03350"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRS18516.1"
                     /translation="EAKIRDKNDTITGFSKVKIIESLSASGSYNFAADSLGMSLIQIG
                     GYTTLAKRIRINYSATFDPYALGLKQQNSSKQVVRTNSYMLDKYGTLWRKTDESWRTG
                     ISYSFGPIKQENTPKHSGEYSYWNVPWNLNVGYNISVPRKYYYNYNNELDSVSQKIQQ
                     DLSLNGTLSLSKKWNINFATGWDFKEKALSYTSIDIYRDLHCWNMKFNWIPLGERQRW
                     EFILRVNADMLKDLKYEMKSQTNYF"
BASE COUNT         1277 a          556 c          529 g         1379 t
ORIGIN      
        1 caccaattgt agtaaaactt ttaagtttaa cacttattgt tttatttttc acttcgtttg
       61 caaacagttg aaaaatattt gtttagagat gttttttctt tcgaaaaaat gaaaaaatca
      121 atgaatgatt tacaacatga atataaaaat ctgaaatatt ttgaaagtca aaaactaaaa
      181 gtaaataaaa aagaaatgca tagaattgaa tatttattaa actcatataa tttaataaat
      241 atcaattaat tagcataaat aataatacac aattacgtat ccacatttgt aatttacaca
      301 tactcccgta tgtgtttggt ctataataga ttaatattga attatatatt aattatgtaa
      361 caaaagttaa tatttgtttt taattatgta ttttacaata aattaattaa aataatgtaa
      421 ttttacatat attaaaaaaa aaatcatgat ttatattgta acaattccag gtattggttc
      481 aaaaaaaaac ggttacagta aaaaatttgt caccgatatt tatcattttt ccaaacatac
      541 acacttggaa aaaaaaataa catgtattga aacattacca ttttcaatta caaatataga
      601 tttatatcaa acacaattat ttaatagact tgattctaaa aataatttgg gcggattgct
      661 tagttttaga aaatttgtgt tagaagcttt tggtgatgct gttacctttg aacgtgatag
      721 aaatactgaa aatagtccat ataaacgaat gcatagatat ttgaaaaaac aatttgaatc
      781 tatacaatta gaaaagaatg ataaacttgt aattgttgca gaatctatgg gtgttcagct
      841 attatataca tatatttgtg atgctgattt acaggcagga atatttgaaa aagaaaaatc
      901 aaatgataca aataatttac ggaatctctc atatttagca acatgtggtt gtaatttacc
      961 tcttttcttt tccggctttc cagaatctga aattattgcg ttcgaaaaaa gaaattctca
     1021 gtttatttgg gaaaattttt atgataaaaa tgatgtttta ggttggcctc ttaatcaatt
     1081 gagctcttct tttaattctt tagtcaatga ttatgaaatt aatactggca tgtatatagg
     1141 tagtcatgta aaatattggg gtgataattc atttacaaag cattttttta atacaatatt
     1201 atcattataa tacttctctc ctatggaaaa tattgatttg tatacctatg tcataattcc
     1261 gcttctaata ttttttgcac gtatttgtga tcaatctatt ggtacacttc gtattatttt
     1321 tatctcaaag ggatataaat atttagctcc acttatcgga ttttttgagg tgttaatttg
     1381 ggtgcttgct attactaagg ttatggcaaa tttagataat tggatttgct atgttgcata
     1441 tgctggcgga tttgctaccg gtaattatgt aggaatgtta ttggaagaac gattagcgct
     1501 tggttatgag atgattagag ttattacgaa ggttgatgct acgaatttaa tacaagctat
     1561 tcgcagtatg ggatatacag ctacagttgt tgaagcgcaa ggagctgaag gtaaagttgg
     1621 tgtaatatat ataattataa atagaaagtg tatagaaaaa attgtacacg taattcaaga
     1681 acataatcca aatgctttgt atactattga agatatacga tttgttaata aagatatacg
     1741 tccttggatt ccacaagaag aacggtaact aatcgtcaac ttcacgatct ctgaaaccta
     1801 acatatataa tatgccgtct aaacctattg tactgatttt attgttcgct ttttcttgta
     1861 ctgtaggttt tgcatgaaat gcaattccta aaccggcaat ttgtatcata ggtaaatcat
     1921 tagcaccatc gccaacggca attacttgtt ctaaatgtat atcttcttta aatgccagtt
     1981 ttttgagtaa ttcggctttc atagcaccat ctactatatc acctatataa ttaccggtaa
     2041 gttttccatt tttaatttct agttcatttg caaatacata atcaacgcct aattttgttt
     2101 gtaagtatct gccaaaaaaa ctaaaaccgc ccgagagaat tgccacttta tatccaaacc
     2161 gtttaagagt acgtatcaat ctgtcggcac cttcggttat aggtaaatta cgtgctattt
     2221 catcaagtac agattcatca atatctttta ataaagaaat acgctgttta aaactttcta
     2281 caaaatcaat ttcacctcgc attgcagatt ctgtaatttc agatacttgt ttttctacac
     2341 cggcatattt agctaattca acaataactt ctgtttgtat aagtgtagaa tccatatcaa
     2401 aacatattaa acggcgattt cgtctaaatg cattatcttc ttgaaatgct atatctaaac
     2461 ctatttgttt tgaaatttca agaaaatccc gtttcatttc ttgttcattt accggagttc
     2521 ctcttactga taattctaca caagctctat tttttgcaat actttctttt ttaaatgata
     2581 ctctaccact taaacgagta atattatcaa tatttaaagt ctgacgatat aaaatacgag
     2641 taatttcagc aatatgacga gcttctatat gtctgccaaa taacgtaact atatatctat
     2701 tcttaccttg ttgttgaacc caatcttcat atgtagtatt atttattggt gtaaatcgag
     2761 cctgaatatc aaattcatat gctttgaata ataattcttt taatacagga ccgcttacgt
     2821 ttgattccgg caatctaaat aaaatgccta aagataacgt atcgtgtatt accgcttgac
     2881 caatatcaag aatatcagct ccatattgtg ctaatatagc agttaatgat gcagtaacgc
     2941 caggtctgtc tttaccggat atacttaaca atataacttc ttgtgattga ttattcataa
     3001 aaaaaatatt aaaagtaatt tgtttggctt ttcatttcat atttcaaatc ttttaacata
     3061 tcggcattta cacgtagaat aaactcccat cgttgacgtt cgccaagagg aatccaatta
     3121 aatttcatat tccaacaatg caaatcgcga tatatgtcta ttgatgtata cgataaagct
     3181 ttttctttaa aatcccaacc ggtagcaaag ttaatattcc atttttttga aagactcaat
     3241 gttccgttta atgataagtc ttgttggatt ttttgtgaaa ccgaatctaa ttcgttattg
     3301 taattataat aatattttcg aggaacactt atattgtatc caacatttaa gttccaaggt
     3361 acattccaat aactatattc tccggaatgt tttggggtat tttcttgttt aattggaccg
     3421 aatgaataac taatacctgt acgccatgat tcatctgttt ttcgccataa cgtaccatat
     3481 ttatcaagca tgtatgaatt agttcttaca acttgtttag aagaattttg ttgtttcaat
     3541 cctaatgcat atgggtcaaa agttgctgaa taatttattc tgattctttt tgcaagtgta
     3601 gtatatccac ctatttgaat cagactcata ccaagagaat ctgcggcaaa attatatgaa
     3661 ccgcttgccg ataaactttc aataatttta actttagaaa aaccggtaat ggtatcattt
     3721 ttatctcgta ttttagcttc t
//
LOCUS       DAOTOW010000136         3727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19670_length_3727_cov_3.460512, whole genome shotgun sequence.
ACCESSION   DAOTOW010000136 DAOTOW010000000
VERSION     DAOTOW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19670_length_3727_cov_3.460512"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="P5243_03355"
     CDS             <1..333
                     /locus_tag="P5243_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18517.1"
                     /translation="VPDVNLHSIAYDAGVVLELAPRFDVLAGAKLLTVEGTEYASVRN
                     EFNEITDYSVFSLNNKQTLYAGGIRYRFNDNIALTLQGSTYNVTHVQKSEYDFSIQQV
                     YMIFSMKF"
     gene            350..1345
                     /locus_tag="P5243_03360"
     CDS             350..1345
                     /locus_tag="P5243_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HRS18518.1"
                     /translation="MKKIVFYILAATITTNLFLGCEKEDPIGPNLVDLYGPFRILDSL
                     TYTNSGVINFKTDSVLQFSGSWTNMAEWKIEIKGRTSGAVKTITGKSKNLDSIAGQWN
                     GSADGIFFRKEVCDVVFSFKDYPDTMKTTVTIAEPRDYSKVGYIIKHFEGSGAEEFWG
                     GNGKQAKRINKSDAPEGTNYFYMEGTEGGSSYWIGSFPPVSAQSIVAKPYFPLNDADT
                     ATTYINFFVRGYGTANTFINFQILEDDNGDGVYTEASEDSFSYRYQVPTTATTWQKVS
                     IPLSKLQTEANAAGNSKMEISKITSMFVMLFSNGIATSKLACDIDFVIITKGKPL"
     gene            1377..2594
                     /locus_tag="P5243_03365"
     CDS             1377..2594
                     /locus_tag="P5243_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18519.1"
                     /translation="MKKTIHVASIILVMFSLSSCVLFLPMALNTADDNDPYFVINKEV
                     NSDYIEKFTATDIFTDDIRNTWGTEKDACREFIVVDSVKKAGAASLLITWDKSKKGCD
                     WIGVGWAWNNWQTIDMSTILDTWAIRFWVRTKKGTLPNVPLILSFIDDANKTTEYVSV
                     SEKFYKGPGIDETWKEVIIPLSYFRFISKGVNVTSVSQLITTFEGSASIYIDELQLIN
                     IAKTDASGTKKETGLSATSVYSDKVLNAWGIEKDACREFVEVSDVKKNGSSSLYLSWN
                     TAKSGCSVISAGFAWNNWKVVNMTEVMDSWALHFWIRTKEGSMQTVPMFISLQDDGSI
                     VSKSVSISNAYYKGAAIDETWREVTIPLSHFKAKEEGLNLYEITQIIMTFEGASAVYI
                     DDMSFIQLAKSTK"
     gene            2603..>3727
                     /locus_tag="P5243_03370"
     CDS             2603..>3727
                     /locus_tag="P5243_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HRS18520.1"
                     /translation="MNKIFRLHSIVVLIIVLISSVVFSQNTPVYPSYNTSPKAPDTTG
                     MRSNAIQLAAKMHLGWNIGNTMEATGGETAWGNPMVTKQLIDAVKKAGFNAIRIPCAW
                     NQYLSNAAKAEIDKNWLKRVKEVVDYCIDNDMYVLLNIHWDGGWLENNCTPQYKDQNN
                     AKQKAFWEQIATYFRDYDERLLFAGANEPNVENAEQMAVLLSYYQTFVDAVRSTGGRN
                     AYRILVVPGPSTDFEKTYKLMHTFPTDVVPNRLMLEVHFYTPYTFCGLTEDANWGRMT
                     YYWSKKYYSKTDTLRNSYWGEEDEIVKLFGMIREKFVDKGIPVLVGEYGVVRRSHLTG
                     ESLALHLNSRAYWLRYVTQLCVENQMVPFYWDAGGTGANSW"
BASE COUNT         1270 a          550 c          740 g         1167 t
ORIGIN      
        1 gtgcccgatg taaatttaca ttcaattgca tatgatgccg gggtggtgct agaattagca
       61 ccgcgatttg atgtgttggc cggggctaaa ttgctcactg tagaaggaac tgaatatgca
      121 agtgttcgta atgaatttaa tgaaattacc gattattcgg ttttttcgct caacaataag
      181 caaacattat atgccggagg gattcgttat agatttaatg ataatatagc tcttacattg
      241 caaggtagta catataatgt tacacatgta cagaagtcag aatatgattt ttcaattcaa
      301 caagtgtata tgatattttc aatgaaattc taataaaaaa gtcaaggtta tgaaaaaaat
      361 agtattctat atactcgctg caacaattac aacgaatttg tttttaggtt gcgaaaaaga
      421 agacccaatt ggaccaaatt tggtagattt atatggacca tttcgaatat tggactcctt
      481 aacatataca aattcaggcg tcattaattt taagaccgat agtgtacttc aattttcagg
      541 ctcatggact aatatggcag aatggaaaat tgaaatcaaa ggaagaactt cgggagctgt
      601 gaaaacaatt accggaaaat caaaaaatct tgattcaatt gccggacaat ggaatggtag
      661 tgccgatggc atttttttta gaaaagaagt atgcgatgtg gtgttttcgt tcaaagatta
      721 tcctgatact atgaaaacaa cggttacaat tgctgaaccg cgagattatt ctaaagttgg
      781 ctatattatt aaacatttcg aaggttcggg agccgaagaa ttttgggggg gcaatggaaa
      841 gcaagcaaaa cgtatcaata aatctgatgc tcctgaaggt actaattatt tctatatgga
      901 aggtactgaa ggcggaagtt catattggat aggaagtttt cctcctgttt cggctcaatc
      961 aatagttgct aaaccatatt ttcctctcaa cgatgctgat acagcaacta catatattaa
     1021 cttttttgtt cgtggatatg gtactgccaa tacatttata aactttcaaa tattagaaga
     1081 tgataatgga gatggtgtat ataccgaagc aagtgaggat tcgttttcat atcgatatca
     1141 agtgcctacc acagctacta catggcaaaa agtttcaata cctttaagca aactacaaac
     1201 cgaagcaaat gctgcaggaa acagcaaaat ggagataagc aaaataactt ctatgtttgt
     1261 aatgcttttc tcaaatggaa tagctacttc taaattggca tgcgacattg attttgtaat
     1321 aataactaaa ggtaaaccac tttaaaaaat agacaatgta tttaaactcg attactatga
     1381 aaaaaacaat acatgtagca agtattatac ttgtaatgtt cagtttaagc agttgtgttc
     1441 tctttttgcc tatggcttta aacactgccg acgataatga cccatatttt gtaattaata
     1501 aagaagtaaa ttctgattat attgaaaaat ttacggcaac cgatattttt accgatgata
     1561 ttcgaaatac ttggggaacc gaaaaagatg cttgtagaga atttattgtt gtagattctg
     1621 taaaaaaagc aggtgcagca tcgcttctta ttacatggga taaatctaaa aaaggttgtg
     1681 actggattgg tgtaggatgg gcttggaata attggcaaac tattgatatg tcaacaattt
     1741 tagatacatg ggctattcgc ttttgggttc gtaccaaaaa aggaacgtta ccaaatgtgc
     1801 cgcttattct tagttttata gacgatgcca acaaaacaac tgaatatgtg tcggtatcag
     1861 aaaaatttta taaggggccg ggaatagatg aaacatggaa agaagtgatt attccgttat
     1921 catattttcg ttttatttcc aaaggagtta atgtaacatc agtttcgcaa ttaattacaa
     1981 cttttgaagg ttcagcatct atatatattg atgaattaca acttataaac atagcaaaaa
     2041 cagatgcttc gggaactaaa aaagaaacag gattatctgc tacttcagta tattcagaca
     2101 aagtactaaa tgcatggggt atagaaaaag atgcttgtag agaatttgtt gaagttagcg
     2161 atgtgaaaaa gaatggttct tcatcgttgt atttatcgtg gaataccgct aaaagtggat
     2221 gtagtgttat ttctgcaggt tttgcatgga ataattggaa ggttgtaaat atgaccgagg
     2281 ttatggattc gtgggcgtta cacttctgga ttcgcactaa agaaggtagt atgcaaactg
     2341 tacctatgtt tattagttta caagatgatg gttctatagt gtcaaaaagt gtaagtatat
     2401 caaatgccta ttataaagga gctgcaatag atgaaacttg gagagaagtt actattcctc
     2461 tctcacattt taaagccaaa gaagaaggat tgaatctgta tgagattact caaataatta
     2521 tgactttcga aggagcatcg gctgtatata ttgatgatat gtcgtttatt caacttgcta
     2581 aatctactaa ataataccct ttatgaataa aatttttcga ttacatagta ttgtagtact
     2641 tattatagta ttaatttctt cagtagtttt ttctcaaaat actcctgtgt atccaagtta
     2701 caatacttca cccaaagccc cggataccac aggtatgaga agtaatgcca tacaattggc
     2761 tgctaaaatg catttaggtt ggaatatagg caataccatg gaagctaccg gaggtgaaac
     2821 tgcttgggga aatcccatgg ttaccaaaca attgatagat gctgttaaaa aagccggatt
     2881 taatgcaatt cgaattccgt gtgcttggaa tcaatatctg tcaaatgcag caaaagctga
     2941 aattgataaa aattggctta aacgagtaaa agaagtagta gattattgta ttgacaatga
     3001 tatgtatgta ttgttaaata ttcattggga tggagggtgg ttagaaaata attgtacacc
     3061 gcaatacaaa gatcaaaata atgcaaaaca aaaagcattt tgggaacaaa tagctactta
     3121 cttcagagat tatgatgaac gattactctt tgccggagca aatgaaccaa atgttgagaa
     3181 tgcagaacaa atggctgtat tactttcata ttatcaaaca tttgttgatg ccgttcgttc
     3241 taccggaggc cgaaatgcat accgtatatt agtggtgccg gggccttcta cagattttga
     3301 aaaaacatat aaacttatgc atacattccc taccgatgtt gtaccaaata gattgatgct
     3361 tgaagtccat ttttatactc cgtatacttt ttgcggctta accgaggatg ctaattgggg
     3421 acgtatgact tattattgga gtaaaaaata ttattcaaaa accgatacgc ttcgaaattc
     3481 atattggggc gaagaagatg aaattgtaaa attatttgga atgatacgcg aaaagttcgt
     3541 agataaaggt attcctgttt tagttggaga atatggtgtt gttcgacgtt ctcatttaac
     3601 aggagaaagc cttgctcttc atttaaattc aagagcttat tggttacgat atgttaccca
     3661 actttgtgtt gaaaaccaaa tggttccatt ctattgggat gccggaggaa caggtgccaa
     3721 ttcatgg
//
LOCUS       DAOTOW010000137         3722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19706_length_3722_cov_3.604854, whole genome shotgun sequence.
ACCESSION   DAOTOW010000137 DAOTOW010000000
VERSION     DAOTOW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19706_length_3722_cov_3.604854"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1570)
                     /gene="gltA"
                     /locus_tag="P5243_03375"
     CDS             complement(92..1570)
                     /gene="gltA"
                     /locus_tag="P5243_03375"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRS18521.1"
                     /translation="MTYQDYLKEQRELAWRAELRKQIPAKERTSKERVQMPEQDAHNR
                     SINHEEVNLGLTAEMAVNEAQRCLDCVTPTCVEGCPVGINIPGFIKCIETKDFLGAAK
                     VLKQTNALPAVCGRVCPQEIQCESKCFYTIKMNKPAVAIGNLERFAADYERESGTISI
                     PEVAAPNGFKVAVIGSGPAGLAAAGDLIKAGCKVTVFEALHEIGGVLKYGIPEFRLPN
                     YMVDIEINNLKQMGVEFITNFVVGSTASIQDLRDEGYQAFFVSSGAGLPNFMKIPGEN
                     YSGILSSNEYLTRVNLMGAAKKEYDTPVFLGKNVAVIGGGNTAMDSVRTAKRLGAERA
                     MIIYRRSMDEMPARKEEIHHAIEEGVEFLCLNNPIEYFADTKGYVNKMRVQKMELGEP
                     DASGRRKPVPMIGSEYDIDVDLVIVSVGVSPNPLIPSSLPQLEMSKWGTIVVNKDTMQ
                     SNIPDIFAGGDIVRGGATVILAMGDGRKAAQGILTYLHEQKK"
     gene            complement(1584..2408)
                     /locus_tag="P5243_03380"
     CDS             complement(1584..2408)
                     /locus_tag="P5243_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRS18522.1"
                     /translation="MNKIVTKEILSDLVVKLEIEAPRIAKKRKAGHFVIVKIGEKGER
                     IPLTISQADTQKGTITLILQKVGVTSHKLAMLNEGDYITDVVGPLGKATHIENFGTVL
                     CAGGGVGIAPLLPIIQDMKAAGNKVISVLAARTKELLILEQEVRACSDEVIIMTDDGS
                     YGQKGLVTQGMEDVIQREKVHMCVTIGPAIMMKFTSMLTKKYNIPTVASLNTIMVDGT
                     GMCGACRISVGGQTKFVCVDGPEFDAHQIDFEEMLMRLGAYKNQEAEAYQSYLQQV"
     gene            complement(2608..>3722)
                     /gene="gdhA"
                     /locus_tag="P5243_03385"
     CDS             complement(2608..>3722)
                     /gene="gdhA"
                     /locus_tag="P5243_03385"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRS18523.1"
                     /translation="GYRIQMNSAIGPYKGGIRFHPTVSLGVLKFLAFEQVFKNSLTTL
                     PMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGADTDVPAGDIGVGGREVGYMY
                     GQYKRLRNEFTGVLTGKGIGWGGSLIRPEATGYGQVYFAEEMLKTRGDSMKGKVCVVS
                     GSGNVAQYAIEKATQLGAKCVTASDSNGFIYDPAGIDAEKLAFIMELKNNNRGRIKEY
                     ADKYKCEYHEGKTPWGIKCDVALPSATQNEINGDDARTLIKNGCICVSEGANMPSTPE
                     AIEVYIQAKILYGLGKAANAGGVATSGLEMTQNSMRLSWTREEVDAKLQSIMKNIHAT
                     CVKYGTEKDGYINYVKGANIGGFIKVADAMIAQGVI"
BASE COUNT         1043 a          779 c          567 g         1333 t
ORIGIN      
        1 gttttatctt tgcaattaag ttatcataaa caaccttgtg aaacttgaca gttataccta
       61 aatatttaac tgaagtatca atcatagttt cttatttttt ttgttcgtgt aaataggtta
      121 aaattccttg agcagctttt ctaccatcac ccattgctaa aataacagta gcgccacctc
      181 ttactatatc accaccggca aatatatcag gtatattgct ttgcatagtg tctttgttta
      241 caactatagt accccatttg ctcatttcta attgaggcaa actgcttgga ataagcggat
      301 taggcgatac tccaacactt acaataacaa ggtctacatc aatatcgtat tcagaaccaa
      361 tcattggaac cggttttcgt cgtcctgagg catcaggttc gcctaattcc attttttgaa
      421 cacgcatctt atttacatat cctttagtat ctgcaaagta ttctatagga ttatttaagc
      481 acaaaaattc aactccttct tctatggcat ggtgaatttc ttctttacga gcaggcattt
      541 catccatgct tctgcgatat ataatcatag ctcgttctgc accaagtcgt tttgccgttc
      601 tcacagaatc catagcagta ttgccaccac ctattacagc aacattttta cctaaaaata
      661 caggagtatc atattctttt tttgcagctc ccattaagtt tactcgtgtt agatattcgt
      721 ttgacgaaag gattcctgaa taattttcgc ccggaatttt cataaaatta ggtaatcctg
      781 caccactact tacaaaaaat gcttgataac cttcatcgcg taaatcttgt atagaagccg
      841 tgcttccaac aacaaagttt gtaataaatt ctacccccat ttgtttaagg ttgtttattt
      901 ctatatcaac catataattg gggagtctga attctggtat accatatttt aaaacaccgc
      961 caatttcatg taatgcctca aatacagtaa ctttgcagcc ggctttgatt aaatcgcctg
     1021 cagcagccaa tcctgcaggt cctgaaccta ttactgcaac tttgaatccg ttaggagccg
     1081 caacttcggg tatcgaaatt gttccggact ctcgttcata gtctgctgca aaacgttcta
     1141 aattgccaat tgctacagca ggtttattca tttttatagt atagaaacat tttgattcac
     1201 attgtatttc ttgagggcaa actcttccgc acactgctgg taatgcattt gtttgtttaa
     1261 gaacttttgc agctcctaaa aaatcttttg tttctataca ttttataaat cccggtatat
     1321 ttataccaac agggcatcct tcaacacaag tgggggtaac acaatctaaa caacgttgag
     1381 cttcgtttac agccatttct gctgttaatc ctaagtttac ctcttcatgg tttatgcttc
     1441 gattgtgtgc atcttgttcg ggcatttgta cccgttcttt acttgttctt tcttttgcag
     1501 gaatttgttt acgcaattct gctctccatg caagttcgcg ttgttccttt aaatagtctt
     1561 gatatgtcat aaattggttg tgcttaaact tgttgtaaat acgattgata tgcttctgct
     1621 tcttgatttt tatatgcacc taatcgcata agcatctctt caaaatcaat ttggtgagcg
     1681 tcgaattcgg gaccgtcaac acatacaaat ttggtttgcc cgccaacact tattctacat
     1741 gctccacaca taccggtgcc atctaccatt attgtattta aacttgccac cgtaggaata
     1801 ttatattttt ttgtaagcat tgatgtaaat ttcatcatta tagcagggcc aattgttaca
     1861 cacatgtgaa ctttttcacg ttgtattaca tcttccattc cttgggttac aagtcctttt
     1921 tgcccataag aaccgtcatc ggtcattata attacttcat cagaacaagc tcgtacttct
     1981 tgttccaaaa tcaataattc ttttgtgcgt gcagcaagta ctgaaattac tttgtttccg
     2041 gcagctttca tatcttgaat tataggtaat aatggtgcta taccaacccc tccgcctgca
     2101 caaagtacag taccgaaatt ttctatgtga gttgcttttc ctagagggcc tacaacatcg
     2161 gttatataat caccttcatt aagcatagca agtttatggc tggtaacacc tactttttgt
     2221 agtatcaaag ttatagtacc tttttgtgta tcagcttgag aaattgttag tggaattcgt
     2281 tctccttttt caccaatttt tacaattaca aaatgtcctg ctttgcgttt ttttgctatg
     2341 cggggagctt ctatttcaag ttttactact aggtcagaaa gtatttcttt agtaacaatt
     2401 ttattcattc gtattttttt tacaaatata caattcttag ataaaaaacc tccttactta
     2461 cacaattgaa gcaaggaggt tgtaaaaaaa cataaaaaac ataaaaaaat catattcgtg
     2521 ttaagcacga tttcaatttt ttaaaaatga aagtatgttt gtaaccatat ggcactttat
     2581 tattgtacca tatggtgtat tgtattttta gattactcct tgagctatca ttgcatcggc
     2641 aactttaata aatccaccta tgttagctcc ttttacatag ttaatatagc cgtctttttc
     2701 ggttccgtat tttacacaag tagcatgaat atttttcata atactttgta atttagcatc
     2761 tacctcttca cgagtccatg ataaacgcat agaattttga gtcatttcaa gtcctgatgt
     2821 ggcaactcca ccggcattag cagcttttcc taagccatat aaaattttag cttgaatata
     2881 tacctctatt gcttcaggtg tagatggcat attagctcct tctgatacac atatacatcc
     2941 gtttttaata agagtacgtg catcgtctcc attaatctca ttttgagttg ccgatggtag
     3001 tgctacatca catttaattc cccatggggt ttttccttca tgatattcac atttatattt
     3061 atctgcgtat tcttttattc ttccgcgatt gttgttttta agctccataa taaatgctaa
     3121 tttttctgca tctatacctg caggatcgta aataaatcca tttgagtctg atgctgttac
     3181 acatttagca cctaattgtg tagctttttc aatagcatat tgagctacgt ttccggaacc
     3241 tgaaaccaca caaacttttc ctttcataga atcgccacgt gtttttagca tttcttctgc
     3301 aaaatatact tggccatagc ctgtagcttc cggacgaatt aatgagccac cccatcctat
     3361 acctttgccg gtaagtactc cggtaaattc attgcgtaat cgtttatatt gaccatacat
     3421 ataaccaact tcacgtccgc ctacacctat atcacctgca ggaacgtcgg tgtcagcacc
     3481 aatatgacgt tgtaattctg tcataaaact ttgacaaaaa cgcataactt cattgtctga
     3541 ttttcctttt ggatcaaaat cggaacctcc ctttccacca cccataggta atgttgttaa
     3601 gctatttttg aaaacttgtt caaatgccaa aaatttaagt actcctaaac ttacagtagg
     3661 gtggaaacga atacctcctt tgtatggacc tatagcacta ttcatttgaa ttctgtatcc
     3721 gc
//
LOCUS       DAOTOW010000138         3691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19888_length_3691_cov_2.768152, whole genome shotgun sequence.
ACCESSION   DAOTOW010000138 DAOTOW010000000
VERSION     DAOTOW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19888_length_3691_cov_2.768152"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="P5243_03390"
     CDS             complement(<1..399)
                     /locus_tag="P5243_03390"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HRS18524.1"
                     /translation="MITKGILEFITISKELCAFLELSEYEYKRIFIDKTQKLLSLLYL
                     KALVLNSHDDVDGYCEQFVTEEQWNYIQQSIQTTLGDHDVYIEVTSVENKQSEKDTMP
                     LSEALADVYQDIREFIDRMRTDQERNLSYPE"
     gene            complement(396..1886)
                     /locus_tag="P5243_03395"
     CDS             complement(396..1886)
                     /locus_tag="P5243_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRS18525.1"
                     /translation="MNHYIVAGVIFLYFTLLVVISKITSKTVNNDSFYTGNRNNAWYI
                     VAFGMIGSSISGVTFISVPGWVGNSNFYYYQMIIGNVIGYFIVASLLLPLYYKLQLTS
                     IYTYLEQRFGLYSYKTGASFFILSRTIGSAFRLFIVARVLQIAFFNHFNIPFSVTVII
                     TIALIWLYTYKGGIKTIVWTDTLQTFFLLAAVIITIIIISKSIDLGNSNLIQTIIQSD
                     YSTMFNWDWHSKYNFFKYVLSGMFITIVMTGLDQDMMQKNLSCRSIGDAKKNMYWYST
                     SFLFINLLFLGLGALLYMYVQQVGIQNFSDTSTFHFDASKGMFMKTDDLYPMLATSVF
                     GIAVGIVFLVGIIAAAFSSADSALTALTTSFTIDILGIHNKTEEEIKKQRIITHIGFS
                     ILLALIIIAFHIINNDTVIHAIFTVAGYTYGPILGMYAFGLFTKRSIKDKAMPYIAVA
                     SPLICYGIALFVDYMYSYTVGYEILILNGAITFAGMYIFAQKNKKQ"
     gene            complement(2041..>3691)
                     /locus_tag="P5243_03400"
     CDS             complement(2041..>3691)
                     /locus_tag="P5243_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRS18526.1"
                     /translation="LMKKIVKLKEDNFADKDKYDYAPQDFEDAIDSYEKVVEIVGDLS
                     ANILAPLAESVDHEGPKIVNNEVVYAKGTQIGLEAFRKAELMGMSLPREYGGLNMSTV
                     PYIMAGEIVARADAAFCNIFGLQDCAETVLEFGDEALKKKYLTRVCAGETCAMDLTEP
                     DAGSDLGAVQLKATYDAEKKQWFLNGVKRFITNGDADVNFVLARTEDGTTDARGLSLL
                     MYDRNDKAVIVRRLENKLGIKGSPTCELVFKNAPAELCGDRKMGLIKYIMFLMNGARL
                     GIGAQSVGLCEAAYREALAYAHEREQFGKPIIQFPAVYEMITNMKVRTQAVRSLLYET
                     SRFVDIYKAYDLIAKKRELTAEEKAEARVYSKLADMYTPLTKLFSSEYANSIAYDSLQ
                     VHGGSGFMKDYPIERIYRDARITSIYEGTTQLQVVAAIRGVATGGLLTQIKAYEAEQV
                     EPKFDYLRSILAELTDEYAHCVELVKGTKNTEYLDFHARRLVEMAGNIVMGHLLILDA
                     NRDEEYYDSAEVFIKLIYAENKQKSMYIEKSDVKDLSLYKI"
BASE COUNT         1301 a          689 c          457 g         1244 t
ORIGIN      
        1 ttcaggatag ctcaagtttc tttcttggtc tgttctcatt ctatcaataa actcccggat
       61 atcttggtat acgtctgcta atgcttcaga caggggcatt gtatcttttt ctgattgttt
      121 attttcaacc gatgttactt ctatatatac atcatgatct cccaacgtag tttgaattga
      181 ttgttgaata taattccatt gctcctctgt tacaaactgt tcacaatagc catcaacatc
      241 atcatgtgaa ttcaaaacaa gagctttaag atacagtaaa ctcaacaatt tctgagtttt
      301 atctataaat atacgtttat actcatattc tgacaattct aaaaaagcac ataattcttt
      361 tgaaattgtt ataaattcaa gaattccttt tgtaatcatt gttttttatt tttttgtgca
      421 aatatataca tacctgcaaa agttattgct ccattgagta ttaaaatttc ataccccact
      481 gtatatgaat acatataatc tacaaacaac gctattccat aacaaatcaa aggagacgca
      541 acagctatat aaggcatagc tttatcttta atagaacgtt ttgtaaataa tccaaatgca
      601 tacataccta aaataggccc atatgtataa ccggcaacag taaaaattgc atgtataaca
      661 gtatcattat ttatgatatg aaatgcaata ataataagag ctaacaatat cgaaaaacca
      721 atatgagtta ttattcgttg tttttttatt tcttcttctg ttttattgtg tattcctaaa
      781 atatcaatag taaacgaagt tgttagtgct gtgagagcag aatctgcact tgaaaatgcc
      841 gcggctataa ttcctaccaa aaaaactata cctacagcta ttccaaagac tgatgtagca
      901 agcataggat acaaatcatc tgttttcata aacatacctt ttgaagcatc aaaatgaaaa
      961 gttgacgtgt cagaaaaatt ttgtattcca acttgctgaa catacatata taacaatgct
     1021 cctaacccta aaaacaaaag attaataaat aaaaatgagg tgctatacca atacatattc
     1081 ttttttgcat caccaattga ccgacatgat aaatttttct gcatcatatc ttgatctaac
     1141 ccggtcatta caattgttat aaacataccg gacaatacat atttgaaaaa attatatttt
     1201 gaatgccaat cccaattaaa cattgttgaa taatcacttt gaataatagt ttgtatcaag
     1261 tttgaatttc ccaaatcaat tgatttactt attattatta ttgtaataat aacagctgcc
     1321 aataaaaaaa atgtttgtaa tgtatctgtc cagactattg tttttatacc acctttatac
     1381 gtatacaacc atatcaaggc tatagttata ataactgtaa ccgaaaaagg tatattaaaa
     1441 tgattaaaaa atgctatttg taaaacacgt gcaacaataa ataaacgaaa tgcagaccct
     1501 atagttcgag aaagaataaa aaaagaagct ccggttttat acgaatacaa accaaatctt
     1561 tgttccaaat aagtatatat agatgtgagt tgtaatttat aatataatgg taacaataaa
     1621 gatgcaacaa taaaataccc tattacattt cctattatca tttgataata ataaaaatta
     1681 ctattaccaa cccaaccggg taccgaaata aatgtaactc ccgatatact tgaacctatc
     1741 ataccaaaag ctacaatata ccaagcatta tttctatttc ctgtgtaaaa actatcatta
     1801 tttacggttt ttgatgtaat tttggaaatt acaaccaaca aagtaaaata caagaaaata
     1861 acacccgcaa caatataatg attcatgtta taattataat aaatttaata tccaaatata
     1921 cccttttgta tgaaccttac atatataaat atataaaata tatacacgaa atattgacaa
     1981 aaaaaagcct cgagatgttc gaggcttttt ttattttatt tcaatgtatt cacacattaa
     2041 ttaaattttg tataaactta aatctttaac atctgatttt tcaatataca tagatttttg
     2101 tttgttttca gcataaatta attttataaa cacttcagct gaatcataat attcttcatc
     2161 gcggtttgcg tctaaaatta ataaatgtcc cataactata ttaccagcca tttcaaccaa
     2221 tctacgtgca tggaaatcaa gatattcagt attttttgtt ccttttacta attccacaca
     2281 atgagcatat tcatcggtta actctgctaa aattgaacgt aaataatcaa attttggttc
     2341 tacttgttct gcctcgtatg ctttaatttg agttaacaaa ccacctgttg ccacaccgcg
     2401 aatagcagct acaacttgca attgtgtagt gccttcgtat atagaagtaa tacgagcatc
     2461 acgataaata cgttcaattg gataatcttt cataaatcct gagccaccat gaacttgtaa
     2521 cgaatcatac gctatagaat tagcatattc actcgaaaac aatttggtaa gcggcgtata
     2581 catatcagca agtttgctat atacacgagc ttcagctttt tcttcggcag ttaactcacg
     2641 ttttttagca attaaatcat aggctttata aatatcaaca aatcgagatg tttcatataa
     2701 taatgaacga actgcttgtg tacgaacttt catattcgta atcatttcat acactgcagg
     2761 aaattgaata attggttttc caaactgttc acgttcatga gcatatgcca atgcctcacg
     2821 atacgcagcc tcacataaac caacagattg agctccaata cctaatcgag caccattcat
     2881 aaggaacatt atatatttaa taagtcccat tttacgatcg ccacacaatt ctgcaggtgc
     2941 atttttgaat actaactcac atgttggaga acctttgata cccaatttat tttctaaacg
     3001 acgaacaatt acagctttat catttctatc atacatcaac aatgataaac cacgagcatc
     3061 agttgttcca tcttcggtac gtgctaatac aaaatttaca tcggcatcac catttgtaat
     3121 aaaacgtttt acaccattca agaaccattg ttttttctct gcatcatagg ttgcttttaa
     3181 ttgtacggca cctaaatcag aacctgcatc aggctcagtt aaatccatag cacaagtttc
     3241 acctgcacat acacgtgtta aatatttctt tttaagagct tcgtcaccaa attctaaaac
     3301 cgtttcggca caatcttgta atccaaatat attacaaaac gcagcatctg cacgagcaac
     3361 aatttcacct gccataatgt atggtacagt tgacatattt aaacctccat attcacgagg
     3421 caatgacata cccattaatt ctgctttacg aaatgcttct aaaccaattt gtgttccttt
     3481 agcatacact acttcgttgt ttacaatttt aggaccttcg tggtcaactg attctgctag
     3541 tggagcaaga atgtttgcac ttaaatcccc tactatttca acaacctttt cataactgtc
     3601 tatagcatct tcaaaatcct gaggagcata atcatattta tctttatcag caaagttatc
     3661 ttcttttaac tttacaatct ttttcattaa a
//
LOCUS       DAOTOW010000139         3688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19908_length_3688_cov_3.452794, whole genome shotgun sequence.
ACCESSION   DAOTOW010000139 DAOTOW010000000
VERSION     DAOTOW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19908_length_3688_cov_3.452794"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="P5243_03405"
     CDS             <1..274
                     /locus_tag="P5243_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS18527.1"
                     /translation="ITFTPNSYITATIRCNYISEKLNANSLYPIIDDALICNTKISFN
                     SQQNIQWFVDVRNIFNTEYYHSSNRNVTAYRQPQRTILLGLTWHLE"
     gene            277..810
                     /locus_tag="P5243_03410"
     CDS             277..810
                     /locus_tag="P5243_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HRS18528.1"
                     /translation="MNSTYKYILVLFVAISLWQQTTAQTQEYVIKAVFIERFARFIEW
                     PITSKVSDTEKPFIIGVVGINPFGSELEKLYKNQTIKNKSVIIIYILDYKQIERCDMI
                     YISKSEQTKVPLILEYCQNKPIITISDIEKSLEMGVHIAMGMQQNKVVFAINDKAFEK
                     SQCKVDYRLKQYATQLQ"
     gene            814..2280
                     /locus_tag="P5243_03415"
     CDS             814..2280
                     /locus_tag="P5243_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18529.1"
                     /translation="MTKHIVSIKTKLRRLMLLLVAIELVISLSLHLITDIGKVQNRLQ
                     SQSILYAELLSEYCIAPMTFNDSAAVYDIIKKVEVIPKLLAVAVYTNTEELITLYSKD
                     SLIVIPRKGIENTNNSGIFSKYFTKTQTIVYNNIQLGIICLYVSKQEIKESIINDIRW
                     SFFVSIIIAIVGIILIEIFQRKISNPIIDLLETIKTISSTKNFNIRTQSNTNDEIGVL
                     AEKFNEFIEQIQKKQAAEIIAKQEIENLNKELEERVKQRTLQLEQSNKDLESFAYSVS
                     HDLRAPIRHIDGFTKMLESKLPEKNEQIQHYIDKIHESSKNMQNLISDLLSYSRIGRQ
                     ELQMDMIHIQSVIEEIIETYQQDTQDRSIEWIIHNIPKIYGDIRLIRLAFENLISNAI
                     KYTSKKEHAIIEIGCNITPETIEIFIQDNGVGFDQTFADKLFGVFQRLHSDKDFQGTG
                     IGLANVKRIIQRHGGSVRAEGKENEGATFYVTFPKKKK"
     gene            2343..2777
                     /locus_tag="P5243_03420"
     CDS             2343..2777
                     /locus_tag="P5243_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS18530.1"
                     /translation="MKQTFRILLVEDNENDIELTLEAFTQHGLSENIDICRDGAEALD
                     YLFYKGNFANRTIENPILILLDNKMPKVTGIEVLKIIKQDNVLKNIPVIMLTSSDIDK
                     DIIESYNLGVNAYVVKPVDFEEFIEAVKTLGLFWITLNKVPR"
     gene            2789..>3688
                     /locus_tag="P5243_03425"
     CDS             2789..>3688
                     /locus_tag="P5243_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS18531.1"
                     /translation="MPEQLSIVLLENDDNDVELIQSVLTKSLPQYTIEYKVCETKQDF
                     NAILQTYKPDVILTDFMLPQYNGFMAIEDARKIYLHTPVIIVTGSLSEEDAAESIKHG
                     ASDYVVKQRIERLPIAIMNALKLAKEQEKNHAQFLEIQKLKDQTGLQIKTCFHAIEKA
                     PVAIIITDKLGIIEYANTKFIETTGYALEELVGKTPAILKSGNQTASVYTNMWNEILK
                     GNDWKGEFINKKKNGELFYADVSISSIQDQTGEIKHFIGIQIDVTEEKQKRKELEAAK
                     EKAEESDTLKSLFLANLNHEIRTP"
BASE COUNT         1532 a          540 c          536 g         1080 t
ORIGIN      
        1 aattacattc acacctaatt cttatattac agctacaatt agatgtaatt acataagcga
       61 aaaattaaat gcaaattccc tatatcccat aatagacgat gcgttaattt gcaatactaa
      121 aatcagtttc aattcacaac agaatataca atggtttgta gatgttcgta atatatttaa
      181 taccgaatat tatcattcct caaacagaaa tgttactgca tacagacaac ctcaacgaac
      241 aattctattg ggactaactt ggcatttaga ataaatatga atagtacata caaatatatt
      301 cttgttttgt ttgttgctat aagcctatgg caacaaacaa ctgctcaaac acaagaatat
      361 gtaataaaag ctgtttttat tgaacgtttt gctcgtttta tagaatggcc tattaccagt
      421 aaagtatctg atacagaaaa acctttcata ataggtgtag ttggaataaa tccatttggt
      481 agtgaattag aaaaattata taaaaatcaa actataaaaa ataaatcagt aattataata
      541 tacatattgg attacaaaca aatagaacga tgtgatatga tttatataag taaatcggaa
      601 caaacaaaag tgccacttat attagaatac tgtcaaaata aaccaataat aactatttct
      661 gatattgaaa aatcattaga aatgggtgtg catatagcta tgggaatgca acaaaacaaa
      721 gtagtgtttg caataaacga taaagcattt gaaaaatcac aatgtaaagt agattaccga
      781 ttaaaacaat acgctactca attacaataa atcatgacca aacatatagt gtcaataaaa
      841 acgaaacttc gaagacttat gctgctattg gttgcaatag agcttgttat aagtctatca
      901 ttgcatttga ttaccgatat tggaaaagtt caaaacaggt tgcaatcaca atcaatttta
      961 tatgcagaac ttctatcgga atactgtata gcaccaatga cttttaacga ttctgcagct
     1021 gtttatgata taattaaaaa agttgaagtt attcctaaat tacttgcagt agctgtttat
     1081 acaaataccg aagaactcat aactctatat tccaaagata gtcttattgt aattcctaga
     1141 aagggaattg aaaatacaaa caattcaggc attttttcaa aatattttac aaaaacacaa
     1201 acaatagttt acaataacat acaattagga attatttgtt tatacgtttc aaaacaagaa
     1261 attaaagaat ctataataaa tgatattcgt tggtcatttt ttgtttcaat tattattgct
     1321 atagttggta tcattcttat tgaaattttt cagcgaaaaa tttcgaaccc tattatagat
     1381 ttactagaaa ccataaaaac aataagttcc actaaaaatt ttaatattcg cacacaatca
     1441 aataccaatg atgaaattgg agttttagca gaaaaattta atgaatttat agaacaaata
     1501 caaaaaaaac aagctgcaga aattattgca aaacaagaaa ttgaaaattt aaataaagaa
     1561 ttagaagaac gtgtaaaaca acgcacatta caattagaac aaagcaacaa agatttagaa
     1621 tcatttgcct attcggtttc acatgatttg cgagctccta taagacatat agatggattt
     1681 accaaaatgc ttgaaagtaa acttcccgaa aaaaatgaac aaatccaaca ttatattgat
     1741 aaaattcatg aatcatctaa aaacatgcaa aatttaattt ctgatttgtt atcgtattca
     1801 cgaatcggca gacaagaatt gcaaatggac atgattcata ttcaatctgt gattgaagaa
     1861 attattgaaa cgtatcaaca agacacacaa gacagatcta ttgaatggat aatacataat
     1921 attccaaaaa tatacggaga tattcgctta atacgtttgg cttttgaaaa tttaatttcc
     1981 aatgccataa aatatacatc aaaaaaagaa catgcaataa ttgaaattgg gtgcaacata
     2041 actcctgaaa ctattgaaat atttatacaa gacaatggtg ttggatttga ccaaacattc
     2101 gcagataaac tttttggcgt gtttcaacga ttacatagcg acaaagattt tcaaggaacc
     2161 ggcataggat tggcaaatgt aaaaagaatt attcaaaggc atggcggtag tgtaagagca
     2221 gaaggcaaag aaaatgaagg tgcaacattt tatgtaactt ttcctaaaaa aaagaagtaa
     2281 aaagtattgt aaatactttt aaattaaagt aattttaaac tgtataatat acattaatca
     2341 tcatgaaaca aacatttaga attttactgg tagaagacaa tgaaaacgac attgaactga
     2401 ctttagaagc attcactcaa catggattat cagaaaacat agatatttgt cgtgatggcg
     2461 ccgaagcatt agattatctt ttttataaag gtaattttgc caatagaaca attgaaaatc
     2521 caatccttat tttactcgac aacaaaatgc ccaaagtaac cggtatagaa gtactaaaaa
     2581 ttataaaaca agataacgta ttaaaaaata ttcctgtaat aatgcttaca tcttctgata
     2641 ttgataaaga cataattgaa agttataatt taggagtcaa tgcatacgtt gtaaaacctg
     2701 ttgattttga ggagtttatt gaagcagtga aaacattagg tttattttgg ataacattaa
     2761 acaaagtacc acgctaatac tacatctcat gcccgaacaa ctttcaatag tactacttga
     2821 aaatgatgac aatgatgtag aactcattca gtcggtattg acaaaatcat tacctcaata
     2881 tactattgag tataaagttt gcgaaaccaa acaagatttc aatgcaattc ttcaaacgta
     2941 taaacccgat gtaattctta ctgattttat gctgccacag tataatggat ttatggctat
     3001 agaagatgct cgaaaaatat atttacatac tcccgtaatt attgtaacag gttcattatc
     3061 agaagaagat gcggcagaat caattaaaca tggagcaagc gactatgttg taaaacaacg
     3121 catagaacga ttgccaattg ctataatgaa cgccttaaaa ttggccaaag aacaagaaaa
     3181 aaatcacgca caatttcttg aaattcaaaa actaaaagat caaaccggat tgcaaataaa
     3241 aacatgtttt catgctatag aaaaagctcc ggtagcaata attataactg acaaacttgg
     3301 tataatagaa tatgccaaca ctaaatttat tgaaacaacc ggatatgcat tagaagaatt
     3361 ggtaggcaaa acacctgcaa tattaaaatc gggaaaccaa acagcatcag tgtataccaa
     3421 catgtggaat gaaattctca aaggaaacga ttggaaagga gaattcataa ataaaaagaa
     3481 aaacggagaa ttattttatg ctgatgtttc aatatcgtct atacaagacc aaacaggtga
     3541 aattaaacac tttataggca tacaaataga tgtaaccgaa gaaaaacaaa aaagaaaaga
     3601 attagaagca gccaaagaaa aagcagaaga aagtgatact ttaaaatcat tatttttagc
     3661 aaatttgaac catgaaatac gaactcca
//
LOCUS       DAOTOW010000140         3680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_19971_length_3680_cov_3.319724, whole genome shotgun sequence.
ACCESSION   DAOTOW010000140 DAOTOW010000000
VERSION     DAOTOW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19971_length_3680_cov_3.319724"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..500
                     /gene="dut"
                     /locus_tag="P5243_03430"
     CDS             66..500
                     /gene="dut"
                     /locus_tag="P5243_03430"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRS18532.1"
                     /translation="MIVKIVNKSKHALPNYATEHSAGMDLRANLDAPVVLGPLERKII
                     PTGLFIELPQGYEAQIRPRSGLAAKHGITCLNTPGTIDADYRGEIGVILANVSNEPFT
                     VNDGERICQMVIAQYTKANLVEVQELSKTDRGAGGFGSTGRK"
     gene            538..885
                     /locus_tag="P5243_03435"
     CDS             538..885
                     /locus_tag="P5243_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS18533.1"
                     /translation="MKNNIIKDKSFAFALRIIALYSQLQENKEFVISKQLLRCGTSIG
                     ANINEAIAAESRADFIHKMAIASKEARESLYWLELLHASNLKDFDYTLYIKECTEIVK
                     ILTNIVKSTKSNQ"
     gene            972..1973
                     /locus_tag="P5243_03440"
     CDS             972..1973
                     /locus_tag="P5243_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS18534.1"
                     /translation="MKIIIPMAGMGTRMRPHTLTVPKPLITVCGKPIVHRLVEDITRV
                     CNEVVDEIAFIVHPSFGKVVEQTLVEMAQKLGAKGTICYQAQAQGTAHAVLCAKEALK
                     GNVIVAFADTLFIADFKLNTQQDAIIWVQQVADPSAFGVVKLNGDGLITDFVEKPKEY
                     VSDLAIIGIYYFKDGENLCKELQYLIDNDIHVKGEFQLTTALENMKNKGVKFYPGKVD
                     EWLDCGNKNATVNTNTRLLQNYGSQISKQIIVENSTIIEPCCIADGVTIRNSVVGPYV
                     SVGEGTFISGSVIDTTIVQSNSIIEYTTIKNSMIGTKAIVKTKPLDVSLSDYSEIVQ"
     gene            1973..>3680
                     /locus_tag="P5243_03445"
     CDS             1973..>3680
                     /locus_tag="P5243_03445"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18535.1"
                     /translation="MKCNNFCFYIVLLLCINCLIPVSVFAQKSSKRAQIDSVKQELKQ
                     IEFERILIQANLCKNKKDFEQAIELYHNALSINPLSALVNYELASIYKQKNKYQDALK
                     YAQKAIKLNPSLLEYRYFLAELYDEKGEYIKQIEQLKYILRLQPQNIHLYYTIADIYS
                     NYSEYDKSLQVLQQIQNVFGINEEIRYRRFQIYNKQNKHKQAEEIIQELLQLYPSKVD
                     YVISAIQFYENNALYSDAIRLCNTYINVTNNPSEILYAKARIYAKLDNVDSLAVTLND
                     IFSTTYFNQNQKYDILNRLVLQNVQSKAIYEKRYEIVEKVYFYVPDNEVVNTILARYY
                     LQNNNSQKALPHLYYAIKYEKSNSSLYTQIIELEIQNKQWDSVISVASAGIILFPNSA
                     DLYAALGQAYFQTKQYNKAIIALQEGIDVVLIKNQKRLFYILLGESLYAQKSVETANQ
                     CFDKALQIDSTNMHTICTYAYYAALQNNNVKTVELLNVCNRQCNSNECRYAKAFVLYK
                     QEKYAEAIEMLQSIDISAYTDSYYELLGDVLFKNNNIDEAQKAWNKANEFGATIDIQR
                     KKV"
BASE COUNT         1390 a          504 c          632 g         1154 t
ORIGIN      
        1 gcctaattgc aaatcaaaat aaaaaaacat tgtattttca cgttttattt gaaatatgta
       61 aatatatgat agttaaaata gtaaacaaat caaaacacgc tctaccgaac tatgctacag
      121 aacacagtgc cggaatggat ttacgagcaa atctcgatgc tcctgtagtt ttaggtccac
      181 ttgagcgaaa aattattcca accggtttat ttattgaatt accccaaggg tacgaagcac
      241 aaattcgtcc gcgaagtggt ttggctgcta agcatggaat tacatgcctt aacactcccg
      301 gaactatcga tgccgattac cgtggcgaaa taggtgtaat tttggcgaat gtttctaatg
      361 aaccttttac cgtgaacgat ggagaacgca tatgtcaaat ggtgatagca caatatacaa
      421 aagcaaatct cgtagaagta caagaactaa gcaaaaccga cagaggagcg ggaggatttg
      481 ggagtactgg gcgcaagtaa tttttaatta tgaattttta atttttaatt aaaaagaatg
      541 aagaataata ttataaagga caaaagtttt gcttttgcat taagaataat tgcgttgtat
      601 tctcaattac aagagaataa agaatttgtt atttccaaac aattgctccg atgtggaaca
      661 agcattggtg caaatattaa tgaagcaata gctgctgaaa gtcgagcaga ttttattcac
      721 aaaatggcaa ttgcaagtaa agaagctcgg gaatctttgt attggttaga gttattacat
      781 gcaagtaatt taaaagattt tgactatact ctttatataa aagaatgtac agaaatagtt
      841 aaaattctta caaatattgt aaaatcaaca aaatcaaatc aataataggt tttttaattt
      901 ttgaataagg tactcatggt tcgataagtt taccaatcaa ttaaaaatta aaaattaaaa
      961 attaaaaatt aatgaaaata ataattccaa tggcaggtat gggtactcgt atgagaccac
     1021 atacattaac agttccaaaa cctttgatta cggtgtgtgg taaacctatt gtgcatcggt
     1081 tggtcgaaga tattacacgt gtgtgcaacg aagtggttga cgaaattgcg tttattgttc
     1141 atccttcgtt tggtaaggtt gtagaacaaa cattagtaga aatggctcaa aaactggggg
     1201 ctaaaggtac tatttgttat caggcgcaag cgcaaggtac tgctcatgcg gtgttgtgtg
     1261 ccaaagaagc actcaaaggt aacgtaattg ttgcgtttgc cgataccttg tttattgccg
     1321 atttcaaact caatacgcag caagatgcta taatttgggt gcaacaggtt gcagatcctt
     1381 ctgcctttgg tgttgtaaaa ctcaatggcg atggtttaat taccgatttt gtagaaaaac
     1441 caaaagaata tgtgagcgac cttgctatta ttgggatata ttacttcaaa gatggcgaaa
     1501 atttgtgtaa agaattgcaa tatttgatag acaacgatat tcatgtaaaa ggcgaatttc
     1561 agctcactac cgcgttggaa aacatgaaaa acaaaggcgt taaattttat ccgggcaaag
     1621 ttgatgagtg gcttgattgt ggaaacaaaa atgctaccgt gaataccaat actcgattgt
     1681 tgcaaaatta tggttcgcag atttcgaaac aaataattgt tgaaaattct acaatcatag
     1741 aaccttgttg tattgccgat ggtgttacta ttcgcaactc agtggttggt ccatatgttt
     1801 cggtaggtga aggcacattt atttcgggtt cggtaattga tactacaatt gtgcaatcga
     1861 attctattat tgagtataca acaatcaaaa attctatgat aggaaccaaa gcaatagtaa
     1921 aaacaaaacc gctcgatgtg agtttgagtg attatagtga aatagtgcag taatgaagtg
     1981 taataatttt tgtttttata tagttttatt gctttgtatt aattgtttaa taccggtatc
     2041 tgtatttgca caaaaatcat caaaacgagc acaaatagat tctgtaaaac aagaattaaa
     2101 acaaatagaa tttgaacgaa ttttaataca agcaaattta tgtaaaaaca aaaaagactt
     2161 tgaacaggct attgaattgt atcataatgc attatctatt aatcctttaa gtgctttagt
     2221 gaattatgaa ttagcaagta tttataaaca aaaaaataaa tatcaagatg cactaaaata
     2281 tgctcaaaag gctattaaac ttaatccttc attacttgaa tatagatatt ttttagccga
     2341 attgtatgat gaaaaaggtg agtatattaa gcaaattgaa caactaaaat atatattgcg
     2401 tttgcaacca caaaatatac atttatacta tacaattgcc gatatttatt ctaattatag
     2461 cgaatatgat aaaagtttac aagtattgca gcaaattcaa aatgtatttg gcataaacga
     2521 agaaattcga tacagacgtt ttcaaatata caataagcaa aataaacata aacaagcgga
     2581 agagattata caagaactat tacaattata tccttctaaa gttgattacg taatttctgc
     2641 tattcagttt tatgaaaata atgctctgta ttcagatgca attcgcttat gtaatactta
     2701 tataaatgta acaaataatc catctgaaat actgtatgca aaagctagaa tatacgcaaa
     2761 gttagataat gtagattctt tagctgttac gctaaatgat atttttagta ctacctattt
     2821 taatcaaaat caaaaatatg atattctaaa tcggttagta ttacaaaacg tgcaatcaaa
     2881 agctatatat gaaaaacgat atgaaatagt agaaaaggta tatttttatg ttcctgataa
     2941 tgaggttgta aatacaatac ttgctcgata ttatttacaa aataataatt cacaaaaggc
     3001 attaccacat ttatattatg ctataaagta cgaaaaatca aatagttctc tttatacaca
     3061 aattatagaa cttgaaattc aaaataaaca gtgggatagt gttatatctg tagcgtctgc
     3121 cggtattatt ttatttccta atagtgccga tttatatgct gcgttaggac aagcttattt
     3181 tcaaacaaaa caatacaata aagctataat agcattacaa gaagggatag atgtagtatt
     3241 aataaaaaat caaaaacgct tattttatat tttattgggc gaatcactgt atgctcaaaa
     3301 gtcggtagaa acagcaaatc aatgctttga taaagcatta caaatagact caacaaatat
     3361 gcatactata tgtacgtatg catattatgc cgcattacaa aataataatg taaaaacagt
     3421 tgaattatta aatgtatgta atcgtcaatg taattctaat gaatgtagat atgcaaaagc
     3481 ttttgttttg tataaacaag aaaaatatgc tgaagctata gaaatgcttc aaagtataga
     3541 tatatcagca tatacagatt cttattatga attgctggga gatgtattat ttaagaataa
     3601 taacatagat gaagctcaaa aagcatggaa taaagcaaat gagtttggtg caactattga
     3661 tattcaacga aaaaaagtat
//
LOCUS       DAOTOW010000141         3664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20080_length_3664_cov_2.911610, whole genome shotgun sequence.
ACCESSION   DAOTOW010000141 DAOTOW010000000
VERSION     DAOTOW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20080_length_3664_cov_2.911610"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..785
                     /locus_tag="P5243_03450"
     CDS             246..785
                     /locus_tag="P5243_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS18536.1"
                     /translation="MTNIKVIRATSEHEQYAQAISDLIYQASKEKDAGLAQRAPNYII
                     NKMREGKAVIALAEHEVAGFCYIETWQHGKFVANSGLIVAPKYRGAGLAKRIKQEAFH
                     LSRDLFPHAKLFGLTTSESVMKINSDLGYRPVSYAKLTDDDAFWKGCESCANYDILQR
                     MERKICLCTGMIYDPEEKK"
     gene            898..1422
                     /locus_tag="P5243_03455"
     CDS             898..1422
                     /locus_tag="P5243_03455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRS18537.1"
                     /translation="MRKIRRILCYILLFCIPIVGYGQFPPPPSYNHLFDFYLTPNFPS
                     LVAPDHTFEGSLIRLPNNDILHLFRLDPGYFGNHVGNNGGIGKRISKDNGKTWSAPEV
                     IYNDQYDNRFSTAYRLDNGDIIAFFRRYNCYDTWNGVNVDSDMIISTDNGETWSSRIY
                     LQQIGPSGCFMNVF"
     gene            1723..2772
                     /locus_tag="P5243_03460"
     CDS             1723..2772
                     /locus_tag="P5243_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18538.1"
                     /translation="MYNERFNKALIVVTDRRGASYDMNNFNSGVWVYFEEVDEIMSNP
                     LEYNSYKFLQRGFPNTFRMLGYPSTIETSDTSYIVLYSDAYKKPNSLEDADYYQFSIV
                     INPAIGKKSQSIFLEFMNDFTYEQVPYTPFANATSGLPVRFESTNPSIVKITNNVFEF
                     VGVGECEIIAYQDGNNDFHEAAPQTQTIRVNKAKQNILLPPLQSLRYGQNDISLPPYS
                     TKQLPVSYEITNPDIAEIVDGKLLIKSSGNTELIAWQEGNSLYEPIIKTYLLHIDRAY
                     QDIAIDDIPLLHYKNTYSKLPKYSTVGLELLYESSDTTIAKIKNSELIAVGVGQCELT
                     IQQLGNEIYHPIIKS"
     gene            2824..3282
                     /locus_tag="P5243_03465"
     CDS             2824..3282
                     /locus_tag="P5243_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18539.1"
                     /translation="MNIGDTVIIPTVYASSGLPVSLVSSDTSIAEIKNGKIYPKRHGN
                     CYITATQPGNKLYYAAQDIIQELLVPTIADSVPNVVYTAYPNPSKGVVYISNSQGKRI
                     ELQTLTGETLFLTENLSNEYIDISTFPDGVYVIKIIDNEVYETLFIILQH"
     gene            3403..>3664
                     /locus_tag="P5243_03470"
     CDS             3403..>3664
                     /locus_tag="P5243_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRS18540.1"
                     /translation="MSKIAVLAFSGGLDTSYCAKFYSKEKGLNIHSVIINTGGFSEQE
                     LKDIEHRAHALGVSKHVCIDISHEYYKKFIRYMIYGNILKNNT"
BASE COUNT         1375 a          544 c          559 g         1186 t
ORIGIN      
        1 tgatatatgg attattaatg aagatggtat agctgaattt acgggaaaga aaaaataata
       61 ttctttgtag tatatgtgtc aatttgtaag cattaaatta taatttcgtt caaaattatg
      121 aaacacaaca attttttaaa gaattacaga tataaattca caaaagtata aatcaacaaa
      181 ttaattgaat aagaaaaaaa ttaaccatta tattttttat catttttgca aaaaataact
      241 gtagtatgac aaatattaaa gtaatacgtg ccacatcaga acacgaacaa tatgctcaag
      301 caatttcaga tttaatttat caggcttcaa aagaaaaaga tgccggattg gctcaacgtg
      361 ccccaaatta tataataaac aaaatgcgcg aaggtaaagc tgtaattgct ttagcagaac
      421 atgaagttgc aggattttgt tacatagaaa catggcaaca tggtaaattt gtagcaaatt
      481 ccggattaat agtagcacca aaatatagag gtgcaggatt agcaaaacgg ataaaacaag
      541 aagcatttca tttatcacgt gatttatttc cacatgcaaa actttttgga ctcacaacaa
      601 gtgaatcggt aatgaaaatt aatagtgatt taggatatag accggtaagt tatgcaaaac
      661 taaccgatga tgatgcgttt tggaaaggtt gcgaatcatg tgctaactat gatattcttc
      721 agcggatgga acgtaaaatt tgtttatgta cgggtatgat ttacgacccg gaagaaaaaa
      781 aatgattaac atgtgataaa tactattttt taatgtatat cataaaataa atttatttac
      841 atcatatatt gaaattagtg agtatttttt tgtatattta cattaaataa tataactatg
      901 aggaaaataa gacgaatttt atgttatata ttacttttct gtatccctat tgtaggatat
      961 ggacaatttc ctcctcctcc ttcatataat catttattcg atttttattt aacaccaaat
     1021 tttccaagtt tagttgctcc tgaccacaca tttgaaggtt cattaattcg attacccaat
     1081 aatgatattt tgcatttatt cagattagat cccggttatt ttgggaatca tgttggcaac
     1141 aatggtggaa ttggtaaacg tatttcaaaa gacaatggca aaacatggag tgcacctgaa
     1201 gtaatttata acgatcaata tgacaatcgc ttttcaaccg cttatagact tgacaatggt
     1261 gatattattg ctttttttag acgttataac tgttacgaca catggaacgg agtaaatgta
     1321 gatagtgata tgataataag taccgacaat ggagaaactt ggtcatcacg tatatattta
     1381 caacaaattg gtccttcggg ttgttttatg aatgtatttt aaatagatgg taaaccggga
     1441 tattttgctt caacatatgc taattcatat attgacttac gatatagtat tgacggatat
     1501 tcttgggata gtgtttatta taaatgggac tatagagaca gcgctcattt acaaataaac
     1561 gaaccatatt ttgcctcact tggtggtgga aaagtaattg gtttatttag agtagaaaac
     1621 cgtcctattc accaagtagt atcgtatgat tttggtaaaa cttggtcaaa accaacacca
     1681 acaaatatag caaacggctt tttctgtcct gccccaacta taatgtataa tgaacgattc
     1741 aataaagcat taatagtcgt aacagataga agaggtgcga gttatgatat gaataatttt
     1801 aattcaggag tatgggttta ttttgaagaa gttgatgaaa taatgtcgaa tccattagaa
     1861 tacaactcat ataaattttt acaacgcggt ttcccaaata cattccgaat gttagggtat
     1921 ccttctacca ttgaaaccag cgacacaagt tatatagtat tatatagtga tgcttacaaa
     1981 aaaccaaatt cactggaaga tgccgattat tatcaatttt caattgttat aaatcctgca
     2041 attggcaaaa aatctcaatc tatttttctt gaattcatga acgatttcac atatgaacaa
     2101 gtgccttata ctccttttgc taatgcaact tccggattac ctgttcgatt tgaatctacc
     2161 aatccgtcta tagtaaaaat aaccaacaat gtatttgaat ttgttggcgt tggcgaatgt
     2221 gagattatag catatcaaga cggaaacaac gacttccatg aagccgcacc gcaaactcaa
     2281 actatacgag taaacaaagc aaaacaaaat atactattac ctccattaca atcattacga
     2341 tatggtcaaa atgatatttc acttccacct tattcaacaa aacaattacc agtatcatat
     2401 gaaattacta atcctgacat tgcagaaatt gttgacggta aattgcttat taaatcttcg
     2461 ggtaatacag aattaatagc atggcaagaa ggaaattctt tgtatgaacc aattattaaa
     2521 acatatttac tacacataga tagagcttat caagatatag caattgatga tataccttta
     2581 ttacattata aaaataccta ttctaaatta ccaaaatatt caaccgttgg attagaattg
     2641 ctatatgaat catcagatac tactattgca aaaattaaaa acagtgaatt aatagcagtt
     2701 ggagtaggtc aatgtgaatt aactatacag caattaggca atgaaatata tcatcctatt
     2761 ataaaatcat aaactattat aattgataaa gcaaatcaaa ctatacactt tgaccctata
     2821 attatgaata taggagatac agtaataatt ccgacagtat acgcttcatc aggattacct
     2881 gtttcacttg tttcatcaga cacaagtatt gctgaaatta aaaatggtaa aatttatccg
     2941 aaacgacatg gtaactgtta tattacagca acacaacccg ggaataaatt atattatgca
     3001 gcacaagata taattcaaga acttcttgtg cctactattg cagattctgt tcctaatgtg
     3061 gtatatacag cttaccctaa tccttcaaaa ggagtagtgt atatttcaaa ttcacaaggt
     3121 aaacgcattg aattacaaac tttaacaggt gagaccttat ttttaacaga aaatttatca
     3181 aacgaatata tcgatatctc aacatttcct gatggagtgt atgttattaa aattatagac
     3241 aatgaagtat atgaaacatt atttataatt ttacaacatt agtatttttt ctaaacatat
     3301 gccggtaact tctcaaaata gcaggaaatt attttatttt ttctcaaaaa ctcttacaaa
     3361 aaaagtatat ttgcaacaca aaaaatttat actcaaagaa taatgtcaaa aatagcagta
     3421 ttagcattta gtggaggttt agacacctca tattgtgcaa aattttacag caaagagaaa
     3481 ggattaaata tacattcggt aataatcaat accggaggtt ttagcgaaca agaattaaaa
     3541 gatattgaac acagagctca tgctcttggc gtttcaaaac atgtatgtat tgatatatct
     3601 catgaatatt ataaaaaatt tattcgttat atgatatatg gtaatattct caaaaacaat
     3661 acat
//
LOCUS       DAOTOW010000142         3656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20139_length_3656_cov_3.271591, whole genome shotgun sequence.
ACCESSION   DAOTOW010000142 DAOTOW010000000
VERSION     DAOTOW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20139_length_3656_cov_3.271591"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..908)
                     /gene="coaBC"
                     /locus_tag="P5243_03475"
                     /pseudo
     CDS             complement(1..908)
                     /gene="coaBC"
                     /locus_tag="P5243_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297027.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
     gene            complement(920..1240)
                     /locus_tag="P5243_03480"
     CDS             complement(920..1240)
                     /locus_tag="P5243_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRS18541.1"
                     /translation="MDYKKTKAPNSTITRDLSTLEVGTGNIYETIAIVAKRADQIEVE
                     LKKELDAKLEEFASISDNLEEVFENREQIEISKFYERLPNPVLIALEEFIDKQIYFRK
                     PEVE"
     gene            complement(1260..2060)
                     /gene="bamD"
                     /locus_tag="P5243_03485"
     CDS             complement(1260..2060)
                     /gene="bamD"
                     /locus_tag="P5243_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRS18542.1"
                     /translation="MKVKYILSILAIVVLISCKSEYQKLLTSNDYEKQYNKALEFYEK
                     KEYFKAYELFEKVLPAYRLSEKAEKINYLIARCYYEEGDYLMAAYYFERYTLTFPSSE
                     YAEEAFYFTAISYYNNSPKYSLDQTYTVKAISSFQNYINKYPKGMYIKQSNDYIAQLR
                     EKLEKKAFENSKLFHSIGDYKAAVISLKNCLKDYPDSKYREEIMYLIFESGYFLAQNS
                     IESKKQERMELAMQDYRTYIDEFPQGKWAKDAEKLYVQLQKQTKKAIN"
     gene            2265..2633
                     /locus_tag="P5243_03490"
     CDS             2265..2633
                     /locus_tag="P5243_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRS18543.1"
                     /translation="MKGLLTIGLLVCSNVFMTLAWYGHLKFSQMSWFSKLGLISIIFI
                     SWGIALFEYCFQVPANRIGFKEHGGPFSLVELKVLQEVITLVVFMVFTLIAFKNETFK
                     WNHIVGFVCLILAVYFIFRK"
     gene            2732..>3656
                     /locus_tag="P5243_03495"
     CDS             2732..>3656
                     /locus_tag="P5243_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRS18544.1"
                     /translation="MLIPFLIPFVIGIFLAINMGGSGIGPSFSTAYGANVIRRTAIPG
                     LFGIMVFLGAILGGENTAKTIGNNVLSPQEMTYTIVSIILLSVSISLFIANLLGVPQS
                     TSQSTVMAVTAPALYLNQFNSHTLFVEIIPTWFITPITAFIIAFLVGKYIYKPLRKTG
                     ITISKQVNDSYILKGFILAMAMYVAFSIGANNVANAVGPISVMAINELQVSPKHTTHI
                     LLLSTLIIAPSFAIGSSLLGKRVLQNTGKELVLFGKIEAVIIAFISASLLLLVSLFKG
                     IPTSLVQLNVGAILGIGVAKLGFKNIFSKTEV"
BASE COUNT         1080 a          534 c          623 g         1419 t
ORIGIN      
        1 ggttggttgt aactctattg taagtgattg tttttcttta atttttttgt ctgctactat
       61 ttttggcgta aaatcagcaa cagctgcact caatatggca gcatcacaat ttggaaaatg
      121 gagcatagaa gcttcaaaca tttcttgagc cgaaactaca tcaattcttg taataagggg
      181 atgattaatg tgtaattgtg taggccccga aattagagtt acttctgcac cttgtatagc
      241 tgcttccata gctatagcat atcccatttt tccggtagaa tgattgccta taaaccttac
      301 agggtctatt gcttcatagg ttggtcctgc tgttatgagg attttttttt tttaagtatg
      361 ttttgttccg caaaaaaatc ggataatgca gaaacaattc tttcaggttc tgccattcgg
      421 cctttaccca taagaccact agctaattca ccttcgttag gttctataaa tatattaccg
      481 tatgatgtaa gcgtggcaat attttgttgg gttgcaggat gttgaaacat atccatatcc
      541 atagcaggag ctaatatgac cggacatcga accgaaaggt atgatgtaag taacagattg
      601 tctgctattc cgtgagccat ttttgccata gtattggcag tgcagggagc tacaaggaat
      661 acatcggccc aaagccctaa ttctatatga ctgttccacg ctccgtcatc ataggaaaaa
      721 aaatcgcata atactgtgtt cttacttaaa gttgcaagag ttacaggggt aataaattct
      781 ttagcaaggg gggtcattac aatttttacc tgagcgcctt gtttaattaa taaacgagta
      841 agcattgcag ctttgtaagc cgcaatgctg cctgtaattc ctaacagtat ttttttgcct
      901 tgtaacatag aatatgatgt tattctactt ctggttttct aaagtaaatt tgtttgtcaa
      961 taaattcttc aagcgctatt aatacggggt ttggaagacg ttcgtaaaat ttagagattt
     1021 caatttgttc acgattttca aatacttctt ccaaattatc gcttattgaa gcaaattcct
     1081 ctaatttagc atcgagttct ttttttaact caacttcaat ttgatcagct cgttttgcaa
     1141 ctattgcaat agtttcgtaa atatttcctg ttcctacttc cagagttgat aaatcacgtg
     1201 tgattgtaga atttggagct tttgtttttt tgtaatccat actcaatata tttacatgtt
     1261 taattaattg ctttttttgt ttgtttttgt aattgaacat acagtttttc tgcatcttta
     1321 gcccatttac cttgtggaaa ttcatcaata tatgttctat aatcttgcat tgccaattcc
     1381 atgcgttctt gtttttttga ttctatacta ttttgagcaa gaaaatatcc tgattcaaaa
     1441 ataagataca taatctcttc gcgatattta gagtcaggat agtcttttaa acaatttttt
     1501 aaagaaatta ctgctgcttt gtagtcgcct atgctatgga ataatttgct gttttcaaat
     1561 gctttttttt ctaatttttc acgtaattga gctatataat cattactttg ttttatatac
     1621 atacctttag ggtatttgtt tatgtagttt tgaaaactac tgatagcttt tacagtgtat
     1681 gtttggtcta aactatattt tggtgaattg ttgtaataac ttatggcagt gaaataaaaa
     1741 gcttcttctg catattcact cgaaggaaac gttagcgtat atcgttcaaa ataatatgct
     1801 gccataaggt aatcaccttc ttcataataa cagcgagcta taagataatt aattttttca
     1861 gctttttctg ataatctata tgcaggcaat actttctcaa ataattcata tgccttaaaa
     1921 tattcctttt tttcgtaaaa ttccaatgct ttgttgtatt gtttttcgta gtcgtttgaa
     1981 gttaataatt tctgatattc gcttttgcac gatatcaaaa ccacgattgc aagtatagat
     2041 agtatatatt tcactttcat tttttaatgg gctgcaaatt tagtatattc tgttgtaaaa
     2101 cagtgatttt ttttaacaat ttattacaaa aatgtttttt ttattttttt cctcataacg
     2161 aatggacaaa gattaaattg tttgttgata taatattgaa actatgtaat cacaaaaaca
     2221 gatatgtgtg caattttttt atagtattgc aaaaaaatgt tgatatgaaa ggattactaa
     2281 ctattggatt acttgtatgt tctaatgtat ttatgacatt ggcttggtat ggacatttaa
     2341 aattttcaca aatgagttgg tttagtaaac tagggcttat ttctattatt tttataagtt
     2401 ggggtattgc gttgttcgaa tattgttttc aggttccggc caatagaatt gggttcaaag
     2461 aacatggagg tccattttca ttagtagaac ttaaagtttt acaagaagtt ataacattgg
     2521 tagtttttat ggtgtttact ttgattgctt ttaaaaatga aacttttaaa tggaatcaca
     2581 tagttggctt tgtgtgtctc attttagcag tttattttat ttttaggaaa taaattaacc
     2641 tacgtctttt aacgtatttt ttaaattacg tttttacacg atttttcaag tattttcgat
     2701 tctttacatt tgtcaaaaat ataatgaaag aatgcttata ccttttttga ttccctttgt
     2761 aataggaata tttttagcta tcaatatggg ggggagtgga attggtcctt cattttcaac
     2821 agcttatgga gccaatgtaa ttaggagaac agccattccg ggattatttg gtattatggt
     2881 gtttttaggt gctatattgg gtggagaaaa cactgcaaaa acaataggaa ataatgtgct
     2941 ttcgccgcaa gaaatgacgt atacaattgt ttctattatt ttattatcgg tatctatttc
     3001 attatttata gcaaatttat tgggagttcc tcaatctacc agtcaatcta cggtaatggc
     3061 tgtaactgca ccggcattgt atttaaatca atttaattca catacgctat ttgtggaaat
     3121 tatacctacg tggtttatta cacctattac agcttttatt atagcatttt tggtagggaa
     3181 gtacatttac aaaccgttgc gtaaaacagg gattaccatt tcaaaacagg taaatgatag
     3241 ttatatttta aaaggtttta ttttagcaat ggctatgtat gttgcttttt ctataggtgc
     3301 caacaatgta gcaaatgctg tgggacctat ttcggtaatg gctattaatg aactgcaagt
     3361 tagtcctaaa catacaacac atatattgtt gctttcaacc ttaattattg ctccaagttt
     3421 tgctataggt agttctttat tagggaaacg tgtattacaa aataccggta aagaattagt
     3481 attgtttggt aaaatagaag ctgtaattat tgcatttatt tcagcatcat tgttattatt
     3541 ggtgtcatta tttaaaggta ttccaacttc attggtgcaa cttaatgttg gagctatttt
     3601 aggaataggt gttgctaaat tgggttttaa aaatatattt tcaaaaacag aggtca
//
LOCUS       DAOTOW010000143         3643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20243_length_3643_cov_3.355351, whole genome shotgun sequence.
ACCESSION   DAOTOW010000143 DAOTOW010000000
VERSION     DAOTOW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20243_length_3643_cov_3.355351"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..671
                     /locus_tag="P5243_03500"
     CDS             51..671
                     /locus_tag="P5243_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18545.1"
                     /translation="MGNSYTYNYSVSKELLKPNAIHSLHTYLLENTKAVKYNSNKAEF
                     VVSISTFFFIEEQCIIRYIINKENSNFLISVEYKKSLQFLVFFIIASALFSKFSVSFF
                     LWFCGICMVLFYSIRMVYIHIFISNIKSQITSMYSDEEILSEKQKQWIENPHVCPACG
                     TEINKYTIHCSECGLFIKHLPPVSRFSLSENLDAYNLNYVITDEKD"
     gene            658..1830
                     /locus_tag="P5243_03505"
     CDS             658..1830
                     /locus_tag="P5243_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS18546.1"
                     /translation="MKKIKAHYIFDGSQFHKFGTLVLDDSNRVLEILPESMEEYANTE
                     FYSGIVCPGFVNAHCHLELSHLHNSIPQHTGLPEFISHVVRLRNSVEDREVYMRQADA
                     NMQEQGIVAVCDISNTTESFAVKQSSSIKYHTCIEMFDIFSDTQQVFRDAKQIFSLYK
                     SDLSLSLSLHAPYSCSRILIELLGEHAKEYEYPISIHNQEDASENDFFVSQSGELYTF
                     FKTRNPEMKSFQGKGKSSLQTIAPLLPRKNHILFVHNIFTNYRDVRFLLALRSQRSFT
                     FVICPLSNFYISKQVPPLPLFLEKGISVAIGTDSFASNTKLSILDELIYLQEIFPEIS
                     LEKLLECATKNGAFALGMQRTVGSFVKGAKPGVLLLEQCDLQSLRLTKNTRVHVLV"
     gene            complement(1835..3070)
                     /gene="aroA"
                     /locus_tag="P5243_03510"
     CDS             complement(1835..3070)
                     /gene="aroA"
                     /locus_tag="P5243_03510"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRS18547.1"
                     /translation="MKIIHPSHIQGTIQAPASKSMMQRAIAIATLAHGTSTLHGYTPS
                     NDSDAALKIAHNLGATVTQENTVITIQGGLQPRSTILNCGEAGLGIRMFTPIASLWHK
                     PIELHGEGSLTTRPIDSLIQPLTDLGVHITTNNGFVPVQVCGPMQGGTATVDGSLSSQ
                     ILTGLLIASPLAQKDTIFTVHKLQSKPYIDMTIQIMQDFGITVSHTNYETFSIACNQS
                     YTPQNYVVEGDWSGASFHLVAGAIAGTITITNVRNDSKQADMALVEALRLAGAKIKLH
                     NNSITISKQELNAFHFDATDCPDLFPPLVSLAAHCKGVTTIKGVNRLQHKESNRALVL
                     QTEFAKLGTKIILDADIMYIHGGTLTGGRIHANNDHRIAMAGAVAALLAKQPIEIENP
                     ECVAKSYPHFWEDFTQICK"
     gene            complement(3074..>3643)
                     /locus_tag="P5243_03515"
     CDS             complement(3074..>3643)
                     /locus_tag="P5243_03515"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRS18548.1"
                     /translation="NKPNKDVMRQGLSVPSLALQKHTISTIGLMGAGKTSHGKVLAQK
                     LEYVFKDTDEAIVAKQGIPITQIFEIHGESYFRNLEAQELATSFNSQHPHVLSCGGGI
                     IVNDENRKLLKNNSIVIWLYASPEAIIKRTDISKRPLLQVENPLQKLHEILQARKGMY
                     AKTAHIIVSTERKLKGHTTNKIIKELQAL"
BASE COUNT         1150 a          545 c          616 g         1332 t
ORIGIN      
        1 cagttaaatt ccaaagaccc taatgaagta gatttttaat acttgtaaga atgggcaatt
       61 cttatacata taattattct gtttctaaag aattgcttaa accaaatgca attcacagtt
      121 tacatacata tttattagaa aatacgaaag cggtaaagta taattctaat aaagcagaat
      181 ttgtagttag tatatcaacc ttttttttta ttgaagaaca atgtataata cgatatatta
      241 taaataaaga aaacagtaat tttctaattt cggtagaata taaaaaaagt cttcagttcc
      301 ttgttttttt tataattgct tcggcattat tttcaaaatt ctctgtttct ttttttttat
      361 ggttttgtgg aatatgtatg gttttgtttt attctattcg aatggtatat attcacatat
      421 ttatttctaa tataaaatct caaattactt ctatgtattc tgatgaagaa atattaagtg
      481 aaaagcaaaa acagtggatt gaaaatcctc atgtgtgccc ggcttgtgga actgaaataa
      541 ataagtatac tatacattgt agtgagtgtg gattatttat aaaacacttg ccacctgttt
      601 caagatttag tttatccgaa aatttagatg catataattt gaattatgta attactgatg
      661 aaaaagatta aagcacatta tatatttgat ggtagtcaat ttcataaatt tggaacactt
      721 gtgttagatg attctaatag agtattagaa attttacctg aatctatgga agaatatgca
      781 aatactgaat tttattcagg tattgtatgc cccggttttg taaatgcaca ttgtcattta
      841 gaattatctc atttacataa ctctattcct caacacaccg gattgcctga atttatttcg
      901 catgtggttc gattaagaaa ttcggtagaa gatagagaag tatatatgcg gcaagctgat
      961 gcaaatatgc aagagcaagg tattgttgca gtatgcgata tttcaaatac tacagaaagt
     1021 tttgctgtaa aacaatcatc gtctattaaa tatcatacat gtatagaaat gtttgatata
     1081 ttttctgata ctcaacaagt atttagagat gcaaaacaaa tattttcatt gtataagtct
     1141 gatttatctt tatcattaag ccttcatgcg ccgtattctt gttctagaat tcttatagaa
     1201 ttgctcggtg aacatgcaaa agagtatgaa tatcctatat ctattcataa tcaagaagat
     1261 gcttcagaaa atgatttttt tgtatcgcaa tcgggtgaat tatatacctt ttttaaaaca
     1321 cgtaacccgg agatgaaaag ttttcagggc aaaggtaaaa gttctttgca aactatagca
     1381 ccgttattac cgcgtaaaaa tcatatatta tttgttcata atatttttac aaattataga
     1441 gatgttcgtt ttttacttgc gttacgatca cagcgaagtt ttacgtttgt aatttgtccg
     1501 ttgagtaatt tttatatttc aaagcaagtt ccacctttgc cacttttttt agaaaaaggt
     1561 atttctgtag ctataggaac cgatagtttt gcttcaaata ctaagttatc aatactagat
     1621 gaacttatat atttacaaga aatatttcct gaaatatcgc ttgaaaaact tcttgaatgt
     1681 gctacaaaaa atggtgcatt tgctttggga atgcagagaa ctgtgggtag ttttgttaag
     1741 ggagcaaagc ccggagtatt acttttagaa caatgtgatt tacaatcgtt acgactcaca
     1801 aaaaatacaa gagtacatgt gttagtgtaa aaacttattt gcagatttgc gtaaaatctt
     1861 cccaaaagtg gggatatgat ttagcaacac attccgggtt ttcgatttca ataggctgtt
     1921 ttgccaataa tgctgctaca gctcctgcca ttgcaattct atgatcattg tttgcatgaa
     1981 ttctacctcc tgtaagggtt ccgccatgta tatacatgat atctgcatct aagattattt
     2041 tagtacctaa ttttgcaaat tcagtttgta aaactaaagc acgattgctt tctttatgtt
     2101 gtaacctatt tacacctttg attgtagtaa cacctttgca gtgagctgca agagaaacta
     2161 aaggaggaaa caaatcaggg caatcggtag catcaaaatg aaatgcatta agttcttgtt
     2221 tagaaatagt tatagaatta ttgtgtaatt taatttttgc accggcaaga cgaagagctt
     2281 caactaatgc catatctgcc tgttttgaat catttcttac atttgttatt gtaattgtac
     2341 cagctattgc tcctgcaaca aggtgaaatg aagctccact ccaatcacct tcaactacat
     2401 aattttgagg tgtatatgat tggttacatg caattgaaaa tgtttcataa tttgtatgag
     2461 atactgttat gccaaaatct tgcataattt gaatagtcat atcaatgtat ggtttacttt
     2521 gcaatttatg tacggtaaaa attgtatctt tttgagcaag cggacttgca ataagtaatc
     2581 cggtaagaat ttgagagctc agagaaccgt caactgttgc tgtacctcct tgcattgggc
     2641 cacacacttg tacaggcaca aatccattat ttgttgttat atgtactcct aaatcagtta
     2701 aaggttgtat aagagaatca ataggtctgg tagtaagtga tccttcacca tgcaattcta
     2761 ttggtttatg ccataatgag gcaattgggg taaacattcg tatgcctaaa cctgcctctc
     2821 cgcaattaag aattgtagaa cgtggttgta atccaccttg aattgtaatt actgtatttt
     2881 cttgtgtaac ggtagcgcca agattatgtg caattttgag agcagcatcg ctatcattac
     2941 ttggagtata tccatgaagt gttgatgtgc cgtgtgctaa tgtggcaatt gcaatagcac
     3001 gttgcatcat actttttgaa gcaggtgctt gaatagtgcc ttgtatatgt gaagggtgta
     3061 taattttcat atactataat gcttgtaatt ctttaatgat tttattagta gtatgacctt
     3121 ttaatttacg ttcggtactt acaataatat gtgctgtttt tgcatacatt ccttttcgtg
     3181 cttgtaatat ttcatgtaat ttttgcaaag gattttcaac ttgcagtaat ggacgtttac
     3241 ttatgtcggt tcgttttatt attgcttcag gtgaagcata cagccaaatt acaatagaat
     3301 tgttttttag taattttcta ttttcatcat ttacaattat tcctcctcca cacgataaaa
     3361 catggggatg ttgagagtta aaacttgttg ctaattcctg agcttctaaa ttacggaaat
     3421 aagattctcc atgtatttcg aatatttgtg taattggaat accttgttta gcaacaattg
     3481 cttcatcagt atctttaaaa acatattcaa gcttttgggc taaaactttg ccatgacttg
     3541 tttttcctgc acccattaat ccaatagtag aaatcgtatg tttttgcaaa gctaaacttg
     3601 gtactgataa tccttgtcgc attacatctt tgtttggttt att
//
LOCUS       DAOTOW010000144         3636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20289_length_3636_cov_3.856744, whole genome shotgun sequence.
ACCESSION   DAOTOW010000144 DAOTOW010000000
VERSION     DAOTOW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20289_length_3636_cov_3.856744"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="P5243_03520"
     CDS             <1..1753
                     /locus_tag="P5243_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS18549.1"
                     /translation="KGNQSVIFSESYRQQTNTPVITSMREREYSERAIQNAVHIKTDY
                     MINANHSFDWYNAYISMNSFQIRQTTTNNFSYNYDPENNKYDLNYQTRLQSNYQNILV
                     SSLHGKHTVMPHLEILWASQYSVANNQTPERTHFNLDRIQIDSIANVYPDADGSTIEW
                     AHNTDTDLSGFIHAKYKYTYNTLQFEYKVGSMIRNKERTNFLVEYKIRPDGIQVQGKD
                     FNTIDQISWKLANPLQGSVGPLNYDSQELIMGYYGMVTINHRLGSLQTGIRSEHTNQG
                     YFQYYPAMGQDSSGAQIYTDILPSIQCKYTPTNNTNIRVSYFKSINRPGFYEIVPYVI
                     MEEDYTEFGNKKLKRARIHNIDVRYEYFPKKTEQLLVGVFYKQIQDPIEIMYTTVNRR
                     QYGFGPGNVDKAQNIGFETDIIKYFRVFGVKANYTYTLSKITTPKTLYQKNEKGATER
                     ITKNQTRPLVGQSPHTANIALLYKHDSGIDCQLAASYTHDRLIVVSDFYNSDYWMSKT
                     FQLDAAFEKKWKTGWSCFLKVNNLVNTPMYYYIKIIDNENIDLPLQTTLSDKTLVRKD
                     IYSRSFICGIRKNFITN"
     gene            1766..3055
                     /locus_tag="P5243_03525"
     CDS             1766..3055
                     /locus_tag="P5243_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18550.1"
                     /translation="MKTKGLLILLTLLATVFSSCNKDDKKPEDKSRVYPEGAIVWKKD
                     TIVTLTDHYVVPVNKSLFIEEGVQIIMNDTAVRPEFIVLGNLYCYGTAEKPIRFTVAE
                     QYRSNSKKFNRYWGGIICGYDCEEVLLQHTIVEYGGAQTTEESASFQYQLFKTVTGEG
                     VPGFHFCNPEGRFVITNCTFMNNAEDQVYITGGQSIIMNSQFICNGYDGGDAINYKSD
                     CLADLAYNFIYDPNTNGFKLSNSGMLNTQSDLYVYNNTLVNTGWRRPKIKGGSIWLED
                     NIKAHVFNNILFDTRHPIKQDVKKPMDSTCVYTPNFIFASTQAGIDEFVATEKNGVLN
                     GANDKMSTTPGTNNPNFESFTIQSNVDIMAGVNGSSNVPQQYNSTWDFHLKTGSPALT
                     GGNITITPHYNTNGLTIDNVTYKSPAPAAYFGAYGTK"
     gene            3136..>3636
                     /locus_tag="P5243_03530"
     CDS             3136..>3636
                     /locus_tag="P5243_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HRS18551.1"
                     /translation="MKRIALPFLLIAAGLSMCFIMQTTSEPIVVSPVIVSDTTLNDTD
                     DPAIWYNKQNPSKSLILGTDKGDSTGGIFVFDLQGKLQKHLCIYNLQRPNNIDIEYGF
                     VYNKKVIDIAVFTERGTDKIRVISLPDCKFIDNGGIPIFADDTLRSPMGIALYKNKQQ
                     EIFAFVG"
BASE COUNT         1352 a          509 c          589 g         1186 t
ORIGIN      
        1 aaaaggaaat caatcagtta ttttttctga aagttataga cagcaaacaa atactcctgt
       61 aattaccagc atgcgtgaac gagaatattc tgaaagagct attcaaaatg cggtacacat
      121 taaaaccgat tacatgataa atgccaatca ttcatttgat tggtataatg catatatttc
      181 tatgaattca tttcagattc gccaaacaac tacaaacaat ttttcataca attacgatcc
      241 tgaaaacaat aaatatgatt taaattatca aacacgatta caatcaaatt atcagaatat
      301 tttagtgtca tcattgcatg gcaaacatac tgtaatgcct catttagaaa tattatgggc
      361 aagtcaatat tcagtagcaa ataatcaaac accggagaga actcatttta atttagatag
      421 aattcaaata gacagcatag caaatgtgta tcctgatgct gacggttcta caatagaatg
      481 ggcacataat actgatactg atttatcggg ttttattcat gctaaatata agtatacgta
      541 taatacttta caatttgagt acaaagttgg ttctatgatt cgaaacaaag aacgaacaaa
      601 ctttttggtt gaatataaaa tcagaccaga tggtattcaa gttcaaggaa aagattttaa
      661 tactatagat caaatttcat ggaaattagc aaatcctctt caaggtagtg taggaccatt
      721 gaattatgat tctcaagaat taataatggg gtattatggc atggttacta tcaatcatcg
      781 gttaggaagt ttacaaacgg gtatacgaag tgaacataca aatcaagggt attttcaata
      841 ttatcctgca atgggacaag attcatcagg agctcaaata tatactgata ttttaccttc
      901 tattcaatgt aaatatactc ctacaaacaa tacaaatatt agagtttcat attttaaatc
      961 aattaacaga cccggatttt atgaaatagt tccttatgta ataatggaag aagattatac
     1021 tgaatttgga aataaaaaat taaaacgtgc tcgtattcat aatattgatg ttcgttacga
     1081 gtactttcct aaaaaaacag aacaactatt ggttggagtt ttttataaac aaattcaaga
     1141 tcctattgaa ataatgtaca ctacagtaaa tcgtcgtcaa tatggatttg gtccgggtaa
     1201 tgtagataaa gcacaaaata taggatttga aacagatata ataaaatatt ttagagtttt
     1261 tggtgtaaaa gctaattaca cgtatacatt atcaaaaatt actacaccca aaacgttgta
     1321 tcaaaaaaat gaaaaaggag caaccgaacg tattaccaaa aatcaaacac gtccattagt
     1381 ggggcagtca ccacatacag caaatatagc attgctgtat aaacatgatt cgggtattga
     1441 ttgtcaatta gcagcatcct atactcacga tagacttata gtagtatccg atttttataa
     1501 ttctgattat tggatgtcta aaacctttca gttagatgct gcatttgaaa aaaaatggaa
     1561 aacgggatgg tcatgttttc ttaaggtaaa taatttagtt aatactccta tgtattacta
     1621 tattaaaatt attgataatg agaatattga cttgccattg caaactactt taagtgataa
     1681 aaccttggtt cgtaaggata tttacagtcg ctcttttata tgtggaattc gaaaaaattt
     1741 tattactaat taaatattta tgattatgaa aacaaaaggc ttattaattt tattaacatt
     1801 gttggcaaca gtattttcga gttgtaacaa agatgacaaa aaacccgaag ataaatcgag
     1861 agtttatcct gaaggtgcta ttgtatggaa aaaagataca atagttacat taacagatca
     1921 ctatgttgta ccggtaaata aatccttatt tattgaagaa ggtgtgcaaa ttattatgaa
     1981 tgacactgct gttcgtcctg aattcatagt attaggtaat ctttattgct atggtactgc
     2041 tgaaaaacca attcgtttta ccgtagcaga acaataccgt tctaatagca aaaaattcaa
     2101 tcgctattgg ggtggaatta tttgtggtta cgactgtgaa gaggtattgt tacaacatac
     2161 aattgttgaa tacggaggag ctcaaactac tgaagagtca gcttcattcc aatatcaatt
     2221 atttaaaact gtaacaggag aaggcgttcc cggattccat ttctgtaatc ccgaaggtag
     2281 atttgtaatt acaaattgta catttatgaa taatgcagaa gatcaggtat atattaccgg
     2341 aggtcaatct attattatga attctcaatt tatttgtaat ggttatgacg gaggcgatgc
     2401 tattaattat aaatcagatt gtttagcaga tttagcttat aactttatat atgatcctaa
     2461 cacaaatgga tttaaactat ctaatagtgg tatgttaaat acacaatcag atttatatgt
     2521 atataataat actttggtaa atacaggttg gagacgtcca aaaattaaag gtggcagcat
     2581 ttggttagaa gataatatta aagcgcatgt atttaataat attttatttg atactcgtca
     2641 tccgataaaa caagatgtaa aaaaaccaat ggattcaaca tgtgtatata cacctaattt
     2701 tatttttgca tcaactcaag ccggtataga tgaatttgtt gcaactgaaa aaaatggtgt
     2761 tttaaatgga gctaatgata aaatgagtac tacaccagga acaaataatc caaattttga
     2821 aagttttaca atccaatcta atgtagatat tatggcagga gttaatggtt cttcaaatgt
     2881 tccacaacaa tataattcta catgggattt tcatcttaaa acaggttctc ctgcacttac
     2941 aggtggtaat ataacaatta cacctcatta caatacaaat ggtttaacaa ttgacaatgt
     3001 tacatacaaa tctcctgcac ctgctgcata ttttggtgca tatggaacta aataattttc
     3061 gtaataaata gtaagtttgt aaatatgaag gtgtgttttg aaataataaa cacaccttct
     3121 tttaaaaaaa gtaatatgaa aagaatagca ttaccatttc tattaattgc tgcaggttta
     3181 agtatgtgtt ttattatgca aactactagt gagccaattg ttgtatctcc ggtgattgtt
     3241 tcggatacta cacttaatga taccgacgat ccggcaattt ggtataataa acaaaatcct
     3301 tcaaaaagtt taatattagg aacagacaaa ggtgatagta ccggaggaat ttttgtgttt
     3361 gatttacaag gaaaattaca aaaacactta tgtatatata atttacaaag accaaacaat
     3421 attgatatag aatatggttt tgtttataat aaaaaagtaa ttgatatagc tgtttttaca
     3481 gaacgaggca ctgataaaat tcgtgtaatt tctttgcccg actgtaaatt tattgataat
     3541 ggtggtatac cgatttttgc cgatgatacg ttacgaagtc caatgggtat tgcattatat
     3601 aaaaacaaac aacaagaaat ttttgcattt gtaggt
//
LOCUS       DAOTOW010000145         3613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20432_length_3613_cov_3.778808, whole genome shotgun sequence.
ACCESSION   DAOTOW010000145 DAOTOW010000000
VERSION     DAOTOW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20432_length_3613_cov_3.778808"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..881
                     /gene="lptB"
                     /locus_tag="P5243_03535"
     CDS             156..881
                     /gene="lptB"
                     /locus_tag="P5243_03535"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRS18552.1"
                     /translation="MSILKTENIVKKYGSRTVVKGVSIELHQGEIVGLLGPNGAGKTT
                     SFYMIVGLINPNSGRIILDDIDITKEPIYKRARKGIGYLAQEASVFRKLSVEDNIKSI
                     LEMTSMSKEEQHERLETLIQEFSLQKIRKSLGIQLSGGERRRTEIARALAINPKFILL
                     DEPFAGVDPIAVEDIQRIVAALKKKNIGILITDHNVHETLSITDRAYLLYEGDILKSG
                     NAQELADDEIVRKVYLGENFELR"
     gene            complement(878..3115)
                     /locus_tag="P5243_03540"
     CDS             complement(878..3115)
                     /locus_tag="P5243_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18553.1"
                     /translation="MKHTPIIYISFVIIFMYSQLVAQTIDSKGNTVFINEFKDFKGMI
                     KGEPRVQQVLQLDFQSLLLSYQVENLEDFRGQITQYNEALLKNPKDANTLYKRGLLKM
                     SFKDYNGAISDFVKAIDIVPNFTDAYNNKGLAREALQDYRGAIEDFTLAIQINPQFAD
                     AYYNRGRCKHKLQNIKGALEDYMKASELQPNNACVLLNIGAIKYSLKEFALALEDFNK
                     VKELQPKLAKSYANACAAKYMLDDIDGAFIECNKAIELDLFEADSYNNRGLLYIKQQN
                     YSQAIASFTQAIDLQPALAYVYNNRAIAYYNAGDIKSALDDIEKASQLDTSNVDFSYN
                     KGVIYLESKKYAAAIPEFTKAIIANPKLAKAYFNRAYAKYYTKDYAGANADYTKAIEL
                     NPKVQKAYVNRAAARFKMKNYAGAVEDYDKLLENNALNEDLLIARGIIRNYQKEYNKA
                     IDDFTKVITANPQNWRAYFYRGIAKDYLKDDREAIADYTKAIEIYPTYEMYFNRAIAY
                     NDIEEYKLSIQDYDRAIELRPEDTRAYINRGITKKNLESSKSAISDFTLATQVDSTDV
                     IGYYNRAVLKTGKKNYPNAIQDYTKIIEINPTFINAYYNRGVLRRKTGDYEGAMQDFL
                     KVIELDDTYTPAYNNMALIKKEQGEYDAAIELFSEAISYNFYDAKAYYGRGLLRRKTE
                     DKSGGLVDIKTAATMNPKYTLFLFGRGIAKIKTHDIVGEEFQIQLEKPVGIGNPANPV
                     SVICN"
     gene            complement(3119..>3613)
                     /locus_tag="P5243_03545"
     CDS             complement(3119..>3613)
                     /locus_tag="P5243_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18554.1"
                     /translation="SRTAIQDFSKAIELDSLNERAYYNRGIAFVITEKYAEAIQDFLK
                     VTQINPQNISAFYNKGVLENKLLLYSNAVESLSKAIELNTWDASVYAQRAFAYGNVED
                     YPNAIMDYNKVIELQSKNEKAYFYRGLYRIKTNDIEQGCLDLSKAGELGMDEAFSEIR
                     KKCN"
BASE COUNT         1170 a          591 c          508 g         1344 t
ORIGIN      
        1 ttttttcaga gaacattcct tagttttgag catttttacc aaaaaacata aatttgtggt
       61 taaaaataca aaaataagaa tttaaaatag tgcttaaatc tatttttttt taataatagt
      121 ctgtattttt gtcataaatt ttaaaattgc aaactatgtc gatactgaaa acagaaaata
      181 ttgtaaaaaa atatggaagc cgaacggttg taaaaggtgt ttcgatagag ttgcatcaag
      241 gtgaaattgt gggtcttttg ggtcctaatg gtgccggaaa aactacatcg ttttatatga
      301 ttgtagggtt aataaatcca aattccggac gaattatatt agatgatatt gacattacca
      361 aagagcctat ttataaaaga gctcgaaaag gcattggcta tttggctcaa gaagcttcgg
      421 tatttagaaa attgtcggtg gaagacaata taaaatcaat tcttgaaatg acatcgatga
      481 gtaaagaaga acaacacgaa cgattggaaa cgcttattca agagtttagt ttgcaaaaaa
      541 tacgtaaaag tttaggaatt caactttcgg gtggagaacg tcgtcgtaca gaaatagcgc
      601 gtgcattagc aattaatcca aaatttatat tactcgacga accctttgcc ggtgtagacc
      661 caatagcggt agaagatatt caaagaattg tagccgcatt aaagaaaaaa aatatcggaa
      721 ttttgattac cgaccacaat gttcatgaaa cactatcgat tacggataga gcatatcttt
      781 tatacgaagg tgatatttta aaatctggta atgcacaaga acttgccgat gatgaaattg
      841 ttcgaaaagt atatttagga gaaaattttg aattgcgtta attacatata acacttaccg
      901 gatttgccgg atttccaatg ccaacaggtt tttctaattg tatttgaaat tcttcgccaa
      961 caatatcatg agttttaatt tttgctattc ctcttccaaa taaaaataaa gtatacttgg
     1021 gattcatagt tgctgcagtt tttatatcaa ccaaaccacc tgatttatct tcggttttgc
     1081 gtcgtaataa tcctcgacca tagtaagctt tcgcatcata aaaattatat gatatagctt
     1141 cactaaacaa ttcaatagca gcatcgtatt ctccttgttc ttttttgata agcgccatat
     1201 tgttatatgc aggtgtatat gtatcatcta attcaataac ttttaaaaaa tcttgcattg
     1261 caccttcata atctccagtt ttgcgacgca aaactcctct attataatat gcattaataa
     1321 atgttggatt tatttctata atttttgtat aatcttgtat tgcattagga taattctttt
     1381 tacctgtttt caataccgct ctgttgtaat acccaattac atcggtagaa tctacttgag
     1441 tagccaacgt aaaatcagaa attgcacttt tagaactttc cagatttttt ttagttatcc
     1501 ctctatttat ataggctctt gtatcttcag gacgtaattc tatagctcta tcataatctt
     1561 gaatagataa tttatattct tctatatcat tgtatgcaat tgctctatta aaatacattt
     1621 cataggtagg atatatttca attgctttag tataatcagc tattgcttcc ctatcatctt
     1681 ttaaataatc tttcgcaata cctctataaa agtatgctct ccaattttga ggatttgctg
     1741 ttattacttt agtaaaatca tcaatagctt tgttatattc tttttgataa ttacgaataa
     1801 taccgcgtgc tatgagcaaa tcttcattca acgcattgtt ttccagtaat ttatcgtaat
     1861 cttcgactgc tcctgcgtaa tttttcattt taaaacgtgc agcagctcta ttaacataag
     1921 ctttttgaac ttttggattt aattctatag cttttgtata atcagcattg gccccagcat
     1981 aatctttagt ataatattta gcataagctc tattaaaata tgctttagcc aatttgggat
     2041 tcgcaataat tgctttagta aattcgggaa ttgcagcggc atatttttta ctttctaaat
     2101 atattactcc cttattatat gaaaaatcaa catttgaagt atccaattgc gatgcttttt
     2161 caatatcatc aagagctgat tttatatcac ctgcattata atatgcaata gctctgttat
     2221 tataaacata tgccaaagca ggctgcaaat caatagcttg cgtaaaactt gcaatagctt
     2281 gactataatt ttgttgtttt atatataata aacctctgtt attatatgaa tcagcttcaa
     2341 ataaatcaag ttctattgct ttgttgcatt ctataaatgc tccgtctata tcatcaagca
     2401 tatatttagc agcacatgca ttagcatatg attttgctaa cttaggttgt aattctttaa
     2461 ccttattaaa atcttccaat gccaatgcaa attctttgag agaatattta attgctccaa
     2521 tgtttaataa tacacatgca ttatttggtt gtaattctga agctttcata taatcttcta
     2581 aagctccttt tatattctga agtttatgtt tgcaacgacc tctattataa tacgcatcag
     2641 caaactgagg attaatttga atagcaagag taaaatcttc tattgcacct ctatagtctt
     2701 gtaatgcttc gcgagccaat cctttattat tatatgcatc tgtaaaattt ggtacaatat
     2761 ctatagcctt tacaaaatcg gatatagctc cgttataatc tttaaaagac atttttaata
     2821 atcctcgttt atagagtgta ttggcatctt tagggttttt taatagagct tcattatatt
     2881 gtgttatttg ccccctaaaa tcttcaagat tttctacttg atatgataat aataaactct
     2941 gaaaatctaa ttgtaaaacc tgctgcactc gtggttctcc ttttatcatg cctttaaaat
     3001 ctttaaactc attgataaaa acagtatttc ctttactgtc aattgtttga gctaccaatt
     3061 gagaatacat gaatataata acaaaactta tatatataat aggtgtatgt ttcatgtgct
     3121 aattacattt ttttcttatt tcactgaatg cttcgtccat acccaattct ccggctttac
     3181 ttaaatcaag acagccttgt tctatatcat ttgttttaat tctatataga cctctataaa
     3241 aatatgcctt ttcatttttt gactgtaatt caattacttt gttgtaatcc ataatagcat
     3301 taggataatc ttcaacattg ccgtatgcaa atgctctttg agcatataca gaagcatccc
     3361 atgtatttaa ttctattgct tttgataaac tttcaaccgc attagaatac aacaacagtt
     3421 tattttctaa tactccttta ttataaaatg ccgaaatatt ttgtggatta atttgagtta
     3481 cttttaaaaa atcttgaatt gcttcagcat atttttctgt tattacaaaa gcaatacctc
     3541 tattataata cgcgcgttca ttcaaagagt ccaattctat agctttacta aaatcctgaa
     3601 ttgcagttcg aga
//
LOCUS       DAOTOW010000146         3610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20455_length_3610_cov_3.300422, whole genome shotgun sequence.
ACCESSION   DAOTOW010000146 DAOTOW010000000
VERSION     DAOTOW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20455_length_3610_cov_3.300422"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="P5243_03550"
     CDS             <1..616
                     /locus_tag="P5243_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRS18555.1"
                     /translation="LTTIEFAIQELISLQSGEKEAIVTIQEYAIPASYTYVSIPKLDK
                     SAFLLAYIKGYEGYSLLSGNANIFFEGSFVGQSYFETENTQDSLQISLGRDKGIQIER
                     NRIQDFSEKKMMGQKRKQLFSYEILVRNTKQITVPIIIQDQIPISGQQEINVSSVLHP
                     EGTLDSKTGTVTWKFNLDKSITKKLQISYMVEYPKDWKIEGLSR"
     gene            complement(703..1740)
                     /gene="arsB"
                     /locus_tag="P5243_03555"
     CDS             complement(703..1740)
                     /gene="arsB"
                     /locus_tag="P5243_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRS18556.1"
                     /translation="MKESQSMSFFDRYLSIWVAICIVVGIVIGKFLPIIPQLLEQFEY
                     AQVSLPIAILIWIMIFPMMLKIDFGSVVQAVTMPKGLAVTLTVNWLIKPFTMFGIAWV
                     FFYIFLSKVISVDLAQQYIAGAVLLGAAPCTAMVFVWSHLTKGNAAYTLVQVAINDLI
                     ILVAFIPIVGFLLGVSGISIPWNTLLFSVVLFIVIPLLFAFVTRKYVVKRKGLEYFEN
                     VFIKKFSSTTIIGLLLTLIIIFSFQGDVIVSKPYHIALIAIPLIIQTFIIFGIAYIWA
                     YKWKLPHNIAAPAAMIGASNFFELAVAVAIVLFGLDSGATLVTVVGVLVEVPIMLTLV
                     GIANKTKIKFV"
     gene            complement(1737..2162)
                     /locus_tag="P5243_03560"
     CDS             complement(1737..2162)
                     /locus_tag="P5243_03560"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRS18557.1"
                     /translation="MKILILCTGNSCRSQMAHGFLQSFDTRLTVCSAGTQAAGKLNEG
                     AVAAMKEIGIDISHHTSDQVEQYLDEEWDYVITVCGGANEACPAFIGKVKHRLHMGFD
                     DPSHATGTPEFIQSEFRRVRDEIKEGFYSLYVNQIKPQL"
     gene            complement(2174..2503)
                     /locus_tag="P5243_03565"
     CDS             complement(2174..2503)
                     /locus_tag="P5243_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRS18558.1"
                     /translation="MIKKELFDSELQEIAEFGKAISHPARIAILKFLAESKTCISGDI
                     SEHLPLSRTTVSQHVKELKDLGLIHGEIDGLKINYCLCYDTVVKFKNLFDSFFNDIQN
                     NSIQCNC"
     gene            2662..3447
                     /locus_tag="P5243_03570"
     CDS             2662..3447
                     /locus_tag="P5243_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18559.1"
                     /translation="MKRFLLIPIFTVICASLFAQGEIKNATHANFAWNRPIMNYGEQI
                     NRNSAENVYLFYQNQYQDFKHYGGSFEFGSSFYIHPTGFVKGFKAGVLVDFIDFGANY
                     LTYTDSAIQLVTPTNIKTIQKCHDLTARYSLNVGAMTTYSPKKSIYFDLYFKLRPTFA
                     VNYLRAPMWESIYDRIYTNENQSNIFLPNGEPKIHKRTVEETSIGLGLNYSYGINIRV
                     AKVLVGAEIISGKLKYKAGAIIGMDQTVRDQYLKLKLGFFFAE"
BASE COUNT         1368 a          579 c          494 g         1169 t
ORIGIN      
        1 acttactaca attgaatttg ctatccaaga acttattagt ttacaatcag gagaaaaaga
       61 agcaattgta actattcaag aatatgctat accggcttca tatacatatg taagtattcc
      121 taaattagat aaaagtgcat ttttattagc atacatcaaa ggatacgaag gttattcatt
      181 attatcggga aatgcaaaca tattttttga aggttcattt gttggtcaat catattttga
      241 aacagaaaac acgcaagatt ctttacagat ttcattaggc agagacaaag gaatacaaat
      301 tgaaagaaat cgcattcaag actttagtga gaagaaaatg atgggacaaa agcgcaaaca
      361 actattttcg tatgaaattt tagtacgaaa tacaaaacag attactgttc ctattataat
      421 acaagatcaa atacctatct ctggtcaaca agaaataaat gtaagttctg tattgcaccc
      481 cgaaggtaca cttgattcaa aaaccggtac agtaacatgg aagtttaatt tggataaatc
      541 aatcacaaaa aaacttcaaa tttcatatat ggtggaatat cctaaagatt ggaaaataga
      601 gggattgtca agataaattg gtgaattcaa aaaaacaaat cagaacataa aaaaatgttc
      661 tgatttgttt ttttataaag tactgattca ataaaatatt aattacacaa actttatttt
      721 cgttttattt gcaataccca caagagtcaa cataattgga acctcaacca acactccaac
      781 tactgtaacc aatgtagctc ccgaatctaa cccaaacaac actatagcaa ctgctacggc
      841 aagttcaaaa aaattgcttg cacctatcat tgctgcaggt gcggcaatat tatgaggtaa
      901 cttccattta taggcccata tatatgctat accaaatatt ataaaagttt gtattataag
      961 tggaattgct attaaagcta tatgatatgg ttttgataca attacatctc cttgaaatga
     1021 aaaaataatt attagcgtaa gaagtaatcc aataattgtt gtactcgaaa atttcttgat
     1081 aaatacattt tcaaaatact ctaatccttt acgttttaca acatacttac gagtaacaaa
     1141 agcaaataat aatggaataa caataaacaa tactaccgaa aacaataacg tattccatgg
     1201 aattgaaata ccactaacac ccaacaaaaa tccaactatg gggataaaag caactaatat
     1261 aattaaatca ttaattgcaa cttgtaccaa agtatacgca gcatttcctt tggttaaatg
     1321 actccaaaca aaaaccattg cagtacatgg agctgcgcca agcaatacgg caccggctat
     1381 gtactgttgt gctaaatcta ccgagataac tttagataaa aaaatataaa aaaataccca
     1441 agcaatacca aacatggtaa atggtttaat gagccaattt acagtaagtg ttacagcaag
     1501 acctttgggc attgttactg cttgtacaac actaccaaaa tcaattttaa gcatcattgg
     1561 aaaaatcata atccaaataa gaattgctat tggtaatgat acttgagcat attcaaattg
     1621 ttctaataat tgtggtataa taggtagaaa tttacctatt acaataccaa caacaataca
     1681 aatagctacc catatactta aatatctgtc gaaaaatgac atgctttgtg attctttcat
     1741 aattgtggtt tgatttgatt tacatataat gaataaaaac cttcttttat ttcgtcgcgt
     1801 actctacgaa attcactttg tataaattcc ggggttcctg tagcatgaga aggatcgtca
     1861 aaccccatat gtaatctatg ttttactttt ccaataaatg ctggacatgc ttcattagct
     1921 ccgccacata cagtaataac atagtcccat tcttcatcta aatattgctc tacttggtct
     1981 gacgtgtgat gactaatatc aatacctatc tctttcattg cagcaacagc tccttcattt
     2041 agtttaccag cagcttgagt tcctgcagaa caaacggtaa gtcttgtatc aaatgattgt
     2101 aaaaacccat gtgccatttg acttctacaa ctattaccgg tacataaaat taaaattttc
     2161 ataattattg catttaacag ttacattgaa ttgaattatt ttgaatatca ttaaaaaaag
     2221 aatcgaataa atttttgaat tttactacag tatcataaca caagcaataa ttaattttca
     2281 atccatcaat ttcaccatgt attaacccta aatcttttaa ctcttttaca tgttgtgaaa
     2341 cggtagttct actcaaaggc aaatgctccg aaatatcacc ggaaatacac gttttacttt
     2401 cagccaaaaa ttttaaaata gcaatacgag ccgggtgcga tatggctttt ccaaattccg
     2461 caatctcctg taattctgaa tcaaacaatt ctttttttat catataacaa tatttatatt
     2521 tcgtacgacg caaacatacg acataaaatg gtaaaaaaca aataataagt atacataatt
     2581 taaaaaactt tatttgatta gtttttttta atataaatca gtatatttgc ctgatgtgta
     2641 aataatataa aacttgatac tatgaaaaga tttttactta ttcctatttt tacagttata
     2701 tgtgcttcgt tgtttgcaca aggtgaaata aaaaatgcaa cacatgccaa ttttgcttgg
     2761 aatcgtccta ttatgaatta tggagaacaa ataaatcgca attctgcaga aaatgtgtat
     2821 ttattttatc aaaatcaata tcaagatttt aagcattacg gtggttcttt tgagtttggt
     2881 tcctcatttt atattcaccc aacaggattt gtaaaaggat ttaaagcagg tgttttagtt
     2941 gattttatag attttggagc taactattta acatatacag actcggctat tcaattggta
     3001 acacctacta acataaaaac aattcaaaaa tgtcatgacc ttacagcacg atattctctt
     3061 aatgtaggag ctatgactac atatagtcct aaaaaaagta tatactttga cttatatttt
     3121 aaattaagac ctacttttgc agttaattat ttaagagctc ctatgtggga aagtatttat
     3181 gacagaattt ataccaatga aaatcaatct aatatatttt taccgaatgg tgaacccaaa
     3241 atacataaaa gaacagttga agaaaccagt attggattag gacttaatta ttcgtatggt
     3301 ataaatatac gtgttgcaaa agtattagta ggagcagaaa taatttcggg aaaactgaaa
     3361 tataaagccg gtgcaattat tggaatggat caaacagttc gcgaccaata tttaaaactt
     3421 aaattaggat ttttcttcgc agaataaact aaaatcataa taaaaaaata ttataatctc
     3481 caatacttta atgtattgga gatttttatt tttaaacata tacaaaacag aatcttttta
     3541 cgtactttta cattaataaa tctatattga agcatgattc aacatttttt caaaataatt
     3601 gtaataagta
//
LOCUS       DAOTOW010000147         3609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20461_length_3609_cov_3.070343, whole genome shotgun sequence.
ACCESSION   DAOTOW010000147 DAOTOW010000000
VERSION     DAOTOW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20461_length_3609_cov_3.070343"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="P5243_03575"
     CDS             <1..793
                     /locus_tag="P5243_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS18560.1"
                     /translation="GASRNARIDDILTQAQEIAEAGVKEIVLTGINIGDFGKSTGESF
                     LQLLEKLDTVSGIERIRISSIEPNLLTTDIISFVAQSRACMPHFHIPLQAGSNAVLAL
                     MRRRYTTELFQSKISEIVNIIPHAAIGVDVIVGTPGETEELFQESYSFIQSLPISYLH
                     VFTYSERENTDALHIKPRIAPHIRHVRSVLMHELSDKLHALYVRKFNNTVRPVLFESK
                     QKNGMYSGYTDNYIKVAAHIAKTVQNTIVPVMLQCTEKEVIGKEI"
     gene            888..1844
                     /locus_tag="P5243_03580"
     CDS             888..1844
                     /locus_tag="P5243_03580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRS18561.1"
                     /translation="MKLKTCIIPEQFYSLSIVSRIFIGFIFSIIFGLLWFYAFMFIGS
                     IVWRQTMFDFIEQVSISVQYVESKALIQFLLFTQSISIFLIPAIVMSKIIRCDIISQW
                     KLNKFPNIFFMFLIIQIIIINIPGMNFWAGINTYIVELFASKESFIYTSFIQNQKVTE
                     FILKAPCFADLLVNICCIAIVPAITEELFFRGMLQRYATAAIGKVQLAIVLTSIIFSL
                     LHGDLYNFIPRFCMGIIFGYLYWYTNNLWVPIIAHFIHNASVVVSYYVIQHGVFHPNF
                     EKIGMWGNASIFGFVSIIIVAAFMTYMVSNKMHINLSEKEDL"
     gene            complement(1853..3502)
                     /gene="sulP"
                     /locus_tag="P5243_03585"
     CDS             complement(1853..3502)
                     /gene="sulP"
                     /locus_tag="P5243_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HRS18562.1"
                     /translation="MPHIFKPKLFAVLKLGYTKEQFTKDVFAGIIVGIVALPLAIAFA
                     VASGVSPEKGLITAVVAGFIISFLGGSRVQIGGPTGAFIVIVYSVLQQYGYDGLLIST
                     ILAGLLLIIFGILKLGILLKYIPHPLIVGFTSGIALVIFSTQIKDALGLEIEKVPGEF
                     IHKWAIYWEHISTFNPYAIAITLTTILITVYFKKITTKIPGSFVAIICVTVLVQITQA
                     PVSTIESVFGSIPQTISFHIPQVDFSNIQMYIAPALTIALLGGIESLLSAVVSDGMIG
                     SKHQSNTELVAQGIANVVTPFFGGIPATGAIARTATNVKNGARTPIAGMVHAITLLCI
                     MLFVGNWAKLIPMSCLAGILIVVSYNMSEWRSFVNIFRGSAFDILILVVTFLLTVFVD
                     LTVAIEVGVVLASLLFMKRMSDASMLTKETIDKDILENYAYLPKELAIYEISGPFFFG
                     SAKSYCNTLREIGSKAKVLIIRMRHVPFVDATAIHNLQDVIRELQHDGIKIILSGVNE
                     QVYNELEKNRIVFLVGKNNVVSTFDKALQVSYAYLGKEEKM"
BASE COUNT         1238 a          540 c          583 g         1248 t
ORIGIN      
        1 aggtgcaagt agaaatgcaa gaattgacga tatattaact caagctcaag aaatagctga
       61 agcaggagta aaagaaatag ttcttacagg aattaatata ggtgatttcg ggaaatcaac
      121 cggtgaaagt tttttacaat tacttgaaaa acttgataca gtttcgggga tagaacgtat
      181 tcgaatatca tctattgaac caaatttgtt aactacagat attatttctt ttgttgcaca
      241 atcaagagca tgtatgcctc attttcatat ccctttacaa gcaggtagta atgcggtatt
      301 ggctcttatg cgtcgtcggt ataccacaga attatttcaa agcaaaattt ccgaaatagt
      361 aaacattatt ccgcatgctg ctattggagt agacgtaata gttggtacac ccggtgaaac
      421 agaagaattg tttcaagaat catatagttt tatacaatcg ttacctataa gttatttaca
      481 tgtgtttaca tacagcgaaa gagaaaatac cgatgcttta catataaaac ctcgaatagc
      541 acctcatatt cgccatgtta gaagtgttct tatgcatgag ttgtcagata aacttcatgc
      601 tttatatgtg agaaaattca ataatactgt gcgtccggta ttgtttgaat cgaaacaaaa
      661 aaatggtatg tacagtggat ataccgataa ttatataaaa gtagctgcac acatagcaaa
      721 aacagttcaa aatactattg tacctgttat gttacagtgt acagaaaaag aagtaatagg
      781 aaaggaaata taatatgtat tcttttttta tttttactgc atttaatcta aagagtgttt
      841 tgtgtgaaaa aaactaaaat aataaagctc gatttataca gaaaataatg aaattaaaaa
      901 catgtataat accggaacaa ttttattcat tatcaatagt gagtagaata tttatcggtt
      961 ttattttttc aatcattttt ggattactgt ggttttatgc gtttatgttt ataggttcta
     1021 ttgtatggag gcaaactatg tttgatttta ttgaacaagt ttcaatttct gtgcaatatg
     1081 tagaatcaaa agctctcatt cagtttttat tatttactca gtcaatttct atatttttaa
     1141 taccggctat agtaatgtct aaaattatca gatgtgatat tattagtcaa tggaaactaa
     1201 ataaattccc aaatattttt tttatgtttc ttataattca aattataata ataaatatac
     1261 cgggaatgaa tttttgggct ggtataaata catatatagt tgaattattt gcttcaaaag
     1321 aatcatttat ttacacatca tttattcaaa atcagaaagt tacggaattt atacttaaag
     1381 ctccttgttt tgccgatttg cttgtaaata tatgttgtat agcaatagtt cctgctataa
     1441 cagaagaatt attttttaga gggatgttgc aacgctatgc cacggctgct attggaaagg
     1501 tacaattagc aatagtgctt acttcaatta tttttagttt gttgcatggt gatttatata
     1561 attttattcc acgattctgc atgggaatta tatttggtta tttgtattgg tacacaaaca
     1621 atctttgggt accaataata gcacatttta tacataatgc ttcggttgtt gtatcgtatt
     1681 atgtaattca acatggagta tttcatccaa attttgaaaa aattggaatg tggggaaatg
     1741 caagtatttt tggatttgta agtataatta tagttgcagc ttttatgaca tatatggtta
     1801 gcaataaaat gcacataaat ttatcagaaa aagaagattt gtaatgctgt ttttacattt
     1861 tttcttcttt tcctaaataa gcataactta cttgtaatgc tttatcaaat gtactaacta
     1921 cattattttt acctacaaga aacacaattc tgtttttttc taattcatta tatacctgtt
     1981 catttactcc cgataaaatg attttgatac catcgtgttg taattcacga attacatctt
     2041 gtaaattgtg gatagcagta gcatctacaa atggaacatg tcgcatacga ataataagga
     2101 cttttgcttt actgccaatt tcgcggagtg tgttgcagta tgattttgcc gaaccaaaaa
     2161 agaatggacc actaatttca tatatagcta attcttttgg taaataagca taattttcca
     2221 aaatatcttt gtcaatagtt tctttggtaa gcatacttgc atcgctcatg cgtttcataa
     2281 acaataacga agctaatact acacctactt ctatagctac ggttaaatct acaaatacgg
     2341 tgagtaaaaa ggtaacaaca agtataagaa tgtcaaatgc cgaacctcgg aaaatattca
     2401 caaaagatcg ccattcgctc atgttatacg acacaactat aagtataccg gccaaacaac
     2461 tcatagggat aagttttgcc caatttccta caaaaagcat tatgcataat agtgttatgg
     2521 catgtaccat acctgcaata ggagtacgag caccattttt tacattagtt gcagtacgtg
     2581 ctatagctcc tgtggcaggt atacctccaa aaaatggtgt aacaacatta gctattcctt
     2641 gcgctactaa ttcagtatta gactgatgtt tacttccaat cataccatca gatactacag
     2701 cagaaagtaa tgattctata ccaccaagca atgctattgt taaagcagga gctatataca
     2761 tttgaatgtt tgaaaaatct acttgtggaa tatgaaatga tatggtttga ggaatagaac
     2821 caaatacaga ttcaatagta ctaacaggtg cttgtgtgat ttgaactaaa accgttacgc
     2881 atatgatagc aacaaatgat cccggaattt ttgtagtaat ttttttgaaa taaacggtta
     2941 taagtatagt tgtaagagtt atagctatag catatggatt aaatgttgaa atgtgttccc
     3001 aatatattgc ccatttgtgt ataaattctc ccggtacttt ttcaatctct aatcctaatg
     3061 catcttttat ttgagttgag aaaattacca atgctatacc acttgtaaat ccaacaatga
     3121 gagggtgggg aatatatttt aataaaattc ctaattttaa aatgccaaat ataattaata
     3181 agagtccggc taagatagta gaaataagta atccatcata tccatattgt tgtaaaacac
     3241 tgtatactat tacaataaat gctcctgttg gaccaccaat ttgtacacgg cttccgccta
     3301 aaaaagatat aataaatccg gctactaccg ctgttatcaa tcctttttcg ggcgatacac
     3361 ccgatgctac agcaaatgct atagccaatg gtaaagctac aattcctact ataatcccgg
     3421 caaatacatc tttagtaaat tgttcttttg tataccctaa tttgagtact gcaaataatt
     3481 tgggtttaaa tatgtgtggc atgattcaaa aagtctaaaa agtctgaaag attttggttt
     3541 atggtatatg gtttattgtc aagtttcaca aggttgttta tgataactta attgcaaaga
     3601 taaaacttt
//
LOCUS       DAOTOW010000148         3608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20471_length_3608_cov_2.783282, whole genome shotgun sequence.
ACCESSION   DAOTOW010000148 DAOTOW010000000
VERSION     DAOTOW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20471_length_3608_cov_2.783282"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1779
                     /gene="ettA"
                     /locus_tag="P5243_03590"
     CDS             103..1779
                     /gene="ettA"
                     /locus_tag="P5243_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRS18563.1"
                     /translation="MSDDKKVIFSMVKVSKTFPPQKQVLKDIYLSFFYGAKIGIIGLN
                     GSGKSTLLKIIAGIEKNYQGEVVFSKGYSVGYLAQEPELDNTKTVKEIVQEGMQELVD
                     LLKEYEDINLKFMEPMSDDEMTKLIERQGELTELLDQKGAWELDNKLERAMDALRCPD
                     GDESVAHLSGGERRRVALCRLLLQEPDVLLLDEPTNHLDAESIQWLEQHLQQYKGTVI
                     AITHDRYFLDNVAGWILELDRGEGIPWKGNYTSWLDQKTKLMAQEEKQASKRRKTLER
                     ELEWVRMSPKARTTKSKARLSAYDKMLNEDTKEAIEKLEIFIPAGPRLGDKVIEAKDV
                     SKAFGDKILFENLNFSLPPAGIVGVIGPNGAGKTTLFRMIMGQMSPDSGTFVVGDTVK
                     VAYVDQAHEEIDPNKSVFEVITGGNENMEFNGKLFNSRAYVGRFNFSGADQEKKCGVL
                     SGGERNRLHLAMTLKAEANVLLLDEPTNDIDVNTLRALEEGLENFPGCAVVISHDRWF
                     LDRIATHILAFEGNSQVVFFEGSYSDYEENRRKRLGNEGPKRIKYKKLVK"
     gene            1787..1963
                     /locus_tag="P5243_03595"
     CDS             1787..1963
                     /locus_tag="P5243_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HRS18564.1"
                     /translation="MGIFGIIQIICAVWVIIEVYKRTDKTNEQKIIWIVAAVILGIAT
                     AIVYYILEKKDKQI"
     gene            1965..2168
                     /locus_tag="P5243_03600"
     CDS             1965..2168
                     /locus_tag="P5243_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HRS18565.1"
                     /translation="MARLLPILAIAAAIYVLYQIWIVEKNNKSKEEKLLWTLLALLFS
                     VITAGIYYFLEKDKFNNNEKNLN"
     gene            2149..2787
                     /locus_tag="P5243_03605"
     CDS             2149..2787
                     /locus_tag="P5243_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRS18566.1"
                     /translation="MKKILINLLILSTCTFATFAQNSANIQLPAPKKDGGIPIMKALQ
                     NRKSSRTFVAGKELTNQQISNLLWAAYGFNRPNKRTAPSATDSQEFTLYVFLKSGIYK
                     WDDSKNILIHIATGDYRAITGKQDFVKDASLNIVFVANYNKLTKYTTDIEKSNMAHIN
                     CGYISQNIYLYCASEGLATIVRGWIDKDEIAKKLQLPSYQKVIVAQSVGYDK"
     gene            2799..3509
                     /locus_tag="P5243_03610"
     CDS             2799..3509
                     /locus_tag="P5243_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS18567.1"
                     /translation="MNSRIAIVTGANRGLGKALAEKLAQDGIFIILVSRDVKKAEPTL
                     QNIEKQGGKGIIKQCDISKTSDIESFAQEISKEFPCIDILINCAAINIDSQEVTIETM
                     PLELMEQTLATNFYGTLWMCKHIVPLMRASKDGRIINFSSGLGQLGVPRMGPMPAYSM
                     SKTIVNAITKNLAEELKNTNIIVTSVDPGWVLTDMGGPNAMLTIPEGIDTPYWLATAP
                     ANEISSGYFYKERKILEW"
BASE COUNT         1318 a          605 c          666 g         1019 t
ORIGIN      
        1 aaataaattt tgttttatta aattaatttt gtagttttat cggacaataa tgattctcaa
       61 attaatatta tatatacttt tgtttatata aattcaatac atatgagcga cgacaaaaaa
      121 gttatttttt cgatggttaa ggtaagtaaa acctttccac cccaaaaaca agttttaaaa
      181 gatatatatt tatcattttt ttacggtgcc aaaatcggca ttatcggtct caatggttcg
      241 ggaaaatcta cgcttttaaa aattattgca ggtattgaaa agaattacca aggtgaagtt
      301 gttttttcaa aaggatatag cgttggatat ttggcccaag aacccgaatt ggacaatacc
      361 aaaacggtaa aagaaattgt gcaagaagga atgcaagaat tagttgattt gctcaaagaa
      421 tacgaagaca taaatctgaa atttatggaa cccatgagcg acgatgagat gacaaaactt
      481 attgaacgcc aaggagaatt aactgaatta ttagaccaaa aaggtgcatg ggaacttgac
      541 aataaacttg aacgagcaat ggatgcgttg cgttgccccg atggcgatga atctgttgca
      601 catttatccg gtggtgaacg aagacgagta gcgttatgca gactattgtt acaagaaccc
      661 gatgtactgt tgctcgacga gccaaccaac caccttgatg cagaatcaat tcaatggctt
      721 gaacaacact tacaacaata taaaggtacg gtaatagcaa tcactcacga ccgctatttc
      781 ttagacaatg tcgcaggctg gattttggaa ctcgaccgtg gcgaaggtat tccatggaaa
      841 ggaaattaca catcgtggct tgaccaaaaa actaaactga tggctcagga agaaaaacaa
      901 gccagcaaac gtcgtaaaac cctggaacgc gagcttgaat gggtacgaat gtcaccaaaa
      961 gctcgtacta caaaatcaaa agcacgcctc tctgcatatg ataaaatgct taacgaagat
     1021 acaaaagaag caatagaaaa acttgaaatt ttcatacctg ccggtcctcg tttaggtgat
     1081 aaggtaatag aagcaaaaga tgtatccaaa gcttttggtg ataaaatttt atttgagaat
     1141 ctcaacttta gtcttccacc tgcaggtata gttggtgtaa ttggaccaaa cggtgccggc
     1201 aaaacaactc tgttccgcat gattatgggg caaatgtctc ccgattcagg tacatttgtg
     1261 gttggcgata ccgtaaaagt tgcttatgta gatcaagcac atgaagaaat tgaccctaac
     1321 aaatcggttt ttgaagtaat taccggaggc aatgaaaaca tggaattcaa cggtaaatta
     1381 ttcaattcac gtgcatatgt gggacggttt aacttcagcg gtgccgacca agaaaaaaaa
     1441 tgtggcgtac tttcgggtgg agaacgcaat cggttacact tagctatgac actaaaagca
     1501 gaagctaacg tgcttcttct cgatgagcct accaatgata tagatgtaaa cacactccgc
     1561 gcactagaag aaggtttaga aaacttcccc ggatgtgctg tagttatatc acacgaccga
     1621 tggtttctcg accgtattgc aacacacatt cttgcattcg aaggcaattc acaagtagta
     1681 ttcttcgaag gcagttattc cgattacgaa gaaaatcgta gaaaacgatt aggtaacgaa
     1741 ggtccaaaac gtattaaata taaaaaactt gtaaaataat aatagtatgg gaatttttgg
     1801 tataattcaa attatttgtg cggtatgggt tattatagag gtatataaac gcaccgataa
     1861 aacaaatgaa caaaaaatta tatggattgt agcggctgtt attcttggaa ttgcaacagc
     1921 tattgtatat tatattttag aaaaaaaaga taaacaaata taacatggca cgattactcc
     1981 ctattctcgc tattgcagca gctatctatg tgttatatca aatatggata gtagaaaaaa
     2041 acaataaatc taaagaagaa aaacttttgt ggacgttgct tgccctccta tttagtgtta
     2101 ttacggcagg tatttattat tttttggaaa aagacaaatt caataataat gaaaaaaatc
     2161 ttaattaatc tgttgatttt gagcacatgt acttttgcta cattcgcaca aaattctgca
     2221 aatattcaat tacctgcacc taaaaaagat ggcgggatac ccataatgaa agccttgcaa
     2281 aacagaaaaa gttcacgaac atttgttgca ggaaaagaac tcacgaacca acaaatttca
     2341 aatttactgt gggctgcata tggttttaac cgtcccaaca aacgaacagc tccgtctgca
     2401 accgattctc aagaatttac tttatatgtt tttttgaaat cgggtattta caaatgggac
     2461 gattctaaaa acatattaat acacattgct accggtgact atagagcaat aaccgggaaa
     2521 caagattttg taaaagacgc atcactcaat attgtttttg tagccaatta caataaactt
     2581 acaaaatata caactgatat tgaaaaatca aatatggcac atataaactg tggctatatt
     2641 tctcaaaata tatatcttta ttgtgcatca gaaggattag ctacaattgt gagaggatgg
     2701 attgataaag atgaaattgc taaaaaatta caattacctt catatcaaaa agtaattgta
     2761 gcacaatcag taggatacga taaataaaat atacatatat gaattcaaga attgcaattg
     2821 ttacaggagc taacagagga ttaggtaaag cattagcaga aaaattagca caagacggca
     2881 tttttataat attggtaagc cgtgatgtaa aaaaagcaga accaacatta caaaacattg
     2941 aaaaacaagg aggtaaagga attatcaaac aatgtgacat ttcaaaaact tctgatattg
     3001 aatcgtttgc acaagaaatt tcaaaagaat ttccttgtat agacatactt ataaactgtg
     3061 ctgcgataaa tattgacagt caagaagtaa ctatagaaac catgcctttg gaacttatgg
     3121 aacaaacact tgctacaaat ttttatggta cattgtggat gtgtaaacat atagtacctc
     3181 taatgcgagc atcaaaagac ggaagaatta taaacttttc atccgggtta ggtcaacttg
     3241 gagttccccg tatgggacca atgccggcat atagcatgag caaaacaata gtaaacgcta
     3301 ttactaaaaa tttagcagaa gaactaaaaa ataccaatat cattgtaact tctgttgacc
     3361 ctgggtgggt acttactgac atgggtggcc ccaatgctat gcttacaata ccagaaggta
     3421 ttgatactcc ctattggtta gcaacagctc ctgccaatga aatatcatcg ggctatttct
     3481 acaaagaacg aaaaatttta gaatggtaaa ttttaattat gaattatgag atatgaatta
     3541 aaaaattaat aaactgttta gtttcaccac gttgttaatg gaaaaacgtc tcccccttcc
     3601 tttgcttc
//
LOCUS       DAOTOW010000149         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20549_length_3598_cov_4.116286, whole genome shotgun sequence.
ACCESSION   DAOTOW010000149 DAOTOW010000000
VERSION     DAOTOW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20549_length_3598_cov_4.116286"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="P5243_03615"
     CDS             complement(<1..521)
                     /locus_tag="P5243_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRS18568.1"
                     /translation="MKNTKTWIIIGGIVILALYLISQYNSLVTMQESVKKQWANVESS
                     YQRRADLIPNLVKTVKGYADFEKSTLQSVIEARAKATGVTVNAENLTPETIAKFQEAQ
                     NQMSGALSRLLVVVEKYPDLKANQNFLELQAQLEGTENRINVERNRYNEMVQPFNTKI
                     KTFPTNLMNALYG"
     gene            complement(526..1632)
                     /locus_tag="P5243_03620"
     CDS             complement(526..1632)
                     /locus_tag="P5243_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRS18569.1"
                     /translation="MKFVRFILFCVNLIASLLLVFTAIARYINPQLIWMFEFVALAYP
                     VYLLVNLFFVIFWLFTKHKLNVLVSVIGFVLSISTFFDTFSFAGSPKHDVSGDTVKII
                     SYNVNAFNYNGWRGRAGIQAQVFDFLKQESPDIICFQEFHHDTHEKYQMIDSIKKQLG
                     LRYIQIHRVHEIKGRYFYGNVIFSKFPIVKTGVILYQKTGNTTLWADIKLYNDTVRIY
                     NNHLESYKLQPENIETIEMTSTGQEFESQKLRKVGGKLKRAIQKRGKQTQELLEAMRE
                     NPYETIVCGDFNAPPYSFTYKAIRSEKDLQDAFLEASYGLGGTLNWRLLSKRLDYVLL
                     PQKVIVQEFTQQKLPISDHFPISVSFIIQNNINN"
     gene            complement(1636..2535)
                     /locus_tag="P5243_03625"
     CDS             complement(1636..2535)
                     /locus_tag="P5243_03625"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS18570.1"
                     /translation="MTIWHDIQREFREGNNLIKIIFINIAVFLILNILQVLSWIFTGQ
                     QESGIYTFIISNIQTYISVPKLIVKPWTLVTYMFTHVDFLHILFNMLWLYWMGKIFID
                     MLNGKRFVAIYILGGLTGAVFTIAAYYILPHLSQYVPAEIPMIGASASVLAIMTATAT
                     YFPNYIVHLLFIGPVRIKYLVLAMVLLDIILIPGINSGGHIAHLGGALFGFIWASQIK
                     KGTDISRWFMNIMDTIVTWTKPRKKITVSYSNPSSKSYSTKPTNAVTQQEIDAILDKI
                     AKNGYESLTAKEKEILFTMSKKR"
     gene            complement(2535..3275)
                     /locus_tag="P5243_03630"
     CDS             complement(2535..3275)
                     /locus_tag="P5243_03630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS18571.1"
                     /translation="MNYSPQGFTVLPPVVKNLLLLNAGVFIVGFIFQIMYQYDISDAL
                     GLHYIGSRAFKPIQFVSYMFLHANLEHIFFNMFALWMFGNVLENFWGPKRFFTFYMAT
                     GIGAGIVQMIVAYIRIQSLQNQVPAEVLDMIYSEGVDIIRQNKNFIDPVYAELNSIIN
                     VSTVGASGAVFGLLMAFGMLFPNSYIYLFFALPIKAKWFVLAYGIIELFSGIRNSPGD
                     NVAHFAHLGGMIFGYILIQYWRRKGTYL"
     gene            complement(3282..>3598)
                     /locus_tag="P5243_03635"
     CDS             complement(3282..>3598)
                     /locus_tag="P5243_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18572.1"
                     /translation="FGQNSFVVNGTPPDIEISTIENIIHAFISEYNERGESLQSNINE
                     TIARSLAQSSALYRNPVMQPDEMQTFIAQLFQCSMHSVSADGKRAVVMLEYDEIFKKF
                     NT"
BASE COUNT         1265 a          631 c          492 g         1210 t
ORIGIN      
        1 aacccataca atgcattcat aagattagta ggaaacgttt taatttttgt attaaacggt
       61 tgcaccatct cattgtagcg attacgttct acatttattc tattttctgt accttctaat
      121 tgagcttgaa gttctagaaa attttgattt gctttgaggt cggggtattt ctctaccaca
      181 accaacaaac gacttaaagc accactcatt tgattttgag cttcttgaaa ttttgcaatt
      241 gtttcgggtg ttaaattttc tgcatttact gttacgccgg ttgcttttgc acgagcttca
      301 attacgcttt gcaaagtact tttttcaaaa tctgcatacc cttttacagt ttttaccaaa
      361 tttggaatta aatcggcacg tcgttgatat gaactttcaa catttgccca ttgttttttt
      421 actgattctt gcattgtaac caaactattg tattgactaa ttaaatacaa tgcaagtatt
      481 acaatacctc caataataat ccatgttttt gtgtttttca tagctttaat tatttatgtt
      541 attttgaata ataaacgata ctgatatagg gaaatgatct gaaataggaa gtttttgttg
      601 tgtaaattct tgtactatta ctttttgtgg caaaagcaca taatctaatc gttttgataa
      661 taaacgccaa tttaatgttc ctcccaatcc atatgatgct tctaaaaaag catcttgcaa
      721 atctttttcg cttctaatag ctttataggt aaacgaatac ggtggtgcat taaaatctcc
      781 acatactatt gtttcatatg gattctctcg catagcttca agtaattcct gagtttgttt
      841 tcctcgtttt tgaattgcac gtttgagttt acctcctact tttcgtaatt tttgtgattc
      901 aaactcttga cccgtactgg tcatctctat ggtttcaata ttttcgggct gcaatttata
      961 cgattccaaa tgattattgt atatacgaac tgtatcatta tataatttaa tatcagccca
     1021 tagtgttgta tttcctgtct tttgatataa aataacaccc gtttttacaa taggaaattt
     1081 actaaaaata acatttccat aaaaatatct ccctttaatt tcatgaactc tgtggatttg
     1141 aatataccgt aagcctaatt gtttttttat actgtcaatc atttgatatt tttcatgcgt
     1201 atcatgatga aattcttgaa aacaaataat atccggcgat tcttgtttta aaaaatcgaa
     1261 cacttgagct tgaattccag cacgcccacg ccacccatta taattaaatg catttacatt
     1321 gtatgatata atttttacag tatcaccgct aacatcatgt ttgggagaac ctgcaaatga
     1381 aaaagtatca aaaaaggttg atatacttaa aacaaaacca ataacagaaa ctaaaacatt
     1441 caatttgtgt tttgtaaata accaaaatat gacaaaaaaa agatttacaa gtaaataaac
     1501 cggatatgct aatgcaacaa attcaaacat ccaaattaat tgtggattta tatatcgtgc
     1561 aatagcggta aatacaagca acaatgatgc aattaaattg acacaaaata aaataaatcg
     1621 aacaaacttc atgtattatc tttttttact catagtaaat aaaatttctt tttcttttgc
     1681 cgttaaactt tcataaccat ttttagcaat tttatctaat atagcatcta tttcttgttg
     1741 tgtaacagca tttgtaggct tagtagaata tgactttgaa gaaggatttg aataacttac
     1801 tgtaattttt tttcttggtt tagtccatgt tacaatagta tccataatat tcataaacca
     1861 tctagaaata tcggtaccct tttttatttg cgaagcccat ataaatccaa acaatgctcc
     1921 ccccaaatgt gctatatgcc cccctgaatt tattccggga ataagaataa tatcaagtaa
     1981 caccattgct aaaacaaggt atttaatacg aacaggacca ataaacaata aatgaactat
     2041 ataattagga aaatacgtgg ctgtagctgt cataattgca agtactgatg cagatgcgcc
     2101 tatcattgga atttcagcag gtacatattg tgataaatga ggtaaaatat aatacgctgc
     2161 tatagtaaat acagcacccg taagtccacc taatatatat attgcaacaa atcgttttcc
     2221 attaagcata tctataaata tttttcccat ccagtacagc cacaacatat tgaataaaat
     2281 atgcagaaaa tcaacatgag taaacatata cgtaaccaat gtccatggct taacaataag
     2341 ttttggaaca gaaatatatg tttgaatatt acttataata aaagtataga ttccggattc
     2401 ttgttgcccg gtaaaaatcc acgataatac ttgtaaaata ttaagaatca gaaaaactgc
     2461 tatgtttata aaaataatct ttataagatt atttccttcg cgaaattcgc gttgtatgtc
     2521 gtgccaaatt gtcattataa atatgttcct ttacgtctcc aatactgaat taatatatag
     2581 ccaaaaatca ttccacctaa atgagcaaaa tgagcaacat tatcgcccgg tgaattacga
     2641 attccactaa acaattctat aattccataa gccaaaacaa accatttagc cttaataggc
     2701 aatgcaaaaa ataagtatat ataggaatta gggaacaaca taccaaaagc cataagcaat
     2761 ccaaataccg cccccgaggc tccaacagtt gatacattaa taatagaatt caattcggca
     2821 taaacaggat caataaaatt tttattttgc ctgattatat caacaccttc agaataaatc
     2881 atatctaaaa cttcggccgg tacttgattt tgtaaagatt gaatacgaat atacgccact
     2941 atcatttgca ctattccggc tcctataccg gtagccatat aaaatgtaaa aaaccgtttg
     3001 ggtccccaaa aattttctag cacattacca aacatccaca aggcaaacat attgaaaaaa
     3061 atatgctcca aattggcatg taaaaacata taactcacaa attgaatggg tttaaaagct
     3121 cttgaaccaa tataatgaag accaagtgca tcggatatat catattgata cattatttga
     3181 aaaataaatc caacaataaa aacacctgca ttcaacaaca acaaattttt aactaccggt
     3241 ggcaatacag taaaaccttg tggagaataa ttcatacaat attaggtatt aaatttttta
     3301 aaaatttcat catattcaag cattactaca gcacgttttc cgtcggcaga aaccgagtgc
     3361 atagaacatt gaaacaattg tgcaataaat gtttgcattt catcgggctg cattacagga
     3421 tttctgtata atgccgatga ttgtgctaaa gaacgagcaa tagtttcatt gatatttgat
     3481 tgaagagatt cacctcgctc gttatattca gaaataaatg catgaataat attttctata
     3541 gtacttattt caatatccgg tggtgtacca tttaccacaa aagaattttg accaaata
//
LOCUS       DAOTOW010000150         3583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20650_length_3583_cov_2.971655, whole genome shotgun sequence.
ACCESSION   DAOTOW010000150 DAOTOW010000000
VERSION     DAOTOW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20650_length_3583_cov_2.971655"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /gene="mutL"
                     /locus_tag="P5243_03640"
     CDS             complement(<1..1366)
                     /gene="mutL"
                     /locus_tag="P5243_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRS18573.1"
                     /translation="MPDIIQLLPDSVANQIAAGEVVQRPASVVKELVENSIDAEATDI
                     IISIIDAGRTVIQITDNGKGMSDTDARMAFERHATSKIRTADDIFSIRSMGFRGEALA
                     SIAAVSHTELKTRTADTEIGTKIIIKASQVESQTPVSCPIGSTFTVKNLFYNIPARRK
                     FLKSNQTEFKYILSEILQIALAHPHIGFTIYKDDELYAKYPISNHKQRIVNIFGKRME
                     KSLLPIEGETSIVTISGYIGTPDIAKKNNYDQYFFVNNRFFRQKSYQNAIMRAYEQLI
                     PNREYPPFFVFFDIDPSQIDVNIHPTKTEIKFVEEYNICQLLEASVKHALGKSNIVPS
                     LDFIDTEDYSDMFAIPKDAPVKIPGIDVRTDYNPFKTDKMTHQAPQKQSVAGWEQLFE
                     GFETHSTVAQQIFSSAINTIHEEPASEPVTCMQIQKKYILTTGKTGLMIIDIKRAHSR
                     ILYEK"
     gene            complement(1378..1674)
                     /locus_tag="P5243_03645"
     CDS             complement(1378..1674)
                     /locus_tag="P5243_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18574.1"
                     /translation="MGIFSSIYSMRKPRGFSYSPRYYNPEKEKREERIQRILQEVDKE
                     QGKTTDSVQYIPHIRGQFKAKKDARSRIVHKQNVRIILIFIVLLYFAYLFITRI"
     gene            complement(1817..2449)
                     /locus_tag="P5243_03650"
     CDS             complement(1817..2449)
                     /locus_tag="P5243_03650"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRS18575.1"
                     /translation="MAVLVGKKAPDFDAKAVINGNQIIENFSLSQYKGKYVVLFFYPL
                     DFTFVCPTELLAFQHVLGEFEKRNVQVIGVSVDSEFSHWKWLQTPQNEGGIKGVTYPI
                     VSDISKTISENYDVLAGHYDVNEQGESTFVGAPVAYRGTFLIDKEGIVRHQVVNDLPL
                     GRSIEDALRMVDALQYFEQHGEVCPADWKPGKKALKATQEGISEFLSNNK"
     assembly_gap    2480..2489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2550..>3583)
                     /locus_tag="P5243_03655"
     CDS             complement(2550..>3583)
                     /locus_tag="P5243_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRS18576.1"
                     /translation="GFKPAEEAQTQWHAPRKFDKDTGIMVDVPDTALAPPKYQDVFGE
                     TLLELAQNDSRIVGVTPAMGSGCSMNILMQAMPDRAFDVGIAEQHAVTLSAGMAIDGL
                     IPFCNIYSSFMQRAYDQVIHDVALQRLPVIFCLDRGGLVGADGATHHGVFDIAFMRSI
                     PGIIVSSPLNEVDLRNLMFTAYKSGKPFVIRYPRGNGVLQDWKQPMQVYEIAKSEKIR
                     EGNTIAILSFGPLGNVAKQAADTVSQTLGKEVAVYNMRFAKPLDVDCIKKVCKSYSVI
                     ITIEDGVIQGGFGSAVLEQCSLYEYTGKVIRLGIPDSFIPHGTQEELYAYCKLDAAGI
                     EQLLISLLA"
BASE COUNT         1151 a          667 c          519 g         1236 t
ORIGIN      
        1 acttttcata taaaattcta ctatgagctc gcttaatatc tataatcatc aaccctgttt
       61 taccggtagt tagaatatat tttttttgta tttgcataca cgtaacaggc tcacttgcag
      121 gctcttcatg tatagtatta atagccgaac taaatatttg ttgtgcaacc gttgaatgtg
      181 tttcaaatcc ttcaaataat tgttcccacc ctgccaccga ttgtttttgc ggagcttgat
      241 gcgtcatttt gtctgtttta aatggattat aatcagttct gacgtcaatt cccggaattt
      301 taacaggagc atctttgggt attgcaaaca tatcagaata atcttcggta tcaataaaat
      361 cgagcgacgg aacaatatta cttttaccca atgcatgttt taccgatgct tctaataact
      421 gacaaatatt gtattcttca acaaatttta tttctgtttt tgttggatga atatttacat
      481 ctatttgaga aggatcaata tcaaaaaaca caaaaaaagg cggatattca cgatttggaa
      541 ttaattgttc atatgctcgc attattgcat tttgataact tttttgtcga aaaaaacgat
      601 tatttacaaa aaaatactga tcataattat tttttttagc tatatcaggt gttcctatat
      661 atcctgaaat agttactata gaagtttctc cttctatagg tagcaggctt ttttccatgc
      721 gttttccaaa tatattaact attcgttgtt tatgattgga aataggatac ttagcatata
      781 actcatcatc tttgtaaata gtaaatccta tatgaggatg agccaatgca atttgtaata
      841 tttctgataa tatatattta aattcagttt gattcgattt taaaaatttt cgacgtgcag
      901 gtatattata aaaaagattt tttacagtaa aagtactacc aattggacat gaaacaggtg
      961 tttgtgattc aacctgcgat gctttaataa taattttggt gccaatttct gtatctgccg
     1021 ttcgagtttt aagttcggta tgcgacactg cagcaattga tgccaatgct tcgcctcgaa
     1081 aacccataga ccgaatagaa aaaatatcat cggctgttct aattttagat gtagcatgtc
     1141 gttcaaaagc catacgagca tcggtatcac tcataccttt cccgttatcg gtaatttgaa
     1201 taacagttct tcctgcatct attattgaaa tgataatatc tgtagcttcc gcatcaattg
     1261 aattttccac taattctttt acaaccgatg ccggacgttg aacaacctca ccggcagcaa
     1321 tttgattagc aacagaatca ggtaataatt gaattatatc gggcattaac gtaacattta
     1381 aattcgagta ataaacaaat atgcaaaata cagtaaaaca ataaaaatta aaataatacg
     1441 aacattttgt ttatgtacaa ttcttgaacg tgcatccttt tttgctttaa attgtccacg
     1501 aatatgagga atgtattgca cggaatctgt tgttttccct tgttctttgt ctacttcttg
     1561 taaaatacgt tgtatgcgtt cttctctttt ttctttctca ggattgtaat atcggggcga
     1621 ataagaaaaa ccacgaggtt tacgcataga atatattgac gaaaaaattc ccataatcaa
     1681 ttttttacaa atatacggaa tctataattg ttatactttt ttaatcattc tttttattaa
     1741 aaaagcttta cccgaagaat ccgagtaaag cttttaagag aatacttact atatgaaaaa
     1801 aactaaaagg gagggactat ttattatttg ataaaaattc tgaaattcct tcttgagtag
     1861 cttttaatgc ttttttaccg ggtttccagt ctgcaggaca cacttcgcca tgttgttcaa
     1921 aatactgaag agcatctacc atacgcaaag catcttctat acttctacca agtggtaaat
     1981 catttacaac ctgatgacgc acaatacctt ctttatcaat caagaaagta cctctatatg
     2041 ctacaggtgc tccaacaaag gtagattctc cttgttcgtt tacatcataa tgccctgcaa
     2101 gcacatcata attttctgat attgtttttg atatatcaga tactatagga tatgttacac
     2161 cttttattcc accctcattt tgtggagtct gcaaccattt ccaatgtgaa aattctgaat
     2221 caaccgatac tcctattact tgtacatttc gtttttcaaa ttcacctaat acatgttgaa
     2281 aagctaaaag ttctgtagga catacaaacg taaaatctaa tggataaaag aataatacca
     2341 catattttcc tttgtattga ctcaatgaaa aattttcaat aatttgatta ccgtttataa
     2401 ctgcttttgc atcaaaatca ggtgcttttt ttcctactaa tactgccatt ttttttactt
     2461 tttttaatga tttaactttn nnnnnnnnnt acttacaaca ttaaaaaaaa aaattacaaa
     2521 aattagtatt ttttttattt agcaaacaat tatgctaaca acgaaatcag taattgctca
     2581 ataccggcag catctaattt acaatatgca tatagttctt cttgagttcc atgtggtata
     2641 aacgaatcgg gtattcctaa ccgtataact ttacctgtat attcgtataa agaacattgt
     2701 tcaagaactg cagaaccaaa tccaccttgt attacaccat cttcaatagt tataataaca
     2761 gaatacgatt tacaaacttt cttaatacaa tcaacatcta aaggttttgc aaatcgcata
     2821 ttatatacgg ctacttcttt tcctaatgtt tgcgacactg tatctgctgc ttgttttgct
     2881 acattaccca gtggcccaaa actaagaata gcaatagtat taccttcacg tattttttct
     2941 gattttgcaa tttcatatac ctgcattggt tgtttccaat cttgcaacac tccatttcca
     3001 cgaggatatc gaataacaaa aggttttcct gatttatatg ctgtaaacat taaattacgc
     3061 aaatcaactt cgtttaaagg tgatgatact ataataccgg gaatggaacg cataaatgca
     3121 atatcaaaca cgccatgatg agttgcgcca tcagctccaa ccaagccacc tctgtctaaa
     3181 caaaaaataa caggcaaacg ctgcaatgca acatcgtgaa taacttgatc gtatgcccgt
     3241 tgcataaaac tagaataaat attacaaaac ggtattaatc catctattgc catacccgcc
     3301 gacaatgtaa cagcatgttg ttcggcaata cctacatcaa aagctctatc gggcatagct
     3361 tgcataagta tattcataga acaaccggat cccatagctg gagttacacc tacaattctc
     3421 gaatcatttt gtgccaactc caacagagtt tcaccaaata catcttgata ctttggcggt
     3481 gccaatgctg tatccggcac atcaaccatt attccggtat ctttatcaaa ttttcgagga
     3541 gcatgccatt gtgtttgtgc ttcttcggca ggtttaaatc cct
//
LOCUS       DAOTOW010000151         3571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20728_length_3571_cov_3.175768, whole genome shotgun sequence.
ACCESSION   DAOTOW010000151 DAOTOW010000000
VERSION     DAOTOW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20728_length_3571_cov_3.175768"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="P5243_03660"
     CDS             complement(<1..467)
                     /locus_tag="P5243_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRS18577.1"
                     /translation="MIKKIINTIERNKISSVEVADAFNKTGVIDNIKAFNPGHFVVGK
                     VHYVCTYNESNWPLHEQIRDFDEDVILYVDSFNCQNRAVFGDIVSKYLYLYKRVKGII
                     VNGLIRDAHRIRKENYPLWCKGVTPLGCFNKEVQITKEIEQYIATQKKYFQGS"
     gene            complement(479..1672)
                     /locus_tag="P5243_03665"
     CDS             complement(479..1672)
                     /locus_tag="P5243_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRS18578.1"
                     /translation="MDKILVVGAGKYQISGIQKLQELGYFVIAVDGNQNCEGAAIADL
                     FYAIDIHNADAIIQTLLNENIQIISAMCFATEIALRTVAAINTYFTLPGLNNHDVSIA
                     TDKSLQRQILEKNNLPCPKYKELHIHSDMYNSIIDFGFPCIIKPTDNAGSRGVFLIHN
                     KYGLEEVIQECVINVHFDSKIIVEEFIPGIEFTVEAFSIDNEIIILGISEKKKPVNNF
                     TVSIELFYNSPLVETLRYRIEHIVINYLKACNFSNGITHTEIIYSFHDNNLYVVETTV
                     RSGGFYIFDKIVPFITDIDIVKHSILLQLGKSIMLPEMKRKPCILGFYYGNKGIVKKI
                     NILDEIKLLTNIEYDLFVKEGDSVNNLDSDGARFGYFISYGDNWENVYYQARLSEYLI
                     KFEIV"
     gene            complement(1665..2576)
                     /locus_tag="P5243_03670"
     CDS             complement(1665..2576)
                     /locus_tag="P5243_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18579.1"
                     /translation="MNILISAHTYQGYGLGNIYRSLFLYEYIANQTDILVHFESNNEI
                     AYSLASKYSIELKKTLLSEYDVVIYDSPYNFLYNIALPIEETFRVNALRSMTKCFIGL
                     DYCRFSENICDIAINLCNHNTEKISEFNGKIYEGIQYAILKPDVLEAKGKTIQKIATF
                     LVSIGGEDSHSYTLQIITKLRAYTQKFSVVVGPWHKDFEEIQKICNFNQIYHNVTNMG
                     ELISAHDIIICNGGTTLLESMYLEKQIIPVAQSEFEKHFISCCSSQFYTLDDIPILLN
                     QNNETINYSNIDTFGKKRILSIVLQHG"
     gene            complement(2573..3367)
                     /locus_tag="P5243_03675"
     CDS             complement(2573..3367)
                     /locus_tag="P5243_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS18580.1"
                     /translation="MNSIPIFDSLSHPTIDSHWILPKYYNSANIENLVSQMKQYNIIK
                     SFAVGMHGIGSYEQTKYYNLIAPFSDIFYPIAFLLIEPNDTQKSIQEKLKQIKKTGFK
                     GIKLHPRFSDFSIANPLLSYTIKTANELQLTVLFCTYFYHPSQNACSNSIENLQKLLL
                     QIQDCKIILLHAGTVRLLEMIEIARSFKNILLDLSFTVCKYEGSSIDLDIQFACNTFD
                     QRICIGSDFPEFSMQKLRERFNYFTQHISEEKAKNIGYKNLETYIQ"
BASE COUNT         1235 a          544 c          421 g         1371 t
ORIGIN      
        1 attgaccctt gaaaatattt tttttgagtt gctatatact gttctatttc ttttgttatc
       61 tgtacttctt tgttgaaaca acctaatggt gtaacacctt tacaccacaa aggataattc
      121 tcttttctta ttctgtgagc atctcgtatc aaaccattaa ctattatacc ttttactcgt
      181 ttatataaat ataaatattt tgaaactata tctccaaata cagctctatt ttgacaatta
      241 aaagaatcaa catataaaat tacatcttcg tcaaaatccc gaatttgttc atgcaatggc
      301 caattgcttt cattatacgt acacacataa tgcactttgc caaccacaaa atgccccggg
      361 ttaaatgctt taatattatc aataacccct gttttattaa aagcatcagc aacttctacg
      421 cttgaaattt tattgcgttc tattgtatta attatttttt taatcatcgt ttaatttttt
      481 atacaatttc aaatttaatt aaatattcac ttaatcgagc ttgataatat acattttccc
      541 aattgtcacc atacgagata aaataaccaa atcgagcgcc gtctgaatca agattattta
      601 cagaatcacc ctcttttaca aataaatcat attcaatatt tgtaagtaat ttgatttcat
      661 caagtatgtt tatttttttt acaattcctt tattgccata ataaaaccct aaaatacatg
      721 gttttctttt catttcaggt aacattattg atttgcctaa ctgtaaaagt attgaatgtt
      781 tcacaatatc aatatcagta ataaaaggta ctattttatc aaaaatataa aacccgccgc
      841 ttcttactgt tgtttctaca acatacaaat tattatcatg aaaactatat ataatttctg
      901 tatgagttat accgtttgag aaattacacg cttttaaata atttattaca atatgttcta
      961 ttctatagcg taatgtttct accaatggtg aattatagaa taattctatt gaaacagtaa
     1021 aattatttac aggtttcttt ttttctgaaa taccaagaat tattatttca ttatctatac
     1081 taaacgcttc aactgtaaat tcaatgccgg gtataaactc ttcaactatt atttttgaat
     1141 caaaatgtac atttattaca cactcttgta ttacttcttc aagtccatat ttattgtgaa
     1201 tcaaaaaaac acctctgctt ccggcattat cggttggttt aattatacac gggaatccaa
     1261 aatctataat tgaattatac atatctgagt gaatatgcaa ttctttatat tttggacatg
     1321 gcaaattatt tttttctaaa atttgtcgtt gaagtgactt gtctgtagct atagaaacat
     1381 catgattatt taatccgggc aatgtaaaat aagtatttat ggcagctacc gttcttaatg
     1441 ctatttcggt tgcaaaacac attgccgaaa ttatttgaat attttcattt agtaacgttt
     1501 gaattattgc atctgcatta tgaatatcta ttgcataaaa taagtcagca attgccgctc
     1561 cttcacaatt ttgatttcca tcaacagcaa ttacaaaata tccgagttcc tgtaattttt
     1621 gtattcctga aatctgatat ttccccgcac caactactaa aattttatcc atgttgtaaa
     1681 actattgaaa ggattctttt ttttccaaag gtatcaatat ttgaataatt tattgtttca
     1741 ttattctgat ttaataatat tggaatatca tctaaggtat aaaattgcga actgcaacaa
     1801 gaaataaaat gtttttcaaa ttctgattgt gctaccggaa taatttgttt ttctaaatac
     1861 atagattcca ataatgttgt tccaccgttg caaattataa tatcatgtgc tgatattaat
     1921 tcacccatat ttgtaacatt atgatatatt tgattaaagt tgcatatctt ctgaatttct
     1981 tcaaaatctt tgtgccatgg acctacaaca acagaaaatt tttgtgtata tgctcgtaat
     2041 tttgtaataa tttgtaatgt ataagaatgt gaatcttcac ctccaattga cactaaaaaa
     2101 gtagcaattt tttgaattgt ttttcctttt gcttctagca cgtctggttt taaaatagca
     2161 tactgtatac cttcatatat cttaccatta aattcagaaa ttttttctgt attatgattg
     2221 cacaaattaa ttgcaatatc acatatattt tctgaaaatc tgcaataatc taaaccaatg
     2281 aaacactttg tcatacttcg taaagcatta actctaaaag tttcttcaat tggtaaggca
     2341 atattataca aaaaattata tggtgaatca tatataacaa catcatattc acttaataaa
     2401 gttttcttca actcaataga atattttgaa gctaatgaat atgcaatttc attatttgat
     2461 tcaaagtgaa ctaaaatatc ggtttgattg gcaatatatt catataagaa caaacttctg
     2521 tatatattac ctaatccata gccttgatat gtatgagcag aaattaatat attcattgaa
     2581 tatacgtttc taaattttta taccctatat tttttgcttt ctcttcagaa atatgttgtg
     2641 taaaataatt aaatcgttca cgtaattttt gcatggaaaa ttcaggaaaa tcggaaccta
     2701 tacaaattct ttgatcaaat gtattacatg caaattgtat gtcaagatct attgaactcc
     2761 cctcatattt acatactgta aatgacaaat caagtaatat attcttgaaa gatctcgcta
     2821 tttctatcat ttcaagcaaa cgcacagtcc cggcatgcaa aagaataatc ttacaatctt
     2881 gtatttgcaa taacaatttt tgcaaatttt ctattgaatt actacacgca ttttgcgaag
     2941 gatgataaaa atatgtgcaa aaaagcactg taagttgtaa ttcatttgct gttttaatag
     3001 tatatgaaag aagtggattg gcaattgaaa aatctgaaaa tcttggatgc aattttattc
     3061 ctttaaatcc tgttttttta atttgtttta atttttcttg aatacttttt tgtgtgtcat
     3121 taggttctat aagaagaaaa gcaataggat aaaaaatatc tgaaaacggt gcaataagat
     3181 tgtaatactt agtttgttcg taagaaccaa taccatgcat tcctacagca aatgacttta
     3241 taatattata ttgtttcatt tgagaaacaa gattttcgat atttgctgaa ttgtagtact
     3301 taggaagaat ccaatgagaa tcaatagttg gatgcgataa cgagtcaaat attggaatag
     3361 aattcatact aattatttta tttttacaat acatttcaaa tatatactat tctattgaaa
     3421 aaacagtatt aaaaaaaaga gcaatcatat gattgctctt tttaaaaaat tatttcattt
     3481 taattttgaa atatcatatt tgaaaaatcg tactactata atactcccta ttaataggac
     3541 agtttagtta tcttttttag atataatttt c
//
LOCUS       DAOTOW010000152         3557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20823_length_3557_cov_3.543975, whole genome shotgun sequence.
ACCESSION   DAOTOW010000152 DAOTOW010000000
VERSION     DAOTOW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20823_length_3557_cov_3.543975"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1075
                     /locus_tag="P5243_03680"
     CDS             158..1075
                     /locus_tag="P5243_03680"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRS18581.1"
                     /translation="MNVAQTIQTTKKTLFSFEILPPLKGKGIETIYNTIDPLMEFNPQ
                     FINITSHREVYDANNNRVRRRPGTVAIAGAISHKYNIPVVPHILCGGFTRKETEYVLI
                     DLHFLGIDNVLALRGDALRSDEPYAPEPDGNTYARDLLRQISDMNKGKYIGDTEYYTS
                     TPTNFSCGVAGYPEKHYAAPDLDTDILHLKEKQDAGAQYIITQMFFDNTYYFNFVAKC
                     RAVGITIPIVPGIKPIGLKNQVDVLPKLFHIQLPKDLDSALRNCADDEQAKQIGTEWA
                     IMQAKELVAHKVPLLHLFTYSSAKQSVYS"
     gene            1092..3008
                     /locus_tag="P5243_03685"
     CDS             1092..3008
                     /locus_tag="P5243_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS18582.1"
                     /translation="MISLSNVAVRFGGFTLFDDISVMITEQDKIGLVGRNGAGKSTLL
                     KIIAQVDNPSEGSVVIPDGCKIGYLPQQMKVSNGKSVFDEVKTAFDDIQALQKKLVFL
                     QNQLSERTDYESDEYMKIIHEVTELTDYIHMHGANEIDGEIEQMLMGLGFVRTDFNRP
                     TSEFSGGWRMRIELVKLLLKKPDILLLDEPTNHLDIVSIEWLEKFLIQFKGAIILISH
                     DRRFLDAVTHKTFEISLHSLQSYPAPYSEYLILKEERYQQQLAAYENQQKMIADTEKF
                     IERFRYKATKSVQVQSRIKQLEKIERIEVEKDTHGAFKIAFPPAPRSGTVVVKIENVT
                     KKYGVKTILKDVVCTVERGQKIAFVGKNGEGKSTLIKCILQEIAYEGSITLGHNVQVG
                     YFAQNQDEILDENATVLETLDAIAVGEIRTKLRDILGAFFFSGEDVDKKVRVLSGGER
                     NRLAMAKLMLQPYNVLILDEPTNHLDIQSKAVLKKALMNYDGTVLLVSHDRDFLEGLT
                     THIYEVKETKVREVIGDLQEYLSQLHVETLLQSKQNEHKLQKQDINVNKREYEQRKEK
                     ERELRKIEKAIQKIEIQIQDIESQIHLLTTKFIEQTADITADDYKNHALLQDELERTM
                     IEWESLIQELEKKK"
     gene            complement(3012..>3557)
                     /locus_tag="P5243_03690"
     CDS             complement(3012..>3557)
                     /locus_tag="P5243_03690"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRS18583.1"
                     /translation="NTYSQKLVSKDSISLSDNTYLNHSPKTAAILSAACPGLGQIYNK
                     NYLKVPIIYAGFAGLGYGFSYYQKNYKEFKSVYDAYRMPYLEKGETPPTNIQLTVFGT
                     SGYYPENVREGRDYYRRYRDLTLIGIGTWYVLNVLEAYVYAHLYNFDTSDDLSIRCIP
                     YITPSQYSSSLSSGVTLYFRF"
BASE COUNT         1301 a          518 c          637 g         1101 t
ORIGIN      
        1 atactttttt caataattta aggagctatg agtatggaat tctctgtttt ttttatgtca
       61 atgctaatat acatgatgtg aaagtaaaac ttttgcatac aaacatataa gctcaatcat
      121 tttttatatt tttgtcaaat aaaaaaaaat taaaacaatg aatgttgctc aaacgataca
      181 aacaactaaa aaaaccttat tttcatttga gattctgcct cctcttaaag gtaaaggaat
      241 tgaaactata tacaatacta tagacccttt gatggaattt aatcctcaat ttattaatat
      301 tacatcacat cgcgaggtgt atgatgccaa caataataga gtgcgtcgta gaccgggtac
      361 ggttgctatt gcaggagcta tttctcataa atacaatata ccggtggttc ctcatatttt
      421 atgcggtggt tttacacgta aagaaaccga atatgtactt atagatttac attttttggg
      481 aattgataat gtgcttgctc taaggggcga tgctttgcga agtgatgaac cgtatgcgcc
      541 agagcctgat ggtaatacct atgcacggga tttgcttcgt caaataagtg atatgaataa
      601 aggaaaatat attggtgata cagaatacta tacatctact ccaacaaatt tttcgtgtgg
      661 agtggcagga tatcctgaaa agcactatgc tgcccctgat ttagatactg atattttgca
      721 tttaaaagaa aaacaagatg ccggggccca gtatatcatt actcaaatgt tttttgataa
      781 tacttattat tttaattttg ttgccaaatg cagagctgtt ggtattacta tacctattgt
      841 tcccggaatt aaaccaatag gcctcaaaaa ccaagttgat gtattaccaa aattatttca
      901 tatacaatta ccaaaagatt tagattctgc tttgcgtaac tgtgctgatg atgaacaagc
      961 aaagcaaata ggaacagaat gggcaattat gcaagcaaaa gaattggttg ctcacaaagt
     1021 gccattgttg catttgttta catatagcag tgcaaaacaa tctgtatata gctaaagctg
     1081 tgttttaaat tatgatttct ctttcaaatg tagctgttcg gttcggaggg tttaccttat
     1141 tcgatgatat ttctgtaatg attaccgaac aagataaaat tggtttggta ggtagaaatg
     1201 gtgcaggaaa atctacatta cttaaaatta ttgctcaagt agataatcca agtgaaggtt
     1261 cggtggtaat tcccgatggc tgtaagattg gatatttgcc acagcaaatg aaagtaagta
     1321 atggtaagtc tgtatttgat gaagtaaaaa cagcttttga tgatatacaa gcattacaga
     1381 aaaaacttgt gtttttacaa aatcaattgt cagaacgaac agattatgag tctgatgaat
     1441 atatgaaaat tattcatgag gttactgaac ttactgacta tattcatatg catggagcaa
     1501 atgaaattga tggtgagatt gaacaaatgt tgatgggatt aggatttgtg cgaacagatt
     1561 ttaatagacc tacctccgaa tttagtggtg gttggcgtat gcgtattgaa ttagtaaaat
     1621 tgcttcttaa aaaacctgat attcttttac ttgatgagcc tacaaatcat cttgatattg
     1681 tttcaataga gtggctcgaa aaatttttga tacaatttaa aggtgcaatt atactaattt
     1741 cgcacgacag aagattttta gatgctgtaa cacacaaaac atttgaaata agtcttcatt
     1801 cattacaatc atatcctgct ccttatagtg aatatcttat acttaaagaa gaacggtatc
     1861 aacaacaatt ggctgcatat gaaaatcaac aaaaaatgat tgccgatacc gaaaagttta
     1921 ttgaacgttt tcgatacaaa gcaacaaaat cagttcaagt gcaatcgcgt ataaaacaac
     1981 ttgaaaagat tgaacgaatt gaagtagaaa aggatacaca tggtgctttt aaaatcgctt
     2041 ttccgcctgc tccccgatca ggaacagtag ttgtaaaaat tgaaaatgta actaaaaaat
     2101 atggtgttaa aacaatactt aaagatgtag tttgtacagt agaacgtggg caaaaaattg
     2161 cgtttgtcgg taaaaatggc gaaggaaaat ctacattaat taagtgtatt ttacaagaaa
     2221 tagcatatga agggtctata acattggggc acaatgtgca agtaggatac tttgctcaaa
     2281 atcaagatga aatacttgat gaaaatgcca cagtactcga aacacttgat gcaattgccg
     2341 taggtgaaat acgaacaaaa ttgcgagata ttttaggtgc atttttcttt agtggtgaag
     2401 atgttgataa aaaagttcga gtactttcag gtggcgaacg caatagatta gcaatggcta
     2461 aattaatgtt gcagccttat aatgtattaa ttctcgatga gcccacaaat catcttgata
     2521 tacaatcgaa agctgttctt aaaaaagcat tgatgaatta cgatggtacg gtacttctag
     2581 tatcgcatga tcgcgatttt ttagagggac ttactaccca tatttatgaa gtaaaagaaa
     2641 caaaagtacg cgaagtaatc ggagatttac aagaatattt atcgcaatta catgttgaaa
     2701 cattattgca gtcaaagcaa aatgaacaca aactacaaaa acaggatata aacgttaata
     2761 agcgagagta tgaacaacga aaagaaaaag aacgtgaatt gcgaaaaatt gaaaaagcaa
     2821 tacaaaaaat agagatacaa attcaagata tagaatcaca aatacattta cttacaacta
     2881 aatttataga gcaaacagca gatataactg ccgatgatta taaaaatcac gctctattac
     2941 aagatgaatt agaacgaaca atgatagaat gggagagttt aattcaagaa ttagagaaaa
     3001 aaaaatagcg attaaaacct gaaatataat gtaacacccg aacttaatga agaagaatat
     3061 tgagaagggg ttatatatgg aatacagcga atacttaaat catcacttgt atcaaaatta
     3121 tataaatgag catatacata ggcttctaac acgtttaata cataccacgt tcctattcct
     3181 ataagtgtta aatctcgata tcttctataa tagtctcgtc cttctcttac attttcaggg
     3241 tagtatcctg aagtgccaaa taccgtaagt tgtatatttg ttggtggtgt ttcacctttt
     3301 tcaagatatg gcattctgta ggcatcataa acagacttaa attctttgta atttttttga
     3361 taatatgaaa aaccgtatcc caaacctgca aaaccggcat aaataatagg tacttttaaa
     3421 taatttttgt tataaatttg tcctaatcca ggacaagcag cagaaagtat agcagcagtc
     3481 tttggagaat ggtttaaata cgtattatca gataaagaaa tagaatcttt tgaaactaat
     3541 ttttgtgaat atgtatt
//
LOCUS       DAOTOW010000153         3540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20926_length_3540_cov_3.484648, whole genome shotgun sequence.
ACCESSION   DAOTOW010000153 DAOTOW010000000
VERSION     DAOTOW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20926_length_3540_cov_3.484648"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="P5243_03695"
     CDS             <1..456
                     /locus_tag="P5243_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18584.1"
                     /translation="TQSISLQSGWNLFSYSVRTADSTITTLFAGKDVQEIKNADGFWF
                     KNNTAELQSLTKLTPGKGYLVKMNTAATVQITGTPIVSPSYALLPAGWNLIGYPSAGT
                     TPFTVLPISNFLNATNSQIVKNFDGFWEPNGTTNSITNFEAGKGYYLKK"
     gene            464..1129
                     /locus_tag="P5243_03700"
     CDS             464..1129
                     /locus_tag="P5243_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRS18585.1"
                     /translation="MSKPDSIIFDLDGTLWDAVETYAMGFNAYFKDHGIEKYFTKQDL
                     QGFMGLEQHAYLQAVLPEFAIHEQNTIYEKVIEYQYTMVKLHGGTLYTGVAEGIPLLA
                     ETYRLFIVSNCPADMIYHFLKWSKLEKYFTHTISHGQNHKSKYENIQDLRTFFALRNP
                     IYVGDTYSDYVQSKKAFVPFVFMQYGFGTCITCHKSFSHFQDFVNFYTANSTATYPTV
                     SCM"
     gene            1216..2745
                     /locus_tag="P5243_03705"
     CDS             1216..2745
                     /locus_tag="P5243_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRS18586.1"
                     /translation="MKKICVILLSVTLVLAAHAQSAKKLYKLYHAREFVKCVEKCNDV
                     IAKYPNFFEPYYIQAISYFEMAQLPEKYKDFTKDPLMDCIKTLSILRAKDPDGTIFEE
                     HSDTLQMIYNYADAVAKSLRNTNKDKAILMYQRLMRAYNIHANALELATIYAKVGDYE
                     RCMSQVSRLYEKYKPEISSSHEDYEALTKGAILLAENWMFRDLFWIIETYRDKYQNNY
                     AISQGFKKALKMSIDTARYDEDKTFFQDFSKRGMELYPNDADIQKHIEKRWVELIDNT
                     AQKYRETAGAPRTWRDSVLLRDTYKYMEMAKQIMPGSTVFEEKQKKLNAEFHIIPFSF
                     EQEFFRKMSLQIVNQWRTEGCLCDTGRLVKVYPVPELQWNTILEDLAQSHAKEMFCYN
                     FTDNINQAGETPWDRIKKTDLKGITYENERGLNYIQALKIGEILGYGYSFDGVYSEEE
                     MQKVIEAVVKKWIETRLSQNCMKLMTPDFTHMGMAMYGDKWVLFAATIHDIVIKSNKK
                     K"
     gene            2742..3341
                     /locus_tag="P5243_03710"
     CDS             2742..3341
                     /locus_tag="P5243_03710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRS18587.1"
                     /translation="MSLLPYMRKGILEAGCDEAGRGCLAGPVFAAAVIFPKDYTNSLL
                     QDSKKLTETQRTKLRRQIETDALTWAIGVVSHIEIDSINILQASFLAMHRALDTLVCK
                     PQHILVDGNRFKPYNAIDYTCVVKGDGTYMSIAAASILAKTYRDEHMEQIHNEYPYYN
                     WLQNKGYPTLAHRKAVIEYGLSPYHRKSFNTSIQLQLEF"
     gene            complement(3343..>3540)
                     /locus_tag="P5243_03715"
     CDS             complement(3343..>3540)
                     /locus_tag="P5243_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRS18588.1"
                     /translation="RKTLVSTGDRSAKIRTYNFPQGRVTDHRINLTLYNLPAIMDGDI
                     QEIIDKLIVEENAERLKASEN"
BASE COUNT         1234 a          546 c          635 g         1125 t
ORIGIN      
        1 acacaaagta tttcattgca aagcggttgg aacttgtttt cttatagcgt tcgtacagcc
       61 gattctacta ttacaactct ctttgccgga aaagatgtac aagaaattaa aaatgctgat
      121 ggtttttggt tcaaaaataa tactgctgaa ttacaatcat tgactaaact tactcccggt
      181 aaaggatatt tggttaaaat gaatactgct gctactgtgc aaattaccgg taccccaata
      241 gtttctccaa gttatgcatt attacctgca ggttggaacc ttattgggta tccttcggca
      301 ggaactacac cttttaccgt attaccgatt tcaaattttt taaatgcaac taattcacaa
      361 attgtcaaaa attttgacgg attttgggaa ccaaatggaa ctactaattc tattacaaat
      421 tttgaagcag gtaaaggata ttacttaaaa aaataatgtt tctatgagta aacctgattc
      481 aattattttt gatttagacg gtacattgtg ggatgctgtt gaaacttatg ctatggggtt
      541 taatgcatat tttaaagacc atggtataga aaaatatttt acaaaacaag atttgcaagg
      601 atttatgggg ttagaacaac atgcatactt gcaggcagta ttacccgaat ttgcaattca
      661 tgaacaaaat actatatatg aaaaagtaat tgaataccaa tataccatgg ttaaactaca
      721 tggtgggact ttgtatacag gtgttgcaga aggaattcca ttgttggcag aaacatatag
      781 attatttatt gtaagtaatt gtcctgcaga tatgatttat cattttctca agtggagtaa
      841 gcttgaaaag tattttaccc atactatttc tcatggccaa aatcacaaat caaaatacga
      901 aaacatacaa gatttacgaa cgttttttgc acttcgaaat ccaatatatg tgggcgacac
      961 atattcagac tatgttcaaa gcaaaaaagc ttttgttccc tttgttttta tgcaatatgg
     1021 atttggtact tgtataacat gtcataaatc attttctcat tttcaagatt ttgtaaattt
     1081 ttatacagca aacagtacag caacatatcc tactgttagt tgcatgtaat gttactgtaa
     1141 aactataatt tgattcatag tatttttcac aaaacaatta ctatttttgt ggggtagtaa
     1201 cgtttgtaaa aaagtatgaa aaaaatatgt gtcatattat taagtgtaac tcttgtatta
     1261 gcagcgcatg cacaatctgc aaaaaaatta tataaactgt atcatgcacg tgaatttgtg
     1321 aaatgtgttg aaaaatgcaa cgatgtaatt gctaaatatc caaatttttt tgagccatat
     1381 tatatacaag ctatttccta ttttgaaatg gcacaattac ccgaaaaata taaagatttc
     1441 acaaaagatc ctcttatgga ttgtataaaa acactgagta ttttaagagc aaaagacccc
     1501 gacggtacta tattcgaaga acattccgat actttacaaa tgatatataa ttacgccgat
     1561 gctgttgcta aatcattgcg taataccaat aaagataaag ctattcttat gtatcaacgt
     1621 cttatgcgtg catataatat tcacgcaaat gcgttggaat tagcaacaat atatgcaaaa
     1681 gtaggtgatt atgagcgttg tatgtcacaa gtttcacgat tgtatgaaaa gtataaaccc
     1741 gaaatctcat cgtcacacga agattacgaa gcccttacta aaggagcaat tctacttgcc
     1801 gaaaattgga tgtttcgcga tttattttgg attatagaaa catatcgtga taaatatcaa
     1861 aataattatg ctataagtca agggtttaaa aaagccttaa aaatgagtat agatacagca
     1921 cgatacgatg aagataaaac attttttcag gatttttcga aacgtggaat ggaattgtac
     1981 cctaatgatg ccgatataca aaaacatatt gaaaaacgat gggttgaact cattgataat
     2041 acagctcaaa aatatagaga aactgcgggt gctcctcgta catggcgtga cagtgttttg
     2101 cttcgcgaca catacaaata tatggaaatg gctaaacaaa ttatgcctgg cagtactgta
     2161 tttgaggaaa aacaaaaaaa gttaaatgct gaatttcata ttattccctt tagttttgaa
     2221 caagaatttt ttcgtaagat gtcgttgcaa attgtaaatc agtggcgtac cgaaggctgt
     2281 ttatgtgata caggaaggtt ggtaaaggta tatcctgttc cggaattgca atggaataca
     2341 atcctcgaag atttagcgca aagtcatgct aaagaaatgt tttgttacaa ttttaccgat
     2401 aacataaatc aagccggcga aaccccttgg gataggatta aaaaaacaga tttaaaaggc
     2461 ataacgtatg aaaatgaacg tggacttaat tacatacaag ctcttaaaat aggtgaaata
     2521 ttaggctacg gatattcttt tgatggtgtg tactccgaag aagaaatgca aaaagtaata
     2581 gaagctgttg taaaaaaatg gattgaaacc cgattgagtc aaaattgtat gaaattaatg
     2641 acgcctgatt ttactcatat gggtatggct atgtatggag ataaatgggt actctttgcc
     2701 gccactattc atgatattgt aattaaatca aacaaaaaga aatgagttta ttgccctata
     2761 tgcgtaaagg tatactagag gccggatgcg atgaggcagg cagagggtgt ttggcaggac
     2821 ccgtatttgc agctgctgta atttttccca aagattatac taattcattg ttacaagatt
     2881 ctaaaaagtt gacagaaact caaagaacaa aattgcgtag acaaatagaa acagatgctt
     2941 taacatgggc tattggtgta gtgtcgcaca tagaaataga ttctataaat attttgcaag
     3001 catcgttttt ggcaatgcac agagcattag atacattagt ctgtaagcct caacatattc
     3061 ttgttgacgg taacagattt aaaccttata atgctataga ttatacatgt gtagtaaaag
     3121 gagatggtac atatatgtca attgctgcag cgtcaatcct tgctaagact tatcgtgatg
     3181 aacatatgga acaaattcat aatgaatacc cttattataa ttggttgcaa aacaaaggct
     3241 atccaacgtt ggcacaccga aaggctgtaa tagaatatgg gttaagccca tatcatagaa
     3301 aaagttttaa tacttctatt caactacagt tagaatttta gattaatttt cgcttgcttt
     3361 taagcgttct gcattttctt ctactattaa cttgtcaatt atttcctgaa tatcaccatc
     3421 cataattgcc ggcagattgt ataaagtaag gtttattcgg tgatcggtta cacgtccttg
     3481 cggaaaatta tacgtgcgta tttttgccga acggtcacct gtcgaaacta atgttttacg
//
LOCUS       DAOTOW010000154         3536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20947_length_3536_cov_3.924160, whole genome shotgun sequence.
ACCESSION   DAOTOW010000154 DAOTOW010000000
VERSION     DAOTOW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20947_length_3536_cov_3.924160"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="P5243_03720"
     CDS             <1..414
                     /locus_tag="P5243_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS18589.1"
                     /translation="SSKFENQRDIEYYKLEQCTYSKHQIIPVTDYFFTENSQKNKDVK
                     SRKAYIPNTFTPGGNDENDVFFFNTNGVDVFTVWIYNSWGGLVYKQEALTISWTGNDN
                     LGMKCPSGVYYYVVQSSNRDKRHETSGFIQLFRQD"
     gene            429..2024
                     /locus_tag="P5243_03725"
     CDS             429..2024
                     /locus_tag="P5243_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS18590.1"
                     /translation="MYIIRKLTYDHVIWIILLFTVSFFTWGNFFITVSSIAMLTVWIL
                     QKNFSYKFQILWQRKTPLFLLSIYLLIFIGALVYVPNPKAIKDIWDNAPMLVYALVLG
                     SSKQLTRKQFHVVMLLYIISIVINTVYCFVYFLYVADNYTDVRELSVFMSYIRLALYT
                     LMAIAGTGYYLFYNTRVRIHTKEKVFLWLALFWLIFFVIILSSITGFVGLALLFLFFT
                     ISQIQKQKHIVLKLLPLSIIIIGIWFVTDTVTKELHYFTNPSTVEIQKLDSVTLLGNP
                     YAPFTKTGRLENGNWVDLYVCEKEIVENWPLYSSKPIFGNDEKGHILYYTLKRYMTSK
                     NLRKDASGLKQLSQEDIRNIHAGCTNYRFDKSFNLSHRIYEVIWEFHNYKIGRNPAGH
                     SVTQRIEFLKCAYEVFKKHMWFGTGVVHITEKLHEQYAKQPIKMPQKFWKKPHNQFML
                     VAVQYGIVGFTVFISSILGIIYFSRKNLNILSISWLSICLISFFNEDMLDGINGLVFF
                     SFFAIFMLCIQPVKIEYFNKKQV"
     gene            2112..2199
                     /locus_tag="P5243_03730"
     tRNA            2112..2199
                     /locus_tag="P5243_03730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2146..2148,aa:Ser,seq:tga)
     gene            2371..>3536
                     /locus_tag="P5243_03735"
     CDS             2371..>3536
                     /locus_tag="P5243_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18591.1"
                     /translation="MVCFQSIAQIPSIVFTQNKNQWDDRVLYRSQLYQGFLYAEKQGF
                     TYYFTDGTHKRHSHSVAHGEVEHEYDEQNLKQQLSDFVNGTVSVDTSTIQPLSSCNSH
                     EKKEFKAHAYAVQFINMNKKASIVAHKPISGIENYYIGNNPQKWAQGVQSFETITYKG
                     VYSNIDLSLYNNNGSLKYDFIVKPGGKPSQIQLRYTGVPKMEIDINGNLIIYTSVNEV
                     FEKKPIAYQIIENDTIFVECAYKLEYFTLSYTVGIYNPKYTLYIDPELVFSTYTGSIA
                     DNWGFTATYDSENNVYAGGIVDAFGYPTNIGALQETFGGGTWDVGIIKYDPTGTQRLY
                     ATYLGGTSAEMPHSLIANSNNELLILGTTGSHDFPMQNAYDPTFNGGKNILYDNA"
BASE COUNT         1227 a          484 c          596 g         1229 t
ORIGIN      
        1 agcagtaaat ttgaaaatca acgagatata gaatattata aattagaaca atgtacgtat
       61 tcaaaacatc agattattcc tgttaccgat tattttttta ctgaaaattc ccaaaaaaat
      121 aaagatgtta aaagtcgtaa agcgtatatt cctaacacat ttactcccgg aggcaatgat
      181 gaaaatgatg tgtttttctt taatacaaat ggtgtagatg tttttacagt atggatttac
      241 aatagttggg gtggactggt ttataaacaa gaggctttaa ctatttcatg gacaggaaac
      301 gataatttgg gaatgaagtg tccatcagga gtttattatt atgttgtgca atctagtaat
      361 agagataaac gacatgaaac ttcagggttt atccaattat ttcgtcagga ttaagtattt
      421 ctatttatat gtatattata cgtaaactca catatgacca tgtaatatgg attatactat
      481 tgtttacagt tagctttttt acgtggggta atttttttat aacagtttca agtattgcta
      541 tgctaactgt ttggatatta caaaagaatt tttcatataa atttcaaata ctttggcaac
      601 gtaaaacccc tttattttta ttaagtattt atcttttgat ttttattgga gcacttgttt
      661 atgtaccaaa tcctaaagca ataaaagata tttgggacaa tgctccaatg ttagtttatg
      721 ctttggtatt gggtagtagt aagcaattaa cgcgcaaaca atttcatgtt gtaatgctgc
      781 tatacattat ttctattgta ataaatactg tatattgttt tgtgtacttt ttgtatgttg
      841 ccgataatta tacagatgta cgcgaattaa gtgtatttat gtcatatatt agattggcat
      901 tgtatacact tatggcaatt gcaggcacag ggtattattt attttataat actcgggtac
      961 gaatacatac caaagaaaag gttttcctat ggcttgcttt attttggctt atcttttttg
     1021 ttattatttt aagttctata acaggatttg ttgggttagc attattgttt ttatttttta
     1081 caatttctca aattcaaaaa caaaaacata tagtgctcaa attattgcca cttagcatta
     1141 taattattgg aatttggttt gtaacagata ctgtaaccaa agaattacat tattttacaa
     1201 atccaagcac tgttgaaatt caaaaattag attcagtaac cctgttagga aacccatatg
     1261 ctccatttac aaaaaccgga cgattagaaa atggtaattg ggttgattta tatgtgtgtg
     1321 aaaaagaaat tgttgagaat tggcctctat atagttcaaa gccaatattt ggaaatgatg
     1381 aaaaaggtca tattctatat tatacactta agcggtacat gacatcaaaa aatcttcgca
     1441 aggacgcatc gggccttaaa caactaagtc aagaagatat tagaaatatt catgccggct
     1501 gtacaaatta ccgatttgat aaatcgttta atttatcgca tagaatttat gaagtaatat
     1561 gggaattcca taattataaa attgggagaa atccggcggg ccattcggta acacagcgta
     1621 tagaattttt aaaatgtgca tatgaagtat ttaaaaaaca tatgtggttt ggaacaggag
     1681 tggtacatat taccgaaaaa ttacatgaac agtatgcaaa acaaccaatt aaaatgcctc
     1741 aaaaattttg gaaaaaacct cataatcaat ttatgcttgt agctgttcag tatggaattg
     1801 ttggttttac tgtttttatt agtagtattt tgggaattat ttatttctct cgaaaaaatc
     1861 ttaacatact ttcaattagt tggttgagta tttgtttaat atcttttttc aatgaagata
     1921 tgcttgatgg aataaatggg ttggttttct tctcattttt tgctatattt atgctttgta
     1981 ttcaacctgt aaaaattgaa tatttcaaca aaaagcaggt gtaagctttt gaaaatcaaa
     2041 tcaaattaat ctttttataa aatatatgca aatacaattt gtattattca aaaaaattgt
     2101 atttttgcag cggagaaatg gcagagtggt tgaacgcggc ggtcttgaaa accgttttac
     2161 ggcaacgtaa cgggggttcg aatccctctt tctccgccaa aaaaatataa ggccctaact
     2221 tgaataaagt tagggctttt aatttttttt gactgcaaac aaattgactc aaaatttgat
     2281 agctatagta attttttgta attttatcac aaaatcagac caatttaaat gatgaatata
     2341 caaaaaatcg ggttgaatat ttggtttatt ttggtatgct ttcaaagtat agctcagatt
     2401 ccttctatcg tttttacaca aaataagaat caatgggatg atcgtgtatt gtatcgatcc
     2461 caattatatc aaggattttt atatgcagaa aaacaaggat ttacatatta ttttactgat
     2521 ggcacacata aacgacattc gcatagtgta gcgcacggtg aggtagagca tgagtatgat
     2581 gaacaaaatt taaagcaaca attgtctgat tttgttaatg gaacagtatc tgttgatacg
     2641 tcaaccattc aaccgttatc atcttgtaat tctcacgaaa aaaaggaatt taaagcacat
     2701 gcatatgccg ttcaattcat aaatatgaac aaaaaagctt caatagttgc ccataagcca
     2761 atttcaggaa ttgaaaatta ttatatagga aacaatcccc aaaaatgggc acaaggtgtt
     2821 caaagttttg aaactataac ttacaaaggt gtatattcca atatagatct ttctttatat
     2881 aacaacaatg gaagtctcaa atatgacttt attgtaaaac cgggaggaaa accttctcaa
     2941 attcaattac gatatacagg tgttcctaaa atggaaatag atataaacgg taatcttatt
     3001 atttacactt cagtaaatga ggtgtttgag aaaaaaccta ttgcatatca aataatagaa
     3061 aatgatacta tttttgttga atgtgcctat aaacttgaat attttacatt aagctatact
     3121 gttggtatat ataatcctaa atatacatta tatattgacc cggaattagt ttttagtacc
     3181 tataccggtt ctattgccga taactggggt tttactgcaa cttacgacag cgaaaataat
     3241 gtgtatgcag gaggtattgt agatgcattt ggttatccca caaatattgg agcattgcaa
     3301 gaaacatttg gtggaggcac atgggatgta ggtattatta aatatgaccc taccggaaca
     3361 caacgtttat acgctacata tttaggaggt acaagtgcag aaatgcctca tagtttaatt
     3421 gcaaatagca acaatgaact attaattctt gggacaacag gttcacatga ttttcctatg
     3481 caaaacgcgt atgatcctac gtttaatggt ggaaaaaata ttttatatga caatgc
//
LOCUS       DAOTOW010000155         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_20998_length_3529_cov_3.616292, whole genome shotgun sequence.
ACCESSION   DAOTOW010000155 DAOTOW010000000
VERSION     DAOTOW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20998_length_3529_cov_3.616292"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2720)
                     /locus_tag="P5243_03740"
     CDS             complement(<1..2720)
                     /locus_tag="P5243_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18592.1"
                     /translation="MQIQKYIFIVFILCCNALQAQFYNGTQTSFGKNRVQYHEPFWRY
                     YRYQEFDVYYDKAGKQLAEFVAKHAIESQKEVKKIIDFRYTRRIIFVVYNSLENLKQS
                     NIGLSSSDDQYNIGGTTQIIDNKVILYFNGDLNNLQQQIRYGIALLMIREFLYGAENY
                     RQIISNSALHSYPLWFIEGMAEYISGSWNSILEEAVIIGFENKKFKNIVHTKPDDALI
                     IGHAMWKYIAESYGNQTITNILFVTKVTEDIQEGFNYVLKKNLKEIISETETYYKKNR
                     NIEETPIVFKQTIHIPKKAKKYPITQMQISPDGSKILYVTNNKGKATIYISNLENKKH
                     KKIYTTGTILEQITDYSYPVITWHPRSESCTFFVEKKGSIYMYTYIINTKELITREFH
                     YFEKVLECAYAKNGTEIVFSGVKNGQTDLYIYNTQTYANTQITNDRADDRFPVFIQND
                     SKILFSSNRNSIQTNTKQDSLRTTFDIFIASRTKNQNIQRIQDSYFDNELKPIEFMNG
                     SYIYINDKNGIQNLYRTTPDSTVHFIDTTIHYRYFHTTYPITNSKHNILDFSYSPVTS
                     DLILYTKQKKYNQISTVSLETLLNSHFENIQTINSKYIHDTSLQVLKQSKTAPIQNTP
                     QIDENIDFESYTFEFETNPSNYIVDSIFNSDSTYSRIRIPRLYETNFYINQIVNQVDF
                     GFINTSYQAFTGNAFYYMPGINAFFKFGVIDLFEDYRLTGGVRFTGNLQTNEFLFSIE
                     NLKKRWDKQLLFHKQSLLTYSADGVSGTSILYHKLQDYNTIFLLKYPINQIQAIVLSP
                     HVRYNKDVIVSTDITSLNKPSQTNIWAGFSCKYIYDNTRPKALNILNGTRAKLFTEIY
                     TDIPEIQKFLFVTGFDIRNYIKIYKNFIFAQRMAYSYSYGKSPLLYYLGSV"
     gene            complement(2783..>3529)
                     /locus_tag="P5243_03745"
     CDS             complement(2783..>3529)
                     /locus_tag="P5243_03745"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS18593.1"
                     /translation="TMFAENFTDYFIFYKPKDIVSGDFYWFQTFENIKVLAVADCTGH
                     GVPGGFMSMLGITLLNEIVIKNQNINAAHILQKLRKEIIAALHQTGNLGEQRDGMDIA
                     LTIIDTNFMKLRFAGAFNSIYCIRSKELSEIKADSKVELNDYILYELKADRMPISHYT
                     VMEPFSQKEFDLLPNDLIILFSDGFQDQIGGSNNKKYLSVRFKNFLLTHSSHRCKDIK
                     IHLEKELQTWKNVGVNQEQIDDITIIGIRI"
BASE COUNT         1106 a          448 c          530 g         1445 t
ORIGIN      
        1 acacttccca gataatatag taaaggtgac tttccgtatg aatagctata agccattcgt
       61 tgagcaaaaa taaaattttt ataaattttt atatagtttc ttatgtcaaa gcctgttaca
      121 aataaaaatt tttgtatttc gggaatatcg gtatatattt cggtaaataa ttttgcccgg
      181 gttccattta aaatattcaa agcttttggt ctggtgttat catatatgta tttacatgaa
      241 aatccggccc atatatttgt ttgtgatggt ttatttaacg atgtaatatc tgtagaaact
      301 attacatctt tattgtatcg cacatgcgga cttaaaacta tagcttgaat ttggtttata
      361 ggatatttta gtaaaaatat tgtattgtaa tcttgaagtt tatgatataa aatactagtt
      421 cctgaaacac catctgctga ataggtgagt agtgattgtt tatgaaatag caattgttta
      481 tcccagcgtt tttttaaatt ttctatggag aataaaaatt cgtttgtttg taagtttccg
      541 gtaaatctta ctcctccggt taatctataa tcttcaaata aatcaatgac tccaaatttg
      601 aaaaacgcat taattcctgg catataataa aaggcatttc cggtaaatgc ctgatatgat
      661 gtgtttataa atccaaaatc aacctgattt acaatttgat ttatgtaaaa gttagtttcg
      721 tataaacgtg gaattctaat tcgcgagtaa gttgagtctg aattgaaaat actgtcaaca
      781 atgtaattac ttggatttgt ttcaaattca aatgtatatg attcaaaatc aatattttca
      841 tctatttgtg gtgtgttttg tatgggggct gtttttgatt gttttaaaac ttgtaatgag
      901 gtatcatgta tatattttga attgatagtt tgaatatttt cgaaatgtga attaagtaaa
      961 gtttctaaag atacagttga tatttgattg tatttttttt gttttgtata tagtattaag
     1021 tctgaagtta caggagaata tgaaaagtct aaaatattat gttttgaatt agttatagga
     1081 tatgtagtat gaaaatatct gtaatgaata gttgtatcaa taaaatgtac agtactgtct
     1141 ggtgttgtgc gatataaatt ttgaattcca tttttatcat ttatatatat atacgaacca
     1201 ttcataaatt caattggttt tagttcatta tcaaaatatg aatcttgaat acgttgaata
     1261 ttttggtttt ttgttcgtga agctataaaa atatcaaatg ttgttcttaa agagtcttgt
     1321 ttcgtatttg tttgtattga atttctatta gatgaaaata aaatttttga gtcattttgt
     1381 ataaatacgg ggaatctatc atcggctcta tcgttagtaa tttgtgtgtt tgcgtatgtt
     1441 tgtgtgttat atatatataa atctgtttgt ccatttttga cacctgaaaa aactatttct
     1501 gtaccatttt ttgcatatgc acattccaat actttttcaa aatagtgaaa ttcacgtgtt
     1561 ataagttctt ttgtgttgat gatatatgtg tacatataaa ttgagccttt tttttctaca
     1621 aaaaatgtac atgattcact acgcggatgc catgtaataa caggatagga ataatcggta
     1681 atttgttcta gaatagttcc tgtggtatat atttttttgt gttttttatt ttctaaatta
     1741 gaaatataaa tagtcgcctt tcctttatta ttggttacat acagtatttt gctgccatcg
     1801 ggtgatattt gcatttgtgt aataggatat ttttttgctt ttttgggtat atgtatagtt
     1861 tgtttaaata ctattggtgt ttcttctata tttctatttt ttttgtaata tgtttctgtt
     1921 tcggatataa tttcctttaa attttttttt aatacataat taaatccttc ttggatatct
     1981 tcggtaactt ttgttacaaa aagtatgttt gtaatagttt gatttccata ggattccgca
     2041 atatatttcc acatggcatg acctataata agggcatcat cgggttttgt atgtactata
     2101 tttttaaatt ttttattttc aaatcctatg ataacagctt cttccaaaat gctattccat
     2161 gaaccggaaa tatattctgc cattccttca ataaaccata acgggtatga atgaagtgct
     2221 gaatttgata ttatttgtct atagttttct gctccatata aaaactctct tatcattaat
     2281 aaagctatgc catatcgtat ttgttgttgt aaattattta aatcaccatt gaagtataaa
     2341 attactttgt tatcaattat ttgagttgta ccgcctatat tatattggtc atctgatgaa
     2401 cttaaaccaa tattactttg tttaagattt tctagtgaat tgtatactac aaaaataatt
     2461 cttcgggtat atcgaaaatc aataattttt ttaacttctt tttgtgattc aatggcgtgt
     2521 tttgcaacaa attctgctaa ttgttttcct gctttatcgt aataaacatc aaattcttga
     2581 tatcgataat atcgccaaaa tggttcatga tattgtactc gatttttgcc aaagctagtt
     2641 tgtgttccat tataaaactg agcttgtaat gcgttacaac aaagtataaa tactataaat
     2701 atatattttt gtatttgcat ttgtaaataa tagggttaca aatgtagaaa aaagatgttt
     2761 atatatttgc aggaataata atttatattc gtataccaat tattgtaata tcgtcaattt
     2821 gttcttgatt aactccaaca tttttccatg tttgaagttc tttttctagg tgtatcttta
     2881 tgtctttgca gcgatgtgaa ctatgtgtga gtaaaaagtt tttaaatcgt acgcttaaat
     2941 attttttgtt gttgcttcct ccaatttgat cttgaaatcc gtctgaaaat aaaataatta
     3001 aatcgttagg aagtaaatca aattcttttt gagaaaatgg ttccattaca gtgtaatgag
     3061 aaattggcat acgatcagct tttaattcat ataaaatata atcatttaat tcaacttttg
     3121 aatctgcttt tatttcagat aattctttag atcgtataca atagattgaa ttaaaggcgc
     3181 cggcaaagcg taatttcata aaatttgtat ctataatggt aagggctata tccataccat
     3241 cacgttgttc ccctaaattc cctgtttgat ggagagctgc aattatttct tttcgtaatt
     3301 tttgtagaat atgggctgca tttatatttt gattctttat tactatttca tttaacaaag
     3361 taattcccaa catgctcata aaaccacctg gaacaccatg tccggtacag tctgctaccg
     3421 ctaaaacttt tatattttca aatgtttgaa accaataaaa atcgccactc acaatgtctt
     3481 ttggtttata gaatataaaa taatcggtaa aattttctgc aaacattgt
//
LOCUS       DAOTOW010000156         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21053_length_3522_cov_3.012691, whole genome shotgun sequence.
ACCESSION   DAOTOW010000156 DAOTOW010000000
VERSION     DAOTOW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21053_length_3522_cov_3.012691"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="P5243_03750"
     CDS             complement(<1..867)
                     /locus_tag="P5243_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS18594.1"
                     /translation="MISNKRVLVTGGAGFIGSNLIEALLAANNTVVCLDNFATGYKSN
                     IQRFETNPFFSLIEGDICDENVCKNAVQSIEIVFHHAALGSVPRSIADPVTSTKVNIL
                     GFVNMLTAAKDAGVSRFIYASSSSTYGNADYSPKVETKKGTLLSPYAVTKQTNDLFAQ
                     VFSELYNIQTIGLCYFNVFGPYQDPQGAYAAVIPKFIACLLNKQAPTIYGNGSQSRDF
                     TYVDNVVHANLLAAEATLTNNHEHINIACSESIDLTTLTMYMIRSIAHYMPEVNTITP
                     IYTNVRKGDIQHS"
     gene            complement(869..2401)
                     /gene="rny"
                     /locus_tag="P5243_03755"
     CDS             complement(869..2401)
                     /gene="rny"
                     /locus_tag="P5243_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRS18595.1"
                     /translation="MEILIYSTLSFIIGATLTLVLQKTLLQAKAKSIISEAEKEAEVI
                     KKDKMLQAKEKFLQLKAEHEKVINERNNKILQGENRIKQKEDSLRLKLEEIQRKEKEI
                     ETKKEQLTNQIQINERKTEDLERAHKQQIEQLEAISGLSAQEAKEQLIETLKAEAKTE
                     AMSLINEIMDEAKMTANKEAKKLVVQTIQRTATEHVVENSVSVFHIESDEVKGRIIGR
                     EGRNIRALEAATGIEIVVDDTPEAIILSGFDPIRREIARLALHQLVTDGRIHPARIEE
                     VVEKTRKQVEEEIIETGKRVCIDLGIHGLHPELIRHIGKMKYRSSYGQNLLQHSKETA
                     KLCGIMASELGLNAKAAKRAGLLHDIGKVAEDDGELPHALAGMKLAERFKEKPEICNA
                     IGSHHEEVEMETLIAPIVQVCDAISGARPGARREIVESYIKRLKDLETIALSYPGVLK
                     TYAIQAGRELRVIVGAEKVTDSDTEVLSMNIAKKIQEEMTYPGQVKITVIRETRAVNY
                     AK"
     gene            complement(2646..2939)
                     /locus_tag="P5243_03760"
     CDS             complement(2646..2939)
                     /locus_tag="P5243_03760"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRS18596.1"
                     /translation="MSEKLSIKLRILERNYPLLIEWKEEEKLREAAKRINDLVIRFKQ
                     HYTDKDNQDLLAMVSLQFASKLLDLEGNQNTQNIETRIEQMCEELDEFIKINT"
     gene            complement(2936..3229)
                     /locus_tag="P5243_03765"
     CDS             complement(2936..3229)
                     /locus_tag="P5243_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18597.1"
                     /translation="MSQNIELLVVRLQEKIKILTSMYTILQQQNAELQKNNKELHTTI
                     EQQTNTIKELERNYKNLQIAKAVSQTGGDTSEAKRKIEEIVREIDTCIALLNK"
BASE COUNT          938 a          579 c          556 g         1449 t
ORIGIN      
        1 tgaatgttgt atatctccct tacgaacatt ggtatatatt ggggttatag tgtttacttc
       61 gggcatatag tgagctatgc ttcgaatcat gtacattgta agagttgtta aatcaataga
      121 ttcactacat gctatattta tatgttcatg gttgtttgtt aaggtagctt ctgctgccag
      181 taaatttgca tgtacaacat tgtctacata ggtaaaatca cgtgattgcg aaccgtttcc
      241 atatatagtt ggtgcttgtt tgttgagtaa acaggcaata aatttaggaa ttaccgcagc
      301 atatgcacct tgaggatctt ggtatggacc aaatacattg aaataacata aaccaattgt
      361 ttgaatgttg tataattctg aaaacacttg agcaaataag tcattggttt gttttgttac
      421 tgcgtatggc gataaaagag tacctttttt ggtttctact tttggtgaat agtcggcatt
      481 accataggta gatgaactgc ttgcatatat aaatcgtgaa actccggcat cttttgctgc
      541 agttagcata tttacaaatc ccaagatatt aactttagtt gacgttaccg ggtcagcaat
      601 agaacgaggc accgaaccta atgctgcatg atgaaatact atttctattg attgtacagc
      661 atttttacaa acgttttcat cacaaatatc accttctata agtgaaaaaa aaggattggt
      721 ttcaaatctt tgtatgtttg atttatatcc ggttgcaaaa ttgtctaaac aaacaacagt
      781 gttattggca gccaaaagag cttcaattaa attagaacca ataaatccgg cacctccggt
      841 taccaataca cgtttgtttg aaatcatatt attttgcgta atttacagct ctggtttcac
      901 gaattacagt tattttaact tgcccaggat aggtcatttc ttcttgaatt tttttagcaa
      961 tattcataga aagaacttcg gtatcgctat cagttacttt ttcggcacct acaattacac
     1021 gtagttcacg tccggcttga attgcatatg ttttgagtac acccggatat gaaagagcta
     1081 tagtttctaa gtctttgagt cgttttatat atgattctac aatttcgcgg cgagcccccg
     1141 gacgagcacc ggaaatagca tcgcatacct gtacaatagg agctataagt gtttccattt
     1201 ctacttcttc atgatgtgat cctatggcat tgcatatttc gggtttttct ttgaatcgtt
     1261 ctgccaattt catacctgcc aatgcgtgtg gtaattcacc atcgtcttcg gctactttac
     1321 ctatatcatg gagtaaacct gctcgttttg cagcttttgc attcaaacct agttctgagg
     1381 ccataatgcc acaaagtttt gcagtttctt ttgaatgttg taataaattt tgtccgtacg
     1441 atgaacgata tttcatttta cctatatgac gaataagttc ggggtgtagg ccatgtatac
     1501 ctaaatctat acatactcgt ttacctgttt ctattatttc ttcttcaact tgttttcgag
     1561 ttttttccac tacttcttct attcgtgcag ggtgaattcg tccatcggtt actaattggt
     1621 gtaatgctaa tcgtgctatt tcgcgacgaa tagggtcaaa tcctgaaaga ataatagctt
     1681 cgggagtgtc atctactaca atttcaatac ctgtagctgc ttccaaagct ctaatgtttc
     1741 taccttcgcg tccaattata cgtcctttta cttcatcgct ttctatatga aataccgata
     1801 ccgaattttc tactacatgt tctgttgctg ttcgttgaat tgtttgaact accaattttt
     1861 tcgcttcttt gttggcagtc attttagctt cgtccattat ttcatttata agcgacatag
     1921 cttctgtttt tgcttcggct tttaaagttt ctatcaactg ttcttttgct tcttgagctg
     1981 aaagtcccga aatagcttct aattgttcaa tttgttgttt gtgagctcgt tctaaatctt
     2041 cagtttttcg ttcgtttatt tgaatttgat ttgtaagttg ttcttttttt gtttctattt
     2101 ctttttcttt tcgttgaatt tcttctaact ttaatcgcaa actgtcttct ttttgtttaa
     2161 ttctgttttc accttgtagt attttgttgt tacgttcatt tattactttt tcatgttcag
     2221 ctttcagttg taaaaacttt tcttttgctt gcagcatttt gtcttttttt ataacttctg
     2281 cttctttttc tgcttcgctt attattgact tagcttttgc ctgtagtaat gttttttgca
     2341 gcactaatgt aagtgtagct cctattataa aggaaagagt tgaataaatt agtatttcca
     2401 tacgtgttgt ttagtttaag ttagttatat atatataaaa agctcgtatc aaaatcttaa
     2461 attgtagaaa accccaaaat acagggtttg gagtgtaaac tatatctaac ttccactgtt
     2521 ttttgattca aaaaaacgcc gaagcgttga ggcctgttat caatagtcgt aacgttctcc
     2581 ggtttagtgt aaacgttgag ttttcacatt atttaagaaa ttaatacgag ctatattttt
     2641 tatttctatg tatttatttt tataaattca tcaagttctt cacacatttg ttctatacgg
     2701 gtctcaatat tttgtgtatt ctgattccct tctaaatcga gtaatttact tgcaaattgt
     2761 aaacttacca ttgctaataa atcttgattg tctttgtcgg tatagtgttg cttaaaacga
     2821 attactaaat cgtttattcg ttttgctgct tcccgaagtt tttcttcttc tttccattct
     2881 ataagtaatg gatagttacg ttcaagaatt cttagcttta ttgataattt ttcactcatt
     2941 tatttaataa tgcaatacat gtatctattt ctcgtacaat ttcctcaatt tttcgtttgg
     3001 cttcacttgt gtcgcctccc gtttgggata ctgcttttgc tatttgtaaa tttttataat
     3061 tccgttctaa ttcttttatt gtatttgttt gttgttctat tgttgtatgt aattctttgt
     3121 tatttttttg taattcagca ttttgttgtt gtaatatagt atacattgat gttaaaattt
     3181 ttattttttc ttgtaatcta acaacaagta gttctatatt ttgactcatc ttatctatag
     3241 tatattttaa ttgttacaaa aatagtatgt taaaattatt tttacaatgc tctttctact
     3301 tttttttaat ttattcaata ataattttaa ttaatttttt taattgattg aaatttagtt
     3361 gtttatttct ttttttgtga tttaatataa aaattttgca tacgtattta tgtttttacg
     3421 gatatatttt tttatgtgac taaaatcaca cataaacgat tgataataaa aaatcactgt
     3481 tgtccgataa aaaattaaga gaaggaagat gttagtcttc ct
//
LOCUS       DAOTOW010000157         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21104_length_3516_cov_3.752962, whole genome shotgun sequence.
ACCESSION   DAOTOW010000157 DAOTOW010000000
VERSION     DAOTOW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21104_length_3516_cov_3.752962"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="P5243_03770"
     CDS             complement(<1..535)
                     /locus_tag="P5243_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18598.1"
                     /translation="MDEDNDTIFNNNDYLAIFERCEHMLDSKIETYFDVYEFTELLDN
                     YIEQGKLNKAYTLVQIAQKQHPNNTELEFRKARIHIERNDFETAVDILKKLLRIEEDN
                     SDYHICIGVAYTYKHDFTAAVKHFTIGITLSPENEEDNLYNIGVTFLNVDNYKLAYDY
                     LWQAYTKYPNNLLALYDL"
     gene            complement(538..2091)
                     /locus_tag="P5243_03775"
     CDS             complement(538..2091)
                     /locus_tag="P5243_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRS18599.1"
                     /translation="MFTDSQILEIKNYGLQVETVEKQIERFKIGFPTINITATATIGN
                     GIIQCDEKEALKYAKLFDKKKKRKKIVKFVPASGAATRMFKMLYTMLETYDGSEESYK
                     KLFSNKGLHTPAGFIENIEKFAFFPQLSQILASDGYSIEELLQKKDYTTILTYILTEK
                     GLNYGNLPKGLLLFHSYGNIYRTALEEHLVEGALYAKNHNKDVYIHFTVSPEFIQGFK
                     SKVAETKYSYQETYSVKYYTDYSIQKPSTDTIAVNVDNTPFINNDGSLLFRPAGHGAL
                     IENLNDLDFDIIFIKNIDNVVPDSQKDKTILYKKVLGGILIHYQSAIFKFYDKISKSK
                     HLSDKRFRLVTKFISEELQFKFPEGFETWPRNDCKEYILGILNRPIRVCGMVKNEGEP
                     GGGPFFVLEKDGSQTLQIIEKAQIDTKNKQQLDFLNASTHFNPVDLVCGIKDANGKKY
                     NLTEFVDAESGIITTKSKDGKELKALELPGLWNGAMANWNTIFVEVPIETFNPVKEVT
                     DLLRKEHIQ"
     gene            complement(2210..>2282)
                     /gene="hisH"
                     /locus_tag="P5243_03780"
     CDS             complement(2210..>2282)
                     /gene="hisH"
                     /locus_tag="P5243_03780"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841208.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRS18600.1"
                     /translation="AFQFHPEKSGEIGAKILENFIKL"
     assembly_gap    2283..2292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2293..2812)
                     /gene="hisH"
                     /locus_tag="P5243_03785"
     CDS             complement(<2293..2812)
                     /gene="hisH"
                     /locus_tag="P5243_03785"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRS18601.1"
                     /translation="MKIAIVKYNAGNIQSVSYALNRLGIEPIITNDPDKLYKADKIIF
                     PGVGEASSAMAYLRAHGLDKILTTLTQPFLGICFGMQLMCKHSEEGNVDCLGIFDVQV
                     KRFPSSKEFKIPHMGWNTIQNHKGTLLTNIPEQSFVYFVHSYYAELNEHTTTTCNYIL
                     PYSAGIEKDNFFA"
     gene            complement(2817..>3516)
                     /gene="trpA"
                     /locus_tag="P5243_03790"
     CDS             complement(2817..>3516)
                     /gene="trpA"
                     /locus_tag="P5243_03790"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRS18602.1"
                     /translation="ELNSTVRIIKLLQEHGVDMIEIGMPFSDPLADGNVIQHSSTVAL
                     RNGMTVSLLFSQIENIRKTVTIPLLLMGYINPVLQYGVEKFCESCKHVGIDGVILPDL
                     PLHEYIKDYKHIFDAYGIQNILLISPQTEEQRIRMIDEHTNAFIYMVSSSSTTGTKSQ
                     FSDEQIQYFKRIQSMQLQNPLLVGFGISNTETFKQVCLYTHGAIVGSACIKALEKEGT
                     LEQNISEFVASIKG"
BASE COUNT         1042 a          539 c          551 g         1374 t
ORIGIN      
        1 ctaaatcgta gagtgccaat aaattattag ggtattttgt atatgcttgc cataaataat
       61 catatgctaa tttataatta tcaacgttaa gaaatgtaac acctatatta tataaattat
      121 cttcttcgtt ttcagggctt aatgttatgc ctattgtaaa atgttttacg gcagctgtaa
      181 agtcatgttt atatgtataa gctaccccta tacaaatatg ataatcggaa ttgtcttctt
      241 ctattcgcaa taatttttta agaatgtcaa cagcagtttc aaagtcattt cgttctatat
      301 gaatacgagc ttttctaaat tctaattctg tattattcgg gtgttgtttt tgagctattt
      361 gaacaagggt atatgcttta tttaatttgc cttgttctat ataattatca agtaactcgg
      421 taaattcgta tacatcaaaa tatgtttcaa tttttgaatc aagcatatgt tcgcatcgtt
      481 caaaaattgc taaatagtcg ttattgttaa aaatagtatc gttgtcttcg tccatagcta
      541 ttgtatatgt tctttgcgaa gtaggtcggt cacttctttt accggattga aggtttctat
      601 aggaacttct acaaaaatcg tgttccaatt tgccattgct ccgttccata atcccggtaa
      661 ttctaatgct tttaattctt taccgtcttt actctttgtg gttattatac ctgattctgc
      721 atcaacaaat tcagttaaat tatatttttt accatttgca tctttaattc cgcatactaa
      781 atctacagga ttaaagtgtg ttgatgcatt taaaaaatct aattgttgtt tgttttttgt
      841 gtcaatttga gctttttcaa ttatttggag agtttgtgaa ccatcttttt ctaaaacaaa
      901 aaatggacct ccacccggtt cgccttcatt tttaaccatt ccgcatactc gtataggtct
      961 gtttaaaatt cctaatatat attctttgca atcatttcga ggccatgtct caaatccttc
     1021 aggaaattta aattgtaatt cttccgatat aaattttgta actagtcgga atcgtttatc
     1081 agataaatgt ttgctttttg aaattttatc gtaaaatttg aatatggcag actgataatg
     1141 aattaaaatt ccgcctaata cttttttata caatattgtt ttatcttttt gagaatcagg
     1201 aacaacattg tctatatttt ttataaagat tatatcaaaa tctaaatcat ttaaattttc
     1261 aattagagca ccatgtccgg caggtctaaa caacaaactt ccgtcattgt ttataaatgg
     1321 ggtattgtct acatttactg caatggtatc ggtacttggt ttttgaatac tatagtcggt
     1381 atagtatttt accgaatatg tttcctgata tgaatattta gtttcagcta cttttgattt
     1441 aaatccttga ataaattcag gtgaaaccgt aaagtgtatg tatacatctt tattatgatt
     1501 tttggcatac aaagctcctt ctaccagatg ttcttctaaa gctgtacggt atatattccc
     1561 ataggaatga aacaataaca gacctttggg taaattacca taatttaatc ctttttcagt
     1621 taaaatatat gtaagtatgg ttgtataatc ttttttttgt aaaagttctt ctatagaata
     1681 gccgtcggat gcaagaattt gactcagttg ggggaaaaat gcaaattttt cgatattttc
     1741 aataaaaccg gcgggggtat gtagtccttt gtttgagaat agttttttgt atgattcttc
     1801 actgccgtca tacgtttcga gcatggtata cagcatttta aacatacgtg ttgctgctcc
     1861 cgaagcaggt acaaatttta caattttttt acgtttcttt tttttatcaa aaagtttggc
     1921 atatttcaat gcttcttttt catcacattg tattatgccg ttgccaatag ttgcggttgc
     1981 tgtaatattt atagttggaa atccgatttt aaagcgttct atttgttttt ctacagtttc
     2041 aacttgaagc ccgtagtttt tgatttctaa aatttgtgaa tcggtaaaca tatactctta
     2101 gtttttataa ttttatcgaa aatacataaa atggctgtaa aagcaataca ttgggtatgt
     2161 atttttttgt ggagttgtta acaagttatg ttaatcaatc actgaggttt taaagtttta
     2221 tgaaattttc taaaattttt gcacctattt ctccactttt ttcggggtga aattgaaaag
     2281 cannnnnnnn nnaagcaaaa aagttatctt tttcaattcc tgcactataa gggagtatat
     2341 aattacaggt agtagttgta tgttcattta attctgcata gtagctgtgt acaaaatata
     2401 caaaactctg ttcaggtata tttgtaagca gggttccttt atgattctgt attgtgttcc
     2461 atcccatatg aggaatttta aattcttttg atgagggaaa tcgtttgacc tgaacatcaa
     2521 aaattcctaa acaatcaaca ttgccttctt cggagtgttt acacataagt tgcataccaa
     2581 agcaaattcc taaaaatggt tgagtaaggg tagtaagtat tttgtcaagt ccatgagcac
     2641 gcaagtatgc catagcacta cttgcttcac caactcccgg aaaaataatt ttgtctgctt
     2701 tatagagttt atcggggtca ttggttatta taggttctat acctaatctg ttaagtgcat
     2761 aactcaccga ttgtatattt cctgcattat attttactat agcaatcttc atatgcttat
     2821 cctttaattg atgctacaaa ttctgaaatg ttttgttcta atgttccttc tttttctaac
     2881 gcctttatac aagcactacc tacaattgct ccatgtgtgt ataaacatac ttgtttaaat
     2941 gtttctgtat ttgaaatacc aaatccaact aatagtggat tttgtaattg catagattga
     3001 attcgtttaa aatactgaat ttgttcgtca gaaaattgtg atttagtacc tgtagttgac
     3061 gatgatgaaa ccatatatat aaatgcgttg gtgtgttcat ctatcattcg aattctttgt
     3121 tcttctgttt ggggtgatat taataatata ttttgaatac catatgcatc aaatatgtgt
     3181 ttgtagtctt ttatatattc gtgtaatggt aaatcaggga gtataacacc atctattcca
     3241 acgtgtttgc atgattcgca aaatttttct acgccatatt gcaataccgg atttatgtaa
     3301 cccataagta ataaaggtat agttactgtt tttctaatgt tttctatttg agaaaataat
     3361 aatgatactg tcattccatt gcgtaatgcc actgtgctgc tatgttgaat tacattgcca
     3421 tcggcaagtg ggtcagaaaa tggcattcct atttcaatca tatctactcc atgttcttgg
     3481 agtaatttaa taatgcgaac tgtagaattg agttca
//
LOCUS       DAOTOW010000158         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21106_length_3516_cov_3.663970, whole genome shotgun sequence.
ACCESSION   DAOTOW010000158 DAOTOW010000000
VERSION     DAOTOW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21106_length_3516_cov_3.663970"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..897
                     /locus_tag="P5243_03795"
     CDS             10..897
                     /locus_tag="P5243_03795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS18603.1"
                     /translation="MLFSIIIPTYNRAHILPHTIASVLAQTYTNWECIIVDDGSTDNT
                     KELIASYTDPRIRYIYQTNAERSAARNNGIRHAQGEYICFLDSDDEYLPEHLEVMERE
                     IKNNNSPIIGMYFTNAVFTQSGTIWKPDFSKLRAEHELNYMLNNPIIPARVCIHSHIL
                     QHVQFREDIVIVEDQVLWATIAMHYPVYHIQEYTVRYHLHEDNSVNWKKNCFTPRLQG
                     LQKFFEQEDARKHIPKSKRNEMLSDCYFGIARFYLHNNQYWNMCKNLILSIIQKPSHP
                     QTKAKVHMMVFPNKHIQNR"
     gene            989..1756
                     /locus_tag="P5243_03800"
     CDS             989..1756
                     /locus_tag="P5243_03800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS18604.1"
                     /translation="MKQFFLKIYNYPLLTPEQTNANQKRARDCEWDAIEPYIQKGAFL
                     DVGCGAGYSMYKAQQIGCEVFGIDPDPQGHGVGRRNSDFIVPIGTITQGFSEQLPYND
                     NTFNTVYTSHVLEHVTSIEQSLQEIKRVCKNDGIIIIGVPTATMAWINWFTQLMFTTH
                     HKIVNTLFSPFINTGKTFWWEIFIPRSHSFPHKKTVISDIIHYSTKKWKSRIEKQFEI
                     IHIIKPCLYPYPEYRQLFSLRKNKYCSSSVFFICKQT"
     gene            1753..2787
                     /locus_tag="P5243_03805"
     CDS             1753..2787
                     /locus_tag="P5243_03805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HRS18605.1"
                     /translation="MIQKIKHTITSWFWVISYSKIEKCIHYRKEKPFEHAKLPDNETI
                     KKHFHRDNSTHYHEEFIYEFKNVIVESECGYILENFRRIHHTTCSTKNRLPSVSNYIS
                     KKMRRKIIYESACIFDAHIGNNYFHFFSDVITKLWILKKYNLDRLPLFIGQDVYYSTI
                     FQFLISNSTISQYNWHVLDNQHLYTITKQLFVCKPMSYDITLWLQTLSLFKLPVLQNR
                     RIFVYRNKNTSRYLYNTETISKILKQYGFESIDTSNLPIREQIRYFAESNIIIGVHGA
                     GLTNMMFACHNQPTIIEIIPENRIACQFYWMSTMFQFNYFPILGSSIIQNEFSVDTNE
                     LEQTILKIIT"
     gene            complement(2851..>3516)
                     /locus_tag="P5243_03810"
     CDS             complement(2851..>3516)
                     /locus_tag="P5243_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18606.1"
                     /translation="IAVLGGYGEAVSDIKFPEWFVKYQNFYAVGEQFPHNGRVFGVRN
                     VVYGAGMIVQRKAWEYIVSKGFTFFTLGRTGKSLSSGEDSEMCLAFQIAGFDIWYNNS
                     LRFKHYIEPKRLTITYLQRLQKGMSSSGYVTQFYRRFLFGYTPKITRYFWFKEFVYSI
                     KDLLKGFFVNSTKLGLRRNFHFSIYLLRERGKYNKNVAKVIEICENLSKNVIEDMSNQ
                     VIK"
BASE COUNT         1280 a          629 c          468 g         1139 t
ORIGIN      
        1 atatctacca tgcttttctc catcataatc cccacataca accgtgctca cattctcccc
       61 cacactattg caagcgtgct ggcccaaacc tataccaatt gggagtgcat tatagttgac
      121 gatggcagta ccgacaatac caaagaactc atagcatcat acaccgaccc acgtattcgc
      181 tacatatacc aaacaaacgc cgaacgcagt gctgctcgca acaatggtat ccgccatgcg
      241 caaggggaat atatttgttt tttggatagt gatgatgaat acctgccgga gcatttggaa
      301 gtgatggaac gagagattaa gaataataat tctcctatta tagggatgta tttcacgaat
      361 gcagttttta ctcaatctgg caccatatgg aaacctgatt tttcaaaatt acgagcagaa
      421 catgaattaa attatatgct caacaatcca atcataccgg cacgagtatg tattcattcg
      481 catattttac aacatgttca atttcgtgaa gatattgtaa ttgtagaaga tcaggttttg
      541 tgggcaacta tagctatgca ttaccccgta tatcatattc aggaatatac tgtccgctat
      601 catctgcatg aagataattc ggtaaattgg aaaaaaaatt gttttactcc ccgattacaa
      661 ggattacaga aattttttga acaagaagat gctcgcaaac atatacccaa atcaaaacgc
      721 aacgaaatgt tgagtgattg ctatttcggc attgcacgtt tttatttgca taacaatcaa
      781 tattggaata tgtgtaaaaa cttaattctt tctattattc aaaagccatc tcatccacaa
      841 acaaaagcaa aggtacatat gatggtattt ccgaacaaac atattcaaaa caggtaggat
      901 tatttcacca cagatagcac agatttacac agaacataat ctgtgtaaat ctgtgctatc
      961 tgtggtaaaa atttcttact tttgaatcat gaaacaattt tttctaaaaa tatataacta
     1021 cccattacta actcccgagc aaacaaatgc caatcaaaaa cgtgcacgcg attgtgaatg
     1081 ggatgctatt gaaccatata ttcaaaaagg cgctttttta gatgttggtt gtggtgccgg
     1141 atatagtatg tataaagccc aacaaatagg ctgtgaagta tttggtatag accccgaccc
     1201 tcaggggcat ggcgttggaa gaagaaattc tgattttatt gttccaatag gaactattac
     1261 acaaggattt tcggaacaat taccatacaa cgacaataca tttaataccg tttacacatc
     1321 gcatgtgctt gaacatgtta caagtattga gcaatcgtta caagaaatta agcgtgtatg
     1381 taaaaatgat ggaattataa taataggcgt gccaacagca accatggcat ggattaattg
     1441 gtttacacaa cttatgttta ctacgcatca taaaattgta aatacattat tttcaccttt
     1501 tattaatacc ggaaaaacat tttggtggga aatttttata ccacgttcgc attcattccc
     1561 acacaaaaaa acggtaattt cagatattat acattactca acaaaaaaat ggaaatctcg
     1621 tattgaaaaa caatttgaaa ttatacacat tataaaacct tgtctatatc cctatcccga
     1681 atatcggcaa ttatttagcc ttcgaaaaaa caaatattgt tcaagttctg ttttttttat
     1741 ttgtaaacaa acatgattca aaaaataaaa catacaataa catcatggtt ttgggtaatt
     1801 tcttattcaa aaatagaaaa atgtattcat tacagaaaag aaaaaccatt tgaacacgca
     1861 aaacttcccg ataacgaaac tattaaaaaa cattttcata gagataactc tactcattat
     1921 catgaagaat ttatatatga atttaaaaac gttatagttg aatctgaatg cggatatata
     1981 cttgaaaatt ttcgcagaat acatcataca acctgctcta caaaaaacag actcccatcg
     2041 gtttcaaact atatttcaaa aaaaatgaga agaaaaatta tctatgaatc tgcctgtata
     2101 tttgatgcac atatcggcaa taattatttc catttttttt ctgatgttat tacaaaatta
     2161 tggatactaa agaagtataa tcttgacaga ttacctttat ttattggtca agatgtatat
     2221 tattccacta tttttcaatt tttaatttct aattctacaa tttcacaata caattggcac
     2281 gtgcttgata atcaacattt atataccata accaaacaat tatttgtttg taaacctatg
     2341 tcatacgata ttacattatg gcttcaaaca ttatctctat ttaaactacc tgttttacaa
     2401 aatagaagaa tttttgttta tagaaataaa aatacatctc gttatttata taacaccgaa
     2461 acaatttcaa aaattttaaa acaatatgga tttgaatcaa ttgatacaag caatttacca
     2521 atacgtgaac aaattagata ttttgccgaa tcaaacataa taataggagt acatggagca
     2581 ggattaacaa atatgatgtt tgcatgtcac aatcaaccaa caataataga aataataccc
     2641 gaaaatagaa tagcatgtca attttactgg atgtcaacca tgtttcaatt caactatttt
     2701 cctatattag gttcttcaat tattcaaaat gaattttcgg ttgatacaaa cgaattagaa
     2761 caaacaattt taaaaataat aacataatga aaattattat attttcgtgt tgtatttcat
     2821 tttttaattt ttagataaaa ataaacttta ttactttatt acttggttac tcatatcctc
     2881 aatcacgttc ttacttaaat tctcacaaat ctcaataact tttgcaacat ttttattata
     2941 ctttcctcgt tctcgaagta aatatatcga aaaatgaaaa tttcttctta accctaattt
     3001 tgttgaattt acaaaaaagc cttttaataa atcttttata gaatacacaa actctttaaa
     3061 ccaaaaatac ctggtaatct taggtgtata accaaacaaa aaacgtctat aaaattgagt
     3121 aacatatccc gaagaactca tacctttttg caatctttgt aaatatgtta ttgttaatcg
     3181 tttaggttca atataatgtt taaatcgtaa tgaattatta taccatatat caaagcctgc
     3241 tatttgaaat gcaagacaca tttcactatc ttcgcccgat gataaagact tccctgttct
     3301 acctaacgtg aaaaatgtaa atcctttaga aacaatatat tcccatgctt ttcgttgaac
     3361 aatcattccg gctccgtata ccacattcct aacaccaaat actctaccat tgtgtggaaa
     3421 ttgttctcca accgcataaa aattttgata ttttacaaac cattccggga atttgatatc
     3481 tgatactgct tcaccatatc ctccaagcac tgcaat
//
LOCUS       DAOTOW010000159         3513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21131_length_3513_cov_3.491324, whole genome shotgun sequence.
ACCESSION   DAOTOW010000159 DAOTOW010000000
VERSION     DAOTOW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21131_length_3513_cov_3.491324"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="P5243_03815"
     CDS             complement(<1..854)
                     /locus_tag="P5243_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS18607.1"
                     /translation="MQISVVIPLFNEEESLPELCSWIEQVMNKASFTYEIVLIDDGSR
                     DNSWQVIQQLAKKNSAIKGIKFQRNYGKSAGLFKGFEAASGDVVITMDADLQDNPEEI
                     PELYRMITEDNFDLVSGWKKKRYDPVLTKNLPSKLYNTTVRLMTGIQLHDFNCGLKAY
                     KKQVVKSIEVYGEMHRYIPVLAKWAGFKKIGEKVVQHQERKYGTTKFGIERFLRGPLD
                     LLSVLFISKFSKRPMHLFGSIGLLSFVIGALILGYLSYMKIVESVYNMTQRPLFFLGI
                     VCLIVGSQ"
     gene            977..1219
                     /locus_tag="P5243_03820"
     CDS             977..1219
                     /locus_tag="P5243_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRS18608.1"
                     /translation="MIFFDIAKPIAIIGVSRNTSKFSNYVYSDLLRKVFTVTPVNPHT
                     TSINNNTCYTQIAELPKHIDKIIILTNKKYTDSLIL"
     gene            1511..>3513
                     /gene="mutS"
                     /locus_tag="P5243_03825"
     CDS             1511..>3513
                     /gene="mutS"
                     /locus_tag="P5243_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRS18609.1"
                     /translation="MSEKIVETPLMKQYYQVKSKHPDAILLFRVGDFYETFSEDAIQT
                     SRILGITLTRRANGSASFVELAGFPFHALDTYLPKLVRAGQRVAICEQLEDPSKTKTI
                     VKRGVTELVTPGVSLSDTILNNRENNFLAAIHVSNHQVGIAFLDISTGEFLVGEGSLE
                     YIDKLISGFRPKEILFDRTKEKWFKDSFQSNLYTYKLDDWIFTTEAANDKLLSHFETS
                     SLKGFGIDIYPHAIIAAGAILHYLDITEHKQIGHIRSISRIEQDEYVWLDRFTIRNLE
                     LFSSPHEGAKTLLDVLDVTQTPMGSRLLKRWIALPLKQVNQIQQRLDIVEEFTTHESL
                     LTTVEKLLHNMCDLERIVARVATGRILPREMIQLLKTLQDIVPLQELCKSNSNQTLQR
                     YGEQLNSCEQLISKISNTIIADPSNAIQRGGVIAHGVNSELDKLREISHNGKDFILQL
                     QEREELRSGIPKLKVAFNNVFGYYIEVRNTHKDKVPADWIRKQTLVNAERYITQELKE
                     YEEQILGAEDRIVQLEQEIYIQLIQEVAAYIAILQVNASVIAHIDCLLSFATVSIQNN
                     YTKPTIDDSTTLSIINGRHPVIETKMPPGEQYVGNDVLLDTEKQQIIIITGPNMAGKS
                     ALLRQTALITLMAQIGCFVPANSAHIGIVDKIFTRVGASDNIS"
BASE COUNT         1271 a          553 c          534 g         1155 t
ORIGIN      
        1 aactgtgaac cgacaataag acaaacaata ccaagaaaaa acaatggtcg ttgagtcatg
       61 ttgtagactg attctacaat tttcatataa gataaatacc caagaataag agctccaatc
      121 acaaaactta ataaaccaat agaaccaaat aaatgcattg gtcgtttact aaattttgaa
      181 ataaataata ccgataataa atcaagcggt cctcgaagaa aacgttctat accaaacttt
      241 gtagtaccat atttacgttc ttgatgttgt accacttttt cacctatttt tttaaaacct
      301 gcccatttag caagtaccgg tatatatctg tgcatttcgc catatacctc tatgctttta
      361 actacttgtt ttttataggc ttttaatcca caattaaaat catgtagttg aatacctgtc
      421 attaatcgaa cggtagtatt atataattta cttggcaaat ttttagtcaa taccggatca
      481 tatcgttttt tcttccaacc tgataccaaa tcaaaattat cttctgtaat cattctgtat
      541 aattcgggaa tttcttcggg attatcttgt aaatcagcat ccatagttat tacaacatca
      601 ccgcttgcgg cttcgaatcc tttaaataaa ccagctgatt ttccgtaatt tctttgaaat
      661 tttatacctt ttatagcaga attttttttt gcaagttgtt gtatcacttg ccacgaatta
      721 tcacgactac cgtcatctat caaaactatt tcatacgtaa atgatgcttt gttcattact
      781 tgttctatcc aagagcatag ttcaggtaga gattcttctt cgttaaatag tggaattaca
      841 actgatattt gcatgtcata gtatttttta caaatgtata tgtttctttg attttttaaa
      901 agtttttata tcaagaatag ctgtaaaaat aatatattta tttatacaca ataacatatg
      961 gaattagata ctcgcagtga ttttttttga tatagcaaaa ccaatagcaa taattggagt
     1021 ttcaagaaat acttcaaaat ttagtaatta tgtttattct gatttattac gaaaagtatt
     1081 tactgtgact ccggtaaatc cccatactac aagcattaat aataatactt gttatacaca
     1141 aattgcagaa ttacccaagc atattgataa aattataatc ctaacgaata aaaaatatac
     1201 cgattcactc atattatgaa gcggcaaata atggaatcaa atatatttgg atacaacaag
     1261 gttgtgaaac taacgaatct atttctatag caaatacata ttccatacac attatgatac
     1321 aaagttgtgt ttgtatgtat gctaaacccg tgcaaggaat tcatgcattt catagaggca
     1381 ttttgaaatt atttggaact tataaaaagt aatataagta ggaactgtta acaaaatgtg
     1441 aaagctttca ttagcattac agaaaagagt ttctaaataa acacgtactt ttgtataaaa
     1501 tctaaaaaac gtgtcagaaa aaattgtaga aaccccatta atgaaacaat attatcaggt
     1561 aaaaagtaaa catcctgatg caatattgtt atttagggta ggcgactttt atgaaacttt
     1621 ttctgaagat gctattcaaa cttcacgtat tttaggtatt actcttaccc gacgagcaaa
     1681 tggttcagca agttttgttg agttagccgg attccccttt catgcattag atacatattt
     1741 accaaaatta gtaagagcag gacaacgagt tgctatatgt gaacaattag aagacccatc
     1801 aaaaacaaaa actattgtta aacgaggtgt tacagaattg gtaaccccgg gagtatcatt
     1861 aagtgatact attttaaata atagagaaaa taattttttg gcagcaatac atgtttcaaa
     1921 ccatcaagtt ggtattgctt ttcttgatat ttcaaccggt gaatttttag ttggagaggg
     1981 gtccctagag tatatcgata aattaataag tggatttaga ccaaaagaaa ttttgtttga
     2041 cagaacaaaa gaaaaatggt ttaaagattc ttttcaaagc aatttatata cctataaatt
     2101 agatgattgg atatttacaa ccgaagccgc gaatgataaa cttttatctc attttgaaac
     2161 atcttcgctc aaaggatttg gtatcgatat ctatccacat gctataattg cagcaggggc
     2221 tattttacac tatttagata ttacagaaca taaacaaata ggtcatattc gttctatttc
     2281 aagaattgaa caagatgaat atgtatggct cgacagattt actattcgaa atttagaact
     2341 gttttcatca cctcacgaag gagcaaaaac attacttgat gttttagacg ttactcaaac
     2401 acctatgggg tcacgattac tcaaaaggtg gattgcttta cctttgaaac aagtaaatca
     2461 aatacaacaa agattagata tagttgaaga atttacaaca catgagtcat tactaactac
     2521 agtagaaaaa ttattgcata atatgtgtga tttggaacga atagttgctc gagttgctac
     2581 aggccgtatt ttgcctcgag aaatgataca actattaaaa acattacaag atattgttcc
     2641 attacaagaa ttatgtaaat caaattcaaa ccaaaccctt caacgatatg gggaacaact
     2701 caattcttgt gaacaactta tttcaaagat ttcaaatacc ataattgccg atccgtcaaa
     2761 tgctattcaa cgcggtggtg ttattgctca tggagtaaat agcgaattag acaagttacg
     2821 agaaatttca cataatggta aagattttat attacaatta caagaacgtg aagaattgcg
     2881 aagtggtatt cctaaattaa aagttgcatt taataatgtg tttggttatt atattgaagt
     2941 aagaaatacc cataaagata aagttcctgc agattggatt aggaaacaaa ctctagtaaa
     3001 tgcagagcga tatataacac aagaattaaa agaatatgaa gaacaaatat taggtgctga
     3061 agacagaatt gtacaattag aacaagaaat ttatatacaa ttaatacaag aagttgcagc
     3121 atatattgct attttacaag taaacgcttc tgttattgca catattgact gtctattgtc
     3181 gtttgcaaca gtaagtattc aaaataatta tacaaaacca actattgatg actctaccac
     3241 tttatctatt ataaatggaa gacatccggt aatagaaact aaaatgcctc ccggcgaaca
     3301 atatgttggt aatgatgtgc tattagatac agaaaaacaa caaattatta ttattacagg
     3361 tcctaatatg gccggtaaat cagccttgct tcgtcaaact gccttaataa ctcttatggc
     3421 tcaaattggt tgttttgttc cggccaattc tgctcatatt ggtatagttg ataaaatttt
     3481 cacacgggta ggagcttctg ataatatttc tca
//
LOCUS       DAOTOW010000160         3500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21212_length_3500_cov_3.373295, whole genome shotgun sequence.
ACCESSION   DAOTOW010000160 DAOTOW010000000
VERSION     DAOTOW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21212_length_3500_cov_3.373295"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..282)
                     /locus_tag="P5243_03830"
     CDS             complement(49..282)
                     /locus_tag="P5243_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18610.1"
                     /translation="MKTIGLHTSFWVVCSAIIAIIYIPAIVQQGMFLDGVTYAAISKN
                     LSNGIGTWWKPSYTATFNSEFYGHPPFFFILQW"
     gene            complement(288..917)
                     /locus_tag="P5243_03835"
     CDS             complement(288..917)
                     /locus_tag="P5243_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRS18611.1"
                     /translation="MICVSIPVSNTDECLELIQSAAMAEIRIDMAGFDMTAVAHVFSS
                     AKKPLIATCRPEIVADNDRVKLLIHAIQHGAAYVDIEYEASPAIQQKIIAEAKKYTCK
                     VIISYHNYSTTPSTKELHTIIDSCFNAGADIAKIATTALTPNDSARILGLYDSYTNLV
                     ALAMGKLGAITRIANCYLGSPFTFAAINQEQKTAPGQFTVEEMKLFYSI"
     gene            complement(904..1938)
                     /gene="aroB"
                     /locus_tag="P5243_03840"
     CDS             complement(904..1938)
                     /gene="aroB"
                     /locus_tag="P5243_03840"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRS18612.1"
                     /translation="MKTLSIHSAIGNSDILVGETLANVTNYLPKNKKIAIITDSNIRS
                     LYGAQFPQANVVIEIPKGENNKVLHTLENIFEQLVAAEFDRTSFILAIGGGIVCDVSG
                     FAAATFMRGIEFGFVSTTLLSQVDASVGGKNGVNFKGFKNMIGTFCLPKFVICELNML
                     KTLPTSELLSGMAEVVKHGAIADADLFAYIEKHPDKVLSYDAEALERFVYDSVVIKSN
                     IVNKDAKEGGARRFFNFGHTFGHAIEKNTKMPHGYAVSIGMVIASKMSAARGLLSQHD
                     VERIIAVLQSLGLPTECEIEPIALFEALKRDKKRDGDGIHFVLLDRIGNAIVEKMSLN
                     ELEHIIHDMR"
     gene            complement(2005..3084)
                     /locus_tag="P5243_03845"
     CDS             complement(2005..3084)
                     /locus_tag="P5243_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HRS18613.1"
                     /translation="MHLNIEPIESWGVKPTKPFILSGPCSAESEEQVLTAARGLAAYG
                     VQIFRAGIWKPRTRPNQFEGVGSVGLKWLRTVKQETGMLTATEVANVKHVYEALKMGV
                     DILWIGARTSANPFSVQEIADALQGVDIPVLIKNPVNPDLELWIGAIERIAGAGITKL
                     GAIHRGFSSSEKTKYRNVPQWQVAIELHQRIPNLPIICDPSHIAGRADLVFEISQQAM
                     DLGQDGLIIESHPNPKIALSDGKQQLTPDEVGQLIKNLKIREATTDNIKFTQSLEELR
                     AKIDMIDEEILAIIQRRMNVVKEIGKNKKENNIRILQTDRWMQIIEKAKQKGNSKGLS
                     DEFIEKLFKAIHQESINIQTEILNS"
     gene            complement(3104..>3500)
                     /locus_tag="P5243_03850"
     CDS             complement(3104..>3500)
                     /locus_tag="P5243_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRS18614.1"
                     /translation="KILGMSILYMNAEEHDKHIAYVSHLTHVIAYALSLTVQEIEKDE
                     KTIFEMAGSGFGSTVRIAKSSTDMWTPIFKQNAKYLTASIDKYIDILQHFKEVIQKNN
                     DAELYEMIKEANKIRNVLDKPNMQIKTKK"
BASE COUNT         1008 a          638 c          572 g         1282 t
ORIGIN      
        1 tctcaaatca ttttactata tttacaactt gtcaaaaaag acagccattt accattgtag
       61 tataaagaaa aatggaggat gaccataaaa ttcagaatta aatgtggcgg tatagctggg
      121 tttccaccac gtgccaatac cgttagaaag attttttgaa atagcagcgt atgttacgcc
      181 atctaaaaac ataccttgct gaactattgc aggtatataa ataattgcaa taattgcact
      241 acatactacc caaaaacttg tatgtaatcc aatggttttc aatgtggtta tattgaataa
      301 aagagtttca tctcttcaac ggtaaattgt ccgggagctg ttttttgttc ttggtttatt
      361 gctgcaaaag taaaaggact ccctaaatag cagtttgcaa tgcgggtaat agctcctaat
      421 ttacccatag ctaatgctac aagatttgta tatgaatcat acaaacctaa aatacgagca
      481 ctgtcattgg gggtaagtgc tgtagttgca atttttgcaa tatcggctcc tgcattaaag
      541 caactatcta ttattgtatg caattcttta gttgatggag ttgttgagta attatgatat
      601 gaaataatta ccttacaggt atattttttt gcttcggcaa ttattttttg ttgaattgca
      661 gggcttgctt catattctat atcaacataa gctgctccat gttgtatagc gtgaataagt
      721 aatttcacac ggtcattatc tgcaactatt tcggggcggc aagtagctat aagaggtttt
      781 ttagctgaac taaatacatg agctactgca gtcatgtcaa aaccggccat atcaattcgt
      841 atttcagcca tagcggctga ttgtattagt tctaaacact catcggtatt agataccgga
      901 atactaacgc atatcatgta ttatgtgttc taattcgttt aaactcattt tttcaactat
      961 agcatttcca atcctatcaa gtaatacaaa atgaattcca tcgccatcac gttttttgtc
     1021 tcgtttaaga gcttcaaata aagctattgg ttctatttcg cattcagtag gtaatcctaa
     1081 tgattgaagt acagctatga ttctttcaac atcatgttgt gaaagtaatc cgcgtgcagc
     1141 agacattttg gaagctatta ccatacctat acttactgca taaccatgag gcatttttgt
     1201 gtttttttca attgcatgac caaatgtatg accaaaatta aaaaaacgtc gtgctccacc
     1261 ttctttggca tctttgttta caatatttga ttttataaca accgaatcgt atacaaatcg
     1321 ttctaatgct tcggcatcat atgataatac tttatcagga tgtttttcaa tataagcaaa
     1381 taaatcggca tcggctattg ctccatgttt taccacttct gccattccac ttagtaattc
     1441 cgatgttggt aaggttttaa gcatattcaa ttcgcaaatc acaaattttg gcaaacaaaa
     1501 ggtgcctatc atatttttaa agcctttgaa gtttacgcca tttttgcctc caacacttgc
     1561 atctacctga ctaagcaatg tggtagaaac aaatccgaat tctataccgc gcataaacgt
     1621 agcagcggca aatccactta catcgcatac tataccgcca cctatggcta gtataaacga
     1681 agtgcgatca aactcggctg caaccaattg ttcaaaaata ttttcgagtg tatgcaatac
     1741 tttattgttt tcacctttgg gaatttctat tactacattt gcttgaggaa attgagcccc
     1801 atacagactt cgaatattac tatcggttat aattgcaatt tttttatttt tgggtaaata
     1861 gtttgtaaca tttgccaatg tttctcctac aagtatatct gaattgccta tggcactgtg
     1921 aattgataat gttttcatta taggtttata ggtttatagg tttataggtt tacaggacta
     1981 tcccttacac ctttttaacc ttatttatga atttaaaatt tctgtttgta tattaataga
     2041 ttcttggtga atagctttga ataatttttc aataaattca tcacttaatc ctttagaatt
     2101 ccctttttgt tttgcttttt caataatttg catccaacgg tcggtttgaa gaattctgat
     2161 gttattttct tttttgtttt ttccaatttc ttttaccaca ttcattcggc gttgaataat
     2221 tgcaagaatt tcttcatcaa tcatatcaat ttttgcacgt agctcttcaa gtgattgcgt
     2281 aaattttata ttatcggtgg tagcttcacg aatttttaga ttttttatta attgacctac
     2341 ttcatcgggt gtaagttgtt gtttaccatc acttaatgca attttaggat ttgggtgaga
     2401 ttctataatt aatccgtctt gtcctaaatc catagcctgt tgcgaaattt caaatactaa
     2461 atcggcacgg cctgctatat ggctcgggtc acatatgata ggtaaatttg gaattcgttg
     2521 gtgtaattct atagcaactt gccattgtgg cacgttgcga tatttggttt tttccgacga
     2581 tgaaaatcca cggtgtatag cacctaattt tgtaatcccg gcaccggcaa ttcgctcaat
     2641 ggcacctatc cacaattcta aatcgggatt tacaggattt ttaattaata caggaatatc
     2701 aacaccttgc agtgcatcgg caatttcttg taccgaaaaa ggatttgcag aagtacgtgc
     2761 tccaatccat agtatatcta cacccatttt tagggcttcg tatacatgtt ttacattggc
     2821 aacttcagtt gctgtaagca ttcctgtttc ttgttttacg gtacgtaacc attttagtcc
     2881 tacagaacca actccttcaa attgatttgg acgtgtacgt ggtttccaaa tacctgctct
     2941 aaatatttgt acgccataag ccgctaaacc acgggctgct gttaatactt gttcttcgct
     3001 ttcggcactg cacggacctg ataatataaa aggttttgtt ggttttactc cccaactttc
     3061 aataggttca atgtttaaat gtatttccat atagtatatt caattatttt tttgttttta
     3121 tctgcatatt tggtttatca agcacattac gtattttatt tgcttcttta atcatttcat
     3181 agagttcggc atcgttattt ttttgtatta cttctttaaa atgttgtaat atatcaatgt
     3241 atttgtcgat agacgcagtt aaatatttcg cattttgttt aaaaattgga gtccacatat
     3301 cggtactact ttttgctata cgtactgtag aaccaaatcc actgcctgcc atttcaaaaa
     3361 tagttttttc gtctttttca atttcttgca ctgtgagact caatgcatac gctatgacgt
     3421 gtgtaagatg cgaaacataa gcaatatgtt tgtcatgttc ttcggcattc atgtataata
     3481 tactcatacc aaggattttg
//
LOCUS       DAOTOW010000161         3487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21294_length_3487_cov_3.709207, whole genome shotgun sequence.
ACCESSION   DAOTOW010000161 DAOTOW010000000
VERSION     DAOTOW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21294_length_3487_cov_3.709207"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..739)
                     /locus_tag="P5243_03855"
     CDS             complement(155..739)
                     /locus_tag="P5243_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18615.1"
                     /translation="MKFFISIFCVFLLYNCNCKYCTDNLSFENQKIIYSDSSLITFKN
                     DTLGVKTDTIFVFLGKISKRPYACNNGNDDKYVCHGESTIKYSNLFVLKILQFPNSFN
                     CNNCIGIGFPYEYQEFIQTDSIQYSYKNKTLQAFHTYFKQDIKDSLLYGKNISKDSLY
                     KYNNYIYVKDSIIKLLEYSITYKDGTKRKWKLKE"
     gene            complement(742..2868)
                     /locus_tag="P5243_03860"
     CDS             complement(742..2868)
                     /locus_tag="P5243_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18616.1"
                     /translation="MKKIIVILLYLCSTFVSNHMYAQTTQENLEKYWLYRDRLRKHFI
                     VISPHDEFGTNIPFFYHSFYWKEGNIDRRGLTIGDGNDGLQYYIGMLATEYALSQKYN
                     MDVTQTRNELLYALKAVERLDKYAECQFRQLPNIKKNSEDNTGTNINEIYTQIPNTNQ
                     YKDGDLNGFFIRDDIGKDFIDRYPHLKPYNTVRSTFIEGQGRNMPYEQSQDNVWNYLV
                     NLALVYILVDDAEIKKKTQDIAYRMITYMHSNVTNEVYYPCGLFSGCFPGEWRTITTP
                     TWKITNPVTGFEVVDGARIEELTTLPNGTPLPCTDVIGFNYGFAEAGNFITNKNLHYS
                     CSQDQGRENWFYTHVGFATWKSRIPHATLATVGGNNTIYSNYASGTIMQALFASLFQD
                     IGKCPSQLEHLPLIRKLIVDYQMKQGMYDNYEDLQVFNEFKNSRNLLAFYENLLNEAP
                     ICGPVNDGPGKYRVINWSVDNRLTNSGQIKELDPIESDPYRVKIDNGFNNGTYNGIDY
                     MLLHNLYWLCFAPPSEPFTVNCTNYSPNIHTRNIVSNCTIPANKTITYTASESISLQP
                     GFSAQKGSTFKAEINTQWRYQYTNPAELISSNNCYSGIPYSLQKSESITSEIYNEISP
                     YTYPSEIVDLPLYAIMPNPNTGSFTIKNIQQKNFNIQIYSMSGVLLTQHQSYGNDIPI
                     SIEQKGIFQLQITSENKVYTEQIICK"
     gene            complement(2889..>3487)
                     /locus_tag="P5243_03865"
     CDS             complement(2889..>3487)
                     /locus_tag="P5243_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18617.1"
                     /translation="GPVLYGWKGGALGIRQRTNLSSVNGPHIEKIALQWTPSDILLGN
                     SMLPMNLKTWGPIYSYNIQDNKYGIVLNGTNNARIISVTNTATNTTVFRLYGNGELRT
                     KKIWAESIDVVASVQWPDYVFKKDYALMSLQNLEYFIAQHSHLPEVPSEQQVAEQGIN
                     VAEMNAILLKKIEELTLYIIDLQKQIEATNAKVDSLDK"
BASE COUNT         1138 a          532 c          503 g         1314 t
ORIGIN      
        1 catcgcacga attatctcaa aaactcgaat tacgacaaat gacttgctgg agttttaaga
       61 aaaaactatt ggaatgtgtg caaagtcaag gcgatataag tactgacaaa aaaattgaaa
      121 tagagcgaat tatattgcag acgttgtagg tatcttactc tttcaatttc cattttcgtt
      181 tagttccatc tttataggtt atggaatatt caagcaactt aataattgaa tctttaacat
      241 aaatgtaatt gttatactta tataaagaat ctttactaat attctttcca tataacaaag
      301 aatcttttat gtcttgttta aaataagtat gaaatgcttg taaagtttta tttttatatg
      361 aatattgtat agaatctgtt tgaataaact cttgatattc atatggaaat cctataccaa
      421 tacaattatt acaattaaat gaatttggga attgtaatat ttttaaaaca aataaatttg
      481 aatatttaat agtactttcc ccgtgacaaa cgtatttgtc atcattgccg ttattacaag
      541 catatgggcg tttactaatt ttgcctaaaa aaacgaaaat tgtatcagtt ttaacaccta
      601 acgtatcatt tttaaaagta ataagactac tatcagaata tataattttt tgattttcaa
      661 aagataaatt gtcagtacaa tatttgcaat tacaattata taataaaaat acacaaaata
      721 ttgagatgaa aaatttcata attatttaca aattatttgt tcggtatata ctttattttc
      781 tgaagttatt tgtaattgaa atattccttt ttgttcgata ctaataggaa tatcatttcc
      841 atacgattgg tgttgagtca ataagacacc tgacatagaa tagatttgaa tattgaaatt
      901 tttttgttga atatttttta tagtaaaact cccggtatta ggatttggca taatagcata
      961 cagaggtaaa tcaacaattt ctgatggata tgtataagga gaaatttcat tgtaaatttc
     1021 agatgttatt gattccgatt tttgtaatga atagggaatt ccggaataac aattatttga
     1081 agaaattaat tcagcaggat ttgtatactg atacctccat tgtgtgttaa tttctgcttt
     1141 gaatgtagaa cctttttgtg ccgaaaatcc aggttgtaac gaaattgatt cgcttgctgt
     1201 atatgtaata gttttattgg ctggaatagt acaatttgaa acaatattgc gtgtatgaat
     1261 atttggagaa taatttgtgc aattcaccgt aaaaggttct gaaggtggag caaaacataa
     1321 ccaatataaa ttatgtagaa gcatatagtc aataccatta tatgttccat tattaaatcc
     1381 gttgtcaatt tttacacgat atggatctga ttcaatagga tctagttctt ttatttgtcc
     1441 actattagtt aatctattat caacactcca gttaattact cgatacttac ctgggccatc
     1501 atttaccgga ccacaaatag gagcttcgtt cagtaaattt tcataaaaag caagtaaatt
     1561 tctcgagttt ttaaattcat taaatacttg taaatcttca taattatcgt acatgccttg
     1621 cttcatttga taatctacta taagtttgcg tatgagaggt aaatgttcaa gttgtgaagg
     1681 gcatttacca atatcctgaa ataacgaagc aaataatgct tgcattatag tgccggatgc
     1741 ataatttgaa tatatagtgt tattaccgcc aactgttgcc aaagtagcat gaggtatgcg
     1801 agatttccaa gtagcaaatc caacatgagt ataaaaccaa ttctcgcgac cttgatcttg
     1861 agaacaacta taatgtaaat ttttattggt aataaaatta cctgcttcag caaatccata
     1921 attaaaacct atcacgtcag tacatggtaa tggagtgcca tttggtaatg tagttaattc
     1981 ttcgattcgt gcaccatcta caacttcaaa ccctgttacc ggattggtaa ttttccaagt
     2041 tggggtagtt atagttctcc attcaccagg gaaacaacca ctaaaaagac cacatgggta
     2101 ataaacctca tttgttacat ttgaatgcat atatgtaatc attctatacg caatatcttg
     2161 tgtttttttc ttgatttcag catcgtcaac taatatatat actaaagcta aattaaccaa
     2221 ataattccat acattatctt gactttgttc atagggcatg tttcttcctt gcccctcaat
     2281 aaacgtcgaa cgtactgtat tatatggttt taaatgagga tatctgtcaa taaaatcttt
     2341 tccaatatcg tcgcgaataa aaaaaccgtt caaatcgcca tctttatatt gattagtatt
     2401 tggtatttgc gtataaattt cgtttatgtt tgtacctgta ttatcttctg aattcttttt
     2461 tatgttaggt aattgtctga attgacattc tgcatatttg tcaagtcgtt caacggcttt
     2521 taatgcatac aataattcat tacgtgtttg agttacatcc atattatatt tttgtgacag
     2581 agcatattcc gttgctaaca ttcctatgta atattgtaat ccatcatttc catctccaat
     2641 ggttaagcct cttctatcaa tattaccttc tttccaataa aatgaatgat aaaaaaaggg
     2701 aatatttgtt ccaaattcat catgagggct aattactata aaatgttttc gcagtctatc
     2761 tctgtatagc cagtattttt ctaaattttc ttgtgtagtt tgagcataca tgtgattact
     2821 tacaaaagtg ctacacaaat atagtagtat tacaattatt tttttcatta tttctttaga
     2881 attacatgtt atttatctaa actatcaact tttgcatttg tagcttcaat ttgtttttgt
     2941 aaatcaataa tatacaatgt gagctcttct atttttttaa ggagtatcgc attcatttcg
     3001 gctacattta taccttgttc agctacttgt tgttcggaag gtacttcagg taaatggcta
     3061 tgttgtgcta taaaatattc taaattttgt aaactcatta atgcataatc ttttttgaaa
     3121 acataatcag gccattgtac acttgcaaca acatctatag attctgccca tattttttta
     3181 gttcgtaatt ctccatttcc atacaatcta aacacggttg tatttgttgc agtatttgtt
     3241 acgcttatga ttcgagcatt attagttccg tttaaaacta ttccatattt attgtcttgt
     3301 atattataac tgtagattgg tccccatgtt tttaaattca ttggcaacat tgaatttcct
     3361 agtaaaatat cacttggagt ccattgtaaa gcaatttttt ctatatgtgg accatttaca
     3421 gaacttaaat tagttcgttg ccgtattcca agtgctccac ctttccatcc atataataca
     3481 ggtccat
//
LOCUS       DAOTOW010000162         3484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21314_length_3484_cov_3.807232, whole genome shotgun sequence.
ACCESSION   DAOTOW010000162 DAOTOW010000000
VERSION     DAOTOW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21314_length_3484_cov_3.807232"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="P5243_03870"
     CDS             <1..276
                     /locus_tag="P5243_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRS18618.1"
                     /translation="AEELWQIYGNKQTLAYEPWPQFNPAMLEEDTVQYPVSFNGKKRF
                     ELELPKTMSKQEVETAALSDERSAKWLVDGKAKKIIVVPGKIINIVV"
     gene            complement(413..1249)
                     /locus_tag="P5243_03875"
     CDS             complement(413..1249)
                     /locus_tag="P5243_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS18619.1"
                     /translation="METIVPKLDTILVPTDFSEVGNAAVQQAVEIAKLHAGVIHLLHV
                     VETKKNETVEECLSLLKQSAQTSSYTQFKYIIEEGDIFSTIDAVAERINADLIIMGTH
                     GKVGFQKLLGSNALKVIDSTKVPLMVVQNKVITGGFKNILFPVSLADEDRQKTTIAIR
                     LAKMFNSTIHIFPRYESTKTGEKQMISTILQIKAYFTKYDVSFEVAPLLTSSDNFEKK
                     ILDYSAQISADLIIIMSDSDDHFIPLFGSKEEYVLFNSMKIPVICVEEKKIKKVRFST
                     AG"
     gene            1608..2867
                     /gene="lysA"
                     /locus_tag="P5243_03880"
     CDS             1608..2867
                     /gene="lysA"
                     /locus_tag="P5243_03880"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRS18620.1"
                     /translation="MTKTVPFNKAKIEEIIQTYPTPFHIYDEAAMIANAQQLQAAFSW
                     NEGFKEFFAVKAAPNPYLIKILSQVGFGCDCSSYPELLLSESAGVVGENIMFTSNNTA
                     AYEYKKARELGAVINLDDISHIAYLEEHAGLPDLLSFRYNPGNLKEGNDIIGSPEEAK
                     YGFTEAQLFEGYKILQQKGIKRFGIHTMVASNELNNDYFIETAEIMFKLIVKLHTQLG
                     IRFEFVNLGGGIGIPYRPDQKPVDIQYISNGIKKKFEEIIVANGLAPLKIFLESGRYI
                     TGPYGYLVTRVQHFKQTYRDYVGVDASMSNLMRPGMYGAYHHITVLGKEFAPKIHTYD
                     VTGSLCENNDKFAIHRELPKIEIGDILVIHDTGAHGHAMGFNYNGKLRSAELLLRTDG
                     SVVQIRRAETVEDYFATLDFSGVSSFK"
     gene            2876..>3484
                     /locus_tag="P5243_03885"
     CDS             2876..>3484
                     /locus_tag="P5243_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS18621.1"
                     /translation="MQKKPRILIVDDQYSNRFLMAEMLLDYDIAMATSGTEMWEEITK
                     KVPDLILLDVMMPEEDGFSLAKKLHAHKDYKTIPVIFVTAKVTGADIDEGFRSGAYDY
                     IKKPFNQQELETRVKNALENSAKTHDLLARAITGDMIFENMPESLIITDKDFRIVSIN
                     PACSALTGYLNSDVRESFFPDYICDESQNPIAIGKLLHAKHEV"
BASE COUNT         1170 a          498 c          663 g         1153 t
ORIGIN      
        1 gccgaagaat tgtggcagat atatggtaac aaacaaacac ttgcatatga accatggcct
       61 caatttaatc ctgcaatgct cgaagaagat accgtacaat atcctgtgtc gtttaatggt
      121 aaaaaacgat ttgaattgga attgccaaaa actatgagta aacaagaggt agaaactgca
      181 gctctcagtg atgaacgttc tgctaaatgg cttgttgatg gaaaggcaaa gaaaataatt
      241 gtagtgcccg gaaaaattat aaatattgtt gtgtaacaac aaatgaaatt atatgcataa
      301 aaaaagctga atataaaata ttcggctttt tttatgaatt atagttgtaa gtaattataa
      361 aaatttcaac tttttcggaa ttgaaatatt tttgaacaaa tatttgaaaa aactatcccg
      421 cagtagaaaa tcgtactttc ttaatttttt tctcttctac acaaattacc ggaattttca
      481 tgctgttaaa caacacatat tcttcttttg agccaaacag aggtataaaa tgatcgtcgg
      541 aatcactcat tataattatt aaatcggcgc taatctgtgc actatagtct aatatttttt
      601 tctcgaaatt gtctgatgag gtaaggagtg gagcaacctc aaacgaaaca tcgtatttcg
      661 taaaatatgc tttgatttgt aaaatggttg aaatcatttg tttttcgcct gttttggtag
      721 attcgtagcg agggaaaata tgtatggtag aattaaacat ttttgcaaga cgaattgcaa
      781 tagtcgtttt ttggcgatct tcatctgcta aacttaccgg gaataatata tttttaaacc
      841 cacctgttat taccttgttt tgaacaacca taagaggtac tttggtgctg tcaattactt
      901 taagagcatt gctcccaagt aatttttgaa accctacttt accgtgagtt cccatgataa
      961 taaggtcggc atttatgcgt tcggcaacgg catctattgt agaaaatata tcaccttctt
     1021 ctattatata tttgaattgt gtgtacgaac ttgtttgtgc agattgttta agtagactta
     1081 ggcattcttc aacagtttca ttttttttgg tttcaaccac atgaagcaaa tgaattacgc
     1141 cggcatgtaa tttagctatt tctactgctt gttgtactgc ggcatttcca acttcgctaa
     1201 agtctgttgg aacgagaata gtgtctaatt ttggaacaat tgtttccata atgtttttaa
     1261 atttagtatt gtttttcttc gcacaaactt acagtaaaat ggagtaaatc gcaatatgat
     1321 ttgcgatttt gataaattat cgtagatgat tgatgttttt ttattgatgt gaaattgtga
     1381 tagtggtgat ttatgtttgc atgtttattt gcagattttt ttaatgaata ttgcatacca
     1441 tttttattga ttattttata aaatatcagt agtattgcaa aaggtaaaat acgagggatt
     1501 ttttataata aataaagttt taggttttta aacgtattga aaatcaaaaa tatatagggt
     1561 ttttatcagg aattattgtt ggtagaatta aatgaagaaa ttaaaatatg actaaaacag
     1621 taccgtttaa taaagcaaaa atagaagaaa ttattcaaac atatccaaca ccgtttcata
     1681 tatatgatga ggcagctatg atagctaatg ctcaacaatt gcaagctgca ttttcgtgga
     1741 atgaagggtt caaagaattt tttgcagtaa aagcagctcc aaatccatat ttaattaaaa
     1801 ttttatcaca agttggattt ggttgtgatt gtagttcgta tcccgaattg ttgttgtcgg
     1861 aatctgccgg agtagttggt gaaaatatta tgtttacttc gaacaatact gcagcatatg
     1921 aatataaaaa agcacgtgaa ttaggtgcgg ttataaatct tgatgatata agccatattg
     1981 catatttaga agaacatgca ggcttgcccg atttgctttc gtttcgctat aaccccggga
     2041 atttaaaaga aggaaatgat ataataggaa gtccggaaga agcaaaatat ggttttaccg
     2101 aagcgcaatt gtttgaaggc tataaaatat tacaacaaaa aggaattaaa cggtttggta
     2161 tacataccat ggtggcttca aatgagctta acaacgatta ttttatagaa actgccgaaa
     2221 ttatgtttaa actcattgtg aaacttcata cacaattggg tattcgattc gaatttgtaa
     2281 accttggcgg tggtataggt attccttaca gacctgacca aaagcccgtt gatatacagt
     2341 atataagcaa tggaattaag aaaaaattcg aagaaataat tgtagcaaat ggtttagcac
     2401 cgcttaaaat tttcttggaa tcgggacgat atataacagg gccatatgga tatttggtaa
     2461 cacgagtaca gcattttaaa caaacatatc gcgactatgt aggtgtagat gcaagtatgt
     2521 cgaaccttat gcgtccggga atgtatggtg cgtatcatca tattactgtg ttgggtaaag
     2581 aatttgcacc aaaaatacat acttatgatg taaccggttc gttgtgcgaa aacaatgata
     2641 aatttgcaat tcatcgtgaa ttacctaaga ttgaaattgg tgatatactt gtaatacatg
     2701 atacaggagc tcatggtcat gctatgggat ttaattacaa cggtaaatta cgttcggctg
     2761 aattgttgtt gcgtaccgat ggtagtgtag ttcaaattcg aagagccgaa acagttgaag
     2821 actattttgc aacgcttgat ttttcgggag tttcatcatt taaataaatt gaaatatgca
     2881 aaaaaaacca cgaatactca tagttgatga tcaatattcc aacagatttc tgatggcaga
     2941 aatgctgttg gattatgata ttgctatggc taccagtggt accgaaatgt gggaagaaat
     3001 aaccaaaaaa gttcctgatt taatattgct cgatgtaatg atgcccgaag aagacgggtt
     3061 ttcgttagca aaaaagttgc atgcacacaa agattataaa acgattcctg taatatttgt
     3121 aacagctaaa gttaccggag ccgatattga cgaaggtttt cgcagtggtg cttacgatta
     3181 tatcaaaaag ccgtttaatc aacaagaatt ggaaacgcgt gtaaaaaatg cattagaaaa
     3241 ttcggctaaa actcatgatt tattggcacg agctattacg ggtgatatga tttttgaaaa
     3301 catgccggaa tcgttgatta taaccgataa agattttaga attgtatcta taaatcccgc
     3361 atgttctgct cttaccggat atttgaattc tgatgtgaga gaaagttttt tccctgatta
     3421 tatttgtgat gaatcgcaaa atcctatagc aattggtaaa ttgttgcatg caaaacatga
     3481 ggtg
//
LOCUS       DAOTOW010000163         3472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21411_length_3472_cov_3.338601, whole genome shotgun sequence.
ACCESSION   DAOTOW010000163 DAOTOW010000000
VERSION     DAOTOW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21411_length_3472_cov_3.338601"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="P5243_03890"
     CDS             <1..683
                     /locus_tag="P5243_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18622.1"
                     /translation="VKNNNMQLVKFVLFVCAFVIISVCNAQQLISIPLQTTIDTTTIG
                     NAIQLLQKGDQSIQIPPIITPLKIADSIVSNANDVDTLDVLHDTVQEIVQEFINPENI
                     SISKIITGEYWIGNTKHTDIMIDSSIISKLVIENITCSYLSISALSVTQISIKNSVIS
                     EFEIEDSSIGELIIENTKITDFSTDQSVIRKQTIISKPQKYEVKQQSSKSVVQPTNTI
                     DTDDDDDE"
     gene            complement(689..3412)
                     /locus_tag="P5243_03895"
     CDS             complement(689..3412)
                     /locus_tag="P5243_03895"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRS18623.1"
                     /translation="MDEVQDEAIQSKQDSDIQHIKYISGQFEDWFLDYASYVIMERAV
                     PYINDGLKPVQRRILHSMKRMDDGRYNKVANIIGHTMQFHPHGDSSIGEALVGLGQKE
                     LLIDTQGNWGNIYTGDSAAAPRYIEARLSKFANEVVFNPKTTIWKSSYDGRNKEPITL
                     PVKFPLLLAQGVEGIAVGMACKILPHNFNELIDASIKYLQGKPFELYPDFPTGGYIDI
                     TKYNDGLRGGVVKVRAKIVKIDNKTLAITEIPYGKTTTAVIDSIIKANEKGTIKIKKI
                     DDNTAANVEILIHLPPNVSPDETIDALYVFTECEISISPNASVIIEKSPEFIGVSDIL
                     RISTDKTVELLRRELEIRLEELKEDWMKTSLEKIFIEKEVYELIKPCKTEEEILSTIF
                     KGLEPHVKKFWRPVTREDCVILSNIPIKRISKFSSFKADEYIKEIEIEMDEVKNHLAH
                     MIDFAINYFTQIQKKYGKDKERKTEIRNFEQIEATQVVIANTKLYANLEDGFVGTSLK
                     KDTFICDCSDIDDIIVFMQDGKYKISKVSDKAFFGKNIIHIGVFRKNDERTIYNVAYR
                     DGKSGITYMKRFAVTGITRDKIYDVTKGTPNSKVLYFSANPNGEAEIIKVYLRQNPRL
                     RKTIFEVDFSTQVIKGRNSQGNILTKHPCFKIIKIEEGVSTLGGRKIYFNFETKRLND
                     GGYGEYLGEFKGSDKILVLTADGKYTLTTFDLSNHYEYEILKIEKFDDSKIWTAVFYD
                     GEQENYYIKRFEIEESSKFISFIGEHEKSYLVAVNDDVYPQLQIEFGGKHASREPELI
                     DVDEFIGVKGVKARGKRITTFTVKTITFIEPLEKIASSDDTIDPESEIGEEDSSDDTI
                     EPESDSEIDSENTMVETTNDEKININTLSSVKPKKEPPQNSSNTPSQMSLF"
BASE COUNT         1098 a          571 c          503 g         1300 t
ORIGIN      
        1 ttgtaaaaaa taacaacatg caattagtga aatttgtttt attcgtttgt gcttttgtaa
       61 ttataagtgt atgtaatgct caacaattga taagtattcc attgcaaacc acaattgata
      121 caacaactat tggtaatgca atacaacttc tgcaaaaagg tgatcaaagt attcaaatac
      181 cacctattat aactccttta aaaattgctg atagtattgt atcaaatgca aatgatgtag
      241 atactctcga tgtgttacat gacaccgtgc aagaaatagt tcaagaattt ataaatcctg
      301 aaaatatatc tatttctaaa attataaccg gtgaatattg gataggtaat actaaacata
      361 ccgatattat gattgattct tcaattattt caaaattggt aatagaaaat attacatgtt
      421 cttatttgag tatcagtgct ctgtctgtta cgcaaattag tataaaaaat tcggtaattt
      481 ctgaatttga aattgaagat tcttcgatag gtgaattaat aattgaaaat actaaaatta
      541 cagatttttc aactgatcaa agtgttattc gtaaacaaac aataatttca aaacctcaga
      601 aatatgaagt aaaacaacaa tcatcaaaat cggtggtgca acccacaaat accattgata
      661 ctgacgacga tgatgatgaa tagaatatct aaaataacga catttgactt ggcgtatttg
      721 atgaattttg aggcggttct tttttaggtt ttacagatga taacgtattt atatttattt
      781 tttcatcatt tgttgtttct accattgtat tttcagaatc tatttcagaa tcactttcag
      841 gttcaatagt gtcatcagaa gagtcttctt ctccaatttc tgattcagga tcaattgtgt
      901 cgtctgaaga tgcaattttt tctaatggtt caataaatgt tattgttttt acagtaaagg
      961 tagtaatacg tttaccacga gcttttacac cttttacacc aataaattca tctacatcaa
     1021 ttaattcagg ttcgcgagaa gcatgtttgc ctccaaattc tatttgtagt tgtggataca
     1081 catcatcatt tacagctact aaatatgatt tttcatgttc accaataaaa cttatgaatt
     1141 tacttgattc ttcaatttcg aatcgtttta tataataatt ttcttgttca ccgtcgtaga
     1201 acacggctgt ccaaatttta gaatcatcaa atttttcgat ttttagaatt tcatattcgt
     1261 agtgattact taaatcaaaa gtggtaagag tatacttgcc gtcggctgta agaaccaata
     1321 ttttgtctga ccccttaaat tcccctaaat attcaccata accaccatca tttaatcgtt
     1381 ttgtttcaaa gttaaaatat atttttcttc cacctaaagt agatacgcct tcttctattt
     1441 taattatttt gaagcatgga tgctttgtta aaatatttcc ttgtgaattt cttcctttaa
     1501 taacttgtgt gctaaaatca acttcaaaaa tagttttgcg taaacgagga ttctgacgta
     1561 aatacacttt tataatttcg gcttcaccat taggatttgc actaaaatat aagacttttg
     1621 aattaggtgt tcctttagta acatcataga ttttgtcacg agttatgccc gttacagcaa
     1681 atcgtttcat ataagttatt cctgatttac catcgcgata agcaacgttg taaattgtac
     1741 gttcatcatt tttcctgaat acaccaatat gtatgatatt ttttccaaaa aatgctttgt
     1801 cactcacttt tgatattttg tatttaccgt cttgcataaa tactataatg tcatcaatat
     1861 cagaacaatc gcaaataaat gtgtcttttt tgagcgaagt gccaacaaaa ccatcttcta
     1921 aatttgcata taatttagtg ttagctatta caacttgagt tgcttctatt tgttcaaaat
     1981 ttctgatttc tgttttgcgt tctttgtctt ttccgtattt tttttgaatt tgagtaaaat
     2041 agttgatagc aaaatctatc atatgagcaa gatgattttt tacttcatcc atttcaattt
     2101 caatttcttt aatatactca tcggctttaa acgaactgaa tttagatatt cgtttaatag
     2161 gaatatttga aagaatcacg caatcttcgc gagttaccgg acgccagaat tttttgacat
     2221 ggggttctaa acctttaaaa attgtgctaa gaatttcttc ttctgtttta caaggtttaa
     2281 taagttcgta tacttctttt tcaataaata ttttttcgag cgaagttttc atccaatctt
     2341 ctttgagttc ttctaaacga atttctaatt cacgacggag taattctaca gttttgtcgg
     2401 ttgatattcg taatatgtca gaaacaccaa taaattcagg agatttttct attataacac
     2461 ttgcatttgg agaaattgaa atttcacatt cggtaaaaac atataatgcg tcaatagttt
     2521 cgtcgggcga aacatttggc ggaaggtgaa ttagtatttc aacattagca gcggtattat
     2581 catcaatttt tttaatttta atagtacctt tttcatttgc ttttattata gaatcaatta
     2641 cggcagtagt agttttgcca tatggaattt ctgtaatagc caatgtttta ttgtcaattt
     2701 ttacaatttt tgcacgaact tttactacac cacctctgag tccgtcatta tattttgtaa
     2761 tatctatata accacctgtc ggaaagtcgg gatacaattc aaatggtttg ccttgtaaat
     2821 attttatact ggcatctata agttcattaa aattatgagg taaaatttta catgccattc
     2881 ccacagctat accttcaacc ccttgagcta aaagtaacgg aaattttacc ggtaaggtaa
     2941 taggttcttt gtttctaccg tcgtatgatg atttccaaat agtggttttc ggattaaaaa
     3001 caacttcatt tgcaaatttt gataatcgtg cttctatata acgaggtgcg gcagcactat
     3061 cgccggtata aatatttccc cagttacctt gggtgtcaat caataattct ttttgtccta
     3121 atccaaccaa agcttcacct attgatgagt ccccatgtgg gtgaaattgc atggtatgac
     3181 caattatgtt tgcaacttta ttgtatctgc catcgtccat tcgtttcata gaatggagta
     3241 tacgtcgttg caccggtttt aaaccgtcgt ttatatatgg tacagcacgc tccattatta
     3301 catacgatgc ataatcaaga aaccaatctt caaattgacc cgatatgtat tttatatgct
     3361 gtatgtcaga atcttgcttg gattgtattg cttcgtcttg aacctcgtcc atatatattt
     3421 tttacaaaaa ttataaaaat cattcaaagg tcataaaaac aattaaaatt ct
//
LOCUS       DAOTOW010000164         3454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21542_length_3454_cov_3.597823, whole genome shotgun sequence.
ACCESSION   DAOTOW010000164 DAOTOW010000000
VERSION     DAOTOW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21542_length_3454_cov_3.597823"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="P5243_03900"
     CDS             complement(<1..420)
                     /locus_tag="P5243_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRS18624.1"
                     /translation="MQLCYLLGYIPGSLHYYRKAFTHKSAVNSQTKFKQYNERLEFLG
                     DSIIDTIIADYLFHKYPHEPEGFLTKMKSKIVNRKSLNQIAVHMHLDSYLQSSILNVK
                     ENDAIGNAFEALIGAMYLDKGYTFTKKYFITNVIEKFF"
     gene            complement(490..1743)
                     /gene="fabF"
                     /locus_tag="P5243_03905"
     CDS             complement(490..1743)
                     /gene="fabF"
                     /locus_tag="P5243_03905"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRS18625.1"
                     /translation="MELKRVVVTGIGAFTPIGNTVEEIFKNLTNGVSGSAPITYFNTE
                     KFKTKFACEVKNFDISLYVDRKEARKMDPYAQYAMAAAAECLQDSQLQLDNIDLDSIG
                     VIWGSGIGGLSTFQTEITAFAQGDGTPRFNPFFIPKMISNIAAGYISIRYGLRGASYA
                     TTSACASSTHAMIDAFNYIRLGKAKMYITGGSEATINESGVGGFNSMKALSENNEEYA
                     SASRPFDVTRDGFVMGEGGVALLFEEYDHAIARGAKIYAEVVGGGMSSDAHHLTAPHP
                     EGLGAANVMKYAIEDAGISLTDIDYINVHGTATPLGDIAETNAIIKLFGDHAYKLNIS
                     STKSMTGHLLGAAGAMETVASIFALQTGIIPPTINFKEADPNIDSKLNLTLNQAQERK
                     VEYVLSNTFGFGGHNGSIILKKFKG"
     gene            complement(1769..2005)
                     /locus_tag="P5243_03910"
     CDS             complement(1769..2005)
                     /locus_tag="P5243_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRS18626.1"
                     /translation="MSEIASKVQAIIVDKLGVDASEVTPEANFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDEQAEGIATVGDAISYIEKNSK"
     gene            2188..2760
                     /locus_tag="P5243_03915"
     CDS             2188..2760
                     /locus_tag="P5243_03915"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRS18627.1"
                     /translation="MPKIAIFASGSGTNTRRIIEYFTHSDIEISRIYTNNAQAGVVEI
                     AHNSNIPVCIFNKSELYESTIVLNQLQNDNISCIVLAGFLLLMPANIIQAFEKRIINI
                     HPALLPKYGGKGMYGMHVHRAVVTNKETKTGITIHVVDEIYDNGTILFQAECTVLPHD
                     TPETIAQKVHELEYEHFPRVIQEYVVLLHK"
     gene            2779..>3454
                     /locus_tag="P5243_03920"
     CDS             2779..>3454
                     /locus_tag="P5243_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS18628.1"
                     /translation="MKYSKSIEAFKQAVTVIPGGVNSPVRSCKSVGGNPVFIQSAQGA
                     YITDIDGNSYIDYVGSWGPMILGHAHPQVIEAIYKTALQGTSFGAPTVIETEMAQLIC
                     NMMPNIESVRMVNSGTEATMSALRLARGYTKRDKVIKFEGCYHGHHDSFLIQAGSGAL
                     TLSTPNSPGVTASTAQDTILAQFNNFESVHNAFRAYGDQIAAIIVEPIPGNMGVVIPE
                     IKFLQQL"
BASE COUNT         1127 a          617 c          555 g         1155 t
ORIGIN      
        1 aaaaaacttc tcaattacat ttgtaataaa atattttttt gtaaacgtat aacctttatc
       61 caaatacatt gctccaatga gagcttcaaa agcgtttcct atagcatcat tttctttaac
      121 attgagaata cttgattgta aataagaatc taaatgcata tgaacagcta tttgattcaa
      181 agattttctg ttcacaattt ttgatttcat tttggtaaga aatccttcgg gttcatgagg
      241 atacttatga aaaagataat cggcaataat agtatcaata attgaatcgc ccaaaaattc
      301 aagacgttca ttgtactgtt tgaatttagt ttgagaattt acagccgatt tgtgagtaaa
      361 agctttacga taataatgta atgaaccggg aatatatcca agcaaataac ataattgcaa
      421 aaaaaactcc ttttctgcag gtttacaaaa aaggagtctt atgtatttta aaattcgcac
      481 aaaaaagaat tatcctttga attttttaag aattatagaa ccattgtgac caccaaatcc
      541 aaatgtatta ctcaacacat actcaacttt tctttcttga gcctgattaa gcgttaaatt
      601 taatttgctg tcaatattag gatctgcttc tttaaaattg atagttggag gaattattcc
      661 tgtttgtaat gcaaaaatag aagctactgt ttccatagca ccggcagcac caagtaagtg
      721 tccggtcata gatttggtag aactaatatt taatttatac gcatgatcac caaataattt
      781 gataattgca tttgtttcgg caatatctcc aagaggagta gctgtaccgt gaacattaat
      841 ataatcaata tcagttaatg aaatacctgc atcttctatt gcatatttca ttacgttggc
      901 tgcaccaagt ccctcggggt gaggtgcagt aagatgatga gcatctgaac tcataccacc
      961 gccaacaact tcggcataaa tttttgctcc acgagcaatt gcatggtcat attcttcaaa
     1021 caacaatgca acaccacctt cacccataac aaatccgtca cgagttacat caaacggacg
     1081 tgaagctgaa gcatattctt cgttgttttc agacaacgct ttcattgaat tgaaaccacc
     1141 tactccactt tcattaattg ttgcttctga cccccctgta atatacattt tagctttacc
     1201 caagcgaata tagttaaatg catcaatcat agcatgtgta gatgatgcac atgcagacgt
     1261 ggttgcataa ctagcacctc gcaatccata ccgaatcgaa atataacctg ctgctatatt
     1321 cgatatcatt ttaggtataa aaaacgggtt aaaacggggc gttccatcac cttgtgcaaa
     1381 tgcagtaatt tctgtttgga atgtagataa accaccaatt cccgaccccc aaattactcc
     1441 tattgaatca aggtctatat tgtctaattg taattgtgaa tcttgtaggc attccgctgc
     1501 ggcagccata gcatattgtg catatggatc catttttcgg gcttccttac ggtctacata
     1561 taaagaaatg tcaaaatttt taacctcaca ggcaaatttt gttttaaact tctcagtatt
     1621 gaagtaagtg ataggtgcac taccacttac tccatttgtg agatttttaa aaatttcttc
     1681 tacagtattt ccaattggag tgaaagcacc gataccggtt accactactc tttttaattc
     1741 catagattga aaataaagtg aataatgatt acttcgagtt tttctcgatg taagaaattg
     1801 cgtcaccaac tgtagcaata ccttcggctt gctcatctgg aatagcaatg ttgaattctt
     1861 tttcgaattc cataattaat tctacagtgt ctaatgagtc tgctcctaaa tcgttagtaa
     1921 aattagcttc aggagtaact tcgctagcgt caacacctaa cttgtctaca atgattgctt
     1981 gtacttttga tgcaatttct gacatagttt ttacattttt agttaataat aattttaaat
     2041 taattttatg ctgcaaataa ctatattttt ttttattcaa aaaagaaaaa actaataaaa
     2101 tttttttgtt aattttttct gttttctgat aatcaataat ttatattttt gcactctgta
     2161 agttattaac aagttattca ttattttatg ccaaaaattg caattttcgc ctcaggttca
     2221 ggtactaata ctcgaagaat tatagaatat tttactcatt ccgatattga aatttcaaga
     2281 atttatacaa ataatgccca agccggagtt gttgaaatag cacataacag taatattcct
     2341 gtttgtattt ttaacaaatc ggaattgtat gaatcaacta ttgttttgaa tcaattacaa
     2401 aacgacaata tttcatgtat tgtgttagca ggttttttac tgcttatgcc agcaaacata
     2461 attcaagctt ttgaaaaaag aattattaat attcatcctg ccttactccc taaatatggt
     2521 ggcaaaggaa tgtatggcat gcatgtgcac cgtgctgttg taaccaataa agaaactaaa
     2581 accggtatta caatacatgt agttgatgaa atatatgaca atggtactat tctgtttcaa
     2641 gccgaatgta ctgtattacc acacgatact cccgaaacta ttgcacaaaa agttcatgaa
     2701 ttagaatatg aacattttcc tcgtgtaatt caagaatacg ttgtattatt gcataaatag
     2761 taaatattat aaaagaacat gaaatattca aaaagtattg aagcgtttaa acaggctgtt
     2821 actgtaattc cgggtggagt aaattctccg gttcgttcat gcaaaagtgt tggaggtaac
     2881 cctgtgttta ttcaatctgc tcaaggagca tatattaccg atattgatgg taattcttac
     2941 atagactatg ttggttcttg gggacctatg attttaggac atgctcaccc acaggtaatt
     3001 gaagctatat ataaaactgc tttacaagga acaagttttg gagcacctac agtaattgaa
     3061 acagaaatgg ctcaacttat ttgcaacatg atgccaaaca tagaatctgt gcgcatggta
     3121 aattcgggta cagaagcaac tatgagtgct ttgcggcttg ctcgtggtta taccaaacgc
     3181 gataaagtta ttaaattcga aggttgttat cacggtcatc atgatagttt ccttattcaa
     3241 gcaggctcag gtgcattaac cctcagcact cccaattcgc cgggagtaac ggcgtctaca
     3301 gctcaagata ccattcttgc tcagtttaat aattttgaat ctgttcataa tgcttttaga
     3361 gcatacggcg accaaattgc agctattata gttgaaccta ttcccggcaa tatgggtgtt
     3421 gtaatacctg aaattaaatt tttacaacaa ttac
//
LOCUS       DAOTOW010000165         3449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21584_length_3449_cov_3.035062, whole genome shotgun sequence.
ACCESSION   DAOTOW010000165 DAOTOW010000000
VERSION     DAOTOW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21584_length_3449_cov_3.035062"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /gene="rplQ"
                     /locus_tag="P5243_03925"
     CDS             complement(<1..458)
                     /gene="rplQ"
                     /locus_tag="P5243_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRS18629.1"
                     /translation="MRHGKKVNHLGRTHEHRAAMLSNMATSLIMHKRIKTTLAKAKAL
                     QKYVDPLITKSKEDTTHSRRIVFKYLQNKFAVTELFREISSKIAERPGGYTRIIKLGN
                     RLGDAAEMCYIELVDFNDTYLKEGKTTSTKKSPVRRSRKKSTSTSEKVVNT"
     gene            complement(471..1466)
                     /locus_tag="P5243_03930"
     CDS             complement(471..1466)
                     /locus_tag="P5243_03930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRS18630.1"
                     /translation="MAILAFQKPDKVIMLESDDNIGIFEFRPLEPGYGITVGNAIRRV
                     LLSSLEGYAITNIKIEGVDHEFSTIKGVVEDVTEIILNIKQIRFKRVTTDSVQSEKVS
                     VTVAGKDKFVAGDLNAFLTNFQVLNTDLVICNMNTSVKLTIDFWIGKGRGYVPAEENK
                     IMDSEFGIIPIDSIFTPIKNVKYSVENYRVEQKTDFEKLILEIKSDGSIHPKDALKDA
                     AKILIQHFMLFSDEKITLDTEDKFGNEEFDEEVLHMRQLLKSKLVDMDLSVRALNCLK
                     AADVETLGELVQFNKNDLLKFRNFGKKSLTELDELLEALNLTFGMDISKYKLDKE"
     gene            complement(1489..2097)
                     /gene="rpsD"
                     /locus_tag="P5243_03935"
     CDS             complement(1489..2097)
                     /gene="rpsD"
                     /locus_tag="P5243_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRS18631.1"
                     /translation="MARYTGPTSKIARKFGEPIYGPDSALQKKNYPPGQHGLSKRKAK
                     KSVYGVQLQEKQKAKYMYGVLERQFANLFHEAERRPGVTGEILVQLLEQRLDNVVYRM
                     GIANTRRAARQLVTHQHITVNGAVVNIPSFHVKPGDIVAVREKSRSLEAITRSLSTNS
                     VSKFSWLEFDKSQMQGKFLNVPERSEIPENIKEQLIVELYSK"
     gene            complement(2118..2504)
                     /gene="rpsK"
                     /locus_tag="P5243_03940"
     CDS             complement(2118..2504)
                     /gene="rpsK"
                     /locus_tag="P5243_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRS18632.1"
                     /translation="MAKKTKSVKKVVKVEAAGQAHIHASFNNIIVTITNAEGQVISWS
                     SSGKKGFRGSKKNTPYAAQMAATDCAKEAFDLGLRRVKVFVKGPGVGRESAIRTIHNS
                     GIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            complement(2521..2898)
                     /gene="rpsM"
                     /locus_tag="P5243_03945"
     CDS             complement(2521..2898)
                     /gene="rpsM"
                     /locus_tag="P5243_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRS18633.1"
                     /translation="MARINGIDLPKNKRGEIALTYIYGIGKSSANKILSKAGVDKDIK
                     VKDWNDDQTTAIRNVISSNYKVEGELRSEVQLNIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGKKKTVANKKKATK"
     gene            complement(2937..3053)
                     /gene="ykgO"
                     /locus_tag="P5243_03950"
     CDS             complement(2937..3053)
                     /gene="ykgO"
                     /locus_tag="P5243_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRS18634.1"
                     /translation="MKVRASVKKRSSECKIVRRKGRIYVINKKNPKFKQRQG"
     gene            complement(3065..3283)
                     /gene="infA"
                     /locus_tag="P5243_03955"
     CDS             complement(3065..3283)
                     /gene="infA"
                     /locus_tag="P5243_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRS18635.1"
                     /translation="MAKQQSIEQDGTIIEALSNAMFRVELTNGHVITAHISGKMRMHY
                     IKILPGDKVKVEMSLYDLTKGRITFRYK"
BASE COUNT         1034 a          604 c          445 g         1366 t
ORIGIN      
        1 gtattaacta ctttttctga agtggaagta gatttttttc gacttcttct taccggagat
       61 ttttttgtac tagtagtttt accttctttc agataagtat cgttaaaatc tactaattca
      121 atataacaca tttcagcagc atcacctaat ctattgccta gttttataat acgagtataa
      181 cctcccggac gttcagcaat ttttgatgat atttctctaa ataattctgt tacagcaaat
      241 ttattctgca agtatttaaa aacaatacga cgtgaatgtg tagtatcctc tttagattta
      301 gtaattaaag gatcaacata tttttgtaat gctttagctt ttgcaagagt agtttttata
      361 cgtttatgca taattaaaga tgttgccata tttgataaca tagcagctct atgttcgtgt
      421 gttctaccta aatggttaac tttctttccg tgtctcattg ttttctataa ttattcttta
      481 tctaatttat atttacttat gtccattcca aaagttagat ttaaagcttc taataattca
      541 tctaattcag taagagattt tttaccaaaa tttctgaatt tcaacaaatc atttttattg
      601 aattgaacaa gttctccgag cgtttcaaca tctgcagctt ttaaacaatt taaagctctt
      661 acagataaat ccatatcaac aagttttgat ttaagaagtt gacgcatatg taatacttcc
      721 tcatcaaatt cttcatttcc aaatttatct tctgtatcaa gcgttatttt ttcatctgag
      781 aacaacataa aatgttgaat caagatttta gcagcgtctt ttaaagcgtc ttttggatga
      841 attgagccgt cacttttaat ttcaagaatt aacttctcaa aatccgtttt ttgctctaca
      901 cgataatttt caacagaata tttcacattt ttaataggtg tgaatataga atctattgga
      961 attattccaa attcgctatc cataatttta ttttcttcag caggaacata tcctcttccc
     1021 ttaccaatcc aaaaatcaat tgtaagtttt actgaggtat tcatattgca aataactaaa
     1081 tcagtattta aaacttgaaa gttagttaaa aatgcattta aatcacctgc aacaaattta
     1141 tctttaccag caacggtaac agatactttt tcagattgaa cagaatcagt tgttactctt
     1201 ttaaatctaa tttgtttaat atttaaaatt atttcagtaa catcttcaac tacacctttt
     1261 atggtactaa actcatgatc tacaccttca attttgatgt tagttatagc atatccttct
     1321 agagacgata ataatactct acgaatagcg ttacctactg taattccata tcctggttct
     1381 aatggacgga attcaaaaat tccaatgtta tcatccgatt caagcattat aactttatcg
     1441 ggtttttgaa atgctaaaat tgccatagac aaaagtcttt ctataaaatt atttagaata
     1501 aagttctact attaattgtt ctttgatatt ttccggtatc tctgatcttt cgggtacatt
     1561 taaaaattta ccttgcattt gagatttatc gaattctaac catgaaaatt tactaacaga
     1621 attagtgctc aatgaacgag ttattgcttc gagtgatctt gatttttcac gaacagcaac
     1681 aatatcacca ggttttacat gaaaagaagg tatattaaca acagctccat ttacggtaat
     1741 atgttgatga gttactaatt gacgagcagc tctgcgtgta tttgcaattc ccattctata
     1801 cacaacgtta tctagacgtt gttctaataa ttgcactaaa atttcacctg taactccagg
     1861 tcttctttca gcctcatgaa ataagttagc aaattgacgt tctaaaacgc catacatata
     1921 tttagctttt tgtttttctt gtaattgaac tccatacacg gatttttttg cttttctttt
     1981 agaaagtcca tgttggccag ggggataatt ctttttttgt aatgcactat ctggtccata
     2041 aattggttct ccaaatttac gtgctatttt tgatgtaggt ccagtatatc ttgccatgta
     2101 actaaatttt tttaaaatta aactcttctt ctttttggag gtctgcaacc attatgaggt
     2161 agcggagtta catcaacaat ttctgtaact tcaattccgg aattatgtat tgttctaatt
     2221 gcagattctc taccaactcc aggtccttta acaaaaactt ttactctgcg taatcctaaa
     2281 tcaaatgctt cttttgcaca atctgttgcg gccatttgag ctgcatacgg agtatttttt
     2341 ttggaacctc taaacccctt tttaccagaa gaagaccatg atattacttg tccttcggca
     2401 tttgtaatcg taacaataat gttattaaat gaagcatgta tatgagcttg tcctgctgct
     2461 tcaactttta ctactttttt tactgacttc gtttttttag ccataatatc aaactataaa
     2521 ttatttagtt gcttttttct tatttgcaac agtttttttc tttccttttc ttgtacgtgc
     2581 attatttttt gtagattgac ctcttaccgg taaccctaaa cgatgacgaa tacctctata
     2641 acaaccaata tccattaatc gtttaatatt taattgaact tcagaacgta attcaccttc
     2701 tactttatag ttagatgaaa taacatttct aattgcagtt gtttgatcat cattccaatc
     2761 ttttactttg atgtctttat caactcctgc ttttgacaaa attttgttag cgcttgattt
     2821 tcctatacca tatatatagg ttaatgctat ctcgcctcgt ttattctttg gtaaatctat
     2881 accgtttatc cttgccatag taacttactt tgaaaattgg tgcaaaatta taaaaattat
     2941 ccttgtcttt gtttaaattt tggatttttt ttgttaataa cataaatcct tccttttctg
     3001 cgaactattt tacattcgct acttcgtttt tttactgacg ctcgaacttt catgtttatt
     3061 tattttattt atatcgaaaa gttattcttc ctttagttaa atcatataat gacatttcta
     3121 cttttacttt atctccaggt aatattttaa tataatgcat tctcatttta ccggaaatat
     3181 gagctgtaat aacatgtcca tttgtaagtt caactcgaaa cattgcatta gacaatgctt
     3241 cgattatagt tccatcttgt tcaatagatt gctgctttgc cataatttaa attgatgatg
     3301 ttgttaatcc ggctgatgat cttcctttta ttctgcctgt tttcattaaa ccatcataat
     3361 gtctatttaa caagtgactt tcaatctgtt gtaatgtgtc tagtataact ccaacaataa
     3421 tcagaagtga agtaccacca taaaaatga
//
LOCUS       DAOTOW010000166         3442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21642_length_3442_cov_3.093003, whole genome shotgun sequence.
ACCESSION   DAOTOW010000166 DAOTOW010000000
VERSION     DAOTOW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21642_length_3442_cov_3.093003"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="P5243_03960"
     CDS             <1..1076
                     /locus_tag="P5243_03960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS18636.1"
                     /translation="IQIIYYLFVYAQVAFYKQKNNQSQSYPPVSIIICAKNEEENLRR
                     FLPQVLEQQYPEFEVVIVNDCSEDDTESYITNLQKKYSNIKYTFIKDDPKFKHGKKLA
                     VTIGIKAARYNHFVFIDADCYPETKSWLSSICTQFNEKTQIVLGYGGYEPQQGFLDKL
                     VRYDTFFISVNYLSFARIGLPYMGVGRNMAYSRAAYEQSSKFTKHYHILSGDDDLFIC
                     EVGRKNNTQIELSGMGFTRSAQVNTFSEWCSQKKRHLTTAGSYRFIHQLLLTLEPFSR
                     IVMYVAIILYALLLGTTYVYVLISLVAVRFLLFFIIHNLNMNRLRESKLLVYSPIFDV
                     LLPFVLTILMIQNKLNTRKRRWK"
     gene            1067..1660
                     /locus_tag="P5243_03965"
     CDS             1067..1660
                     /locus_tag="P5243_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS18637.1"
                     /translation="MEINENFSSKAKQDIVLVEQALQGSQKAYAELLDRYRDTIYFML
                     LKMVKNKNDAEDLTIEAFGKAFKNLEQYTSDFAFSTWLFRIASNNCIDFLRKRKAVTI
                     SIDDNYEKDDYEYSFNIESSELNPEENFIKSQNSVQLAEIVKTLKPRYRELIELRYFK
                     EYAYEEIAQQMNLPLGTVKAQLFRARELLFNVLQHTH"
     gene            1664..2293
                     /gene="rsmG"
                     /locus_tag="P5243_03970"
     CDS             1664..2293
                     /gene="rsmG"
                     /locus_tag="P5243_03970"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRS18638.1"
                     /translation="MNRILEYFPDITKHQQEQFAQLDSLYRYWNERVNVISRKDIDNL
                     YSHHVLHSLAIAKFFDFKQRSRILDVGTGGGFPGIPLAIMFPKVDFVLIDSIAKKISV
                     VDAIAKELQLTNVTAKQMRVEQCRDRFDYIVSRAVTAFPEFYKMSKHAVSTVVNNKLH
                     NGIIYLKGGEFYEEMKGFKKFQIYKISDVYTDDFFETKKIIYVPITPFS"
     gene            complement(2352..>3442)
                     /locus_tag="P5243_03975"
     CDS             complement(2352..>3442)
                     /locus_tag="P5243_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18639.1"
                     /translation="NVENSNIVITASGTLPLEGKIQIQNGNLLLYTGGLTINISDEGG
                     LYLIDSDNNGDGIIDLNWGGITLNNNGEAYVEGLKASGGGTTINVNDGANMFIGNISL
                     YTGLNNNSIHVYNGGNLFYCGNKTPVNEQLGTIHTGGTLNFALGYYTTETPGNQGDFS
                     VSGNETAAFENAEVCRASFYGGTPFGEELLPIELTMLHATCSNGTIHIAWQTASEQNN
                     DFFTVYRSFDGIVFTAIATVSGAGNSNSLLNYMYDDNTLAQGIVYYKLAQTDYDGTTV
                     YSKILAVSTCGNPAIYSFKEGEIDITFENKDIPNHVIITSIDGKILFSKVFIHVESAS
                     IATSFASGVYIVSTIGNKTIISEKFVVN"
BASE COUNT         1136 a          530 c          612 g         1164 t
ORIGIN      
        1 gtattcaaat tatatactat ctttttgtat atgcacaagt agctttttat aaacaaaaaa
       61 ataatcaatc tcaatcatat cctccggttt caataatcat atgtgctaaa aacgaagaag
      121 aaaatttaag acgattttta cctcaagtgc ttgaacagca gtatcctgaa tttgaagttg
      181 taatagtgaa tgattgttcc gaagatgata cggaatcgta tatcacaaat ttgcagaaaa
      241 agtattcaaa tattaaatat acgtttatta aagacgatcc taaatttaaa catgggaaaa
      301 aattagcagt tactattggt ataaaggctg cacgctataa tcattttgtg tttatagatg
      361 ccgattgtta tcctgaaact aaatcatggc ttagttctat atgtacgcag tttaatgaaa
      421 aaacacagat agtgctaggc tatggtggat atgaaccaca gcaagggttt cttgacaaat
      481 tagttcggta tgataccttt ttcatatcgg ttaattattt gtcgtttgct cgaataggtc
      541 ttccatatat gggcgtggga agaaatatgg catattcgcg agctgcgtat gaacaaagta
      601 gtaaatttac taaacattat catatactgt cgggtgacga tgacttattt atttgtgagg
      661 ttggcagaaa aaataataca caaattgagt tgtcgggaat gggttttacg cgatcagctc
      721 aggtaaatac gttttcagaa tggtgttcac agaaaaaacg acatcttaca actgcaggtt
      781 cgtatagatt tattcatcaa ttgttattga cattggaacc gtttagtaga atagtaatgt
      841 atgttgcaat tattttgtat gctttattat tgggtactac atatgtgtat gtgcttatat
      901 cattggttgc ggtgaggttt ttattgtttt ttataattca caatttaaac atgaatcgtt
      961 tgcgagaatc aaaattatta gtatattcgc caatatttga tgtgttgtta ccatttgtat
     1021 taacaatact gatgattcaa aataagctaa atacacgcaa acgaagatgg aaataaatga
     1081 gaatttctcg agtaaggcta aacaagatat agttcttgta gagcaagcat tacaaggaag
     1141 tcaaaaagcg tatgccgaac ttttggatag atatagagac accatttatt ttatgttgct
     1201 taaaatggtg aaaaataaaa atgatgctga agatttaaca atagaagcat ttggtaaagc
     1261 ttttaaaaat ttagaacaat atacttctga ttttgcattt agcacgtggt tgtttcgtat
     1321 agcttcaaat aattgtattg attttttgcg taaacgtaaa gcggtaacaa tttcaattga
     1381 tgataattac gaaaaagatg attacgaata ttctttcaat attgaaagtt cagaattaaa
     1441 tcctgaagaa aattttataa aatcgcagaa ttcagttcaa ttagcagaaa ttgttaaaac
     1501 attgaaacct cgatatcgtg agcttataga attacgctat tttaaagaat atgcctacga
     1561 agaaattgca caacaaatga atttaccctt aggaacagtt aaagcacaat tgtttcgcgc
     1621 tcgtgagttg ttgttcaatg tgttgcaaca tacacattaa gtcatgaatc gtatactcga
     1681 atatttcccc gacattacta aacaccaaca agaacagttt gcacaactcg attccttgta
     1741 tcgttactgg aacgaacgtg taaatgtgat ttcacgaaaa gatattgata atttgtattc
     1801 gcatcatgtt ttgcattcat tggcaattgc aaaatttttc gattttaaac aacgaagtcg
     1861 tatacttgat gttggtaccg gaggcggttt cccggggata cctttagcta ttatgtttcc
     1921 caaagttgat tttgtattga tagattctat tgcaaaaaaa atatcggtag tagatgctat
     1981 cgccaaagaa ttacaactta ccaatgtaac tgccaagcaa atgcgtgtag aacagtgtcg
     2041 cgacagattt gattatatcg taagtagagc agttactgca ttccctgaat tttacaaaat
     2101 gagcaaacat gcggtaagta ctgttgtaaa taataaactt cataatggta ttatatattt
     2161 aaagggagga gaattttacg aagaaatgaa aggattcaaa aaatttcaga tttataagat
     2221 atccgatgtg tatactgatg atttttttga gacaaaaaaa attatatacg tacctattac
     2281 gcctttttct tagaaacttt tttagattgt aaagtaatca aaatataaaa caggttgtca
     2341 aatcacgtag attagttcac tacaaatttt tcacttatta tcgttttgtt gccaattgtt
     2401 gaaacgatat atactcccga tgcaaatgaa gttgcaatgc ttgcagattc tacatgtata
     2461 aatactttcg aaaacaaaat tttaccatca atagaggtaa ttataacatg attgggaata
     2521 tctttatttt cgaatgtgat atcaatttca ccttctttga atgaataaat agcaggatta
     2581 ccgcaggttg aaacggctaa tattttcgaa tatacagttg ttccatcgta atcggtttga
     2641 gcaagtttgt agtaaacaat accttgtgca agcgtgttgt cgtcgtacat gtagttgagt
     2701 aaagaattgc tgttgcccgc accacttact gttgctattg cagtaaatac aataccatca
     2761 aacgaacggt atactgtgaa aaagtcattg ttttgttccg atgcagtttg ccatgctatg
     2821 tgtattgtgc catttgaaca cgtagcatga agcatggtga gttcgatggg gaggagttct
     2881 tcgccaaatg gagtccctcc ataaaaagaa gcgcggcata cttctgcatt ttcaaatgca
     2941 gcggtttcgt ttcctgatac agaaaaatca ccttgattcc caggtgtctc agttgtgtaa
     3001 tatcctaatg cgaagtttaa tgtgcctcct gtgtgaattg tacctaattg ttcgtttaca
     3061 ggagttttgt ttccacaata aaaaaggttt cctccattat atacatggat gctgttgttg
     3121 ttgaggccag tatataaact aatatttcca ataaacatat ttgctccgtc atttacatta
     3181 attgttgtgc cgccaccact tgctttgaga ccttcaacat acgcttctcc attgttgttt
     3241 aatgttatgc ccccccagtt taaatctatt attccatcac cgttattgtc agaatctata
     3301 agataaagcc caccttcatc agaaatattt attgttaatc ccccggtata tagtagtaaa
     3361 tttccatttt gaatttgaat tttcccttct aacggtaatg tgccacttgc agtaattaca
     3421 atatttgagt tttctacatt aa
//
LOCUS       DAOTOW010000167         3441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21648_length_3441_cov_2.739220, whole genome shotgun sequence.
ACCESSION   DAOTOW010000167 DAOTOW010000000
VERSION     DAOTOW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21648_length_3441_cov_2.739220"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="P5243_03980"
     CDS             <1..583
                     /locus_tag="P5243_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRS18640.1"
                     /translation="FDPSKGKFTNYNKLDGIQGNSFNERAAFKSSLGMMFFGGPNGVT
                     YFMPDSITNDTYTGRVVITDLKIYNTSVRIGEEFHGKVILDKSITYSSEIELSYKHNF
                     SIEFAILNYIAPSKNKYKYRLIGFDEDWNFTDANKRFATYTNLSGKEYIFEVTATNND
                     GVWSDQVTRLKIKIIPPFWRTTWFYSILVIVIG"
     gene            547..1521
                     /locus_tag="P5243_03985"
     CDS             547..1521
                     /locus_tag="P5243_03985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS18641.1"
                     /translation="MVLFHISNSNRLIIYIYIKVRERQLIDEKKRLERMVEERTQEIN
                     QQKEELQLQSELLVRNNEELSRTNRLIKDSISYAKRIQDAMLPSMHVLEKHLPESFVL
                     FKPRDIVSGDFYWFAEQGEIIYVAVSDCTGHGVPGAFMSMIGSTLLNEITSEKLHQKP
                     SEILDKLNKGVVTALNQNLDGNADSQDDGMDITLCAINKKEKTIQIACANHIVFRVYN
                     DSIEAIQGDICSIGGLFSAEDNKGYSNFEFKYKSGTRFYLFSDGYQDQFGGKSNKKFQ
                     ASRFKELLFETRNVSMQEQFEILNTTFEEWKGANRQIDDVLVMGIKLD"
     gene            1523..>3441
                     /locus_tag="P5243_03990"
     CDS             1523..>3441
                     /locus_tag="P5243_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRS18642.1"
                     /translation="MNLFVSRLVRVYICFAFVVCSITVNAQHPIVPKSDVVYTLPQIP
                     IDTLQRIQTLFPNMFAYPFFVDINFISQSSSIVVNDTVYYTMHFASPGAYSLNIIFDS
                     LYINEHGIIWLYNPSKTSVAGPFYKKNIHNHSFASPLIWGDTICITYCEPLTVNQNTR
                     IFIRQISHDFKQDFEAKKQLKALADTCNVNINCGEGTTWYNEKRAVCKLIIGGTSICT
                     GTLVNNTSHDATPYVLTANHCLSSQASAEKTVFYFNYEYQDCEGSGQLNNSYVISGSN
                     LVATSPGGKIDFTLLQMVEIPPAAFNVYYAGWDASGTITKGSVCIHHPRGDAKKISID
                     NDSYSTASFLTYVKLSHWKISRWDKGTTESGSSGSALFSMNKQVIGTLSGGEANCSKP
                     INDYFSKLSVAWDYHTNDNERLKPWLDPQNTGKTVCTGFDPNLHDTHVISNIKLTDSI
                     GLYDFANKATGLWTGVNEIGWSQFADYIPFSFNKNIYAISLLGSIDTTQDMSHIIFKI
                     WQGVNKPEQVLYSTPLHKSMVVDSVWIKIIPDEAIQTNGSFWVGYEIQDKCSAFTAFM
                     AKPRSVENSLYIEHPKRWIPANLVGINSSLAVELYVTNQPDTISVIEIEKPIFAAKIN
                     HTIKSYSTVELFAVDS"
BASE COUNT         1200 a          474 c          606 g         1161 t
ORIGIN      
        1 atttgatccg tctaagggta aatttactaa ctataataaa ttagatggaa ttcaaggtaa
       61 ttcttttaat gaacgtgccg cgtttaagtc atcgttggga atgatgtttt ttggaggacc
      121 aaatggtgta acgtatttta tgcccgatag tattacaaat gatacatata ccggaagggt
      181 agttattact gacctcaaaa tttataatac ttcggtgcga ataggtgaag aatttcatgg
      241 aaaagtaatt cttgataaat ctattactta ttcttcagaa atagaattgt catataaaca
      301 taatttttct attgaatttg caattttaaa ttatatagct ccttcaaaaa ataagtacaa
      361 atacagactt attggttttg atgaagattg gaactttacc gatgcgaata aacgatttgc
      421 tacatatact aatttatcgg gtaaagaata tatttttgaa gttactgcaa ctaataatga
      481 cggagtttgg agtgatcagg taacacgatt gaaaataaaa attattcctc ctttttggcg
      541 tacaacgtgg ttttattcca tattagtaat agtaataggt taataattta tatttatata
      601 aaagttcgtg aacgacaact tattgatgag aaaaagcgtt tggaacgaat ggttgaagaa
      661 cgaactcaag aaataaatca gcaaaaagag gaattacaat tacaaagtga attgttggtt
      721 cgtaataacg aagaactttc tcgaaccaat cgattaataa aagatagtat aagttatgct
      781 aagcgtattc aagatgcaat gttaccttct atgcatgtat tagaaaagca tttacccgaa
      841 tcgtttgtgt tatttaagcc tcgtgatatt gtaagtggtg atttctattg gtttgccgaa
      901 caaggggaaa taatatatgt tgctgtttct gattgtacag gacatggtgt gcccggagct
      961 tttatgtcta tgattggttc tacactgctt aatgaaatta cttcagaaaa attacatcaa
     1021 aaaccttcag aaatactcga taaattaaac aaaggtgttg ttacggctct taatcaaaat
     1081 ttagacggaa atgccgattc tcaagatgat ggaatggata taacactgtg tgctattaat
     1141 aaaaaagaaa aaactattca aatagcttgt gctaatcata ttgtgtttag agtgtataat
     1201 gattctattg aggcaattca aggtgatatt tgttctattg gaggtttgtt ttctgccgaa
     1261 gacaataaag gatattcaaa ttttgagttt aaatataaat cgggtactcg tttttattta
     1321 ttttctgatg gataccaaga tcaatttgga ggtaaatcaa ataaaaaatt ccaagcatca
     1381 agatttaaag aattattatt tgaaacgcga aatgtaagca tgcaagagca attcgaaata
     1441 ttgaatacta catttgaaga gtggaagggt gctaatcgtc aaatagatga cgtattagta
     1501 atgggtatta aattagatta atatgaattt gtttgtatct cggcttgttc gagtatatat
     1561 atgttttgct tttgtagtat gttctattac tgttaatgct cagcatccaa ttgttcctaa
     1621 atctgatgta gtttatacat tacctcaaat tcctattgat acattacaaa gaatacaaac
     1681 attgtttcct aacatgtttg catacccatt ttttgttgat atcaatttta tttctcaaag
     1741 ttctagtata gttgtaaatg ataccgtgta ctatacaatg cattttgcat caccgggagc
     1801 ctattctctt aatattatat ttgattcttt atatatcaat gaacatggaa ttatatggtt
     1861 atataatcca tctaaaacat ctgttgcagg tccgttttac aaaaaaaata tacataatca
     1921 ttcgtttgca tcacccctta tatggggaga tactatttgt ataacatatt gtgaaccgct
     1981 tacggtaaat caaaatacta gaatttttat ccgacaaatt tcgcatgatt ttaaacaaga
     2041 ctttgaagca aaaaaacaat taaaagcatt ggcagatact tgtaatgtaa atattaattg
     2101 tggagaggga actacatggt acaatgaaaa aagagctgtt tgtaaattaa ttataggtgg
     2161 tacaagtatt tgcacaggta cattagtaaa taatacatct catgatgcta ctccatatgt
     2221 tttaactgct aatcattgtt tgagttcgca agcaagtgcc gaaaaaacag ttttttattt
     2281 taattatgaa tatcaagatt gcgaaggttc cggacaatta aataattcat atgttattag
     2341 cggttcaaat ttagttgcta catcgccagg tggtaaaatt gattttacac tattgcaaat
     2401 ggttgaaatt ccacccgcag catttaatgt atactatgca ggttgggatg cttcaggaac
     2461 tattacaaaa gggagtgtct gtattcatca tcctcgagga gatgcaaaaa aaatttcaat
     2521 tgataatgat tcatattcaa cagcatcgtt tttaacatat gttaagttat cacattggaa
     2581 aataagtaga tgggataaag gaactacaga atcagggtca tcaggttctg ctttgttttc
     2641 tatgaataaa caagtaattg gaactctttc ggggggtgag gcaaattgtt caaaacctat
     2701 taatgactat ttttcaaaac tatctgtagc atgggattat cataccaatg acaatgaacg
     2761 tttgaaacct tggctcgacc ctcaaaatac cggtaaaact gtatgtacag ggtttgatcc
     2821 aaacttgcac gatactcatg ttatttcaaa tataaaacta acagattcta taggattgta
     2881 tgattttgca aataaagcaa ccggattgtg gaccggtgta aatgaaatag gttggtctca
     2941 atttgctgat tatataccat tttcattcaa taaaaatata tatgcgattt ctttattagg
     3001 aagcatagat actacacaag atatgtcgca cattatattt aaaatatggc aaggagtaaa
     3061 taaaccggag caagtactgt atagtactcc attacacaaa tcaatggtag tagactctgt
     3121 atggattaaa ataatccctg atgaagcaat acaaacaaat ggtagttttt gggttgggta
     3181 tgaaatacaa gataaatgtt cggcatttac agcttttatg gcaaaacctc gttctgtaga
     3241 aaattcttta tatatagaac atcctaaacg gtggattccg gctaacttgg ttggaattaa
     3301 ttcatcattg gctgttgaat tgtatgtaac taatcaacct gatactatta gtgtaataga
     3361 gatagaaaaa ccaatatttg ccgctaaaat caatcatact attaaatcat atagtacagt
     3421 agaattattt gcagtagatt c
//
LOCUS       DAOTOW010000168         3435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21693_length_3435_cov_2.741124, whole genome shotgun sequence.
ACCESSION   DAOTOW010000168 DAOTOW010000000
VERSION     DAOTOW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21693_length_3435_cov_2.741124"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="P5243_03995"
     CDS             <1..273
                     /locus_tag="P5243_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRS18643.1"
                     /translation="DFSELVKGGAVILDVRTKGEYASGHIKGSINIPVEQLAGNLNKL
                     KDKNNVIITCCASGMRSASAKGLLTSRGYVNVYNGGSWSSLERKIN"
     gene            282..1820
                     /locus_tag="P5243_04000"
     CDS             282..1820
                     /locus_tag="P5243_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRS18644.1"
                     /translation="MSKVIFEEEKSFLRTLATGIQLLASIIDKTKQAGKTEIEGNDAF
                     VLYDTYGFPYDLTTLIAKENNLSVDEQGFTVELEKQKERARQATNISTGDWIELQQGN
                     GNSFIGYDTTQATVSILKYRKVHVKGKDLYHIVFDQTPFYAESGGQVGDSGYIEAHGE
                     KVYVLDTQKENNLIIHITETIPADCDIEFHAVVNEKNRVLTQNNHSATHLLHLALQQV
                     LGTHVEQKGSLVNPERLRFDFAHFQKMTDEEIRKVEILVNEAIRSNIQLQEYRETPIE
                     VAKNMGAKALFGEKYGDTVRVIQFGNSVELCGGTHTHATGTIGFFKIISEGAIAAGIR
                     RIEAVTADKAQAYVFETIDIVSGIKEMLKNPAQVLQHVESLINENVQLKKQIEHFEKQ
                     QALAQASQLVNELQLKGNVNVIEVSNAGPLKDIVFAVKEKLDSFVIVLAANIDGKAHI
                     AASVSKDIIESKHITAQEIIKAASSHIQGGGGGQPNFATAGGKNPAGISIALQEVSKI
                     IKLL"
     gene            1866..2444
                     /locus_tag="P5243_04005"
     CDS             1866..2444
                     /locus_tag="P5243_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRS18645.1"
                     /translation="MKHIRTVVIVCCFSMLFSACKSTKQAQALRDCNYSFASISNLKV
                     GDVYIQGKQSFRDLSMQETTGLSQLLMARKLPVSLTAHIAIQNPNSKTAAIDALEWIL
                     EVRNKEVAKGEVTKRVEVKPGQTIQVPIDVATDLGNIFKAFSMKEITDVMFNISNADG
                     IPIEAKLKIKPSITVGRKQIKAPSYFVIDVMK"
     gene            2554..>3435
                     /locus_tag="P5243_04010"
     CDS             2554..>3435
                     /locus_tag="P5243_04010"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRS18646.1"
                     /translation="MNVSVQFFAYFSLFAFFVSLIIGIYIFAKFPRITITRMFFNICL
                     LLAGINYIEYEMRISQNIHEAELWGHLYAIWPFILSTTLHFLLLFRGVIKKPFITHSL
                     LYIPAIVIFYIELTTDYISFKPVQTNWGYTTEPAEGGWFIYSSVWTLSILAAILYRAY
                     QNFLLATGIKKRQAFFIFIGLVINFLIGICTDFLFKIVHVNTPAVGYSYSIISFGLIA
                     FAINRYHLFELTPESLAGKIIATISEMVFLVNSKKQIVEINPAALQVLKYSKEELIGK
                     PIDSIIHNSGELCATCCN"
BASE COUNT         1235 a          465 c          644 g         1091 t
ORIGIN      
        1 gatttttcgg agttagtaaa gggtggtgcg gtaattttag atgtgcgtac caaaggagag
       61 tatgcttcag gtcatatcaa aggttcaata aatatcccgg tggagcaatt ggcaggaaac
      121 ctgaacaaac ttaaagataa gaataatgtt attattacct gttgtgcatc gggaatgaga
      181 agtgcatcag ctaaaggact tttaacttcc aggggatatg taaatgtgta caacggagga
      241 agctggagct cattagaaag gaaaatcaac taataaaatt aatttcaaaa gttattttcg
      301 aagaagaaaa atcattttta cgtactcttg caaccggaat ccaattatta gcttcaatta
      361 ttgataaaac aaaacaagcc ggaaaaacag aaatagaggg taacgatgct tttgttttgt
      421 atgatacata tggattcccg tatgacttga ctacattgat tgcaaaagaa aataatttga
      481 gcgttgacga acaaggattt actgtagaat tagaaaaaca aaaggaacgt gctcgacaag
      541 ctaccaatat ttcaaccggc gactggattg aattgcaaca aggtaatgga aatagtttca
      601 tagggtatga tacaactcaa gctacagtaa gcattttgaa atacagaaaa gtacatgtaa
      661 aaggaaaaga tttatatcat attgtatttg atcaaactcc tttttatgca gaatccggag
      721 gacaagttgg tgatagcgga tatattgaag cacatggaga aaaagtatat gtattagaca
      781 ctcaaaaaga aaataattta attatacata ttactgaaac aattcctgca gattgcgata
      841 tagagtttca tgctgtagta aatgaaaaaa acagagtgtt aactcaaaat aatcattcag
      901 ctacacattt attgcattta gctttacaac aagtattagg aactcatgtt gaacaaaaag
      961 gttcactcgt taatcctgaa cgattacgat ttgattttgc tcattttcaa aaaatgacag
     1021 atgaagaaat acgtaaagtt gagattttag taaatgaagc tattcgttcc aatattcagt
     1081 tacaagagta tcgtgaaact cctatagaag tggcaaaaaa tatgggggct aaggccttat
     1141 ttggcgaaaa atacggtgat acagttcgtg taatacaatt tggtaattcg gtggaattat
     1201 gcggtggcac acatacccat gcaacgggaa caatcggttt tttcaaaatt atatctgaag
     1261 gagctatagc tgcgggaata cgcaggatag aggctgtaac tgccgataaa gcacaggcat
     1321 atgtgtttga aacaattgat attgtttcag gtattaaaga aatgctaaaa aatcctgctc
     1381 aggtattgca gcatgtagag tcattgatta atgaaaatgt acaattaaaa aagcagattg
     1441 aacactttga aaaacaacaa gcattggctc aagcttctca attagtgaat gaattacaat
     1501 taaaagggaa tgttaatgtt attgaagtgt caaatgcagg tccattaaaa gatattgtat
     1561 ttgcagttaa ggaaaaactt gattcttttg taattgtttt agctgcaaat atagatggaa
     1621 aagctcatat agcagcgtct gtatcaaaag atataattga aagtaaacat ataacagctc
     1681 aagaaattat aaaagcagca tcatctcata tacaaggcgg tggtggcgga caacctaatt
     1741 ttgcaacagc aggaggtaaa aatcctgcgg gaatatcaat agcattacaa gaagtttcta
     1801 aaattataaa actgttgtaa aacaattttt gcggagtatt gttagttata caaaaaaata
     1861 atagtatgaa acatataaga acagtagtaa tagtatgttg tttttcaatg ctctttagtg
     1921 cttgtaaatc aactaaacaa gctcaggcac tgcgtgattg taattattcg tttgcgtcaa
     1981 tttctaatct taaagtaggt gatgtatata ttcaaggtaa gcaaagcttc agagatttga
     2041 gtatgcagga aactacgggg ttatcacaat tacttatggc tcgtaaatta ccggtatcgc
     2101 ttacggcaca tattgctatt caaaatccaa attctaaaac agccgctatt gatgcattag
     2161 agtggatttt ggaagtgcgt aacaaggagg tggcaaaagg tgaagtaaca aaacgtgtag
     2221 aagtaaaacc cggacaaact attcaagtgc ctatagatgt tgctaccgat ttagggaata
     2281 tttttaaagc gtttagcatg aaagaaatta ccgatgtaat gtttaatatt tcaaatgcag
     2341 atggtatacc tatagaggcc aaattaaaaa ttaaaccaag tattactgtt gggcgaaaac
     2401 aaataaaagc tccatcatac tttgtaatag atgtaatgaa gtaatctaac ttactgtaaa
     2461 agaaaaaaat attactatta tttattgtta agaaacaaaa taattgtatt ttcgtaagaa
     2521 caatcattaa gcggttttaa cgaaaatata agtatgaatg tatctgtaca attttttgca
     2581 tatttttctt tatttgcgtt ttttgtatcg cttataatag gtatatacat ttttgccaaa
     2641 tttcctcgta ttacaataac tcgcatgttt tttaatattt gtttattgtt ggcaggtatt
     2701 aattatatag aatatgaaat gcgaatatcg caaaatatac acgaggctga attatgggga
     2761 catttatacg ctatttggcc ttttattttg tctactacat tacatttttt gttactgttt
     2821 agaggagtta taaaaaaacc gtttataaca cattctttgc tctatatacc ggcaattgta
     2881 attttttata tagaacttac tacagattat atatcattta aaccggttca aacaaattgg
     2941 gggtatacaa cagaacctgc cgaaggcggt tggtttattt attcatcggt atggacactg
     3001 tctatattag cagcgattct atatagggca tatcaaaatt ttttgttggc aacaggtatt
     3061 aagaagcggc aagctttttt tatatttatt ggattggtaa ttaacttttt aattggtatt
     3121 tgtactgatt ttttattcaa aatagttcat gtaaatacac ctgcagtggg gtattcatat
     3181 agtataatat catttggatt aatagcattt gccattaata gatatcatct ttttgagtta
     3241 actcctgaat ctttggccgg taagattata gcaacaattt ctgaaatggt ttttttggta
     3301 aattctaaaa aacaaattgt tgaaataaac cctgcagctt tgcaagtatt gaagtattct
     3361 aaagaagaat taattggaaa acccattgat tctataatac ataattcagg agaattgtgt
     3421 gctacgtgtt gtaat
//
LOCUS       DAOTOW010000169         3426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21786_length_3426_cov_2.651735, whole genome shotgun sequence.
ACCESSION   DAOTOW010000169 DAOTOW010000000
VERSION     DAOTOW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21786_length_3426_cov_2.651735"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="P5243_04015"
     CDS             complement(<1..227)
                     /locus_tag="P5243_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRS18647.1"
                     /translation="MTVGETIDVQLSVDTDLSPHNVLAYTFAVSYNSNLLQVKNVQYD
                     AKLQNFSNVANTQQAGKVKLSGAATSKIEGNG"
     gene            complement(401..>3426)
                     /locus_tag="P5243_04020"
     CDS             complement(401..>3426)
                     /locus_tag="P5243_04020"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18648.1"
                     /translation="YGGYHGLGGTYTYTSGLDPLTNANTLHNTWRSGVFTFSRTAVNV
                     QNSTFFSCGHGLAYYGPEATGLATNVRIENSDHSPIVQTWSAKPTFNNVEFVNNANQA
                     IYLQDSRIDYNVTLTKTAGIIGSNTPENAVYISSNTHINTGANVQVSPGLIFKGTGFY
                     VDGTLKLNGTAADRITLTSIHDDSKGGDTNNNGNSTTPGKGNWHYDYFGITFRNTTGE
                     NSIKFTDIAYTYHGICFLSSVATVEDCKIEQCSDKGVSIQGTSNAVIRRTAFNNLQVP
                     VHKHAFSTASLHEGNTASNVSIMGIELIGETFNTSGTLPLYTFAGNTDITYWLTQTLT
                     VGSGTTLTIPAGASFKRNLDNYLYNCFDVQGKLNIVGTAEKPVVITDQRDDNYGSPLD
                     FNQDGTVTQNYGRYNHTFINFNSGSSGTLEHLVLKSNGYGVITAGASPTLRNVLFDNL
                     SRGVRMTGIGTAPVIENSVFNNVTFPLETSLLCFPASLTGNTFSGSSYKGIKVMNETL
                     NQNVTVNPLPFGEMTNAPYIFENYVVDAELTINPGVKCKFLDYTGMTVNRWLKAIGTA
                     EKPIVFTSIYDDYYGGDTNADSTASVANGSYWYGIQFADASIDADCQLKHVIVKNAYE
                     AVTTTGASPTLEYVTFYTNRNAVHATGASNPVINNCDFVGMSQRAVNNVNKSFIIQAQ
                     NCWWGSAEGPVVATVPSGTRQAISEGVNVTPIFTSGLNQPLIGDVSTNGTVQAYDASL
                     VLQAAVSAITLNPAQTIAADASGDGSITAYDATLILEYVAGINSNMPGSLKALRYIEP
                     SLTVGSGEITRENDLLLPLVLKGMPSSVGVDMVLTFNPTLLQAIEILPAINTGFMQAT
                     RVDNENGRIYLAAASTDGKAGEDWNMVRFRVSDNVKADFQTNISAELLLVNEKNETSA
                     ATAGTVIFRSPTGFDVGDYDVEVRCFPNPATDVIYLLGVSNEAKVSIFNISGQKIQTV
                     VLSKNKLNISSFSNGLYFMEIEHNGKVQKLKFLKK"
BASE COUNT          976 a          712 c          641 g         1097 t
ORIGIN      
        1 ccgtttcctt ctattttgct tgtagcagct cccgacaatt taactttacc tgcttgctgc
       61 gtgtttgcta catttgagaa gttttgcaat ttggcatcgt attgtacatt ttttacttga
      121 agcaaattag aattatagct tacagcaaaa gtataagcga gcacattgtg cggtgataaa
      181 tccgtatcaa cggacaactg cacatctatc gtttctccta cggtcatttg caaacctgtg
      241 ggtagggaaa aaacagggtt ttgcgccagt cccacaaacg gtatgaaagc aagaagacaa
      301 aatgcaatat aatattttat atgtttcatt tttttctttt tgaagttata gaaataatat
      361 tttttcagaa gcattacgaa cttttatatg cttttactct ctattttttc aaaaacttca
      421 atttttgtac ttttccgttg tgttctattt ccataaaata caaaccatta ctgaaagaac
      481 tgatattcag tttattttta cttaaaacaa ctgtttgaat tttttgtcct gagatattaa
      541 atatacttac tttcgcttcg tttgaaactc ctaacagata tattacatcc gttgcaggat
      601 ttgggaaaca gcgaacttca acatcgtaat ccccgacatc aaaaccagtt ggagaacgga
      661 atattactgt tcctgcagta gcggcagaag tttcattttt ttcgtttaca agtaataatt
      721 ctgctgaaat attggtttga aaatcggctt ttacattatc cgaaacacgg aaacgaacca
      781 tgttccaatc ttctcctgct ttcccgtccg tagaagctgc tgcgaggtaa attcgtccat
      841 tttcattatc aacacgtgta gcctgcataa aacctgtatt gatagccgga agtatttcta
      901 ttgcctgcaa taaagtcggg ttaaaagtaa gtaccatatc cacacctaca gacgaaggca
      961 tacccttcag caccaaagga agtaacaaat cgttttcacg agtaatctca ccggaaccta
     1021 ccgtaagcga aggctctata tagcgaagcg ctttcaaact tcccggcata ttagaattta
     1081 ttccggctac gtattctaat atcaatgtag cgtcataagc cgtaatactg ccatcgccac
     1141 ttgcgtctgc agcaattgtt tgagccggat ttagcgtaat tgccgaaaca gcagcttgta
     1201 aaaccaatga agcatcataa gcttgaactg ttccgttggt acttacatcg ccaatcaaag
     1261 gctgatttaa tcccgatgta aaaatcggag ttacgttaac cccttcacta atcgcttgac
     1321 gtgtacctga aggtactgta gcaacaacag gtccttcggc acttccccac caacaattct
     1381 gtgcctgaat gataaatgat ttattcacat tattgacagc acgttggctc attcctacaa
     1441 agtcacagtt attaattacc ggattagacg cacctgttgc atgcactgca tttctatttg
     1501 tgtagaaagt tacgtattct aacgtcgggc ttgctccggt ggtagtaact gcctcatagg
     1561 catttttcac aatcacatgt ttcaattgac aatcggcatc tatagatgca tcggcaaact
     1621 ggataccata ccaatacgaa ccgttcgcta ccgaagcagt actatcggca ttggtgtcac
     1681 caccatagta atcatcgtaa atagaagtga acacaatcgg tttttctgct gttccaatag
     1741 ctttgagcca acgattgaca gtcattcctg tgtaatctaa aaatttacat ttcactcccg
     1801 gatttattgt cagttctgca tctactacat aattttcaaa aatataaggc gcattggtca
     1861 tttcaccaaa tggaagcgga tttaccgtaa cattttgatt aagtgtttca ttcattactt
     1921 ttattccttt gtaagaagat ccgctaaagg tgtttcctgt caaagaggcg gggaaacaaa
     1981 gcaatgaggt ttcaagcgga aaagttacat tgttaaatac gctgttttct attactggtg
     2041 ctgttcctat accggtcatt cgcactcctc gcgacaaatt gtcaaaaagc acattacgca
     2101 gtgtaggcga agctcctgct gttattacgc catagccatt gcttttcaac accaagtgtt
     2161 ccaatgtacc cgatgaaccg ctgttaaaat tgataaatgt gtggttgtat ctaccgtaat
     2221 tttgggtcac agttccatct tgattaaaat caagcggaga accgtagtta tcatctcgtt
     2281 ggtctgtaat tacaaccggt ttttcggctg ttccgacaat atttaatttt ccctgcacat
     2341 caaaacagtt atacaaataa ttatccagat ttcttttaaa gctcgctccg gcaggtatgg
     2401 ttaatgtagt gccgcttcct actgtcaatg tttgagttaa ccaataggta atatcagtat
     2461 ttccggcaaa agtataaagt ggcaaggtgc ctgaagtatt aaaagtttcg cctatcagct
     2521 caatacccat aatgcttacg ttggaagcgg tattgccttc gtgaagtgaa gctgtgctga
     2581 aagcgtgttt atgtacaggt acttgcagat tattaaatgc tgtacggcgg ataacggcat
     2641 tggaagttcc ctgtatggat actcctttgt cggaacattg ctcaattttg caatcttcta
     2701 cagtagcaac agaactaaga aagcaaatac catgatatgt ataagctata tcggtaaatt
     2761 ttatactatt ttcgcccgtg gtatttctaa aagtaatccc gaaataatca taatgccaat
     2821 tccctttccc gggtgtcgtg gaatttccgt tgttattcgt atcgcctccc ttgctgtcat
     2881 catggataga agtaagtgtt atccgatcgg cagcagttcc gtttagttta agtgtaccat
     2941 ccacataaaa tccggttcct ttgaatatta atccgggact gacttgcaca ttggcacctg
     3001 tgtttatatg tgtgttagac gaaatataca ctgcattttc aggtgtgtta cttcctatga
     3061 ttccggcagt tttggttagt gtaacattgt aatcaattct actgtcttgc aagtatatag
     3121 cctgatttgc attgttaaca aactcaacat tgttaaatgt cggtttagcc gaccaagttt
     3181 gaactatggg ggaatgatca ctattttcaa ttcgtacgtt agtggctaat ccggtagctt
     3241 caggtccgta atatgccaat ccgtgtccac aggaaaagaa tgtactgttt tgaacattta
     3301 cggcagtacg cgaaaacgta aatactcccg aacgccatgt gttgtgcaat gtatttgcat
     3361 tcgtcagcgg atctagtccg gatgtataag tataagtgcc tcctagtcca tgatatccgc
     3421 catagc
//
LOCUS       DAOTOW010000170         3415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21864_length_3415_cov_2.782738, whole genome shotgun sequence.
ACCESSION   DAOTOW010000170 DAOTOW010000000
VERSION     DAOTOW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21864_length_3415_cov_2.782738"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5243_04025"
     CDS             <1..230
                     /locus_tag="P5243_04025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS18649.1"
                     /translation="FSEAIMLMSVGSKYRVFIPQELAYGDRNAGSIEPYSTLIFDIEI
                     IDIPTQTKSETTVQPSSKEVDAKSSETKKKK"
     gene            complement(292..855)
                     /locus_tag="P5243_04030"
     CDS             complement(292..855)
                     /locus_tag="P5243_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRS18650.1"
                     /translation="MKHLFILITVLFSAFQINAQTLAADVSKSKIIWTANKIVGGHTG
                     AIKLQSGNLSLAGNIITGGTFVIDMKSISCSDITDKATNAKLIGHLQSDDFFSVIQYP
                     TATLTITERASFLNNTTVVKGNLTIKGITKPISFTATRNRKTYTAKIPVNRTLYGITY
                     GSGSFFENLGDNAILDEFILDVTLIMK"
     gene            complement(877..1587)
                     /locus_tag="P5243_04035"
     CDS             complement(877..1587)
                     /locus_tag="P5243_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRS18651.1"
                     /translation="MKSILYKSDTIGKANHGWLQTHHYFSFADYYNAERIHFGMLRVL
                     NDDIIAPNNGFGMHPHDNMEIITIPLLGTLWHSDSMGNEGAINHGEIQVMSAGSGVYH
                     SEWNKDIQKPVHLFQIWIYPRSKNITPRYQQINISSVFEQNKLYQIVSPNQHEKGAWI
                     HQNAWIYLGDFNTQSTQTYTLHSHKNGIFVLVIDGKAHIDTFFLYKRDALGIWDTKQI
                     EIHIEAHTQLLLIEVPMN"
     gene            1761..>3415
                     /locus_tag="P5243_04040"
     CDS             1761..>3415
                     /locus_tag="P5243_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS18652.1"
                     /translation="MRLKKIIIIVIACFAAILITALSLQDSTRKEKVVIATIYEILKS
                     FHYEPKNLDDSFSERMYTLYIKSLDYNKMYFTQEEISVLDTYKNQLDNQIQEGSLTFF
                     ELSLELINKQIKKIEEFYPVLLSKPFDYSISETIEIDEKKRDYAKNQEELYGYWAKFL
                     KLQVMDEIIQIENEQKKAREKSDTVILKSFDEIEKIAREKIVKRYNTRFKRLNQTSRN
                     DRFSAYVNALTGGFDPHTNYFPPKDKQDFDISISGKLEGIGATLSERDGYIKVVEIVP
                     GSPSWKQGELKPEDIILKVAQGDNEPVDIVDMRLDEAVQLIRGPKGTKVVLTVKKIDG
                     KIVRIPIIRDVIMLEEKYAKSAILTVENSKNPKVGYIYLPQFYTDMNDANGRRCSEDI
                     KKEIRLLQNENVAGIILDLRDNGGGSLNDVVEIGGYFIEKGPIVQVNSTGNSRRVLSD
                     YNANIEYDGPLVIIVNSFSASASEILAAAMQDYKRAVIVGTKSTYGKGTVQRFVDVDR
                     LIENQYKDLLPLGAVKVTIQKFYRINGGSTQLKGVTPDVFLPD"
BASE COUNT         1266 a          461 c          548 g         1140 t
ORIGIN      
        1 gttttagcga agctattatg cttatgtctg taggatcaaa atatcgagta tttattcctc
       61 aagaattagc atatggagat cgtaacgcag gttctataga gccttattct acacttattt
      121 ttgatattga aattattgat ataccaactc aaacaaaatc agaaacaact gttcaaccaa
      181 gttcaaaaga agtagatgca aaatcaagtg aaacaaaaaa gaaaaaataa tgacaatatt
      241 taacacaaaa aaagcaaccc aaaaggttgc tttttttgtt tgtacactat gttatttcat
      301 aattaatgtt acatctagta taaattcatc gagaattgca ttatcgccta aattttcaaa
      361 aaaacttcct gaaccatagg ttataccgta aagcgttcta ttaacaggaa ttttagcagt
      421 atacgttttt ctatttcgag ttgcagtaaa tgaaattggt tttgtaatac ctttaatagt
      481 taaatttcct ttgactacag ttgtattgtt aagaaatgat gcacgctcgg taatagttaa
      541 cgtagcagta ggatattgaa taacactaaa aaaatcatcg ctttgtaaat gaccaattaa
      601 tttagcattt gtagctttat ctgtaatatc gctgcacgaa attgatttca tatcaatgac
      661 aaaagttcca ccggtaatta tattacctgc tagtgataaa tttcctgact gcaattttat
      721 tgcacctgtg tggccgccta caattttatt agcagtccaa ataatttttg atttactaac
      781 atctgctgca agtgtttgag catttatttg aaatgctgaa aacaaaactg taattaatat
      841 aaaaaggtgt ttcataatag tataatttta aagtgattaa ttcataggta cttcaataag
      901 taataattgt gtatgtgcct caatatggat ttcaatttgt tttgtatccc aaattcctaa
      961 agcatcgcgt ttatacaaaa aaaaagtatc aatatgggct ttaccatcta taactaaaac
     1021 aaaaataccg tttttgtgag aatgaagcgt atatgtttga gtagattgag tattaaaatc
     1081 gcctaaataa atccatgcat tttgatgtat ccatgctcct ttttcatgct gatttggtga
     1141 tacgatttga tataatttgt tttgttcaaa tactgaagaa atattaattt gttgatatcg
     1201 aggagtaata tttttactgc ggggatatat ccatatttga aataaatgaa caggtttttg
     1261 aatatcttta ttccattcac tgtgatatac tccggaacct gcactcatta cttgtatttc
     1321 tccatgattt attgctcctt catttcccat actatcacta tgccataatg tacccaataa
     1381 gggtattgtt ataatttcca tattatcatg agggtgcatg ccaaatccgt tattgggagc
     1441 tattatatca tcatttaaaa ctcttagcat tccaaaatga attcgttcgg cattataata
     1501 atcggcaaaa ctaaaataat gatgcgtttg taaccaccca tgattagctt ttcctattgt
     1561 atctgatttg tataatatag atttcatgta aatttttatt gtattataat tgtttcatat
     1621 gttttgtcaa cgtttacagt aaaaacttac atagaagaat tctattaaga tttaataatc
     1681 gttttaaaat aaattaatca ttccaacgtg catacgatat tattttgtaa tttcgttttt
     1741 tcatttataa ttgtaacagt atgagattaa aaaaaataat aataatcgta attgcttgtt
     1801 ttgcagcgat tttaatcacg gctctttcac tacaagattc tacacgtaaa gaaaaggttg
     1861 taattgcaac tatttatgaa atattaaaat catttcatta tgaacctaag aatttagatg
     1921 attctttttc ggaacgtatg tatacactgt atattaaaag cttagattat aacaagatgt
     1981 attttacaca agaagaaata agtgttttag atacatacaa aaatcaactt gataatcaaa
     2041 ttcaagaagg cagtttaaca ttttttgaat tatcgttgga attgataaat aagcaaataa
     2101 aaaaaataga agaattttat cctgttttac tttcaaaacc ttttgattat tcaatttctg
     2161 aaacaattga aatagatgaa aaaaaacgtg attatgctaa aaatcaagag gaattatacg
     2221 gatactgggc aaaatttttg aaattacaag taatggatga aataattcaa attgaaaatg
     2281 aacaaaaaaa agcacgtgaa aaaagtgata ctgttatatt aaaatcattt gatgaaatag
     2341 aaaaaatagc acgtgaaaaa attgtaaaac gctataatac aagatttaaa cgacttaatc
     2401 aaacatcaag aaatgatagg tttagtgcat atgtaaatgc attgacaggg ggctttgacc
     2461 cacatacaaa ttattttcca ccaaaagata aacaagattt tgatatttca atttcaggta
     2521 aattagaagg aattggagca acattgtctg aacgtgacgg atatataaaa gtagttgaaa
     2581 ttgttcccgg aagtccatca tggaaacaag gagagttaaa gccagaagat atcattttaa
     2641 aagttgctca aggcgataat gaacctgtag atattgttga tatgcgatta gatgaagcgg
     2701 tacaactaat aagagggcct aaaggaacaa aagtagtgtt aacggttaaa aaaattgacg
     2761 gaaaaattgt tcgtatacca attatacgag atgtaataat gttagaagaa aaatatgcaa
     2821 aatcagcaat tttaactgtt gaaaattcta aaaatcctaa agttggatat atctatttgc
     2881 ctcaatttta tactgatatg aatgatgcaa acggaagacg ttgttctgaa gatataaaaa
     2941 aagaaatacg attattacaa aatgaaaacg tagccggaat tattcttgat ttaagagata
     3001 atgggggagg ttcattaaat gatgtagtag aaataggagg ctattttata gaaaaaggcc
     3061 cgattgtaca ggttaattct accggtaata gcagaagagt gttaagtgat tacaatgcaa
     3121 atattgaata cgatggccct ctggttatta tagtaaattc gtttagcgca tcggcatctg
     3181 aaattcttgc agctgctatg caagattata aaagagctgt aattgttgga accaaatcaa
     3241 cgtatggtaa aggaactgtg cagcgctttg tagatgttga cagacttata gaaaatcaat
     3301 ataaagactt attaccactt ggagcggtaa aagtaacaat tcaaaaattt tatagaatta
     3361 acggtggttc tacacaatta aaaggagtta cccccgacgt atttttacct gatat
//
LOCUS       DAOTOW010000171         3408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21914_length_3408_cov_3.631077, whole genome shotgun sequence.
ACCESSION   DAOTOW010000171 DAOTOW010000000
VERSION     DAOTOW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21914_length_3408_cov_3.631077"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="P5243_04045"
     CDS             complement(<1..742)
                     /locus_tag="P5243_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18653.1"
                     /translation="MKLICVFISFTITLHSIAQNNKDLAYEKATTAISLMDKGMYNES
                     IGILKESQKLDPTNILYSYEIAYAYYCMKEYKKTIKILKPHTNHTDANDQYFQLLGNC
                     YDYIGKPQKALLVYNYGLTKFPKSGKLYLEKGNVYWGQQQYNRALKMYESGIEADPFF
                     PSNYYRAALLYCNSQNPVWGMIYGEIFMNLETKTKRTSEISKLLFKTYTNQISFNKDS
                     ILQVSFCKNFSISENQFEDSSKHIKIPFC"
     gene            911..1711
                     /locus_tag="P5243_04050"
     CDS             911..1711
                     /locus_tag="P5243_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18654.1"
                     /translation="MIDEEHLLEHLQSLFGKNPEQFNIIEEQIDIQLQMSYFKRSKRL
                     KKKKVELQDMLQKVPLLYQDDVRIEEKRDILIHLASFEQVEAFRAIEAFWKVAEGDIK
                     PWASMAYRENKMMLESSLLDEKQILISTGLGGKGQKLRYFLVFVHAQNEDFTETQQKI
                     VRNELEFVLHKQNSEVESVEFCNQLVKIFVLIPISIAIKDLIVHVVAECNQYGNFVSE
                     NFLITNVKELENEEIFQIIQEKSIESEYDDFDEIEGFEDDEDEDDDDE"
     gene            1704..2669
                     /locus_tag="P5243_04055"
     CDS             1704..2669
                     /locus_tag="P5243_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRS18655.1"
                     /translation="MNNHIFEWGHERPYNSYANYIKQEFGGRIQKISLHAGLTCPNRD
                     GSIGVGGCSFCNNDAFITEYCSPHKPISQQITEGKLFVQRRYKKAQKYFAYFQAYSNT
                     YTSIENLRVLFKEVQKFPEIIGFIIGTRPDCIDEQKLDFFAQLRKHYYIVLEYGVESI
                     YDSTLLAINRGHTYKVSKNAIQMTADYGIHVGAHIIVGLPGETQEMIMAEAFELSQLP
                     LHSLKLHQLQIVAGTRFAQEYEQNPHKFQLFSPELYIPFIAEFVSKLSPTIKIERLAG
                     DSPIRVRIAPVWQGVKYESFVQKFEEYMFAHNLWQGKHYVQKNIM"
     gene            complement(2688..3113)
                     /locus_tag="P5243_04060"
     CDS             complement(2688..3113)
                     /locus_tag="P5243_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18656.1"
                     /translation="MKTKILLFVTCFSLVFFNSCSKKEGESEFKNQITLGTGLNYSNY
                     FELTGVSSDFYLVNGSVIIYFKVESKQDIAGNAIMLEFRNLQNEVIHTISRPSTQDYG
                     HMLLSSFEWTQSAGTYLVKAYIVKEDSTDFVAETTITIH"
     gene            3227..>3408
                     /locus_tag="P5243_04065"
     CDS             3227..>3408
                     /locus_tag="P5243_04065"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRS18657.1"
                     /translation="MKHIVVILCLLTVQVAVQAQQQDVIIASFEQGDVEKLSQYFDEN
                     IDLTVLQNEGIYSKAQS"
BASE COUNT         1226 a          428 c          561 g         1193 t
ORIGIN      
        1 agcaaaatgg tatttttata tgttttgaac tatcttcaaa ttgattttca gaaattgaga
       61 agtttttaca aaatgatact tgtagaatac tgtctttatt aaaactgatt tgattagtat
      121 aggttttaaa taataattta cttatttctg atgtgcgttt tgtttttgtt tcaagattca
      181 tgaaaatttc gccataaatc atgccccata ccggattttg agaattacaa tacaacaatg
      241 cggcacgata ataatttgat ggaaaaaacg gatctgcttc aataccactt tcatacattt
      301 ttagagccct attgtattgt tgttgtcccc aatacacatt ccctttttct aaatataatt
      361 tccctgattt tggaaactta gttaatccgt agttatatac taataatgct ttttgtggtt
      421 ttccaatata gtcataacaa ttaccaagta gttgaaaata ttggtcattt gcgtctgtat
      481 gatttgtgtg tggtttcaga attttaattg tttttttgta ttctttcatg caatagtatg
      541 catacgctat ttcataagaa tacaaaatat tagtagggtc aagtttttga gattctttta
      601 aaattccaat actttcattg tacatccctt tatccataag actaattgca gtagttgctt
      661 tttcgtatgc taaatctttg ttgttttgag caatactatg taatgttata gtaaaagaaa
      721 tgaatacaca tattagtttc atgatatcta taaaattctt gttaaacaaa tataaagttt
      781 ttagttaaaa agaatatata taaattacaa ataatcatgc tcatatattt aataaacaaa
      841 aaaagaatag gataataaaa attgcaaaaa gtattatttg gcatatattt gcattgaata
      901 atttgtagat atgatagacg aagaacattt attggaacac ctgcaatcat tgtttggcaa
      961 gaatccggaa caatttaata taattgaaga acaaatagat attcaattgc aaatgagcta
     1021 tttcaaacgg tctaaacgtt tgaaaaagaa aaaggttgaa ttacaagaca tgttacaaaa
     1081 agttccctta ttatatcaag atgatgtgcg aatagaagaa aaacgagata ttctgattca
     1141 tttggcatca tttgaacaag tggaggcttt tagagcaatt gaggcatttt ggaaagttgc
     1201 cgaaggtgat ataaaaccat gggcctctat ggcatataga gaaaataaga tgatgttgga
     1261 aagttcatta cttgatgaaa aacaaatact tatatcaaca ggcttggggg gtaaaggtca
     1321 aaaattgcgt tattttttag tctttgttca tgctcaaaat gaagatttta ctgaaacaca
     1381 acaaaaaatt gtacgaaatg aattggaatt tgtattgcac aaacaaaatt cagaagttga
     1441 atcggtagag ttctgtaatc agttggtcaa aatatttgtt ttaataccta taagcattgc
     1501 tataaaagat ttaatagtac acgttgttgc cgaatgtaat cagtatggca attttgtgtc
     1561 agaaaatttc ttaataacaa atgtgaaaga acttgaaaat gaagaaattt ttcaaattat
     1621 tcaagaaaaa tctattgaat cggaatatga tgattttgat gaaatagaag ggtttgaaga
     1681 tgatgaagat gaagacgatg atgatgaata atcatatctt tgagtggggg catgagagac
     1741 cttataattc atatgcaaat tacataaaac aagaatttgg aggaagaatt caaaaaataa
     1801 gtctacatgc cggattaacg tgtccaaacc gtgatggtag tataggtgtt ggcggttgtt
     1861 ctttttgtaa taatgatgct tttataacag aatattgttc tccacataaa ccaatttctc
     1921 aacaaataac agaaggaaaa ttatttgttc aacgaagata taaaaaagct caaaaatatt
     1981 ttgcttattt tcaagcatat tcaaacacat atactagtat tgaaaattta cgagtgttgt
     2041 ttaaagaagt tcagaagttt cccgaaatta ttggattcat tattggtaca cgtcccgatt
     2101 gtattgacga acaaaaatta gatttctttg ctcaattacg aaaacactat tatatagttc
     2161 ttgaatatgg tgtagaatca atatatgatt ctacgttgct tgcaataaat cgtggtcata
     2221 cttataaagt tagtaaaaat gctatacaaa tgacagcgga ttatggaata catgtaggtg
     2281 ctcatattat tgttggtctt cctggtgaaa cacaagaaat gataatggct gaagcatttg
     2341 aattgtctca attaccgttg cattctctta aattgcatca attgcagata gttgcaggta
     2401 cacggtttgc tcaagaatat gaacagaatc cacacaaatt tcaacttttt agtcctgaat
     2461 tatatattcc ttttatagca gaatttgtaa gtaaattatc accaacaata aaaattgaac
     2521 gcttagcagg cgatagtcct attcgtgtac gtatagctcc tgtgtggcaa ggagtaaaat
     2581 atgaaagttt tgtgcaaaaa tttgaagaat atatgtttgc acataatctt tggcaaggta
     2641 aacactatgt gcaaaaaaat attatgtaga tattgttttt ataatggtta atgaattgtt
     2701 attgttgttt ctgcaacaaa atcagtagag tcttctttta caatatatgc ttttactaaa
     2761 tacgtgcctg cagattgcgt ccattcaaac gatgatagta acatgtggcc gtaatcttga
     2821 gtagaaggac ggctaatagt gtgtattact tcattttgta aatttctaaa ttcaagcatt
     2881 attgcatttc cggcaatatc ttgtttcgat tctaccttga aatatattat tacacttcca
     2941 tttactaaat aaaaatcaga gctaactccg gtaagttcaa aataatttga ataattaagt
     3001 cctgtaccaa gtgttatttg atttttgaat tcagattcac cttctttttt agagcatgaa
     3061 ttaaaaaata caagagaaaa acaagtaaca aataaaagta ttttggtttt catataaact
     3121 attttttaaa ataaatgtaa aaatcatata caaaggtata tagtttgtag taattttttt
     3181 tgaaaaatat ggtaaataat ttatttttac aaaaattatt atgagtatga aacacattgt
     3241 agtgatatta tgtttgttga ccgttcaagt agcggttcaa gctcagcaac aagatgttat
     3301 aattgcatcg tttgaacaag gagatgttga aaaactatcg caatattttg atgaaaatat
     3361 tgatttaacg gttttacaaa atgaaggtat ttatagtaag gctcaatc
//
LOCUS       DAOTOW010000172         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_21987_length_3398_cov_2.768172, whole genome shotgun sequence.
ACCESSION   DAOTOW010000172 DAOTOW010000000
VERSION     DAOTOW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21987_length_3398_cov_2.768172"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="P5243_04070"
     CDS             <1..891
                     /locus_tag="P5243_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS18658.1"
                     /translation="DSLVSRHDVIVYDSESHACIMDGIFLHKAKGGKSFVFPHNDIAK
                     CEKMLGFAKKITDETNGGIMVITEGVFGMAGDLGKLDEIAALKEKFDFRLLVDDAHGF
                     GTMGHDGSGTGTHFGVQDKIDIYFSTFAKAMASIGAFVAAEEKIIDYLMYTMRSQIFA
                     KSLPMPLVIGAMKRLDLIQTRPELREKLWTVVNALQTGFKEAGFDIGKTQSPVTPVFL
                     KGTVAMAGNLALDLRENYGLFCSVVIYPVVPKDVIMLRIIPTAAHTLEDVKYTIESFK
                     AIKHKLDSGMYASDKIQMNI"
     gene            954..1499
                     /locus_tag="P5243_04075"
     CDS             954..1499
                     /locus_tag="P5243_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRS18659.1"
                     /translation="MTHNISDISLNQSQHIQNVGFCEYKHTGLYVDNSFIIPEQLALR
                     TKYFIPAHTVLSIQQCNVSAIRTRTSIQVASELHVEADDCIRFLNHSCQPNAAIISKY
                     SEKDNYATIVLESIRDIAMDDEICFDYASTEFTLTEELLNKPCLCNSPRCRGILTGYS
                     ELSQEQKQYLVAHIPVAHFLL"
     gene            1504..2124
                     /gene="recR"
                     /locus_tag="P5243_04080"
     CDS             1504..2124
                     /gene="recR"
                     /locus_tag="P5243_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRS18660.1"
                     /translation="MNQFEYPSKILEQAVLEIGKLPSIGKKTALRLALHILKQDVEYA
                     QKLGEAIIQLRQNITFCKICNTISDTELCQICANPKRDTSVICVVESVKDVLVLEQTQ
                     QFKGVYHVLGGVISPIDGVSPNDLNITSLIERIGNNSVKEIIFALPVTIEGDTTCFYI
                     YKKIQHYSITVTMLARGVAVGNELEYTDEATLARSLRERIMYTGTK"
     gene            complement(2144..2548)
                     /locus_tag="P5243_04085"
     CDS             complement(2144..2548)
                     /locus_tag="P5243_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18661.1"
                     /translation="MCAIQNSLFAQIDSTIMRNEKMCYSLYANSKEFGVYRDQKVFVD
                     SIQKQNIQFLFTCDMTKLFEVKNQNSWKTFYILSTDESDATTYTYSCLGTDSKKYVFI
                     LDLDTHTLTKMYEDSLTFVELNAIESLRIIEN"
     gene            complement(2580..2861)
                     /locus_tag="P5243_04090"
     CDS             complement(2580..2861)
                     /locus_tag="P5243_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRS18662.1"
                     /translation="MLQVNEYFEGNVKSIGLRNQDGKATVGVIAPGEYEFGTSTKEYM
                     HIVSGTLEALLPEQTEWKKYVRGDMFIVPCCSKFKVRATSDVAYLCIYE"
     gene            complement(2875..3246)
                     /locus_tag="P5243_04095"
     CDS             complement(2875..3246)
                     /locus_tag="P5243_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRS18663.1"
                     /translation="MEIQGNIIQILPLQSGTGRNGEWKKQEFILETTDKFPKKICIAI
                     WGDKIQESILQPGNKVTVQFDIESREYNGRWYTEAKAWKIESTATSTQTPAPVSSQTD
                     IPLPDTSDFLMPEQSGDDLPF"
BASE COUNT         1114 a          490 c          559 g         1235 t
ORIGIN      
        1 gattctttag tatctcgtca tgatgttatt gtgtatgatt ctgaatcaca cgcatgtatt
       61 atggacggaa tatttttaca taaagcaaaa ggaggaaaaa gttttgtatt tcctcataac
      121 gacatagcta aatgtgaaaa aatgcttggt tttgcaaaaa aaataactga cgaaacaaat
      181 ggtggtatta tggttattac cgaaggtgtt tttggtatgg ctggtgacct tggtaaactt
      241 gacgaaattg ccgctttaaa agaaaaattt gatttccgtt tattagttga tgatgctcat
      301 ggttttggta ctatggggca tgatggtagt ggaacaggaa ctcattttgg tgttcaagat
      361 aagattgata tttattttag tacgttcgct aaagctatgg cttcaattgg tgcatttgtg
      421 gcggcagaag agaaaattat agattattta atgtatacta tgcgttctca aattttcgca
      481 aaatcgttac ctatgcctct tgtaatagga gcaatgaaac gcttagactt aatccaaact
      541 cgtcctgaat tacgtgagaa attgtggact gttgtgaatg ctttacaaac aggatttaaa
      601 gaagctggtt ttgatattgg taaaacacaa tctcctgtta cccctgtatt tttaaaaggt
      661 acagtagcta tggcaggaaa tttggctctt gatttacgcg aaaattatgg attgttctgt
      721 tctgttgtta tatatcctgt tgttcctaaa gatgttatta tgcttcgtat tattccaaca
      781 gcagctcata cactcgaaga tgtaaaatat acaattgaat catttaaagc tattaaacat
      841 aaattagata gcggaatgta tgcatctgat aaaattcaaa tgaatattta atatcatttt
      901 tatactatat tagtaaataa gggaattcgt atgaattccc ttttttttaa accatgacac
      961 acaatatttc tgatatatct ttaaatcaaa gtcaacatat tcaaaatgtt ggcttctgtg
     1021 aatataaaca tacaggtttg tatgttgata atagttttat aatccctgaa caactagcat
     1081 tacgtacgaa atattttata cctgcgcata cagtgttaag tattcaacaa tgtaatgtgt
     1141 ctgctatacg tactcgaact tctattcaag ttgcttctga attacacgtt gaagccgatg
     1201 attgtattcg ttttcttaat catagttgcc aaccaaatgc tgcaattatt agcaaatatt
     1261 ctgagaaaga taattatgca actattgtgt tagaatctat tcgtgatatt gctatggacg
     1321 atgaaatatg ttttgattat gcatcaactg aatttaccct aacagaagaa ttattgaata
     1381 agccgtgttt atgtaattct ccacgatgca gaggaatact aacaggttac agtgaattat
     1441 cacaagagca aaaacaatat ttagttgctc atattcctgt tgctcatttt ttactataat
     1501 tctatgaatc aatttgaata tccatcaaaa attttggaac aagctgttct ggaaattggt
     1561 aaattaccaa gtattggaaa aaaaacagct ttacgtttag ctttgcatat tttaaaacaa
     1621 gatgtagaat atgctcaaaa attaggtgaa gcaattattc aattacgaca aaatattaca
     1681 ttttgtaaaa tatgtaatac tatttctgat actgaattat gtcaaatttg tgctaatcca
     1741 aaacgtgata catctgttat atgtgtagta gaatctgtaa aagacgtgtt ggttttagaa
     1801 caaacacaac aatttaaagg ggtgtatcat gtattaggtg gtgtaatatc tcctattgat
     1861 ggagtttctc ctaatgattt gaatattact agtttaattg agcgaatagg aaataattct
     1921 gtaaaagaaa taatattcgc gcttcctgta acaattgaag gtgatactac ttgtttttat
     1981 atttacaaaa aaattcaaca ttattctatt acggtaacta tgcttgccag gggggtagct
     2041 gttggaaatg aattggaata taccgatgaa gctactttgg ctcgttcgtt acgagagcgt
     2101 ataatgtata cagggactaa ataatccggg aaattacaaa gtattaattc tcaataattc
     2161 gtaaactttc aatagcattt aattctacaa atgtaagtga gtcttcatac atttttgtaa
     2221 gtgtatgtgt atctaaatca agtatgaaaa catatttttt actatcggtt cccaaacaac
     2281 tgtatgtata agtagtagca tcactttcat ctgttgatag tatataaaat gttttccaag
     2341 aattttgatt ttttacttca aaaagttttg tcatatcgca ggtaaacaaa aattgaatat
     2401 tttgtttttg tatagaatct acaaatactt tttgatctct gtatacgcca aattctttag
     2461 aatttgcata caaagaataa cacatttttt catttcgcat tattgtactg tcaatttgtg
     2521 caaacaatga attttgaatt gcacacatag atatgaaaat aagaaaaaga aataaatgtt
     2581 tattcatata tgcaaagata agcaacatca gaggtagcac gtactttaaa tttactgcaa
     2641 caaggaacaa taaacatatc tcctcgcaca tattttttcc attcagtttg ttcaggtaat
     2701 aatgcttcta aagtacccga aactatatgc atgtattctt ttgttgatgt tccgaattcg
     2761 tattcaccgg gtgcaattac tccaactgtt gcttttccgt cttgatttcg taaccctatg
     2821 gattttacgt ttccttcaaa atattcattt acttgtaaca ttgtgtatat aattttaaaa
     2881 tggtaaatca tcgccagatt gttcaggcat caaaaaatca gaagtgtcgg gtaggggaat
     2941 atctgtttgt gaagataccg gagctggagt ttgggtagaa gttgcagttg attcaatttt
     3001 ccatgccttt gcttcggtat accatcgtcc attatattct ctactttcaa tatcaaattg
     3061 aacagtaact ttgtttcccg gttgcaatat tgattcttga attttatctc cccaaattgc
     3121 aatacaaatt ttttttggga atttatctgt agtttctaaa ataaattctt gttttttcca
     3181 ctctccgtta cgacctgttc ccgattgtaa tggtaaaatt tgtattatgt taccttgaat
     3241 ttccattgtt tttttattat gcaaacatac aattttaatt gttttataca ataaaatacg
     3301 tggtgaaatc aaacaatttt tttgtacatt cgtacaaacg ttaactattc ttaatgaaaa
     3361 gaaaatgaaa tacttgatat tacttatttg tttttttt
//
LOCUS       DAOTOW010000173         3394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22017_length_3394_cov_4.065289, whole genome shotgun sequence.
ACCESSION   DAOTOW010000173 DAOTOW010000000
VERSION     DAOTOW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22017_length_3394_cov_4.065289"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /gene="secA"
                     /locus_tag="P5243_04100"
     CDS             complement(<1..1638)
                     /gene="secA"
                     /locus_tag="P5243_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843968.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRS18664.1"
                     /translation="MSLVQKILQAFLGNKSQRDIQAILPLVQKIKEFESEYSGLSHDE
                     LRNVTVKLRTIIQEAVKPMQTQIDSLQNSLDNDENLSFAKREEMYDSIDSLKKEIDTT
                     IEKTLEEILPQAFAVVKETAKRFTHNTTVEVTALDYDKELATRKDHITIQGDKAIWKN
                     SWLAGGNMITWDMIHYEVQLIGGTILHQGKIAEMATGEGKTLVATLPVFLNALPGKGV
                     HVVTVNDYLAKRDSEWMGPIFEFHGLRVDCIDNHEPNSEARKKAYQADITYGTNNEFG
                     FDYLRDNMATTPEELVQRIHNFAIVDEVDSVLIDDARTPLIISGPIAKKNNSDELFIE
                     YRPRVEKIYNAQKAYVTRILADAKNMIASEDKKTAEEGAKLLVRAFKGLPKNKALIKF
                     LSEQGMKTLLTKTENFYMAENNKNMHLVTDELYFVIDEKNNSIELTEKGIDFLSSDLE
                     DKNFFVMPDIGERIAEIEKSNISAEEKLQLKDSLTQDYAVKSDRVHTINQLLKAYALF
                     EIDVEYVVIDNKVKIVDEQTGRIMEGRRYSDGLHQAIE"
     gene            complement(1818..2582)
                     /locus_tag="P5243_04105"
     CDS             complement(1818..2582)
                     /locus_tag="P5243_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HRS18665.1"
                     /translation="MNFPLSYIPQREKKPRNAGQTMIIDKGLTSIEAQGLAQDVHEYI
                     DFAKLGFGTSLITHNLEKKIAIYKEAGIIPYFGGTLFELFIIRNQFDEYQRFLDTYGI
                     EVAEVSDGSMNMKHEDKLSYISQLSKHFTVISEVGSKDASVVITPEEWILQMQSELDA
                     GSVKVITEARESGTIGIFNKDGSPNKTLINTIISNIDITKVMWEAPLKAQQALFIQLL
                     GYNVNLGNIATNEVLALEALRLGLRGDTFFQFLPQK"
     gene            complement(2591..>3394)
                     /locus_tag="P5243_04110"
     CDS             complement(2591..>3394)
                     /locus_tag="P5243_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18666.1"
                     /translation="ADPYSEYAWFNLALVYLQSEEIDEAIQAFDYAITINESFSSAHF
                     NKANCYMTKELYNEAILCYQDVLQFEPENIYALQYIAECYEKLGEIEHSMATYEKITE
                     IAPEYGEGWYGIALLHFYNNNTQKALELFEKALELDSQNDKYLISYGIALSDCGMYPK
                     AIQIFKQAIAINKFEFEAWIQYSQIEMELNNPNVAKKILRQGIEAAPHATLYFTLAAY
                     HANEHDIPKAIDYFKKGYSLDPEYATMYFFDICHLPLNSLQLFYNEILK"
BASE COUNT         1006 a          539 c          528 g         1321 t
ORIGIN      
        1 ttctattgct tggtgcaagc catccgaata acgacggcct tccataatac gaccggtttg
       61 ttcatcaaca atttttactt tattatcaat tactacatat tctacatcta tttcaaataa
      121 tgcatatgct ttgagtaatt gattaatagt gtgaaccctg tctgatttta ctgcatagtc
      181 ttgtgtaaga gaatctttca attggagttt ttcttcggca gaaatattcg atttttcaat
      241 ttcagctatt cgttcaccta tatcgggcat tacgaagaaa tttttatctt ccaaatcaga
      301 tgataaaaag tcaattcctt tttcggttaa ttctatagag ttattttttt cgtcaatcac
      361 aaaatacagt tcatctgtta ccaaatgcat gtttttattg ttttcggcca tataaaaatt
      421 ttctgttttt gttaacagag ttttcatgcc ttgttcactt aaaaatttga taagtgcttt
      481 atttttaggt aatcctttga atgctcgtac aagtaattta gctccttctt ctgccgtttt
      541 tttatcttca cttgctatca tatttttggc atcggcaagt attcgagtaa catatgcctt
      601 ttgtgcattg tagatttttt caacccgcgg acgatattct ataaataatt catcgctatt
      661 gttttttttt gcaataggac cagaaataat aagtggtgta cgagcatcat ctattaatac
      721 agagtcaacc tcgtcaacta ttgcaaaatt gtgaatgcgt tgaactaatt cttccggtgt
      781 agtagccata ttgtcacgca agtaatcaaa gccaaattca ttatttgtac cgtaggtaat
      841 atctgcttgg tatgcttttt tacgtgcttc tgagttaggt tcatgattat caatacagtc
      901 tacgcgtaat ccatggaatt caaatattgg tcccatccat tcagagtcgc gttttgctaa
      961 atagtcgttt actgttacta catgaactcc tttaccgggt aatgcattaa gaaaaacagg
     1021 taatgttgca actaatgttt tcccttcgcc tgttgccatt tctgcaattt taccttgatg
     1081 taatatagta ccacctatta gttgcacttc atagtgaatc atgtcccaag ttatcatatt
     1141 accaccggcc aaccatgaat ttttccatat agctttatca ccttgtattg taatatggtc
     1201 tttgcgagta gctaattctt tgtcataatc taaagcagta acttctacag ttgtattatg
     1261 tgtaaatcgt tttgctgttt cttttacaac agcaaaggct tgtggtaata tttcttctaa
     1321 tgttttttca attgttgtat caatttcttt tttgagtgaa tctattgaat catacatttc
     1381 ttcgcgtttt gcaaaactaa gattctcatc attgtctaaa ctgttttgca atgaatctat
     1441 ttgagtttgc ataggtttta cggcttcttg tataatagtg cgtagtttaa cagtaacgtt
     1501 acgtaactca tcatgtgaga gaccggaata ttctgattca aattctttga ttttttgaac
     1561 aagggggagt attgcttgta tatctctttg tgatttattt ccaaggaatg cttgaagtat
     1621 tttttgaact aaactcatgt gataattatt aaaaaaagtt gttttttata aaaagcaaat
     1681 atacaattta gtttgaaagt aactaatagt ttaatatatt caatatgtat tatgaattaa
     1741 aaatataatt gaattctatt ggagttaaat atagaatttt tatatctgta atacgttgaa
     1801 gttatagtat ttggcaatta tttttgaggt aaaaattgaa aaaatgtatc gcctcgcaat
     1861 cctaatcgca atgcttctaa tgctaacacc tcatttgttg caatatttcc taaatttacg
     1921 ttatatccaa gtaattgaat aaatagagct tgctgtgctt ttagtggggc ttcccacatt
     1981 acttttgtaa tatcaatatt tgaaattatg gtatttataa gagttttgtt aggagagccg
     2041 tctttattga aaattcctat tgttccactt tctcttgctt cggttattac ttttactgaa
     2101 ccggcgtcaa gttcactttg catttgcaaa atccattctt cgggagtaat tactacgctt
     2161 gcatcttttg agccaacttc agaaattact gtgaaatgtt ttgataattg tgatatgtat
     2221 gaaagtttgt cttcatgttt catattcata gaaccatcgc ttacttcggc tacttctatg
     2281 ccatatgtat ctaaaaaacg ttggtattca tcaaattggt tacgaataat aaaaagttca
     2341 aatagagtac ctccaaaata gggaataatg ccggcttctt tatatattgc tatttttttt
     2401 tctaaattat gtgtaatgag tgaggttccg aatccaagtt ttgcaaaatc aatatattca
     2461 tgaacatctt gagccaatcc ttgagcttct atagaggtta accctttatc aataatcatt
     2521 gtttgacctg catttcgtgg ttttttttcg cgttgaggaa tataagacag tggaaaattc
     2581 attatagtat ttattttaaa atttcattgt aaaaaagttg taatgaatta agtggaagat
     2641 gacaaatatc aaaaaagtac atggttgcat attcaggatc taaactatat ccttttttga
     2701 aataatcaat tgcttttgga atatcatgtt cgttggcatg atatgcagct aatgtaaagt
     2761 ataaagtagc atgtggggct gcttctatgc cctgacgtaa tattttttta gccacatttg
     2821 gattgtttaa ttccatttct atttggctat attgtatcca agcttcgaat tcaaatttat
     2881 taatagctat agcttgttta aaaatttgaa tagctttagg atacatgccg caatcactta
     2941 aagctatacc ataacttatt aaatatttat cgttttgtga atctaattct aaagcttttt
     3001 caaataattc taatgctttt tgtgtattgt tattataaaa atgcagcaat gctatgccat
     3061 accatccttc accatattca ggggctattt cggtaatttt ttcataagtt gccatagaat
     3121 gttctatttc tcctagtttt tcataacatt cggctatata ttgtaatgca tatatatttt
     3181 caggctcaaa ttgtaaaaca tcttggtaac acagaatagc ttcattatag agttcttttg
     3241 tcatatagca gtttgctttg ttaaaatgag cagagctaaa tgattcattg atagtaattg
     3301 cataatcaaa tgcctgaata gcttcatcta tttcttcaga ttgtaaataa accaaagcta
     3361 aattgaacca tgcatattct gaatatggat cggc
//
LOCUS       DAOTOW010000174         3384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22100_length_3384_cov_2.686392, whole genome shotgun sequence.
ACCESSION   DAOTOW010000174 DAOTOW010000000
VERSION     DAOTOW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22100_length_3384_cov_2.686392"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..892
                     /gene="tsaB"
                     /locus_tag="P5243_04115"
     CDS             185..892
                     /gene="tsaB"
                     /locus_tag="P5243_04115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRS18667.1"
                     /translation="MSSIRILSIETSTSICSVALHSNGVCVDCIEINEQNAHSSKLTV
                     LMEQLLEQNNVRIADCDAIAISGGPGSYTGLRIGTSVAKGLCYAANLPLIAVDTLQAL
                     ALACKGTIESKQNIDNNTIFMPMIDARRMEVYTSQWNTNMKQICDTFPLIVDTETIHT
                     FDSSYTYVLCGDGSAKCKALLNAPHIHFVENCNTTAETVGTLAYKKFQNNQFEDVAYY
                     EPFYLKEFVTTTPKNKM"
     gene            complement(899..1666)
                     /locus_tag="P5243_04120"
     CDS             complement(899..1666)
                     /locus_tag="P5243_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRS18668.1"
                     /translation="MVNKLTYGVSLSGGGARGIIQLGVLHALDEYGIVPSIVAGTSMG
                     AINGVFYAAGYKPQEIAHILKTHLKTLSFSWLLWVRSGFKSFDTLRHVLEQYIASDNF
                     DALTYPLYISVTNLNSGKNELISSGPLFDFVIASASIPIIYKPKFINGCYYVDGGLTN
                     NFPARVLQGKCDKIIGIHVNHIEEVQEFPNLVSLVERMYRIGIYSNVSHKISLCDYFI
                     DPPEARKYETFDFDKFDEIYDLGYKKGLELVKKIKEK"
     gene            1793..2461
                     /locus_tag="P5243_04125"
     CDS             1793..2461
                     /locus_tag="P5243_04125"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRS18669.1"
                     /translation="MNIQEEFSKAIQFIAHHQDGEIAETLKYRGLEYNMIYGVSSNIL
                     YGFAKTIEKNQELAMLLWNEDFREAKLLAFMVANPETINNQQLETMVQGCINHEMVEI
                     GALHLFSKIPNAIQKSYEWAQSEEEYVKMTAYMIISHLAIRLKNVKFSDLAKFLPLYE
                     RDFTHGSYFVRKTLVNSFQEVAFRKPGIKAPIVKATKKLLEQNIGTEFELQAKDMLHV
                     LNYC"
     gene            2481..3260
                     /locus_tag="P5243_04130"
     CDS             2481..3260
                     /locus_tag="P5243_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRS18670.1"
                     /translation="MLEKSSNPVFSKSTFAPSLSSTQQGVMTLQGTINKSIILLLTII
                     VSASLTWNMVQQGNASVGGLMMGGLILGFITAMVTIFKKEWAGITAPIYAVFEGLFLG
                     GVSAFVEAAFFASKEPGVASTSNGIVFNAVALTFGVFLLMLLLYRNQIIKPTQKFMMG
                     VAAATGAIFLLYLVNMIMGFFGASMPFLHSNGPLGIGISLVIVVVAALNLIIDFKNIE
                     DAVAQGAPKYMEWFGAFGLMVTLVWLYLEILRLVALLTGRD"
BASE COUNT         1170 a          551 c          574 g         1089 t
ORIGIN      
        1 gatgtattat ttgccggcag tataggacga agcgacttgc cgggtggcaa tcatgaacaa
       61 ctaattgacg gtattactaa aaaacttatg atactgcccg acgatgttat tgtgtattca
      121 ggacatgggc ctactacaag cattttggta gaacgaaata caaatcctta tttttaatac
      181 aactatgagc agtatcagaa ttttgagtat agaaacatct accagtatat gttcagttgc
      241 attgcatagc aatggtgtat gtgttgattg tattgaaatt aacgaacaaa acgcacattc
      301 ttcgaaactt acggtgctta tggaacagct attggagcaa aacaatgtgc gtattgccga
      361 ttgtgatgct attgcaataa gtggcggtcc cggttcgtat acaggtttgc gtataggcac
      421 ttcggttgct aaaggcttat gttatgctgc aaatctgcca ttgatagctg tagacacctt
      481 gcaagctctt gcacttgcgt gcaaaggtac aatagaatca aaacaaaata tagataataa
      541 tactattttc atgcccatga tagatgctcg gcgtatggag gtatatacat cgcaatggaa
      601 tacaaacatg aaacagattt gtgatacatt tccattaata gttgatacag aaaccataca
      661 tacatttgat tcatcatata cctatgtgtt gtgtggcgat ggaagtgcaa aatgtaaagc
      721 cttacttaat gctccccata tacattttgt agaaaattgt aataccactg ccgaaacggt
      781 tggaacactt gcctataaaa aatttcaaaa caatcaattt gaagatgttg cctactacga
      841 accattttat ttaaaagaat ttgtaacaac aacaccgaaa aataaaatgt aagcggactt
      901 atttttcctt gattttctta actaactcca atcccttttt gtaacccaag tcatatattt
      961 cgtcgaattt gtcgaaatcg aatgtttcat acttacgagc ttcgggaggg tcaataaaat
     1021 aatcgcatag cgaaattttg tgtgatacat tactgtaaat accaatacga tacattctct
     1081 caaccaacga aactaaatta gggaattctt gaacctcttc aatgtgattt acatgaatcc
     1141 caatgatttt atcgcatttg ccttgtaaca cacgagcggg gaaattatta gtcaaaccac
     1201 catcaacata gtaacaacca ttaataaatt ttggtttgta tatgattgga atagatgctg
     1261 atgctataac aaaatcaaac aaaggccctg atgatataag ctcatttttt cctgaattta
     1321 aattagttac ggatatatac aaaggatatg tgagtgcatc aaaattatcg gaagcaatat
     1381 actgttcaag aacatggcga agtgtgtcaa aacttttaaa tccggaacga acccacagca
     1441 accatgaaaa tgacaaagtt ttaagatgtg ttttaagaat gtgagctatt tcttgtggtt
     1501 tatatcccgc ggcataaaac actccattga tagcccccat acttgtacct gcaacaatag
     1561 atggaactat accatattca tctaatgcat gcaatacgcc caactgaata attccgcgag
     1621 ctcctcctcc actaagtgaa acaccatatg taagcttatt taccatatca atcaaattgt
     1681 tttatgaaac ctgtcagttt ttttaaccaa ttatactatc attaaaaaaa tgatttataa
     1741 agccaatgta atatacaaaa ttcttttata tttttatcaa aattttttac atatgaatat
     1801 acaagaggaa ttttcaaaag ctattcaatt tattgcacat catcaagatg gtgaaattgc
     1861 agaaactctc aaatatagag ggttagagta caatatgata tatggcgttt catctaatat
     1921 tctgtatgga tttgcaaaaa ctattgaaaa aaatcaagaa ttagccatgt tattatggaa
     1981 cgaagatttt cgcgaagcta aattacttgc atttatggtt gccaatcccg aaactattaa
     2041 taatcaacaa ttagaaacta tggttcaagg gtgtataaat catgaaatgg ttgaaatagg
     2101 agctttacat ttattttcta aaattccaaa tgcaatacaa aaatcatatg aatgggcaca
     2161 atcagaagag gaatacgtca aaatgacagc atatatgata atttcacatc ttgctatacg
     2221 cttaaaaaat gtaaaatttt ctgatttagc taaatttttg cctttgtatg aacgcgattt
     2281 tactcatggc agttattttg taaggaaaac tctcgtaaat tcgttccaag aagtagcttt
     2341 tagaaaaccg ggtattaaag ctcctattgt aaaagcaaca aaaaaacttt tagaacaaaa
     2401 tattgggaca gaatttgagt tacaagcaaa agatatgtta catgttttaa attattgtta
     2461 attattaaaa ttttatagat atgttagaaa aatcatcaaa tccggtattt tcgaaatcaa
     2521 catttgctcc atcattaagt tcaacacaac aaggagtaat gacgttgcaa ggtactataa
     2581 ataaaagtat catactatta cttacaatta ttgtaagtgc ttctcttacc tggaatatgg
     2641 ttcaacaagg aaatgcatcg gtaggtggac ttatgatggg tggacttatt ttagggttta
     2701 ttaccgctat ggttactata ttcaaaaaag aatgggcagg gattacagca cctatttacg
     2761 ccgtattcga agggttattt ttaggtggtg tatcagcatt tgtagaagca gcattttttg
     2821 cttctaaaga gcccggagta gcttcaactt caaatggaat agtttttaat gctgtagctc
     2881 tcacatttgg agtatttttg ctcatgcttc tactctatcg taaccaaata attaaaccta
     2941 cccaaaaatt tatgatgggt gtagcggctg ctaccggagc catattttta ctatacttag
     3001 taaatatgat aatgggattt tttggtgcaa gtatgccatt tttacacagc aatggtccac
     3061 ttggtatagg tattagttta gtaatagttg ttgtagcagc actcaacctt atcatagact
     3121 tcaaaaatat agaagatgcg gttgcacaag gagcacctaa atatatggaa tggtttggtg
     3181 catttggttt aatggttact ttagtgtggt tatatttaga aatattacga ttagttgctt
     3241 tactcacagg tagagattaa taacgtacaa acaatattaa aaaaagctgt tatacattca
     3301 gtataacagc tttttttttc tataacaata caaaattctc taaatttaca tccatgttta
     3361 ctatataaat tacaatgaaa tcca
//
LOCUS       DAOTOW010000175         3383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22111_length_3383_cov_2.728365, whole genome shotgun sequence.
ACCESSION   DAOTOW010000175 DAOTOW010000000
VERSION     DAOTOW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22111_length_3383_cov_2.728365"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2210
                     /gene="nifJ"
                     /locus_tag="P5243_04135"
     CDS             <1..2210
                     /gene="nifJ"
                     /locus_tag="P5243_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRS18671.1"
                     /translation="FAYDSKKSGGITISHLRFGKKPITSTYLVSNPTFVSCSEPSYLT
                     KYDMLKGIQKGGTFLLNALWDAKDIEKHLPNKIKKQLAVNQVKFYIINATKVAEEIGL
                     GNRTNSILQSAFFKLANVIPYEDAVGFMKAAIKKTYGKKGDDIVAMNCKAVDMGVESL
                     QEVPVKPEWAQLPDGEELVIYQGITEKTEKELASFVKNIAGVMNKQEGDNLPVSAFRG
                     REDGTIPNGTSQFEKRGIAVHVPEWQSDNCIQCNQCAYVCPHAVIRPFLLDEKEMAGA
                     PAGLKTLTPLGKGYEGLQYKIQISPLDCTGCGSCANVCPGKKGEKALVMKPLDSQLAT
                     EKVNWDYISNNVTYKDTISDKTANVKNSQFARPLFEFSGACAGCGETPYVKLITQLFG
                     DRMTIANATGCSSIYGGSSPASPYNVNEQGKGVAWANSLFEDNAEFGLGMNIAMTKLR
                     ERIATKMEESKNDVPAELQAAFAEWKEGMHDAAASQVATDKLVPLLEKSNAPIAKDIL
                     ALKQYLSKKSNWILGGDGWAYDIGFGGLDHVLASGADVNILVLDTEVYSNTGGQASKS
                     SPTGSVAKFATSGKPVRKKDLGRMAMAYGYVYVAQIAMGANQAQTLKAIREAEAYPGP
                     SLIIAYAPCIAHGIKGGLTGSQTEEKNAVECGYWHLYRFDPRLEEEGKNPFQLDSKEP
                     NFDEYMNFLKNEVRYTSLAKTFPNEAQALFNQSLEDAKWRYKQYRLLSEMKFE"
     gene            2378..>3383
                     /locus_tag="P5243_04140"
     CDS             2378..>3383
                     /locus_tag="P5243_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18672.1"
                     /translation="MKASKVLHKGVARIAIYFAHNQQIANNLRTIPDARWSKTLGAWH
                     IPYTKQAFEQLQILFPNIEYPITKHEKTTDTEHTNNITHILTEQKQANTQKTDKKSVT
                     IDVIGRKIILKMPKVDVDVQFVRSLLYSTWDKTEFCWIIPNYGANLDLLKDYFKQRIT
                     RITEHEQYGIETKSGKTYTVTKNEIIVIKTGNGRLKLLCNYNTNIAKVIKTMPFHTWD
                     AKNKWWTIPNSEQLLQQIQAVANDCKMIVTIQEEEPCKDKTARITAFDIPNYRKCPSE
                     MQEKLIELRYSNNTLRTYTNAFEEFINYYNRYDISAIDERMIIAYIRYLVTERKISES
                     Y"
BASE COUNT         1243 a          569 c          654 g          917 t
ORIGIN      
        1 attttgcata cgactctaaa aaatctggtg gtattacaat ttctcactta cgttttggta
       61 aaaaacctat tacttctacg tatttagtaa gcaacccaac atttgtatcg tgttctgagc
      121 catcgtattt gaccaaatat gatatgctca aaggaattca aaaaggtgga acttttctgt
      181 tgaatgcatt atgggatgca aaagatatag aaaaacattt accaaataaa atcaaaaagc
      241 aattagctgt aaatcaagtt aaattttata taataaatgc tacaaaagtt gctgaagaaa
      301 ttgggcttgg aaacagaaca aactctattt tacaatcggc attctttaaa ttggcaaatg
      361 taattcctta tgaagatgca gttggtttta tgaaagctgc tattaaaaaa acatatggta
      421 aaaaaggtga cgatattgtt gcaatgaact gcaaagcagt tgacatgggg gttgaaagtt
      481 tacaagaagt acctgtaaaa cctgaatggg ctcaattacc tgatggtgag gaattagtaa
      541 tttaccaagg aattaccgaa aaaaccgaaa aagaacttgc ttcgtttgtt aaaaatatag
      601 caggtgtaat gaacaaacaa gaaggagata atttaccggt gagtgcattc agaggtcgcg
      661 aagacggtac tattccaaat ggcacatcgc aatttgaaaa acgcggtatt gcggtacatg
      721 tacccgaatg gcaatcagac aattgtattc aatgtaacca atgtgcctat gtgtgtcctc
      781 acgcagtaat tcgtccgttc ttactcgatg aaaaagaaat ggcaggagca cctgcaggat
      841 taaaaacgct taccccgctt ggtaaaggat acgaaggatt acaatataaa atccaaattt
      901 cgccacttga ctgtaccggt tgcggtagtt gtgcaaatgt gtgtccgggt aaaaaaggtg
      961 aaaaagcatt ggtaatgaaa cctcttgatt cacaattggc tactgaaaaa gtaaattggg
     1021 attatatttc aaacaatgtt acatacaaag atacaatcag tgataaaacc gcaaacgtta
     1081 aaaacagcca gtttgcacgt ccgttatttg aattctcggg tgcatgtgca ggttgtggcg
     1141 aaacaccgta tgtaaaactt attacacaat tgtttggcga tagaatgact attgcaaatg
     1201 caaccggttg ttcttctatt tatggtggtt cttcgcctgc aagtccatac aatgtaaacg
     1261 agcaaggtaa aggtgtagcg tgggctaact ctttgttcga agacaatgct gaatttgggt
     1321 tgggtatgaa catagctatg acaaaattgc gtgagcgtat tgctaccaaa atggaagaaa
     1381 gcaaaaacga tgtacctgca gaattgcaag cagcatttgc tgaatggaaa gaaggcatgc
     1441 acgacgcagc agcatcacaa gttgctaccg ataaattggt tccactctta gaaaaaagta
     1501 atgctccaat agctaaagat attcttgcat tgaaacaata tttatctaaa aaatcgaatt
     1561 ggatacttgg tggcgacggt tgggcttacg atataggttt tggtgggtta gaccacgtat
     1621 tggcttcggg tgccgatgtt aatattttag tactcgatac cgaggtatat tctaacaccg
     1681 gtggacaagc atcgaaatca tcgccaacag gttcggttgc taaatttgca acatcgggta
     1741 aaccggtacg caaaaaagac cttggacgta tggcaatggc atacggatat gtatatgtag
     1801 cacaaatagc tatgggtgca aatcaagcac aaacgttgaa agctattcgc gaagcagaag
     1861 catatccggg accatcgtta attattgcct atgctccatg tatagcacac ggtatcaaag
     1921 gtggattgac aggttcacaa accgaagaga aaaatgcggt agaatgtgga tactggcact
     1981 tatatcgttt cgacccacga ttagaagaag aaggcaaaaa tccattccaa ttggattcaa
     2041 aagagccgaa ttttgatgaa tatatgaatt tcttgaaaaa cgaagttcgc tatacatcat
     2101 tggctaaaac cttccctaac gaagcacaag cattatttaa ccaatcgttg gaagatgcaa
     2161 aatggcgtta caaacaatat cgtttgttga gcgaaatgaa atttgaataa cattaattca
     2221 aagaatacag aagaaaagag gaagcgaatg tttcctcttt tttttgtata tctcgcaagt
     2281 gaaagttact ttctattttt ttttgcatac ttgcatataa tgtattaatt ttggacttta
     2341 tcaaccagta tacgccgcat ttttttaata tattactatg aaagcttcaa aagttttaca
     2401 caaaggtgta gcaagaattg ctatttattt tgctcacaat caacaaattg caaacaatct
     2461 acgcactata ccagatgctc gatggagcaa aacattgggt gcatggcata ttccttatac
     2521 caaacaggca tttgaacaat tacaaatatt atttccgaat atagaatatc ctataacaaa
     2581 acatgagaaa acaacggata ctgaacatac aaataacatt acgcacatac tcacggaaca
     2641 gaaacaagcc aacacgcaaa aaactgacaa aaagagtgtt acaattgatg taataggcag
     2701 aaaaataatt ttaaaaatgc ctaaagtcga tgttgatgtt caatttgttc gttcattact
     2761 atatagcaca tgggacaaaa ctgagttttg ttggataata cctaactatg gagcaaattt
     2821 agacttactc aaagattatt ttaaacaacg aattacgaga attaccgagc acgagcaata
     2881 cggcatagaa acaaaatcgg gcaaaacata tactgttaca aaaaatgaaa tcatagttat
     2941 aaaaacggga aatggcagac tcaaactatt atgtaattat aatacaaata tagcaaaggt
     3001 tataaaaaca atgccgtttc atacatggga tgcaaaaaac aaatggtgga caataccaaa
     3061 ttcggagcaa ttgttacaac aaatacaagc ggttgccaat gattgtaaaa tgattgtgac
     3121 tattcaagaa gaagaaccat gcaaagataa aacagcacgc attacagcat ttgatatacc
     3181 aaattacagg aaatgcccga gcgaaatgca agagaaactt atagaattac gatatagcaa
     3241 caacacccta cgaacataca caaacgcatt cgaagaattt ataaattatt ataatagata
     3301 tgacatatcg gctatagacg agcgtatgat aatagcatac attcgctatt tggtaacaga
     3361 acgaaaaata tcagaatcgt atc
//
LOCUS       DAOTOW010000176         3359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22313_length_3359_cov_3.370460, whole genome shotgun sequence.
ACCESSION   DAOTOW010000176 DAOTOW010000000
VERSION     DAOTOW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22313_length_3359_cov_3.370460"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="P5243_04145"
     CDS             <1..657
                     /locus_tag="P5243_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS18673.1"
                     /translation="PEIKFLQQLREICDIYGALLIFDEVMTGFRVAKGGAQELYSIVP
                     DITTLGKIIGGGLPVGAYGGKKNIMDCLSPKGPIYQAGTLSGNPLAMTAGYVTLSLIQ
                     QNETFYKELETKAAMVEEGFTYNIQKTGIPAVINRVGSMFTMFFTEAAHVSTYKDVCT
                     CDTNKYAAFYTFSRDNGLYLAPSQFEAAFISIAHSFADIEKTIEAHYNALNHIKNLYA
                     "
     gene            654..1694
                     /gene="hemE"
                     /locus_tag="P5243_04150"
     CDS             654..1694
                     /gene="hemE"
                     /locus_tag="P5243_04150"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRS18674.1"
                     /translation="MTDSNALLLQTLRGEKTHRPPLWFMRQAGRILPNYNHLKQQYSF
                     KELMEQPQLAAQVTLMPIEDLHVDAAILFSDILVIPQAMGMTLEFPESGPVFSNPLMK
                     YTNPHSILKPDADKLSYIYSVIDAITATKPNHIPLIGFCGSPFTVLLYMLQGLHGKSD
                     FPDAIQYIYSNQKTVAILLEQITELSIEYMQQQINHGIDVFQLFDTHAGLIPFELYKT
                     LILPHVEHMCTKARQLNTPFIYFPKGIGVGISYITPHYCDFLSIDWQTPLHIARKIVH
                     KSIGLQGNIDPRILYASEQEISHHLEYYREFGKHNYNWICNLGHGFMPGLDYNKALHL
                     VKTLQEFDWQRY"
     gene            1723..2553
                     /gene="pyrF"
                     /locus_tag="P5243_04155"
     CDS             1723..2553
                     /gene="pyrF"
                     /locus_tag="P5243_04155"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRS18675.1"
                     /translation="MNKQELIQQIQHKRSFLCVGLDTEITKVPQHLLQTNDPIFEFNK
                     QIIDATAPYTVAYKPNIAFYECYGIQGWQSLEKTVRYITQNYPDIFVIADAKRGDIGN
                     TSKMYAQAFFENLPFHSVTVAPYMGEDSVTPFLSYEGKWVILLALTSNKGAFDFQCIE
                     SNGIPLYKQVITQSQRWGTTENMMYVVGATKANMLKDIRSVAPNHFLLVPGVGAQGGS
                     LQEVAQYGMNAECGLLVNSSRAIIFADASEQFAAVAGIKAQEMQIEMEELLRKYSSLI
                     "
     gene            complement(2550..3092)
                     /locus_tag="P5243_04160"
     CDS             complement(2550..3092)
                     /locus_tag="P5243_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRS18676.1"
                     /translation="MNSIVAVNHYSDILPQYRETPISLLFEYHNFKIPFKIYHTAQLL
                     VGMCMDNRKYLHIPDNFAYILRSGGGNLRNSEFKVSYAIAVGNISYIALIGHTQCGMV
                     NLAQRKQLFISGMVKNAGWNYMQAEQHFDTYSKQFEIENEIDFTVQESIRLQATYPKV
                     TVVPLLFKVEDSKLYQIVQH"
BASE COUNT         1139 a          591 c          527 g         1102 t
ORIGIN      
        1 cctgaaatta aatttttaca acaattacgt gaaatatgtg atatttatgg tgcattactc
       61 atttttgatg aagtaatgac cggttttcgt gtagcaaaag gaggagcaca ggaattatat
      121 tctattgttc ccgatattac tacactcgga aaaattattg gaggtggatt acctgttggt
      181 gcatacggcg gcaaaaaaaa tattatggat tgcctttcac ccaaggggcc tatataccaa
      241 gcaggaacat tatccggcaa ccccttagca atgactgccg ggtatgttac tttgtcacta
      301 attcaacaaa atgaaacatt ttataaagaa ttagaaacaa aagctgctat ggttgaagaa
      361 ggatttactt ataatattca aaaaaccgga attcctgctg taattaatcg tgttggctct
      421 atgtttacta tgttttttac cgaagctgct cacgtttcaa catacaaaga tgtatgcact
      481 tgtgatacaa acaaatatgc agctttctat acattttcgc gtgacaatgg actatatctt
      541 gctccatcac aatttgaagc tgcatttatt tcaatagcac actcttttgc tgatattgaa
      601 aaaactattg aagctcatta taatgcttta aaccatatta aaaacttgta tgcatgaccg
      661 attcaaatgc tcttttatta caaacactcc gtggagaaaa aactcataga cctcccctat
      721 ggtttatgcg tcaagcaggc agaattttac ctaattataa tcatttaaaa caacagtatt
      781 ctttcaaaga attgatggaa caaccacaat tggctgcaca agttactctt atgccaattg
      841 aagatttaca cgtagatgcc gctattttat tttccgatat tcttgtaatt ccccaagcta
      901 tgggtatgac cttagaattt cccgaatcag gtccggtatt ttcaaatccg ctcatgaagt
      961 ataccaatcc tcacagtata ctaaaacctg atgcagacaa actttcatat atttattcgg
     1021 taattgatgc aattactgca acaaaaccaa atcatatccc actaattggt ttttgcggtt
     1081 ctccttttac tgttttactc tatatgttgc aaggattaca cggcaaatca gatttccccg
     1141 atgctataca atatatatat agcaatcaaa aaactgtagc aatattactt gaacaaatta
     1201 ccgaattatc aatagaatat atgcaacagc aaataaacca cggtattgat gtatttcaac
     1261 tgtttgatac tcatgcaggt ttaattccct ttgaattata taaaacactt atattacctc
     1321 atgtagaaca tatgtgcaca aaagctcgtc aactcaatac tcctttcata tattttccta
     1381 aaggtatagg tgtaggtatt tcttatatta ctccacatta ttgtgatttt ttgagtattg
     1441 attggcaaac tcctttacat atagcaagaa aaatagttca caaatctatt ggcttacaag
     1501 gcaatattga cccacgaatt ttatacgcat ctgaacagga aatttcacat catttagaat
     1561 attacagaga atttggaaaa cataattata actggatttg caatttaggt catggcttta
     1621 tgcccggttt agattataac aaagctctac atttggttaa aactttacaa gaatttgatt
     1681 ggcaacgata ctaaatacat tttatataca atactttata ctatgaacaa acaagaactc
     1741 attcaacaaa tacaacacaa gcgtagtttt ttatgtgtag gccttgatac cgaaattact
     1801 aaggttcctc aacacctatt acaaactaac gatccaattt ttgaattcaa caaacaaata
     1861 atagatgcta cagctccata tactgtggca tacaaaccca acatagcatt ttatgaatgt
     1921 tatggaatac aaggatggca aagtttagaa aaaacagtaa gatacattac acaaaactat
     1981 cccgatattt ttgttatagc cgacgcaaaa cgtggcgaca taggcaatac atcaaaaatg
     2041 tatgcacaag cattttttga aaatttacca tttcattcgg taactgttgc tccatatatg
     2101 ggcgaagatt ctgttacccc atttttatca tacgaaggca aatgggttat actgttagca
     2161 cttacttcaa acaagggtgc ctttgatttt caatgtattg aaagtaacgg aataccattg
     2221 tacaaacaag taattacgca atctcaacgc tggggcacaa cagaaaatat gatgtatgta
     2281 gtaggtgcaa ccaaagcaaa tatgttaaaa gacatacgaa gtgtagctcc caatcatttt
     2341 ttattggtac ccggcgtggg tgctcaagga ggtagtttac aagaagttgc tcaatatggc
     2401 atgaatgcag aatgtggttt attggtaaat tcttcaagag caattatttt tgccgatgcc
     2461 agcgaacaat ttgctgcagt tgccggtatc aaagctcaag aaatgcaaat agaaatggaa
     2521 gaattattac gaaaatattc ttctcttatt taatgttgca ctatttggta caatttggag
     2581 tcttcaactt taaacaataa aggaacaact gtaactttag gatacgttgc ttgtaaacga
     2641 attgattctt gcacagtaaa atcaatttca ttttctattt caaattgttt tgaataggta
     2701 tcaaaatgtt gttcggcttg catgtaattc caacctgcat tttttaccat tcccgaaata
     2761 aataattgtt tacgctgagc taaatttacc ataccgcatt gcgtatgacc tataagagca
     2821 atatatgata tattgccaac agctatagca tatgatactt taaattcgct atttcgcaag
     2881 ttcccaccac ccgatcttag aatataggca aaattatcgg ggatatgtaa atactttctg
     2941 ttatccatac acataccaac tagtaattga gctgtatgat atattttaaa tggaattttg
     3001 aaattatggt attcaaacag taacgagatt ggcgtttcac gatattgagg aagaatgtct
     3061 gaataatgat taacagcaac tatcgaattc ataatttttg ttgcaagtta cacgatttca
     3121 ttacgcaaat ccaaaacaaa agagtgcaag ttaatttgta aaaaatatga tttacaaaag
     3181 aaaaaataat aacgtaatct atttgatttg tttaattttt tggagcttga atagtcttat
     3241 attttttatt ttcgcgagtt cctcctttag aatttacctg accaaatttt ggcacttttt
     3301 tatttcctgc tccttgcata ctactattaa acataggtaa cgctttttgt ggtttttct
//
LOCUS       DAOTOW010000177         3344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22447_length_3344_cov_2.370933, whole genome shotgun sequence.
ACCESSION   DAOTOW010000177 DAOTOW010000000
VERSION     DAOTOW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22447_length_3344_cov_2.370933"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1504)
                     /locus_tag="P5243_04165"
     CDS             complement(56..1504)
                     /locus_tag="P5243_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRS18677.1"
                     /translation="MITFLVSLLILILGYVWYSRIIERIFGISKHTQTPAYTKTDGVD
                     YVPMHWSKTLLIQFLNIAGLGPIFGAIAGAMWGPVVFIWIVLGCVFAGAVHDYLSGLI
                     SLKQQGASIPEIIGMYLGNPVKQGMRVFTVFLMIVVGAVFIVGPAKILFGIAGGAISM
                     NVWIAIIFVYYILAAVLPIQAIIGKLYPIFGIVLICMAFAILFGLWYYGYSIPELQWH
                     NFRNMHNNPEQFLIFPMLFVTVACGAISGFHATQSPLMARCITNETEGRRVFYGAMII
                     EGIVAVIWAAIGMSFFGGVSQLQAVMNEHGGNAAWVVDTMSKTMLGKFGAIMVLLGVV
                     FAPITSGDTAFRSARLIISDIFSIPQKMKYRILVSIPIFSAGFVLSQIDFSIIWRYMA
                     WANQTLAAVVLWAITSYLYTHSKNYWITFIPALFMTTVSCVYIFVAPEGFYIPMNIGI
                     IAGLSVTAIVLFLFIRYILKKSRNNSHVFHTE"
     gene            complement(1504..2241)
                     /locus_tag="P5243_04170"
     CDS             complement(1504..2241)
                     /locus_tag="P5243_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRS18678.1"
                     /translation="MRYIVLCVFCSLSFMLQAQVQKVSKQYLNEYFDVCDSSVAKYIA
                     TIYYSDTVLSKASVVISNLKDNIVSECYYSNYVLGIKHGECKSYHENGKIKSIAHYDS
                     GLLHGEVLCYFAGGQLKRRDVYKHNILQSGTCYGLNGNEIKYFPYLVAPEYPQGLKNL
                     YKFIYIHTNYPDEAKRLGVKGTVIVRLFINKKGEITKYEIQKSVHPLLDAEALRVCKM
                     LGNFKPGQEDGEYTNSIFVLPIKYEFE"
     gene            complement(2324..>3344)
                     /locus_tag="P5243_04175"
     CDS             complement(2324..>3344)
                     /locus_tag="P5243_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS18679.1"
                     /translation="ELPIGFYTFDLYDRNIVKPDVLECTSDTAKNRQFEVAPAAKTKI
                     VGDTLACVNTVEVMYTVETVPSVASTYIWSVTGNRLNYSKDANSSAVRYIDWDIQGVD
                     TITVYENTYAGCEGFDTLIVKVANYPVPNFTFTMPGASNVVEYLDSTTQKNVTDKLAD
                     GTVVEYPVTYTMYWNFAKTKDDDLVDLVVDYKDRKKPVTVGGYVVGPKYPILTVENSY
                     GCKAKVSKEVYIDVLGNIYVPTAFAPTNPAAAVRVFQPVTYGVEKCEVWIFDKWGNLI
                     WYSDEVEDGIFVGKWDGTYNGEMLQSDTYIWKIEANYVGGNEWTGLKKPNGTETKFGS
                     VVLLR"
BASE COUNT         1149 a          668 c          416 g         1111 t
ORIGIN      
        1 tgaacttttg tggaaaccat tgtcattatc gatttttttt aataattaaa ttatattatt
       61 ctgtatgaaa aacatgagaa ttatttcgag atttttttag aatatatcga ataaataaaa
      121 ataatacaat agcagttaca ctaagacctg ctattatccc aatattcata ggtatataaa
      181 atccttcggg tgcaacaaaa atatatacgc agctcacagt tgtcataaac aaagcaggaa
      241 taaaggttat ccaataattt ttggaatgag tataaagata tgaggtaatt gcccatagca
      301 ccactgctgc taatgtttga tttgcccatg ccatataacg ccatattatt gaaaaatcaa
      361 tttgcgataa tacaaatcct gcactaaaaa ttggaatact aaccaaaatt ctatatttca
      421 ttttttgtgg aatagaaaat atatcagata ttattaaccg agcacttcta aaagcagtat
      481 ctccggaagt tataggagca aatacaacac ctaacaaaac cataattgca ccaaatttac
      541 caagcattgt tttactcatg gtatcaacta cccaagctgc atttcctcca tgttcattca
      601 ttactgcttg caattgagaa acaccaccaa aaaaactcat tccaatagct gcccatatta
      661 cagcaacaat cccttctata atcattgctc cgtaaaacac acgtcgtcct tcagtttcat
      721 tggtaataca cctagccata agaggtgatt gcgttgcatg gaaacccgaa attgctccac
      781 aagcaactgt tacaaataac ataggaaaaa ttagaaattg ttctgggtta ttgtgcatat
      841 tcctaaaatt atgccactgt aattctggta ttgaatatcc ataataccat aaaccaaaca
      901 aaattgcaaa tgccatacaa ataagaacaa ttccaaaaat tggatataat ttacctatta
      961 ttgcttgaat tggtaataca gcagcaagaa tataatatac aaaaataatt gcaatccata
     1021 cattcataga aatagcacct cctgctatac caaataaaat ttttgcagga cctacaataa
     1081 acactgcacc tacaactatc atcaaaaaaa ccgtaaacac tcgcatgcct tgttttacag
     1141 gatttcctaa atacatacca atgatttcgg gaatactcgc tccttgttgt tttaacgata
     1201 tcaatcccga caaataatca tgaacagcac ctgcaaatac acaccccagc actatccata
     1261 taaacaccac aggtccccac attgctcctg caatagcacc aaatatgggt cctaatcccg
     1321 caatatttaa aaattgaata agtaatgttt ttgaccaatg cataggaaca taatcaactc
     1381 catcagtttt tgtataagca ggtgtttggg tatgtttaga aattccgaat atacgttcta
     1441 ttatacgtga ataccaaacg tatcctagta ttaatatcag taaagaaaca agaaatgtta
     1501 tcattattca aattcatatt ttataggtaa tacaaagatt gaatttgtat attcgccatc
     1561 ttcttgtccc ggtttaaaat tacccaacat tttacatact cgcaatgctt cggcatcaag
     1621 aagagggtga acagactttt ggatttcata ttttgtgatt tctccttttt tatttataaa
     1681 taggcgaact attaccgtac cttttacacc taaacgttta gcttcatcag gataatttgt
     1741 atgaatatat ataaatttat ataaattctt caaaccttgc ggatactcgg gagcaactaa
     1801 atacggaaaa tattttattt catttccgtt taatccataa catgttcccg attgtaaaat
     1861 attatgttta tatacatctc tccgttttaa ttgtcctccg gcaaaataac acaacacttc
     1921 accatgcaat aatccactat cgtaatgagc tatagattta atttttccat tctcatgata
     1981 tgatttacat tctccatgtt ttatacccaa cacataattt gaatagtaac attccgagac
     2041 aatattatct tttagattag aaattacaac agaagcttta ctcaaaaccg tatcagaata
     2101 gtaaatagtt gcaatatatt tagcaacact actatcacac acatcaaaat attcattcaa
     2161 atactgtttt gaaacttttt gtacctgagc ttgtagcata aaactcaaac tacaaaatac
     2221 gcacaatact atatatctca tacatttcaa cttactgtaa gcaaaataac aaaaaaaaag
     2281 ggaatcatag tatgactccc ctttttttta tgattttttc atattaacgt aacagtacta
     2341 ccgaaccaaa tttcgtttct gttccattcg gttttttcaa acctgtccat tcatttccgc
     2401 caacataatt agcttctatt ttccaaatat aagtatcaga ttgcagcatt tcaccattgt
     2461 atgttccatc ccattttcct acaaatattc catcttctac ttcgtctgaa taccatatta
     2521 aattacccca tttatcaaaa atccacacct cacatttttc aactccgtac gttacaggtt
     2581 ggaaaactct aactgctgct gccggatttg ttggagcaaa agctgttggc acataaatat
     2641 tacctaacac atcaatatac acttctttag aaactttagc tttacaacca taactatttt
     2701 ctacagttaa tattggatat ttaggtccta ctacataacc acctactgtt acaggttttt
     2761 ttctatcttt atagtctact actaaatcaa ccaaatcatc atcttttgtt tttgcaaaat
     2821 tccagtacat ggtgtatgtt acaggatatt ctaccaccgt tccgtcagct aatttgtcgg
     2881 ttacattttt ttgagtagta ctatcaagat attctacaac atttgatgct cccggcatag
     2941 taaaggtaaa gttaggtaca ggatagtttg ctactttcac tattaatgta tcaaagcctt
     3001 cacaacctgc atatgtattt tcatacactg ttattgtatc tactccttga atatcccagt
     3061 ctatgtaacg aacagccgat gaatttgcat cttttgaata atttaatcta ttaccggtaa
     3121 cagaccatat atatgtactt gcaacacttg gcacagtttc tacagtatac attacttcta
     3181 cagtatttac acaagcaagc gtatctccta caatttttgt ttttgctgca ggtgcaactt
     3241 caaattgtct gtttttagca gtatcacttg tacattctaa cacatcgggt tttacaatat
     3301 ttctatcgta taaatcaaat gtatagaatc caattggtag ttct
//
LOCUS       DAOTOW010000178         3334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22535_length_3334_cov_3.352851, whole genome shotgun sequence.
ACCESSION   DAOTOW010000178 DAOTOW010000000
VERSION     DAOTOW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22535_length_3334_cov_3.352851"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="P5243_04180"
     CDS             complement(<1..720)
                     /locus_tag="P5243_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRS18680.1"
                     /translation="MQLNYVPNNKRITINKELCNSCKQCEADCTSLAIHIDSNTIDNS
                     CILCGHCVAICKQQAISIDMIPPKKIDAQTITPLQFKTLCAHTRTCRNYKSQVVPQHI
                     IDEIIANSSLYPSASNIRSIHITAITDTSIIEALNNAVAQRLLQLFSFYSNPFISIIL
                     KPFISSKELRKLSNYKNLFVKKMEKHGQNITYKAPVVFLFHAKKTISGMQQTDADIWA
                     TYTSLLCSTYGLGTCFNGFIVK"
     gene            complement(711..1613)
                     /locus_tag="P5243_04185"
     CDS             complement(711..1613)
                     /locus_tag="P5243_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRS18681.1"
                     /translation="MATILIAGGTGLIGTRLQQILSSYFTVKVLSRNKSICNNSTLFY
                     WNPDNLEISSLAFENVTCVINLSGENISNKRWSHSQKEKIQKSRILSTQTLAQGIQKY
                     GTSVTTYISSSAIGYYGYNSNKKMFEENDVPGNDFLAETCKIWEEEAMRIENLGIKTI
                     IIRTGIVISKHGGMLHKILKLIPFRMIPVFGKGTNIIPWIHIDDMCAIYKFAIENNSL
                     QGIYNAVANNCSQLEFIQTIQHAYKKKTVLFHIPIVLLKSILGEMATILITGNAISNT
                     KIIEAGFSFKYTNTIKALEKEICN"
     gene            complement(1633..2085)
                     /gene="smpB"
                     /locus_tag="P5243_04190"
     CDS             complement(1633..2085)
                     /gene="smpB"
                     /locus_tag="P5243_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRS18682.1"
                     /translation="MTQKQIYIKNKKAGFEFEIIQTFTAGIVLTGTEIKSLRLGKASI
                     TESYGYMHNGELWIKGMHISEYSFGSYNNHDPIRERKVLLHKRELEKIEEKLKDKGIT
                     IAVVSLYITSRGWAKVDIALARGKKLHDKREDIKLKDAKREMDRAMKI"
     gene            2134..3069
                     /gene="miaA"
                     /locus_tag="P5243_04195"
     CDS             2134..3069
                     /gene="miaA"
                     /locus_tag="P5243_04195"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRS18683.1"
                     /translation="MTEPIKLPVIVGPTASGKTKLAAQCAYYLGAEIISADSRQVYKG
                     MDIGTGKDIDDYVVNNYAIPYHLIDIVDAGYTYNLYEFKRDFISVFNSLFEKGKKAIV
                     CGGSGLYTEAIIKQYELHEVPQDIEFRKKCEQRSFEELIEELKLYKTLHNKTDIDTKK
                     RVIRALEIAKYEQTYGTQIQFQKLPYEPIVIGINIDRESRRQRILNRLQLRMQQGMTH
                     EVEHLLSQGVTPEKLMYYGLEYKYITLYVTGKITEKQMFDELYIAICQFAKRQMTWYR
                     GMERRGIQIHWINGNTSIENATQEAIEIINKSKTT"
BASE COUNT         1150 a          481 c          509 g         1194 t
ORIGIN      
        1 ttttactata aaaccattga aacatgtgcc taatccatag gtactacaaa gtaacgatgt
       61 atatgttgcc catatatcag catcagtttg ttgcataccg gatattgttt tttttgcatg
      121 aaaaagaaat acaaccgggg ctttgtaagt aatattttgt ccatgtttct ccattttttt
      181 tacaaataga ttcttgtaat tagatagttt gcgtaattct ttgcttgaaa taaaaggttt
      241 taatataatg gaaataaatg gattactgta aaatgaaaat aattgtaata atcgttgtgc
      301 tacagcattg tttaatgctt caataattga agtatctgta attgcagtta tgtgaataga
      361 acgaatattg cttgctgaag gatataatga actatttgca ataatctcat caattatatg
      421 ttgtggtaca acttgtgatt tataatttct acatgtacgt gtgtgtgcac ataacgtttt
      481 aaattgcaaa ggagttatag tttgagcatc aattttttta ggaggaatca tatcaatgct
      541 tattgcttgt tgtttgcaaa tagcaacaca atgaccacat aaaatacacg aattatcaat
      601 agtatttgaa tctatatgaa tagctaatga agtacaatca gcttcacatt gtttacatga
      661 attacaaagt tctttgttta tggtaattct tttgttattt gggacataat ttagttgcat
      721 atttcttttt ctaaagcctt tattgtatta gtatatttaa atgaaaaccc cgcttctata
      781 atttttgtat ttgatatggc atttccagta ataagaattg ttgccatttc acctaaaata
      841 cttttcaaaa gcacaatcgg tatatgaaaa agaacagttt ttttcttata agcatgctgt
      901 atagtttgta taaattctaa ttgactgcaa ttgtttgcaa cagcattata tataccttgt
      961 aacgaattat tttctattgc aaatttatat atagcacaca tgtcgtctat atgtatccaa
     1021 ggaataatat tggttccttt tccaaataca ggaatcatac gaaaaggtat taacttcaat
     1081 attttatgta acataccgcc atgttttgaa ataacaatac ctgtgcgaat aataattgtt
     1141 ttaataccta aattttctat tcgcatcgct tcttcttccc aaattttgca cgtttctgct
     1201 aagaaatcat ttccgggcac atcattctct tcaaacattt ttttattgga attatatcca
     1261 tagtaaccta tagctgaaga agatatatat gtagttacag atgtaccata tttttgaatt
     1321 ccttgagcta atgtttgagt tgataggatt cggctttttt gaattttttc tttttgagaa
     1381 tgagaccaac gtttgttact aatattttct ccgctcagat taattacaca agttacattt
     1441 tcaaatgcta gtgatgaaat ttctaaatta tcgggattcc aataaaatag tgttgaattg
     1501 ttacatatag acttgtttcg acttagtact ttaacagtaa aatatgaaga taaaatttgt
     1561 tgtaaacgag tacctattaa accggtgccg cccgctataa gaatagttgc catattattt
     1621 aagtttaatg tgttatattt tcatagctct atccatttct cttttggcat cttttaattt
     1681 tatatcttct cgtttatcat gtagtttttt cccacgagca agagctatat caacttttgc
     1741 ccaccctcga gaagtgatat aaaggctaac aacagctatt gttatccctt tgtccttgag
     1801 tttttcttca attttttcta attcgcgttt atgtaataat acttttcttt ctctaatagg
     1861 gtcatgatta ttataacttc caaatgagta ttcagaaata tgcattcctt ttatccatag
     1921 ttctccgtta tgcatatatc catacgattc ggtaatactt gcttttccta atcgtaacga
     1981 ttttatctca gtacctgtaa gaactatacc ggcagtaaaa gtttgaataa tttcaaattc
     2041 aaaacctgct tttttatttt ttatatatat ttgtttttgt gtcatgtatt tttaaaactg
     2101 ttttcaaaaa tacatatttt attgcaattt actatgacag aaccaataaa attacccgta
     2161 atcgtaggtc ctacagcaag cggaaaaaca aaacttgctg cacaatgtgc gtattattta
     2221 ggagcagaga ttataagtgc cgattccaga caagtatata aaggaatgga tataggaacg
     2281 ggtaaagata ttgatgatta tgttgttaat aattatgcaa ttccttatca tttgattgat
     2341 attgtagatg ccggttatac atataattta tatgaattta aacgagactt tatttctgtt
     2401 tttaattcat tatttgaaaa aggcaaaaaa gctatagtgt gtggcggttc agggttgtac
     2461 actgaagcta taattaaaca atatgaatta catgaggtgc ctcaagatat agaatttaga
     2521 aaaaaatgtg aacaacgctc atttgaagaa ttaatagaag aattgaaact atataaaaca
     2581 ttgcataata aaaccgatat tgacacaaaa aaacgagtta ttagagctct tgaaattgca
     2641 aaatatgaac aaacatatgg tacgcaaata caatttcaaa aattaccata tgaacctatt
     2701 gtaataggta ttaatataga tagagaatcc agaagacaac gaattttaaa tagattacaa
     2761 ttaagaatgc aacaaggcat gactcatgaa gtagaacatt tactttcaca aggtgttaca
     2821 cccgaaaaat taatgtatta tggactagaa tataaatata ttacattgta cgtaacagga
     2881 aaaattacag aaaaacagat gtttgatgaa ttgtatatag caatatgcca atttgcaaaa
     2941 cgtcaaatga cctggtatag aggtatggaa cgcagaggaa tacaaattca ttggattaat
     3001 ggaaatacta gtatagaaaa tgcaacacaa gaagctatag aaataattaa caaatcaaaa
     3061 acaacataac acaaaaaata ccggaaaaca aagggggggg gatttgctgt ttccggtatt
     3121 aaagagaatt atggaactat gaatatatac acagagggct ctgtgttttt ttatcttcta
     3181 cgatcgtaat aattgggagc tctataataa tcagtgtcat aatcataatc gcaaccacca
     3241 taacaatatc catgtcggta tacaactgtt tctctacaaa caggttctga tttagcatat
     3301 actttatggt taggtttata atacactatt tctt
//
LOCUS       DAOTOW010000179         3323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22637_length_3323_cov_3.350673, whole genome shotgun sequence.
ACCESSION   DAOTOW010000179 DAOTOW010000000
VERSION     DAOTOW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22637_length_3323_cov_3.350673"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5243_04200"
     CDS             <1..230
                     /locus_tag="P5243_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRS18684.1"
                     /translation="SKQKIEITPENFKGYTNVHSIIEDGWYKYYIGTYYSFKEISVPF
                     QQIKTKFPEAFIVARKDGKKITIQEAKSITQ"
     gene            245..1237
                     /locus_tag="P5243_04205"
     CDS             245..1237
                     /locus_tag="P5243_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRS18685.1"
                     /translation="MEKDKAIKIGLLLVIMFGILYWGFNFLKGKNIFTKEYTYYAVYE
                     RIDGLQKNSTIYLRGHKVGQVTDIQFTSKNYESITLTFAIDKSVQIPANTIARIFSSD
                     IMGTKSIDLIIPHFVQSQNVEILKDKSMLIPDIEGSLTDQVRLEMAPLKKQIEKLMES
                     TGTAIEQLKFVLNEKTGESLRTSFEKIQNAVDAMQHSSVTIDTILTNANQNIQKLLTN
                     IESITKNISTKNKEIDNIINNFSSISDTLAKANIAQTIYKTESVMTELSVMLDKVNKG
                     EGTLGAIMQNQNLYNNLESTSKQLDNLIRDIQKNPKRYVSFPIMNINKKEKTEQ"
     gene            1491..2930
                     /gene="dnaA"
                     /locus_tag="P5243_04210"
     CDS             1491..2930
                     /gene="dnaA"
                     /locus_tag="P5243_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRS18686.1"
                     /translation="MEVLHETLWSNCLEIIKDNLGSKLSFETWFKPIKPVKVENDILT
                     IQVPSPFYYEYIETHYIDLLSKALRKVLGANAKLEYKVIMEQPAHAETQPTAIQLPGK
                     NATNLKNKIFHQPTYVTDNNIIKNPFIIPGIKNLNIDSQLNPNYSFSNFVEGECNRLA
                     RAAGVTVAQNLGKTNYNPLFIYSKPGLGKSHLVQAIGIEIKEKYPKKAVLYVDADTFQ
                     RQFAEAAIKNERNDFMHFYQMIDVLIIDDVQNFTGKERTQKAFFQIFNYLHQKNKQII
                     LTSDCPPVELQGLEERLLSRFKWGLSAELKVPDFETRLEILKRKTYDDGIEIQDEILE
                     YIANHITTNIRELHGVLISILAQSTINNKQITLDLAKDIVEKLVKNTQQDISIDFIKK
                     TVCDYFNIGIDQLKSKTRKREIVQSRQVAMYFAKSYTKNSLASIGAQIGGKDHATVLH
                     ACKTVNNLMETDKRFKRYIQDIEKRFKLQ"
     gene            3052..>3323
                     /locus_tag="P5243_04215"
     CDS             3052..>3323
                     /locus_tag="P5243_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRS18687.1"
                     /translation="MKRTIIIVRHAKSDWSNGELSDFERPLNARGKRDIPIMASYLIQ
                     HYPSIDLAIASDSVRTTETIEGIKKYGYTIAQLSYNHKLYLASKET"
BASE COUNT         1385 a          435 c          461 g         1042 t
ORIGIN      
        1 cgtcaaaaca aaaaattgaa ataactcccg agaatttcaa aggatataca aatgttcata
       61 gtataattga agatggttgg tataaatatt atataggaac atattattct tttaaagaaa
      121 tttccgtacc tttccaacaa ataaaaacaa agtttccgga agcttttatt gttgcaagaa
      181 aagatggtaa aaaaatcaca atacaagaag caaaaagtat aacacaataa aaaatttaat
      241 acacgtggaa aaagataaag caattaaaat tggattactc cttgttataa tgtttggcat
      301 attatattgg ggatttaact ttttaaaagg gaaaaatatt ttcaccaaag aatatacata
      361 ttatgctgta tatgaacgaa tagacggatt acaaaaaaat agtaccattt atttacgagg
      421 tcataaagtt ggacaagtta ccgatattca atttacatca aaaaattatg aaagtataac
      481 gcttacgttt gcaatagata aatcggttca aataccggca aatacaattg cacgtatatt
      541 tagttctgat attatgggaa ctaaatcaat cgatttaatt attccacact ttgttcaatc
      601 acaaaatgta gagatactga aagataaatc aatgctaatt cccgatattg aaggaagttt
      661 aaccgatcag gtgagattag aaatggcacc gctcaaaaag caaatagaaa aacttatgga
      721 aagtaccggt actgcgattg aacaattaaa atttgtactt aatgaaaaaa ccggagaaag
      781 cttacgtact agttttgaaa aaatacaaaa tgctgttgat gcaatgcagc attcaagtgt
      841 tacaattgat actattttaa caaatgctaa tcaaaatatt caaaaattat taactaatat
      901 agaatctata accaagaaca ttagtacaaa aaataaagaa attgataata taatcaataa
      961 tttttcatca ataagtgaca ctttggcaaa agctaatatt gctcaaacca tatataaaac
     1021 agaaagtgta atgacagaat taagcgtaat gcttgataag gtaaataaag gagaaggaac
     1081 cttaggggct attatgcaaa atcaaaatct ttacaataat ttagaatcaa catcaaaaca
     1141 acttgataat ttaatacgcg atattcaaaa aaatccgaaa cgatatgtat catttccaat
     1201 aatgaatatc aataaaaaag aaaagacaga acaataactt taatcaaata ttacatacac
     1261 ttgattacaa atgttttata tgtaaagcat ttgtaatcag tatgttaaaa aaaatatttt
     1321 tttttttatt tttttttaat ttataagttg ttgatagtaa aaaccgtatg atttttttgt
     1381 aaaaaacaag agtaaaacga cttttttttt acaaatttta taagcaaatt tgtagaacga
     1441 taaagaacga aaatttcaac attaaataac tatagtataa taatagaata atggaagtat
     1501 tacacgaaac attatggtca aattgtttgg aaataattaa agacaacctc ggttccaaac
     1561 tatcttttga aacttggttt aaacctatta agcctgtaaa agtagaaaat gatatactta
     1621 ctatacaggt gccaagtcca ttctattatg aatatattga aacacattat atagatttat
     1681 taagtaaagc tcttagaaaa gttttaggtg caaatgctaa attagaatat aaagtaataa
     1741 tggagcaacc tgcacatgca gaaacacagc ctacagcaat tcagttaccc ggaaaaaatg
     1801 ctactaatct taaaaataaa atttttcacc aaccaacata tgttaccgat aataatataa
     1861 taaaaaatcc ttttattatt ccgggtatta aaaatctaaa tattgattct cagttaaatc
     1921 ctaattattc attttcaaat tttgttgaag gagaatgtaa tagattagct cgtgctgccg
     1981 gggttactgt tgctcaaaat ttaggaaaaa caaattataa tcctttattt atttatagca
     2041 aaccgggatt aggtaaatct catttagttc aagctatagg tatagaaatt aaagaaaaat
     2101 atcctaaaaa agctgtttta tatgtagatg ccgatacgtt tcaacgtcaa tttgccgaag
     2161 ctgcaattaa aaatgaacgt aatgatttta tgcattttta tcaaatgata gatgttttaa
     2221 taattgacga tgttcaaaat tttaccggaa aagaacgtac tcaaaaagca ttttttcaaa
     2281 tatttaatta tttacatcaa aaaaataaac aaataattct tacatcagat tgtccacctg
     2341 tagaattgca aggattagaa gagcgcttgc tttctcgttt taaatgggga ctgtcggcag
     2401 aattaaaagt acccgatttt gaaactcgtt tagaaatatt gaaacgaaaa acatatgacg
     2461 atggtattga aattcaagat gaaatattag aatatatagc aaatcatata actacaaata
     2521 ttcgcgaatt acatggtgtg ttaatttcaa ttttagctca atctacaata aacaataaac
     2581 aaattacatt agatttagct aaagatattg ttgaaaaact tgtgaaaaac acacaacaag
     2641 atatatcaat agattttatt aagaaaacgg tttgtgatta tttcaatata ggtatagatc
     2701 aattaaaatc taaaacaaga aaacgtgaaa ttgttcaatc tcgtcaagtt gcaatgtatt
     2761 ttgcaaaaag ttatactaaa aattcattag cttctattgg tgctcaaatt ggtggaaaag
     2821 accacgctac tgttttacat gcctgcaaaa cagtaaataa tttaatggaa acagacaaac
     2881 gatttaaacg gtatattcaa gatattgaaa aaagatttaa attacaataa aacatacaaa
     2941 aggcgaatta cacattcgcc ttttttttgt tatatacaat aaaaaaaact catattcaag
     3001 tatttgtata taaaaaaaca ctatctttat agtttaatta aaaaaactat tatgaagcgt
     3061 acaatcatta ttgttcgtca tgcaaaatca gattggagta atggagaatt atctgatttt
     3121 gaaagaccac ttaatgccag aggaaaaaga gatattccaa ttatggcaag ttatcttatt
     3181 caacattatc cttcgataga tttagctatt gccagtgatt ctgtaaggac aaccgaaact
     3241 attgaaggaa ttaaaaaata tggatatact atagctcaac tttcatataa tcataaactt
     3301 tatttagcat caaaagaaac tat
//
LOCUS       DAOTOW010000180         3316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22710_length_3316_cov_3.823674, whole genome shotgun sequence.
ACCESSION   DAOTOW010000180 DAOTOW010000000
VERSION     DAOTOW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22710_length_3316_cov_3.823674"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="P5243_04220"
     CDS             complement(<1..1899)
                     /locus_tag="P5243_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRS18688.1"
                     /translation="MRIIVVTVGLLMLIVLAGIFFYKQSSQSLIENTYNHLETITEIK
                     KQQFVDWFDDEKYDAKIISKNPFLQECLFSYIENPNEKNKKRLELFLQNIENEHGLLG
                     IYVINDERKVLIETKISNEKTFCGKHAIRGDEDSVMHTVIHFEEESKTYAIDFLYPIQ
                     YKGNQYFIVYQNNVNSFIFPLVSLWNAENSTAETVIIQKEADSVFVLNIRKYSQDSIP
                     YKVPMFKTYRALVQAAMGKTGRIQAVDYRNKNILAYISKIPGTDWAIMSKMDTDDVYA
                     SIHKNAGFIILFIILIIAFIVFAFAFIYANKQRKMFKSLYRMQRQFKTTLYSIGDGVI
                     TTDSKGRVQYMNPIAEELTGWKESEAQGKQIEEVFEIIHEYTRAAVENPVHKVLQQCS
                     IVGLANHTLLVHKITRKEIPIADSGAPILTNDNELQGVVLVFRDQSEEYARKKQLEQT
                     NQTLTTLISNLPGITYSCKNCSSWDMMYISDACSQITGYSKEDFIENHVHFRDIIHPD
                     DRQMVFDVIQNSIAQHQKFRLEYRILSKNNDVVYVWEQGQGVYSESHELLSVDGYIVD
                     ITHIKLNEIELASSKQLLHTILDTIPVSVFWKNKDLQYAGANINFLRDTNTTLQDIYG
                     KTDFELPWA"
     gene            complement(2036..2728)
                     /locus_tag="P5243_04225"
     CDS             complement(2036..2728)
                     /locus_tag="P5243_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRS18689.1"
                     /translation="MVSIFTRWIELLFPETCVVCGNTLRYSEKQLCTHCLSDIPQVYI
                     HNKNHNPLETLLWGQIPFVSATSFMLYTKKNKYASILHTIKYKKNKDLGIFIGEMFGK
                     ELVKSGFLHNVDAIVPIPLHKKREQERGFNQSTVIAQGISNISQIPVIDTAVMRIRYT
                     ESQTKKNKEERLRNVANIFEVTNYHVIQNKNILLLDDVITTGATCISCAETILEQGFI
                     GTLRIASVALAT"
     gene            complement(2728..>3316)
                     /locus_tag="P5243_04230"
     CDS             complement(2728..>3316)
                     /locus_tag="P5243_04230"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRS18690.1"
                     /translation="EECAVVTAIEHDKENVHFILTCSFVHELQIDQSIAHNGVCLTVV
                     KKTETSYTVTAIQETLLKSNLGTLTVGSKVNVERSMSMNGRLDGHIVQGHVDQTAICT
                     KIQEAEGSWYYTFEYEPLQQDYITVEKGSITVNGVSLTVVNSQPKSFQVAIIPYTYHH
                     TNFHTFIVGSIVNIEFDILGKYIAKYLHNMGIVQK"
BASE COUNT         1065 a          563 c          456 g         1232 t
ORIGIN      
        1 tgcccatggt aattcaaaat ctgttttacc atatatgtct tgcaatgttg tatttgtatc
       61 gcgtaaaaaa ttaatatttg ctcctgcata ttgcaaatct ttatttttcc aaaaaacact
      121 tactggtata gtatctaaaa tagtatgaag aagctgtttt gacgatgcca attctatttc
      181 attgagttta atatgtgtta tatcaactat atatccatct acagaaagca attcatgtga
      241 ttcagaataa actccttgac cttgttccca cacatataca acatcattgt tttttgaaag
      301 tattctgtac tctaaacgaa atttttggtg ttgagcaata ctattttgaa taacatcgaa
      361 taccatttgt ctgtcgtcag gatgaatgat atctctaaaa tgtacatgat tctctataaa
      421 atcttcttta gaatatccgg taatttgact acatgcatca cttatataca tcatgtccca
      481 cgatgaacaa tttttacacg aatatgttat gccgggtaaa tttgaaatta acgtagtgag
      541 agtttgatta gtttgttcta attgtttttt acgggcatat tcttcacttt ggtctctaaa
      601 taccaaaact accccttgca attcgttatc attagttaat ataggcgctc cgctatcggc
      661 aattggtatt tctttgcgag ttattttatg aactaataaa gtgtgattag ctaaaccaac
      721 aatgctgcac tgttgcaata ctttgtgaac aggattttca accgcagcac gagtatattc
      781 atgtattatt tcaaatactt cttctatttg tttaccttgt gcctccgatt ctttccaccc
      841 ggtaagctct tcagcaatag gattcatata ttgcacacgt cctttggagt cagtagtaat
      901 tacaccatcg cctatactat acaaagtagt tttaaactgc cgctgcattc tatagaggct
      961 tttaaacatt ttccgttgct tattggcata aataaaagca aatgcaaata ctataaaagc
     1021 tattatcaat ataataaata atataataaa acctgcattt ttgtgaatag aagcatacac
     1081 atcatcggta tccattttag acataattgc ccaatctgtt ccgggaattt ttgaaatata
     1141 tgccaatata tttttatttc tgtaatcaac tgcttgaatt ctacctgttt tccccatagc
     1201 agcttgtacc aaagctctat atgttttgaa cataggtact ttgtatggaa tactatcttg
     1261 tgaatatttt cgaatattca atacaaatac actatcagct tctttttgaa ttatgacagt
     1321 ttctgctgtt gagttttcgg cattccataa tgaaactaac ggaaaaataa aagaatttac
     1381 attgttttga tatactataa aatattgatt tcctttgtat tgaataggat ataaaaaatc
     1441 aatggcatat gtttttgatt cttcttcaaa atgaattact gtatgcatta cactatcttc
     1501 atcacctcgt atagcatgtt taccacaaaa tgttttttca tttgaaattt tagtttctat
     1561 aagaactttg cgttcgtcat ttattacata tatgcccaat aatccatgtt cattctcaat
     1621 attttgtaaa aataattcaa gtcttttttt atttttttca ttgggatttt caatatatga
     1681 aaataagcat tcttgtaaaa atgggttttt acttataatt ttagcatcat atttttcatc
     1741 atcaaaccaa tcaacaaact gttgtttttt aatttcagta atagtttcca aatgattata
     1801 tgtgttttca ataagcgatt gtgatgattg tttgtaaaaa aatattcctg ccaatactat
     1861 aagcattagc aatcctacag taacaacaat tattctcaca gtaaatatat acttagattt
     1921 agttgccata gaaaatactc aaaaaatatg aactataaaa gtacgttatt ttatagggta
     1981 aaaacaactt tttaaacata aaatttatat actaaaatac gtatttatac agaaattaag
     2041 ttgctaatgc aacacttgca atacgcaaag tgccaatgaa tccttgctct aggatggttt
     2101 cggcacatga aatacatgta gctccggtgg taattacatc gtcaagtaat agtatatttt
     2161 tattctgaat cacatgataa ttcgttactt caaatatatt agctacattg cgtagtcgtt
     2221 cttccttatt ttttttggtt tgactttcgg tatatcgtat tctcattaca gccgtatcta
     2281 ttaccggaat ttgtgatata ttagaaatac cttgtgctat aacggtactt tgattaaacc
     2341 ctcgttcttg ctcacgtttt ttgtgcaatg gtataggcac tatagcatca acattgtgca
     2401 aaaatcctga tttaacaagc tctttaccaa acatttctcc tataaaaatg cctaaatctt
     2461 tattcttttt atattttata gtatgtaaaa ttgaagcata tttatttttt ttggtataca
     2521 gcataaaaga agtagcactc acaaacggta tttgacccca caaaagcgtt tctaaaggat
     2581 tatgattttt gttgtgaata tacacctgcg gtatatcact taaacagtgt gtacataatt
     2641 gtttttcact gtatcgcaaa gtattaccac acacaacaca agtttcggga aacagaagtt
     2701 caatccagcg tgtaaaaatt gacaccatta tttttgtact atacccatgt tgtgtaaata
     2761 ttttgcaata tacttaccaa gtatatcaaa ttcaatattt acaatagaac ctactataaa
     2821 tgtatgaaaa ttggtatgat gataggtata aggtataata gcaacttgaa acgatttagg
     2881 ttgagaattt acaaccgtta aactaacacc atttaccgta attgaacctt tttctacggt
     2941 tatataatct tgttgcaatg gttcatactc aaaagtataa taccaactgc cttcagcttc
     3001 ttggattttg gtacaaatag ctgtttggtc tacatgacct tgaactatat gcccatcaag
     3061 tctaccgttc attgacatac ttcgttcaac atttacttta ctccctactg taagagttcc
     3121 taaatttgac ttgagtaacg tttcttgaat tgctgtaaca gtataagagg tttccgtttt
     3181 ttttacaact gttaaacata ctccattatg agctatactc tgatctattt gcaattcgtg
     3241 tacaaacgaa catgtaagaa taaaatgtac attttcttta tcgtgctcta ttgccgtaac
     3301 aacagcacat tcttct
//
LOCUS       DAOTOW010000181         3315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22724_length_3315_cov_3.242638, whole genome shotgun sequence.
ACCESSION   DAOTOW010000181 DAOTOW010000000
VERSION     DAOTOW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22724_length_3315_cov_3.242638"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="P5243_04235"
     CDS             <1..1408
                     /locus_tag="P5243_04235"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRS18691.1"
                     /translation="TINTGSKLGLTITASHNPAKYNGYKAYWNDGCQLVAPHDTNVID
                     EVNKIASIDDVKWSGNEHNIILWDEEVDKPYVEAIKGLSLQPEIIQKQKDLKIVYTSI
                     HGTGITLVPRVLKEFGFTNVHIVEEQAEISGEFPSLRKPGTSAVREQDYTETEWLNKI
                     HEITGSPNPEKESAMKLAIEKGKALQADIIMATDPDGDRVGIVVKNAQGEYVILNGNQ
                     TGALLVQYLIQGWKAKGKLQGKEFVVKTIVTTELITEICKAYGVEMFDVLTGFKYIGE
                     KILELEGKKQFIGGGEESFGYLAGDFVRDKDAVIACAMIAEAAAVAKNEGKSLYDVLV
                     DTYVTYGLYKEDLTSIEKEGKEGKEAIAAMMQNYRTNPPAELNGSPVICIKDYEKQVE
                     TDCVANSQKPIQLPKSNVLQFFAQDGSKVTVRPSGTEPLIKFYFGVKTEISGVESMAK
                     AEEILQSKISGLKKSLGI"
     gene            1504..2511
                     /gene="pta"
                     /locus_tag="P5243_04240"
     CDS             1504..2511
                     /gene="pta"
                     /locus_tag="P5243_04240"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRS18692.1"
                     /translation="MDFVKYFREQAKSNPQRIVLPEGTEPRTLRAADYIIKEGIAKLI
                     IIGKRDFIEDFARKEGLTYLPQAEIIDPLNHPKMEEYAQMVVEIRKNKGIEIETAREL
                     IKNPLYLAATIIKAGDADGEVAGAENATQNVLRPGFQLIKTLPGVSLVSGVFIMILKD
                     KEFGDDGVMLFADCAVNPDPTEDELAEIAVTTAKTAQNLLGMTPRIAMLSFSTHGSAK
                     HKMIDKVAHATHIAQTNYPNLIIDGEMQLDAAIIPEVCLKKSPNSKIKGNANVLIFPT
                     LESGNIAYKLVHRFAHADAYGPILQGLAAPINDLSRGCSVEDIINVVAITVIQAQNRK
                     K"
     gene            2524..>3315
                     /locus_tag="P5243_04245"
     CDS             2524..>3315
                     /locus_tag="P5243_04245"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRS18693.1"
                     /translation="MKILVLNCGSSSIKYQLLQMNQEEVIAQGMIDRIGEEKSKVKLE
                     LANGEKHTTELHIPNHDQGVQYIIDDLCNPQHNIITNKHEIVAVGHRVVHGAEVFSKS
                     TKLTAEVIAQLEKCCELAPLHNPANLKGIYAAQKMLPHAIQCGTFDTAFHQSMPAHAF
                     MYGLPYEYYERLKVRRYGFHGSSHRYVSQQASKLAGKSYDACKTIVCHLGNGSSISAV
                     LNGKSVDTSMGLTPLEGLIMGTRCGDLDLGAALYIMRTDNLSVDEF"
BASE COUNT         1227 a          484 c          659 g          945 t
ORIGIN      
        1 gacaataaac actggatcaa aactaggcct gactattact gcttcgcata atcctgcaaa
       61 atataatggt tacaaagcat attggaacga tggctgccaa ttggtagcac ctcatgatac
      121 caatgtaata gatgaggtaa ataaaatagc aagtattgat gatgtaaaat ggagtggcaa
      181 tgaacataat ataatattat gggatgaaga agttgataag ccatatgtag aagctattaa
      241 agggttatct ttacaacccg aaattattca aaagcaaaaa gatctgaaaa tagtatatac
      301 ctcaatacat ggaacaggaa taactcttgt tcctcgtgtt cttaaagaat ttgggtttac
      361 taatgttcat atagttgaag aacaagcaga aatttcagga gaatttccat cgttacgaaa
      421 acctggcaca tctgctgtta gggagcaaga ttatacagaa accgagtggc tcaataaaat
      481 acatgaaata accggttctc caaatcctga aaaagaatcg gctatgaaat tggcaataga
      541 aaaaggtaaa gctttgcaag ccgatattat aatggctact gatcctgatg gcgaccgagt
      601 tggtatagtt gtgaaaaacg ctcaaggaga gtatgtaatt ctcaacggaa atcaaacggg
      661 agcattactt gtacaatatt taattcaagg gtggaaagct aaaggaaaat tacaggggaa
      721 agaatttgta gtaaaaacaa ttgtaactac cgaattgatt acagaaattt gtaaagcata
      781 tggagttgaa atgtttgatg ttcttaccgg atttaaatat ataggcgaaa aaattcttga
      841 acttgaaggt aaaaaacaat ttataggtgg tggcgaagag agctttggtt atttggcagg
      901 tgattttgtt cgcgacaaag atgctgttat agcatgtgct atgattgcag aagctgctgc
      961 tgttgctaaa aatgaaggaa aaagtttata tgatgtgttg gtagatacgt atgttacata
     1021 cggattgtat aaagaagatt taacttctat agaaaaagaa ggaaaagaag gaaaagaagc
     1081 aattgcggct atgatgcaaa attatagaac caatcctcct gcagaattaa acggttcacc
     1141 ggtaatatgc ataaaagatt atgaaaaaca agttgaaaca gattgtgtag caaactctca
     1201 aaaaccaata caattaccaa aatcaaatgt gttgcaattt tttgcacaag atggttcaaa
     1261 agtaactgta cgtccatcgg gaacagaacc gcttattaaa ttttattttg gtgtaaaaac
     1321 tgaaatttca ggagtggaat caatggcaaa agcagaagaa atattacaat caaaaatttc
     1381 cggtttaaaa aaatcattag gaatataagt caaaaaaagc acttcaaaat agaagtgctt
     1441 tttttttgaa atatattttt tattcgtact tttatagaat taaatttaaa tataaaaaac
     1501 actatggatt ttgtaaaata ctttagagag caagccaaaa gcaatccaca acgaattgtg
     1561 ttgcccgaag gcacagagcc gcgaactctg agagcagcag attatataat aaaagaaggt
     1621 attgcaaaac taataattat tggtaaacgc gattttattg aggattttgc acgtaaagaa
     1681 ggattgacgt atttgcctca agcagaaatt atagacccgc tgaatcaccc taaaatggaa
     1741 gaatatgctc aaatggtggt tgagattcga aaaaacaaag gtatagaaat agaaacagca
     1801 cgagaactta ttaaaaatcc attgtatttg gctgctacta ttataaaagc aggtgatgcc
     1861 gatggtgaag ttgccggtgc tgaaaatgca actcaaaatg tactacgtcc cggatttcaa
     1921 ttaattaaaa cattgcccgg agtttcactt gtatcgggag tgtttattat gatactgaaa
     1981 gataaagaat ttggtgatga tggagtaatg ttgtttgccg attgtgctgt aaaccctgat
     2041 cctaccgaag atgaattagc agaaatagct gttacaacag caaaaacagc tcaaaatttg
     2101 cttggaatga ctccgcgtat tgctatgctt agttttagta cacatggcag tgcaaaacat
     2161 aaaatgatag ataaagtagc tcatgcaaca catattgccc aaactaatta tccaaattta
     2221 ataattgacg gagaaatgca acttgatgcc gcaattatac ccgaagtatg tttaaaaaaa
     2281 tcgccaaaca gtaaaattaa aggaaatgca aatgtgctta ttttcccaac attagaatca
     2341 ggaaatattg catataaact tgtgcatcgt tttgctcatg ccgatgcata tggccctatt
     2401 ttacaaggat tggcagcccc aattaatgac ttgtcgcgtg gttgttcggt tgaagatatt
     2461 ataaatgtgg tggcaattac cgtaattcaa gcacaaaata gaaaaaaata aacagttgaa
     2521 tacatgaaaa tccttgtttt aaattgtgga agttcttcaa ttaaatacca attgttgcaa
     2581 atgaaccaag aggaggttat agcgcaaggt atgattgata gaataggaga agaaaaaagt
     2641 aaagtaaaat tagagttggc aaatggagaa aagcatacta cagaacttca tataccaaat
     2701 cacgatcaag gagtacaata tattattgat gatttatgta atcctcaaca taatataatt
     2761 accaacaaac atgaaattgt tgctgttggg catagagttg tgcatggagc cgaagtgttt
     2821 agcaaaagta ctaaacttac tgctgaggtt atagctcaac ttgaaaaatg ttgtgagctt
     2881 gcaccattac ataatccagc aaatttaaaa ggaatttatg ctgcacaaaa aatgttgccg
     2941 catgctattc aatgtggaac ttttgataca gcctttcatc aatctatgcc tgcacatgct
     3001 tttatgtatg ggttaccata tgaatattat gaaagactca aagtacgtcg atacggattt
     3061 catggttcta gtcatcgcta tgtgagtcaa caagcttcaa aacttgccgg gaaatcatat
     3121 gatgcatgca aaacaattgt ttgtcattta ggcaatggtt cttcaatttc ggctgtatta
     3181 aacggtaaat ctgttgatac aagtatgggg ttaacaccat tagaaggtct tataatgggt
     3241 acacgatgtg gtgatttgga tttaggagcc gcattatata ttatgagaac agataatcta
     3301 tctgtagatg aattt
//
LOCUS       DAOTOW010000182         3315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22725_length_3315_cov_3.206442, whole genome shotgun sequence.
ACCESSION   DAOTOW010000182 DAOTOW010000000
VERSION     DAOTOW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22725_length_3315_cov_3.206442"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="P5243_04250"
     CDS             <1..866
                     /locus_tag="P5243_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRS18694.1"
                     /translation="AHTNATVKIYDKSWIEQQGMGGLLAVNKGSVYPAQFVHISWNPQ
                     NNTHKPIILVGKGIVFDTGGLSLKTPSTYMETMKADMGGAAAIVGALYAIIRQNIPLH
                     IHALIPITDNRPTGDAYAPGDVIRMHNGKTVEVLNTDAEGRMILADALSYATSLNPQL
                     VIDMATLTGAANAAVGEHVAVYMGTAIEYMPQFEQCALRVWEPIVQFPLWNLYAEQIK
                     SDIADCKNVGGQFAGAITAGKFLQQFTNYPWIHVDISGSAFLRTKWSYKGVGGTGFGV
                     RMLVEFLKTLQ"
     gene            906..2039
                     /locus_tag="P5243_04255"
     CDS             906..2039
                     /locus_tag="P5243_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS18695.1"
                     /translation="MYSKKNIVLLIAVFYLFIISCNQSKVIHNSSKSTLFRYAQNIRS
                     SKNRDTVIVYISQGNKTDSITYILCKNTSPKKNDITRKIFYVPIPVTHVAALSTTEIA
                     CIDAINQTESIIATSDIFRISNPTIQKKFKEGTILDLGTSMNENREKILIAKPDVVIK
                     TFFSPQDIQKDAILLQSGIPVIYINNWLEKSPLGRTEWIQFIGMLYGKEHEADSVFSV
                     IETQYRELSNKVKNLQNKPKVLAGDMIKDVWYLPGGASYMAQFIKDAGGDYIYANNTI
                     TGSSAVNIEQLIGYAKQADVWIGADCMTKQELLKKNNSYTYIPVCNSKRCYNYHKRRT
                     PQGGNDYWESGTIRADYILADLIHIFHPTILPEYTLVYFKNIE"
     gene            2050..3081
                     /locus_tag="P5243_04260"
     CDS             2050..3081
                     /locus_tag="P5243_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRS18696.1"
                     /translation="MQKTTTYLVILALCLPISFAADIVFGSVYIPLRDIVHTIFSSTT
                     QQNYAAIVLEIRLPRVIVACITGIALPISGLLMQTFFRNPLAGPDVLGISTGASLGVA
                     IFSLAGGVGSIAIITSTNILSSWGYILAAGIGSCIIMIIIAAITPKVRDTASLLIIGI
                     MFSSITSAIVSILQYFSNPQQIHEFLLWTFGSFSGLQKQQIWYMLYILIPILSYTFFI
                     QKSLNALLIGENYARSMGVNIPFLRISIVISASLLAGIVTAYTGPIGFVGMAVPHIAR
                     MIFKTANHKILLIGSALIGAHILMICDLISQLPGFDSSIPINIVTAFFGAPIVIMVIF
                     RGRKTRLHY"
     gene            3086..>3315
                     /locus_tag="P5243_04265"
     CDS             3086..>3315
                     /locus_tag="P5243_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS18697.1"
                     /translation="MTEHIFQDIVNAQDLTIGYRKKKGYITIAEHLNIHIYKGEMVCL
                     LGPNGCGKSTLIRTLAGMQQPLQGYVFIEGGD"
BASE COUNT         1178 a          545 c          575 g         1017 t
ORIGIN      
        1 ttgcgcacac caatgcaact gtaaaaatat atgacaaatc atggatagaa caacaaggta
       61 tgggaggatt actcgctgta aataaaggta gtgtgtatcc ggcacaattt gttcatatat
      121 catggaatcc acaaaacaat acacataaac caatcatatt ggtaggtaaa ggaatcgtat
      181 ttgacacagg tggtttaagc ctcaaaactc catcaacata tatggaaaca atgaaagcag
      241 atatgggagg agcagcagca attgtaggag cactatatgc aataatacgt caaaacattc
      301 ctttacatat acatgctctc attccaatta ccgacaaccg accaaccggt gatgcatatg
      361 ctcccggtga cgtaattcgc atgcacaatg gcaaaacggt agaagtactc aatacagatg
      421 ctgaaggacg tatgattttg gccgatgctt tgagctatgc aacatcatta aatccgcaat
      481 tagtaataga tatggctact cttacaggag ctgctaatgc tgctgtaggt gaacatgttg
      541 ctgtgtatat gggaacagct attgaataca tgcctcaatt tgaacaatgt gctttacgag
      601 tatgggaacc tattgtacaa tttcctcttt ggaatttata tgcagaacaa ataaaatctg
      661 atatagccga ttgcaaaaac gtaggaggtc aatttgccgg agctattaca gcaggtaaat
      721 ttttacaaca atttacaaat tacccttgga tacatgtaga tatttctggt tcagcttttt
      781 taagaacaaa atggtcatac aaaggagttg gaggaacagg ttttggagta agaatgttag
      841 tagaatttct caaaacattg caataatact ttagatttaa ataatagaca tattttattt
      901 ttataatgta ttcaaaaaaa aatattgtat tactaattgc agttttctat ttatttataa
      961 taagctgtaa ccaatcaaaa gtaattcata attcaagcaa atcaacattg tttcgctatg
     1021 cacaaaacat tcgcagttct aaaaacagag atactgtaat agtatatata tcgcaaggta
     1081 ataaaacaga tagtataacc tatattttat gcaaaaatac ttcgccaaaa aaaaacgata
     1141 taactcgtaa aattttttat gtaccaatac ctgtaacaca cgttgccgct ttatctacta
     1201 cagaaattgc ttgtattgat gctataaatc aaactgaatc aataatagct acaagtgata
     1261 tttttagaat ttcaaatcct acaatccaaa aaaaattcaa agaaggaact atacttgatt
     1321 taggaacaag tatgaatgaa aaccgcgaaa aaattttgat tgccaaacct gatgttgtta
     1381 ttaaaacatt cttttcacca caagatattc aaaaagatgc aattttatta caaagtggta
     1441 ttccggtgat ttacataaac aactggctcg aaaaatcacc tttgggaaga accgaatgga
     1501 tacaatttat tggtatgtta tacggtaaag aacacgaagc tgatagtgta ttttctgtaa
     1561 ttgaaactca atatagagaa ttaagcaata aagtaaaaaa tttgcaaaac aaacccaaag
     1621 tacttgccgg cgacatgata aaagatgtat ggtatctacc cggaggtgct agttatatgg
     1681 cacagtttat aaaagatgcc ggaggagact atatttatgc taacaatact ataaccggaa
     1741 gctctgctgt taatatagaa caactcatag gatacgcaaa acaagccgat gtttggatag
     1801 gtgccgattg tatgacaaaa caagaattat tgaaaaaaaa taattcgtat acttatatcc
     1861 cggtttgcaa tagcaaaaga tgttataatt atcacaaacg gcgaactcca caaggcggta
     1921 acgattattg ggaaagtggt acaatacgag cagattacat tcttgctgat ttaatacaca
     1981 tttttcatcc aactatatta cccgaataca cattagtgta tttcaaaaat atagaataat
     2041 tttgcacata tgcaaaaaac aacaacatac ttagttatat tggcattatg cctacccata
     2101 agttttgctg ccgatatagt atttggcagt gtttacatac cattgcgtga tattgtgcac
     2161 acaatattta gttcaacaac acaacaaaat tatgcagcaa ttgtattaga aattcggtta
     2221 cctcgggtaa ttgtggcatg tattacaggt attgcattac ctatatcggg actattaatg
     2281 caaacatttt ttcgaaatcc attagcagga cctgatgtac ttggtataag cacaggagca
     2341 agtttaggtg ttgccatttt ttcgttagca ggtggagttg gcagtattgc tataattacg
     2401 tctaccaata ttttaagtag ttggggatat atacttgcgg ctggaatagg ttcatgtatt
     2461 atcatgatta ttattgctgc cattactcct aaagttcgcg atacagcatc actacttata
     2521 atagggataa tgtttagcag tattacatct gcaattgtaa gcatattaca atatttttcg
     2581 aatccacaac aaatacatga atttttattg tggacatttg gcagtttttc aggacttcaa
     2641 aaacaacaaa tatggtatat gctatatatt ttaataccta tattatcgta tacatttttt
     2701 attcaaaaat ctctaaacgc tttacttata ggcgaaaatt atgcacgcag tatgggagta
     2761 aacataccat ttcttcgcat tagtattgta atttccgcat cacttcttgc aggcatagtt
     2821 acagcatata caggtcctat agggtttgtt ggcatggctg taccacatat agccagaatg
     2881 atttttaaaa cagctaatca caaaatttta cttattggtt cagcactaat aggagctcat
     2941 atactcatga tatgcgattt aatttcgcaa cttcccggat ttgattcatc tattccaata
     3001 aatatagtaa cggcattctt tggagctcct atagtaataa tggtaatatt ccgcggaagg
     3061 aaaacacgtt tacattacta acactatgac agaacatatt tttcaagata tagtaaatgc
     3121 tcaggattta accatagggt ataggaaaaa aaaggggtat attactatag ccgagcatct
     3181 taatatacat atatacaaag gtgagatggt ttgtttactt ggtcctaatg gatgcggtaa
     3241 atctacactg atacgcacac ttgcaggcat gcaacaacca ttacaagggt atgtattcat
     3301 agaaggtggt gatat
//
LOCUS       DAOTOW010000183         3305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22801_length_3305_cov_3.207077, whole genome shotgun sequence.
ACCESSION   DAOTOW010000183 DAOTOW010000000
VERSION     DAOTOW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22801_length_3305_cov_3.207077"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="P5243_04270"
     CDS             complement(<1..233)
                     /locus_tag="P5243_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18698.1"
                     /translation="MIIRNDTSCVNREIRIYLLVVFALLSTTIFSAPRIASVSGNWNN
                     TNTWGGESVPSISDDVTINSGVTVFINVDNAVC"
     gene            545..745
                     /locus_tag="P5243_04275"
     CDS             545..745
                     /locus_tag="P5243_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18699.1"
                     /translation="MAISSSAERLRQAILKAIEDHKITRAEYDEIINIATEDGHIDPQ
                     EQALLKELQNMIEDKSVKLVAK"
     gene            907..980
                     /locus_tag="P5243_04280"
     tRNA            907..980
                     /locus_tag="P5243_04280"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:941..943,aa:Met,seq:cat)
     gene            complement(1222..1308)
                     /locus_tag="P5243_04285"
     tRNA            complement(1222..1308)
                     /locus_tag="P5243_04285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Ser,seq:gct)
     gene            complement(1373..1663)
                     /locus_tag="P5243_04290"
     CDS             complement(1373..1663)
                     /locus_tag="P5243_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRS18700.1"
                     /translation="MKVKNQIQEKELIEVEELIADNYTLILYNDDFNTFDHVINCLIQ
                     ICKHEQLQAEQCAYLVHYTGKTDIMHGLFEELQSYKHALLEQRLSVVIEKSN"
     gene            complement(1678..2334)
                     /locus_tag="P5243_04295"
     CDS             complement(1678..2334)
                     /locus_tag="P5243_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18701.1"
                     /translation="MKTKHVLSIFALFFAITVTSVFAQPEWNRVNYETSTVFTGYVKV
                     NGMNADVNDMVAIFVNDECRMVARIIMHNDSSYVSAVIHSAGIPEQAVIKYWDSHTNT
                     VHTLDTTVTVTNHGTVKRFPIEIKSEQIPEEPTDVVVVGNKLEVFPSPFTNTIQIESS
                     KAIKSVQIFNTIGSKMYSKEENAAMQVIIDGSKLSPGMYLVSIEFIDGTIQTKKVFKK
                     "
     gene            complement(2362..3219)
                     /locus_tag="P5243_04300"
     CDS             complement(2362..3219)
                     /locus_tag="P5243_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRS18702.1"
                     /translation="MKRKIYISIIIFFSVFMISSCEFLDDLTGANLDELTTEQVIQGL
                     KTALTIGTDSSATSLSAVNGYYGNKLLKIPLPDEAELVRQQVVTLVNKVPALASYFNL
                     DQKFENVVLSINRAAEEAAKEAKPIFVDAITGITIDQGWDILNGKNPLAQTKAEGFDS
                     TAATGYFQAVTFSPLQGLFAPKIDAQLNKNLGLGFSANQAWATLRNSINSALNTIESN
                     LILNTLYQNSGYTVNRISQESIGEFATEKALNGLFYKVGEEEKKIRKNPYMWAEDIIK
                     KVFGSVYVQ"
BASE COUNT         1134 a          536 c          443 g         1192 t
ORIGIN      
        1 tgacaaacag cattatcaac attaataaat acggttacac cactatttat agtaacatcg
       61 tcagaaattg aaggtacaga ttctcccccc catgtatttg tattattcca atttccactt
      121 actgatgcaa ttcgaggtgc agaaaaaatt gttgtactca acaaagcaaa aacaactaat
      181 aaatatattc ggatttctct gtttacacac gaagtatcgt tgcgaataat cattgttttc
      241 ataacattaa tatttagaag ttaaacatta atatttgcac tctgattacc agttcgataa
      301 taaaaagaac aaacgatatt ttgcgaatct aaaaataaaa atacatattt aaaatatgct
      361 taatcaagta tatacatgaa taattataaa cttttgtttt ttttattagc catcacaaat
      421 aagtataaat gcttatcata ttagtatatg ttataaaacg taactataaa atatttaata
      481 ccatattgaa ttatggtaca ataattttta tatatttgaa tatcaatttt taaatcatat
      541 aaatatggct atcagttcaa gtgcagaaag attaagacaa gctattttaa aagctataga
      601 agatcataaa attactcgtg cagaatatga tgaaattata aacattgcaa cagaagatgg
      661 gcatatagac ccccaagaac aagctctatt aaaagaatta caaaatatga tagaggataa
      721 atctgtaaaa ttggttgcaa aataaataac tattaatcaa tatattaata gcaatattta
      781 gattataaat ctgtgccact ttagtttttt tcaataaaaa accagtgttt tattgattta
      841 gtaaaaataa tttatatttt tgcacaccga aaaacaaaaa gttgttattc gatacaatat
      901 aataatggcg aggtagctca gttggttaga gcgcatgatt cataatcatg aggtcgcggg
      961 atcatgcccc gctctcgcta catatagctt gatagaaata tcaagctttt ttttctctat
     1021 tttatcatat gttactctca ttctttttat ttatcgttag agcgtcccga ttttttatcg
     1081 ggaaggttgt agtttctaaa atcttaaagt agcttactca caaatataat tttatagaat
     1141 gaaggaagtc aagtgtttta agcgtcctaa ttaattatca gagatggtaa aaaaaaagct
     1201 tacaatttct tgtaagctct gcggaaggag cgggattcga accctcggta cggttacccg
     1261 tacgacagtt tagcaaactg ttggtttcag ccactcaccc atccttccca gtttgtacta
     1321 aaaccgcagc aaaaatataa ctattattta aaaatgcaaa gattttgctt tgttaatttg
     1381 atttttctat tacaactgaa agtctttgtt ctaaaagagc atgtttatat gactgtaact
     1441 cttcaaacaa accatgcata atatctgttt tcccggtata gtgaacaaga taagcacatt
     1501 gctcagcttg caattgttca tgtttacaaa tctgtattaa acaattaatt acatgatcaa
     1561 atgtattaaa atcatcatta tacaaaatca atgtgtaatt gtcggcaatt aattcttcaa
     1621 cttcaattaa ttctttttcc tgtatctgat tttttacttt cattgtatta caaaaaacta
     1681 ttttttaaag accttcttcg tttgtatagt tccatcgata aactcaatag aaactaaata
     1741 cattccgggc gataatttac ttccgtcaat tattacctgc atagcagcat tttcttcttt
     1801 agaatacatt ttgcttccta ttgtattgaa aatctgaact gattttattg cttttgaact
     1861 ttctatttga attgtatttg taaacggaga agggaaaact tctaatttat tgcctacaac
     1921 tacaacatct gttggttctt caggaatctg ctctgattta atttcaattg ggaatcgttt
     1981 tactgtacca tgatttgtta cagtaacagt agtatctaaa gtatgaacag tatttgtatg
     2041 agaatcccaa tatttaataa cagcctgttc cggtattcct gctgaatgaa taacagcaga
     2101 cacatatgaa gagtcattat gcataatgat tcttgcaacc attctacatt catcgtttac
     2161 aaatattgca accatgtcat ttacgtcagc attcattcca ttaaccttca cataacctgt
     2221 aaatacagta gaagtttcat aatttacacg attccattct ggttgtgcaa atacacttgt
     2281 aacagtaatt gcaaaaaaca atgcaaaaat tgaaagtaca tgttttgttt tcatatctat
     2341 ttatttttaa aaattcataa tttattgaac atatacacta ccaaaaactt ttttaattat
     2401 atcttcggcc cacatatatg gattttttct aattttcttt tcttcttctc ctactttata
     2461 aaataaacca ttgagagctt tttcagttgc aaattcacct atactctcct gacttattct
     2521 gtttacggta taacctgaat tttgatacaa agtatttaat attagattac tttctatagt
     2581 gttaagagca ctatttattg aatttcgtaa tgttgcccat gcttgatttg cactgaatcc
     2641 taatcctaaa tttttattta attgagcatc aatttttggt gcaaataaac cttggagcgg
     2701 actaaaagtt acagcctgaa aataaccggt agctgctgtt gaatcaaaac cttctgcttt
     2761 agtttgtgcc aatggatttt taccatttag aatatcccat ccttgatcaa ttgtaatacc
     2821 tgtaatagca tctacaaata taggttttgc ttcttttgct gcctcttctg ctgctctatt
     2881 aatagataaa actacatttt caaacttttg atctaaatta aaatatgatg ctaatgcagg
     2941 cactttattt actaatgtta caacctgttg acgcaccaat tcggcttcat cggggagtgg
     3001 tatttttaac agtttatttc cataatatcc attaacagcc gacaaagatg tagctgaaga
     3061 atctgttcca attgtcaacg ctgttttcaa tccttgaatt acctgttctg tagttaattc
     3121 atcaagattt gcaccagtta aatcatctaa aaattcacat gatgaaatca tgaaaacaga
     3181 gaaaaaaatt ataattgaaa tataaatttt tcgtttcata tttaaaattt gtataaaatt
     3241 agtactatta tttcactgtt gtccgataaa aaattaagag aaggaagatg ttagtcttcc
     3301 tttct
//
LOCUS       DAOTOW010000184         3296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22880_length_3296_cov_3.106449, whole genome shotgun sequence.
ACCESSION   DAOTOW010000184 DAOTOW010000000
VERSION     DAOTOW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22880_length_3296_cov_3.106449"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..2662
                     /gene="clpB"
                     /locus_tag="P5243_04305"
     CDS             68..2662
                     /gene="clpB"
                     /locus_tag="P5243_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRS18703.1"
                     /translation="MDFNQFTIKAQEVINHSVQIAISKSHQAVETGHMLQALLEKAEN
                     ISSFLLKKLDVNTTMLSTVLHKIIDSYPKVSGGEPYLSSNANRALQESLQMLNKFNDR
                     YVSVEHILLGILKQGDQVSQLLKDNGVTEQNLILAIQDLRKGAKVESQSAEDTYNALQ
                     RFAINLNQQARNGKLDPVIGRDDEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIVNGDVPDTLKSKQIYSLDMGALIAGAKYKGEFEERLKSVVNEVISADGEIILFID
                     EIHTLVGAGKSDGAMDAANILKPALARGELRSIGATTLDEYQKYFEKDKALERRFQTV
                     MIDEPDVESAVSILRGLKERYENHHKVRILDEAIIAAVELSHRYITERYLPDKAIDLM
                     DEAASKLRLEMNSVPEEIDELNRKIRQLEIEREAIKREGLTAKVESLDKTIAELQSEH
                     ATFMAAWENEKRIVSNIQTIKQELEDLKYEAERAERDGDYGKVAEIRYGRIKERETQI
                     QQLQEELKEMQAKGGLIDEEVGADDIAEVVSRWTGIPVTRMLQAEREKLLNLEQELHK
                     RVIGQHEAITAIADAVRRSRAGLQDVKRPIGSFIFLGPTGVGKTELAKALAEFLFDDE
                     NLITRIDMSEYQERHSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGQLTDNKGRTASFKNTIVIMTSNIGSHIIQEHFDRMHESNVAETIE
                     RAKVEVFELLKRTVRPEFLNRIDEIIMFTPLSHSDILDIVRLQFNILAARLEKMNIHI
                     DITDDAVEELARLGYNPQFGARPVKRIIQKELLNQLSKKILTGEVSQDSRILIDFLGD
                     GMVFRNSK"
     gene            2674..>3296
                     /locus_tag="P5243_04310"
     CDS             2674..>3296
                     /locus_tag="P5243_04310"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRS18704.1"
                     /translation="MNRVVLITGASAGIGKETAKLLLQKGYVVYGAARRIEQMHDIAQ
                     LGAHILSLDITQDTSIQACIDTIISEQGRIDVLINNAGYGSYGAIEDVPIQEAKRQFE
                     VNVFGLARITQLVLPYMRNQKHGYIVNISSVAGKTYSPLGGWYHASKHALEGLSDSLR
                     FEVAPFGIHVIIIEPGLIQTEWGDIALHSAVQSSGNTVYASYVQGLQ"
BASE COUNT         1162 a          485 c          692 g          957 t
ORIGIN      
        1 tatgtaaaat tgtctttttt tttttgttgg caattgtttt gaagccgtaa aaaaaaaaaa
       61 tacgactatg gacttcaatc aatttacaat taaagctcag gaggttataa accattcggt
      121 acaaatagcc atttctaaat cgcatcaggc ggtagaaaca ggacatatgt tacaagcatt
      181 actggaaaaa gctgaaaata taagttcgtt tttactcaaa aaacttgatg taaatacaac
      241 aatgctgagt acagtattgc acaaaattat agattcttac cctaaagtat cgggtggaga
      301 accatattta tcatcaaatg ctaacagagc attgcaagaa tcattgcaaa tgcttaataa
      361 atttaatgat agatatgtat ctgtagaaca tattttgctc ggtattttaa aacaaggcga
      421 tcaagtatcg caattgctca aagataatgg tgtaaccgaa caaaatttaa ttcttgcaat
      481 acaagattta cgtaaaggag caaaggttga aagtcagtct gccgaagata cttataatgc
      541 attgcaacgt tttgcaatta atttgaatca acaagctcgt aatggtaagc ttgacccggt
      601 gattggtcgt gatgatgaaa ttcgtagagt tcttcaaatc ttatcacgaa gaactaaaaa
      661 taatccgatt ttaataggag aacccggtgt tggaaaaaca gctatagccg aaggattggc
      721 tcatagaatt gtaaatggtg atgtgcccga tacactcaaa tcaaaacaaa tttattcctt
      781 agatatggga gctcttatag ccggagctaa atataaagga gaatttgagg agcgtttaaa
      841 atcggttgta aatgaagtaa tttctgccga tggcgaaata atattgttta tagatgaaat
      901 tcatacatta gtaggtgcag gcaaaagtga tggagctatg gatgctgcaa atattttgaa
      961 accagccttg gctcgtggag aacttcgttc aataggtgct actactcttg acgaatatca
     1021 aaaatatttt gaaaaagaca aagcattgga acgaagattt caaaccgtaa tgatagatga
     1081 gcccgatgtg gaaagtgcag tttcaattct tcgcggactc aaagaacgat atgaaaatca
     1141 tcataaagta cgaattcttg atgaagcaat tatagctgca gtagaattat cgcatagata
     1201 tattaccgaa cgttatttgc ccgataaagc aatagatttg atggatgaag cagcttcaaa
     1261 acttcgtctc gaaatgaatt cggtacccga agaaatagac gaactgaatc gtaaaattcg
     1321 tcaacttgaa atagaacgtg aagccataaa acgtgaaggg ttaacagcaa aagttgagtc
     1381 gcttgataaa acaattgccg aattgcaaag cgaacatgct acgtttatgg ctgcatggga
     1441 aaacgaaaaa cgtattgtga gtaatattca aactataaaa caagaattag aagatttgaa
     1501 atatgaagca gaacgtgcag aacgtgacgg agattacggt aaagttgccg aaatacgata
     1561 tggtagaata aaagaacgcg aaacacaaat acaacaattg caagaagaac tcaaagaaat
     1621 gcaggcaaaa ggtggtttga tagatgaaga agtaggtgct gatgatatag ccgaagtagt
     1681 ttcgcgatgg acaggtattc ctgttacacg catgctacaa gccgagcgtg aaaagttgtt
     1741 gaatttagaa caagaattac ataagcgtgt tattggtcaa catgaagcta ttacagctat
     1801 agctgatgct gtgcgacgaa gtagagcagg tttgcaagat gtaaaacgac caataggttc
     1861 gtttatattt ttaggaccta ccggtgttgg aaaaacagaa ttagcaaaag cattggcaga
     1921 atttttattt gatgatgaaa atttaattac acgtattgat atgtctgaat atcaagaacg
     1981 tcattctgta tcgcgactta ttggagctcc tcccggatat gtagggtatg atgaaggagg
     2041 gcaattaacc gaagccgttc gtagaaaacc atattctgta gtattgctcg atgaaataga
     2101 aaaagctcat cctgatgtgt ttaatatatt attgcaagta cttgacgatg gacagttgac
     2161 tgataataag gggcgtacgg ctagttttaa aaatactata gttattatga cttcaaatat
     2221 tggttctcat attattcaag aacattttga cagaatgcac gaaagtaatg tggctgaaac
     2281 tatagaacgt gccaaagttg aagtgtttga attattgaaa cgaacagttc gtcccgaatt
     2341 tttgaacaga atagatgaaa taatcatgtt tactccattg agtcattctg atatattaga
     2401 tattgttcgt ttgcaattca atatattggc tgctcgttta gaaaaaatga atattcatat
     2461 agatataacc gatgatgctg ttgaagaact ggctcgactt ggatacaatc cgcagtttgg
     2521 tgctcgtccg gtaaaacgta ttattcaaaa ggaattactt aatcagttat ctaaaaaaat
     2581 attaaccggc gaagtttcac aagattcacg tatattaatt gactttttag gtgatggtat
     2641 ggtttttaga aatagtaaat agcggagtaa tatatgaata gagttgtctt aattacagga
     2701 gcttcggccg gtattggtaa agaaactgca aaattattgt tgcagaaagg atatgttgta
     2761 tatggtgcag cacgccgaat agaacaaatg cacgatattg ctcaattggg agcacatata
     2821 ctaagtttag atattacgca agatacttct attcaagcgt gtattgacac tattatttct
     2881 gaacaagggc gaatagatgt gttgataaac aatgccggtt atggttcgta tggtgctata
     2941 gaagatgtac caatacaaga agcaaagcga caatttgaag taaatgtatt tggactagct
     3001 cgaattacac aattagtgtt gccatatatg cgtaatcaaa aacacgggta tatagtaaat
     3061 atttcatcgg tagcaggtaa aacatattcg cctttgggag gttggtatca tgcttctaaa
     3121 catgccttgg aaggcttatc tgattcttta cggtttgaag ttgctccttt tggtatacat
     3181 gtaataataa tagaacccgg acttattcaa acagagtggg gagatatagc attacattca
     3241 gctgttcaat cgtcggggaa tactgtgtat gcgtcatatg tgcaaggatt acaaaa
//
LOCUS       DAOTOW010000185         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22892_length_3295_cov_3.610185, whole genome shotgun sequence.
ACCESSION   DAOTOW010000185 DAOTOW010000000
VERSION     DAOTOW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22892_length_3295_cov_3.610185"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="P5243_04315"
     CDS             complement(<1..486)
                     /locus_tag="P5243_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18705.1"
                     /translation="MKPRILYIVPHRFNRSPGQRFRCEHFIPILQNEGFDITYANLLS
                     EWDDIHFYKKNAYPQKLLILIKGFFKRLYHSIQARNYDIIFIYREAFMIGTTLFEHIL
                     HIWGKPIIYDFDDSIWLNDTSDGNKHLAWLKNTKKTNTILSLSHTVIVGNSYLAQYAK
                     QF"
     gene            complement(493..1497)
                     /gene="holA"
                     /locus_tag="P5243_04320"
     CDS             complement(493..1497)
                     /gene="holA"
                     /locus_tag="P5243_04320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRS18706.1"
                     /translation="MEYKQIIDDLSKKIYYPIYLLSGEEPYYIDQIADYIADTVLTPD
                     EQSFNQTIVYGKDSDVHTISQLARKFPMMSNYQVIIVKEAQDLKNIEEFEHYASNPMK
                     STILVICYKYKKYPKTTTFAKHVKKYGVIFDSEPLRDYQVSTWIQQYVKEKKYSINQD
                     TSELLAQYLGTSLHKIVNELQKLMIILPVGSTIGKSDIERNIGISKDYNTFELQDAFG
                     SRDNYKVQQIMKVFAANPKENPPSKIIPLLFTYFKNLLLIHFIPIKTNEAIVQHVKIS
                     NSSFIIKKYIDASKRYSPQKLVQIISLLREFDLKSKGVNSGNISEGDLLRELAFKILH
                     "
     gene            1551..2000
                     /locus_tag="P5243_04325"
     CDS             1551..2000
                     /locus_tag="P5243_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRS18707.1"
                     /translation="MQDLLLPKFDFSFKKINNTPYIFDEIRKKYVVITPEEWVRQHIV
                     KFLIQYKNYPASLMQLEVSMNIYGLQKRADIVCYNSQGMALLVVECKAPEVSINESVF
                     QQISRYNIALKAPYLMVSNGLKHYVCAIEFYSKSYTFLEDLPKFDTL"
     gene            2114..>3295
                     /locus_tag="P5243_04330"
     CDS             2114..>3295
                     /locus_tag="P5243_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS18708.1"
                     /translation="MKRKVCVLVLLCLWYQIFAQQEQATQVIRGKVIDAVTQYPLTGA
                     LVSLQGNTVLASLTNSYGVFEITGVPIGRQSIEVSYLGYKTKVIENVLVITGKQFVLD
                     IALEDDPIQIAQVVVQSNAHKTSLNEMAVVSSRAISVEQTERFAGSLGDPARMVANYA
                     GVSSKDDSRNDIVIRGNSPSGVLWRLNGIEIPNPNHFGALGATGGPVSMINNNLLTTS
                     DFMTGAFPAEYGNALAGAFDLYLRQGNTQKYEFTGQVGFNGFELGAEGPLTNKQTQNA
                     SYLINCRYSTLDVMHKLGVSMGTGAAIPQYKDVTYNVVVPTEKYGKFSFFGLWGNSFI
                     RLGRAAADTSENSYNARGTATDFGSGLGAYGLGHTYYINPKTRIRTTVSFQNMYVTTV
                     FD"
BASE COUNT         1104 a          442 c          564 g         1185 t
ORIGIN      
        1 gaattgttta gcatattgtg caaggtatga attaccaact attacggtat gtgatagaga
       61 aagaattgtg ttagtttttt ttgtgttttt gagccatgct aaatgtttat ttccatctga
      121 ggtatcattg agccatatag aatcatcaaa atcataaatt attggtttac cccatatatg
      181 aagaatgtgc tcaaataatg ttgtgcctat cataaatgct tcacgatata tgaaaattat
      241 atcgtagttg cgagcttgaa tgctatgata aagacgttta aaaaaacctt ttatcaagat
      301 tagtaatttt tgtggatatg catttttttt ataaaaatgt atatcgtccc attcacttaa
      361 taaatttgca tatgttatgt caaaaccttc attttgtaat ataggtataa agtgttcgca
      421 tcgaaatcgt tgacccggac tgcgattaaa tctatgtgga actatatata aaattcgggg
      481 tttcaataaa aatcaatgta atattttaaa tgctaattct ctcagcaaat cgccttcaga
      541 tatattacca ctatttactc cttttgattt taaatcaaat tctcttaaaa gcgaaataat
      601 ttgaacaagt ttttgaggac tatatcgttt tgatgcatct atatatttct taattataaa
      661 actggaattt gaaattttaa catgttgaac tatagcttca tttgttttaa taggaataaa
      721 atgaatgagc agtaaatttt taaaatatgt aaatagtaga ggaattatct ttgatggagg
      781 attttcttta gggtttgcag caaatacttt cataatttgt tgaaccttat aattatctcg
      841 tgaaccaaaa gcatcttgta attcaaatgt attgtaatct ttacttatac caatatttcg
      901 ttcaatatca gatttaccta tagtagaacc aaccggtaaa attatcatca atttttgtaa
      961 ttcatttaca attttatgaa gtgatgttcc taaatattga gcgagcaatt cactcgtatc
     1021 ttggttaatt gaatattttt tttcttttac atattgttga atccatgtac ttacttggta
     1081 atctctcaga ggttccgaat cgaaaatgac tccgtatttt tttacatgtt tggcaaatgt
     1141 tgtagtttta ggatattttt tgtatttata acaaattacc aaaatggtag atttcatggg
     1201 attacttgca taatgttcaa attcttcaat gttttttaaa tcttgtgctt cttttacaat
     1261 aataacttga taatttgaca tcataggaaa tttgcgagca agttggctta tagtatgaac
     1321 atcagaatct tttccataca ctatagtttg attaaatgat tgttcatcag gtgttaaaac
     1381 agtatcggca atataatctg caatttgatc aatgtaatat ggttcttcac ccgatagcag
     1441 ataaattgga taatatattt ttttggacaa atcgtctatt atttgtttgt attccataca
     1501 attgagttac aaaagatatt gtatttttgt ttttgtaaaa ataatacaaa atgcaagatt
     1561 tattgttacc aaaatttgat tttagtttca aaaaaattaa taacacccca tatatttttg
     1621 atgaaattag aaaaaagtat gtagtaataa cgcccgaaga atgggtacga caacatattg
     1681 ttaaattttt gatacagtat aaaaattatc ctgcttcgct catgcaatta gaagtttcaa
     1741 tgaacatata tggtttgcaa aaacgtgctg atattgtctg ttataattcg caaggtatgg
     1801 cattacttgt agttgaatgt aaagctcctg aagtatcaat taatgagagt gtttttcaac
     1861 aaatttcacg gtacaatata gctcttaaag caccatattt aatggtttca aatggactca
     1921 aacactatgt gtgtgctata gaattttata gcaaatcata tacattttta gaagatttac
     1981 ctaaatttga cacgctttaa agcgtaataa ttgcattcag catgtaaaag tcttcattca
     2041 acattttttt ttattttgtg cagattaaaa aagatagatt tgtttcaaag ttgtatttaa
     2101 aaatttgaaa aaaatgaaac gaaaagtatg tgtattagta ttactatgtt tatggtatca
     2161 aatatttgct caacaagagc aagctactca agtaattcgt ggtaaagtaa tagatgcggt
     2221 aactcaatat cccttaaccg gagcattagt ttctttacaa ggaaatactg tgttggcaag
     2281 tcttacaaat tcttatggag tatttgaaat tacaggtgtt cccattggcc gtcaaagtat
     2341 agaagtttcg tatttgggat ataaaactaa agttatagaa aatgtattgg taattacagg
     2401 taaacagttt gtattggata tagcactcga agatgatcct attcaaatag cgcaagtggt
     2461 tgttcaatca aatgcacata aaacaagttt aaatgaaatg gccgttgtaa gttcacgtgc
     2521 tatttcggtt gaacaaactg aacgatttgc cggaagttta ggagatccgg caagaatggt
     2581 tgctaattat gccggagtta gttcaaaaga tgattcacga aatgatattg ttattcgtgg
     2641 gaattcgcca agtggtgttt tatggcgttt aaatggtatt gaaataccta atccaaatca
     2701 ttttggtgct ttgggggcaa ccggaggacc tgtaagtatg attaataata acttacttac
     2761 tacatctgat tttatgacag gtgcctttcc tgccgaatat ggtaatgctt tggcaggagc
     2821 ttttgattta tatttacgac aagggaatac acagaagtat gaatttaccg gtcaagttgg
     2881 attcaatgga tttgaattag gagcagaagg accactgaca aataaacaaa cacaaaatgc
     2941 atcttattta attaattgta gatactctac attagatgtt atgcataaat taggggttag
     3001 tatgggtaca ggtgctgcta ttcctcagta taaagacgtt acgtataatg ttgttgtgcc
     3061 aaccgaaaaa tatggtaaat tttcattttt tggattatgg ggaaatagtt ttattcgctt
     3121 aggccgtgca gcagcagaca catcagaaaa tagctacaat gcacggggaa cagctaccga
     3181 tttcggttca ggattgggag catatggatt aggacataca tattatataa atcctaaaac
     3241 gcgaattaga actactgttt cttttcaaaa tatgtatgtt acaacagtat ttgat
//
LOCUS       DAOTOW010000186         3286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_22980_length_3286_cov_3.250696, whole genome shotgun sequence.
ACCESSION   DAOTOW010000186 DAOTOW010000000
VERSION     DAOTOW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22980_length_3286_cov_3.250696"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="P5243_04335"
     CDS             complement(<1..1429)
                     /locus_tag="P5243_04335"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS18709.1"
                     /translation="MQTNVYTVWGNCKDLLTEKKFLAYQHSEIQDRNRMALLVGGFTI
                     LLVGLSDFLRTNHQLFLTISITIRILFFLFCLFYFVTWKKKQTIQSIYTTSFIFSLFV
                     GISINAFIYLLNPDKIIEIIDLLTIPITFILVYVFVIIPVYLLLISGLVTSILYIIIL
                     SQFTNIDTSTIIILSIILVTIHAMGFYINRFLSKVRRNEFFRIQEIKILNIELHEEIE
                     ERKTVQEKLQKTYSEITESLYYASNLQVSLLPDLKITKKYISDYFVLYAPCDIVSGDF
                     YWATIYNDKVIVTAADCTGHGIRAGFMSFMAITLLDDIVHVKGITKASEILDNLREHV
                     IKNLHQTPENTDLRDGLDIALCVIDTENMVLEYAGAFNPLLHIRNHQMVEYKADRMPI
                     SFYIAAKPHFTNHTIPLEKGDKIYIYSDGYADQFGGEKNKKFTHKRLKREILISSKLP
                     MQEQYELLTYLHIQWKGKHKRTDDVL"
     gene            complement(1491..>3286)
                     /locus_tag="P5243_04340"
     CDS             complement(1491..>3286)
                     /locus_tag="P5243_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRS18710.1"
                     /translation="VSLGYPIKQTEAYSFFEIIIRIQQHISSGNINTKLLQSFCNHSY
                     SKIYLYESCNAIINQINNYTYSYIPLASVPIDDTIKHFIIKKTNTPYLQFLKNCITFI
                     ISNSHPNPIDSSIWYTIYTEIENLELTLQNSYIQSENITFLNSLLQQTISSKSVPIAG
                     EPLQGIQIMGILETRLLDFESITILSANEHILPKKSPGSSFIPYSLRVAFGMPTIKEH
                     NAIFAYYFYRIIQRANTVNILYSTQTNEQGTGEKSRYITQLMYELNQYNTKAHIQTSH
                     ISYPILPASNTILHYSKNTEEFSNFIEALHTEKRFLSPTSLYRYIECPLKFYFSVIAN
                     IRKPQEIKELPDNRDYGNFFHTAIENIYIPYINTLITSNTIDTILQNSHEIDTIVYNA
                     ISQVISNKNNVSIDENTVTLQKNVIKKYITNMLEFDKKQAPFTIIGLEHEIKHTFTIA
                     NTKIPIRGIIDRLQKNGNNTSILDYKTGKNKNTCETIKSIFDGTYATSNSAIFQICLY
                     AYIIQLQNPTETIEPHLVFLRDLHTNTTTNIYQKELKKHIQSYVEIHEIFEEYFNKTI
                     LSILDLQTPFTQTNDTKTCSYCDFKIFCKKQ"
BASE COUNT         1029 a          388 c          537 g         1332 t
ORIGIN      
        1 gtaatacatc gtctgtgcgt ttatgtttgc ctttccattg tatatgtagg taggttagta
       61 gttcgtattg ttcttgcata ggtaattttg aacttataag tatttctcgt ttaagacgtt
      121 tatgggtaaa ttttttgttt ttttcgcctc caaactgatc tgcatatcca tcggaatata
      181 tatatatttt atcgcctttt tctaaaggta ttgtatgatt tgtaaaatgt ggttttgctg
      241 ctatatagaa acttataggc attctgtctg ctttgtattc taccatttga tggtttctaa
      301 tgtgtaataa ggggttaaat gctcctgcat attctaacac catattctca gtatctatta
      361 cacataatgc aatgtctaat ccatctcgta aatcggtgtt ttccggagtt tgatgcaaat
      421 ttttgattac atgttccctt aaattatcta aaatttctga agctttagta attcctttta
      481 catgaactat atcatcgagt agggtaattg ccataaaact cataaaacct gctctaattc
      541 catgtccggt acaatcagct gcagtaacta taactttgtc gttgtaaatt gtagcccaat
      601 aaaagtcacc acttacaata tcacaaggag catataaaac aaaatagtct gatatatatt
      661 tttttgtaat ttttaaatca ggtaataacg atacttgtaa attacttgca taatataggc
      721 tttcggtaat ttctgaatat gttttttgta gtttttcttg tactgttttt cgttcttcta
      781 tttcttcgtg taattcaatg ttgagaattt taatttcttg aattctaaaa aattcatttc
      841 ttcgtacttt acttaaaaac ctgttgatat aaaaccccat agcatgtatg gtaacaagta
      901 ttatggatag gattattata gtagaagtgt caatgtttgt aaattgtgaa agtattatta
      961 tatacagtat cgatgtaaca agtccgctta taagtagcaa atacacaggt attattacaa
     1021 aaacatatac caagataaac gtaattggaa tagttagtaa atctattatt tcaataattt
     1081 tatcgggatt gagtaaataa ataaaagcat taatacttat tccaacaaac aagctaaata
     1141 taaatgatgt tgtgtatatt gattgtattg tttgcttttt tttccatgta acaaaataaa
     1201 acaagcagaa aagaaaaaaa agaattcgaa tagtaatact tattgtaaga aacaattgat
     1261 ggttggtgcg taaaaaatca cttaagccaa ccaacaatat agtaaatcct ccaaccaaca
     1321 gtgccattcg gtttctgtct tggatttctg aatgttgata ggcaagaaat tttttctctg
     1381 tgagtaaatc tttacaattt ccccatacag tgtatacatt cgtttgcatg tatagaaagt
     1441 attacaatgt cactaaaata gttattttta tttatttgtg caatgtaatt tcattgtttt
     1501 ttgcaaaata ttttaaaatc acaatatgag catgtttttg tatcgtttgt ttgtgtaaaa
     1561 ggagtctgta aatcaagtat tgaaaggata gttttgttaa aatattcttc aaatatttcg
     1621 tgtatttcta catatgattg tatatgtttt ttgagttctt tttgatagat atttgttgta
     1681 gtattagtat gtaagtctct taaaaatact aaatgtggtt caattgtttc tgtaggattt
     1741 tgcagttgaa taatgtatgc atataaacaa atttgaaata ttgctgaatt tgaggtagcg
     1801 taagtaccat caaatataga tttaatggtt tcacaagtat ttttattttt ccctgttttg
     1861 taatcgagta tagacgtatt gttaccattt ttttgtaaac gatctatgat gccgcgtata
     1921 ggtatttttg tgtttgctat tgtaaaagtg tgtttaattt catgttctaa accaataatg
     1981 gtaaatggtg cttgtttttt atcaaattca agcatgtttg taatatattt ttttataaca
     2041 tttttttgta atgtaacggt attttcatct attgatacat tgtttttatt ggaaattact
     2101 tgagatatag cattgtaaac aattgtatca atttcgtgcg aattttgaag tattgtgtct
     2161 attgtgtttg atgttataag agtgtttata tagggaatat atatgttttc aatagctgta
     2221 tgaaaaaaat tgccataatc tctattatca ggtaattctt taatttcttg tggtttgcga
     2281 atatttgcta ttacagaaaa ataaaatttg agcggacatt caatatatct ataaagcgaa
     2341 gttggtgaaa gaaatcgttt ttcagtatgt aatgcttcaa taaaattgga gaattcttct
     2401 gtgttttttg aatagtgaag aatggtgttt gatgccggta atataggata tgaaatatga
     2461 cttgtttgaa tgtgagcttt cgtattatat tggttcaatt catacattag ttgtgttata
     2521 tatctgcttt tttctccggt tccctgttca tttgtttgtg ttgaatacaa tatgttaacg
     2581 gtgtttgctc tttgaataat tctataaaaa taataagcaa atattgcatt atgttctttt
     2641 atggttggca ttccaaaagc aacgcgtaaa ctatatggta taaatgatga tcccggtgat
     2701 tttttaggta atatatgttc gtttgcagaa agtattgtga ttgattcaaa atcaagtaat
     2761 cgagtttcta atattcccat gatttgaatt ccttgcagtg gttctcctgc tataggaacc
     2821 gatttggaac tgatagtttg ttgtaataat gaatttaaaa atgtaatatt ttctgattgt
     2881 atatatgaat tttgtaaggt taattctaaa ttttctattt cagtatatat tgtataccaa
     2941 atacttgaat caattgggtt tgggtgtgag tttgaaatta taaatgttat acagtttttc
     3001 aaaaattgta agtaaggagt attggttttt tttattataa aatgtttgat tgtatcatct
     3061 attggaaccg atgctaatgg tatatacgaa tatgtgtaat tgtttatttg attgattata
     3121 gcattgcatg attcatacaa atatatttta ctatacgaat gattgcaaaa agattgcaat
     3181 agttttgtgt ttatattccc cgatgaaata tgttgttgta ttcgaataat aatttcgaaa
     3241 aaactgtaag cttctgtttg ttttatagga taccctaatg atacgt
//
LOCUS       DAOTOW010000187         3279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23042_length_3279_cov_3.308933, whole genome shotgun sequence.
ACCESSION   DAOTOW010000187 DAOTOW010000000
VERSION     DAOTOW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23042_length_3279_cov_3.308933"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..947)
                     /locus_tag="P5243_04345"
     CDS             complement(186..947)
                     /locus_tag="P5243_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HRS18711.1"
                     /translation="MKNVFLLITVIVGFVSVMVISSCNKEEVKPTILIDSVVDIDGNV
                     YKTIKIGKTWWMAENLRVTKFNNGTKLIHVTDKNDIDFPWQTLTKPAFCSYAFKQDTI
                     GLGYLYNWHVITDTNSIAPQGWRIATDEDWKQLEAYLGMSKSELDATGWRGENVGDKM
                     KAVGVTYWFPSTDKYNVWGEDVVGFKAMAGGCRLHNGKWGNPGIGYTGMWWTSTKTSD
                     TTALYRYLDYKQSKVFRAYAPLNYGMSIRCVKDDK"
     gene            complement(950..2299)
                     /locus_tag="P5243_04350"
     CDS             complement(950..2299)
                     /locus_tag="P5243_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18712.1"
                     /translation="MKTKQTKQTKQTKHIVQYIVALCVSVCGFSMLMPIHAQIENVIV
                     ETYYVSNAADATVTDGGSVPEGSVTYRVFIDLAKGSKLLGLFADEHHRLFIASDDFVY
                     NNTLDGVSIAKDLNKYRYSENTVALDSWLTLGQMSFSSSKTYGGVLKQNDTDGSKVGG
                     KNNDYNILINTDERAGIPITQADGMMIMSNVPDDSWIIYGLKDPLSGKDSTIFGSLKK
                     GKEFESYDFLLKCSGVQGLDSLNHVLVAQITTKGEIEFELNAEVETADGKIIKYVAVN
                     GGQLGSDIKLSPYLKYPQECGCTDRNYLEFDEKYSCSEPSACKTPIVLGCMDTYACNF
                     DATANVHIQNMCCYPGFCFDKDIQLVCPQFNAQPLVYMYPNPTKNNLTLKVLSPEETE
                     MSYKILDDMGSVQMQKENIKLGNFYTNSLQIGQLKSGIYHCVITIGNYTEFRTIIKE"
     gene            complement(2321..>3279)
                     /locus_tag="P5243_04355"
     CDS             complement(2321..>3279)
                     /locus_tag="P5243_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS18713.1"
                     /translation="VRYEDSYMFTDVNLYHELGLSPDDERRRTTLTQVNKPGKVNENK
                     LLPFATVIYKVQQETTTPLQLKLNYSKTLARPSIREISDISLFDFELRKQVSGNYILK
                     MVEIQNYDIRAEYYHPKGNSASVSLFAKNFKNHIELVDFDEIGYVWVNNLGKSWLRGV
                     ELEGTVTILKGLDFKSNVTLVQSQSTFSKSYTKNDGSYVYGDTVTRSMFGQAPYVING
                     ILTYTNDSLGATISLSYNLQGPRLVITGSGDIPDIYERERHLLDCKLSKKFGKHFSAS
                     FTVKDILNTAVVRTYRYNNAWLMDYDRYRYGTAYSVGLSYKL"
BASE COUNT         1071 a          614 c          416 g         1178 t
ORIGIN      
        1 tttacatccc attattcacg tcatctaata ttatatgaat gtaaaatcaa tatatattac
       61 aacaaaaaat atgatttttt gttgtaaaca ctatagataa tataattaaa aaagccacac
      121 atgtgtgtgg cttttttata atttatatgt aaatgcaaaa atctcgaaat ttgaagtaat
      181 cgaggttatt tatcatcttt tacacatcgt atactcattc cataatttaa tggagcatat
      241 gctctaaaca cttttgattg tttataatct aaatacctat acaacgcagt tgtatcagaa
      301 gttttggttg aagtccacca cataccggta tatccaattc ccggattacc ccatttacca
      361 ttatgtaatc tacatccacc tgccatagct ttaaacccga caacatcttc accccacaca
      421 ttgtatttat ccgttgatgg aaaccaatac gttacaccta cagctttcat tttatcgcca
      481 acattttcgc ctcgccaccc cgttgcatca agttcagatt ttgacattcc aagataggct
      541 tctaattgtt tccaatcttc atcagttgct attcgccaac cttgaggtgc aatagaattg
      601 gtatctgtta ttacatgcca attatataaa taccctaagc ctatagtatc ttgcttaaaa
      661 gcatatgaac aaaatgcagg ttttgtaagt gtttgccatg gaaaatcaat atcattttta
      721 tcggttacat gtattaattt tgttccatta ttaaactttg taacacgtaa attttctgcc
      781 atccaccatg ttttaccaat tttaatagtt ttatatacat taccgtctat atctactaca
      841 ctatcaataa gtattgtagg ttttacttct tccttgttgc acgaagaaat caccataaca
      901 cttacaaaac caactattac agtaattaat aaaaaaacgt ttttcattgt tattctttta
      961 taattgttct aaattctgta taattaccaa tagtaattac acaatgatat atacccgatt
     1021 ttaattgtcc tatttgcaat gagttagtat aaaaatttcc taattttata ttttcttttt
     1081 gcatttgtac cgaccccata tcatctagaa ttttatatga catttctgtt tcttcaggac
     1141 ttaatacttt aagagtcaaa ttatttttag ttggatttgg atacatataa accaaaggtt
     1201 gtgcattaaa ttgaggacat actaattgta tatctttatc aaaacaaaat ccaggataac
     1261 aacacatatt ttgaatatga acatttgctg tagcatcaaa attacatgca tatgtatcca
     1321 tacaacctaa cactatagga gttttacatg ccgaaggttc cgaacaagaa tatttttcat
     1381 caaattcaag ataatttcta tcggtacatc cacattcttg cggatacttc aaatatggac
     1441 ttagttttat atcagaacca agttgtccac catttacagc aacatattta ataattttac
     1501 catcagcggt ttcaacttct gcattcaatt caaattcaat ttcacctttt gtggttattt
     1561 gagcaactaa tacatgattt aaagaatcta aaccttgaac accactacat ttcaataaaa
     1621 aatcatatga ttcaaattct tttccttttt ttaatgaacc aaaaatagta gaatctttac
     1681 ccgacagcgg atcttttaat ccatatataa tccaactatc atccggtaca tttgacatta
     1741 tcatcatacc atcagcttgt gtaataggaa tgccggcacg ttcatcggta ttaattaaaa
     1801 tattgtaatc attattcttt ccaccaactt ttgaaccatc agtgtcattt tgttttaata
     1861 caccaccata ggttttagaa ctcgagaatg acatttgccc caatgttagc catgaatcga
     1921 gtgctacagt gttttcgctg taacgatatt tatttaaatc tttagcaata cttacaccat
     1981 ctaatgtatt attatacaca aagtcatcac tcgctataaa taaacgatga tgctcatcgg
     2041 caaataatcc caacaattta gaaccctttg ctaaatcaat aaatactctg tatgttacag
     2101 aaccttcggg aacactacca ccgtcggtaa cggtagcatc ggcagcatta gaaacataat
     2161 atgtttctac tattacattt tcaatttgtg catgaattgg cattagcata ctaaaaccac
     2221 atacacttac acataaagca actatatact gcactatgtg ttttgtttgt tttgtttgtt
     2281 ttgtttgttt tgttttcata tctctctgtt tttgtatttt ttataattta tatgataatc
     2341 ctacagaata tgcggttcca tagcggtatc tgtcataatc cataagccac gcattattgt
     2401 agcgatatgt tcttactact gctgtattca gaatatcttt tactgtaaaa cttgcagaaa
     2461 aatgttttcc gaacttttta gatagtttac aatcaagcaa atgacgttca cgttcataaa
     2521 tatcaggtat atcacccgat ccggtaatta ctaagcgtgg tccttgtaaa ttgtatgaca
     2581 aagaaattgt agcacctaaa ctatcattag tataggttaa tataccatta ataacgtaag
     2641 gagcttggcc aaacatactg cgtgttactg tatcgccata tacataactt ccatcgtttt
     2701 tagtataact tttactaaag gttgactgag actgaaccaa ggttacattt gatttaaaat
     2761 ctaacccttt aagtattgta acagtacctt ctaattctac tcctcgtaac cacgatttgc
     2821 caagattatt tacccatacg taacctattt catcaaaatc tactaattct atatgatttt
     2881 tgaaattttt agcaaacaaa cttaccgaag cactatttcc tttaggatga taatattcgg
     2941 cacgtatatc ataattttga atttcaacca tttttaagat ataattacca cttacctgtt
     3001 ttcgcaattc aaaatcgaac aatgaaatat ctgatatttc acgaattgat ggacgtgcaa
     3061 gtgtttttga ataatttaat tttaattgaa gcggtgtagt agtttcttgc tgcactttat
     3121 aaattacggt agcaaatggc aacaatttat tttcattaac tttaccgggt ttatttactt
     3181 gcgtaagagt agttctacgt cgctcatcat caggcgaaag tcctaattca tgatataaat
     3241 ttacatcggt aaacatataa gaatcttcat aacgcacac
//
LOCUS       DAOTOW010000188         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23108_length_3271_cov_3.510883, whole genome shotgun sequence.
ACCESSION   DAOTOW010000188 DAOTOW010000000
VERSION     DAOTOW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23108_length_3271_cov_3.510883"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="P5243_04360"
     CDS             <1..946
                     /locus_tag="P5243_04360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS18714.1"
                     /translation="LYLIIAIIILIIILLFSFLLYRQFTQKEKAYNLLSEKSNELEQA
                     YSNLEQLHAILKERNEKITDSLSYAKRIQKAILPQESIIEKAFARNFIYYLPKEIVSG
                     DFYWFTTQREYAFFAVVDCTGHGIPGAFMSMVGNTLLNQIVNEQHFTEPSEILQKLDK
                     EIIKTLKQYDEHTKQEDGMEISLIRYNTKTKQLVFAGAGHKIIVAYNNSIEIISSSIF
                     SIGGMHSIKKDNNVTFEQIDMKIIPGTTIYLFTDGYIDQFGGECDERFSTNRFQKLIA
                     DIQSYDLCEQYIHISKTFDDWKGATSQLDDVLVVGISL"
     gene            complement(948..1580)
                     /locus_tag="P5243_04365"
     CDS             complement(948..1580)
                     /locus_tag="P5243_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRS18715.1"
                     /translation="MTTSRKKSYPFIFSIITVIILIISYIFCVDTFTWFLEVFPIIIA
                     LPILFFTYNTFPLTRLLYILLIVHFIILAIGGIYTYAKVPLGFWIQDLFNQSRNNYDK
                     IGHFAQGFIPALLTRELLLRTSPLKQSKWLAFIIVSICLAISAMYEIIEWWTAELQGA
                     TAEAFLGTQGYEWDAQSDMFTALCGTIVALITLSKIHNKQLNTLYYKHQK"
     gene            complement(1590..2549)
                     /locus_tag="P5243_04370"
     CDS             complement(1590..2549)
                     /locus_tag="P5243_04370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS18716.1"
                     /translation="MAKGKYRFNAESLTYEKIEHSTKKIVMIGITYFVGFVTVGFIFM
                     FTWLHFFPSAREHALMDENAELRNQYSILDNRIKNLETLLEDMAERDNNIYRVIFEAN
                     PIPAEIRNAGFGGVNRYAELQSKPNMELVIETANRIDILNKKVYIQSLSFDTIISLAK
                     NKKDMLKRIPAIQPLRNYKFSSGFGYRIHPVYKTYRMHTGIDLTAPTGTKIYATGDGI
                     VREAEYSHGYGKMVLIDHGFSFQTVYGHMSKILVRPGQKVSRGDIIGMVGNTGTSTGS
                     HLHYEVRRHGNPVNPINYYLSDLSPKEFDELLQMSKQTNQSFD"
     gene            2736..>3271
                     /locus_tag="P5243_04375"
     CDS             2736..>3271
                     /locus_tag="P5243_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRS18717.1"
                     /translation="MNAQELRQSFLDFFASKQHTIVPSAPMVVKGDPTLMFTNAGMNQ
                     FKDIFLGNSPIVSPRIADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     SEAIAWAWEYLTNVLSINPECLYATVFQGDAADGTEFDKEAYDIWKQYLPEDRILMGN
                     KKDNFWEMGESGPCGPCS"
BASE COUNT         1126 a          488 c          550 g         1107 t
ORIGIN      
        1 attatactta ataattgcaa ttattattct tattataatt ttattgtttt cgtttttgtt
       61 gtatcgtcag tttactcaaa aagaaaaggc gtataattta ttatcagaaa agagtaatga
      121 attggaacaa gcatattcta atttagaaca actgcatgca attcttaagg agcgcaacga
      181 aaaaattacc gatagcttga gttatgcgaa acgaatacaa aaagctatat taccacaaga
      241 gtctattatt gaaaaagcgt ttgcgcgtaa ttttatatat tatctaccaa aagaaattgt
      301 aagtggtgat ttttattggt ttacaacaca acgcgaatat gcgttctttg ctgtagtaga
      361 ttgtaccggt catggtattc cgggagcgtt tatgtcaatg gtaggaaata cattactcaa
      421 tcaaattgtg aatgaacagc attttacaga accaagcgaa atattgcaaa aactagataa
      481 agaaatcata aaaactttga aacaatatga tgaacatacc aaacaagaag atggtatgga
      541 aattagttta attcgataca acaccaaaac caaacaatta gtttttgcag gagccggaca
      601 taaaattatt gtagcctaca ataattcaat agaaattatt tcaagtagta ttttttctat
      661 tggtggtatg cattcaataa aaaaagataa taatgtaaca tttgaacaaa ttgatatgaa
      721 aataataccc ggtacaacta tttatttatt taccgatgga tatattgatc aatttggagg
      781 agaatgtgac gaacgttttt caaccaatag atttcaaaaa ttaatagcag atattcaatc
      841 gtatgatttg tgtgaacaat acattcatat tagcaaaaca tttgatgatt ggaaaggtgc
      901 aacttcacaa ttagatgatg tgttggtagt tggaatttca ttgtaaatta cttttgatgt
      961 ttgtaataca atgtgttcaa ttgcttgttg tgaattttag ataatgttat aagtgctact
     1021 attgtaccgc ataatgctgt aaacatatcc gattgagcgt cccattcata tccctgagtt
     1081 cctaaaaacg cttcggcagt tgctccttgt aattcagctg tccaccattc aattatttca
     1141 tacatagcac taattgccaa acaaatacta acgataataa acgccaacca tttgctctgt
     1201 tttaatgggc tagttcgcaa caaaagttct cgtgtaagta gtgccggaat aaatccttgt
     1261 gcaaaatgac ctattttatc ataattgttt ctgctttgat tgaacaaatc ttgaatccaa
     1321 aaacctaatg gaactttggc atatgtatat atgccaccta ttgccagaat tataaaatga
     1381 actattagca atatatataa cagacgagtt aatggaaatg tgttatatgt aaaaaatagt
     1441 ataggtaatg ctatgatgat tggaaaaact tctaaaaacc aagtaaatgt atcaacacaa
     1501 aatatatatg agataatgag aatgattact gtaattatag aaaaaataaa aggatatgat
     1561 ttttttctgc ttgttgtcat aaactaatat taatcaaagg attggtttgt ttgtttagac
     1621 atttgcaata attcgtcgaa ttctttagga cttaaatcac ttaaatagta gttgattgga
     1681 tttaccggat taccatgacg acgaacttcg tagtgtaagt gagaaccggt agaagttccg
     1741 gtgtttccta ccatacctat aatatctccg cgagaaactt tttgccccgg tcttactaat
     1801 attttactca tatgaccata tacagtttga aaactgaatc cgtgatcaat aagaaccatt
     1861 ttaccatatc catggctgta ttctgcttcg cgaactatac catcaccggt ggcatatatt
     1921 ttagtaccgg ttggagctgt taagtcaatt ccggtatgca ttctatatgt tttatacacc
     1981 gggtgaattc tatatccaaa acctgatgaa aatttataat tcctaagtgg ttgaatagca
     2041 ggaatacgtt taagcatatc ttttttattt ttggcaagag aaataattgt gtcaaatgat
     2101 agagattgaa tatatacttt tttattaaga atatcaattc tattggcagt ttcaattacc
     2161 aattccatat ttggctttga ttgtaattca gcatatctgt ttactccgcc aaaaccggca
     2221 ttccgaatct cggcaggtat aggatttgct tcaaaaatga ctctataaat attattatcg
     2281 cgttctgcca tgtcttccaa caatgtttcc agatttttta tgcgattgtc taatatgctg
     2341 tattgatttc gtaattcggc attttcatcc attaaggcat gttcgcgagc cgaaggaaaa
     2401 aagtgtaacc acgtaaacat gaaaataaat ccaacagtaa caaatccaac aaaatatgta
     2461 atgcctatca ttacaatttt ttttgttgaa tgctcaattt tttcgtacgt taacgattcg
     2521 gcattaaatc tgtatttccc ttttgccata ttgatttttt agaaaatcgg atacaagtac
     2581 tattatattg tgtagttaca tataattttt gtaggtaacc aatttcttag tttcattttt
     2641 tataataagg tgcaaaaata cacaaaaaaa atatactttt gcacttttat agctaataag
     2701 tagaataagt attatataag tatttcaaaa tcaatatgaa cgctcaggaa ttacgacaat
     2761 cgtttttaga tttttttgca tcaaagcaac atacaattgt gccatcggct cctatggttg
     2821 taaaaggtga tccaacctta atgttcacca atgcaggaat gaatcaattt aaagatattt
     2881 ttttaggaaa ttctcctata gtatcgcctc gtatagccga ttctcaaaaa tgtttgcgag
     2941 tatccggtaa gcataatgat ttggaagaag tagggcatga cacatatcat catactatgt
     3001 ttgaaatgct cggaaactgg tcgtttgggg attattttaa atcagaagca atagcatggg
     3061 catgggaata tttaacaaac gtattgtcta taaaccctga atgtttatat gctactgtat
     3121 tccaaggtga tgctgctgat ggaacagagt ttgataaaga agcttatgat atatggaaac
     3181 aatatttacc tgaagataga attttgatgg gtaataaaaa agataatttc tgggaaatgg
     3241 gggaaagtgg tccttgtggt ccatgttcag a
//
LOCUS       DAOTOW010000189         3270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23116_length_3270_cov_3.348056, whole genome shotgun sequence.
ACCESSION   DAOTOW010000189 DAOTOW010000000
VERSION     DAOTOW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23116_length_3270_cov_3.348056"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="P5243_04380"
     CDS             <1..1733
                     /locus_tag="P5243_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS18718.1"
                     /translation="KDVTEEKIQFDASLLDANLTCEGLADSLGLSYLIGARYKNTSLI
                     LDSLDSQGEYKPIFYDIQALLKYSISPKLGVSYWLYGANNTYKFIPDTRTTDFGGLEK
                     QFRMNIYFEGNEQYTYQHIGNALTFHYQPNDRTKLDFTCMEYIGYEKETFDVLGQYRL
                     GEIIPDDKKPGAKSDSIKVLAVGSFLEHGRNSLYSKTFYTSHTGKRTGVFATYQWGIT
                     YSHNIYDAIYNEWTYIDSANYALPYSDTAITLKHVKNADLFLIQQTIAAYIQASKRID
                     FDNIWRSSLKIQTGLRTTYDTYTQSVLYNPRLRLLLKPKYDKNISISAASGVYYQPPQ
                     FKELIDENGNIYTDVLPQKSIQYVLGYTQGIHIWNRPFVFQTEAYYKQLQHIIPYLLQ
                     NVSTVYYPQLHAKGYASGIETKLNGEFVPGVESWISISSMKTMEWLKDSVSDIIRRPN
                     DQHINFAMFFQDYVPGSEYIKMSLTYLFGSRIPTAQPNATYNKFDDFTISPYSRVDLG
                     FLVILTGHKKQISKAIVKNMWLGAEVFNLFDNPNKISYFWIEDVNNTYYGVPNYLTSR
                     RFNIKLSVQL"
     gene            1730..>3270
                     /locus_tag="P5243_04385"
     CDS             1730..>3270
                     /locus_tag="P5243_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRS18719.1"
                     /translation="MSIAFKHPEFLYLLSAVVIPIIIHLFSFRKYKSVYFHSIFLLKN
                     IQQEQNKTRTKLKHLLILAARILTIISIVLAFAQPYIPNANYSQSQQRTHAVAIYIDN
                     SFSSQAQSKQGTTLDYMITKARELVSSYSSTQQFYCITNTPNQLEQIQLTKEQIQNKL
                     SEIKSSAFTKHISEVYASVQAIQKNIQLPVQLFLFSDFQKYATDFENILPDSTITIHI
                     IPFEQSTNNNIYIDSCWFTTPYRMRSQPEELVVRIENNSNQTYNNLPVKLFINDSLKS
                     IVPCSIEPFKKQDITVSFTINSPGIQALRIEIEDYPIVYDNTLYASYSIASQIPILLI
                     HEQKPNQFLLNLYKQSPYFTLQTQDVSRIDFSELPTYSVIILDGLQTISGGLAQVLQT
                     VIQSGKTVVCIPSARSNIDSYNSFFSKFGNQQFSKLDSTKTIANKVDFNHILYKHVFS
                     KTDKNTEYPTIFKHFPFTPIQHYTIIGLQNNNPLLTHIRFGKGSLFICSSPLTPQSNT
                     FISHP"
BASE COUNT         1216 a          605 c          414 g         1035 t
ORIGIN      
        1 ataaagatgt aaccgaagaa aaaattcaat ttgacgcaag tttattagat gctaatctaa
       61 catgtgaagg attagctgac tcattaggat taagctattt gattggagca cgatacaaaa
      121 acacctctct tattcttgat tcacttgact cgcaaggaga atacaaacca atattttatg
      181 atattcaagc tcttttaaaa tattcaattt ctccaaaatt aggagtttca tactggttat
      241 acggtgcaaa caacacatac aaatttatac cagatactcg tacaacagat tttggcggtt
      301 tagaaaaaca atttagaatg aatatatatt ttgaagggaa tgaacaatat acatatcaac
      361 atattggtaa tgctcttaca tttcactatc aaccaaatga ccgtacaaaa cttgacttta
      421 cgtgtatgga atatattggc tacgaaaaag aaacatttga tgtattaggc caatatagat
      481 tgggcgaaat aattcctgat gacaaaaaac ccggagcaaa atcagactct attaaagtac
      541 ttgcagttgg ttcattttta gaacatggtc gcaattcatt atattcaaaa acattttaca
      601 cttcacatac cggaaagcgt accggcgtat ttgctacata ccaatgggga attacatatt
      661 cgcataacat ttatgatgct atatataatg aatggacata catagattct gcaaattatg
      721 cattaccgta ttctgatacg gcaatcacat taaagcatgt taaaaatgca gatttgtttc
      781 ttattcaaca aactattgct gcatatatac aagcaagtaa acgaattgat tttgataata
      841 tatggagatc gtcactcaaa attcaaacag gattacgcac aacatatgat acgtatactc
      901 aatcagtatt atacaatcct cggttacggt tattactaaa accgaaatat gataaaaaca
      961 tttctatttc ggcagcatcg ggagtatatt atcaacctcc gcaattcaaa gaattaatag
     1021 atgaaaacgg gaatatatat accgatgtat tgcctcaaaa atcaatacaa tatgtactgg
     1081 gatatacgca agggatacat atatggaaca gaccatttgt atttcaaaca gaagcatatt
     1141 acaaacaact acaacatata attccttatt tattacaaaa cgtttcaact gtatattatc
     1201 ctcaactcca cgccaaaggc tatgcatccg gcatagaaac aaaattaaat ggtgaatttg
     1261 ttcctggtgt agaatcgtgg ataagtatat catctatgaa aactatggaa tggctcaaag
     1321 attcagtaag cgatattatt aggcgaccaa atgaccaaca tataaatttt gcgatgtttt
     1381 ttcaagatta tgtccccgga agtgaatata taaaaatgag cttaacatat ttattcggct
     1441 ctcgaattcc tacagctcaa cccaatgcca catacaataa atttgatgat tttactatta
     1501 gtccctattc tcgagttgat ttaggttttt tggtaatact tacaggtcac aaaaaacaaa
     1561 taagtaaagc tattgttaaa aatatgtggt taggtgccga agtatttaat ttgttcgata
     1621 atcccaataa aatttcatat ttttggatag aagatgtaaa caatacgtat tatggcgtcc
     1681 ccaattattt aacctcacgt agatttaaca taaaattatc ggtacaatta tgagtatagc
     1741 attcaaacat ccggaatttc tttatctttt aagtgctgtt gttataccta tcataatcca
     1801 tttattttcg ttccgcaaat acaaatctgt ctatttccat tctatatttt tactcaaaaa
     1861 tattcaacaa gaacaaaata aaacacgtac caaactcaaa catctattaa tattagcagc
     1921 tcgcatatta accattatct ctattgtttt agcatttgca caaccatata ttcctaacgc
     1981 taactattct caaagccaac aacgaactca tgctgtagct atttatattg acaattcatt
     2041 tagttcacaa gcccaatcaa aacaaggcac aacattagat tatatgataa caaaagcccg
     2101 tgaattagtg tcaagttatt cttctacaca acaattttat tgtattacca acacccccaa
     2161 ccaattagaa caaattcaac taactaaaga acaaatacaa aataaacttt ctgaaattaa
     2221 aagctctgca tttactaaac acatttcaga agtatatgct tcggtacaag caatacaaaa
     2281 aaatatacaa ttaccggttc aattatttct tttttctgac tttcaaaaat atgcaaccga
     2341 ttttgaaaat atactacccg attctacaat aactattcat ataatacctt ttgaacaatc
     2401 aacaaataat aatatttaca tagattcatg ctggtttacc acaccttatc gtatgcgctc
     2461 acaacccgaa gaattagtag ttcgcataga aaacaacagc aaccaaacat ataataattt
     2521 acctgtaaaa ttgttcataa atgactctct caaatcaata gtaccatgtt ccatagaacc
     2581 attcaaaaaa caagatatta cagtttcatt taccataaat tccccgggta ttcaagctct
     2641 tcgtatagaa atagaagatt accctatagt atatgacaat acgttatatg cttcatattc
     2701 catagcttct caaattccaa tactattgat acatgaacaa aaacccaatc aatttttact
     2761 caatttatat aaacaatcac cctattttac tttacaaaca caagatgttt cacgaattga
     2821 tttttctgaa ttacctacgt attcagttat tattttagat ggattacaaa ctatatccgg
     2881 aggattggct caagtactgc aaacagtaat tcaatcagga aaaacagtag tttgtatacc
     2941 atcagctcgc agcaatatag attcatataa ctcatttttt tcaaaatttg gcaatcaaca
     3001 gttttcgaaa cttgattcaa ccaaaactat tgcgaacaaa gttgacttta atcatatact
     3061 ctataaacat gtattttcaa aaactgataa aaataccgaa taccctacaa tatttaaaca
     3121 ttttccgttt acccccatac aacactatac cattatagga ttgcagaaca ataatccatt
     3181 acttacacac atacgatttg gtaaaggttc tttatttata tgtagttctc cacttacccc
     3241 acagagcaat acttttattt cacatccttt
//
LOCUS       DAOTOW010000190         3264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23163_length_3264_cov_4.067311, whole genome shotgun sequence.
ACCESSION   DAOTOW010000190 DAOTOW010000000
VERSION     DAOTOW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23163_length_3264_cov_4.067311"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..406)
                     /locus_tag="P5243_04390"
     CDS             complement(11..406)
                     /locus_tag="P5243_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18720.1"
                     /translation="MSSLHGKNEREENIVIAKYFAEKYNEKIDLLKNHHNKSSADAFN
                     HSKNEFQEYKTNKKPTKSAIDNELRKGANQSKHIIIHINSEISDDNLLRGIKGRVTQK
                     MNIEKITIIRNNQDKTYLREEIMQKGFKL"
     gene            complement(476..1285)
                     /locus_tag="P5243_04395"
     CDS             complement(476..1285)
                     /locus_tag="P5243_04395"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HRS18721.1"
                     /translation="MYGEHDLHLAKKTAEQRVFIDAAEYIYKNKGFTPEDLKKAPVRK
                     LIENINSVLTQAISEGLLDNSIKHEVGEDIKESLMNNVWLFSGMKTYYQCIEASAMLV
                     SADGKIKPFNEFKNDVIAIHEKYNERYLQSEYLFATHSSLMAAKWNDFEQDGDRYNLQ
                     YRTAGDELVRESHQILHNTTLPINDTFWDSYMPPLGWRCRCTVVQVRKSKYEISNSSE
                     AVIKGEQATTQLDANGVNKAAMFRFNPGKEKIVFPNKHPYMPRDSSPQDVK"
     gene            complement(1327..2526)
                     /locus_tag="P5243_04400"
     CDS             complement(1327..2526)
                     /locus_tag="P5243_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HRS18722.1"
                     /translation="MHSQELLKRLESSDKQRIKSMLIELVAKTQYLTKKDINTWRQAW
                     QMAINIENPQRSNLYDVYTDVDIDLHLSGCVGQRRDFVLKKSFKLVDFNTQKEDKNIT
                     ELFEEEWFKNFCEEILNSRYWGHSLVQLGDIVSVIGKKKFADITLVPRKHVIPEYGVI
                     VKEVGDEPKKGIDYRTGSIADWVIEAGGKKDLGLFLKCAPQTLPKKNMLGFWDAFGEM
                     FGMPIRIGKTTSRDSKEITKVERMLSDMGAAAWGLFPEGTEIEIKETQRGDAFEVYDK
                     RVERANSEISKGILNQTMTIDNGASKSQGEVHLEIFQNVVERDADFVKDIVNNKLIPI
                     MSKHGFGVEKYKFQWDESIDYTPEQQIQIDTLMVQNFDIDPQYFAKKYNIPIIGKKEA
                     SDTNFFE"
     gene            complement(2529..2954)
                     /locus_tag="P5243_04405"
     CDS             complement(2529..2954)
                     /locus_tag="P5243_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HRS18723.1"
                     /translation="MAFLEITDYKAVADDKTLSVIHQQSTTNLQRAEKYAAEEVSSYL
                     RSKYNMTQAFALTGDNRNQQLVMIMCDIALYHLISWLPLKMGFEIREIRYKRAIEWLE
                     SVQAGKASPELPPLIDSETGEDIGNPMKWGSMNKNKYDW"
     gene            complement(2956..3216)
                     /locus_tag="P5243_04410"
     CDS             complement(2956..3216)
                     /locus_tag="P5243_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18724.1"
                     /translation="MINYIIMLIELIKSIRKRKYSKLVTEAIEKANYFRNKTGYKCLV
                     LVIKGKPTVVTKKELKDRIKKNEFKKGTTIQDLEKIALYKTT"
BASE COUNT          847 a          603 c          511 g         1303 t
ORIGIN      
        1 gccttttttg ctatagttta aacccctttt gcattatttc ctctcttaaa tatgttttat
       61 cttgattatt acgaattata gttatttttt ctatattcat tttttgagtg actcgacctt
      121 taatacctct caataaatta tcatctgata tttcagaatt tatatgtata attatatgtt
      181 ttgattggtt tgcccctttt cttaattcat tgtcaattgc ggattttgta ggttttttat
      241 ttgttttata ttcttgaaat tcattttttg aatgattgaa tgcatccgca gatgatttat
      301 tgtgatgatt ttttaataga tcaatttttt cattatattt ttcggcaaaa tattttgcta
      361 ttacgatatt ttcttctcgt tcatttttgc catgtaaaga actcactctt actgttccat
      421 tagatgtttc aattgtttcc catttttcat tagtttcttc aattattttt gcagcttatt
      481 ttacatcttg cggactgcta tcgcgaggca tatatgggtg tttgttcggg aatactattt
      541 tttctttacc cggattgaat cgaaacatag ctgctttgtt tactccatta gcgtctaatt
      601 gtgtggttgc ctgttcgcct ttgattaccg cttcagaaga atttgatatt tcgtattttg
      661 atttacgcac ctgaactaca gtgcatctgc atcgccaacc aagaggaggc atatacgaat
      721 cccaaaatgt gtcgttaata ggtaatgttg tgttgtgtag tatttggtgc gattcgcgaa
      781 cgagttcgtc tcctgcagtt ctatattgaa gattataccg gtctccatct tgttcgaagt
      841 cgttccattt tgctgccata agcgacgaat gtgttgcaaa taaatattcg ctctgcaaat
      901 atcgctcgtt gtatttttcg tgaatagcaa tcacgtcatt tttaaactca ttgaatggtt
      961 taatttttcc atctgcagat acaagcatag ctgaagcttc tatgcattga taatatgttt
     1021 tcattcctga aaataaccac acattgttca tgagtgattc tttaatatct tcgccaactt
     1081 cgtgttttat agaattatct agcaatcctt cggaaatagc ttgagtaagc acactattaa
     1141 tattttctat taatttgcgt acaggagcct tttttaagtc ttcgggtgta aatcctttgt
     1201 ttttatatat atactcagca gcatcgataa aaacacgctg ttctgctgtt ttttttgcaa
     1261 gatgtaaatc atgttcgcca tacaacgata ctaatgcatt gtttagcccc tcatggtatg
     1321 aggggcttat tcgaaaaaat tagtgtcaga tgcttctttt tttcctataa ttggtatatt
     1381 gtattttttg gcaaaatatt gaggatctat atcgaaattt tgaaccatta gagtatctat
     1441 ttgtatttgc tgttcgggtg tgtaatctat agattcgtcc cattgaaact tatatttttc
     1501 tacaccaaat ccatgcttac tcattatagg aataagctta ttgtttacaa tatcttttac
     1561 aaaatcagcg tcgcgttcta caacattctg aaatatctct aaatgaactt cgccttggct
     1621 tttgcttgca ccgttgtcta ttgtcatagt ttgattgagt atgcctttac ttatttcaga
     1681 atttgcacgt tctacccgtt tgtcatatac ttcaaacgcg tctccacgtt gcgtttcttt
     1741 gatttcaatt tcggtacctt ctggaaaaag tccccacgct gcagcaccca tatcggaaag
     1801 catacgttct actttagtta tttccttaga gtctctgctt gttgttttcc ctatgcgtat
     1861 aggcattcca aacatttcgc caaatgcatc ccaaaaccca agcatgttct tttttggtag
     1921 tgtttgaggt gcacatttaa gaaataaccc taaatctttt tttcctcctg cttcaataac
     1981 ccaatcggca atagaacctg tacgatagtc aattcctttt tttggttcgt ctcccacttc
     2041 tttaactatc acaccatatt ccggtataac atgttttcgc ggtaccagtg ttatatcagc
     2101 aaattttttc ttaccaataa cgcttacgat gtcccccaat tgaactagtg aatgccccca
     2161 atagcgagag ttaagtattt cttcgcaaaa atttttaaac cattcttctt cgaacagttc
     2221 ggtaatgttt ttatcttctt tttgtgtatt aaaatcaacg agtttgaatg attttttcaa
     2281 cacaaagtct cggcgttgac caacgcatcc acttagatgg agatctatat ctacgtctgt
     2341 atatacatca tatagatttg acctctgtgg attttctata ttaattgcca tttgccatgc
     2401 ttgtcgccat gtatttatat cttttttggt aagatattgt gtttttgcaa caagttctat
     2461 aagcatcgat tttattcgtt gtttatcgct gctttctaac cgtttaagaa gttcttgtga
     2521 atgcataatt accagtcgta tttgtttttg ttcatactcc cccacttcat gggattacct
     2581 atatcttcgc ccgtttcaga atcaattagt ggtggcaatt cgggtgaagc tttaccagcc
     2641 tgtacgctct cgagccattc aattgcacgc ttgtatcgta tctcgcgaat ttcaaagccc
     2701 attttcagtg gtaaccacga gataagatga tataaagcaa tatcgcacat tatcattaca
     2761 agttgctgat ttctattatc accggtaaga gcaaatgcct gagtcatatt atacttactt
     2821 cttaaataag aactaacttc ttcagcagca tatttttcgg cacgctgcaa gtttgttgtg
     2881 ctttgttggt gaataacaga cagtgtttta tcgtctgcta cagctttgta gtctgtaatt
     2941 tcgagaaatg ccattttatg tagttttata tagtgctatt ttttctaaat cttgaatcgt
     3001 agttcctttt ttaaactcat tttttttgat gcggtctttg agttcttttt tagttactac
     3061 tgttggttta cctttaataa ctaaaacaag acatttgtat ccggttttat ttctaaaata
     3121 gttagctttt tcaatagctt cagttaccag ttttgaatac ttgcgtttac gtattgattt
     3181 tattaattct atgagcataa ttatataatt aatcattatt gtccgataaa actacaaaat
     3241 taatttaata aaacaaaatt tatc
//
LOCUS       DAOTOW010000191         3252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23272_length_3252_cov_3.651236, whole genome shotgun sequence.
ACCESSION   DAOTOW010000191 DAOTOW010000000
VERSION     DAOTOW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23272_length_3252_cov_3.651236"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1583
                     /locus_tag="P5243_04415"
     CDS             351..1583
                     /locus_tag="P5243_04415"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRS18725.1"
                     /translation="MAYINEHYEKLQAGYLFPEIGRRVNAYAAKNPDKKIIRMGIGDV
                     TKPLVPAVIDAFHAGVAEMAQSDTFKGYGPEQGYAFLREAIAKHEFQDKGIAVSADDI
                     FISDGSKCDTGNILEIFGMKNIIAVQDPVYPVYVDTSVMAGRTGTMQANGYYDNIVYM
                     PCNKDNKFIPELPKQKVDIIFLCYPNNPTGTTATKEELKKWVDYARVNNAIILFDAAY
                     EAYITESNVPHSIYEIEGAETCAIEFRSFSKKAGFTGVRCAYIIVPSTVMAKNSQGED
                     VSLKTLWSRRHSTKFNGASYPVQKAAAAIYTPEGKKQVDEVIAYYLENARIMKQSLEQ
                     QGFTVYGGVNAPYVWVQTKNGMKSWDFFDQLLEQIQVVGTPGSGFGPSGEGYFRFSAF
                     AARENVIEAMARLQNIKG"
     gene            complement(1563..>3252)
                     /locus_tag="P5243_04420"
     CDS             complement(1563..>3252)
                     /locus_tag="P5243_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18726.1"
                     /translation="MGFKKLATSVGDARFRIKNPSGTVVYGFTNIPTSGAGYISTFSQ
                     ADIGPNTLTGGSGGYTPLTYTTTTTGDFYIEFEKTTDPTARYLFEFFDITVSSGTTPK
                     NGRVWSYAWDLNTASFTNSAYAKFYVYSADRYVTAINLNGMQPYSFVIACNNSGVQNT
                     GDLFVDRQSVPNTNTVRPQYKIFLNTPDPAVYPVAEIPTMVENLRINGTPTANQPVEF
                     YINMTKAGTLEIFLDIDGVPGYQSGGRDVVLVEYINAGGDVLYWDGKDGLGDQVTSTI
                     SVIVSSKFATGVTHLPVYDAEKNPNGFLVTRVSPDGPTDLVLYWDDSQIPYGTIPSGQ
                     VITMLSGNTTANGHKWTTDSNNSDGFGNVHTINTWWNGYEIDNLASFDFILLPIELME
                     WKAELKGTYVQLNWSTASENNNHLFNVQRSADGITWETISTIVGAGNSTQTMYYTEID
                     ENPLQTIAYYRLQQVDFDGVYSYSPIIAIGASTQPKQKVQVYSVQGNRFISYDNYTSL
                     IEQIDIINSQGKSVLSYVTIQKNSLSNIKIDVTRLPKGMYIFKSPQGASAFYIL"
BASE COUNT         1051 a          566 c          570 g         1065 t
ORIGIN      
        1 gaaaaaatta ttgatttgat ttgatttgat ttgatttgat tttcatagtt taaaaatttt
       61 aaaagtttat tatttttttt aaggggatta ttgtatagtt acatatctta tatgcattac
      121 tatataaata caaagattta tgaggcgacc tcctttcttt ttctattgat taattatgct
      181 ctttacaaga ttgattatag gcaaaaatac cacattgttt ttttatgaac aaatatttgt
      241 attgaaattc tcatgatttt ttacgcacgt atattttgtt tcaaatattg tttcagaaat
      301 ttgcgaatag aaggctttca agtatatttg caaaaaaaat tataacatat atggcttata
      361 taaacgaaca ttacgagaaa ttacaagcag ggtatttatt ccccgaaata ggacgacgtg
      421 taaatgcata tgcggcaaaa aatccggata aaaaaattat tcgtatgggt attggtgatg
      481 ttaccaaacc acttgttccg gctgtaattg atgcttttca tgccggtgta gcagaaatgg
      541 ctcaaagtga tacgtttaaa ggatatggcc ccgaacaagg ctatgcattt ttgcgtgagg
      601 ctatagcaaa acatgaattt caagacaaag gaatagcggt gtctgccgat gatatattta
      661 tctctgacgg ttcaaaatgt gataccggaa atattctcga aatttttggc atgaaaaaca
      721 tcattgcagt gcaagaccct gtgtacccag tgtatgttga cacgtctgta atggctggta
      781 gaaccggcac tatgcaagct aatggttatt atgacaatat tgtatatatg ccgtgcaaca
      841 aagacaataa gtttataccc gaactaccaa aacaaaaagt agatattata tttttatgtt
      901 atcccaataa tccaacagga actactgcaa caaaagaaga actaaaaaaa tgggttgatt
      961 atgcgcgtgt aaataatgct attattctat ttgatgctgc atacgaagca tatattaccg
     1021 aaagtaatgt gcctcattca atatatgaaa tagaaggagc cgaaacatgt gctattgaat
     1081 tcagaagttt ttcgaaaaaa gcagggttta ccggtgttcg ttgtgcatat atcatagtgc
     1141 caagcacagt aatggcaaaa aattctcaag gagaagatgt aagtcttaaa actctttgga
     1201 gtcgtcgtca ttctactaaa ttcaatggcg cttcgtatcc ggtgcaaaaa gctgctgcag
     1261 ctatttatac tcccgaaggt aaaaaacaag tagatgaagt aattgcttac tatttggaaa
     1321 atgctcgcat tatgaaacaa agtttggagc aacaaggatt taccgtgtat ggtggagtaa
     1381 atgctccgta tgtatgggtt caaaccaaaa atggtatgaa atcttgggac ttttttgatc
     1441 aacttttaga acaaattcaa gttgtaggaa ctccgggctc aggttttggt ccaagtggcg
     1501 aaggatattt ccgtttttcg gcatttgctg ctcgcgaaaa cgtgatagaa gctatggcac
     1561 gcttacagaa tataaaaggc tgatgctccc tgcggacttt tgaaaatata cattccttta
     1621 ggaagtcgag taacatctat tttaatattt gataaagaat tcttttgaat cgtaacatac
     1681 gaaagaactg atttgccttg cgaatttata atatctattt gttcaattag agacgtataa
     1741 ttgtcatagc ttataaatcg gttaccttga accgagtata cttgtacttt ttgtttcggt
     1801 tgcgttgaag ctcctattgc tataatagga gaatatgaat aaacaccgtc aaaatcaact
     1861 tgttgtaatc ggtagtatgc aatagtttgt aatggattct catctatttc ggtatagtac
     1921 atagtttgtg ttgaattacc tgctcctaca atagttgaaa ttgtttccca tgtgattcca
     1981 tctgccgaac gttgaacatt aaacaaatga ttattgtttt ctgatgctgt tgaccaatta
     2041 agttgaacat atgtgccttt taattctgct ttccattcca taagctcaat tggcaatagt
     2101 ataaaatcaa aagatgccaa attatcaatt tcatatccgt tccaccaagt atttatagta
     2161 tgtacgttac caaatccatc gctattattg ctatcggtag tccatttatg accatttgca
     2221 gttgtattac ccgaaagcat tgtaataact tgccccgacg gaattgtacc atatggaatt
     2281 tgtgaatcgt cccaatacaa aaccaaatct gtaggaccat cgggactgac acgagtaact
     2341 aaaaaaccat taggattttt ctcagcatca tacacaggta aatgggttac ccctgttgca
     2401 aattttgacg aaactattac agaaatagtt gaagtaactt ggtcgcccaa accatcttta
     2461 ccatcccaat ataatacatc gccacctgca tttatatatt ctaccaatac cacatctctt
     2521 ccacccgatt gatagcccgg aaccccatct atatcaagaa atatttcgag agttcctgct
     2581 tttgtcatat ttatataaaa ttctaccggc tgatttgcag taggtgttcc attaatgcgt
     2641 aaattttcaa ccattgttgg aatttctgct accggataca ctgcagggtc gggagtattt
     2701 aaaaatattt tgtattgcgg acgaactgta ttggtatttg gtaccgattg cctatctaca
     2761 aataaatcac cggtattttg cacaccacta ttattacatg ctataacaaa actatatggt
     2821 tgcataccat ttaaattaat tgcagttaca tatctatcgg cactatatac ataaaatttt
     2881 gcataggcac tattggtaaa acttgctgta ttcaaatccc atgcatacga ccacactctt
     2941 ccatttttag gagtagttcc ggaacttaca gtaatatcaa aaaattcaaa caaatatcga
     3001 gctgtaggat ccgtagtttt ttcaaattct atataaaaat cacctgttgt agttgtagta
     3061 tacgttaaag gagtatatcc tccactccca ccggtaagag tattcggtcc tatatctgct
     3121 tgactaaatg tacttatata tcctgcaccc gaagttggaa tatttgtaaa accatacact
     3181 actgtgcccg aaggattttt tatacgaaac cgtgcatcac caacagatgt agctaatttt
     3241 ttaaatccca ta
//
LOCUS       DAOTOW010000192         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23457_length_3230_cov_3.022677, whole genome shotgun sequence.
ACCESSION   DAOTOW010000192 DAOTOW010000000
VERSION     DAOTOW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23457_length_3230_cov_3.022677"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="P5243_04425"
     CDS             <1..942
                     /locus_tag="P5243_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337157.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRS18727.1"
                     /translation="AGDKFHMILSKDNPQFQNLPGIAVLIGGMWIANLYYWGFNQYII
                     QRALAAKSLHEAQKGLSFAAFLKLIIPAIVVIPGIIAYVMYIDNSNNAGAIFSLADGA
                     NNDHAYPWLIKTFIPIGLKGLVVAALTAAIVSSLASMLNSTATIFTMDIYKPYFAKNK
                     QDSHLVNVGRITALLSLFIAGFLAPAMSSFPQMFQYIQEYTGVVSPGILAVFIMGLFW
                     KKTNAQGAIAGVLASIPVALALKFLPIQMPFLDQMMYTCIITIVIILGVSLSTGKGDD
                     NHKSIPLIGNTFSTTKGFNISAYAICIVLVVLYSVFW"
     gene            complement(959..2245)
                     /gene="rimO"
                     /locus_tag="P5243_04430"
     CDS             complement(959..2245)
                     /gene="rimO"
                     /locus_tag="P5243_04430"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRS18728.1"
                     /translation="MAKTVNIITLGCSKNTVDSERLARQLGAVGYEISFDSNIFSDIV
                     IINTCGFIHDAKEESIEMILQAIHAKEAGEIESVYVIGCLSERYTNDLRTEIPEVDSY
                     FGARGFIDIIQELHAQANKNILHERIVSTPKHFSYLKIAEGCNRSCAFCAIPAIRGAH
                     ISEPIEQLFNEALYLQSVGVKELSIISQDLSYYGYDLYKKNALYDLLEKLISIQGIEW
                     FRLHYLYPNNFPLNILQLMKHSSKICNYIDIPFQHISDTVLSYMKRGFTEKETWKLIE
                     NIRKIIPDIAIRTTLIVGHPGETDMEFNKLHDFVRQVGFDRLGVFTYSHEEGTYAGDN
                     YKDEIPEKIKQERLDILMNTQQDISLKKNMSMVGTIQQVIIDRKEQNSYIGRTQFDAY
                     EVDNEVIITTEKLLEIGNFYNVTITDADVYDVFATV"
     gene            complement(2314..>2629)
                     /locus_tag="P5243_04435"
     CDS             complement(2314..>2629)
                     /locus_tag="P5243_04435"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRS18729.1"
                     /translation="FPIVLHGASSVPQEYVATINSNGGALKDTIGIPEDQLRRAAASA
                     VCKINIDSDGRLAMTAAVRKFFNENPDKFDPRQYLGPAREELKKLYTHKIQNVLGSAG
                     KA"
     assembly_gap    2630..2639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2640..>3230)
                     /locus_tag="P5243_04440"
     CDS             complement(<2640..>3230)
                     /locus_tag="P5243_04440"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRS18730.1"
                     /translation="AFNFNNLEQLQAIVSACAEQKSPVILQVSSGARKYANQTLLRFL
                     AQGAVEYAKELGWEKPQIALHLDHGDTFELCKSCIDMGFSSVMIDGSHHSFEENIALT
                     KKVVEYAHAHGVTVEGELGVLAGVEDDVVAEHSTYTQPAEVVEFVQRTGVDSLAISIG
                     TSHGANKFTPKQCTRNAEGLLVPPPLRFDILEEIEKQ"
BASE COUNT         1066 a          541 c          481 g         1132 t
ORIGIN      
        1 gctggtgata aatttcatat gatattatca aaagataatc ctcaatttca aaatttgccc
       61 ggaatagctg tgcttattgg cggtatgtgg atagctaatt tatattattg gggatttaat
      121 caatatataa ttcaacgagc acttgccgca aaatctttgc atgaggcaca aaaaggatta
      181 agtttcgcag cttttcttaa acttataata cctgctatag tagtaattcc cggtataata
      241 gcatatgtaa tgtatataga taattccaat aatgctggtg caatattttc tttagcagat
      301 ggagccaata atgaccatgc atatccatgg cttattaaaa cttttattcc tattggtctc
      361 aaaggattgg tagtggcagc attaacagcc gcaattgttt catcacttgc atcaatgctt
      421 aattctacgg ctactatatt tacaatggat atttacaaac cttattttgc aaaaaacaaa
      481 caagattctc atttagtgaa tgtaggaaga attacagctt tactatcatt atttatagca
      541 ggatttttag ctcctgctat gagttctttt cctcaaatgt ttcaatatat acaagaatat
      601 actggagttg ttagtccggg tatattagca gtatttataa tgggattatt ttggaaaaaa
      661 acaaatgctc aaggagcaat tgccggcgta cttgcttcaa ttccggttgc tttagctctt
      721 aaatttttac ctatacaaat gccattttta gatcaaatga tgtatacgtg tattattaca
      781 attgtaatta tcttaggagt aagtttatca acaggaaaag gagatgataa tcataaatca
      841 ataccattga taggtaatac attttctaca acaaaaggat ttaatatatc ggcgtatgca
      901 atttgtattg ttttagttgt gttgtatagt gttttttggt aataataaga tattcaagtt
      961 atacagtagc aaaaacatca tatacatcgg catcggttat tgtaacatta taaaagttac
     1021 caatttctaa tagtttttct gttgtaatta ttacttcatt gtcaacttca tatgcatcaa
     1081 attgagttct accaatatat gagttttgtt cttttctatc aattatcact tgttgaatgg
     1141 tacctaccat actcatattt tttttcaaag atatatcttg ttgagtattc ataagtatat
     1201 ctaatcgttc ttgttttatc ttttccggaa tttcatcttt ataattgtct cctgcatacg
     1261 taccctcttc atgagaataa gtaaacacgc ctaacctatc aaatccaact tgccgtacaa
     1321 aatcatgtaa tttattgaat tccatatctg tttcacccgg atgaccaaca ataagtgtag
     1381 tgcgaatagc aatatcagga attatttttc ttatattctc aattaatttc cacgtttctt
     1441 tttctgtaaa tccccgtttc atatatgata gtacggtatc agaaatatgt tgaaacggaa
     1501 tgtcaatata attacaaatt tttgaggaat gtttcattaa ttgcaatata tttaaaggaa
     1561 aattatttgg atacagataa tgcaaacgaa accattcaat accttgaata gaaattaatt
     1621 tttctaacaa atcatacaat gcattttttt tatataaatc atatccgtaa tatgacaaat
     1681 cttgtgaaat aatagataat tcttttacgc caactgattg taaatataaa gcttcgttaa
     1741 ataattgttc aattggttca gatatatgag cacctcttat agcaggaata gcacaaaaag
     1801 cacaactcct attacatcct tcagcaattt taagatatga aaaatgctta ggagtagaaa
     1861 caatacgctc gtgcaaaata tttttatttg cttgtgcatg tagttcttga ataatatcta
     1921 taaaaccacg ggctccaaaa tatgaatcaa cttcgggaat ctctgttcgt aaatcatttg
     1981 tataacgttc agataggcaa ccaataacat atacactttc tatctcacct gcttctttag
     2041 cgtgaattgc ctgcaaaatc atttcaattg attcctcttt agcatcgtgt ataaaaccac
     2101 aggtattaat aataacaata tcagaaaaga tattagaatc aaaggaaatt tcatatccaa
     2161 cagcgccaag ttgtcttgca agtcgttcac tgtcaactgt atttttagaa cacccaagtg
     2221 ttataatatt aactgttttt gccatattac aaataaaata aaaaaggtcg ccatgaatta
     2281 cagcgacctt ttacatatca tattcttatt agattatgct ttacctgcag aacctaatac
     2341 attttgaatt ttatgagtat ataatttttt taattcctca cgagcaggac ctaaatattg
     2401 acgtgggtca aatttatcag ggttttcgtt gaaaaattta cgaacagcag cagtcatagc
     2461 taaacgacca tcagaatcaa tattgatttt gcacacagca gatgcagctg ctctacgaag
     2521 ttgatcttcc ggtataccaa tagtatcttt taatgcacct ccgtttgaat taattgtagc
     2581 aacatattct tgaggaacag aagatgcacc atgtaatact ataggaaatn nnnnnnnnnt
     2641 tgtttttcaa tttcttctaa tatatcaaaa cgtaacggag gaggaactaa taacccttct
     2701 gcattacgag tacattgttt tggtgtaaat ttgttagcac catgtgatgt gcctattgat
     2761 atagctaaag aatcaacacc ggtacgttga acaaattcta ctacttcagc aggttgtgta
     2821 tatgtagagt gttcagcaac aacatcatct tctactccgg ctaaaacgcc aagttcacct
     2881 tctacagtta caccatgagc atgtgcatat tctactactt ttttagttaa tgctatattt
     2941 tcttcgaatg aatgatgaga accatctatc ataactgaag aaaatcccat atctatacag
     3001 cttttacata attcaaaagt atctccgtgg tctaagtgta aagcaatttg aggtttttcc
     3061 catcctaatt cttttgcata ctcaactgct ccttgagcta agaaacgtaa taatgtttga
     3121 tttgcatatt tacgtgctcc acttgaaact tgtaaaataa caggagattt ttgttcagca
     3181 caagctgata caattgcttg taattgttcc aaattattga aattgaacgc
//
LOCUS       DAOTOW010000193         3225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23506_length_3225_cov_3.750158, whole genome shotgun sequence.
ACCESSION   DAOTOW010000193 DAOTOW010000000
VERSION     DAOTOW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23506_length_3225_cov_3.750158"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="P5243_04445"
     CDS             complement(<1..850)
                     /locus_tag="P5243_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS18731.1"
                     /translation="MAQQDMPLLQGTVEDVSKQPLVGASVFWADGSNAVLTDTKGIFA
                     IPYNSTQSRLVASYVGYTSDTLEIPIGKKGTVQFILQQGVELQTVEVKHKITSTFSSS
                     LEPMKIQVMTEHELNKAACCNLSESFETNPSVDVSFTDAVTGTKQIQMLGLAGPYIQI
                     SRENMPYIRGLSAVHGLTFVPGPWVQAIHLNKGAGSVVNGFESITGQIDVQYRNPFTM
                     DKFFLNLYASPSGRYEANVATKYSVAEHMSTALLLHGKTSQARHDSNNDGFMDMPKGS
                     LLTLANR"
     gene            complement(904..1428)
                     /locus_tag="P5243_04450"
     CDS             complement(904..1428)
                     /locus_tag="P5243_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRS18732.1"
                     /translation="MKTIITIVFVSIGLLTFTSTQAQEKACCAKSGVNKEQCCASTSK
                     ECPKKDTCTKRESCSKKESCPKQSSHTQTSTKQGIVTETFTVMGSCSMCKTRIENAAL
                     VKGVKSAEWNKDSHQITVIYDCSKVTSQAIHNSIAQAGHKTSLVEPNMEAYNKLPKCC
                     AYLQGGGSSCGHSH"
     gene            complement(1526..1984)
                     /locus_tag="P5243_04455"
     CDS             complement(1526..1984)
                     /locus_tag="P5243_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18733.1"
                     /translation="MKKIVTIAIFGIYLLLQVGIITHVHMCHNSIADIQLFDKSNISC
                     DIHENYECCKTENKQKSCCNAHATCSIKNSQTHESCCTNTTIILQYLNNTQYVSQSPS
                     IQLYPIHIAKQMLITYENTSERVQSNLFIEYSSPPPEDLVSLYCSYIFYG"
     gene            2177..3106
                     /locus_tag="P5243_04460"
     CDS             2177..3106
                     /locus_tag="P5243_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18734.1"
                     /translation="MDKTVRTLDYEKILRILEDTEEKYVFEKVNLVPKEDDLETKLRN
                     YVNYSKMNRKAVLGIDIYKYSSYEPFEQILVPVMFKLLFRETIEMCFKNHAFFFQKYT
                     KEEIEENFISTGDGGFVIFDIPLHALLFACNFAIALRTYNSYHFYPKLRKIIGGASLR
                     YAITYDNLYYFDKSHYGRAIINNARILNKDTLNRCIIDQNVYDWFTLNIDGMENLQLL
                     TIDDIASIYDIQGSYDFQILEEHPDAIFGTTSTRESGIINSDVLKIGEIQSKESTLSV
                     YNIHVQVASKWQRTDSQRMITVSLGNLNTSHLL"
     gene            3111..>3225
                     /locus_tag="P5243_04465"
     CDS             3111..>3225
                     /locus_tag="P5243_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRS18735.1"
                     /translation="METVLSGIRPTGNLHLGNYFGAVQNFIKMQESYNCFFF"
BASE COUNT         1023 a          482 c          574 g         1146 t
ORIGIN      
        1 agcggttggc gagtgtgagc aggctgcctt tgggcatatc cataaatcca tcattgttgg
       61 aatcgtgacg agcttgcgat gttttgccat gtaaaagcaa agcggtagac atatgctcgg
      121 caacagaata tttagttgcc acatttgctt catatctacc cgatggactt gcatatagat
      181 tgagaaaaaa tttgtccata gtaaagggat tccgatattg aacatcaatt tgtcctgtta
      241 tactttcgaa tccgttaaca acagatcctg cacctttgtt gaggtgaatg gcttgcaccc
      301 atggcccggg aacaaatgta agtccgtgta ctgcagaaag accgcgtata tacggcatat
      361 tttctctgct tatttgaata tacggacctg ccagtcctaa catttgtatt tgtttagtac
      421 cggttacagc atcggtaaag gatacatcta ccgatggatt tgtttcgaaa ctttcgctca
      481 aattacagca cgccgctttg ttgagttcgt gttcagtcat tacctgaatt ttcataggtt
      541 ccaatgaact tgaaaatgtg ctggtaattt tatgttttac ttcaactgtt tgtaattcta
      601 ctccttgttg taaaataaat tgaacagttc cttttttgcc aataggaatt tctaatgtat
      661 cagatgtata cccaacatac gaggctacta aacgtgattg tgttgagttg tatggaatgg
      721 caaatatacc tttagtatca gtaagtacag cattactccc atcagcccaa aatacacttg
      781 cacctacaag tggttgtttg ctaacatctt ctacagtgcc ctgtaacaaa ggcatatctt
      841 gttgtgccat aagatatgtg ttgctcaccg cagtgagcaa cagcactatt aaataagacc
      901 tcattaatga gaatgtccgc aactactacc accaccttgt aagtaggcac aacatttagg
      961 aagtttgttg tatgcttcca tatttggttc tacaaggctt gttttatgtc cggcttgagc
     1021 tatagaattg tgtatagctt gtgacgtaac tttagaacag tcataaatta cagtaatttg
     1081 gtgcgagtct ttgttccatt ctgccgattt tacaccttta acaagggcag cattttctat
     1141 acgtgtttta cacattgaac aagatcccat tacagtaaac gtttcggtaa ctataccttg
     1201 tttagttgat gtttgtgtat gagaactctg ttttgggcat gactcttttt ttgagcatga
     1261 ttctcgttta gtgcacgtat cttttttggg acattctttt gatgtacttg cgcaacattg
     1321 ttctttgttt actcctgatt ttgcacaaca tgctttttct tgtgcttgtg ttgaggtaaa
     1381 tgtaagtaat cctatgctta caaatactat tgttataatt gttttcattg ttctactttt
     1441 taattggtaa ataattgtta gttttgagtt atataataaa acaaatacat tgtttgtaat
     1501 atataattat ctacacacaa acaaattacc cgtaaaaaat atatgaacaa tacaaggaaa
     1561 ctaaatcttc gggaggagga gaggaatatt caataaataa atttgattgt actctttcag
     1621 aagtattttc ataagttata agcatttgtt tagcaatatg tattggatat aattgaatag
     1681 aaggcgattg agaaacatat tgggtattgt ttaaatattg caaaataata gttgtatttg
     1741 tacaacaact ttcatgagtt tgtgagtttt ttatactaca tgtggcgtga gcattacaac
     1801 atgatttttg tttgttttca gttttacaac attcataatt ttcatgtata tcacaagaaa
     1861 tatttgattt gtcaaataat tgtatatctg ctatagaatt atgacacata tgcacatgtg
     1921 ttatgatacc tacttgtaat aataggtata taccaaatat tgctatggtt acaatttttt
     1981 tcattgtata tacataaagt atgtgttaca aatatacatg taaaaacaac aatttagact
     2041 tacatgttgt gtaaagttta catgtaattt ttgctgagta taattaatta atagtatgat
     2101 ttttttatag aaatcattat gtgaatatat gctacttgag tgtaaaagtg tacatttgta
     2161 aaaaaaatgc aatggtatgg ataaaactgt tcgcacactc gattacgaga aaatcctgag
     2221 aatcttagaa gatactgagg aaaaatatgt ttttgaaaaa gtaaacttag ttcctaaaga
     2281 agacgatttg gagaccaaat tacgtaatta tgttaattac agcaaaatga atcgtaaagc
     2341 ggtgctgggt attgacattt acaaatacag ttcatatgag ccgtttgaac aaattctggt
     2401 accggtaatg tttaaattac tatttcgcga aaccatagaa atgtgtttta aaaatcatgc
     2461 tttctttttt caaaaatata ctaaagaaga aatagaagaa aactttattt caaccggtga
     2521 cggaggtttt gtaatttttg atataccgct tcatgcatta ctgtttgctt gtaattttgc
     2581 tatagctctt cgaacatata attcatatca tttttatcca aaacttcgta aaattattgg
     2641 aggagcaagt ttgcggtatg ctataaccta tgataatttg tattattttg ataaaagtca
     2701 ttatggtaga gcaattataa ataatgctcg tatcttaaat aaagatacac tcaatcgttg
     2761 tattattgac caaaatgtgt atgattggtt tacgttgaat attgatggta tggaaaattt
     2821 gcaattgctt actattgatg atattgcaag tatttacgat attcagggca gttatgattt
     2881 tcaaatttta gaagaacatc ccgatgcaat ttttggtact acatcaactc gcgaaagtgg
     2941 aattataaat tccgatgttt taaagattgg tgaaattcaa tcaaaggaat ctacgctgag
     3001 tgtttacaat attcatgtgc aagttgcaag taagtggcaa cgaacagatt cgcaacgtat
     3061 gattacagta agtttaggca atttaaatac atctcattta ttatagaaat atggaaacag
     3121 tgttaagtgg aattcgtcct acaggaaatt tacatttagg aaattatttt ggtgcagtac
     3181 agaattttat aaaaatgcag gaatcataca actgcttttt cttta
//
LOCUS       DAOTOW010000194         3205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23704_length_3205_cov_2.825714, whole genome shotgun sequence.
ACCESSION   DAOTOW010000194 DAOTOW010000000
VERSION     DAOTOW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23704_length_3205_cov_2.825714"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="P5243_04470"
     CDS             <1..1005
                     /locus_tag="P5243_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS18736.1"
                     /translation="KLTISVSETDNVSANAEIAIECNPADIDLAYISAITALGFNRIS
                     LGIQDFDAAVLQAVHRNVPTISIEAFVETIKTANAYVNFDFIYGLPLQTPQQFKRTIE
                     QAIALQPDRLVTFSYAHVPSVKPHQKHLEQHAMPTAHNKLEMLTNTLQCMQQAGYTSI
                     GFDHFAKPADSLSVAKKNRTLHRNFQGYCTRETTGQVFAFGASGITQLADSYFQNVKD
                     IQTYTQNIASQFVAPHVGYCLSHDEIITAAVIEYILCNESLNWQAIAVNIGEAEHTVR
                     ALFPNYMELCQEFQRDGLLEFSQYGFDVTLPGKFFLRNIAAAFDSQFVNGSKIFSKTI
                     "
     gene            1009..2370
                     /gene="hemG"
                     /locus_tag="P5243_04475"
     CDS             1009..2370
                     /gene="hemG"
                     /locus_tag="P5243_04475"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRS18737.1"
                     /translation="MHKTHVAIIGGGLTGLSIAYFLSQRGISCTIFEQHDRCGGVICT
                     IHHEGFTFECGPNTGVLGNVEIEKLFDELQTSCTLETAQKSAKVRLIWKQGAWRALPS
                     GLWSAITTPLFTFFDKLRILGEPFRKAGTNPNETVYELVLRRMGKSFHDYAVAPFING
                     VYAGNTKTLVTRFALAKLYALEQTYGSFIRGAIAKAKYNKKHGIPTPSKQVFTCKGGL
                     QNLITALEKNSKATIFTQCQNVLVEPFQNEYNVSFTLNGQQENKTFSHVITTCNAPSL
                     PRVLPWLSTYHTQIFESVEYAPVVQVAVGFKKWKGMDIQAFGGLVPPCENRDILGVLF
                     PSSMFSNRAPQGGAMLSVFLGGMLRPDIIHKTDDEITAIVARELQHMLSIPQWNPDLV
                     RIFRYTHAIVQYTKHMEQVFASIDEIEHTHTNLILAGSVRNGIGMADRVLQAWNIAQR
                     LSI"
     gene            2459..>3205
                     /locus_tag="P5243_04480"
     CDS             2459..>3205
                     /locus_tag="P5243_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18738.1"
                     /translation="MKQLLICISFVIALISCNSTDNSRVSVIYPFNNCVFPREFPAPG
                     IRWTTDSVTKQWTVEIVHDGTVEISATIDTCMWIPEQSAWEQLKSKAVEKKIECIVYC
                     IDAKGKKQASSSIEFKISKDSVGAPIFFRSVILPFSKANQHKDSLRWKLGNIASAEEP
                     RLMLTNIPVCANCHSFSADGKKFAMDVDNSNNKGSYTITDISNGISDISLNTIITWDE
                     YKKEDGEQTFGLLAQISPDGKNVISMLKDRS"
BASE COUNT         1077 a          515 c          606 g         1007 t
ORIGIN      
        1 aaactaacta tttctgtctc tgaaactgat aatgtttctg caaatgctga aattgctatt
       61 gaatgtaatc ctgccgatat tgatttagca tatatttctg ctattactgc attgggattt
      121 aatcgtataa gtttaggtat tcaggatttt gatgccgctg tgttgcaagc ggtacatcgt
      181 aatgtgccaa ctatttcaat tgaagcgttt gtagaaacaa tcaaaactgc aaatgcatac
      241 gtaaattttg atttcattta tggactgcca ttacaaacac cacaacaatt taaacgaact
      301 atagaacagg caatagcttt acaacccgat agattggtaa ctttttcgta tgctcatgtg
      361 ccttcggtaa aaccacatca aaaacattta gaacaacatg caatgccaac ggctcacaat
      421 aaattagaaa tgcttaccaa tactttacag tgtatgcagc aagccggata tacatcgata
      481 ggatttgatc attttgcaaa gccggccgat agtttgagtg tagccaaaaa gaatagaaca
      541 ctgcatcgta attttcaagg ttattgtact cgtgaaacta ccggtcaagt atttgctttt
      601 ggtgcttcgg gtattacaca actagctgat tcatattttc agaatgtaaa agatattcaa
      661 acctatactc aaaatattgc atcgcaattt gttgctccgc atgtaggata ttgcttgtcg
      721 cacgacgaaa taattactgc tgcagtaatt gaatatattt tgtgtaatga gagtctcaat
      781 tggcaggcta ttgcagtaaa tattggagaa gctgaacata cagtacgtgc attgttccct
      841 aactatatgg aattgtgcca agaatttcaa cgcgatggtt tgttggaatt ttcacagtat
      901 ggatttgatg taacactacc aggtaaattt ttccttagaa atatagctgc tgcttttgat
      961 tctcaatttg taaacggaag taaaatattt tcaaaaacaa tttagaatat gcacaaaaca
     1021 catgttgcaa ttattggagg agggttaaca ggtttaagta ttgcatattt cttatctcaa
     1081 cgtggtattt cgtgtactat atttgaacaa catgatagat gtggaggggt aatatgtaca
     1141 attcatcacg aaggttttac atttgaatgt ggccccaata ccggagtttt aggtaatgtt
     1201 gaaattgaaa aattatttga tgaattacaa acaagttgta ccctcgaaac tgctcaaaaa
     1261 tcggcaaagg ttagattgat atggaaacaa ggtgcatggc gagcattacc ttcgggattg
     1321 tggtcggcaa ttacaactcc tttatttaca ttttttgata aattacgaat acttggagaa
     1381 ccatttcgta aggcaggaac caatccaaat gaaactgtgt atgaacttgt tcttcgcaga
     1441 atgggtaaaa gttttcatga ttatgcagta gctccattta taaatggtgt gtatgccgga
     1501 aataccaaga cgttagtcac tcgttttgca ttggcaaaat tatatgcatt agaacaaact
     1561 tatggtagtt tcatacgagg agcaatagcc aaggcaaaat ataataaaaa acatggtatt
     1621 cctacccctt caaaacaagt gtttacttgt aaaggtgggt tacaaaactt aattactgca
     1681 ttagaaaaaa attccaaagc aactatattt acgcaatgtc aaaatgtatt agttgaacca
     1741 ttccaaaatg aatataatgt tagttttaca ctcaatggtc aacaagaaaa taaaacattt
     1801 agtcatgtaa taactacctg caatgctcct tcattacctc gtgtgttgcc atggttatct
     1861 acgtatcata cacaaatatt tgaatcagta gaatatgctc cggtagtaca agttgccgtt
     1921 gggtttaaga aatggaaggg catggatata caagcatttg gaggtttggt gcctccttgc
     1981 gaaaatagag atatattggg agtgttattc ccttcatcta tgtttagtaa cagagcaccg
     2041 caagggggag caatgttatc tgtgtttttg ggaggaatgt tgcgacctga tataattcat
     2101 aaaacagatg atgaaattac agcaatagta gcacgcgagc tgcaacatat gctttctatt
     2161 ccccaatgga atcctgattt ggtaagaatt tttagatata cacatgctat agtacaatat
     2221 acaaaacaca tggaacaagt atttgctagt attgatgaaa tagagcatac acatacaaat
     2281 cttatacttg ccggaagtgt tcgcaatggt ataggtatgg cagatagggt actacaggca
     2341 tggaacattg cacaacggtt atcaatttaa ttttgctgtt gttgtaatta agcatagtaa
     2401 atcggtcata ctattacaat aaaaatatta tttttgcata gtttttaaac aatgtactat
     2461 gaaacaatta ttaatctgta tatcatttgt tatagcgttg atttcatgta attcaactga
     2521 taattcacga gtgtctgtta tatatccgtt taataattgt gtgttcccgc gtgaatttcc
     2581 cgctcccggt attcgttgga ctaccgattc tgttaccaaa caatggacgg tagaaatagt
     2641 acacgatggt accgttgaaa tttcggcaac tattgatacg tgtatgtgga ttcccgaaca
     2701 aagtgcttgg gaacaattga aatcaaaagc ggtagagaaa aaaattgaat gtattgtata
     2761 ttgtattgat gcaaaaggta aaaaacaagc ttcaagttca atagaattca aaatttcaaa
     2821 agattcggtt ggagcaccca tcttttttag atctgttatt ttaccgttta gtaaagcaaa
     2881 tcaacataaa gattcattgc gttggaaatt gggcaatatt gcatctgccg aagaaccacg
     2941 attgatgttg acaaatattc ctgtatgtgc caattgtcat agtttttctg ccgacggaaa
     3001 gaaattcgca atggatgtag acaactcaaa taacaaaggt tcatatacta ttaccgatat
     3061 ttcaaatggc ataagtgata tatcattgaa tacaataatt acttgggacg aatataaaaa
     3121 agaagatgga gaacaaacat ttggattact agcacaaata tcacccgatg gaaaaaatgt
     3181 gataagtatg ctcaaagacc gttct
//
LOCUS       DAOTOW010000195         3180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23922_length_3180_cov_4.644480, whole genome shotgun sequence.
ACCESSION   DAOTOW010000195 DAOTOW010000000
VERSION     DAOTOW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23922_length_3180_cov_4.644480"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="P5243_04485"
     CDS             <1..1679
                     /locus_tag="P5243_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRS18739.1"
                     /translation="FGLDGWWIDSTEPDVTNALTKESTDYEMKNKVGKNHLGSWDRYL
                     NAFSLIMTTELHKLMRADAKQRQFILTRSTYAGQQRAGAVTWSGDIGAAWDIYKHQIA
                     AGLNHSMSGVPYWTFDIGAFVLGAYDGVFCNGGKDPAYQELYTRMFQLGVFTPIFRSH
                     GSETPREIWEFGEFTPTLIKFDNLRYRLMPYIYSQAWKITNEDYTIMRGLAMDYTDDK
                     RVYNIDDQFMFGPSIMACPVTEYQKYTPPLRSVTIPNTVFKTAEGNQGLHAQFFKDKA
                     YKNLGADTIVSNIDVFWYTGRPSYVTDSMFSIRWKGTINPTETGPYQFHLQSFDAKRI
                     RINGKQLKMVYTSVEQYTEVIQLEAGKSYTFELETENTSTGAARMVLKWKTPSLFAAE
                     EVKETRKTTREVYLPKGTQWVDFWTGKTYNGGTSITADARIETMPLFVPAGSIIPMGP
                     FVQYATEKSYDNLEIRIYKGADGSFTLYEDENDGYNYEKGVFSTIDFTWNNKTSELSI
                     GARKGTFPGMLQTRKFSIVLVSEVTGTGIETAKTITKMVDYNGEKIVIKL"
     gene            1764..1837
                     /locus_tag="P5243_04490"
     tRNA            1764..1837
                     /locus_tag="P5243_04490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1798..1800,aa:Ile,seq:cat)
     assembly_gap    1981..2080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2289..2984)
                     /locus_tag="P5243_04495"
     CDS             complement(2289..2984)
                     /locus_tag="P5243_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS18740.1"
                     /translation="MDTSGVKILIVDDEDDIIEILQYNLTKAGYAVYTATDGREAIKK
                     AKKVLPDIILLDVMMPHIDGIQACEDIRQIPELEHCIIAFLTARGEDYSEIAGLQAGA
                     DDYITKPIRPKVLIARLQTLLKRKNNTKQIQASKKIEFEDIVIDEEKHLVFFKNKEIN
                     LPNKEFKLLMLLASKPDRVFTRDEIYTTVWGTDIVVGDRTIDVHIRKLREKLNEEYID
                     TIKGIGYTFIKRI"
     gene            complement(2995..>3180)
                     /locus_tag="P5243_04500"
     CDS             complement(2995..>3180)
                     /locus_tag="P5243_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18741.1"
                     /translation="SRIFERFYRVDKSRSREQGGTGLGLSIVKHIIEAHGQTISVKST
                     INKGTSFTFTIEKAILQ"
BASE COUNT         1001 a          571 c          539 g          969 t
ORIGIN      
        1 gttttggatt agacggttgg tggatagaca gtaccgaacc tgatgttacc aatgcattaa
       61 caaaagaatc taccgactac gaaatgaaga ataaagtagg gaaaaatcat cttggttcgt
      121 gggatagata tctcaatgcg ttttcgctca taatgacaac cgaattgcac aaacttatgc
      181 gtgctgatgc taagcaacga caatttattc ttactcgttc aacttatgcc ggacaacaac
      241 gtgccggagc ggtaacctgg agtggcgata taggtgctgc ctgggatata tacaaacatc
      301 aaattgcagc aggattaaat cattctatgt cgggtgtacc atactggaca tttgatatag
      361 gtgcatttgt acttggtgca tacgatggag tgttttgcaa tggtggcaaa gaccctgcat
      421 atcaagaact ctatacccgt atgtttcaat taggtgtatt tactcctatt ttccgttcac
      481 atggttccga aactccacgc gaaatatggg aatttggaga atttactcct actcttataa
      541 aatttgataa tttacgatac cgattgatgc catatattta ttcgcaagca tggaaaatta
      601 ctaacgaaga ttatacaatc atgcgaggat tggctatgga ttataccgac gacaaacgcg
      661 tgtataatat tgacgaccaa tttatgtttg gcccatcaat tatggcgtgt ccggtaactg
      721 aatatcaaaa atatacaccg ccattacgca gtgtaacaat tccaaatact gtatttaaaa
      781 cagcagaagg caaccaagga ttacatgcac aatttttcaa agacaaagca tacaaaaact
      841 taggtgccga caccatagtt tcaaatatag atgtgttttg gtataccggc cgtccttcgt
      901 atgtaaccga ttctatgttt tctattcgtt ggaaaggcac tataaaccct accgaaaccg
      961 gtccgtatca atttcattta caatcgtttg atgccaaacg aattcgcata aacggcaaac
     1021 aattgaagat ggtatatacc agtgtagaac aatataccga agtaattcag ttagaagcag
     1081 gtaaatcgta tacgtttgaa ttagaaactg aaaatacatc gaccggagca gctcgtatgg
     1141 ttttgaaatg gaaaacgccg agtttgtttg ctgccgaaga agttaaagaa actcgtaaaa
     1201 caacacgcga ggtatattta cccaaaggca cacaatgggt tgacttttgg acaggcaaaa
     1261 cttacaatgg cggcacaagc ataactgcag atgcccgcat agaaactatg cctttgtttg
     1321 tacctgcagg ttctattatt cctatgggac cgtttgtaca atatgctaca gaaaaatcgt
     1381 atgacaattt agaaatacgc atttataaag gagccgatgg ttcgtttacc ctttacgaag
     1441 acgaaaacga tggatataat tacgaaaaag gagtgttttc aacaatcgat ttcacatgga
     1501 acaataaaac gagtgaatta tcaattggtg ctcgtaaagg tacttttccc ggaatgttgc
     1561 aaacacgtaa attttcaatt gtattagttt cagaagttac cggcaccgga atagaaacag
     1621 caaaaactat tacaaaaatg gttgattata atggcgaaaa aattgtaata aaactgtaat
     1681 tccaaaaaaa taatttacaa atacttgttt taatacaaca attatgtatt tttgcaaacc
     1741 caaaataaca aatatgttat ttcgggccca tagctcagct ggttagagca cctgactcat
     1801 aatcaggggg tcgcaggttc aagccctgct gggcccacta taaaagaaga cttacattct
     1861 attgtgagtc tttttttttt caacataact cagctgatta gtgcatcccg atacatatcg
     1921 ggagcgtcgc aggttcaagc cctgctgggc ccactataaa agaagactta cattctattg
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgagtctttt tttttgaaca
     2101 tactgctgcc ggtgaaagca tcgtgatgta ccactaacat gtgaaagaat atagctcaga
     2161 aatttgatta gtatctataa aaaagcctgt aaggctgaca tttttataga aaaatatttt
     2221 atgattctta aaacaccaaa tattaaaatt tatttagcat aagaaaaact aacactacct
     2281 attatacatt agattcgttt aataaaagta taacctatac cttttatagt atctatatat
     2341 tcttcgttta gtttttcacg aagttttcga atatgcacat caatcgttct atcaccaaca
     2401 acaatatctg ttccccaaac ggtagtataa atttcgtcgc gagtaaaaac cctatcgggt
     2461 ttagatgcaa gtaacattaa cagtttaaat tctttatttg gcaaattgat ttctttattt
     2521 ttaaagaaaa ccaaatgttt ttcttcatca attacaatat cttcaaactc aattttttta
     2581 gaggcttgaa tttgtttcgt attatttttt cgtttgagta aagtctgcaa tctggctata
     2641 agcaccttag gccgaatagg tttagtaata taatcatctg ctccggcttg taacccggca
     2701 atttctgaat aatcttcacc ccgagctgtt aaaaaagcaa taatacaatg ttccaattcg
     2761 ggaatttgac gtatatcttc acatgcttga attccgtcta tatgaggcat cattacatcg
     2821 agcaaaataa tatcgggcaa tacttttttt gcttttttta ttgcttcacg accatcggtt
     2881 gcagtatata cagcatatcc ggcttttgtt aaattgtact gtaaaatttc aataatatcg
     2941 tcttcatcat caacgatgag aatttttact ccagatgtat ccatatgtca aaatttattg
     3001 caatatagct ttttctattg taaatgtaaa cgatgttcct ttatttattg tgctttttac
     3061 gctaattgtt tgaccatgag cctctattat atgctttact attgacaacc ccaacccggt
     3121 tcctccttgt tctcgcgaac gacttttatc aactctataa aatcgttcaa atattctgct
//
LOCUS       DAOTOW010000196         3176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23951_length_3176_cov_3.660365, whole genome shotgun sequence.
ACCESSION   DAOTOW010000196 DAOTOW010000000
VERSION     DAOTOW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23951_length_3176_cov_3.660365"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="P5243_04505"
     CDS             <1..573
                     /locus_tag="P5243_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRS18742.1"
                     /translation="ILNVEKAMTHKIYESEEISNILKALGISIGTEEDSKAINIEKLR
                     YHKIIIMCDADIDGSHIETLIMTLFFRYMKEIIERGHLYIATPPLYLVKSKRSKQERY
                     CWSEEERLATIEEFGEGNVTIQRYKGLGEMNASQLWETTMDPERRTLRQVTIESSAEA
                     DHIFSMLMGDDVAPRRAFIEANAKYANIDA"
     gene            660..1781
                     /gene="dnaN"
                     /locus_tag="P5243_04510"
     CDS             660..1781
                     /gene="dnaN"
                     /locus_tag="P5243_04510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRS18743.1"
                     /translation="MEFIVSTSDLLSQLNIVKGVINSKNTLPILDNFLFKLEGNTLKI
                     AASDLETTLNTQMELKNAKGKGIIAIEAKRLTDLLKEFTDQPLTFKINQENYQIDILS
                     ASGKFTIVGQNGDEFPKLPQLEEEFSSISIQSEYFQKGITSTLFATGDDEMRPVMNGI
                     LVELTDKHFRLVATDSHKLARYTRLDVKADKAASFILPKKTANILKNILAKDGNTITI
                     EFDNKNAFFNLTDYKLVCRLIEGKYPAYQAVIPTQNPNKLLVDRIDFFNKLKVVSLFA
                     NPASNLAKLEMGGSTLLISAQDIDFSTSGQEQLQCRYEGEEMVIGFKSKFLLDILQNI
                     NSVEVSIELSDPSRAGIIIPIDKEDANEDILMLLMPMMV"
     gene            1843..2616
                     /locus_tag="P5243_04515"
     CDS             1843..2616
                     /locus_tag="P5243_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRS18744.1"
                     /translation="MTQLQLSRPLAFFDVETTGINIVTDRIVEIAILKVMPNGSELTA
                     TYRINPEMPIPIKASEIHGIYDADVADKPTFAQVGKEIVQFLNDCDIAGYNSNKFDIP
                     ILAEEFARAGIEFNWDKRKFVDVHVLFLKKEARTLAAAYQFYCNKDLENAHSADADTR
                     ATYEVLLAQLERYTDIKNDIQFLSEFTSHTNNVDFAGRIIYDDKKQEVFNFGKYKGML
                     VREVFKKDPNYYTWMMNGEFHSHTKQVITAIKLRDFNTK"
     gene            2682..>3176
                     /locus_tag="P5243_04520"
     CDS             2682..>3176
                     /locus_tag="P5243_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRS18745.1"
                     /translation="MKIVAIEESFNNSVCVCYPDSSLLRNNDDFYIPNFSSHIQATIG
                     VYISIHKIGKHIDTQFIHRYYKSYGLAINFVATDVIQQLHNKHLSTDMARGFDHSFAV
                     STHVFSKDSHPFEKAHFECTYNTMHFESKGAQIIDKIHAICAECSQYYTLKIGDIVFL
                     PLWNI"
BASE COUNT         1139 a          423 c          543 g         1071 t
ORIGIN      
        1 attttgaatg ttgaaaaagc tatgacacat aaaatatatg agagtgaaga aataagtaat
       61 attttaaaag ctttaggcat ttcaataggt actgaagagg acagtaaagc aataaatatt
      121 gaaaaattac gatatcataa aattataatc atgtgtgatg ccgatattga tggtagtcac
      181 attgaaactc ttattatgac attgtttttt agatatatga aagaaattat tgaacgtgga
      241 catttgtata ttgctacacc acctttgtat ttggtaaaat ctaaacgaag taagcaggaa
      301 cgttattgct ggagtgaaga agaacgactt gcaacaattg aagagtttgg agaaggaaat
      361 gttactattc agcgatacaa aggtttggga gaaatgaatg cctctcaatt gtgggaaaca
      421 acaatggacc ccgaaagaag aaccttgcgt caggtaacaa ttgaaagtag tgccgaagca
      481 gatcatatat tctctatgct tatgggtgat gatgttgctc cacggcgtgc atttattgaa
      541 gctaatgcta aatatgcaaa cattgatgcg taatcaagta actaaaattg cataaagcat
      601 acagagcatg ttattttttt ttgtaatttt gtataatatc aaaatgtaag atttttatta
      661 tggaatttat agtatcaacc tcagatcttt tgagtcaatt aaatattgta aaaggtgtta
      721 ttaactcaaa aaacacatta cctatattag ataatttttt atttaaatta gaagggaaca
      781 ctcttaaaat agctgcttca gaccttgaaa caacattaaa tactcaaatg gagttaaaaa
      841 atgcaaaagg taaaggtatt attgctattg aggcaaaacg gttaactgat ttgcttaaag
      901 aatttaccga tcaaccctta acatttaaaa ttaatcaaga aaattatcaa attgatatac
      961 tttcggcaag tggtaaattt acaattgttg gtcagaatgg tgacgaattt cctaaattac
     1021 cacaacttga agaggagttt tcatctattt ctattcagtc cgaatatttt caaaaaggta
     1081 ttacaagtac cttgtttgct accggcgacg atgaaatgcg acctgtaatg aatggtattt
     1141 tggtagaatt aaccgataaa cattttagac ttgttgccac tgattctcat aaattggctc
     1201 gctatacgcg attagacgta aaagcagata aggctgcatc gtttattctt cctaaaaaaa
     1261 cagcaaatat tttaaaaaat attttagcaa aagatggtaa taccataaca atagaatttg
     1321 ataataaaaa tgcatttttt aatcttaccg attataaatt ggtatgtcgt ttaattgagg
     1381 gtaaatatcc tgcttatcaa gcagttattc ctactcaaaa tcctaataaa ttattggtag
     1441 acagaataga tttcttcaat aaattaaaag tagtgtcttt atttgctaat cctgcaagta
     1501 acttggcaaa attagaaatg ggtggttcta cgttacttat ttcagctcag gatatagact
     1561 tctctacttc aggtcaggaa caattacaat gtcgttatga aggtgaagaa atggtaattg
     1621 gttttaaatc taaattctta cttgatattt tacaaaatat taatagtgtt gaagtaagta
     1681 ttgaactttc tgatccttcg cgagctggta ttataatacc tattgataaa gaagatgcaa
     1741 atgaagatat tcttatgctt ttaatgccaa tgatggtata ataacatttg tttttaaaca
     1801 tgaagggaga gtttattctc cctttttttt tattcatata atatgactca attacaatta
     1861 tcacgtccac tcgcattttt tgatgtagaa actaccggta ttaatatagt aaccgataga
     1921 attgtagaaa ttgcaatttt aaaagttatg cctaatggga gtgaacttac tgcaacatac
     1981 agaattaatc ctgaaatgcc aattcctata aaagcatcag aaattcatgg tatatatgat
     2041 gccgatgttg ccgataaacc tacatttgct caggtgggta aagaaattgt tcagttcctt
     2101 aatgattgcg atattgccgg atacaattca aataaatttg atattcctat attagctgaa
     2161 gagtttgctc gtgcaggtat tgaatttaat tgggataaac gaaagtttgt tgatgtgcat
     2221 gtgttatttc ttaaaaaaga agctcgaact cttgctgcag catatcaatt ttattgtaac
     2281 aaagatttag aaaatgcgca tagtgctgat gctgatactc gtgcaaccta cgaagtttta
     2341 cttgctcaat tggaacgata tactgatata aaaaacgata ttcagttttt aagtgagttt
     2401 acctcacata ccaataatgt tgattttgcc ggtagaataa tttatgacga taaaaaacaa
     2461 gaagtgttta attttggcaa atataaaggt atgcttgtga gagaagtgtt taaaaaagat
     2521 ccgaattatt atacatggat gatgaacgga gaatttcatt cgcatacaaa acaagtaatt
     2581 actgctatta aattacgtga ttttaataca aaataatagt tgcactgtat agttatttac
     2641 atgttgggtg aataaaaaaa aaacaggaaa aattatatag aatgaaaata gttgcaatag
     2701 aagaatcatt taataattct gtgtgtgttt gttatcccga ttcaagtttg ttgcgcaata
     2761 atgatgattt ttatatccct aattttagtt cacatattca agcaacaatt ggagtatata
     2821 tatcaattca taaaataggg aaacatattg atacacaatt tattcatcgt tactataaat
     2881 cgtatggttt agcaataaat tttgtggcga cagatgttat acaacagttg cacaataaac
     2941 atctttctac tgatatggca cgtggttttg accattcatt tgcagttagc actcatgttt
     3001 tttctaaaga ttcacatcca tttgaaaaag ctcattttga atgcacatat aatactatgc
     3061 attttgaaag taaaggtgct caaattattg ataaaattca tgcaatatgt gcagaatgta
     3121 gtcaatatta tactttaaaa ataggtgata ttgtgttttt accgctatgg aatatt
//
LOCUS       DAOTOW010000197         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_23968_length_3174_cov_3.040398, whole genome shotgun sequence.
ACCESSION   DAOTOW010000197 DAOTOW010000000
VERSION     DAOTOW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23968_length_3174_cov_3.040398"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="P5243_04525"
     CDS             complement(<1..489)
                     /locus_tag="P5243_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRS18746.1"
                     /translation="MKQKTVAYYTLGCKLNYAETDAIARTMESHGYTRVKFSEKADYY
                     IINTCSVTEAANKKSRYMIRKVYTRSPNATIIIVGCYAQLKPHEISSIAGVSLVLGAT
                     DKFNIPTKIAEYEAAGSPQLVSSCEISEVFLFQSSYSLEERTRSFLKVQDGCDYNCSY
                     CTI"
     gene            complement(523..2247)
                     /locus_tag="P5243_04530"
     CDS             complement(523..2247)
                     /locus_tag="P5243_04530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRS18747.1"
                     /translation="MNTVFEQAKQKRTFVSPTISIDSWQSIQPYFEYLQNYEITDVDS
                     TIQWMLKRSELEAVLEEDLAWRYIKMNCNTQDQKLAQSFQLFVSQIQPEVQKASNSLD
                     KKILASPYISQLPKEYTIALRDIANRESLFCEANVPIIAELQQQEQEYGVINSKMTVH
                     IDGQEKTLQQAAVYLKSTDRALRKQVFEQISERRMLDAEALQKLLTDLITKRHTIACN
                     AGYQNYRDYKLQELGRFDYTKEDCFAFHNSIRACVPPIVKEIQRKRLATLGYESLKPW
                     DTEVDTDNKPALVPFTSIDDFIRKSVACFNSLDPSYGEYLQTMYSQGYMDLESRVGKA
                     PGGFNYPLYESNMPFIYMNAAGTIRDVETLMHEGGHALHSFFSSHLPLVEFKSLPSEV
                     AELASMSMELLSMEHWDVFFDNAEDVKRAKISQLEGIISILSWIAIVDEFQHRLYENP
                     YHTIEERTHIWMQLLESHETHMIDWQGWEHVRAVSWQKQLHIYEVPFYYIEYGIAQLG
                     AIAIWKNYKHNPKETIKQYENMLRTGYTCTIPKIYAKAGIQFSFTQEYVSDLLQYVYS
                     ELQVLYSY"
     gene            complement(2247..2807)
                     /locus_tag="P5243_04535"
     CDS             complement(2247..2807)
                     /locus_tag="P5243_04535"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRS18748.1"
                     /translation="MLKDILAISGYPGLYKLVAQNQKSIIVESLETKKRIPVYQTTKV
                     SALEDIAIYTTDEEVPLVSIFEKIYTIENKGMCSVQKNASNNDIKEYFEDVLPDYDKD
                     RVYISDMKKVLNWYAILHAQNMLQFDAKAKETSNESSETKEKTLDEVKPKAVAKAPKK
                     TVVKQTAAPKNVKAPAAQKKSVPVKK"
     gene            complement(2836..>3174)
                     /locus_tag="P5243_04540"
     CDS             complement(2836..>3174)
                     /locus_tag="P5243_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRS18749.1"
                     /translation="HFLYKIGLSDTVLDASEMRVIDDISKYLGISAGDANSIRATFYD
                     DLESAYQALEINANATDEEVKKAYKKMAVKYHPDKVSNMGEEVQKSATEKFKKINEAF
                     DRIKKQRGIK"
BASE COUNT          977 a          583 c          452 g         1162 t
ORIGIN      
        1 aattgtacaa tacgaacaat tatagtcaca tccgtcttga actttcaaaa aagaacgtgt
       61 gcgttcttct aacgaatatg aactttgaaa caaaaaaacc tcagaaattt cacaagaaga
      121 tactaattgt ggcgaaccag cagcttcata ctctgcaatt ttagtaggaa tattaaactt
      181 atctgtagct cctaaaacaa gtgaaacccc tgctatagat gatatttcat gaggttttaa
      241 ttgagcatag caaccaacaa ttataattgt agcgtttgga ctacgagtat atactttgcg
      301 aatcatatac ctgctttttt tattggcagc ttctgtcaca ctacaggtat taataatata
      361 ataatcggcc ttttcactaa atttcacacg agtataccca tgagattcca ttgtacgcgc
      421 tatggcatcg gtttcggcat aatttaattt acaacctaat gtataatatg ctactgtttt
      481 ttgtttcatt acacttaaat ctcattacag gtattcaatc ttctaataag aatacaaaac
      541 ttgtaattcg ctatacacat attgtaataa atcacttaca tattcttgtg taaacgaaaa
      601 ttgaattcct gctttagcat atatttttgg tatagtacac gtataacctg ttcgtagcat
      661 attttcatat tgcttaatag tttctttggg attatgttta taatttttcc atattgctat
      721 agcacctaat tgagcaatac catattctat ataataaaaa ggcacctcat aaatatgcaa
      781 ttgtttttgc cacgacactg cacgtacatg ttcccaccct tgccaatcta tcatatgtgt
      841 ttcatgcgac tctaacaact gcatccatat gtgtgtacgt tcttctatag tatgatacgg
      901 attttcatat aaccgatgtt gaaattcatc aactattgca atccatgaca aaatagatat
      961 aataccttct aattgactga tttttgctcg ttttacatct tcagcattat caaaaaacac
     1021 gtcccaatgt tccattgaaa gtaattccat actcatagat gcaagttctg caacctccga
     1081 tggcaaactt ttaaattcca ctaatggtaa atgcgaagaa aagaatgaat gcaacgcatg
     1141 acctccttcg tgcataagtg tttccacatc acgaatagtt cctgcagcat tcatatatat
     1201 aaaaggcata ttactttcat acaaaggata attgaatcca ccgggagctt taccaactcg
     1261 tgattctaaa tccatatacc cctgagaata catagtttgc aaatattctc catacgaagg
     1321 gtctagagaa ttaaaacaag caaccgattt acgtataaaa tcatcaattg aagtaaaagg
     1381 aaccaatgcc ggtttattat ctgtgtcaac ttcggtatcc caaggtttaa gcgattcata
     1441 tcctaatgta gcaagtcgtt ttcgttgaat ttccttgact ataggcggca cgcatgcccg
     1501 tattgaatta tgaaatgcaa aacaatcttc ttttgtataa tcaaaacgac ctaattcttg
     1561 caatttataa tcacgataat tctgatatcc tgcattacac gcaatagtat gtcgttttgt
     1621 aattaaatct gttaatagtt tttgaagagc ttcggcatca agcattctgc gttccgaaat
     1681 ttgttcaaaa acttgttttc gtaaagctct atcagtactt ttaagatata cagctgcttg
     1741 ttgaagtgtt ttttcttgcc catcaatatg aacagtcatt ttcgaattaa taactccata
     1801 ttcttgttct tgttgttgca attcagcaat tatgggcaca tttgcttcac aaaataaact
     1861 ttcacgattt gctatatcac gtaaagcaat agtgtattct ttgggtaatt gagaaatata
     1921 tggtgatgcc aatatttttt tatccaaact atttgaagcc ttttgaacct caggttgaat
     1981 ttgtgaaaca aacaattgaa atgattgagc aagtttttga tcttgcgtat tacagttcat
     2041 ttttatatac cgccatgcca aatcttcttc gagcacagct tcaagttcac tacgtttgag
     2101 catccactgt atagttgaat caacatcagt tatttcatag ttttgtaaat attcaaaata
     2161 tggttgaata ctctgccatg aatctatact aatagtaggc gaaacaaatg ttcttttttg
     2221 cttagcttgt tcaaatacag tattcattat tttttaacgg gaaccgattt tttttgagct
     2281 gccggagctt ttacattttt aggagcagca gtctgtttta caactgtttt tttaggtgct
     2341 tttgcaacag cttttggttt tacttcatca agtgtttttt ctttagtttc cgatgattca
     2401 tttgatgttt ctttggcttt tgcatcaaat tgcaacatat tttgagcatg taaaattgca
     2461 taccaattca aaactttttt catatcagat atatacactc tgtccttatc ataatcaggc
     2521 aatacatctt caaaatattc tttaatatcg ttgtttgagg catttttttg aacagaacac
     2581 atacctttat tttcaattgt gtatattttt tcaaatatag aaacaagagg cacctcttca
     2641 tcagtagtat aaatagcaat atcttctaac gcactcactt ttgttgtttg atacaccggt
     2701 attcgctttt tggtttcaag cgattctaca ataatacttt tttgattttg agctacaagt
     2761 ttatacaaac ccggataccc tgaaattgct aaaatgtcct taagcatacg tattgattta
     2821 aattaatatt tataattatt ttattcctcg ttgttttttt attctatcaa aagcttcatt
     2881 tatcttttta aatttttctg tagcagattt ttgcacttct tcacccatat tacttacttt
     2941 atcaggatga tacttaactg ccattttttt gtatgctttt tttacttctt catcggttgc
     3001 gtttgcattt atttctaagg cctgataagc actttccaaa tcatcgtaaa acgttgcacg
     3061 aattgaattt gcatcacctg ctgatattcc caaatattta gaaatatcgt ctatcactcg
     3121 catttccgaa gcatctaata cagtatcgga taatccaatt ttataaagaa aatg
//
LOCUS       DAOTOW010000198         3169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24012_length_3169_cov_4.132948, whole genome shotgun sequence.
ACCESSION   DAOTOW010000198 DAOTOW010000000
VERSION     DAOTOW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24012_length_3169_cov_4.132948"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5243_04545"
     CDS             <1..478
                     /locus_tag="P5243_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS18750.1"
                     /translation="KILEVGSNTGMQLAGLQRMGFTNLYGIELQWYAVEKAKEYTKGI
                     NIIQGSGFDIPFKDGYFDLVITNGVLIHIAPKDLPAIMSEIVRCSKQYIAGFEYYAEE
                     CTDINYRGNTGFLWKMDYEAKYRELFPQLKPVYKQLYPYLNSQEQGNKDCMYLLEK"
     gene            483..1169
                     /gene="pseF"
                     /locus_tag="P5243_04550"
     CDS             483..1169
                     /gene="pseF"
                     /locus_tag="P5243_04550"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HRS18751.1"
                     /translation="MNCIAIIPARGGSKRIPRKNIKPFHGKPIIAYSIEAALQSGLFT
                     EVMVSTDDEEIAEIAKSYGAKVPFYRSAETANDFAHLAQVIEEVFAMYAQQGKTFDTF
                     CCILPTAPFISHNRIIEAYHVLTEKHCDSVFAVVQYSYPIQRALYIRQNKVSMCNPEH
                     LLTRSQDLEPTYHDSGQFYWCNTQQFITNKVFLSNNTGAVVLSAMEVQDIDSEEDWKI
                     AEFKYKAYKI"
     gene            complement(1170..2537)
                     /gene="radA"
                     /locus_tag="P5243_04555"
     CDS             complement(1170..2537)
                     /gene="radA"
                     /locus_tag="P5243_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRS18752.1"
                     /translation="MAKSKTIYVCQSCGAQSPKWIGKCNSCNNWNTFQEEIITSSVAS
                     KSNSPSVTTKPIVLTEIKSENHTRIDTGIHELNQVIGGGIVPGSVILLGGEPGIGKST
                     LLLQVAVALTKKTLYVSGEESPQQISLRAKRLNMHKDSCIIYSETDVQAILHQIETLQ
                     PELVIVDSVQTLKHPDIDSSPGSVSQIRETTNVLIEVAKKTHIPIILIGHITKEGSIA
                     GPKILEHMVDVVLQFEGDMHNVYRILRSNKNRFGSTSEIGIFEMVQSGLREVSNPSEM
                     LLSGNPEQLSGVAVASVMEGVRPLLIEVQALVSTAAYGTPQRSVNGYDAKRLNMLLAV
                     LEKRLGVKMYTKDVFVNIAGGIKVQDTAIDLAVVAAILSSLFDVYIPASYCFAGEIGL
                     TGEVRMVGHLEKRIKECSKLGIQQMFIPKHDVHVQSKHTNIVHCTRIDTTFRALFKPD
                     SKVNE"
     gene            complement(2537..2938)
                     /locus_tag="P5243_04560"
     CDS             complement(2537..2938)
                     /locus_tag="P5243_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18753.1"
                     /translation="MKFKFFLYALAFIFTFTMCSKRTEVKIIGEWQVVNVGQDNFPDD
                     ATWTFNDGGKLDIFHDINGDPVGTVTGDWEAFARNAVIPYIEIKGLGPKGMDGKWRVE
                     RLNKKFLILQRVEFGNGETAGAFLRREFQKK"
     gene            complement(2985..>3169)
                     /locus_tag="P5243_04565"
     CDS             complement(2985..>3169)
                     /locus_tag="P5243_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRS18754.1"
                     /translation="FAQETPYELITAIIPNILVKGSDYNENTIVGADVVKKHGGKVTT
                     IDFVSGYSSTNYFKKM"
BASE COUNT         1018 a          622 c          542 g          987 t
ORIGIN      
        1 aaaaatattg gaagtaggat ccaacaccgg catgcagttg gcaggcttac aacgtatggg
       61 attcaccaat ttgtatggca tagaattgca atggtatgca gtagaaaaag caaaagaata
      121 taccaaaggc ataaacataa ttcagggttc aggattcgat attcctttca aagacgggta
      181 ctttgatttg gttataacca atggcgtgct tattcacata gcacccaaag atttacctgc
      241 cataatgagc gaaattgtac gttgttcaaa acaatacatt gcaggttttg aatactatgc
      301 cgaagaatgt accgacataa attacagagg aaatacgggg tttttgtgga aaatggatta
      361 cgaagcaaaa tatcgtgaac tgtttccaca gctaaaaccg gtgtacaaac aattgtatcc
      421 gtacctcaat agtcaagagc aaggcaacaa agattgtatg tatttattgg aaaaataata
      481 ccatgaattg tatagctata ataccggcac gaggcggcag caaacgtatt ccgcgaaaga
      541 acattaaacc ttttcacgga aaacctatta tagcatattc tatagaagct gcactgcaat
      601 cgggtttgtt taccgaagtt atggtatcaa ccgacgatga agaaatagct gaaatagcaa
      661 aatcatacgg tgctaaagta ccattctata gaagtgccga aactgcaaac gattttgcac
      721 atttggctca agtaatagaa gaagtgtttg caatgtatgc acagcaagga aaaacctttg
      781 ataccttttg ttgcatattg cctactgcac catttatttc tcacaaccga attattgagg
      841 cataccacgt acttacagaa aaacactgcg attcggtatt tgccgtggtg caatatagtt
      901 atcctataca acgagcattg tatattcgtc agaataaagt aagcatgtgc aatcccgagc
      961 atttgctaac ccgttcgcaa gatttagaac ctacctacca cgacagtggg caattttatt
     1021 ggtgcaacac ccaacaattt attaccaaca aagtatttct gagcaacaac accggagctg
     1081 tggtactatc ggctatggaa gtgcaagata ttgacagcga agaagattgg aaaatagctg
     1141 aatttaaata taaagcatat aaaatataac tattcgttta cttttgaatc aggcttaaac
     1201 aaagctctaa aggtagtgtc aattcgagta caatgcacta tattggtatg tttgctttgc
     1261 acatgcacat catgttttgg aataaacatt tgttgtatgc ccaatttact gcattctttt
     1321 atacgttttt ctaaatgtcc caccattcgc acctcaccgg taagtcctat ttcacctgca
     1381 aaacaatacg atgcgggtat atacacatca aaaagcgaag aaagtatagc agcaactaca
     1441 gctaagtcta ttgcagtatc ttgaacttta atacctccgg ctatatttac aaatacatct
     1501 tttgtgtaca tttttactcc caaacgcttt tctaatacag caagcaacat attgagtcgt
     1561 ttggcatcgt atccatttac actccgttgt ggtgttccgt atgccgcagt gctcacaagg
     1621 gcttgtacct caataagcaa aggtcttaca ccttccatta ccgatgccac tgctacacca
     1681 cttaattgtt caggattgcc gctaagcagc atttcggaag gatttgaaac ttcacgaagt
     1741 cctgattgaa ccatttcaaa aatacctatt tctgatgttg atccaaacct atttttatta
     1801 cttcgtaaaa ttcgatatac attgtgcata tcgccttcaa attgtaatac cacatctacc
     1861 atatgttcca atatttttgg accggcaata ctgccttctt tagtaatatg tccaattaaa
     1921 attataggta tatgcgtttt tttagcaact tcaatcaaca cattggtagt ttcacgaatt
     1981 tgcgaaacac tccccggcga tgaatctata tcaggatgtt tgagcgtttg caccgaatct
     2041 actataacca attcgggctg caatgtttca atttgatgta atatagcttg cacatcggtt
     2101 tcactataga taatacaaga atctttgtgc atattcaatc gtttggcacg taatgaaatt
     2161 tgttgcggac tttcttcgcc cgaaacatag agcgtttttt ttgtaagagc aacagcaact
     2221 tgcaataata aagtagattt tccaataccg ggttctccac ccaataaaat aaccgaaccc
     2281 ggaactatac ccccacctat tacctgatta agctcatgaa taccggtatc tatacgtgta
     2341 tggttttcag atttaatttc ggttaaaaca ataggtttgg ttgtaaccga aggcgagttt
     2401 gattttgatg caaccgacga tgttataatt tcttcttgaa acgtattcca attgttacaa
     2461 gaattacatt tcccaatcca tttaggcgat tgggctccac atgattgaca aacataaatt
     2521 gttttggatt ttgccactat tttttctgaa attctcgtcg taaaaaggca ccggcagttt
     2581 caccattacc aaattctacg cgttgaagaa taagaaattt tttatttaat cgttcaacac
     2641 gccatttacc gtccatacct ttaggaccaa gtcctttaat ttcaatatat ggtattacag
     2701 cattgcgagc aaatgcttcc cagtcgccgg taactgttcc taccggatca ccgttaatgt
     2761 catgaaaaat atcaagtttt ccgccgtcat taaaagtcca tgtagcatca tcaggaaaat
     2821 tatcttgccc tacatttact acttgccatt ctcctattat ttttacttcg gttcgttttg
     2881 aacacatagt aaaagtaaaa ataaatgcta aggcgtatag aaaaaatttg aacttcatat
     2941 ttttttgaaa attagatttc aaagataaaa aataatttga aatattacat ttttttgaaa
     3001 taatttgttg atgaatatcc gcttacaaaa tctatagtag taactttacc tccatgtttt
     3061 ttaactacat cggccccaac tatggtattc tcattataat cacttccctt tacaagtatg
     3121 tttggaataa tggcagtaat taattcataa ggggtttctt gcgcaaatg
//
LOCUS       DAOTOW010000199         3154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24154_length_3154_cov_2.867699, whole genome shotgun sequence.
ACCESSION   DAOTOW010000199 DAOTOW010000000
VERSION     DAOTOW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24154_length_3154_cov_2.867699"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..2261
                     /locus_tag="P5243_04570"
     CDS             177..2261
                     /locus_tag="P5243_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRS18755.1"
                     /translation="MNRIFRFTTVFLLLVQLGFSQSADWKFRYEYVKNNPQELDVFMD
                     ITIPKDFHMYSFDQGPGGPIAADVQFVVPAGVKKVGSLQSLTKPREYFDDIFGITTRD
                     FSGKVTWKQRFFVGTSDAFIITGKYSYQLCMDDGVCINPLPEKFSISIPAPAVSKKQI
                     DSVKSVVSQVDILLQTTTTDSMKTSDSIKQGTASSEPTSIPSEFDDSTAGMSLWWIFI
                     FGFLGGLLAFLTPCVFPMVPMTVSMFIKRSKDKAVRDVSLYGISIVGIYVVLGLLITV
                     IFGVDALNKMSTSPLFNVVFFAIFLLFAISFFGAFELVLPSSWVNYMDKKADTSSGFA
                     SIFFMAFTLVLVSFSCTAMIIGTLLVQSVVSGSLLGPFMGMLGFSIALALPFVIFAFV
                     PSLITSIGKSGGWLQTVKVTLGFIELGFALKFISKADLVAGWGILPRDLFLAIWIILL
                     VMLGLYILKKIKLSHDDDSTTVSTPRFMLAMAIFSFALYLVPGMFGAPLKSLSGYLPP
                     MSTQSLDLYTPTLYSQSSSSATSSTIKQAKKYADEFHCPHNIDCFFDYEEGMAYAKQV
                     NKPVLLDFTGKGCENCRKIEMNVWSNPQVLKMLHDDYVVISLYVDARTPLEKELVRTE
                     QYGGKEYDITTVGEHWSMFMMQNYGGLMQPYYVLLSPDGKRLAKPITYTEAQSIEFYT
                     KFLQGGIEAMKK"
     gene            2411..>3154
                     /locus_tag="P5243_04575"
     CDS             2411..>3154
                     /locus_tag="P5243_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18756.1"
                     /translation="MIKIKNKYIFFLIVLFPFIFFTIVFFIVRTKEINIITDSNFSIS
                     EFTDASNNGNSISIIKKQKDTLFIQNILKKGYEYPYSGMQINKKNYSLFSLYDYTFKL
                     KIHVNSDVRISFRISEYLDSYTDTLKPLSYLILVKSLGLKKGFNEIILNSNQITDVQD
                     WWFQKNPSQINNLNLSNNLKKIKAIWFFIENTTPIDQNIEFKISEFKLAYNPLSLIFT
                     FLYITIVYFIIVFFVIWKLKKVKYIFLPID"
BASE COUNT         1014 a          459 c          541 g         1140 t
ORIGIN      
        1 gtaactgagt tcttttgcca tatgtatatt tttagatagt gatactaaag cgatatatga
       61 tatgtttttt tatgatgtat ttataccata tttatataga caaattataa ttgttgtatt
      121 acttttacgt tcacattcgt ctatagaaat atatatttac aacaaaagta cacaatatga
      181 atagaatttt tcgctttact acagtatttt tattacttgt acaattagga ttttcgcaat
      241 cggccgattg gaaattcaga tatgaatatg taaaaaataa tccgcaagaa ttagatgttt
      301 ttatggatat taccattccc aaagacttcc acatgtatag tttcgatcaa ggtcccggtg
      361 ggcccattgc tgccgatgta cagtttgtgg tgccggcagg tgtaaaaaaa gttggtagct
      421 tacaatcgct caccaaacca cgtgaatatt ttgacgatat ttttggtata acaacacgcg
      481 attttagtgg aaaggtaacg tggaaacaac gtttttttgt aggtacttca gatgcattca
      541 tcattacagg taaatacagc tatcaactct gcatggacga tggtgtgtgt ataaacccat
      601 tacccgaaaa attttctata tcaatacctg cacctgcggt atctaaaaaa caaatagatt
      661 ctgtcaaatc tgttgtatcg caggtagata ttctgttgca gactacgact accgattcta
      721 tgaaaacttc cgattctatt aagcaaggaa cagcttcatc agaacctact tcaattcctt
      781 cggaatttga tgattctacg gcaggcatgt cgctatggtg gatttttatt tttggatttc
      841 tgggtggatt attagcattt ttaaccccct gtgtgtttcc tatggttcct atgaccgtga
      901 gtatgtttat aaaacgcagt aaagacaaag cagtgcgcga cgtgtcgtta tatggaattt
      961 ctattgtagg tatttatgtt gttttaggct tgctaattac agtaatattt ggtgtagatg
     1021 ctttaaataa aatgtctaca agtccactgt ttaatgttgt atttttcgct attttcttgt
     1081 tgtttgccat ttcatttttt ggagcattcg aacttgtatt accatcgagt tgggtaaatt
     1141 atatggacaa aaaagctgat acaagttcag gttttgcaag tatattcttc atggcattta
     1201 cactcgtatt ggtatcgttt tcgtgtactg ccatgattat aggtacgctt ttggtacaat
     1261 cggttgtttc gggctcgttg cttggaccat ttatgggaat gttaggattt tctatagcgt
     1321 tggccctgcc gtttgttatt tttgcatttg ttccatcgct tattacaagt attggtaaat
     1381 cgggtggttg gttgcaaacg gtaaaagtta ccttaggttt tatagaatta ggatttgcgc
     1441 ttaaatttat atcaaaagcc gatttagttg caggttgggg tattcttcct cgcgatttgt
     1501 ttttggctat atggattata ttattagtaa tgttgggctt atatattttg aagaaaataa
     1561 aattatcgca cgacgatgat tccactactg tatctacgcc acgatttatg cttgctatgg
     1621 caatattttc gtttgcactt tatttagtac ccggcatgtt tggagcaccg cttaaatcgt
     1681 tgagcggata cttacctcct atgtcaacac aatcgctcga tttatatact ccaacattgt
     1741 attcacaatc aagcagttcg gcaacatcat ctacaataaa acaagcaaaa aaatatgccg
     1801 atgagtttca ttgcccacac aatatcgatt gcttttttga ctatgaagaa ggaatggcat
     1861 atgcaaaaca agtaaacaaa ccggtattgt tggattttac cggcaaaggt tgtgaaaatt
     1921 gtcgcaaaat agagatgaac gtatggtcga acccacaggt tttgaaaatg ctgcacgatg
     1981 actatgtggt aatatcgttg tatgtagatg cacgcacacc acttgaaaaa gaattggtac
     2041 gaaccgaaca atatggcggc aaagaatatg atataacaac cgtgggtgaa cattggagta
     2101 tgtttatgat gcaaaactac ggaggactta tgcagccgta ttatgtatta ttatcgcccg
     2161 acggcaaacg attggcaaaa ccaataacgt acaccgaagc tcaaagtata gaattttata
     2221 ccaagttttt gcagggaggt atagaggcta tgaagaaata agtttggaag ttctaaaaac
     2281 tttaggcttc taaatcaaaa acaaaattta aacagtttct aagccttaaa ttgcattttt
     2341 aattatattt gttttttagc aataacttaa gaaaatttgg agtaactaat ttttgttaaa
     2401 taacaatttt atgattaaaa ttaaaaataa gtatattttt ttcttaatag tattgttccc
     2461 ttttattttc tttactattg ttttttttat agtaagaact aaagaaatta atattataac
     2521 cgattcaaat ttttctattt ctgaatttac ggatgcttct aataatggaa attcaatttc
     2581 aattatcaaa aaacaaaaag atacattatt tattcaaaat atactaaaaa agggatatga
     2641 atatccttat tccggtatgc aaattaataa aaagaattat tctttatttt cattgtatga
     2701 ttatactttt aaattaaaaa ttcatgtaaa ttctgatgtt cgaatttcat ttagaattag
     2761 tgaatatctt gatagttata ctgacacttt aaaacctctt tcatatttga ttttagtgaa
     2821 gtctcttggt ttaaaaaaag gttttaatga aataatatta aattcaaatc agataacaga
     2881 tgtgcaggat tggtggtttc aaaaaaatcc atcacagatt aataatctta atttaagtaa
     2941 taatttaaaa aaaattaaag caatatggtt ttttattgaa aatactacac cgattgatca
     3001 aaatatagaa tttaagatat ctgagtttaa attggcatat aatcctttgt ctttaatttt
     3061 tactttttta tatattacaa ttgtatattt cataatagta ttttttgtaa tttggaaatt
     3121 aaaaaaagta aaatatatat ttttgccaat cgat
//
LOCUS       DAOTOW010000200         3149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24198_length_3149_cov_2.978022, whole genome shotgun sequence.
ACCESSION   DAOTOW010000200 DAOTOW010000000
VERSION     DAOTOW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24198_length_3149_cov_2.978022"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5243_04580"
     CDS             complement(<1..235)
                     /locus_tag="P5243_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18757.1"
                     /translation="MSNQREILDYLQLHFPSLESALCTEICELGTIETISAGQILMEP
                     GKYIKFLPLLCSGLIEVYRNDAESREVLLYYIKA"
     gene            complement(288..1703)
                     /locus_tag="P5243_04585"
     CDS             complement(288..1703)
                     /locus_tag="P5243_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRS18758.1"
                     /translation="MKSVITFILASVFCISIHAQAQKPKYIFLCIGDGMGLVQAQLAD
                     AYLREQAKDSIPFLYFPYNGLQTTYSLSSKITCSAAAGTALACGVKTKANHIAVDETG
                     VTKLESIAKTLKQRGYKIGIITSVSIDHATPAVFYAHDASRNSYHNIAMQLPVSNFDF
                     FGGGAFVKPVDGKQNAYAELKKYKYSLITSPDSLQYASQLGTKVCVFDKKTELAYGID
                     NMPHRITLAQLMSTAIECVQSPQGFFIMAEGGKIDWACHSNDAATAVKETIEFSKAVQ
                     QAMAWYRKYPNETLIIVTADHETGGMGVGAALYPYYTNISLLANQTYSYVECEAALSA
                     KIKALGSQFTFDSCMAFVTKAYAIGTNNLYLQAYDSVRLQRAYNFVFQSNADASGDGT
                     LLYNISPKALYSSGNKASAIVITMNKIIAEKAGIGWTTYAHTGAMVPVYAIGAGSELF
                     QGLYDNTDIPKKILSLLEITK"
     gene            complement(1881..>3149)
                     /locus_tag="P5243_04590"
     CDS             complement(1881..>3149)
                     /locus_tag="P5243_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRS18759.1"
                     /translation="GSDRLFGAEQKIRIQQQFEEQGIACFFQDGSGITKQTDVVIVST
                     AIEQSNIEYQKALELGIPVMKRSELLAAISNSIKTIAVGGTSGKSTTTAMIFHILQTC
                     GKSPSLITGAGLSELQEKGMPGNAWVGTGEWLVIEADESDGSIVNYKPEISVLLNIDR
                     DHKEYDELIALFTTFKHNTASYFIANQDYELTKQLSDEHAYNFGTQQAPLCGTNFLQE
                     GFEISFTVQSCTCKVPVIGKHNMENALAAIAVAHAIGISIADACGALASFRGIYRRTQ
                     LVGKTENSIYVVDDFAHNPAEVAAAIKACQQIAPHVVAWFQPHGFGPLRFMHAELSEF
                     VSHTLRQTDVFLIADVYYAGGTVDKNISPTIVSDAVALKGKQAVYTGSKEQSLQVIAA
                     TTPPNAVVLIMGARDPYLDAFAVQVLETLR"
BASE COUNT          960 a          646 c          544 g          999 t
ORIGIN      
        1 cagctttaat ataatacaat aatacttcgc gcgattcagc atcgtttcta tatacctcta
       61 taagtccgga gcaaaggagt ggtaagaatt taatgtattt cccgggttcc ataagaattt
      121 gaccggccga aatagtttct atagtaccta attcacaaat ttcggtacat aatgcggatt
      181 ccaacgaggg aaaatgcaac tgtaaatagt caagtatctc tctctgattg ctcatatttg
      241 tttacttgta atatccttta ggctttatac gtataccgta tgcaatccta tttggtaatt
      301 tcaagtaaag atagtatttt ttttggaata tcggtattgt catacaatcc ttggaataat
      361 tcgctaccgg cacctatagc atacacaggt accatagcac cggtatgagc ataggtagtc
      421 cagccaattc ctgctttttc agctatgatt ttattcatgg taataacaat ggccgaagcc
      481 ttattgccgg aactatacaa agccttaggc gatatattgt acaacaatgt accgtcacca
      541 cttgcatctg cattggattg aaatacaaaa ttatatgccc gctgcaatcg tacactgtca
      601 tacgcttgca aatacaaatt attggttcca atagcatagg ctttcgttac aaatgccata
      661 cacgaatcaa acgtaaattg acttcccaat gctttgattt ttgctgatag agctgcttca
      721 cattctacat acgaatatgt ttgatttgca agcaacgaaa tatttgtata atacggatac
      781 aaagctgccc ccactcccat tccacctgtt tcgtggtcgg cagtgacaat aataagggtt
      841 tcgttaggat atttgcgata ccatgccatt gcttgttgta ccgctttcga gaattcaata
      901 gtttccttaa cagcagtagc tgcatcattt gaatggcaag cccaatctat tttaccgcct
      961 tctgccatga taaaaaaacc ttgtggtgat tgcacacatt ctatggccgt agacatcaat
     1021 tgagctaaag taattcgatg tggcatattg tcaataccat acgccaattc tgtttttttg
     1081 tcaaacacac atacctttgt tcccaattgt gatgcatatt gcaagctatc gggtgaggtt
     1141 attaaagaat atttatattt cttgagttcg gcatacgcat tttgttttcc atcaacaggt
     1201 tttacaaatg caccacctcc aaaaaaatcg aaatttgata ccggcaattg catagcaata
     1261 ttgtgataac tattgcgact ggcatcgtga gcataaaaca cggcaggagt agcgtggtca
     1321 attgatacgc tggtaattat tcctatttta tatcctcgtt gttttaacgt ttttgcaata
     1381 ctttcgagtt ttgtaacacc ggtttcatct acagctatat gatttgcttt tgtttttaca
     1441 ccgcatgcaa gtgcagtacc ggcagcagcg gagcatgtaa tttttgagct caatgaatag
     1501 gtagtttgca aaccattgta cggaaaatat aaaaacggaa tactatcctt tgcctgctct
     1561 ctcaaatagg catcagccaa ctgagcctgt accaagccca taccatcgcc tatacacaaa
     1621 aaaatatatt ttggcttttg agcctgagca tgtatagaaa tacaaaagac acttgcaagt
     1681 ataaacgtaa taactgattt cattctaatt ttttttatgt aaacacattg tatatgtgtt
     1741 caaaaataca gctatttttc aactttaaac acagtaatta tattagtttt ttgttaaaaa
     1801 aaccatgtaa tttttagact taactccaaa aattaatgaa aatttttgga gtttgcatgt
     1861 aaaataaaat atcaaaacct ttatctaagt gtttctaata cttgcacggc aaacgcatct
     1921 aaatacgggt cgcgagctcc cataataagc actaccgcat tgggcggcgt agtagctgct
     1981 attacttgca acgattgttc tttggaaccg gtatacaccg cttgttttcc tttgagggca
     2041 acagcatcgc tcacaatagt aggagaaata tttttgtcaa cagtcccgcc ggcatagtat
     2101 acatctgcaa ttaaaaacac atcggtttgg cgtaatgtat gacttacaaa ttccgataat
     2161 tcggcatgca taaatcgcaa cggtccaaaa ccatgaggtt ggaaccatgc aactacatgg
     2221 ggagctattt gctgacatgc tttaatggct gcagctactt cggccggatt atgtgcaaaa
     2281 tcatcaacta catatatact attttcggtt ttaccaacca actgtgttct acgataaatt
     2341 cctcgaaacg aggctaatgc cccacacgca tctgctatag aaataccaat agcatgagca
     2401 acagcaatag ctgccaatgc attttccata ttgtgtttgc caattacagg gactttacag
     2461 gtacatgatt gtacggtaaa agaaatttca aatccttctt gcaaaaaatt ggttccacac
     2521 aaaggagctt gttgcgtacc aaaattatat gcatgttcgt cgctcaactg cttagtaagt
     2581 tcataatctt gatttgcaat aaagtatgat gccgtattat gtttaaatgt agtaaataat
     2641 gctatgagtt catcatattc tttatggtcg cggtcaatat taagcaatac cgaaatttcc
     2701 ggtttataat taacaataga accatcggat tcatcggctt ctatcacaag ccattcacct
     2761 gtaccaaccc atgcattgcc gggcatacct ttttcttgca attccgacaa acctgctccc
     2821 gtaataagcg atggagattt tccgcacgtt tgtaatatat gaaaaatcat agcagttgtg
     2881 gtagacttac ccgatgtgcc tcctaccgca atagttttaa tactatttga aatagctgcc
     2941 aacaattcgg aacgtttcat tacgggtatt ccaagttcca aagctttttg atactctata
     3001 ttactttgtt ctatggctgt agaaactatt acaacatcag tttgttttgt aataccacta
     3061 ccatcttgaa aaaaacaagc tattccttgc tcctcaaact gctgctgaat gcgaattttt
     3121 tgttcggcac caaacaaacg gtcagaacc
//
LOCUS       DAOTOW010000201         3143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24251_length_3143_cov_3.465350, whole genome shotgun sequence.
ACCESSION   DAOTOW010000201 DAOTOW010000000
VERSION     DAOTOW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24251_length_3143_cov_3.465350"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="P5243_04595"
     CDS             <1..769
                     /locus_tag="P5243_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRS18760.1"
                     /translation="RKELDELTEWVKRPQIGAKGLIYIKYNEDGTIKSSVDKFYTPQE
                     LTDICKICNAQIGDLVLIISGEQSLALNALSELRLEMGTRLGLRKKDVFAPLWVIDFP
                     LFEWDEETQRLYAKHHPFTSPKPEDIPLLDTNPKEVRANAYDFVVNGSEIGGGSIRIY
                     NSELQSKMFSLLGFTSEQAQTQFGFLMGAFTYGAPPHGGLAFGFDRFVSILAGADSIR
                     DVIAFPKNNAAKDLMAECPTEISQAQLDELCLQIVKK"
     gene            777..1226
                     /locus_tag="P5243_04600"
     CDS             777..1226
                     /locus_tag="P5243_04600"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRS18761.1"
                     /translation="MSLTEQHSHEYFMKQALQQANIALQKDEVPVGAVIVYNNQIIAR
                     AHNLTETLNDPTAHAEMQAFTSATEYLGGKYLQKATLYVTLEPCAMCGGASYWVQIGT
                     IVYGASDNKRGISALRSHILHPKTQIIGGILQTECETLLQNFFKQKR"
     gene            1239..1874
                     /locus_tag="P5243_04605"
     CDS             1239..1874
                     /locus_tag="P5243_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRS18762.1"
                     /translation="MKHGNTILHIVFGFAIVVLFNLVTINKKTTPGKEKTEPVHIAVQ
                     DSSGNIVIKPFPVAYIIVDSLMRNYLYYKKIEGQYQAMVKQEDAKLQQQGQAFEQEVY
                     QLQSQVQKGLITTRDAQTKEQELQLKQQKLMEWQQSKQQELANKEQLLMKELLDSVQV
                     AIKFHNADNKYEIVLNNAFNSSVLYAKEHLNITDTILKIMNERYSKSLSKK"
     gene            2346..>3143
                     /locus_tag="P5243_04610"
     CDS             2346..>3143
                     /locus_tag="P5243_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS18763.1"
                     /translation="MRISILSFVVYFFLSYCYSQTTANFSLRDDSQYIMCYTEYSNGI
                     AVNNTEVEFINTSSCTGNCRYVFDFGDNTPQIIKDNTSNTTHRYVQDGVFDVRLQVLN
                     TVSIHDSIKNRPFTSVQFISRSDDSVQLAITYTGIDNSRKTATIKIPEGQYARRTLAQ
                     PITVYSPLVESTNFLYEIDDPSTDDSKAPIQSFAHIFTVDTANFKPHDLSKWTYYWEI
                     YKTNEYGEPMNTPYTKFHVDSIEYKYTFPAENFSPGYYVKLKIALDSS"
BASE COUNT         1157 a          459 c          508 g         1019 t
ORIGIN      
        1 acgaaaagaa cttgatgaac ttacagaatg ggtaaagcgt ccgcagattg gagcaaaggg
       61 cttaatttat attaaataca acgaagatgg tactattaaa tcttctgtag ataaatttta
      121 tactcctcaa gaacttactg atatttgcaa aatatgcaat gcgcaaatcg gtgatttagt
      181 attgataata tccggagagc aatcacttgc attaaatgca ttgtctgaat tgcgtttaga
      241 aatgggtact agattgggat tgcgaaaaaa ggatgttttt gcacctctgt gggtaattga
      301 tttcccttta tttgaatggg acgaagaaac acaacgttta tatgcaaaac atcatccttt
      361 tacatcacca aaacccgaag atattccttt gcttgatact aatccaaaag aagtacgtgc
      421 caatgcttat gattttgttg taaatggaag tgaaattgga gggggatcaa ttcgtatata
      481 taattcagaa ttgcaatcaa aaatgttttc attattagga ttcacgtcag agcaagctca
      541 aacacaattt ggatttttga tgggagcatt tacttatggt gctccacctc atggaggttt
      601 ggcatttggt tttgaccgtt ttgtttctat tttggcaggt gccgactcta ttcgtgatgt
      661 aattgctttt cctaaaaata atgctgcaaa agatctcatg gccgagtgtc caactgaaat
      721 ttctcaagcc cagttagatg aattatgctt gcaaatagta aaaaaataaa taattcatgt
      781 cgcttacaga acagcatagc cacgagtatt ttatgaagca agccttgcaa caagcaaata
      841 ttgctttgca aaaagatgaa gttcctgtag gtgcagtaat tgtatataat aatcaaataa
      901 tagctcgagc acacaattta accgaaacac tcaatgaccc aacagctcat gctgaaatgc
      961 aagcatttac ttcggcaaca gaatatttag ggggtaagta tttgcaaaaa gcaactttgt
     1021 atgtaacatt agaaccttgt gctatgtgcg gaggtgcttc atattgggtg caaataggaa
     1081 ctatagtata tggcgcgagt gataataaac ggggaatatc tgcgttgagg tcacatattt
     1141 tgcatcctaa aactcaaatt ataggtggta ttcttcaaac cgaatgcgaa acattgctac
     1201 agaatttttt taaacaaaaa cgataaaaat aattttttat gaaacatgga aacactatac
     1261 tacacattgt atttggattt gcaattgtag ttttatttaa tctcgttact ataaataaaa
     1321 aaacaactcc cggtaaagaa aaaactgaac ctgttcatat agcagttcag gattcaagtg
     1381 gtaatatcgt aattaaacca tttcctgttg cctatattat tgttgattca cttatgcgca
     1441 attatttata ttacaaaaaa attgaagggc aatatcaagc aatggttaaa caagaagatg
     1501 ctaaattgca acaacaaggt caagcttttg aacaagaagt gtatcaattg caatcgcaag
     1561 ttcaaaaagg attaataaca acacgcgatg ctcaaaccaa agaacaagaa ttacagctaa
     1621 aacaacaaaa gttaatggag tggcagcaat caaaacaaca agaattggca aataaagaac
     1681 aattactcat gaaagaattg cttgatagtg tacaagttgc cattaaattt cataatgctg
     1741 ataataaata tgaaatagta ttgaataatg catttaatag tagtgtattg tatgccaaag
     1801 agcatcttaa tatcaccgat actatcctta aaattatgaa tgaacgttat tcgaaatcgt
     1861 tatcaaaaaa gtaatttttt aattatctgt atttcaaacg gtttttaaaa aatatgcaca
     1921 ttgttaatta aataatagta taaaatcttg acattttttt tataaaatag tatttttata
     1981 actgaaatac tcatactttt ttaatacaac ccggatattg aagagataaa agtttatgca
     2041 cacttttaga ttatccgcac tcttcaaaaa actttaatta atagtattct aaatatcgcc
     2101 tatatccggg ttgttttttt agcataattc aaaaaaaatc aaaaatattt acatgtaagt
     2161 taaagaatag gaattctttt tatcattaca aataaaattt tatatatcta ttaggagaaa
     2221 tttttcattc acaattgttt tgtggcatat attagtttga taaagtaaaa atattattgt
     2281 ctttagatat actaaaatga tagaaaaata tgttattttt gttttgtaaa tcagatgtat
     2341 aaaatatgag aatatcaata ctcagttttg ttgtatattt ttttcttagt tattgctata
     2401 gtcaaactac agcaaatttt tcgcttcgcg acgattctca atatataatg tgttatacag
     2461 aatattctaa tggaatagca gtaaataata cagaagttga atttataaac acatcgtcat
     2521 gtacaggtaa ttgtagatat gtttttgact ttggagataa tacccctcaa ataataaaag
     2581 ataatacatc aaacacaaca cacagatatg ttcaagatgg ggtatttgat gtgcgtttgc
     2641 aagtattaaa tactgtatca attcatgata gtataaaaaa tagaccattt acatctgtac
     2701 aatttataag tcgttctgac gattcagttc aattagctat tacatataca ggtattgaca
     2761 actcaagaaa aacggctacc ataaaaatac ctgaaggtca atatgctcgc agaacattgg
     2821 cacaaccaat tacagtgtat agtccattag ttgaaagtac taatttttta tacgaaatag
     2881 atgatccgag tacagatgat tcgaaagctc ctatacagtc atttgctcat atatttactg
     2941 ttgatacagc caattttaaa cctcatgatt taagtaagtg gacatattat tgggaaattt
     3001 ataaaacaaa tgaatatggt gagccaatga atacgccata taccaaattt catgttgatt
     3061 ctattgaata taaatataca tttcctgcag aaaatttcag ccccggttat tatgtaaaat
     3121 taaaaatagc acttgatagc agt
//
LOCUS       DAOTOW010000202         3141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24263_length_3141_cov_3.350292, whole genome shotgun sequence.
ACCESSION   DAOTOW010000202 DAOTOW010000000
VERSION     DAOTOW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24263_length_3141_cov_3.350292"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="P5243_04615"
     CDS             complement(<1..1245)
                     /locus_tag="P5243_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS18764.1"
                     /translation="MEQKNTRVIIPLIIGISIFAGFYLARFVGVSPQKYSKQTDKLAA
                     LMELIETEYVDSVSRSEIIESIIPFVLEKLDPHSAYIVPEEYNEIQDPIRGSFEGIGV
                     QFNIKNDTIVIMQIIPGGPSEKVALKAGDRIITINDSLVANKGITNDKVVKLLKGPKG
                     TKVKVGIKREGHPQLLDFVITRDKIPFNSIDAAYMIEPTVGYIKISRFAATTPEEYTE
                     HITKLRNQGMTKIIIDLRDNGGGVLGSAIFLANEFLQKNDVIVYTQGKSHAKEYYRAD
                     GKGTCKDIELAVLINEYSASASEIFAGAMQDNDRGVIVGRRSFGKGLVNRDFMFADSS
                     MVRLTIQKFYSPSGRCIQKPYTHNKSDYANELLERFAHGEMQIKDSIHFPDSLKYKTK
                     QGKTVYGGGGIMPEVFVPIDTVG"
     gene            complement(1247..1690)
                     /locus_tag="P5243_04620"
     CDS             complement(1247..1690)
                     /locus_tag="P5243_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRS18765.1"
                     /translation="MGCNCNTNGDKQKNLDSRYLRMAHIWAENSYCNRRKVGAILVKD
                     KMIISDGYNGTPSGFENICEDEEGKTKPYVLHAEANAITKVAKSTNNSAGATMYVTTS
                     PCIECAKLIIQSGIKRVVYTDDYRSNDGIELLQKAGIEVCRIEIE"
     gene            complement(1743..2993)
                     /locus_tag="P5243_04625"
     CDS             complement(1743..2993)
                     /locus_tag="P5243_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS18766.1"
                     /translation="MNTELYLAKRILPHKDTANISKPIVNIAIIAIALGLAVMIVSVS
                     VIIGFKSTISDKIFGFSSHIQIVNFDTNTSYESVPISKDSILVKSIKTIPDVVHVQTF
                     ATKPGIIKVNNAVKGVIVKGIDNSFNTSFFQNHKIDGSMVTFPDTITDRVLVSKQIAD
                     LLQLHTGESFIITFVPNESSKKPRKRRFVISGIYQTNLQEIDSRFILADIRHIQALNN
                     WENNQISGYEVFLDSHKKLQKNKQLIQDEVGYDIGPDVQRLKVQTLQEVMPQIFDWLA
                     LLDMNAWVIIILMVLVAGMNMITGLLVIILEKTMNIGILKTLGIENVRIQKIFLYIGA
                     MLIGKGMLWGNIIGISLCVIQMYFKVIPLDPATYFMSYIPIYISVPAILALNVGTLIL
                     TVGMLLLPSFIISKISPAQAIRFE"
BASE COUNT         1008 a          564 c          449 g         1120 t
ORIGIN      
        1 ccctacagta tcaataggta caaaaacttc gggcattatt ccccctccac catataccgt
       61 ttttccttgt ttggttttat attttaatga atcagggaaa tgaatactgt ctttaatttg
      121 catttcacca tgcgcaaagc gttcaagcaa ttcatttgca taatcagatt tattgtgggt
      181 atatggtttt tgaatgcatc tacctgaagg agaatagaat ttttgaatag taagacgcac
      241 catagaacta tcggcaaaca taaaatcgcg attaaccaat cctttaccga aggaacgacg
      301 accaacaata actcctctgt cattatcttg catggcacca gcaaaaattt cacttgccga
      361 agccgaatat tcatttataa gaacagcaag ttctatatct ttacatgttc ctttaccatc
      421 ggcacgataa tattctttgg catgtgattt tccttgtgta tatactatta catcattttt
      481 ttgtaaaaat tcattggcaa gaaaaattgc tgaccccaat acaccaccac cattatcacg
      541 taaatcaatt ataatttttg tcattccttg atttcttaat ttggtaatat gttcggtata
      601 ttcttcgggc gttgttgctg caaatctact tattttaata tatcccacag taggttctat
      661 catgtaagcc gcatcaatac tattaaacgg aattttatca cgtgttatta caaaatctaa
      721 taattgtggg tgtccttcgc gttttatgcc aacttttact tttgttcctt taggtccttt
      781 cagtaatttt acaactttat cattggttat tcccttgttt gctaccaacg aatcatttat
      841 tgtaataatt cggtctcctg ctttgagtgc aactttttct gaaggtccac cgggaataat
      901 ttgcataatt acgatagtat cattttttat attaaattgt actccaatac cttcaaatga
      961 gccacgaatg ggatcttgaa tttcattata ttcttcgggt actatatatg cagaatgtgg
     1021 atctaatttc tccaacacaa aaggaataat ggattcaata atttcactgc gagaaacaga
     1081 atctacatat tctgtttcaa tcaattccat aagcgctgct aatttatcgg tttgtttaga
     1141 atatttttga ggcgaaacac caacaaatct tgccaaataa aatcctgcaa atattgaaat
     1201 tccaataatc aaaggtataa ttacacgtgt atttttttgt tccatattat tctatttcaa
     1261 ttctgcatac ctcaatacct gctttttgca acaattctat accatcgttg cttctgtaat
     1321 catcagtata tactactcgc tttattcccg actgtataat taattttgca cattctatac
     1381 atggtgaagt agtaacatac atagtagctc cggcactatt atttgtagat tttgcaactt
     1441 tagttatggc atttgcttcg gcatgcaaaa catagggttt tgtttttccc tcttcatctt
     1501 cacatatatt ctcaaatccc gatggagttc cattgtatcc atcagaaatt atcattttat
     1561 cttttaccag aatagctcct actttacgac ggttacagta tgaattttcg gcccaaatat
     1621 gagccatgcg taaatatcgt gaatcaagat ttttttgttt atctccgttt gtattacaat
     1681 tgcatcccat attgtatttt ttttcaaaat acgtattatt ttatcaaaat accaaatatt
     1741 gattattcaa accgtatagc ttgcgcagga ctaatttttg aaattatgaa tgaaggcaac
     1801 aacagcatac ctactgtaag aattaacgtc ccaacattaa gagccaaaat agccggaaca
     1861 gatatataaa taggtatata tgacatgaaa tatgtagccg gatcaagcgg aataacttta
     1921 aaatacattt gaataacaca caatgaaata cctataatat tgccccatag cattcctttt
     1981 cctataagca tggctcctat ataaagaaat attttttgaa ttcttacatt ttcaatacct
     2041 aaggttttaa gaattcctat attcatagtt ttttcaagaa ttataactaa taaaccggta
     2101 atcatattca tacctgcaac caaaaccatc aatataatta ttacccaagc attcatatct
     2161 aataaagcaa gccaatcaaa tatttgcggc ataacttctt gtaaagtctg tactttgagt
     2221 ctttgcacat cgggacctat atcatagcct acctcatctt gaatcaactg tttatttttt
     2281 tgcagtttct tgtgcgaatc caaaaaaact tcgtaccctg aaatttgatt gttttcccaa
     2341 ttattcaatg cctgaatatg tcgtatatcg gcaagaataa aacgactgtc aatttcttgc
     2401 aaattggttt gatatatacc cgaaattaca aatctgcgct tacgtggttt tttacttgat
     2461 tcattaggaa caaaggtaat aataaatgac tcaccggtat gtaattgtaa taaatcggct
     2521 atttgctttg aaaccaatac cctatcagta attgtatcgg gaaaagtaac catagagcca
     2581 tctattttgt gattttgaaa aaaggaagta ttaaaactat tatctatacc ttttacaata
     2641 actcctttta ccgcattgtt tactttaata attcccggtt ttgttgcaaa tgtttgcaca
     2701 tgaacaacat caggaatagt tttaattgat tttacaagaa tactatcttt tgaaatagga
     2761 acactttcgt acgatgtatt agtatcaaaa tttacaattt gaatatgtga tgaaaaacca
     2821 aatattttat cgctaattgt tgatttaaag ccaattataa ccgaaacgga aacaatcatt
     2881 acagctaaac caagagcaat tgcaataatt gcaatattta caattggttt tgatatattt
     2941 gccgtatctt tatgcggcaa tattcgtttt gctaaataga gttctgtatt catataggca
     3001 aatatacata aagttttgtt cttgaaactt ttaaatgggg aaatgatgaa gatagtgcaa
     3061 ctaattttag gaatgttgat aacgtattcg gtttgttatt ctcaattaag agtaggagct
     3121 gaatgcaccg aagattattt g
//
LOCUS       DAOTOW010000203         3137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24299_length_3137_cov_3.703439, whole genome shotgun sequence.
ACCESSION   DAOTOW010000203 DAOTOW010000000
VERSION     DAOTOW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24299_length_3137_cov_3.703439"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="P5243_04630"
     CDS             complement(<1..2272)
                     /locus_tag="P5243_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS18767.1"
                     /translation="MNISELSLKRPILATVMNLLLLIFGIVGFIQLGVREYPAIDPPI
                     VTVTTNYTGANAEVIESQITEPLEKAVNGIPGVRTISSTSSVGRSRISVEFNINIDLE
                     TATNDVRDKISQAVRSLPADIDAAPIVSKADANSEFIILVAIQSSTKSLMELSDYAEN
                     VLQDKFQTIPEVSGVNIHGQKRPAMRIWLQPDKMKAYNIAYNDISAIMQKENIEAPTG
                     KIDGVNTELTIKAAGRFTTEDDFRNMILKNTPEGIVTLGDVAKVELGPERYEQSWKLN
                     GTYGVGLSIIPQPGANYISIANEFYKRLEQIKASEKGDIEFTILIDTTKNVRRSLTEV
                     KETLIIAILLVILIIFLFFRDWLIALRPLIDIPVSLIATFFVLYLMGYSINILSLLGI
                     VLATGLVVDDGIVVTENIFRKFEEGLPIQRAALEGSKEIFFAVISTSITLAIVFLPIF
                     FMQGFVGSLFREFGIVVAVAVLISSFVSLTITPVLNVLLNSKKRNHSTFYKKTEPFFE
                     ALEQGYKKMIIGFVKKRKFAWAIVGFCIALILILGSYIQSELAPIEDKSQIRLQISAP
                     EGVSYTYMINEGEKLSDFLIDSIPERDFSFLAIPGFSSTGVNSGMGRIGLVPPNERNK
                     TQSEIAKELQRKFSQFNTIKIIPVEEQTIAVGLGSKGSMPIQFVLQNFDFEKLKQGVT
                     RVIEEARNNKTFQNIDVNLKFNKPEVTITIDRLKARELGLTISDIADVMQSAFSGRRI
                     AYFTMDGKQYQVISQVE"
     gene            complement(2272..>3137)
                     /locus_tag="P5243_04635"
     CDS             complement(2272..>3137)
                     /locus_tag="P5243_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS18768.1"
                     /translation="ANEMVDLHPEVSGRITYLNIPDGAKVTEGTILARINDADLQAQL
                     QQTKAQLSLAQAQEQRLQQLLTARGVSQAEYDAAYNEVLKIKAQLQVIQANIEKTIIR
                     APFSGTLGLRLVSPGAFVTPQTKLSTLMQTNPVKIDFQVPELYASSVTVGSHITCKTQ
                     DNKTYTATVQAIEPYIQTNTRNILVRAIITDAVLLPGTFVTIPLQQTKLAIVVPTNAI
                     IPDANKNQLVLVQNGKAQFKTVETGIRTPEYVEIVKGISIGDTILVSGMLYVRPQERV
                     IIKEVTHISMP"
BASE COUNT          903 a          541 c          552 g         1141 t
ORIGIN      
        1 cttctacttg actaattact tgatattgtt taccatccat agtaaaataa gctattcgtc
       61 ggccgctaaa ggcactttgc attacatcgg ctatatcact tatagtaagt cccaattcac
      121 gagctttgag cctatctatt gtaattgtga cttcaggttt attaaattta aggtttacat
      181 ctatgttttg aaacgtttta ttgttacgag cttcttctat tacacgagtt acgccttgtt
      241 taagtttttc aaaatcaaaa ttttgtaata caaattgtat aggcatagag cctttagaac
      301 caagtccaac ggcaatagtt tgttcttcaa ccggaattat ttttatagta ttgaattgtg
      361 aaaattttcg ttgtaattct tttgctatct ctgattgtgt tttgttgcgt tcgttgggtg
      421 gaactaatcc aattctaccc atgccgctgt ttacgccggt agaactaaaa ccgggaatag
      481 ccaaaaatga aaaatcacgt tcgggaatag aatctattaa aaaatcagag agtttttctc
      541 cttcgtttat catataagta tagcttactc cttccggtgc cgatatttgt aaacgtattt
      601 gacttttatc ttcaatagga gcaagttcac tttgaatata actgccaaga atgagaataa
      661 gtgctataca aaatcctact atagcccatg caaattttcg ttttttcaca aagccaatta
      721 tcattttttt atatccttgt tctaaagctt caaaaaatgg ttctgttttt ttgtaaaatg
      781 tactgtgatt ccgttttttt gaattgagca atacgttgag cacgggtgtt attgtaagcg
      841 aaacaaatga ggagattaaa acagcaacgg ctactactat gccaaattca cgaaataagc
      901 tacctacaaa accttgcata aaaaatattg gtaaaaaaac aattgcaaga gttatagatg
      961 tagaaattac agcaaaaaat atttctttac tgccttctaa agctgcacgt tgtatgggga
     1021 gtccttcttc aaattttcta aaaatatttt cggtaactac tataccgtca tcaactacca
     1081 aacctgttgc caatactatt cccaatagag ataaaatatt tatagaatat cccataagat
     1141 acaatacaaa aaatgttgct atcagcgata ccggaatatc tataagtgga cgaagagcta
     1201 taagccaatc gcggaaaaat aagaaaataa taagaattac taataatata gcaattatga
     1261 gtgtttcttt tacttcggtg agagaacgtc tcacattttt ggttgtatct atgagaatag
     1321 taaattctat atctcctttt tctgaagctt ttatttgttc taatcgttta taaaattcat
     1381 tggcaatact aatataattt gctccgggtt gaggtataat tgataagcct acaccatatg
     1441 taccattaag tttccaactt tgttcatatc gttcgggtcc taattctact tttgcaacat
     1501 caccaagggt aactattcct tcgggggtgt ttttaagaat catatttcta aagtcatctt
     1561 ctgttgtaaa tcgtccggca gcttttatgg taagttcggt atttacacca tcaatttttc
     1621 cggtaggagc ttctatattc tctttttgca taatagccga aatatcgttg taagctatgt
     1681 tatatgcttt cattttgtcg ggttgtaacc aaatacgcat tgcaggacgt ttttgtccgt
     1741 gaatattaac tccgcttact tcgggtatag tttgaaattt atcttgtagt acattttcag
     1801 catagtcact taattccatt aaacttttgg tgctgctttg aatagcaacc aaaattataa
     1861 attcactgtt tgcgtcggct tttgaaacaa tcggagcagc atcaatatcg gcaggaagac
     1921 ttcttacggc ttgactaatt ttgtcacgga catcatttgt tgctgtttct aaatcaatgt
     1981 ttatattaaa ttctaccgaa attctacttc ttccgactga actggttgat gaaatggttc
     2041 gaactcccgg tattccgttt acagcttttt ccaaaggttc ggtaatttgt gattctatga
     2101 cttcggcgtt agcgcctgtg tagtttgtag ttactgttac tataggtggg tctatagcag
     2161 gatattctcg tacccctagt tgtatgaacc ctactatgcc aaaaatgagt aacaataggt
     2221 tcattactgt tgctaatatc ggtcgtttga gtgaaagttc cgaaatattc attatggcat
     2281 cgaaatatgt gtaacttctt taattataac acgttcttgt gggcgaacat aaagcatgcc
     2341 tgatacaagt attgtgtcgc caatagaaat tccttttaca atttcaacat attcaggtgt
     2401 acgtatacct gtttctacgg ttttaaattg tgctttgcca ttttgtacca atactaattg
     2461 atttttgttg gcatcgggta ttatggcatt ggtaggaact acaattgcta gtttggtttg
     2521 ttgtaatgga atagttacaa atgtaccggg taataatact gcatctgtaa ttatagcacg
     2581 aaccaatata ttacgagtat ttgtttgaat atatggctct attgcttgca ctgttgctgt
     2641 atatgtttta ttatcttgtg ttttgcacgt aatatgactt ccaacggtta ccgatgaagc
     2701 gtataattca ggtacttgaa aatcaatctt tacaggattt gtttgcataa gtgtagatag
     2761 ctttgtttgt ggtgtaacaa atgctcccgg acttaccaat cgtaaaccta aagtgcccga
     2821 aaaaggtgca cgtattatag ttttttcaat atttgcttgt attacttgta attgagcttt
     2881 tattttgaga acttcgttat atgcagcatc atattctgct tggctcactc ctcgcgctgt
     2941 aagtaattgt tgtaaacgtt gctcctgagc ttgagccaat gataattggg ctttggtttg
     3001 ttgcaattga gcttgtaaat cggcatcatt tattcgtgca agtatggtac cttctgttac
     3061 ttttgctcca tcgggaatat ttaagtatgt tattcttcca cttacttcgg ggtgtaagtc
     3121 aaccatttcg tttgctt
//
LOCUS       DAOTOW010000204         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24329_length_3135_cov_3.469156, whole genome shotgun sequence.
ACCESSION   DAOTOW010000204 DAOTOW010000000
VERSION     DAOTOW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24329_length_3135_cov_3.469156"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="P5243_04640"
     CDS             <1..708
                     /locus_tag="P5243_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRS18769.1"
                     /translation="MYIIRQYIEGISLKQLIYDKELSHVYSHTIIYQIFIQLCDILND
                     IHTQGVIHRDIKPSNIIIQFDSKNSYVTSVHLVDIEMALFNGNEVIPHEKPPFALIYS
                     SPEQVLRKANLTNHTSDIFSLGISLYESLVRKTPFTHTNPELLLHMQLSIIVPKHHVL
                     PHSIYNIITKACSIPQVTKPLFTYKPQEQETLLIQSQQNRYSSVLELQKDLDQYIRQK
                     GFENSKQSVLKFLKFFK"
     gene            760..2193
                     /gene="ahcY"
                     /locus_tag="P5243_04645"
     CDS             760..2193
                     /gene="ahcY"
                     /locus_tag="P5243_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRS18770.1"
                     /translation="MDFKIANIQLAEWGRKELDLAEIEMPGLMALREEYKGKKPLAGA
                     KIMGSLHMTVQTAILIETLVELGADVRWVSCNIFSTQDHAAAAVVVGKTGTIENPKGT
                     PVYAWKGETLEEYWWCTEQALNWPDGSGPNLILDDGGDATMLVIKGAQFEAEGKVPDF
                     NPNTDSEEWGVFLALCKKVMAQDAKHWTKKLQNIRGVSEETTTGVHRLYQMAEQGLLP
                     FPAINVNDSVTKSKFDNLYGCRHSLTDGIMRASDVMLAGKVAVVCGYGDVGKGCAQSL
                     KGQGARVIITEIDPICALQAAMEGYQVLTVEDTLEYADIFVTTTGNRDIITADHMKRM
                     KNMAIVGNIGHFDNEIQMAELAKIPGIVKTNIKPQYDKWTFPDGHSILILAEGRLLNL
                     GCATGHPSFVMSASFTNQVLAQIELHTQNAKYENKVYTLPKILDEKVARLHLDKLGVK
                     LTKLTTTQANYLGVAIDGPYKPDHYRY"
     gene            2279..>3135
                     /gene="prfA"
                     /locus_tag="P5243_04650"
     CDS             2279..>3135
                     /gene="prfA"
                     /locus_tag="P5243_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRS18771.1"
                     /translation="MAENSLLEKLEVLKLKFEEVEQQITDPAIIADQKRFIQLNKEYK
                     ELQGLVEAYKTYKNTIDNIASAKQMLEEEDDAEMKEMAKAELDELQSSLPVLEEEIKM
                     LLIPKDPQDDKNAILEIRAGTGGDEASLFAGELYRMYAKYCEKKGWRISMTSCSEGTQ
                     GGYKEIICSVSGEGVYGILKYESGVHRVQRVPQTETQGRVHTSAATVAVLPEADEFDV
                     ELNMNDVRKDTYCSSGPGGQSVNTTYSAIRLTHIPTGIVVTCQDEKSQLKNLDKAIVE
                     LRTRLYNME"
BASE COUNT         1180 a          503 c          571 g          881 t
ORIGIN      
        1 atgtatatta taagacaata tatagaagga attagtctga aacaacttat atatgacaaa
       61 gaattatcac atgtgtattc acacacaatt atatatcaaa tttttattca attatgtgat
      121 atactcaatg atatacacac acagggagta atacacagag atataaaacc gtcaaatatt
      181 ataattcaat ttgattcaaa aaattcatat gtaactagtg tacatttagt tgatattgaa
      241 atggctttat tcaatggaaa tgaagtaatt ccacatgaaa agcctccgtt tgcacttatt
      301 tattcatcgc cagaacaagt gctacgtaaa gcaaatctta caaatcacac atcagatata
      361 ttcagtttgg gaatttcttt atatgaatcc ctagttcgta aaactccatt tactcacact
      421 aatcctgaac tattacttca tatgcaattg agcataattg taccaaaaca tcatgtattg
      481 ccccattcta tatacaatat tattacaaaa gcatgcagta taccacaggt aactaaacca
      541 ttgtttacat ataaaccaca agaacaagaa accttactta tacaatcaca acaaaaccga
      601 tattcttcag tacttgaatt acaaaaagat ttagatcaat atatacgtca aaaaggattt
      661 gaaaattcaa aacaaagtgt actaaaattt ttaaagttct ttaaataaaa ccaaaattaa
      721 ataatacttt tgtaacagaa taataaaaaa ataagtacta tggattttaa aattgcaaat
      781 attcaattgg ctgaatgggg acgaaaagaa cttgatttag ctgaaattga aatgcccggc
      841 cttatggctt tacgtgaaga atataaaggc aaaaaaccat tggcgggagc taaaattatg
      901 gggtcacttc atatgactgt gcaaacagct atacttatag aaacattggt tgaattagga
      961 gccgatgttc gttgggtatc atgtaatata ttttctacac aagaccacgc tgctgctgcc
     1021 gtagtagtag gcaaaaccgg cacaattgaa aatcctaaag gcaccccggt atatgcatgg
     1081 aaaggagaaa cactcgagga atattggtgg tgtaccgagc aagctctaaa ttggcctgac
     1141 ggtagtggcc cgaaccttat attagatgat ggtggagatg ctacaatgtt agttataaaa
     1201 ggagctcaat ttgaagctga gggcaaagta cctgatttta atccaaacac tgattctgaa
     1261 gaatggggag tttttctagc attgtgtaaa aaagtaatgg cacaagatgc taaacattgg
     1321 actaaaaaac tacaaaatat tcgaggtgtt tccgaagaaa ctactaccgg agttcatcgt
     1381 ttatatcaaa tggcagaaca aggattattg ccttttcctg caataaatgt aaatgattcg
     1441 gtaaccaaat caaaatttga taatttatat ggttgtcgcc attcattaac cgatggtatt
     1501 atgcgtgcat ctgatgtaat gctggcagga aaagttgctg tggtatgcgg ttatggtgat
     1561 gtaggaaaag gctgtgctca atcactcaaa ggtcaaggag cacgagtaat aattaccgaa
     1621 attgacccaa tttgtgcttt gcaagctgca atggaaggct atcaagtact cactgtagaa
     1681 gacacattag aatatgctga tatttttgtt acaaccacag gaaacagaga tataataact
     1741 gccgaccata tgaaacgaat gaaaaatatg gctatagttg gcaatattgg tcattttgac
     1801 aatgagattc aaatggcaga attagctaaa attccgggga ttgtaaaaac aaacataaaa
     1861 cctcaatacg acaaatggac attccccgat ggtcacagca ttttaatttt agccgaaggt
     1921 cgtttattga atcttggttg tgcaaccgga catccaagtt ttgtaatgtc tgcatcgttt
     1981 acaaatcaag tattggcaca aatagaatta catactcaga atgcaaaata tgaaaataaa
     2041 gtatatacat taccaaaaat acttgatgaa aaagttgctc gattacatct tgataaattg
     2101 ggagtaaaac tgacaaaatt aacaacaaca caagctaatt atttaggtgt tgctatagac
     2161 ggaccataca aacccgacca ttacagatat taataaaaaa ctcatactcg aaaaaaagct
     2221 gttgatttgt tcaacagctt tttttttgta catttgccgc gaaataaatt tatataaaat
     2281 ggcagaaaat tctttattag aaaagcttga agttcttaaa cttaagttcg aagaagttga
     2341 acaacagata acagatccgg caattatagc cgatcaaaaa cgttttattc aactcaataa
     2401 agaatacaaa gagttgcaag gattggtaga agcatacaaa acatacaaaa ataccattga
     2461 caatatagca tcggcaaaac aaatgcttga agaagaagat gatgccgaaa tgaaagaaat
     2521 ggcgaaagcc gaacttgatg aattgcaaag ttctcttcct gttttagaag aagaaattaa
     2581 aatgttgctt ataccaaaag atccgcaaga tgataaaaat gcaattttag aaattcgtgc
     2641 cggaaccgga ggcgatgaag cttctctatt tgccggagaa ttgtatagaa tgtatgcaaa
     2701 atactgcgag aaaaaaggat ggagaatttc aatgaccagt tgtagtgaag gtacccaagg
     2761 tggatacaaa gaaataatat gcagtgttag cggcgaaggt gtatatggta ttttaaagta
     2821 tgaatcgggt gttcatcgtg tgcaacgagt tccacaaaca gaaacacaag gacgtgtaca
     2881 tactagtgca gcaacagttg ctgtattacc cgaagccgat gaatttgacg tagaattaaa
     2941 catgaacgat gtgagaaaag acacatattg ttcatcggga cctggtggac aatcagtaaa
     3001 tactacctat tctgctattc gtttaacaca tattcctaca ggtatcgttg ttacatgtca
     3061 agacgaaaag tcacaattaa agaatttaga taaagctatt gttgaattac gcactcgatt
     3121 atacaacatg gagta
//
LOCUS       DAOTOW010000205         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24332_length_3135_cov_2.971104, whole genome shotgun sequence.
ACCESSION   DAOTOW010000205 DAOTOW010000000
VERSION     DAOTOW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24332_length_3135_cov_2.971104"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="P5243_04655"
     CDS             complement(<1..675)
                     /locus_tag="P5243_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18772.1"
                     /translation="MGKQAINQQAHATIQQSTHSDSLGIISLQPSQSQGIQFPYAYSQ
                     QQSVTDTLQAQEQIVQLPKETINNQSIRFPYIVSNSAPDLDFSVSMFEYGDSVDFSHK
                     LEKTKQKEFTQEFKEKVFVINYKQINETTNNWMFWLILGVLVLFGWSRLFYKKQLELL
                     MSAILHYNFAVKGIRNASENSQRFSNMLQFIFSVNIALFTYQVYNYFVPLTLTGFKAL
                     GVVLIIT"
     gene            1034..2530
                     /locus_tag="P5243_04660"
     CDS             1034..2530
                     /locus_tag="P5243_04660"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRS18773.1"
                     /translation="MNDKVFVFDTTLRDGEQAPGNQLNMVEKIEVAKALELLGVDIIE
                     AGFPISSPGDFDSVVQISKAVTNPIICGLTRAVQKDIEVAAEALKYAKRPRIHTGIGV
                     SPYHIVSKLNSTPEEIIRRGVDAVKYAKKFVEDVEFYCEDAGRSDNEYLARVVEAVIA
                     AGATVVNIPDTTGYCLPHEYGAKIKYLIDNVPNIHKAILSTHCHNDLGMATANTMSGV
                     LNGARQVEVTLNGVGERAGNTAMEEVVMAIKSHKLGVYTDIVTPRILQTSRLVQRLMK
                     SPVQANKAIVGKNAFAHSSGIHQDGVLKNRENYEIIDPKDVGVDESSIILTARSGRAA
                     LRHRLELLGFKIDGADLDKTYQDFLHLADVKKEVKDNDLLVLVGKEQRDANRAIKLDS
                     LQVVCGKSMIPSATVSLIIQGETKTETAMGDGPVDASYNAVKKLIDKEIKLLEFLIQG
                     FNNGSDDFGQVNVQIECNKTIYYGFGADTDIVTASVEAFVDAISKSME"
     gene            2627..>3135
                     /locus_tag="P5243_04665"
     CDS             2627..>3135
                     /locus_tag="P5243_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRS18774.1"
                     /translation="MGKTLFDKIWDAHVVTEIQDGPSILYIDRQFIHEVTSPQAFTGI
                     EARGRKVFRPAQITATADHNIPTMDQHLPIKEYLSAFQVEKLKENSARHGITYYGLGD
                     EYNGIVHVVGPELGYTQPGMTIVCGDSHTSTHGAFGTVAFGVGTSEVEMVFASQCIMQ
                     PKPKRMRIT"
BASE COUNT         1065 a          515 c          580 g          975 t
ORIGIN      
        1 tgttattatt aatacgactc caagagcttt gaatccggta agagtaagtg gcacaaaata
       61 attatagacc tgatatgtaa acaaagcaat atttaccgaa aatataaatt gcaacatatt
      121 tgaaaaacgc tgtgaatttt cacttgcatt acgaatacct tttactgcaa aattataatg
      181 taatatagcc gacatcaaca attccaattg ttttttataa aacaatcgcg accatccaaa
      241 caataccagc actcctaaaa taagccaaaa catccaattg ttggtagttt cgtttatttg
      301 cttataattt attacaaaaa ccttttcttt aaattcttgc gtaaattctt tctgttttgt
      361 tttttccaat ttatgtgaaa aatctaccga atcaccatat tcaaacattg acactgaaaa
      421 atccaaatct ggagctgaat tggatactat atatggaaac cgaatcgatt gattattgat
      481 tgtttctttg ggtaattgaa caatttgttc ttgtgcctgt aaagtatcag ttactgattg
      541 ctgttgacta taagcataag ggaattgaat accttgagat tgtgatggtt gcaatgaaat
      601 aatacccaaa gaatcactat gtgtgctttg ttgtatagtt gcatgagctt gttgatttat
      661 cgcttgctta cccaatgtaa tcgttttttt gcaaaattac aaaacatgct aattgtcata
      721 tactatattg tattattttt tattaaatag ttatgaacgt agtatttaag aattacagat
      781 ttataaaaaa tctgtaattt ttctttgttt tttttgacta ttatataaaa taaaatatat
      841 ttgcagtaaa taaataaaca gatgaaacaa ttaattacca tattactcaa cctgctgctc
      901 attagctaat gctagggggt tgttggtata tacagtaata tgagtaacaa taattacaat
      961 ataaaaagcc ccctttgctt ctaagctttg ggggttttta ttttttttta ataaatatgt
     1021 aaacaaaata aatatgaatg ataaagtttt tgtgttcgac accaccttac gtgatggcga
     1081 acaagcaccg ggaaatcaat taaacatggt tgaaaaaatt gaagttgcaa aagctttaga
     1141 acttcttggt gttgacatta ttgaagccgg atttcctatt tcaagtcccg gtgattttga
     1201 ttcggtagta caaatttcaa aagcagttac caatcccatt atctgcggat tgactcgtgc
     1261 tgttcaaaaa gatattgaag tagctgccga agctcttaaa tacgcaaaac gtccgagaat
     1321 tcataccggt attggtgtat caccgtatca tatagtgtca aaattaaatt ctacccccga
     1381 agaaattatt cgtcgtggtg ttgacgctgt aaaatatgcc aaaaaatttg tagaagatgt
     1441 agaattttac tgtgaagatg ccggacgcag cgacaatgaa tatcttgcac gtgtagtaga
     1501 agctgtaatt gctgcaggtg ctaccgtagt taacattccc gatactaccg gctattgctt
     1561 accgcatgaa tatggtgcaa aaatcaaata tttaatagat aatgtgccaa atattcataa
     1621 agctatactt tctacccatt gccacaacga tttgggaatg gcaactgcca ataccatgtc
     1681 tggagtactt aatggagcac gccaagttga agttaccctt aacggtgtag gtgaacgcgc
     1741 agggaataca gctatggaag aagttgtaat ggcaataaaa agtcacaaat taggcgtata
     1801 taccgatatt gttactcctc gcatattaca aaccagccgt ttggtacaac gattaatgaa
     1861 gagtccggta caagcaaaca aagctattgt tggtaaaaat gcatttgctc attcttcggg
     1921 aatacatcaa gacggtgtgt taaaaaatcg tgagaattac gaaattattg accctaaaga
     1981 tgttggtgtt gacgaatcgt ctattatcct tacagcacgt agtggccgtg ctgcattacg
     2041 tcaccgatta gaattgttgg gattcaaaat tgacggtgcc gatttagaca aaacatatca
     2101 agactttttg catttggctg atgtaaaaaa agaagtaaaa gacaacgatt tactagtatt
     2161 ggtaggcaaa gaacaacgcg atgccaaccg tgccataaaa ttggattcat tgcaagtagt
     2221 atgtggtaaa tctatgattc catcggctac ggtatcgtta atcattcaag gcgaaaccaa
     2281 aacagaaaca gctatgggcg acggtccggt agatgcttct tacaatgctg tgaaaaaatt
     2341 gattgataaa gaaataaaac tgttagaatt tttaattcaa ggattcaaca atggtagtga
     2401 tgattttgga caagtaaacg tacaaataga atgtaacaaa actatctatt acggttttgg
     2461 tgccgatacc gatattgtaa ctgcttcggt tgaagcgttt gttgatgcta taagtaaatc
     2521 aatggagtag aataattttt aattatgaat ttttaatttt taatttttgg ttacatttgc
     2581 tccataaaaa taaaacataa tactgaacaa taaattaaaa caaacaatgg gaaaaacctt
     2641 attcgacaag atttgggatg cacacgtggt gacagagata caagatggtc cgagtatatt
     2701 atacatagac cgtcagttta ttcatgaggt tacaagcccc caagcattta caggaattga
     2761 agcacgcgga cgtaaagtgt ttcgtcctgc acaaattaca gctactgccg accacaacat
     2821 tccaactatg gatcaacatt taccgattaa agaatattta tcggcatttc aagttgaaaa
     2881 acttaaagaa aacagtgctc gtcatggcat tacatattat ggtttaggcg atgaatacaa
     2941 tggtatagtt cacgtggtag gacctgaatt aggttatact caaccgggta tgaccatagt
     3001 atgtggcgac agccatactt caacacacgg cgcatttggt acggtggcat ttggcgttgg
     3061 taccagcgag gtagaaatgg tgtttgcaag tcaatgcata atgcaaccta aaccaaaacg
     3121 tatgcgtata accat
//
LOCUS       DAOTOW010000206         3133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24350_length_3133_cov_3.089019, whole genome shotgun sequence.
ACCESSION   DAOTOW010000206 DAOTOW010000000
VERSION     DAOTOW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24350_length_3133_cov_3.089019"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="P5243_04670"
     CDS             <1..696
                     /locus_tag="P5243_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRS18775.1"
                     /translation="LTNDASRSPELLVKGYRNAGITEITEHKIISSGMLAREYIQNKV
                     AHGTVAYLGTEASAHYIEKEGLKTISIKDVDIENCNDITALVFLDDEGYDWNVDLNKT
                     LNLLRKRAIPVIVANTDGTYPVAKKDVAISVGAVADMIEKLARKTFIRFGKPDSQIFN
                     YAFEYAVSDDSATKNDILMVGDSLITDIIGGNKFGVDTCLVLTGNILPDNYELMIQSL
                     GIIPDYICESVAT"
     gene            complement(702..2177)
                     /gene="crtI"
                     /locus_tag="P5243_04675"
     CDS             complement(702..2177)
                     /gene="crtI"
                     /locus_tag="P5243_04675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRS18776.1"
                     /translation="MKQTVLIVGAGLGGLATALRLSKQGYSVQILEKNDTAGGRLNQI
                     QKDGFTFDTGPSFFSMSYEFEAFAKECNISLPFTFVELDPLYSVHISGSNKTWHIYKD
                     TKLFAEQFTDIEVDFEKKFEEYIAECSSIFHDTVDIVIKRNFSSKFAYFIELAKVNPF
                     HIPKLFKTFWQQVCQYFSSQEARQILSLVAFFLGKTPFDTSSVYTLLSYTEFKHDGYY
                     NVEGGMYQIVTGILHELEKQQVPILYNTEIYGVQNTKNTITEVIDTQGNSYKADIFVI
                     NADAAVFRSRILKHKSYNESKLLKKDWTMGYLTFYLGISTKLPQVHHHNYYLGSNYEE
                     YSKHVLQDPSIIEKPYYYVNVVSKYNTNCAPEGCESLFFVCPVPNLIHKSSWHDAEQI
                     VNSIIQDFSQRIELNIEPLIISKTIYTPADWEKKCNLFKGSGLGLSHSIKQIGGLRPR
                     NFDEKFKNMFYVGASTVPGAGLPMAIISSKLAAERVINYSK"
     gene            complement(2174..2833)
                     /locus_tag="P5243_04680"
     CDS             complement(2174..2833)
                     /locus_tag="P5243_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRS18777.1"
                     /translation="MPIIHIPYIKSNNRILQLMLISFFTIGTIGFLYPATKQILITLI
                     PYVLLGNFILVALFDSSQNTKKQLLVYAIIFLLGLGIEIIGVNTSIIFGTYWYGKALG
                     IQVVNTPLLIGLNWLFLVYSTRLLFQSFTIHPYLKIIGASGSMLLYDFFLEQSAHKMD
                     MWYWHNNTIPIQNYIAWFIISVCMHTIVNYAQITISNSIAKTIVYIQFVFFIILFIGL
                     S"
     gene            complement(2838..>3133)
                     /locus_tag="P5243_04685"
     CDS             complement(2838..>3133)
                     /locus_tag="P5243_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRS18778.1"
                     /translation="QRMYFPNLLHHEFTETVKQEIITDIANDFNEAYKGITLLPRNSQ
                     LAVFTAFLYYKTLLNTLQQTPAQQIAHKRIRVNNIKKILLICKAYCICKLRLL"
BASE COUNT         1047 a          446 c          517 g         1123 t
ORIGIN      
        1 cttacaaatg atgcttctcg aagtcccgaa ttacttgtaa aagggtatcg taatgcaggg
       61 attacagaaa ttactgaaca taagattata tcgtcgggaa tgcttgctcg tgaatatata
      121 caaaataaag ttgctcatgg aaccgtagca tacttgggaa ccgaagcttc tgctcattat
      181 attgaaaaag aaggacttaa aacaatttcg ataaaagatg ttgatattga aaattgtaat
      241 gatattactg ctcttgtatt cttagacgat gaaggctatg attggaatgt agacttgaac
      301 aaaacattga atttgcttcg taaacgtgca ataccggtaa ttgtagcaaa tacagatgga
      361 acatatcctg tagcaaaaaa agatgttgct atttcggtag gagctgttgc cgatatgatt
      421 gaaaaacttg cccgtaaaac atttattaga ttcggaaagc ctgattctca aatttttaac
      481 tatgcatttg aatatgctgt ttctgatgat tctgctacca aaaatgatat acttatggtg
      541 ggagactcac ttattaccga tattataggt ggtaataaat ttggtgttga tacgtgtttg
      601 gttctcaccg gaaatatttt gcctgataat tatgaactaa tgattcaatc gttaggtatt
      661 atacccgact atatttgtga atcggttgca acctaagaat ttcatttact atagtttata
      721 actctttccg ctgctagttt cgaacttata atcgccatag gtaatcctgc accgggcacg
      781 gtagatgctc ctacataaaa catgtttttg aatttttcat caaaattgcg aggacgtaat
      841 cctccaattt gtttaatact atgagataat cctaaaccac tacctttaaa taaattacat
      901 tttttttccc aatcagcagg ggtatatata gtttttgata ttataagtgg ttctatattt
      961 aattctattc tttgagaaaa atcttgtatt atgctgttaa ctatttgttc agcatcgtgc
     1021 catgacgatt tatgaattaa gtttggtaca gggcatacaa aaaataaact ttcgcatcct
     1081 tcaggagcac aattggtgtt gtattttgaa actacattaa cataataata gggtttttca
     1141 atgattgaag gatcttgtaa tacatgtttt gaatattctt cataattact tcctaaatag
     1201 taattatgat gatgaacttg aggtaatttt gttgaaattc ctaaataaaa ggtgagatac
     1261 cccatagtcc aatctttttt taaaagtttt gattcgttat aggatttgtg tttaagaatt
     1321 ctgcttctaa aaacagcggc atcagcattt ataacaaata tatcggcttt gtagctattc
     1381 ccttgagtat ctattacttc tgtaattgta ttttttgtgt tttgtacacc ataaatttcg
     1441 gtattgtaaa gtattggtac ctgttgcttt tctaactcat gcaaaatacc agtaactatt
     1501 tgatacattc ctccttctac attgtaataa ccatcgtgtt tgaattcagt atatgaaagt
     1561 agggtataaa ctgatgatgt atcaaaaggt gttttgccaa gaaaaaatgc tactaaagaa
     1621 aggatttgac gagcttcttg tgatgaaaaa tattgacata cttgctgcca aaatgttttg
     1681 aataacttag gaatatggaa tggatttact tttgcaagtt caataaaata tgcaaatttt
     1741 gaactgaaat tacgttttat tacaatatca acagtatcat gaaatataga agagcattct
     1801 gcaatatatt cttcaaattt tttttcaaaa tcaacttcaa tatcagtaaa ttgttctgca
     1861 aatagttttg tgtctttata aatatgccat gttttgttac tgccggaaat atgaacagaa
     1921 tagagtgggt ctaattcaac aaaagtaaat ggtaatgaaa tattgcattc ttttgcaaat
     1981 gcttcaaatt catatgacat actaaagaaa ctgggacctg tatcaaatgt aaatccatct
     2041 ttttgtattt gattgagtcg gccacctgca gtatcatttt tttcaagaat ttgcacagaa
     2101 tacccttgtt ttgataatcg taaagcggta gctaatccgc ctaatccggc acctactatt
     2161 aatactgttt gtttcatgat aacccaataa ataaaattat aaagaataca aattgaatat
     2221 atactattgt ttttgctata ctatttgata ttgtaatttg agcatagttt actattgtat
     2281 gcatacatac agaaatgata aaccatgcaa tataattttg tattggaata gtattgttgt
     2341 gccaatacca catatccatt ttatgagcac tttgttccaa gaaaaaatca tataaaagca
     2401 tactccccga tgctccaatt attttaagat atgggtgaat tgtaaatgat tgaaataata
     2461 aacgtgtgct gtatactaaa aataaccaat tcaatccaat taaaaggggg gtgtttacta
     2521 cttgtattcc taatgctttg ccataccaat atgtgccaaa aataatagaa gtatttactc
     2581 caataatttc aatacccaat cccaataaaa atataatggc atatacaagt aattgttttt
     2641 ttgtgttttg tgatgaatca aataatgcta caagtataaa attccctaat aaaacatagg
     2701 gaatgagtgt aataagtatc tgttttgttg cgggatataa aaaacctatt gtgccaattg
     2761 taaaaaaaga aataagcatt aattgcaata ttctattgtt actttttata tagggaatat
     2821 gtattatagg catagtgtta taataatcgc aatttacaga tacaataggc tttacaaatc
     2881 aacaggattt tttttatgtt gtttactcgt attcgtttgt gtgctatttg ttgagccgga
     2941 gtttgttgta atgtattgag tagagtttta taatataaaa atgcggtaaa tacagctaat
     3001 tgagaattac gcggaagtaa tgtaatacct ttgtatgctt cattaaagtc atttgctatg
     3061 tcggttatta tttcttgttt tactgtttcg gtaaattcgt ggtgtaataa atttggaaaa
     3121 tacatacgtt gca
//
LOCUS       DAOTOW010000207         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24358_length_3132_cov_3.250244, whole genome shotgun sequence.
ACCESSION   DAOTOW010000207 DAOTOW010000000
VERSION     DAOTOW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24358_length_3132_cov_3.250244"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="P5243_04690"
     CDS             <1..262
                     /locus_tag="P5243_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRS18779.1"
                     /translation="EGSVIVQKVREGKGDFGYNAREERFENLKAAGIVDPKKVTRIAL
                     ENAASIAGMLLTTECVLAEQKKDEPAMPMGGAPGMGGMGGMM"
     gene            446..910
                     /gene="coaD"
                     /locus_tag="P5243_04695"
     CDS             446..910
                     /gene="coaD"
                     /locus_tag="P5243_04695"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRS18780.1"
                     /translation="MEQTKRIAVFPGSFDPFTIGHKAILDMALPLFDTIIVAIGVNSE
                     KKDFFPIEKRINHILSIYTNNPKIQVETYTSLTIDFCKLKNAMFILRGLRNTTDFQFE
                     KSIAQMNRQLSPNIETVFLVTPPEYGHVSSTIVRELIRYGADISKYIPGNID"
     gene            916..2379
                     /locus_tag="P5243_04700"
     CDS             916..2379
                     /locus_tag="P5243_04700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRS18781.1"
                     /translation="MKFFSFVLFTFFSFLYSFSQDTTTIFACDREYANMHIDVYKYSD
                     YFTKIEEKVGSFNVDSLGCFTFSFPNNTTQEIFMYLGIYKVFLFAEPGMTYELAMPPF
                     EEKTLAEELNPYFVPQDLSFGVKNNLDPHEINMLILSFNAIYESFVSMNFQYIYSLRD
                     KRIVDVLEQQIDSAFDAHEVDTFFNAYKNYQIYNLRFMAYQRDRMAMTRRHYLNKPFY
                     YYNPAYVNFFQNMWKNYINNNHMKDMGKDIKVAIIYGKSPTMFKEAMEKYIPFRNDTL
                     KELFLLQCLDDCFKDPDIFPRAPVIQTLDSLILMTKVPEHALIAQNIKKKRTVLAQGD
                     LAPDFVLYNQDSVAYSLSQFAGKYVYLNFCRSENYACIQDYKVMQKMNEKTKRELQIV
                     TLCYEKDFETFRNFKRKNPQYKWIFLYAGDHPEIKDYYKFKATPSYMLIDKQGKIELQ
                     NAPSPIDNFQEKFAQILIAKRKEELELKRKQRLYQTW"
     gene            2500..>3132
                     /locus_tag="P5243_04705"
     CDS             2500..>3132
                     /locus_tag="P5243_04705"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRS18782.1"
                     /translation="MSFESERIVSEGLTFDDVLLVPAYSEVLPREVDVTSKLSKNIVL
                     NSPIVSAAMDTITDSRLAIALARQGGIGIIHKNMSIEQQAREVKRVKRSENGMIYDPI
                     TILPDATAQNALDLMTEFKIGGIPVVDTNKKLLGIVTNRDLRFESNMQRSISDIMTKD
                     NLITIKQSDDVSKAGDILQTYKIEKLPVVDDNNILVGLLTYKDIFKAKDNP"
BASE COUNT         1171 a          473 c          500 g          988 t
ORIGIN      
        1 tgaaggttct gtaatagttc aaaaagttcg cgaaggaaaa ggtgattttg ggtataatgc
       61 tcgtgaagaa cgatttgaaa acttaaaagc agccggtatt gtagatccta aaaaagttac
      121 tcgtatagca ttggaaaatg cagcatcaat tgcaggaatg ctattgacaa ccgaatgtgt
      181 attggcagaa caaaagaaag atgaacctgc tatgccaatg ggtggagctc ccggcatggg
      241 aggaatgggc ggcatgatgt agtttttagc taccctatat atattcaaaa agggtcttgc
      301 aacagtagtt gtaaggcctt tttttatatc ttatagttta taacatacaa caatatgata
      361 ccattctgtg tattaaacta caaattatac attttgattc aataaaaaaa ctatttttgc
      421 aatacaacaa acttgtaaaa ctacaatgga acaaacaaaa cgcatagcag tttttccggg
      481 aagttttgat ccgtttacaa ttggacacaa agctatttta gacatggctt tacctttatt
      541 tgacacaata atagttgcaa taggggtaaa ttctgaaaaa aaagattttt ttcctattga
      601 aaaaagaata aaccatattc tttctattta tacaaacaat ccaaaaatac aagttgaaac
      661 atatacatcg ttgactattg atttttgtaa actcaaaaat gcaatgttta tattacgagg
      721 actacgaaat acaaccgatt ttcagtttga aaaatctata gcacaaatga atagacaact
      781 ttcgccaaat attgaaacag tgtttttagt tactcctccc gaatatggac atgtttcatc
      841 tacaatagtt cgagaactta ttcgttatgg agctgatatt tctaaatata ttccggggaa
      901 cattgattga tgattatgaa gttttttagt tttgtattat ttactttttt ttctttctta
      961 tactcatttt cacaagatac aacaacaata tttgcttgcg atagagaata tgcaaacatg
     1021 catattgatg tatataaata ttccgattat tttaccaaaa tagaagaaaa agtaggttca
     1081 ttcaatgtag atagtttagg ttgtttcaca ttctcatttc ccaataatac aacacaagaa
     1141 atatttatgt acttaggtat ttacaaagtg tttttgtttg ccgaaccggg aatgacctat
     1201 gaacttgcaa tgcctccatt tgaagaaaaa acacttgccg aagaattaaa tccatatttt
     1261 gttccccaag atttaagttt tggagttaaa aacaatcttg atcctcacga aatcaacatg
     1321 cttattcttt cgtttaacgc tatttatgaa tcatttgtta gtatgaactt tcaatatatt
     1381 tattcgttac gcgacaaacg tatagttgat gtattagaac aacaaataga cagcgctttt
     1441 gatgcacatg aagttgatac attttttaat gcttacaaaa attatcaaat ttataattta
     1501 cggtttatgg cgtaccaacg agatagaatg gctatgactc gtcgtcatta tctaaataaa
     1561 ccgttctatt attataatcc tgcatatgta aattttttcc aaaatatgtg gaaaaactac
     1621 ataaacaata accatatgaa agatatgggt aaagatataa aagttgcaat aatatatggc
     1681 aaaagtccaa ctatgtttaa agaagcaatg gaaaaatata taccatttag aaatgatact
     1741 ctcaaagaat tatttttact gcaatgttta gatgattgct ttaaagatcc tgatatattt
     1801 ccgcgagctc ccgttataca aactttagac tcattaatac ttatgactaa agtccccgaa
     1861 catgctctta ttgctcaaaa tattaagaaa aaaagaactg ttttagccca aggagatctt
     1921 gctccggatt ttgtattgta taaccaagac agtgtagcat attcattaag tcaatttgca
     1981 gggaaatatg tgtacttaaa tttttgtcgt tccgaaaact atgcatgtat tcaagattac
     2041 aaagtaatgc aaaaaatgaa cgaaaaaaca aaacgagaac tccaaattgt aacgctctgt
     2101 tatgagaaag attttgaaac atttagaaat tttaaacgaa aaaatcctca atacaaatgg
     2161 atatttttat atgccggcga ccatcccgaa attaaagatt attacaaatt taaagcaact
     2221 ccatcgtata tgcttataga caaacaaggc aaaatagaat tacaaaatgc accctctcca
     2281 attgataact ttcaagaaaa atttgcacaa attcttatag caaaacgcaa agaagaatta
     2341 gaattgaaac ggaaacaacg tttatatcaa acatggtaag attttttggg gttaacaatc
     2401 attaaattta tatcaatcaa cggaaagatt acaatatttt acgcgttttg aaagaaaaat
     2461 ttaactactt ttgtgcaatt ataatttaac aaaagcagta tgtcatttga atcagaaaga
     2521 atagtatcgg aagggttaac atttgatgat gtgttacttg ttcctgcgta ttcggaggta
     2581 ttgcctcgcg aagtagatgt aacttcaaaa ttatcgaaga atattgtatt aaacagtcct
     2641 attgtatctg cagctatgga taccattacc gattcgcgac tggctatagc tcttgcacga
     2701 caaggcggta taggaataat tcataaaaac atgagtatag aacaacaagc acgtgaagta
     2761 aaacgcgtaa aacgttctga aaatggtatg atttacgacc caataacaat attgcctgat
     2821 gctacagcac aaaatgcatt agatttaatg acagaattta aaattggagg tataccggta
     2881 gttgatacta ataaaaaatt attgggtatt gtaaccaatc gtgatttacg gtttgaatct
     2941 aatatgcaac gaagcatatc agatattatg acaaaagaca atctcattac aataaaacaa
     3001 agtgatgacg tatcaaaagc cggagatatt ctacaaacat acaaaattga aaaactgccg
     3061 gtagttgatg ataataatat attagttgga ttacttactt ataaagatat atttaaagca
     3121 aaagataacc ca
//
LOCUS       DAOTOW010000208         3121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24463_length_3121_cov_2.952381, whole genome shotgun sequence.
ACCESSION   DAOTOW010000208 DAOTOW010000000
VERSION     DAOTOW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24463_length_3121_cov_2.952381"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="ribA"
                     /locus_tag="P5243_04710"
     CDS             <1..826
                     /gene="ribA"
                     /locus_tag="P5243_04710"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRS18783.1"
                     /translation="AERGGVLRRAGHTEAAVDLSRLAGLTPGGVLVEIMNEDGTMARL
                     PDLMKISEKFGLKIVSIKDLIAFLIDKESLVEMGERVKLPTDFGDFELIPFKQLSTGM
                     EHVALIKGSWDIHEAVLVRVHSSCVTGDIFMSQRCDCGHQLHEAMKLVQKEGKGVVLY
                     MNQEGRGIGLFNKIKAYKLQEQGLDTVQTNIELGFKDDERDYGVGATILCLLGIRKMR
                     LLTNNPTKRAGLEGYGLEIVENIPIEVPVNEHNHFYMQTKKDKMGHTLDFCCHSKK"
     gene            852..1832
                     /locus_tag="P5243_04715"
     CDS             852..1832
                     /locus_tag="P5243_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HRS18784.1"
                     /translation="MELYPLKFEHIYKDKIWGGTRFKTLLKRVDVPSDTCGESWEISA
                     VEDNISIVANGFLQGNSLQEIIEIYMDEIVGGKVYEQFGPEFPLLIKFLDSNDVLSVQ
                     VHPDDEVAKIRHNAYGKTEMWYVVNADTDAELIIGFDKDSSPEEFIEAVQNNCIEDLL
                     HKQVVHNDEVFFLPAGRIHAIGKGLLIAEIQQTSDITYRIYDFNRTDEHGNTRELHVD
                     LAVDVIDYKAYTDYKTHYTLTQGPSSELVSCKYFTTNILHFSKPIRKNYYEFDSFVIY
                     MCLKGEAFIDFGADYTIPIQQGETVLIPAALYEIELLTSCETKIIEVYIA"
     gene            1832..2407
                     /locus_tag="P5243_04720"
     CDS             1832..2407
                     /locus_tag="P5243_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRS18785.1"
                     /translation="MIQIGNTLISLDILEKKFICDITKCKGICCVEGDSGAPLTDDET
                     KVLSESLPQIEPFLDSVHVEILKKQGVFYIDHEGEKVTTLVNNAECAFVVKKDGVLFC
                     GIELAYKQGLVSIPKPISCHLYPIRCKQYRDFEALNYDTWYICKDAVNKGNNEGVALY
                     QFLKAPLIRKYGEQWYAELEMIAHQWKRSDK"
     gene            2547..>3121
                     /gene="prs"
                     /locus_tag="P5243_04725"
     CDS             2547..>3121
                     /gene="prs"
                     /locus_tag="P5243_04725"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRS18786.1"
                     /translation="MSNNQQIKIFTGTASRYLAEKIVAHYGIELGDSSVTFFSDGEFE
                     PRFDVSVRGKDVYIVQSTFQPSDNLMELLLMVDAAKRASARQVTAVMPYYGFARQDRK
                     DKSRVSIGAKLIANLLTAAGVDRVVTMDLHAGQIQGFFDVPVDHLYASTIFVPYLRSL
                     QIPDLLIAAPDMGGTKRAKSYAKFLNADIAI"
BASE COUNT         1092 a          401 c          614 g         1014 t
ORIGIN      
        1 ggctgaacgt ggcggagttt taagacgggc tgggcatact gaagctgccg ttgatttatc
       61 aagattagcc ggtttaactc ccggaggggt attggtagag ataatgaatg aagatggtac
      121 tatggctcga ttacccgatt tgatgaaaat aagcgaaaaa tttggtttaa aaatagtttc
      181 aattaaagat ctaattgcat ttttaattga taaagaaagt cttgttgaaa tgggtgaacg
      241 agtaaagtta ccaacagatt ttggcgattt tgaattaatt ccctttaaac aattatctac
      301 cggaatggag catgttgctc taataaaagg ttcatgggat atacatgaag ctgttttggt
      361 tcgtgttcat tcatcatgtg ttaccggtga tatttttatg agtcaacgct gcgattgcgg
      421 acatcaattg catgaagcaa tgaagttagt acaaaaagaa ggcaaggggg tagtgttgta
      481 catgaatcaa gaaggtagag gtattggttt gtttaacaaa ataaaagcat ataaactaca
      541 agaacaagga ttagatactg tacagacaaa cattgaatta ggttttaaag atgatgaacg
      601 tgattatggt gttggtgcaa caatattgtg tttacttggt attcgtaaaa tgcgattgtt
      661 gactaataat cctacaaaac gtgcaggctt agaaggatat ggtttagaaa ttgttgaaaa
      721 tataccaata gaagtacccg taaatgagca taatcatttt tatatgcaaa caaaaaaaga
      781 taaaatgggg catacattag atttttgttg tcactctaaa aagtaaatat caatttataa
      841 tatatatatc aatggaatta tatcctttaa aatttgaaca tatttataaa gataaaattt
      901 ggggtggaac acgctttaaa accttattaa aacgtgttga tgttccttct gatacgtgtg
      961 gcgaaagttg ggaaatttct gcagttgaag ataatatttc tattgtagca aatggttttt
     1021 tgcaagggaa ttctctacaa gaaataatag agatatatat ggacgaaatt gttggtggta
     1081 aagtgtatga acaatttgga ccggagtttc ctctattaat taaattttta gattcaaacg
     1141 atgtattgtc agtgcaagtg catccagacg atgaagttgc aaaaatacgt cacaatgcct
     1201 atggtaaaac agaaatgtgg tatgtagtta atgcagatac tgatgcggaa ttaataatag
     1261 gtttcgataa agatagtagc cctgaagaat ttattgaagc tgtgcagaat aattgcattg
     1321 aagatttgtt gcataaacaa gttgttcata atgatgaagt attttttctg cctgcaggta
     1381 gaattcatgc tataggaaaa ggattgttaa ttgcagaaat tcaacaaaca tctgatatta
     1441 cctatagaat ttatgatttt aacagaaccg atgaacacgg taatacgaga gaattgcatg
     1501 ttgatttagc tgttgatgtt atagattata aggcttatac agactataag actcattata
     1561 cattaacaca agggccttct tctgaattag tttcgtgtaa gtatttcaca accaatatct
     1621 tgcatttttc aaaacctatt cgtaaaaact attatgaatt tgattcgttt gtaatatata
     1681 tgtgtttaaa aggtgaagct tttattgatt ttggagccga ttatactata cctatacaac
     1741 aaggagaaac agttttaatt ccagcagcat tatacgaaat agaattacta acaagttgtg
     1801 aaacaaaaat aattgaggtt tatattgcgt aatgatacaa ataggaaata ctcttatcag
     1861 tttagatata ctagaaaaaa aatttatatg tgatattaca aagtgtaaag gtatttgttg
     1921 tgtagaaggc gattcaggag ctccgttaac cgatgatgaa accaaagtgt tgtctgaaag
     1981 tcttccgcaa atagaaccat ttttagattc tgttcatgtt gaaattttga aaaaacaggg
     2041 cgtgttttat attgatcatg aaggagaaaa agtaacaact ttagtaaata atgcagaatg
     2101 tgcgtttgtg gttaaaaaag acggcgtatt attttgtgga atagaattag cgtataaaca
     2161 aggattagtt tcaattccta aaccaatttc atgtcattta tatccaatac gttgtaagca
     2221 atatcgcgat tttgaagctc tcaattatga tacttggtat atttgtaaag atgctgtaaa
     2281 taaaggtaat aatgaagggg tagctttata tcaatttctt aaagcaccat taattcgcaa
     2341 atatggagaa cagtggtatg ccgaattaga aatgattgcg caccaatgga agcgttccga
     2401 taaataacat aaatatgagt aactgttaac aatcttttaa taatattttt ttaaccttgt
     2461 gtatatcaat taataaaaaa tagtagtttt acctcgatat tttaacgtga aataaacaaa
     2521 tacaaatagt taaaaatact ttatacatgt caaacaatca acaaattaaa atctttacag
     2581 gaacagcatc gcgttacctt gccgaaaaaa ttgtggcaca ttacggaata gaattaggtg
     2641 attcatcggt tacatttttt agtgatggag agtttgaacc tcgatttgat gtatcagttc
     2701 gtggaaaaga tgtatatatt gttcagtcaa cttttcagcc atcagataat ttaatggaat
     2761 tattgcttat ggttgatgcc gctaaacgag catcggcacg tcaagttaca gcagttatgc
     2821 catattacgg atttgctcgt caggatagaa aagataaatc gcgagtgtca attggggcaa
     2881 aattaattgc aaatttatta acagctgcag gagttgacag ggtagtaacc atggatttac
     2941 atgctggaca aattcaagga ttttttgatg tgccggtaga tcacttatat gcttctacta
     3001 tatttgttcc atatttacga agtttacaaa taccagattt acttatagcg gctcctgata
     3061 tggggggtac caaacgtgca aaatcatatg ctaaattttt gaatgcagat attgctattt
     3121 g
//
LOCUS       DAOTOW010000209         3116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24515_length_3116_cov_2.960144, whole genome shotgun sequence.
ACCESSION   DAOTOW010000209 DAOTOW010000000
VERSION     DAOTOW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24515_length_3116_cov_2.960144"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="P5243_04730"
     CDS             <1..900
                     /locus_tag="P5243_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRS18787.1"
                     /translation="IVLQTNNQTKRISNQIDSILLCFDSVFNNYVSFSQISRINNNDS
                     TVIVHPQFKYFWDKSLYIWNITNGAFDISIAPLVNAWKFGFKNDSLIPDSKKVAELLT
                     LKGMNKMKIHNNRFFKENPRMQIISNAIAQGYSVDIVCRFLDSLQVHNYLVDIGGEMK
                     AKGVNAKGGIWNIGITKPIQNVDSLPVEQSYELSYRLANKALATSGNYRKFYVKDGVT
                     YSHTINPETGYPIRHELLSATVIADECIDADAIATACMVMGLEKSKQFFSKYSQYRCI
                     LLYSLHDSIAVFTNLDKKDINRK"
     gene            1018..1614
                     /locus_tag="P5243_04735"
     CDS             1018..1614
                     /locus_tag="P5243_04735"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS18788.1"
                     /translation="METQIGGDIEKKKELVIIGGPNGSGKTTFTRSYLSMHPMPCLNA
                     DDIAKDINPDDMSKASVSAGKVFFKNLREYQKRGESILIESTLSGAYLNKMIPDFHKQ
                     GYEVIMVYVFLYSPMVCIERIKERVLKGGHHVPDKDVIRRYYRSKENFWNNYKNLADK
                     WFLVYNSENKFIEIALGIKNQFSIKNKEYFYKFVEQFK"
     gene            1645..1827
                     /locus_tag="P5243_04740"
     CDS             1645..1827
                     /locus_tag="P5243_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18789.1"
                     /translation="MNLYLKGFEIESIGNHAVRKVQEENRKKGIPLVYSVDGKIYYEL
                     ADGTVTTNSPFKNDKG"
     gene            1947..>3116
                     /locus_tag="P5243_04745"
     CDS             1947..>3116
                     /locus_tag="P5243_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HRS18790.1"
                     /translation="MNRFLTTVFFVSFFCCNTLFSWAQQSISIDVKADVKPISPLIYG
                     ANNSTTNATAIRWGGNRTTAYNWENNFSNAGEDYNNISDRFFLNGVSTQEQSIPAIPI
                     VNMVRTANTRKQYTLVTLQAAGFVAADANGAVTLEQKAPSSRWDSVVFRKGSTYSLNP
                     DKTDGKVYIDEMVNYLNQSLGAIGSGGIDAFGIDNEPALWGSTHPLIRPNKLTMNELF
                     YKTREVASVVKELSPKADVYGPMFYSWSDMYMLNMDNYVAWNAIKSARGYWGFVDYYL
                     DSLKNVEQEYGKRLLDVLSIHWYPEAKGTSTNLRIVDLIGTSTQAQLTAPDMITARLQ
                     APRSLWDETYIENSYLTSQHGALRLIKRLQNSINQYYPGTKLAFTEFNMMPNIIFP"
BASE COUNT         1121 a          408 c          568 g         1019 t
ORIGIN      
        1 atagtattgc aaacaaataa tcaaaccaaa cgtatttcaa atcaaatcga ttctatatta
       61 ctttgttttg attcagtgtt taataattat gtctcttttt cacaaatttc tcgtatcaac
      121 aataatgatt caacagtaat agttcatcct caatttaaat atttttggga taaatctctc
      181 tatatatgga atataactaa tggagcattt gatatatcta tagcacctct tgtaaatgca
      241 tggaaatttg gttttaaaaa tgattcactt attcctgatt ctaaaaaagt tgcagaattg
      301 ttaaccttga aaggaatgaa taaaatgaag atacacaaca atcgtttttt taaagaaaat
      361 cctcgtatgc aaattattag taatgcaata gcacaaggat attctgttga tattgtgtgt
      421 cggtttttgg attcgttaca ggtacacaat tatttagtag atataggtgg tgaaatgaaa
      481 gcaaaaggag ttaatgccaa aggaggaata tggaatatag gtattaccaa accaatccaa
      541 aatgtggatt cattacctgt agaacaatca tatgaattat catatcgtct tgcaaataag
      601 gcattggcta catcgggtaa ttacagaaaa ttttacgtaa aagatggtgt aacatattcg
      661 catacaatta atcctgaaac cggatatccg atacgtcatg aattattaag tgctacagtt
      721 attgctgatg aatgcattga tgctgatgct attgcaactg cttgtatggt aatgggattg
      781 gaaaagtcaa agcaattttt ttcaaaatat tcacaataca gatgtatttt gctgtattca
      841 ttacatgata gtatagcggt ttttaccaat cttgacaaaa aagatattaa cagaaaataa
      901 acaaaagcac ttttaatcct aacaaatact gagaaaattc gtaggctata gtgtttgaat
      961 ttttatagaa aagaatatat attagataaa aaaaattaag atatttgaaa gatttttatg
     1021 gaaacacaaa ttgggggtga catagaaaag aaaaaagaat tggtaattat aggaggtccc
     1081 aatggttccg gtaaaactac atttacgcgc agctatttaa gtatgcatcc tatgccatgt
     1141 ttaaatgctg atgatatagc taaggatata aatcctgatg atatgagtaa agcaagtgta
     1201 agtgccggaa aagtgttttt taaaaattta agagaatatc aaaaacgagg cgaaagtatt
     1261 ttaatagaaa gtaccttatc gggagcatat ttaaataaaa tgattcctga ttttcataaa
     1321 caagggtacg aagttataat ggtatatgtg tttttatatt cacctatggt atgtatagaa
     1381 cgtattaaag aacgggtgct taaaggtggg catcatgtac ctgataaaga tgtaatacga
     1441 aggtattacc gtagtaaaga aaatttttgg aataattata agaatttagc agataaatgg
     1501 tttttggtat ataattctga aaataaattt attgaaatag cattgggaat aaaaaatcaa
     1561 ttttctataa aaaataaaga atatttttat aaatttgtgg aacaatttaa atgataaact
     1621 atggaggtca aaaaatttgc tgatttgaat ttatacttaa aaggctttga aattgaatct
     1681 ataggcaatc atgccgttag aaaagtacaa gaagaaaatc gcaaaaaagg aataccgtta
     1741 gtgtattcgg ttgatggaaa aatttattac gaattagccg acggtactgt tacaactaac
     1801 agcccattca aaaacgataa agggtaacat atagttacaa ttgtcaataa tgtgaactcc
     1861 ttgattgatt tacatggaat catgtaagtg agtcgaggtt tttttattta tataatttaa
     1921 ggttttattt tactaaacaa tttgttatga atagatttct tactaccgtt ttctttgtaa
     1981 gttttttttg ttgtaatacc ctattttcgt gggctcaaca atcaataagt attgatgtga
     2041 aagctgatgt gaaacctata agtccgctta tatacggtgc aaataattca acaaccaatg
     2101 ctaccgctat tcgctgggga ggtaatagaa ctactgcgta taattgggaa aacaattttt
     2161 caaatgccgg cgaagattat aataatattt ctgatagatt ttttttgaat ggggtaagta
     2221 cacaagagca aagtattcct gcaataccta ttgtaaatat ggtaagaact gcaaataccc
     2281 gaaaacaata tacgcttgtt acgttacaag ctgcaggctt tgttgctgcc gatgcaaatg
     2341 gtgctgttac attagaacaa aaagcaccaa gcagtaggtg ggattctgtt gtgtttcgta
     2401 aaggttctac atattcccta aatccggata aaactgacgg taaagtgtat attgacgaaa
     2461 tggttaatta tttgaatcaa tcgcttggtg caattggaag tggaggtata gatgcctttg
     2521 gtattgacaa tgagccggca ttatggggtt ctacgcatcc attaatcaga cccaataaac
     2581 ttaccatgaa tgaattgttt tataaaacca gagaagtagc aagtgttgta aaagaattat
     2641 cacccaaagc tgatgtatat ggacctatgt tttatagttg gagtgatatg tatatgttga
     2701 atatggataa ctatgttgca tggaatgcaa ttaaatcggc acgcggatat tgggggtttg
     2761 ttgattatta tttagattct cttaaaaatg tggagcaaga atatggtaaa cgattacttg
     2821 atgttttatc tattcattgg taccctgaag caaaaggaac atctacaaat ttacgaattg
     2881 ttgatttaat tggaacatct acacaagctc aattaacagc tcccgatatg attacagctc
     2941 gtttgcaagc tcctcgttca ttatgggacg aaacatatat tgaaaatagt tatttaacaa
     3001 gtcagcatgg agctttgcgt ttaataaagc gattgcaaaa ttctataaat caatattatc
     3061 cgggtacaaa attagcattt accgagttta atatgatgcc gaatatcatt tttccg
//
LOCUS       DAOTOW010000210         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24601_length_3107_cov_3.014744, whole genome shotgun sequence.
ACCESSION   DAOTOW010000210 DAOTOW010000000
VERSION     DAOTOW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24601_length_3107_cov_3.014744"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="P5243_04750"
     CDS             complement(<1..2003)
                     /locus_tag="P5243_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18791.1"
                     /translation="MLNFKRNIFLLIVFNSIALFGYSQNQDSIHTAQIVTSIEKKIAQ
                     AEAAFKSGKVDSTFFYYNRAFAIASDNKLPKWQGEAYHGIGKLFFQLNKIDTAYAVFM
                     KAKEFRKQSNDLIGLNKTLTNLCQIYQQRGQNIEGLAIAKEAIEVGKKANYDRGTGIA
                     YLNAGHFLYHISRYEEALEYYLTASKFFEKAGYDAGIGMCFNSIANIYMNLEKFDFAL
                     EYYKKNLYLQEKIGNTLEIANTCLNLGSFFQDYTRNKSPKPHSNVDSMFYYYNKALTL
                     FKKLQNPIKIIQAYANLGTAYNSVKNYKKAKEYFDIAYNEAIAYNDAIIDIANIEHGY
                     GLMYQGQGNYKEAKKYFLKAFPRIKAANLREKELMWYKDMAINADSLGEYKQAVMYLN
                     KFIGLADTIREQESQKTINQLTMRYSSELKDQEIAAAKERQILMQEKNDEMQKRLYFI
                     IVGLIFIGGLLILVFYSFMQKRKANALLVSRNEEILQQKEEIEVQRNQVLSQKNIIEE
                     QQHNILDSIHYACRIQEAILPQNDTIEELFHENLFVLFKPRDIVSGDFYWLGKKGKKK
                     IVVAADCTGHGVPGAFMSMLGTAFLNEIIGTSDDTITASEILNKLRENVIVSLRQTGK
                     SGEQKDGMDLALFMYNEEDNVIDFAGANNPLIIIRSIQHACAV"
     gene            2216..>3107
                     /locus_tag="P5243_04755"
     CDS             2216..>3107
                     /locus_tag="P5243_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18792.1"
                     /translation="MKTILLSISVLLGAVATVFSQTATIQGNVQTNAAGDQSGIQVVI
                     TGTTYNGLTPVSINQTVNTDAQGNYSFTYNLYTNPLYPAFSNNQYADIMVKLTFNKSG
                     YDSKEILNVTANVTPVTIENTLLFPTGIELYKPTIAMVRYDTLGQYPNVVWERALDKN
                     IKTYIVYRLQGSQWVKAGEVPFTAHGIFEDTDADPSKQYEYQLQVIFEDDSKSDYSAS
                     KQSPKIEVVVENGVPRIHWSSLNEISRIDPLLIRKVLLYRSDSPQGVYEVVDELDISN
                     LTQNELLTKLSMIDKTIPAAG"
BASE COUNT         1062 a          526 c          398 g         1121 t
ORIGIN      
        1 tcaacagcac aagcatgttg tatacttcgt attattataa gcggattgtt tgcccctgca
       61 aaatcaatta cattatcttc ttcattatac ataaacaagg ctaaatccat gccgtctttt
      121 tgttctcccg acttacctgt ttgtcgtaat gaaactataa cattttcgcg aagtttattc
      181 aatatttcac ttgcagttat tgtatcatct gatgttccta ttatttcatt taaaaaagct
      241 gtccctaaca tactcatgaa agctccggga acaccatgac ctgtacaatc ggcagcaaca
      301 acaattttct tctttccttt tttaccaagc caataaaaat caccacttac tatatctcgt
      361 ggtttaaata atacaaataa attttcgtgg aataattctt caatcgtatc attttgaggc
      421 aatatagcct cttgtattct acaagcataa tgaatactat ctaatatatt atgttgctgt
      481 tcttcaataa tatttttttg cgacaaaact tgatttctct gtacctctat ctcttctttt
      541 tgttgcaaaa tttcttcatt tctactcact aacaaagcgt ttgctttgcg tttttgcata
      601 aatgaataaa atacaagaat aagtaaccct ccaataaata ttaaacctac tataataaaa
      661 tacaaacgtt tttgcatctc atcatttttt tcctgcatta gaatttgacg ttcttttgca
      721 gctgcaattt cttgatcttt caattcagaa ctataacgca tagtaagctg attaatagtt
      781 ttttgcgatt cttgctctcg aattgtatct gctaaaccaa taaatttatt tagatacata
      841 actgcttgtt tatattctcc aagtgaatca gcattaatag ccatatcttt gtaccacata
      901 agctcttttt cacgtaaatt agctgctttt atacgcggga aagccttcaa aaaatacttt
      961 ttggcttctt tataatttcc ctgcccctga tacattaatc catatccatg ttcaatattt
     1021 gcaatatcaa taattgcatc attatatgca atggcttcat tatatgctat atcaaaatat
     1081 tcctttgctt ttttataatt tttaactgaa ttgtaagctg tacctaaatt tgcatacgct
     1141 tgaattattt ttattggatt ttgtagtttt ttgaatagtg taagagcttt attataataa
     1201 taaaacatag aatcaacatt agagtgtggt tttggacttt tatttctggt ataatcctga
     1261 aaaaaagaac caagattcaa acaagtattt gcaatttcta aggtattacc tattttttct
     1321 tgtaaataca gatttttctt ataatattct aaagcaaaat caaatttttc tagattcata
     1381 taaatatttg caatactatt aaagcacata ccaataccgg catcatagcc tgctttttca
     1441 aaaaatttac tggcagttaa atagtactcc aaagcctctt catatctgct tatatgatat
     1501 aaaaaatgtc ctgcatttaa atatgctata ccggttcctc tgtcataatt tgctttttta
     1561 cctacctcta ttgcttcttt tgctatggca agaccttcaa tattctgacc tctttgctgg
     1621 taaatttgac acaaattagt aagcgtttta ttcaatccta ttaaatcatt tgattgtttt
     1681 ctaaattctt ttgctttcat aaaaacagca tatgctgtat caattttatt taattgaaaa
     1741 aataattttc caattccatg atatgcttcg ccttgccact ttggcaattt attgtctgat
     1801 gcaattgcaa atgcacgatt atagtaaaaa aatgttgaat caactttacc ggatttaaaa
     1861 gcagcttctg cttgagctat ttttttttct attgatgtta caatttgtgc tgtatgtata
     1921 gaatcttgat tttgagaata cccaaataaa gcaatactat tgaatactat aagtaaaaaa
     1981 atatttcttt taaaattgag catcttcata aaccaaataa actttataca aagataaaat
     2041 ttttgttaca ataacaaacg aaaaaaaaca ctattgacaa ttttcacaaa aaacacccta
     2101 tactgtttta tatttgtttt tcagtgagtt atgttattaa aaaaataaaa aaaatattat
     2161 gattttattc atttatgata tatatttaca ctataaacat aaaaaataag gagatatgaa
     2221 aacaatatta ttaagtataa gcgtattgct tggtgctgta gctactgttt tttcacaaac
     2281 agctaccatt caaggaaatg ttcaaacaaa tgcagcagga gatcaatcag gaatacaggt
     2341 tgtaattaca ggaacaacat acaatggatt gactcctgtt agtataaatc aaactgtaaa
     2401 taccgatgct caaggcaatt acagttttac ctataattta tatacaaatc ctttgtatcc
     2461 tgcattttct aataatcaat atgcagatat aatggtaaaa cttacgttta acaaaagtgg
     2521 atacgactct aaagaaattc taaatgttac agcaaatgta actcctgtaa caatagaaaa
     2581 cactctatta ttcccaaccg gaattgaatt gtataaacct actattgcaa tggtacgata
     2641 tgatacatta ggacaatatc caaatgtagt ttgggaacgt gctttagata aaaacatcaa
     2701 aacctatatt gtgtatagac tacaaggttc acaatgggta aaagcaggtg aagtcccatt
     2761 tacagctcat ggtatatttg aagataccga tgctgaccca tcaaaacaat atgaatatca
     2821 actacaagta atatttgaag atgactctaa atctgactat agtgcatcaa aacaatcacc
     2881 taaaatagaa gtggttgttg aaaatggtgt tcctcgcatt cattggtcaa gtttaaatga
     2941 aatttctcgt attgaccctc tgcttattcg aaaagtgtta ttatacagaa gcgattctcc
     3001 tcaaggtgta tatgaagttg ttgacgaatt agacatttca aatctaactc aaaatgaatt
     3061 acttactaag ttaagtatga tagataaaac tatacctgca gcaggca
//
LOCUS       DAOTOW010000211         3097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24695_length_3097_cov_3.327416, whole genome shotgun sequence.
ACCESSION   DAOTOW010000211 DAOTOW010000000
VERSION     DAOTOW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24695_length_3097_cov_3.327416"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1269)
                     /locus_tag="P5243_04760"
     CDS             complement(109..1269)
                     /locus_tag="P5243_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HRS18793.1"
                     /translation="MAYSFDTIISREHTNCYKYDLRSKVFGDSSVIPLWVADMDFKIA
                     DEIIEDITKAAEHGIYGYTYFYEELYNSLIQWNARRNNWHFSASDVVFYHGIVPSLNL
                     IIQEFTNVGDEIIVQTPVYFPFFESVEKHSRVVLNNQLVLRDGVYQIDFDALERSISP
                     RTKLMILCHPHNPVGRCWTHQELMQLHDICKKHSILVVSDEIHADILLYDNKHIPWAT
                     LSDYARENSITLVAPSKTFNIAGLSISAIVCQNKQLLITIKSFIERNQLHGMSPLNMK
                     AFESAYTKGEQWLFELLRYLEGNLQCIREVMQQTSKIYLIEPQATYLAWLDCRNMNMT
                     DGELQSFFVRKAGVGLSRGIQFGSGGGGFMRLNYACPRSVLLQALHQITNAL"
     gene            complement(1274..2500)
                     /locus_tag="P5243_04765"
     CDS             complement(1274..2500)
                     /locus_tag="P5243_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS18794.1"
                     /translation="MGFTTKVLHTKPNKHDAYGALRFPAYANAAFEFASAEDIEAAFK
                     GTKPTHAYSRSSNPTIEYFEKTILNVTGALACIGFSSGMGAISSCLLALCKAGDEIVS
                     SNKLFGTTYSLFTDTFAEYGIVVKCVDFNSIENVRAAISDKTRAIFFETITNPQIEIY
                     AIDELVAIANAHAIPLVADTTATPPYMFDAKLHGIHISVLSSTKFISGGGTAIGGLLI
                     DNGLYDWKQHVNFAEQAIKYGAFSFIRKLRLLTYRNLGACMSVQAAQLFSLGLETMEL
                     RIDRACSNAQVVAEFLEQHPRVKHVRYAGLTTNKYFAKAQKFFTYPGSIICFELASKQ
                     DCFSLINAVQLIRRATNLHDNKTLVIHPSSTIYSEFPLEKQAEIGVVDTMIRISIGIE
                     NSEDIIADLSQALSQL"
     gene            complement(2510..>3097)
                     /locus_tag="P5243_04770"
     CDS             complement(2510..>3097)
                     /locus_tag="P5243_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRS18795.1"
                     /translation="EIVLTPAPEGMKGAIAKANALQEELCNVFIPQQFQNPANPEIHR
                     KTTAIEIWNDTDGTVDFIVAGVGTGGSITGISEVIKAKKPSCKAIAVEPDDSPVLSGG
                     KPGPHKIQGTGAGFVPEILNTSIIDEIVRVTNDQAFDTAREIAKQEGIFCGISSGAAV
                     YAALQVAKRAENKGKTFVVILPDTGERYLSTTLFD"
BASE COUNT         1036 a          608 c          487 g          966 t
ORIGIN      
        1 aaatctcatt cccggcaaag gatattatat taaaaagaaa tagtttttat ataccacaga
       61 ataatcatac atacacatcc cgaaagaatg ataggctttc gggatttttt ataaagcatt
      121 tgtaatttga tgtaatgctt gtaataaaac agatcgagga caagcataat tgagtcgcat
      181 aaacccaccg cctccactac caaattgaat tccacgacta agacctactc ccgcttttcg
      241 cacaaaaaat gattgtaatt ctccatctgt catattcata tttctacaat ctaaccaggc
      301 taaatatgtt gcttgaggtt ctataagata tatttttgat gtttgttgca taacctctcg
      361 tatgcattgt aaattgcctt ctaaatatcg caacaattca aaaagccatt gttctccttt
      421 agtatatgcc gattcaaatg ctttcatatt taatggactc atgccatgca attgattacg
      481 ttcaataaat gacttaattg ttataagcag ttgtttattt tgacacacaa ttgccgaaat
      541 tgataagccg gcaatattaa aagttttact gggagcaact aacgttatag aattttcgcg
      601 agcataatca ctcaatgtag cccagggaat atgtttatta tcatacaata aaatatcagc
      661 atgaatttca tcagaaacaa ccaaaatact atgtttttta catatatcat gcaattgcat
      721 aagctcttga tgagtccaac atcgtcctac aggattgtga ggatgacaaa gaatcataag
      781 ttttgtacgt ggactaatac tccgttccaa tgcatcaaaa tctatttgat acactccatc
      841 acgcaaaacc aattggttat tgagaacaac cctagaatgt ttttcgaccg actcaaaaaa
      901 aggaaaatac acaggggttt gaacaattat ttcatcacca acattagtaa attcctgaat
      961 aataagattt aacgaaggaa caataccatg atagaacact acatcagatg ctgaaaaatg
     1021 ccaattgttt ctacgtgcat tccattgtat taatgaatta tataattctt cataaaaata
     1081 ggtatatcca tatattccat gttcagcagc ctttgtaatg tcttcaataa tttcatcggc
     1141 aattttaaaa tccatatcgg caacccacaa aggaattaca gaagaatcac caaacacttt
     1201 agaacgcaaa tcatatttat aacaattggt atgttcgcga gaaataattg tatcaaaaga
     1261 atatgccata gtattataat tgagataatg cttgagacaa atcagcaata atatcttctg
     1321 aattttcaat acctattgaa attcgaatca tagtatcgac cacccctatt tcggcttgtt
     1381 tttcaagtgg aaactcacta tatattgttg atgacgggtg aattactaac gttttgttgt
     1441 cgtgtaaatt tgtcgctcta cgaattaatt gcaccgcatt tatcaatgaa aaacaatctt
     1501 gtttcgaagc taactcaaaa caaataattg aaccgggata cgtaaaaaac ttttgagctt
     1561 tggcaaagta tttattggtt gtcaatcctg catatcgaac atgcttcacg cgtggatgtt
     1621 gttccaaaaa ttcagcaaca acttgtgcat tactacaagc acggtcaatt cgcaactcca
     1681 tagtttctaa accaagtgaa aacaattgtg ccgcttgtac cgacatgcat gcaccaagat
     1741 ttctatacgt tagcaaccgt aatttacgaa taaatgaaaa tgcaccatat tttatagctt
     1801 gttcggcaaa attcacatgt tgtttccaat cgtacaaacc attatctata agcaacccac
     1861 caatggcagt acctccaccc gaaataaatt tagtagatga aagcaccgaa atatgaatgc
     1921 catgcaattt agcatcaaac atatacggcg gtgtagcagt agtatcggct accaaaggaa
     1981 ttgcatgagc atttgctata gcaacaagtt catctattgc atatatttca atttgaggat
     2041 tggtaatagt ttcaaaaaat atagcacgag ttttatctga aattgcagct ctcacattct
     2101 ctattgaatt aaaatcgaca cactttacga cgataccata ttctgcaaat gtatctgtaa
     2161 ataaagaata cgtagtacca aacaatttat tggaactcac aatttcatcg ccggctttac
     2221 acaaagctaa caagcacgat gaaattgctc ccatacccga tgaaaagcca atacaagcta
     2281 aggcaccggt aacatttaat atagtttttt caaaatattc tatcgtagga tttgacgaac
     2341 gtgaataggc atgtgtaggt tttgtacctt taaaagcagc ctcaatatct tcggcacttg
     2401 caaattcaaa cgcggcgttt gcgtatgcag gaaatctaag agctccatac gcatcatgtt
     2461 tatttggttt tgtatgtaaa accttagtag taaaccccat gcactataat taatcaaaaa
     2521 gtgttgtact taaatatcgt tctccggtat caggtaatat aacaacaaat gttttacctt
     2581 tattttcggc acgtttagca acttgcaagg cagcatacac agctgctccc gaagaaattc
     2641 cgcaaaaaat cccttcttgt tttgcaattt cgcgagctgt atcaaatgct tgatcgttgg
     2701 ttacgcgaac aatttcgtca ataatagacg tatttaaaat ttcgggcaca aaccctgctc
     2761 cggtaccttg aattttatga ggccccggct taccacctga aagtacgggt gaatcatcgg
     2821 gctctacagc aattgcttta catgatggtt ttttagcttt tattacctca ctaattccgg
     2881 taatagaacc tcctgtacca acacctgcaa caataaaatc aactgttccg tcagtatcat
     2941 tccaaatttc tattgcagta gtttttctat gtatttcggg atttgcggga ttttgaaact
     3001 gctgaggaat aaatacatta cacaattctt cttgtaatgc atttgcttta gctatagcac
     3061 ctttcattcc ttcgggagcc ggagttaaca ctatttc
//
LOCUS       DAOTOW010000212         3090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24761_length_3090_cov_2.926524, whole genome shotgun sequence.
ACCESSION   DAOTOW010000212 DAOTOW010000000
VERSION     DAOTOW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24761_length_3090_cov_2.926524"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="P5243_04775"
     CDS             complement(<1..742)
                     /locus_tag="P5243_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18796.1"
                     /translation="MKKCLLTIAAGLLIGFGASAQYDATIWQVEDATTKVYSLTGLTK
                     TNGFDTETSGSTVSTGYDDTYTYTWETNDIIKIDFTNKLTPNQWSDFGFTVVQWDGTT
                     NNKFTKTTAGADQDHDKARGWTVDFSDEANRLVTMKVQASANINLRADLTDIAGKTSN
                     GASPKVDVVATTGGVNINDAAKWATIKYSWGGAMVDAGAAAVEIMEDWYSKTWWGVDN
                     AGSKDAQNPLDSARIVKLGLTFDDGGQGT"
     gene            complement(843..>3090)
                     /locus_tag="P5243_04780"
     CDS             complement(843..>3090)
                     /locus_tag="P5243_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HRS18797.1"
                     /translation="KWDPINNDFGGAAYKLYLNYNNEGGRFGNTSVRATTTNNAAIST
                     FASLDENNNLHVVVINKTSSQQTTQFGIANGYYSHGQVYGFDQTDKTIREFAAVSSIS
                     NSGFEYALPAYSALHFVLYAKPQVQVLAARILPANASQIVLKCSQSVEVTDPAQAIAE
                     FTVSNTAGTNLIVTNIVASQNFLIVTLNNPIDALDSLCTISFSGISIVGDNQLPIASF
                     QSIPVYNLQESSPLFVRNATVVYNGKSIVLNLSKQITDDAQNSNGILLQVNSQTLAID
                     SIRLSDQGFSLVLYPSQRILKEQIVQLTSTIEDIESTDGKVISKFSVTATNNGPNVSP
                     AIDSIVIKNNFSFTAFVNNRLDATNFTNAGFVLYEDGVSIPFTATYVNQQIVFTTQSP
                     MFKEKVYTLSYVDTDKIMTIFGGYLNGFENASLINRLKPTPDYIAIPSQIEAEDYAYH
                     KSSSVLESTNDIGGGVHVGFIGTNDIYAYHITVPETKNYTVTLRHSGASQDGTVLFKV
                     NGVTKARMFVPKTGSWNVWKNSSVVLPLESGDITIEMHVLATGFNANWLSFAEGENPS
                     NALLVLSQTDNAGENVYLFFDRKLRVLPQISEIQLTNNSAPLQITNISYVGNDSTKLQ
                     LQIQGIIYKNNVITVTYNQTSAVTTENGILQPVSSMQVSNKSTQINSQIELIQAGITI
                     LNVNNTICIESVDNIEYTYSIYTIHGKLISKGVGYRNTSISIEKHGIYVVEVETVEQK
                     ICKKIRIE"
BASE COUNT         1006 a          544 c          459 g         1081 t
ORIGIN      
        1 atgtaccttg tccaccatca tcaaaagtta aacctaattt tacgattctc gcagaatcaa
       61 gtggattttg agcatctttc gaaccagcat tatcaacacc ccaccatgtt ttagaatacc
      121 aatcttccat aatttctaca gcagcagcac cagcgtcaac cattgcacca ccccaagaat
      181 atttaatagt tgcccattta gcagcatcat taatatttac accacctgta gtagctacaa
      241 cgtctacttt tggagacgca ccattacttg ttttacctgc aatgtcagtt aagtcggcac
      301 gtaaattaat gtttgcagaa gcttgcactt tcatagtaac caaacgattt gcttcgtcag
      361 aaaaatctac tgtccaacct cttgctttgt catgatcttg atctgcacct gcagttgttt
      421 tagtaaattt gttatttgtt gttccatccc attgtacaac tgtgaaaccg aaatctgacc
      481 attgatttgg agttaatttg ttagtgaaat caattttgat aatgtcatta gtttcccaag
      541 tataggtata tgtatcatca tatcctgtac ttacagttga accagaagtt tcagtgtcaa
      601 aaccgttagt ttttgttaaa cctgttaaag agtaaacttt tgttgttgcg tcttcaactt
      661 gccaaattgt tgcgtcatac tgcgcagatg ctccgaaacc gattaataat ccggcagcaa
      721 tagtaagtaa acattttttc atagtaaaaa atattaagtt tataaataca ttaattaaaa
      781 tttattaata cgcaaatata caaataataa ttaagatgca agtaatttcg ttaaaaaatt
      841 agttattcta tacgtatttt tttacatatt ttttgttcca cggtctcaac ttctaccaca
      901 tatattccgt gtttttcaat actaatactc gtattcctat agccaacacc ttttgaaata
      961 agctttccat gtatggtata tatactatat gtatattcta tattatctac agattctata
     1021 catattgtgt tatttacatt taaaattgtt atacctgcct gtattaattc tatttgagaa
     1081 ttgatttgtg tagacttatt ggaaacttgc atactcgaaa ctggttgtaa aataccattt
     1141 tcagttgtaa cagccgatgt ttggttatat gttactgtaa ttacattatt tttatatatt
     1201 attccttgta tctgcaattg caatttagta ctatcatttc ctacgtagct tatatttgta
     1261 atttgcaacg gtgcagaatt atttgttaat tgtatttcag aaatttgagg taatactcgt
     1321 aattttctgt caaaaaataa atatacattt tcgccggcat tatcagtttg cgagagcact
     1381 aacaaggcat tactcgggtt ttcaccttcg gcaaacgata accagttagc attaaatccc
     1441 gttgcaagca catgcatttc tatagtaata tctcctgatt ctaatggcaa caccaccgat
     1501 gaatttttcc atacattcca cgaacctgtt ttgggaacaa acatacgagc ttttgtaacg
     1561 ccatttactt taaacaatac tgttccatct tgacttgcac cggaatgtcg caatgtaact
     1621 gtataatttt ttgtttcggg tacagttata tgatacgcat atatatcatt ggttcctata
     1681 aatccaacat gtacacctcc acctatatca tttgttgatt caagtaccga tgaactttta
     1741 tgatacgcat aatcttcggc ttctatttgc gatggtatag caatataatc aggtgttggt
     1801 tttaatctat ttataagtga tgcattttca aatccattta aatacccgcc aaaaattgtc
     1861 ataattttat cagtatctac atacgaaagt gtatatactt tttctttgaa cataggcgat
     1921 tgtgtggtaa aaacaatttg ttgatttaca tatgttgcag taaaaggtat agaaacacca
     1981 tcttcatata acacaaaacc tgcatttgta aaattagtag catctaacct attatttaca
     2041 aaagctgtaa acgaaaaatt atttttaatc actatagaat ctattgcagg cgatacattt
     2101 ggtccattat tagttgctgt aacagaaaat tttgaaatta ctttaccatc agttgattct
     2161 atatcttcaa tagttgatgt aagttgaact atttgttctt tcaaaattcg ttgtgaagga
     2221 tacaatacta atgaaaaacc ttgatctgac aaacgaatag agtcaattgc aagagtttga
     2281 gaattaactt gtaacagaat accattagaa ttttgagcat catctgttat ttgttttgat
     2341 aaattcaaca ctatagattt accattatat acaactgttg cattacgaac aaataaaggc
     2401 gaagattctt gtaaattata taccggtata gattgaaacg atgctatagg taattggtta
     2461 tctcccacta tagatattcc tgaaaacgag atagtacata aagaatcaag tgcatctatt
     2521 ggattattaa gagttacaat aagaaaattt tgagatgcaa caatattagt aacaataaga
     2581 ttagttcccg ccgtattaga aaccgtaaat tcagctattg cttgggcagg atctgtaacc
     2641 tcaacagatt gagaacattt cagtacaatt tgcgatgcat ttgccggaag aattcttgct
     2701 gctaatactt gcacttgagg tttggcatac aatacaaaat gcaaagctga atatgcaggc
     2761 aaagcatact caaatcctga atttgaaata cttgaaactg cagcaaattc acgaatagtt
     2821 ttatcggttt ggtcaaatcc atacacctga ccatgtgaat aatatccatt tgctatacca
     2881 aattgagtag tttgctggct cgaagtttta ttaataacaa caacatgtaa attattgttt
     2941 tcatctaacg atgcaaatgt tgaaatagca gcattattag ttgttgtagc gcgaactgaa
     3001 gtattaccaa atctaccgcc ttcattatta taattaagat acaacttata tgcagcacct
     3061 ccaaaatcat tattgatagg atcccactta
//
LOCUS       DAOTOW010000213         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24856_length_3082_cov_3.609184, whole genome shotgun sequence.
ACCESSION   DAOTOW010000213 DAOTOW010000000
VERSION     DAOTOW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24856_length_3082_cov_3.609184"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="P5243_04785"
     CDS             <1..1611
                     /locus_tag="P5243_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRS18798.1"
                     /translation="PKQFTYPFCYTPHNVCLEAIKQLQDVVTSYSHWNHDFGIDHFVE
                     GSNVGKMFGVLVVQNSAHEIGFLAAFSGKIGESQVYPHFVPPISNIMESGSFYRTGED
                     ELKHINDAIYTMQQSVEYTRAIQEYTTICKQAELELATKKQEIANNKLQRKAIRKTIA
                     QTHDQEIILHQLAKQSSDEHFAYKQLLAFWNKRKEEAQKHVDFFEIQIQQLKEERKRK
                     SAALQQQIFDTYNFLNARGETANVREIFAHTEVGVPPAGAGDCAAPKLLQYAYMHAYK
                     PIAMAEFWWGQSPKSEIRKHLHTYPSCKAKCEPILGFMMQGLDVEPNPILQKLAKHKV
                     PEIVYEDDALLVINKPADMLSVPGKIEAESVYTYFQAQYDFPLYMVHRLDMSTSGILI
                     IAKSLEVFTALQKQFTLRTIRKQYVAIVEGVVAQDSGTIQLPLRVDLDNRPSQIVCFA
                     HGKPAITTWKVVKRMHNATRIHLYPHTGRTHQLRVHCAHSQGLNCPIRGDELYGTPAD
                     RLYLHAQYIHFEHPTTKKKMSFTVEADF"
     gene            complement(1648..>3082)
                     /locus_tag="P5243_04790"
     CDS             complement(1648..>3082)
                     /locus_tag="P5243_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18799.1"
                     /translation="LLAGFEAEAVTKNIASMKASNFPYEDIASLFAASKMEGIDSKQY
                     ESALLSGFSNAQYNFKSTYFVSASVRSDGSSKFSPGHRWGTFWSAGGSWIASNEEFFK
                     QFSSVSFAKLRASYGTSGNANIQDYAYMQLYEFAKNYNDEPGSVPIQLGTNALTWEKN
                     NTANIGVDVQIRKRIDISLDAYHRKTYDLLLLVPIPSISGYTHQFQNVGAMTNKGIEC
                     MLQVQVYKKDFLQYTTSVNVAHNVNTITELYSPDINDTIIQGTKIRTEGHSFQSFYLA
                     EWAGVNSADGSPMWYDANGNLTKDYNKARKILAGTADPKLTVGWNNTVQYKQWSCSMM
                     WYYNYGNLIFNQLQTELQSDGALWGKNQSIQALDYWKAPGDNAINPKLVKNNSSNSNE
                     FSTRYLESGSYLRLKQIQVSYTIQPKEVSKAAHMRVQNIQFTVQANNVWVLSSFTGLD
                     PETRANGVYYFDYPKQRLFSGGVSVKF"
BASE COUNT          980 a          614 c          542 g          946 t
ORIGIN      
        1 cccaagcaat ttacctatcc attttgctat actccccata acgtatgttt agaagctatt
       61 aagcaattac aagatgttgt aacttcttat tcccattgga atcatgactt tggaattgat
      121 cattttgtag aaggttctaa tgtaggcaaa atgtttggtg tactggtagt acaaaacagt
      181 gcacatgaaa ttgggttttt ggcagcattt tcaggtaaaa taggcgaatc tcaggtatat
      241 ccacactttg ttcctcctat atcaaacatt atggaatcgg gcagttttta tcgcacaggc
      301 gaagacgaat taaagcatat aaatgatgct atatatacca tgcaacaatc tgtagagtat
      361 acacgtgcca tacaagagta tacaactatt tgcaaacaag cagaactaga attagcaaca
      421 aaaaaacaag aaatagcaaa taataaatta caaagaaagg ctatacgaaa aacaatagca
      481 caaactcacg accaagaaat tattttacac caattggcaa aacaaagcag cgatgagcat
      541 tttgcatata aacaactgct tgcattttgg aacaaacgca aagaagaagc acaaaagcac
      601 gtagattttt ttgaaataca aatacagcaa ttaaaagagg agcggaaacg taaatcggcg
      661 gccttgcaac agcagatttt tgatacctat aattttctaa atgctcgcgg cgaaacagcc
      721 aatgtgcgcg aaatttttgc acatactgaa gtaggtgttc ctcctgcagg tgcaggtgat
      781 tgcgctgctc ccaaattatt gcagtatgca tatatgcatg cgtataaacc tatagctatg
      841 gcagaatttt ggtggggaca atcgccaaaa tctgaaatac gaaaacattt acatacgtac
      901 ccctcatgca aagctaaatg cgaacctata cttggattta tgatgcaagg attagatgtg
      961 gaacccaatc caatactgca aaaattggca aaacataaag tccccgaaat tgtgtatgaa
     1021 gacgatgctc tattggtaat aaacaaacct gccgatatgc tctcggttcc gggtaaaata
     1081 gaagctgaat cggtttatac ctattttcaa gctcaatatg attttcccct atacatggtt
     1141 cataggcttg atatgtcaac atcgggcatt cttattattg caaaatcgct cgaagtgttt
     1201 accgcattgc aaaagcaatt tacacttcgt accatacgca agcaatatgt tgctatagtc
     1261 gaaggtgtag ttgcacaaga ttcaggcacc atacagttac ctctgcgtgt tgatttagac
     1321 aatcggccat cgcaaatagt gtgttttgca catggtaaac ccgcaattac aacgtggaaa
     1381 gttgtaaaac gtatgcataa tgcaacacgc attcacctct atccacacac agggcgtacc
     1441 catcaattac gtgttcattg tgcgcactca caaggcttga attgtcctat tcggggcgat
     1501 gaattatacg gcactccggc cgacagattg tacctgcatg cacaatacat tcattttgaa
     1561 catcctacaa ctaaaaagaa aatgagtttt accgttgaag ctgattttta actatacgtt
     1621 gtatcttttt ttattacaca caatatatta aaactttaca ctaactccgc cactaaacaa
     1681 tcgttgttta ggataatcaa aatagtatac tccattagca cgggtttcgg ggtcgagtcc
     1741 ggtaaatgac gaaagtaccc atacattatt tgcctgcaca gtaaactgaa tgttctgtac
     1801 tctcatgtgt gctgcttttg atacttcttt gggttgtatg gtgtacgata cctgtatttg
     1861 tttgaggcgg aggtaactgc cgctttctaa atagcgggta gaaaattcat tactattact
     1921 gctattgttt tttacaagtt taggatttat tgcattatcg ccgggggctt tccaatagtc
     1981 taacgcttgt attgattgat ttttccccca caaagcacca tcactttgca actctgtttg
     2041 caattggtta aatataaggt ttccgtaatt ataataccac atcatactac atgaccactg
     2101 cttgtattgc acggtattgt tccaccctac tgtaagttta ggatcggctg ttcctgccaa
     2161 tatcttacgt gccttgttat agtctttggt taaattacca ttggcatcat accacatagg
     2221 cgaaccgtca gcagaattta cgccggccca ttctgccaaa taaaaacttt gaaacgaatg
     2281 accttcggtt ctgatttttg taccttgaat aatagtatca tttatatcgg gcgaatataa
     2341 ttcggtaatt gtattaacat tgtgagctac atttacgctt gtagtatatt gcaaaaaatc
     2401 ttttttatat acttgaacct gaagcataca ctctattcct ttatttgtca ttgcccctac
     2461 attttgaaat tgatgtgtat atccgcttat acttggtata ggaacaagta ataacaaatc
     2521 atatgtttta cgatgataag cgtcaagcga tatgtcaatt cgtttgcgaa tttgcacatc
     2581 aacaccaata tttgcagtat tattcttttc ccatgtaaga gcattggttc ctaattgtat
     2641 tggtacacta cccggttcat cattataatt ttttgcaaat tcatataatt gcatatatgc
     2701 ataatcttga atatttgcat tgcccgatgt accataactt gcacgtaatt tggcaaatga
     2761 taccgacgaa aattgtttaa aaaattcttc atttgatgct atccatgaac cacctgccga
     2821 ccaaaaggtt ccccaacgat gccccggact gaatttagaa cttccatcgg aacgtacact
     2881 tgcacttaca aaatatgttg atttaaaatt atattgtgca tttgaaaaac ccgacaacaa
     2941 agcactttca tactgcttac tgtcaatacc ttccattttg gaagcagcaa ataacgaggc
     3001 aatatcttcg taaggaaaat tagacgcctt catagaagca atattttttg taacggcttc
     3061 ggcttcaaaa cctgctaata at
//
LOCUS       DAOTOW010000214         3076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24919_length_3076_cov_2.835485, whole genome shotgun sequence.
ACCESSION   DAOTOW010000214 DAOTOW010000000
VERSION     DAOTOW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24919_length_3076_cov_2.835485"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="P5243_04795"
     CDS             <1..97
                     /locus_tag="P5243_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRS18800.1"
                     /translation="KAEIKLNRKVLADLAMNHPKAFEAIVKAVKK"
     gene            168..890
                     /gene="dapB"
                     /locus_tag="P5243_04800"
     CDS             168..890
                     /gene="dapB"
                     /locus_tag="P5243_04800"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRS18801.1"
                     /translation="MNIALIGYGKMGKEIEQIAIERGHTIALIIDITNQQDLNVTNLH
                     NKKIDVAIEFTIPDSAFSNYSICFEADVPVVSGTTGWLHKFNDVVEICETQNKGFFYA
                     SNFSLGVNIFFALNKRLAQLMNQFNQYDVVLEEIHHTQKLDAPSGTAITLAEDIVKHL
                     DRKKTWQLNEFTSTDNLQILAKRLGKIPGTHTITYDSDVDSIEISHVAKSRKGFATGA
                     VLAAEFMKGKTGIYGMKDLLGI"
     gene            902..2308
                     /gene="lepB"
                     /locus_tag="P5243_04805"
     CDS             902..2308
                     /gene="lepB"
                     /locus_tag="P5243_04805"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRS18802.1"
                     /translation="MKKIFENKWVLFATIATAYLLWVIWLGNFWWLLGIGIIFDLYIT
                     KKVPWAFWKIYKPKNKFQKWLLGWVDAIIFAVIAASFIRLFFFEAYTIPTSSMEGTLL
                     VGDFLFVSKFHYGPRPQMTPLSFPFVHHSMPFSNNMTKSFSTAWQLPYKRMAGLQTIK
                     NDDIVVFNFPEGDSVIVTRQSQSFYQLKRDFPNCLYPLSPRDFQIYNKLKMQYPTDEA
                     CFYAYFKDSIVYRPVDKRENYIKRCVAIAGDTLQVKGGMVFINGKPQKSIPGIQFRYD
                     IFTSGLKLRESIINDIGIREYGYTANGYTMLMNNEQYTKLKKISIISEIVKAEMREGQ
                     WYRPCFPQHINYPWNKDFFGPLVIPKAGTTIQLTTENLPLYERIIDVYEGNDLRVDGI
                     DIYINNVKTSTYTFKMNYYWMMGDNRDNSQDSRFWGFVPEDHIVGKASLIWLSLNDKG
                     VFPFNIRFNRLFTVIHKN"
     gene            2318..2926
                     /locus_tag="P5243_04810"
     CDS             2318..2926
                     /locus_tag="P5243_04810"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRS18803.1"
                     /translation="MLLSTAYMPCIEYMAYIAQSDCVTIEAHEHYIKQSYRNRAYIAT
                     ANGIMELIIPVIQPNGSKTHIRDVQISYAEPWQKKHWHAIVSAYNSSPYFEYYQYLFA
                     PFYTQKFTFLWDYNNQFLSIILKLFGITTSILYSNSFTPLHTIEHDKRYTLTPKHIST
                     YKTPKYPQVFEEKHGFVPNLSCIDLLCNVGPEAKKNVLYMGM"
BASE COUNT         1088 a          534 c          475 g          979 t
ORIGIN      
        1 taaagcagaa attaaattga atcgaaaagt tttagcagat ttagctatga atcacccaaa
       61 agcattcgaa gctattgtaa aagcagtaaa aaaataaatc atattttttt tatatttggg
      121 gaagctttat gcttcccttt tttttttata acatttacat atacaatatg aacatagcac
      181 ttataggata cggaaaaatg ggcaaagaaa ttgaacaaat tgccattgaa cgtggtcata
      241 ctattgccct cataattgac attacaaatc aacaagattt aaatgttacc aatctccaca
      301 ataaaaaaat agatgttgca atagaattta ctattcctga ttcagcattt tctaattatt
      361 ctatttgctt cgaagcagat gttccggtag tttcgggcac tacaggttgg ttacacaaat
      421 tcaatgacgt agttgaaata tgcgaaactc aaaacaaagg attcttttat gcttctaact
      481 ttagtttagg tgtaaatata ttttttgctc ttaataaacg cctggctcaa ttaatgaatc
      541 aattcaacca atacgatgtt gtattagaag aaattcatca tacccaaaaa cttgacgcgc
      601 caagtggtac cgctataact cttgccgaag atattgtaaa acacttagat cgcaaaaaaa
      661 catggcaatt aaatgaattc acttcaaccg ataatttaca aatacttgca aaaagactcg
      721 gaaaaattcc cggcacacat acaataacat acgattctga cgttgatagc attgaaattt
      781 ctcatgtagc aaaatctcga aaaggatttg ctaccggagc cgtattagcc gccgaattta
      841 tgaaaggtaa aaccggaata tacggaatga aagatttatt aggaatataa tacattcata
      901 catgaaaaaa atttttgaaa acaaatgggt gctttttgct actatagcaa cagcatattt
      961 attatgggta atttggttag gtaatttttg gtggttgctt ggcataggaa tcatctttga
     1021 tttatacatc acaaaaaaag ttccatgggc tttctggaaa atatataaac cgaaaaataa
     1081 atttcaaaaa tggctacttg gttgggttga tgcaataatt tttgctgtaa ttgctgcttc
     1141 ttttattcgt cttttctttt ttgaggcata tactatacct acttcatcta tggaaggtac
     1201 attacttgta ggcgattttt tatttgtgag caaatttcat tacggaccac gtcctcaaat
     1261 gacaccactg tcatttcctt ttgtacacca ttctatgcca ttttccaaca atatgacaaa
     1321 atcgtttagc acggcgtggc aacttcctta caagcgaatg gccggtttac aaactataaa
     1381 aaatgatgat attgtagtat ttaattttcc cgaaggcgat agcgttattg taacacgcca
     1441 aagtcaaagt ttttatcaat taaaacgcga ttttccaaat tgcttatatc ctctttcacc
     1501 aagagatttt caaatatata acaaacttaa aatgcaatat cctaccgacg aagcatgttt
     1561 ttatgcatat tttaaagatt ctattgttta tcgccctgta gacaaacgcg aaaattatat
     1621 aaaacgatgt gttgctatag ccggtgatac attacaagta aaaggcggaa tggtattcat
     1681 taacggaaaa cctcaaaaat ctattcccgg aattcaattt agatatgaca tttttacatc
     1741 gggtttaaaa ttacgtgaat ccattattaa tgatataggt attcgagaat acggatatac
     1801 agccaatgga tatactatgc tgatgaataa tgaacaatat actaaactta aaaaaatatc
     1861 tattatttct gaaatagtaa aagcagaaat gcgcgaagga caatggtacc gtccatgctt
     1921 tccacaacat ataaactatc cgtggaacaa agatttcttt ggacctcttg taataccaaa
     1981 agcaggaact acaattcaat taaccacaga aaatttaccg ttgtatgaac gtattattga
     2041 tgtatatgaa ggtaatgatt tacgtgtaga tggtattgac atatatataa ataatgtaaa
     2101 aacttcgacc tatacattca aaatgaatta ttactggatg atgggcgaca atagagataa
     2161 ttcccaagat tcacgattct ggggttttgt tcccgaagac catattgttg gtaaagcatc
     2221 acttatttgg ctatcgttga acgacaaagg tgtattccct ttcaatatta gatttaacag
     2281 attgtttacg gtaatacata aaaactaaac aaacactatg cttttgtcta cagcatacat
     2341 gccatgtata gaatacatgg catatatagc tcaatctgat tgtgtaacca ttgaagctca
     2401 cgagcattat atcaaacaat cgtaccgtaa tagggcctat atagccactg ccaatggcat
     2461 tatggaactt ataattccgg taattcaacc taacggcagt aaaacacata ttcgcgatgt
     2521 acagatatca tatgccgaac catggcaaaa aaaacattgg catgctatag tttcggcata
     2581 taactcatcg ccgtattttg aatattacca atatttgttt gcacctttct atactcagaa
     2641 atttaccttt ttatgggatt acaataacca atttttatca attattctca aactttttgg
     2701 aattactaca tctattctgt attccaattc ttttacccca ctacacacga tagaacacga
     2761 taaacgctat acactaaccc caaaacatat tagcacttat aaaacaccaa aatacccaca
     2821 agtatttgaa gaaaaacatg gttttgtgcc aaacttgagt tgtatagacc tattgtgcaa
     2881 tgtgggacct gaagcaaaaa aaaatgtatt atacatgggc atgtaaacag ctcatgtata
     2941 atacattata ttcaaaatat ccgtattgct ttattctaat atttaataat ataattaagt
     3001 actatatacg gttgcacaat aggtattgca gtaccacttc caccacttgt tacactgccc
     3061 gataacgtat gagtat
//
LOCUS       DAOTOW010000215         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24932_length_3075_cov_2.990397, whole genome shotgun sequence.
ACCESSION   DAOTOW010000215 DAOTOW010000000
VERSION     DAOTOW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24932_length_3075_cov_2.990397"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1024
                     /locus_tag="P5243_04815"
     CDS             149..1024
                     /locus_tag="P5243_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18804.1"
                     /translation="MKTQKLITIIVSCSVALSFQNCSKDRIETNKKSYESMNAYFDSK
                     KQEEQEFIIDTLGECPITGKLGTHICIQKSDLQFANGDSVYFPYILKLVELYSIKDMI
                     YYQATNNTTDGYFSSKGEVRIRTFKNESELQLRSNKSIEVEIKDSTSNQDLSVYYQQN
                     ATPFMWNKSNELYSDTDYGYLSLVSLGWNSAGKQIANTNTAKITFSSETDNLETVEMF
                     LYIHSGQSIVKINNHKEIQAPLGEEVTIICMAIDKQNQLYSFKTTKLISGNLAIDVSL
                     TKISDEELTQILDNL"
     gene            1229..1885
                     /locus_tag="P5243_04820"
     CDS             1229..1885
                     /locus_tag="P5243_04820"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRS18805.1"
                     /translation="MTTNTISQTQVQGTMFELPKLPYSYSALAPYIDARTMEIHYEKH
                     HGTYTNNLNAAIKGTELEGKSIEYILSTISKHSAAVRNNGGGYYNHNLFWSILSPNGS
                     EKPAENLEISKAIIKDFGSFDEFKSQFSQAATTRFGSGWAWLSVDASGKLFVSSSPNQ
                     DNPLMDVAEKKGTPILGLDVWEHAYYLNYQNRRVDYIAAFYNIINWNEVNKKYLEAKK
                     "
     gene            1969..2934
                     /locus_tag="P5243_04825"
     CDS             1969..2934
                     /locus_tag="P5243_04825"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRS18806.1"
                     /translation="MNIPDAITYINKLTREKIPYIFIFDYALTQAYVWKTNEIPKNVH
                     VSFPSFTNCNTKYDINPPILHFIPISKQEYTQSFAIVTHHLQRGNSFLVNLTAETEIE
                     TESSLYDIFNRSAAPFKLCIDNEFVVFSPEPFITISNDCIQTFPMKGTIDARIENAEH
                     KILENNKEKAEHATIVDLLRNDMSIVANNVHVKKYRYTQKIQTNHIPLIQVSSCIAGI
                     IKPELEGLYGTIIHSMLPAGSICGAPKHKTLKIIQEAETHSRGFYTGVMGYCNGHILE
                     SAVMIRFIEQRNSKMYFKSGGGITILSNIDEEYNELIQKIYVPTY"
BASE COUNT         1216 a          461 c          439 g          959 t
ORIGIN      
        1 aagctaatat aagagttgga atactagtag acctctcctg caatagcgga gcagggtacc
       61 atagcggagc aagaaattca gtaaaatgtc ctcatcgtta attttactca tatgcaacaa
      121 aaatgtaata accctaaaaa ataaaactat gaaaacacaa aaacttatta ctattattgt
      181 aagttgttct gtagctttat catttcaaaa ctgttcaaaa gatagaattg aaacaaataa
      241 aaaatcatac gaatctatga atgcgtattt tgattcaaaa aaacaagaag aacaagaatt
      301 tataattgac acattgggag aatgtcctat taccggtaaa ttaggtactc atatttgcat
      361 acaaaaatct gatttacaat ttgctaatgg cgacagcgtg tattttccat acattctaaa
      421 attagtagaa ttatacagca taaaagatat gatatactat caagcaacta acaatactac
      481 agatggctat ttttcgagta aaggtgaagt tagaattcgt acgtttaaaa atgaatccga
      541 attacaatta cgttcaaata aaagtattga agtcgaaata aaagattcta catcaaatca
      601 agatttgtct gtatattatc aacaaaatgc tactccattc atgtggaata agtcaaatga
      661 attatattct gacactgatt atggatattt aagtttagta tcattagggt ggaatagtgc
      721 cggtaaacaa atagcaaata ctaatacagc aaaaattacc ttttcttctg aaactgataa
      781 cttagaaact gtagaaatgt ttctgtatat tcattccggt caaagtattg taaaaataaa
      841 taaccacaaa gaaattcaag ccccattagg agaagaagtt acaatcatat gcatggctat
      901 tgataaacaa aaccaattat acagttttaa aacaactaaa ttaatttccg gcaatttagc
      961 aatagatgtt tctttaacta aaatatcaga tgaagaacta acacaaatac tggataattt
     1021 ataaaaaata tttacatatt acatgttata atgcctcgta ataaaaaatt acgaggcttt
     1081 tttttgtaag aaaaaaatat taagaaacga cttacaatat aaattctatt taataatgaa
     1141 aacaatacta ttttctacag caattatact tagctttttt agtaaagctc aattaatatc
     1201 ttttactcat tattcaaaac aaattacaat gacaacaaat acaatttctc aaacacaagt
     1261 acaaggtact atgtttgaat taccaaaatt accctatagc tattctgcat tagcaccgta
     1321 tattgatgct cgcaccatgg aaattcatta tgaaaaacat catggaacat acaccaacaa
     1381 cttaaatgca gctataaaag ggaccgaact cgaaggtaaa agcattgaat acattctttc
     1441 tactatttct aaacattctg cagctgttcg taataatggc ggaggttatt acaatcataa
     1501 ccttttttgg tcaatattat cacccaatgg aagcgaaaaa cctgcagaaa atcttgaaat
     1561 aagcaaagct atcataaaag attttggtag ttttgatgaa tttaaatctc aattttcaca
     1621 agcagcaacc accagatttg gttctggttg ggcatggtta agcgttgatg cttcaggtaa
     1681 attatttgta agttcatcac caaatcaaga taacccttta atggacgtgg cagaaaaaaa
     1741 aggtactcca attttaggtt tagatgtttg ggaacatgcc tattatctga attatcaaaa
     1801 cagacgagtt gactacatag cagcatttta caacattata aattggaacg aagtaaacaa
     1861 aaaatatctt gaagcaaaaa aatagttttt gatttttcca ttcttttagt ttgaaaagca
     1921 ttcgattttg catatttcat agtatgtttg tatcggatgc ttttttttat gaatatacct
     1981 gatgcaataa catacattaa caaattaaca cgggagaaga ttccgtatat tttcatattt
     2041 gattatgctt taacacaagc atatgtatgg aaaacgaatg aaatcccaaa gaatgtacac
     2101 gtatcatttc cttcttttac aaattgcaat accaagtatg atataaatcc accaatttta
     2161 cattttatac ctatttcaaa acaagaatat actcaatcat ttgcaattgt tacacatcat
     2221 ttacaacgag gaaattcatt cttggtaaac cttacagcag aaacagaaat tgaaacagag
     2281 agttcattat atgatatttt taatagaagt gcagctccat ttaaattatg tattgataat
     2341 gaatttgtag tattttctcc ggaaccattt ataaccatat ctaatgattg cattcaaaca
     2401 tttccaatga aaggaacaat tgatgcacga atagaaaatg cagaacataa aattttagaa
     2461 aataataaag aaaaagcaga gcatgctact attgtagatt tattaagaaa tgatatgagt
     2521 atagtagcaa ataatgtaca cgtaaaaaaa tatagatata ctcaaaaaat tcaaaccaat
     2581 catattcctc ttattcaagt aagttcatgc atagcaggaa taataaagcc ggaattagaa
     2641 gggttatatg gcaccattat tcatagtatg ttaccggcag gttcaatttg tggtgctccc
     2701 aaacataaaa ctctcaaaat aattcaagaa gcagaaactc actcacgagg tttttataca
     2761 ggtgttatgg gatattgtaa tgggcatatt ttagagagtg ctgttatgat tcgatttata
     2821 gaacaacgca attcaaaaat gtatttcaaa agcggcggcg gaattactat actaagtaat
     2881 attgatgaag aatataatga attaatacaa aaaatttatg tgccaactta ttgaaactat
     2941 tcaaattcaa aatgggattc caattaatat ttcataccat caaaaacgta tggataaaag
     3001 catatatgaa ctatttaata ctaaaaatac tatttcatta gtagatgttg taaatgttcc
     3061 cccacatgca aaaaa
//
LOCUS       DAOTOW010000216         3069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_24995_length_3069_cov_2.847047, whole genome shotgun sequence.
ACCESSION   DAOTOW010000216 DAOTOW010000000
VERSION     DAOTOW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24995_length_3069_cov_2.847047"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..2799)
                     /gene="uvrA"
                     /locus_tag="P5243_04830"
     CDS             complement(19..2799)
                     /gene="uvrA"
                     /locus_tag="P5243_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRS18807.1"
                     /translation="MTQSKHIEIQGASVHNLKHVSLKIQRNSFVVITGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGKIHKPEVDYITGIPPAIAIEQKVNTANPRSTVG
                     TSTEIYDYIKLLYARIGKTYSPVSGVEVKRHSVQDVVDFIVSKPNHKIYITSPVECEK
                     SELQKKLELFLQQGFSRILCKQSIVRIEEFVQKKSNCTPQDICLLIDRIVSDDSQLSR
                     MADSIQTAFFEGNGTCIVWVEEDNAMQSYSFSNTFEADGIIFEPPMVHTFSFNSPLGA
                     CPTCEGFGKVMGIDPNLVIPNKSLSIWDDAVVCWKGDKMSEWKDELVMNAEKFNFPIH
                     KPYSELSKEQQKLVWTGNQYFGGINAFFKMVEENLYKIQYRVMLSRYRGKTICPTCNG
                     SRLKPEASYIRIQNTPITELVDLSIRDLRTFFDKLTLNEHEQTIAKRILIEIRTRLEF
                     LENVGLGYLTLNRLSNTLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPRDTHLL
                     IQVLHKLQQIGNTVLVVEHDEEIMRAAHHIIDIGPGAGSNGGEVVFEGSIDELINSTN
                     SLTADYLTGRKQIVVPQKRNAWKHAITVQGARENNLKNITVQFPIGVFTAVTGVSGSG
                     KSSLVNKILYPAVQRAIGEHGIQMGEFDGITGVQGHIFSVEFVDQNPIGRSTRSNPVT
                     YSKAFDEIRKLFADQKASQLMGFKPSHFSFNTEGGRCDECQGEGIIKVEMQFMADLEL
                     VCEECNGKRFKDEVLEVQYRKKSIYDILEMTVDEAILFFGEEKNNTTQKIVEKLLPLQ
                     SVGLGYVKLGQSSSTLSGGESQRIKLASFIGKESKEQPCLFIFDEPTTGLHFHDIQKL
                     LTSFEKLIQKGHTIVVIEHNLDVIKCADWIIDIGPEGGEHGGNIVFEGTPEQIITCKE
                     SYTGTFLKEKLK"
BASE COUNT          899 a          585 c          526 g         1059 t
ORIGIN      
        1 tatatcggta atctgcattt atttcaactt ttcttttaag aatgttccag tgtacgattc
       61 cttacatgta ataatttgct cgggggttcc ctcaaataca atgtttcctc catgttcacc
      121 accttcggga ccaatgtcaa taatccaatc ggcacatttt attacatcaa gattatgttc
      181 tataactacg atagtatgcc ccttttgaat taatttttca aacgaggtta atagtttttg
      241 aatatcatga aaatgcaagc cggtagtggg ttcatcgaat ataaataaac atggctgctc
      301 tttggattct ttgcctataa acgatgctaa ttttatacgt tgagattcgc caccgctcag
      361 agtgcttgat gattgaccaa gttttacata tcctaaacca acactttgga gtggaagtaa
      421 tttttctact attttttgtg ttgtattatt tttttcttct ccaaaaaaca gaatagcttc
      481 gtctacggtc atttctaaaa tatcataaat tgatttttta cgatattgta cttctaaaac
      541 ttcgtctttg aatcgtttgc cattgcattc ttcgcatacc aattccaaat cggccataaa
      601 ttgcatttct acttttatga taccttcacc ttggcattca tcgcatcttc caccttcggt
      661 attgaatgaa aaatgtgaag gcttgaaacc cataagttgc gaagcttttt ggtcggcaaa
      721 caattttctg atctcatcaa atgccttgga ataggtaacc gggttagaac gagtagagcg
      781 acctatcgga ttttggtcaa caaattctac cgaaaatatg tgaccttgca caccggtaat
      841 tccatcgaat tcgcccattt gtatgccatg ttctcctata gctcgctgca cggcggggta
      901 tagtattttg tttactaacg atgattttcc cgaaccactt acgcctgtta cggcagtaaa
      961 tactcctata ggaaactgta cggttatatt ttttaaatta ttttcgcggg ctccttgtac
     1021 agtaattgca tgtttccatg cattgcgttt ttgtggaaca actatttgtt ttctaccggt
     1081 caaataatcg gcagtaagcg aattggtaga attaattaat tcgtcaatac ttccttcaaa
     1141 tacaacttcg ccaccattgc ttccggctcc cggaccaata tcgataatgt gatgtgcagc
     1201 acgcattatt tcttcgtcgt gttctaccac aagcacggta tttcctattt gttgtaattt
     1261 atgtagtacc tgaatcaata aatgagtatc gcgtggatgc aaacctatac tgggttcgtc
     1321 taaaatatag agcgaaccga ctaaactact gccaagcgat gttgctaaat ttattcgttg
     1381 tgattcgcca cccgataaag tattcgataa tcggtttagg gtaagatagc ctaaaccaac
     1441 attttcaagg aattctaatc gggtacgaat ttcaataagt attcgttttg ctattgtttg
     1501 ttcgtgttca ttgagcgtaa gtttgtcaaa aaatgtgcgt aaatcacgaa tcgacaaatc
     1561 tactaattcg gtaattggtg tattttgaat gcgaatatat gaagcttcgg gttttaatcg
     1621 actgccgttg catgtaggac aaatagtttt acctctatat cgcgaaagca ttactcggta
     1681 ttgaattttg tacaaatttt cttctaccat tttgaaaaaa gcattaatgc ctccaaagta
     1741 ttgattgcct gtccacacca atttttgctg ctctttgctt aactcactat atggtttatg
     1801 tatgggaaaa ttgaattttt cggcattcat aaccaattcg tctttccatt cactcatttt
     1861 gtcgcctttc caacatacta cggcatcgtc ccatattgat aagcttttgt tgggtataac
     1921 caaattaggg tcaattccca taactttacc aaacccctca cacgttgggc aagctcccaa
     1981 ggggctattg aaactgaaag tgtgaaccat tggtggttca aatataattc cgtcggcttc
     2041 aaatgtgtta gaaaatgaat atgattgcat ggcattatct tcttcgaccc atacaatgca
     2101 agtaccattg ccttcgaaaa aagcagtttg aatagaatct gccatgcgcg acaattgtga
     2161 atcatcgctc actatacggt ctatgagtaa acagatatct tgcggagtac aattactttt
     2221 tttctgtaca aattcctcaa tacgaactat tgattgttta cacagtattc gactaaaccc
     2281 ttgttgtaaa aacaattcaa gttttttttg taattcagat ttttcgcatt ctaccggtga
     2341 agtaatatat attttatggt taggttttga tactataaaa tctaccacat cttgtacaga
     2401 atgacgctta acttcaacac cggaaacagg agaatatgtt ttgcctatac gagcatacaa
     2461 taatttaatg taatcataaa tttcggtact ggttcctact gtagaacgag gatttgcggt
     2521 atttaccttt tgttctatag ctatagcggg tggaattcct gtaatgtaat caacttcggg
     2581 tttatgaatt tttcctaaaa attggcgagc atatgccgaa agactttcaa catagcggcg
     2641 ttgcccctct gcatatattg tatcaaacgc aagtgacgat tttccggaac ccgataagcc
     2701 tgtaattacc acaaaactat tgcgttgaat tttgagtgat acatgtttta gattatgtac
     2761 cgaagctccc tgaatttcaa tatgttttga ttgcgttatt cgttgttcgc ccattactgt
     2821 tattttttac aaaaatgaga aaaaaagtga ttgtatatca ctgttgtccg aattaaaatt
     2881 tgataattat cggaaatgct attattaaag tgattaaaca aagtatgaaa aagattatgc
     2941 ttcatttttt gtgtattttc ttgttacttt ttcaacagca aaaaaagtaa ccaaccgagg
     3001 aggaacgacg agctcatgca cacaaataaa attatcatta ttgtccgata aaactacaaa
     3061 attaattta
//
LOCUS       DAOTOW010000217         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25080_length_3061_cov_2.780772, whole genome shotgun sequence.
ACCESSION   DAOTOW010000217 DAOTOW010000000
VERSION     DAOTOW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25080_length_3061_cov_2.780772"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="P5243_04835"
     CDS             <1..309
                     /locus_tag="P5243_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18808.1"
                     /translation="DFNVVVKRSKIGVCPMFHYGKSAHKDMYSGFNIGVLHNKATLNT
                     EFAGVITKDLFSFNMGARPSMQLLLYGIRYYINKNVGFNFEVGLGAPFFCNTGLQIRL
                     "
     gene            complement(590..666)
                     /locus_tag="P5243_04840"
     tRNA            complement(590..666)
                     /locus_tag="P5243_04840"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(630..632),aa:Tyr,seq:gta)
     gene            complement(948..1021)
                     /locus_tag="P5243_04845"
     tRNA            complement(948..1021)
                     /locus_tag="P5243_04845"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(985..987),aa:Asn,seq:gtt)
     gene            complement(1057..1131)
                     /locus_tag="P5243_04850"
     tRNA            complement(1057..1131)
                     /locus_tag="P5243_04850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1094..1096),aa:Val,seq:tac)
     gene            complement(1189..2508)
                     /locus_tag="P5243_04855"
     CDS             complement(1189..2508)
                     /locus_tag="P5243_04855"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRS18809.1"
                     /translation="MNFSATLDYIYNKLPCYQRIGQAAYKENLDNIIALLNYIGNPHK
                     NVRCVHIAGTNGKGSVSHMCASIFQEAGYKTGLYTSPHLIDFRERIQINGIPISKRAV
                     SSFIEKYKNFIENIQPSFFEITVALCFDYFASHNVDIAIIETGLGGRLDSTNIIVPVL
                     SIITNISFDHTQLLGNTLEKIAIEKAGIIKPNIPIIIGEYQKDIEHVFIKKAQQNNST
                     LLYAEKICTLHSKNESTWYSHFSISIEDTILYKNLQSPLTASYQKKNIQTVCAAIHNL
                     RNSFSKIHKKHIVKGIANTIQNTNLQGRWQIIQSKPLIVCDTAHNYAGIAETMKQLLS
                     LNYSDIHIIWGMVNDKDVESIMNLLPHNAHYYITKPSIERGMDVQKLADFFKNNSLSY
                     TTYHSVKKAYQTAVQRITKKSVLYIGGSTFVVADFILYLSKKERKKN"
     gene            complement(2740..>3061)
                     /locus_tag="P5243_04860"
     CDS             complement(2740..>3061)
                     /locus_tag="P5243_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18810.1"
                     /translation="KTYVAEVKSGEQTATVYYAQTRRQILEYAIATKYDGYLLIDMHH
                     KKIHEIQFPLEKQYFIPTYIKIGVILIASTILLFTLMLQEYYIYGNIGIVIFCIILYN
                     KMKK"
BASE COUNT          977 a          449 c          470 g         1165 t
ORIGIN      
        1 gattttaatg ttgtagtaaa gcgttctaaa attggtgtat gccccatgtt tcattatggt
       61 aaatcggctc ataaagatat gtattccgga tttaacatag gagtattaca taataaagca
      121 actttgaata ctgaatttgc aggagtaatt acaaaagatt tgttcagttt taatatgggg
      181 gcacgtcctt caatgcaact attgttgtat ggtatacgtt actacatcaa taaaaatgta
      241 gggtttaatt ttgaagtagg tttaggagct cctttttttt gtaataccgg cttacagatt
      301 cgattataga attagtacat gctctaatta atcatttcgc tcccttgtta cttaagtata
      361 cttaactttt ttaataatcg aagtacacat gtaactgagg aatcttttaa tgcattaata
      421 caagttttac aattcctttt cgaggagtag agatattaat ttcttccttt ttagattagc
      481 taaaatttat gcttaaaata tcaaatccac aaattttcaa cttgattcca aaatacataa
      541 tagtaattaa aaaagtgttg caaaaaaaaa ggagcctctt cgactccttt gctcctcaaa
      601 ctggacttga accagtgacc ctcccgattt acaatcggga tgctctaact aattgaacgt
      661 ataagctctt cgatatacgt tttactcttc ttgcctttta taaacatttc tctgccatat
      721 gcactttttc tatctggaaa ttcttcggta tatactacta tccaaggtat gccactctta
      781 gtaaaatttg aatgcccacg gttatgacgg tccagtcgct gttggacatt ataactgctt
      841 cctatataat atttgtctaa cgttgcacta tataaaatat atacaaaata cataatagta
      901 attaaaaaag tgttgcaaaa aaaaaaggag cctcttcgac tcctttgctc ctcaaactgg
      961 acttgaacca gtgaccctct gattaacagt cagatgctct aaccaactga gctattgagg
     1021 aatataaatt gtaaagccaa ctattagttg gctttgtggg cgaagaggga ttcgaacccc
     1081 cgaccctctg ggtgtaaacc agatgctctg aaccagctga gctattcgcc cgtgcgtttt
     1141 taaaatgcga tgcaaagata cttgtttttt aatttcgtgc aaattttttt aatttttttt
     1201 tctttctttt tttgaaagat acaaaataaa atcggcaacc acaaatgtac tgccacctat
     1261 atataaaaca cttttttttg taatacgctg aacagctgtt tgatatgctt tttttacaga
     1321 atggtatgtg gtatatgaca aagagttatt tttaaaaaaa tcagctaatt tttgtacatc
     1381 catccctcgt tctatagatg gttttgtaat ataataatga gcgttgtgag gcaatagatt
     1441 cataatagat tctacatctt tatcatttac cattccccat attatatgaa tatcggaata
     1501 atttagtgag agtaattgtt tcattgtttc tgcaatacca gcataattat gagccgtatc
     1561 gcaaacaatt aatggttttg attggataat ttgccatctg ccttgtaagt tggtgttttg
     1621 tatagtgttg gcaatgcctt tgacaatgtg ttttttatga atttttgaaa aggaatttct
     1681 gagattatga atagcagcac atactgtttg tatgtttttt ttttgatatg aagcagtcaa
     1741 tggtgattgt aaatttttgt atagtattgt atcttctata gaaattgaaa aatgtgaata
     1801 ccatgttgac tcattttttg aatgtagtgt acatattttt tcagcgtaga gtaaagttga
     1861 attattttgt tgagcttttt ttataaatac atgttcaata tctttttgat attctccaat
     1921 tataataggt atgtttggtt ttatgatacc tgctttttca atggcaattt tctctaatgt
     1981 atttcctaat aactgagtat gatcaaaact tatattggta ataattgaaa gaacaggaac
     2041 aataatgttt gttgaatcaa gtcttccacc taatcctgtt tcaataattg caatatctac
     2101 attatgcgag gcaaaataat caaagcacag agctacagta atttcaaaaa atgaaggctg
     2161 tatattctca ataaaatttt tgtatttctc tataaaagaa cttactgcac gtttagaaat
     2221 aggaatacca tttatttgaa ttcgttctct aaagtctata agatgaggtg atgtatatag
     2281 gcctgttttg taacccgctt cttgaaatat tgatgcacac atgtgcgaaa cagaaccttt
     2341 gccattagta ccggcaatat gaacacaacg tacattttta tgaggatttc caatataatt
     2401 gagtaaagcg attatattat caagattttc tttataagca gcttgtccta ttctttgata
     2461 acaaggtaat ttattatata tataatctaa agttgctgaa aagttcattt tttttatttc
     2521 aaatgtaggg aaattgcatg tattattgtc aaagaataaa aacataatat ggaattttta
     2581 tcaaacatat tactgctatt tcaagaattt tcaatcttgt tttggttttt tactttgtat
     2641 atcgtattaa tttccttttt tttgtatggt attatttcaa aaaaaagtta attgtttata
     2701 aaataataca agtaaaatgt catgcatctt ttgataattt tattttttca ttttattgta
     2761 taaaataata caaaatatta caattccaat atttccatat atataatatt cttgtaacat
     2821 caaggtgaaa agtaaaatag tgcttgcaat cagaataact ccaattttta tatacgtagg
     2881 aataaaatat tgtttttcta aagggaattg tatttcatga atttttttat gatgcatgtc
     2941 aattaacaaa tatccatcat attttgtggc tattgcgtat tctagtattt gtctgcgtgt
     3001 ttgggcataa tacactgtag ctgtttgttc tcctgatttt acttctgcaa catatgtttt
     3061 a
//
LOCUS       DAOTOW010000218         3059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25105_length_3059_cov_3.286285, whole genome shotgun sequence.
ACCESSION   DAOTOW010000218 DAOTOW010000000
VERSION     DAOTOW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25105_length_3059_cov_3.286285"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="P5243_04865"
     CDS             complement(<1..869)
                     /locus_tag="P5243_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRS18811.1"
                     /translation="MSLTVMLGIQTGFSQDVHFSQFDAAPLYFNPALSGLMECNNRFI
                     SNFKGQWSTYNSFMLSYDRPLPAFSLAGGDFGLGALINADYAGETTYGNTMLKIMPAY
                     HKEIIPGFFKLSTGLDVMFNHNSVDETKVVLPGQIDPNTGEAVVGTDFEKTSKLYSDL
                     GLGINGEFRLQNDIPINAGITFHRLLGSGGGGFATTQAPSNYRRYSLNANAVYPINTT
                     VSLLPSFIFLKQKKYNEMNAGTFVKFSLGEQTSLLDALYAGAWYRFGDALIFGLAFDK
                     PINTKWTLNFGFS"
     gene            complement(989..3028)
                     /gene="metG"
                     /locus_tag="P5243_04870"
     CDS             complement(989..3028)
                     /gene="metG"
                     /locus_tag="P5243_04870"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRS18812.1"
                     /translation="MKEFKRHLVTAALPYANGPVHIGHLAGVYIPADIYVRYLRMNQQ
                     DVLFVCGSDEHGVPITIKAKKEGCTPQDIVNRYHQIIKQSFADFGIEFSNYSRTSNAL
                     HHNVSSGFFKTLYEKGEFIEQESEQYFDAQANQFLADRYITGTCPKCSSEGAYGDQCE
                     KCGSSLSPLELINPISTITGQKPVLKKTKHWYLPLDKHEQFLREWILQGHTEWKSNVY
                     GQCKSWIDGGLQPRAVSRDLDWGVPVPVEGAEGKVLYVWFDAPIGYITSTQECTKDWE
                     KYWKSEDCKLVHFIGKDNIVFHCIVFPAMLKAHGDYILPENVPANEFLNLENDKISTS
                     RNWAVWLHEYLTDFKDQQDTLRYVLTANAPETKDNDFTWKDFQARNNNELVAILGNFV
                     NRVVVLTHKYFQGTVPAKTTLLGIDTDMLAEISTAKQAVAKNIENYKFREALKDAMQL
                     ARAGNKYLTETEPWKVIKTDEQRVATILHVCLQITANLGIIIEPFLPFTAKKIQTMFG
                     MNACTWNQSGNVELLQTGTPIGEAALLFRKIEDSEIDAQIEKLQQTKQAMNTNVNKTD
                     VSPQKPLIQYDDFSKMDIRVGTIQAAERVPKTDKLMKLTIDTGIDIRTVVSGIAHCFN
                     PEEIIGKQVSILVNLQPRMLKNIESQGMILMAQNSDGKLEFISPVSKVDNGSAIC"
BASE COUNT          875 a          533 c          530 g         1121 t
ORIGIN      
        1 tagctaaaac caaaatttaa tgtccatttg gtattgatag gtttatcaaa tgccaatcca
       61 aatatgagtg catcgccaaa acggtaccaa gctcctgcgt aaagtgcatc gagtaatgaa
      121 gtttgttctc ctaatgaaaa tttcacgaat gttcctgcat tcatttcatt gtattttttc
      181 tgttttaaga aaataaaact tggtagtaat gaaacggttg tgtttatagg atatacggca
      241 ttagcattta gtgagtatct gcggtaatta cttggagctt gcgtggtagc aaaaccaccg
      301 ccgcccgaac ctaataaacg atgaaaggta attcctgcat ttattggtat gtcgttttgt
      361 aaacgaaatt ctccatttat accaagtcct aaatctgaat atagttttga agttttttca
      421 aaatcggtac caactactgc ttctcctgta ttagggtcaa tttgccccgg taatactact
      481 tttgtttcgt ctacactatt gtggttaaac attacgtcta atccggtaga taatttaaaa
      541 aatccgggaa taatttcttt atggtatgcc ggcattattt ttaacatggt attaccatag
      601 gtggtttcgc ctgcatagtc agcattgatt aatgctccta atccaaagtc gcctcctgcc
      661 aaagaaaaag ccggtagtgg tcggtcgtat gacaacataa atgaattata tgtactccat
      721 tgtcctttga aatttgaaat aaatcggtta ttacattcca ttaatcctga aagtgcaggg
      781 ttaaaataca atggggcagc atcaaactgc gaaaaatgaa cgtcttgtga aaaaccggtt
      841 tgaatgccca gcattactgt aaggctcaaa cttagtacta ttttgtgtag ggcactactc
      901 ataaagtatg acatttttta aggcttgaat acaaaatata caaatataag tttttttttg
      961 attatcaaat atataaaata atattttgtt aacaaattgc actaccgttg tctacttttg
     1021 atacaggtga aataaattct aattttccat cggaattttg ggccattaaa atcattcctt
     1081 gtgattcaat attttttagc attcggggtt gtaaatttac taaaatactt acttgttttc
     1141 cgatgatttc ttcggggtta aaacaatgag caatacccga tactactgta cgtatatcta
     1201 taccggtatc tatggttaat ttcatgagtt tgtctgtttt tggaactcgt tctgcggctt
     1261 gtattgttcc tacgcgtata tccattttac tgaaatcatc atattgtatc aacggttttt
     1321 gcggacttac atcagttttg tttacattag tattcattgc ctgtttagtt tgttgtaatt
     1381 tttcaatttg tgcatctatt tcactgtctt ctatttttct aaacaataaa gcagcttctc
     1441 ctattggtgt acctgtttgt aataattcta catttcctga ttgattccat gtacacgcat
     1501 tcatgccaaa catagtttgt attttttttg ctgtaaatgg taaaaaaggt tcaattataa
     1561 ttcccagatt tgcagtaatt tgtaaacaaa catgaagtat ggttgctact cgttgttcat
     1621 cggtttttat tactttccaa ggttcggttt cggttaagta tttgttaccg gctcgtgcca
     1681 attgcatagc atctttcaat gcttctctaa atttataatt ttcaatgttt tttgctactg
     1741 cttgttttgc tgtagaaatt tctgcaagca tatcggtatc tattcccaag agagttgttt
     1801 ttgcaggcac ggttccttga aaatatttgt gtgtaagaac aactacgcgg tttacaaagt
     1861 ttcctaaaat agcaactaat tcattattgt tgcgtgcctg aaaatctttc catgtaaagt
     1921 cattgtcttt ggtttcggga gcattagccg taagtacata tcgtaatgta tcttgttggt
     1981 ctttaaagtc agttaagtat tcatgcagcc atacagccca atttcgtgaa gtagaaattt
     2041 tgtcattttc taaatttaag aattcgttag caggtacatt ttcgggtaaa atgtaatctc
     2101 catgagcttt gagcatagca ggaaatacta tacagtgaaa tactatattg tctttaccaa
     2161 taaaatgaac aagtttgcag tcttctgatt tccaatattt ttcccaatct ttggtgcatt
     2221 cttgtgtaga agtaatatag cctataggag cgtcgaacca tacatatagt actttgcctt
     2281 ctgctccttc aaccggtaca ggtacacccc aatctaaatc acgacttacg gcacgtggtt
     2341 gtaatccacc gtctatccat gatttacatt gtccatatac gttagatttc cattcggtat
     2401 gtccttgtaa aatccattct cttaaaaatt gttcgtgttt atccagaggt aaataccaat
     2461 gtttggtttt tttgagcaca ggtttttggc ctgtaattgt tgaaattggg tttataagtt
     2521 caagtggact tagtgatgaa ccgcattttt cacattgatc gccataggct ccttcactac
     2581 tgcatttggg gcaagtgccg gttatgtatc tgtcagccaa aaattgattt gcttgtgcat
     2641 caaagtattg ttcactttct tgttctataa attcaccttt ttcatacagt gttttaaaaa
     2701 aaccagatga tacgttatga tgcaatgcgt ttgatgtacg agaataattt gaaaattcta
     2761 taccaaaatc agcgaatgat tgtttaataa tttgatgata tctgtttaca atgtcttgag
     2821 gtgtacatcc ttcttttttt gctttaattg ttataggtac accatgttcg tcacttccgc
     2881 acacaaataa tacatcttgt tggttcattc gtaaatagcg cacatatata tcggcaggaa
     2941 tgtatactcc tgctaaatga ccaatatgaa caggaccatt ggcatatggt aatgctgcag
     3001 ttacaagatg tcgtttaaat tctttcattc cggtaaattt gtttaattcc atgtaacat
//
LOCUS       DAOTOW010000219         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25201_length_3051_cov_2.543057, whole genome shotgun sequence.
ACCESSION   DAOTOW010000219 DAOTOW010000000
VERSION     DAOTOW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25201_length_3051_cov_2.543057"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..2761)
                     /locus_tag="P5243_04875"
     CDS             complement(149..2761)
                     /locus_tag="P5243_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS18813.1"
                     /translation="MYITYGNGVGPHGHWDPASETMTEGAVWKYVPTGNVWTNITPGV
                     ASAYGGVSVDPTNANRVIVSTINKYLQQPWGWGDRIFVSTNGGSAWTDLIGSNKVAMS
                     NNGIPWIDGHAIHWAGCVTIDPFNTERVFVTSGNGIFMTENISAATSTWSFMAKGIEE
                     TVPLDIVSCINGPVVTVIGDYDGSVYSDLETYPTLHNPQIGTTTGIDVSADNSLMVRV
                     GGATDGSNFPIYYSTNKGSTWTAFSSKPNNSNFYKGKAAVSSNGATVIWAPENSSTLY
                     KTTNRGGSWTTVSGISLTNAYPVADGVNANKFYIANGNTIYVSTNAGTSFSAAGTTAN
                     TDLKKIRAVPGVEGDIWAPTGTNGLYRSTNSGTSFTKINTVTACSAIGFGKAAPSKTF
                     PTVYIWGTVSGVTGIFRSIDAGATWVRINDDAHEYGGTANGQFVIGDMNTYGTVYMST
                     AGRGTVVGTVSVSDTQAPTTPGTITFSAITQNSFTASWTASTDNVGVVGYEVLLNGTL
                     YATVTTNSISVTGLTCNTSYSVRVRAKDAAGNFSAYNTQVSTTTTGVAAPTITNTTPT
                     ICAGGTISLSIPTTTGATYAWTGPSSYSATTAAISRTNATTAMGGTYSATVTVNGCTS
                     TASSTTVTVNAIPVAPTVTSPIVYEQGTSAIPLTASGNSLLWYTVATGGNASSSAPTP
                     STALIGTTIYYVSQTINSCESPRALVIVQVVQPPITQTIELQSGWNLISIQVQPSDSS
                     FETIFNGLQVQTVKNAQGFWRKDNPTQLNSIHKIMPCQGYLVYMTAPGTLQIIGKSCN
                     SDLFISPSSGWQLIGYPCIGLSIFSNIPFQSSLEISNNCQIIKNFDGFWEPNGSSNSI
                     QNFEQGKGYYVLKK"
     gene            complement(2742..>3051)
                     /locus_tag="P5243_04880"
     CDS             complement(2742..>3051)
                     /locus_tag="P5243_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18814.1"
                     /translation="FGGTNGYNCFHPDSIKENLVVPSVAIVDFKLFNKSVGVGELVRG
                     KAILKKNILETDKIVLSYMDNVISFEFAALQYNSPEFNQFTYILEGYEKEWNNVYNIW
                     "
BASE COUNT          958 a          602 c          510 g          981 t
ORIGIN      
        1 tatattaagt ataaccatta tacttaataa aaaactttat aaatctacaa gcaaaagtta
       61 atcaaaataa acaattgaac tcaaatcatg agctatgtta tgtttcttac ataaacaatt
      121 aatataagaa attctataac aattatttct attttttaag aacataatac ccttttcctt
      181 gctcaaagtt ttgaatagaa tttgaactac cgtttggctc ccaaaatcca tcaaaatttt
      241 taatgatttg acaattatta gaaatttcta aactactttg aaatggaata tttgaaaaaa
      301 ttgataaccc tatacatgga tatcctataa gttgccaacc agatgaaggt gaaataaata
      361 aatctgagtt acaagacttt cctattatct gtaatgttcc cggagctgtc atatatacca
      421 aatagccttg acaaggcata attttatgga ttgaattaag ctgtgttgga ttatcttttc
      481 tccaaaatcc ttgtgcattt tttactgttt gaacttgcaa accattaaaa atggtttcaa
      541 atgaactatc tgagggttgt acttgtattg aaattaaatt ccaaccactc tgtaattcta
      601 ttgtttgtgt tataggcggt tgcacaactt gaacaataac caaagccctt ggactttcac
      661 aagaatttat tgtttgcgat acataataaa tagtagttcc tattaatgct gttgaaggtg
      721 ttggagccga agagctagca ttaccacctg ttgctactgt ataccacaac aatgagttac
      781 ctgaagctgt aagaggtata gcagatgttc cttgttcata aacaattgga cttgtaacag
      841 taggagcaac agggattgca ttcactgtaa ctgtagtaga acttgcggta cttgtacacc
      901 catttactgt tacagttgca gaatatgttc cacccattgc agttgtagca tttgttctgc
      961 taatagcagc cgtagtagca ctatagctgc ttggccctgt ccatgcatag gtagcgcccg
     1021 tagtggtagg aatgctgagc gatatagttc cacccgcaca aatggttggg gtagtattgg
     1081 taatagttgg cgcagcaacc ccggtagtgg tagttgatac ttgtgtatta tatgccgaaa
     1141 aattgccggc agcatcttta gctcttactc taacagaata tgatgtatta cacgttaatc
     1201 ctgttactga tatagaatta gtagttacgg ttgcatataa agttccattg agcaagactt
     1261 cataccccac aactccgacg ttatcagtag atgcagtcca cgatgcagta aatgaatttt
     1321 gtgtaattgc actaaaggta attgttccgg gtgtagtagg agcttgagta tccgaaacac
     1381 ttacagtgcc tacaacagta cctctacctg cagtactcat atatacagtt ccataagtat
     1441 tcatatcacc tattacgaat tgtccgttag cggtaccgcc atattcatga gcatcgtcat
     1501 ttatacggac ccatgttgcc ccggcatcaa tacttctaaa tattccggtt acaccactta
     1561 ctgtacccca aatatagact gtaggaaacg ttttagaagg agctgcttta ccaaaaccaa
     1621 tagcagaaca tgcagttaca gtatttattt ttgtaaaaga tgttccggaa tttgttgaac
     1681 ggtataatcc atttgtacct gttggagccc atatatcacc ttctacaccg ggaacagcac
     1741 gaattttctt taaatcggta ttagctgtag tgccggcagc agaaaatgaa gtacctgcat
     1801 tagtacttac atatatagtg ttgccattag ctatataaaa tttatttgca tttacaccat
     1861 cggcaaccgg atatgcatta gtaagtgaaa taccagaaac tgttgtccat gaaccacctc
     1921 tatttgttgt tttatatagt gtacttgaat tttcaggtgc ccaaattact gttgcaccat
     1981 tagacgacac cgctgcttta cctttataaa aattagaatt atttggtttt gaactaaatg
     2041 ctgtccacgt acttccttta tttgttgaat aatatattgg aaaattactt ccatctgtag
     2101 caccaccaac ccttaccata agggaattat ctgcagatac atcaatgccg gtagttgtac
     2161 caatttgagg attatgtaat gttggatatg tttctaaatc ggaatacaca gaaccatcat
     2221 aatcacctat tacagttaca accggtccat ttatacaact aacgatatca agcggaactg
     2281 tttcttcaat accttttgcc ataaaactcc aagtagaagt agctgcacta atattttctg
     2341 tcataaaaat tccattccct gatgtaacaa atactcgttc tgtattaaac ggatcaatag
     2401 tgacacaacc tgcccaatga atggcatgac catctatcca aggaataccg ttattactca
     2461 ttgctacttt attgcttcct atcaaatctg tccatgcact accaccattt gtagacacaa
     2521 aaattctatc accccaaccc catggttgtt gtaaatattt attaatagta gaaactatta
     2581 ctctattggc attagtagga tctacagaaa ctccaccata tgcagatgca acaccgggtg
     2641 taatattggt ccacacattt ccggtaggta catatttcca cacagccccc tctgtcatag
     2701 tttcactcgc gggatcccaa tgtccatgag gtccaactcc attaccatat gttatataca
     2761 ttgttccatt ctttttcata tccttccaga atgtaggtaa attggttaaa ttctggagaa
     2821 ttatattgta aagcagcaaa ttcaaaagaa attacattgt ccatatagga aaggacaatt
     2881 ttatccgttt caaggatgtt ttttttgagt atggcttttc ctctgacaag ctcgccaact
     2941 ccaacacttt tattgaatag tttaaaatca acaatcgcca cagaggggac aacaaggttt
     3001 tctttgatac tatcgggatg aaaacaattg tagccgtttg ttcctccaaa a
//
LOCUS       DAOTOW010000220         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25219_length_3049_cov_3.253507, whole genome shotgun sequence.
ACCESSION   DAOTOW010000220 DAOTOW010000000
VERSION     DAOTOW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25219_length_3049_cov_3.253507"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1348)
                     /locus_tag="P5243_04885"
     CDS             complement(182..1348)
                     /locus_tag="P5243_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18815.1"
                     /translation="MNILSNKIQNSIIILCSLFCAIIFFNLFGFLRFNEADTPNFINA
                     AKILFGATDLIDCQSRITKPFVLLSPGFLYTYFGISITVTMLIQNLIFFVGTGFFTAK
                     ILQYWGFDFTLQLLGVFIIYTVQPLAVLSFMLINDIAGYFFTTFLLYYYLQTKVQTWK
                     QCVVFSLIAIAGVLSKESSALALIVVLLYMLMYHSKQWIRFVVMYAAIIIAYMGVQWY
                     IQHTFGYVSSLENLTQKYEEPEGYIFKIQQLIHSFDVYWIYIIVGVLYIILRKSPYSF
                     FAWATVVTIPFLFLWPSVQDRTIAVAAPIFVWVIISFLQQSTWLQKGLLIVGGISNIV
                     CTYIIYAYKTSNVLPVYYTIFFVLFALLYKEKNIKKRLLVLTNNLLHWGEGENI"
     gene            complement(1360..2079)
                     /locus_tag="P5243_04890"
     CDS             complement(1360..2079)
                     /locus_tag="P5243_04890"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRS18816.1"
                     /translation="MVYSLFTQPKKQLALLIDPDKHTNESLQYIATVAQKIGVDYVFV
                     GGSLLITDISDCVCTIKSYYTGPVILFPGHANQVCSSADAILFLSLISGRNPEFLIGN
                     HVIAAPAIKQAGLEVIPTGYILIECGKTTSVEYMSNTRPIPGTKPDIAVATAMAGEML
                     GLRAMYIEGGSGADNTVNTTIIQKIKQSCTVPIIVGGGIQTPHMLTSIFEAGADIAVI
                     GTIIEQQPELLAPMMECVRRL"
     gene            complement(2083..2703)
                     /locus_tag="P5243_04895"
     CDS             complement(2083..2703)
                     /locus_tag="P5243_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRS18817.1"
                     /translation="MITNALYTVDSKEFTLSVVPLVTNVSQEHIAVLNILEIEQLQTI
                     ESSSRKAEFVTSRNVLHSILGPQYEPIQYAETGKPYMNSMNISISHTKQFVAVIVSKQ
                     YNVAVDIEMYRSKIHTIASRFMNTQEYSFYVSTEDRTVIWCAKEVLFKLYNASPNFKE
                     HYSINICDLHDSGTLTATLQTPAIEKIIELSYFRTLQFCLVWAYNS"
     gene            complement(2700..>3049)
                     /locus_tag="P5243_04900"
     CDS             complement(2700..>3049)
                     /locus_tag="P5243_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18818.1"
                     /translation="TVGYSNFYSNLVQKSIIYDFAFEYSDKRRAELSRIVNYKDFETY
                     LVSHNVWNLLLAYAQKRGVHASRTDIFVSKKRIETQLYAYIARTIFGETEFYRIVNKD
                     DTTIQQALHALKK"
BASE COUNT         1094 a          527 c          428 g         1000 t
ORIGIN      
        1 taatggcata aattctacca tttcaatata tgatatgcag ggtatattag tattacaaac
       61 tacttcttgt caagatattg aaactatatc tatgagttca ataccttcag gaatgtatat
      121 agtagaagtt tgtcaagaaa atgtaaaaaa atcatttact atactcaaac aataatttcc
      181 cctatatatt ctctccctcc ccccaatgca agaggttgtt agttaaaact aacaacctct
      241 tttttatatt tttttcttta tacaataaag caaacagcac aaaaaatata gtatagtaca
      301 caggtaacac attagaagtt ttgtaagcat aaattatata tgtacatact atattagaaa
      361 ttcctcctac aataagtaat cctttttgca accatgtaga ttgctgcaaa aaactaataa
      421 taacccaaac aaatatagga gcagctactg caatagttct atcttgaacc gaaggccata
      481 aaaaaagaaa cggtatagta actactgttg cccacgcaaa aaaagaatag ggtgatttac
      541 gtaaaataat atacaataca ccaactataa tatatatcca atacacgtca aaactatgta
      601 taagttgttg aatcttaaaa atatatcctt caggttcttc atatttttga gtaagatttt
      661 ctaaactgct tacatatccg aaagtatgtt gtatatacca ttgaactccc atatatgcaa
      721 ttataatagc ggcatacatt actacaaacc gaatccattg ttttgaatga tacatcaaca
      781 tatagagcaa aacaacaata agtgctaaag cactgctttc tttacttaat actccagcaa
      841 ttgcaattaa tgaaaataca acacattgtt tccacgtttg aactttagtt tgtaaataat
      901 aatataataa gaatgtagta aaaaaatatc ctgctatatc atttataagc ataaaactaa
      961 gtaccgcaag aggctgaaca gtataaataa taaaaacacc taataattgc aacgtaaaat
     1021 caaatcccca atattgtaaa atttttgctg taaaaaaacc cgtaccaaca aaaaaaataa
     1081 ggttttgtat aagcattgtt actgtaatag aaataccaaa ataggtatat aaaaacccgg
     1141 gggaaagtaa cacaaatggt tttgtaattc gtgattgaca atcaatcaaa tcggtagcac
     1201 caaacagtat ttttgccgca tttataaaat taggcgtatc ggcctcattg aaacgtaaaa
     1261 acccgaatag attgaaaaaa ataattgcac agaacaatga acaaagaatg ataatactat
     1321 tttgaatttt attgctcagt atattcatac aattatgtat tacaatctgc gaacacactc
     1381 catcatagga gcaagcaatt cgggctgttg ttctattata gtacctataa cagcaatatc
     1441 ggcccctgct tcaaaaattg atgtaagcat atgcggtgtt tgaataccac cacctactat
     1501 aatgggtacc gtacatgatt gtttaatttt ttgaattata gtagtattca ctgtattatc
     1561 tgctccgctg ccaccttcta tatacatagc acgcaaacct aacatttcac cggccatagc
     1621 tgtagcaacg gcaatatcag gttttgtacc cggtattgga cgagtgttgc tcatatattc
     1681 taccgatgtg gttttaccac attctataag tatatatccg gttggtataa cttctaatcc
     1741 tgcttgtttt atagcagggg ccgctattac atgattacct attaaaaact cgggatttct
     1801 ccccgaaatt aatgataaaa acaaaatagc atcggccgaa ctacatactt gatttgcatg
     1861 tcctgggaat aaaattacag gacctgtata atatgatttt atagtacaaa cacaatcgga
     1921 aatatcagta attaataaac taccacctac aaatacataa tctactccaa tcttttgggc
     1981 tactgttgca atatattgta atgattcatt cgtatgctta tcggggtcaa taagcaatgc
     2041 taattgtttt ttgggttgtg taaataaact atataccata cgttatgaat tatatgccca
     2101 caccaaacaa aattgaagtg tacgaaaata cgataattct attatttttt caatagcagg
     2161 agtttgtaaa gtagcggtaa gagttcccga atcatgtaaa tcgcatatgt taatactata
     2221 atgttcctta aaatttgggc tagcattata caatttaaac aacacttctt ttgcacacca
     2281 tattaccgtt ctatcttctg tacttacgta aaaactatat tcttgagtat tcataaaacg
     2341 cgaagcaatg gtatgaattt ttgaacgata catttcaata tcaacggcaa cattgtattg
     2401 ttttgaaaca atgacagcta caaattgttt tgtgtgactt atacttatat tcatagaatt
     2461 catataaggc ttacctgttt ctgcatattg aataggttca tactgtggcc ctaagataga
     2521 atgcaataca ttacgtgaag taacaaactc agcttttctc gaagatgatt ctatagtttg
     2581 caactgctct atttctaata tatttaatac ggcaatatgt tcttgagaaa cattagttac
     2641 taaaggaact acacttaatg taaattcttt tgaatcaacc gtatataatg cattagttat
     2701 cattttttta gtgcatgtag agcttgctga atagttgtat catctttatt tactattctg
     2761 taaaattctg tttctccaaa aatagtacgt gctatatatg catacaattg tgtttcaatt
     2821 cgtttttttg acacaaatat gtcggtacgc gatgcatgca cacctctttt ttgtgcatac
     2881 gcaagcaata aattccatac attgtgcgac acaagatatg tttcaaaatc tttgtaattt
     2941 actatgcgcg ataattcagc tcttcgttta tcactatatt caaatgcaaa atcataaatt
     3001 atgctttttt gaaccaaatt agaataaaaa tttgagtacc ctacagtat
//
LOCUS       DAOTOW010000221         3045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25254_length_3045_cov_2.936120, whole genome shotgun sequence.
ACCESSION   DAOTOW010000221 DAOTOW010000000
VERSION     DAOTOW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25254_length_3045_cov_2.936120"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..275)
                     /locus_tag="P5243_04905"
     CDS             complement(105..275)
                     /locus_tag="P5243_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS18819.1"
                     /translation="MKEHVFKNKSRNFAIRIVKLYQYLNTEKKEFVLSKQLLRSWLSV
                     TAMVHEPVSGIY"
     gene            complement(303..1565)
                     /locus_tag="P5243_04910"
     CDS             complement(303..1565)
                     /locus_tag="P5243_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18820.1"
                     /translation="MQKEKLQEILLDQRESFLRERYIINRDINLEPYISTSQVVIISG
                     VRRCGKSTLLYSIKQIMQIPNSKFCYCNFDDERIIPENDILEQIYRTHIEMFNEEPIF
                     FFDEIQTIPLWEKFVNRMHEKGNKLFVTGSNSTLLSSEIGTSLTGRNKILQLFPFSFK
                     EFCRFKQHSYNIAQLSTTEKSRLLYDLHSYMEIGGFPIVVKENDIEIIHNWFQDILYR
                     DIISRFKILQHVEIKILALFLISNTSKIFSYATLQKISHIKSLSSIKNYLHYFELSYM
                     FFYVKKFDFSVQKQISNSKKVYSIDTALCNKLGFKFSENKGRLLENMVFLELLRQKKE
                     IYYYANNKECDFIVKQGIQIEQAIQVVYELNQENYERELQGLLYAMNDLQISNGLIIT
                     FDDNTFPLNTPENVKCISIWRWFFENNY"
     gene            complement(1634..1771)
                     /locus_tag="P5243_04915"
     CDS             complement(1634..1771)
                     /locus_tag="P5243_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS18821.1"
                     /translation="MLFSIIIPTYNRAHILPHTIASVLAQTYTNWECIIVDDGSTDTT
                     K"
     gene            complement(1829..2194)
                     /locus_tag="P5243_04920"
     CDS             complement(1829..2194)
                     /locus_tag="P5243_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS18822.1"
                     /translation="MGKTLMHDKSIDFAIRIVKLSQYLCAEHKEFNLSKQLVRSGTSI
                     GAMIRESEYAESIADFIHKFSIAQKECNETMYWLELLYKTNYLTLPEYTSISQQSTEL
                     LKMLTTTIKTCKSKLPKNN"
     gene            2347..2847
                     /locus_tag="P5243_04925"
     CDS             2347..2847
                     /locus_tag="P5243_04925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS18823.1"
                     /translation="MQITQSHISVVTPVYGCKTCLVELYLRLKTTLEQITQDFEIIMV
                     NDASPDGAWETIVELAARDQRVKGINLSRNFGQHYAITAGLDHAQGEWVVVMDCDLQD
                     QPEEIVKLYAKAQEGWDIVFARRTQRQDSFTKRLFSKIFHRVFSYLSGIKQIIPLQTL
                     VFLIQK"
     gene            2865..>3045
                     /locus_tag="P5243_04930"
     CDS             2865..>3045
                     /locus_tag="P5243_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18824.1"
                     /translation="MKETARSFPSLVQYLGFSKSTVDVQHSSRFEGKTSYSFAKLIQL
                     SSDVIFSNSNKPLKLA"
BASE COUNT          988 a          424 c          493 g         1140 t
ORIGIN      
        1 agatattagg ttttagatat tagttgtttt ttggtagttt tgatttgcat gttttaattg
       61 ttattgtaat catttttaat agttctgttg attgttgcga aatactagta tattccggaa
      121 acaggttcat gcaccatagc tgtaactgat aaccaggaac gtaagagttg ttttgataaa
      181 acaaattcct tcttttcagt attcaaatat tgatataatt ttacaattcg tattgcaaag
      241 ttacgtgatt tattcttaaa aacatgttct ttcatgtatc attaataatt aactattgat
      301 aattaataat tattttcaaa aaaccaacgc catatactta tgcactttac attttcgggg
      361 gtgtttaacg gaaaggtatt gtcgtcaaaa gtaataataa gtccattcga tatctgcaaa
      421 tcattcatag catagagtaa tccttgtaat tctcgttcgt aattttcttg atttagttca
      481 tatactactt gtattgcttg ttctatttga atcccctgtt taacaataaa gtcacattct
      541 ttattatttg cataataata tatttctttt ttttgtcgca ggagttccaa aaaaaccata
      601 ttttctaata atcgcccttt attttctgaa aatttaaatc ctaatttatt gcaaagtgct
      661 gtatcaattg aatacacttt ttttgaatta cttatttgtt tttgtacaga aaaatcaaat
      721 tttttcacat aaaaaaacat ataacttaat tcaaaatagt gcaagtaatt ttttattgaa
      781 cttaaacttt ttatgtgcga tattttttgt aatgtagcat acgaaaatat tttactcgta
      841 ttagaaatta aaaataatgc taaaatttta atttcgacat gttgaagaat tttaaatcta
      901 ctaataatat ctctatatag aatatcctga aaccaattat gtatgatttc tatatcattt
      961 tctttaacaa caataggaaa tccaccaatt tccatatatg agtgtaaatc atataatagt
     1021 ctcgattttt ctgttgtaga aagttgtgca atattgtaag aatgttgttt aaatcggcaa
     1081 aattctttaa atgaaaaagg gaacagttgt aaaattttgt ttctccctgt aagcgaggtt
     1141 ccaatttcgg agcttaacag ggttgaattt gaaccggtaa caaaaagttt attgcctttt
     1201 tcgtgcattc tatttacaaa tttctcccac aatggaattg tttgaatttc atcgaaaaag
     1261 aatattggct cttcattaaa catttctata tgagtgcgat agatttgttc taatatgtca
     1321 ttttcgggaa taatccgttc gtcatcgaaa ttacaataac aaaatttcga attaggtatt
     1381 tgcattattt gtttgattga atacaataac gttgatttgc cgcatcttcg cacgccggaa
     1441 attataacaa cttgtgaagt tgatatataa ggttccaaat taatatcgcg attaatgata
     1501 tagcgttctc taagaaatga ttctctttgg tctaataaaa tttcttgtaa tttctctttt
     1561 tgcattatct ttctttgtta aagaacaaat atactaaatt ttattcgtta tcaaagaaaa
     1621 gaattaatta tttttatttt gtggtatctg tactgccatc gtctacaatt atacactccc
     1681 aattggtgta ggtttgtgcc agcacgcttg caatagtgtg ggggagaatg tgtgcacggt
     1741 tgtatgtggg gattatgatg gagaaaagca tggtagatat tagatgttag gtatgaggta
     1801 ttagatatta gatattaggt tttagatatt agttgttttt tggtagtttt gatttgcatg
     1861 ttttgatagt tgttgtgagc atttttaata gttctgttga ttgttgcgaa atactagtat
     1921 attccggtaa tgtaaggtaa tttgttttgt agagtaattc taaccaatac atagtttcat
     1981 tacattcttt ttgagcaatt gagaatttat ggataaaatc agcaatactt tcggcatatt
     2041 cactttcacg aatcattgct ccaattgaag tgccgcttcg tactaattgt tttgaaagat
     2101 taaattcttt atgttctgca caaagatatt gagaaagttt tacaattcgt attgcaaaat
     2161 ctatactttt atcatgcatt agtgttttcc ccataatttg ctgcttgttt atgtgaaaca
     2221 aaaatatgaa aaaatattag atataaggca ttagatataa agtataagat tgggaatgtt
     2281 aaatttctca cccctcatat ctaatatctc atatctaata tctaaaaatt atttttatat
     2341 ttgtacatgc aaataactca atcacatatt tcagtagtta cccctgtgta tgggtgcaaa
     2401 acgtgtttgg tagaattgta tctacgctta aaaacaacgt tagagcaaat tacacaagat
     2461 ttcgaaataa taatggtaaa cgatgccagc cctgatggag catgggaaac catagtagaa
     2521 cttgcggcac gtgaccaacg agtaaaagga atcaatttat ctcgtaattt tgggcagcat
     2581 tacgcaatta ccgcaggctt agaccacgct caaggtgaat gggtagttgt aatggattgt
     2641 gatttacaag accagcccga agaaattgtg aaattgtatg ccaaagcaca agaaggttgg
     2701 gatattgtat ttgcacggag aacacagcgg caagattctt ttacaaaacg acttttttca
     2761 aaaattttcc atcgcgtatt ttcttattta agtggaataa aacagataat accattgcaa
     2821 actttggtat ttttaataca aaagtaatcc gagaatttaa tacgatgaaa gaaacggctc
     2881 gttcatttcc gtcgttagtt cagtatttag gattttcgaa aagtacggtt gatgttcaac
     2941 actcctctcg attcgaaggt aaaacttcgt attcgtttgc aaaacttatt cagttatcga
     3001 gcgatgtaat tttttcgaat agcaataagc cattaaaatt agcgg
//
LOCUS       DAOTOW010000222         3040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25295_length_3040_cov_2.903853, whole genome shotgun sequence.
ACCESSION   DAOTOW010000222 DAOTOW010000000
VERSION     DAOTOW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25295_length_3040_cov_2.903853"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="P5243_04935"
     CDS             <1..96
                     /locus_tag="P5243_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS18825.1"
                     /translation="IADLDSSEDIMPIHVAEAIQYRSLDRENWAG"
     gene            364..897
                     /locus_tag="P5243_04940"
     CDS             364..897
                     /locus_tag="P5243_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18826.1"
                     /translation="MKKIITLLTSLCIGSLFMSSCVSTNKGFQSSPIISRNVELDPIK
                     ADIKVDEKNKLKGESTSTYFLMFRVSGDNTFADGINYSTDASAGVMQRINPLKLAQSG
                     RLNSVRGAAAYKALSSGDYDVLVHPNYTMTIENYLFVKKYTVSVEGYGAKYTNFRTEK
                     RKVVLLENGKELILQDK"
     gene            1133..>3040
                     /locus_tag="P5243_04945"
     CDS             1133..>3040
                     /locus_tag="P5243_04945"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRS18827.1"
                     /translation="MIHDFDTIQKTFVTAQQKMKRIRACTKRPLTLTEKILFTHLDDS
                     EIPQVDTIVRGKDYCNFYPDRVAMQDATAQMALLQFMNAGKQRVAVPSSVHCDHLIIA
                     EQGEVADLATANDKNAEVYNFLSSVSQKYGIGFWKPGSGIIHQIILENYAYPGGMIIG
                     TDSHTVNGGGLGMIAIGVGGADACDVMSGMAWELKMPKIIGVKLTGTCNPWVAPKDVI
                     LKLAGILTVKGATNAVLEYFGEGAQTLSCTGKATICNMGAEVGATTSIFAYDQSMKEY
                     LHHTGRKAVADLVETYSEILQADDEVLQNPHSYYDSIIEINLSELEPYINGPFTPDLA
                     TPISKFAEVARKEKWPLELKVGLIGSCTNSSYEDLTKAASLARQAQELELDIQSEFLI
                     SPGSVMIKKTAKRDGLLAAFEAIGGKVLANACGPCIGQWNRENPEPELKNSIITSFNR
                     NFAKRADGNPNTYSFVASPEIVTALTIAGRIDFNPITDSLINKHGKQVKLQAPQGNVI
                     PEKGFEMVDAGFIAPDDKNAHIEILINPQSERLQILQPFAPWNGKDFIGLQLLLKAQG
                     KCTTDHISMAGPWLRYRGHLENISDNLLMGAVNAFNGQTNSVCNALTGLYDKVSSVAK
                     AYKQKGLASIVV"
BASE COUNT         1091 a          421 c          579 g          949 t
ORIGIN      
        1 attgccgatt tagattcttc ggaagatata atgccaattc atgtagccga agctatacaa
       61 tatagaagtt tagatcgaga aaattgggca ggctaaccat tttttattta aaaattagta
      121 gttttataag tattttgttg tatatttgaa tacgcaaaag actgtatgca agcgggaaga
      181 ttttgtataa ttaactgagc ctgcttatga ttttactata ccattgtaat ttttttaata
      241 cttcataaga taagtaatgt gtatgttact attataatac tatgtggagt ctgacaccat
      301 aaaaaacggg gagtattctg aaaatcctta cgagtaagat tgattatttt aaaaattaca
      361 attatgaaaa aaattattac attattaact agtttatgta taggctcatt gtttatgagt
      421 agttgcgtgt caacaaacaa agggtttcaa tcaagtccta ttatatcacg aaatgttgaa
      481 ttagatccta taaaagctga tataaaagtt gacgaaaaaa ataaattaaa aggagaaagt
      541 acgtctacat acttccttat gtttagagta agtggagata atacttttgc tgatggcatt
      601 aattatagta ccgatgcatc ggcaggtgta atgcaaagaa taaacccact caaattggct
      661 caatcaggca gattaaattc tgtaagaggt gccgctgcat acaaagcttt gagttcagga
      721 gattatgatg ttttagtgca tcctaattat acaatgacta ttgaaaatta cttgttcgta
      781 aaaaaataca cagttagtgt ggaaggttat ggcgctaagt atacaaattt tagaacagaa
      841 aaaagaaaag ttgttttgct tgaaaatggt aaagaattga tattgcaaga taaataaaac
      901 aaatttttaa aagttgtaat taaaaaaggg ttgtttggat ttgccaaaca acccttttta
      961 tttaaaaagt atgttttaca ttagtttttg tgatagttga acccaaacaa caatgaaata
     1021 taatataaaa aatgctattg aatatcgttt tttttataat accaattaaa atggattcat
     1081 actatattta cattgaaaaa aaagttacga ttttgaatag ttataataca agatgataca
     1141 cgattttgat acaatacaaa aaacatttgt tactgcacaa cagaaaatga aacgtattcg
     1201 tgcatgtaca aaacgaccat tgacacttac cgagaaaatt ctttttactc atttagacga
     1261 ttcagagatt ccacaagtag atacaattgt gcgtggaaaa gattattgta atttttatcc
     1321 tgatagagta gcaatgcaag atgctactgc acaaatggct ttattgcaat ttatgaatgc
     1381 aggcaaacaa cgtgttgctg tgccatcgtc agttcattgc gatcatttaa taattgcaga
     1441 acaaggagag gttgctgatt tagctacagc aaacgacaaa aatgctgaag tatataactt
     1501 tttaagttca gtatcacaaa aatatggaat aggtttttgg aaaccgggtt ctggaattat
     1561 tcatcaaata attcttgaaa attatgccta tccgggcgga atgattatag gaacagatag
     1621 tcataccgtg aatggaggtg gcttgggaat gatagctatt ggagtgggag gtgcagatgc
     1681 ttgtgatgta atgagtggaa tggcatggga attaaaaatg cctaaaataa taggagtaaa
     1741 gttaaccggt acttgtaatc catgggtagc acctaaagat gtaatcttaa aattagcagg
     1801 aatacttact gtaaaagggg caacaaatgc agtattggaa tattttggcg aaggagcaca
     1861 aacattatcg tgtaccggaa aagctaccat atgcaatatg ggagcagaag taggagctac
     1921 aacttcaata tttgcttatg accaaagtat gaaagagtat ttgcatcata ctggacgaaa
     1981 agctgtagca gatttagttg aaacatattc tgaaatttta caagctgatg atgaggtatt
     2041 acaaaatcca cattcatatt atgattcaat tatagaaata aatctttcag aattagaacc
     2101 atatataaat ggaccattta ctcctgattt ggctacacct atttcaaaat ttgctgaggt
     2161 ggctcgtaaa gaaaaatggc ctttggaatt gaaagtagga cttattggtt catgcacaaa
     2221 ttcatcgtat gaagatttaa caaaggctgc atcattagct cggcaggcac aagaattaga
     2281 attagacata cagtcagaat ttttaatttc accggggtct gtaatgatta aaaaaacggc
     2341 aaaacgtgat ggtttattgg ctgcttttga agcaataggt ggcaaagttc ttgcaaatgc
     2401 atgtggtcct tgtattggtc aatggaatag agaaaatcct gaaccggaac ttaaaaattc
     2461 tatcataaca tcatttaata ggaactttgc aaaacgtgct gatggtaatc ccaatacata
     2521 ttcttttgtt gcatcacctg aaattgttac cgcacttact attgccggca gaattgattt
     2581 taatcctata accgacagtt taataaataa acacggtaag caagtgaaat tacaagctcc
     2641 acaagggaat gtaattcctg aaaagggatt tgaaatggta gatgcaggtt ttatagctcc
     2701 tgacgataaa aatgcacata ttgaaatctt gattaatccc caatcggaac ggttgcaaat
     2761 attacaacct tttgctcctt ggaatggaaa agattttatt ggattgcaat tacttcttaa
     2821 agctcaaggc aaatgtacta ccgatcatat ttcaatggca ggtccatggt tgcgatatcg
     2881 tggtcattta gaaaatatat cagataattt gctcatgggg gctgtaaatg catttaatgg
     2941 tcaaacaaat tcggtttgta atgcattaac aggtttatac gacaaagttt caagtgttgc
     3001 aaaggcatat aaacaaaaag ggttagcttc tattgtggta
//
LOCUS       DAOTOW010000223         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25580_length_3012_cov_3.658438, whole genome shotgun sequence.
ACCESSION   DAOTOW010000223 DAOTOW010000000
VERSION     DAOTOW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25580_length_3012_cov_3.658438"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="P5243_04950"
     CDS             complement(<1..355)
                     /locus_tag="P5243_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRS18828.1"
                     /translation="MNTNSKIDIHEFLNRDEKKDLLRLLTCGSVDDGKSTLIGRLMFD
                     SKMIYEDQLAALERDSKRIGHAGEEIDYALLLDGLKAEREQGITIDVAYRYFSTNKRK
                     FIIADTPGHEQYTRNM"
     gene            complement(392..1300)
                     /gene="cysD"
                     /locus_tag="P5243_04955"
     CDS             complement(392..1300)
                     /gene="cysD"
                     /locus_tag="P5243_04955"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRS18829.1"
                     /translation="MQEYSLSHLKELEAEAIHIIREVAAEFQNPVMLYSIGKDSSVMV
                     RLAEKAFYPGKVPFPLMHIDSKWKFKEMIEFRDSYARDNNWDLIVEYNKEAFEAGVGP
                     FTHGSKVHTDIMKTQALLAALDKHKFDAAFGGARRDEEKSRAKERIFSFRDKFHQWDP
                     KNQRPELWDIYNTKVHKGESIRVFPISNWTELDIWQYIRLENIPIVPLYYAKERPIVE
                     YNGTLIMVDDERMPQELAQKATMRKVRFRTLGCYPLTGAIESEADTIEKIVEEMMTVT
                     VSERTTRVIDFDQDGSMEQKKREGYF"
     gene            complement(1391..2380)
                     /locus_tag="P5243_04960"
     CDS             complement(1391..2380)
                     /locus_tag="P5243_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRS18830.1"
                     /translation="MNSFGRLFRVSIFGESHGIQIGAVIDGCPAGIELSEKDLEYDFS
                     RRRAGGLGTTPRIEPDLPRIVSGVYNGKTTGAPITVVFENTNTKSGDYSNLWDQPRPG
                     HADFTGQKKFGGYNDPRGGGHFSGRITLGLVAAGVIAKKIIAPATVSATLIEAGGSAD
                     IQKAVKEAVEQKDSIGGIIECICTGISTGLGEPFFDSVEALIAHMIFAVPATKGIEFG
                     SGFASARMKGSEHNDAIIDATGTTKTNHAAGINGGITNGNDIVFRVAVKPTSSIGIPQ
                     QTFNFKHNTIEELLIEGRHDACIALRIPVIIEAATAVALADLYLLSQQRSRVF"
     gene            complement(2689..>3012)
                     /locus_tag="P5243_04965"
     CDS             complement(2689..>3012)
                     /locus_tag="P5243_04965"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRS18831.1"
                     /translation="FILNIICIPYYGAYGAALASVITQYLTAIIQILLSKRIFHFTVQ
                     YKTIILLLVFTALTIGMCFYLKGVLSENWIYNMILLMIFSIIIAFSIRLIRIKDLVGI
                     VKYDI"
BASE COUNT          916 a          539 c          470 g         1087 t
ORIGIN      
        1 ccatgttgcg tgtatattgt tcatgaccgg gggtatcagc aataataaat ttacgtttgt
       61 tggtagaaaa atatcggtat gccacatcta tggtaattcc ttgttctctt tcggctttta
      121 atccatcgag taataatgca taatcaattt cttcgcctgc atggcctatt cgtttactgt
      181 cacgttctaa tgctgcaagt tgatcttcat aaatcatttt actgtcaaac atcaatcgtc
      241 ctatcagtgt tgattttcca tcatctaccg aaccgcatgt gagtaaacgt aataaatctt
      301 ttttctcgtc gcgatttaaa aattcgtgta tatctatttt tgagttcgta ttcattttta
      361 tagttttttg attgagctaa acctctaata attaaaaata tccttctcgt tttttttgtt
      421 ccatactacc atcttggtca aaatcaataa cacgagtggt tcgttcagaa acggttacgg
      481 tcatcatttc ttctactatt ttttcaatag tatctgcttc tgattctata gctccggtga
      541 gaggataaca gcctaacgta cgaaatctca ctttacgcat tgtagctttt tgagctagtt
      601 cttgaggcat gcgttcgtcg tctaccatta taagagtacc attatattct actattggtc
      661 gttcttttgc ataatacaaa ggaacaatgg gaatattttc taaacgaata tattgccaaa
      721 tatctaattc tgtccaatta gaaattggaa atacgcgtat tgattctcct ttgtgaactt
      781 tggtattata tatatcccat aattcaggac gttgattttt tggatcccat tgatgaaact
      841 tatcacgaaa cgagaatata cgttcttttg cacgtgattt ttcttcgtcg cgtcgggcgc
      901 ctccaaaggc tgcatcaaat ttgtgtttgt ccaaagctgc aagtaaggct tgtgttttca
      961 taatgtcagt atgtacttta cttccatgag taaatggccc aactccggct tcaaatgctt
     1021 ctttattata ttctactatc aaatcccaat tattgtcgcg agcatatgaa tcacggaatt
     1081 ctatcatttc tttgaacttc catttagaat ctatgtgcat aagagggaat ggtactttac
     1141 cgggataaaa agctttttct gctaatcgaa ccataaccga tgagtctttt ccaatagaat
     1201 agagcattac aggattttga aattctgcag caacttcacg aattatatga attgcttcgg
     1261 cttcaagttc ttttaagtgt gatagtgaat attcttgcat atatattatt ttttacacat
     1321 tgtatcctat ataattagta ggacaaatat aaatttttct ttcacaaaaa aaataatttt
     1381 ttttattgat ttaaaaaact cttgatcgtt gttgagataa taggtataaa tcggcaagag
     1441 caacagccgt ggcagcttct ataattaccg gaattctaag tgcaatacat gcatcgtgtc
     1501 gcccttctat aagtaattcc tcaattgtgt tatgtttaaa attaaatgtt tgttgaggaa
     1561 ttcctatact tgaagttggt tttacagcta ctctaaatac tatgtcattt ccattggtta
     1621 tacctccgtt aatacctgct gcgtgatttg tttttgtagt acctgttgcg tcaattatgg
     1681 catcattgtg ttcacttcct ttcatgcgag ccgatgcaaa tccacttccg aattcaatcc
     1741 cttttgtagc cggaactgca aatatcatat gagcaataag tgcttctact gaatcaaaga
     1801 atggttcacc taaaccagtt gatatgcctg tgcaaataca ttctataata ccacctatgg
     1861 aatctttttg ttctacagct tcttttactg ctttttgtat atcggcactt ccaccggctt
     1921 ctataagtgt tgccgataca gtagcaggag ctattatttt ttttgctatt acaccggcag
     1981 ctactaagcc aagtgtaatt ctacccgaaa aatgaccgcc tccccgaggg tcgttgtatc
     2041 caccaaattt tttttgacct gtaaaatcag catgccccgg acgaggttga tcccataagt
     2101 ttgaataatc gcctgatttg gtatttgtat tttcaaatac aacagtaata ggagctccgg
     2161 tagttttacc attgtataca ccgcttacta tacgtggtaa atcgggttct attcggggag
     2221 tagtacccaa acctccggct ctgcggcgag aaaaatcata ttctaagtct ttttcactta
     2281 attcaattcc tgcaggacaa ccgtcaatta ctgctcctat ttgaatacca tggctttcgc
     2341 caaatatact cactctaaat aatctaccaa aactattcat atttctatct tttatggtac
     2401 aaatgtacta aaagtttgac ttattatcat tagaatatct gtatgcaata taattatagg
     2461 taaaaacgct atattttatc actttgcatt ttcgttgcaa taaaatattg ttcaacaaaa
     2521 cttttttcat gcagtatcat cattgtttac tgaataaaac aattgagaaa atgagttttt
     2581 aaaatatatt ctgtataggg ttataaactg tagttgtaaa taaatttcaa aactaatata
     2641 gaataatttt ttgaacggta tttaaattcg tttcattttt taagtttatt aaatatcgta
     2701 ttttacaata ccaactaaat cttttatgcg aataagtctg atggaaaaag ctataataat
     2761 tgaaaaaatc ataagtaaaa tcatattata tatccaattt tcacttaaaa cgccttttaa
     2821 atagaagcac atacctatgg taagggcagt aaatacaagt aaaagaataa tagttttgta
     2881 ttgaacagta aaatgaaaaa tacgtttaga cagtaaaatt tgaataatag ctgttaaata
     2941 ttgagtaata acacttgcta atgctgctcc gtatgctcca taatatggaa tgcaaataat
     3001 atttaatata aa
//
LOCUS       DAOTOW010000224         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25583_length_3012_cov_3.028407, whole genome shotgun sequence.
ACCESSION   DAOTOW010000224 DAOTOW010000000
VERSION     DAOTOW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25583_length_3012_cov_3.028407"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..2674)
                     /locus_tag="P5243_04970"
     CDS             complement(35..2674)
                     /locus_tag="P5243_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS18832.1"
                     /translation="MKKVFTIAVCVVFAIVAYSQQIQQITVNATKNVMSVGETMQLTA
                     IISPQEVTNNTLTWSSSNPTIATVNTAGIVTAVSAGSVNIIASANDGSNVTGSFLLVI
                     SSVPITSITIGGSTAQMQMGQTIQLSATILPTNATNKSIYWSSLSPNVASVDQTGKVT
                     ALSTGTTTIKAMSQDGSGVYAIFDITVISYTTIENVAIPLKLYKTTVQSNQFMIDYEV
                     YPQTAPNKNVTWTSTNPQVASVDATGKVKPLSIGTTVIWAISQADITKKDSTVVEVEA
                     QQIAPTSIIISQKQLTLAPSSVPFKLYAEVLPLNATNKNVIWQVMNSNVVEMIAPGEF
                     IAKAPGTTEIIVTTEMGTIVTNNCYVIVQPIQAQEVVLSRKELTMQLGNTTQIQAYIK
                     PLQTTLKQLYWTSSNNSVVSVNTNGDILAVGVGTAYVYAQAIQTAGVKDSCLVTVVNE
                     EFIVHKQLPYATITIGNQQNTKFTISEYITYNGTQSVSYEAISTNPSVARAFVQGPIL
                     GIIPYTAGVTTIILQAKSASGLMQMVHATFTIESAVSTNICSSLQIDATIQNVSCAGG
                     TNGYAIVNASGGKAPYTYKWSNFRTDNAIKNVPAGQYRVVVTDANNCTKVEAISITEP
                     APMQINGTITQPTCGESNGAVTVTVEGGTAPYTYAWNIQSNMPNIIGLDAGLYSVIVK
                     DAQNCEAKRTFELNNTKGPIIFVKDITETNCNPNTGAIDIDVTGGTGVLDYTWSNGAK
                     TQDLSNVAAGNYSVTVTDENLCKSSAYITIPSIPFEQPDIALVTVGDTSKLNLIVWEK
                     EQTEAIDFYTIYRETSIAGVFESIGTQPYTAPSLFADPSAIPSERSYRYRISATHNCG
                     GESALSAQKAEYKTINLGTRLFF"
     gene            complement(2738..>3012)
                     /locus_tag="P5243_04975"
     CDS             complement(2738..>3012)
                     /locus_tag="P5243_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18833.1"
                     /translation="YSYDYPVSYDANTIAYISYAPKVRPAIEMATFASVITLAVIAPI
                     YAFNLKERNFNSDAYISIAAPAAVATIITLPLYYKIEERKLQFLQK"
BASE COUNT          946 a          519 c          493 g         1054 t
ORIGIN      
        1 aagttaaaag taaaaaatct atttttaact tgttttaaaa aaacagccta gtacctaagt
       61 ttatggtttt atattccgct ttttgagcac tcaacgctga ctcaccacca caattatgag
      121 tcgcagaaat tctatatctg taagaacgtt ctgatggaat tgctgaagga tcagcaaaca
      181 atgaaggtgc agtatagggt tgtgtaccaa tactttcaaa cactccagct attgatgttt
      241 ctctgtaaat agtataaaag tcgatagctt ctgtttgttc tttttcccaa actataagat
      301 ttaattttga tgtatcacct acagtaacca aagctatatc tggttgttca aaaggaattg
      361 aaggaattgt aatataagca gaacttttac ataaattttc atcggttaca gtaacactat
      421 aattaccagc agctacatta cttaaatctt gtgtttttgc accattgctc catgtgtaat
      481 ctaacactcc tgttcctccg gtaacgtcaa tatcaatagc tcctgtattt ggattacaat
      541 ttgtttcggt tatgtctttc acaaaaataa taggcccttt tgtattatta agttcaaacg
      601 ttctttttgc ttcacaattt tgagcatctt ttacaattac cgaatataat ccggcatcaa
      661 gtccaataat atttggcata ttactttgaa tattccatgc ataggtataa ggagcagttc
      721 ctccttctac agttactgtt acagcaccat ttgattcacc gcaggtaggt tgagttatag
      781 taccatttat ttgcattgga gcaggttctg taatagatat agcctctact tttgtacaat
      841 tatttgcatc ggttactact actctatatt gcccggcagg aacatttttt attgcattat
      901 cagttctaaa atttgaccat ttataggtat acggagcctt accaccactt gcatttacaa
      961 tagcatatcc attggttcca cctgcacatg atacattttg aattgttgca tctatttgca
     1021 acgaactgca aatattagta gatacggctg attctatggt aaaagttgcg tgaaccattt
     1081 gcattaaacc acttgctgat tttgcttgta atataatagt tgtaactccg gctgtatatg
     1141 gaataatacc aagtataggg ccttgcacaa aagctcgtgc tacagatgga ttagttgata
     1201 tagcttcata actaacagat tgagttccat tataggtaat atactcgctt atagtaaatt
     1261 tcgtattttg ctgattacca attgttattg tagcataagg caattgttta tgtacaataa
     1321 attcttcatt caccaccgta actaaacaac tgtctttaac tcctgcagtt tgaatagctt
     1381 gagcatatac ataagctgtt cctactccaa cagccaaaat atctccattt gtatttaccg
     1441 atactacaga attatttgaa cttgtccaat atagttgttt caatgtagtt tgcaaaggtt
     1501 taatatatgc ctgtatttga gtagtatttc ctaactgcat agttagttct ttacgagaca
     1561 atacaacttc ttgagcttga attggttgta ctatcacata acaattattg gttactatag
     1621 ttcccatttc ggttgttaca ataatttctg ttgtacccgg tgctttagct ataaattcac
     1681 ccggagcaat catttctaca acatttgaat tcattacctg ccaaataaca tttttattag
     1741 ttgcattgag tggtaatacc tctgcatata gtttaaatgg aacagaagaa ggagctaatg
     1801 ttaattgttt ttgagaaata atgattgaag taggtgctat ttgctgagct tctacttcta
     1861 caactgttga atcttttttt gtaatatctg cttgcgaaat tgcccatatt acagttgttc
     1921 caatactcaa aggtttaact ttaccggttg catcaacact tgctacttgc gggttagtag
     1981 aagtccatgt cacgttttta ttaggtgctg tttgaggata aacttcataa tcaatcataa
     2041 attgatttga ctgaactgta gtcttatata acttaagagg aattgcaaca ttttcaatag
     2101 ttgtatatga aattacagta atatcaaaaa ttgcgtaaac tccggaacca tcttgactca
     2161 tagctttaat agtagttgtt cctgttgaaa gagctgtaac tttacctgtt tgatcaacag
     2221 atgctacatt aggacttaaa cttgaccaat aaattgattt attggtagca tttgtaggta
     2281 aaattgtagc agataattgt attgtttgtc ccatttgcat ttgagcagtg gaacctccaa
     2341 tggtaatact cgtaattgga accgaactta ttactaacag aaaagaacct gtaacattag
     2401 aaccatcatt agccgatgca ataatattta cagaaccggc acttactgca gtaactatac
     2461 ctgccgtatt taccgtagct atggtaggat ttgaagaact ccacgttaac gtattattag
     2521 ttacttcttg aggactaata atagcggtaa gctgcatagt ttcaccaaca gacataacat
     2581 ttttagtggc atttactgtt atttgttgaa tttgttgtga atatgctact atagcaaata
     2641 caacacatac tgcaatggta aatacttttt tcataataat aattttttga tatatactaa
     2701 tgacaaatat accaaaaaat gccctacttt ggaagtatta tttttgcaaa aactgcaatt
     2761 ttcgttcttc aattttataa tacagaggta gagtaataat agtagccaca gcagcaggag
     2821 ctgctataga aatatatgca tcgctattga aattacgttc ttttaggtta aatgcataaa
     2881 taggagcaat tacagcaagg gtaataacag aagcaaaagt agccatttct atagccggtc
     2941 gtactttagg agcatatgaa atataggcta tagtattagc atcgtaactt accggataat
     3001 catatgaata ct
//
LOCUS       DAOTOW010000225         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25728_length_2999_cov_3.387228, whole genome shotgun sequence.
ACCESSION   DAOTOW010000225 DAOTOW010000000
VERSION     DAOTOW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25728_length_2999_cov_3.387228"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="P5243_04980"
     CDS             <1..308
                     /locus_tag="P5243_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRS18834.1"
                     /translation="NNIHKYLLKQVLYDYIPAELFQRPKWGFSIPLHSWLQNDLQFLI
                     HDYLNPVCIKKHGIFNPTIVQSIVKNYLVHKHEFLYNRIWCMILVQRFLEENIVMSK"
     gene            312..1547
                     /locus_tag="P5243_04985"
     CDS             312..1547
                     /locus_tag="P5243_04985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS18835.1"
                     /translation="MALSVLYISYDGMTDNLGQSQVIPYLIGLQKKGYSIHLLSCEKP
                     QVFEHRNNHVAKLLQKNSIQWYPIPYTAKPAVFSTMYDIKQLQKQASRIIKTHAISLI
                     HCRSYIAAQVGLYCNKKFGLPWIFDMRGFWADERIDGGIWNVKNPVYKTIYKHFKQLE
                     KKYIAKSSHIISLTHAGKHEIETWNVYKKNSTPITVIPCCADLEHFDSKVISKQKVIE
                     LQKKLHLSQNNFVLSYLGSFGTWYMTLEMFEFFKVLLNKLPDAIFLCITPDSPHMLEK
                     IARKKNIPLSSIRIVKAHREEVPLYASLSDWSLFFIKPLYSKKASSPTKMGELLGLGI
                     PLICNAGVGDVEEIMKDCSSGFVVKEYTLQEYEQIVDAIISRPTINHNQLYSVACTYY
                     SLQQGIHAYDLVYKQCIAL"
     gene            complement(1605..>2999)
                     /locus_tag="P5243_04990"
     CDS             complement(1605..>2999)
                     /locus_tag="P5243_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18836.1"
                     /translation="IGAPLAGFVSYDIFMKNGGVNDLITMGALQGKITTPTLFLLIAG
                     MVMVLTLWLSKKARMVTQTTLSLSNQSSGEEQFGSSVFARLIVRHSIRISENIGKFIP
                     KFIKKFVQKRFEQPKVRRKDASFDLLRASINLIVSSMLITLGTSLKLPLSTTYVTFMV
                     AMGSSLADGAWGRESAVYRITGVFTVIAGWFLTAFIAFSICAIIVYILYIGEIYAVIG
                     LFALSMFVLLKTQIFAKRKEAEKKQQEALKLDNTEENLEIRSHSNIALILNTTIDVFH
                     DINKGFANYNHQELKVAKQKAKELDKNAKVLKDSVNITIKDIGEEYFDASHNYVQVVD
                     YLREIARSLYFIADPSYSHVSNNHKPLLDIQKHELQEILEKFELYTRKIIHIIETKQF
                     SQIEDAINEQTLIFEYIQKVRKKQMKRAKTGEVNTRNSLLYLQILHEAKQILSYSMNV
                     LKSHRDLVKTIDFV"
BASE COUNT          999 a          447 c          466 g         1087 t
ORIGIN      
        1 ataacaatat tcataaatat ttactcaaac aagtattgta tgattatatt cctgcagaat
       61 tgtttcaacg tccaaagtgg gggttttcaa ttcctttaca ctcatggtta caaaatgatt
      121 tacaattttt aattcatgat tatttaaatc ctgtttgtat taagaaacat ggtatattta
      181 atcctactat agtacaatct attgtaaaaa actatttggt acataaacac gagtttttat
      241 acaatagaat ttggtgtatg atactggtgc aaagatttct ggaagagaat attgtaatgt
      301 caaaataagc tatggcacta tctgttttat atatttcata cgatggtatg accgataatt
      361 taggtcaatc acaggttatt ccgtacttaa ttggattgca gaaaaaagga tattctattc
      421 atcttcttag ttgtgaaaag cctcaagtat ttgaacatag aaataaccat gtggcaaaat
      481 tattacaaaa aaattcaatt caatggtatc caatacctta tacagcaaag cctgctgtgt
      541 tttcaaccat gtatgatata aaacagctgc aaaaacaagc ttctcgtata ataaaaactc
      601 atgcaatttc actaattcat tgtcgaagtt atatagctgc tcaagtgggt ttatattgta
      661 ataaaaaatt tggattgcct tggatttttg atatgcgtgg tttttgggcc gacgaacgta
      721 ttgatggagg aatttggaat gtaaaaaatc ctgtatataa gactatttat aaacatttta
      781 aacaactcga aaaaaagtat attgcaaaat cttcacatat tataagttta actcatgcag
      841 gaaaacatga aattgaaaca tggaatgtgt acaaaaaaaa ttccactcca attactgtta
      901 ttccttgttg tgccgattta gaacattttg attctaaggt tatatcaaaa caaaaagtta
      961 tagaattaca aaaaaaattg catttgtctc aaaacaactt tgttttaagt tatttagggt
     1021 catttggaac atggtatatg acacttgaaa tgtttgaatt ttttaaggta ttgctaaata
     1081 aattaccgga tgcaatattt ctttgtatta ctcccgattc tcctcatatg ctcgaaaaaa
     1141 ttgctcgtaa aaaaaatata cccctttctt ctattcgtat tgtaaaggca catagagaag
     1201 aagtgccatt gtatgcatcg ctttctgatt ggtcactatt ttttattaaa ccattatatt
     1261 ctaaaaaagc ctcatctcct accaaaatgg gagaattgtt aggacttggt attccgctga
     1321 tttgcaatgc cggggtgggc gatgttgaag aaataatgaa agattgttcg tcaggatttg
     1381 tggttaagga atataccctg caagaatatg aacagattgt tgatgctata atttctcggc
     1441 ctactataaa tcataatcaa ttatattcgg ttgcatgcac ttattattcc ttacagcaag
     1501 gaatacacgc atatgattta gtttataagc aatgtattgc attataataa aaaagaggct
     1561 ttctataaga agaaagcctc tttttttaat tatacggtta attattatac aaaatctatt
     1621 gttttaacta aatcgcggtg cgatttcaaa acattcatag aatatgataa aatctgtttt
     1681 gcttcgtgta aaatttgtaa atacaataaa ctgtttcgcg tatttacttc accggttttt
     1741 gctcgtttca tttgtttttt tcttactttt tgaatgtatt caaaaataag ggtttgttca
     1801 tttatagcat cttctatttg agaaaattgt tttgtttcta ttatatgaat tattttgcgg
     1861 gtatataatt caaatttttc taaaatttct tgtaattcat gtttttgaat atcaagtaac
     1921 ggtttatggt tattgcttac atgagaatat gaagggtctg ctataaagta taggctacgt
     1981 gcaatttcac gtaaataatc aactacttga acgtagttgt gacttgcatc aaaatattct
     2041 tcgcctatat ctttaattgt tatattaacg gaatctttca atactttggc gtttttgtca
     2101 agttctttag ctttttgttt tgctactttt aattcttgat gattgtagtt tgcaaatcct
     2161 ttgttaatat catgaaatac gtcaattgtt gtattaagta taagtgcaat atttgaatgt
     2221 gaacgtattt caagattttc ttccgtattg tcaagtttta atgcttcttg ttgttttttt
     2281 tctgcttctt tacgttttgc aaaaatttgt gttttaagca aaacaaacat tgaaagagca
     2341 aataatccta taacggcata tatttcacct atatataata tatacactat tatagcacaa
     2401 atactaaatg caataaaagc agttaagaac caacctgcaa ttacagtgaa aacacctgtt
     2461 attctataaa cggcagattc gcgcccccat gcaccgtcag ccaatgatga tcccatagca
     2521 accataaatg taacatacgt agtagaaaga gggagtttaa gagaagttcc taatgtgata
     2581 agcatactgg aaacaataag atttattgat gctcttagta aatcaaatga tgcgtctttt
     2641 cgtcgaactt ttggctgttc aaatcgtttt tgaacaaatt tcttaataaa tttaggtata
     2701 aacttaccaa tattttcaga aattcgaata ctgtgtctta caattaaacg tgcaaatact
     2761 gaagatccaa attgttcttc accacttgat tgattactta aacttaaggt agtttgggta
     2821 accattcgtg ctttttttga aagccataat gttagaacca ttaccattcc ggctattaat
     2881 aaaaaaagtg taggtgtagt aattttaccc tgaagcgccc ccattgtaat taagtcgttt
     2941 actccgccat ttttcatgaa tatatcgtat gaaacaaatc cggctaaagg agctcctat
//
LOCUS       DAOTOW010000226         2991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25818_length_2991_cov_3.227520, whole genome shotgun sequence.
ACCESSION   DAOTOW010000226 DAOTOW010000000
VERSION     DAOTOW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25818_length_2991_cov_3.227520"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="P5243_04995"
     CDS             complement(<1..1114)
                     /locus_tag="P5243_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS18837.1"
                     /translation="MKIVHLIIVYIFSVASLFSQVDTSLSKEIIKKNWNIGGIPVIAY
                     NSDLGLQYGVIINPFYYGDGSTYPNYVHKLYFEVSRYTKGSGIYRFYYNSKYLIPTIN
                     TFFDISYLTDDAYDFYGFNGRQSLYNALWIAPQSLTYVGSYFYDIRRTMIRVKADFQG
                     KTIIPYLGWIAALQYRKISIDRPNFAKINRGKSIENYIPSDSILYDKYVLWNIIPKEQ
                     SSGGIVTNAKCGLLYDTRDVESNPSRGIWTEAVFTVYPQFLNSYTSFGICNISHRQYI
                     TLVPRRVIFAYRTIFQSHVFGKVPWYILPDIETSVLTAATNEGLGGAASLRGILRNRI
                     VGKQTIFGNFELRYKFVSFRIKKQNVYIATNLFYDAG"
     gene            complement(1125..1979)
                     /gene="dapF"
                     /locus_tag="P5243_05000"
     CDS             complement(1125..1979)
                     /gene="dapF"
                     /locus_tag="P5243_05000"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRS18838.1"
                     /translation="MQTNFFVKSHGLGNDYIVIDSEHIQFELKPQIITKICDVHYGIG
                     SDGILVKVPSTIADFGLLIYNTDASVAENCGNGLRIFSKFLFDYGYVSNNSFTVDILG
                     RLIHCEVIETKNNKATKIKVDMGKANFIASEVPVIFHKQECIHEKYTFGDTEFVINCV
                     SVGNPHCVVFRDELIQEELLTYGPIIEKHTMFPNRINVQFAKVIDRHTVEIRIWERGV
                     GNTLASGSSSCAVASVMKKNNLVDSPVTVKMPGGELLIAIDNEWNIQMTGPVQEIASG
                     ILSPELFE"
     gene            complement(2062..2547)
                     /locus_tag="P5243_05005"
     CDS             complement(2062..2547)
                     /locus_tag="P5243_05005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRS18839.1"
                     /translation="MNIVYCSIDSVDDDSLFCLFSDNELLVYTDVPIIRSIEQNAEIR
                     NYYTFPRIIFFIKISQYIVGFVSAYCNIKHKTACITCVLQPQFQKQGIMHTTLVWFHN
                     YLVVNYSIVRIEAQVYTEHSDSIAMLESLGYTREGCLRKNFMIQGTLCDSYMYSLLCD
                     N"
     gene            complement(2623..>2991)
                     /locus_tag="P5243_05010"
     CDS             complement(2623..>2991)
                     /locus_tag="P5243_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18840.1"
                     /translation="VDNTDLKDAKNPLDAARIVKLGLTFDDGGQGTLNDAKTLYIKDI
                     VIGKAANPQVYAPWASLETVSGAALQVVDGVIYSAGAIKVTNAAGQVVKVAKKTLEIS
                     SLPAGVLVITAAEGTAKIVK"
BASE COUNT         1049 a          537 c          398 g         1007 t
ORIGIN      
        1 ctccggcgtc ataaaaaaga ttggtagcta tatatacatt ttgttttttt attcgaaaag
       61 aaacaaattt atatcgaagt tcaaaattac caaatatagt ttgttttcct acaattctat
      121 tacgcaaaat tccacgtaaa cttgccgctc cgcctaatcc ctcatttgta gcagcagtaa
      181 gtactgatgt ttcaatatca ggcaatatat accatggtac cttaccaaaa acatgcgact
      241 gaaatattgt tctatatgca aagattacac gacgaggaac caatgtaatg tattgacgat
      301 gacttatatt gcatattcca aaacttgtat aggaattcag aaattgagga tacaccgtaa
      361 acaccgcttc tgtccaaatt ccgcgactag gatttgattc tacatcacgc gtatcataca
      421 ataacccaca tttagcattg gtaactatac ctcccgatga ttgttctttt ggaattatat
      481 tccataagac atacttatca tataatatag agtcggaagg tatataattt tcaattgatt
      541 ttcccctatt tatttttgca aaattaggtc tgtcaatact aatttttcta tactgtaaag
      601 ctgctatcca acctaaatac ggaataatcg tttttccttg aaaatcggct tttacccgta
      661 tcattgttct gcgtatatca taaaaatatg aacctacata tgtgagtgat tgtggggcaa
      721 tccacaatgc attgtacaat gattgtcgtc cgttaaaccc ataaaagtca taggcatcgt
      781 ctgttaaata actaatatca aaaaaagtat tgatagtagg aattaaatat tttgaattgt
      841 aataaaacct ataaattcca ctgccttttg tatatcgtga tacttcaaaa tataatttat
      901 gtacatagtt aggatacgtg gaaccatcac cataataaaa cggatttatt attactccat
      961 actgtaaccc taaatctgaa ttgtatgcta ttacaggaat tcctcctata ttccaatttt
     1021 ttttaataat ttcttttgaa agtgatgtgt caacttgtga aaacaaagat gcaacactaa
     1081 aaatataaac aataattaag tgtactattt tcatgtaaca tgttttattc aaataattct
     1141 ggactcaaaa ttcctgatgc aatttcttgt accggtccgg tcatttgaat attccattca
     1201 ttatcaatag ctataagtaa ttcacctccg ggcattttaa cagtaacagg tgaatctacc
     1261 aaattatttt ttttcattac acttgccaca gcacatgaag aactccctga agctaatgta
     1321 tttcctactc cacgctccca aattctaatt tctaccgtat gtctatctat tacttttgca
     1381 aattgtacat taattctatt gggaaacatt gtgtgtttct ctataatagg tccatacgta
     1441 agcaattctt cctgaattaa ttcatcgcga aatacaacac aatgaggatt tccaaccgaa
     1501 acgcaattaa taacaaactc tgtatcacca aatgtatatt tttcatgtat acattcttgt
     1561 ttatgaaaaa ttacgggtac ttcagatgca ataaaatttg ctttccccat gtctacctta
     1621 attttagtag ctttattgtt ttttgtttct atcacttcgc agtgtattaa acgaccaagg
     1681 atatcaacag taaaagaatt atttgaaaca taaccgtaat caaataaaaa cttactaaat
     1741 attcgcaatc cgtttccgca attttcggca accgatgcat cggtattata tataagtaaa
     1801 ccgaaatctg caatagttga aggaacttta accaatatgc catcagaacc tataccgtaa
     1861 tgtacatcgc atattttagt aattatttgc ggcttcaatt caaattgaat atgctcagaa
     1921 tcaattacaa tataatcatt accaagccca tgtgatttaa caaaaaaatt agtttgcata
     1981 catttagtaa tttatatgca atatactaaa aaagtataca tgaatgaact gaaattgttt
     2041 gatgttttga gaaacaataa tttaattatc acacaacaaa gaatacatat atgaatcaca
     2101 taatgttcct tgaatcataa aatttttacg caaacacccc tctcgtgtat accctaatga
     2161 ttcaagcata gcaatagaat cagaatgttc tgtatatact tgtgcttcaa ttctaactat
     2221 tgaataattc acaactaaat aattatgaaa ccataccaac gttgtatgca ttattccttg
     2281 cttttggaat tgaggttgta aaacgcacgt aatacatgca gttttatgtt ttatattaca
     2341 atatgccgaa acaaaaccaa caatgtattg cgaaatttta ataaaaaaaa taatacgcgg
     2401 aaaagtataa tagtttcgta tttctgcatt ttgttcaata gaacgtatta ttggcacatc
     2461 ggtataaacc aacaattcat tatcagaaaa taagcaaaat aatgaatcat catcaaccga
     2521 atctatagaa caatacacaa tattcatatc attgaaaaaa agacataaaa aaaccccact
     2581 cataagagtg aggtttttat attgtaatgt aattacttag aattatttta cgattttagc
     2641 tgttccttca gcagcagtga ttacaagaac accagctggt aatgaagaaa tttctaatgt
     2701 ttttttagca acttttacaa cttgacctgc agcattagtt actttgatag cacctgctga
     2761 atagattaca ccgtcaacta cttgtaatgc agcaccagaa actgtttcta aagaagccca
     2821 tggagcatat acttgtggat tagcagcttt acctattacg atatctttaa tataaagagt
     2881 tttagcatcg tttaatgtac cttgtccacc atcatcaaaa gttaaaccta attttacgat
     2941 tctcgcagca tcaagtggat ttttagcatc tttcaaatca gtattatcaa c
//
LOCUS       DAOTOW010000227         2979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_25943_length_2979_cov_2.620383, whole genome shotgun sequence.
ACCESSION   DAOTOW010000227 DAOTOW010000000
VERSION     DAOTOW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25943_length_2979_cov_2.620383"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="P5243_05015"
     CDS             <1..1711
                     /locus_tag="P5243_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS18841.1"
                     /translation="TVRANVVGGHPGYTYSWTYNGIPQQPKTADIKNMPAGTFTVVAK
                     DTRGCLSEEATYVMNQPPKIQAHFTVELTGCGTLASTGSIKLDTIIGNNPPYRFRWHN
                     ETTTSTWFKDKTNRIKTNLPAGEYFFTVLDSLGLCYNVFANYTNPIVVSSIDTIITHT
                     RCNYHTDEELSANKPEGSIEIVSITTKRGNYNDPAEYQTLSNFPEYTIRWEDSRNQTT
                     RKATHLEQNTNGYFVNLTGPNQCVSRMPAGRVDALINLDAKIMSPDDNVYSKHTICLE
                     DSIQLEADVQESFTHNYTPVNTSKTYTWNTIAANKLSDLSSKIAKTIWVNPLTSYYRD
                     SSLVTMFYEFDGCTSPLSNYVINHYDSIQFALEVLDVFDNYLGTDSVFAIQKEQFNIV
                     PTVEPWYVLKDVDSDGIMSINWTSKNPAKTGQGQLADTVTNEATYIASSNYGLKLTAK
                     EPSYYFAEAVTTHGCKQKASVFVNVYSSLFIPTGITPNGDGQNDTWIIPYLYMCPNAV
                     VKVYNRWGVKVYENEGEYYKHPWNGTNKNGKQLPMGAYYYIIEYNDVNNTPPQAGSIS
                     ILY"
     gene            1718..2617
                     /locus_tag="P5243_05020"
     CDS             1718..2617
                     /locus_tag="P5243_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRS18842.1"
                     /translation="MKRIVIYTFLLILSLTALGQQTPMYTLYTFNDFLINPAIAGTKN
                     YYEAKVNNRYQFAGIENAPVTASLSLHGRYGNLPMGVGGIVYNDSQGAFSKFGVYGAY
                     SYILPIDNRTDFSLGMNVGLVEYKIDISRITFIEPEYGIDETLYRYLRPDATFGAYIV
                     SRDYYAGLSLDQLFNNKIEVINDTLVIDNSTVNRITSHASVIAGTKMKLSYGFVFEPS
                     VVVRKTVRTPVQLELTGCLTYEKSVWAGVSVRSGDAIALLLGYNYRRLLTFGYAYDIT
                     YSTLRKASNGSHEVMIGVKFNER"
     gene            2627..>2979
                     /locus_tag="P5243_05025"
     CDS             2627..>2979
                     /locus_tag="P5243_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRS18843.1"
                     /translation="MKQFLVYLFCFVLFACNSGKKNEVNFEKIPESIMVKILYDIHFH
                     DGVVNSYNNMDKPNIFLSKEHYETKIHQKYGYSDTLFKMNIEYYTMNMKIKDIYTQVI
                     DSMNAQKAKLEQRRQ"
BASE COUNT         1062 a          408 c          524 g          985 t
ORIGIN      
        1 aactgtgaga gcaaatgtgg ttggcggtca tccgggttat acatattctt ggacctacaa
       61 tggaatacca cagcaaccaa aaacggccga cattaaaaat atgcctgcag gaacatttac
      121 tgtagtagca aaagacacta gaggttgttt aagtgaggag gctacttatg taatgaatca
      181 accaccaaaa attcaggccc attttactgt tgaacttacc ggatgtggta ctcttgccag
      241 tacaggttct ataaaacttg atactattat aggtaataat ccaccgtatc gttttcgttg
      301 gcacaatgaa actacaacgt caacatggtt taaagataaa actaaccgaa ttaaaacgaa
      361 tttacctgcg ggagagtatt tctttactgt attagattcg ttagggttat gttataatgt
      421 atttgctaat tatactaatc ctattgtagt tagtagtatt gatactatta taactcatac
      481 ccgttgtaat tatcataccg atgaggaatt aagtgctaat aaacctgaag gtagtattga
      541 aatagtaagt attacaacaa aaaggggtaa ttataatgat cctgcagaat atcaaacttt
      601 atcaaatttt cctgaatata caattcgttg ggaagattct cgtaatcaaa caacacgtaa
      661 agcaactcat ttagagcaga atacaaacgg atattttgta aatcttaccg gaccaaatca
      721 atgtgttagc agaatgcctg ccggtagagt tgatgcgttg attaatcttg atgctaaaat
      781 tatgagtcct gatgataatg tatattcaaa acacactatt tgtttagaag attctattca
      841 attagaagca gatgtgcaag aatcattcac acataattat acccctgtaa atacttctaa
      901 aacatataca tggaatacaa ttgctgccaa taaattatca gatttatcgt caaaaattgc
      961 taaaactatt tgggtaaatc cattaacgtc atattacaga gatagttcat tagttactat
     1021 gttttatgaa tttgatggat gtacatcacc gttgtcaaat tatgttataa atcattatga
     1081 ttcaatacaa tttgcattag aagttcttga tgtatttgat aattatttgg gaactgattc
     1141 tgtatttgct attcaaaaag aacaatttaa tatagtgcca accgtagagc catggtatgt
     1201 gttgaaggat gtagattctg atggtattat gtcaataaat tggacttcaa aaaatcctgc
     1261 aaaaaccgga caaggtcaat tggcagatac tgtaacaaat gaagctacat atatagcttc
     1321 gagtaattat ggtttaaaat taactgcaaa agaaccaagt tattattttg cagaggctgt
     1381 tactactcat gggtgtaaac aaaaagcaag tgtatttgta aatgtatatt caagtttatt
     1441 tatacctacc ggtataacac caaatggtga tggacaaaac gatacatgga ttataccata
     1501 tttgtatatg tgcccaaatg ctgttgttaa ggtttataat cggtggggag ttaaagtcta
     1561 tgaaaatgaa ggagaatatt ataaacatcc ttggaatgga actaataaaa atggaaaaca
     1621 attgcctatg ggagcatatt attatattat agaatataat gacgtaaata atactccgcc
     1681 acaggcagga tcaatttcta tactttatta aaaatacatg aaacgaatag ttatatatac
     1741 ttttttattg attttgtcgc ttactgcatt aggacaacaa acaccaatgt atactctgta
     1801 tacttttaat gattttttaa ttaatcctgc aatagcagga actaaaaatt attatgaggc
     1861 aaaagtaaat aatagatatc aattcgcagg catagaaaat gcacctgtta ctgcatcact
     1921 cagtttacat ggtagatatg gtaatttacc aatgggagtc ggtggtatag tatataatga
     1981 tagtcaagga gctttctcaa aattcggtgt ttatggagca tattcatata ttttgccaat
     2041 tgataatagg acagattttt cattgggaat gaatgttggt ttagtagaat ataaaattga
     2101 tatttctcga attacgttca ttgagcctga atatggaatt gatgaaacat tatacagata
     2161 tttgcgtcct gatgctacgt ttggtgctta tatagtgtct agagattatt atgcaggatt
     2221 atcgttagat caattattta acaataaaat agaggtaata aacgatacat tagttattga
     2281 taattctaca gtaaacagaa ttaccagtca tgcttctgta atagcaggta caaaaatgaa
     2341 attaagttat ggttttgttt ttgaaccaag tgtagtggtt cgtaaaactg ttagaacgcc
     2401 ggtacaatta gaattaacag gatgtttaac ttacgaaaaa tcagtttggg caggagtttc
     2461 agttcgttcc ggtgatgcta ttgcattatt acttggttac aattatcgta ggctattaac
     2521 atttggatat gcttatgata ttacctattc aactttacgt aaagcttcaa atggatcaca
     2581 tgaagtaatg attggcgtta agtttaacga acgataaatg ggtgctttga aacagttttt
     2641 agtgtatttg ttttgttttg ttttatttgc ttgtaattca ggcaaaaaaa atgaagtaaa
     2701 ctttgaaaaa attcctgaat ccataatggt aaaaatttta tatgacattc attttcacga
     2761 tggtgttgtg aattcatata ataatatgga caaacctaat atttttttat caaaagaaca
     2821 ttatgaaaca aagattcatc aaaagtacgg atattcagat actttgttta aaatgaatat
     2881 tgagtattat actatgaata tgaaaattaa ggatatatat acacaagtaa ttgattctat
     2941 gaatgctcaa aaagctaaat tggaacaacg aagacaaca
//
LOCUS       DAOTOW010000228         2972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26012_length_2972_cov_3.668495, whole genome shotgun sequence.
ACCESSION   DAOTOW010000228 DAOTOW010000000
VERSION     DAOTOW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26012_length_2972_cov_3.668495"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="P5243_05030"
     CDS             complement(<1..1082)
                     /locus_tag="P5243_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18844.1"
                     /translation="MNRETKNVLQQIIFIGNSSDTISDMMQRVCNYLFQNWFISTVSG
                     VEIVCEGILYSNTECKISPYFQKQSIEIQKNILGEITIYYKSITEFSSFLSDDLQQIA
                     AMISGFIAQKKLSVISYDYTERIKELHGITKTTHIFKQNKSIENKLQEICNYIPQTMQ
                     YPQYTVVRIVYDKEEFVSPHFVKTEWVLKQNFKATNNKQGKIEIYYTKEFKEAYKGPF
                     LKEEIELLHNICMLIVATINQEVLRDLLYQNRERLKELDGINKTRYILNQAKPISETL
                     QNICEILPASWQYPEYTCSCITYENIQYISSNFSRTPWCLKETFITFDNKIGSIEIFY
                     TKEFPHCYEGPFLKEERNLLTNIANL"
     gene            complement(1230..2696)
                     /locus_tag="P5243_05035"
     CDS             complement(1230..2696)
                     /locus_tag="P5243_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18845.1"
                     /translation="MKKIVISILLVSALQYAWSQDYEDLLKFSQSNQIGNARYSAMSG
                     AFGSIGANASAMSINPAGSALLRGSLFEFTPEFTLTKTENHYLGNYDRAFTGSLKIPN
                     IGFMSGKNIKESDLFITGVSFGFSINRQNLYNQTSNFNARNNGSSITDDFLRLANKDL
                     WYSDYNDLAYDSKLIFFDTVSDMYVSDYRWYDGNVVKTQYGQDQAVNLKKSGSKREYL
                     ANFGIDFSQKVYLGANIAAESIFYTEDFKFTEIDNQQKLYYLSDFTYSKSTELVGMGV
                     NAKVGVIVRPVEYLRLGLAAHSPSVYSMSTTTTSTLHVNYENYEVPNKSAENINEFDY
                     QMLTPSKIVASLGFVYKNLAVLGVDYESVNYSYGYLNLNSPDEAARNQAIADNLTKTD
                     NLKVGGELRYGPFSFRGGFATYGNPYSKYVVDDPFIRTDVSGGVGIGNDRFYCDLAWV
                     RSNTKQYYSLYSNYSGSEVSSRTNLKRDNLLATIGFKF"
     gene            complement(2718..>2972)
                     /locus_tag="P5243_05040"
     CDS             complement(2718..>2972)
                     /locus_tag="P5243_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18846.1"
                     /translation="PRSGGSHSEVYRKTETTSQPSRNSSYRGPQDYSSKNGSSSYGSS
                     SQSYNKPASSKSNSNVQVPQRGYSTSKPSSSSSSRTSYSR"
BASE COUNT         1004 a          491 c          348 g         1129 t
ORIGIN      
        1 attaaatttg caatatttgt aagtaaattt ctttcttctt ttaaaaatgg tccctcatag
       61 caatgaggga actctttagt ataaaatatt tctatactac ctattttatt atcaaaggtt
      121 ataaaagttt cctttaaaca ccaaggcgtt ctactaaaat ttgaacttat atattgaatg
      181 ttttcatagg taatacagct acaagtatat tcaggatact gccatgatgc aggcaatatt
      241 tcacatatat tttgtaaagt ttccgatatt ggttttgctt gatttaaaat atatcttgtt
      301 ttatttattc cgtctaattc tttaagacgt tctctgtttt gatacaataa atctcttaat
      361 acttcttgat taatagtagc aactattaac atacaaatat tatgtaacaa ctctatttcc
      421 tcttttaaaa aaggtccttt ataagcttct ttaaattcct ttgtataata tatttctatt
      481 ttaccttgtt tattattagt tgctttaaaa ttttgtttca atacccattc tgtttttaca
      541 aaatgcggag aaacgaattc ttctttatca tatacaattc ttacaacagt atattgagga
      601 tactgcattg tttgtggtat atagttacat atttcttgta atttattttc tatactttta
      661 ttttgtttaa atatatgtgt tgtttttgta ataccatgca attcttttat tcgttcggta
      721 taatcatatg aaataaccga taattttttt tgtgcaataa aaccactaat cattgcagct
      781 atttgttgta aatcatcaga aagaaacgaa gaaaattctg taattgattt ataatatatt
      841 gtaatttccc caagtatatt tttttgaatt tctatagatt gcttttgaaa ataaggagaa
      901 attttgcatt cagtattact atataatatt ccctcacata ctatctcaac acccgaaact
      961 gttgaaataa accaattttg gaataaataa ttacatacac gttgcatcat atcagaaata
     1021 gtatcagaac tgtttccaat aaatataatt tgttgtaata cattctttgt ttctctattc
     1081 attttagcaa attacagtta ctgtattcta ttaaaaaatc aatagttcca ataaattatt
     1141 cgaatatata attttttttt attgttaaaa aaaaaagagc ggtataagtt tcttataccg
     1201 ctcttaatag aatctatact aatatttgat taaaatttaa atcctatagt tgctaataaa
     1261 ttatcacgtt taagattagt tcttgaagag acttcacttc cactataatt actatataaa
     1321 ctatagtatt gttttgtatt tgaacgtacc catgctaagt cacagtaaaa tctgtcattc
     1381 ccaattccaa caccaccaga cacatcggta cgtataaaag gatcatctac cacatatttt
     1441 gaatatggat ttccatacgt tgcaaaacca cctctaaaac taaatggtcc atatcgcaat
     1501 tctccaccaa cttttaaatt atctgttttt gttaaattat ctgctatagc ctgatttcga
     1561 gctgcctcat caggcgaatt taaatttaaa taaccataag aataatttac tgattcataa
     1621 tcaactccta acacagccaa atttttgtat acaaaaccta aactcgcaac aatttttgat
     1681 ggtgttaaca tttgataatc aaattcattt atattttcag cacttttatt tggaacttca
     1741 taattttcat aatttacatg caaagttgat gttgttgttg tactcataga ataaactgat
     1801 ggcgaatgag ctgcaagtcc taatctcaag tattctacag gtcttacaat aacccctact
     1861 ttagcattta ctcccatacc aactaattca gtagatttac tataggtaaa gtcacttaaa
     1921 taatacaatt tttgttgatt atcaatttcg gtaaacttga aatcttctgt ataaaaaata
     1981 ctttctgcgg caatatttgc tcctaaatat actttttgtg aaaagtcaat tccaaaattt
     2041 gccaaatatt ctcgttttga acctgacttt ttaagattta cagcctgatc ttgaccatat
     2101 tgtgttttaa ctacattacc atcgtaccaa cgataatcgc taacatacat atcactaact
     2161 gtatcaaaga atattaattt tgaatcataa gccaaatcat tataatctga ataccataaa
     2221 tccttatttg ctaatcttaa aaaatcatcg gttatagaac ttccattatt tcttgcatta
     2281 aaattagatg tttgattata tagattttga cgattaatag aaaaaccaaa tgatactccc
     2341 gttataaaca aatcactttc ctttatattt ttacctgaca taaaaccaat atttggaatt
     2401 tttaaagaac ctgtaaatgc cctatcataa ttacccaaat aatgattctc tgttttggta
     2461 agtgtaaatt ccggagtaaa ttcaaacaaa gaacctcgta acaatgcaga accggctgga
     2521 tttatactca ttgcagaagc attggctcca atagaaccaa aagctcccga catagcagaa
     2581 taacgtgcgt taccaatctg attactttga ctaaatttta ataaatcttc gtaatcttgt
     2641 gaccatgcat actgtaatgc actcaccaat aatatactaa ttacaatttt tttcatacta
     2701 ttacattttt taatttatta tctactatat gatgttctcg aagaacttga actacttggt
     2761 tttgaagtag aataacctct ttgaggaact tgaacattac tatttgactt actactggca
     2821 ggtttattat atgattgtga actactacca tatgaactac taccattttt tgaagaataa
     2881 tcttgtggac ctctatagct actatttcta cttggttgag aagtcgtttc agtttttcta
     2941 tacacttcag aatgagagcc tccagaacga gg
//
LOCUS       DAOTOW010000229         2971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26023_length_2971_cov_3.811728, whole genome shotgun sequence.
ACCESSION   DAOTOW010000229 DAOTOW010000000
VERSION     DAOTOW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26023_length_2971_cov_3.811728"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="P5243_05045"
     CDS             <1..539
                     /locus_tag="P5243_05045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRS18847.1"
                     /translation="LHGFHFLSKEGTMAIILPHGVLFRGGSEERIRRKLLEDGNIDTV
                     IGLPANLFFSTGIPVCILVLKKCKKFDDVLFINAVDHFEKGKRQNTLLPEHIDKIVNT
                     YKGRKEETRYSRRVSMDEIEKNEFNLNISRYVSTSVDEEIIDLKEVNKKLAELDKEIN
                     KARDTHNKYLNELGLPPI"
     gene            complement(777..917)
                     /locus_tag="P5243_05050"
     CDS             complement(777..917)
                     /locus_tag="P5243_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18848.1"
                     /translation="MRSCIYRLAVWLVADFEALSYQVTTTFDTSCAARIPTAPAINYT
                     AY"
     gene            1597..2145
                     /locus_tag="P5243_05055"
     CDS             1597..2145
                     /locus_tag="P5243_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS18849.1"
                     /translation="MLSDIKSFDYTIYLKVILPAIPGVIIGSYVIELVSVHSMKLFFG
                     LFLVVFTIYTIFKLQIPKILENFLLIINGLIHGLLGVGGPIPVAIYKNKINKSSLRYT
                     FAVVFITMNVMRIVQYSIQKTYNFKLLLNYWLLPFLLLVPAFLGYKIHVKISEKNFRL
                     GLSIILLIIGLYFLIRSFIAIT"
     gene            complement(2315..>2971)
                     /locus_tag="P5243_05060"
     CDS             complement(2315..>2971)
                     /locus_tag="P5243_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS18850.1"
                     /translation="NMALCRIIGLDLYTPLIYDTIIPIDTLIWKHLQGTESIQKRPEY
                     SILQNKIAYKEQELKMTRATYLPTIGLKGGYTHIGGIQINSQDYSNSNVQFIVSVNIP
                     LFAWTEGKQKLASAQIDIKNQELELEKNSELMKLELEKTKQDMKHALTQIRINEKAVM
                     QSQENLRITQNNYIIGKIPVSDVLTAQTLWQQASTNLIQSKIDYANYVCMYKKLIGIL
                     "
BASE COUNT          975 a          438 c          501 g         1057 t
ORIGIN      
        1 ttttgcacgg cttccacttc cttagtaaag aggggaccat ggcaattatt ttgccccacg
       61 gcgttttgtt caggggcgga tcggaagaac gcatcaggag aaaattatta gaggacggaa
      121 acattgacac ggtaatcggg ctacctgcca acttgttttt ctctacaggt attccggttt
      181 gtatattggt tttaaagaag tgcaaaaagt tcgacgatgt attgttcatc aacgccgttg
      241 accattttga aaaggggaaa cgacaaaaca ccctgctccc cgaacacatt gacaagattg
      301 ttaacaccta caaaggccgt aaagaagaaa cccgttactc ccgcagggtt tcaatggacg
      361 aaatcgagaa aaacgaattc aacctgaata tttcacgcta tgtaagcaca tcggttgacg
      421 aagaaataat tgacctgaaa gaggtaaaca aaaaattggc cgaacttgac aaagaaatta
      481 acaaagcaag agatacccac aataaatatt tgaatgaatt ggggcttcca ccgatataaa
      541 gagccaacgc tatttgcaag cacattgcca aagccccacc cacgctacag ccaaagcttt
      601 gtcaaagagc ttgcaaatcc agcccgagta ccgcctttca ggtcggttta gggtttaccc
      661 acgctcaaaa aaacaaaata aatttgtgct tcgtggacag gttggtacgt tttgatagag
      721 attaaaaaga gagctaaatg attgacaaac agacggaaaa caatgaacac cagcccctaa
      781 taagcggtat agttaattgc cggtgcagtg ggtattcgag cggcacaact cgtatcaaaa
      841 gtagttgtaa cttgatagga aagtgcttcg aaatcggcaa ctaaccatac cgccaaccga
      901 tatatacagc ttcgcatatt atcattcaca atcccaaagt ttgggggcta gagactgata
      961 cattgactca tattgaatgt attgaaatta caccaattta cacgcacata cgatttaaca
     1021 gacatttgca attataacct tttctccaaa taattcaaca tatttttcgg gggtaattgt
     1081 atgttcagga agtatttgtt tgggattaca attttcaaca aagtcaataa ttgtatagga
     1141 tagtcatatt gattaatttt tgttacaagt ataataaatt caaggggaaa gcaaataacc
     1201 gtgtgtattt tgacaatctt tgataaatat atttgtgctg tttaattaaa aaatctattt
     1261 cacgttccaa ttaaactcaa aataaaatgt aaatttgata atacaaatga aaattaatat
     1321 ataaaagata gcgaaacggt cctctgcaaa aaagatatat tataaaaagt tactgtaatg
     1381 tcaatctttt tgaatgattt aaaggtcaaa atttaataat attctctata tggaagtaat
     1441 tatttatatt gtgtttatat tgtgtgcgtt cattgagagt gtttttggct ttgcaggaac
     1501 aataacagca cttgcaataa tcggtatttt tgttgatatg aaaatagcaa tattattagg
     1561 actgtttttc ggtttaactc tttcaatata tatatattgt tatcagatat aaaatccttt
     1621 gattatacaa tttatttaaa ggttatttta cctgcaattc ccggtgtgat tataggttca
     1681 tatgtaattg aattagtttc tgttcatagt atgaaacttt tttttggtct ttttttagta
     1741 gtatttacca tatatactat ttttaaatta cagataccaa aaattttaga gaattttcta
     1801 ttaataataa atggtcttat acatggtttg ttaggagttg gtggacctat tcctgttgct
     1861 atttataaaa ataaaataaa taaatcaagt ttgagatata catttgcagt tgtttttata
     1921 acaatgaatg ttatgcgaat tgtacaatat tcaattcaaa aaacgtataa ttttaaacta
     1981 ttattaaatt attggctact tcctttttta ttattagttc ctgcattttt gggatataaa
     2041 attcatgtaa aaatttcaga aaaaaatttc agactagggt tatctattat tttattaata
     2101 atcgggttat attttttaat aagaagtttt atagcaatta cttgacatat tttgattctt
     2161 ggtacataaa aaaggtgttt ttaggaaagg gtttaatttt ataggaatgt atactcttgt
     2221 ctaaaaaaaa taatcttttg taatattttt cggcaaataa taaatacttg ttaataatgt
     2281 tattgccaaa taaagtggtt acgtgcaact ttgattataa tattccaatt aattttttat
     2341 acatgcatac ataatttgcg taatcaatct ttgattgaat aaggtttgtt gaagcttgtt
     2401 gccaaagtgt ttgagctgtt agcacatcgc ttacagggat ttttcctatt atataattgt
     2461 tttgtgtaat tctcagattt tcttgacttt gcataacggc tttttcatta attctaattt
     2521 gagtaagagc atgtttcatg tcttgttttg ttttttctaa ttcaagtttc attaattcac
     2581 tatttttttc taattcaagc tcctgatttt ttatgtctat ttgtgccgat gcaagttttt
     2641 gtttgccttc tgtccatgca aataaaggta tattaaccga tactatgaat tgaacatttg
     2701 aattggaata gtcttgagaa tttatttgaa ttcctccaat atgcgtgtaa ccacccttaa
     2761 gtcctatggt aggtaaatat gttgctcgtg tcatttttag ttcttgttct ttatatgcaa
     2821 ttttattttg taatatagaa tattcaggtc gtttttgtat agattcagtt ccttgtaagt
     2881 gtttccatat gagtgtgtca attggaatta tagtgtcata tattaatgga gtatacaaat
     2941 ccaggccaat tattcggcat aatgccatat t
//
LOCUS       DAOTOW010000230         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26072_length_2966_cov_3.113020, whole genome shotgun sequence.
ACCESSION   DAOTOW010000230 DAOTOW010000000
VERSION     DAOTOW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26072_length_2966_cov_3.113020"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>548
                     /locus_tag="P5243_05065"
     CDS             <1..>548
                     /locus_tag="P5243_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRS18851.1"
                     /translation="VLPTGQPLPFREQYRQITDSLNFSLRLFMRSGTLYLRYLKQINQ
                     MTSLIEQDLEKSIKNKELNKLLKMEKCLVYFITSIKANEIVLAKLRNSKKITTEINED
                     LLEDAFIENKQALEMAQIYSDIQSGMMDAFASVISNNLNVVMKKLTLISIILMIPTLI
                     ASIFGMNVINFMEDWPLALPSI"
     assembly_gap    549..558
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(685..1866)
                     /gene="purT"
                     /locus_tag="P5243_05070"
     CDS             complement(685..1866)
                     /gene="purT"
                     /locus_tag="P5243_05070"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRS18852.1"
                     /translation="MVTIGTPLSKSATKVMMLGSGELGKEVVIELQRYGVEVIAVDRY
                     ANAPAMQVAHKSYVISMLDAVALRTIIEQEKPQYIVPEIEAIATELLVDLEKEGYTVI
                     PSAKATHLTMNREGIRTLAAETLGLRTSPYRFAGNKEEYLKAVAEIGLPCVIKPVMSS
                     SGKGQSTVKTQEQIEAAWEYAREGARGSGGRVIIEGFVDFDYEITLLTISHKDGVSYC
                     APIGHRQEHGDYKESWQPQIMSAKALADAQYIAKEVVTALGGRGLFGVEFFIKGDIVY
                     FSELSPRPHDTGMVTMISQNLSEFALHVRAILGLPIPNIIQYAPAASSVIMVEGNSKA
                     VSFSGLEKALAEPDTQLRLFGKPEVQGERRMGVCLALGTDTDDARKKANNASRAIMYS
                     L"
     gene            complement(1958..2461)
                     /locus_tag="P5243_05075"
     CDS             complement(1958..2461)
                     /locus_tag="P5243_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRS18853.1"
                     /translation="MNTTTSILVLSDTHGYIDNDVLQHVKTVNQVWHAGDIGSLHVIE
                     TLEACVPLYAVYGNIDSHAIRMQTKEFLCFECNGLCVLLSHITGYPPKYAPGIIQKIK
                     EYTPSILVGGHSHICKVMNDANNSLLYINPGAIGKTGFHSVRTMIRFEITNAKPHNMS
                     VIELPRW"
     gene            2611..>2966
                     /locus_tag="P5243_05080"
     CDS             2611..>2966
                     /locus_tag="P5243_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18854.1"
                     /translation="MQNLRQQSIYIVIACVLAIACTTQKNTFTTRSYHTITSRYNIYF
                     NAREAYKSGMQNIEKSFKEPYNDILPVFYYSQKDILQSANGDMDRTLLKCTKLIKDHS
                     ITVKPAQKRPLTTQKQ"
BASE COUNT         1013 a          543 c          426 g          974 t
ORIGIN      
        1 gtattaccaa cagggcaacc cttacctttt agagaacaat accgtcaaat tacagatagt
       61 ttaaactttt cattacgatt gtttatgcgt tcgggaacat tgtatctgcg atatttgaaa
      121 caaattaatc aaatgacttc tcttattgaa caagacttag aaaaatccat taaaaacaaa
      181 gaattaaata aattgttaaa aatggaaaaa tgtttagttt actttattac ttctataaaa
      241 gcaaatgaaa tagtacttgc aaaactaaga aattcaaaaa aaattacgac agaaataaat
      301 gaagatttgc ttgaagacgc atttattgaa aacaaacaag ctttggaaat ggcacaaata
      361 tattccgata ttcaaagtgg aatgatggat gcgtttgctt cggtaatttc aaataattta
      421 aacgtagtaa tgaaaaaatt aacacttata tcaattatac ttatgattcc aacgttaatt
      481 gcaagtatat ttggtatgaa tgttatcaat tttatggaag attggcctct agcattaccc
      541 agtattatnn nnnnnnnnaa caggttctat cttattatcg gtatttggcg taatttggtt
      601 taaaaaacaa gaatggattt aatagtacaa ttataatata tcttacttaa ttaaatataa
      661 ttgtactgtt aaatacctaa aaatttataa cgaatacata attgctcggc ttgcattatt
      721 tgcttttttt cgagcgtcgt cggtatcggt tcctaatgct aaacaaactc ccattcgtcg
      781 ttctccttgc acttccggtt ttccgaacaa tcgtaattga gtatcgggtt cagctagagc
      841 tttttcaagt ccactaaaac ttacagcttt agaattacct tctaccatta ttacactgga
      901 tgctgcagga gcatattgaa taatatttgg aataggcaat cctaaaatag ctctcacatg
      961 caatgcaaac tccgataagt tttgagaaat catagttacc attccagtat catgaggacg
     1021 tggagataat tcactaaaat atacaatgtc acctttaata aaaaattcaa ccccgaacaa
     1081 gcccctgcca cctaaagctg taactacttc ttttgctatg tattgtgcat cggcaagagc
     1141 tttggctgac attatttgcg gctgccatga ttctttataa tctccatgtt cttgtctgtg
     1201 accaattggt gcacagtatg aaacaccatc tttatgactt attgtaagta atgtaatttc
     1261 ataatcaaaa tcaacaaaac cttcaatgat aactcgtcct cccgaccctc ttgctccttc
     1321 cctggcatat tcccatgctg cttctatttg ttcttgagtt tttacagtac tctgcccttt
     1381 tccggaagaa ctcattaccg gtttaattac acatggtaat cctatttctg ccactgcctt
     1441 gagatattct tctttgttac cggcaaaacg atatggagac gttcgcaaac ctaacgtttc
     1501 ggcagctaaa gtccgtattc cttcacgatt catagtcaaa tgagtagctt tggcagatgg
     1561 aataactgta tatccctctt tttctaaatc aactaacaat tctgttgcaa tagcttctat
     1621 ttcaggaact atatactgtg gtttttcttg ttctattatt gttcttaaag caactgcatc
     1681 gagcattgaa ataacatatg atttgtgtgc tacttgcata gcaggagcat ttgcatatct
     1741 atcaactgct ataacttcta caccataccg ttgcaattct attacaactt ctttacctaa
     1801 ttcacccgat cctaacatca ttactttagt tgcagacttg gaaagtggag ttcctatagt
     1861 taccatatct atatatattg atttattaca aaaataaaaa aagaccgatt gacaaaaaaa
     1921 aagctgcatt gcaacttact aacaacttat tgatacatta ccaacgaggt aactcaatta
     1981 ctgacatatt gtgaggtttt gcgtttgtta tttcaaatcg aatcatagtt cgcaccgaat
     2041 gaaaaccggt ttttccaatt gctcccggat ttatatatag taaggaattg ttggcatcgt
     2101 tcattacttt acaaatgtga gaatgtccac caacaagtat tgagggggta tattctttaa
     2161 ttttttgtat aataccggga gcatattttg gaggatatcc ggtaatatgc gatagtaaaa
     2221 cacataaacc attacattca aaacacaaaa attctttggt ttgcatacga atagcatgag
     2281 aatctatgtt cccatacact gcatacaatg gaacgcaagc ttctaaagtc tctataacgt
     2341 gcaacgatcc aatatcacca gcatgccata cctgatttac tgtttttaca tgctgtaaaa
     2401 catcattatc aatataccca tgagtatcag acaatactaa tattgaagtt gttgtattca
     2461 taaatgcaat atattatttt gtggaaataa atcagtacat aattcaaaaa aatctttact
     2521 caatgtaatg aatactaaaa atcaactttt tttttgctgc ctaaaaaata ttttcgatat
     2581 ttgacagttt tcttacaaaa attaacattt gtgcaaaatt tacgacaaca atcaatttat
     2641 atagtaatag cttgtgtttt ggcaatagct tgtacaacac aaaaaaatac atttaccaca
     2701 cgttcttacc atactataac gtctcgctac aacatatact tcaatgctcg cgaagcatac
     2761 aaaagtggaa tgcagaatat agagaaatca tttaaagaac catataatga catattaccg
     2821 gttttttatt attctcaaaa agacatttta caatcggcca atggtgacat ggatagaact
     2881 ctcttgaaat gtacaaaact tataaaagac cattctatta ccgtaaaacc agctcaaaaa
     2941 agaccattaa caactcaaaa acaaaa
//
LOCUS       DAOTOW010000231         2950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26247_length_2950_cov_2.729534, whole genome shotgun sequence.
ACCESSION   DAOTOW010000231 DAOTOW010000000
VERSION     DAOTOW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26247_length_2950_cov_2.729534"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="P5243_05085"
     CDS             <1..1399
                     /locus_tag="P5243_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRS18855.1"
                     /translation="TRIVLFSGNKWSNVDELRLAAIPQDNYIMGYYHSYEPWSFAGEG
                     QGTWGTPSDIAAMKARIAKGKDWVEKNNIPLIISEFGTDTLCDYNSRMLFYAYYVEEA
                     FKQNIAFAVWDDGGTFNVYRRNQQTWHDTKEILIHYSHVSPTQLELTQQDNSVKIHWL
                     NRMTADSVDIERRFTNSHFQTIARVANTDELYVDTDIIPEDFYYYRIKAYRNDSIFYS
                     YSQRILVAPNESQRNPYFSEPFVIPTSFQAEHFDKGGYGLTYYDVDFIQSHSNYRSEK
                     GVDIIQTQNQSYALIQNQKGEWYEYSVNVPIAAEYEISVYIGAITSGSFSIQSNGVIK
                     SKSFTATGSYTNVIPNSIRMQLQSGNQILRLNITKTGDFAIDSIAITGINSSLNTLEN
                     TDFRIIKHENNRFTVVSVNYSSHRQPISIYNVLGKQILQLQLEHGLCTFSLMHMPHGL
                     YLVKCGGIIRAIVVE"
     gene            complement(1410..2315)
                     /locus_tag="P5243_05090"
     CDS             complement(1410..2315)
                     /locus_tag="P5243_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS18856.1"
                     /translation="MVSFFLYLLLSIIAIDILSLITTYIPFIPTFSQKHSIILGLIVF
                     FTVGIIVLYGHIQALNTQIVHLPITIHKKVEGSQKIRILMASDLHFGAIIGERWEKKF
                     IQIVQQQHPDIVLLCGDIVDGDIAPVLRKKLGQHLQEITPPMGMYAITGNHEYIGGID
                     ATLQYLTSINITVLRDTIITLPNGIQIVCRNDIYSKKQRPLDSLLQHINHSKPIIVMN
                     HQPYNLHEAVTNNVDLHISGHTHHGQLWPFNYITRSIFELSWGYKHEKSTHLYVSSGF
                     GTWGPTVRIGNKPEVVVFDVTFSPM"
     gene            complement(2279..2536)
                     /locus_tag="P5243_05095"
     CDS             complement(2279..2536)
                     /locus_tag="P5243_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18857.1"
                     /translation="MKILVFILTVTTIYFAAHYYMFARIVHYFSLTPPVKNYVLWSII
                     GIASFFIVGMFLERAYSSIISEWIYKIGTTWLAFFSICYFL"
     gene            complement(2677..>2950)
                     /locus_tag="P5243_05100"
     CDS             complement(2677..>2950)
                     /locus_tag="P5243_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-structuring protein H-NS"
                     /protein_id="HRS18858.1"
                     /translation="MGRGAGNCTTELLVSFLKNPKYKLRPLLWAIEKHVRPLQKQIDW
                     GYFMPYMISGAMNDHPKSALKWMDSDRKEEYVAYYDQVRDSRQELE"
BASE COUNT         1019 a          423 c          507 g         1001 t
ORIGIN      
        1 tacaagaatt gttttatttt caggcaataa atggtcaaat gttgatgaat tacgtttagc
       61 agctattcct caagataatt atattatggg atattatcat tcatatgagc cgtggagttt
      121 tgcaggtgaa gggcagggga cttggggaac accttctgat attgcagcaa tgaaagcacg
      181 aattgctaaa ggcaaagatt gggtagaaaa aaataatata cctcttataa ttagtgagtt
      241 tggcactgat actttatgcg attataattc tcgtatgttg ttttatgcat attatgttga
      301 agaagctttt aagcaaaata ttgcatttgc agtttgggac gatggaggaa catttaatgt
      361 atatcgaaga aatcaacaaa cctggcatga taccaaagaa atacttattc actattctca
      421 tgtgagtcca acacaattag aacttacaca acaagataat agtgtgaaga tacattggct
      481 aaatagaatg acagcagata gtgttgatat tgaacgtcgg tttacaaatt ctcattttca
      541 aactatagct cgtgttgcaa atactgatga attgtatgta gacactgata taatacccga
      601 agatttttat tattatagaa tcaaagcata tagaaatgat tctatatttt attcatattc
      661 tcaaagaata ttggttgctc ccaatgaatc tcagagaaat ccatattttt ctgaaccatt
      721 tgttattcca acgtcatttc aggccgaaca ttttgataaa ggcggatatg gattgacata
      781 ttatgatgtt gactttatac aatcgcattc aaattatcgg agcgaaaaag gtgttgatat
      841 tatacaaaca caaaatcaat cgtatgcatt gattcaaaat caaaaaggag aatggtatga
      901 atattctgta aatgttccta ttgcagcaga atacgagata tctgtatata ttggtgctat
      961 tacaagtgga agtttttcaa tacaatctaa tggggtaatt aaatcaaaat ctttcactgc
     1021 cacaggctca tataccaatg taattccaaa ttctatacga atgcaattac aaagcggaaa
     1081 tcaaatttta cgattaaata ttactaaaac aggagatttt gcaattgaca gtattgcaat
     1141 taccggtatt aattcttctc taaatacttt ggaaaatact gattttagaa ttataaaaca
     1201 tgaaaataat aggtttactg tagtttctgt taattattct tctcacaggc aacctattag
     1261 tatatataat gtgttgggaa aacaaatttt gcaattacaa ttggagcatg gtctgtgcac
     1321 attttcattg atgcatatgc ctcatggatt gtatttagta aaatgcggag gcattattcg
     1381 ggcaattgtt gtggagtaat gagtagctgt tacataggtg aaaatgtaac atcaaaaaca
     1441 acaacttcag gtttattacc aattcgtact gttggtcccc aagtgccaaa tcccgacgaa
     1501 acatataaat gagttgattt ttcatgtttg tatccccaac ttaattcaaa tattgaccgg
     1561 gtaatataat taaatggcca taattgtccg tgatgagtat ggccgcttat atgtaaatca
     1621 acattattag ttacagcttc gtgtaagtta tatggttggt gattcattac aattataggt
     1681 ttagaatgat taatgtgttg tagtaatgaa tcaagggggc gttgtttttt gctgtaaata
     1741 tcattgcgac aaacaatttg aattccgtta ggtaatgtga ttatggtatc acgtaatact
     1801 gttatattga tacttgtaag atattgtaat gtagcatcaa ttcctccaat atattcatga
     1861 tttccggtaa ttgcatacat tcccatagga ggagtaattt cttgtagatg ttgtcccaat
     1921 ttttttcgta atacaggtgc tatatcacca tcaactatat cgccacataa taatacaata
     1981 tcaggatgtt gttgctgtac tatctgtata aattttttct cccaacgttc gcctataata
     2041 gcaccaaaat ggaggtcaga tgccataaga attcttattt tttgtgaacc ttctactttt
     2101 ttgtgaatag taattggtaa gtgtactatc tgtgtgttaa gagcttgaat atgaccgtat
     2161 aaaacaataa taccaactgt gaaaaataca attaagccca atataataga atgtttttga
     2221 gaaaatgtag gaataaaggg aatatatgtt gtaataagac tgagaatatc tatagcaatt
     2281 atagaaagta acaaatagag aaaaaagcta accacgttgt accaatttta tatatccatt
     2341 cactaattat tgatgaatag gcacgttcta aaaacatacc tacaataaaa aaagaagcaa
     2401 tacctatgat gctccacaac acgtaatttt ttacaggagg tgttaatgaa aaataatgaa
     2461 ctatgcgagc aaacatatag taatgagctg caaagtatat tgtagtaacg gtgagtataa
     2521 aaactagaat tttcaaagtt ttaaagtatt atatattatg tatttgatgt tgaaatgata
     2581 tccaccttac ataggtatta atataaaatg tgtttttttt taataaaaaa aggtgtttgt
     2641 aaaatcaaac acctttaatt atatctattt tttagattat tcgagttctt gtcgtgaatc
     2701 tctaacttga tcatagtatg caacgtattc ttcttttctg tctgaatcca tccattttaa
     2761 agcacttttg gggtggtcat tcatagcacc ggaaatcata tatggcataa agtatcccca
     2821 atcaatttgt ttttgaagag gacgcacatg tttttcaata gcccataata aaggacgaag
     2881 tttatatttt ggatttttaa ggaatgatac taataattct gtagtacaat ttccggcacc
     2941 gcgtcccata
//
LOCUS       DAOTOW010000232         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26271_length_2948_cov_2.864155, whole genome shotgun sequence.
ACCESSION   DAOTOW010000232 DAOTOW010000000
VERSION     DAOTOW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26271_length_2948_cov_2.864155"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="P5243_05105"
     CDS             <1..1202
                     /locus_tag="P5243_05105"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRS18859.1"
                     /translation="LQICKSENFTNIVCSIKASNTRIMVYATRLLVQKMKEENLLFPI
                     HLGVTEAGAGEDGRIKSAVGIGTLLLEGIGDTIRVSLTEAPEHEIPVCKTIVDYRNRV
                     FADNLHTVLPFDIRPNPFTYTKFISHSVYGIGGNAAPIVIANSMNIGADWFFHDNSVL
                     TKEDGDYDLEYILFDDLQAKHIGKHIVIEIEVEQIHHLHTKHIPEDCIIVLRYGGKKL
                     QYWIQKLYAELHNAHCNPPIILRLQYDIESYNTFEIQAAMDAGSVFIDGFCDGLWIEN
                     QHVSESECVQLAFSILQATRARFTKTEFISCPSCGRTLFNIEKTLAEVKAKTSHLQGL
                     KIAVMGCIVNGPGEMADADYGYVGAGKGLVHVYCKQQIIRKNIPEQEAVESLIEVIKE
                     NGDWKEH"
     gene            1219..1671
                     /locus_tag="P5243_05110"
     CDS             1219..1671
                     /locus_tag="P5243_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18860.1"
                     /translation="MTVKYLSIISLICIICFQTSCKQSTYTKVFTFEQHKVPNKPLEF
                     IFENPHANKTFDVTLKLTHTPHIKHNQFVFDFAIGNNFSTTLGVVLRNRDGNFKGVKQ
                     GDSIVLKQSLLQNQLLPQGRNTFLITPAMNVDTVYNISAIELQIKEAE"
     gene            complement(1716..2144)
                     /locus_tag="P5243_05115"
     CDS             complement(1716..2144)
                     /locus_tag="P5243_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS18861.1"
                     /translation="MKRFVITLSIIVGFLISGYAQSPNWNDPSEKKLDISEIKGPVMT
                     WVKDSYDFGSIPQGVPVHVTFEFTNTGNAPLIISKVEPACNCTAADYPKTPIMPGQKG
                     SIKITFDAASTGKFSKTAIVTSNAKPTEKMLVFTGTVVKK"
     gene            2518..2943
                     /gene="ssb"
                     /locus_tag="P5243_05120"
     CDS             2518..2943
                     /gene="ssb"
                     /locus_tag="P5243_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRS18862.1"
                     /translation="MINKVILVGNVGADPEIRFISEGVITGRIKLATSETYLDKNKER
                     VTKTEWHNVVVWNSLAKTAETYVKKGMQLYIEGKISTRSYEKDGETKYVTEIIASEMK
                     FLGKKAESSTQNDTKTNVAAEPEPMPIDITPEPSDDLPF"
BASE COUNT         1056 a          464 c          473 g          955 t
ORIGIN      
        1 ttttacaaat ttgcaaatca gaaaatttta caaacatagt atgctctata aaagcaagta
       61 atacccgtat aatggtatat gctacccgac tgcttgttca aaaaatgaaa gaagaaaacc
      121 tgttgtttcc aattcattta ggagttaccg aagccggcgc aggtgaagat ggcagaatta
      181 aatctgcggt tggaatcgga accttattgc ttgaaggtat tggagatact attcgagttt
      241 cgctaacaga agctccggaa catgaaatac cggtttgtaa aacaatagta gattatcgaa
      301 atcgtgtgtt tgccgataat ttacatacag ttttaccatt tgatataaga ccaaaccctt
      361 ttacatatac caaatttatt tcacacagtg tgtatggcat tggaggtaat gctgctccta
      421 ttgtaatagc aaattcaatg aatataggtg ccgattggtt ctttcatgac aatagtgtac
      481 ttacaaaaga agatggtgat tatgatttag aatacatctt atttgatgac ttgcaagcga
      541 aacacatagg caaacatatt gttatagaaa ttgaagtaga acaaatacat catttacaca
      601 ctaaacatat tcccgaagat tgtataatag tactacgata tggcggcaaa aagctacaat
      661 attggattca aaaattatat gccgaattac acaatgctca ttgcaatcca ccaataatat
      721 tgcgattgca atacgatata gaatcataca atacgtttga aattcaagct gcaatggatg
      781 ccggatcggt ttttatcgat ggcttttgtg atggtttatg gattgaaaat caacatgtgt
      841 cagaatcgga atgtgtacaa ttagcctttt caattctgca agctacacga gctcgtttta
      901 caaaaacaga atttatttct tgtccatcat gtggcagaac cctgttcaat attgaaaaaa
      961 ctttagctga agtaaaagca aaaacatctc atttacaagg ccttaaaata gcagtaatgg
     1021 gatgcattgt aaatggcccc ggagaaatgg ccgatgctga ttatggatat gtaggcgcag
     1081 gtaaaggatt agttcatgta tattgcaaac aacaaattat tcgcaaaaac attccggaac
     1141 aagaagctgt tgaatcttta atagaagtaa taaaagaaaa tggcgattgg aaagaacatt
     1201 aatataacct atatttctat gactgtaaaa tatttaagta ttatatctct tatatgtatt
     1261 atttgctttc aaacatcttg caaacaaagc acttatacaa aagtattcac atttgaacaa
     1321 cataaagttc ccaacaaacc tttagaattt atttttgaaa atcctcatgc aaataaaaca
     1381 tttgatgtta cgctaaaact tacccatact cctcacataa aacataatca gtttgtattt
     1441 gattttgcta taggaaacaa tttttcaact actcttggtg ttgtattgcg taatcgcgat
     1501 ggtaatttta aaggtgtaaa acaaggcgat tctattgttt tgaaacaatc tttattacaa
     1561 aatcaattat tgccacaagg tagaaatact tttttaatta ctcctgccat gaatgttgat
     1621 acggtatata acatttctgc aattgaacta caaataaaag aagccgaata aaatatttca
     1681 tttattcggc ttctttaaat tgtatatttt cttatttact ttttaaccac agtacctgta
     1741 aaaactaaca ttttttcggt tggtttagca ttagatgtta ctatggcagt ttttgaaaat
     1801 ttaccggtag aagcagcatc aaatgtaatt tttatactgc ctttttgtcc cggcattata
     1861 ggtgtttttg gataatccgc tgcagtacaa ttacatgcag gttctacttt tgaaattatt
     1921 aacggtgcat tacctgtatt tgtaaattca aatgtaacat gtaccggcac tccttgagga
     1981 atagaaccaa aatcatacga atcttttacc catgtcatta caggaccttt aatctcggaa
     2041 atatctaatt ttttttcgga aggatcattc caatttggag actgtgcata tccactaatt
     2101 agaaaaccta caataatcga tagtgttatt acaaaacgtt tcatagttgt attttttata
     2161 ttagacatgt aacacttatt tttggttgca tacattacta aaaaaatatg ctatgcaatt
     2221 tcacgatgtg ttttcaaaaa tcttgccaat acaagcactg tataaacttt atttttaaaa
     2281 attattatta aatatgtttc agcccatagt aattagtaaa acttcaactc ttattgtata
     2341 aattaggtaa taccaatatg tattaaaata taatcaatct acattaattg tattagttgg
     2401 agctatatca aaacttatat ttttacgcta aattaattgt tcatatttgg ttttttgttg
     2461 cattccaaat tttttatgta attttagggc attgtttaac taaaaaaaga gaaagacatg
     2521 attaacaaag taattttagt aggaaatgta ggggcagatc ctgaaatccg tttcattagt
     2581 gaaggtgtta taaccggtag aattaaactt gctaccagcg aaacatacct tgacaaaaac
     2641 aaagaaagag ttaccaaaac tgaatggcac aatgttgtag tatggaactc actggcaaaa
     2701 acagctgaaa cctatgtaaa aaaaggtatg caattgtaca ttgaaggtaa aataagtacc
     2761 cgttcgtatg aaaaagacgg agaaactaaa tatgttactg aaataattgc aagcgaaatg
     2821 aaatttttag gcaaaaaagc agaaagctct actcaaaacg acacaaaaac aaatgtagct
     2881 gcagaacctg aacctatgcc tattgacata actccggaac cttctgatga cctaccattt
     2941 taatatat
//
LOCUS       DAOTOW010000233         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26346_length_2942_cov_3.374784, whole genome shotgun sequence.
ACCESSION   DAOTOW010000233 DAOTOW010000000
VERSION     DAOTOW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26346_length_2942_cov_3.374784"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="P5243_05125"
     CDS             <1..463
                     /locus_tag="P5243_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRS18863.1"
                     /translation="TGIEIPKPSNFLVRIGTSIHTLFEAVGGIPQNTEKIISGGPMMG
                     KALIHSHMPVSKGTSGVIAVPYNLAHRDEPFNCIRCARCVQVCPMGLQPYLLQLYSEQ
                     RMYEQLETNFVLDCIECGSCSYICPSSRPLLDYIRLGKNKVSQIIRKRKQK"
     gene            465..1451
                     /locus_tag="P5243_05130"
     CDS             465..1451
                     /locus_tag="P5243_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRS18864.1"
                     /translation="MYTVSASPHIHAKQNVNRIMYDVIIALLPAFGVSILFFGMSALY
                     TTLIAILSCIVIEFLIHKFLFKKQDFSIKDGSAVITGMLLAFNVPSSIPWWIIIIGSF
                     VAIGIAKMSFGGLGNNIFNPALVGRVFLLISFPVDMTLWPKTTLNQSVDGLTGPTPLA
                     LLKEGIVSGKNIMQIKGLPNYQDMFLGNIGGSLGEISALAIIIGGIYLLFRKVITWHI
                     PVFVLLSMFVFSGIFYLLNPYMYINPLFHLLSGGAMLGAWFMATDMATSPMTIKGQII
                     FAVGIGIITMIIRMFGAYPEGISFAILLMNAVVPLLNKISPSRYGSSLSIKK"
     gene            1457..2068
                     /locus_tag="P5243_05135"
     CDS             1457..2068
                     /locus_tag="P5243_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HRS18865.1"
                     /translation="MAKQAASSLRNMILSLFIITTISGASLSYIYKITAKGIEQSRQQ
                     KKIEAITQVLPPFTNNPLKEMYKMPLFENDSLEVYPARKNDTLIGIAIASKTNSGFAG
                     EIKIMVGFLPSGVIYKTKVIEHHETPGLGDKMEQPEFYKQFEQFNFHEKINTVKKNGG
                     DIDAITAATISSRAYCNALQNAYSIFQKINQQWSADSQTGATK"
     gene            2079..2660
                     /gene="rsxE"
                     /locus_tag="P5243_05140"
     CDS             2079..2660
                     /gene="rsxE"
                     /locus_tag="P5243_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HRS18866.1"
                     /translation="MTNVSLITKGIIKENPVFVLLLGMCPTLAVTGSGINGFAMGAAT
                     MFVLILSNIVISGIKSTIPSKVRIPAYIVVIATFVTIVQLLMEAYTPSLHKQLGLFIP
                     LIVVNCLILGRAEAFASKNNMFHSFLDGLGMGLGLTILGLVRELLGAGTLFGINITGM
                     QHPKMMLVFILPPGAFIVLAYLVSLNRMVQKNK"
     gene            2667..>2942
                     /locus_tag="P5243_05145"
     CDS             2667..>2942
                     /locus_tag="P5243_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRS18867.1"
                     /translation="MTYILIIISAVFVNNIVLSQFLGICPFIGVSNKVKTAIGMSAAV
                     LFVMVLATMVTYMLFFYILKPLQLQFLNTITFIVIIASLVQMVEIIIK"
BASE COUNT          981 a          401 c          519 g         1041 t
ORIGIN      
        1 aacaggtata gaaattccta aaccatcaaa ttttttagta cgaataggaa cttctattca
       61 tacccttttt gaagcagtag gaggaatacc tcaaaataca gaaaaaataa tttcaggcgg
      121 gccaatgatg ggtaaggctt taattcattc tcacatgccc gtttcaaaag ggacttcggg
      181 agtaatagct gttccttata atttagctca tcgcgatgaa cctttcaatt gtattcgatg
      241 cgcacgctgt gtgcaagtgt gccctatggg tttacaacca tatttgttac aattgtattc
      301 agagcaaaga atgtatgagc aattagaaac aaattttgtg ttagattgca tagaatgtgg
      361 ttcttgtagt tatatttgtc cttcatctcg gccgcttctg gattatataa gattagggaa
      421 aaataaagtt tcacaaatta ttagaaaacg taaacaaaaa tagtatgtat acagtttcag
      481 cttcacctca tattcatgca aaacaaaatg tgaatcgcat aatgtatgat gtaattatag
      541 ccttgctacc tgcgtttggt gtgtcaattc tgttttttgg aatgtctgca ttatatacaa
      601 cactaatagc tattttatct tgtattgtaa tagagttttt aattcataaa tttttattta
      661 aaaagcaaga tttttctatt aaagatggat ctgctgtaat tacaggaatg ttgttagcat
      721 ttaatgtgcc ttcaagtata ccttggtgga taattattat tggaagtttt gttgctatag
      781 gtattgcaaa aatgtctttc ggaggtttag gtaataatat atttaatccg gctttggttg
      841 gaagggtctt tttgttgatt tcattcccgg ttgatatgac tctttggcca aaaaccacat
      901 taaatcaaag tgttgatgga ttaaccggac caactccatt ggcgttgctt aaagagggaa
      961 ttgtgagtgg taaaaatatt atgcaaataa aagggttacc aaactatcaa gatatgtttt
     1021 taggtaatat aggaggaagt ttgggagaaa tatcagcatt agcaattatt ataggtggaa
     1081 tttatttgtt gtttcgcaag gtaattacat ggcatatacc ggtatttgta ttgttaagta
     1141 tgtttgtatt ttcaggtata ttttatcttc taaatcctta tatgtatatt aatcctttgt
     1201 ttcatttgtt atctggagga gcaatgcttg gtgcatggtt tatggcaacc gatatggcaa
     1261 catcacctat gactattaaa ggacaaatta tttttgctgt tggaataggt attattacaa
     1321 tgataattcg aatgtttgga gcttatcctg aaggaatatc atttgcaatt ttattaatga
     1381 atgcagttgt tccgttgtta aataaaattt cgccatcacg ctatggtagt agtttgagta
     1441 ttaaaaaata aatcttatgg caaagcaagc agcatcttca ctgagaaata tgattctttc
     1501 actatttatc attacaacta tttcaggtgc atcattatca tatatttata aaattacagc
     1561 aaagggaatt gaacaatcac gtcagcaaaa aaaaatagaa gctataactc aagtattgcc
     1621 accttttacc aacaatcccc ttaaggaaat gtataaaatg ccgttattcg agaatgatag
     1681 tttagaggta tatcctgctc gtaaaaatga tacattaata ggaattgcaa tagcttcaaa
     1741 aacaaattcg ggatttgccg gagaaataaa aataatggta ggttttttac caagtggtgt
     1801 aatttataaa actaaagtta ttgaacatca tgaaactcct ggtttaggtg ataaaatgga
     1861 acaaccagaa ttttataagc aatttgaaca atttaatttt catgaaaaaa taaataccgt
     1921 gaaaaaaaat ggtggtgata ttgatgcaat aacagcagca acaattagtt ctcgagcata
     1981 ttgtaatgcc ttacagaatg catatagtat atttcagaaa ataaatcaac aatggtctgc
     2041 cgattcacaa acgggggcta ctaaataaat taaaaattat gacaaatgta tcactcatta
     2101 caaaaggaat aattaaagaa aatccggttt ttgtcttatt gcttggtatg tgtcctacgt
     2161 tagctgtaac aggttccggt attaatggtt ttgctatggg ggcagccaca atgtttgtgt
     2221 taatattatc aaatattgta atatcaggaa ttaaatctac tattccttca aaagttagaa
     2281 ttccagcata tattgttgta atagcaactt ttgttactat agtccaactc ctaatggaag
     2341 catatactcc ctcactgcat aaacaattag gattatttat tcccttaata gttgttaatt
     2401 gtcttatttt aggaagagca gaagcctttg catcaaaaaa taatatgttt cattcatttt
     2461 tagatggttt aggtatggga ttaggattaa caatattagg tttagttcga gagttattag
     2521 gtgccggtac tttatttggt attaatataa ccggaatgca gcatcctaaa atgatgctgg
     2581 tttttatatt accaccggga gcttttattg tattggcata tttggtttca ttaaacagaa
     2641 tggttcaaaa aaataaataa tattatatga catacatttt aatcattata tcagcagtat
     2701 ttgtaaataa tattgtatta agtcaatttc ttggaatttg tccgtttata ggtgtttcaa
     2761 ataaagtaaa aacagcaatt ggtatgtctg ctgcggtttt atttgttatg gtacttgcaa
     2821 ctatggtaac atatatgttg tttttttata tacttaagcc attacaactg caatttttaa
     2881 ataccattac atttattgtt attatagcct cattagtgca aatggtagaa ataattatta
     2941 aa
//
LOCUS       DAOTOW010000234         2941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26358_length_2941_cov_2.682606, whole genome shotgun sequence.
ACCESSION   DAOTOW010000234 DAOTOW010000000
VERSION     DAOTOW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26358_length_2941_cov_2.682606"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="P5243_05150"
     CDS             complement(<1..1414)
                     /locus_tag="P5243_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRS18868.1"
                     /translation="MKPIQCIVICLFVSSFCFFSAIGQTITVQAPKVVEEGEFVHITY
                     SINVRPGDNFKVSTVDGLQASGRPQTSTSNSIQIINGKTTSEYTYSETHQFRAIRKGV
                     FKIPIASVVIDGKTISSKTQEIEVVAASSNNSAQRQSSGAAAQQAYQDPTPSSAGTDV
                     FARVYTNKQTAYIGEPIYVSIKLYTQKNFNINTHTFPEFTGFYKKIIEQPQELKFERE
                     VIDGKEYYSVLFQRLVLFPQKSGKLTITPFKLDCNVITQVGNSFWNMYNKQERRIIES
                     KPVYIQVLPFPQGKTSDFNGAVGEFSITAKTDVQTIPANEAITYKFTIQGIGNLALLQ
                     KPTIDFPKDFEVYEPKIIDSYTTTTMGDKGSKTFEYVVIPRHEGNFTIPQVVMQVFNT
                     ETKTFKTITADAIQIAVTKGAVTNQVTTNFGTNKEQIQYSADDFRYIYTDDLQLHSIS
                     SYVIINPWFNGLFVILVLGAA"
     gene            complement(1427..1882)
                     /locus_tag="P5243_05155"
     CDS             complement(1427..1882)
                     /locus_tag="P5243_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18869.1"
                     /translation="MLLANRGLEVYENDEKLTKLIELMKENKNSSSQNQQNQKNQNTN
                     QDNNSQNNSQNGNQDNKQQNNDTNSKNGSNQNSNQNKQSGEQQQKQGEQSKEQSGEKS
                     KEQYVKGQISEVEAEMMLRAIENNERAIQLKLQKQKQEQQRKQVIEKNW"
     gene            complement(2008..2676)
                     /locus_tag="P5243_05160"
     CDS             complement(2008..2676)
                     /locus_tag="P5243_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS18870.1"
                     /translation="MNRVVFILFCFVCFHSYAQRDVRMYIDKGNALYAKQDFRSAAVT
                     YKHACMLDSVNFDAWYNFGNALYKLEKYILAGDAYNKALQLTSDSKLQAQTLYNVGNA
                     YLKQDDFENAIEAYTKSLLLNPAQPDCRYNLSYALKLQELQQKDKNNSKNNQNKQKNP
                     QISEFAQQCKAQADTLVAQYKFSEAYDVMKQGLAKDKTVAEYSDFIQRLQKISEILEN
                     KSKP"
     gene            complement(2695..>2941)
                     /locus_tag="P5243_05165"
     CDS             complement(2695..>2941)
                     /locus_tag="P5243_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRS18871.1"
                     /translation="QNIKKFCDMLQALVGVVPGIELLEVGSKQAASPADNFDVVLISE
                     FKTWADLQAYAVHPEHQKVVAFAKEVVSSRAAVDYEF"
BASE COUNT          852 a          502 c          435 g         1152 t
ORIGIN      
        1 cagccgctcc taatactaat ataacaaata atccattaaa ccaaggattt attattacat
       61 atgatgatat tgaatgcaac tgcaaatcat cggtatatat atatctaaaa tcatcggcag
      121 aatattgtat ttgttctttg tttgttccaa aatttgttgt tacttgatta gtaacagcac
      181 ctttggttac agctatttgt atggcatctg cagtaattgt tttaaacgtt ttggtttctg
      241 tattgaatac ctgcattaca acttgaggaa ttgtaaaatt tccttcatga cgaggaatta
      301 ccacatattc aaatgttttg gaacctttat cccccatagt tgtagttgta tacgaatcta
      361 ttattttagg ttcatacact tcaaaatctt taggaaaatc aattgttggt ttttgtaaca
      421 aagctaaatt accaattccc tgaatagtaa acttatacgt aatagcttca tttgccggaa
      481 tagtttgcac atcggttttt gccgttattg aaaattcccc cacagcacca ttaaaatcag
      541 aggttttacc ttgaggaaat ggcagcactt gtatatatac aggtttactc tctattattc
      601 ttcgttcttg cttattgtac atattccaaa aactgtttcc tacttgagtt ataacattac
      661 aatcaagttt aaagggtgta attgtaagtt ttcctgattt ttgaggaaaa agcactaatc
      721 gttgaaacaa taccgaataa tattctttgc catcaataac ctcacgttca aatttaagtt
      781 cttgcggctg ttcgattatc tttttataaa aaccggtaaa ttcggggaat gtatgagtat
      841 ttatattaaa atttttctgt gtatataatt taattgatac ataaataggt tcaccaatat
      901 aagcagtttg tttattagta tatacacgag caaacacatc ggttcctgcc gatgaaggtg
      961 ttgggtcttg atatgcttgt tgagcagctg ctcccgacga ttgtctttgt gcagaattat
     1021 ttgaagaagc tgcaactact tcaatttctt gtgtttttga tgatattgtt ttaccatcta
     1081 taactacact ggcaataggt attttaaaaa cccctttgcg tatagctcta aattgatgag
     1141 tttcactata tgtatattcc gatgtagtct ttccgtttat tatttgaata ctattactgg
     1201 tacttgtttg tggtcttccg gaggcttgca atccgtcaac ggtagatact ttaaaattat
     1261 cacccggacg tacattgata gaatacgtaa tatgaacaaa ttcaccttct tcaactactt
     1321 taggtgcctg taccgtaatt gtttgtccta ttgccgaaaa gaaacaaaaa ctacttacaa
     1381 ataaacaaat tactatacat tgtatgggtt tcatagttgt acttttttac caatttttct
     1441 caattacctg ttttcgttgt tgttcttgtt tttgtttttg aagttttaat tgaattgctc
     1501 gctcattgtt ttcaatagct cgcaacatca tttctgcttc aacttcagat atttgccctt
     1561 ttacatattg ttcttttgat ttttcaccgg attgttcttt tgattgttct ccctgctttt
     1621 gctgttgttc tcctgactgt ttattttgat ttgagttttg attactgcca ttttttgagt
     1681 ttgtgtcatt attttgctgc ttattatctt gattcccatt ttgagaatta ttttgagaat
     1741 tattatcttg attagtattt tgattttttt ggttttgctg gttttgactg gaagaatttt
     1801 tattttcttt catcaattca ataagttttg taagtttttc atcattttca tatacttcca
     1861 accctctatt tgcgagaagc aatgcctctt tttgttttcc ttctctttac aatttcgtcg
     1921 acatattaca atagtcttga acggtttttt gtgcttgaac cattagcaca caaactatga
     1981 aaacaacaat agaataaaaa cgttttttca tggttttgac ttattttcta atatttctga
     2041 aattttttgt aatcgttgta taaagtcact gtattctgca acagttttat ctttggccaa
     2101 cccttgtttc ataacatcat atgcttcact aaatttatat tgagcaacca aagtatcggc
     2161 ttgcgcttta cattgctgag caaattctga aatctgtgga tttttttgtt tattttgatt
     2221 atttttagaa ttattcttat ctttttgttg taattcttgt aatttaaggg catacgataa
     2281 attatatcta caatcaggtt gtgcaggatt tagtaacaat gattttgtat aggcttcaat
     2341 agcattttca aaatcatctt gttttagata tgcatttcca acattataca aggtttgagc
     2401 ttgcaactta gaatcagaag taagttgcaa tgctttatta tatgcatctc ctgccagaat
     2461 atatttttct aatttataca atgcattacc gaaattgtac catgcatcaa aatttacaga
     2521 atcgagcata caagcatgtt tgtaagttac tgctgcacta cgaaaatctt gttttgcata
     2581 caatgcatta cccttatcaa tatacattcg cacatcacgt tgagcatacg aatgaaagca
     2641 aacaaaacag aataatatga atacaacccg attcataatt cttaagtttt ttaattaaaa
     2701 ttcataatca actgctgctc ttgaacttac aacttcttta gcaaatgcta ccaccttttg
     2761 atgttccgga tgaacggcat acgcttgtaa atctgcccat gttttaaact ctgatatcaa
     2821 aactacatca aagttatcag caggagaagc agcttgtttt gaacctactt caagcaattc
     2881 tattccgggc acaacaccaa ccaatgcttg caacatatca caaaattttt taatattttg
     2941 t
//
LOCUS       DAOTOW010000235         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26563_length_2923_cov_4.822176, whole genome shotgun sequence.
ACCESSION   DAOTOW010000235 DAOTOW010000000
VERSION     DAOTOW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26563_length_2923_cov_4.822176"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="P5243_05170"
     CDS             complement(<1..1627)
                     /locus_tag="P5243_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS18872.1"
                     /translation="MKAVNIQKRISELEHENNVLKQTIAGYEQKLSQKCSNEEFLKAE
                     SVNKDLELAKQELEQYKSHLEEVIKERTVELKNSEMRFLTLSDSLSGGAIFEITLTKE
                     DIPWIGYMSKTTSSLLEFDLNLMRDNLGAFYERIYPDDLPIFNQAFTLSKETKCEFNV
                     EVRFITPSNKIKWLHFKANAIEQTNNYTVWVGIIIDTTQKNIFQQEIQEREAILNAII
                     ENIPYDFWARDAQLRCFLQNSASKRLWGNIIGTLPEELPVNETDKNLLQQLMFRVKQG
                     EIVNEEIQMTDKHGSLYDFQTLIAPILLQGKIQGVLGLNIDITDRKQVEKALIQNEEK
                     FRNIFDSSTDAIVIHTIDGIIIEMNKEFQKLLQLTSVNVVLDLHTLIPIVDFELFKTN
                     IINNCNCNATENVLFETELELKNNQKIPIEIKSKEIDYLGSKAILSLIQNTTYRKKFE
                     RQLVTAIVETEERERARFASDLHDDLGPILSSMKMLTGLLVDTKDFDTIKNISNQLYE
                     LVSESIRSVRETSNSISPHILKNYGIIAATRNII"
     gene            complement(1632..2468)
                     /locus_tag="P5243_05175"
     CDS             complement(1632..2468)
                     /locus_tag="P5243_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18873.1"
                     /translation="MIVIADSGSTKTDWCIVHEEAKFMFHTKGLNPYFVTTQNIVEEL
                     QNGYSYTLPASIVTQVYFYGAGCKSKESQIIVRRALEQFFTNAQIVIDSDLVGAAIAL
                     YGNKPGVVAILGTGMSIAYWNGNQIQDMMPSLGYILGDEGSGANIGKSFIQLLYYNKL
                     SQSISDEWNVTFQFTLAQVLEQIYKKPYPNRFLASFVPFVYKHKNDSTIQAMLEQIFL
                     DFALQVETIANKYDCKSVSCVGSVAYFFKDFVYLALQKKSIELLHTESSPINLLVNYY
                     RT"
     gene            complement(2475..2774)
                     /locus_tag="P5243_05180"
     CDS             complement(2475..2774)
                     /locus_tag="P5243_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRS18874.1"
                     /translation="MILINKTLSFSESIQSDAILWMKDTYIPLMHACDLIQNVLLFKI
                     ETQQEADDCFALQVFFENKEQYELYCGKYQDDFDAILLLKFKNSIGIYKTMLTQI"
BASE COUNT          984 a          462 c          359 g         1118 t
ORIGIN      
        1 gaattatatt tcttgttgct gcaataatac cataattttt taatatatga ggactaattg
       61 agttagaagt ttcacgaaca cttcgaattg attctgaaac taattcatac aattggtttg
      121 aaatattttt aatcgtatca aaatctttag tatctacaag taatccggta agcattttca
      181 tacttgaaag tattggtccc aaatcatcat gtaaatctga agcaaaacgt gcacgttctc
      241 tctcttcggt ttcaactata gctgttacca attgacgttc aaatttttta cgatatgttg
      301 tattctgaat aagagaaaga attgctttac tacctaaata gtcaatttct tttgatttaa
      361 tttcaatagg tattttttga ttatttttta attcaagttc tgtttcaaac aaaacatttt
      421 ctgttgcatt acaattacaa ttatttataa tatttgtttt aaataattca aaatcaacaa
      481 tgggaataag agtatgtaaa tcaagaacaa cattaacaga tgtaagttga agtaattttt
      541 gaaactcttt attcatttct ataattatac catcaatagt atgaattaca atagcatctg
      601 tactactatc aaatatattt ctaaattttt cttcgttttg gattaacgct ttttcaactt
      661 gttttctatc tgttatatca atatttaatc ctaaaactcc ttgtatttta ccttgtaata
      721 aaataggagc tatcagtgtt tgaaaatcat acaaagaacc atgtttatct gtcatttgta
      781 tttcttcgtt tacaatttct ccttgtttta ctcgaaacat caattgttgt aaaagatttt
      841 tatcagtctc attaacaggc aattcttcag gtaacgttcc aattatattt ccccataaac
      901 gttttgatgc actattttgc aaaaaacaac gcaattgagc atcgcgagcc caaaaatcat
      961 atggtatatt ttctattatt gcatttaata tagcttcacg ttcttgaatt tcttgttgaa
     1021 aaatattttt ttgagtagta tctataatta tacctaccca aactgtataa ttattggttt
     1081 gttcaattgc atttgcttta aaatgaagcc attttatttt atttgaggga gttataaaac
     1141 gaacttcaac attaaattca cactttgttt ctttacttaa tgtaaaagct tgattaaaaa
     1201 taggcaaatc atcaggataa attctttcat aaaaggctcc taaattatcg cgcattaaat
     1261 taagatcgaa ttctaataat gaacttgtgg tcttactcat gtatcctatc catggaatat
     1321 cctctttggt aagtgtaatt tcaaatattg ctccaccact taaactgtca ctcaatgtta
     1381 aaaaacgcat ttcactattt ttgagttcaa cagtacgttc ttttataact tcctcaagat
     1441 gacttttata ttgttctaat tcttgttttg ctaattctaa atctttattt acactttcag
     1501 cctttaaaaa ttcttcatta ctgcattttt gcgataattt ttgttcatat ccggcaatag
     1561 tttgcttaag aacattgttt tcatgctcca attccgaaat tcgtttttga atatttaccg
     1621 ccttcaaaat attatgttct atagtaatta accaataaat taataggcga cgactctgta
     1681 tgaagtagct ctattgattt tttttgtaat gccaaatata caaaatcttt aaagaaatat
     1741 gcaacagacc caacacaaga aactgatttg cagtcatatt tatttgcaat tgtttcaact
     1801 tgtaaagcaa aatccagaaa aatttgttct aacatagctt gaattgtact gtcattttta
     1861 tgtttgtaaa caaaaggcac aaacgaagct aaaaatctat tgggatatgg ttttttatat
     1921 atctgttcta aaacttgagc taatgtaaat tgaaaggtta cattccattc atcggatatt
     1981 gattgcgata atttattata atataataat tggataaaac ttttaccaat atttgcacca
     2041 ctcccctcat cgcccaatat ataaccgagt gaaggcatca tatcttgtat ttgatttcca
     2101 ttccaatatg ctatactcat accggttcca agaattgcaa caacacccgg tttatttcca
     2161 tacaatgcta tagctgcacc aactaaatca gaatcaatta caatttgcgc atttgtaaaa
     2221 aattgttcta acgctcttcg aactattatt tgtgattctt tggatttaca acctgcacca
     2281 taaaaatata cttgtgtaac tatacttgca ggcaatgtat atgaatatcc gttttgcaat
     2341 tcttctacaa tattttgtgt tgttacaaaa tacggattca acccttttgt atgaaacatg
     2401 aactttgctt cttcatgcac tatacaccaa tctgttttgg tagaaccact atctgcaatt
     2461 acaatcatgt atttttatat ttgagtaagc atagttttat atatgccaat agaattctta
     2521 aatttcaaca gtaatattgc atcaaaatca tcttgatatt ttccacaata taattcatat
     2581 tgttctttat tttcgaaaaa aacttgtaaa gcaaaacaat catctgcttc ctgctgtgtt
     2641 tctattttaa acaataaaac attttgaatt aaatcacatg catgcataag aggtatataa
     2701 gtatctttca tccaaagtat agcatcactt tgaatagatt ctgaaaatga taatgtttta
     2761 ttaataagta tcactatatg tgcgaattaa attgaatacg taaagaatta tacaataata
     2821 ctatgattat agatgaaact gaaaaaagta tttgcattag ggtttgaagc attgtattta
     2881 ttaaaatata gttttgcata tcaacagaaa gaaaactttc tat
//
LOCUS       DAOTOW010000236         2922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26584_length_2922_cov_2.669690, whole genome shotgun sequence.
ACCESSION   DAOTOW010000236 DAOTOW010000000
VERSION     DAOTOW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26584_length_2922_cov_2.669690"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="P5243_05185"
     CDS             complement(<1..1124)
                     /locus_tag="P5243_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRS18875.1"
                     /translation="MKKRFFVISVLLSVILSSCSHKLPYTSITNGVQVQIDSMYVKAQ
                     FYTNSIVRVQKWAAGSSDSKISLMVIIDSVQSLNISVSQSNGYIVLQSPELLVKISEK
                     DGAISYFDTQNQQIVAEKNAPDFTPYVSPNEKAWSVAQTFQLSNDEAIYGLGQHQYGY
                     MNYRGKEVKLVQTNTDAVIPFLWSTAGYGILWDNYSKTIFTDTDAGATLWSDVADNID
                     YYLIADKKADKVISGYRYLTGKAPMFGKWAYGYWQSKEHYNTQAELMAVAEKYRKLQI
                     PIDNMIQDWDYWNGAANWSGMFFDKTLFPRPQEMMDKLHAMNYHAIISIWPALGPNTP
                     IYKEMNEKGYLYKPVGWAGFKYYDAFNPEATKLYYQYLKSG"
     gene            complement(1140..2657)
                     /locus_tag="P5243_05190"
     CDS             complement(1140..2657)
                     /locus_tag="P5243_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRS18876.1"
                     /translation="MVYTGKIIILFFCCVISIQLSAQYQTDNSQYNSLYYKNPIFAGD
                     YPDPSILVDGNSYYVVHSSFEYYPGLTIWKSNDLLQWTPIQSPLKTYVGSVWAPDIVK
                     YQNKYYIYFPANNTNYVIYADSINGTWSKPIELRISSIDPGHFVDSKGNRYLYFSNGS
                     YVPLSKDGLQITGELTPVYKGWQIPREWSIECFCMEGPKLFKHGNYYYLTVAQGGTAG
                     PATGHMVISARSKTPFGPWENSPYNPIARATNTNETWCSVGHATVFKATTNDWWMILH
                     AYKNGNYSMGRQTLLAPLIWTNDGWFKLNDTLLLDAPIKKPQIHQAPFNSTIFTTFNS
                     LSFEHLWKFFGEYDPSRFSIKGNSIEIKSKGTNIAESSPLLYVPAHNSYSAEVEIEIR
                     GNAQAGLIMFYNQQCYSGILADSTNILTNLRGWQFVTEPNTINRHVFLRLINRNNTVD
                     MYYSIDGASWIKTTNSTEVSGYHHNVLSGFLSLRIGLVSMGTGSVQFKNFKYQAL"
     gene            complement(2659..>2922)
                     /locus_tag="P5243_05195"
     CDS             complement(2659..>2922)
                     /locus_tag="P5243_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS18877.1"
                     /translation="WGPICWVLIAEIFPNKIRAAAVAVAVAAQWAANYFISSTYPFMM
                     EYSGAVTYGFYGIMSILSFVFVWKMVPETKGKTLEEMEKIWEK"
BASE COUNT          892 a          556 c          521 g          953 t
ORIGIN      
        1 ccgcttttga ggtattgata atacaatttg gtagcttcag ggttgaatgc atcataatat
       61 ttgaatcctg cccaaccaac aggtttgtac aaatatccct tttcattcat ttctttataa
      121 ataggtgtat tcggtcctaa tgccggccat atagaaatta tagcatgata attcatagca
      181 tggagtttat ccatcatttc ttgtgggcga gggaaaaggg ttttgtcgaa aaacatacca
      241 ctccagttgg cagctccgtt ccagtagtcc caatcttgaa tcatattatc tattggaatt
      301 tgaagtttac gatatttttc tgctactgcc ataagttctg cttgtgtatt ataatgttct
      361 ttgctttgcc aatatccata agcccatttg ccaaacatag gagctttgcc tgtaagatag
      421 cgatatcccg aaatcacctt gtcggctttt ttgtcggcta tcaaatagta gtcaatattg
      481 tcggcaacat ctgaccatag tgtagcaccg gcatcagtat cggtaaatat ggttttggaa
      541 taattgtccc ataaaattcc atatcctgcg gttgaccaca aaaaaggaat taccgcatcg
      601 gtattggttt gcaccaattt tacctcttta ccgcgatagt tcatgtatcc gtattggtgt
      661 tgccctaagc catatatagc ttcatcgttt gacagttgaa atgtttgtgc tacactccat
      721 gctttttcgt ttggtgaaac gtagggagta aaatcaggag catttttttc ggctacaatt
      781 tgttgatttt gggtatcaaa atatgaaatg gcaccatctt tttccgaaat ttttacaagc
      841 aattccggag attgtaaaac aatatatccg tttgattgcg aaacagaaat gttgagagac
      901 tgcacactgt caataattac catcaaactg attttggagt cgctagaacc tgccgcccac
      961 ttttgtacac gtacaataga attggtgtaa aactgagcct ttacatacat gctgtctatt
     1021 tgtacttgta ccccattggt aatagatgtg tacggtaatt tgtgtgaaca actgctaaga
     1081 atcacactca aaagaacaga aattacaaaa aaacgtttct tcatatattt agtatttagt
     1141 tataatgctt gatatttaaa atttttgaat tgcacactgc ctgtacccat tgaaaccaac
     1201 ccaatacgta agctgagaaa tccactgagc acattgtgat gataccccga aacttcggta
     1261 gaattagttg tttttatcca cgaagcgcca tcaatactat aatacatatc tactgtattg
     1321 tttctattga taaggcgtaa aaacacatga cgatttattg tattgggttc ggttacaaat
     1381 tgccaaccgc gtaaattagt gagaatgttt gttgaatcag ctaatatacc cgagtagcac
     1441 tgttggttat aaaacataat taatcctgct tgagcgttac cccgaatctc aatttcaact
     1501 tcagcactgt atgaattatg agcaggaaca tacaataatg gtgaactttc tgctatattg
     1561 gtacctttgc tcttaatttc tatgctattt ccctttatcg aaaaacgaga ggggtcatat
     1621 tcaccaaaaa atttccataa atgttcgaat gatagtgaat taaaagttgt aaaaatagtt
     1681 gaattgaacg gtgcctgatg tatctgtggt ttttttattg gagcatcaag taagagtgta
     1741 tcattcagtt taaaccagcc atcgttagtc catattaaag gagccaataa tgtttgtcgc
     1801 cccatagagt agttaccgtt tttgtaagca tgcaatatca tccaccaatc gtttgtagta
     1861 gctttaaata cagtagcatg ccctacagaa caccaagttt catttgtatt ggttgctcgt
     1921 gctataggat tgtaaggtga attttcccat ggtccaaatg gagtttttga tcgtgccgat
     1981 attaccatat gtcccgttgc aggcccagct gtgccccctt gtgcaacagt taaataataa
     2041 taatttccgt gtttgaataa ttttggccct tccatgcaaa aacattcaat tgaccattct
     2101 cgaggaattt gccaaccctt atatacggga gttaattctc ctgtaatctg taacccgtct
     2161 ttcgacaaag gaacataact accgttgcta aaatacagat atctatttcc tttagaatca
     2221 acaaaatgac cggggtcgat acttgaaatt cgtaattcta taggcttact ccatgtacca
     2281 tttatggaat cggcgtatat aacataattt gtattatttg cagggaagta tatataatat
     2341 ttgttttgat actttactat atcgggagcc caaaccgaac ctacatatgt tttgagagga
     2401 ctttgtatgg gagtccactg aagtaaatcg ttcgatttcc aaatagtcaa tcccgggtaa
     2461 tattcaaacg aggaatgaac tacataatat gaattgccat caactaaaat actggggtcg
     2521 ggataatcgc ctgcaaaaat aggattttta taatataatg aattgtattg cgaattatcg
     2581 gtttgatact gagcagataa ttgtatactg atgacacaac aaaaaaaaag tattattatt
     2641 tttcctgtat ataccatgtt atttctccca aattttctcc atttcttcca atgtttttcc
     2701 tttggtttcg ggcaccattt tccatacaaa cacaaacgaa agaatactca tgattccata
     2761 aaatccatag gtaactgcac cactgtattc catcataaac ggataggtag acgatatgaa
     2821 ataattggca gcccattgag cagcaacagc aacagcaaca gcagcagcac gtattttatt
     2881 cgggaaaatt tcggcaatca gtacccaaca tattggtccc ca
//
LOCUS       DAOTOW010000237         2915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26665_length_2915_cov_2.853497, whole genome shotgun sequence.
ACCESSION   DAOTOW010000237 DAOTOW010000000
VERSION     DAOTOW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26665_length_2915_cov_2.853497"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /locus_tag="P5243_05200"
     CDS             complement(<1..1832)
                     /locus_tag="P5243_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS18878.1"
                     /translation="MNLVRASLKYKQVTLTVLLIVFIIGVYSLLTMPRREDPKITSPI
                     GLLVSYFPGANAIQVEDQVTHPIEELLFQNAEVCKEKTKSISRDGISLVYVWINEDVT
                     NPDVFWNKLRNQLLLIKQTKLPLEVVGPFVNSDFGDTEALLISIESDQVSNAQLKEYA
                     LMLENKIRSLPESSKIKRIGEQKQELTIQFNFQKLSQYDISLEQILQILKTQNVIFAS
                     GSITTDKYEIPIHTNGFYTNEEQIKNQIVATSSSGAVVRLRDIATITREYAKPESIIR
                     VNGNNAIIVGVQMHEGNNIVEFGKKVDDILTQLKKEVPSFVEITIISNQPELVNHNVT
                     HFLQEFLIAIIAVIIVVLLLLPFRIATVAATAIPMTIALTFAILHIFHIELHQVSLAS
                     LIVVLGMVVDDAIVVADNYVELLDKGISRWTAAWQSAYELIIPILTATITIIAAFLPI
                     ILLTGPIGEFIHDLPITVTVALSSSFVVAMFLTPILCFVFITKGLKEDLENDTKHHKK
                     SILIHMQNIYNKALEWCVEYPKTIIIVTIMSIAIAVLLFVYGVGHKFFPEAERNQFFI
                     ELWMPMGTQIEYTNACIKRVEKVIKNDKRITSYATFVGMSAPRV"
     gene            complement(1838..>2915)
                     /locus_tag="P5243_05205"
     CDS             complement(1838..>2915)
                     /locus_tag="P5243_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS18879.1"
                     /translation="VNYIHIYNYNIVKLQHMNTLAKFLKFFILLLFISCSHHKKTHDE
                     QKPLVVQTTYPTLHKKMKTITISGNIEGNITVRLGFLVSGKINSIRVQEGDIIQAGEI
                     LASLDPTSYEQAKEIADANLNQMQDDYARISQLYEKKSIAESEYIKISNGLKAAKAQQ
                     VLQTKNVNDTKLYSPITGIVLKKGVEVGEIIGQGIQVFAISDIKQVKVQCFVPASDLR
                     NISIGNKASVYVASLDSIFSGTIVEIGTLADAATRTFPIKIIIDNPNMLLRPGMTAEI
                     SIQTNMPEQSCMIPTECILHDVNNMPYVFIADTVSQKAFKILVSIGTISNNMIEITEG
                     LNVNHQVIVAGQHKLENGSSIIVK"
BASE COUNT          912 a          459 c          441 g         1103 t
ORIGIN      
        1 tacactcgtg gtgcactcat acctacaaat gtagcatacg atgttattcg tttatcattt
       61 ttaattactt tttcaacacg cttaatgcag gcgttagtgt attcaatttg tgtacccata
      121 ggcatccata attcaataaa aaattgattt cgttctgctt caggaaaaaa tttatgacct
      181 acaccatata caaataacaa tactgctatt gcaatactca taattgtaac tattataata
      241 gttttgggat attcaacaca ccattctaat gctttgttgt atatgttttg catatgaatt
      301 aaaatagatt ttttatggtg ttttgtgtcg ttttcgaggt cttcttttaa gccctttgta
      361 ataaatacaa aacataaaat aggagttaga aacattgcaa ctacaaatga agaagacaaa
      421 gcaacagtta ctgtaatggg taaatcgtga ataaattcac caataggtcc tgttaataat
      481 atgattggca aaaatgcagc tataatagta atagttgcag ttaaaatagg aattatgagt
      541 tcatatgcac tttgccatgc tgccgtccaa cgcgatattc ctttatctaa aagttctaca
      601 tagttatcgg ctaccactat ggcatcatca actaccattc ccagtaccac aattaacgac
      661 gccaatgata cttgatgtaa ttcaatgtga aatatatgaa gtatagcaaa tgttaaagct
      721 atagtcatag gaattgctgt agctgctacc gtggcaattc tgaatggaag tagtaataaa
      781 acaacaatga taacagctat aattgcaatt aaaaattctt gtaaaaaatg ggttacattg
      841 tggttaacta attctggctg attagaaata atagtaattt caacgaatga gggaacttct
      901 ttttttaatt gagtaagaat gtcatcaact tttttcccaa attctacaat attgttaccc
      961 tcatgcattt gcacacctac aataatagca ttatttccat ttactcttat tattgattca
     1021 ggtttagcat attcacgtgt tatggtagct atatctctta atctaacaac tgcaccagag
     1081 cttgaagttg ctacaatctg attcttaatt tgttcttcat tggtataaaa cccattggta
     1141 tgtataggaa tttcatattt gtcggtggta atacttccac ttgcaaaaat tacattttgt
     1201 gtttttaaga tttgtaaaat ttgttccaga gatatatcgt attgtgataa tttctgaaaa
     1261 ttaaattgaa tggttagttc ttgtttttgt tcacctattc ttttaatctt agaactttcc
     1321 ggcaaacttc gtattttatt ttcgagcatc aatgcatatt ccttgagttg tgcatttgat
     1381 acttggtctg attctatact tatgagtaaa gcttcggtat ctccaaaatc agaatttaca
     1441 aaaggaccaa ctacttcaag tggtagtttt gtttgtttaa tcaataaaag ttgatttcgt
     1501 aatttattcc aaaaaacatc cggatttgta acgtcttcat ttatccatac atatactaaa
     1561 gaaattccat cacgtgaaat agatttcgtt ttttctttgc aaacttctgc attttgaaat
     1621 aatagttctt caatagggtg agttacttga tcttctactt gtatagcatt tgcaccggga
     1681 aaatatgaaa cgagcaatcc tatgggtgaa gtaattttag gatcttcgcg tcgtggcata
     1741 gtgagcaatg aatatacgcc aattataaac actataagta atactgtaag tgttacttgt
     1801 ttatatttta atgatgcacg tacaagattc atatatttta ttttacgatt attgaactac
     1861 cattttctaa cttatgttga cctgctacaa ttacttgatg attgacattg agtccttcgg
     1921 taatttctat catattatta gaaattgtac caattgaaac cagtatttta aatgcttttt
     1981 gagatacagt gtctgcaata aaaacataag gcatattgtt tacatcatgt aaaatacatt
     2041 cagttggaat catgcaactt tgttccggca tattggtttg tattgaaatt tctgctgtca
     2101 tacccggacg taacaacata tttggattgt caatgattat ttttatagga aacgtgcgtg
     2161 tggcagcatc ggctaatgtc ccaatttcta caatagttcc cgaaaatata gaatctaatg
     2221 aagcaacata taccgatgct ttattgccaa ttgatatatt tcttaaatcc gatgcaggca
     2281 caaaacactg tacttttact tgttttatat ctgaaattgc aaaaacttga attccttgtc
     2341 caattatttc tcctacttca acaccttttt ttaatacaat tccggtaata ggagaatata
     2401 gtttggtatc atttacattt tttgtttgta atacttgttg cgcctttgct gcttttaatc
     2461 catttgaaat tttaatatat tcactttcgg caatactctt tttttcatat aattgagaaa
     2521 ttcgtgcata gtcatcttgc atttgattca aatttgcatc ggcaatttct tttgcttgtt
     2581 cataacttgt agggtctaat gaagcaagta tttctcctgc ttgtattata tctccttctt
     2641 gtactcttat tgaatttatt tttcctgata ccaaaaaacc taaccttacg gttatattac
     2701 cttctatatt accgcttatg gtaattgttt tcattttttt atgtaaagtt ggatatgtgg
     2761 tttgaaccac taagggtttt tgttcgtcat gtgttttttt gtgatgtgaa caacttataa
     2821 ataataataa aataaagaat ttcagaaatt ttgcaagagt gttcatatgt tgtagtttta
     2881 caatgttata gttatatata tgtatataat ttaca
//
LOCUS       DAOTOW010000238         2913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26685_length_2913_cov_3.154654, whole genome shotgun sequence.
ACCESSION   DAOTOW010000238 DAOTOW010000000
VERSION     DAOTOW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26685_length_2913_cov_3.154654"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="P5243_05210"
     CDS             <1..367
                     /locus_tag="P5243_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18880.1"
                     /translation="IPHVLVDVFKPQQTDLIFEQAKPLAIRMLQLASLYVLAEAILVS
                     FVGALRGAGDTVWTMWVSMLIHWTFVPTAYLSLFVFNLGPIIAWTILVFMFLLFCIVF
                     YFRFKTGKWKTIQVIQHGE"
     gene            complement(386..1876)
                     /locus_tag="P5243_05215"
     CDS             complement(386..1876)
                     /locus_tag="P5243_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18881.1"
                     /translation="MKLFHFLFCSFFIFNIYGQTTIVQRIEFASENQITQTIPFGNNG
                     LLYQNNIEKKAKQDKNNTVNSYTLFNTNLDKVKTIDVKTPKKYSVHLESKSAHKFFEI
                     AIDKHKNYIIHAISIPQLDVTSLHGKLPKNLYIHAVESLDDYIFIKASTRKFYFIFIK
                     NMITGEESFTQYTPVSAHDFNMLGFKADSSSNELYIIYRDKINNEITTIVEIYTQGKK
                     SNEFIIPNGTEKFLLNGSLFKTKDNAYILCGTFGNSHKDMMSVGIYICKLKHKKIEFI
                     QFVNYLDIKNFMINLPEKKQERIIKRKEKKEKKGNDIFISYMIAPHKIIEYNNEYILI
                     AEAFYPTYKQECYTVPTGSGGTTTQCTVVFNGYEYTHYFIYTFDQKGNPIWSNTKKMN
                     ISYKPFRVETFVNANITNNNLVTLYGTDKEFYYTAFNQKGEEISSETHISITSENESE
                     SIKKSSMITEHWYDNFFIAYGFQKIKNTDEKEKRNVFFINKIEVKP"
     gene            complement(1900..2250)
                     /locus_tag="P5243_05220"
     CDS             complement(1900..2250)
                     /locus_tag="P5243_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS18882.1"
                     /translation="MKKKILVVDDSSSNILLLQNFLEDEGFDIQVASSGKEAIKKVDD
                     FNPDLILLDLMMPGVNGLDVMHSISKDIPVIMISANKDPKLVNEAKAKGIKAYIQKPI
                     DFDEILNEIAIHIK"
     gene            complement(2313..>2913)
                     /locus_tag="P5243_05225"
     CDS             complement(2313..>2913)
                     /locus_tag="P5243_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRS18883.1"
                     /translation="ENILQIASCDIDILGFILYEKSARFIGYDFDSQIIASCSKKTAG
                     VFVNHPIEYVIQKQQTYLFDFLQIHGNESPEYCKELQQQTNAYIIKAFLIDENFSDTL
                     LQKYSPYCDYFLFDTKTEVYGGSGKKFNWNMLHSMNINKPFFLSGGITPNDIESIVDF
                     KHSLLFGIDINSKFELQPALKNVNSVHTFAQQIKTHQKK"
BASE COUNT          916 a          375 c          429 g         1193 t
ORIGIN      
        1 tattccacac gtcttggttg atgtgtttaa acctcaacaa actgatttga tttttgaaca
       61 agcaaaacca ttggcaatac gtatgttaca attggcttca ttatatgtat tggcagaagc
      121 tattttggtt tcgtttgttg gagcattgcg tggagccggt gatacagtat ggactatgtg
      181 ggtttctatg ttaattcatt ggacgtttgt gccgacagcg tatctttctt tgtttgtatt
      241 taatttaggc ccaatcatag catggactat tttggtgttt atgtttttat tattttgtat
      301 tgtattttat tttcgattca aaacaggaaa atggaaaact atacaagtaa ttcaacatgg
      361 agaataatta gtgatttgcc taattttaag gctttacctc aattttattt ataaagaaca
      421 catttcgttt ttctttttca tcagtatttt taattttttg aaaaccgtaa gctataaaaa
      481 aattatcata ccaatgttcg gtaatcattg aagatttttt aatagattct gattcgtttt
      541 cagaggtaat agatatatga gtttctgatg aaatttcttc tcctttttga ttaaaagccg
      601 tataataaaa ttctttgtcg gtaccataca atgtaactaa attgttgttt gtaatatttg
      661 catttacaaa cgtttcaaca cggaatggtt tgtatgaaat attcattttt ttagtattac
      721 tccatatagg atttcctttt tgatcaaatg tataaataaa ataatgggta tattcgtaac
      781 cgttaaatac aacagtacat tgagttgttg taccgccgga acctgttgga acagtataac
      841 attcttgttt atatgttgga taaaaagctt ctgcaattaa tatatattcg ttattatatt
      901 ctattatttt atgtggcgct atcatgtagc ttataaatat atcatttcct tttttctctt
      961 ttttttcttt tcgttttatg attctttctt gttttttctc cggcaaatta atcataaaat
     1021 ttttaatgtc taaataattt acaaattgta taaattcaat ttttttatgt ttcagtttac
     1081 atatatatat tccaacagac atcatgtctt tatggctatt gccgaatgtg ccgcatagta
     1141 tatatgcatt atctttggtt ttaaataatg aaccattgag tagaaatttt tcagtaccgt
     1201 taggtataat aaattcgttt gattttttac cttgtgtata tatttcaact atggtagtaa
     1261 tttcattgtt tattttatcg cggtatatta tatacaattc attgcttgat gaatctgctt
     1321 taaaacctaa catgttaaaa tcatgcgcag atactggtgt atattgtgtg aatgattctt
     1381 caccggttat catgtttttt atgaaaataa aataaaattt acgtgtgctt gcttttataa
     1441 atatgtaatc gtcaagagat tctacagcat gtatatataa atttttaggt aatttcccat
     1501 gtaaagatgt tacatctaac tgtggaattg aaatagcatg aattatataa tttttgtgtt
     1561 tgtcaatagc tatttcaaaa aatttgtgtg cactttttga ttctaaatgt acactatatt
     1621 tcttgggggt ttttacgtct atcgttttta ctttatctaa attggtgttg aaaagagtat
     1681 aggaattaac agtattattt ttatcttgtt ttgctttttt ttctatgttg ttttgatata
     1741 ataatccatt attcccaaaa ggaatagttt gtgtaatttg attttctgaa gcaaattcaa
     1801 ttctttgtac aatagttgtt tgaccatata tgttgaaaat gaaaaaacta caaaataaga
     1861 aatgaaataa tttcattttt ataagaattg tgcaatacgt tattttatgt gaatagcaat
     1921 ttcattcaaa atttcatcaa aatctatagg cttttgaata tatgctttaa taccttttgc
     1981 ttttgcctcg ttaactaatt tagggtcttt attggcagaa atcataataa ccggaatatc
     2041 tttagaaata gaatgcatta catctaatcc gtttacaccg ggcatcataa gatccaataa
     2101 aattaaatca ggattgaaat cgtcaacttt tttaatagct tctttaccgg aactagcaac
     2161 ttgaatatca aatccttcgt cttctaagaa gttctgcagt aaaagtatat tcgatgatga
     2221 atcatctact actaatattt ttttcttcac gttttttagt ttttaagatt cattcaaaaa
     2281 tataaaaaaa aggagttatg cacaattttt ttttattttt tttgatgcgt ttttatttgt
     2341 tgagcaaatg tgtgaacgct atttacattt tttaatgcag gttgtaattc aaattttgaa
     2401 ttaatatcga taccaaataa taatgaatgt ttaaaatcta ctatagattc aatatcgtta
     2461 ggagtaatac ctccgcttaa aaaaaatggt ttgtttatat tcatagaatg taacatgttc
     2521 caattgaatt tctttccgct accaccatat acttcagttt ttgtgtcaaa taaaaagtaa
     2581 tcacagtaag ggctgtattt ttgtaaaaga gtatcagaaa agttttcgtc aattaaaaag
     2641 gcttttatta tatatgcgtt tgtttgctgc tgtaattctt tacaatattc tggtgattcg
     2701 ttaccgtgta tttgtaaaaa atcaaataaa tatgtttgtt gcttttgaat aacatattca
     2761 atagggtgat ttacaaatac gccggcagtt tttttggaac atgatgctat gatttgagaa
     2821 tcaaaatcat agccaataaa acgagctgat ttttcgtata aaataaatcc taaaatatct
     2881 atatcgcaag aagcaatttg gagtatattt tct
//
LOCUS       DAOTOW010000239         2906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26767_length_2906_cov_2.567520, whole genome shotgun sequence.
ACCESSION   DAOTOW010000239 DAOTOW010000000
VERSION     DAOTOW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26767_length_2906_cov_2.567520"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="P5243_05230"
     CDS             complement(<1..1235)
                     /locus_tag="P5243_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRS18884.1"
                     /translation="MEKKYSLLNAIFGWVAFAGAAIVYLLTMEPTVSLWDCGEFIATA
                     AKLEVGHPPGAPLFLMLGRFFALFASDPSTIAMCINSLSALASAATIMFLFWTITHLA
                     LKFYNKQSLTASQIYVVLAAGLIGAGAYTFTDTFWFSAVEGEVYALSSLFTAVVFWAI
                     LKWENVADTPRSDKWLILIAYIMGLSIGVHLLNLLTITAIVYVYYFKKFTPTTKGIIL
                     AGALSLLIIAFMMWGIITGSMEVASQFELFFVNVLGLPFDSGLIIYLFVLAAALIAGI
                     ILTKRDVANKIKVGIVTLSAILIGMPFMSDSILIWLLLIAGIIWLLNYITKQNPTVLN
                     TIVTIMAVIMIGYSSYAMIIIRSNANTPMNENAPDNVFSLLSYLNREQYGSTPLLYGP
                     YYNAAPEWKADNSAEVNEK"
     gene            complement(1346..2377)
                     /gene="thiL"
                     /locus_tag="P5243_05235"
     CDS             complement(1346..2377)
                     /gene="thiL"
                     /locus_tag="P5243_05235"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRS18885.1"
                     /translation="MTKQTTLAEIGEFGLIERITKQSTKIHKTTGYGIGDDCAVLKYT
                     DTLYTLISTDLLVEGIHFDLVYTPIKHLGYKAVICNISDIYAMNGTPQQITVSIAVSA
                     KFSVEAIEQLYEGIHTACKAYNIDLIGGDTTSSITGLTISITAIGIVAESDISYRKTA
                     RVNDLICVTGNLGAAYMGLQILQREKSIFKEVGAQPKLEGYEYVLQRQLKPESPKHII
                     EQLKKNGIIPTAMIDISDGLSSELFHICKQSHVGCNIYEEKIPIHENTADVCKEFSIE
                     PLIPALHGGDDYELLFTIPITCFDSISTIQGVTVIGNITEADKGLYLISKAGDKVALK
                     AQGWTSFTQ"
     gene            2718..>2906
                     /locus_tag="P5243_05240"
     CDS             2718..>2906
                     /locus_tag="P5243_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS18886.1"
                     /translation="MLVSIFDISLPLNNPVLQFSIILFIILFAPIILNKFKIPHLIGL
                     IIAGALIGPFGFNLMLRDS"
BASE COUNT          977 a          450 c          459 g         1020 t
ORIGIN      
        1 aatttttcat tcacttcggc agaattgtct gctttccatt caggagcagc attataatat
       61 ggaccgtaca ataatggagt actaccgtat tgttcgcggt taaggtacga aagtaatgaa
      121 aatacattgt caggagcatt ttcgttcatt ggagtattgg catttgaacg aataataatc
      181 atggcatacg aagaatatcc tatcataatt actgccataa ttgttactat agtattgagt
      241 actgtagggt tttgtttagt gatatagttt aataaccata taataccagc tataagcaac
      301 aaccaaataa gtatactgtc ggacataaaa ggcattccta ttaatatagc actaagagtt
      361 actataccta ctttaatttt atttgcaaca tcacgttttg taagtataat accggcaata
      421 agagcagcag caagtacaaa tagataaatt attaatccgg aatcgaaagg taatcctaat
      481 acatttacaa aaaataattc aaattgagac gctacttcca tagaacctgt aataataccc
      541 cacatcataa aggctataat aagtaacgat aatgctcctg caagtataat tcctttggtg
      601 gttggtgtaa attttttgaa ataatatacg tacacaatag ctgtaatggt aagtaagtta
      661 agtaagtgaa cacctataga taatcccata atatatgcta taagtatcaa ccatttgtca
      721 ctacgggggg tatctgcaac attttcccat ttcaaaattg cccaaaatac taccgcagta
      781 aagagtgacg ataatgcata aacttcgcct tcaactgccg aaaaccaaaa tgtgtcggta
      841 aaagtatatg ctccggcacc tataagaccg gctgccaata caacatatat ttgagatgca
      901 gttaatgatt gtttgttata gaatttaaga gccaaatgag taattgtcca aaacaaaaac
      961 ataatagttg ccgcacttgc taatgcagat aatgaattaa tacacattgc aatagtagat
     1021 ggatcacttg caaacaacgc aaagaaacgg cccaacatta aaaataatgg tgcaccggga
     1081 gggtgaccta cttctagttt tgcagctgtt gctataaatt caccacagtc ccataaactt
     1141 actgtaggtt ccatagttaa caagtaaact attgcagctc ctgcaaatgc tacccatccg
     1201 aaaattgcat tgagtaatga atattttttt tccatattgt aattttttat attcaaatgt
     1261 tgaacggcga aattaccgtt tttttttgaa tttttattgt taatacgtat taaaatgccg
     1321 tcgaataaac agtaaaatta aataattatt gtgtaaacga agtccaacct tgagctttaa
     1381 gtgcaacttt atctccggct ttcgaaatta aatataaacc tttatcagct tcggttatgt
     1441 ttccaattac agtaactcct tgaatagtag aaatagaatc aaaacatgta ataggtattg
     1501 taaagagtaa ttcatagtcg tctccaccat gtaatgcagg gataagaggt tctatagaga
     1561 attctttaca aacatctgca gtattttcat gaattggaat tttttcttca taaatattgc
     1621 atcctacatg agattgtttg catatatgaa ataattctga tgaaagtccg tcagatatgt
     1681 caatcatagc tgtaggaata ataccgtttt ttttgagttg ttcaataata tgttttggtg
     1741 attccggttt taattgtcgt tgtaatacat attcgtatcc ttcaagtttt ggttgagctc
     1801 caacttcttt gaatattgat ttttcacgct gaagtatttg tagccccata tatgctgcac
     1861 ctaaattacc ggttacacaa attaagtcat ttacgcgtgc tgtttttcta tagcttatat
     1921 cagactctgc aactattcct atagcagtaa tagaaattgt taatccggta atagaagatg
     1981 ttgtgtcgcc acctattaaa tctatattat atgctttaca agcagtatga attccttcat
     2041 ataattgttc tattgcttct acgctaaact ttgcagatac agcaatgctt accgtaattt
     2101 gttggggggt tccgttcatt gcataaatgt ctgaaatatt gcatattact gctttgtatc
     2161 ctaaatgttt tataggagta tacactaaat caaaatgaat accttctacc aataaatctg
     2221 ttgaaataag agtatatagt gtgtcggtat attttagaac agcacaatcg tcgcctattc
     2281 catatcctgt tgttttatgt attttggtac tttgttttgt aattcgttct attaacccaa
     2341 attctcctat ttcggcaaga gttgtttgtt ttgtcatttt gtaatttttt gtcaaaaata
     2401 caaaattgtt tgcaataaga gatacattaa agatataaag caggaaaata atggttagaa
     2461 ttgttttgta ttttttgaaa tgaaatgcga atgattttta tgtaatgttg aaataataat
     2521 ttagcaaata tgttgttgag ttaaaattac ataatattat aggtgtttat gagtaatgtt
     2581 ttttagagag tctggttatg tttattttat atttattgaa aaaaacaaaa aaacgttata
     2641 taaattatat ttgaaatctt ttttgtaaaa gtttacaata aaaaaaatgt atttttggca
     2701 aaaaagaaac tacacatatg cttgtatcaa tttttgatat atcactacct ctcaataatc
     2761 ctgtattaca gttctctatt atacttttta taatattatt tgcccccata atacttaata
     2821 agtttaaaat tcctcattta ataggtctca ttatagccgg agcacttata gggccttttg
     2881 gatttaattt aatgttacgt gacagt
//
LOCUS       DAOTOW010000240         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26865_length_2897_cov_3.347291, whole genome shotgun sequence.
ACCESSION   DAOTOW010000240 DAOTOW010000000
VERSION     DAOTOW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26865_length_2897_cov_3.347291"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5243_05245"
     CDS             complement(<1..271)
                     /locus_tag="P5243_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRS18887.1"
                     /translation="MNLDSLTAISPIDGRYHDKTEKLSLYFSEHALIYYRVRVEIEYF
                     IALCNASIPQLHGINSDMFKQLRTIYTSFSIADAQRIKDIEKTTNH"
     gene            354..680
                     /locus_tag="P5243_05250"
     CDS             354..680
                     /locus_tag="P5243_05250"
                     /inference="COORDINATES: protein motif:HMM:NF021468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HRS18888.1"
                     /translation="MAKTYIIPEQNIQELIKPIGYCIASNRITVDGAQVGFMYREAPE
                     DEDDSGWRFVAGDETQEYLDDTLNFMMFDVNYIANIDRAIIPYLHLKKGTELERVQGT
                     DTFVPY"
     gene            851..1225
                     /locus_tag="P5243_05255"
     CDS             851..1225
                     /locus_tag="P5243_05255"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRS18889.1"
                     /translation="MESLLIQNTEDTPQIDFDLGTGIFSISGRSLPENAIEFYMPILE
                     WIEKVLSETSGKTYIFEIKLEYFNTASSKQLAKMLLMIEKYNELHNIIIRWCYEKEDN
                     DMLVSGNQYSKFLKLKFEFIEY"
     gene            complement(1241..>1773)
                     /locus_tag="P5243_05260"
     CDS             complement(1241..>1773)
                     /locus_tag="P5243_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRS18890.1"
                     /translation="RIDMLCSVPAIALSMALSTFVGQNMGARKIDRVVKGYTITLSIG
                     IGISLLLSLITIIFRHQIMSLFTNELTIIPIGVDYLMIVSPFYFAFAILFISNGVLRG
                     AGDTIIPMFVTLFALWGIRVPLSYWLSTLFGEIGIWWGIPAGWLFGMTFSTAYYLLGR
                     WKKKIIVRKRIQEIEE"
     assembly_gap    1774..1783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1784..2600)
                     /locus_tag="P5243_05265"
     CDS             complement(<1784..2600)
                     /locus_tag="P5243_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRS18891.1"
                     /translation="MKDLSVGKESKLIIRFAIPLLLGLVFQQLYSVADSIIVGKFLPN
                     GEQALAAIGAAFPVIFFLISLVVGISSGATIIISHYFGDKDYEGIQKAVDTINIFLVI
                     CAIIISVIGIVFAQSIFEYISIPADVIPDAVTYMQIYSSGFILMFGFHGVSAILRGVG
                     DSRKPLYILIISSILNVLLDIVFIIVFKTGIAGVAWATVVSQGLTFIAIVLYLNKTHP
                     ILRMRLRNIVFDYDIFKQSVKIGLPSGMQQAFVGLGSVALMAIVSKFGTVAVAA"
BASE COUNT         1105 a          432 c          408 g          942 t
ORIGIN      
        1 catgattggt agttttttca atatccttta tgcgttgagc atcggcaata ctaaatgatg
       61 tatatatagt tcgaagttgt ttaaacatgt cagaatttat accatgtaat tggggtattg
      121 aagcattgca caaagcaata aaatattcta tttctaccct tactctatag tatattaaag
      181 catgttcaga aaaatacagt gataattttt ctgttttatc atggtatctt ccgtctattg
      241 gagatattgc agtaagcgaa tctaaattca tatattaaga ttttaaaaat gtaaaaatac
      301 aataaattag ttacgcaaaa aaatagtatt tttgatatat aaaaaaatta catatggcaa
      361 aaacatatat tatacccgaa caaaacattc aagaattaat aaaacctatt ggttattgta
      421 tagcctcaaa cagaataact gtagatggtg ctcaagttgg gtttatgtat agagaagctc
      481 ctgaagatga agatgatagc ggttggcgtt ttgtagcagg tgatgaaact caagagtatt
      541 tagacgacac gttaaacttt atgatgtttg atgtgaatta tattgccaat attgatagag
      601 caattattcc atacttacat ttaaaaaaag gaactgaatt ggaacgagtt caaggaacag
      661 acacatttgt tccatattaa aaatatgtaa ttgtatactt atttgaaaaa tattcgttat
      721 aacacatgag ttaatagaga agaaattgta aaaaatgaaa aaaaaaatgc tttatcaatt
      781 ttttttacga taaattttgt tatttttaat tttacacata tttttacaaa aaaaactagt
      841 actatcgatt atggaatcat tacttattca aaatactgaa gataccccac aaattgattt
      901 tgatttgggc acaggaattt tttcgatttc aggtcgatca ctccccgaaa atgctattga
      961 attctatatg cctattttgg aatggataga aaaggtacta agtgaaactt caggaaaaac
     1021 atatatattc gaaataaaat tagaatattt taacacggca tcttctaagc aattggcaaa
     1081 aatgcttctt atgattgaaa aatacaatga acttcataat attattattc gttggtgtta
     1141 cgaaaaagaa gataatgata tgttagtttc cggaaatcaa tattctaaat ttttaaaatt
     1201 gaagtttgaa tttatagaat attaatattg tataaattat ttattcttct atttcttgta
     1261 ttctttttct tacaattatt ttctttttcc atctgcctaa tagataatag gctgtagaaa
     1321 aagtcatacc gaataaccac ccagccggta taccccacca aataccaatt tcgccaaata
     1381 gagtactaag ccaatacgat aaaggaacac gtatacccca taatgcaaat aatgttacaa
     1441 acataggtat tatagtatct ccggcgcctc gtaacactcc atttgaaata aataatatgg
     1501 caaatgcaaa ataaaatggg cttactatca ttaaataatc tacaccaata ggaattatag
     1561 ttaattcatt ggtaaacagt gacataattt gatgacggaa aataatagta attaaactta
     1621 atagtaaaga aatacctatt ccaatgctta aggtaatagt atatcctttt acaactctat
     1681 ctattttacg tgctcccata ttttgcccaa caaaggttga cagagccata gacagagcaa
     1741 ttgcaggaac cgaacataac atatctattc gtcnnnnnnn nnnatgctgc tacagctaca
     1801 gttccaaatt ttgaaactat tgccataaga gctacacttc ctaaacctac aaatgcttgt
     1861 tgcattcccg aaggtaatcc tatttttaca ctttgtttaa aaatatcata atcaaataca
     1921 atgtttcgta aacgcattcg taaaatggga tgagttttgt tgagatagag aacaattgca
     1981 ataaatgtaa gtccttgaga tactacagtt gcccatgcta cccctgctat tcctgtttta
     2041 aatactatta tgaaaactat atctaagagt acatttaaaa tagatgatat aataagaata
     2101 tagagcggtt ttcgagaatc acctacacca cgtaatatag cactgactcc atgaaatcca
     2161 aacatgagaa taaatcccga actgtaaatt tgcatataag taacggcatc gggaattaca
     2221 tcggcaggta ttgaaatata ttcaaaaata gattgtgcaa aaacaatccc tattacacta
     2281 ataattatag cacaaatcac taaaaatata ttaattgtat ctactgcctt ttgaatacct
     2341 tcatagtctt tatcaccaaa atagtgcgat atgataattg tagcaccact tgaaatacct
     2401 acaacaagcg atataagaaa aaagataaca ggaaatgcag ctccaatagc agcaagagct
     2461 tgttctccat taggcaaaaa tttacctact attatactat cggcaacact gtataattgt
     2521 tgaaaaacta aacctaataa tagtggaata gcaaaacgaa ttattaattt tgactctttg
     2581 cctacagata aatctttcat tagtcataaa atattaacaa atgtagttat ttattggaag
     2641 caacaaagcg tattgtcgac taaatttttt taaagaaata attgaataat tcactttgta
     2701 atcctgtttt ataaaattca gtaaatcaat attttacaat tatttgttgt aaaataaaaa
     2761 aataaaatca atactatatc ttttttattt ttatattcgt acaaatgagt acaaagtatt
     2821 gacaatgaaa acaataatta ccatagtagc aaccgtgttt ataagatttg gggcaatagc
     2881 cgaaggaact aaacaac
//
LOCUS       DAOTOW010000241         2896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26874_length_2896_cov_2.784231, whole genome shotgun sequence.
ACCESSION   DAOTOW010000241 DAOTOW010000000
VERSION     DAOTOW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26874_length_2896_cov_2.784231"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="P5243_05270"
     CDS             complement(<1..1800)
                     /locus_tag="P5243_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRS18892.1"
                     /translation="MNYPAGIEQKIGFDSIRTHIEYYCKSDIGKQIVANMQFVSNIDT
                     IQNSLNQIAEMKQIIEFEHDFPTPSFIHISSSLQSVIPFETWLQASEMHVLRLSLLEI
                     DAFIHFFSSHKDIYPALYSLCKEIAAPIELCKYIDQKIDVNGNIKDNASKKLIEIRQL
                     MQQKKQIVAKIMNQKLRNARAEGWCDSDTELTLRNGRLVIPLNTAFKRKIQGFVHDES
                     ITGKTSYVEPIEAFEINNDIVSLEYEEHKEIISILKELTNQIRPYINQIIDAYNLMAC
                     IDAIRAKALFSIRIQANKPQVIKKTYISLVGAKHPLLILSFEHTKREVIPLKVHLDHS
                     QRILVISGPNAGGKSVCLKTIVLLQYMCQCGLLIPASEHSTIGIFSSIFIDIGDEQSL
                     ENDLSTYSSHLRNMKYFVNGATHNTLFAIDEFGTGTEPLMGGAIAESVLEHLHNSGAY
                     GIITTHYTNLKHATTSLMYAVNGAMLFDLEQLKPLYKLRMGSAGSSFAFEIAAEIGLP
                     KSIIDQAKQKLGKDHVDFDRNLKQLEEEKQYIFKKKDELKKREQELKELQEKLHEKTT
                     NLNEKKLEIITHTKQEMKELLNSVNKQIEQTIRV"
     gene            complement(1816..2496)
                     /locus_tag="P5243_05275"
     CDS             complement(1816..2496)
                     /locus_tag="P5243_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS18893.1"
                     /translation="MKRFFILCLCVVSLGISAQNLKPTNDLALVTVTITNMKGSPSVG
                     DLVRFKSKKTGKIYSGTSTANGKFQILLPKETFAVLIQGFETEDTKNELVIPNVAGEI
                     TFNYTIKYELPKVFVLENVHFDVGKTSIRKDSYESLNNLAELMRRKKNMIIELAGHTD
                     NVGDAAANQKLSEQRAESVKNYLIQKGVPAQRIIAVGYGSTKPVASNETEQGRQKNRR
                     TEVNILHE"
     gene            complement(2654..>2896)
                     /locus_tag="P5243_05280"
     CDS             complement(2654..>2896)
                     /locus_tag="P5243_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRS18894.1"
                     /translation="CGAFGTIMVGAFAVEGGLFYGGGFKYIVTQLTGVAAVAAWSIGT
                     GLILFYGLKYLIGIRVSRKEEENGLDYYEHGEKAYN"
BASE COUNT          940 a          460 c          406 g         1090 t
ORIGIN      
        1 tacacgtata gtttgttcta tttgcttgtt tacagagttc aataactctt tcatttcttg
       61 tttggtatgt gttattattt caagcttttt ctcgtttaaa tttgtagttt tttcatgtaa
      121 tttttcttgt aattctttta attcttgttc gcgttttttt agttcatctt ttttcttaaa
      181 aatgtactgt ttctcttctt cgagttgttt taaatttcta tcaaaatcta cgtgatcttt
      241 tccaagtttt tgtttggctt gatctataat tgatttaggt aaaccaattt ctgcagctat
      301 ttcaaaagca aatgaactgc cggcagatcc cattcgtaat ttatataaag gttttaattg
      361 ttctaaatca aataacattg ctccatttac tgcatacata agagatgtag tagcatgttt
      421 aagatttgta tagtgagttg taattattcc atatgctcca gaattatgta aatgctccaa
      481 aacactttct gcaattgcac ctcccattag aggttctgta ccggtaccaa attcatcaat
      541 agcaaataat gtattatgag tagcaccatt tacaaaatat ttcatatttc gcaaatgaga
      601 actatacgta cttaaatcat tttctaatga ttgttcatca ccaatatcaa taaaaattga
      661 tgaaaatata ccaattgtac tatgttcgct tgcaggtata agtaaaccac actgacacat
      721 atattgcaac aaaacaatag tttttaaaca tactgattta ccacctgcat tgggaccgga
      781 aatgaccaaa attctctgcg aatggtctaa atgaactttt aaaggaatta cttcgcgttt
      841 tgtatgctca aatgaaagta tcaaaagagg atgtttagct cctacaagag aaatatatgt
      901 tttttttata acttgtggtt tatttgcctg aattctaata gaaaaaagag cttttgctct
      961 aatagcatca atacaagcca ttaaattata tgcatctata atttgattaa tatatggtcg
     1021 aatttgattt gttaattctt taagtataga aataatttcc ttatgttctt catattcaag
     1081 tgaaactata tcattattta tttcaaaagc ttctattggt tctacatatg acgttttgcc
     1141 tgtaatagat tcatcatgta caaacccctg tatttttcgt ttaaatgcag tatttaatgg
     1201 aataactaaa cgtccattac gtaaagtaag ttctgtatcc gaatcgcacc aaccttcagc
     1261 tctggcattt cgtaattttt gattcattat tttggcaaca atttgttttt tttgttgcat
     1321 taattgtcta atttcaataa gtttttttga agcattatct tttatattac catttacatc
     1381 aattttttga tcaatatatt tacataattc tatgggtgca gcaatttctt tacataatga
     1441 atataaagcc ggatatatat ctttgtgtga tgaaaaaaaa tgaataaagg catctatttc
     1501 aagtaatgat aatcgtaaaa catgcatttc tgaagcttgc agccatgttt caaatggaat
     1561 aacagattgc aatgaagatg aaatatgaat aaatgatgga gtagggaagt catgctcaaa
     1621 ttcaataatt tgcttcattt cagcaatttg attcaaagaa ttttgaattg tatcaatatt
     1681 gcttacaaac tgcatgttgg caacaatttg tttgcctata tcacttttac aatagtattc
     1741 tatatgagtt cgtatagaat cgaagccaat tttttgttct ataccagcag gataattcac
     1801 aaatgaatga tagttttatt catgtaatat atttacttcg gttcgtctgt ttttttgacg
     1861 tccttgttct gtttcattgc ttgctaccgg tttagtagaa ccatagccaa cagcaataat
     1921 acgttgtgca ggaacacctt tttgaattaa ataatttttt acactttcgg cacgttgttc
     1981 agataatttt tgatttgcag ccgcatcacc aacattatcg gtatgtccgg caagttcaat
     2041 tatcatattt ttttttcgtc gcataagttc tgctaagtta ttaagagatt cgtatgaatc
     2101 tttacgaata cttgttttcc cgacatcaaa gtgaacattt tctaatacaa atactttagg
     2161 taactcatat ttaatagtat aattaaatgt aatttctccg gctacatttg gaataactaa
     2221 ttcattttta gtatcttctg tttcaaaacc ttgaataagc acagcaaatg tttctttagg
     2281 aagtaaaatt tgaaatttac catttgcagt tgaagttcca gaatatattt tacctgtttt
     2341 tttactttta aaacgaacta aatcaccaac cgatggtgaa cctttcatat tggtaatagt
     2401 aacagttacc aaagctaagt cattagttgg ttttaaattt tgtgctgata tacccaaaga
     2461 tactacacat aaacaaagta taaaaaaacg tttcattttg tttgtattta taattttgtt
     2521 gcaatgtaca caaagttata ataaataaaa agagctgtat tgaaaaatac agctcttttt
     2581 ttttgaatag agagagaaaa tcaattaaca caaacgttgg gggaaggaat ggagttcgtg
     2641 ttaatgctga ttattagtta taagcttttt caccatgttc atagtaatca agtccatttt
     2701 cttcttcttt acgacttact ctaattccaa ttaaatattt taatccgtag aataaaatca
     2761 accctgtacc tatagaccaa gctgcaacag cggcaacacc tgttaattga gtaacaatat
     2821 atttaaatcc accaccgtaa aataaaccac cttcaacagc aaatgcacca accataatag
     2881 taccaaaagc accaca
//
LOCUS       DAOTOW010000242         2895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_26883_length_2895_cov_3.391549, whole genome shotgun sequence.
ACCESSION   DAOTOW010000242 DAOTOW010000000
VERSION     DAOTOW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26883_length_2895_cov_3.391549"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2873)
                     /locus_tag="P5243_05285"
     CDS             complement(<1..2873)
                     /locus_tag="P5243_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HRS18895.1"
                     /translation="MIGLAFMGTSGTTYQTALNTAYTNIVGLGNNEAYFSQTLRCITL
                     FMMTGNFWKPGTVVAANPLVTAATTNTAGSKITLTFNKTMATPAAAAYSAFTLRVNGS
                     TVVNAFSAIALNGTSSIDLTLSSPVSLGDVITISYTPGTIQSADANVLEAFTNQSVTN
                     AISGGNTLIADCEDVNQTKLLTFWYSYKTGSSTITPLSSTTSPFTMTLGGANGTDSAA
                     IVTGQLVNPGAPSYESCGIGFAMKDPEANYDLTGATGISFWHKGDAIVFNVILDDAYI
                     KNNAGYDYAISVNAHANWTLVEVPFTNMSQPSWMTTGDATAVANVKPFDASKVTKLQW
                     QIKDGTARSFNYGIDEVTVMGKVLNLPTQTTIDKSLLASAITSATAIHNAAIEGTAVG
                     QYPVGSKATLLNAITAAQTVYNNVSATQTQVDNAKTTLDAAVATFQASVITSVSTNTL
                     IADCEDGNQTKLLTFWYSYNDAAPGGSSIVTPLSTQTSPFTMSLGGANGTDSAAKITY
                     TLMGAAQLGYDPFVGFGFALKNPEAAYDLSGSTGISFYHKGDAVALEVGLSTNTDSDF
                     YTAQISSHANWTLVTIPWTSFAQSPYWGNDVPWDASKVVKFQWKKQAADGTSGTIWID
                     EVRIDGKVLNLPTQTVVNKDALTAKIAEANTTLSVNNSKIKTNPGVGEYPLSAGMALQ
                     NAIAAAEAVNNNTAATQTQVDNATTTLNTAIQTFLASVVTSVAVDKSLLASSLSTAQS
                     IYNTATEGTAVGNYPVGSKATLLNAITAAQTVYNNTSATQTQVDNAKTTLDAAVATFQ
                     ASIITNTVSKTALQTSIATGNNLYVNSIEGTAVGQYPAGSRNTLLLAINAAQAVYTNT
                     SATQIQVDNAKATLDAAIVTFQNSQITSVVVNKSTLVSTISYATGIYNSAVEGTANGQ
                     YPVGSKTTLMNAITAAQAINNNTSATQLQVDNAVSTLNTAVTT"
BASE COUNT          824 a          542 c          517 g         1012 t
ORIGIN      
        1 aaagtagtaa ctgctgtatt taaagtagaa acagcattgt caacttgtaa ctgagtagcc
       61 gaggtattat tatttatcgc ttgagctgca gtaattgcat tcattaaagt tgttttacta
      121 cccacaggat attgtccatt tgcagttcct tctacagcgc tattatatat acctgtagca
      181 tatgaaatag ttgataccaa agtagattta tttacaacca cacttgtaat ttgtgaattt
      241 tggaacgtta caatagcagc atctaatgta gcttttgcat tatctacttg tatttgtgta
      301 gcacttgtat ttgtatatac cgcttgtgca gcattaattg ccaataataa tgtattacga
      361 gaacctgcag gatattgtcc aacagctgtt ccttcgatag aatttacata taaattatta
      421 cctgttgcta ttgatgtttg caatgctgtt tttgacacgg tattggttat aatcgaagcc
      481 tggaatgtag ctactgcagc atctaatgta gtttttgcat tgtcaacttg agtttgtgta
      541 gcacttgtgt tattatatac tgtttgagca gctgtaattg cattcaataa tgttgcttta
      601 gaacctacag ggtaattacc taccgcagtt ccctcagttg ctgtattgta tatagattgt
      661 gcggttgata gagaacttgc aagcaacgat ttatcaactg ccactgacgt tactactgat
      721 gctaagaatg tttgaatagc agtattgagc gttgtagttg cattatcaac ttgagtttgt
      781 gtagctgctg tattattatt aaccgcttct gctgccgcta tagcattttg caatgccata
      841 cccgccgaca atggatattc tcctacaccc ggatttgttt ttattttaga attatttacc
      901 gataatgtag tatttgcttc agcaatttta gcagttaacg catctttgtt tactactgtt
      961 tgagtaggca aattaagtac cttaccatca attctaacct catcaatcca aattgttcca
     1021 gatgttccat cagctgcctg ctttttccat tggaatttaa ccacttttga cgcatcccat
     1081 ggtacatcat ttccccaata gggactttgt gcaaacgatg tccatggtat tgttactaat
     1141 gtccaattag catgtgaaga tatttgtgcc gtataaaagt cactgtctgt atttgttgaa
     1201 agaccaacct ctaaagctac agcatcacct ttatgataaa atgatatacc tgttgaacca
     1261 cttaaatcat aggcagcctc cgggtttttt aaagcaaagc caaaacctac aaacggatcg
     1321 taccctaact gtgcagcccc cattaaagta taagtaattt ttgctgcact atcagtacca
     1381 tttgcacctc ccaaagacat tgtaaatgga cttgtttgag ttgataatgg tgttactatt
     1441 gatgacccac caggagctgc atcattatat gaataccaga atgttagaag ttttgtttga
     1501 tttccgtctt cacagtctgc tataagagta tttgtgctta ccgatgtaat aaccgaagct
     1561 tgaaacgtag ctactgcagc atctaatgta gttttagcat tatctacttg tgtttgtgta
     1621 gcacttacat tgttatatac tgtttgtgca gctgtaattg cattaagcaa tgttgcttta
     1681 gaaccaaccg gatattgacc tacggcagtt ccctctattg ctgcattatg tatagctgta
     1741 gccgatgtta ttgcacttgc taataatgat ttgtcaattg tagtttgtgt cggtaaattt
     1801 aatactttac ccattactgt aacttcatca attccataat taaacgaacg tgctgtacca
     1861 tcttttattt gccattgcaa tttagttact tttgatgcat caaagggttt cacatttgct
     1921 actgctgtag catcacctgt tgtcatccaa ctaggttgag acatatttgt aaacggtact
     1981 tccactaatg tccaatttgc atgtgcattt acagatatag cataatcata tccggcattg
     2041 tttttaatat atgcatcatc taaaattaca ttaaatacta tcgcatcacc tttatgccaa
     2101 aatgatattc cggttgctcc ggttaaatca taatttgctt ctgggtcttt cattgcaaaa
     2161 ccaattccgc atgattcata tgatggtgcg ccgggattca ctaattggcc ggttactatt
     2221 gccgcactat ctgttccatt ggctcctcct aaagtcatag taaagggact tgttgttgaa
     2281 gatagtggag ttatagtact agaaccggtt ttatatgaat accaaaaagt taataatttt
     2341 gtttgattta catcttcaca gtctgctata agagtgttgc caccggatat tgcattggtt
     2401 acactttgat ttgtaaatgc ttcaagtaca tttgcatcag cactttgaat agtacctgga
     2461 gtgtatgaaa ttgtaataac atcacctaaa gaaacaggtg aactaagagt aaggtcaatc
     2521 gatgatgttc cattgagtgc tatagcagaa aatgcattaa caacagtaga accatttacc
     2581 cgaagtgtaa atgcactata cgctgcagct gcaggggtag ccatagtttt attgaaagtt
     2641 aaagtaattt ttgagccggc tgtattcgta gttgctgctg ttactaaagg attagctgct
     2701 actactgttc ccggtttcca aaaattaccg gtcatcataa acaatgtaat acaacgcaat
     2761 gtttgactaa aatacgcttc attatttcct aaacctacta tattggtata tgctgtattt
     2821 aatgctgttt gatatgttgt tcccgaggtt cccataaaag caagacctat cataccaaat
     2881 gttccatttt tatat
//
LOCUS       DAOTOW010000243         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27122_length_2874_cov_4.061369, whole genome shotgun sequence.
ACCESSION   DAOTOW010000243 DAOTOW010000000
VERSION     DAOTOW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27122_length_2874_cov_4.061369"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..>2874)
                     /locus_tag="P5243_05290"
     CDS             complement(31..>2874)
                     /locus_tag="P5243_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS18896.1"
                     /translation="TTTREWVYCHVAKKHESLCSIIPNYYTSISNIIDKLLSKTVEMR
                     YQSAHGILTDLRLALIDLENTGTIHDFEIGLTDFSPLFELPQKIYGREKEITALYNAF
                     EELEIWKTSLVLVHGLSGIGKTTLIGELYKPVIEKNGYFISGKFEEFKINTPYYALIQ
                     AFTGFIQYILSEDDSTIAQWKQLFSEELGENAQLIINVIPDFEKIMGKQNRIEDLAAI
                     ESQNRFIVTFLKFVQCIARNTPSLVLFLDDIQSADATSLEMIKNILSDTNSNSIMIII
                     SYRSQEILRVQGFETVIQKIKDVGIKIYEIPVLELKLEHVIEFLQDIFLAKTKDIREF
                     AELCLQKTHGNPFFIREFLQDLFQKGYIILDEKKGEWSWNINLIRSTAITENVAEILS
                     QRIRGLQAHVQKIILQAACIGSRFDVQTLSIINKQPHKEILLALHEILHAGFIQPTDQ
                     SFTDIFIQTPENSIYRFIHERILSSAYAMVDETTKQKNHYEISSYLLQNLTHKQIDDN
                     IFTIVEHCNKGAKFFPEFEYKKILELNVIAAEKAKKSAAFNLTILYLKEASVFLPQNI
                     WNTDYEFALKYYNSYAEAAYLNGLYAEMDEISQIIFTNYKSITDLVWIYEIRILAYQA
                     RHMQSKAIDEGFSFLNKLGIRISKKAGIPKLLFNLLTTNVFIKNYSAEKIILLPEIKQ
                     KRIQAAMHIMGVLTHPLYDTNPTLFAIIIFKLVRFSLRYGTAPISPLAYMMYGMLTNV
                     MLGKPQKGYEYGKIGLQLLQKIEAKRYWAETSVCYNLGVRIWKEPLKNSIVGLLDDFR
                     ISLETGDIGFAISSLATCTNYQFFAGQSLQTIMDTAFKYKRSLAHIPYQKGMKVMDMQ
                     LQTIYNLQHSNIDTDILNGEYFNEIEHFEQKETVSQVAGAYLLKMMIALYTEKTANAY
                     EYVLESRKYIQSLAGILHYAMYHVYESLILLQQ"
BASE COUNT          935 a          413 c          429 g         1097 t
ORIGIN      
        1 tcctaattgc tattattatt attagtgcta ttattgttgt aataatatta atgattcgta
       61 tacatgatac attgcgtaat gtaaaatacc tgcaaggctt tgtatatatt ttctactttc
      121 taaaacatat tcatatgcat ttgcagtttt ttctgtatat agagcaatca tcatttttag
      181 taaatatgct ccagcaactt gcgatactgt ttctttttgt tcaaaatgtt caatttcatt
      241 aaaatattcg ccgttgagaa tgtctgtatc tatatttgaa tgttgtaaat tatatatagt
      301 ttgtaattgc atgtccatta ctttcatgcc tttttgatat ggaatatggg ctaaagaacg
      361 tttgtatttg aatgcagtat ccattatagt ttgtaaactt tgtcctgcaa aaaattgata
      421 attagtacat gttgcaagag aagaaatagc aaatccaata tctccggttt ctaaactaat
      481 tctaaagtca tctagtagac caacaatact attttttaat ggttctttcc aaattcttac
      541 acctaaatta taacatacag atgtttctgc ccaataacgt tttgcttcta ttttttgtaa
      601 taattgtaaa ccaatttttc catattcgta acctttttga ggttttccta acattacatt
      661 ggtaagcata ccatacatca tgtaagctaa tggcgatata ggagctgttc catagcgtaa
      721 agaaaaacgt accagtttaa aaatgattat agcaaataat gttggattag tatcatacaa
      781 tgggtgggta agaactccca ttatatgcat agctgcttga atacgttttt gtttaatttc
      841 gggtaaaaga ataatttttt cggcgctata gttttttata aacacattag ttgttagtaa
      901 attgaaaagc aattttggta tgcctgcttt ttttgatata cgaattccta atttattaag
      961 aaaagaaaat ccttcatcta ttgctttact ttgcatatgt ctagcttggt aagctagtat
     1021 gcgaatttca tatatccata ccaaatcagt aattgattta taattggtaa atattatttg
     1081 agaaatttcg tccatttcag catataaccc attgagatat gctgcttctg catatgaatt
     1141 ataatatttc aatgcaaatt cataatcagt gttccaaata ttttgaggta aaaatacaga
     1201 agcttctttt aggtataata ttgtaagatt aaaagctgca gatttttttg ctttttcagc
     1261 agctataaca ttaagttcta atattttttt atattcaaat tccgggaaaa atttagctcc
     1321 tttgttgcag tgttctacaa ttgtaaaaat attatcgtca atttgcttat gagttaaatt
     1381 ttgtaataag tatgatgata tttcataatg atttttttgt ttagtcgttt catcaaccat
     1441 agcataggcc gatgataata ttcgctcatg gataaaacga taaatactat tttcaggtgt
     1501 ttgaataaat atgtcggtaa aggattgatc ggtaggttga ataaaaccgg catgtaaaat
     1561 ttcatgcaaa gcaagaagta tttctttatg tggttgtttg ttaattatag atagtgtttg
     1621 aacatcaaat cggcttccta tacaagcagc ttgcaaaatt attttttgta catgcgcttg
     1681 taatccacga atacgttggc tcaatatttc ggcaacattt tcggtaattg cagttgagcg
     1741 aattaaattt atattccacg accattcacc tttcttttcg tcaagaatta tgtatccttt
     1801 ttgaaataaa tcttgcagaa attcacgaat aaaaaatgga ttaccatgtg ttttttgcaa
     1861 acataattct gcaaattctc taatatcttt ggttttagct aaaaatatat cttgcagaaa
     1921 ctcaataaca tgttctaatt taagttctaa tacagggatt tcataaattt ttataccaac
     1981 atctttaatt ttttgaatca ctgtttcaaa tccttgaact cttagaattt cttgtgagcg
     2041 ataagaaata ataatcatta tggaattact atttgtatca gaaagaatgt tcttaatcat
     2101 ttcgagcgat gttgcatctg cagattgaat atcatcaaga aataaaacaa gagaaggggt
     2161 attacgagca atacattgta caaattttaa aaatgttaca ataaatctat tttgagattc
     2221 aattgcggct aaatcttcaa ttctattttg ttttcccata attttctcaa aatcaggaat
     2281 aacattgatt atcaattgtg cattttcgcc taattcttca gaaaataatt gtttccattg
     2341 agcaatagta gaatcatctt cagagagtat gtattgtatg aatcctgtaa atgcttgaat
     2401 taaggcataa taaggggtgt ttattttgaa ttcttcaaat ttacccgaaa taaaatagcc
     2461 atttttttca atgaccggtt tatacaattc tccaataagt gttgttttac caattcctga
     2521 taagccatga acgaggacta atgaagtttt ccaaatttct aattcttcaa aagcattata
     2581 aagggcagtt atttcttttt ctcgtccata tattttttga ggtaattcaa atagaggcga
     2641 aaaatctgtt agtccaattt caaaatcatg tatggtcccg gtattttcta aatcaatgag
     2701 agctaatcgt agatctgtaa gtattccatg agcactttga tagcgcattt ccacagtttt
     2761 tgataataat ttatcgataa tatttgaaat acttgtataa taattgggaa tgatactaca
     2821 gagtgattcg tgtttttttg ctacatgaca atatacccat tctctggtag tagt
//
LOCUS       DAOTOW010000244         2872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27148_length_2872_cov_2.723465, whole genome shotgun sequence.
ACCESSION   DAOTOW010000244 DAOTOW010000000
VERSION     DAOTOW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27148_length_2872_cov_2.723465"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2364
                     /locus_tag="P5243_05295"
     CDS             <1..2364
                     /locus_tag="P5243_05295"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS18897.1"
                     /translation="TVVGNGTTFTTSTAGTYYVKQQNASATCISETTAATLFVKPLPA
                     APQTNNVTVCDGNSGTLTIGTTAKWYQNQSDITALYTGLTYTPVVTQPGTYTYYVSEV
                     LNGCEGPKSLITYTIEAKPTMTINDVIVTVGASVPDLSVVTAAQNTVSWYQSDKTTFI
                     KNGTSYTTGKTAIGLYTYYVEATSPSQCKSAKTAITLQITDCNLAAPTVSTSNTSICV
                     GSPSPTLQATATQNVRWYSDAALTKLVSNSAQFTPADTDPGTYTYYAVQYAACVSPAK
                     AISFTIHALPNPQIFAPSTAKITDQPVTITVSPLGGSLLGTGLQGNTFVPSSVTAGIY
                     NIQYSYTDANQCSKSVIHTIQVIQEQTVDRTQLGDTILRANAIFTQYAENQLYSTAAK
                     IELQNAITIAEFYYTNYLNYNNQIMLEQTLALSQAIKTFLQSYNPVSIDVTALEAKIA
                     EANTLYNQNLSNVGTNPGQYPASSFIELQNQINSAQNKVTNPPATQIEVDNAVIVLQN
                     AIDAFIASKIPTPVVESITTTPTFVKLVVGATHTPQVVFTPSGAVGTIQWVSSNTSVA
                     TVIAGTGVITAKGKGTTSITGTLMENPSKTVTIVVQVSGNPTITSATMNNLGNKIILE
                     FSEPMAEPSANIYQDIQVAGINPYFYTVTNVQKDPSNSNAIILSLGSVIDNPALITVI
                     YSGNSLQSEAGAPVQSFNTKLSVDIEVVDVNILVYPTVTSSKVTLVGVGLADVIKVIS
                     SNGQLVLTQPIHGDTQEIDVTTLAQGTYTVLVMSKSSIIIKSSIIKK"
     gene            2507..>2872
                     /locus_tag="P5243_05300"
     CDS             2507..>2872
                     /locus_tag="P5243_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRS18898.1"
                     /translation="MQQLLEGLNEAQTRAVVNTQGPTLVIAGAGSGKTRVLTYRIAYL
                     LSQGIPSYKILALTFTNKAAKEMKERITALVGAQKAYDLWMGTFHSIFSKILRFESQT
                     IGFNSNFTIYDTQDSKNLIT"
BASE COUNT         1078 a          495 c          421 g          878 t
ORIGIN      
        1 acagttgtag gaaatggtac aacatttaca acaagcacag ccggtacata ttatgtaaaa
       61 caacaaaatg caagtgcaac atgtataagt gaaactacag cagctacatt gtttgtaaaa
      121 ccactaccag cagcaccaca aacaaacaat gttacagttt gtgatggaaa ttcaggtact
      181 ttaacaatag gtactactgc taaatggtat caaaatcaat cagatattac tgctttatat
      241 acaggtttaa cgtatactcc tgttgtaact caacccggaa cttatacata ctatgtaagt
      301 gaagtattaa atggttgtga aggtccaaaa tctttaataa catatacaat agaagctaaa
      361 cctactatga ctattaatga tgtaatagta actgtaggag catcagtacc tgatttatca
      421 gtagtaactg cggcacaaaa tactgttagt tggtatcaat cagataaaac tacattcatt
      481 aaaaatggaa cttcatatac tacgggtaaa actgctattg gattatacac atactacgta
      541 gaagctacca gtccatcaca atgtaaatct gctaaaactg caataacatt acaaattaca
      601 gattgtaatt tagcagcacc aactgttagt acatcaaata caagcatttg tgtaggctca
      661 ccaagtccaa cattacaagc tacagcaact caaaatgttc gttggtattc agatgcagca
      721 ttaacaaaat tagtttcaaa ttctgctcaa tttacacctg ctgacactga cccgggaaca
      781 tatacatact atgctgttca atatgcagct tgtgtaagtc ctgctaaagc aattagcttt
      841 acaatacatg cattgccaaa tcctcaaatt tttgctccaa gtactgcaaa aattactgac
      901 caacctgtta ctattactgt ttcaccactt ggtggtagct tattaggaac cggattacaa
      961 ggcaatactt ttgtaccaag ttctgtaaca gcgggtattt acaatattca atattcatat
     1021 actgatgcta atcaatgttc taaatctgtt attcatacaa ttcaagttat tcaagaacaa
     1081 acagttgata gaactcaatt aggtgataca attttacgtg ctaatgctat ctttactcaa
     1141 tatgctgaaa atcaattata cagcacagct gctaaaattg aattacaaaa tgcaattact
     1201 attgcagaat tctactatac taattactta aattataaca atcaaataat gttagaacaa
     1261 acattagcat tgagtcaagc tattaaaaca ttcttacaaa gctataatcc tgtaagtatt
     1321 gatgtaacag ctttagaagc taaaatagct gaagcaaaca cattgtacaa tcaaaattta
     1381 agtaatgtag gaacaaatcc aggtcaatac cctgcaagca gttttattga attacaaaat
     1441 caaattaatt cagctcaaaa taaggtaact aatcctcctg ctactcaaat cgaagttgat
     1501 aatgctgtaa ttgtattaca aaatgcgatt gatgccttta tagcttcaaa aataccaact
     1561 cctgttgttg aaagtatcac tactacacct acatttgtta aattagtagt aggagcaact
     1621 catactccac aagtagtttt cacaccaagt ggagctgtag gaaccattca atgggtaagc
     1681 agcaatactt cggtagcaac tgtaatagca ggtaccggtg taataacagc aaaaggaaaa
     1741 ggtacaacat ctattactgg tacattaatg gaaaatccat caaaaactgt aacaatagtt
     1801 gttcaagtta gtggaaatcc aacaattaca tctgctacta tgaataattt aggtaacaaa
     1861 attattcttg aattcagtga accaatggca gaaccatctg caaatattta ccaagatatt
     1921 caagttgcag gtatcaatcc ttacttctat acagtaacta atgttcaaaa agatccaagc
     1981 aatagtaatg caattatact ttcattaggt tctgtaattg acaatcctgc tttaatcaca
     2041 gttatatata gcggaaattc attacaatct gaagcaggtg cacctgtaca atcgtttaac
     2101 acaaaattat cggttgatat agaagtggtt gatgtaaata ttttagtata cccaactgta
     2161 acatcttcta aagtaacatt agtaggtgtt ggattagcag atgtaataaa agtaattagt
     2221 tctaacggac aattagtatt aacacaacca attcatggtg acacacaaga aatagatgtt
     2281 acaacattag ctcaaggaac atatactgtt cttgtaatgt caaaaagttc aataatcata
     2341 aaatcttcaa ttattaaaaa ataatagcag atttttataa taaattcaaa aaaagggtac
     2401 acagtatagt gtaccctttt tttgtttata acttataatg tagttagtaa gtatattata
     2461 atgcatatct tatttttgca tattctataa tttatttcaa taatacatgc aacaattatt
     2521 agaagggctt aatgaagcac aaacaagagc agtagttaat acgcaaggtc ccactttagt
     2581 aatagcaggt gccggttccg gaaaaacaag agtattaaca tatcgtattg catatttact
     2641 ttcacaagga atcccttctt ataaaatatt agctttaaca tttacaaata aagccgcaaa
     2701 agaaatgaaa gaacgtatta cggctttagt tggagctcaa aaagcatatg atttatggat
     2761 gggaactttt cactctatat tttcaaaaat tcttcgtttt gaatctcaaa ctataggatt
     2821 caactctaat tttacaatat atgacactca agattctaaa aatcttatta ct
//
LOCUS       DAOTOW010000245         2869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27195_length_2869_cov_2.545131, whole genome shotgun sequence.
ACCESSION   DAOTOW010000245 DAOTOW010000000
VERSION     DAOTOW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27195_length_2869_cov_2.545131"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="P5243_05305"
     CDS             complement(<1..722)
                     /locus_tag="P5243_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HRS18899.1"
                     /translation="MKTIGTFMKVAFAVICIVGFTSVFAGPPTWTRVDYTNTTAFVGE
                     VQINAYDAGFPVTVEAGDYIGAFVNGECRMIAQIFTYNGKLYVSSVIHGGDLFDGFPS
                     NDPSITNSQELIEFRVWDNSASAEINRTVKGTLKATPGGEVLDYIIGKPNTDSKLASL
                     SVTGITLTPAFSASTTAYTVSLPAGSSLPALAAYTATVADSRATVTVTPATNFTTGNI
                     TTIVVTAEDGTKTTYTITFSEQA"
     gene            complement(1019..2179)
                     /locus_tag="P5243_05310"
                     /pseudo
     CDS             complement(1019..2179)
                     /locus_tag="P5243_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
     gene            complement(2239..>2869)
                     /locus_tag="P5243_05315"
     CDS             complement(2239..>2869)
                     /locus_tag="P5243_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18900.1"
                     /translation="PQETSNNDYVEKKKNELPLHKKIIKTTFILSPVLFAAILIPNII
                     HAPQMTGIISLFRDTEAYVDFSIPQKPMPILSYKKIEQIEYTPQQENNTIPEPKETNN
                     VSNNNSKEQQKNNSIVSIPKTQESYNNSKSQNVNLYIIVGSYSSETNAQVFLNTIKEQ
                     YTQAGILIKDGKIRVFIEKNSNKELTLHALRSIKQKDEFKNAWIYTERT"
BASE COUNT          914 a          438 c          391 g         1126 t
ORIGIN      
        1 gcttgttcac taaatgttat tgtatatgtt gttttagtac catcttctgc agttacaact
       61 attgttgtaa tatttcctgt agtaaaattt gttgcaggcg ttaccgttac ggtagctctt
      121 gaatcagcaa ctgtagcagt gtaagcagct aacgcaggta atgaacttcc tgcaggtaat
      181 gaaactgtat atgcagtagt agaagcacta aatgcaggtg ttaaagtaat tcctgtaaca
      241 cttaacgaag ccaatttact atcggtattt ggcttaccta ttatataatc taaaacttct
      301 cctcccggag tagcttttaa agtacctttt acagtacgat ttatttctgc tgatgctgaa
      361 ttatcccaaa ctctaaattc aatgagttct tgagaatttg taatactagg gtcatttgaa
      421 ggaaatccat caaataagtc accaccatgt atcactgatg atacatataa tttaccatta
      481 tatgtaaaaa tttgagcaat cattctgcat tctccattta caaatgcacc aatgtaatca
      541 ccagcttcaa ctgtaacagg aaaaccagca tcatatgcat ttatttgaac ctcacctaca
      601 aatgctgtag tattagtata atctacacgt gtccatgtag gaggacccgc aaatacagat
      661 gtaaacccta ctatgcagat aactgcaaaa gcaactttca tgaatgtacc gatagttttc
      721 ataaatatgt attttaaatt attttacgtt tcttttgaat ttataaaaat agagaaaaaa
      781 tctcaattac aaaaataagt atatatattt attattatag tataaaattc gtgcaaaata
      841 agggtaaatt tgttgcatgt atattaaatt ttaatatgta aaataatgat aatgtgtact
      901 ttatataaat aatatcttat tgtttcaaat acaattatta gacaatttat tgcaaataat
      961 ccctattatg ataggagtta aaaaaaagac tcttataatt tataagagtc tttttttatt
     1021 actttttatt tgttaatatt ttggagagtt cgacatcgag agttgtgttg ctattttttt
     1081 cggaatccca tcctctaatt ccaaaaagcc ctttgtagtc ccatgcagaa tagggaatat
     1141 tatatttttc aaaaactgat actatatctt tataatattg caatctgtca tttctttttg
     1201 tgtaaggtaa tgcaccaaat tcgccacaat ataattgtaa ttttaattta cgagcttttt
     1261 ctatagcagg cataaatatt ttctcaaaac gttcaggtcc atataattct aaagcatcat
     1321 caaaagcatg ttctccttcg ttttttgcta aatgtttatt acgttcatat acttcttttg
     1381 taataattcg tcccggatat tgtaccgaat cattaaaatc tttatatgct gtccaatttg
     1441 ccttataatg tgtaaaaagt aatggatcgt agttatgaaa acttaaaata atatttttat
     1501 cgtttgcagg tacttttaaa tcaggaaaag tctttgttat ttgccacatg ttagacccaa
     1561 caataataac tctctccggt tcttttgctc taattgtttt aataccgcct tcaattaatt
     1621 tattccagtc atctgggttt tcagcaacgg cttcatttaa taattcatat gctaacatag
     1681 attcaggata tcgcttcaaa gaatctgata atgtaatcca tagagaataa aatttttgct
     1741 attctttttc gtctgtaaat aatgtgttgt tgtgaccttc gttaattgca ttaaagtaaa
     1801 aacttcttat aatatgtaaa tcaacaatta ctttcatatc atattccata caccattcta
     1861 tcgcttttcg caaataactg aatgattctg caataggttt tcctgtttcg tcccacattt
     1921 ctttttcatc tataggtaaa cgaatatgat caaatcctaa actgtcaagt aatcgtatgt
     1981 cgtcttttgt aaaaaacaca tctcgttttg aaaaaccata tacttgagac aaccaatgac
     2041 ttatatttat acctctatga attacaaatc ctgaaggatt tataatatct gttgattgtt
     2101 catttttagt tgatttatca tttgagttgc atgaaaacag cacaatgaca ctgataaata
     2161 aaatacattt ttgtaacata attccccctt tttaattttt gagcatcaaa cgtatgcaaa
     2221 ttataattat tgtttttttt acgttctttc tgtataaatc caagcatttt taaattcatc
     2281 tttttgtttt attgaccgca aggcatgtaa agttaattct ttattgctgt ttttttcaat
     2341 aaatacccga atttttccat cttttattaa aatacctgct tgagtatact gttctttaat
     2401 tgtatttaaa aatacttgtg catttgtttc agaactataa cttccaacta taatatacag
     2461 attcacattt tgactcttac tattattata agattcttgt gtttttggaa tagaaacaat
     2521 agaattattt ttttgttgtt ctttagaatt attatttgaa acgttattag tttcttttgg
     2581 ttccggaata gtattgtttt cttgctgtgg cgtgtattct atttgttcta tttttttata
     2641 tgatagaatt ggcattggtt tttgaggaat actaaaatct acatatgctt ctgtatcacg
     2701 aaataaagat ataatacctg tcatttgtgg agcatgtata atattaggaa ttagtattgc
     2761 tgcaaataaa acaggagata atataaatgt tgtttttatt atttttttat ggagcggtaa
     2821 ttcatttttt tttttctcta cataatcatt gttgcttgtt tcttgtggt
//
LOCUS       DAOTOW010000246         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27582_length_2839_cov_2.659483, whole genome shotgun sequence.
ACCESSION   DAOTOW010000246 DAOTOW010000000
VERSION     DAOTOW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27582_length_2839_cov_2.659483"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="P5243_05320"
     CDS             <1..491
                     /locus_tag="P5243_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18901.1"
                     /translation="SKWKFVSLQHSCISGLECVVNKRVVARLGIVKSAVSKHFDIEKP
                     CMYAELYWDTLMQLYGTPIIYKEIPQFPEVRRDLALLLDAHIEFETIKKLAFETERNL
                     LKHMQVFDVYQGKGIPEGKKSYAISFVLQDKEKTLTDKQIEGVMTKLIAKFKNSIGAE
                     LR"
     gene            complement(483..962)
                     /locus_tag="P5243_05325"
     CDS             complement(483..962)
                     /locus_tag="P5243_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRS18902.1"
                     /translation="MRNRLDLDIKRLENMHSSHMQCSKACYHCCMNFGILPIEAFAIA
                     QTFSNKTAFTKTETNEQCIFLIHNLCSIYEHRPFICRTQGLPIVYEVDNQYQLSVCER
                     NFTSQPDSLFTPDNCLFMDDYNNELYALNIEFLKHNPQLGLQYDTLIKVNNCITILS"
     gene            1247..>2839
                     /gene="dnaK"
                     /locus_tag="P5243_05330"
     CDS             1247..>2839
                     /gene="dnaK"
                     /locus_tag="P5243_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRS18903.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNSEGKRTTPSVVAFVE
                     NGERKVGDPAKRQAITNPEKTINSIKRFMGETFDEVQNEIPRVPYKVVRGDNNTPRVQ
                     IDDRKFSPQEISAMVLQKMKKTAEDYLGQTVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LTVRRIINEPTAAALAYGLDKKHKDMKVAVFDLGGGTFDISILELGDGVYEVRSTNGD
                     TQLGGDDFDQVIIDWLAEEFKKSEGPDLRKDPMALQRLKEAAEKAKIELSSSTTTEIN
                     LPYIMPVDGIPKHLVRNLTRAQFEQLADKLIQAVIPPCAQALKDANLTASEIDEVILV
                     GGSTRIPIIQEKVQQFFGKVPSKGVNPDEVVAIGAAIQGGVLAGDVKDVLLLDVTPLS
                     LGIETMGGVMTKLIEANTTIPTKKSETFSTAADNQPSVEIHILQGERSMARDNKTIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILHVSAKDKATGKEQSIRIEASSGLSEDEIKR
                     MRDEAKANEAADREARERIDKLN"
BASE COUNT         1023 a          419 c          549 g          848 t
ORIGIN      
        1 tatcaaagtg gaagtttgta tcattgcaac attcatgtat aagtggttta gaatgtgttg
       61 taaacaaacg tgtagtagct cgtttgggta ttgtaaaatc tgccgtaagt aagcattttg
      121 atattgaaaa accatgtatg tatgccgaac tgtattggga tacacttatg caactttacg
      181 gaaccccaat tatatataaa gaaatacctc aattccctga agttcgtcgt gatttggcgt
      241 tgttgttaga tgctcatatt gaatttgaaa ctattaaaaa acttgcgttt gaaaccgaac
      301 gaaatttatt aaaacatatg caagtttttg atgtatatca aggaaaaggt attcccgaag
      361 gaaaaaaatc ctatgcgatt tcatttgtat tgcaagataa ggaaaaaacc cttaccgata
      421 aacaaataga aggggttatg acaaaattaa ttgcaaaatt taaaaattca ataggcgctg
      481 aattacgata atattgtaat acagttattt accttaatca atgtgtcgta ttgtaaccct
      541 aattgaggat tatgttttag aaattcaatg tttaatgcat ataattcatt gttatagtca
      601 tccataaata agcaattatc aggagtaaac aatgaatcag gttgagaagt gaagtttcgt
      661 tcacataccg aaagttgata ttgattgtca acttcatata caataggtaa cccttgtgtt
      721 ctgcatataa acggacgatg ttcgtatata gaacaaagat tgtgtattaa aaatatacat
      781 tgttcatttg tttcggtttt tgtaaatgcc gttttattag aaaaagtttg agctatagca
      841 aatgcctcta ttggcaaaat tccaaaattc atacagcaat ggtaacatgc ttttgaacat
      901 tgcatatgtg atgaatgcat gttttctaac cgtttaatgt ctaaatctaa acggtttcgc
      961 aaagaaatat aatgtgagat aaattcctta ataatcatag attcataaaa tagttacaaa
     1021 tatagtactt aaaagtacat tgtatagtat ttaccataat tgtattacgt gtgcatttat
     1081 aaatagtttg atactttttt tgtatgtcga aatgtcataa attatgacat ggtttaatta
     1141 attattgagt gcgccgtgac acaattacat agaaattact aaaaaacagc atggcataat
     1201 gcttgaaaaa cgttgaaaaa aatgaacatt ttaaaacaaa ataacgatgg gaaaaatcat
     1261 tggaatagat ttagggacta caaactcgtg tgtttctgta atggaaggaa acgaaccagt
     1321 tgtaataccc aatagcgaag gtaaacgtac aacaccttcg gtagtggcgt ttgttgagaa
     1381 cggcgaacgt aaagtaggag atcctgctaa acgacaagca attacaaatc ctgaaaaaac
     1441 gattaattca attaaacgtt ttatgggaga aacgtttgac gaagtacaaa atgaaatacc
     1501 acgtgtgccc tataaagtag ttagaggcga taataatacg ccacgagtac agatagacga
     1561 caggaaattt tctccacaag aaatttctgc aatggtactt caaaaaatga aaaaaacagc
     1621 tgaggattat cttggacaaa cagttacaga agctgtaatt acagtgcctg catattttaa
     1681 cgatgcgcaa cgtcaagcga caaaagaagc tggtgaaata gcaggtttaa cagtgcgtag
     1741 aattataaat gaacctacag ccgcagcttt agcatatggt cttgataaaa aacataaaga
     1801 catgaaagta gctgtgtttg accttggtgg tggaactttt gatatttcaa tacttgaatt
     1861 gggtgatgga gtatatgaag ttcgttctac caatggtgat acacagctag gtggtgatga
     1921 ttttgatcaa gtaattatag attggttggc agaagaattc aaaaaatctg aagggcctga
     1981 tttacgtaaa gatccaatgg ctttgcaacg attaaaagaa gctgccgaaa aagcaaaaat
     2041 tgagctatca agttcaacta ctaccgaaat taatttgcca tatataatgc ctgtagatgg
     2101 tattcccaaa cacttagtgc gtaatttaac tcgtgctcaa tttgaacaat tggcagataa
     2161 attaattcaa gccgtaattc cgccatgtgc tcaagctctt aaagatgcta atttaacagc
     2221 ttcagaaatt gatgaggtaa tattggtagg tggttctaca cgtattccga ttattcaaga
     2281 aaaagtgcaa caattttttg gaaaagtacc gtcaaaagga gtaaatcctg atgaagttgt
     2341 agctataggt gcagctattc aaggaggagt gcttgctggt gatgtaaaag atgtattgct
     2401 cttagatgtt actcctttat cgcttggaat tgaaaccatg ggtggggtaa tgacaaaatt
     2461 aatagaggca aacactacaa tacctactaa aaaatctgaa acattctcta ctgctgcaga
     2521 caatcaacct tcggtagaaa ttcatatttt acaaggtgaa cgttctatgg ctcgcgataa
     2581 taaaactata ggaagattcc atttagacgg aattccacca gctccacgtg gtgtgcctca
     2641 aattgaagtt acctttgata ttgatgctaa tggtatttta cacgtatcgg ctaaagataa
     2701 agctaccgga aaagaacaaa gcattcgtat agaggcatct tcaggtttga gcgaagatga
     2761 aattaaacgt atgcgtgacg aagcaaaagc taatgaagct gccgatagag aagcaagaga
     2821 acgtatagat aaattaaat
//
LOCUS       DAOTOW010000247         2832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27670_length_2832_cov_3.573641, whole genome shotgun sequence.
ACCESSION   DAOTOW010000247 DAOTOW010000000
VERSION     DAOTOW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27670_length_2832_cov_3.573641"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..539)
                     /locus_tag="P5243_05335"
     CDS             complement(126..539)
                     /locus_tag="P5243_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18904.1"
                     /translation="MFDSREYEFADLTLVLGGKDITGFRGIKYTVKQEKEVVYGKGNE
                     PLKIQKGNKSYEGELTLLQSELETLIANGGGSVLDLQVDAVVAYGNPSKGDALITDVI
                     QGIQFTEEAKELKQGQKYMEITLPFIYLRKKSQVA"
     gene            complement(557..1756)
                     /locus_tag="P5243_05340"
     CDS             complement(557..1756)
                     /locus_tag="P5243_05340"
                     /inference="COORDINATES: protein motif:HMM:NF022218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HRS18905.1"
                     /translation="MLPRVKIYYENGALGSVAPSEDGVIGIVASGTAVADKFALGTAY
                     LITKLSDLDALGITEGSADVNKHIYKCVKEIYSIAQEGSKVWLQACANTVTMTDMFDK
                     TKTYAKTLLDATNGAINILVSCKQEPTEYTPTIVDGLDGDVYTAMAKAQELAVWSAEE
                     KYAPLFVILEGRRYAGTASSLRDLHLGANNRVQILVADTEASSNTACVGLLAGTYANV
                     PVHRSAARVKNGALPAIIYIGTNKAELGSPDVIHDAGFVTARTFIGKAGYFWTDDKLA
                     TAVSDDYALVPRRRVIDKAYRIAYATILEEIGDEMPVTDDGKIPAAIAKSIQNKVETA
                     IENTMTVNGELGVDPGNAKDTGVKCFIDINQNVVSTSKFIASLQVKPYGYTKYIDVYL
                     GFQTTSN"
     gene            complement(1762..2124)
                     /locus_tag="P5243_05345"
     CDS             complement(1762..2124)
                     /locus_tag="P5243_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18906.1"
                     /translation="MTKEQKRARQCVCLGVEFTPELKAMSDKSFADFVKAKKAEKIPE
                     VPSQTKTDEQNAVSSTPAAPTQKMSKHQEKAMEIMRDHDCDEVFRTSDGYWFTERYAA
                     EMHNRIAKGELEVFTSKK"
     gene            complement(2121..2573)
                     /locus_tag="P5243_05350"
     CDS             complement(2121..2573)
                     /locus_tag="P5243_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18907.1"
                     /translation="MKLLNKITLIVFVIFLASCNRLMHEPVITQTYIEKLVPVAVPAD
                     SSYMTALFECDSTNKVIMTQLSEAKSARMETSYVYSNGTLTYKTKTIHDTVYVPKTII
                     QTFVEKPVDRIVEVNVLKWWQKTLIYMGVLMLLYTIWKIVVIIKNIYV"
     gene            complement(2557..>2832)
                     /locus_tag="P5243_05355"
     CDS             complement(2557..>2832)
                     /locus_tag="P5243_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18908.1"
                     /translation="QEAIKTYRELAEDLRAELKASNDDRAALREELEASKKERENTLT
                     ELNKVKNELERLTKINKRICALLEKIEPENYSQIIDEIKKQINETTK"
BASE COUNT          735 a          616 c          428 g         1053 t
ORIGIN      
        1 cgatttttac ttttttcatt gttatgctat ttttatatat tatttaaact atatttaaaa
       61 agtatcggtc tgtcccgaat gccaaccgtc tatccgattt gtctctgccc atgaaaaaag
      121 tatctttagg ctacttgcga tttttttctc aagtaaataa acggaagtgt tatttccata
      181 tatttctgac cttgcttcaa ttcttttgcc tcttcggtaa actgtatacc ttgtatcaca
      241 tcggtaatca aagcatcacc cttgcttgga ttaccatatg caactaccgc atctacttgt
      301 aaatccaata cgctaccacc accattagct attaatgttt ccagttccga ttgtaatagt
      361 gtaagttctc cttcgtatga tttattacct ttttgtattt tcaatggctc gttgcctttt
      421 ccgtatacaa cttccttttc ttgtttcacg gtatatttta ttccgcgaaa tcctgttata
      481 tctttgccac ccaacactaa ggttaagtct gcaaattcgt attctctact gtcaaacata
      541 ttgtacgttt ttatgattaa ttactcgttg tttgaaaacc caaatacaca tctatgtatt
      601 ttgtatagcc atacggcttc acttgtaagc ttgcaataaa cttcgatgta gacactacgt
      661 tttgattaat atcaataaaa cattttacac cggtatcttt tgcattaccc ggatctacac
      721 caagctcacc atttaccgtc atagtatttt ctatagccgt ctctactttg ttttgtatag
      781 atttagctat tgctgcaggt attttaccat cgtcggttac aggcatttcg tcgcctattt
      841 cttcaagtat tgtggcatat gctattctat atgctttgtc tattactcta cggcgtggta
      901 ctagagcata atcatcactt actgcagtag caagtttatc atcagtccaa aaatatcctg
      961 cttttcctat aaatgtacga gcagtaacaa atcctgcatc gtgtattaca tcgggacttc
     1021 caagctctgc tttgtttgta cctatataaa tgattgcagg caaagcaccg tttttaacac
     1081 gtgctgccga acgatgtaca ggaacattag catacgtacc tgcaagtagt ccaacacatg
     1141 ccgtattgct cgaagcttcc gtgtcagcaa caagtatttg tacacggttg tttgcaccca
     1201 aatgcaaatc acgaagcgac gaagctgtac ctgcgtatcg acgtccttct aatattacaa
     1261 acaatggagc atatttctct tcagcactcc atactgcaag ttcttgtgct tttgccatgg
     1321 ctgtatacac atctccatca agtccgtcta ctatagttgg agtatattcc gtaggttctt
     1381 gcttgcaact caccaatata tttattgcac cattggtagc atcaagcaat gttttagcat
     1441 aagtttttgt tttgtcaaac atatcggtca ttgttaccgt atttgcacaa gcttgtaacc
     1501 atactttact accttcttgg gcaatagagt atatttcttt tacacattta tatatgtgtt
     1561 tatttacatc agcagaacct tctgttattc ccaatgcatc taaatcgctc aatttagtga
     1621 taagatacgc agtccccaat gcaaacttgt cagctactgc tgttcctgaa gcaactatac
     1681 caatcactcc atcttctgat ggagctaccg agcccaatgc tccattttca taataaattt
     1741 taacgcgtgg taacatatat tttatttttt agaggtaaat acttccaatt ctcctttggc
     1801 tatacgatta tgcatttctg ccgcataacg ctctgtaaac cagtaaccgt cagaagtcct
     1861 aaatacttca tcacaatcgt gatctcgcat tatctccata gctttttctt ggtgtttgct
     1921 cattttttga gtaggagcag ctggagttga tgaaacggca ttttgctcat cagtttttgt
     1981 ttgtgatgga acttcgggta ttttttctgc tttttttgct ttcacaaaat cagcaaacga
     2041 tttgtcgctc atagctttaa gttcaggagt aaactctaca cccaaacaaa cacattgccg
     2101 tgctcgcttt tgttcttttg tcatacgtat atgtttttaa ttattactac tattttccat
     2161 atggtataca atagcatcaa cactcccatg tatatcaatg ttttttgcca ccacttcaat
     2221 acgtttacct ctacaattct atccacgggt ttttccacaa atgtttgtat tattgtttta
     2281 ggaacataca ccgtgtcatg tattgtttta gttttataag tcaatgttcc gttactatac
     2341 acataagaag tctccatacg agcactctta gcttcactca gctgtgtcat tattacctta
     2401 ttggtactat cgcactcaaa caatgctgtc atgtacgaag agtcagcagg tacagcaacg
     2461 ggaacaagtt tctctatata tgtttgagtt atcaccggct cgtgcatcaa cctgttgcac
     2521 gacgcaagaa atatcacaaa tactatcagt gttattttat ttagtagttt cattgatttg
     2581 tttttttatt tcatctatta tctgactata attttccggc tcaatttttt cgagcaaagc
     2641 acatattcgt ttattgattt ttgttagtct ctctagttcg tttttaactt tatttaattc
     2701 ggtcaaagta ttttcacgtt cttttttact cgcttccaat tcttctctca aagctgctct
     2761 gtcatcgttt gacgccttaa gctctgcacg taaatcttcc gctaattctc tatacgtctt
     2821 tattgcttcc tg
//
LOCUS       DAOTOW010000248         2825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27766_length_2825_cov_2.650542, whole genome shotgun sequence.
ACCESSION   DAOTOW010000248 DAOTOW010000000
VERSION     DAOTOW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27766_length_2825_cov_2.650542"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="P5243_05360"
     CDS             <1..801
                     /locus_tag="P5243_05360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS18909.1"
                     /translation="NPHTFTINTSLIEQAITPRTKAIMPVHLFGQAANMNHIMEIAER
                     HNLYVIEDCAQCFGANYIVKGEQKKLGTIGTIGCTSFFPSKNLGCFGDGGAIFTNDSE
                     LAVIIKKIANHGSQKKYYHSIIGVNSRLDGIQAAILRVKLKYIDIYNASRQQAAQWYN
                     QRLKDINWITTPQLEPYSTHVYHQYTIILQDVSNTELQTFLQTHGIPSMIYYPVPMHK
                     QEALQIFNPQQCPHSEFASKHVLSLPMHTELTESQVDFICNCITLFKP"
     gene            815..1396
                     /locus_tag="P5243_05365"
     CDS             815..1396
                     /locus_tag="P5243_05365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS18910.1"
                     /translation="MSQKNYYAHETAIIDSGCQIGSNTKIWHFSHIMPNCIIGERCNI
                     GQNVVISPDVVLGTNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVLNPRSAIVRKSEY
                     KQTLVKKGASIGANATIVCGMTIGEYAIIGAGAVVTKDIKPYALVIGNPARQTGWVSE
                     YGHKLQFDKNNKAICPESSQEYILENDLVKRIK"
     gene            1404..2375
                     /locus_tag="P5243_05370"
     CDS             1404..2375
                     /locus_tag="P5243_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRS18911.1"
                     /translation="MKISKILDGFILALISVIILAWLFPNIGTNTQYVDLKKISEYGI
                     TIIFFFYGVKLSPQKIREGLSNWKMHIVIQITTFIIFPILVLITKPFFTDIHIWLSIF
                     FVAALPSTVSSSVVMVSLAKGNVPGAIFNATISSLGGIFISPLILSFFIVQSNIEINT
                     SEILIKLLFQILVAMIAGIVLHSKLKIFAEKQSKRLKKLDQSIVLLIVYTSFCTSFES
                     GIFNTIALSTIVFIFIGIGFLFLIVFVILKKISSFFDFSYEDTVTTIFCGSKKSLIHG
                     VVLSKVLFSTTILSGFILVPIMIYHSLQLIAIGFLIPKMQTIIMKKK"
     gene            2459..2803
                     /locus_tag="P5243_05375"
     CDS             2459..2803
                     /locus_tag="P5243_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18912.1"
                     /translation="MMKKFILILISSFMFLTHQAQEMTEIVNQSTNKSLHILPRYFND
                     NIIVSNTSDIETKCELYIFSSTNGRNVLSKQLQIESSFYKVPTNSLKKGEYIYFIVEN
                     NKPIARGTFIKS"
BASE COUNT         1058 a          431 c          393 g          943 t
ORIGIN      
        1 aacccacata catttacaat aaacacatcg ctcattgaac aagccataac accacgaaca
       61 aaagcaataa tgccggttca cttatttgga caagcagcaa acatgaacca tattatggaa
      121 attgccgagc gccataattt gtatgtaata gaagattgtg cacaatgttt tggagcaaat
      181 tatatagtta agggggaaca aaaaaaatta ggtaccattg gaactattgg ttgtacgtca
      241 tttttccctt ctaaaaattt aggatgtttt ggtgatggcg gagcaatatt tacaaatgac
      301 agtgaactag cggttatcat aaaaaaaata gctaatcatg gttctcaaaa aaaatattac
      361 cattctataa taggagtaaa ttcaagatta gatggaatac aagctgctat attgcgtgta
      421 aagttaaaat atatagatat ttataatgct tcgcgacaac aagcagctca atggtataat
      481 caacgactta aagatattaa ttggataaca acacctcaac tagaaccgta ttcaacccat
      541 gtataccatc agtatacaat tatactgcaa gatgtttcaa acacagaatt acaaacattt
      601 ttacaaacac atggtatacc tagcatgata tattaccctg ttcctatgca taaacaagaa
      661 gcattacaga tttttaatcc acagcaatgt ccacattcag aatttgcatc aaaacatgta
      721 ttaagtttac ctatgcatac cgaacttacc gaatcacaag ttgatttcat atgtaattgt
      781 attacattat ttaaaccata aataattgca taaaatgtct caaaaaaact attatgctca
      841 cgaaaccgca ataatcgaca gcggttgcca aattggttcc aacactaaaa tttggcattt
      901 ttctcatatt atgcctaatt gtataatagg tgaacgatgt aacattggac aaaatgtagt
      961 aatatctccc gatgttgtat taggcaccaa tgtaaaagtt caaaataatg tatcaatata
     1021 tacaggagtt atttgtgaag acgatgtgtt tttaggtccg tctatggtgt ttacaaatgt
     1081 attgaatcct agaagcgcta ttgtaagaaa gtcggaatac aaacaaactt tagtaaaaaa
     1141 aggtgctagt ataggagcaa acgcaacaat agtttgcggt atgacaattg gagaatacgc
     1201 tatcattggt gcaggtgctg ttgtaactaa agatataaaa ccctacgcct tagtaatagg
     1261 aaatcctgca cgtcaaaccg gttgggtgag tgaatacgga cataaattac aatttgataa
     1321 aaacaacaag gctatttgtc ccgaaagttc acaagaatat attcttgaaa acgatttggt
     1381 aaaacgaatt aaataatctg cttttgaaaa tttcaaaaat tcttgatggt tttattcttg
     1441 cacttattag cgttataata cttgcatggc tattccctaa tattgggaca aacactcaat
     1501 atgtggattt aaaaaaaata tctgaatatg gtatcacaat cattttcttc ttttatggag
     1561 ttaaattaag tcctcagaaa atacgagaag gcttatctaa ttggaagatg catattgtaa
     1621 tacaaatcac aacattcata atatttccaa tacttgtttt aatcactaaa ccttttttta
     1681 cagatattca tatttggctt agtatatttt ttgtagcagc acttccatca acagtttcat
     1741 catcggttgt tatggtttct cttgcaaaag gtaatgtgcc cggagctatc tttaatgcta
     1801 caatttcaag tttaggaggt atatttattt caccactaat tcttagtttt tttattgtac
     1861 aatcaaatat tgaaattaat acaagtgaaa tactcataaa attattgttt cagatattag
     1921 ttgcaatgat tgcaggcatt gttttacata gcaaactaaa aatatttgca gaaaaacaat
     1981 caaaaagatt aaaaaaactt gaccaaagca tagtacttct tattgtttac acatcgtttt
     2041 gtacttcatt tgaaagtgga atatttaaca ctatcgctct ttctactatt gtatttattt
     2101 ttataggtat aggatttctg tttctcatag tttttgtaat tctaaaaaaa attagctcat
     2161 tttttgattt ttcatacgaa gataccgtta caacaatatt ttgcggatca aaaaaatcat
     2221 taattcatgg tgtagtttta tccaaagtac ttttttcaac aacaatatta tccggcttta
     2281 tattagttcc aataatgata tatcactcat tacaacttat tgcaatagga tttcttattc
     2341 caaaaatgca aactataatt atgaaaaaaa aataaatcaa aaaaatctat cttttttaga
     2401 acttatttat tatctttacg taattgattt attttattta tttcataaca tatagattat
     2461 gatgaaaaaa tttattctta tactcatttc aagttttatg tttttaacac atcaagcaca
     2521 agaaatgaca gaaattgtta atcaatcaac aaataaatca ttgcatattt tacctcgata
     2581 ttttaatgat aatataattg tatcaaacac aagtgatatt gaaaccaaat gtgaattata
     2641 tatattttcc tcaacaaacg gaagaaatgt attatcaaaa caattacaaa tagaatcaag
     2701 tttttataaa gttccaacta actccttaaa aaaaggagag tatatatatt tcatagttga
     2761 aaataataaa ccaattgctc gaggaacatt cattaagagt taaatcaata aactaaaaaa
     2821 ttcct
//
LOCUS       DAOTOW010000249         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27861_length_2819_cov_3.476845, whole genome shotgun sequence.
ACCESSION   DAOTOW010000249 DAOTOW010000000
VERSION     DAOTOW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27861_length_2819_cov_3.476845"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..2349
                     /locus_tag="P5243_05380"
     CDS             97..2349
                     /locus_tag="P5243_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS18913.1"
                     /translation="MRGIFVTTIGFLMYVCIYAQAPNTWKDWYSYNSVLSIESIDGTL
                     YALCSNGLFTYNPSKGQITKISKIQGLSDVDLTTMKYNEEAKKLVIGYTNGIIDILSF
                     PGQKITSIPTIAKRQLYGSKAIRDIVFKHDTAYIATDFGLVTMNLQNNEFIHTAVLGN
                     QGESVGVNAVTIDYTNAILFAATQKGIFSIGLHQNISDGFLWKHVPIGVHQFSKITHI
                     LYYNNVMYYSAAQNNWEQYDTIYSYSNSNTSVFDKVIYLEKITSYSQNLAIVSRRQIK
                     LYNNGELLNIITNTVQEVNFSGICNCNNALWVSDYAKGIYSIENYKSYYPQGPYSNIT
                     SEVFCRNNAVHLVCGSSSAYQIGQYNVLVNGTWYGHVNWNVKNSICVYPMKNSTTYYY
                     GTAGWGIVQASAAWNYDTVYNKTNSTIQNIYADNSPYQFITDIAADSKQNIWCVNKMV
                     QYPLVVKTLNNQWFKFNLSGDIGNNFERLLIDKQNTIWIAGESKLVAFNANGTIENSS
                     DDSYAYIQLSDDEGNIADRTTALAMDNEGVLWIGTTQGIARHTSPYEVFRGKKNISRI
                     KIEIDGEVGYLLSSEKITCIAVDGANRKWIGTQNSGLFVISADGTKQLANYMTTNYPL
                     PSNTINSISIQHSSGEVFIATDKGLVSVMSDATQGSETMEEIKIFPNPVREDYRGNIY
                     IQGTVANAIIKITDMSGDLVFQTIANGGTGVWNGTNLLGERVATGVYLVYISNSDGSQ
                     TKVSKLLFIQ"
     gene            2479..>2819
                     /locus_tag="P5243_05385"
     CDS             2479..>2819
                     /locus_tag="P5243_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRS18914.1"
                     /translation="MTKPTIALAADHAGFHMKELIRKHLLKQGYVVHDFGTHSDEDVD
                     YPDYVHPLGYAIENNEYDVGFVFCGSGNGVNMTVNKHQNVRSGLCWNEEIAQLVKAHN
                     NANICAIPARFV"
BASE COUNT         1004 a          359 c          510 g          946 t
ORIGIN      
        1 tgtaatctta cgttagttcc ctgctttcta taaatatatc tttgttaagt gtaatatttt
       61 atgtaagttt gagagttatt ttatatcaac aattcaatgc gaggaatatt tgttactact
      121 ataggctttt taatgtatgt ttgcatatat gcacaagctc caaatacatg gaaagattgg
      181 tattcctata attcagtttt aagtattgaa tctatagatg gaacactata tgctctttgt
      241 tcaaatggtt tgtttactta taatccatca aaagggcaaa ttactaagat ttcaaaaatt
      301 caagggttgt cagatgttga tcttacaact atgaaatata atgaagaagc aaaaaaatta
      361 gtaattggat atacaaatgg tattatagat atactcagtt ttccgggaca aaaaattact
      421 tcaatcccaa caattgcaaa acggcagttg tatggttcta aagctattcg tgatatagta
      481 tttaaacatg atacggcata tattgctacc gattttggtt tagtaactat gaatttgcaa
      541 aataatgaat ttattcatac tgcagtttta ggaaatcaag gtgaatctgt aggtgtaaat
      601 gccgttacta ttgattatac caatgctatt ttatttgcag caacgcaaaa aggaatattt
      661 agtatagggt tacatcaaaa tatatctgat ggttttttat ggaagcacgt acctattggt
      721 gtacaccagt tttctaaaat tactcatatc ttgtattata ataatgttat gtattacagt
      781 gctgctcaaa ataattggga acaatatgat actatttaca gttatagtaa ttctaataca
      841 agtgtatttg acaaagttat ttatcttgaa aaaattactt cgtattctca aaatctagct
      901 atagtttcac gaaggcaaat aaaactctat aataatgggg aattgctaaa tattataacg
      961 aataccgtac aagaagttaa tttttccgga atatgtaatt gtaataatgc tctctgggta
     1021 agtgattatg caaaaggtat atatagtatt gaaaattata aatcgtatta tccgcagggg
     1081 ccttattcaa atattacttc agaagttttt tgtagaaaca atgcggtaca cttagtatgc
     1141 ggtagttcat cggcgtatca gataggacaa tacaatgttt tagttaatgg tacatggtat
     1201 ggtcatgtta attggaatgt aaaaaattcc atatgtgtat atcctatgaa gaatagtact
     1261 acatattatt atggtactgc aggttgggga atagtacaag catctgcagc atggaactat
     1321 gatacagtat ataataaaac aaacagtaca attcaaaata tatatgccga caattccccg
     1381 tatcagttta taaccgatat agctgctgac agtaaacaaa atatatggtg tgtcaataaa
     1441 atggtgcagt atccattggt agtgaaaact ttaaataatc agtggtttaa atttaatttg
     1501 agcggtgata taggaaacaa ttttgagcga ttgttaatag ataaacaaaa tactatttgg
     1561 atagcaggtg aaagtaaatt agttgccttt aatgcaaatg gaactattga aaattcgtca
     1621 gatgattcat atgcatatat tcaattgtct gatgatgagg gtaatatagc agatagaact
     1681 acggcgttgg ctatggataa tgaaggggtt ttatggatag gaacaacaca aggtatagct
     1741 cgacatacaa gtccgtatga agtgtttagg ggtaaaaaaa atatttcgcg tataaaaatt
     1801 gaaattgatg gtgaagttgg atatttatta agtagtgaaa aaattacttg tatagctgta
     1861 gatggtgcca atagaaaatg gattggtact caaaattcag gcttatttgt aatttcggct
     1921 gatggaacaa aacaacttgc aaattatatg acaacaaatt atcctttgcc ttcaaatact
     1981 attaattcta tatcaataca acattcttca ggtgaagtat ttattgcaac cgacaaaggt
     2041 ttagtttctg ttatgagcga tgctacacaa ggtagcgaaa caatggaaga aattaaaata
     2101 tttccaaatc ctgtacgtga agattatagg ggtaatatat atattcaagg tactgtagca
     2161 aatgcaatta ttaaaataac cgatatgtcc ggtgatttag tgtttcaaac tattgcaaac
     2221 ggtggtacag gagtatggaa tggaacaaat ttacttggag aacgtgttgc taccggcgta
     2281 tatttagtgt atatatcaaa tagtgatggc tcacaaacaa aagtaagtaa actattgttt
     2341 atacaatagt tcacaatatt tagatgcaat aatgattata tagttctata actttttttg
     2401 atagagtaaa atattacagt acatttgcaa aaattaaata agcgttatac gcattagaat
     2461 aacattaata caatacaaat gacaaaacct acaattgctc ttgctgccga tcatgcagga
     2521 tttcacatga aagaacttat tcgtaaacat ttattaaaac aaggatatgt tgttcatgac
     2581 tttggtactc attccgacga agatgtagat tatcctgatt atgttcaccc attaggatat
     2641 gcaatagaaa ataatgaata tgatgttgga tttgtatttt gcggttcggg taatggagta
     2701 aatatgactg taaataaaca tcaaaatgtt cgttcgggtt tgtgttggaa tgaagaaata
     2761 gctcaattgg tgaaggctca taacaatgca aatatttgtg ctattcctgc tcgttttgt
//
LOCUS       DAOTOW010000250         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27863_length_2819_cov_2.964906, whole genome shotgun sequence.
ACCESSION   DAOTOW010000250 DAOTOW010000000
VERSION     DAOTOW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27863_length_2819_cov_2.964906"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="P5243_05390"
     CDS             complement(<1..365)
                     /locus_tag="P5243_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRS18915.1"
                     /translation="MNNTIISIHDKKFTPLISEQEINEKIRVIAHQINNLCLQDIVLV
                     SVLNGAFMFTAELCKHIDSFPIISFIKVASYQGTESTGNITQLIGLQENIEGKTVIII
                     EDIIDTGTTIDTIYNKCKE"
     gene            534..1766
                     /locus_tag="P5243_05395"
     CDS             534..1766
                     /locus_tag="P5243_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS18916.1"
                     /translation="MFKSAKGRAFYLLTLICIIYLLDYADRKVMSGIFESVKAEWLLT
                     DSQLGFLNGIVSLMISVFVIPMSILVDRWSRKNMISLMVFVWSIATLLCAFAQNYYQL
                     LFFRALTGLGEAAYAAAAVAMITKAFPRAHRTKYIGVYDAAAPIGSGIGIMLGGYIAE
                     MYGWRHAFGLVAAPGIIVSLLFLTIRDYHTVQISEKLKESISLSKEVLKDCLYLFKIP
                     TLWFIYFAYSLIIGLNTCVIDWVPTFFQRFHGLSEKEAALKAGILAVAVLIGAPLGGF
                     ISDVWEKRNKHAKILVSAISTAISIVFLFLAIQTTSMVLAVLFFGLFGVHTVAFLAPA
                     SSITQEVVRTNVRALSFGINVLIMNLFAFAIPVLVGKISDIFDLRFALSLLPILGIIA
                     IVLFILVRNQYHIDRNNL"
     gene            complement(1779..2426)
                     /locus_tag="P5243_05400"
     CDS             complement(1779..2426)
                     /locus_tag="P5243_05400"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRS18917.1"
                     /translation="MTDSFLFKGQRQRLVQELQTKGITHSGVLQAIGTVQRHMFFNDS
                     ILQSHAYDDKAFPIDAGQTISQPYTVAFQTQLLNIQSKDKVLEIGTGSGYQAAVLYAL
                     GARVYSIERQKQLYFKTKQLLKKMNIFVETFLGDGYKGLPQFAPFDKIIITAAAPFIP
                     RPLLDQLTIGGIMVVPIGEGIQQKMLKIKKLSDNEFEQSEHGTFSFVPMLGGIAE"
     gene            complement(2416..>2819)
                     /locus_tag="P5243_05405"
     CDS             complement(2416..>2819)
                     /locus_tag="P5243_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18918.1"
                     /translation="SQSHKNNIEAYFHETFHLLHTTVKTYSIDLLIGSSGPFDTFKSI
                     LSPHISKSQSYCTLQFDELQQLHTILTSSTLDEIAAIPGMDQSRIDMLPIASSITLYI
                     CKQLGITSIIQSEYSLKEGVIYTLLQQANDR"
BASE COUNT          831 a          376 c          513 g         1099 t
ORIGIN      
        1 ttttctttac atttattgta tattgtgtct atggtggttc ctgtatcaat tatgtcttca
       61 attattatta ctgttttacc ctcaatgttt tcctgcaatc caattaattg tgtaatattt
      121 cctgtagatt ccgttccttg gtatgaagct acttttataa aacttataat aggaaatgaa
      181 tcaatatgtt tacataattc tgcagtaaac ataaatgctc cgtttaagac cgatactaat
      241 acaatatctt gtaaacaaag attattaatt tggtgtgcaa ttacacgaat tttttcatta
      301 atttcttgtt ccgaaattaa cggtgtaaat tttttgtcgt gtatggaaat aattgtatta
      361 ttcattttag tgtacaaaca tacaaatttt acggaaatgt atatgtaaaa aaacgaatga
      421 tttagtgcaa tataaaatat gttttttata ataatgctta catttactct tattctaaat
      481 attggttagt ttcaaaaaaa atgattttat ttgtaaaata aacaggtgtt attatgttta
      541 aatctgctaa aggaagagcg ttttatttac taactttaat ttgtatcatt tatttattgg
      601 actatgctga ccgtaaagta atgtccggaa tatttgagtc tgttaaagct gaatggttgt
      661 taactgattc gcaacttgga ttccttaatg gtattgtttc gcttatgatt tcggtatttg
      721 taataccaat gtctattctt gttgacagat ggagtcgtaa aaatatgatt tcattaatgg
      781 tgtttgtatg gagtattgct actcttttgt gtgcctttgc tcaaaattat tatcaattat
      841 tattttttag agcacttacc ggtttaggcg aagcagcgta tgcagcagcg gcagtagcaa
      901 tgattaccaa agcattccca cgagctcatc gtaccaaata tattggggtg tatgatgctg
      961 ctgctcctat aggttcagga attggtatta tgttgggggg atatatagcc gaaatgtatg
     1021 ggtggcgaca tgcttttggt ttagtggctg ctcccggaat tatagtatcg ttattatttt
     1081 taactattcg tgattatcat acggtacaaa tttcagaaaa acttaaggag agcatttctt
     1141 tgtcaaaaga ggtgcttaaa gattgtttgt atttgtttaa aataccaact ttatggttta
     1201 tttattttgc gtattcactt attattgggt taaatacttg tgtaatagat tgggtgccta
     1261 cgttttttca acgttttcat ggtttaagtg aaaaagaagc ggctttaaaa gccggtattt
     1321 tagctgtagc agttttaata ggtgctccat tgggtggttt tatttccgat gtatgggaaa
     1381 aaagaaacaa gcacgctaaa atattagttt cggcaatttc tacggcaatt tctattgtat
     1441 ttttgtttct tgctattcaa acaacatcta tggtgcttgc ggtattgttt tttggacttt
     1501 ttggggtaca tactgtagca tttttagcac ctgcatcatc tattacacaa gaggttgttc
     1561 gtaccaatgt aagagcattg tcttttggaa taaatgtgtt aattatgaat ttgtttgcat
     1621 ttgcaattcc tgttcttgta ggaaaaattt ctgatatatt tgatttgcga tttgcattgt
     1681 cgttattacc tattttggga attattgcta ttgttttatt tattcttgtt cgcaatcagt
     1741 atcatattga tagaaacaac ttatagtgtt gtataaattt attcagctat acctcctaac
     1801 attggtacaa aactaaatgt accatgttct gattgttcaa attcattgtc tgatagtttt
     1861 ttaattttaa gcattttttg ttgaatgcct tcaccaatgg gaactaccat aattccccct
     1921 attgtcaatt gatctaataa aggtcttggt ataaaaggag ctgctgcagt tattatgatt
     1981 ttatcaaatg gagcaaattg gggtagacct ttgtagccgt cacctaaaaa agtttcaaca
     2041 aaaatattca ttttttttag caattgcttg gttttgaaat atagttgttt ttgccgttca
     2101 atactataaa ctcttgcccc caaagcatat aatacagcag cttgataccc agaacctgta
     2161 ccaatttcaa gtactttatc ttttgattgt atgttgagta attgtgtttg aaatgccact
     2221 gtgtatggtt gtgaaattgt ttgaccggca tctattggaa acgctttgtc atcgtatgca
     2281 tggctttgga gaatactgtc attaaaaaac atatgacgtt gaacggtgcc aatagcttgt
     2341 aatactccag aatgagtaat accttttgtt tgtagttctt gtactaatcg ttgtcgttgt
     2401 cctttaaata aaaagctatc tgtcatttgc ttgttgtagt aatgtgtaaa taactccttc
     2461 ttttaatgaa tattcagatt gaataataga tgtaatacct aattgtttgc aaatatataa
     2521 tgtaatgcta gaagctatgg ggagcatatc tatgcgagat tgatccatac cgggaatagc
     2581 agctatttca tctaaagtag atgaggtaag tattgtatgt aattgttgta attcatcaaa
     2641 ttgtaaagtg caatatgatt gagattttga aatatgaggt gataatattg atttaaatgt
     2701 atcaaatggt cctgaagatc caataagtaa atcaattgag tatgttttta ctgtagtatg
     2761 aagtaaatga aaggtttcgt gaaagtaagc ttctatgttg tttttgtgtg attgagaaa
//
LOCUS       DAOTOW010000251         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_27958_length_2811_cov_2.959361, whole genome shotgun sequence.
ACCESSION   DAOTOW010000251 DAOTOW010000000
VERSION     DAOTOW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27958_length_2811_cov_2.959361"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="P5243_05410"
     CDS             <1..470
                     /locus_tag="P5243_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18919.1"
                     /translation="TSKLRSFTCVQGGLSYQVGKQLGLPKDIELKCGGKFEQTQRDGA
                     VPIDLQSISADAGISVETIKNLHLLLGFKYMQAQGNEALVVRNRFNEATSGVTKLTID
                     SQEQLLGIGFRYAFANSVFLSTQYILGTYTDNTPNNKNSFDTRQWFVNFNYSF"
     gene            515..1699
                     /locus_tag="P5243_05415"
     CDS             515..1699
                     /locus_tag="P5243_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18920.1"
                     /translation="MIHSKHIYVVTLLSLLLGACATQFSPIDLNTSYMTSIHSGDVVQ
                     GEKIIASGKPVLKHNKGDLLKDRYWWALSGIEIVPQKEGVAFDLTNIGPKYTPFGADL
                     PLLDFKQQPVVLKITARAESKDGSLPLISLQFDDAEGMQANAKRPEARIGNTQEFLDY
                     YFDCSDMWLQTWPDKKNVNATIINKWLFFVNPGGSGYTGRIYIKGIQALPIDSLKPNP
                     EFKIPVGIEGGVVADFKTKDISNWWNSASYTLSWEPDSSLKVVCSNAGPGYAAFGMKL
                     PQTMNAKMAYRAIIKARFEGQGYPELRMDLIDCNGSLSNGNLQSNWLEPTKNKEFVEY
                     LYIYKDDVFQAYPTRRELDKERITDLLLFVNPGKDGWSGIIYIDEIKFIYTGEVGRQS
                     DR"
     gene            1919..>2811
                     /locus_tag="P5243_05420"
     CDS             1919..>2811
                     /locus_tag="P5243_05420"
                     /inference="COORDINATES: protein motif:HMM:NF037325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycan-binding surface protein"
                     /protein_id="HRS18921.1"
                     /translation="MKKYRIYLWTIALTVVLFSCNREQDPIPEPDPTKNKIEMVINDF
                     DGNGLLPGIWSSTNDGAEMTDYKLAVNPKKGLTDTVLYMSGTDENKNWWIGTSLGGDR
                     MGQPFGLPADASKTVIQFEVYATTSNSNLELQLQEADGDVFSWNLGGDGGYQPTAGEW
                     ITYQSAPLSEFNLAGFNNGGQGDGKFAAELLANVSIALISGNSTGKTSSIYINNVVFV
                     VTEVSHVARPTNLKAQSVSKNQINLSWSDNSANEIGFEIERAFGSGAYAVVATVPANT
                     TRYSDMELTANTSYSYRVRAIT"
BASE COUNT          918 a          433 c          598 g          862 t
ORIGIN      
        1 caacatcaaa attacggtcg tttacctgtg tgcaaggtgg attgagttat caagtaggca
       61 aacaattagg attgcccaaa gatatagaac taaaatgtgg aggaaaattt gaacaaacac
      121 agcgcgatgg agctgttcct atagatttac aaagtatatc ggcagatgca ggtatatcgg
      181 tagaaactat taaaaattta catctgttgc ttggatttaa atatatgcag gcacaaggta
      241 acgaagcttt ggttgtaaga aataggttta acgaagctac atcgggtgta actaaactta
      301 ctattgacag tcaagaacaa ttgttgggaa taggctttcg atatgcgttt gcaaattcag
      361 tatttttaag tactcaatat attcttggaa cctataccga caatacacca aacaataaaa
      421 attcgtttga tactcgtcag tggtttgtta attttaatta tagtttctaa tgtattgtga
      481 taatttgaaa cataaacaaa gtaaaaattt tgctatgata cattccaaac atatatatgt
      541 ggttacgcta ctcagcttat tgctgggggc ttgtgcaaca caattcagtc ctattgacct
      601 caatacttcg tatatgacca gtatacattc gggtgatgtg gtgcaaggtg aaaaaataat
      661 agcatcgggt aaaccggtac ttaaacataa caaaggagat ctgctcaaag accgttattg
      721 gtgggcattg tcgggaattg aaatagtgcc gcaaaaagaa ggtgttgcgt ttgatttaac
      781 caatatagga cctaaatata caccttttgg tgccgatttg cccttattgg attttaaaca
      841 acaacccgta gttcttaaaa ttacagcacg tgccgaatct aaagatggta gtctgcctct
      901 tataagtctt cagtttgatg atgccgaagg tatgcaggcc aatgctaaac gccccgaagc
      961 tcgtattggt aatacccaag agtttttaga ctattatttt gattgtagcg atatgtggtt
     1021 gcaaacttgg cccgacaaaa aaaatgtaaa tgctacaatt attaataaat ggttgttttt
     1081 tgtaaatccc ggaggttcgg ggtataccgg acgaatctac ataaaaggta ttcaagcctt
     1141 acctattgat tctttgaaac caaaccctga atttaaaata ccggtgggaa tagaaggcgg
     1201 tgtggttgcc gattttaaaa caaaagatat tagtaattgg tggaatagtg caagttatac
     1261 tctatcttgg gaacccgata gctctcttaa agttgtatgt tctaatgcgg gtccaggtta
     1321 tgctgcattt ggtatgaaat tgcctcaaac catgaacgca aaaatggctt atcgtgctat
     1381 tatcaaagca cgttttgaag gacaaggata tcccgaattg cgaatggatt taatagattg
     1441 taacggtagt ttgtccaatg gcaatctgca atcaaactgg cttgagccta ctaaaaataa
     1501 ggaatttgta gaatatctct atatctataa agatgatgtg tttcaggcgt atcctacacg
     1561 acgagaactt gacaaggagc gtataaccga tttgttattg tttgttaatc ccggaaaaga
     1621 tggatggtcg ggaataatat atattgacga aattaaattt atttataccg gcgaagtcgg
     1681 aagacagtcg gataggtagt tttgagtttt tagttatgag ttcttagtta tgaaatgtta
     1741 atgtgtgaaa atctgtggta aaaaaatgta taggtttact gtataaggta tatgagtcgt
     1801 caattgttta cttttgtgta actccaagtt ctttatggta aataatctgt gttaatctga
     1861 gtaatctgtg gtaaaaaaat aaatatctgt gtaatctgtg gtgaaaaaaa ataaaacaat
     1921 gaaaaaatat agaatatatc tttggactat agcacttaca gtagtgttgt tttcatgtaa
     1981 tcgtgaacaa gatccgatac ctgaacccga tccaactaaa aacaaaattg aaatggtaat
     2041 caatgatttt gacggaaacg gattattgcc cggtatatgg tcgagtacta atgacggagc
     2101 cgaaatgacc gattacaaat tagcagtaaa tcctaaaaaa gggctaacag atacggtttt
     2161 gtatatgtcg ggaaccgatg aaaacaaaaa ttggtggata ggaacatcgc tgggaggtga
     2221 ccgtatggga cagccttttg gtttgcctgc cgatgcctcg aaaacggtga tacagtttga
     2281 agtgtatgca actacctcaa attcaaatct tgaattgcaa ttgcaagaag ccgatggcga
     2341 tgtgttttcg tggaatcttg gtggcgacgg cgggtatcaa cctaccgcag gtgaatggat
     2401 tacgtatcag tctgcaccat taagtgaatt taatttagca ggatttaata atggcggaca
     2461 aggtgatgga aaatttgctg cagaattatt ggcaaatgtg agtattgctc ttatttcagg
     2521 taattctacc ggaaaaactt cttctatcta tataaacaat gttgtatttg tagttaccga
     2581 agtaagtcat gttgctcgtc ctacaaatct taaagctcag agtgtatcta aaaatcaaat
     2641 aaatttatca tggtctgata attcagcgaa tgagataggt tttgaaatag aacgtgcatt
     2701 tggtagcgga gcgtatgctg ttgttgcaac agttcctgct aatactacac gttattccga
     2761 tatggaattg acagcaaata cttcttattc atatagagta cgagctatta c
//
LOCUS       DAOTOW010000252         2794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28167_length_2794_cov_3.752829, whole genome shotgun sequence.
ACCESSION   DAOTOW010000252 DAOTOW010000000
VERSION     DAOTOW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28167_length_2794_cov_3.752829"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="P5243_05425"
     CDS             complement(<1..366)
                     /locus_tag="P5243_05425"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRS18922.1"
                     /translation="MKEGIPTENTTTKAEKQKNQPRNFTSFKSVIAGDVFSSEKVEKH
                     AFYMLFVILLVIFYIGNGYHAYTLEKQNKRLEQEIKELRAEFVSTQAVLIEKMKYANI
                     YKQIQAQGIDLQELKNPPYT"
     gene            complement(363..1259)
                     /gene="rsmH"
                     /locus_tag="P5243_05430"
     CDS             complement(363..1259)
                     /gene="rsmH"
                     /locus_tag="P5243_05430"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRS18923.1"
                     /translation="MNYHVPVLLHESIEGLNIVPQGTYVDATFGGGGHSSLILKNISG
                     GKLFGFDQDEDAKLNIPDNQNFTFVHHNFRYIKRFLRYYNVTQVDGILADLGVSSHHF
                     DTPERGFSFRFNATLDMRMDTTTQLKAHDVVNTYDVDSLTYVLKEYGELPNAKKVAYT
                     IVQEREKQTIHTTEDLKHVCKRFCNPKTESKFLSQVFQALRIEVNKEIEVLQEFLQAG
                     LEVLKPGGRFVVITYHSLEDRLVKNFFKTGNIQGVVKKDFYGNVQSPFQIISKKIITP
                     THEELEQNNRSRSAKLRIAEKK"
     gene            complement(1269..1745)
                     /locus_tag="P5243_05435"
     CDS             complement(1269..1745)
                     /locus_tag="P5243_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRS18924.1"
                     /translation="MITFIGEHSGKLDVKGRVPLPSAFKRQFNKPDDALRFVLKKSNY
                     KECLELHPLDSWQAMMSQLTKKLNPIFNKKHNIFLTQFSKGTVELTLDGVGRLLISKN
                     LLDYASLDKDVVFLGVGNIIELWDKNKYDSNESMLPADEFEQLAEDIFGDNFNLYE"
     gene            2224..>2794
                     /locus_tag="P5243_05440"
     CDS             2224..>2794
                     /locus_tag="P5243_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRS18925.1"
                     /translation="MPLFNSIISWLTVKRLQQINWYKLYPQEIQNEVLFQLIKECEET
                     EFGILHSFHSIQSIRDFQKQVPIHDYESLKPYIDRIREGEQNILWHSPIKWFAKSSGT
                     TNDKSKFIPVSKEALENCHFRGGRDTILLYNHNYPDSELWKGKSLALGGSHQISKISM
                     DSYFGDLSAILIQNMPFWAQFYRTPDLSVA"
BASE COUNT          948 a          510 c          362 g          974 t
ORIGIN      
        1 agtataaggc ggatttttta attcctgcaa atcaattcct tgcgcttgta tttgtttata
       61 aatatttgca tatttcattt tttcaatcaa aactgcttga gtcgatacaa attcggcacg
      121 cagttctttt atttcttgtt cgagtcgttt attctgtttt tctaatgtat aagcatgata
      181 cccattacct atataaaaaa ttaccagaag tatcacaaac aacatataaa aggcatgttt
      241 ctcaaccttt tcactcgaaa aaacatcacc ggcaatcaca cttttaaatg atgtgaaatt
      301 acgtggctga ttcttttgtt tttcagcttt ggtggttgta ttttctgtag gtattccctc
      361 tttcattttt tttcagctat tcgtaatttt gcacttcgtg atctattatt ttgttctaat
      421 tcttcatgag ttggcgttat tatttttttt gaaattattt gaaacggact ctgaacatta
      481 ccataaaaat ctttttttac gactccttga atattgccgg ttttaaaaaa atttttaacc
      541 aatctatctt ccaaagaatg atatgttata acaacaaacc gtcctcctgg ttttaacacc
      601 tccaatcctg cctgtaaaaa ttcttgcaac acctcaatct ctttgtttac ctcaatacgc
      661 aatgcctgaa aaacctgcga taaaaattta gattctgttt tagggttaca aaaacgctta
      721 cacacatgtt ttaaatcctc cgtagtatgt attgtttgct tttctcgctc ttgtactata
      781 gtgtatgcaa cttttttagc atttggcaat tccccatatt ccttcaacac ataagtcaat
      841 gaatctacat catacgtatt cacaacatca tgcgctttca attgagttgt agtatccata
      901 cgcatatcaa gtgttgcatt aaagcgaaat gaaaaacctc gttcaggagt atcaaaatga
      961 tgcgacgaaa cacctaaatc agcaagaatt ccatctactt gagtaacatt ataataacgc
     1021 aaaaagcgtt ttatatacct aaaattatga tgaacaaacg taaaattttg attatcggga
     1081 atatttaatt ttgcatcttc atcttggtca aaaccaaaga gtttaccacc agaaatattt
     1141 ttgagaataa gggaggaatg cccaccgcca ccaaacgtag cgtcaacgta tgtcccctgc
     1201 ggaactatgt tgagaccctc tatactttca tgcaagagaa ccggcacatg ataattcata
     1261 tttttcctct attcatacaa attaaagtta tcaccaaata tatcttcagc aagttgctca
     1321 aactcatcag ccggcaacat gctctcattt gaatcatatt tattcttatc ccacaattct
     1381 ataatattac caacacctaa aaaaaccaca tctttatcaa gcgaggcata atcaagaaga
     1441 tttttagaaa tcaacaatct acctacgcca tcaagagtaa gctctacagt tcctttacta
     1501 aattgagtta aaaaaatatt atgcttttta ttaaatatag gattcagttt ttttgtcaat
     1561 tgcgacatca ttgcttgcca tgaatcaagc ggatgcaatt ctaaacattc tttataatta
     1621 ctttttttaa gcacaaaacg caaagcatca tcaggcttat taaactgcct tttaaatgcc
     1681 gatggtaaag gaactcttcc ttttacatct aattttccgc tatgttctcc tatgaaagta
     1741 atcatctaaa aaaatcgcct atactaaaat ttttgtaaaa atatatcata atttcccatt
     1801 ttcttacatt tttttccact ttttaccact ttgttaataa cttttatatt tttaccatat
     1861 cttttcataa aaaatgagaa ttttatgtat taacagtatt taccaaacaa cactgttgaa
     1921 ggaattaaaa aaatgaataa gaattagata ttttcaagtg actttatact attaaaaatc
     1981 aatatatcaa ttaattacac aaaaaaacat ataataaaaa gaatataaac cgaattattc
     2041 ttaaaacttt taatcaaacc atatttatct aaatagaatt ttaagaatta aaaaattaca
     2101 cgctaaaaat tcgaagcaca ggctttattc aaaatttatc gttttcgtat tttttgaaac
     2161 ttctcttgat atattcacgg caaatgccta tttttgtagg gtaaatttat aatcacagtt
     2221 tgcatgcctt tatttaattc tattatttct tggttaacag taaagcgatt acaacaaatt
     2281 aattggtata aactctatcc gcaagaaatt caaaatgaag tactttttca attaatcaaa
     2341 gaatgtgaag aaacagaatt cggaatttta cactcgtttc attctattca aagtattcgt
     2401 gattttcaaa aacaagtacc tatacacgat tacgaatcgc tgaaacctta tattgacaga
     2461 atacgcgaag gagaacaaaa tatattatgg cactcgccta tcaaatggtt tgcaaaaagt
     2521 agtggcacaa ccaacgacaa aagtaaattt ataccggtaa gtaaagaagc tttagaaaac
     2581 tgccactttc gaggtggtcg cgatactatt ttattataca atcacaatta tcctgattct
     2641 gaactatgga aaggtaaaag tttggcgtta ggtggtagtc accaaatcag caaaatttca
     2701 atggattcat attttggtga cttatctgca attcttattc aaaatatgcc attttgggcg
     2761 caattttatc gcacccctga tttatcggta gcac
//
LOCUS       DAOTOW010000253         2779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28357_length_2779_cov_2.935389, whole genome shotgun sequence.
ACCESSION   DAOTOW010000253 DAOTOW010000000
VERSION     DAOTOW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28357_length_2779_cov_2.935389"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="P5243_05445"
     CDS             complement(<1..481)
                     /locus_tag="P5243_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18926.1"
                     /translation="MKRIAFWLAVVGFISVYSQVTAQSESLNYGSGKDSIACVENLAI
                     YQHRYKNEEKTGTFSPETINAWRKAFNSCPQSSKNMYVPHGVNMYTTLYRNEKDAVKK
                     QKYLDTILLIYDRRIQNFGEENKFIGLKGEDLMLLNNAKYKEAFNMCKKSVDALGNKS
                     "
     gene            complement(509..1828)
                     /locus_tag="P5243_05450"
     CDS             complement(509..1828)
                     /locus_tag="P5243_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18927.1"
                     /translation="MRINKKIIVLLLSVASIQAFAQRSTTSLYSRYGYGELYIPSCNF
                     VQMGNVANGIRSAKQIQFINPAHNTAIEKETFLFQVGSGYSYRNFAQQNQHVSKIDAG
                     IEYMAMAFAVIPKKWGMAAGIVPFNTVGYEIQAADSLAQYTYSGKGGINQFIWSNSIS
                     PVKGLSIGANASLLFGKTIYTSNAKVLVDEFSYYTRKQQIYDAKGFMWDAGLQYTYPI
                     NDKSSVTFGATYRNKQTFSFHKQSYLGTYNVLEQIDVNKKKRKTITTVYESYTEIDTL
                     HNIITRNIESDIPQQVSFGIGYSIQDKFTGAFDCGYSNWNSVSVWGKPSIYTGNAKFA
                     RLGVEFIPDKRGASYFKKIPYRIGLQYNELPISYTFNNTLLKPIDFGISFGSEFLLKQ
                     TANSLAVSVITGRRGDISSAQSLQELYTMIKLQINLKETWFLQRKID"
     gene            complement(1815..2540)
                     /locus_tag="P5243_05455"
     CDS             complement(1815..2540)
                     /locus_tag="P5243_05455"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRS18928.1"
                     /translation="MYLVIDCGNTSIKTYYFENSHLQNSQRYALSEVSKLLHDIGQNK
                     AITQAIFCSVSYNTSLLKQIQSIVPCIEFNHTTKIPLINNYSSPQTLGPDRLAAAIGA
                     ETLAPACNKLIFDFGTAITIDFVSEDGIYHGGNISPGLHTRCKSLQQNTQLLPLITDF
                     SNHSLTGKSTLEAIKNGVVLGIIYEIEGYIAAYNTIYENISLFFTGGDAFFFEKTVKK
                     TIFAEPNLIAFGLHRILQDNADK"
BASE COUNT          859 a          431 c          439 g         1050 t
ORIGIN      
        1 cggatttgtt tcccaatgca tcaaccgatt ttttacacat attaaaagct tctttatatt
       61 tagcattatt taaaagcatt aaatcttctc cttttaatcc aataaattta ttttcttcac
      121 caaaattttg gatacgtctg tcataaatta ataaaatagt atcaagatat ttttgttttt
      181 ttacggcatc tttttcattt ctgtacaaag ttgtgtacat atttactccg tgaggaacat
      241 acatgttttt tgaagattgc ggacatgagt tgaatgcttt tctccatgcg ttgatagttt
      301 caggtgaaaa tgttcctgtt ttttcttcat ttttgtatcg gtgttgatat attgctaaat
      361 tttcaacaca tgcaatacta tcttttcctg aaccataatt taatgattca ctttgcgcag
      421 ttacttgtga atatacactt ataaatccta ctactgccaa ccaaaatgct attcgtttca
      481 tatttgtatt gttttatata tcagtttgtt aatcaatttt cctttgtaaa aaccacgttt
      541 cttttaagtt tatttggagt tttatcatag tatacaattc ttgtaatgat tgtgctgatg
      601 atatatctcc acgtcttcct gttataacac ttacagccaa tgaatttgct gtttgtttta
      661 acaaaaattc agagccaaaa cttatgccaa aatctattgg cttaagtaat gtattgttaa
      721 atgtatacga tataggtaat tcattatatt gtaaaccaat tctatatggt atttttttga
      781 aatacgaagc acctcgttta tcgggaataa actcaactcc caatcgcgca aattttgcat
      841 taccggtata aattgacggt ttaccccata ccgaaacaga attccaattt gaatagccac
      901 aatcaaaagc tccggtaaat ttatcttgta tagagtaacc tattccaaaa cttacttgtt
      961 gtggaatgtc agattctata tttctagtta ttatattatg taatgtgtca atttcggtat
     1021 atgattcata cacagtagtt atggtttttc gtttcttttt gtttacatct atttgttcta
     1081 aaacattata cgttcctaaa taagattgtt tatgaaacga aaatgtttgt ttgtttctgt
     1141 aagttgcacc aaatgtaaca gaagatttat cgttgatagg atacgtatat tgaagaccgg
     1201 catcccacat aaaacctttg gcgtcatata tttgttgttt acgtgtgtaa tacgaaaatt
     1261 catcaaccaa tactttagca ttggaggtat aaatggtttt tccaaataac aatgatgcat
     1321 tagcgccaat tgatagtcct ttgaccgggc ttatggaatt actccaaata aattgattga
     1381 taccgccttt acctgaatat gtatattggg caagtgaatc ggcagcttga atttcgtatc
     1441 ctactgtatt aaatggaact atacctgcag ccatacccca ttttttcggt attacggcaa
     1501 aagccattgc catatattca ataccggcat caattttaga tacgtgttgg ttttgttgtg
     1561 caaaatttcg atacgaatag ccgcttccta cttggaagag aaatgtttct ttttcaattg
     1621 ctgtgttgtg agcaggattt ataaattgaa tttgcttggc cgaacgaatt ccgttagcaa
     1681 cattgcccat ttgcacaaag ttacacgaag gtatatacaa ctcaccatat ccgtatctgc
     1741 tgtataatga tgtagttgag cgttgtgcaa aagcttgaat tgaggcaact gacaacaata
     1801 gtacaattat ttttttattt atccgcatta tcttgtaata ttctatgtaa tccaaaagca
     1861 attaaatttg gttctgcaaa gatggttttt tttactgttt tttcaaaaaa aaatgcatcg
     1921 ccaccggtaa aaaacaacga aatattttca taaatggtat tgtaggctgc aatatatcct
     1981 tcaatttcat atataattcc taaaacaaca ccatttttta tggcttccaa agttgatttt
     2041 ccagtaagtg aatgatttga aaaatcggta attaaaggaa gtaattgggt gttttgttgt
     2101 aatgatttac aacgtgtatg gagtccgggt gatatatttc caccatggta tatgccatct
     2161 tctgaaacaa aatctatggt aatagctgta ccaaaatcaa aaattaattt attacacgca
     2221 ggtgctaatg tttctgctcc aatagctgcc gcaagtctat cagggcctaa tgtttgaggg
     2281 cttgaataat tattgatgag tggaattttt gtagtatggt tgaattcaat acaaggcact
     2341 attgattgta tctgtttaag aagagaggta ttgtatgata cactgcaaaa tatagcttgt
     2401 gtaatagctt tattttgacc tatatcatgt aataatttcg aaacttcgga tagagcgtat
     2461 ctttgagaat tttgtaaatg tgaattctca aaataatatg ttttaataga agtatttccg
     2521 caatctataa caagatacat gtaatttttt ttgcaatatt actacttatt ttttagttat
     2581 ttaatgtgta aaaaataatt atagtatatt tgttttcaat aattacatgc atgaatacaa
     2641 ttcgacagat aattcttttt ttatgtattg tttctgtttc tctttttgct caacaagtaa
     2701 aagttgaaca aagttatgtg ttgcccggaa ttattgttga cggtgatacg ttagctctcg
     2761 taaaattacc aaaagtgta
//
LOCUS       DAOTOW010000254         2775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28406_length_2775_cov_2.864706, whole genome shotgun sequence.
ACCESSION   DAOTOW010000254 DAOTOW010000000
VERSION     DAOTOW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28406_length_2775_cov_2.864706"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..2264)
                     /locus_tag="P5243_05460"
     CDS             complement(42..2264)
                     /locus_tag="P5243_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRS18929.1"
                     /translation="MTEFSQEEKDNIEKQFNEILACCPKCKKNDETIALVRKAFDIAN
                     KAHYGSRRKSGEPYILHPIEVAKIVSGEIGLGSKAVVCALLHDVVEDTDLSLEDIESM
                     FDPKIAQIIDGLTKIRGVLKTKAGQAENFKKMIFTLAEDVRVILIKIADRLHNMRTLD
                     SMPLEKQLRISEETQNLFVPLAHRLGFYNIKMELEDLCLKYQNPKIYNEISKKLKGSE
                     KKRVHYLNRFCIPIMMTLDKNGYKFNITSRSKSISSIWRKIQTKGIEFDNVYDVFAVR
                     IILLDVPREEEKSKCWSVYSIITDLYTPNPKRLRDWISTPKDNGYEALHTTVMGPDGN
                     WVEVQIRTERMDEIAERGYAAHWKYKDIDGFEKSQLEVWMERVRSSLSNPDVDALSFL
                     DEFKLNLYATDLFAFTPKGEMKRLPTNATVLDFAFEIHTQIGYHAIGAKINRQKTVAL
                     DYKIQSGDIIEILTTESQKPQKEWLNFITTAKAKNALQQVFKFERKKDILRGQKILHD
                     TLKNNNIQENTTTIDKLLYGYGLIDKEELYEKIGRNEIDLSKIIEYATRKRSSKIVKF
                     WKLQLRLSKSDDDILDEDIDPNIKVLHNTTNYSIADCCRPIPGDPVLAFNVKNDYVIH
                     NEKCPIAIELQTHEKAYKIKWISKNENAFLVSIQIVGDDRIGILNEITRVVSDQLNVN
                     IRTVHIDTSENKFEGTLDLYISNLEHLNNLMMRLQKIKGMQKVIRIELKQDAITYKKH
                     "
BASE COUNT          801 a          436 c          430 g         1108 t
ORIGIN      
        1 aagtaggaaa tacaagctgt aaaatagtaa agtagcaaat actaatgctt tttataggta
       61 atagcatctt gttttaattc tattcgaatt actttttgca tacctttaat tttttgtaga
      121 cgcatcataa gattgtttaa gtgctctaaa ttacttatgt ataaatcaag agtaccttca
      181 aatttatttt cacttgtgtc tatatgaact gttcttatat ttacattgag ttggtccgaa
      241 acaactcgtg taatttcatt taatatacca attctatcgt caccgactat ttgaatactt
      301 actaaaaagg cgttttcgtt tttgctaatc cattttattt tatatgcttt ttcatgtgtt
      361 tgtaattcta tggcaatagg gcatttttca ttatgaatta cataatcgtt ttttacatta
      421 aaagccaata cagggtcacc gggtattgga cgacaacagt cggcaataga atagtttgtt
      481 gtgttgtgaa gtacttttat attagggtca atatcttcat ctagtatatc atcgtcagat
      541 tttgataatc gaagttgtaa tttccaaaat tttacgattt tacttgatcg ttttcgagta
      601 gcatattcta taatttttga taaatctatt tcattacgac caattttttc gtataattct
      661 tctttatcaa ttaaaccgta tccatacagt aatttgtcaa tggttgttgt attttcttga
      721 atattgttgt ttttgagtgt gtcgtgaaga attttctgac cacgtaaaat atctttttta
      781 cgttcaaatt taaaaacctg ttgaagagca tttttggctt tagctgtagt tataaaattg
      841 agccattctt tttgcggttt ttgtgattcg gtagttaaaa tttcaattat atcaccactt
      901 tggattttat aatcaagcgc tacggttttt tgtctattaa ttttagcacc aatagcatga
      961 taccctattt gtgtgtgaat ttcaaatgca aaatcaagaa cagttgcatt tgttggaagt
     1021 cgtttcattt cgccttttgg ggtaaaggca aataaatcgg tagcatatag gttcaatttg
     1081 aattcatcaa gaaaagagag agcatccaca tcggggtttg acaatgaaga acgaacacgt
     1141 tccatccaca cttcaagttg gcttttttca aaaccatcaa tgtctttata tttccagtgt
     1201 gcagcatatc cacgttctgc aatttcgtcc atacgttcgg tacgaatttg tacttcaacc
     1261 caattaccat ccggtcccat tacagttgtg tgtaatgctt cgtaaccatt atccttgggg
     1321 gtacttatcc agtcgcgaag tcgtttaggg tttggggtat ataggtcggt aataattgaa
     1381 tacacactcc aacattttga tttttcttct tcgcgtggta catcaagtaa aataattctt
     1441 acggcaaata catcatatac attgtcaaat tctatccctt tggtttgaat tttacgccaa
     1501 attgatgaaa ttgattttga acgactggta atattaaatt tatatccgtt tttatcaaga
     1561 gtcatcatta tgggtataca aaatctatta agataatgaa cccgtttttt ttcgcttcct
     1621 tttaattttt ttgaaatttc attataaatc ttaggatttt ggtattttaa acataaatcc
     1681 tctaattcca tttttatatt atagaaacca agtctatgag ctaacggaac aaataaattt
     1741 tgtgtttctt cagaaatacg caattgtttt tcaagtggca tagaatcaag ggttcgcata
     1801 ttgtgcaggc ggtctgcgat tttgattaaa ataactcgca catcttcggc gagggtaaaa
     1861 atcatttttt taaaattttc agcttgccct gcttttgttt ttaatacacc tcgaattttt
     1921 gtgagcccat caattatttg tgcaattttg gggtcaaaca tagattcaat gtcttccagt
     1981 gataaatcgg tatcttcaac aacatcatgg agtaatgcac atactaccgc ttttgagcct
     2041 aatccaatct cgcctgaaac tatttttgca acttcaatgg ggtgtaatat atatggttca
     2101 cctgattttc gtctgcttcc atagtgtgct ttgtttgcta tatcaaatgc tttacgaacc
     2161 agggctatgg tttcatcatt ttttttgcat tttggacaac atgccaaaat ctcattaaat
     2221 tgcttttcaa tattatcttt ttcttcctgc gaaaactctg tcattctacc atatgtatta
     2281 tacggttaca aatgtaataa aatgaaacac taaaacaaat taactgttat aacatttttt
     2341 tgaagattga aattattaat tgatagaatt gaatagtatt attttttttg ttttgtaatg
     2401 gttgttaata actagttaat tatgtttttt acttcaacta ttgtaattcc aatttattca
     2461 ttatatattt gcattactgt tacgctttat tattattcct ttaatgaatg gcgcggacaa
     2521 gggaacttcc acctctcttt ccgccctttt tttttgagta tttttactat ctttttgtag
     2581 gtttttgtgt tataatgttg tatatttata ttttaattgc aaccctggag ctatgaatat
     2641 ttttacttta ccattacaaa ttactataaa tggaaattca attcggtatt atcgtggggg
     2701 tagtggggat tcaattattt ttattcatgg tattacaaca tattctttta tttggcgaca
     2761 aatagtgcct tattt
//
LOCUS       DAOTOW010000255         2775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28408_length_2775_cov_2.373162, whole genome shotgun sequence.
ACCESSION   DAOTOW010000255 DAOTOW010000000
VERSION     DAOTOW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28408_length_2775_cov_2.373162"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1154
                     /gene="argF"
                     /locus_tag="P5243_05465"
     CDS             156..1154
                     /gene="argF"
                     /locus_tag="P5243_05465"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRS18930.1"
                     /translation="MSLQLKGKHFLKLLDFSQSEIRGLLDLAHQLKKDKKNGVEKQLL
                     TGKNIALIFEKTSTRTRCAFEVAAYDQGAHTTYLGPSGSQIGHKESMKDTARVLGRMY
                     DAIEYRGYGQHLVEELAKYAGVPVWNGLTDEFHPTQILADFMTMEEHCSKPLNTISFC
                     YMGDARNNMANSLMVGAAKMGMDIRLAAPKSVQPDEQLVSTCKEIAKETGAKILITES
                     VDEAVLGVDFLYTDVWVSMGEPAEVWKERVRLLLPYQINSHVLQKTGNPQVKVLHCLP
                     AFHNRETKVGEEMFQKFGLEAMEITEDVFESEASIVFDEAENRLHSIKAVMVATLA"
     gene            1164..2168
                     /gene="ftcD"
                     /locus_tag="P5243_05470"
     CDS             1164..2168
                     /gene="ftcD"
                     /locus_tag="P5243_05470"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRS18931.1"
                     /translation="MELIECVPNISEGCNSELIEQIAKVISSVSGVKLLHIDAGKAAN
                     RTVYTFVGFPESVVEAAFNMFEFVIKHIDMAQHTGTHPRIGAVDVCPLIPIQGISLEQ
                     TKQYAEQLAKRVGEELHVPVYLYEQNAKYIHRTRLEQIRSGEYEGFSQKMQSEGWDPD
                     YGPHVFNPKTGASVIGARNFLLAYNINLVSSDVTIAKHIAARIRESGGNYTDMFGKKH
                     VSKGLLRGVKAIGWYIPEYDVVQVSTNITDFNLVSMYKVYCEVQKLAIQYGTNVRGSE
                     LIGLVPYDALHNAGKEILQQKPSVANDTIQAAIQELGLQDVKEFTPQKQILEYVAGVL
                     "
     gene            complement(2193..>2775)
                     /locus_tag="P5243_05475"
     CDS             complement(2193..>2775)
                     /locus_tag="P5243_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18932.1"
                     /translation="EMNYGENVTIRIANVITPNNFKLCDSMVYLPQYNSYVEPSVKYT
                     YMKSVCTKDERRDYRIQGNDSICPCVVNNDNIYNGNKNAFLYIEGIDKFPYNKVIIYK
                     EGSTKPLITYNNYQNSLLTFNAYIADTLQDGKTVVFKHLSGNFTYIIKLYNNLLHTDL
                     YDELSGGFTVIRKPQDCPTTGCKGRDADDPLLD"
BASE COUNT          936 a          370 c          528 g          941 t
ORIGIN      
        1 aaatgaggaa atgatttcat aggtataggt ttttatagtt tatatattgc taaaatagaa
       61 aaaataaatt aatgagttat atattttact gtaaattcat ttgtttttaa tcaaaatatg
      121 taagtttgta tataaaaatt taattacgag taattatgtc attacagctt aaaggaaaac
      181 attttttgaa attattagat tttagtcaat cggaaattcg tggattatta gatttggctc
      241 atcagttaaa aaaagataag aaaaatggtg ttgaaaagca attgcttaca ggtaaaaata
      301 tagcattaat ttttgaaaaa acgagtacac gcactcgctg tgcttttgaa gtagcagcgt
      361 atgatcaagg agctcataca acatatttag gtccaagtgg ttcacaaata gggcataaag
      421 aatcaatgaa agatactgct cgagttttgg gtagaatgta tgatgctata gaatatagag
      481 gatatgggca acatttggtt gaagagttgg caaaatatgc cggtgttccg gtatggaatg
      541 gtttgacaga tgagtttcat cctacgcaaa ttttagcaga ttttatgaca atggaagagc
      601 attgttctaa acctttgaat accatttcgt tttgttatat gggtgatgct cgaaataata
      661 tggcaaattc acttatggta ggtgctgcta aaatgggtat ggatattaga cttgctgcac
      721 ctaaatctgt tcagcctgat gaacaattag tttctacatg taaagaaata gcaaaagaaa
      781 ccggagcaaa aattcttata accgaatcgg tagatgaagc tgtattaggg gttgattttt
      841 tatataccga tgtatgggta agtatgggtg aaccggctga ggtttggaaa gaacgtgtac
      901 gtttattgtt accttatcaa ataaattcgc atgttttaca aaaaacgggt aatccacagg
      961 ttaaagtatt acattgtttg cctgcatttc ataatagaga aacaaaagta ggtgaagaaa
     1021 tgtttcagaa atttggatta gaggcaatgg aaattaccga agatgttttt gaatcagaag
     1081 cttctattgt atttgatgaa gcagaaaaca gacttcactc tataaaagct gtaatggtag
     1141 ctactttagc ataatacgtt ggtatggaat taattgaatg tgttccaaat attagtgaag
     1201 gttgtaattc tgaattaata gaacaaatag cgaaagttat ttcatctgtt tccggagtta
     1261 aattacttca tatagatgcc ggaaaagcag caaatagaac tgtatatacg tttgttggtt
     1321 ttcctgaatc cgttgttgag gcagcattta atatgtttga atttgttatt aaacatattg
     1381 atatggctca acataccggt acacatcctc gcataggtgc tgttgatgtg tgtccgctta
     1441 ttccaattca aggaatttca ttggaacaaa caaaacaata tgccgaacaa cttgcaaaac
     1501 gagtaggaga ggaattacat gttccggtgt atttgtatga acagaatgca aaatatatac
     1561 atagaactcg tttagaacaa attcgttctg gtgaatatga aggtttttct caaaaaatgc
     1621 aatcagaagg ttgggacccg gattatggcc ctcatgtttt taatcctaaa acaggtgcca
     1681 gcgtaatagg tgctcgtaat tttttgttag cctataatat aaatttagtt agtagtgatg
     1741 ttactatagc aaaacatatt gctgcacgta ttagagaatc cggtggtaac tatactgata
     1801 tgttcggaaa aaaacatgtg agtaaaggat tgctgagagg tgtaaaagct attggttggt
     1861 atattcccga atatgatgtt gttcaggttt ctaccaatat taccgatttt aatttggttt
     1921 ctatgtacaa agtctattgc gaagttcaaa agcttgcaat tcaatatggt acaaatgttc
     1981 gtggttctga attaataggt ttagttcctt atgatgcatt gcataacgcc gggaaagaaa
     2041 tattacaaca aaaaccttcg gttgcaaacg atactataca agcagcaata caagaactag
     2101 gcttacaaga tgtaaaagaa tttactcctc aaaaacaaat tttggaatat gttgcaggag
     2161 tattataatt tgtattttac gattgtattt atttaatcaa gtaacgggtc gtcagcatct
     2221 cttcctttgc acccggtagt tggacaatct tgaggttttc gtattacggt aaatccaccc
     2281 gataattcat catataaatc tgtatgtaat aagttattgt ataatttaat tatataggtg
     2341 aaatttccac tcaaatgttt aaaaacaaca gttttaccat cttgtaatgt gtcggctatg
     2401 tatgcattaa atgttagtaa actattttga taattattat atgttataag aggtttagtt
     2461 gaaccttctt tatatatgat aactttgtta tatggaaatt tatcaattcc ttctatgtag
     2521 agaaaagcat ttttattgcc attgtaaata ttatcattat ttacaacaca tggacatata
     2581 gagtcattgc cttgaattct gtagtcacga cgctcgtctt tagtgcaaac ggatttcata
     2641 taagtgtatt taacacttgg ttcaacgtaa ctattgtatt gtggtaaata aaccatacta
     2701 tcacataatt taaaattatt aggtgttatt acattggcta tgcgtatagt tacattttca
     2761 ccataattca tttcc
//
LOCUS       DAOTOW010000256         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28591_length_2761_cov_3.019956, whole genome shotgun sequence.
ACCESSION   DAOTOW010000256 DAOTOW010000000
VERSION     DAOTOW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28591_length_2761_cov_3.019956"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5243_05480"
     CDS             complement(<1..605)
                     /locus_tag="P5243_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18933.1"
                     /translation="MISNTKIFAVTGKPILHSKSPNMFNAMFEFANIDAAYVRLAAKD
                     AEQAIFMFKSLGIQGMNVTAPFKEKIIKYLDIIHDEATILKSVNTIIFKKGKLHGYNT
                     DYYGVTQSFVDAGIDIQNKSCLVIGAGGAGKAAAYGMHIKGAKVTITNRTPSKAAKAA
                     KIIGCKHASIDTIEQLVASHEIIISTLHQNINLINEQWLTK"
     gene            758..1216
                     /locus_tag="P5243_05485"
     CDS             758..1216
                     /locus_tag="P5243_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRS18934.1"
                     /translation="MSFQIDSIDKKILNLLMENARMPFLEIARLCGVSGAAIHQRVNK
                     LEEAGLFKGSKYIVEERALGYNTCAYIGVHFEKGSVYSSVVEAIKEIPEVVECHYTTG
                     TYALFLKIYAKDNHHLMEILNHQIQQIPGISSTETFISLEQSIQRQVIVR"
     gene            1224..2411
                     /gene="hflX"
                     /locus_tag="P5243_05490"
     CDS             1224..2411
                     /gene="hflX"
                     /locus_tag="P5243_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRS18935.1"
                     /translation="MGGYVTHDEAERAVLVGVITSDQTEMQAREYLDELAFLAKTAGV
                     EVVTTFTQKLPQTIAKTFIGSGKIAEIKECIDAEGIKLVIFDDELSPSQLRNLEKELS
                     CKILDRTNLILDIFAQRAQTAHAKVQVELAQYQYLLPRLTRMWTHLERQQGGIGMRGP
                     GETEIETDRRVIRDKITLLKKQLEKIDRQMATQRQNRGNMIRVALVGYTNVGKSTIMN
                     MISKSDVFAENKLFATLDTTVRKVVIDNLPFLLSDTVGFIRKLPTHLVESFKSTLDEV
                     READILLHVVDISHKNFQEHIEVVRQTISDIGAGDKTTFLIFNKIDAFTYTPKDEDDL
                     MPKEKSEYSLDDFKQMYFARKETCVFISAKHKQNIEEFKQLLYDKVKEMHAIRYPYNN
                     FLY"
     gene            complement(2416..>2761)
                     /locus_tag="P5243_05495"
     CDS             complement(2416..>2761)
                     /locus_tag="P5243_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRS18936.1"
                     /translation="PYRTTNRAPWPLDCSGYVSMLYGNFGVSLPHSSGAIANVTQTIS
                     IKEAKPGDLIFFKGRDARKNRVGHVALIIEVHDNYIKMMHSTVQKGIIIENYPDSYYY
                     TKRFVKVGRVIL"
BASE COUNT          972 a          399 c          468 g          922 t
ORIGIN      
        1 ttcttagtaa gccattgttc attgataagg tttatatttt gatgtagagt agaaataata
       61 atttcatgag aagcaactaa ttgttcaatt gtatcaatag atgcatgttt gcaccctatt
      121 attttagctg ctttagctgc tttagacgga gttcggtttg taatagttac ttttgctcct
      181 tttatatgca ttccatatgc cgctgcttta cctgctccgc ctgcaccaat aactaaacaa
      241 cttttatttt gtatatcaat acctgcatca acaaaacttt gggtaacacc atagtaatcg
      301 gtattatacc catgaagttt ccctttttta aaaattatgg tatttacact ttttaaaatt
      361 gtagcttcat cgtgaataat gtcaagatat ttaatgattt tttccttgaa aggtgcagta
      421 acattcattc cttgtattcc aagagattta aacataaaaa tagcttgttc tgcatcttta
      481 gctgcaagtc ttacatatgc agcatcaata ttggcaaatt caaacatagc attgaacatg
      541 tttggacttt tactatgtaa aataggtttt cccgtaactg caaatatttt tgtattggaa
      601 atcataacat tgtatttatg acaaaaatag ataaaaaaat attgtaattg ttatgaaatg
      661 tatggtaatc tatttttgta catttgaaac tcataaacaa atttttaaaa atcatttcat
      721 ttttgtatgt ttgtatttac tattaaaaca tacgtatatg tcatttcaaa tagattcaat
      781 agataaaaag attttaaatc ttttgatgga gaatgctcgc atgccatttt tagagattgc
      841 tcggttatgt ggtgtttcag gagcagcaat tcatcaacgt gtaaataaat tagaagaggc
      901 tggtttattt aaaggctcaa aatatattgt tgaagaacga gcattaggat acaatacatg
      961 tgcatatatt ggtgttcatt ttgaaaaagg gagtgtatat agtagtgttg ttgaagcaat
     1021 taaagaaatt ccggaagttg tagaatgtca ttatacaacc ggaacgtatg cattattttt
     1081 aaaaatatat gctaaagaca atcatcattt gatggaaatt ttaaatcacc aaattcaaca
     1141 aatccccgga atttcaagta ccgaaacatt tatttcgctt gaacaatcaa ttcagcgtca
     1201 agtaatagta agataaataa tttatgggcg gatatgtaac gcatgatgaa gcagaaagag
     1261 ctgtattggt aggtgttata acgtcagatc aaactgaaat gcaagctcga gaatatttag
     1321 acgaacttgc atttttagca aaaactgccg gagttgaagt agttacaaca tttactcaaa
     1381 aactgccaca aactatagca aaaacattta taggttccgg aaaaatagcc gaaatcaaag
     1441 aatgtattga tgcagaagga attaaattag taatttttga tgatgagtta agtccttcac
     1501 aattacgaaa tttagaaaag gagttatcat gcaaaatctt agatagaacg aatttaattc
     1561 ttgatatttt tgcacaacgt gctcaaactg cgcatgcaaa agtacaagta gaattagctc
     1621 aatatcaata tttattaccg cgtcttactc gaatgtggac tcacttagaa cgtcaacaag
     1681 gtggtattgg aatgcgtggt ccgggtgaaa cagaaataga aacggaccgc cgagtaatca
     1741 gagacaaaat aacgttgtta aaaaaacaac ttgagaaaat agatcgtcaa atggccacac
     1801 aaaggcaaaa tcgtggcaat atgatacgag tagcattagt aggatataca aacgttggaa
     1861 aatcaactat catgaatatg ataagtaaat ctgatgtgtt tgccgaaaat aaattgtttg
     1921 ctactcttga tactactgta agaaaggttg taattgataa tttaccgttt ttactttctg
     1981 atacagttgg ttttattaga aagttgccta ctcatttggt agaatcattt aaatcaactc
     2041 ttgatgaagt aagagaagcc gatattttgc ttcatgttgt agatatttca cataaaaatt
     2101 ttcaagagca tattgaagta gttcgtcaaa caatttctga tattggagca ggtgataaaa
     2161 caacctttct tatttttaat aaaatagatg cgtttacgta tactcctaaa gacgaagatg
     2221 acttaatgcc taaagagaaa agtgaatatt ctttagatga ttttaaacaa atgtattttg
     2281 ctcgaaaaga aacgtgtgta tttatatcgg ctaagcacaa acaaaatata gaagaattta
     2341 agcagttact gtatgataaa gtaaaagaaa tgcatgctat acgatatccg tataataatt
     2401 ttttgtattg atagattata aaataactct acccacttta acaaatcttt tggtataata
     2461 atatgaatca ggataatttt ctataatgat gcctttttga acagtactat gcatcatttt
     2521 aatgtaatta tcgtgtactt caataatgag agctacgtgt ccaactctgt tttttcgagc
     2581 atctctacct ttgaaaaaaa ttaaatctcc tggtttagct tcttttattg atatagtttg
     2641 tgtaacgtta gcaatagctc cgctggaatg tggcaaagaa acgccaaaat taccataaag
     2701 cattgacaca tagccactac aatctagcgg ccatggtgct ctattagtag tacgatatgg
     2761 t
//
LOCUS       DAOTOW010000257         2760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28606_length_2760_cov_3.402218, whole genome shotgun sequence.
ACCESSION   DAOTOW010000257 DAOTOW010000000
VERSION     DAOTOW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28606_length_2760_cov_3.402218"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..498)
                     /locus_tag="P5243_05500"
     tRNA            complement(415..498)
                     /locus_tag="P5243_05500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(462..464),aa:Leu,seq:caa)
     gene            621..1448
                     /locus_tag="P5243_05505"
     CDS             621..1448
                     /locus_tag="P5243_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRS18937.1"
                     /translation="MIRSIYVVGLFFIVLFYNSYSQNTSPYSLTLKHESVYAAYTISG
                     LSVIYLLNNKLNTHTEEEISNLNAQDIWKIDRNTVTKYSDKTALLSDYTTAGACAFSI
                     GISALVSYRITKGSSMFVKQAGLLAVLWSETLATTMITANIVKSSVQRTRPYVYNSNV
                     PLEKRMEYDGRKSFFSGHTTFAASNTFFAAHVVSQYFPKTWLSYSTWAVAGLLPASVA
                     YMRVDSGRHFPTDVITGYIVGAACGIVIPHLHTLQNKQNATVHMGISPQTISFVVRL"
     gene            complement(1451..>2760)
                     /locus_tag="P5243_05510"
     CDS             complement(1451..>2760)
                     /locus_tag="P5243_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRS18938.1"
                     /translation="DYESDSYMKIIEEVSALSEQFYSIEEINIDAEVEKILIGLGFNR
                     SDFDRPTGDFSGGWRMRIELAKILLQNPDLILLDEPTNHMDIESVQWLEDFLITNGKA
                     VMVISHDRAFVDAITNRTIEVTMGRIYDYKVNYSSYLQLRKERREQQQKHVDEQQKMI
                     AETTEFIERFKGTYSKTLQVQSRVKMLEKLVILEVDEEDTSSLKLSFPPCMRSGNYPV
                     IAEHVSKSYGNHLVFQNANFTIARGEKVAFVGKNGEGKSTMIQCIMNEINFEGKLTIG
                     HNCQIGYFAQNEASLLDEEKTVYQTIDDVAVGDIRTKIKDILGAFMFGGESWTKKVKV
                     LSGGERTRLAMIKLLLEPVNLLILDEPTNHLDMKTKDILKEALLNYDGTLILVSHDRD
                     FLDGLVSKVFEFGNKRVKEHLCGIQEFLSLKKMEHLRELERKK"
BASE COUNT          892 a          443 c          415 g         1010 t
ORIGIN      
        1 ctatattgta atctctgatt ttaggtagaa taatagtctt aaacctttgt aggtgttatg
       61 ttttatttgg tattctctct aaaacaaata aattatccat taaaaattat aaaacataga
      121 cgcaagctat aatcctaata atatattgaa ttgtatataa ctgtttttta ttttttgagg
      181 attgacgtta tgtatttcaa aattagggag gcaatagtga caaattaatt tgtaacatat
      241 atattaccat gatttttata agaatttaca tcgtgactat caaaaatctt tactaaagca
      301 aaaagctaaa tactagcgaa gtaatgaaga atcgggaggc acgaaataaa aagaaaaagg
      361 aagaaaaatg agataaaaaa ataccccata aatgatattt tatggggctt atagtaccga
      421 aggtgggaat cgaacccaca cgagattgct ctcactggat tttgagtcca gcgcgtctac
      481 caattccacc acttcggctt attttggagg tgcaaatata catttctctg tttgttatac
      541 aaagttttta tataattttt ttaatcatat aataatacaa aataaatatc tgtatttttg
      601 aagaaaatat gtattatgat atgattagaa gtatatatgt agtaggttta ttttttattg
      661 tactttttta caattcttat tctcaaaaca caagtcctta ttcattaacg ttgaaacatg
      721 aatctgtata tgcagcctat actatatccg gattatcggt aatctatttg ttaaataata
      781 aattaaatac tcatacagaa gaagaaatat caaatttgaa tgcacaagat atttggaaaa
      841 tagatagaaa tacagtaaca aagtattctg ataaaactgc tcttttgagt gattatacta
      901 ctgccggtgc ttgtgctttt agtataggaa tttctgccct tgtatcgtat agaataacaa
      961 aaggaagttc tatgtttgta aaacaagccg gattacttgc tgttttatgg tcagaaactt
     1021 tagcaactac tatgattacg gctaatattg tgaaatcaag tgtacagcgt actcgccctt
     1081 atgtttataa ttcaaatgta ccacttgaaa agagaatgga atacgatgga agaaaatctt
     1141 ttttttcggg acatacaaca tttgctgctt ctaatacatt ttttgcagct cacgttgtgt
     1201 cacaatattt tccaaaaact tggttgagtt atagtacatg ggctgttgca ggtttgttac
     1261 cggcttcggt agcttatatg cgtgtagatt caggcagaca ttttcctacc gatgtaatta
     1321 caggttatat tgttggtgca gcatgcggta ttgtaatacc tcatttacat acgttacaaa
     1381 ataaacaaaa tgcaactgtt cacatgggaa tatcgccaca aactatatct ttcgttgttc
     1441 gattgtaatg ttattttttt cgttccaatt cacgtaaatg ttccattttt ttgagtgata
     1501 aaaattcttg aatgccgcat aaatgttctt ttacacgttt attcccaaat tcaaaaactt
     1561 ttgatactag tccatcaaga aaatctcggt catgtgatac taaaataaga gttccatcat
     1621 aattgagtaa tgcttctttt aaaatatctt ttgttttcat gtctaaatga ttcgttggtt
     1681 cgtcaagtat cagtaaattc accggttcaa gtaataattt tatcatagct aatctggttc
     1741 gttcacctcc ggaaagaact tttacttttt ttgtccaact ttcacctcca aacataaatg
     1801 ctccaagtat atctttaatt ttagtgcgaa tgtctcctac agcaacatca tcaatagttt
     1861 gatacacggt tttttcttca tcaagtaaac ttgcttcatt ttgagcaaaa tatccaattt
     1921 gacaattatg tccaattgtt aatttccctt caaaattaat ttcattcatt atacattgta
     1981 tcatggttga tttaccttca ccattttttc caacaaaagc aactttttcg cctcgagcta
     2041 ttgtaaaatt agcattttga aatactaagt gattgccgta cgattttgaa acatgttctg
     2101 ctattacagg gtaatttccc gagcgcatac agggaggaaa tgaaagtttt aatgatgaag
     2161 tatcttcttc gtcaacttca agaatgacta atttttcaag cattttaact cgtgattgca
     2221 cttgcaatgt tttagaatat gtacctttga accgttcaat aaattcggta gtttctgcaa
     2281 tcattttttg ttgttcatct acatgttttt gttgttgttc tcttcgttct tttcgcaatt
     2341 gtaaatagct tgaataatta actttatagt cataaattct gcccattgtt acttctatgg
     2401 tacgattagt aatagcatct acaaatgctc tatcgtgcga aattaccatt actgctttac
     2461 catttgtgat aaggaaatct tctaaccatt gtacagattc tatatccata tggtttgtag
     2521 gttcgtcaag aagaatcaaa tcaggattct gtaataatat tttagctaat tctattcgca
     2581 ttcgccaacc tccactaaaa tcaccggttg gtctgtcaaa atctgaacga ttaaaaccta
     2641 atccaataag tattttttca acttctgcat caatatttat ttcttctata gaataaaatt
     2701 gttcacttaa agcagaaacc tcttcaatga ttttcatgta tgaatctgat tcgtaatcag
//
LOCUS       DAOTOW010000258         2759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28621_length_2759_cov_3.640902, whole genome shotgun sequence.
ACCESSION   DAOTOW010000258 DAOTOW010000000
VERSION     DAOTOW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28621_length_2759_cov_3.640902"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..897
                     /locus_tag="P5243_05515"
     CDS             214..897
                     /locus_tag="P5243_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRS18939.1"
                     /translation="MKHIVVLTGAGISAESGIKTFRDAGGMWEEYDIMDVASIDGWFK
                     NPALVLDFYNKRRADLEHAQPNKAHLLLAQLEQHAKVSIITQNVDNLHERAGSSSVLH
                     LHGELTKVRSTANDFDVQDIGYKPIHIGDVCSQGYQLRPHIVWFGEPVPLIQDAAKIT
                     SKADVLIIIGTSMQVYPAAGLLHYAPASIPIYFIDPNPAIESTKQITVIAEQASSGMQ
                     KVFDELLQQ"
     gene            944..>1155
                     /locus_tag="P5243_05520"
     CDS             944..>1155
                     /locus_tag="P5243_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18940.1"
                     /translation="MTNSKKRVVVLCGAGAINPWGGIQTSELTKKVLESSSRFLCMDN
                     TTPILQYIYNILKNNGLHENEINFETL"
     assembly_gap    1156..1165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1166..2267
                     /locus_tag="P5243_05525"
     CDS             <1166..2267
                     /locus_tag="P5243_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRS18941.1"
                     /translation="INCVDELIVHYSYSNKKEKKQSIHKSIFQVINEDFINNFDVESS
                     NGDTFKLIYPKGEKTDYEKPSYNNESPEQFYYIQLLNNILTSINSAVSNYSYHSPNNS
                     NIIKEKNKTVNENFQKWVTQICDCNILRIYTLNYDRVFKILTKEINIDLFEGFTYDDE
                     IPVSGILPDIKKIITDFESNVHYNLHGSIFWNVNNPELPYRNFLPFITLHPYPKLNVN
                     NPITACIQSEKGNSMVISNIVTGFQKIQKTAVAPFKQMQYSFDKDCFIADEIVIVGYS
                     FGDNHINYSLKLALQYNQNIRITLIDPCYSNKDGYDKLIKNIINIIPEIAINKELSLK
                     YSNNNKTCEYFDSKLIVHSMGFDEYLEIYSNL"
BASE COUNT         1029 a          366 c          422 g          932 t
ORIGIN      
        1 taagttacaa atttacactt atttttcctg tcctaatttt ggggtgtatt atataatatt
       61 tcaggaatat ttgtgagcat tttaatcgca atttaatgat ttgtaaatat atgaatatta
      121 atttttttta tgaatactag aaaaagaaat atctttgata cactattaat aaaaactaat
      181 taaaaagaca ttcagacttt taagacatac aacatgaaac acatagtagt actcacagga
      241 gcaggaatca gtgccgagtc gggaatcaaa acatttcgcg atgccggtgg catgtgggaa
      301 gaatatgata ttatggatgt ggcaagtata gatggatggt tcaaaaatcc ggcgttggtg
      361 ttggactttt ataataaacg aagggcagat ttggagcatg cacaaccaaa caaagctcac
      421 ttgttgttgg cacaattaga acaacatgcc aaagtatcta ttattaccca aaatgttgac
      481 aatttgcacg agcgagcggg gagttcctca gtactgcatt tgcatggcga gttgacaaaa
      541 gttcgcagta ctgccaatga ttttgatgtg caagatattg gatataaacc tattcacatt
      601 ggcgatgtct gttcgcaagg ctatcaatta cgacctcata ttgtttggtt tggcgaaccg
      661 gtaccactta tacaagatgc ggcaaagata acttctaagg ccgatgtgct tataataatt
      721 ggaacatcta tgcaggtata ccctgcagca ggattattac attatgcacc ggcatctata
      781 cctatatatt tcatagaccc caatccggca atagaatcaa ccaagcaaat tactgtaatt
      841 gccgaacaag caagtagtgg tatgcagaag gtgtttgatg aactgttgca acaataacat
      901 gtattcatct tagatatctt ttttaaatat taattgagat aatatgacta atagtaaaaa
      961 acgtgttgtt gttttatgtg gtgcaggtgc aattaatcct tggggtggta ttcaaacctc
     1021 cgaattaact aaaaaggttt tagaatcatc atcacgtttt ttatgtatgg ataatacaac
     1081 tcctatatta caatatattt ataatatact aaaaaataat ggtttacatg aaaatgaaat
     1141 caactttgaa acactnnnnn nnnnnaataa attgtgttga tgaattaatt gtacattatt
     1201 cttattcaaa taaaaaagaa aaaaagcaat caattcacaa atcaattttt caagtcataa
     1261 atgaagattt tataaataat tttgatgtcg aaagtagcaa tggagataca tttaagctaa
     1321 tttatcctaa aggagaaaaa actgattacg aaaaaccgtc atataataat gaatcacctg
     1381 aacaatttta ttatatacaa ctattaaata atatccttac atcaatcaat agtgctgtat
     1441 ctaactattc atatcactct ccaaataatt caaacataat taaagaaaag aataaaactg
     1501 ttaatgagaa ttttcaaaaa tgggtaactc aaatatgtga ttgtaatatt ttaaggattt
     1561 atactttaaa ttatgataga gtctttaaaa ttctaactaa agagattaat atagatttgt
     1621 ttgaaggttt tacttatgat gatgaaatac ctgtaagcgg tatattacct gatattaaaa
     1681 aaattattac agattttgaa tcaaatgttc attataattt acatggatca attttttgga
     1741 atgtaaataa tccggaatta ccatatagaa attttttacc ttttattact ttgcatccgt
     1801 atccgaaatt aaatgtaaat aatcctatta ctgcatgcat acaatcagaa aaagggaata
     1861 gtatggtaat ttctaatata gttacaggat ttcaaaagat acaaaaaaca gcagttgccc
     1921 catttaaaca aatgcaatat agttttgata aagattgttt tattgctgat gaaattgtta
     1981 ttgttggata ttcttttgga gataatcaca ttaattatag tttaaaacta gctttgcaat
     2041 acaatcaaaa tattagaatt accttaatcg acccttgtta ttctaataaa gatggatatg
     2101 ataagttaat taagaacata attaacataa tacccgaaat tgcaattaat aaagaacttt
     2161 cattaaaata ttcaaataac aataaaacat gtgagtattt tgattcaaaa ttaatagttc
     2221 attctatggg gtttgatgaa tatttggaga tatatagtaa tttataattc acaattattc
     2281 gttaaaaata taaaatatta agaataccga attacgggtt tactataagc cattcaatta
     2341 aggaatcgtt aaaaataatg aagttaatgc gtagatttag cagtttgccc aacactttgt
     2401 tttatattta tttacaatgc tttcatgggc attttgttaa atcagcatta acaaatatat
     2461 ttttagatta cgtattgcat ggcggcactt ttgattactt ttttgctgca gaaaaagtaa
     2521 taagaagtat ataaaaaact aagcgtttta ttttttgcta aaatagtaat aataagacct
     2581 gtctagtaat tctccaattt tttttcggac atcagtgctt gtaattattt ttttttttca
     2641 tataattctg tttcgtaaca aaataacaga ataatgaaaa taaatacaca aatggttaat
     2701 gaactaaatc attaatggag tggaacatgg ttggaaaaga agctcaacgc cttttcaaa
//
LOCUS       DAOTOW010000259         2752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28726_length_2752_cov_3.851687, whole genome shotgun sequence.
ACCESSION   DAOTOW010000259 DAOTOW010000000
VERSION     DAOTOW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28726_length_2752_cov_3.851687"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="P5243_05530"
     CDS             complement(<1..430)
                     /locus_tag="P5243_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRS18942.1"
                     /translation="MAQSSFGNYFEDFTEGEVIEHTLSKTIFESDNNLFSLLTMNHHP
                     VHTNIDYAAQNQHGKILVVGTLVFSLAVGITVPDISGKAIANLGYETIEHVNPVFIND
                     TIYAKTTVLKTTESKSKPDRGIIYVETIAYNQHKQEVLKFR"
     gene            complement(435..851)
                     /locus_tag="P5243_05535"
     CDS             complement(435..851)
                     /locus_tag="P5243_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HRS18943.1"
                     /translation="MKRITVYDCSVIELPKIHNRAGNITPVHGNREIPFDIQRIFYLY
                     DIPGGESRGAHAHKECHQFLIAASGAFEIDLDDGITKRTVSLNRPNFGLHIPPGIWAA
                     EKGFSSGAICLVLASHTYNEADYIRKYEDFLAYIKQ"
     gene            complement(848..1258)
                     /locus_tag="P5243_05540"
     CDS             complement(848..1258)
                     /locus_tag="P5243_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HRS18944.1"
                     /translation="MEITECKLIDLPKIQDPRGNLSFIEGGKHIPFAVERTYWIYDVP
                     GGQMRGGHAYTNLHECIIALSGSFDVVINNGKETKTISLNRSYYGLYVPPMIWRSLEN
                     FSTNSLCLILSSAPYSEDLYIRNYDDFLKQATGV"
     gene            complement(1239..1955)
                     /locus_tag="P5243_05545"
     CDS             complement(1239..1955)
                     /locus_tag="P5243_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18945.1"
                     /translation="MPYRRLPNTDAARIRALTNAINKTMSMHPDDIAFSYKVLQSAKF
                     FLPTFKTGINYQRADYSQSIEKNSNYNSLQKRAKMYISHFIQVLNMAIARDEIPADAR
                     TFYGLEKYTKKLPPLTNDDEIIAWGKKLIDGEEARVQQGGNPMVNPRIAMVKIQYDKY
                     VNAYRSNSFIQSKNERAVEYVQELRQKADDIILTIWNEVEQTFAHLPAAEKRKKAEEY
                     GLVYVFRKHEKPEDDGNNGM"
     gene            2257..>2752
                     /locus_tag="P5243_05550"
     CDS             2257..>2752
                     /locus_tag="P5243_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRS18946.1"
                     /translation="MQELRNIAIIAHVDHGKTTLVDRILHQVKTFRDNEEVGELILDN
                     NDLERERGITILSKNVSVRYKGVKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLEKAIELHLKPIVVVNKVDKPNCDPEGANEAVFDLMCNLNASEDQLDFK
                     TVYGS"
BASE COUNT          845 a          457 c          514 g          936 t
ORIGIN      
        1 gtcgaaattt taatacttct tgtttgtgtt gattgtaagc aatagtttct acatatataa
       61 ttccgcggtc gggtttcgat tttgattcgg tggttttaag tacggtggtt ttggcgtaaa
      121 tggtatcgtt tataaatacc ggattcacat gttcaatggt ttcgtatcct aaatttgcta
      181 tggctttgcc actaatgtcg ggtacggtaa tgcctacagc caaactaaac actaatgtgc
      241 ctactactaa tatttttccg tgttggtttt gggcagcata gtctatattg gtatgcaccg
      301 ggtgatgatt catggtaagc aagctaaaca agttattgtc gctttcgaaa atggtttttg
      361 acaatgtatg ttctataact tcaccctcgg tgaaatcttc gaaataattt ccaaatgaac
      421 tttgagccat agaattattg tttaatgtaa gctaaaaaat cttcgtattt gcgaatataa
      481 tcggcttcgt tatatgtatg cgatgccaat accaaacaaa ttgctcccga cgaaaaacct
      541 ttttctgctg cccaaattcc cggaggtata tgtaatccaa aatttggtcg attcagactt
      601 acagtacgtt ttgttattcc atcgtccaaa tcaatttcga atgctcccga agcagcaatt
      661 aaaaactggt gacattcttt gtgtgcatgt gcaccgcgcg attcgccacc cggaatgtcg
      721 tacaaataaa atatgcgttg tatgtcaaag ggtatttctc tgttgccatg cacaggagta
      781 atatttcctg ctctgttatg aatttttgga agttctatta ccgaacaatc atatactgtt
      841 atacgtttca tacaccggta gcttgtttta aaaaatcatc gtaattgcga atatacaaat
      901 cttcggaata tggtgccgac gacagaatga ggcataagga attcgttgaa aaattttcga
      961 gcgaacgcca tatcatagga ggcacataca atccgtagta agaacgattg agtgatattg
     1021 ttttggtttc tttaccgtta tttattacca catcaaaact tcccgaaaga gcaataatac
     1081 attcgtgtaa attggtatag gcatgtccgc cgcgcatttg accacccggc acatcatata
     1141 tccaataggt gcgttctacc gcaaagggta tatgcttgcc tccttctata aacgacagat
     1201 taccgcgagg gtcttgaatt tttggtaaat ctataagttt acattccgtt atttccatcg
     1261 tcttcgggtt tttcgtgttt tctaaataca tatacaagtc cgtattcttc agcttttttt
     1321 cgtttttcgg cagcaggcaa atgggcaaat gtttgttcta cttcgttcca gatagttaga
     1381 ataatatcat cagctttttg tcgtaattct tgcacatatt ccacagcacg ttcgtttttc
     1441 gattgtataa aactattgga acgatatgca tttacatatt tatcgtattg tatttttacc
     1501 attgctatgc gtggatttac cattgggtta ccaccttgtt gtacccgagc ttcttcaccg
     1561 tcaattagtt ttttgcccca tgctataatt tcatcatcat tagtaagagg aggtagtttt
     1621 tttgtgtatt tttcaagccc gtaaaatgtt cgagcatcgg caggaatttc gtcgcgagct
     1681 atagccatat ttagcacttg aataaagtgc gaaatgtaca ttttagcacg tttttgcaat
     1741 gaattatagt ttgaattttt ttctattgac tgagaataat cagctcgttg ataattgatt
     1801 cctgtcttaa atgtgggaag aaaaaattta gcactttgca aaactttgta tgagaaagca
     1861 atatcatcgg ggtgcatact catagtttta tttatggcat ttgtaagtgc tcgtatacga
     1921 gcagcatctg tatttggcaa tcgtcggtaa ggcatagttt atatagtatt tacacaaaca
     1981 tttataatag aatacacaca taaatgcgaa tgattcacaa atataggtat aaatattatt
     2041 cgcatatata tgatttatta taaagttata aaccacatat gttgaaatgt ttttattgaa
     2101 tttagagagt aaagagtaat atttgtgagt tttaaaggga aagtaacaaa ttatacaaac
     2161 aaaatagctg ataatacgtg cttgaaaaac aaaaaattaa tcgaaatttg cagccccttg
     2221 ttcatgcaag gatatgtatc aatttttaac taatatatgc aagaattacg taacattgct
     2281 attatagcac acgttgacca tggtaaaaca actttggtag atagaatttt acatcaagta
     2341 aaaacattta gagacaatga agaagttggt gaattaattt tagataataa cgatttagaa
     2401 cgcgagcgag gaatcactat tttatcaaaa aatgtatcgg tacgatacaa aggagttaaa
     2461 ataaatataa ttgatactcc cgggcactct gattttggcg gagaagtaga acgcgttcta
     2521 aatatggccg atggagtatt gcttttagta gatgcttttg aaggacctat gcctcaaaca
     2581 cgttttgttt tagaaaaagc tatagaatta cacttaaaac ctatagttgt agtaaacaag
     2641 gttgataaac ctaattgtga tcccgaaggt gccaatgaag cggtttttga cttaatgtgt
     2701 aatttgaatg caagtgaaga ccaacttgat tttaaaacag tatatggttc at
//
LOCUS       DAOTOW010000260         2747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28809_length_2747_cov_2.768574, whole genome shotgun sequence.
ACCESSION   DAOTOW010000260 DAOTOW010000000
VERSION     DAOTOW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28809_length_2747_cov_2.768574"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="P5243_05555"
     CDS             complement(<1..468)
                     /locus_tag="P5243_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRS18947.1"
                     /translation="MEEYSKDKEILDDLQHKDYKYGFSSNVETDIFPPGLNEDIIRRI
                     SAVKEEPKFLLEWRLKAYRHWLTMEHPKWAHISFPMPDFQSISYYAQPSNKPKYESLD
                     EVDPEILATFNKLGIPLEEQKILSGVAVDAVFDSVSVHTQFREQLQEKGIIFCS"
     gene            complement(533..1243)
                     /locus_tag="P5243_05560"
     CDS             complement(533..1243)
                     /locus_tag="P5243_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS18948.1"
                     /translation="MDIKIYFDDVSVGYNIQEPVLEHVSFTVQQGELVYLIGKTAAGK
                     SSLLKTMYAEIPPLAGKVLVADYDMNEIKKSKIPFLRRKVSVIFQDFQLLPDRNVHEN
                     LLFVLKATGWTKPIEMQERINEVLTQVGLFDKEYKMLHQLSGGEQQRVVIARALLNKP
                     EIILADEPTGNLDPDSSDEIFKLLHSIAQSGCAMFVVTHNYQLVKKYTGRTLQCTNNT
                     ITEIFEQEHAIDFGALME"
     gene            1870..>2747
                     /gene="nadA"
                     /locus_tag="P5243_05565"
     CDS             1870..>2747
                     /gene="nadA"
                     /locus_tag="P5243_05565"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRS18949.1"
                     /translation="MIYNEMINELGFIQEKPDSNINLKEEILRIKKEKNAIICAHFYQ
                     TDEIQEIADYIGDSLKLAQIAENNTADIIVLAGVHFMAETAKILAPHKKVLIPDLNAG
                     CSLAESCPYDDFKAFKEAHPNHIVISYVNTTAAIKTLTDVVCTSSNAEQIVNSFPKNQ
                     KIIFGPDRNLGRYISQKLNREMVIWDGACTVHEKFSLEKILQLKQEYPNAKILAHPEC
                     TQPILMISDYIGSTSNMLDFSKQDSANEYIVATESGILFKMREASPAKTFISAPPNDS
                     TCACNDCEFMKLITLE"
BASE COUNT          944 a          430 c          381 g          992 t
ORIGIN      
        1 tgaacagaaa attattcctt tttcttgtaa ttgttcacga aattgagtgt gcaccgatac
       61 gctatcaaac acggcatcta cggcaactcc cgacaaaatt ttttgttctt cgagcggaat
      121 tcctaattta ttaaatgttg ccaatatttc agggtcaacc tcatcaagac tttcatattt
      181 tggtttatta cttggttgag cgtaataact tatactttga aaatcaggca ttggaaacga
      241 tatatgagcc cattttggat gctccatagt aagccaatga cgataggctt taagccgcca
      301 ttctaacaaa aatttgggtt cttcttttac tgctgaaata cgacgaataa tatcttcatt
      361 taaacccggc ggaaatatat cggtttcaac attagaacta aatccatact tataatcctt
      421 gtgttgtaaa tcgtcaagta tttctttatc tttcgaatat tcttccatgt atttttaagt
      481 tttgtattat gttagactat tacaaaagtc aaaatttaca caatacatca ttttattcca
      541 ttaatgcccc aaaatctata gcatgttctt gctcaaatat ttcagttatt gtattgtttg
      601 tacattgtaa tgtgcgtccg gtatattttt ttactaactg ataattatgc gtaacaacaa
      661 acatagcaca acccgattga gcaatagaat gcaagagttt aaaaatttcg tcggaactat
      721 cagggtcaag attacctgta ggttcgtcgg ccaatataat ttcgggtttg ttgagcaatg
      781 cacgagctat tacaacgcgc tgttgttcgc caccggaaag ttgatgaagc attttatact
      841 ccttgtcaaa caatccaact tgtgtaagca cttcatttat tcgctcttgc atttcaatgg
      901 gtttggtcca accggttgcc ttaagtacaa ataacaaatt ttcatgtaca ttacgatcgg
      961 gcaataattg aaaatcttga aatattactg aaacttttct gcgaagaaac ggtattttag
     1021 attttttaat ttcattcata tcataatcgg ctactaaaac tttgcccgcc aaaggtggaa
     1081 tttctgcata cattgtttta agcaaactcg acttgcctgc agcagtttta ccaataagat
     1141 ataccaattc accttgttgt acagtaaaac tgacatgttc caaaaccggt tcttgtatat
     1201 tataccctac tgatacatca tcaaaatata tttttatatc cattcctgat tgtattttat
     1261 aataagcaac aaatatacta ttattcaagt ataattctat aagtaaatta acggaaagtt
     1321 tttctataca agtacttaat agatacatat ctaattagta tcatatctat attctattta
     1381 cttataatta ccattgttaa tatcataact cattattaat ttagtattta catttttttg
     1441 tttttactaa tatttgattt acaattcatt aacaaatctc ataaattgtt aataatacgc
     1501 atatttttgt gtatagattt acgaaagaat tcgtctaaaa aatttggtag tttcaattgt
     1561 tgtttgcatt aatttttttt tcgtgtcatc caaatatagt tttcattttt ctttagaatt
     1621 tgttaacata taaattcaca tgaaataatg tgtaaaatta ccaacattat agtgtttata
     1681 attcattcac atgtttgtta ataaattgta aaactcattc attctaacta ttttataatt
     1741 ttatagcgta tttataacat attagcaaac tgtttttatg ataaaaacaa tacttttgta
     1801 aaaaaaatat gaacctattc acagcacatc atcaacatca tatttttttt atttaaattt
     1861 atatatatta tgatttataa tgaaatgatt aatgaattag gatttataca agaaaaacct
     1921 gattcaaata taaatctcaa agaagaaatt cttcgtatta aaaaggagaa aaatgcaatt
     1981 atttgtgcac acttttatca aaccgatgaa atacaagaaa ttgccgatta tataggtgat
     2041 agtttaaaat tagcgcaaat tgccgaaaat aatactgcgg atattattgt attagctggt
     2101 gtacatttta tggcagaaac tgcaaaaatt ttagctcctc ataaaaaagt tcttattccc
     2161 gatttaaatg caggatgttc gttagccgaa tcgtgtcctt acgacgattt taaagcattt
     2221 aaagaagcgc atccaaatca tattgtaatt tcatatgtaa atacaacagc ggctattaaa
     2281 acacttacag atgtagtatg tacttcatct aatgctgaac aaattgtaaa ttcatttcca
     2341 aaaaatcaaa aaattatatt tggacctgac agaaatttag gaagatatat ttctcaaaag
     2401 cttaatagag aaatggttat ttgggatgga gcgtgtacag tacatgaaaa attttcgtta
     2461 gaaaaaatat tgcagcttaa acaagaatat ccgaatgcta aaattttggc tcatcctgaa
     2521 tgtacacaac ctattcttat gataagcgat tatataggtt ctacttcaaa tatgcttgat
     2581 ttttcgaaac aagattcagc aaatgaatat atagttgcaa cagaatcagg tattttattt
     2641 aaaatgcgtg aagcaagtcc tgctaaaact tttatttctg ctccaccaaa tgattctacg
     2701 tgtgcatgta acgattgtga atttatgaaa ttgattacct tagaaaa
//
LOCUS       DAOTOW010000261         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28925_length_2738_cov_3.617965, whole genome shotgun sequence.
ACCESSION   DAOTOW010000261 DAOTOW010000000
VERSION     DAOTOW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28925_length_2738_cov_3.617965"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="P5243_05570"
     CDS             <1..426
                     /locus_tag="P5243_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRS18950.1"
                     /translation="ETQFMFVDNAAMQIIQWPKNFDVMVTENMFGDILTDEASVITGS
                     MGLLPSASIGIHTSVFEPIHGSYPQAAGKNIANPVATILSAAMMFEYAFGMMQEGALI
                     RKAVAASLEANVVTEDISKEHAKSTSDVGDWITQYIEKA"
     gene            493..1347
                     /locus_tag="P5243_05575"
     CDS             493..1347
                     /locus_tag="P5243_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitosanase"
                     /protein_id="HRS18951.1"
                     /translation="MFITHFSSRIVILRNILVFIITVCVYTYCVKKGPEPVEDNLIPF
                     DKAHKIIAEKIISCFENSTPIIQYDYIEYLNDGRGYTAGKAGFTTATGDMLQLIELYT
                     DSVPTNNLAYFIPTIKEYSQHENNSIKGLENLPKAWKQACKDSVFIHCQDIIADSLYY
                     IPAVRIAKQLGLRYPLSLLNIYDAIIQHGEGDDPDGLPAIIHKTNTKCGGYPLQGIHE
                     YIWLETFIDIRKSIVKHAHNADTRKEWRESVDRVTALDKLRKEGNFLLQGTIEIKVYG
                     DTFKITYK"
     gene            1474..2568
                     /locus_tag="P5243_05580"
     CDS             1474..2568
                     /locus_tag="P5243_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HRS18952.1"
                     /translation="MTTETTKNKGWLVVFAGVAINLALGILYSWSIFKDSISKSIDTG
                     GDGAFTWSKAALNDPYAVCILVFAFSMILAGKIQDKYGPRVTALIGGVLVGIGFFILS
                     STTSYALWVLGFGVLAGMGIGFGYSAATPPALKWFPSSKTGVVAGIVVSGFGLAPVYI
                     APLATYLVNNFGLQHAMLIFAILFILVVCGAALLLQNPPAGYIAHDKSNTDTQTTKPI
                     ASKDFTVKEILKTPSFYLIWVIFFIASGAGLMVIGSISGMAKKSMGEAAFVAVAIMAI
                     GNASGRIIAGLVSDKIGRVLTLLIIIVFQASLMFASMLLIGGNTEALLIVLLATFIGF
                     NYGTNLSLFPSFTKSFWGMKNFGVNYGVLM"
     gene            complement(2550..>2738)
                     /locus_tag="P5243_05585"
     CDS             complement(2550..>2738)
                     /locus_tag="P5243_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18953.1"
                     /translation="FYYAVICFFSICFKTSANNAHTNKHEPAIIKEVVCVPVFATMVC
                     DNRLKINPPNPHAYIKTP"
BASE COUNT          941 a          439 c          492 g          866 t
ORIGIN      
        1 gaaacacagt ttatgtttgt tgataatgcc gcaatgcaaa ttattcaatg gccaaaaaat
       61 ttcgacgtta tggtaaccga gaacatgttt ggcgatattt taaccgacga agcttctgtt
      121 atcacaggtt ctatgggatt actaccatca gcttctattg gtatccatac cagcgttttt
      181 gagccaattc acggttcgta tccacaagca gcaggtaaaa atatagcaaa cccggtggca
      241 actatacttt cggcagctat gatgtttgaa tacgcattcg gcatgatgca agaaggtgct
      301 ttgattcgta aagcagtagc agcctcatta gaagcaaatg ttgtaaccga agatatatcg
      361 aaagaacatg caaaatctac cagtgatgta ggcgattgga ttacacaata tattgaaaaa
      421 gcataattgc ttatctataa aaaggctgtt ttctgtttag agagcagcct tttttatgaa
      481 tacttacaaa gtatgttcat tacacatttt tcttcacgta tagttatttt acgaaatata
      541 cttgtattta ttatcacagt atgtgtatac acatactgtg taaaaaaagg ccctgaacca
      601 gttgaagaca atttaattcc ttttgataaa gctcacaaaa ttattgcaga aaaaataatt
      661 tcatgttttg aaaacagcac accaataata caatatgatt atatagaata cttaaacgat
      721 ggaagaggtt atacagcagg caaagccggt tttacaactg ctaccggcga tatgttacaa
      781 cttatagaat tatataccga ttctgttccc accaataatc ttgcgtactt cattcccaca
      841 ataaaagaat attcacaaca tgaaaataat tctattaaag gtttagaaaa tttacctaaa
      901 gcatggaaac aagcatgtaa agattctgtt tttatacact gtcaagatat aattgccgat
      961 tcactatatt atatacctgc tgttcgtata gcaaaacaat taggattaag atatccatta
     1021 tcattactca atatttacga tgctatcatt caacatggcg aaggtgacga ccccgatggg
     1081 cttcctgcta ttatacacaa aacaaatact aaatgcggtg gatatccttt acaaggaata
     1141 catgaatata tatggttaga aacatttatt gatatacgta aatctattgt aaaacatgct
     1201 cataatgctg atactcgaaa agaatggaga gaatcggttg atagggtaac cgctttggat
     1261 aaactacgaa aagaaggtaa ttttttactc caaggtacca tagaaattaa agtttacgga
     1321 gatacattta aaattacata taaataattc atttttttga gtttaaaagg aatttagacg
     1381 catactaaca atgatatatg tgatatacat cacatttaca cctataaaaa caaaaaaaag
     1441 tataatattg cagcaaaaca taaaaacagt aatatgacaa cagaaacaac aaaaaacaaa
     1501 ggatggttag tggtttttgc aggagtagcc attaatcttg cattaggcat tttatactct
     1561 tggagtatat ttaaagacag tatatcaaaa tcaattgata caggaggcga tggtgctttt
     1621 acatggtcaa aagcagcatt aaatgacccg tatgcagtat gtattttagt ttttgccttt
     1681 tcaatgatat tggccggcaa aatacaagat aaatatggtc cacgtgttac tgctcttata
     1741 ggaggagtat tggtaggaat aggatttttt atattatcat caaccacaag ttatgcactc
     1801 tgggtattag gttttggagt attggcaggt atgggaatcg ggtttggata ttcggcagca
     1861 acaccacctg cattaaaatg gtttccatct tctaaaacag gtgttgtagc aggtatagta
     1921 gtatcgggct ttgggttagc tccggtttac atagctccat tagctacata tttggtaaat
     1981 aactttggat tacaacatgc catgcttata tttgcaatac tatttatact ggttgtttgc
     2041 ggagcagcat tactccttca aaatccacct gcaggttata tagctcatga taaatccaat
     2101 acagatactc aaacaactaa accaattgca agcaaagatt ttaccgttaa agaaattctt
     2161 aaaacacctt cattttatct tatttgggtt atattcttta tagcttcggg agcaggatta
     2221 atggtaatag gaagcatatc gggcatggct aaaaaaagta tgggcgaagc agcttttgtt
     2281 gcagttgcaa ttatggctat aggtaacgca tcgggacgaa ttattgccgg tctggtatca
     2341 gataaaattg gaagagtact tacattatta attataattg tatttcaagc aagccttatg
     2401 tttgcatcta tgttgcttat aggcggaaac actgaagcat tacttatagt attacttgcc
     2461 acgtttatag gattcaatta cggcaccaat ctttcattgt ttccatcatt taccaaaagc
     2521 ttttggggta tgaaaaattt tggtgtaaac tatggtgttt taatgtaggc atggggatta
     2581 ggaggattta ttttcagtcg attatcacaa accattgtag caaaaacagg aacacatact
     2641 acctctttta ttatagcagg ttcatgttta ttggtgtgtg cattattagc acttgtcttg
     2701 aaacatatag aaaaaaaaca aattacagca taatagaa
//
LOCUS       DAOTOW010000262         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28931_length_2738_cov_2.633619, whole genome shotgun sequence.
ACCESSION   DAOTOW010000262 DAOTOW010000000
VERSION     DAOTOW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28931_length_2738_cov_2.633619"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..532)
                     /locus_tag="P5243_05590"
     CDS             complement(95..532)
                     /locus_tag="P5243_05590"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS18954.1"
                     /translation="MMKHIYVSIILLLSMNYVSYSQTFSDATVKKQHKGPYIEFVTTI
                     HNFGTITFGEPALYSFDFTNTGTESLVISNVKTTCGCTVPQWNSKPIAPGESSSITVE
                     YDTKRSGGFSKGITVFSNAINKQVNLVIEGEVTPNLNKPILGQ"
     gene            complement(652..1632)
                     /locus_tag="P5243_05595"
     CDS             complement(652..1632)
                     /locus_tag="P5243_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRS18955.1"
                     /translation="MILYGSNGTYEYNENIQSEKNEGSLFVSFKGIHKELNRVVVIRK
                     MQEPADSISHNHFEIIHTFLTQIHSIDEGICQVLDVCLYDGHLYIVRLYVEGISLQKL
                     LFTPDFPQYRNSYFMLQIMLQTLSIIEKVHSCGIIHHSICPQHIFIESNEIGKIDMMH
                     PSIKLVAFEYALLHFHSVIPFQKPPVVPTYSAPEVVLKYNNLISPASDLYSLGICLYE
                     CFTRKPAFDTENKDLLLHTQVSYPLKKTWNVPQELFTIIQKATYKHVFRKPPARYTGS
                     ELEQFLKVACSKRYLQASHMKNDIEAFFKTMAVTNKKTIAQTVSQFFRKR"
     gene            complement(1629..2414)
                     /locus_tag="P5243_05600"
     CDS             complement(1629..2414)
                     /locus_tag="P5243_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRS18956.1"
                     /translation="MTNIFKRTELLIGSENMQAITNQRVILFGVGGVGSWCAESLVRS
                     GIQYLTIVDSDTICETNCNRQLHATSSTIGRLKTDVMRERLLDINPMATIIALNKTYT
                     PFNSDEFELHNYDYIIDCIDSLSCKIDLIRKATKTKAVFFSSMGASLKVNPLQVKVAE
                     FWRVRGCPFGSIIRKRIRKGDLPAKKFLCVYSEEVLENKNTHILPESHLEMQPVFEAD
                     NEEQWQSKKAVINGTMVQVTAVFGFTLAGLVIQSILNAQNNTI"
     gene            complement(2424..>2738)
                     /locus_tag="P5243_05605"
     CDS             complement(2424..>2738)
                     /locus_tag="P5243_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS18957.1"
                     /translation="NYSTAVWDSNLESSITIIGEKGTVKVAGQYMNEVVYCNIKDYTM
                     PELAPCNPPNDYGPYKGSAANHHYVIRNVVETLQQKTTITTNAMEGLKVVDIIERIYS
                     YK"
BASE COUNT          896 a          457 c          396 g          989 t
ORIGIN      
        1 gcatcggcaa attttgctaa ttttctaata ggtgatggag gcatagtaaa gcctctgtct
       61 gaaatttttg gcatatatta tttcctcagt aaatttattg tcctaatatg ggtttgttaa
      121 gatttggtgt tacttctcct tctattacaa gatttacttg tttatttatt gcatttgaaa
      181 atacagtaat tccttttgaa aagccaccac ttcgttttgt atcatattct acagtaattg
      241 aacttgattc gccgggagca ataggttttg aattccattg aggtacagta caaccgcatg
      301 tagtttttac gttacttata accaatgatt ctgtacctgt attagtaaaa tcaaaactat
      361 aaagagccgg ctcaccaaat gtaattgttc caaaattatg aattgtagta acaaattcaa
      421 tatatggccc cttgtgttgc tttttaactg tagcatctga aaatgtttgt gaatatgaaa
      481 cataattcat gctcagcaaa agaataatac ttacatatat gtgtttcatc atagcttttt
      541 gtatttaata aactctattc aaaactgtac aaatctaatg taatttttat tattgcatat
      601 cgtaaatact ataaatttgt tgtatataga attacaaaat gcaaaaatta attatcgttt
      661 tctaaaaaat tgagataccg tttgagctat tgttttttta tttgtaactg ccattgtttt
      721 aaaaaatgcc tctatatcgt ttttcatatg actagcttgc aaataacgct tactgcatgc
      781 tactttcaaa aattgctcta attcgctacc ggtataacgc gccggtggtt ttctaaaaac
      841 atgtttatac gttgcttttt gaattatagt aaacaattct tgaggaacat tccatgtttt
      901 tttaagagga tatgaaactt gcgtatgcaa taacaaatct ttattttcag tgtcaaaagc
      961 gggtttacga gtaaaacatt catataaaca aatacccaaa ctatataaat cagatgcggg
     1021 tgaaataaga ttattatatt taagaacaac ttccggtgcc gaataagttg gtactacagg
     1081 aggtttttga aatggaataa cagaatgaaa atgtaacagt gcatattcaa atgccacaag
     1141 ctttattgaa ggatgcatca tatcaatttt tcctatttca tttgattcaa taaaaatatg
     1201 ttgcggacaa atactatgat gaataatacc acatgaatgg actttttcga taatagaaag
     1261 tgtctgcaac attatttgta acataaaata tgagttcctg tactgcggaa aatcgggagt
     1321 aaacaataat ttttgcaaac ttattccttc aacatacaat cgtactatat ataaatgacc
     1381 gtcgtataaa caaacatcta atacctgaca aataccttca tctatagaat gaatttgagt
     1441 taaaaatgta tgtattattt caaaatgatt gtgtgaaata gaatcagcag gttcttgcat
     1501 ttttctaata acaacaaccc tgtttaattc tttatgtatt cccttaaatg aaacaaataa
     1561 agaaccttca ttcttttctg attgtatatt ttcgttatat tcatatgttc cgttacttcc
     1621 gtaaagtatc atatcgtatt gttttgagca ttaagtatag attgaataac aagtccggca
     1681 agcgtaaaac caaaaactgc ggttacttga accattgtac cgttaataac agctttttta
     1741 ctctgccatt gttcttcatt atcagcttca aaaacaggtt gcatttctaa gtgcgattca
     1801 ggtaatatat gtgtattttt attttcaaga acttcttcac tatacacaca caaaaatttt
     1861 tttgcaggta aatctccttt tcgtattcgt ttacgaataa ttgatccaaa tggacaacca
     1921 cgaaccctcc aaaattcagc aacttttact tggagtggat tcactttaag agatgcaccc
     1981 attgatgaga aaaatacagc tttagtcttg gtagcttttc ttatcaaatc aattttacat
     2041 gacaagctat caatacaatc aattatataa tcgtagttat gtaattcaaa ttcatctgaa
     2101 ttaaacggtg tatacgtttt attcaatgct attatagttg ccattggatt tatatcgaga
     2161 agtcgctctc gcatgacatc tgtttttaat ctcccaatag tagatgacgt agcatgcaat
     2221 tgtctattgc aattggtttc acatattgta tcagaatcta caatagtaag atattgaatt
     2281 ccacttcgaa ccaaactttc ggcacaccaa ctgccaacgc cccctacccc aaaaagtatt
     2341 acacgttggt ttgtaattgc ttgcatattt tctgaaccaa tcaaaagttc ggtacgttta
     2401 aaaatgtttg tcattacaaa ctattattta taagaataaa ttcgttctat tatatctacc
     2461 actttaagac cttccatagc attggtagtt atagtagttt tttgttgcaa tgtttctacc
     2521 acatttcgta ttacataatg atgatttgcc gctgaacctt tatatgggcc ataatcatta
     2581 ggaggattac aaggggctaa ttcaggcatt gtataatctt ttatattaca atacactact
     2641 tcattcatat attgtcctgc tactttaaca gttccttttt cgccaattat agtaatacta
     2701 ctttcaaggt ttgaatccca cactgcagta ctataatt
//
LOCUS       DAOTOW010000263         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28932_length_2738_cov_2.617965, whole genome shotgun sequence.
ACCESSION   DAOTOW010000263 DAOTOW010000000
VERSION     DAOTOW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28932_length_2738_cov_2.617965"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="P5243_05610"
     CDS             <1..274
                     /locus_tag="P5243_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRS18958.1"
                     /translation="HYGKRISICSVTNYGVFTPIYGTTQLLSYPGQAISIFSLTPHIP
                     ITLHNLQYPLNQAQLESWWMGTLNTALYDTFTVESDNGICIIFQAF"
     gene            complement(280..1707)
                     /gene="rodA"
                     /locus_tag="P5243_05615"
     CDS             complement(280..1707)
                     /gene="rodA"
                     /locus_tag="P5243_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRS18959.1"
                     /translation="MRNRDADIVKRLDWITIFLYLGLVLAGWANIFAAVYNEEAGSIF
                     DISQKYGKQMLWIIAAFIIGISIIIIDSKFYSGLSYPIYIAVLGLIVLTYLIAPDIKG
                     ARAWIEIGDFRLQPSEFSKLATSLVLARYLSGYGKKITDFSTLFRASLILLIPIMLII
                     LQNDTGSALVYGAFLLVFYRLGMSGWVLVAVVYTVFVFSFSIIYNSFYVSIGIVLLAY
                     GVYWYIARIYKFLPLYTYIVPLLFIVPWVIQYYFVPQLPEFFPFAVGVLVLFVPAIVF
                     IYKKKSKIMFLTYFLTVASLGFTYSVDYIFHDVLGAHQQSRIRVFFGLESDPKGLEFN
                     VIQSKIAIGSGDFWGKGFLQGTQTKNNFVPEQSTDFIFCTVGEEWGFVGSTVVIIAFI
                     LLILRIMYLAEKQRSQFSKIYGYCVASIFFFHFLVNIGMTIGLMPIIGIPLPFFSYGG
                     SSLWGFTMLLFIFIKLDSDRDVLIQ"
     gene            complement(1697..>2738)
                     /locus_tag="P5243_05620"
     CDS             complement(1697..>2738)
                     /locus_tag="P5243_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS18960.1"
                     /translation="LTIVSSPTFDPNLMVGQNVNKYYKILENDPYKPLYNRAIQGDRY
                     PPGSTFKVLNGLIALHEGIITPSFTVSCNGGYFSGGIKVGCHAHPSPVSLLESIQMSC
                     NAYYCTIFRAIIDADKYGSVRNGYEVWRKHMNSMGLGKTLGIDLPSERPGIIPLPTYY
                     DKYYGKNRWKSLTIISLSIGQGEISMTPLQMANVAAIIANRGYFYTPHVVREILDTTM
                     DNSFKQKHVTTIRPEVFEPIIEGMYRVVQGESGATARHVAIPGIPICGKTGTAQNPHG
                     KDHSIFMAFAPRENPKIAIAVYVENAGYGATYAAPIASLLIEKYLTDTIKRPAWEQRM
                     LEMDLIHKTDAK"
BASE COUNT         1002 a          539 c          368 g          829 t
ORIGIN      
        1 acactatggc aaacgcatta gtatatgcag tgttacaaat tatggcgtat ttactcccat
       61 atatggcaca actcaacttt tatcatatcc cggtcaagca atttcaatat tttcgttaac
      121 tccacatatt cctattacat tacacaattt acaataccct ctcaatcaag cacaattaga
      181 atcatggtgg atgggaacgc ttaataccgc attatatgat acttttacag ttgaaagcga
      241 taacggtatt tgtataattt ttcaggcttt ttaattcaac tactgaatta agacatctct
      301 atcagaatct aattttataa atataaacaa aagcatagta aacccccaaa gcgaagaacc
      361 tccataacta aaaaatggca aaggtattcc tattataggc attaatccaa tagtcattcc
      421 tatatttacc aaaaaatgga aaaagaaaat agatgcaaca caatatccat aaatcttact
      481 aaattgtgaa cgttgctttt ctgccaaata catgattcga agtataagca aaataaaagc
      541 aattataact actgtagaac ctacaaatcc ccattcttca ccaaccgtac aaaaaataaa
      601 atcggtactt tgttcgggta caaaattatt ttttgtttgc gtaccttgta aaaaaccttt
      661 accccaaaag tcaccggaac ctattgcaat tttagattgt attacgttaa attcaagacc
      721 tttcggatct gattctaaac caaaaaatac acgtatacgc gactgctgat gcgctcctaa
      781 aacatcatga aaaatataat caaccgaata cgtaaatcct aaactggcaa ctgttaaaaa
      841 ataagtaaga aacataattt tagatttctt cttatatata aaaacaattg caggtacaaa
      901 caaaaccaat actcctacag caaaaggaaa aaattcgggt aattgtggca caaaataata
      961 ctgaattacc caaggcacta taaataataa gggaactata tacgtatata acggtaaaaa
     1021 cttatatata cgcgcaatat accaatacac tccataagcc aataaaacaa tacctattga
     1081 aacataaaaa gaattataaa taattgaaaa agaaaataca aaaacagtat atacaacagc
     1141 cacaagtacc caccccgaca ttcccaatcg gtaaaaaact aataaaaaag caccatatac
     1201 taatgccgaa cctgtatcat tttgaagaat aattaacata ataggtatca acaaaattaa
     1261 agaagctcga aataaggtac taaaatctgt aatttttttt ccatatccgc tcaaataccg
     1321 ggcaagcacc aaactagttg caagtttcga aaattcagag ggttgtaatc gaaaatctcc
     1381 aatttctatc caagcacgtg ctcctttaat atcgggagca ataagatagg taagtactat
     1441 aagcccaagt accgctatat atataggata tgaaagtccc gaataaaact tactgtcaat
     1501 aataataatt gaaataccta taataaatgc agctattatc cataacattt gtttaccata
     1561 tttttgactt atatcaaaaa tacttcctgc ttcttcatta taaacagcag caaaaatatt
     1621 tgcccatcct gcaagaacaa gtcccagata caagaaaatt gttatccaat ctaatcgttt
     1681 tactatatca gcatctctat ttcgcatcag ttttatgaat taaatccatt tctaacattc
     1741 gttgttccca cgccggacgt tttattgtat cagtcaaata tttttcgatt agcaaactgg
     1801 caataggagc agcataggta gcaccatatc cggcattttc cacatacact gctatcgcta
     1861 ttttaggatt ctcgcgcggt gcaaaagcca taaatattga gtggtctttg ccatgaggat
     1921 tttgtgcagt tcctgtttta ccacatattg gaatacccgg aatagcaaca tgacgggctg
     1981 ttgctccaga ttctccttgc acaactctat acataccctc aataataggt tcaaacactt
     2041 cgggacgtat tgttgtaaca tgtttttgtt taaaagaatt atccatagta gtatctaaaa
     2101 tttctcgtac tacatgtggc gtataaaaat atcctctatt tgcaataata gcagctacat
     2161 tagccatttg caaaggagtc atacttattt caccttgtcc aatagacaat gaaattatag
     2221 ttaaagattt ccatctattt ttaccataat atttgtcata ataagttggt aatggaataa
     2281 tcccgggacg ttcacttggt aaatctattc ctaatgtttt tcctaatccc atagaattca
     2341 tatgtttacg ccacacttcg tatccattgc gaacacttcc atacttatcg gcatctatta
     2401 ttgctctaaa tattgtacaa taatatgcat tacacgacat ttgaatcgat tctaagagac
     2461 tcacaggtga tggatgagca tgacaaccta ctttaatacc tcccgaaaaa tatccaccgt
     2521 tacaagaaac cgtaaaactt ggtgtaataa ttccctcatg caatgcaatt aaaccattca
     2581 agactttaaa cgtagaacca ggcggatatc tatcgccttg tattgctcta ttatacaaag
     2641 gtttatatgg gtcattctct aaaattttat aatatttatt tacattttgc ccaaccatta
     2701 aatttgggtc aaacgtaggg ctcgaaacaa ttgttaat
//
LOCUS       DAOTOW010000264         2734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_28980_length_2734_cov_3.624113, whole genome shotgun sequence.
ACCESSION   DAOTOW010000264 DAOTOW010000000
VERSION     DAOTOW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28980_length_2734_cov_3.624113"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="P5243_05625"
     CDS             complement(<1..314)
                     /locus_tag="P5243_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18961.1"
                     /translation="MEAKKIHIGQWIKDVLNEKKISQSELSRRINTTKQNMGKILEKD
                     SLDVKQLFTISQVLDYNFFEPFTKSEPKQNQKSRVILQIEIDENKIEEVLKQIDNKKL
                     YN"
     gene            460..690
                     /locus_tag="P5243_05630"
     CDS             460..690
                     /locus_tag="P5243_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18962.1"
                     /translation="MTNEKLTKEQKHTMFKTWWLAVPNGMVRIYRDQIKKQCGITTVV
                     FYHWFMGNSEIPTLALPIIEKIAGVEVFNENK"
     gene            1080..>2734
                     /locus_tag="P5243_05635"
     CDS             1080..>2734
                     /locus_tag="P5243_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18963.1"
                     /translation="MFVKQEDILSATRGGLDIILHYYPQAQKALTTAKKQFKVRPEKT
                     ASATIKQLTDGNYVVTDFGGDQTPRNGIQVCMYEENCSFKQAIEILAGRHNINGMQKI
                     ERKADYQKRSATADENENDFTVDIKDAMSKFELETLGKHVTAEVCKKYKFYALKSFSR
                     VKNREVHTWLSTDDYPIYMIDGGDYKKILQPFAEKQYRFQWLGENKPKDVLFGMDQLR
                     DAYKKHEELAFAQFNEEEENRKIERMECAIIMSGDRDAINMAAYGYHVLWLNSETAKI
                     STEQIKEISKYVKKIYNVPDIDTTGKREGIKLALTYIDICTVWLPEFLLQLKDSRGYG
                     RKDFKDWVDANENQSRIDIQHKIDKMLNAALCAKFWFEYETEKGLQYEFSNVHGYYFL
                     ACNGFHIIKDLNAKEGYSYIHIQGNVVSKVEPREVRKFISDFVDKRYPELIKLRNMLH
                     NSRKLSESSLSNLKEVELDFTDFDKHYQYFFFNNETWKVTADGIEHKKNHLSNFEACV
                     WKNEVLPYNVKRLENSFNITYDEQTKCYDIDVLNTDSKFFSYL"
BASE COUNT          994 a          463 c          476 g          801 t
ORIGIN      
        1 atattatata gtttcttatt atctatttgt tttagaacct cctcgatttt attttcatca
       61 atttctattt gtaaaatcac acgtgatttt tgattttgtt taggttctga ttttgtaaat
      121 ggttcgaaga aattatagtc taatacttga ctaattgtaa acaattgctt tacgtccagg
      181 gagtcttttt cgagaatctt acccatgttt tgtttagtgg tattaattcg tcttgatagt
      241 tcggattgac tgattttctt ttcattaaga acatctttaa tccattgacc gatgtgaatt
      301 ttttttgctt ccataatagt ttaatttata aatagttaaa atgattattt tgttttggag
      361 tatattattt gattactttt tgtaaaaaaa gtattgactt tgtaaactat ttgtttactt
      421 ttgtataaaa tttcaatttt gaaactaact acaaataaca tgacaaacga aaaacttaca
      481 aaagagcaaa agcacacaat gtttaaaaca tggtggcttg cggttcccaa cggtatggta
      541 cgcatatacc gggaccaaat aaaaaaacag tgtggtatta ctaccgtagt attttaccat
      601 tggtttatgg gtaacagcga aatacctacg cttgcattac ctatcataga aaaaattgca
      661 ggggttgaag tgtttaatga aaataaatag cagctatgct aacactttca tattccacca
      721 cagatatatt cccattcaaa atacgcgata gagtgttaat acaatggcaa ataattcatt
      781 ggactgaaaa taatacatat tagctatgtt agataaaccc ctacatcaca tattacaaga
      841 agtatctaca tacttcgagg taacccccaa ccaaataaaa ggtaaatcac gcaaacagcc
      901 tttgccagat gcacggcatt tgtttattgt agaggcatac aaacaaggat atagcattac
      961 gtttattact cgttatataa attgctcaca cgctatgtat aagcatgcaa agtataaatc
     1021 tacatgcgac aatacctttc aaaaaattct aaaaaactac tcaaataact gatacaacta
     1081 tgtttgtaaa acaagaggat atactatctg ctacacgtgg cggacttgat ataatattgc
     1141 actattaccc acaggcacaa aaagctctta caactgccaa aaagcaattt aaggtacgac
     1201 ccgaaaaaac agcaagtgca acaataaaac aacttaccga tggcaactat gttgttaccg
     1261 actttggagg cgaccaaacc ccacgaaacg gtatacaagt gtgtatgtac gaggaaaatt
     1321 gcagcttcaa acaagctatt gaaattcttg ccgggcgaca caatataaac ggtatgcaaa
     1381 aaatagaacg caaagccgat taccaaaaac gtagtgctac tgcagacgaa aacgaaaacg
     1441 attttacggt agatataaaa gatgccatga gtaaattcga actcgaaacg ctcggtaaac
     1501 atgttactgc agaagtttgc aaaaagtata agttttacgc actcaaaagt ttttcgcgtg
     1561 taaaaaaccg cgaagtacat acctggttga gtaccgatga ttaccctata tatatgatag
     1621 atgggggcga ctacaaaaaa atactacaac catttgccga aaaacagtac cggttccaat
     1681 ggcttggcga aaataaaccc aaagatgtac tttttggtat ggatcagttg cgtgatgcat
     1741 acaaaaaaca tgaagaattg gcatttgctc aatttaacga agaagaagaa aaccgcaaaa
     1801 tagaacgcat ggaatgtgct attatcatga gtggtgatag agatgctata aatatggcag
     1861 catatggtta ccatgtattg tggctcaata gcgaaactgc taaaataagt accgaacaga
     1921 ttaaggaaat aagcaaatac gtaaaaaaaa tatacaatgt gccggatata gatactaccg
     1981 gcaaacgcga gggtataaaa cttgcactta cctatattga tatatgcacc gtgtggcttc
     2041 ccgaattttt actgcagtta aaagattcga ggggctatgg acgtaaagac ttcaaagatt
     2101 gggtagatgc caacgaaaac caaagccgta ttgatataca acacaaaatt gataaaatgc
     2161 ttaatgctgc attgtgtgca aaattttggt ttgaatacga aaccgaaaaa ggattacaat
     2221 acgaatttag caacgtacac ggctattatt ttcttgcttg caatggtttt cacattatca
     2281 aagatttaaa cgcaaaagag gggtattcat acattcacat acaaggcaat gtagtaagca
     2341 aagtagaacc acgcgaagta cgtaaattta tatctgattt tgtggataaa cgatacccgg
     2401 aattaatcaa attgcgtaac atgctccaca actcacgcaa attgagtgaa agctcgctat
     2461 ctaaccttaa agaagttgag ttggatttta ccgattttga caaacactac caatattttt
     2521 tctttaataa cgaaacgtgg aaagttactg ccgatggtat tgaacacaaa aaaaatcatc
     2581 tatcaaattt tgaggcatgt gtgtggaaaa acgaagtatt gccatacaat gtaaaacggc
     2641 tcgaaaatag cttcaatata acgtatgatg aacaaaccaa atgttatgat atagatgtac
     2701 tcaataccga tagcaagttt ttttcgtatc ttat
//
LOCUS       DAOTOW010000265         2731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29022_length_2731_cov_3.002990, whole genome shotgun sequence.
ACCESSION   DAOTOW010000265 DAOTOW010000000
VERSION     DAOTOW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29022_length_2731_cov_3.002990"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="P5243_05640"
     CDS             complement(<1..1023)
                     /locus_tag="P5243_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS18964.1"
                     /translation="MKSIAKKLFRIGYIILVLLLGAGISIALTKVSNQSKGPISSLFD
                     NVNLLIQKTEENLIVKKRENPRQDRIPWFNTIKNDIVALRNPSKIMVGIHDNKSLQSF
                     EEILTIEDSLQVVFPLIHLYVAWGDKTEHNFPKSQAQAIRAMGSIPVITWEPWLTAFE
                     GENYLHLRKKEIRDAGGMKDVANGLYDNYIIQWAQDIKNFKTPIFIRFAHEMNDPNRY
                     PWGPQNNEPSDFVKAWKHVWNIFNSIGAKNVLWVWTPHLSYGYIKYFYPGDEFVDYIG
                     LDILNFGTIASWSKWYTFSEILDNHYKELEAYKKPIMIAELGSLVYGGDRPTWYKEAL
                     ATIPTKY"
     gene            complement(1037..1930)
                     /locus_tag="P5243_05645"
     CDS             complement(1037..1930)
                     /locus_tag="P5243_05645"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS18965.1"
                     /translation="MDNLKNKRILVTGGNGYLGSFLVDALKSLGCEIFILDQKQVGNS
                     NEFGVDITIRNEVYNLLTKIKPHIVFHLAALLERGRDFSVHEKSLNVNYIGTMNLLLA
                     LKDIPEFEHFIFTSTSEVYGTNPSPFTEDMPPKPVSPYSMTKVFAEVAIQSVALNYGL
                     NYTILRLFNFFGENMPPNFFIPQMLNAFKYEEVFKMTNGEQARDFLYVGDIINALLLT
                     ATNPKSKQQIFNVCSAKSITLKDFVTLCHKSLQSTCRIDFGALPYRENEIWNMVGDNT
                     KIRQTLGFEVQYDISTIIQKI"
     gene            complement(1918..>2731)
                     /gene="rfbG"
                     /locus_tag="P5243_05650"
     CDS             complement(1918..>2731)
                     /gene="rfbG"
                     /locus_tag="P5243_05650"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HRS18966.1"
                     /translation="RDSYDDPKKTFDTNIGGTVNILEAVRLSSSVKAALVITSDKCYE
                     NKEWVWGYRENDPMGGHDPYSASKGGAEIVCSAYRRSFFDKAGCGPHIGFATVRAGNV
                     IGGGDWAADRLIPDIVRATSKKNSVYIRNPLATRPWQHVLEPISGYLTLGQRLLEKRV
                     DYADGWNFGPSPESNLTVDEVVQLCKKYWNNIAVEYSKNPADAHEANLLMLDCSKANK
                     IMHWKPVWNFDKIIEKTIEWYKNYYEANHINTRQDIKEYVQNAIENKVIWTI"
BASE COUNT          788 a          520 c          490 g          933 t
ORIGIN      
        1 atattttgtt ggtatagttg caagtgcttc tttgtaccat gtcggtctat cgcctccata
       61 tactaaagac cctaattcgg ctatcataat tggttttttg tatgcttcta attctttata
      121 atgattgtct aaaatttcag aaaatgtata ccattttgac catgaggcta tagtgccaaa
      181 attaagaata tcgagaccaa tataatcgac aaactcatcg cccggataaa aatatttaat
      241 atatccatag cttaaatgag gtgtccaaac ccaaagtaca ttttttgctc caatagaatt
      301 gaatatattc caaacatgtt tccatgcttt tacaaaatct gaaggttcgt tattttgtgg
      361 tccccaggga tatcggttag gatcattcat ttcgtgtgca aaacgaataa atataggtgt
      421 tttaaaattt ttaatatctt gtgcccactg gattatgtaa ttatcgtata gcccattagc
      481 aacgtctttc atccctccgg catcacgtat ttcttttttt cgtaaatgga gatagttttc
      541 tccttcaaat gcagttagcc atggttccca tgttattacg ggtatagagc ccattgctcg
      601 aattgcctgt gcttgacttt tggggaaatt atgttcggtt ttatcacccc aagctacata
      661 taaatgtatt aagggaaaaa caacctgaag agaatcttca atagttaaaa tttcttcgaa
      721 cgattgtaaa gatttattgt catgaatacc caccattatt tttgaaggat tacgaagggc
      781 aacaatatcg ttttttatag tattgaacca cggtattcta tcttgacgtg ggttttcacg
      841 ttttttaaca attagatttt cttcggtctt ttgaataagt aaattaacgt tatcgaatag
      901 agaacttatt ggaccttttg attgattact tactttggta agtgcaatcg atatgccggc
      961 acctaatagt aaaacgagga taatgtatcc tatccgaaat aacttttttg ctatgctttt
     1021 catacgtatt tgtgcattaa attttttgta taattgtgga aatatcatat tgaacttcaa
     1081 atcctaatgt ttgccgaatt ttagtgttat cgcccaccat gttccagatt tcgttttcac
     1141 gatacggtaa tgccccaaaa tcaatccgac acgtactttg caaactcttg tggcataaag
     1201 taacaaaatc tttcaatgtt atactttttg cactacatac attaaaaatt tgttgctttg
     1261 atttaggatt tgttgcagtt aaaagtaacg cattgataat atcgccaaca tataaaaaat
     1321 cgcgagcttg ctcgccatta gtcattttga atacctcttc gtacttaaaa gcattaagca
     1381 tttgtgggat gaaaaaattc ggaggcatat tttcaccaaa aaaattaaat aatcgtaaaa
     1441 ttgtataatt aagcccataa ttaagagcaa cagactgtat tgctacttcg gcaaatactt
     1501 ttgtcattga atagggcgat accggtttgg gaggcatatc ttcggtaaat ggtgaaggat
     1561 ttgtgccata aacctcgctt gtactcgtaa atataaaatg ttcaaattcg ggaatatctt
     1621 tcaatgcaag tagtaggttc attgtaccga tgtaattgac atttagagac ttttcgtgta
     1681 cactaaaatc tctcccccgc tccaacagag ccgcaagatg aaatacaata tgaggtttta
     1741 tttttgttaa aagattatat acctcattgc gaatggtgat gtctactcca aactcattac
     1801 tatttcctac ttgtttttgg tcgagaataa aaatttcaca tccgaggctt tttaaagcat
     1861 caactaaaaa agagcccagg tatccgttcc cgcctgttac aagtattcgt ttgtttttta
     1921 gattgtccat ataactttat tttcaatagc attttgtaca tattctttta tgtcttgtcg
     1981 ggtatttata tggtttgctt cgtaatagtt tttataccat tcaatagttt tttctattat
     2041 tttatcgaag ttccaaaccg gcttccaatg cataatttta tttgctttgg agcagtcgag
     2101 catcaaaagg tttgcttcat gagcatcggc aggatttttc gagtattcta ccgcgatgtt
     2161 attccagtac tttttgcata attgtacaac ctcatcaaca gtcagattac tttcgggaga
     2221 tggtccaaaa ttccacccat ctgcatagtc gacacgtttt tcaagtaatc gttgcccgag
     2281 ggttaggtat ccgctaatag gctctaatac atgttgccaa ggtctagttg ctaaaggatt
     2341 tcgaatataa actgaatttt ttttagaagt tgcacgaaca atatcaggga taagcctatc
     2401 ggcagcccaa tcgcctcctc cgatgacatt cccggcgcga acagtggcaa aacctatgtg
     2461 ggggccgcac cccgctttat caaagaaaga acggcgataa gcggagcaca caatctcggc
     2521 tccgcccttg cttgcgctgt aagggtcgtg gccgcccatc ggatcgtttt cacggtaacc
     2581 ccacacccat tctttgtttt cgtagcactt gtctgaggtg atgaccaatg ccgccttgac
     2641 ggatgaagac aaacgtacag cttcgagtat attgactgtt ccgcctatat tggtgtcaaa
     2701 ggtctttttg ggatcgtcgt atgaatcccg a
//
LOCUS       DAOTOW010000266         2730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29035_length_2730_cov_3.251963, whole genome shotgun sequence.
ACCESSION   DAOTOW010000266 DAOTOW010000000
VERSION     DAOTOW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29035_length_2730_cov_3.251963"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="P5243_05655"
     CDS             complement(<1..1845)
                     /locus_tag="P5243_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS18967.1"
                     /translation="MKHSILKLLVFSILFPGYIFAQSVSEFIAVDQFGYLPNSRKVAF
                     IRDPQVGYDAAKAFTPGNTYALVNNSTKQQVFTGTPQVWNGGATDASSGDKVWWFDFT
                     AYSTQGEYYVLDVQRNVKSYTFKIQENIYKDVLIQAFKTFYYQRFGFAKQAPYAATGW
                     IDGASHMGNLQDSQCRQWGKATDASTQRDVSGGWYDAGDMNKYTSWTAGYIMTMFTMY
                     EENKLAWTDDFQIPESGNGIPDILDEAKYGLDHLLRLQFTNGSCIAVVGCQSASPPSS
                     ATGQSLWGGPSTAATLACAAAYAYGAKIFKALGNTTYANTLQAAAIKAWDWAVANPSV
                     VFYNNDSNYGTSGLAAGQQETDDYGRLCKKLNAAAHLFEITGDTKYKTFFESNYLNVN
                     MMQWWYAFPYQAENQDILLYYTTIPNITASVKSEILNRYSVAMGRDNQFGVIDAKTDP
                     YQAHIESYTWGSNNVHMLQGHMFYSALMYNTNPERNAQIPHISEAYIHYIHGANPLNK
                     CYLSNMSAWGAENSVTEFFHSWFKDKSTKWDNTLTSTYGPAPGFLVGGPNPSYDWDGC
                     CPGQTCGSTQNNALCYEMSIEPPKNQPAQKSYKDFNSGWPLNSWSVTEN"
     gene            complement(1941..>2730)
                     /locus_tag="P5243_05660"
     CDS             complement(1941..>2730)
                     /locus_tag="P5243_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRS18968.1"
                     /translation="ISMKSVGIHNIGVYFFTPNTYNSNIEDICFYNNFSIDSTKTLYS
                     LSLNDFTIPNWWFDNAKLSPHESIPLDVSNIRYINISNAFTTQVNIVNSFVIYSVTFT
                     RNNTQLLLLLIGIFLIVCIVSCVIHFLYIKTSVPQSIIVTYKSVETPEETNKSHKVFE
                     FINSNYTDSELTLEHIAKHTGISSRKITSIIQETYQCNYKSYINTIRIEESKRLLSQT
                     QLQIGEIADAVGFSSQSYFNVVFKNIVGESPLVYREKNSSRNIQ"
BASE COUNT          874 a          491 c          430 g          935 t
ORIGIN      
        1 attttcggta accgaccaac tattcaaagg ccatccgcta ttgaaatctt tatacgattt
       61 ttgagcaggt tgatttttag gcggttctat agacatttca taacaaagag cattgttctg
      121 tgtgctaccg catgtttgac cgggacaaca accatcccaa tcataactcg gatttggtcc
      181 acctaccaga aaacccggag caggtccata cgttgatgta agagtattat cccatttggt
      241 acttttatct ttaaaccatg aatgaaaaaa ttctgttaca ctattttcgg ctccccaagc
      301 cgacatattt gataaatagc atttattcaa tggatttgct ccatgtatat aatgtatata
      361 tgcttcactt atatgcggta tttgagcatt tcgttcagga tttgtattat acatcaatgc
      421 actgtaaaac atatgtcctt gcaacatgtg cacattatta ctcccccatg tatatgattc
      481 tatatgagcc tgataaggat ctgtttttgc atcaattaca ccaaattgat tatcccgtcc
      541 catagcaaca gaatatctgt tgagtatttc tgattttacc gaagccgtaa tatttggaat
      601 tgtagtataa tataatagta tatcttgatt ttcagcttga tacggaaatg cataccacca
      661 ttgcatcata ttaacattta aataatttga ttcaaagaat gttttatatt ttgtatcgcc
      721 cgtaatttca aataaatgag ctgctgcatt taatttttta cataatctgc cataatcatc
      781 tgtttcttgt tgacctgcag ctaacccgga agtaccataa ttactatcat tattataaaa
      841 aactaccgac ggattggcaa ctgcccaatc ccaagcttta atggcagcag cttgcaacgt
      901 attggcatag gtggtatttc ctaaggcttt aaaaattttt gctccatagg cgtatgctgc
      961 tgcgcaagcc aatgtagcag cagtacttgg tcctccccat aaactttgac ctgtagcaga
     1021 cgatggagga gaagcacttt gacaacccac aacagctata cacgatccgt ttgtaaattg
     1081 taaacgcaat agatgatcaa gcccatattt agcttcatca agtatatcgg gtataccatt
     1141 acccgattca ggaatttgaa aatcatcggt ccatgccaac ttattttctt cgtacatggt
     1201 aaacatagtc ataatatatc ctgcagtcca actggtatat ttattcatat cacctgcatc
     1261 gtaccacccg ccacttacat ctctttgtgt gcttgcatca gttgctttgc cccattgccg
     1321 acattgggaa tcttgtaaat tccccatatg actggctcca tctatccagc cggttgctgc
     1381 atacggggct tgttttgcaa agccaaatcg ttgataataa aatgttttaa atgcttgaat
     1441 taaaacatct ttatatatat tttcttgtat tttaaatgta tatgatttaa catttcgctg
     1501 aacatcaagc acataatatt caccctgagt actatacgca gtaaaatcga accaccatac
     1561 tttatcgccc gatgaagcat cggtggctcc tccattccat acttgcggtg tgccggtaaa
     1621 cacttgttgt tttgttgaat tatttacaag agcgtaggta ttgcccggag taaacgcttt
     1681 tgcagcatcg taccctacct gagggtcgcg tataaacgct acttttcttg aattgggcaa
     1741 atatccaaat tggtcaacag caataaattc tgatacagat tgtgcaaaaa tataacctgg
     1801 aaataatatg gagaaaacta aaagtttcaa tatagagtgt ttcatagtga tatattttaa
     1861 aaaaaacagt atcacaaaaa tacatctctt acgcaactca tctttttcaa ttatttacaa
     1921 ttctttttaa aaatttgtat ttattgtata tttcttgagg aatttttttc acgatacaca
     1981 agaggacttt ctccaactat atttttaaat actacattaa aataactttg tgacgaaaat
     2041 ccaacagcat cggcaatttc acctatttgt aattgcgttt gtgacaataa ccgttttgat
     2101 tcttctatgc gaattgtatt aatatatgat ttataattgc attgatatgt ttcttgaatt
     2161 attgatgtaa tttttcgaga agatataccc gtatgctttg ctatgtgttc aagtgtaagt
     2221 tcactatcgg tataattact atttataaat tcaaatactt tatgtgattt attggtttct
     2281 tcaggtgttt caactgattt gtaggtaaca ataattgatt ggggtacaga tgtttttata
     2341 tacaaaaaat gaattacaca cgaaacgata cacacaatta aaaatatacc tataagtaaa
     2401 agcaataatt gtgtattgtt tcttgtaaat gtaacagaat aaattacaaa agaattaaca
     2461 atattaactt gagtagtaaa tgcattgcta atatttatat aacgaatatt tgatacatct
     2521 aaaggaattg attcatgagg cgaaagtttt gcattatcaa accaccaatt tggaattgta
     2581 aaatcattca atgaaagaga atatagtgtt ttggttgaat caattgaaaa attattataa
     2641 aaacatatgt cttcaatatt ggaattatat gtgttagggg taaaaaaata aacaccaatg
     2701 ttatgaattc ccactgattt catactaatt
//
LOCUS       DAOTOW010000267         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29063_length_2727_cov_3.050150, whole genome shotgun sequence.
ACCESSION   DAOTOW010000267 DAOTOW010000000
VERSION     DAOTOW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29063_length_2727_cov_3.050150"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1224)
                     /locus_tag="P5243_05665"
     CDS             complement(40..1224)
                     /locus_tag="P5243_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS18969.1"
                     /translation="MYTLARYSKYSIATIQVLYFLLYAAFASWSSYFYVFLENERHLT
                     GVQIGSIAAVQQINNIIFLPLWGMISDRYGKRNVFLLLLGISVFTLYGFLVHGGFVYY
                     LVFIVFFSALHNPIGSLIDSFALRKAKEPLLTTSYGKMRLWASVGWAVSSVVTGFVIK
                     YTSISYIFPIASALLAISWVISFLYVNKKRETTTDVKPSYRALFVFLQTNKQLLWFFL
                     FIMLYYIFNSPTLMFINLYYNEIGATNSDIGIAFAVQSVFELPFLFFGNTIIKKYGVK
                     NVILFTMFVAAVRMVLYGFTSNPWIAITIGCLHGVTLGLFLVAAIEYTHRLVPIQFNS
                     TGQTLFYTFLGVGTSVGNLLNGFLKDYMSLQHAMKVNASVVFVMVIVSYVFLQKKSKQ
                     IV"
     gene            complement(1259..1828)
                     /gene="gmk"
                     /locus_tag="P5243_05670"
     CDS             complement(1259..1828)
                     /gene="gmk"
                     /locus_tag="P5243_05670"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRS18970.1"
                     /translation="MTHQKMIIFSAPSGSGKSTIINFLLQKGYPIEFSISATTRSPRG
                     TEKHGVEYYFLSLKEFKQKIERDEFLEYEEVYENRFYGTLRSECDRIWNQGKIVVFDV
                     DVAGGIRLKELFGDKALSIFIQLPSIEELRKRLENRKTDTPEEIEKRITKASFEIQFS
                     YMFDTIVVNDNLEQACNEVEKLVSEFIAK"
     gene            complement(1833..2705)
                     /locus_tag="P5243_05675"
     CDS             complement(1833..2705)
                     /locus_tag="P5243_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRS18971.1"
                     /translation="MIQSMTGYGKAVAEWTDKNIVIEIKSLNSKQLDINARISHIFRE
                     KELEIRSIISDKLVRGKIDIALYVEYKEGVSEKTINTALVSSYCNQLRAIALQQQLPE
                     HGILDIAMRMPDVLISEPVAELNDAMWELVKQALTQAVDSITAYRINEGKALAHDILH
                     NIEAIGNLMQQIAPFELGRLQKIKDRIQNSLDEAVGLDKIDANRLEQELIFYVEKLDI
                     NEEKVRLLQHCEYFKVTMNEPQCGKKLSFIAQEMGREINTIGSKSNDADMQKIVVHMK
                     DYLERIKEQVLNIL"
BASE COUNT          944 a          507 c          354 g          922 t
ORIGIN      
        1 ttacaaaaaa aaatactttt tttataaaaa aagacaaaat tatactatct gttttgattt
       61 tttttgaaga aacacatacg aaactataac cataacaaac actactgatg catttacttt
      121 cattgcatgt tgtaagctca tgtaatcctt caaaaaacca ttcaatagat tacctacact
      181 agtacctact cctaaaaacg tatagaataa tgtttgacct gtagaattaa attgtatagg
      241 tactaaccta tgagtatatt ctatagctgc taccaaaaac aatcctaaag taacaccatg
      301 caaacaacca atagtaattg caatccacgg atttgatgta aaaccgtata aaaccatacg
      361 aacagctgca acaaacatgg taaataaaat cacattttta actccatact ttttaataat
      421 agtatttcca aaaaataaaa atggtaattc aaataccgat tgaaccgcaa atgcaattcc
      481 tatatcagaa tttgtagccc ctatttcatt gtaatacaaa ttaataaaca taagtgtagg
      541 cgaattaaaa atataataca gcataataaa caaaaagaac cacaacaatt gtttattggt
      601 ttgcaaaaac acaaacaaag ctctgtaact tggttttaca tcggtagttg tttcgcgttt
      661 tttattcaca tacaaaaacg aaataaccca cgaaatagcc aatagtgctg atgctatagg
      721 aaaaatataa gaaattgacg tatatttaat aacaaaaccg gtaactacag aagaaacagc
      781 ccatcctacc gatgcccaca atcgcatttt gccatatgat gttgtaagca agggttcttt
      841 agctttgcgc aatgcaaatg aatcaataag tgaacctatg ggattatgta aagcagaaaa
      901 aaatacaata aatactaaat aatacacaaa tccaccatgt actaagaaac catacaatgt
      961 aaatacagaa atacctaata acaataaaaa tacatttcgt ttaccgtacc tatccgatat
     1021 cattccccac aatggaagaa atataatatt attgatttgt tgtacagcgg ctatgcttcc
     1081 tatttgaaca cccgtaagat gacgttcgtt ttctaaaaac acataaaaat acgatgacca
     1141 tgaagcaaac gcagcgtaca ataaaaaata taagacttga attgttgcaa ttgaatattt
     1201 agaatatctt gcaagagtat acatgatttc tccaatttat ttttttgagc acactcattt
     1261 actttgcaat aaattcagaa actaacttct ctacttcatt gcatgcttgt tccaagttat
     1321 cattcactac aattgtatca aacatatatg aaaattgtat ttcaaaactt gctttggtaa
     1381 ttcgtttttc aatttcttcg ggagtatctg tttttctgtt ttctaatcgt ttacgtaatt
     1441 cttctatcga tggtaattgt ataaaaattg acaaagcctt atcaccaaac aattctttga
     1501 ggcgaatacc tcctgctaca tctacatcaa atactacaat ttttccttga ttccaaattc
     1561 ggtcacattc agaacgcaag gtgccataaa agcgattttc atacacttct tcatactcca
     1621 gaaattcatc acgttcaatt ttttgtttaa actccttgag cgacaaaaaa taatactcta
     1681 caccgtgttt ttctgttcct cgcggactcc gtgtagttgc cgaaattgaa aattctatag
     1741 gatatccttt ttgtaataaa aaattaataa tagtagattt tccgctaccc gatggtgccg
     1801 aaaatattat cattttttga tgtgtcatat acttataaaa tatttaaaac ctgttcttta
     1861 attctttcca aataatcttt catgtgaaca acaattttct gcatatcggc atcatttgat
     1921 tttgaaccta ttgtatttat ttcgcgtccc atttcttgtg ctatgaaact taatttttta
     1981 ccacattgag gttcattcat ggtaacttta aagtattcac aatgctggag aagtcgcact
     2041 ttttcttcgt ttatatcaag tttttccaca taaaaaataa gttcttgctc cagtcggttt
     2101 gcatctattt tgtctaaacc aacagcttcg tccaaagagt tttgtatacg gtctttaatt
     2161 ttttgcaaac gacccaattc aaacggagct atttgttgca ttaaattacc aatagcttca
     2221 atattatgta atatatcatg tgccaacgct ttaccttcgt ttattctata agccgtaata
     2281 ctatcaacag cttgcgttaa agcttgtttt acaagttccc acatagcatc attcaattct
     2341 gctaccggtt cagaaataag tacatcgggc atgcgcatag ctatatctaa aataccatgt
     2401 tcgggcaatt gttgttgtaa agctatagca cgcaactgat tacagtatga tgataccaaa
     2461 gcagtattaa ttgttttttc gcttacacct tctttgtatt ctacatacag agcaatatca
     2521 attttacctc gtaccaattt gtctgatata atagaacgta tttcgagttc tttttcacga
     2581 aaaatatgag aaattctcgc atttatatcg agttgtttac tgttcaacga ttttatttct
     2641 attacaatat ttttatcggt ccattctgct acagctttac catagccggt cattgattgg
     2701 atcatactta cttatttttt agaatac
//
LOCUS       DAOTOW010000268         2721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29140_length_2721_cov_3.499250, whole genome shotgun sequence.
ACCESSION   DAOTOW010000268 DAOTOW010000000
VERSION     DAOTOW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29140_length_2721_cov_3.499250"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..>2721)
                     /locus_tag="P5243_05680"
     CDS             complement(37..>2721)
                     /locus_tag="P5243_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRS18972.1"
                     /translation="AQESIELGNKIKDLHTEIPIYVLLNQRSNVAYFQQLILQTSLFN
                     KIFIWNGDSQIFFAMVKSLEDLANVENDTKIGLVRAILLIEDSPYYYSKYLPAIYSVF
                     FNQVGEQIIDYEMNELEKISRMRSRPKLLWATNFEQASFLYNKYKDFITCIICDGEFE
                     QFGTIQKHSGRQFLEKIREVDFNIPILIQSSDDAFRLLAKKIKATFIHKHSEVLLNAL
                     KRFVMHNFGYGDFIFRNSKGKPIAKARNLREFETLLRIIPDESLKFHAQQNQFSIWLM
                     GRGEIGLARQINPIQLQDFKTIDELRKKNIEIFEEHKRTKKRGKILSFEETVEIDETS
                     IVSLCGGSLGGKGRGIAFANALIQNVDFTEFSNRIIISTPKTAIVGTDEFEHFIKRNY
                     LDKQLFKKEYTDDEIKRMFFEGVLSAELRKKLLALLEQIIKPIAVRSSSIFEDSLNQP
                     LAGLFSTYILPNNGSIDKRLEDLEVAIKLVFASVFLKHVKSFYKNTYLKIEEEKMAVV
                     IQELVGNYYDTYFYPHISGVAQSYNYYPVSYMKPEEGVAVCAIGLGYYIVDGGKSYRF
                     SPVYPKLQMLSIKNLIQSTQTQFLAVDLQKQTYNFLLEGERSALSLLPISKAEQHNTL
                     THCVSIYNPHNDRLEPGLSSVGIRVVNFANILEYDYIPLSKILTRMLEVVKEAMGTAV
                     EIEFAIDLTKNEQGLPTFYLLQIKPVSNNYNFEIIDESIFESNNILLQSSQALGNGIE
                     QTIFDVVYIKPESFDKMRTIEMVQEIEEINKKMIAQNKQYILIGPGRWGTSDRFLGVP
                     VQWPQISNAKVIVETSLSDFPLNSSLGSHFFHNITSMNVGYIAIEQKNNQNSIEWEIL
                     EKQTCVEETVFYKHISFKNNLIVHMNGKQRNAIIYYKE"
BASE COUNT          904 a          450 c          332 g         1035 t
ORIGIN      
        1 gatgaagacc tactattgat atacgaatat tactcattat tctttataat atataattgc
       61 atttctctgt tttccattca tatggacaat taagttattc ttaaaagaaa tatgtttata
      121 aaaaactgtt tcttctacac atgtttgttt ttctaaaatt tcccattcaa tactattttg
      181 attatttttt tgttcaattg caatatatcc cacattcata gatgttatgt tatgaaaaaa
      241 atgagaacca agagatgaat ttaatgggaa atcagaaaga cttgtttcaa caataacttt
      301 tgcattcgaa atttgaggcc attgaaccgg tactcccaaa aatctatcgc tagtacccca
      361 cctacccggt ccaattaaaa tatattgttt attttgcgct atcatttttt tatttatctc
      421 ctctatttct tgaaccattt ctatagtgcg cattttatca aatgattccg gttttatata
      481 tactacatca aaaatagttt gttcaatacc atttcccaat gcttgggaac tttgtaataa
      541 tatattattt gattcaaaaa tcgactcatc aattatttca aaattataat tatttgatac
      601 tggttttatt tgtaataaat agaatgttgg taatccttgc tcatttttag ttaaatcaat
      661 agcaaattct atttcaacgg cagttcccat tgcctctttt acaacctcaa gcattcttgt
      721 aagtattttg cttaatggta tataatcgta ctctaatata tttgcaaaat ttacaacacg
      781 tatacctacc gatgataatc cgggttcaag tctatcatta tgtggattat aaatggaaac
      841 acaatgagtt aacgtgttat gttgttctgc ttttgatata ggcaacaagc ttaatgcaga
      901 acgctctccc tctagtaaaa aattatatgt ttgtttttgt aaatcaactg ctaaaaattg
      961 tgtttgagta gattgaatta gatttttaat tgaaagcatc tgtaattttg gatatacagg
     1021 agaaaatcta tatgattttc ctccatcaac tatataatat cctaatccaa ttgcacaaac
     1081 agcaactcct tcttcgggct tcatataaga aacaggataa taattatatg actgggcaac
     1141 tccacttata tgtggataaa aataagtatc atagtaattt cctactaatt cttgaataac
     1201 cacagccatt ttttcttctt ctatttttaa atatgtattt ttataaaatg attttacatg
     1261 ttttaaaaat accgaggcaa atactaattt aattgcaacc tctaaatcct ctaatcgttt
     1321 atctatacta ccattattgg gtaaaatata tgtactgaat aatcctgcta agggttgatt
     1381 caaagagtct tcaaaaatac tcgaagaacg aactgctata ggttttataa tttgttctaa
     1441 cagagcaagc aattttttgc gtaactcagc agacaataca ccttcaaaaa acatccgttt
     1501 tatttcatcg tctgtgtatt cttttttgaa taattgttta tctaaataat tacgttttat
     1561 aaaatgttca aattcatctg ttccaacaat agctgttttg ggagttgaaa taataattct
     1621 attggaaaat tctgtaaaat ctacattttg aatcaatgca tttgcaaatg caattcctct
     1681 acctttgcca cctaatgaac ctccacataa actaactata cttgtttcat ctatttcaac
     1741 agtttcttca aaactaagaa tcttgccacg tttttttgtt cttttatgtt cctcaaaaat
     1801 ttcaatattt ttttttctca attcatcaat agttttaaaa tcttgcaatt gaatagggtt
     1861 tatctgacgt gccaacccta tttctccacg ccccattaac caaatagaaa attggttttg
     1921 ttgtgcatga aacttcaatg attcgtcagg tattatgcgc aacaatgttt caaattcacg
     1981 taaatttctt gctttagcta ttggttttcc tttcgaattt ctgaatataa aatcaccata
     2041 gccaaaatta tgcataacaa accgtttaag tgcatttagc aatacttcgg aatgtttatg
     2101 aataaatgta gcttttattt tttttgctaa taatcgaaaa gcatcatcgc ttgactgtat
     2161 taaaatagga atattgaaat caacttcacg aattttttct aagaattgtc ttccagaatg
     2221 cttttgtatt gtaccaaatt gttcaaattc tccatcacat ataatacagg ttataaaatc
     2281 tttatattta ttatataaaa aacttgcctg ctcaaaatta gttgcccaca ataatttggg
     2341 tcgagaacgc atccttgaaa ttttttcaag ctcattcatt tcataatcaa ttatttgttc
     2401 tcctacttga ttaaaaaaga cagaatatat tgcaggtaaa tattttgaat aataatatgg
     2461 agaatcctct ataagtaaaa ttgctctcac caacccaatt tttgtatcat tttcaacatt
     2521 tgccaaatct tctagcgatt taaccattgc aaaaaatatt tgagaatctc cattccaaat
     2581 aaaaatttta ttaaaaagtg aggtctgtaa tattaattgt tgaaaataag caacatttga
     2641 cctctgattt aataatacat atattggaat ttctgtatgc aaatctttga ttttgtttcc
     2701 taactcaata gattcttgag c
//
LOCUS       DAOTOW010000269         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29190_length_2717_cov_2.678062, whole genome shotgun sequence.
ACCESSION   DAOTOW010000269 DAOTOW010000000
VERSION     DAOTOW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29190_length_2717_cov_2.678062"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="P5243_05685"
     CDS             complement(<1..231)
                     /locus_tag="P5243_05685"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase"
                     /protein_id="HRS18973.1"
                     /translation="MNIYHSLKDFSATNAVVTIGMFDGVHVGHRALLQHVMLEASVIN
                     GESVVLTFWPHPQVFFGKTHDFKMLSTLDEKLS"
     gene            complement(248..2038)
                     /gene="lepA"
                     /locus_tag="P5243_05690"
     CDS             complement(248..2038)
                     /gene="lepA"
                     /locus_tag="P5243_05690"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRS18974.1"
                     /translation="MKYIRNFCIIAHIDHGKSTLADRLLEVTNTISERDAQEQVLDNM
                     DLERERGITIKSHAIQMDYVYKGETYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISNLYLAIEHDLEIIPVLNKMDLPSAMPEEVKDQIVELIGCKREDILEA
                     SGKTGLGVDKILETIVEKVPSPKGSPDAPLQALIFDSVFNSFRGIIAYFKVVNGVIRK
                     GDKVKFVNTKKEYEADEVGILRLDMIPRDMVGTGDVGYIISGIKTSKEVKVGDTITHT
                     LNPCEKAISGFEDVKPMVFAGIYPIDTEDYEELRASMEKLQLNDASLTYEPESSLALG
                     FGFRCGFLGLLHMEIIQERLDREFDMSVITTTPNVSYVCYTKKQERIVVNNPTDLPDP
                     SKIDYIEEPYIHAQIITNAEYIGPIMSLCIGKRGILKSQTYMTGNRVEVTFEMPLGEI
                     VFDFYDKLKSISKGYASFDYHVIGYRESKLAKLDILLNGEPVDALSALIHTSNAYEFG
                     KKICEKLKELIPRQQFDVAIQAAIGSKVIARETVKAVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMKQVGNVEVPQKAFLAVLKLND"
     gene            2260..2565
                     /locus_tag="P5243_05695"
     CDS             2260..2565
                     /locus_tag="P5243_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18975.1"
                     /translation="MKKNLFFLLYLFLSIISISSIAYSQTVPTDSLFSISFDELMNMK
                     VSSGSKIVQRANEVNAKVKIIKCDDIQKRGYNTIDEVLQDLPGFQSRNIQSINSYIF"
BASE COUNT          908 a          503 c          381 g          925 t
ORIGIN      
        1 cgataatttt tcatctaagg ttgataacat tttaaaatca tgagttttac caaaaaacac
       61 ctgagggtgt ggccaaaaag taagaacaac agattctccg ttaattaccg atgcttctaa
      121 cattacatgt tgtaacaatg ccctatggcc aacatgcaca ccatcgaaca tacctatagt
      181 tacaacagca ttagtagcag aaaaatcttt aagagaatga taaatattca ttgtttgaaa
      241 aaggttttta atcattcagt tttagtactg ccaaaaatgc tttttgaggc acttctacat
      301 taccaacttg cttcatacgt tttttacctt ttttctgttt ttcaagcaat tttcgtttac
      361 gagaaatatc accaccataa cattttgcag taacatcttt acgaacagct tttaccgttt
      421 cacgagcaat aactttactc cctattgctg cttgaatagc aacatcaaac tgttgtcgag
      481 gaattaactc ttttaatttt tcacagattt ttttaccaaa ctcatacgca ttactagtat
      541 gaattaaagc agaaagagca tctacaggtt caccattcaa caaaatatcg agttttgcta
      601 attttgattc tctataacca attacatgat aatcaaacga tgcatatccc tttgaaatac
      661 ttttcagttt atcataaaaa tcaaaaacta tttcacctaa aggcatttca aaggttactt
      721 ctaccctatt accggtcata tatgtttgac tctttaaaat accacgtttg cctatacaca
      781 aactcataat aggaccaata tattcggcat ttgtaataat ttgggcatga atatacggtt
      841 cttctatata atcaattttt gaagggtcgg gtaaatcggt ggggttattt acaactattc
      901 gttcttgttt ttttgtataa catacatacg acacatttgg agtagttgta attacactca
      961 tatcaaactc tctgtccaag cgttcttgaa taatttccat gtgtaacaat ccaagaaatc
     1021 cacaacgaaa tccaaaacca agagccaatg aagattccgg ttcataggtt aatgaagcat
     1081 catttaattg caatttttcc attgaagcac gcaattcttc ataatcttcg gtatcaatgg
     1141 gataaatacc tgcaaaaacc atcggtttta catcttcaaa acctgaaatt gctttttcac
     1201 aaggatttag tgtatgtgtt atagtgtcac caacctttac ttcttttgaa gttttaatac
     1261 cggaaataat ataccccaca tctcctgttc ctaccatgtc acgaggaatc atatccaatc
     1321 tgagaatacc aacctcatcg gcctcgtatt cttttttagt atttacaaat tttactttat
     1381 caccttttcg tattacacca tttactactt taaaataggc aataattcct cgaaacgaat
     1441 taaaaaccga atcaaaaatc aatgcctgta acggagcatc gggactacct tttggcgatg
     1501 gtactttttc aacaattgtt tctaatattt tatctactcc caatcctgtt ttaccacttg
     1561 cttcaagaat atcttcacgt ttacaaccaa tcaattctac tatttggtct tttacttcct
     1621 cgggcatagc actgggtaaa tccattttat taagcaccgg aataatttcc aaatcatgct
     1681 caatagctaa atataaattt gaaattgttt gagcctgtat accttgggta gcatctacaa
     1741 taaggagagc accttcgcat gctgcaatag aacgagaaac ttcatacgaa aaatcaacat
     1801 gccccggagt atctattaaa ttcaatgtat atgtttcgcc tttatataca taatccattt
     1861 gaatagcatg actttttatg gtaataccgc gttcacgttc caaatccata ttatcgagca
     1921 cttgctcttg tgcatcacgt tctgatatgg tattggttac ttcaagcagc ctatcggcta
     1981 atgtactttt accatgatca atatgtgcta taatgcaaaa attacgaata tacttcatga
     2041 gttcttataa aattgtataa aaatttgcta ttcaactgtg tatatacaaa tatatgcact
     2101 ccatttagca gcatttgttt cttaagccgc aaatataata aaaaacattc gcaaatagta
     2161 cgcataaaaa aataggcatt tgaaagtata atttcttgtt tttttatata tttgtaacat
     2221 atagagtaga aacaagacag acattttatt acaaaacaca tgaaaaagaa tctttttttt
     2281 cttttatatc tttttcttag tataatcagt atatcctcta ttgcttattc tcagactgta
     2341 cctacagact ctttatttag tatttcattt gatgaattaa tgaatatgaa agtaagcagt
     2401 ggctcaaaaa tagtacaacg tgctaatgag gtaaatgcaa aagtaaaaat aataaaatgc
     2461 gatgatattc aaaaacgagg ctacaataca atagatgagg ttttacaaga cttgccggga
     2521 tttcaatcta gaaatattca aagtataaac agttatattt tttgacgagg aattcccaat
     2581 caaaataatt taatgcttct attaattgac ggaattcaaa taaacgaact caattcagga
     2641 ggtttttacg gtggcgctca gtacaattta gataatgttg agcgtattga aattgtatat
     2701 ggacctgctt cggtagt
//
LOCUS       DAOTOW010000270         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29330_length_2707_cov_3.213424, whole genome shotgun sequence.
ACCESSION   DAOTOW010000270 DAOTOW010000000
VERSION     DAOTOW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29330_length_2707_cov_3.213424"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="P5243_05700"
     CDS             <1..518
                     /locus_tag="P5243_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRS18976.1"
                     /translation="DLRTYDFCAIGEIGIDLYWDTTYTNQQIEAFEYQLFMAQKLHKP
                     VAIHVRNSWQQVLHSIHKFPDVIGVFHCFSGNIQQAYSVIDNGFKLGIGGVVTYKNSG
                     VDTVVEAIDLQHMVVETDDPWLAPVPYRGKVNEPSYISYIIQKIAVIKNCKIELVEEI
                     TSSNALQIFSL"
     gene            521..1762
                     /locus_tag="P5243_05705"
     CDS             521..1762
                     /locus_tag="P5243_05705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRS18977.1"
                     /translation="MSFASVYFSRFRVQEPIITEVPSSELGIIVTIPCHNEPDILETL
                     QSLAHCIPPPCKTEVIVVVNNSEKAPNEIVYINNVTYNNIVQYINHFNTEFIRLHCVY
                     VPNMSYKHAGVGFARKIAMDEATHRFAYTNNHKGIIASLDADCTVHQSYLLSIYQTFT
                     SYDCGIAPIYFEHPLSGNLSYYQYFAIAQYELYLRWYVYMLRSIGFPFAHHTVGSSFA
                     VRADAYCRQGGMNKRQAGEDFYFLQKMYEAEQVSSITNTTVYPSARVSHRVPFGTGYA
                     MQTLMNSADNIYYVYNPYSFYPLNDFFTRINELYKCNSERCIQFYKSLHYTIQLYVSL
                     EVFEAKILECNTHSRSKDFFVKRFFLWFNGFMIFKYLNFVHTKGFFEKISIQHAASQT
                     LELDSNVFELLKMYRKIDRNG"
     gene            complement(1767..2360)
                     /locus_tag="P5243_05710"
     CDS             complement(1767..2360)
                     /locus_tag="P5243_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRS18978.1"
                     /translation="MQKVKLNILGLSFSQTQDNAYVLILVEEEGERRIPIIIGGTEAQ
                     AIAIELENMKAPRPLTHDLFRSLAFEFEISLEEVIIYKLQEGIFYSKIVVKQNDKTVE
                     IDSRTSDAVALAVRFNSPIYTTSDVILRAGIVLDLERKHQEAQEKNESETSEEQNSLA
                     FESDDDLKHMLNQAIQEEDYEKASKIRDEIKRRSEMQ"
BASE COUNT          855 a          423 c          433 g          996 t
ORIGIN      
        1 aagatttgag gacctatgat ttttgtgcga taggtgaaat tggaatagat ttgtattggg
       61 atactacgta tactaatcaa caaatagaag catttgaata tcagttgttt atggctcaaa
      121 aattacataa gccggttgct attcatgtgc gaaattcatg gcaacaagta ttacatagta
      181 ttcacaagtt tcccgatgtt attggagttt ttcattgttt ttcgggtaat attcagcaag
      241 cttattcagt aattgataat ggttttaagt tggggatagg gggtgttgta acatataaaa
      301 attcaggagt tgacactgtt gttgaagcta ttgatttaca acatatggta gtagaaaccg
      361 acgatccttg gttagctcct gtaccatata gaggtaaagt taatgagcct tcgtatattt
      421 catatataat tcaaaaaata gcagttataa agaattgtaa aattgaatta gtagaagaaa
      481 ttacttcaag taatgcttta caaatttttt cactgtaatc atgtcgtttg catctgttta
      541 tttttctcgt tttcgtgttc aagaaccaat aattaccgaa gtcccctcat cggaattagg
      601 tattattgtt actatacctt gtcataatga acccgatata cttgaaacat tacaaagttt
      661 ggcacattgc attcctcctc cctgtaaaac agaagtgata gtagttgtaa ataacagtga
      721 aaaagcacct aatgaaatag tttatataaa taatgtaaca tacaataata tagtacaata
      781 tataaatcat tttaatacag aatttatacg tttacattgt gtgtatgttc ccaatatgtc
      841 ttataaacat gccggtgtag gttttgcgcg taaaattgct atggatgaag caactcatag
      901 atttgcatat accaataatc ataagggtat tattgcatca ttagatgcag attgcactgt
      961 acatcagtct tatttgttgt ctatttatca aacatttaca tcgtacgatt gtggtattgc
     1021 tccaatctat ttcgaacacc cgctatcagg taatttatca tattatcaat attttgcaat
     1081 tgctcaatat gagctttact tacgatggta tgtttatatg ctgcgctcta taggttttcc
     1141 ttttgctcat catactgtgg gatctagttt tgccgtacgt gccgatgctt attgcaggca
     1201 gggtggaatg aataaacgtc aagcaggtga agatttttat tttttacaaa aaatgtacga
     1261 agcagaacaa gtttcctcaa ttacaaatac tacagtatat ccatctgcgc gggtttctca
     1321 tcgtgttcct tttggaacag gctatgctat gcaaaccctt atgaatagtg ctgataatat
     1381 atattatgtg tacaatccct acagttttta cccattgaat gattttttta ctagaataaa
     1441 tgaactttat aaatgtaatt ctgaaagatg tatacagttc tataaatcat tacactatac
     1501 tatacaactg tatgtttcac ttgaagtttt tgaggctaaa attttagaat gtaatacaca
     1561 ttctcggtca aaagattttt ttgtaaaacg attttttttg tggtttaatg ggtttatgat
     1621 attcaaatat ctcaattttg tacatactaa aggatttttt gaaaaaattt ctattcaaca
     1681 tgctgcttca caaacactag agttggattc aaatgtgttt gaattgctta aaatgtatag
     1741 aaaaatagat agaaatggat aaatcattat tgcatttcgc ttcttcgttt aatttcatca
     1801 cgaattttag aagctttttc ataatcttct tcttgaattg cttgattgag catatgtttt
     1861 aaatcgtcat cagattcaaa agctaaacta ttttgttctt cagaagtttc agattcattt
     1921 ttttcttgag cttcttgatg ttttctttcc aaatctaata ctataccggc acgtaaaatt
     1981 acatcgctgg tagtataaat agggctattg aaacgtactg ccaatgcaac cgcatcggag
     2041 gttctcgaat ctatctcaac tgttttgtcg ttttgtttta caactatttt tgaataaaaa
     2101 ataccttctt gcaatttgta aattatgact tcttcgagcg agatttcgaa ttcaaaagct
     2161 aaactgcgaa acaagtcgtg agtaagcgga cgaggtgctt tcatgttttc taactctatg
     2221 gcaattgcct gagcttctgt accacctatt ataataggta tacgacgttc accttcttct
     2281 tcaactaaaa ttaatacata ggcattatct tgtgtttgac taaatgacaa tcccaaaata
     2341 tttaacttta ctttctgcat atctaaaatt tactgttcaa agatacatgt tttttgataa
     2401 aaatatctta ggacatgcaa attttgtagg gattacattt ttttcaactg aaaatacata
     2461 ttaataaaca ttcgtttcat acaagtatta aagttctgat tgtatcatca aaacacaaat
     2521 ttgaatatgt aacgagctac tttgaaaact cattttatct acaacgtctg caatataatc
     2581 cgctctattt caattttttt gtcggaactt atatcgcctt gactttgcac acattctaaa
     2641 agttttttct tgaaactcca gcaagtcatt tgtcgtaatt cgagtttttg agataattcg
     2701 tgcgatg
//
LOCUS       DAOTOW010000271         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29334_length_2707_cov_2.793363, whole genome shotgun sequence.
ACCESSION   DAOTOW010000271 DAOTOW010000000
VERSION     DAOTOW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29334_length_2707_cov_2.793363"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="P5243_05715"
     CDS             complement(<1..705)
                     /locus_tag="P5243_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HRS18979.1"
                     /translation="MALRFITAEEAASYINHNDNVAFSGFTPAGSPKAVPTAIAERAI
                     KEHEAGRPFKIGMFTGASTGDSLDGALARANAVKFRTPYQSNKDMKNLINTGQTEYFD
                     MHLSQVAQELRYKFLGDIDVAIIEAADITEKGEVVLTSAVGISPTAARLAKKVIIELN
                     ANHPAAIKGIHDIYELQDPPIRRAIPIYTVKDLCGSPVLQIDPAKIIGVVETNRPDEV
                     AGFSPVDEVTAKIGDNV"
     gene            complement(859..1284)
                     /locus_tag="P5243_05720"
     CDS             complement(859..1284)
                     /locus_tag="P5243_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18980.1"
                     /translation="MIQFERHRKRNKTNKNLIIITIGFLCSVLVAAFVFQTYISKLHE
                     TKKNNPKILSITIENNSMVKELPYFLIIQHDTILRKTVHLGKQNYQIAWKWDTVPVIQ
                     HILGTNYELRNVYVNDSVSYIMVEILGKNKVQIIQHFFE"
     gene            complement(1329..2021)
                     /gene="radC"
                     /locus_tag="P5243_05725"
     CDS             complement(1329..2021)
                     /gene="radC"
                     /locus_tag="P5243_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRS18981.1"
                     /translation="MNSNFLSIKSWAEEDRPREKLQLKGVHALSDAELLAILISTGTQ
                     NESALDIAKRIVHLSGNCLQELGRYSLHDLTKIKGIGPAKAITLLAAFELGKRRSATE
                     VKPTNPITSSNQVYRLLHPSMADLAHEEFRVLYLNRNNSIIAQKVISVGGVAGTVIDV
                     KIIGKAALEYLASGIIVAHNHPSGNTNPSNADKEITKKIQQAAPFFDCTMLDHVIVTR
                     NGYYSFADNGEL"
     gene            complement(2087..2497)
                     /locus_tag="P5243_05730"
     CDS             complement(2087..2497)
                     /locus_tag="P5243_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18982.1"
                     /translation="MKYIFSIVSLFICFGMYAQTGISTETPNAATVLHIVAPNQNTGV
                     LIPTLTSAQISAIASPTHSLLVYNTTKQKFMYNAGTSGTPLWTFVGDIPAINDISTLT
                     TGEAGDVRYNKANGQIYYWKVGFGWKQLQSIAGP"
     gene            complement(2509..>2707)
                     /locus_tag="P5243_05735"
     CDS             complement(2509..>2707)
                     /locus_tag="P5243_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18983.1"
                     /translation="TRNEFMYYNSLEWRPFSYIESVSTNSKVGMYEGETKLYTTGNVL
                     IFLNNANNWRQISIGAGTITP"
BASE COUNT          839 a          496 c          399 g          973 t
ORIGIN      
        1 tacattatca ccaattttag cagttacctc atctacggga ctaaatccag caacctcatc
       61 gggtctattt gtttctacta caccaattat ttttgcagga tctatctgta aaacgggaga
      121 accacacaaa tctttcacag tataaattgg tatagctcta cgaattggcg gatcttgtaa
      181 ttcataaata tcatgaattc ctttaattgc agccgggtga tttgcattta attctataat
      241 aacttttttt gctaatcgag cagctgtagg cgatattcca actgcagaag tcaatactac
      301 ttcacctttt tctgtaatat cagcagcttc tataatagca acatctatat cacccaaaaa
      361 tttataccgt aattcttgag caacttgaga aagatgcata tcaaaatact cggtttgccc
      421 tgtatttata aggtttttca tatctttatt tgactgatat ggcgttctaa attttaccgc
      481 atttgcacga gctaatgctc catcgagaga atctccggta gatgctccgg taaacatacc
      541 tattttaaaa ggtcgtcccg cttcatgttc ttttattgct cgttctgcta ttgctgttgg
      601 taccgctttg ggtgatccgg caggtgtaaa accactaaac gccacattgt cgttgtgatt
      661 aatgtaagaa gctgcttctt cagcagttat aaatcgtaat gccatatgtt tttatatttt
      721 ttttaagtaa atatttgcta acaaaaatag tatttgttgc gaaactgcac aaaactattc
      781 tataaaactt atcataaaaa attgattatt tttatcattt ttttagtgtt ctcaatattg
      841 tattcccatt agcttatttt actcaaaaaa atgctgtata atttgcacct tatttttacc
      901 tagtatctca accattatat aacttacact atcattaaca tacacattac gcagttcata
      961 atttgttccc aatatatgtt gaattacggg aactgtatcc catttccatg caatctgata
     1021 attttgcttt cccaaatgca ccgtttttcg taaaattgta tcatgttgta ttataagaaa
     1081 atacggcaat tcttttacca ttgaattgtt ttcaatagtt atagaaagta tttttggatt
     1141 atttttcttt gtttcgtgta gtttacttat atatgtttga aacacaaatg ctgcaaccaa
     1201 tactgagcat aaaaaaccta ttgtaattat tataaggttc ttatttgttt tgtttcgttt
     1261 cctatgtcgt tcaaattgaa tcatatttct gaaagtataa attacaatca aaaataacag
     1321 atttttattt acaattcgcc attatctgca aaactataat acccattgcg agtaacaatt
     1381 acatgatcga gcattgtaca atcgaaaaaa ggtgcagctt gttgaatttt cttagtaatt
     1441 tctttatctg cattacttgg atttgtattt cccgaaggat gattgtgtgc tactataata
     1501 ccggaagcta aatattccaa tgcagcttta cctatgattt ttacatcaat aacagtacct
     1561 gccactccac caacacttat tactttttga gcaattatag aattgtttct attaagatac
     1621 aacacccgaa attcttcatg tgccaaatct gccattgaag gatgcaacaa tctatacact
     1681 tgattactag atgtaatagg attagtaggt ttaacttctg tagcacttct ccgtttacca
     1741 agttcaaatg cagcaagcaa tgtgatagct tttgccggtc ctattccttt aatcttggtt
     1801 aaatcatgca acgaatatct tcctaattct tgcaaacaat tacctgataa atgaactatc
     1861 cgttttgcaa tatcgagagc cgattcgttt tgtgttccgg tagatataag tattgcaagc
     1921 aattctgcat ctgataatgc atgaactcct ttcaattgaa gtttctctcg cggacggtct
     1981 tcttctgccc acgatttaat ggataaaaaa tttgaattca tggtgttata ctaaaacaaa
     2041 agagttacac atctgcggca atagtaactc ttgaaaaata tattttttat ggtccggcaa
     2101 tactttgcaa ctgtttccaa ccaaaaccga ctttccaata ataaatttgt ccgttagctt
     2161 tattgtatcg cacatcacct gcttcacctg ttgtaagtgt tgaaatatca tttatggcag
     2221 ggatatcacc aacaaatgtc cacaatggag taccggaagt acctgcattg tacataaatt
     2281 tttgcttagt tgtattataa accaacaaac tatgagttgg tgatgctatt gcacttattt
     2341 gggccgaagt aagagttggt ataagcactc cggtattttg atttggtgcc acaatatgta
     2401 aaacagtagc agcattcggt gtttctgttg aaataccggt ttgagcatac attccaaaac
     2461 aaataaataa acttactata ctaaaaatat acttcatact atttatattt aaggagttat
     2521 agtaccggca ccaattgata tttgacgcca gttgttagca ttatttaaaa atattaaaac
     2581 atttcctgta gtatataatt ttgtttctcc ttcgtacata cccactttac tattagttga
     2641 cacactttct atatatgaaa atggacgcca ttctaatgaa ttataataca taaactcatt
     2701 acgagta
//
LOCUS       DAOTOW010000272         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29341_length_2706_cov_3.866088, whole genome shotgun sequence.
ACCESSION   DAOTOW010000272 DAOTOW010000000
VERSION     DAOTOW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29341_length_2706_cov_3.866088"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5243_05740"
     CDS             <1..205
                     /locus_tag="P5243_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18984.1"
                     /translation="STKRALAISYILLDNSKINGKRITVAGRGQFSPIDNSNTTAGRA
                     KNRRSEIILTPDLQELYNIIQNK"
     gene            299..1687
                     /locus_tag="P5243_05745"
     CDS             299..1687
                     /locus_tag="P5243_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HRS18985.1"
                     /translation="MKSSEATLLHVCKAVNHITDSEALHSVITNSEIPVSIRVNPNKI
                     QQTLPYEKVQWCSHGYYLPKRPIFTLDPLFHAGAYYVQEAASMFLEQIVAPYTYKPVK
                     VFDVCAAPGGKSTHVISLLQRGSLLVANEVIRSRAHILSENIIKWGKSNCVVTNNDPK
                     DFDFLSGFFDIVIVDAPCSGEGMFRKDVSTIQEWSPSHVDLCSKRQTRILHDVWNTLA
                     PGGILIYSTCTFNEIENEDVVSDFIAQTGATCKQISFFQDWNITEHEKKGAVCYRFFP
                     HKTKGEVFTISVICKHNGTEFEPPKRTPQAKNITIIPEKNRLHFSNLIQNYQSIVYER
                     QKGIIWSFAKDYRKFLLDIYMHCNVIHAGIPLVHIQGHKLNPLPGLAFANSFNSSILP
                     KLEVDLYTALRYFKKESLEISHMSKGWIQLTYKGVSIGFVKNIGNRANNPYPQEWSIK
                     MNVHQNQMWSIL"
     gene            complement(1701..2636)
                     /locus_tag="P5243_05750"
     CDS             complement(1701..2636)
                     /locus_tag="P5243_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS18986.1"
                     /translation="MNDTPEHKENEKESVQNENIQQEPIVSPKNNDELIIEIDEKQNI
                     EHTELNTSPSESISNYANEELEPEKEQIQEPQKKETKAERIARLKQAKHKKKEEPQQE
                     KLSRENNSKSKIGLILIVSLFIIVSAGAYLFVINPSILAKQFPSLVEKGIISIESETK
                     HTNETIAKTDSTDNTIITKEEIDTQNSSSVPEEEVVKTITKTLESQTPVKKNTQQTDS
                     QPIDRVVTKGKLQTPCWIISYSSISNEATAIKTVATLSTQGKKCGYYWIPDYVSNGPK
                     LFKVYIGPYNTKDAALTDLPQIKGEVSGAYVTEVK"
BASE COUNT          853 a          378 c          451 g         1024 t
ORIGIN      
        1 aagtactaaa cgtgctttgg caatatcata tatattactt gataattcaa aaatcaatgg
       61 aaaacgaatt actgttgcag gtagaggtca gttttctcca attgataatt cgaatactac
      121 cgcagggcgt gctaaaaata gaagaagtga aattatttta acgcctgatt tgcaagaatt
      181 atataatata attcaaaaca aataaatgtt ttgttttgag tttatactgt gtttcttttt
      241 gctaatagta tgtagtatat ttgtataaag aaacaatcat attatttgta attttttaat
      301 gaaatcttca gaagctactt tattgcatgt ttgtaaggca gttaatcata taactgattc
      361 tgaagcatta catagtgtaa ttacaaatag tgaaatacct gtttctatta gagttaatcc
      421 aaataaaatt caacagacgt taccctatga aaaagttcaa tggtgcagtc atggatatta
      481 tttacctaag cgtcctattt ttactttaga tccattgttt catgcaggag catactatgt
      541 gcaagaagct gcttcaatgt ttttggaaca aatagtagct ccgtatacat ataaaccagt
      601 aaaagttttt gatgtttgtg cagctccggg tggtaaatca actcatgtta tttcattgtt
      661 gcaaagaggc agtttgttgg ttgcaaacga agtaatacgc agtagagctc atattctatc
      721 ggaaaatatc ataaaatggg gaaagtcaaa ttgtgttgtt accaataatg atcctaaaga
      781 ttttgatttt ttatcaggtt tttttgatat agtaattgta gatgcgcctt gttcgggcga
      841 aggaatgttt cgtaaagatg tttccacaat tcaagaatgg tctccgtcac atgttgattt
      901 atgcagtaaa cgacaaactc gaattttaca tgatgtatgg aatactttgg ctccgggtgg
      961 tatacttatt tatagtactt gtacattcaa tgaaattgaa aatgaagatg tggtaagcga
     1021 ttttatagca caaaccggtg ctacttgtaa acaaatttcc ttttttcaag attggaatat
     1081 aactgagcat gaaaaaaaag gagcagtatg ttataggttt ttccctcata aaacaaaagg
     1141 agaagtattt actatttctg taatttgcaa gcataatgga actgaatttg aacctccaaa
     1201 acgtacccct caagcaaaaa atataactat aattcccgaa aaaaatcgtc ttcatttttc
     1261 taatttaatt caaaattatc aaagtattgt atatgaacga caaaaaggaa taatatggtc
     1321 atttgcaaaa gattatcgaa aatttctact cgatatatat atgcattgta atgtaatcca
     1381 tgcaggtatt cctttagttc atattcaggg ccataagctt aatccattac cgggtttagc
     1441 ttttgcaaat agttttaatt cttctatttt gcctaaactt gaggtagatt tatatactgc
     1501 gttacgatat tttaaaaaag aatcacttga aatttctcat atgtctaaag gatggataca
     1561 actaacctat aaaggtgttt caataggttt tgtaaagaat atcggtaata gagcaaacaa
     1621 tccatatcca caagaatgga gtataaagat gaatgtgcat caaaatcaaa tgtggtcaat
     1681 attgtaacgt aacatttaca ttattttacc tcagtaacat aagctccgct tacttctcct
     1741 tttatttgag gtaaatcagt taatgctgca tctttagtat tatatggtcc aatatatact
     1801 ttaaatagct ttggtccatt tgaaacatag tccggtatcc aataatatcc acatttttta
     1861 ccttgagtag aaagagttgc aactgtttta atggcagtag cttcgtttga aatacttgaa
     1921 tagcttataa tccaacaagg agtttgaagt ttacctttgg ttacaactct atcaataggt
     1981 tgtgagtctg tttgttgtgt gtttttctta acaggagttt ggctttctaa tgtttttgta
     2041 atagttttta ctacttcttc ttcaggtacc gatgaggaat tctgagtatc aatctcttct
     2101 tttgttatta ttgtattatc ggtagaatct gtttttgcaa tagtttcatt tgtatgttta
     2161 gtttctgatt ctatagaaat aatacctttt tcaactaaag atggaaactg tttagcaaga
     2221 atcgaagggt ttattacgaa taaatatgct cctgctgaaa ctataataaa taaagaaaca
     2281 atgagtatta atccgatttt actttttgaa ttgttttctc ttgaaagttt ttcctgttga
     2341 ggttcttctt ttttcttatg ttttgcttgt tttaatcgag caattcgttc tgctttggtt
     2401 tcctttttct gaggttcttg tatttgctct ttttccggtt ctagttcttc gtttgcataa
     2461 ttcgaaattg attctgaggg agaagtattt aattcagtat gttcaatatt ttgtttttca
     2521 tctatttcaa tgattaattc atcattgttt ttgggtgata caataggttc ttgttgaata
     2581 ttttcgtttt gaaccgattc tttttcattt tctttatgtt cgggagtatc attcatggtt
     2641 tttaatttta ggtttgttac aaatataaaa aatagcgtta accttttcaa tagctattta
     2701 aaagaa
//
LOCUS       DAOTOW010000273         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29638_length_2687_cov_2.996960, whole genome shotgun sequence.
ACCESSION   DAOTOW010000273 DAOTOW010000000
VERSION     DAOTOW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29638_length_2687_cov_2.996960"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /gene="tyrS"
                     /locus_tag="P5243_05755"
     CDS             <1..1108
                     /gene="tyrS"
                     /locus_tag="P5243_05755"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRS18987.1"
                     /translation="PIALIGGATGMIGDPSGKSQERNLLDEATLRHNQECIKKQLALF
                     LDFDSDKPNAAILVNNYDWMKEFSFLAFIRDVGKHITVNYMMSKDSVKKRLGEDAKMG
                     MSFTEFTYQLVQGYDFLYLYKQYNCKLQMGGSDQWGNITTGTELIRRKESGEAYALTC
                     PLITKSDGTKFGKTESGNVWLDPERTSPYAFYQFWLNVSDEDAQRYIKIFTMLTKTEI
                     DGLIAEHSQAPHLRILQKRLAQEVTCMVHGQQEYNAAVEASAILFGSGTAQSLAALDK
                     KTFLAVFDGVPTFQIPKATIETGIPILELLATHTNIFPSKGEARKMIQGGGVSINKSK
                     IDNPELAVDTTYIINNSYILIQKGKKNYYIVEII"
     gene            1105..2064
                     /locus_tag="P5243_05760"
     CDS             1105..2064
                     /locus_tag="P5243_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS18988.1"
                     /translation="MTVVRWGIIGPGKIAHKFVEGLRTVPNAQLYAVASSSIERARIF
                     AHEYGASCSYDSYIELVTNPKIDIIYIATTNNFHYEQILLCLQHNKPCVCEKPFTLNS
                     NQLHDVIQIAHNKQIFLMEALWTMFMPSVSTVKQHIQSNAIGIITHAHIDFGFEVPYS
                     AESRLFSPQLGGGALLDIGLYPVFLALHLFGEPHTVKAQMHKTELGVDVHDIIDFEYV
                     NNLTVHLEASFTHNLPCEAIITGTQGEIRMHRMWHCPCTISLTNSQGIHDITPKYSGN
                     GYNYEIQEAQHCIANNLTQSSIMPHSFSMLLMRYLDKIYTICS"
     gene            2134..>2687
                     /locus_tag="P5243_05765"
     CDS             2134..>2687
                     /locus_tag="P5243_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRS18989.1"
                     /translation="MEQKKRVYTFGNKQAEGNAEMKNLLGGKGANLADMNLIGIPVPP
                     GFTITTEVCTEYYQLGQAKVVELLKKEVEAAMAYVENIMGMKFGDKENPLLMSVRSGA
                     RVSMPGMMDTVLNLGLNDDAVEGIAKKTGNPRFARDSYRRFVHMYGDVVLEMKPESKE
                     DIDPFEEILEHMKESRGVQNDTDLS"
BASE COUNT          943 a          470 c          508 g          766 t
ORIGIN      
        1 acctatagca ttaataggtg gtgctaccgg tatgattggt gaccccagcg gcaaatcgca
       61 agaacgtaat ttgttagatg aagctacatt gcgacataat caagaatgta taaaaaaaca
      121 attggcatta tttcttgatt ttgattcgga taaaccaaat gccgctatat tggttaacaa
      181 ttacgactgg atgaaagaat ttagcttcct tgcctttatt cgtgatgttg gtaagcacat
      241 taccgtaaat tacatgatga gtaaagacag cgtaaaaaaa cgtttgggag aagatgccaa
      301 aatgggaatg tcgtttaccg aatttaccta ccagttggtg caaggctatg attttttata
      361 tttatataaa caatacaatt gcaaactgca aatgggtggt tcggaccaat ggggtaatat
      421 aactaccggt accgaactta ttcgtcgtaa agaatcgggc gaagcatatg ctcttacttg
      481 tccgcttatt acaaaatctg acggtacaaa attcggaaaa acagaatcgg gcaatgtatg
      541 gttagaccca gaacgtacat caccgtatgc attttatcaa ttttggctca atgtgtctga
      601 cgaagatgcc caacgatata ttaaaatatt taccatgctt accaaaaccg aaattgatgg
      661 tttaatagca gaacactctc aagcacccca tttgcgtata ttacaaaaac gattggcaca
      721 agaagtaact tgtatggtac acggccaaca agaatacaat gctgctgtag aagcttcggc
      781 tatactattt ggaagcggca cggcacaatc gttggctgcg ttagacaaaa aaacattttt
      841 ggcagtgttt gatggtgttc ctacttttca aatacccaaa gcaactattg aaaccggaat
      901 tcctatttta gaattacttg ctactcatac caacattttc ccaagcaaag gcgaagctcg
      961 taaaatgatt caaggtggcg gtgtaagtat taacaaaagc aaaatagata atcctgaatt
     1021 agctgttgac actacttata taataaacaa ctcatacatt ttaattcaaa aaggcaagaa
     1081 aaattattat atagtagaaa taatatgaca gttgttcgtt ggggtataat aggaccggga
     1141 aaaatagcac acaaatttgt agaaggacta cgaactgttc ccaacgcaca attatatgca
     1201 gtagcatcat catcaattga gcgagcgaga atttttgctc atgaatacgg agcctcatgc
     1261 tcgtatgatt catatataga attggtaacc aatcctaaga ttgatataat ttatatagct
     1321 acaaccaaca atttccatta cgaacaaata cttttatgct tgcaacacaa caaaccatgt
     1381 gtatgcgaaa aacctttcac actcaattct aaccagttgc atgatgtaat tcaaatagca
     1441 cacaataagc agatttttct catggaagcc ctgtggacta tgtttatgcc atcggtttct
     1501 actgtaaaac agcatattca aagtaatgca attggaatta ttactcatgc tcatattgac
     1561 tttggatttg aagtacccta ttctgccgaa tctcgattat tttctcctca attaggcgga
     1621 ggagcattgc tcgacattgg gttgtacccc gtgtttttag ctttacattt atttggagaa
     1681 ccacacacgg taaaagcaca aatgcataaa acagaattgg gggttgatgt tcacgacatc
     1741 attgattttg aatatgtaaa caatcttacc gtacatttag aagcatcgtt tactcataat
     1801 ttaccatgcg aagcaattat taccggaaca caaggagaaa ttcgtatgca tagaatgtgg
     1861 cattgcccat gcacaataag ccttacaaat tcacaaggca tacatgacat aacccccaaa
     1921 tatagtggca acggatacaa ttatgaaata caagaagcac aacattgcat agcaaataat
     1981 ctgacacaaa gcagtataat gccccattcg tttagcatgt tacttatgag gtatttagat
     2041 aaaatatata cgatttgctc gtaataaatt ctaaaaaaaa attatttttg tagagttaga
     2101 aaatcgaata attaatcaca ataaaaatta cgtatggaac aaaaaaaacg tgtgtacacc
     2161 tttggaaata agcaagccga aggtaatgct gaaatgaaaa atttattagg tggtaaggga
     2221 gcaaatttag ccgacatgaa ccttataggt attcctgtac ctccgggatt tacaataact
     2281 accgaagtgt gtaccgaata ttatcaatta ggacaagcaa aagttgtaga actattaaaa
     2341 aaagaagtag aagctgcaat ggcatatgtt gaaaatataa tgggaatgaa atttggcgac
     2401 aaagaaaacc cgttattaat gtcggttcgt tcgggtgcac gtgtttctat gcccggtatg
     2461 atggataccg tattgaacct tggattgaat gatgatgctg ttgaaggtat tgcaaaaaaa
     2521 accggtaatc ctcgttttgc aagggattca taccgcagat ttgttcacat gtatggcgat
     2581 gtggtattag aaatgaaacc ggaatcaaaa gaagatattg acccattcga agaaattctt
     2641 gaacacatga aagaatcgcg tggtgttcaa aatgatactg atttatc
//
LOCUS       DAOTOW010000274         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29696_length_2682_cov_4.773887, whole genome shotgun sequence.
ACCESSION   DAOTOW010000274 DAOTOW010000000
VERSION     DAOTOW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29696_length_2682_cov_4.773887"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="P5243_05770"
     CDS             complement(<1..491)
                     /locus_tag="P5243_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18990.1"
                     /translation="MARVLFFCTSSFPYGTGETFIENEIPFLSQAFDLIVIVSNDTIS
                     PQTRTIPQNVVCQRISYELPKFQKFLSIFGIFSKLFWQEMRIITKEYRKSITGIQIKT
                     AIQTLRKASYIGNYMSTIIQNYSSESDSRFVYSYWTNDMAFVNTKICQKLSVQSVFSR
                     AHR"
     gene            601..1932
                     /locus_tag="P5243_05775"
     CDS             601..1932
                     /locus_tag="P5243_05775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS18991.1"
                     /translation="MKKVLILAYDFPPYVSVGGLRPYSWFQYFHEFGVYPIVITRQWS
                     NEHRNFLDYIAPSESEEVEVGEFAHGTIIRTPYTQNISNKMLEKYGKNKYAFIRKIFT
                     AYYEIMQYHIQIGPKIEIYKAAQEYLKNNNVDCILTSGEPFILFRYAHLLSKEFKTPW
                     IADYRDPWTQDKKRSYDTISRLLNMFWEKRFVKSASIITTVTPFFKEKIQTLFPNKRF
                     EIIPNGYNPTSTSKVSNIPQNSDVLRISFVGTIYKWHPIESVLRVFQTFIHSQSTPPR
                     IELRFYGINNAEEYRNLILLRFNSLAKYVSIIPKIQNEQLMEYLAKDNVFLLFNYYSY
                     TGTKIYDYIALKRLILLCYTDDSEGNELKEKYYNIDEENSDFQQVQAEIITQTNSGII
                     VKDSTQLLHTFSELYSEFIQNGSISCNSVNTEQFSRKIQASNICSLIKEIC"
     gene            1934..2554
                     /gene="hisH"
                     /locus_tag="P5243_05780"
     CDS             1934..2554
                     /gene="hisH"
                     /locus_tag="P5243_05780"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRS18992.1"
                     /translation="MPQTIAIVDYNMGNIHSVYKKMTHVTSHVIVTNSAEDIAKADKI
                     ILPGVGHFAKAMEHIHSLGIYDSLQTHAIVKQKPILGICLGMQLFANISEEGNVAGLG
                     WIDAQVIRFRITNTLQYKIPHTGWNEAIQAKDSKLLHNIEQCSPFYFVHSFHYVCNNK
                     EDILQQTEYEYLFTSTVEKNNIFGVQYHPEKSHNDGTQLLQNFINL"
BASE COUNT         1035 a          409 c          378 g          860 t
ORIGIN      
        1 caacgatgag ctcgcgaaaa tacactttgt actgacaatt tttgacatat tttagtattt
       61 acaaacgcca tatcgttcgt ccaatatgaa tatacaaacc ttgaatcaga ctctgaagag
      121 taattttgta taattgtaga catataattg ccaatataag aagcttttcg aagcgtctga
      181 atcgcagttt taatttgtat gccggtaata gattttctat attcttttgt aattattctc
      241 atttcttgcc aaaaaagttt tgaaaaaatt ccaaatattg acaaaaactt ctgaaatttt
      301 ggcaattcat atgatattct ttggcataca acattttgag gtattgttcg agtttgaggt
      361 gagatagtat cattagaaac tattacgatt aaatcaaatg cttgtgaaag aaacggtatt
      421 tcattctcta taaaagtttc gccggtgcca taaggaaacg atgatgtaca aaaaaagaga
      481 acacgtgcca tacagtaaat ttatacaaat ataaaaaaaa cagcaaaata gcgattatac
      541 aaataatgca acaatacatt cagattagta tttttatata tttgtacaaa ttctcatgta
      601 atgaagaaag tattaatact tgcatacgat tttcccccat atgtttcagt aggaggttta
      661 cgaccatatt catggtttca atattttcat gaatttggag tatatcctat tgtaattaca
      721 agacaatgga gcaacgaaca tcgtaatttt ttagattata ttgcaccaag tgaaagtgaa
      781 gaagtagaag tcggagaatt tgcacacggt acaataatca ggactccgta tacccaaaat
      841 atttctaata aaatgctcga aaaatatggt aaaaataaat atgcatttat tagaaaaata
      901 tttactgcat actatgaaat aatgcaatat catattcaga taggaccaaa aatcgaaatt
      961 tataaagctg ctcaagaata ccttaaaaat aacaacgttg attgtatact tacaagtggc
     1021 gaacctttta ttttatttcg ttacgcacac ttactcagca aagaatttaa aactccatgg
     1081 attgctgatt atcgcgaccc gtggactcaa gataaaaaac ggagttatga tacaataagc
     1141 cgtttattaa atatgttttg ggaaaaacgg tttgtaaaat ctgcttcaat tataacaact
     1201 gtaacacctt tttttaaaga aaaaatacaa acattatttc caaacaaacg gttcgaaata
     1261 attccaaacg ggtataaccc aacatcaaca tcaaaagtta gtaacatacc ccaaaattca
     1321 gacgttctta gaataagctt tgtaggcacc atatacaaat ggcatcctat tgaaagtgta
     1381 ttacgagttt ttcaaacatt tatacattct caaagtactc caccacgaat tgaattacga
     1441 ttttacggaa taaacaatgc agaagaatat aggaatctta tattacttag atttaattca
     1501 ttagccaaat atgtttcaat tattcctaaa attcaaaacg aacaattaat ggaatattta
     1561 gccaaagaca atgtatttct gttatttaat tactattcat ataccggcac aaaaatttat
     1621 gattatatag ctcttaaacg tctcatttta ttgtgttata cggatgattc cgaaggaaat
     1681 gaattaaaag aaaaatatta taatattgac gaagaaaatt ctgattttca acaagttcaa
     1741 gcagaaatta taacacaaac aaactcagga attatagtta aagattctac acaattacta
     1801 catacttttt cagaattata ttcagaattt atacaaaacg gttccatttc atgtaattct
     1861 gtaaataccg aacaattttc aagaaaaata caagcatcaa atatttgttc attaattaaa
     1921 gaaatttgtt aatatgcctc aaacaatagc tatagtagat tataatatgg gtaatataca
     1981 ttcggtatat aaaaaaatga ctcatgtaac atcgcatgtt attgtaacaa actctgctga
     2041 agatattgca aaagccgata aaataatatt acccggcgta ggccattttg ctaaagctat
     2101 ggaacacatt cattctttag gaatatatga ttcattacaa actcatgcta tagtaaaaca
     2161 aaagcctatt ttgggaatat gcttgggaat gcaactattt gcaaatataa gtgaagaagg
     2221 aaatgtagca gggttaggct ggatagatgc acaggttata cgatttagaa ttacaaatac
     2281 attacaatat aaaattcctc acaccggatg gaatgaagct atacaagcaa aagacagtaa
     2341 attattacac aatattgaac aatgttctcc attctatttt gttcattcat ttcactatgt
     2401 ttgcaacaac aaagaagata tattgcaaca aaccgaatat gaatacttat ttacttctac
     2461 cgttgaaaaa aataatattt tcggtgttca atatcatcct gaaaaaagtc ataatgatgg
     2521 cacacaatta ttacaaaact ttattaattt gtaactatgt ttaaaccacg tttaataccg
     2581 gtattactgc tgcgaaacaa tattcttgta aaatcggttc gtttttctaa acataactat
     2641 ataggcgatc caataaatgc tgtaaaatta tttaacgatt ta
//
LOCUS       DAOTOW010000275         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_29890_length_2668_cov_3.282817, whole genome shotgun sequence.
ACCESSION   DAOTOW010000275 DAOTOW010000000
VERSION     DAOTOW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29890_length_2668_cov_3.282817"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2668
                     /locus_tag="P5243_05785"
     CDS             <1..>2668
                     /locus_tag="P5243_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS18993.1"
                     /translation="VALLYGLLLLPWTQAKLTSFVSSIISNEIGLQVHISHITPTLWK
                     TVFLHDITITNEFNDTVLTAKGCKATIDNIHLDSARVILQKVRFSDAHLSIIQDSAGI
                     WNIDNFLKVVVYSKGSKSWKVGINEIELRGISVHVASLRTKEPDNQQGIDFDNIQLTR
                     VNMNVFNFSKFKHSFAASISNFSCVEQSGFSIQYLQTDAVINDSSIVCKNTMLFTPRT
                     KFFSQHTAMYFKSFDDFDDFTQNVVLSSSIQYSQISFEDIAYFTSNAQHIPYNFTLSG
                     SVAGRISNIRGKNIHIGFNASSRFIGSFELSGLPDISQTFMHIHADVLETNKHDIQRL
                     RIPPYTQTVYVQLPSFLDKVETYSYTGNFTGFLNDFVAYGTLSTNIGTIYSDILISEL
                     SQTQSLYKYSGKITCKNLELDKFPVHENTWGTLNASVEVTGMYDAKNILTAQVNGVIS
                     DLEFYNYTYHDITIDGMLAKQKFDGNIQIQDNNLNLSFHGLFDYSTETPSFNFNATVT
                     HANLFALHLYDDTVSVVSLQTNVNFVGIQLDNLKGYIHIPYVQYTGKYGTYTSKQTSI
                     DIDNINQTRNVTIRSDLFDLTINGKGSYKELPVFFYDFLHSHIPSLPKQNFTAKKTFV
                     PSFNASLKIKKIDSLFQVLYPEIHIAQNTYINTVYSDESKLLVIQSHIPQFTYKDNTF
                     TDISIQTNGTVDNILTNIVFDYDIFKKNSIGIHISNDSIQTIVSWSNTDEKRYEGSLH
                     MVGAFAPSSHQFLPAIHLDVPKQSLYIADTLWNIYTSKIIIDSTEIYIPIGSFGKSNQ
                     RVTLEGYISENPHKVLQADFFNYNLENFNYIIDNKQVKIEGALEGRIEVKDVYHDMLL
                     FANVVAPQFSFNGHKLGKLTAVSQWIPLQKAIA"
BASE COUNT          920 a          383 c          401 g          964 t
ORIGIN      
        1 gttgcattac tctatggttt actattattg ccttggactc aagcaaaact tacatcattt
       61 gtttcgtcta tcataagcaa tgaaatagga ttgcaagtac atatatctca tattacacct
      121 actttatgga aaactgtgtt tttacatgat ataactatta caaatgaatt taatgatacc
      181 gtacttacgg ctaaaggttg taaagcaact atagataata tacatttaga ttctgcaaga
      241 gttatattgc aaaaggtgcg tttttcagat gctcatcttt ctattattca agattctgcc
      301 ggtatttgga atatagacaa ttttttgaaa gttgtagtat atagtaaagg ttcaaaatct
      361 tggaaagtag gtattaatga aatagaatta cgtggaattt ctgttcatgt agcatcttta
      421 agaactaaag aacctgataa tcaacaaggt attgactttg ataatattca attgactcgt
      481 gtaaatatga atgtgtttaa ttttagtaaa tttaaacatt cgtttgctgc atctattagt
      541 aatttttcat gtgttgaaca gtccggcttt tcaattcaat atctacagac cgatgctgtt
      601 ataaacgatt cttcaattgt atgtaaaaat actatgctgt ttactcctcg tactaaattc
      661 ttttcgcaac acacagctat gtattttaaa tcgtttgatg attttgatga ttttactcaa
      721 aacgttgtat tgtcaagttc aattcaatat tcgcaaattt catttgaaga tatagcatat
      781 tttacttcaa atgcacaaca tataccatat aattttactt tgtcgggtag tgttgccgga
      841 agaatttcaa atattcgagg caaaaatatt catataggat ttaatgcaag tagtagattt
      901 ataggttcat ttgaattatc cggtttgccc gatatttctc aaacgtttat gcatatacat
      961 gccgatgtgt tagaaacaaa taaacatgat attcaaagat tacggattcc tccttatacc
     1021 caaacggtat atgtgcaatt accatcattt ttggacaaag ttgaaaccta ttcatataca
     1081 ggtaatttta ccggtttctt gaatgatttt gtagcatacg gaacactctc aactaacata
     1141 ggtaccattt attccgatat tttaatttct gaattgtcgc aaacccaatc gttgtataaa
     1201 tatagtggta aaataacatg caaaaattta gaactcgaca aatttccggt acatgaaaat
     1261 acgtggggaa ctcttaatgc atcggttgag gttacaggaa tgtatgatgc aaaaaatata
     1321 cttactgctc aagtaaacgg ggttatttct gatttagaat tttataatta tacatatcat
     1381 gatattacaa tagatggtat gcttgcaaaa caaaaatttg atggtaatat tcaaatacaa
     1441 gataataatt taaacctatc gtttcatggt ttgtttgatt attctacaga aaccccaagt
     1501 tttaacttta atgctactgt aactcatgca aatttatttg ctcttcattt atatgacgat
     1561 accgtttctg tagtttcatt acaaaccaat gtgaactttg taggaattca acttgataat
     1621 ttaaaaggat atattcatat accgtatgtt caatatacag gtaaatatgg tacgtatacg
     1681 tcaaagcaaa catcaattga tattgataat ataaatcaga cacgtaatgt tactattcga
     1741 tcagatttgt ttgatttaac aattaatggt aaaggtagtt ataaggaatt accggtgttt
     1801 ttttatgact ttttgcattc tcatattcct tctttaccca aacaaaattt tactgctaaa
     1861 aaaacatttg ttccaagctt taatgcttcg cttaagatta agaaaattga tagtttattt
     1921 caggttttat atccagaaat tcatattgct caaaacacat atataaatac ggtgtattcc
     1981 gatgaatcaa aattgttagt aattcaatct catattccac aatttaccta taaggataat
     2041 acttttacag atatatctat acaaactaat ggaactgtag ataatattct tactaatatt
     2101 gtatttgatt atgatatttt taagaaaaat tctattggca ttcacatttc aaatgatagt
     2161 attcaaacaa tagtttcttg gagtaatact gacgaaaaac ggtatgaagg ttcattacat
     2221 atggttggtg catttgctcc cagttctcat caatttttac ctgctattca tttagatgtt
     2281 cccaagcaat cactctatat tgcagatacg ttgtggaata tttatacaag taaaattata
     2341 attgacagta cagaaatata tataccaatt ggttcttttg gtaaaagtaa tcaacgggtt
     2401 acattagaag gatatatttc tgaaaatccc cacaaagtgt tgcaagccga tttttttaat
     2461 tataatcttg aaaattttaa ttacataatt gataataaac aggtaaaaat tgagggggca
     2521 ttggaaggaa gaatcgaggt aaaagatgta tatcatgata tgttattatt tgcaaatgtt
     2581 gtagctcctc aattttcatt taatggtcat aaattaggta aattaacagc tgtaagtcaa
     2641 tggataccat tacaaaaagc tattgcta
//
LOCUS       DAOTOW010000276         2656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30065_length_2656_cov_3.284121, whole genome shotgun sequence.
ACCESSION   DAOTOW010000276 DAOTOW010000000
VERSION     DAOTOW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30065_length_2656_cov_3.284121"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="P5243_05790"
     CDS             complement(<1..393)
                     /locus_tag="P5243_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HRS18994.1"
                     /translation="MWKIFELYALISIVSWGIAMVLLAFRKPIVARISDALVLLGIIS
                     LSFFIVKLWMFLDRPPLRTLGETRLWYSLFISCIGYVLYKRWNYRLFLAYALLMALVF
                     MIINYAKPDIHDKALMPALQSVWFVPHVI"
     gene            complement(377..1621)
                     /locus_tag="P5243_05795"
     CDS             complement(377..1621)
                     /locus_tag="P5243_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRS18995.1"
                     /translation="MKSPSRTMWEQPWGYAEAFAIPVALFLGAAGFEFVTGKAAPTLS
                     FPTNVVVLLVLINVIIILHAASAKNTIIRWFSSIPASIGALTFFLAVSLIMALVPQQE
                     HPHSLFLFYNVTSSWLYYVATGYLLMVLGMVSVRRLFPVSKKNIGFFLNHLGLWITIT
                     AATFGAGDIEKYTMSVREGATEWRVMNANNTIQELDFAIQLKDFEIEEYPSKIGFVDM
                     RTGDVLEKNRKKAIFELDTIHGIRFNEYVITIKTYYPTSMFYVQDYFPLSDIGATQSY
                     YIEVSDTKRNKTYSDWIGAASMSQQAKYLILPDSIAIVALQPEAKKFSSRIRLYTKEG
                     STIDTIVEVNQPITCNSYTIYQTGYDEKMGRWSETSIFELVRDPWLPIVYVGIFMMIA
                     GALYIIWFGRTSKTKEQYVENI"
     gene            complement(1618..2106)
                     /locus_tag="P5243_05800"
     CDS             complement(1618..2106)
                     /locus_tag="P5243_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS18996.1"
                     /translation="MIHNCLQFTIAYFNKDMARIHHAMKVYTYACAIARAENLSEYNY
                     NTIAYTALLHDIGIVEAEKKYNSCSGKYQEIEGPAVARSFLETTNCSPQHIDRICYII
                     GHHHTYSAIDDIDFQILVEADFLVNIFEENIALPAIEHISKTYFKTEFGKQLLTQLYL
                     NI"
     gene            complement(2157..2507)
                     /locus_tag="P5243_05805"
     CDS             complement(2157..2507)
                     /locus_tag="P5243_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRS18997.1"
                     /translation="MNLSIDNKNVGFAHSQSHSLKQSAQYADGAIVSKIITKKETGNV
                     TLFAFDKGQFLSEHTAPFDATIQIVEGEAIVSIDRKDYTVREGEIIIMPANIPHAVFA
                     STQMKMMLIMIKSQ"
BASE COUNT          836 a          508 c          481 g          831 t
ORIGIN      
        1 aattacatga ggtacaaacc acacactttg caatgcaggc atcaacgcct tgtcgtgaat
       61 atcgggttta gcgtaattta ttatcataaa taccaatgcc ataagcagtg cataggcaag
      121 aaacaatcgg taattccatc gtttgtacaa tacatatcct atacaactaa taaaaagtga
      181 gtaccacaat cgcgtttctc ccaatgtacg caagggagga cggtctaaaa acatccacaa
      241 tttgacaata aaaaacgaga gcgatataat gcccaataat accaatgcat cggatatacg
      301 tgcaacaata ggtttacgaa atgcaagcaa taccatggca attccccacg atacgatgct
      361 aataagtgcg tataattcaa atattttcca catactgttc ttttgtttta gatgttctac
      421 caaaccaaat aatatacaat gcaccggcta tcatcataaa aataccgaca tacactatag
      481 gcaaccacgg gtcgcgtacc aattcaaaaa tacttgtttc cgaccaccgt cccatttttt
      541 cgtcgtagcc ggtttgatat atagtatatg aattacacgt aataggctga tttacttcaa
      601 caatagtatc gatggtgctt ccttcttttg tgtacaaacg aatacgcgac gaaaactttt
      661 ttgcctcggg ttgtaatgca actattgcta tagaatcggg caatattaga tattttgcct
      721 gttgcgacat gcttgccgca cctatccagt cgctataggt tttgttgcgt ttggtatcac
      781 ttacttcgat atagtacgat tgtgttgcac ctatatccga caatgggaaa tagtcttgta
      841 cataaaacat agacgtgggg taatatgttt tgatagtaat tacatattcg ttgaatcgaa
      901 taccatgaat agtatcgagt tcaaaaattg cttttttcct gtttttttct aatacatcgc
      961 cggtacgcat atctacaaac ccaattttcg aaggatactc ttcaatttca aaatctttga
     1021 gttgaatagc aaagtcaagt tcttgtatgg tattgttggc attcattacc cgccattcgg
     1081 tagcaccttc gcgtaccgac atcgtatatt tttctatatc tccggcacca aatgtagctg
     1141 cagtaattgt aatccacaac cccaaatgat taagaaaaaa tccaatattt tttttagaaa
     1201 caggaaataa tcggcgaaca cttaccattc ccaaaaccat aagtaaatat ccggtagcta
     1261 cataatacaa ccaactcgaa gtaacattgt aaaacaaaaa caaagaatga ggatgttctt
     1321 gttgcggaac caaagccata ataagcgata ccgcaagaaa aaaagtgaga gcccctatag
     1381 atgccgggat gctcgaaaac cagcggataa tggtattttt agcagaagct gcatggagta
     1441 tgattatgac attgataaga acaagcaata caactacatt ggtaggaaac gataaagttg
     1501 gggcagcctt gccggttaca aattcaaagc ccgcagctcc aagaaacaag gctacgggaa
     1561 ttgcaaatgc ttcggcatat ccccacggtt gttcccacat tgtgcgtgat ggtgatttca
     1621 tatgtttaga tataattgtg ttagtaattg ttttccaaat tctgttttaa aatatgtttt
     1681 gctgatatgt tctatagcag gtaatgcaat attttcttcg aatatattta ctaaaaaatc
     1741 agcttctacc agtatttgaa aatctatatc gtcaatagca gaataggtat ggtgatgacc
     1801 tatgatatag caaattctat cgatgtgttg tggagaacaa ttagtagttt ccaaaaaaga
     1861 acgagctacc gccggtcctt ctatttcttg gtacttgcct gaacaagagt tatatttttt
     1921 ctcggcttct actatgccta tatcatgcag caaggcagta tatgctatgg tgttatagtt
     1981 gtattccgag aggttttcgg cacgagcaat tgcacatgca taggtataca ctttcattgc
     2041 atgatgtatg cgagccatgt ctttgttgaa atacgcaatc gtaaattgta agcaattgtg
     2101 aatcatttgt acattttaaa aacaaatgta actaacaatc aaatacaaac atacaattat
     2161 tgcgatttta tcattataag catcattttc atttgagtac ttgcaaacac agcatgcggt
     2221 atgtttgccg gcattattat aatttcacct tcgcgtactg tgtaatcttt gcgatcgata
     2281 gatacgattg cttcgccttc tactatctgt atggtagcat caaacggagc agtgtgttcg
     2341 ctcagaaatt gccctttgtc gaatgcaaaa agtgttacat ttcccgtttc ttttttagta
     2401 ataattttac ttactattgc tccatcggca tattgtgccg actgcttgag cgagtgcgat
     2461 tgcgaatgtg caaatcccac gtttttgttg tctatggata gattcattgt aggtatgagt
     2521 tattagttat aaattgtgag ttcttagttt tgaattttga gttacacaga gagatagaat
     2581 tatgaaaaaa ctcaaaactc ataattatga attcaaaact tgtatcactc ccaccgggca
     2641 ctggtctgct gcttct
//
LOCUS       DAOTOW010000277         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30077_length_2655_cov_3.595769, whole genome shotgun sequence.
ACCESSION   DAOTOW010000277 DAOTOW010000000
VERSION     DAOTOW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30077_length_2655_cov_3.595769"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="P5243_05810"
     CDS             complement(<1..1285)
                     /locus_tag="P5243_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRS18998.1"
                     /translation="MKKIAILVVSALALASCSDDFLNKEPLAVGSNVTFYDTPGNCEL
                     AVNAIYDPLQWEEMYSRNFWAIADVASDDAEKGGGEDAPRYKDDQPPMFDISTYNVNQ
                     LNQYIIDMWKGYYIGIGRANAMLDQTGEFVTGKDSTVYKRMRGEARFLRAFYYFDMVR
                     IWGPVPLVTKTVSPGESYGIGNRASGDDAVGSKQMREIYNFIVDELTAIQNDVPWTYY
                     DAANFGRVTRGAVKALLAKVLLFRADVFSQNADYELAYQVAKGLITDPSNPHRLEPKY
                     QDIFDMDMLKENSPELLFSIQFIDAPKSDSYNRDGQGEGTIKPTYTGPRYFIKDDGSV
                     GSSGDFGYGFMMPRKDLVDQFDANDPRLDMMYKPHDSIYWDIPSKGNPKWYKIYYPSY
                     TTGYYNKKAGLNYNKFASLKAQSSGKDIPVIRYAEV"
     gene            complement(1315..>2655)
                     /locus_tag="P5243_05815"
     CDS             complement(1315..>2655)
                     /locus_tag="P5243_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS18999.1"
                     /translation="DASSRFGPNQKWGNFPSVGFAWKVNKESFFYNNELLKNNVSLFK
                     IRGGWGKIGNENIGNYRYVASVNSNDSRSGYNFGGTAVPGATPISVPNADVHWEEATS
                     TNIGAEINLWKNKLMFTADYYVKNNVDNLIYITVPGVVGIDGSGNNPVSNEGKVRNQG
                     VEFSTTYRSSYKGDSSKYELTYNAGFNFTYNINEVTYQETPRQGGRVDRVGFTANTLT
                     GYPIAGFWGYKVDGIYQNWEEVNAGAQPSAKPGDYKIVDVNGDGQITTDDITYLGSPH
                     PDFTYGLNANLGYAGFDLGISFQGVYGNKIYNATKWYLDGGYLGSNYSTRRLDVWAPD
                     NTDSDQPTDPNWFTGKEQRAVSSAFIEDGSYLRLKNITLGYTLPTKYAEKIKLSNVRV
                     YGQVQNALTFTKYSGFDPEIGTNTELNYEGPEFGVDRGVYPQARSVVFGVNLQF"
BASE COUNT          854 a          537 c          372 g          892 t
ORIGIN      
        1 acacttcagc ataacgtatt accggtatat ctttaccact ggattgtgct ttgagtgaag
       61 caaatttatt ataattaaga cctgcttttt tattataata tcctgtagta tatgatggat
      121 aatagatttt ataccatttt ggatttcctt tacttggtat atcccaatat atactatcat
      181 gcggtttgta catcatatct aaacgagggt cgtttgcatc aaattgatca accaaatctt
      241 tacgaggcat cataaaacca tatccgaagt ctccagacga accaacagaa ccatcatctt
      301 taataaaata acgaggacca gtatatgttg gtttaattgt tccctctcct tgaccatcac
      361 gattataact atcagatttt ggagcatcta taaattgaat agagaaaagt aattcaggac
      421 tattttcttt taacatatcc atatcaaaaa tatcttgata ttttggttct aatctatgag
      481 gattactagg atctgtaatt aatccttttg ctacttgata tgccaactca tagtctgcat
      541 tttgtgaaaa cacgtctgct ctaaacaata aaacttttgc caacaatgct tttacagcac
      601 cacgagttac acgaccaaaa ttagcagcat cataatacgt ccatggtaca tcattttgta
      661 tagcagttaa ttcatctaca ataaagttat agatttcacg catttgctta gacccaacag
      721 catcgtcacc ggaagctctg tttccaattc cataagactc tccaggacta acagttttag
      781 ttactagagg tacaggcccc caaatacgaa ccatatcaaa ataataaaat gcacgtaaaa
      841 atcgagcttc gcctctcata cgtttataca ctgtactatc tttacctgtt acaaactcgc
      901 cggtttgatc aagcattgca ttagcgcgtc caattccaat atagtaacct ttccacatat
      961 ctataatgta ctgattcaat tgatttacat tatatgtaga aatgtcaaac attggaggtt
     1021 gatcgtcttt atatcgagga gcatcttctc ctccgccttt ttctgcatca tcagaagcaa
     1081 catcggcaat agcccaaaag tttcgagaat acatttcttc ccattgtaat gggtcataga
     1141 ttgcatttac tgcaagttca caatttcctg gagtatcata aaacgttacg ttactcccta
     1201 cagcaagcgg ttctttattt aagaagtcat cagagcatga tgctaatgct aatgctgata
     1261 ctactaatat tgctattttt ttcatattat ctgtgttgta ttaaatttat accattaaaa
     1321 ttgtaggttt acaccaaata ctaccgaacg agcttgtgga tacacacctc tatcaactcc
     1381 aaattcagga ccttcataat tcaattctgt atttgtacct atttcagggt caaatccgga
     1441 atatttagtg aatgtaagtg cattctgaac ttgaccatat acacgaacat tacttaattt
     1501 aattttctct gcatattttg ttggtaatgt ataccccaaa gttatatttt tcaaacgcaa
     1561 atatgatcca tcttcaataa atgcagaaga aactgcacgt tgttcttttc ctgtaaacca
     1621 gtttggatct gttggttgat ctgaatctgt attatcagga gcccacacat ctaaacgacg
     1681 agtagagtag tttgaaccaa gataaccacc atctaagtac catttagttg cattgtaaat
     1741 tttattacca tatacacctt ggaaagaaat tcctaaatca aatcctgcat aacctaaatt
     1801 tgcattcaaa ccatacgtaa aatcaggatg aggagaacct aaataagtaa tatcatctgt
     1861 agtaatttgt ccatcaccgt ttacatcaac aattttataa tcaccgggtt tagcacttgg
     1921 ttgtgcacct gcattaactt cttcccaatt ttgataaatt ccatcaactt tatatcccca
     1981 aaatccggct attggataac ctgtaagtgt attagctgta aaacctacac gatcaacacg
     2041 accaccttga cgtggtgttt cttggtaagt aacttcatta atgttatatg tgaaattaaa
     2101 tccggcatta tatgttaatt catatttact tgaatctcct ttataagaac ttctataagt
     2161 tgttgagaat tcaacacctt gattacgaac tttaccttca tttgatacag ggttatttcc
     2221 tgaaccatca ataccaacaa caccaggaac agtaatataa attaagttat caacattatt
     2281 ttttacataa taatcagcgg taaacattaa tttgtttttc cataaattaa tctcagctcc
     2341 tatatttgta cttgtagctt cttcccagtg aacatcggca tttggtacag aaataggtgt
     2401 agctcccggc acagctgttc ctccaaaatt ataccctgaa cgactatcat ttgaatttac
     2461 cgaagcaaca tatcgataat tacctatatt ttcatttcca attttacccc atccaccacg
     2521 aattttaaat aacgatacat tattttttaa caactcatta ttatagaaga aagattcttt
     2581 attaactttc catgcaaaac ctacagatgg gaaatttccc catttttgat ttggtccgaa
     2641 acgtgaagaa gcatc
//
LOCUS       DAOTOW010000278         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30212_length_2647_cov_3.330247, whole genome shotgun sequence.
ACCESSION   DAOTOW010000278 DAOTOW010000000
VERSION     DAOTOW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30212_length_2647_cov_3.330247"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2647
                     /locus_tag="P5243_05820"
     CDS             <1..>2647
                     /locus_tag="P5243_05820"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS19000.1"
                     /translation="SSDTSQVVKIPIESPTSEYVRINCLLSISDSVYYVGTSYGLLQL
                     VIPKNGSPSVQKICNEIGDEFISSLTYDGIGNVWIGTGYKGVWILSKNLQLQQMPLLK
                     DLNSTTSGVTVIFKDSQNRMWIGTPGNGLFLYNPLTSYIHHIDETKGLHNTIVSAIIV
                     DEQENIWVGTDGGGINIYSKNMQLLHHLSKSTGLPSDAVLSFVQNSTHIWISTWRGGI
                     ASIDKKTFAIRYFNEDNSDLLFNGVKSIAWYAPDTLVVGTHGEGLQLFNTRTCSWIQD
                     FKIQYSSYFPDEQKFITQVAVDNNKKIWVATIRNLYCIYNKTVKDVLESDNFRYPQNP
                     MYITSISVDKAGYIIAATNKGVYRINTANYTIEKILTTFPEFVETVNLSVCVVNSEQY
                     WIANTKGLFVYNPKTKELQKRMLAAHASQRFFVPRAMYVDPKGYLYIGTCQGMYAFAL
                     RDIVNKQTQMKLEFAHLYINHVKAIPGGSILEQSLLHTKQLLIPYTTQNKSITFRIIS
                     FEFPETIEFAYKFEGFDKEWQYLGSNKEIHCNNLLPGTYTLRVKARQTVSNSSAEISM
                     SVVILPPWWKTWWFIGICLCIAIVGLWLLYKLRMYRIQRERKLLQIEVNSQLKAIQQQ
                     KECIEEQRNELQFVSDKLEESNSMLLMQNQELEELAKQLQEESLEISQLNESLKQSNI
                     TKEQLFAMITHDIYNSFDSLGILANKLQSYIAADSHKKPIIQTISQTISSHKELLKNL
                     IIWAKNQTNSIDVNLESIRIDSIMQELVTSIDSVAQEKNCTISIKNKDTETVYADSQL
                     VLIALRTLVYSIIQNTASGSFILISAETKKEQVEIQVHVQEIDEQLLMNALQFKKQIK
                     KQNNYSLDSQNLHIVVSQQCIEKCNG"
BASE COUNT          991 a          355 c          456 g          845 t
ORIGIN      
        1 tatcgtctga cacatcacaa gttgtaaaaa ttcctattga gagtccaact tcagaatatg
       61 taagaataaa ttgtttgttg agcatatcag atagcgtata ttatgttggt acatcatatg
      121 gacttttaca gttagttata ccaaaaaatg ggagtccttc agttcaaaaa atttgtaatg
      181 aaattggtga tgagtttatt tcttctttaa cctatgatgg aataggaaat gtgtggatag
      241 gaaccgggta taaaggagtt tggattttat caaaaaattt acagttacaa cagatgccgt
      301 tgttaaaaga tttaaattct acgacttccg gagtaaccgt aatctttaaa gattctcaaa
      361 ataggatgtg gataggaaca cccggcaatg gtttgttttt gtataatccg cttacatcat
      421 atatacatca tattgacgaa acaaaaggac tacataatac aattgtaagt gctataattg
      481 tagatgagca agaaaatata tgggttggaa cagatggtgg cggaattaac atatattcta
      541 aaaatatgca attactacat catttatcaa agagtaccgg attaccctcc gatgctgttt
      601 tatcatttgt gcaaaattct acacatatat ggatttctac atggcgtggc ggtattgcaa
      661 gtattgataa aaaaacgttt gctattcgat attttaacga agataattct gatttgcttt
      721 ttaatggagt aaaaagtatt gcttggtatg ctccggatac attagtggtt ggaacacatg
      781 gtgaaggatt acaattattc aatactcgca catgttcatg gattcaagat ttcaaaatac
      841 aatacagttc gtattttcct gatgaacaaa agtttataac acaggttgcc gttgataaca
      901 acaaaaaaat ttgggttgca actattcgaa atttatattg tatttacaac aaaactgtta
      961 aagatgtatt agaatccgat aattttagat atccacaaaa cccaatgtat attacatcta
     1021 ttagtgtgga taaagctgga tatattatag ctgcaaccaa taagggggta tatagaatta
     1081 atactgctaa ttatacaatt gaaaaaattc ttactacgtt tcccgaattt gttgaaacag
     1141 ttaatttgtc ggtatgtgtt gtaaattcag aacaatattg gatagccaat acaaaaggat
     1201 tatttgtata taatcctaaa acaaaagaat tacaaaaacg catgcttgca gctcatgcta
     1261 gtcaaagatt ctttgtacca cgagctatgt atgtcgatcc aaaaggttat ttatatatag
     1321 gaacatgcca aggtatgtac gcgtttgcgt tgcgtgatat tgtaaataaa caaacacaaa
     1381 tgaaattaga atttgcacat ttgtatataa atcatgtaaa agctataccg ggcggtagca
     1441 ttttagaaca atcgttgttg catacaaaac aattattaat accatataca acacaaaata
     1501 aaagtattac ttttagaatt ataagttttg aatttccgga aaccattgag tttgcatata
     1561 aatttgaagg ttttgataaa gaatggcaat atttaggatc caataaagaa attcattgca
     1621 ataatttact tcccggcact tacactttaa gagtaaaggc acgacaaaca gtaagtaata
     1681 gtagtgccga aatttccatg agcgttgtta tattacctcc ttggtggaaa acatggtggt
     1741 ttataggaat atgtttatgt atagcaatag tggggttatg gttgctttat aaattgagaa
     1801 tgtatagaat tcaacgtgaa cgtaaattgt tgcaaataga agtaaatagc caattgaaag
     1861 caatacaaca acaaaaagaa tgtattgaag aacaacggaa tgaattgcaa tttgtaagtg
     1921 ataaattaga agagagtaat tctatgttgc tcatgcaaaa tcaggaatta gaagaacttg
     1981 ccaaacagtt gcaagaggaa tcgttagaaa tttcacaatt aaatgaatca ttgaagcaaa
     2041 gtaatattac caaagaacaa ttatttgcaa tgattacgca tgatatttat aattcttttg
     2101 attcgctagg tatattggca aataaattac aatcctatat tgctgcagat tcgcataaaa
     2161 aacctattat tcaaactatt tcccaaacta tttcaagcca taaggaattg cttaaaaatt
     2221 taataatttg ggctaaaaac caaactaata gtatagatgt aaatttagaa tcaattagaa
     2281 ttgattctat aatgcaagaa cttgtaactt ctattgattc tgttgctcaa gaaaaaaatt
     2341 gtactataag tattaaaaat aaggatacag aaacggtata tgctgatagt caattggtgt
     2401 taatagctct tagaacttta gtgtattcta tcatacaaaa tactgcgtca ggtagtttta
     2461 ttcttatatc ggccgaaact aaaaaggaac aggttgaaat acaagtgcat gttcaggaaa
     2521 tagatgaaca actgttaatg aatgccttac agtttaaaaa gcaaataaaa aaacaaaata
     2581 attattcttt agattctcaa aatttgcata tagttgtatc tcagcaatgt attgaaaaat
     2641 gcaatgg
//
LOCUS       DAOTOW010000279         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30255_length_2644_cov_3.184627, whole genome shotgun sequence.
ACCESSION   DAOTOW010000279 DAOTOW010000000
VERSION     DAOTOW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30255_length_2644_cov_3.184627"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="P5243_05825"
     CDS             <1..392
                     /locus_tag="P5243_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRS19001.1"
                     /translation="SDGENKEKAQMARIEYSSALHLFPQHPSGINPEVFVCSALEHQG
                     LEKIWNHIQTYMDKTKQNNFFTTKRHTQASFWMYETIHDYLKQNFYSHTGIVSKIKEY
                     EQLVIQGNMSSFKAAQELLDLYTSQSK"
     gene            405..2069
                     /locus_tag="P5243_05830"
     CDS             405..2069
                     /locus_tag="P5243_05830"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRS19002.1"
                     /translation="MNIAIAQLNYHIGNFEQNAQNIIAHIRKAQSEGADIVVFSELSV
                     CGYPPLDLLEQRDFVEACLQTVQTIALECIEIAAVVGCPTLNEGEKGKRLFNSALFLY
                     NGSMCAQFNKTLLPTYDIFDEYRYFEPNTDFTVIEFKGKKIAITICEDLWDNQVVETA
                     FAKDKIYKTSPLEILSTYSPDFVINIAASPFSYNQDYLRSKVLQDNASLYKLPIIYVN
                     QVGANTEIIFDGCSKIVNSQGKIVYELAEFQEDFYVFDVENISQMLFVQEEPYNKIKK
                     IHDALILGIRDYFRKNNFTSAVLGLSGGIDSAVVLALVVEAIGAEHVRVLLMPSEFSS
                     SHSVDDAVALARTLQVQYDIIPIHSMYNATIQSLSPVFANKPFNVAEENIQARLRGVL
                     LMGISNKFGAILLNTSNKSEAAVGYSTMYGDMNGSLSILGDVYKTDVYKLAEYINKET
                     EIIPHNTIIKPPSAELRPNQKDSDSLPDYDTLDAILFEYIEKKLSTHEIIEQGFAAET
                     VLKTVRLVNANEYKRFQFPPILRVSSKAFGFGRRIPLVAKHEFDTI"
     gene            2097..>2644
                     /locus_tag="P5243_05835"
     CDS             2097..>2644
                     /locus_tag="P5243_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS19003.1"
                     /translation="MIIKAKHRSFYILFLVFVIYLIDFADRKVMSVLFESIKYDWNIT
                     DVQLSLLEGVTSLVIAVCVLPMAIAIDRWSRKYMIAIMVAAWSIITFVCAFAENYTQL
                     LILRALIGIGEAAYAPAAVSLLSKSFPARHRAKYIGIFDAAAPLGVALGMIVGGYIGM
                     IYGWRHAFGIVAIPGIIAPFEQ"
BASE COUNT          874 a          375 c          486 g          909 t
ORIGIN      
        1 aatctgatgg cgaaaataaa gaaaaagctc aaatggcaag aattgaatat tcatcggctt
       61 tgcatctttt ccctcagcat ccgtcaggca taaatcccga agtttttgtt tgttctgcac
      121 tcgaacatca aggcttagaa aaaatttgga atcatattca aacatacatg gataagacaa
      181 aacaaaataa tttttttaca accaaacgtc atactcaagc ttcattttgg atgtatgaaa
      241 ctattcatga ttatttaaaa caaaattttt attctcatac aggtatagta agtaaaataa
      301 aagaatacga acaattagta attcaaggca atatgagttc ttttaaagca gctcaagaat
      361 tattggattt gtatacatct cagtcaaaat aattagtgtt tcacatgaat atagctattg
      421 ctcaattaaa ttatcatatt ggaaattttg aacaaaatgc acaaaatata attgcacata
      481 tacgaaaagc acaatcagaa ggagctgata ttgttgtttt ttctgaatta tctgtttgtg
      541 ggtatcctcc gcttgattta cttgaacaac gtgattttgt agaagcttgt ttacaaacag
      601 tgcaaactat tgctcttgaa tgtattgaaa tagctgctgt ggttggatgt cctacattaa
      661 atgaaggtga aaaaggtaaa cggttgttta attctgcctt gtttttatac aatggttcta
      721 tgtgtgctca gtttaataaa acattgctgc caacatatga tatttttgat gaatacaggt
      781 attttgaacc caatacagat tttactgtaa tagaatttaa aggtaaaaaa atagctatta
      841 ccatttgtga agatttatgg gataatcagg tggtagaaac tgcatttgca aaagataaaa
      901 tttataaaac ctctccttta gaaatattga gtacatattc ccccgatttt gttataaata
      961 ttgcagcatc gccattttca tataatcaag attatttacg aagtaaagtt ttacaagata
     1021 atgcttcact atataaactg cctattattt atgtaaatca agttggggca aatactgaaa
     1081 ttatatttga tggttgttct aaaattgtaa attctcaagg taaaattgta tatgaattgg
     1141 cagaatttca agaagatttt tatgtgtttg atgtagagaa tatatcccaa atgctttttg
     1201 ttcaagaaga accatataat aaaataaaaa aaattcacga tgcgttgatt ttgggtattc
     1261 gtgattattt cagaaaaaat aactttacct cggcagtatt aggactatcg ggaggaatag
     1321 attctgccgt ggttttggct cttgtagtag aagcaatagg ggctgaacat gtgcgtgtat
     1381 tacttatgcc ttcagaattt tcaagttctc attctgttga tgatgctgta gctttggctc
     1441 gtacattaca ggtgcagtat gacattattc caatacatag tatgtacaat gccactatac
     1501 aaagtctttc tcctgtattt gcaaataaac cgtttaatgt tgccgaagaa aacattcaag
     1561 ctcgattgcg aggagttttg ttaatgggaa ttagtaataa atttggtgct attttgctta
     1621 atacatcaaa taaaagtgaa gctgcggttg ggtatagtac tatgtatggc gatatgaacg
     1681 gtagtttatc tattttgggt gatgtatata aaaccgatgt gtataaatta gcagagtata
     1741 tcaataaaga aacagaaatt attccacata atacaattat aaaacctccc tcggctgaac
     1801 taagaccaaa tcaaaaagat tctgattctt tacctgatta tgatacgtta gatgccattt
     1861 tatttgaata tattgaaaaa aaattatcaa ctcatgaaat catagaacaa ggatttgctg
     1921 cagaaacagt attgaaaaca gttcgtttgg ttaatgcgaa tgaatacaaa cgctttcagt
     1981 ttcctcctat tctcagagtt tcttcaaaag catttggatt tggcaggcga attccactgg
     2041 tggcaaaaca tgaatttgat actatataag gatgctgtgt aaagaatgaa ttttgtatga
     2101 ttataaaagc taaacatcgt tcgttttata tcttattttt agtttttgta atatatctta
     2161 ttgactttgc cgatagaaaa gtaatgtcgg ttttgtttga atctattaaa tatgattgga
     2221 atattaccga tgttcaatta agcttgttag aaggggttac ctcattagtt attgcagtat
     2281 gtgtattgcc tatggcaata gctattgaca gatggagcag aaaatacatg attgctataa
     2341 tggttgctgc atggagtatt ataacatttg tatgtgcttt tgcagaaaat tatactcaat
     2401 tactcatact acgtgcgttg ataggtatag gcgaagctgc ttatgctccg gcggcggttt
     2461 cacttttatc taaatcgttc ccggcacgac atagagcaaa atatatagga atttttgatg
     2521 ctgctgctcc gctgggagtt gcacttggta tgattgtagg tggttatata ggaatgattt
     2581 acggttggcg tcatgcgttt ggtattgttg ctatacccgg aattattgct ccctttgagc
     2641 aaga
//
LOCUS       DAOTOW010000280         2639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30332_length_2639_cov_2.847910, whole genome shotgun sequence.
ACCESSION   DAOTOW010000280 DAOTOW010000000
VERSION     DAOTOW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30332_length_2639_cov_2.847910"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..867
                     /gene="nadD"
                     /locus_tag="P5243_05840"
     CDS             292..867
                     /gene="nadD"
                     /locus_tag="P5243_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRS19004.1"
                     /translation="MKVGLFFGSFNPIHIGHLAIANYMVEFTNLDELWFVITPQNPAK
                     QKSTLLADSQRYYMVELAIEPFTKMKASRIEFTLPQPSYTIHTLIHLHELYPQHSFAL
                     IMGSDNLQTFNSWKNYKEILDSYSIYVYNRPNTQIPDEFKHHPAIEYVEAPLMEISSS
                     FIRQSIKQGKNIRGFMPESAWKYLDEMNFYK"
     gene            914..1597
                     /locus_tag="P5243_05845"
     CDS             914..1597
                     /locus_tag="P5243_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRS19005.1"
                     /translation="MLSSILLEGIIIGILVSIPMGPIGVLCVQRTVHQGRRSGLISGL
                     GAAVADSSFAFIAGFGLTFLTDFFKSQQFYLMFGGAIVLIFLGFRLFFTDTIKEVKKY
                     RIQRANPYTDFMSVFLLTLSNPITILFFGLVFTGLGVVKENIVQTGTLIGGIFLGAIC
                     WWFILTTVVSYFRNLFKIRIIFWINKVAGMLIVLFGALAMFNAFYPQFNNEKIQKSPI
                     VNMSNKLKK"
     gene            1601..>2639
                     /gene="mnmE"
                     /locus_tag="P5243_05850"
     CDS             1601..>2639
                     /gene="mnmE"
                     /locus_tag="P5243_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRS19006.1"
                     /translation="MVLESETICAIATAPGKGAIATIRLSGSDSKQIIQNIFKPSKKT
                     EQWYNIGYSLHYGSIYYNNELLDDVLVSIFNAPHSYTGEDSVEISCHGSVYIQNEILK
                     LLLHHGARLAKPGEFTQRAFLNGKLDLSQAEAVADLIASTSAASHKLALSQIKGGYAK
                     ELQELRLALLQFLSMIELELDFSEEEVEFADREQLRILANNLEHRVAKLLESFSIGNA
                     IKTGVPVCIVGEPNVGKSTLLNTILNEEKAIVSDIPGTTRDVIEDTMVIGGKTFRFMD
                     TAGIRDTSNIIEQLGIDRTFAKIDAASIVLFLIDVTSPLNYIKKNIASIKRKIAADKK
                     LYIVVNKIDLIT"
BASE COUNT          903 a          358 c          442 g          936 t
ORIGIN      
        1 ggttgaatgc gtgaaactaa ttgagcttca gaacctttat cgatttcggt tacaaaatca
       61 ttagtccctt ttgtttctat ttgactttgt tgtaaatgta aaccgctttg tctgatatat
      121 tcacctacag attttactat atcaatagtt tgaaagcata tactttgtaa atccatattt
      181 tgtatttttc ttcaaatgta ctatttttac agctaatgaa aatagtgtgt aaaaaaatat
      241 tttttatttt ttgttgtttt taaacaaaca tttgttgata gaattttgtt gatgaaagta
      301 ggcttgtttt ttggttcgtt taatccaatt catattggtc atttagctat agctaattac
      361 atggtagaat ttactaacct tgatgaatta tggtttgtta taactcctca aaatcctgca
      421 aaacaaaaat caacattact tgcagattct caacggtatt atatggttga attagcaata
      481 gaaccgttta caaaaatgaa agcaagccgc attgaattta cattgcctca accttcatat
      541 actattcata cattaattca tttgcatgag ttgtatccac aacatagttt tgctcttatt
      601 atgggaagcg ataatttgca aacatttaat tcttggaaaa attataaaga gatattagac
      661 tcatatagta tatatgtgta taatcgtcca aatactcaaa ttcctgatga atttaaacat
      721 catcctgcca ttgaatatgt agaagctcct cttatggaaa tttcatcgag ttttatacga
      781 caaagtataa aacaaggtaa aaatataaga gggttcatgc ccgaaagtgc atggaaatat
      841 ttagatgaga tgaattttta taaataaaaa aaatgtagta taattgttcc taattaaata
      901 aatactctac agcatgttat catcaatact attagaaggt ataattatag gaattttagt
      961 ttcaattcct atggggccta taggagtatt gtgtgtacaa cgaactgtac atcaggggcg
     1021 aagatcgggg cttatttctg gtcttggagc agctgttgcc gattcttcat ttgcttttat
     1081 agcaggattt ggattaacat ttttaactga tttttttaaa tcacaacaat tttatttaat
     1141 gtttggagga gctattgtac ttatattttt aggatttcga ttgtttttta ccgatacaat
     1201 taaagaagta aaaaaatatc gtatacaacg tgccaaccca tatactgatt ttatgtcggt
     1261 ttttttgttg acattgtcaa atccaattac aattttgttt tttggattag tgtttacggg
     1321 tcttggtgta gtaaaagaaa atatagtgca aacaggtacc cttataggag gaatatttct
     1381 tggagctatt tgttggtggt ttattcttac aactgttgtt tcatactttc gcaatttatt
     1441 taaaattcgc attatatttt ggataaataa agttgccggt atgttaatag tattatttgg
     1501 agctttagct atgtttaatg cgttttatcc acaatttaat aatgaaaaaa tacaaaaatc
     1561 gccaattgta aacatgtcta ataaattaaa aaaataaact atggttttag aatcagaaac
     1621 aatatgtgct atagctacgg cacccggtaa gggagcaata gcaacaatac gtttgagtgg
     1681 ttctgattca aaacagatta ttcaaaacat atttaaacct tcaaaaaaga cagaacaatg
     1741 gtataatata ggctatagtt tacattatgg gtctatatac tacaataatg aattattaga
     1801 tgatgtatta gtttcaatat ttaatgcacc tcattcatat acgggcgaag attctgttga
     1861 aattagctgt catggttctg tatatataca aaatgaaata ttaaaattat tgttgcatca
     1921 tggtgctcga ttagccaaac cgggtgaatt tactcagcgt gcatttttga acggaaaact
     1981 ggatttgtca caagccgaag cagttgccga tttaatagca tcaacttctg cagcatctca
     2041 taaattagca ttatcgcaaa ttaaaggtgg atatgccaaa gaattgcaag agcttaggct
     2101 ggcattatta caatttctat caatgataga attagaatta gattttagcg aagaagaagt
     2161 ggaatttgct gatagagagc aattacgcat attggcaaac aatttagaac atcgtgttgc
     2221 aaaattatta gaatcatttt ccataggtaa tgctattaaa acaggagtac ctgtgtgtat
     2281 agttggtgaa ccaaatgtag gaaaatcaac actgcttaat actattttga atgaagaaaa
     2341 ggctattgta tctgatattc cgggtactac tcgcgatgtt attgaagata ctatggttat
     2401 aggtggaaaa acatttcgtt ttatggatac tgcaggtata agagatacca gtaatatcat
     2461 agaacaatta ggaatagaca gaacatttgc taaaattgat gcagcatcta ttgttttgtt
     2521 tcttatagat gttacatcac cattgaatta cattaaaaaa aatattgctt caattaaacg
     2581 taaaattgct gccgataaaa aattatatat cgttgttaac aaaattgatt taataaccg
//
LOCUS       DAOTOW010000281         2630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30482_length_2630_cov_2.704466, whole genome shotgun sequence.
ACCESSION   DAOTOW010000281 DAOTOW010000000
VERSION     DAOTOW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30482_length_2630_cov_2.704466"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2431)
                     /locus_tag="P5243_05855"
     CDS             complement(<1..2431)
                     /locus_tag="P5243_05855"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS19007.1"
                     /translation="MKQSVYVVTIVLFALFENVLYSQTSSLNSDFELGNFTGWTASNG
                     SWNRTTGVITFTNNTITTGRQTIISNLYYDRYTCNELPSIPPDGGKYVAKLGNNIKGG
                     QVEQLRYLLTVNESNALFIYKYAVVFEDPGHKHDEQPFFDVAVRDNYGNIIDSLCGYN
                     HIISGGNIPGFNTCLIYDHPTDNTRDTVVRWKPWTTVAMDLTPYIGQTVMLEFTNADC
                     RLGGHWSYAYMEARTAAMKINVQACESDSIAILKAPEGFNSYVWSNGATTREIKVKLI
                     EGNTYSCVMTSIPGCSVTLQAKIIIERLTVTPPVSTICKGQSAELTASGADYYEWDKN
                     LGLGPVKTVFPLQTTTYSVTGITKNGCVARGTSTVTVLNPPQLTVQSNVPCEGETLRL
                     QAESNEQVSFEWRGPNAFESTKQYPEIPYSTVAMNGSYSVVATSTNGCKNSSTIQVTI
                     KPKPDITTSHTNACEGGEVSLRVFPSGLSYMWQGPGGFNSTNQFITRNPVTSNMTGVY
                     TVIGKNIYNCTDTASFSLQVYPSPIANFTHTNPCVGTAMKFTNLSTGATVYDWDFGNG
                     TYAYEAQPADVMYQSSVPYTVSLRVFNEFACSDTITKVVKPYAMPVAQFATDKACIGS
                     LYTIKNLSYVENDVLSSWEWNLGPKGTSTQLHPQIMLTDATPIPVTLTVRTQYCSNSQ
                     TKNILSYPKPVLSPFQSNGCAPLYVQFPSIAEERVFYVWDFGDGETSQSPNPAHYFFN
                     NTGLEKTFTVKQTVTTEFGCTDSTKHTINVYPQPISDFTVSKTAICSGESITLQNTSL
                     FANSYIWNLGNG"
     gene            2498..>2630
                     /locus_tag="P5243_05860"
     CDS             2498..>2630
                     /locus_tag="P5243_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRS19008.1"
                     /translation="MKVISTKKELQSEMEQIRKAGKTFGFVPTMGALHDGHLSLIHRS
                     "
BASE COUNT          814 a          490 c          467 g          859 t
ORIGIN      
        1 taccattacc taaattccat atatatgaat tagcaaataa agacgtattt tgtaatgtaa
       61 tagattcacc actgcatatg gcagtttttg aaaccgtaaa atcagatatt ggttgtggat
      121 acacgtttat tgtatgtttt gttgaatcgg tgcagccaaa ttctgttgtt accgtttgct
      181 taacagtaaa tgttttttct aatcctgtat tattaaagaa ataatgtgcg ggatttggtg
      241 attgcgaagt ctcgccatcg ccaaaatccc acacataaaa tactctttct tctgcaattg
      301 agggaaactg tacatatagg ggagcacagc cgttggattg aaaaggagat aaaacaggtt
      361 taggataaga aagaatattc ttggtttgag agttagaaca atattgggtt cgtacggtta
      421 atgtaacagg aataggagta gcatcggtta acataatttg aggatgcaat tgtgtagaag
      481 tacctttggg gcccaaattc cattcccatg atgataatac atcattttca acatacgaaa
      541 gattttttat agtatataaa ctgccaatac atgctttatc tgttgcaaat tgagcaacag
      601 gcattgcata tggttttaca acttttgtaa ttgtatcgga acacgcaaat tcgttgaaca
      661 ctcgtaaact tacagtatag ggaaccgaag attgatacat cacgtctgca ggttgtgctt
      721 cgtacgcata tgttccgtta ccaaaatccc agtcatacac agttgctccg gtggacaaat
      781 ttgtaaattt catagcagtt cctacacatg gatttgtatg tgtaaaattt gcaatcggtg
      841 aaggatacac ctgcaacgaa aaagatgcag tatcggtaca attatatata tttttaccaa
      901 taacagtata aacccctgtc atattggaag taacaggatt tcgagtaata aattgattgg
      961 ttgaattaaa tcccccggga ccttgccaca tatacgaaag tcccgacgga aatacacgta
     1021 atgaaacttc accaccctca catgcatttg tatgcgatgt tgtaatatcg ggttttggtt
     1081 taattgttac ctgaatagtt gacgaatttt tacatccatt tgtacttgtt gcaacaacag
     1141 aatatgaacc attcatggct acagtgctat agggtatttc cggatattgt tttgttgatt
     1201 caaaagcatt aggccccctc cattcaaaac tcacttgttc attactttct gcttgtaatc
     1261 taagagtttc tccttcgcac ggtacatttg attgaactgt taattgtgga ggattcaaca
     1321 ctgttacagt agatgtacct ctagcaacac agccattttt tgttattcct gttaccgaat
     1381 acgttgtagt ttgtaaaggg aacaccgttt ttaccggtcc taatcctaga tttttatccc
     1441 attcataata atcggcaccg gaagcagtaa gctcggcact ttgtccttta caaattgtag
     1501 aaacgggagg tgttactgta agtcgttcaa taattatttt tgcttgtaat gttaccgaac
     1561 aaccgggtat acttgtcata acacaagaat atgtatttcc ttcaattaat tttactttaa
     1621 tttctcgtgt agttgctcca ttgctccata catatgaatt aaaaccttcg ggagctttca
     1681 gtatagcaat tgaatccgat tcgcaagcct gtacatttat tttcatagct gctgtacgtg
     1741 cttccatgta agcataactc caatgtccgc caagtctaca gtcagcatta gtaaattcaa
     1801 gcatcaccgt ttgtcctata tatggtgtta aatccatagc tactgtagtc catggtttcc
     1861 atcgcactac ggtatcacga gtattatcgg tagggtgatc atatattaaa catgtattaa
     1921 atccgggaat atttcctcct gaaattatat gattatatcc acacaatgaa tctattatat
     1981 ttccataatt atcgcgtacc gcaacatcaa aaaacggttg ctcatcgtgt ttatgtccgg
     2041 gatcttcaaa aactaccgca tatttataaa taaacaaggc attcgattca tttaccgtca
     2101 ataaataccg taattgttct acttgtccac cttttatatt atttcccaat ttagcaacgt
     2161 atttacctcc atcaggcggt atactcggca attcattgca cgtatatcta tcgtaatata
     2221 ggtttgaaat aattgtttgt cttcctgtgg ttatggtatt attggtaaat gttataactc
     2281 ccgttgtgcg attccatgaa ccgttagatg ctgtccatcc ggtaaaattt cccaattcaa
     2341 aatcagagtt gagtgacgat gtttgtgaat acaatacatt ctcaaacaaa gcaaacaaaa
     2401 caattgttac cacatataca ctctgtttca taatcggaat tttacctcaa cttatacaaa
     2461 gtacgtatat gtgccaataa aaaaatggta aaaacgtatg aaagttatct caacaaaaaa
     2521 ggagcttcaa agtgaaatgg agcaaatcag aaaagcagga aaaactttcg gatttgttcc
     2581 aaccatggga gcattgcacg atggtcattt gtctttgatc catcgtagca
//
LOCUS       DAOTOW010000282         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30497_length_2629_cov_2.787102, whole genome shotgun sequence.
ACCESSION   DAOTOW010000282 DAOTOW010000000
VERSION     DAOTOW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30497_length_2629_cov_2.787102"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="P5243_05865"
     CDS             complement(<1..392)
                     /locus_tag="P5243_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRS19009.1"
                     /translation="MNFRHYIFCIVLVNLFTLHSAFSQENISFTTPQKYIIGGITVDG
                     VTYLQHPPIIRITGLSVGQKITVPGPDITNAVDRLWKQGLFGDIQIYASNIESDTIFL
                     TIYLQERARLNTITIEGVKKRKQSELIE"
     gene            complement(403..1149)
                     /locus_tag="P5243_05870"
     CDS             complement(403..1149)
                     /locus_tag="P5243_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRS19010.1"
                     /translation="MKFLQKKEIEKDRLPQHIAIIMDGNGRWAKKRGNIRLFGHQKGV
                     STVNEIVTASAEIGLRYLTLYAFSTENWNRPKDEVSGLMNLLVNALEKNLEKLLKNNV
                     RLLTIGNLESLPIEIQHKIYDAQELSKNNTGLTLILALSYSSRWEITEAVKNIAQKCI
                     EQKISIADIDYTTINSHLCTNNIPDPELVIRTSGEYRISNFLLLQSAYAEYYFTKTLW
                     PDFSKQEYIQAIVDFQHRERRFGKTGEQIK"
     gene            complement(1112..1849)
                     /locus_tag="P5243_05875"
     CDS             complement(1112..1849)
                     /locus_tag="P5243_05875"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRS19011.1"
                     /translation="MIVTTKLSTVLLCITCLFYSTTVVSQIYRSRDIGLFVGTSQYNG
                     DVNMTKPYYSPHWTAALHLRKTYNYRYSWRVSISYGELKGYDTDFLNKYQQHRKHDFT
                     KTHIYEMASLVEFNFFDITPDEKEHNFSPVLIGGAGIFYSEELKLKEMICFPVGLGLK
                     YKLAPNLELMAEWTFRKTYTDKLDQLNADALGYKRYKQISFNKTNDWYSLLGATLLIN
                     FMSDSAPCHIYDKKGYEIFTKKRNRKR"
     gene            complement(1951..>2629)
                     /locus_tag="P5243_05880"
     CDS             complement(1951..>2629)
                     /locus_tag="P5243_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19012.1"
                     /translation="FGMINGSDKQTQEFFRSDRDLSFRSPIYEFNVRLEYKIRKERAG
                     HIYDLKGVRGERPIKLVTYAFAGVGVFGFNPKAKIEGQWVALQPIGTEGQNFLPTRKP
                     YKRVQLCIPVGIQIKYLANRYWSYSFEVGPRFTFTDYIDDVSKTYVNPDLVAANDKTV
                     PDHIARRLADPKYGTERRIANDGVSYSYFNEQRGDPYDKDFYMFAMISVHYKLKATRK
                     GWPTFQK"
BASE COUNT          803 a          425 c          406 g          995 t
ORIGIN      
        1 aattcaatta attcagattg ttttcgtttt tttacacctt caatggttat agtgtttaat
       61 cgagctcgtt cttgtaaata tattgtaaga aatatagtgt ctgattcaat gtttgaagca
      121 tatatttgta tgtcgccaaa taatccttgt ttccataacc tgtctacagc gttggtaata
      181 tctggcccgg gaacagtaat tttttgtcct acagaaaggc ctgtaattct aattatgggt
      241 gggtgttgca ggtaagttac gccatcaact gtgatacctc caataatata tttttgcggt
      301 gttgtaaacg aaatattttc ttgagaaaat gcactatgta atgtgaaaag atttactaat
      361 actatacaaa aaatataatg tctgaaattc ataatgaatg tgttatttta tttgttctcc
      421 cgttttccca aatctgcgtt ctctgtgttg aaaatcaaca attgcctgaa tatattcttg
      481 ttttgaaaaa tcaggccaca atgtttttgt aaaataatac tcggcatatg cactttgtag
      541 caacaaaaaa ttgctgattc tatactctcc gcttgtacga ataactaatt cgggatcggg
      601 aatattgttt gtacaaagat gcgaatttat tgtggtataa tctatatctg caatggaaat
      661 tttttgttca atacattttt gtgcaatatt ttttactgct tcagtaattt cccatcgtga
      721 actgtaactt aatgcgagaa ttaatgtaag tcctgtatta ttttttgata attcttgtgc
      781 atcgtaaatt ttatgttgaa tttcaatggg taaactttct aagttcccta ttgtaagtag
      841 tcgtacattg ttttttagta atttttctaa atttttttct aatgcattta ctaataaatt
      901 cataagaccg gatacttcgt ctttcggacg attccaattt tcggttgaaa atgcgtataa
      961 tgttaggtat cgtaatccaa tttcagcaga tgctgttaca atttcattta cggttgatac
     1021 gcctttttga tgaccgaaca atcgaatatt gccgcgtttt tttgcccaac gaccattacc
     1081 atccattatt attgctatgt gttgcggcag tctatctttt tctatttctt ttttttgtaa
     1141 aaatttcata acctttttta tcgtatatat gacaaggagc actatcgctc ataaaattta
     1201 taagtaaagt tgcacccaat agagaatacc aatcatttgt tttattaaat gatatttgtt
     1261 tgtatcgttt atatcctaaa gcatcggcat ttaattggtc taatttgtct gtatatgttt
     1321 ttctaaaagt ccattctgcc ataagttcaa ggttaggtgc taatttgtat ttaagcccta
     1381 aaccaaccgg gaaacatatc atttctttta attttaattc ttcggaataa aaaattcctg
     1441 caccaccaat taaaaccggt gaaaaattat gttctttttc atcaggagta atatcaaaaa
     1501 aattaaattc aacaagtgaa gccatttcat atatatgtgt ttttgtaaaa tcatgcttcc
     1561 ggtgttgttg atatttgttt aaaaaatctg tatcatatcc ttttaattca ccatatgaga
     1621 tagatactct ccaagaatat ctataattat atgtttttcg aagatgtaat gcagctgtcc
     1681 aatgtggtga ataataaggc tttgtcatat ttacatcgcc attgtattgt gaagttccta
     1741 caaataaacc tatgtctctg cttctatata tttgcgatac caccgttgtg ctgtaaaaca
     1801 agcaagtaat gcataataaa acagttgata atttggtggt tacaatcata aaggtacttt
     1861 aatacatatt ttgatgcaaa aatactattt tgcttcaata atgaattata tactaacgat
     1921 ataataataa aaaaagtatg attgaaccaa ttatttttgg aaagtaggcc aacctttacg
     1981 agttgcttta agtttgtaat gaaccgaaat catggcaaac atataaaaat ctttgtcata
     2041 cgggtcgcct cgttgttcat taaagtatga ataacttaca ccatcattag ctattcttcg
     2101 ttcagttccg tatttggggt cggctaatct acgggctata tggtcgggta cggttttgtc
     2161 gtttgctgca actaagtcag gatttacata tgttttactt acatcatcta tatagtcggt
     2221 aaatgtaaag cgagggccaa cttcaaaaga gtaactccaa tatcggttag ctaaatattt
     2281 tatttgtatt ccaacaggta tacataattg aactcttttg taaggtttac gagttggtaa
     2341 aaaattttgt ccttctgttc caattggttg taacgctacc cattgccctt caatttttgc
     2401 ttttggatta aatccaaata ctccaacacc tgcaaatgca tatgttacta attttattgg
     2461 acgttcaccg cgtacacctt ttaagtcata tatatgtccg gcacgttctt ttctaatttt
     2521 atattctaat cgaacattaa attcataaat aggactacga aacgataaat ctctatcact
     2581 tctgaaaaat tcttgtgttt gtttatcgct accgtttatc ataccaaaa
//
LOCUS       DAOTOW010000283         2623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30596_length_2623_cov_3.565421, whole genome shotgun sequence.
ACCESSION   DAOTOW010000283 DAOTOW010000000
VERSION     DAOTOW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30596_length_2623_cov_3.565421"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /gene="mraY"
                     /locus_tag="P5243_05885"
     CDS             <1..1208
                     /gene="mraY"
                     /locus_tag="P5243_05885"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRS19013.1"
                     /translation="PGAGVFEFLTFRAAGAFITSLIISIIFGKRIIQFLQKLQIGEVI
                     RDLGLEGQMQKKGTPTMGGILIISAIIIPTLLFAKLTNVYILLLLFTTVWFGFLGFAD
                     DYIKVFKKNKEGLSGKLKIVGQVILGLCIGLTLYLHPDVVIVQKSTSIVSATEYTSSY
                     SNENIEQTRAEKYGEKSTKTTIPFFKNNEFDYKYLVGFLGNYAQPIGWIVFVIFVILI
                     TTSVSNGVNLTDGLDGLATGTTAISGVVLGILAYLSGNIIYADYLNIMYIPHSGEMVV
                     FASAFIGATIGFLWYNTYPAQVFMGDTGSLTLGGIIAVFAILIRKELLIPILCGVFLM
                     ENLSVVLQVSYFKYTKKKYGEGRRIFLMSPLHHHYQKKGIPESRIVARFWIVSLLLAA
                     FTLLTLKLR"
     gene            1214..2563
                     /gene="murD"
                     /locus_tag="P5243_05890"
     CDS             1214..2563
                     /gene="murD"
                     /locus_tag="P5243_05890"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRS19014.1"
                     /translation="MIHNNTIVVLGAGESGTGAAILAKKQGFYVFVSDAGTISATYKQ
                     LLDDNAIPYEEGTHTTDIIFQAKEIIKSPGIPEKAPLIQQVKSLGIPVISELEFAARY
                     TNAKLICITGSNGKTTTTLLTYHILKQAGLHVGLAGNVGKSFALQVANETYDYYVIEI
                     SSFQLDDMYDFKADIAVLLNITPDHLDRYEYTFQNYINSKFRILQNMNSHDSFIYCND
                     DEVLIRELQQRTIVPHIFGYTTTNDSSQTGYLDSNTIYVKTPQKENLSMSVYDMLLKG
                     KHNYGNTMAAAIAAQIVDIKNEQIRESLRTFSGVEHRLEKLSYTVRGVEFINDSKATN
                     VNSTWYALESMDDNVIWIAGGTDKGNEYDSLYELATAKVKALVCLGVDNTKLIESFTG
                     KIPVITEAKSMKEAVTKAYKLAQKGDTVLLSPCCASFDLFKNYIDRGLQFKQEVRNL"
BASE COUNT          921 a          355 c          478 g          869 t
ORIGIN      
        1 taccgggagc cggagtgttt gaattcttaa cttttagagc tgcaggtgca tttattacgt
       61 cattaattat ttcaataatt tttggtaaac gaattattca atttttgcaa aaattacaaa
      121 taggtgaggt tattcgtgat ttgggtctcg aaggtcaaat gcagaaaaaa gggactccta
      181 ctatgggtgg tatattaata atttcagcga ttataattcc aacactttta tttgcaaaac
      241 ttacaaatgt atatatactg ttgttgcttt ttactacagt gtggtttgga tttttaggtt
      301 ttgctgatga ttatattaag gtatttaaaa agaataaaga aggcctttca ggaaaactta
      361 aaattgtagg gcaggtaatt ctcggtttgt gtatagggct tacattgtat ttgcatcccg
      421 atgtagttat agtacaaaaa tcaacatcta tagtttctgc tacggaatat accagtagtt
      481 acagtaatga aaatattgaa caaacacgtg cagaaaaata tggtgaaaaa tcaactaaaa
      541 ctactattcc attttttaag aataatgaat ttgactataa atatttagtt ggatttttag
      601 gaaattatgc tcaaccaata ggatggattg tatttgtaat ttttgtaatt cttattacta
      661 caagtgtttc gaatggtgta aatttaactg atggacttga cggtcttgca acaggaacta
      721 cagctatatc tggtgtagtt ttaggtattt tggcatattt atcagggaat attatttatg
      781 ccgattattt aaatataatg tatatacctc attccggcga aatggtggtt tttgccagtg
      841 cttttattgg agcaactatt ggttttttat ggtacaatac ataccccgct caagttttta
      901 tgggcgatac cggaagctta actttggggg gcataatagc tgtttttgca atacttatcc
      961 gaaaagaatt acttattcca atattatgtg gtgttttttt aatggaaaat ttgtctgttg
     1021 ttctacaggt aagttatttt aaatatacca agaaaaaata tggtgaaggt cgcagaatat
     1081 ttttaatgtc acctttgcat catcattatc agaaaaaagg aatacccgaa tcacgaattg
     1141 tagcaagatt ttggatagtt tcattgctgc ttgctgcatt tacattatta accctaaaac
     1201 tacgataaaa ccaatgatac ataataatac tatagtagtt ttaggagcag gtgagagtgg
     1261 tacaggagct gccatacttg ccaaaaagca aggattttat gtgtttgttt cagatgcagg
     1321 tactatttct gctacatata aacaattact cgatgataat gctattcctt atgaagaagg
     1381 tacgcatact accgatatta tatttcaagc aaaagaaatt attaaaagtc ccggaattcc
     1441 cgaaaaagct cctttgatac aacaagtaaa atcattgggt ataccggtaa tttccgaatt
     1501 ggaatttgca gcacgatata caaatgcaaa attaatttgt attacaggta gtaatggaaa
     1561 aactactaca accctgctta catatcatat acttaaacaa gcaggtttac atgttggctt
     1621 agcaggaaat gtaggtaaaa gttttgctct gcaagtagca aatgaaacat atgattatta
     1681 tgtgattgaa attagtagtt ttcaactcga tgatatgtat gacttcaaag cagatattgc
     1741 tgtactactt aatataactc cagaccattt agatagatat gaatatacgt ttcagaatta
     1801 tataaattct aaatttagaa ttctgcaaaa tatgaattct catgatagct ttatttattg
     1861 taatgatgac gaagtactta tacgtgaatt acaacagcgc actatagtgc cgcatatttt
     1921 tggctatact actaccaatg attcttcaca aacaggatat ttagattcaa atacaatata
     1981 tgtaaaaacc ccacaaaaag aaaatttaag tatgagtgta tacgacatgt tactcaaggg
     2041 caaacacaac tatggtaata caatggcagc agctattgca gctcaaatag ttgatattaa
     2101 aaatgaacaa attagagaaa gtttgagaac atttagcgga gttgaacata ggttagaaaa
     2161 gttaagctat acggtgcgtg gagtggaatt tataaatgat tctaaagcta ctaatgtaaa
     2221 ctctacttgg tatgcattag aaagtatgga tgataacgta atatggattg caggaggaac
     2281 agataaaggg aatgaatatg attctttata cgaattggca actgctaaag taaaagcact
     2341 tgtttgttta ggcgttgata acacaaaact tattgaatct tttaccggca aaattcctgt
     2401 aataacagaa gctaaaagta tgaaagaagc agttacaaaa gcatataaac ttgctcaaaa
     2461 aggtgataca gtattgcttt ctccctgttg tgcaagtttt gacttattta aaaattatat
     2521 tgacagagga ttacaattta aacaagaagt aagaaactta tgatgtatag aggaattata
     2581 gaatttttca aaagcaaatt taaaggcgat aaggtaatat ggc
//
LOCUS       DAOTOW010000284         2611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30765_length_2611_cov_2.605243, whole genome shotgun sequence.
ACCESSION   DAOTOW010000284 DAOTOW010000000
VERSION     DAOTOW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30765_length_2611_cov_2.605243"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2611
                     /gene="sprA"
                     /locus_tag="P5243_05895"
     CDS             <1..>2611
                     /gene="sprA"
                     /locus_tag="P5243_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRS19015.1"
                     /translation="GNSTNYPDNEDLNNDNTLQETEAYYQYHISLRPEDMVIGKNFIV
                     DKIVETTKQNNRTTYTNWYQIKIPIQTEDKQRIGDISDFRDIQTMRMFLRGFSDSVVL
                     RIAEFALVYSSWRRYNKPLYEAGEYDLFNDSEFDISVVNIEENASRSPINYVLPPGVD
                     RVIDPNSQSIKELNETALSLKVVDLEDGNSKAVYKQFNKDLRQFGKIEMFIHAEALED
                     PAYNLEDGDLCAFIRIGSDFTENYYEYEVPLSLTLPGKYNKDIESDRLKVWPQENMVS
                     LDLATLKNLKLERDKMIADGVLSDLQSYHVYSIAQGKNRVSIKGKPNLGNVRTIMIGV
                     RNKKKSVKNPFDDGLKKSGIVWFNEFRLTDIDDQSGWAAIASARIAFADFATLSLSGK
                     TSKPGFGGIDQKIFERSQEDLYQYDIASNVALHKFFPEAWSISLPFYVDFSEIYIMPQ
                     YDPTNPDILLKQSLENMTTQHERDSLLRITRDFTQRFSYNFTNVRINKQSTKQYPWNI
                     SNFSTSFAFNRFYSYDIDYEKNYNHQYKASFNYMYNLRPKNYTPFKSNKNAFIKDINF
                     FLLPTTVSIKNEWDRTYKEQQRRNLSQYGGSLPLTASKKFLWLRSYDVNYALSKNLKF
                     TYSALNQSRINEPYGVIDKTNEEVWQDYRREVYRNMQTYGENMSFSQKTTVSYQLPFN
                     KIKPLDWITSNYKYSGSYDWQLGAEIRNQDNFGNTITNSNTQSLDNTFNFTRLYNKSK
                     YLNEVNKRMRNRGTNQPKKKETVKYNQENLKLKANVPIVINHKLKTEDVRVSMLDDKG
                     QVVMGTTKVLSPTQVEFTPNKDVDNAKVTVTGKRDVVETQFTKIMDQVVTVLMMTKTL
                     SVNYTQSEGTYIPG"
BASE COUNT          999 a          353 c          434 g          825 t
ORIGIN      
        1 ggcaattcaa cgaattatcc tgataatgag gatttgaata atgataatac tttgcaagaa
       61 acagaagctt attatcaata tcatataagt ttacgccccg aagatatggt tataggaaag
      121 aattttatag ttgataaaat tgttgaaact actaagcaaa ataatagaac aacatataca
      181 aattggtatc aaataaaaat acctatacaa accgaagata aacaaagaat aggcgatatt
      241 tctgatttta gagatattca aactatgaga atgtttttac gaggttttag tgattctgtt
      301 gtattaagaa tagcagaatt tgctttagta tatagtagtt ggcgtagata caataaacca
      361 ttatacgaag ccggagaata cgatttattt aatgattctg aatttgatat ttctgttgtt
      421 aatattgaag aaaatgcatc gcgttctcct ataaattatg tgctgcctcc cggtgttgat
      481 agagttattg atcctaatag tcaaagtatt aaagaattaa atgaaacagc attgtcgctt
      541 aaagttgttg atttagaaga tggtaattca aaagctgtat ataaacaatt taataaagat
      601 ttgcgccaat ttggtaaaat tgaaatgttt atacatgccg aagcattaga agatcctgct
      661 tataatttag aagatggtga tttatgtgct tttattcgaa taggttctga ctttaccgaa
      721 aattattatg aatatgaagt tcctttgagt ttaactttac cgggaaaata taataaagat
      781 atagaatcgg atagacttaa agtttggcca caagaaaata tggtctcgct tgatttagct
      841 actcttaaaa atctgaaatt ggaacgagat aaaatgatag ctgatggtgt tttgtctgac
      901 ctgcaatcgt atcatgtata ttctatagcc cagggtaaaa atagggtaag cataaaagga
      961 aaaccaaatt taggtaatgt tcgcactata atgataggtg tacgtaataa gaaaaaatcg
     1021 gtaaaaaatc cgttcgatga tggattaaaa aaatctggaa ttgtatggtt taatgagttt
     1081 agattgacag atattgatga tcaaagtggt tgggcagcaa tagcttctgc tcgtatagca
     1141 ttcgccgatt ttgctacatt atctttatcc gggaaaacaa gtaaacccgg gtttggaggt
     1201 atagaccaaa aaatatttga acgaagtcag gaagatttgt atcaatacga tatagcgtct
     1261 aatgttgctt tacacaaatt tttcccggaa gcttggagta ttagcttacc tttttatgta
     1321 gatttttccg aaatatatat tatgccacag tatgacccta ccaatcctga tattttgttg
     1381 aaacaatcac ttgaaaatat gacaacgcaa cacgaacgag attcattact tagaattact
     1441 cgtgatttta cacagcggtt ttcatataat tttactaatg ttcgtataaa caaacaatca
     1501 acaaaacaat acccttggaa tatttctaac ttctctacaa gttttgcttt taatcggttt
     1561 tattcgtatg atattgatta tgaaaaaaat tataatcacc aatataaagc ttcatttaat
     1621 tatatgtata atttacgtcc taaaaattat acaccgttta aatcaaataa aaatgcattt
     1681 ataaaagata ttaatttctt tttgttgcca acaactgtta gtattaaaaa tgaatgggac
     1741 agaacatata aagaacaaca aagacgaaat ttatcacaat atggaggaag tttgcctctt
     1801 acggcaagca aaaaattctt atggttacgt tcgtatgatg taaattatgc attatctaaa
     1861 aatcttaaat ttacatattc tgctcttaat cagtcacgta taaatgaacc atatggcgta
     1921 attgataaaa cgaacgaaga ggtttggcaa gattatagac gagaagtata cagaaatatg
     1981 caaacatatg gtgaaaatat gtcgttttca caaaagacta ctgtttctta ccaattacca
     2041 ttcaataaaa ttaaaccttt agactggatt acctcaaatt ataagtattc gggttcatat
     2101 gattggcaat taggtgcgga aattcgtaat caagataatt ttggaaatac tattacaaat
     2161 tcaaatacac aaagtcttga taatacattt aattttactc gattgtataa taaatctaag
     2221 tatttgaatg aagtaaataa acgaatgcga aatagaggaa caaatcaacc caagaaaaaa
     2281 gaaactgtaa aatataatca agaaaatcta aaattaaagg ctaacgtacc aatagttata
     2341 aatcataaat taaaaacaga agatgttaga gttagcatgc tcgatgataa aggccaagtt
     2401 gtaatgggaa ctacaaaagt actttcgcct acacaagttg aatttacacc caataaagat
     2461 gtagataatg caaaagtaac agtaaccggt aaacgtgatg tggttgaaac ccaatttact
     2521 aaaattatgg atcaagttgt aactgtatta atgatgacaa aaacattgtc agtaaattat
     2581 actcaatcag aaggaacata tatacccgga t
//
LOCUS       DAOTOW010000285         2600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30948_length_2600_cov_3.293124, whole genome shotgun sequence.
ACCESSION   DAOTOW010000285 DAOTOW010000000
VERSION     DAOTOW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30948_length_2600_cov_3.293124"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2460)
                     /locus_tag="P5243_05900"
     CDS             complement(<1..2460)
                     /locus_tag="P5243_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19016.1"
                     /translation="MKLKTLFWILFISITFTCNAQYFLWGTDPASIRWEQIQTNRFQV
                     IYPSGQDSIAQRFTNLLEYVYDAAGKTLGHAPQKISVIIHTSSTISNGSVAWAPKRMD
                     IFDVSPQDQTSMEWLENLAIHEYRHVVQYDKLNQGLTKILYFLLGEQAPAAIGGIFLP
                     SWFFEGDAVVTETALSKSGRGRQPEFTMDVRAQLIEKKKYSYAKAYFGSYKDHVPNHY
                     KMGYVVVANSRDIYGPQLWNNTVTNVARRPYQIMPFNKAIKIQTSYNKRKLYHEMFNK
                     QLIQWNLEYNREIHKSYDTLTKRNPKEYTNYLYPQRISDTYVIAEKQGLGDASKIVLI
                     SKEGIEKTIANTSFKQPNERIHTNGELVVWAETNPHIRWELQGSSDIFIYSLKTNKTE
                     RFKTKLRVFAPCISPLGDKIATVLIDSTSKYSIVVYNTATKQMIAQYPSPGQEFIQNP
                     SWNFDASKIVYIGLSSKGKRLVELNMKNGERTVLIDYTNDDIQNPMYWNNFVIYSSSY
                     SGVDNLYAIQTQTREILRITVAKFGARYVSAYHNVLLYSDYTSDGYMIAQIPADIRSW
                     HTIDVVRKAKYELAESISKQEPITVNFETKQDSIYQTKPYSKLLHAVNIHSWMPFYLE
                     YNANQSNEAGNGFQILSQDKLSSTIISAGYRKNYTNSRRAGFVKVTYTGIFPIIDLDY
                     QNGYQEFTKIIDNDTIKILYHLSQIVTGIYIPLNFSSRSYYRYLILSSQIQYGTYSKT
                     SINIEAYDSYFKNFFDVGIVTYSASFSNILQTSTRDIMPRIGQVFTINFAHAPLNASE
                     IYHENIFGSTKIYLPGFYKHHS"
BASE COUNT          816 a          389 c          432 g          963 t
ORIGIN      
        1 actatgatgt ttatagaatc cgggcagata tatttttgtg gaaccaaata tattttcatg
       61 atatatttca cttgcattga gaggagcatg agcaaaatta attgtaaata cttgtcctat
      121 tcgtggcatt atgtcgcgag tgcttgtttg aagtatatta ctaaatgaag cagaatatgt
      181 tacaatacct acatcaaaga aatttttgaa atacgaatca tatgcttcaa tatttatact
      241 tgtttttgaa tacgtgccat attgtatttg actactcaga ataaggtacc tatagtacga
      301 acgtgaagaa aaattaagtg gtatataaat tccggtaact atttgactga gatgatataa
      361 tattttaata gtgtcattat caataatttt tgtaaattct tgatatccat tttgatagtc
      421 taaatctata attggaaata taccggtata tgtaacttta acaaatcctg cacgtcgaga
      481 attggtataa ttttttctat atcctgccga aataatggta gagcttaatt tgtcttgaga
      541 aagtatttga aatccattac ctgcctcatt cgattgattt gcattatatt ctaagtaaaa
      601 aggcatccaa ctgtgtatat ttacagcatg tagtaatttt gaatatggtt ttgtttgata
      661 tatgctatct tgtttagttt caaaatttac agtaataggt tcttgtttcg aaattgattc
      721 tgcaagttca tattttgctt tacgaacaac atcaatagta tgccaacttc ttatatcggc
      781 aggtatttga gctatcatat atccatctga agtataatca gaatatagta gcacattatg
      841 gtaggccgaa acatatcgtg ctccaaattt tgcaacagta attctaagta tttcacgagt
      901 ttgggtttga attgcatata aattatcaac acctgaatat gaagatgaat atattacaaa
      961 attattccaa tacatagggt tttgaatgtc atcatttgta taatctataa gtacagttcg
     1021 ctctccattt ttcatgttta attcaacaag tcgtttgccc ttagaagata atcctatata
     1081 cactattttg cttgcatcaa agttccaaga agggttttgt atgaattctt gaccgggaga
     1141 aggatattga gctatcattt gtttggtagc ggtgttatat actactatag aatatttgct
     1201 ggtagaatct ataagaactg ttgcaatttt gtcgcccaag ggtgaaatgc acggagcaaa
     1261 aacacgtaat tttgttttga aacgttctgt tttatttgtt tttaaagaat aaataaaaat
     1321 atctgaagag ccttgtaatt cccaacgaat atgaggattt gtttcagccc atactactaa
     1381 ttccccattg gtatgtattc gttcatttgg ttgcttaaac gaggtgtttg ctatagtttt
     1441 ttcgatacct tcttttgata ttaatacaat ttttgaagca tcacccaagc cttgtttttc
     1501 tgctattaca tatgtatcag aaatgcgttg aggatacaag tagttagtat attcttttgg
     1561 atttcgttta gtaagtgtat cataagattt atgaatttcg cgattatatt ctaaattcca
     1621 ttgaatcaat tgtttgttaa acatttcatg atacagtttg cgtttattat acgaagtttg
     1681 aatttttata gctttgttga aaggcattat ttgataaggt ctgcgagcaa catttgttac
     1741 agtgttgttc cataattgag gtccatatat atcacgagaa ttagctacaa ccacatatcc
     1801 cattttatag tgattaggaa cgtgatcttt gtaagaaccg aaatacgctt ttgcgtatga
     1861 atattttttt ttctctatta attgagctct tacatccatt gtaaattctg gttgacgacc
     1921 acgacccgat ttgcttaacg ctgtttctgt tacaacagca tcaccttcaa aaaaccacga
     1981 aggtaaaaat atgccaccta ttgctgccgg agcttgttcg ccgagtaaga aatataaaat
     2041 ttttgttaat ccctgattaa gtttatcata ttgaaccaca tgtctatatt cgtgaattgc
     2101 aagattttca agccattcca tacttgtttg gtcttgtggt gatacgtcaa atatatccat
     2161 acgtttggga gcccatgcta ccgacccatt tgaaatagta gatgatgtgt gtatgattac
     2221 tgaaattttt tgtggtgcat gtcccaatgt ttttcctgcg gcatcataca catattcaag
     2281 taaattggta aatcgttgag caatactatc ttgtccactt ggatatataa cttgaaatct
     2341 gtttgtttgt atttgttccc aacgtattga tgccggatct gttccccaca aaaaatattg
     2401 agcgttacaa gtaaatgtaa tgcttataaa gagtatccaa aaaagagttt ttagtttcat
     2461 gtattatgaa attaatttga gtgcaagttc aaaaaaaact atgattatac caaaaaaata
     2521 tatattttgc ataatatttt tattcataac tgtcaagttt cacaaggttg tttatgataa
     2581 cttaattgca aagataaaac
//
LOCUS       DAOTOW010000286         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_30989_length_2597_cov_4.419748, whole genome shotgun sequence.
ACCESSION   DAOTOW010000286 DAOTOW010000000
VERSION     DAOTOW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30989_length_2597_cov_4.419748"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="P5243_05905"
     CDS             <1..683
                     /locus_tag="P5243_05905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS19017.1"
                     /translation="ITNIIANSEATKHSITAYNSNLFPLDKITVLYNGIPQCNIDKKY
                     DTGNKLYITIGTAGRLEPQKNMNAVLTIAQEVLKNTTNFVIRIAGDGSLYQTLKQAIQ
                     DKRLQSHIELVGFQNNINDFYATLDIFILTSHGEGFGYVMAEAMRYSIPVIAYNVSSS
                     QELITDNYNGFLIPKDDTIQFAQKIVYLIENEQERQKMGANAREFVQSHFDAQNIFEK
                     TEAFITQL"
     gene            696..1463
                     /locus_tag="P5243_05910"
     CDS             696..1463
                     /locus_tag="P5243_05910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS19018.1"
                     /translation="MHKLSVAIITFNEEKNIERCIRSVQDIADEIVVVDSYSTDATVE
                     ICKKLNVSCILHPFEGHIQQKNYARMLCKYDLVLSLDADEALSDTLRASIQTIKQNHT
                     ADGYTCNRLNHFCGTPVHHCGWYPDISLRLWNRNKGEWGGNNPHDKFIMHPNTTIIHL
                     QGDILHYTVHTVEQAIAQINSFSTISAQSKYSQGKSSSITKIVIYPLWRFIRNYILKL
                     GFLDGLTGFIISKNAAYAVYLKYLKLYYMQKTNSNKQ"
     gene            1470..2456
                     /locus_tag="P5243_05915"
     CDS             1470..2456
                     /locus_tag="P5243_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS19019.1"
                     /translation="MKILYINSTHSDYLQDILYSGLVKILGTRSVKTVPWNKQYLLPV
                     KQYPKNLGYQSGETLQYILSCILPFRYDCVIVAAAKPDAFKTYIQIAPSIPSHIPVIF
                     IDGGDFSNVGGDLERKNCYELYEQALAIREFNIIFKREKLIDTYYPSNVFACPFAFNL
                     DTIKQIGSVSKTYDVSFWAVESDPIRTQALTLIADLFDCAQNGTTLNQTFKQYKRKGL
                     FYLEELKRCKIVLNFRGRGWDTLRYWEVPALGVCMISQKPQIEIPNPFTHGANIIYCN
                     DDLSNLIELCEYYLKHNDKREQIAQQSYNHIAAYHTDVKRATYVLSIIQKHV"
BASE COUNT          966 a          445 c          394 g          792 t
ORIGIN      
        1 ttattactaa tattattgcc aattctgaag ctaccaaaca ttcaattaca gcatacaatt
       61 caaacttatt tccactcgat aaaattaccg tattgtataa tggtatacca caatgtaata
      121 ttgataaaaa atatgatact ggtaacaaat tatatattac aataggtact gcaggaagac
      181 ttgagccaca aaaaaatatg aatgctgttc ttacaattgc tcaagaagta ttgaaaaata
      241 ctacaaactt tgttattcgt atagcaggtg atggcagttt gtatcaaact ctcaaacaag
      301 ccatacaaga caaacgacta caatcacata ttgaattagt tggttttcaa aataatatca
      361 acgattttta tgcaacatta gatatattca ttcttacatc gcatggagaa ggatttggat
      421 atgtaatggc agaagccatg cgttactcta ttccggtaat tgcctataat gttagcagtt
      481 cacaagaatt aattaccgat aattataacg gttttttaat tcccaaggat gacaccatac
      541 aatttgctca aaaaattgta tatttaattg aaaatgaaca agaaagacaa aaaatgggag
      601 caaatgctcg tgaatttgtt cagtctcatt ttgatgcaca aaatatattt gaaaaaacag
      661 aagcgtttat tacacaatta taatgcaaat ttgccatgca caaattatcg gtagcaatta
      721 taacatttaa cgaagaaaaa aacatagaac gatgcatacg ttcggtacaa gatattgccg
      781 acgaaatagt agtagttgat tcatactcaa cagatgcaac tgttgaaata tgcaaaaaat
      841 taaatgtatc gtgcatattg catccgtttg aaggtcatat tcaacaaaag aattatgccc
      901 gaatgctttg taaatatgac ttagttttat cacttgatgc cgacgaagca ctctcagaca
      961 ccctgagagc cagcatacaa acaattaaac agaatcacac tgccgatgga tatacttgta
     1021 acagactcaa tcacttttgc ggcacccccg ttcatcattg cggttggtat ccagatataa
     1081 gtttacgact atggaatcga aacaaaggtg aatggggagg aaacaatcca catgacaaat
     1141 ttataatgca cccaaacact actatcattc atttacaagg cgatatattg cattataccg
     1201 tacacactgt agaacaagca atagctcaaa taaactcatt ttctacaatt tctgcacaat
     1261 caaaatattc acaaggaaaa tcatcatcta ttacaaaaat tgtaatttac ccactatggc
     1321 gtttcattag aaactatata cttaaattag gatttttaga tggacttacc ggttttatta
     1381 ttagcaaaaa tgcggcatat gcagtatatc ttaaatacct caaactttat tatatgcaaa
     1441 aaactaactc aaataagcaa tagttacaca tgaaaatttt atatattaat tcaacccatt
     1501 ctgactattt acaagatatt ttgtatagcg gactggtaaa aattcttgga accaggtcgg
     1561 taaaaacagt tccgtggaac aagcaatatt tattacctgt taaacaatat cccaaaaatc
     1621 ttggttatca atcgggagaa acgttgcaat atatactttc atgtattctt ccatttcggt
     1681 atgattgcgt aattgtagca gcagcaaaac ctgatgcatt taaaacatat atacaaattg
     1741 cgccttcaat cccctcgcac ataccggtaa tatttattga tggaggtgat tttagtaatg
     1801 ttggcggcga tttagaacgc aaaaactgtt atgaattata tgaacaagct cttgctatac
     1861 gtgaattcaa tatcattttc aaaagagaaa agctcattga cacatattat ccttcaaacg
     1921 tttttgcatg tccttttgca tttaatttag atacaatcaa acaaattggt tctgttagca
     1981 aaacgtatga tgtttctttt tgggctgtag aatcagaccc tattagaaca caagcactta
     2041 cactgattgc cgatttattt gattgtgcac aaaacggtac aacactcaat caaacattta
     2101 aacaatacaa acgcaaagga ttattctatt tagaagaatt gaaacgatgt aaaatagttt
     2161 tgaattttag aggtcgcggg tgggataccc ttagatattg ggaagttcct gcattgggag
     2221 tgtgtatgat ttctcaaaaa ccacaaattg aaataccaaa tccgtttacc cacggtgcaa
     2281 atattatata ttgcaatgac gatttatcaa atcttataga attatgtgaa tactatctga
     2341 aacataatga caaacgagaa caaatagcac aacaatcata taatcatata gcggcctacc
     2401 atactgatgt aaaacgtgct acatatgttc tttcaattat tcaaaaacat gtataaccgg
     2461 tagagtatga atatagtatt tacaggcaat tattcttctg aatataatag aactgcaatt
     2521 attattgaag gtcttaagca catacccgga atttcaataa tagaatatcc aattcaaaaa
     2581 aataacacat gtgatac
//
LOCUS       DAOTOW010000287         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31133_length_2589_cov_2.652328, whole genome shotgun sequence.
ACCESSION   DAOTOW010000287 DAOTOW010000000
VERSION     DAOTOW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31133_length_2589_cov_2.652328"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1034
                     /locus_tag="P5243_05920"
     CDS             72..1034
                     /locus_tag="P5243_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRS19020.1"
                     /translation="MPIVNYLVYSILHLAPQSHLGQSVHFFIYDTIKILILLFLISSL
                     MGIVNTYFPVDRLRLFLTTKKLYGFQYILASLFGAVTPFCSCSSIPLFIGFVKGGIPL
                     GVTFSYLITSPLVNEVAVAMFLGFFGIKATLVYAVSGILLGTIAGYILGLFNLERYLA
                     GWVQEMHKQAENEVEDWNENVVSFIDTLPTIIYDGWSIVKKVLLYILIGIALGAVMHG
                     YVPEDFFATYLSADSWFTVPLAVILAVPMYANAAGVVPVIQVFVAKGVPLGTALAFMM
                     AVVGLSLPEATLLKKVMTFKLLGIFFGTVTIAIILLGYLFNVIL"
     gene            1038..1268
                     /locus_tag="P5243_05925"
     CDS             1038..1268
                     /locus_tag="P5243_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19021.1"
                     /translation="MSQYYNGIISNLHMFVFICFLAFATLLSCTRECNCVKKVNGEVT
                     YTEVVKQRGSCKELNERAEIFDLVTEYTCEKK"
     gene            complement(1322..2212)
                     /gene="atpG"
                     /locus_tag="P5243_05930"
     CDS             complement(1322..2212)
                     /gene="atpG"
                     /locus_tag="P5243_05930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRS19022.1"
                     /translation="MAGIKEIRTRIASVNNTQKITSAMKMVSAAKLRKAQQAVQQMRP
                     YSEKLQEILTNVSADSDANQENIYGKARREERILIVAIASNGGLCGGFNSNIGKTTIS
                     LIQNEFAVQHAKKQVYIYAVGKKVRDMLKSKGFEPTKSFVDIYDNLSYENVAKLSETI
                     MHEFVSGTYDKVLLVYNSFKNAATQIITTKQFLPIEAPQTIALQSVKKFNYDYIFEPT
                     KEYIITELIPRSLKIQFYSAMLDSFASEHGARMTAMHKATDNAGELLKSLRIDYNKAR
                     QASITNEILEIVSGANALKG"
     gene            complement(2236..>2589)
                     /locus_tag="P5243_05935"
     CDS             complement(2236..>2589)
                     /locus_tag="P5243_05935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRS19023.1"
                     /translation="VDQAQFRELEAFSKFGSDLDAATMAVLDKGRRNVEILKQGQYSP
                     VPVQHQVAIIYCGTKGALQHVPIHKVREFEKDYIEYLELKHKDTLQKIAEGVFNDEIT
                     NVLDAATKEVSAKYK"
BASE COUNT          771 a          377 c          450 g          991 t
ORIGIN      
        1 gtaaaaggat tcaaacgatt agttcaatta ttataatagt acctttatta ataggaatat
       61 atatatattt aatacctatt gttaattatt tagtatatag tatattgcat ttagcaccac
      121 aaagtcattt aggtcaatcc gtacattttt ttatatatga tacaattaag attcttatat
      181 tattatttct tataagttct cttatgggaa ttgtgaatac atatttcccg gttgacagat
      241 tgcgattgtt tttaacaaca aaaaaactat atgggtttca atatatttta gcgtctttgt
      301 ttggggcagt aactcctttt tgttcgtgtt catctattcc cttatttatt ggttttgtaa
      361 aaggaggtat tccattggga gttacatttt cgtacctcat tacatctcct ttagtaaatg
      421 aggtagcggt ggcaatgttt ttgggttttt ttggaataaa agctaccttg gtgtatgctg
      481 taagtggtat attattgggc acaatagccg gctatatatt agggcttttc aatcttgaac
      541 ggtatttagc tggttgggta caagaaatgc acaaacaagc agaaaatgaa gttgaagatt
      601 ggaatgaaaa tgtagtttca ttcatagata cattaccaac aattatatat gatggttggt
      661 ctatagtaaa aaaagttctt ttatatatat taattggaat tgctttgggg gctgtaatgc
      721 acggttatgt tcccgaagat ttttttgcaa catatttgtc tgcagattct tggtttactg
      781 ttcctttagc ggtaattctt gctgttccta tgtatgctaa tgcagcaggt gttgttcctg
      841 tgattcaagt atttgttgct aaaggtgtgc cacttggtac tgcattagcc tttatgatgg
      901 cagttgttgg tctttcatta cccgaagcta cattgttaaa aaaagtaatg acttttaagt
      961 tgttaggtat tttttttgga actgttacta ttgcaattat attgttgggg tatttattta
     1021 atgttatttt ataaaatatg agtcagtatt acaatggtat aataagtaat cttcatatgt
     1081 ttgttttcat ttgtttttta gcttttgcaa cgttactttc atgtacccgt gaatgtaatt
     1141 gtgtaaaaaa agtaaatgga gaagttacct ataccgaagt ggtaaagcaa cgaggcagtt
     1201 gtaaagagtt aaatgaaaga gcagaaattt ttgatcttgt aactgaatat acatgtgaaa
     1261 aaaaataact ctaatatatt aatatattag agttatatat gttgtattta gttgagtttt
     1321 tttatccttt taatgcatta gcaccgctaa ctatttcaag aatttcatta gtaattgaag
     1381 cctgacgagc tttattgtaa tcaattcgta aagatttcaa aagttcacct gcattatctg
     1441 tagctttgtg catagcagtc atacgagcac catgttccga tgcaaaagaa tcaagcatag
     1501 cactatagaa ttgtatttta agtgaacgag gaatcaattc tgtgataata tattctttgg
     1561 taggttcaaa aatgtaatca tagttaaatt tttttacact ttgtagagct atagtctgag
     1621 gagcttcaat aggtaaaaat tgtttagtgg ttattatttg tgttgcagca tttttaaaac
     1681 tattatatac taaaagtact ttatcatatg tacccgaaac aaactcatgc ataatagttt
     1741 cagatagttt tgctacattt tcatatgata aattatcgta aatatcaaca aacgattttg
     1801 taggttcaaa tcctttggac ttaagcatgt ctcgtacttt tttaccaaca gcatatatat
     1861 atacctgttt tttagcatgc tgcaccgcaa attcattttg aataagcgaa atagtcgttt
     1921 ttccaatgtt tgaattaaag ccaccgcata atcccccatt agaggcaatt gcaactatta
     1981 gaatacgttc ttcgcgacga gctttcccgt atatattttc ctgattagca tcagaatcgg
     2041 cacttacatt ggttaatatt tcttgcaatt tttctgaata tggacgcatt tgctgaacag
     2101 cttgttgagc tttacgtaat tttgcagccg aaaccatttt catagcactg gtaatttttt
     2161 gtgtattatt taccgatgct attcgagtac gtatttcttt tatacctgcc atacaatttt
     2221 agaatttaag aatcattatt tgtatttagc tgacacttct ttagtagctg catcaagcac
     2281 atttgttatt tcatcgttaa aaacgccctc ggcaattttt tgtaatgtgt ctttgtgttt
     2341 taattccaag tattctatat agtctttttc gaattcacga actttatgta taggaacatg
     2401 ttgcaaagca ccttttgtac cacaataaat tattgctact tggtgttgta caggtaccgg
     2461 agaatactgt ccttgtttta aaatttcaac atttctgcgt cctttatcaa gtacagccat
     2521 tgttgcagca tcaaggtcag agccaaattt cgagaaagct tcaagttcgc ggaattgagc
     2581 ttggtcaac
//
LOCUS       DAOTOW010000288         2587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31161_length_2587_cov_2.690363, whole genome shotgun sequence.
ACCESSION   DAOTOW010000288 DAOTOW010000000
VERSION     DAOTOW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31161_length_2587_cov_2.690363"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="P5243_05940"
     CDS             complement(<1..351)
                     /locus_tag="P5243_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRS19024.1"
                     /translation="MGRAFEYRRAAKEKRWDKMSRIFPRLAKAITLAAKEGGPDPEMN
                     AKLRTAILNAKAQNMPKDNIDAAIKRASGKEAEAYTEVIYEGKGPHGVLVVVECATDN
                     TTRTVANIKSYFNKT"
     gene            complement(368..901)
                     /locus_tag="P5243_05945"
     CDS             complement(368..901)
                     /locus_tag="P5243_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19025.1"
                     /translation="MGAPIQKPRVIKDFEKLDQAIQEQIKLTYPTGFYEHLITYTDKD
                     GNERYALPFETDDKYYMVRMSISEAKRLISDDDDYDDNGILRNDVQEMYQEKYQDIDY
                     LGDFDAIVTPDIEIDDKEIDDEDDSDEDEKPTMKKKSKFDDDDDDDDDFDEDDLDNED
                     FDDEDFDDEDEEEDDEE"
     gene            complement(1079..2464)
                     /gene="glmM"
                     /locus_tag="P5243_05950"
     CDS             complement(1079..2464)
                     /gene="glmM"
                     /locus_tag="P5243_05950"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRS19026.1"
                     /translation="MTLIKSISGIRGTIGGFPGENLTPVDIIAFSSAFGQWIIEKQPQ
                     ANRTIVIGRDARISGSMVNGLISNTLVAMGFTVIDIGLATTPTVEIAVPAENAAGGII
                     ITASHNPKQWNALKLLNNKGEFVSASDGEHILKIAEKSAFAYNTIESIGNHIYNNTYT
                     QKHIDAIVALPLVNKKAITNAQFTIAIDCVNSVGGIVIPQLLEALGVKTVIPLYCEPN
                     GIFPHNPEPLPEHLTEIAQTVVANKADVGFVVDPDVDRLAIINEDGSMFGEEYTLVAI
                     ADYVLHHTPGNTVSNMSSTRALRDITEQKGYSYTASAVGEVNVVTTMKETHAVIGGEG
                     NGGVIYPPLHYGRDALVGIALFLSHLATCGKSCKELRNTYPNYSISKNKIELTPNINV
                     DTILEKIAQTYSREHVNTIDGVKIDFADSWVHLRKSNTEPIIRIYAEATTDIRANELA
                     NQIIKDIKSII"
BASE COUNT          763 a          456 c          385 g          983 t
ORIGIN      
        1 ggttttatta aaatatgatt taatattggc aactgtgcga gttgtattat cagtagcgca
       61 ttctactaca actaaaactc cgtgtgggcc tttgccttca tatattactt cggtatatgc
      121 ttcagcttct ttgccgctgg cacgttttat ggccgcgtca atgttatctt taggcatatt
      181 ttgagctttt gcatttaaaa ttgctgttcg caattttgca ttcatttcag gatcaggacc
      241 accttctttt gctgctaaag taatagcttt tgctaatctt ggaaaaattc gagacatttt
      301 atcccatcgt ttttcctttg ctgctcgcct atattcaaat gctcttccca ttatattctg
      361 tttttattta ttcttcatca tcttcctctt cgtcttcatc atcaaagtct tcatcatcaa
      421 agtcttcatt gtctaaatca tcttcatcaa aatcatcatc gtcatcgtca tcgtcatcaa
      481 actttgattt ctttttcata gttggttttt catcttcatc agaatcgtct tcgtcatcaa
      541 tttctttgtc gtctatttca atatcaggtg ttactatggc atcaaaatct cctaaataat
      601 caatatcttg atatttttct tgatacatct cctgaacatc atttcttaat attccattgt
      661 catcatagtc gtcatcatcg ctaattaaac gttttgcttc gcttattgac atacgtacca
      721 tataatattt atcatcagtt tcaaaaggta acgcatatcg ttcattacca tctttatcgg
      781 tataggtaat gagatgctca taaaatcctg taggatacgt aagtttaatt tgttcttgaa
      841 ttgcttggtc aagtttttca aaatctttga ttactcgagg tttttgaatt ggtgctccca
      901 tagtttactg tgtattgtta attttcttct tttagttttt ttaaattttt gccaaataaa
      961 taaaaaatac ttgttataat aaatagtttc aaaaaaaaaa tttgtacaaa ttaatatagt
     1021 ttacaaatac ctatactttt ttgaaagtaa tgtttgtgta aaatctatat atatatttct
     1081 atattataga tttaatatct tttataattt gattggcaag ttcgttagca cgtatatcgg
     1141 ttgttgcttc ggcatatatt ctaattatag gttctgtatt tgatttacgt aagtgaaccc
     1201 atgaatctgc aaaatcaatt ttaacaccgt caattgtgtt tacatgttcg cgtgaatatg
     1261 tttgagcaat tttttcaaga atagtgtcaa catttatatt tggcgtaagt tcaattttat
     1321 ttttactaat actatagtta ggatatgtat ttcgtaattc tttacatgat ttgccacatg
     1381 ttgctaaatg agaaagaaat agagctattc caaccaaagc gtcacggcca taatgaagag
     1441 ggggataaat aacaccaccg ttgccttcgc ctccaattac agcatgtgtt tctttcattg
     1501 ttgttactac atttacttca ccaactgcag aagcagtata tgaataccct ttttgttcag
     1561 taatatctct tagtgctcgt gtagacgaca tgttgcttac cgtattaccc ggagtatgat
     1621 gtaatacata atcggctatt gcaaccaaag tgtattcttc gccaaacata ctaccgtctt
     1681 catttattat tgcaagtcgg tctacatcgg gatcaacaac aaaccccaca tcagctttgt
     1741 ttgcaacaac cgtttgtgca atttctgtta aatgttccgg taatggttcc ggattatgag
     1801 gaaatattcc gttaggttca caatatagtg gaattaccgt tttaacacca agagcttcta
     1861 ataattgagg tattacaata cctccaacag aattaacaca gtcaatagca attgtaaatt
     1921 gagcattagt aatagctttt ttattaacta aaggtaatgc tacaatagca tctatatgtt
     1981 tttgtgtgta tgtattatta tatatatgat tacctatact ttcaattgta ttatatgcga
     2041 atgctgattt ttcggcaatc tttagtatat gttcgccgtc tgaagcgctt acaaattcac
     2101 ctttattatt cagtaatttc aatgcgttcc attgttttgg attgtggctt gccgtaatta
     2161 taataccacc agctgcgttt tctgcaggta cagcaatttc aactgttggg gtagtagcta
     2221 aaccaatatc tataacagta aatcccatgg caacaagtgt atttgaaata agaccgttta
     2281 ccatagaacc tgaaatgcga gcatctctgc ctattacaat tgtacgattt gcttgtggtt
     2341 gtttttctat aatccattga ccaaaagcag atgagaaagc aatgatatca accggtgtta
     2401 aattttcgcc cggaaaacca ccaatagttc ctcgtatacc tgaaattgat ttaattaacg
     2461 tcatacattg aaaagtatta aattttatac aaaaatcata aataaacaga tttgatgagc
     2521 aatacaatag aatagtttta cacattttat taaatgcaat gtttatttac atatttttat
     2581 agaatga
//
LOCUS       DAOTOW010000289         2579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31282_length_2579_cov_3.355388, whole genome shotgun sequence.
ACCESSION   DAOTOW010000289 DAOTOW010000000
VERSION     DAOTOW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31282_length_2579_cov_3.355388"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="P5243_05955"
     CDS             <1..801
                     /locus_tag="P5243_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19027.1"
                     /translation="RTATNGEEGLAVFKEYKPHIVLTDINMANMNGIELLAAIKKIQP
                     QTIVIMLTAFNSEDFVIEAMRYGANNYLKKPIFRDNIIGLLRKYKNIILSRNTHIKIQ
                     SFVKRHSYTMEISNDIELLPYVINYLIESVELVFKEDECMGIRLGLDEIVINAIEHGN
                     LNISYVEKTQAIKNNTLEKLHKSRRNAPENIHKTVTIHFEMNKEYCEWTIKDQGKGFN
                     PSLIPNPILTESAESLHGRGIFITQFQFDEMEYSENGTKVRLRKNKIR"
     gene            807..>2579
                     /locus_tag="P5243_05960"
     CDS             807..>2579
                     /locus_tag="P5243_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRS19028.1"
                     /translation="MRFFAGVVISFVLYFSIFAQNQLVIPIVNSHDISGNNQSAVVGK
                     QIPDSIVFKVVDSHNNPMPGIPVNVAITKEPHWGSKSVLGDTLLYTDSQGMCATTYTL
                     GNKKGLYQITASIFSKSPHNAITYSFEAKSSLWLFFLLAGVIGGLVFFLYGMNTMSSG
                     LQKTAGDKMRTILSSLTRNNFIGLGIGALVTAIIQSSSATSVMLVSFVHSGLMRFKQT
                     LSILLGATIGSTITVQLISFKLTDYSLAFVAVGGALYMFAKKYSLRFWGESMFGFGLL
                     FFGMVVMSESIAPIKSSPLFIDLFIHLQHPVLGIIVGALFTAIVQSSAASIGIFIILA
                     SQGLLTIESSIALVLGANLGTPVTALIASLKTSHDAKRVAITLLVYKILLVLFFIWFI
                     TPIAHYVSQSITNQNYSDELPRAIANIHTFFNITIAIIIFPCISLVEKLILRIKPLKS
                     YEIQKQKTRYIDTTFINQPAIAMQLAKEETMRLGRKIQLSLELILKPFIENNPDYLEK
                     LNLQRIEAKDLRDAIKEYLLQTAQFDASKQRSEELFAIIHTLTELSHINDALTKVLHR
                     RAEKWIERNYEFTESEKQEIEAYHT"
BASE COUNT          902 a          356 c          444 g          877 t
ORIGIN      
        1 agaacggcta caaatggaga agagggactt gccgtgttta aggaatataa accacatatt
       61 gttttgactg atataaacat ggcaaatatg aatggtattg aattacttgc tgctattaag
      121 aaaattcaac ctcaaactat tgtcattatg cttaccgcat tcaattcaga agattttgta
      181 attgaggcaa tgcgatatgg agccaataat tatttgaaaa aaccaatttt tagagataat
      241 attattggat tgcttagaaa atataaaaat ataattcttt ccagaaatac ccatattaaa
      301 attcagtctt ttgttaaacg gcattcttat actatggaaa taagtaatga tatagagttg
      361 ttaccatatg ttataaatta tttgattgag agtgttgaat tagtctttaa agaagatgaa
      421 tgcatgggaa ttcgattggg attagatgaa atagttataa atgccataga acacgggaat
      481 ttgaatattt cttatgtaga gaaaacacaa gcaattaaga ataatacatt agaaaagtta
      541 cacaaaagcc gacgaaatgc tcctgaaaat atacataaaa cagttactat tcattttgaa
      601 atgaataaag aatattgtga gtggacaata aaagatcaag gtaagggatt taatccgtcg
      661 ttaattccta atccaatatt aacagaatct gccgaatctt tacatggacg tggtattttt
      721 ataacacaat ttcaatttga cgaaatggaa tattctgaaa acggtacaaa agtaaggtta
      781 agaaaaaata aaataagata ggtggtatgc gtttttttgc aggggttgtt atttcttttg
      841 tattgtattt ttccatattt gctcaaaatc aacttgtaat tccaatagta aattcacacg
      901 atatttccgg caataatcag tcagctgttg ttggtaaaca aatacccgat tctattgttt
      961 ttaaggttgt tgattcgcat aataatccaa tgcccggtat tcctgtaaat gttgctataa
     1021 ctaaagaacc acattggggg tcaaaatctg tattgggcga taccctattg tatactgatt
     1081 ctcaaggaat gtgtgccaca acatatacac taggtaataa aaagggattg tatcaaatta
     1141 ctgcaagtat tttttcaaag tcaccacaca atgctataac atattcattt gaagcaaaaa
     1201 gtagtttatg gctatttttc ttgcttgccg gtgttatagg aggactagtt tttttcttgt
     1261 atggcatgaa tactatgagt tccgggttac aaaaaactgc cggtgacaaa atgcgaacaa
     1321 ttttaagtag tcttactagg aataatttta ttggtttagg aataggagct ttagttaccg
     1381 caataattca aagtagcagt gcaacatctg taatgcttgt aagttttgtg cattccggat
     1441 taatgcggtt taaacaaaca ttaagtattt tgttgggagc aaccataggt tcaactatta
     1501 ctgttcagtt aatttcattt aaacttaccg actattctct tgcattcgta gcagtagggg
     1561 gggcactgta tatgtttgct aaaaaatatt ctcttcgttt ttggggagaa tcaatgtttg
     1621 gttttggatt attgtttttt ggtatggtgg taatgtcaga atctattgct cctattaaaa
     1681 gttcaccatt atttatagat ttatttatac atctgcaaca tccggtatta ggtattattg
     1741 ttggagcttt gtttacagct atagtacaaa gcagtgctgc aagtataggt atttttataa
     1801 ttttagcctc acaaggatta ttaactatag agtctagtat agcgttggtt ttaggagcta
     1861 atttaggaac tcctgttacc gctttaatag catcgcttaa aacatctcat gatgctaaac
     1921 gagtagcaat tacattatta gtatataaaa ttcttttagt gttatttttt atttggttta
     1981 ttacacctat agctcattat gtttcgcagt ctattacaaa tcaaaattac agtgatgaat
     2041 taccaagagc aattgctaat attcatacgt tttttaatat cactattgca attataattt
     2101 ttccttgtat ttcattggtt gaaaaactaa tactacgtat aaaaccactc aaatcatatg
     2161 aaatacaaaa acaaaaaacc cgatatattg atacaacatt tattaatcaa cctgctattg
     2221 caatgcaatt agctaaagaa gaaacaatgc gattggggcg taagattcaa ctgagtttag
     2281 aattgatatt gaaacctttt atagaaaata atcctgatta tttagaaaaa cttaatttgc
     2341 aacgaataga agcaaaagat ttacgtgatg ctataaaaga atatttattg caaactgctc
     2401 agtttgatgc atcaaaacaa cgttccgaag aattgtttgc aataattcat actcttacag
     2461 aattatcgca cattaatgat gcgttgacta aagttttgca tagaagagca gaaaagtgga
     2521 ttgaaagaaa ttatgaattt actgaatctg aaaaacaaga gatagaagca tatcatact
//
LOCUS       DAOTOW010000290         2574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31351_length_2574_cov_3.252878, whole genome shotgun sequence.
ACCESSION   DAOTOW010000290 DAOTOW010000000
VERSION     DAOTOW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31351_length_2574_cov_3.252878"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..>2574)
                     /locus_tag="P5243_05965"
     CDS             complement(137..>2574)
                     /locus_tag="P5243_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS19029.1"
                     /translation="NSQEGVVFKAEQIEYVFKGGNVGLHKLDLQEESGNLVGIMGASG
                     AGKSTLLNVLNGYYKPTHGRVLINGVDLHNDKEGVLDGLIGFVPQDDLLIKELTVYQN
                     LYYNAKLCFDNLTEEEIREKVDRVLQSLGLFERKNNIVGDELRKEISGGQRKRLNIAL
                     ELIREPAILFLDEPTSGLSSKDSENIMDLLKELALKGKLVFVVIHQPSSDIFKTFDKL
                     LILDTGGYQIYYGAPVDSIMYFKERMHQADWYVSECPTCGNVNPEQIFNITEAKILNE
                     DGTPMLNSKRQEIRKYAPTDWYEMYKKTSANNDAIKEKPIPKGLPEITFKIPNWIRQF
                     KVFVVRDVLSKISNMQYVMINSLESPIIAVFLAFIIKYWDVDEGNQIGYTLMNNDNIP
                     VYLFMLVICAYFIGLTVSAEEIFRDQKIRRRESFLQLSWSSYLMSKIAILLVISAIQS
                     FVFILLGNAILEIHGMFFQYWFIAFSVWSFANIVGLIISDSFNDVKTIYILIPFMVIP
                     QIILSGIIVSFDKLNPRISNPQDIPFYGEVITSRWAYEALAVYQYSQNDYEKNFFKLD
                     MQISECSYKKGEWSNQLTNRINDSERILDDKTKLDVLQKNLTVIYNELYKEGTKNRAY
                     RFDNIDKLNIHDITPTVLADAREHVSDIKQYYIQKNNYVRKAKDSLTYAMIETMGKEA
                     VRKMNVPESQITPKLIDSVGNAVFLQLKKTHYNESLEEFCKNENDLTVLIEYKGHLVQ
                     KNDPVFMLPKGNFIKAHFYAPVKPFFGYYVSTYWANAIIIWLMSIVSYVFLYYRLLRK
                     TIDFLGNIKLRKK"
BASE COUNT          845 a          441 c          376 g          912 t
ORIGIN      
        1 ggggtatcta aacaaaacct ataactaata gataccggat tgcaatatgt atttgtatga
       61 gtcggtatat tagaatgagt acattgtata aaacacaatg taagaataca catacaccca
      121 aacagcaatt tcattgttat ttttttcgta atttaatatt acctaaaaag tctatggttt
      181 tgcgtaataa acggtaatat aagaacacat atgaaacaat agacataagc caaataataa
      241 tagcatttgc ccaatatgta ctaacgtaat atccaaaaaa tggtttaaca ggtgcataaa
      301 aatgagcttt tataaaatta ccttttggca acataaatac cgggtcattt ttttgtacca
      361 aatgaccttt atattctatt aatacagtta aatcgttttc gtttttacaa aattcttcaa
      421 gactttcgtt gtaatgcgtt tttttgagtt gtaaaaacac ggcatttcca acactatcaa
      481 taagttttgg tgtaatctgt gattcgggca cattcatttt tcgtactgct tctttaccca
      541 tagtttcaat catagcatag gtaagagagt ctttagcttt tctaacgtaa ttgttttttt
      601 gaatgtaata ttgttttatg tccgaaacat gttcgcgagc atcagccaat accgttggag
      661 taatatcatg tatattcaat ttgtctatat tatcaaatct atatgctctg tttttagtac
      721 cttctttgta cagttcatta tatattactg taaggttttt ttgcaataca tcaagttttg
      781 ttttatcatc aagtattcgt tcagagtcgt ttattctgtt ggtaagttga tttgaccatt
      841 ctcctttttt ataactgcat tcgcttattt gcatatctaa tttgaagaaa tttttctcat
      901 aatcattttg agaatattga tatacagcaa gagcttcgta agcccatcga gaagttatga
      961 cttcgccata aaaaggaata tcttgcgggt ttgaaatgcg aggatttaat ttgtcaaaac
     1021 ttacaatgat accacttaat ataatttgag gaattaccat aaacggaata agtatgtata
     1081 tggtttttac atcattaaat gaatccgaaa ttataagacc tacaatattt gcaaaactcc
     1141 acacgctaaa tgcaataaac caatattgga aaaacattcc gtgaatttct aaaatagcat
     1201 tgcctaaaag aataaacaca aatgattgta tagcagaaat aactaacaaa attgcaattt
     1261 ttgacattaa ataactagac caacttaatt gcaaaaatga ttcacgacga cgaatttttt
     1321 gatctctgaa aatttcttcg gcacttaccg taagaccaat aaaataagca catattacta
     1381 acataaataa atacacgggt atattgtcat tattcatgag agtatagcct atttgatttc
     1441 cttcatctac atcccaatat tttatgataa aagctaaaaa cacagcaata attggtgatt
     1501 ctaaagaatt aatcataaca tattgcatgt tagatatttt agaaagtaca tcgcgaacta
     1561 caaatacttt gaactgacgt atccaattgg gaattttaaa agtaatttcc ggaagacctt
     1621 ttggtattgg tttttctttg atagcatcat tatttgccga tgttttttta tacatttcat
     1681 accaatcggt aggagcgtat ttacgtattt cttgccgttt gctgttgagc attggcgtac
     1741 cgtcttcatt gagaattttt gcctcggtta tattaaaaat ttgttcagga tttacattac
     1801 cacaagtagg acattcactt acataccagt cggcctgatg cattcgttct ttaaaataca
     1861 taatagaatc tacaggagct ccataatata tttgatatcc tccggtatcg agtatcaata
     1921 atttgtcgaa tgttttaaat atatccgatg atggctgatg aattactaca aataccaatt
     1981 tccctttaag agcaagctct ttcaaaaggt ccataatatt ttcggagtct tttgatgaaa
     2041 gacccgaagt tggttcatct aaaaataaaa ttgccggttc gcgaataagt tccagtgcta
     2101 tgtttaatcg tttacgttgg ccaccgctaa tttctttacg taattcatca cctacaatat
     2161 tatttttacg ttcaaataaa ccaagactct gtaatacgcg gtcaactttt tctctaattt
     2221 cttcttctgt taaattatca aaacacaact ttgcattgta atataagttt tgatatacgg
     2281 ttaattcttt tattaacaag tcgtcttggg gaacaaaacc aattaaaccg tccaatacgc
     2341 cttctttatc gttatgcaaa tcaacaccgt ttatgagaac tctaccgtgt gtaggtttgt
     2401 agtacccgtt caaaacattg agcaatgtag atttaccggc acctgaagct cccataattc
     2461 ccaccagatt gccggattct tcttgtaaat caagtttgtg taaccctaca tttccacctt
     2521 taaatacata ttctatttgt tcggctttga atacaacacc ttcctgagaa ttat
//
LOCUS       DAOTOW010000291         2568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31440_length_2568_cov_2.534023, whole genome shotgun sequence.
ACCESSION   DAOTOW010000291 DAOTOW010000000
VERSION     DAOTOW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31440_length_2568_cov_2.534023"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="P5243_05970"
     CDS             complement(<1..1667)
                     /locus_tag="P5243_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRS19030.1"
                     /translation="MTISQLFDVHEWHIIESTFDANRHKIAESIFSIGNGHMGQRANF
                     EETYSGKTLSGNYIGGVFYPDKTRVGWWKNGYPDYFAKVLNAPNWIGIDIYINREKCD
                     LAKVAFISFKRVLNMKEGTLHKTCEVELANKIRLLLSSTRFVSMHMVEVGAIEYCITP
                     LNADCSIQIESYINSDVHNEDANYNDQFWESVSQNYSNNGAVAVDRTKKTQYDVPQFT
                     VATGMHNQFFIQNKKQSGISTFSKKGLRIIEKAELKLQQQQTCKIIKIATQVSSLNHE
                     EKTIEQAAYTVLEKAVETGYKQLLKNHIAIWADKWKLCDITIKGDTKAQQGIRFNIFQ
                     LQQTYTGNDSRLNIGPKGFTGEKYGGSTYWDTEAFCLSFYLATSPETVAKNLLLYRYK
                     QLDRAIKNAEKLGFSNGAALYPMVTMTGDECHNEWEITFEEIHRNGAIAYAIYNYVNY
                     TGDKSYLVEYGLEVLIAIARFWTQRVTLSPTKRKYMILGVTGPNEYENNVNNNWYTNY
                     FARWCLQYTLETISWVKRVANQEFTELSHRIAFDENYEVIRWTDIIK"
     gene            complement(1822..1905)
                     /locus_tag="P5243_05975"
     tRNA            complement(1822..1905)
                     /locus_tag="P5243_05975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1869..1871),aa:Leu,seq:gag)
     gene            complement(1975..>2568)
                     /locus_tag="P5243_05980"
     CDS             complement(1975..>2568)
                     /locus_tag="P5243_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRS19031.1"
                     /translation="LTQHGYIVVAVDCRGTDGRGSNFRKQTYLNLGEKESYDFAATAQ
                     YLRTLPYVDKQNITIQGWSYGAYMALLTAAKYPDSYNSVIAIAPVTKWELYDNIYTER
                     YMQTPTLNPEGYKNSSVFTYIQNIKAPILLAHGTADDNVHIQNTYELISELIKNEKDF
                     ETLIFPNDEHSLFGKNSRIYLYSKYIDFLDNHSKSNK"
BASE COUNT          726 a          436 c          424 g          982 t
ORIGIN      
        1 ttttttataa tatctgtcca gcgtataacc tcataatttt catcaaatgc tattcgatga
       61 gataattcgg taaattcttg attagcaact cgttttaccc aacttatagt ttcaagagta
      121 tactgcaaac accatcgggc aaaatagttt gtgtaccaat tattgtttac gttgttttcg
      181 tattcattgg ggccggtaac tcctaaaatc atatattttc gtttggtcgg agatagtgtt
      241 acacgctgag tccaaaatcg agctattgca attaacactt cgagtccgta ttctaccaaa
      301 tatgatttgt cgcctgtgta atttacatag ttgtatatag catatgcaat agcaccgttt
      361 ctatggattt cttcaaaggt tatttcccat tcgttatgac actcatctcc ggtcatggta
      421 accataggat ataatgctgc tccgtttgaa aatccaagtt tttcggcatt ttttatggca
      481 cgatcaagtt gtttatatct atagagtaat aaattttttg caacagtttc gggtgatgta
      541 gctaaataaa atgatagaca aaaagcttcg gtatcccaat atgtgctgcc tccgtatttt
      601 tcgcctgtaa agcctttggg gccgatattt aatctactgt cattacctgt ataggtttgt
      661 tgtaattgaa aaatgttgaa tcgaataccc tgttgagctt tggtatcgcc ttttatggtg
      721 atgtcacaca atttccattt gtcggcccat atggcaatat ggttttttaa taattgtttg
      781 taccctgttt caactgcttt ttctaaaact gtatatgcag cctgttctat tgttttttct
      841 tcatgattga gtgacgaaac ttgagtggca atctttataa ttttacaggt ttgttgttgt
      901 tgtaatttca attctgcttt ttctattatt cgtaatcctt tttttgaaaa ggtagaaata
      961 cccgattgtt ttttattttg tataaaaaat tgattgtgca tacctgtagc aacggtaaat
     1021 tgcggtacat cgtattgcgt tttttttgtt cggtcaactg ctacagctcc attgttgcta
     1081 tagttttgtg aaaccgattc ccagaattgg tcgttataat tagcgtcttc attatgaaca
     1141 tcagaattta tatatgattc tatttgaatt gaacaatctg cgttgagtgg tgttatacaa
     1201 tattcaatag ctccaacttc taccatatgc atacttacaa aacgtgtact cgacagtaat
     1261 aaccgaattt tgtttgctaa ttctacttca catgtcttgt gtaatgttcc ttctttcatg
     1321 ttcaaaacac gtttaaatga aataaaggca acttttgcta aatcacattt ttctctgttt
     1381 atgtaaatat ctattccaat ccaattggga gcattaagaa cttttgcaaa ataatcggga
     1441 tatccatttt tccaccaacc aactcgtgtt ttgtcaggat aaaaaacacc tccaatataa
     1501 ttgcctgata aagtttttcc ggaatatgtt tcttcaaaat tagcacgttg ccccatgtgt
     1561 ccgtttccga tactaaaaat actttcggct attttatggc ggttagcatc aaatgttgat
     1621 tcaataatgt gccattcgtg tacatcaaac agttgtgaaa tagtcatatt atacgtattt
     1681 atattattgt tacaataata gtaatttttt gtgttatact gttattcaaa ctatatatat
     1741 gttatgaaat acaagaattt ttggcgtaca gtggtcttta taaaacaaaa cgcttctttt
     1801 tataagaagc gttttgtttt gtgcccggaa caggatttga acctgcacac ccttaagagc
     1861 acatggccct caaccatgct tgtctaccaa ttccaacatc cgggctcaat tgatatgcaa
     1921 aaatacattt tttttaaagt aacacaacct tagcatttaa tttttcagtg ttttttattt
     1981 attagatttt gaatggttgt ctaaaaaatc tatgtatttt gagtatagat atattcggga
     2041 gtttttccca aataaactat gttcgtcgtt ggggaagatg agtgtttcaa aatctttttc
     2101 attcttaatt aattctgaaa tgagttcata tgtgttttgt atatgtacat tatcatcggc
     2161 tgttccatga gctaatagaa ttggagcttt tatattttgt atataggtaa atacactact
     2221 gtttttgtac ccttcaggat tcaatgttgg ggtttgcatg tagcgttctg tataaatatt
     2281 atcatataat tcccattttg taacaggggc tatggcaatt accgaattat atgaatcagg
     2341 atattttgct gctgttagta atgccatata ggctccataa ctccaacctt gaatagtaat
     2401 attttgttta tcaacataag gtaatgttcg taaatattga gctgttgcag caaaatcgta
     2461 actttctttt tcgcctaagt tcaaatatgt ttgtttcctg aaattactgc ctctaccatc
     2521 ggtacctctg caatctacag ctactacaat atatccatgt tgggttag
//
LOCUS       DAOTOW010000292         2567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31459_length_2567_cov_2.415207, whole genome shotgun sequence.
ACCESSION   DAOTOW010000292 DAOTOW010000000
VERSION     DAOTOW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31459_length_2567_cov_2.415207"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1334)
                     /locus_tag="P5243_05985"
     CDS             complement(168..1334)
                     /locus_tag="P5243_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS19032.1"
                     /translation="MKKVIVLGGGFAGVEAAIRLQKSGFCDVTLVSDRDFLYLYPISI
                     WIPTHGISPDSVKVSLYEIQKKHGFSLIIDSVQNISPKENTILCSKQILTYDYVIIAL
                     GANKLSHNGIEHTYSICGKPEISIEIQKTLDAIIQKKQGTIAIGFGGNPKDMSAVRGG
                     PAFEFAFNVHTYLQSKNLRNSIEIHMFAPMKEPGARMGKKALSMVDTMLNKANIHKHF
                     GKKISQFEASKIIFEDNSELQADLIMYIPASQGHTALQNSNLPLNNAGFVIIDDTCKV
                     EGTSNVYAIGDSAAIEGYDWIAKQGHLAELMGRNAAYNIIQSIQNSTKRKGYKEHLNI
                     LCVMDTGNGAAFVFRNHKRAFVIPMPIVGHWLKKSWGVYSKLTKLGKIPHLPGM"
     gene            complement(1416..2129)
                     /locus_tag="P5243_05990"
     CDS             complement(1416..2129)
                     /locus_tag="P5243_05990"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRS19033.1"
                     /translation="MKQVFVLLLLATTSVFAQNYTNVSASEFKSLIEKKNGVIADIRT
                     PAEFERGYIPGATLYNSQDPNIGTLLLSIPKTKPVYVYCYSGARSRQIATFLANNGYT
                     QVYNLQRGLIDWNSMGYTLQTPANAAANSAQPHAYTLQKHTQLINKQSLVFIDYYAPW
                     CAPCKQMMPMIDELTAQYKGKILIEKVNTDASKEVMQQLQIKGVPYLVLYKDGKPVYT
                     QYGMVSKEVLQTEFAKYIK"
     gene            complement(2140..>2567)
                     /locus_tag="P5243_05995"
     CDS             complement(2140..>2567)
                     /locus_tag="P5243_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS19034.1"
                     /translation="SVIALSYIAAQRTFYGYNEMAEAKAMLESIARSFGYIYGRDKKI
                     RVNTISQSPTLTTAGQGIKGLEDLLDFSERMSPLGNATAEDCADYCIVMFSDLTKKVT
                     MQNLFNDGGFSNIGMSWRAMNVYSTSLEDMKNNIDKIID"
BASE COUNT          763 a          412 c          418 g          974 t
ORIGIN      
        1 taatttttgc aagaaaaatt tatctataca cattatgtat acatgcaaat agttagtatt
       61 gaattcttta aagttattgt tgaatgatta ggtatgtaaa ttactcatac acatgccttg
      121 tgggtatgag taatttacta ttaattttta cattataaga atgtttacta cataccgggt
      181 aagtgtggaa tttttcctaa tttggttagt ttactgtaaa ctccccatga tttttttagc
      241 caatgtccaa ctataggcat aggtattaca aatgctcgtt tgtgatttct aaatacaaat
      301 gcggcaccat taccggtatc cattacgcat aatatattta agtgttcttt gtatcctttt
      361 cgttttgtag agttttgaat agattgtatt atattatatg ctgcatttct gcccataagt
      421 tcagctaaat gaccttgttt tgcaatccaa tcatatcctt ctatagcagc tgaatctcct
      481 atagcatata catttgaggt tccttcaact ttacatgtgt catctattat aacaaatcca
      541 gcgttattaa ggggtaagtt ggaattttga agtgcagtat gtccttgtga agcaggtata
      601 tacataatta aatcggcttg taattctgaa ttatcttcaa atattatttt tgatgcttca
      661 aattggctta tttttttacc gaaatgtttg tggatgtttg ctttatttag catagtatct
      721 accatactca atgctttttt acccatacga gctccaggtt ctttcatggg ggcaaacatg
      781 tgaatttcta ttgagtttct aaggtttttt gattgtaaat acgtatgaac attaaatgca
      841 aattcaaatg caggtccgcc tcttactgca gacatatctt tgggattgcc tccaaaccca
      901 atagctatag tgccttgttt tttttgtatg atagcatcta atgttttttg tatttcaata
      961 gaaatttccg gtttgccaca aattgagtat gtatgttcta ttccattgtg gctcaatttg
     1021 tttgctccta atgctattat tacatagtca tatgtaagta tctgtttgct gcatagtatt
     1081 gtattttctt ttggcgaaat attttgaaca gaatctatta taagtgaaaa accatgtttt
     1141 ttttgtattt catacaatga aaccttaaca gaatctggtg atataccatg tgtgggtatc
     1201 caaatagaaa taggatataa gtataaaaaa tctctgtctg atactaaagt tacatcacaa
     1261 aaaccggatt tttgtaaacg tatagctgct tctacgcctg caaatccgcc tcctaataca
     1321 attacttttt tcatatgtta taaattacgt taaaatgaat attagtcgta ttttacatag
     1381 tttgcttaca ttgtatgagc aaaacgtatc aagaattatt tgatatattt tgcaaattcg
     1441 gtttgaagaa cttcttttga taccataccg tattgtgtat aaacaggttt tccgtctttg
     1501 taaagcacca aatatggtac accttttatt tgtaattgtt gcattacttc tttactggca
     1561 tcggtattta ccttttcaat taagatttta cctttatatt gagcagtaag ttcatctatc
     1621 attggcatca tctgtttaca tggggcacac catggcgcat aataatctat aaataccaat
     1681 gattgtttat taattaattg tgtgtgtttt tgaagagtat atgcatgagg ttgagctgaa
     1741 tttgctgcag cattagccgg agtttgaagg gtatatccca tagaattcca gtctataagt
     1801 ccgcgttgta aattataaac ttgtgtgtat ccgttgtttg ctaaaaatgt agcaatttgt
     1861 ctgctgcgtg ctcccgagta acagtaaaca tatacaggtt ttgttttagg aatagaaagc
     1921 aagagagtac caatattagg gtcttgcgaa ttgtataatg ttgctccggg aatatatcct
     1981 ctttcaaatt cagcaggagt tctaatatct gcaattacac cattcttttt ttcaattaaa
     2041 gatttaaatt cagaagcact tacattagtg taattttgtg caaaaacact tgttgttgcc
     2101 aataatagta atacaaatac ttgtttcatg taaatagaat tagtctataa ttttatcaat
     2161 attatttttc atatcttcta aactggttga atacacattc atagctcgcc aactcatacc
     2221 aatattagaa aatccaccat cgttaaataa gttttgcata gttacttttt ttgtgaggtc
     2281 agaaaacata acaatacaat aatcagcaca atcttcggca gttgcatttc ctagtggcga
     2341 catacgttcc gaaaagtcta ataaatcttc taatccttta ataccttggc cggcagtggt
     2401 tagggtaggt gattgtgata tggtgtttac tcgtattttt ttgtcgcggc catatatata
     2461 cccaaaacta cgtgcaatag attctaacat tgcttttgct tctgccattt cattgtagcc
     2521 atagaatgta cgttgtgctg caatataact taaagctatt accgatc
//
LOCUS       DAOTOW010000293         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31494_length_2565_cov_3.107171, whole genome shotgun sequence.
ACCESSION   DAOTOW010000293 DAOTOW010000000
VERSION     DAOTOW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31494_length_2565_cov_3.107171"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="P5243_06000"
     CDS             complement(<1..1105)
                     /locus_tag="P5243_06000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS19035.1"
                     /translation="MTLKNIIASILLLYSIIAYSQSTQQSFVLPVDIPVSLSANFGEL
                     RPNHFHGGLDFKTLSVVGKNMISIDTGYVSRIQVRPSGYGLALYITHPNGYTSVYGHL
                     LSFNAKIDAYVKQYQIANQKNIVDIQLQPHELPVARGEVIAFSGNSGSSQGPHLHFEI
                     RDSKTQNTLNPFLFYPQIIDKSRPKITAIGVYAMDSMSFIENSRDKKFYTVQSTPTSS
                     MHSIANPIQVWGNIGFSIQGNDFMQNSGHVYGYYSVKLLCNDTLLYARQIDEISFETT
                     ADINSFIDYTEYNKSKRYFEKAFVETNNSLQIYTHLKNKGIYTAKTIDTVTCEFIVGD
                     FHSNVAAVSFQLIQKPIADSILQKLKPYPKKYPC"
     gene            1248..1856
                     /locus_tag="P5243_06005"
     CDS             1248..1856
                     /locus_tag="P5243_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRS19036.1"
                     /translation="MNDTITFPIVEQFYSIQGEGYHTGKPAYFVRFGGCNVCCGWCDV
                     KESWNPYIHATESIHDIVQKIQVYNVDSVVVTGGEPLMHNLDEFCNILHSKSIQLFLE
                     TSGTHPISGTWDWICLSPKKHHKPLDSIYLYANELKVVIDESLDFEWAELCAHKVGAN
                     TLLYMQPEWNSYEKAIPGIVEYIKKHPQWRISIQTHKFMNIP"
     gene            1932..2339
                     /locus_tag="P5243_06010"
     CDS             1932..2339
                     /locus_tag="P5243_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19037.1"
                     /translation="MSKLKILISIICAMSVFQAYTQTYSTQNKRAIAAYEEAIKQFEL
                     RYFDQAELQLQTALKIDNTFSEAYYLLAGVYMQQGLTDKMIATLQTCVNVCADKTPWA
                     LYKLAFEQFDNGIYAEAKKNIDALAQKNYNLMK"
BASE COUNT          906 a          337 c          430 g          892 t
ORIGIN      
        1 tgcatggata tttcttgggg taaggtttca gtttttgtaa tatactatcg gcaatgggct
       61 tttgtattaa ttgaaatgaa acagcagcta cgttagaatg aaaatcacca acaataaatt
      121 cacatgttac agtatcaata gtttttgctg tatatatacc tttatttttt aaatgtgtat
      181 atatttgaag tgaattgtta gtttctacaa aagctttttc aaaatatcgt ttgcttttat
      241 tatattctgt ataatcaata aaactattta tgtcagctgt ggtttcaaat gaaatttcat
      301 caatttgtct agcatataat aaagtatcat tacaaagtaa ttttaccgaa taatatccat
      361 atacatgacc agaattttgc ataaagtcat taccttggat tgaaaaacca atatttcccc
      421 atacttgtat tggatttgca atactatgca tactgctggt tggagttgat tgaacggtat
      481 aaaatttttt atctcgtgag ttttcaataa aactcattga atccatagca tatacaccaa
      541 ttgctgtaat ttttggacga gatttatcaa ttatttgagg ataaaacaaa aatggattga
      601 gagtgttttg tgttttacta tcgcgaattt caaaatgcag atgagggcct tgtgaactac
      661 cgctgttacc tgaaaatgca ataacttcac ctcgagcaac aggtaattca tgaggttgta
      721 attgtatatc aactatattt ttttgatttg caatttggta ttgttttaca tatgcatcta
      781 ttttagcatt aaatgataat aagtgcccat atacacttgt atatccatta ggatgagtaa
      841 tatataatgc taatccatat ccggaagggc gtacttgtat acgagaaacg tatccggtat
      901 ctattgaaat catattttta ccaacaaccg aaagagtttt aaagtctaat cctccatgaa
      961 aatgattggg acgtaattcg ccaaaatttg ctgataatga taccggaata tctacgggta
     1021 aaacaaatga ttgttgtgtt gattgagaat atgcaataat agagtatagt agtagaattg
     1081 atgcaattat gttttttaat gtcatattat taaaattata agcttgtaac gtttatttgt
     1141 caatacctat tatgtttgac gataattaat aaaatagttt acttttgcaa atgtagaata
     1201 ttgcataatg gtaataacta taaattaatt gattttatac ataaattatg aacgatacta
     1261 tcacctttcc gattgtagag caattttaca gtatacaagg agaaggttat cataccggta
     1321 agccagctta ttttgtgagg tttggaggtt gtaatgtttg ttgtgggtgg tgtgatgtta
     1381 aagaatcatg gaatccgtat attcatgcaa ctgaatcaat acatgatata gtgcaaaaaa
     1441 tacaagtata taatgtagat tctgttgtag ttaccggggg tgaacctctt atgcacaatt
     1501 tagatgaatt ttgtaatatt ttgcactcta aaagcataca gttgttttta gaaactagtg
     1561 gtacacatcc tatttcagga acatgggatt ggatttgtct ttcgcccaaa aaacatcata
     1621 aacctttaga ttctatatat ttgtatgcga atgaattaaa ggttgtaata gatgaatctc
     1681 ttgattttga atgggcagaa ctttgtgcac ataaagtagg tgcgaatact ttattgtata
     1741 tgcagcccga gtggaattcg tacgaaaaag ctattccggg tattgtggaa tatataaaaa
     1801 aacatcctca atggagaata tctatacaaa cacataagtt tatgaatatt ccataaggta
     1861 tttatatttt tttggtaaaa tatttgaaat tggctactgg taattgtata ttgtagatta
     1921 aaataaaaat tatgagtaaa ttaaaaattc taataagtat aatatgtgca atgtcagttt
     1981 ttcaagctta cacacaaaca tattcaactc aaaataaacg ggcaattgct gcatatgaag
     2041 aagcaattaa acaatttgaa ttacgctatt ttgatcaagc agaattgcaa ttacaaaccg
     2101 cattgaaaat agataataca tttagcgaag catattattt gttggcaggt gtttatatgc
     2161 aacaaggatt aacagataaa atgatagcta cattgcaaac atgtgtgaat gtatgtgccg
     2221 ataaaacacc ttgggcttta tataaattag cttttgaaca atttgataat ggaatatatg
     2281 ccgaagcaaa aaaaaatatt gatgcattgg ctcaaaaaaa ttacaacctg atgaaataga
     2341 aaaagtaaat gatttacaaa aacgctgcga tatagcttta gaattattgt caaaaccggt
     2401 taatctaaaa cccaaaaata tgggtagtgc cattaattct caatatgatg attttcatcc
     2461 tgcaattact gttgacgaag aactatttat acttacatca aatattcctt tcagtgcaaa
     2521 atcatttcaa gaagatatgt tttattctgt aaaagaacaa ggtgt
//
LOCUS       DAOTOW010000294         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31543_length_2563_cov_2.207337, whole genome shotgun sequence.
ACCESSION   DAOTOW010000294 DAOTOW010000000
VERSION     DAOTOW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31543_length_2563_cov_2.207337"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="P5243_06015"
     CDS             <1..228
                     /locus_tag="P5243_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRS19038.1"
                     /translation="FGRGKQRGLKVGNVTYDELPKELLLIGIKERDKDFVINTIIEAA
                     RTGENGQFGDGKIFVTPVEETYTISNGMKEL"
     gene            258..638
                     /locus_tag="P5243_06020"
     CDS             258..638
                     /locus_tag="P5243_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRS19039.1"
                     /translation="MKLILAIIRIAKMNETKEALAEVGLPSFTATGVLGRGRGRGIGP
                     TFQETLSDPDKKSLLSAPGDMPRLKSKRMITLVVTDEKKDLAVETIIKVNKTGNSGDG
                     KIFVINAIDSVTVRTGVSGDASLD"
     gene            653..2269
                     /gene="nifD"
                     /locus_tag="P5243_06025"
     CDS             653..2269
                     /gene="nifD"
                     /locus_tag="P5243_06025"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HRS19040.1"
                     /translation="MKTKVTMDPERWKAEVLTKYPSKVAKKRAKSIVVNEGDEVKQIQ
                     ANIRTIPGIITQRGCTYAGCKGVVLGPTRDIINLVHGPIGCSFYAWLTRRNQTLPPTP
                     DSPNFMMYAFSTDMQDSNIVFGGEIKLKQAIREAFEIFKPKNIGIFSTCPVGLIGDDV
                     HAVAREMKAELGINIFGFSCEGYKGVSQSAGHHIANNGVFKHVVGLNDTPVEGKFKVN
                     LLGEYNIGGDAFEIERIFEKIGITLISTFSGNSTIDSFEQSHHADLNMIMCHRSINYL
                     AEMMETKFGIPWIKVNFIGADATAKSLRKIAQYFGDAGLATKVEEVIAEEMAFVAKEL
                     EPIKARTQDKTAMLFVGGSRAHHYQELFNELGMKTLSAGYEFGHRDDYEGRRVIPTIQ
                     IDADSRNIEEIHVEADPTRYKPRKTEAELKALEAQGLQFSEYEGMMTEMGKGALVIDD
                     ISHYEMEKLVEIYQPDVFCAGIKEKYCVQKMGIPLKQLHSYDYGGPYAGFEGAVNFYK
                     DIDMIVSTSIWKMVKAPWQKESVIEAEYAL"
     gene            2306..>2563
                     /locus_tag="P5243_06030"
     CDS             2306..>2563
                     /locus_tag="P5243_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRS19041.1"
                     /translation="MLLRHTTDKIIEREALTVNPAKTCQPVGAMYAALGIHGCLPHSH
                     GSQGCCSYHRSVLTRHYKEPVMAATSSFTEGSSVFGGQANLL"
BASE COUNT          893 a          456 c          547 g          667 t
ORIGIN      
        1 ttcggtagag gtaaacaacg tggtttaaaa gtaggaaatg ttacctacga cgagttacca
       61 aaagagttat tacttatagg tattaaagaa cgcgacaaag attttgtaat aaacacaata
      121 attgaagcag ctcgtaccgg cgaaaatgga cagtttggtg atggtaaaat attcgtaaca
      181 ccggttgaag aaacctatac catttcaaat ggcatgaaag aattataaca gttataaatc
      241 caaaaaaata aatcgatatg aaattaatac ttgctataat tcgcattgca aaaatgaatg
      301 aaactaaaga ggctcttgcc gaagtaggtc ttccttcgtt tacggcaacc ggagtgttag
      361 gcagaggacg cggacgagga attggtccta cgtttcaaga aacactcagc gatcccgaca
      421 aaaaaagttt actcagtgca cctggcgata tgcctcgttt gaaatcaaaa cgaatgataa
      481 cactggtagt aaccgacgag aaaaaagatt tagctgtaga aactattata aaagtgaata
      541 aaactggtaa cagtggcgac ggaaaaatct ttgtaataaa cgctattgac tctgttacag
      601 ttcgtaccgg agtaagcggc gatgcatcat tagattaatt aaggaggtaa agatgaaaac
      661 aaaagtaaca atggacccgg aacgatggaa agccgaagta cttacgaagt acccgtccaa
      721 agtagcaaaa aaacgtgcaa agtctatcgt ggtaaacgaa ggcgatgaag taaaacaaat
      781 tcaagctaac atacgaacta ttccgggcat aataacccaa cgcggatgta cgtatgctgg
      841 ttgtaaaggt gtggttcttg gacctactcg cgatatcata aacttagtgc atggtcctat
      901 tggctgtagt ttctatgcat ggcttacgcg tcgtaatcaa acattaccgc ctacacccga
      961 ttctccaaac tttatgatgt atgcctttag taccgacatg caagattcaa acatagtatt
     1021 tggtggcgaa atcaaattaa aacaagctat tcgcgaagca tttgagatat ttaaaccaaa
     1081 aaatatcggg attttctcta catgccctgt aggtcttatt ggcgacgatg tgcatgcagt
     1141 ggctcgcgaa atgaaagcag aacttggtat caatatcttc ggatttagtt gcgaaggata
     1201 caaaggtgtg agtcagtctg ctggtcacca tattgcaaac aatggtgtat ttaaacatgt
     1261 tgtgggatta aacgatactc ctgtagaagg taaattcaaa gtaaacttac ttggcgaata
     1321 caacattggt ggcgatgctt ttgaaattga acgaattttc gaaaaaattg gtattaccct
     1381 catctctact tttagcggta actctactat agattcgttt gaacaatcgc accatgccga
     1441 tttaaacatg attatgtgtc accgttctat aaactatctt gccgaaatga tggaaacaaa
     1501 attcggtatt ccttggatta aagtaaactt tataggtgcc gatgctaccg caaaatcatt
     1561 acgcaaaata gctcaatatt ttggggacgc aggtcttgct acaaaagtag aagaagtaat
     1621 tgccgaagaa atggcatttg tagcaaaaga attggaacca attaaagctc gcacacaaga
     1681 caaaactgca atgttgttcg taggaggttc acgagctcat cattatcaag aattgttcaa
     1741 cgaattaggt atgaaaactc tttctgcagg ctacgaattc ggacaccgcg atgattatga
     1801 aggtcgtaga gtaataccaa ctattcaaat agatgctgac agtcgaaaca ttgaagaaat
     1861 tcatgtagaa gccgatccta cacgctacaa accacgaaaa acagaagcag aactaaaagc
     1921 tcttgaggct caaggtttgc aattttctga atacgaaggt atgatgacag aaatgggaaa
     1981 aggagcattg gtaattgatg atataagcca ctacgaaatg gaaaaattag tagaaatata
     2041 tcaacccgat gtgttctgtg caggtatcaa agaaaaatac tgtgtgcaaa aaatgggtat
     2101 tccactcaaa cagcttcaca gctacgatta cggaggtccg tatgcaggat ttgaaggtgc
     2161 cgtaaatttc tataaagata tagatatgat agtaagcact tctatttgga aaatggtaaa
     2221 agctccatgg caaaaagaat cggtaattga agcagaatac gcattataac aaacatttgt
     2281 atacacattt taaattttag aaatcatgtt actacgacat acaacagaca aaataataga
     2341 aagggaggct ttgacggtaa atccggctaa aacctgccaa cccgtaggag cgatgtatgc
     2401 agcattgggt attcatggct gcttaccgca tagtcacggg tcgcaaggat gttgttcgta
     2461 ccaccgttcg gtacttacac gtcactacaa agagccggta atggcggcta ccagttcgtt
     2521 taccgaaggt tcgtcggtat ttggtggaca agcaaactta ttg
//
LOCUS       DAOTOW010000295         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31683_length_2554_cov_2.383353, whole genome shotgun sequence.
ACCESSION   DAOTOW010000295 DAOTOW010000000
VERSION     DAOTOW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31683_length_2554_cov_2.383353"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="P5243_06035"
     CDS             complement(<1..581)
                     /locus_tag="P5243_06035"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS19042.1"
                     /translation="MVNYSNLYSGIYKGKKVLITGHTGFKGSWMSVWLDMMGAQVFGY
                     SILEPSEPSHYSCIKMNINEYIADIRNLDTLSEYMKTVQPDIVFHLAAQALVRQSYSD
                     PIETYSTNVMGTLNVLEACRRTASVQAIVIVTSDKCYDNKEWAWGYREIDPMGGFDPY
                     SSSKGCAELLTASYRNSFFNNAESLIAKLIFKVV"
     gene            complement(575..>832)
                     /locus_tag="P5243_06040"
     CDS             complement(575..>832)
                     /locus_tag="P5243_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HRS19043.1"
                     /translation="DFIEKPKGDGNWINGGFFICEPKVLDYIQDGDQSIWERAPLQNL
                     AKDGEMVGYKHEGFWQPMDTLRDKIYLNDLWEKGQAKWKVW"
     assembly_gap    833..842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(843..1338)
                     /locus_tag="P5243_06045"
                     /pseudo
     CDS             complement(843..1338)
                     /locus_tag="P5243_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835541.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            complement(1521..>2554)
                     /locus_tag="P5243_06050"
     CDS             complement(1521..>2554)
                     /locus_tag="P5243_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS19044.1"
                     /translation="EAAKKHGAIWLELMNIPGAKAGKINRALELTNEDYILVLDPDHI
                     PFPNFLDCTLGYFSDPKIGFVQVSQAYYNQYRSLVAQGAAEQTYSFYGPTQMGYYGLD
                     VAIAIGANCTFRRSALQSIGGHAVGLAEDLQTSIKLHAAGWKSVYNPIIVSRGIVPED
                     FDSFCKQQLKWARGTFEVLFEDYPRAFKKLTWKQRFIYLTIATYYFNGFITFLFTLLP
                     VTFIVIGISPVNMQISDFVARGFWIILVAIIFYFYTQRWMCHPTSERGFHWRAMALKY
                     ATWPVFFLGLMLSVVKKEIPYIPTPKNKSTKFSLYARPMQWIIGYVFITITAFIVLRA
                     FIIPLYNIP"
BASE COUNT          814 a          473 c          426 g          831 t
ORIGIN      
        1 acaactttga atattagttt agcaattaac gattctgcat tattaaaaaa tgaattacga
       61 tatgatgcag tgagtaactc ggcacaacct tttgaagagc tgtatgggtc gaacccaccc
      121 atagggtcaa tctctctata tccccaagcc cattctttat tgtcgtagca tttgtcgctg
      181 gtaacaataa caattgcttg taccgatgct gtacgacgac atgcctcaag cacattgaga
      241 gtacccataa cattggtgga gtatgtttcg atagggtctg agtatgattg ccgaactaat
      301 gcctgagccg caagatgaaa tacaatatcg ggctgaactg ttttcatgta ttccgatagt
      361 gtatcaagat tacgaatatc tgcaatgtac tcatttatat tcatttttat acacgaataa
      421 tgagaaggtt ctgatggttc caaaatagaa tacccgaaca cttgtgctcc catcatatcg
      481 agccatactg acatccatga ccccttaaag ccggtatgtc cggtaataag aacttttttc
      541 cctttataaa tacctgaata gaggttgcta taatttacca tactttccat tttgcttgtc
      601 ctttttccca taaatcattt aaatatattt tatctctaag agtatccatt ggttgccaaa
      661 aaccttcatg cttatagcct accatttcac catcttttgc taaattttgt aaaggagctc
      721 gttcccaaat actttgatcg ccatcttgta tataatcaag aacctttggt tcgcaaataa
      781 aaaaaccgcc atttatccaa tttccatcgc cttttggttt ttctataaaa tcnnnnnnnn
      841 nncaattttt aaaccaccaa aacgaccttc gggttgaaca gatgtcattg taacagcttt
      901 gccatgtttt ttatgaaatt ctagtaagtt tgaaatattc acatcactta atccatcgcc
      961 atatgtcaac ataaatcttt cattaccaat gtgttgtttt gcgcgtaaaa ttcggcctcc
     1021 ggtcatggta tttagtcctg tgtctaatag agttaccctc catggctctg catgattatt
     1081 tataatttta atatcattgt ttgagatatc tattgtaata tcactttggt gtagaaaata
     1141 gttagtaaaa tattctttta taacatagct tttataccct aataataata caaaatcatt
     1201 gaagccatag tatgaataaa ttttcataat atgccagaga ataggataac caccaatttg
     1261 aatcatcggt ttcggtttag tatcagtttc ttcactaaat cgtgtgccaa aacctcctgc
     1321 aagaataagt actttcataa tgtaattatt taatagtttt atttacatat tttattgtta
     1381 aagattgaat atatatcaat tatatcccat gggtctttgg gccacttgtg tttgtcagga
     1441 cgagcaacaa aaagcccaat tatagacata atgaacgtaa gcattataaa agcaatcatc
     1501 atccagtctt tttcgagagt ctaaggaata ttgtaaagag gaatgataaa agcgcgtaat
     1561 acgataaaag cagtaattgt tataaaaaca tatcctatga tccattgcat tggacgtgca
     1621 tatagagaaa atttggtgct tttattttta ggggtaggaa tgtacggaat ttcttttttt
     1681 accacagata gcatcaatcc aagaaaaaaa acaggccatg tagcgtattt taatgccatt
     1741 gcacgccaat gaaatccgcg ttccgatgta gggtgacaca tccatcgttg agtatagaaa
     1801 taaaatatga tagcaacaag aataatccaa aagccacgag caacaaagtc acttatttgc
     1861 atatttacgg gagaaattcc aataacaata aatgtaaccg gcaacaaagt aaataagaag
     1921 gtaataaatc cattaaaata ataagtcgca atagttaaat atatgaatcg ttgtttccat
     1981 gttaattttt tgaaagcacg aggatagtct tcaaataaca cttcaaaagt accccgtgcc
     2041 cattttaact gttgtttgca aaatgaatca aaatcttcgg gtacaattcc tcgacttaca
     2101 attatgggat tatataccga tttccaacct gcggcatgaa gttttattga ggtttgtaaa
     2161 tcttcggcaa gtcctacagc atgcccccct atactttgta atgcactacg tcgaaatgta
     2221 caatttgcac ctattgctat ggcaacatca agtccataat atcccatttg agtaggacca
     2281 taaaatgagt atgtttgttc tgctgcccct tgagctacta atgagcgata ttgattatag
     2341 tatgcttgcg atacttgtac gaatcctatt ttaggatctg agaaatatcc aagtgtgcaa
     2401 tcaagaaaat tggggaatgg aatatggtcg gggtcgagga caaggatata atcttcattg
     2461 gttagttcaa gtgcacgatt aatttttccg gcttttgctc cgggaatatt cataagttcc
     2521 agccatatag caccatgttt ttttgctgct tccc
//
LOCUS       DAOTOW010000296         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31730_length_2551_cov_2.480769, whole genome shotgun sequence.
ACCESSION   DAOTOW010000296 DAOTOW010000000
VERSION     DAOTOW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31730_length_2551_cov_2.480769"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="P5243_06055"
     CDS             complement(<1..281)
                     /locus_tag="P5243_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRS19045.1"
                     /translation="MNTIKAAITGVYGWVPEDIITNDDLSKMVDTNDEWITTRTGIKE
                     RRILKDPTLSTSDMAVKAIEGLLKQTNTKPEEIEVIIVATITPDMVFPS"
     gene            complement(281..1222)
                     /gene="plsX"
                     /locus_tag="P5243_06060"
     CDS             complement(281..1222)
                     /gene="plsX"
                     /locus_tag="P5243_06060"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRS19046.1"
                     /translation="MRIGIDAMGGDFAPETAVMGAYLASKELPADITIVLFGNKQAIN
                     TILEREGYSGNSIDIVETTEVIGMGEHPAKAFQQKQDSSITVGFKHLYSGKIDAFASA
                     GNTGAMMVGTMYTIKAIPGIMRPCITGPVPKISGGKTSTLLDVGLNSDCKPDVLYQYG
                     ILGNLFAKYYYGIDNPKVCLLNIGEEEEKGNLLTKAAHELMKEGCHFQFNGNIESNNL
                     YSDQADVIVCDGFTGNILLKQIESLYTLSQKLNVSHSFFEHFNYENHGGTPVLGVNAP
                     VIIGHGASNAKAIKNMILQTYKITLSDLTEKIKQAFQ"
     gene            complement(1247..1438)
                     /gene="rpmF"
                     /locus_tag="P5243_06065"
     CDS             complement(1247..1438)
                     /gene="rpmF"
                     /locus_tag="P5243_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRS19047.1"
                     /translation="MAHPKRKTSKTRRDKRRTHDKATVATLSKCPTTGTIHRRHTAYE
                     VEGNLYYNGKLMIEKKENA"
     gene            complement(1450..1944)
                     /locus_tag="P5243_06070"
     CDS             complement(1450..1944)
                     /locus_tag="P5243_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRS19048.1"
                     /translation="MYKIPIQRLTEGVHTIEIEIEKEFIEQQKIEEMHDIQVHVVVVF
                     TKRLQLHTMQITMRGRVFVPCDRCLETVEIPITNTQTFVVKQALIADELAEADDVILY
                     SQDDLELDISHILYENIVLSLPLKKVHDEKKCNTLITSYISKKNKEQTIDPRWESLKN
                     IFKN"
     gene            2142..>2551
                     /locus_tag="P5243_06075"
     CDS             2142..>2551
                     /locus_tag="P5243_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19049.1"
                     /translation="MKITPSEIYSLYQPVEEKYNNTTEQLPKTIQPLKHLIKQLQEEE
                     DYYSGEQHKTKLMITRLRKIFYDNWGWSSEVIRGATHIPGRYTVSMIKNPNEFAHKKR
                     VGKNQRDDSYNYVGLIPVVVYSKNDKQYPERAGQV"
BASE COUNT          829 a          441 c          365 g          916 t
ORIGIN      
        1 catgaaggaa ataccatatc aggtgttata gttgctacta taatcacttc aatttcttct
       61 ggttttgtat ttgtttgttt caaaagccct tcaatagcct taactgccat atcagaggta
      121 cttaaagtag gatcttttaa aattctgcgt tcttttattc ctgttcttgt tgtaatccat
      181 tcgtcattag tgtcaaccat tttagacaaa tcatcatttg tgataatatc ttcaggtacc
      241 caaccgtata caccggttat agctgcttta attgtattca ttattgaaat gcttgtttaa
      301 ttttctcagt taaatctgaa agggtaattt tataggtttg taaaatcatg tttttaatag
      361 cttttgcatt tgatgctcca tgacctataa taacaggagc atttacacca aggacaggag
      421 tacctccatg attttcataa ttaaaatgtt caaaaaatga atgtgacaca tttaattttt
      481 gagaaagagt atagagagat tctatttgtt ttaataaaat attaccggta aatccatcac
      541 atacaattac atcggcttga tcactatata aattgttact ttcaatattt ccgttaaatt
      601 ggaagtgaca tccttctttc atcagttcat gagcagcctt tgtcaacaaa ttcccttttt
      661 cttcctcttc tccaatattt agtaaacaaa ccttaggatt gtcaattcca tagtaatact
      721 tggcaaataa attaccaaga attccatatt gataaagtac atctggttta caatcagaat
      781 ttaaacctac atctaataat gtagatgttt tacctcctga aattttggga acaggacctg
      841 taatacatgg tctcataatt ccgggaattg ctttaatagt atacatggtt ccaaccatca
      901 tagcacctgt attaccggca ctggcaaatg catcaatttt acctgaatat aaatgtttaa
      961 atcctacagt aatacttgaa tcttgctttt gttgaaacgc tttagcggga tgttctccca
     1021 taccaattac ttctgtagtt tcaacaatat caattgaatt accggaatat ccttcacgtt
     1081 ctaatattgt attaattgct tgcttattac cgaataatac aatagtaata tctgcaggta
     1141 attctttact tgccaaatat gcacccatta cggctgtttc gggagcaaaa tccccgccca
     1201 tagcatcgat tccaattctc atgctatcta ctttaaaatt attagcttac gcgttttctt
     1261 tcttttcaat catcaatttg ccattgtagt acaagtttcc ttctacttca taagctgtat
     1321 gacgacggtg gattgtacct gtagtaggac atttactaag agttgctact gttgctttgt
     1381 cgtgcgtacg tcttttatct ctacgcgttt tcgatgtttt tcgtttagga tgtgccatcg
     1441 ttgttacatt taatttttaa aaatattctt aagtgattcc catcggggat ctattgtttg
     1501 ttctttattt ttttttgaaa tatacgatgt tattaacgta ttacattttt tttcatcgtg
     1561 tactttttta agaggtaatg ataatacaat attttcatat aatatatgag aaatatctaa
     1621 ttccagatca tcttgcgaat ataaaattac atcgtcagct tctgctaatt catcagcaat
     1681 taatgcttgt tttacaacaa atgtttgtgt attcgtaatt ggaatttcta ccgtttccaa
     1741 acatctatcg caaggaacaa atacccgccc acgcatggta atttgcattg tatgcaattg
     1801 taatcgttta gtaaaaacta ctacaacatg cacttgaata tcatgcattt cctcaatttt
     1861 ttgttgttca atgaactcct tttctatttc aatctctatg gtatgtaccc cttctgttaa
     1921 tcgttgaata ggtattttat acatatattg tttattatac ataccttaaa aattagcacg
     1981 caaaattagg aaaaactttt gaattacaaa atttagaatg agtatttcac aaaaaataaa
     2041 aaaaataaag aaaatcattc tttcatgtat acgtaaatgt ttatatttga atttgaaaag
     2101 ttttcatgct gaagaagaaa tactacctat actataatta aatgaaaata actccctctg
     2161 aaatttactc gctgtatcag ccagttgaag aaaagtataa taacacaacc gaacaactcc
     2221 ctaaaactat tcagccatta aaacatctca taaaacaatt acaagaagaa gaagattact
     2281 atagtggcga acaacacaaa acaaaattaa tgataacccg tttacgaaaa attttttatg
     2341 ataattgggg gtggagttct gaagttatac gaggagcaac acatataccg ggtagatata
     2401 ctgtttctat gattaaaaat cctaatgaat ttgcccacaa aaaacgtgtt ggtaaaaatc
     2461 aacgagatga ttcttataat tatgtaggat tgatacctgt agttgtatat agcaaaaatg
     2521 acaaacaata tcccgaacga gcaggtcaag t
//
LOCUS       DAOTOW010000297         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31757_length_2549_cov_3.202085, whole genome shotgun sequence.
ACCESSION   DAOTOW010000297 DAOTOW010000000
VERSION     DAOTOW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31757_length_2549_cov_3.202085"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5243_06080"
     CDS             complement(<1..605)
                     /locus_tag="P5243_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HRS19050.1"
                     /translation="MGKEIKFSLVYRDMWQSAGKYMPRVDQLVRIAPEIINMGCFARV
                     ETNGGGFEQINLLFGENPNKAVREWCKPFNEAGIQTHMLDRSLNGLRMSPVPADVRKL
                     FYKVKKAQGVDITRCFCGLNDPRNIIASIKYSKAAGMIAQATLSLTVSKVHTVEYYCN
                     LADELIAAGADEICLKDMAGIGRPVSLGKIVAHIKKNNPNI"
     gene            complement(644..898)
                     /locus_tag="P5243_06085"
     CDS             complement(644..898)
                     /locus_tag="P5243_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRS19051.1"
                     /translation="MELALLLLVVGMLTVMVALFLVVGVGNMIIRFVNKYVPAEVIVK
                     TKNTVSQTTTAIPANTMAAIVSAVSMVTGGRGNVVDVKKV"
     gene            1152..2087
                     /locus_tag="P5243_06090"
     CDS             1152..2087
                     /locus_tag="P5243_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS19052.1"
                     /translation="MTGISIQNITKRFSHTVALNSVSLEIPQGSIYGLLGPNGAGKTT
                     CIRILTQILQPDSGTITLYNKNLEPDDITKFGYLPEERGLYKQMYVGEQIMYFASLKG
                     MSKIEAKQECNFWLQKFNIEQWKHKKAGELSKGMQQKIQFICAIIHKPEILILDEPFS
                     GFDPINADIIKQEILQLKKLGTTIILSTHNMNSVEELCDSIALINASEKIIEGSVSAI
                     KKQFAKNIYSITFSGYYDKFLATLTTSYKILEQIQNNGETTILIELTEEIPTNNLLTH
                     IMTCGNIIDFSQIIPNMHDIFIDVVKNSQSTKLHE"
     gene            2080..>2549
                     /locus_tag="P5243_06095"
     CDS             2080..>2549
                     /locus_tag="P5243_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19053.1"
                     /translation="MNKIRLIFIRECSVRLRKRSYILMSILGPLLLAAIVVIPLLLKK
                     IDKNTVKQVAIIDETYILGETIKNFENYRFTIIQDTTLEDFRKHYKASGYDAVVFIPK
                     NIYSSNACIIYSHVWIDNALKAYIGYVLRRDLEYMALLKEKVTIETIKRVSTPV"
BASE COUNT          944 a          417 c          342 g          846 t
ORIGIN      
        1 attatatttg gattgttctt ttttatatga gcaactattt ttcctaatga aacaggtcga
       61 ccaatacctg ccatatcttt caaacaaatt tcgtctgcac ctgcagcaat taactcatct
      121 gccaaattac aatagtattc tactgtatgt actttagaaa cagtaagcga caaagtagct
      181 tgtgctatca tgcctgctgc tttagaatat ttaatagatg cgataatatt gcgaggatcg
      241 ttcaacccac aaaaacaacg agtaatatct acaccttgtg ctttttttac tttataaaat
      301 aatttacgaa catcggcagg aacaggactc atacgtaaac cattcaatga acgatcaagc
      361 atatgtgttt gaatacctgc ttcgttgaat ggcttacacc attcacgaac tgctttattt
      421 ggattttcac caaataataa attaatttgt tcaaaaccgc caccgttagt ttcaacacgt
      481 gcaaaacaac ccatattaat aatttcggga gctatacgca ccaactgatc tacacgcggc
      541 atatactttc cggcactttg ccacatgtct ctgtaaacta acgaaaattt aatttctttt
      601 cccatataca tgaatttaat taatttttta cttttttatg tttttatact ttttttacat
      661 ctactacatt acctcttcct cctgttacca tagaaactgc agaaacaatt gcagccatag
      721 tattggcagg tattgctgtt gtagtttgcg aaacggtatt ttttgttttt acaataactt
      781 cggcaggaac atatttgttg acaaatctaa taatcatatt tcctactcca acaactaaaa
      841 agagtgctac cattacggta agcataccta ctactaataa caataatgct aattccattt
      901 atctcgtttt ttatgtttta aaaattgggc gaaagttact cattaaaatt gaattatgaa
      961 aacgttatat taaaatacac tgcttatttt attcaaattc atgataaaaa aatataatct
     1021 aaatcatatt aaaataatgt aaaattatta atatctatat ttaattttga ttacaatact
     1081 aaaaacattt atttaacatt attgtgtttt ttatcacaca ataataatat ttttgtaaaa
     1141 aattaatagg aatgaccgga atttcaattc aaaacattac aaaacgattt tcgcacacag
     1201 tagcacttaa ttctgtatcg ctcgaaatac cgcaaggttc tatatacgga ctattgggac
     1261 ctaatggtgc aggaaaaact acatgtatta gaattcttac acaaatatta caaccggatt
     1321 ccggcacaat tacactatat aataaaaatc ttgagccaga tgatattaca aaatttggtt
     1381 atttacccga agaacgtggg ttatacaaac aaatgtatgt aggcgaacaa attatgtatt
     1441 ttgcttcact caaaggtatg agtaaaattg aagcaaaaca agaatgtaat ttttggctgc
     1501 aaaaatttaa tatagaacaa tggaaacaca aaaaagccgg agaattatca aaaggtatgc
     1561 aacaaaaaat tcaatttatt tgtgctatca tacataaacc ggaaatactt atacttgatg
     1621 aacctttcag tggatttgac cctataaacg ccgatattat taaacaggaa atattacaat
     1681 taaaaaaact aggaactaca attattttat ctacacacaa catgaattct gttgaagaat
     1741 tgtgtgattc tattgcttta attaatgctt cagaaaaaat tattgaaggc agtgtatctg
     1801 ctataaaaaa acaatttgca aaaaatatat attctattac ttttagtgga tattacgata
     1861 aatttttagc tactctaacc acatcataca agattttgga acaaattcaa aataacggag
     1921 aaactacaat actaatagaa ttaaccgaag aaatacctac taataattta cttactcata
     1981 tcatgacctg tggtaatatt attgattttt cacaaattat tcccaatatg catgatattt
     2041 ttattgacgt ggtaaaaaac tctcaatcaa caaaactcca tgaataaaat acgtttaata
     2101 tttataagag aatgctctgt acgacttcgc aaacggtcat atatacttat gagtatatta
     2161 ggtccactat tattggctgc tatagttgta atacctctgt tactcaaaaa aattgacaaa
     2221 aatacggtta aacaagttgc aataattgat gaaacataca tattgggaga aactataaaa
     2281 aactttgaaa attatagatt tacaattatt caagatacta ccctagaaga ttttagaaaa
     2341 cattataaag cttcggggta tgatgctgtg gtgtttattc caaaaaacat ttatagttcc
     2401 aatgcctgta ttatttactc gcatgtatgg atagataatg cacttaaagc atatattgga
     2461 tatgttctgc gaagagattt agaatatatg gcacttctta aagaaaaagt taccatagaa
     2521 actataaaac gagtatcaac accggtatt
//
LOCUS       DAOTOW010000298         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31792_length_2546_cov_3.323163, whole genome shotgun sequence.
ACCESSION   DAOTOW010000298 DAOTOW010000000
VERSION     DAOTOW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31792_length_2546_cov_3.323163"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1947
                     /locus_tag="P5243_06100"
     CDS             301..1947
                     /locus_tag="P5243_06100"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HRS19054.1"
                     /translation="MKRNLIYSFVIVIVGIVLFSSCSPMPKHAQYIPSDAAFVSVIDI
                     VSIAQKGKLQDAKSLKMYSQAIEFFNSTNQNTGILLESLVTEPKKTGIDIFSDLFVYS
                     VIQNEKVYMCVSVKLDNKENFQNLMEGILGDEFDLQEQDAFTYYTNESNTWIAFNDKN
                     AVFISSGQAKSDEALELIQRIFTQSKDESIAESKEFIEFYKQKKDVSIWISSTHIIEG
                     MSNETKKAIEKNLESFQGLSINDILNNYIHLFLNFEEDDITLDFNLQANDAFKTFLDK
                     TDFTKDKLSEEVLSFLPQTSYFLMSYAFESDKLVQYLQSLPDFKQLDDEIQNQGVSLK
                     EFISALNGDIVLSVFNASLDVVQKNKTLLRQDSKGRYQYVDTVIYDEQIIPKAAMVLS
                     LKDEEYMKKFIAEKIPAELYTKQDSYLDFTKTLNFPLYLGIHNNMLMLSTDIGAMSQL
                     YDGGYPESLSTSDLVQEIDAAAFYYVNLDFETYPIQVKEFVNKSFLQHILKPYLNTFE
                     SLTISSNGLYSGRLQIKLKNSKENSLYQVLEMLDGNFKQQ"
     gene            1962..>2546
                     /locus_tag="P5243_06105"
     CDS             1962..>2546
                     /locus_tag="P5243_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS19055.1"
                     /translation="MNISFCNVVPMPMKEVLGNSHIWQQNIQIQAGSCNQIVAPSGTG
                     KTSLIHFLYGIRSDFEGVIEFDSTSIQSFSVKQWSQYRTQKISIVFQGLRLFAELDVM
                     ENIQIKNKRTNYKTVSQIHQLLDAVGMMPHIHKKAGILSYGQQQRVAIVRALCQPFEL
                     LLLDEPFSHLDNMNAQAAFECIYNEVQLQQATIVL"
BASE COUNT          943 a          318 c          410 g          875 t
ORIGIN      
        1 aagaatatat acaaaaagcc ggtaaagaga tacttctttt aatagaaaag cgaaaaaaac
       61 ctatattaga gaaaaaagta ttatcaaaag ctaaaggtgg gtgttaaata ttttttagat
      121 tgttgtatga ttagaatatt ttgaattcct ggaaacacat cttagattaa ttcaatattt
      181 caacgtattt tttctgtctt atttttcaac aaaaaattat gtaataattc tgtaagtttt
      241 cctttagaga aaactaaaaa tattattttt gtacaataat aacaagtaat tattttttgt
      301 atgaaacgta atttgatata ttcattcgta attgtaatag ttggtatagt attgtttagt
      361 tcgtgttctc ctatgccaaa acatgctcag tatattcctt ctgatgcagc atttgtaagt
      421 gtgattgata ttgtaagtat tgctcaaaaa ggaaaattgc aagatgcaaa atcgctaaag
      481 atgtattcac aggctattga attttttaac tctacaaatc aaaatacggg tattctatta
      541 gaatcattag ttactgaacc taaaaaaacc ggaatagata ttttttctga tttgtttgtg
      601 tattctgtaa ttcaaaatga aaaagtatat atgtgtgtgt ctgtaaaact tgataataaa
      661 gaaaattttc aaaacttaat ggagggaatt ttgggtgatg aatttgattt gcaagaacaa
      721 gatgcattta cttattatac caatgaatct aatacatgga ttgcttttaa tgataaaaat
      781 gctgtattta tttcttcggg tcaggcaaaa tcagatgaag ctttggaatt aatacaaaga
      841 atttttacac aatcaaaaga tgaatcaata gctgaaagca aagaatttat tgaattttat
      901 aaacaaaaaa aagatgttag tatatggatt tcttctacac atataataga aggaatgtca
      961 aatgaaacaa aaaaagcaat tgaaaaaaat ttagaatctt ttcaaggtct ttctataaat
     1021 gatattttaa ataattatat tcatttgttc ctcaattttg aagaagatga cataactttg
     1081 gattttaatc tacaagcaaa tgatgctttt aaaacatttt tagataaaac tgatttcaca
     1141 aaagataaac tttctgaaga ggtattatct tttttaccac aaacaagcta ttttttaatg
     1201 agttatgcat ttgaaagtga taaattagtt cagtatttgc aaagcttacc cgattttaaa
     1261 caattagatg atgaaataca aaatcaagga gtatcactta aagaatttat atcggcatta
     1321 aatggagata tagtattaag tgtgtttaat gcatcgcttg atgttgttca aaaaaataaa
     1381 acattgttac gccaagattc taaaggaaga tatcaatatg tggatacagt aatttatgac
     1441 gagcaaatca ttccaaaagc agcaatggtt ttgagtttaa aagatgaaga gtatatgaaa
     1501 aaatttattg ccgaaaaaat tcctgcagag ttatatacca aacaagattc ttatttagat
     1561 tttacaaaaa cgcttaattt tccattgtat ttgggtattc ataataatat gctcatgcta
     1621 agtaccgata tcggtgcaat gagtcaattg tacgatggtg gttatcctga atcgttaagt
     1681 acatctgatt tggtgcaaga aatagatgct gcggcatttt attatgtgaa tttggatttt
     1741 gaaacatatc ctattcaagt aaaagaattt gttaataaat catttttaca acatatctta
     1801 aaaccatatc ttaatacgtt tgaatcacta actatttcat caaatggttt atacagcggc
     1861 agattacaaa ttaaattaaa aaacagtaaa gaaaatagtt tgtatcaggt gttggaaatg
     1921 cttgatggaa atttcaagca acaataatta ctgtatgctt tatgaatatt tcgttttgca
     1981 atgtagttcc tatgcctatg aaagaggtgc ttggcaactc acacatatgg cagcaaaata
     2041 tacaaattca agcaggttca tgtaatcaaa tagtagcacc gagcggcacc ggaaaaactt
     2101 cattaattca ttttttatat ggtattcgca gtgattttga aggtgtaata gaatttgatt
     2161 ctacttcaat acaatcattc tcagttaaac aatggagtca atacagaact caaaagatta
     2221 gtattgtttt tcaaggatta cgattatttg cagagcttga tgttatggaa aacattcaaa
     2281 ttaaaaataa gcgtaccaat tataaaacag tatctcaaat acatcaattg ctggatgctg
     2341 ttggtatgat gcctcatata cataaaaaag caggtatatt atcgtatggt caacagcagc
     2401 gtgttgcaat agtgagagct ttgtgtcaac catttgagtt gttgttactc gatgaaccgt
     2461 ttagtcatct tgacaacatg aatgctcagg ctgcttttga gtgtatatac aatgaagtac
     2521 aattgcaaca agctaccatt gtgctt
//
LOCUS       DAOTOW010000299         2540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31871_length_2540_cov_4.210463, whole genome shotgun sequence.
ACCESSION   DAOTOW010000299 DAOTOW010000000
VERSION     DAOTOW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31871_length_2540_cov_4.210463"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="P5243_06110"
     CDS             <1..142
                     /locus_tag="P5243_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HRS19056.1"
                     /translation="APVTPGRILFEAEGVPLDVAKEALRLAAQKLPVTTKFIVRNDYV
                     QE"
     gene            157..351
                     /gene="rpmC"
                     /locus_tag="P5243_06115"
     CDS             157..351
                     /gene="rpmC"
                     /locus_tag="P5243_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRS19057.1"
                     /translation="MKAAEIRELTTKELEEKLQVELEQINKMKINHAISPLENPNVIK
                     ESRKKIARLQTILKERELNK"
     gene            369..623
                     /gene="rpsQ"
                     /locus_tag="P5243_06120"
     CDS             369..623
                     /gene="rpsQ"
                     /locus_tag="P5243_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRS19058.1"
                     /translation="MERNFRKERIGIVVSNKMEKSIVVAVERKVKHPMYGKFLKKTTK
                     FVAHDEKNECNEGDTVRIMETRPLSKSKCWRLVEIIERAK"
     gene            625..993
                     /gene="rplN"
                     /locus_tag="P5243_06125"
     CDS             625..993
                     /gene="rplN"
                     /locus_tag="P5243_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRS19059.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGGTRKRYASIGDKIVVTVK
                     DAIPNGNVRKGQVTKGVIVRTTKEVKRPDGSYIRFDDNAVVLLNEAGEMRGTRIFGPV
                     GRELRDKQYMKIVSLAPEVL"
     gene            1011..1334
                     /gene="rplX"
                     /locus_tag="P5243_06130"
     CDS             1011..1334
                     /gene="rplX"
                     /locus_tag="P5243_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRS19060.1"
                     /translation="MQKKYHIKKGDTVVVITGEYKGTEGKVLEMITKTDKAIVEGVNL
                     ISKATKPNAKNPQGGIVKKEAPIHISNLMLVNPATGKPTKVGRKVNEKGKLVRYSKKT
                     GEEIK"
     gene            1334..1879
                     /gene="rplE"
                     /locus_tag="P5243_06135"
     CDS             1334..1879
                     /gene="rplE"
                     /locus_tag="P5243_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRS19061.1"
                     /translation="MSYTPTLKKLYFEKVQGNLQKEYGYKSKMQVPRIEKIVINQGIG
                     KAVADKKLIETAVNELSLIAGQKAIPTYSKKDISNFKLRKGMPIGVKVTLRREKMYEF
                     LERLISAALPRIRDFQGFNTKFDKRGNYTFGISEQIIFPEIEVDKVNTMLGMDITIVT
                     TAKTDEEGFALLKEFGFPFKK"
     gene            1886..2155
                     /gene="rpsN"
                     /locus_tag="P5243_06140"
     CDS             1886..2155
                     /gene="rpsN"
                     /locus_tag="P5243_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRS19062.1"
                     /translation="MAKESMKAREVKRKKLVDKYAKKRAELKAKGDYIGLSKLPKNSN
                     PNRLHNRCNITGRPKGYMRQFGISRIQFREMASAGLIPGVKKASW"
     gene            2173..>2540
                     /gene="rpsH"
                     /locus_tag="P5243_06145"
     CDS             2173..>2540
                     /gene="rpsH"
                     /locus_tag="P5243_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRS19063.1"
                     /translation="MTDPIADYLTRIRNAQAARHKVVEIPSSNIKKEITKVLHDKGYI
                     LSYKFDDSADVQGVIKIALKYHPVSKSPAIKSLQRVSKPCLRKYVSTDEMPRVLNGLG
                     IAILSTNKGIVSDKEAKALNV"
BASE COUNT         1065 a          305 c          477 g          693 t
ORIGIN      
        1 tgctccggtt acaccgggta gaattttatt tgaagctgaa ggagttcctt tagatgttgc
       61 taaagaagcg cttcgtttgg ctgctcaaaa attgcctgta acaacaaagt ttattgttcg
      121 taacgattat gttcaagaat agaaaaaatg atacacatga aagcagctga aataagagaa
      181 ttaacaacaa aagaattgga agaaaaacta caagttgaat tagagcagat taataaaatg
      241 aaaattaatc atgcaatatc accacttgaa aatccgaatg taataaaaga atctcgtaag
      301 aaaattgctc gattacaaac aattttaaaa gaacgagaac ttaataaata agataagtaa
      361 tatctaacat ggaaagaaat ttcagaaaag aacgtatagg aatcgttgtg agcaacaaaa
      421 tggaaaaatc aattgttgtt gctgtagaga gaaaagttaa acaccctatg tatggtaagt
      481 ttttaaaaaa aacaaccaaa tttgtagctc atgacgaaaa aaatgagtgt aatgagggtg
      541 atactgttag aataatggaa acacgcccat taagtaaaag caaatgttgg cgattagtag
      601 aaatcataga aagagcgaaa taagatgata cagcaagaat caagattaaa tgttgcagat
      661 aacagcggag caaaagaagt tctctgtata cgagtactag gtggaactcg taaaagatat
      721 gcatctatag gcgataaaat tgttgttacc gtaaaagatg ctataccaaa tggtaacgta
      781 agaaaaggac aggttactaa aggagtaatt gttcgtacaa caaaagaagt taaaagacct
      841 gatggttcat atattcgttt cgatgataat gctgttgttt tattaaacga agcaggagaa
      901 atgagaggta ctcgtatatt tggaccggtt ggtcgtgaat tacgtgataa acaatatatg
      961 aaaattgttt cattagcacc tgaagtatta taaataaaac aataattaag atgcaaaaaa
     1021 aatatcatat aaaaaagggt gatacagtag tggttattac cggagaatat aaaggaaccg
     1081 aaggaaaagt tttagaaatg attactaaaa ctgataaagc tattgtggaa ggagtaaatt
     1141 tgatttcaaa agcaacaaaa cctaatgcta aaaatccgca aggtggaatt gtaaaaaaag
     1201 aggctccaat tcatatatca aatctaatgc ttgtaaatcc tgcaacaggt aaaccaacaa
     1261 aagttggcag aaaagtgaat gaaaaaggaa aattagttag gtattcaaaa aagacaggag
     1321 aggagattaa ataatgagct atacacctac attaaaaaag ttatatttcg aaaaagttca
     1381 aggaaactta caaaaggaat atgggtataa atcaaaaatg caagtacctc gaattgagaa
     1441 aattgttata aatcaaggaa taggtaaagc tgttgccgat aaaaaattaa ttgaaactgc
     1501 agtaaatgaa ttatctttga ttgcaggtca aaaagcaata ccaacttatt ctaaaaagga
     1561 tatatcaaat ttcaaattac gtaaaggtat gccaataggt gttaaagtaa ctttacgaag
     1621 agaaaaaatg tatgaatttc ttgaacgatt aatttctgca gctttgcctc gaatcagaga
     1681 ttttcaagga tttaatacaa aatttgataa acgtggtaat tatacatttg gaatctctga
     1741 acaaattatt tttcctgaaa ttgaggttga taaagttaat acaatgttag gaatggatat
     1801 tactatagtt actactgcaa aaactgatga agaaggtttt gcattattaa aagaatttgg
     1861 ttttccattt aaaaaataag aaattatggc aaaagagtca atgaaagctc gcgaagttaa
     1921 gcgtaaaaaa ttagttgata aatatgcgaa aaagcgcgca gaattaaaag caaaaggtga
     1981 ttatataggt ttaagtaaat tacctaaaaa ttcaaatcct aatagattgc ataatagatg
     2041 taatattaca ggtcgtccga aaggatatat gagacaattt ggaattagtc gtatccaatt
     2101 tagagaaatg gcatcggcag gattaattcc gggagttaaa aaagcaagtt ggtaaaataa
     2161 tataaaagag acatgacaga tccaatagca gattatctaa caagaattag aaatgctcaa
     2221 gcagcacgtc ataaagtagt tgagattcct tcttcaaata ttaaaaagga aattactaaa
     2281 gtacttcatg acaaaggata tattttgagt tataaatttg atgattcagc agatgtgcaa
     2341 ggagttataa aaatagcttt aaaatatcat cctgtatcaa aatcaccggc aattaaatcg
     2401 ttacaacgtg tgagtaaacc atgtttaaga aaatatgttt caacagatga aatgcctcgt
     2461 gtattaaatg gtttaggaat agctattctt tctacaaata aaggaatagt ttcagacaaa
     2521 gaagccaaag cattgaatgt
//
LOCUS       DAOTOW010000300         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_31940_length_2537_cov_2.549154, whole genome shotgun sequence.
ACCESSION   DAOTOW010000300 DAOTOW010000000
VERSION     DAOTOW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31940_length_2537_cov_2.549154"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="P5243_06150"
     CDS             complement(<1..823)
                     /locus_tag="P5243_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19064.1"
                     /translation="MYLYTRIIYLTIVILWTTLAGTYAQCSAKIGFEPGSSPTGCGYP
                     LAIHFNSTGSTGVQELNWNFGDGKTSFTNNPIHEFYYDSVQKKDALYKVVLSVKCLNG
                     STVKDSVTVTALKIPQVSFTPDTTVACQLGTPVCFTNTSEYDPTYSYLWNFDLGETST
                     DYTPCHKFSLSGFKKPRLTVTTSNSCVNEYTYPGTIEIISVPNPSFIASPQTGCNPMK
                     VTVTNNSNPAQVNKVYWMWGDGQIDSVMHPGNHTYYEAGVYNLKLKIINQFGCENT"
     gene            1062..1910
                     /locus_tag="P5243_06155"
     CDS             1062..1910
                     /locus_tag="P5243_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19065.1"
                     /translation="MKIRLLGIVCIALSTLLFSSCGGSSNDEESILFDSLVNTNKTAK
                     VPPEVLQKILKSLPSPVEMTSIIHQSKAKFNKDLLNPREKFSDYTSSYKKAVNLGIYG
                     TDLGYMNIYEETILSMTYLDAIKSLANDLKIGHFFNMDVIGRMVSNKNNIDSLMYIST
                     SSFDKMSTYLNDQNRAHISSLILLGGWIEGLYLYTQTAKAYPTQQLKDRICEQKIALN
                     DIMVLLSVYENNPTFKELITDITTIKKLYDNVTISYKPGEPKVEIVDGMLQIIDSTES
                     VIEISD"
     gene            1991..2425
                     /locus_tag="P5243_06160"
     CDS             1991..2425
                     /locus_tag="P5243_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19066.1"
                     /translation="MKPYTYILTIALLLPFVSIQAQCESDAFLDNCVELIDNYTYVKV
                     FNVNTDKKTTKVEYSSVFSKDHEYILAICSGTQKSNKKLIVNLYDRNRQLISSSYYKP
                     KNMHVQKVGYKCSATGVYYIEAYFEDNAENCGIIILGFKKSQ"
BASE COUNT          921 a          331 c          399 g          886 t
ORIGIN      
        1 atgtgttttc acacccaaat tggtttataa tttttaattt taaattataa acaccggctt
       61 cgtaatatgt atgattaccg ggatgcatta cagaatctat ttggccgtcg ccccacatcc
      121 aataaacttt atttacttgt gccggattag aattgtttgt tacagtaact ttcataggat
      181 tacacccggt ttgtggtgaa gctataaatg aaggattagg aactgaaata atttcaatgg
      241 ttcccggata tgtatattcg tttacacatg aatttgaagt tgtaacggtt aagcgaggtt
      301 ttttaaatcc cgatagagaa aatttatgac atggtgtata atcagtagag gtttcaccta
      361 agtcaaaatt ccataaatag gaatatgtgg ggtcatattc agaagtattg gtaaaacata
      421 ccggtgtgcc aagttgacaa gcaactgtag tatccggagt aaatgatact tgaggaattt
      481 tgagagctgt aacagttact gaatctttta ctgtagatcc atttaaacat tttacgctta
      541 acacaacttt atataaagca tctttttttt gaacagaatc ataatagaat tcatgaattg
      601 gattgtttgt aaatgatgtt ttaccgtcgc caaaattcca atttagttct tgaacccctg
      661 tacttcccgt actattaaag tgtattgcta atggataccc gcatcctgtt ggtgaacttc
      721 cgggttcaaa accaattttt gctgaacatt gtgcatatgt accagctaat gtagtccata
      781 atattactat agttagatat ataatcctag tatacaaata cataaggtat aatgcaaaag
      841 ttggtctttt gataacaaat atatactttt ttacaagtat ttatactata tttttatcgg
      901 taagtttcga gtgattgatg atataaatca ttgaaaaaaa ttatttaaaa tacaaaccat
      961 tgcttagtaa gtttcgtatt tttacaatgt tgcattttgt ataaaaaaat gataccatta
     1021 aaaatctaaa atatagtaat tacatttaaa ttgtattgaa tatgaaaatt cggttactcg
     1081 gtattgtatg tattgcatta agcacacttc tattttcaag ttgtggcggt agttcaaacg
     1141 atgaagaaag tatattgttt gattcactag taaatactaa taaaacagca aaggttccac
     1201 cggaggtact tcaaaaaatt ttaaaatcat tgccgtcacc tgtcgaaatg acttcaataa
     1261 ttcatcagtc taaagctaag tttaataaag atttattaaa tcctcgtgaa aaattttcag
     1321 attatacttc atcgtataaa aaagcggtga atttgggtat atacggtacc gatttggggt
     1381 atatgaatat ttatgaagaa acaatcctct caatgacata tttagatgct attaaatctt
     1441 tagcaaatga tttaaaaata ggtcattttt ttaatatgga tgtaattggc agaatggttt
     1501 ctaataaaaa caatatagat tcattaatgt atatttcaac ttctagtttt gataaaatga
     1561 gtacctattt gaacgatcaa aatagagctc atattagttc tcttatactt ttgggagggt
     1621 ggatagaagg tttatatttg tatacacaaa cagcaaaagc atatccaact caacaattaa
     1681 aagatagaat ttgcgaacaa aaaatagcat taaatgatat aatggttcta ttaagtgttt
     1741 atgaaaataa tccaacgttt aaagaattaa taaccgatat tacaactatt aaaaaattgt
     1801 atgacaatgt aacaatatca tataaaccgg gagaaccaaa agtagaaatt gtagacggta
     1861 tgttgcaaat aattgattca accgaaagtg ttattgaaat aagtgattaa acttataatt
     1921 ctttgatttc agaggttgca attattcgaa ataaactaat tcaaaaataa cttgttaatt
     1981 ttacataacg atgaaaccat atacatatat cttaaccata gctttattac tgccttttgt
     2041 aagtattcaa gcacaatgcg aaagtgacgc atttttggat aattgcgttg aattaattga
     2101 taattatacc tatgtgaaag tatttaatgt aaatacagat aaaaaaacta ctaaagttga
     2161 atattcttca gtatttagca aagatcatga atatattttg gctatatgca gcgggacaca
     2221 aaaatcgaat aaaaaactga ttgtaaattt atatgaccgt aatcgccagc ttatttcgag
     2281 cagttattat aaacctaaaa atatgcatgt gcaaaaagtg gggtataaat gttctgctac
     2341 cggagtatac tatattgaag catattttga agataatgcc gaaaattgcg gtatcataat
     2401 tttaggattt aaaaaatcgc aataactaaa actttttgtt ttaaaactat gttttgctta
     2461 atataatttg tatttttgat tgtctattgc aattattgaa attgtaaaaa gtatttttaa
     2521 tattttaata attaagt
//
LOCUS       DAOTOW010000301         2527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32104_length_2527_cov_3.272654, whole genome shotgun sequence.
ACCESSION   DAOTOW010000301 DAOTOW010000000
VERSION     DAOTOW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32104_length_2527_cov_3.272654"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2527
                     /locus_tag="P5243_06165"
     CDS             <1..>2527
                     /locus_tag="P5243_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRS19067.1"
                     /translation="IVWDILENVLKGHPVLLNRAPTLHRLGIQAFQPKMIEGKAIQLH
                     PLVCTAFNADFDGDQMAVHLPLGNAAILEAQLLMLSSHNILNPANGAPIMVPSQDMVL
                     GLYYITKERKGAKGEGSIFYSAEEAIIAYNEQKVELHAIVKVLRKDIEGKKSIIDTTV
                     GRIIFNEIVPLEVGYINEILTKKSLREIIGNVLKVAGTAKAAKFLDEMKNLGYSMAFK
                     GGLSFNLSDVIIPKEKEELVNAGYQQVEEVLANYNMGFITNNERYNQIIDIWTHTNAS
                     LTNIVMKQISSDKQGFNSVYMMLDSGARGSKEQIRQLSGMRGLMAKPQKSGSTGNEII
                     ENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVSQDVVITQED
                     CGTLRGLIATSIKNNDEVVETLYDRILGRTCVHDIYHPQTGNLIIASGEEITEGIAQI
                     IEDSPIEQVEIRSVLTCESRKGVCAKCYGRNLATARMVQKGEAVGVIAAQSIGEPGTQ
                     LTLRTFHVGGTASNIAAESSIVSKYDGIVEIDELRTVVSEDETGKPVNIVIGRLGELK
                     VVDKNTGIVLTSQNIPYGSKLFISPGDVVEKGTLLCSWDPYNASIISESAGKIAFENV
                     IEGVTFKKETDEQTGFEEKVIVEAKDKTKNPVIKILDKAGDTIHNYNLPVGAHISVNE
                     GDKVKAGQILVKIPRAIGKSSDITGGLPRVTELFEARNPSNPAIVSEIDGEISFGKIK
                     RGNREISVTSKTGEVKKYLVPLSKQILVQENDYVKAGTCLSDGAITPSDILAIKGPTK
                     VQEYIVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVEIIDPGDS"
BASE COUNT          917 a          371 c          473 g          766 t
ORIGIN      
        1 tatagtttgg gatattttag aaaatgtatt aaaaggtcat cctgtattat taaaccgtgc
       61 tcctactctt caccgtttag gtattcaggc ctttcaacca aaaatgattg aaggaaaagc
      121 aatacaatta catcctttag tatgtactgc attcaatgcc gattttgatg gtgaccaaat
      181 ggctgtccat ttaccattag gaaatgcagc aattcttgag gctcaattat taatgctttc
      241 atctcacaat attctaaacc ctgcaaatgg agcacctatc atggttccat cacaagacat
      301 ggttctaggt ttatattata taacaaaaga acgcaaagga gctaaaggtg aaggttctat
      361 tttttattca gctgaagaag ctataatagc ttataatgaa caaaaagtag aattacatgc
      421 tattgtaaaa gttttacgta aagatataga aggcaaaaaa tcaataattg atacaactgt
      481 aggaagaata atctttaacg aaattgtacc tcttgaagtt ggatatatta atgaaatttt
      541 aactaaaaaa tcattacgtg aaattattgg aaatgtatta aaagttgccg gaacagcaaa
      601 agctgctaaa ttccttgatg aaatgaaaaa tttaggatat agcatggctt tcaaaggcgg
      661 tctttcattt aatctttctg acgtaatcat tccaaaagaa aaagaagaat tagttaatgc
      721 cggatatcaa caagtagaag aagtattagc taattacaac atgggtttta ttactaataa
      781 tgaacggtat aatcaaatta ttgatatttg gacacataca aatgcaagtt tgacaaatat
      841 tgtaatgaaa caaattagct cagataaaca agggttcaat tctgtttata tgatgcttga
      901 ttctggagct cgtggatcta aagaacaaat tcgtcagtta tcgggaatgc gtggattgat
      961 ggcaaaacca caaaaatcag gttctaccgg taacgaaatt attgaaaatc caattctttc
     1021 taactttaaa gaaggacttt cggtattaga atattttatt tctactcacg gtgctcgtaa
     1081 aggtttggcc gatacagctt taaaaacagc agatgcaggt tatttgactc gtcgtttagt
     1141 tgatgtttct caagatgttg taataacaca agaagattgt ggaacattac gtggtcttat
     1201 agcaacatca atcaaaaaca acgacgaagt agttgaaact ttatatgata gaattcttgg
     1261 tagaacatgt gttcacgata tttatcatcc gcaaaccgga aatctgataa tagcttccgg
     1321 tgaagaaata accgaaggaa ttgctcaaat tatagaagat tctcctattg aacaagtaga
     1381 aattcgttct gtacttacat gtgaatcacg aaaaggtgta tgtgcaaaat gttatggtag
     1441 aaacttagcg actgctcgaa tggttcaaaa aggagaagct gttggagtaa tagctgctca
     1501 atcaattggt gaaccgggta cacagcttac acttcgtact ttccacgtgg gtggtactgc
     1561 ttctaacatt gcagcagaat cttctattgt atctaaatac gatggtatag ttgaaataga
     1621 tgaattacga actgttgtat ccgaagatga aaccggcaaa ccggttaata ttgtaattgg
     1681 tcgtttaggt gaattaaaag ttgttgataa aaatacagga attgtattaa catcgcaaaa
     1741 tattccttat ggctctaaat tatttattag cccgggtgat gttgttgaaa aaggcactct
     1801 attatgtagt tgggatccat ataatgcttc aataatttct gaatcggcag gtaaaattgc
     1861 atttgaaaat gtaatagaag gtgttacttt caaaaaagaa acagatgaac aaaccggttt
     1921 tgaagaaaaa gtaattgtag aagcaaaaga taaaactaaa aaccctgtaa ttaaaattct
     1981 tgataaagca ggtgatacaa ttcataacta taacctacct gttggtgctc acatttcagt
     2041 aaacgaaggc gataaagtta aagcaggtca aattcttgta aaaattcctc gtgctattgg
     2101 aaaatcaagt gacattaccg gtggattacc acgtgtaaca gaactctttg aagcacgaaa
     2161 tccttcaaat ccggctatag tatctgaaat tgacggagaa atttcatttg gtaagattaa
     2221 acgaggtaac agagaaattt ctgtaacatc aaaaaccggt gaagttaaaa aatatcttgt
     2281 acctctttca aaacaaatat tggttcaaga aaatgattat gttaaagcag gtacttgttt
     2341 atcagatggc gcaataacac cttcagatat tctggctatt aaaggtccta ctaaagtaca
     2401 ggaatatata gttaatgaag tacaagaagt ttatcgttta caaggtgtaa aaattaatga
     2461 taaacacttt gaggtaattg tacgtcaaat gatgcgtaaa gtagaaataa ttgatccggg
     2521 agatagt
//
LOCUS       DAOTOW010000302         2527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32108_length_2527_cov_2.498786, whole genome shotgun sequence.
ACCESSION   DAOTOW010000302 DAOTOW010000000
VERSION     DAOTOW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32108_length_2527_cov_2.498786"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="P5243_06170"
     CDS             <1..905
                     /locus_tag="P5243_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRS19068.1"
                     /translation="VCVSRCTFCNFHKLIHHDGTYITELEEYSPKIKELEAYGGNQLL
                     IQGGMHPQLGIDFYVQLFSDLKQRHPNIKLHALGPPEIAYIAQKEHMTHKEVLEKLIE
                     SGMDSLPGAGAEILQDHIRKQLSPGKCNTQTWLDVMHQAHILGIVTSATMMYGHIETI
                     ADRMQHLVLLRELQNSKPIHSKGFTAFIPWPFYSKNTVLARQTRKEYAIQHLEYIRMI
                     AISRIMLTNIDNIQASWLTVGIPTAQICLHAGAHDLGSIMIEENVVSQAGAMFTAKKE
                     ELQQAIIQAGFTPKLRNQGYDIIE"
     gene            1028..1369
                     /locus_tag="P5243_06175"
     CDS             1028..1369
                     /locus_tag="P5243_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HRS19069.1"
                     /translation="MSEELKQCPQCASPYAYMMSETVYVCPECAHEWNPAELQESQGL
                     LVKDSNGNILHDGDAVIIIKNLPVKGSSSPIKAGTKVKNIRLVEGDHNIDCKIDGFGA
                     MALKSEFVRKA"
     gene            1374..1787
                     /locus_tag="P5243_06180"
     CDS             1374..1787
                     /locus_tag="P5243_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRS19070.1"
                     /translation="MKHVSKAYILSIVICMLSMYGYSQAPTISKKDEVFIRAEQMPEY
                     ETGKEQLGKDIADCVVYPKKAIELGISGTVVVKCVINTNGEACNPVVVKPVHELLDKA
                     AIDVIPCLGCFKPGVQGGVPVSVYFYIPIQFQIPK"
     gene            complement(1873..>2527)
                     /locus_tag="P5243_06185"
     CDS             complement(1873..>2527)
                     /locus_tag="P5243_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19071.1"
                     /translation="EAILPYDQYLHRFSAYFQQGNMESNGKYIDRNGKKVTYQTGPIL
                     WGEPGTNGQHAFYQLIHQGTKMIPCDFMAPAISHNPISDHHPKLLANYFAQTEALMMG
                     KTAAQVEAELKAAGKSAAEIAELLPYMVFEGNIPTNSLLFKKIDPRTLGSLIAMYEHK
                     IFVQGIIWNIYSFDQWGVQLGKQLANVILPELQNDVPVTSHDASTNGLINAYKAMRK"
BASE COUNT          834 a          391 c          492 g          810 t
ORIGIN      
        1 atgtttgtgt atctcgatgt acattttgca attttcacaa gttaattcac catgatggta
       61 cgtatattac tgaattagaa gaatattcac caaagataaa agaattagaa gcatacggtg
      121 gtaatcaatt gttgattcaa ggaggtatgc atcctcaatt aggtattgat ttttatgtgc
      181 aattgttttc agatttaaaa caacgtcatc ctaatattaa attacatgct ttgggaccac
      241 ccgaaatagc atacattgcc caaaaagagc atatgaccca caaagaagtg cttgaaaaac
      301 ttatagaatc aggtatggat agtttacccg gtgccggagc agaaattttg caagatcata
      361 tacgaaaaca attatcgccg ggtaagtgta atactcaaac atggttagat gtaatgcatc
      421 aggctcatat tcttggtata gttacttcgg ctactatgat gtatggacat attgaaacta
      481 ttgccgatag aatgcaacat cttgtgttgt tgcgtgagtt acaaaattct aaaccgattc
      541 attccaaagg ttttactgct tttattccat ggccgtttta tagtaaaaat actgttttgg
      601 ctcggcaaac cagaaaagaa tatgcaatac aacatttaga atatattcgt atgattgcca
      661 taagcagaat tatgttaacc aatatagata atattcaagc atcgtggctt acagtaggta
      721 ttcctactgc acaaatatgt ttgcatgccg gggctcatga tttgggaagt atcatgattg
      781 aagaaaatgt ggtttcgcaa gccggagcaa tgtttacggc caagaaagaa gagttgcaac
      841 aagcaattat tcaagccggg tttacaccta aattacgaaa tcaagggtat gatataattg
      901 aataaatcta tttactggat taagtttatg aatttgaaaa aaaagtttaa tgcttatata
      961 tatttaaaga ttacctttga ttgataaatt gctttgagaa taggaaaata tattaattac
     1021 aaaaactatg tcagaagaat taaaacaatg tccgcaatgt gcatctccct atgcctacat
     1081 gatgagcgaa acagtgtatg tgtgtcccga atgtgcacat gaatggaatc ctgcagaatt
     1141 gcaagaatcg caggggctac tcgtgaaaga ttcaaatgga aatattttgc atgatggcga
     1201 tgccgtgata ataatcaaaa atttaccggt aaaaggatca tcatcaccta ttaaagctgg
     1261 taccaaagtg aaaaatatcc gattggtaga aggcgaccac aacattgatt gcaaaattga
     1321 tggatttggc gctatggcat taaaatctga atttgtaaga aaagcataat actatgaaac
     1381 acgttagtaa agcatatatt ctaagtatag taatttgcat gctttcgatg tatgggtata
     1441 gtcaagcccc aacaataagt aagaaagatg aagtttttat tcgggcagaa caaatgcctg
     1501 agtatgaaac cggaaaagag caattaggta aagatatagc agattgtgta gtgtatccta
     1561 aaaaagctat agaattaggc atatcgggaa cagtagtagt aaaatgtgtt attaatacaa
     1621 atggtgaagc ttgtaatcct gtagtagtaa aacctgttca tgagttgtta gataaagcag
     1681 ctattgatgt tatcccttgt ttgggatgct ttaaaccggg tgtacaaggt ggtgtaccgg
     1741 tatctgtata tttttatatt cccatacaat ttcaaattcc aaaataaagg taatttagga
     1801 taagatatgt tatttaacaa aaaaagggta ttgattctgt caataccctt ttttgcatgt
     1861 atatcttaca ttttattttc gcattgcttt gtatgcattt atcaatccat tggtagatgc
     1921 atcgtgagaa gttaccggaa catcgttttg taattcaggt aaaattacat ttgccaattg
     1981 tttgcccaat tgtactcccc attggtcgaa actataaata ttccaaataa tgccttgtac
     2041 aaatattttg tgttcgtaca tcgctataag agagcccaat gtgcgagggt ctattttttt
     2101 gaataataga gaattggtag gaatattacc ttcaaatacc atatatggta ataattctgc
     2161 aatttctgca gccgattttc ctgcggcctt taattctgct tcaacttgag ctgcagtttt
     2221 tcccatcata agcgcttctg tttgtgcaaa ataattagca agtaattttg gatggtggtc
     2281 acttataggg ttatgagaaa tagccggagc cataaagtcg cacggaatca ttttggtacc
     2341 ttggtgaatt aactgataaa atgcatgttg accgttagtt cccggttcgc cccataaaat
     2401 cggacctgtt tggtaggtaa ctttttttcc gttgcggtca atatatttac cgttactttc
     2461 catattgcct tgttggaaat atgctgaaaa tctatgtaaa tattggtcgt atggaagaat
     2521 tgcttct
//
LOCUS       DAOTOW010000303         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32149_length_2525_cov_2.636032, whole genome shotgun sequence.
ACCESSION   DAOTOW010000303 DAOTOW010000000
VERSION     DAOTOW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32149_length_2525_cov_2.636032"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1300
                     /gene="pepD"
                     /locus_tag="P5243_06190"
                     /pseudo
     CDS             62..1300
                     /gene="pepD"
                     /locus_tag="P5243_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
     gene            1392..2273
                     /locus_tag="P5243_06195"
     CDS             1392..2273
                     /locus_tag="P5243_06195"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRS19072.1"
                     /translation="MKNILLVILIITCNNIFAQQDSIAPQQETPKQVNYTHVALVGTI
                     IPITIAGVYIYLNNVWWKEQRVPFHITDDLELRYALNLDKAGHFISSYFVSEIFSDVL
                     QVTGLPKKQSVWGGAALSIINSTIVEVKDAYSPYWGFSKFDMLANISGALLPVLQYHI
                     QAIKYLHFSWSYDFSHPSYYKSLPGHEDKSFIDDYERHNFWCTYDIWNALRLQNKESY
                     TPFFIQPAVGLSAQNLDGKGAGEHEWFIGMHINLMNLYRGSSKFIQYTCKYINFYHLP
                     APAIKIQPEITAYPITY"
     gene            2279..>2525
                     /locus_tag="P5243_06200"
     CDS             2279..>2525
                     /locus_tag="P5243_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRS19073.1"
                     /translation="MSLIVWNPTFETGIPEIDYEHKVFVSIIQRLESALQAKNKTQVI
                     NTIEELLKYADFHFTSEENIMIREEYPDYQWHKRIHER"
BASE COUNT          922 a          439 c          383 g          781 t
ORIGIN      
        1 agcccatgtt tttttgagga accatatcaa catgggcttg taaacaaact gtttacaagc
       61 ccatgttgat atggttcctc aaaaaaactc ccatatttca cacgattttg aaaaagatcc
      121 tattcaacca tatatttccg gtaatgtagt aaaagctcga aataccacat taggagcaga
      181 caacggcatt ggcttagcaa taattcttgc aatcttagaa ttacaaacta ttgaacatgg
      241 cacaattgaa gctctcttta cagtagatga agaaacaggt atgaccggag ctaaaaattt
      301 agatcatact tttttaacag ccaaatactt aataaatact gacaccgaac aaaaaaatga
      361 aataacaata ggttgtgccg gcggtgttga tgctaatttt gtttgggaca tacaatatga
      421 atcattaccg aacgattata ttacttgcac tatcagcatt aatggtttac aaggtggtca
      481 ttcaggattt gaaattcatt taggacgtgc aaatgcaaat attgttgcta ttgaaatact
      541 taatttacta caatctgaca taccaatttt actatcttct attaatggtg gtaatatgcg
      601 caatgctata ccccgtgaag catgtatagt aataggcata ccaaaagata aaaaacaagt
      661 atgcaaaaac cttctttcaa aatacagtac acaaataaaa aataaacatt ctgtaactga
      721 ccctcattta caaattgatt atacttttga agacaaaaca ctccaaataa ttccaaatac
      781 aattagtaaa tctattttac aagcattaac agaatgccct aacggtgtta tacaatttga
      841 agataaatca cgaggaattg ttcgtacttc tacaaattta tcaatcattt caacccacaa
      901 caataccgtg gaagtcaatt gcttgctacg aagtttcgat acaaaagaaa aatacgaatt
      961 agcaaaaaaa atggagaaga tattctcttc gcaacaagct tcgtgtgtat ttattggaga
     1021 ctatcccgga tggaaacctc aatggaattc cgaatttgtt caaatagttt caaacataag
     1081 caaacaagta tatggttcag aacctcatat acatgctgtg catgccgggt tagaatgcgg
     1141 catattttta ggaatttatc catatatgca ctgtatttca tttggcccca ccatacgtaa
     1201 ccctcattca cccgatgaag aagttgatat agattcggtt gcacaatttt ggaaagtatt
     1261 attgggtgta ttacaagcta ttccaactaa atcaatataa tacacaacac attaattctg
     1321 tgttattttt tttaaataac caattaaaac aaaactatta tgtacctttg caagtaaata
     1381 caattataga gatgaaaaat atactcttag ttattcttat tattacttgt aacaatatat
     1441 ttgctcaaca agactccata gcacctcaac aagaaacccc aaaacaagta aactatacac
     1501 atgtagcatt ggtaggaact attattccga taactattgc cggtgtatac atatatttaa
     1561 acaatgtatg gtggaaagaa caacgagtac catttcatat taccgatgat ttagaattgc
     1621 gctatgctct gaatttagac aaagcaggtc attttatttc atcatatttt gtttctgaaa
     1681 ttttttctga tgtactgcag gttacagggc ttccaaaaaa acaatcagtt tggggaggtg
     1741 ccgcattgtc aattattaat tctactatag tagaagttaa agatgcatat tctccatatt
     1801 ggggattcag taaatttgat atgcttgcaa atatctccgg agcattactc cctgttctgc
     1861 aatatcacat acaggcaatt aaatatcttc atttctcatg gagttacgac ttttcgcatc
     1921 catcatatta taaatcatta cccggacacg aagacaaatc ttttattgat gactacgaaa
     1981 gacacaattt ttggtgcaca tatgatattt ggaatgcatt aagattacaa aataaagaat
     2041 catatacacc attttttata caacctgcag taggattaag tgcccaaaat ttagacggta
     2101 aaggagctgg tgaacacgaa tggtttattg gtatgcatat aaatcttatg aatctatata
     2161 gaggttcttc aaaatttata caatacacat gcaaatacat aaatttttat catttgcctg
     2221 cccctgcaat aaaaatacaa cccgaaatta ctgcttatcc tattacttat tagaaattat
     2281 gagtcttata gtttggaatc caacatttga aaccggaata cccgaaattg attatgaaca
     2341 caaagtgttt gtatcaatta ttcaacgatt agaaagtgct ttacaagcta aaaataaaac
     2401 acaagtaata aatacaatag aagaattgtt gaaatatgca gattttcatt ttaccagcga
     2461 agaaaacata atgattcgtg aagaataccc tgattaccag tggcacaaac gaattcatga
     2521 acggt
//
LOCUS       DAOTOW010000304         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32164_length_2524_cov_2.851762, whole genome shotgun sequence.
ACCESSION   DAOTOW010000304 DAOTOW010000000
VERSION     DAOTOW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32164_length_2524_cov_2.851762"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="P5243_06205"
     CDS             <1..1328
                     /locus_tag="P5243_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRS19074.1"
                     /translation="KFILQKNGGAIALFTTTRVVYAFSNYTLNNNIYKYVFTRETNGK
                     PISIGKAFVQAKKITPNDFNQNKRSFTLFGDPALRLAIPEYNITIDSIQHIASSMFTD
                     TLTSNSVVHIQGSITNVEHLKVSSYNGTAYIKVFDKTQKITTLGNDGSNPFDFQVQNN
                     ILFQGKARITNGSFATEFIIPQDIYYFEGNGKISLYCTNDTIQGTGYLTVPINGTNDN
                     APFDNIGPTIRMYMGDTLFVSGNITNQTPTLLLYLFDSSGINISDAAIGHSITLILND
                     DFANPINLNEFYSASLDTYKYGTITYPFSTLPEGKHTLYIKVWDTRNNMSEKTIEFRV
                     VNSSNMQLYNLYTYPNPMKDIVTFHFQHNQELETIQVKISIFDNNGNCIHTITKTMEA
                     QGYTEQSIYWDGTTQNGSHISRGIYPYSVELISDDGKKVRESQKIIVIK"
     gene            1423..>2524
                     /gene="porV"
                     /locus_tag="P5243_06210"
     CDS             1423..>2524
                     /gene="porV"
                     /locus_tag="P5243_06210"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRS19075.1"
                     /translation="MNYKHISTLTLALILSIYSIQAQVGDKYHGITSQNLSKEYNVIT
                     TAVPFMTITPDSRAGGMGDVGVATSSDVWSQSWNASKYALSEKDMSAGISYTPWLENM
                     GIEDINLIYAAGYKKIKQTEAVGASLRYFSLGEIPAYEDNGNPADYTISPKEYAFDVS
                     YSRYLSDFFVMGMTGRFIYSNLTGDTDPDYKPGKSVAADISGTFFKDLANNSLLTLGF
                     NVSNLGSKIAYSSTQKDFIPTNLKIGAGYKMDIDNYNKIGFYLDINKLLVPTPPKKMY
                     ETNSAGNQVEVIFGNDNEDLSPVAGMFQSFVDAPGGFKEEMKEFIVSGGIEYWYNQQF
                     AIRAGYFHEAETKGDRKFFTAGAGLRLNVFGLD"
BASE COUNT          947 a          398 c          393 g          786 t
ORIGIN      
        1 aaaaatttat tttacaaaaa aatggcggtg ctatagcttt attcacgact acacgagttg
       61 tatatgcatt ttccaattat acactcaata ataatatata caaatatgta tttacaagag
      121 agacgaacgg taaacctatt tcaataggaa aagcttttgt acaagcaaaa aaaataacac
      181 caaatgattt taatcaaaat aaacgcagtt ttacgttatt tggagacccc gcattacgac
      241 ttgcaattcc ggaatacaat ataacaatag attctataca acatatagca tcatcaatgt
      301 ttaccgatac acttacatct aattctgttg ttcatattca aggttctatt accaatgttg
      361 aacatttaaa agtatcgtca tataatggaa cagcatatat aaaagtattt gacaaaactc
      421 aaaaaataac aactcttggt aacgatggca gtaatccatt tgattttcaa gttcaaaata
      481 atattttatt tcaaggcaag gctcgtatta caaatggttc atttgcaaca gaatttatta
      541 ttccacaaga tatttattat tttgaaggca acggaaaaat tagtttatat tgcactaatg
      601 atacaattca aggaaccgga tatcttacag ttcctataaa cggaacaaat gacaatgccc
      661 catttgacaa tataggcccc actattagaa tgtatatggg cgacacatta tttgtttcgg
      721 gtaatattac taaccaaacc cccacccttt tactctattt atttgatagt tccggaatca
      781 atatatccga tgctgctata ggacatagta ttacccttat tttaaatgat gactttgcta
      841 atcctattaa tttaaatgaa ttctattctg cttcgcttga tacatataaa tatggtacta
      901 ttacatatcc tttttctaca ttacccgaag gaaaacatac cttatatata aaagtttggg
      961 atactcgcaa taatatgagt gaaaaaacaa ttgaatttag agttgtaaat agttccaata
     1021 tgcaattata caatctgtat acctatccaa atccaatgaa agatattgtg acatttcatt
     1081 ttcagcataa tcaagaatta gaaaccattc aggtaaaaat ttctatattt gacaataatg
     1141 gtaattgcat tcatacaata actaaaacaa tggaagctca gggatataca gaacaatcta
     1201 tatattggga cggaaccaca caaaacgggt ctcacatatc acgaggcatt tacccatata
     1261 gtgtagagtt aataagcgat gacggcaaaa aagtacgtga aagtcaaaaa ataatagtaa
     1321 taaaataaca gagcatatat actaaaacta atgaatttga gttacaatga aaaaaattat
     1381 atatttgcat ttattaaaca ttgaatttaa caaattaaac tcatgaatta caaacatatt
     1441 tcaacactaa ctttagcatt aattttatcg atttattcaa tacaagcaca agtaggcgac
     1501 aaatatcacg gaattacaag ccaaaatctt tcgaaagaat ataatgtaat aactacagca
     1561 gttcctttta tgactattac tcccgattca cgtgcaggag gaatgggcga tgtaggggta
     1621 gctacaagtt ctgatgtatg gtcgcaaagt tggaatgctt caaagtatgc cttgtcagaa
     1681 aaagatatgt cagcaggaat ttcatataca ccatggttag aaaatatggg tatagaagat
     1741 ataaacttaa tatatgccgc aggatacaaa aaaataaaac aaactgaagc cgtaggagct
     1801 tcgttacgtt atttttcatt aggtgaaata cctgcatatg aagataatgg taatcctgct
     1861 gattatacaa tttcacctaa agaatatgct tttgatgtat catattcgcg gtatttatca
     1921 gatttctttg taatgggtat gacgggtaga tttatatatt caaaccttac cggcgatacc
     1981 gaccctgatt ataaacccgg gaaatctgtt gctgcagata tttcgggaac gttcttcaaa
     2041 gatctagcaa acaatagttt acttacatta ggttttaatg tgtcaaattt aggttcaaaa
     2101 atagcctatt cttcaacaca aaaagatttt atacctacaa acttaaaaat tggtgcaggt
     2161 tacaaaatgg atattgacaa ttataataaa attggtttct atttagacat aaataaatta
     2221 cttgtaccaa caccacctaa aaaaatgtat gaaacgaata gtgcaggcaa tcaagttgaa
     2281 gttatttttg gtaatgacaa tgaagacctt tcgcctgttg caggaatgtt tcaatcattt
     2341 gtagacgcac ccggaggatt caaagaagag atgaaagaat ttattgtttc tggtggaata
     2401 gaatactggt ataatcaaca atttgcaata cgtgccggat attttcatga agcagaaaca
     2461 aaaggtgaca gaaaattctt tactgctgga gcaggattac gtttaaatgt atttggatta
     2521 gatt
//
LOCUS       DAOTOW010000305         2522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32201_length_2522_cov_3.444264, whole genome shotgun sequence.
ACCESSION   DAOTOW010000305 DAOTOW010000000
VERSION     DAOTOW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32201_length_2522_cov_3.444264"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..549
                     /locus_tag="P5243_06215"
     CDS             34..549
                     /locus_tag="P5243_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19076.1"
                     /translation="MNLNIFGLRNADRVVNLFNDIIGIAYTDADGREQCKFWHGSTKP
                     GLFYLGDKLMNGKGTFILMPGFYKACWQLGLHKQQYRALVQRGTGIFKGWRDANKNGI
                     LDYTGKIWTDVAGLNLHTTSYKTNQEKVGAWSAGCQIIQNAADFEECMSIVEESAKLY
                     GSVFSYKLFQL"
     gene            867..1514
                     /locus_tag="P5243_06220"
     CDS             867..1514
                     /locus_tag="P5243_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19077.1"
                     /translation="MGKHSNRTQLSARFTDKIREKFNEISEVNPEYTDGNIIEAIIDQ
                     TIGEGINVIAAQEKIEYLQTINDNLTQQIDAQQQEIDALIAQANTAKDGELESEQLQA
                     ELFKKNGMIADLEAKLQEMTNIANENARLLQAHTLEKENPKAGTYVVELPPFADEIMQ
                     ITCNRLREKLQVPVSPNTLLTDLFVKYTVEQPADFAFPTVLNKTEIKVLISKYNG"
     gene            1507..1800
                     /locus_tag="P5243_06225"
     CDS             1507..1800
                     /locus_tag="P5243_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19078.1"
                     /translation="MGNLIDEFKQNAAKRLVKQFLPMIEENLSQIDPSIDELLSKTEV
                     TEEQYATVIIWKHSSGIYNASIAILNKEDNSIVKLTDTKPVKDVILSIINPLK"
     gene            1806..2378
                     /locus_tag="P5243_06230"
     CDS             1806..2378
                     /locus_tag="P5243_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19079.1"
                     /translation="MPLDDLLEKEPENEVLHEQEQTQSSYSFSTGSLDDYKSLIGEIK
                     QPKKEYPPQQEEEIEEIATDKPTIAKVSRVAKTKASQFLVSGGDKAIAFLCDMISDND
                     GEENFNADDEEKRELQEYVAMLLPDDKEVIPPWLMLVVTAGLIYGPKFKDAFKLKKYK
                     KALEEQQIKNAQIQQENELLRQQLQQKTNA"
BASE COUNT          965 a          413 c          497 g          647 t
ORIGIN      
        1 aacccccagg gtatcaccag tgtctacaat aatatgaacc tcaatatatt tggtttacgc
       61 aatgccgata gggttgtaaa tctatttaac gacattattg gaatagccta taccgatgct
      121 gatgggcgag aacaatgcaa attttggcac ggaagtacaa agcccggatt gttttatttg
      181 ggcgataaac ttatgaacgg caaaggcaca tttatactta tgccggggtt ttataaggca
      241 tgttggcaac taggattaca caaacaacag tatagagcat tggtgcaacg tggtaccgga
      301 atatttaagg ggtggcgtga tgcgaataaa aacggtatac tcgattatac cggtaaaata
      361 tggactgatg ttgcagggct taatttacat acaacttcat acaaaaccaa tcaagaaaaa
      421 gtaggtgcct ggagtgccgg ctgccaaata atacaaaatg ctgcagattt tgaagaatgt
      481 atgagtattg tagaagaatc ggcaaagctc tatggtagtg tgttttcata taaattattt
      541 cagttatgac aattgtacta ttcatagcag gtattctctg tattcgatgt ttgtacatat
      601 acattgctac ataccatata aataaagaag aatgaagttt ttacaacgaa tattcgagcg
      661 tgtacgcatc gaaaataaaa aacacaaaga tgaatacgaa cggttatata aaactggtag
      721 aaatgaagcc tgtatgtgtg gtagtggcaa aaagtttaaa cagtgctgca tacataaaaa
      781 acgcaatttc aagcctcgta gattatttcc gaatttgttt tggagtgaaa ttataaatta
      841 aaataaataa ttaatatagt aaaaacatgg gaaaacacag taacagaacg caattatcgg
      901 cacggtttac cgataaaatt cgcgaaaaat tcaacgaaat tagcgaggta aatcccgaat
      961 ataccgatgg taatattata gaagctataa tagaccaaac cataggagag gggataaatg
     1021 taatagctgc acaagaaaaa atagaatatt tgcagactat taacgataat ctaacgcagc
     1081 aaattgatgc tcaacagcaa gaaattgatg cactgattgc acaagcaaat acggcaaaag
     1141 atggcgaatt ggaaagtgag caattgcaag cagaattatt caaaaaaaat ggaatgattg
     1201 cagatttaga agcaaaactg caagaaatga caaatattgc caatgaaaat gcacgattgc
     1261 tacaagcaca cacgctcgaa aaagaaaatc caaaagccgg tacctatgtg gtagaacttc
     1321 caccatttgc cgatgaaatt atgcaaatta catgtaatcg tttgcgagaa aaattacaag
     1381 ttcctgtatc tcctaatacc ttacttaccg atttgtttgt aaaatatacg gtagagcaac
     1441 cggctgactt tgcatttcca acagtactaa ataaaacaga aattaaagtt ttaataagca
     1501 aatacaatgg gtaatttaat agacgaattc aaacagaatg cagctaaacg gcttgtaaaa
     1561 cagtttttgc caatgataga agaaaatcta tcacaaatag atccttcgat tgacgaactt
     1621 ttgtcaaaaa cagaagtaac cgaagagcaa tatgcaaccg tgattatatg gaagcatagt
     1681 agcggtatat acaatgcgag tattgctata ttaaataaag aagacaatag cattgtaaaa
     1741 cttaccgaca ctaaaccggt aaaagatgta attctatcca ttattaaccc tctaaaataa
     1801 cgtgtatgcc actcgatgat ttattagaaa aggaacccga aaacgaagtg ctacatgaac
     1861 aggagcaaac gcaaagctcg tattcattta gtaccggatc tctcgatgac tataaaagcc
     1921 ttataggaga gataaaacag cctaaaaagg aatatccacc acaacaggag gaggaaatag
     1981 aggaaattgc aactgataaa cctactattg caaaagtaag tagagttgca aaaaccaaag
     2041 catcacaatt tcttgtaagt ggaggcgata aggctattgc ttttttgtgc gatatgattt
     2101 cagacaacga tggcgaagaa aattttaatg ccgatgacga ggaaaaacgc gagctgcaag
     2161 aatatgttgc aatgctattg cctgatgata aagaagtaat accgccgtgg cttatgttgg
     2221 ttgttactgc agggcttata tatggtccta aatttaaaga tgcttttaaa ctcaaaaagt
     2281 ataaaaaagc actggaggaa caacaaatta aaaatgctca aattcagcaa gaaaacgaac
     2341 tattacgcca acaacttcaa caaaaaacaa atgcataatt ttgcagctcc aaagccggga
     2401 cgtgatccaa aatgtatact catacttggt accaatggta cgggtaagag tacactagct
     2461 tgcaatatga tgaacgatgc aggcaaagga cttatggtat taccacagtt tgatgattgg
     2521 gc
//
LOCUS       DAOTOW010000306         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32254_length_2519_cov_3.017451, whole genome shotgun sequence.
ACCESSION   DAOTOW010000306 DAOTOW010000000
VERSION     DAOTOW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32254_length_2519_cov_3.017451"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="P5243_06235"
     CDS             complement(<1..1616)
                     /locus_tag="P5243_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19080.1"
                     /translation="MKTSMLRFFVCIVSISLFINTYGQQDSISTELNKTIIVPSDYKP
                     QISDAIRLSVPPRAYDTLDLRVDLYYSIQSKPLETAYTLKTLKPVSVVGDKLQELYKG
                     YAAVGFGNYSQSYVNAKYTTERSRLKQQGIEIYHNASAGKVRFENDAKLPAGYTQDYI
                     YAYAKRFNPSVITSASIKPMYSSAMRYGYQMYDTLFPMVPDTILTKRSIKRNFFTNEI
                     SLGVQSATSDKSKLRYTSNLNHILTIVNPKTVENALFFEANAIKQFEQIQTGALLDVS
                     WSGLNFAPIDTLVSKNSARLQLLPFVGTTVDDWSLRVGVNLIESIGLNTFKAYPDVRF
                     DYNLLSYSLIPYIRYSGNLQHYSMNTMLFENPYTSNYVYLRPTNYKMNIEFGAKGRLL
                     RTIPFVAACTISQFENMYFWVNDVVGTNGIQNSFIPVYDNGTRITLHGETGIKRKNLD
                     VQAVVDYHQYALDSIQRAWHRPSIESKLLFKYAIVNPITNTNKMIVSSQFFYNDLLYA
                     QTVDGKVHKMNSIFDVNLGIEYYYTSVLVF"
     gene            complement(1623..>2519)
                     /locus_tag="P5243_06240"
     CDS             complement(1623..>2519)
                     /locus_tag="P5243_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19081.1"
                     /translation="SLSFEVAQELYLDGDCDKAQPLLSAYIQKYGEGFFLLHAHFYRA
                     ECFFSKGYEFEALPDYEYVLQYTKNSFTETSLVNASQIAINSYQYDKALGFLQRLENE
                     AELKPNLLVARKGMMVCYEKLKNNSKIIEIGDKYIVTEKITPADKRWANMLMAKAYEA
                     MQDSVNALVKYKTVAVEHATKEGAEARYYVALYAFNQQKYDEAEKEIIDFLEKNSPHQ
                     YWLGKSYILWAKIFIQRNELFQARYTLQNVMQHYKNKEDGIIAEANDMLNLITDIEIG
                     QKQKAQQEVTIPMGNNPELFKK"
BASE COUNT          848 a          429 c          348 g          894 t
ORIGIN      
        1 aaaaaaacca aaacacttgt ataataatat tctattccaa gatttacatc aaaaatagaa
       61 ttcatcttat gtactttacc gtcaacagtt tgtgcataga gtaaatcatt ataaaagaat
      121 tgagaactta caatcatttt atttgtatta gtaataggat ttacaatggc atatttaaac
      181 aataatttac tttcaataga gggtctatgc catgctcgtt gaatactatc aagagcatac
      241 tgatgataat caacaaccgc ctgtacatca agattttttc ttttaatacc ggtttcgccg
      301 tgcagagtta ttcgagtacc attatcataa acaggtataa aactattctg tataccatta
      361 gttcctacaa catcattaac ccaaaaatac atattttcga actgactaat agtacatgca
      421 gccacaaatg gtattgtacg aagcaaacgc ccttttgctc caaattctat attcatttta
      481 taatttgtag gacgtaaata cacataatta cttgtataag gattttcaaa caacatagta
      541 ttcatagaat agtgctgtaa attcccggaa taacgaatat atggaatcaa tgaataactc
      601 aataaattat aatcaaaacg tacatcagga tatgctttaa atgtatttaa accaatagat
      661 tctattaagt ttacacctac tcgcaaagac caatcatcaa cagtagtacc tacaaaaggc
      721 aacaattgca aacgagctga attttttgac acaagcgtat caattggtgc aaaatttagt
      781 ccactccacg acacatcaag caaagcaccg gtttgaattt gttcaaattg cttaattgca
      841 ttagcttcaa aaaatagagc attttcaact gttttgggat ttactatagt taaaatatga
      901 ttgagattag aagtatatct caattttgat ttatcagatg tagcagattg tacccctaaa
      961 gatatctcat ttgtgaaaaa atttcgttta atacttcgtt tagttaaaat agtatcaggc
     1021 accattggaa agagagtgtc atacatttga tacccgtaac gcatagcact gctatacatt
     1081 ggttttatag aggccgaagt tataacagaa ggattaaacc gttttgcata tgcatatata
     1141 taatcctgag tatatcctgc cggtaatttt gcatcatttt caaatctaac tttacctgca
     1201 gatgcattat gatatatttc tattccttgt tgctttaaac gagaacgttc tgtagtatat
     1261 ttagcattaa cataactttg agaataatta ccaaagccta ccgcagcata tcctttatac
     1321 agttcttgta atttgtcgcc aaccacactt acaggtttta atgtttttaa tgtatatgca
     1381 gtttctaaag gttttgattg tatagaataa tataaatcta ctcgtaaatc taaagtatca
     1441 tatgctcgtg gtggcactga caatcgtata gcatcagaaa tttgaggttt ataatcagat
     1501 ggcactatta tagttttatt taattctgtt gaaattgaat cttgctgtcc ataggtattt
     1561 ataaacaaac taatacttac aatacaaaca aaaaaacgta acatacttgt tttcatatat
     1621 aattattttt taaataattc aggattatta cccattggaa ttgtaacttc ttgttgagct
     1681 ttttgttttt gccctatttc tatatcggta attaaattaa gcatatcatt agcttcggct
     1741 attataccat cttctttgtt tttataatgt tgcattacat tttgtaatgt ataccgagct
     1801 tgaaacaatt catttcgttg aataaatatt ttagcccata ggatatacga tttaccaagc
     1861 caatattgat gtggcgagtt tttctccaga aaatcaatta tctctttttc tgcttcatca
     1921 tacttttgtt gattaaatgc atataacgct acataatatc gtgcttcggc accttctttt
     1981 gtagcatgct ccacagcaac tgttttatat tttactaatg catttacaga atcttgcatt
     2041 gcctcatatg ctttagccat aagcatatta gcccatcgtt tatctgcagg agtaattttt
     2101 tcggttacta tatatttgtc gccaatttca ataatcttag aattattttt aagtttttca
     2161 tagcatacca tcattccttt tcgtgcaact aataaatttg gttttaattc tgcttcattt
     2221 tctaatcgct gcaaaaatcc taatgcttta tcatattgat acgaatttat agctatttgt
     2281 gaagcgttaa ctaatgaggt ttcggtaaat gaatttttag tatactgcaa tacatattca
     2341 taatcaggca aagcctcaaa ttcatagcct tttgagaaaa aacattctgc acgataaaaa
     2401 tgagcatgca ataaaaagaa tccctcacca tatttctgta tatatgcact taataatggt
     2461 tgagctttat cacaatcgcc atcaagatac aactcttgag caacttcaaa actgagtga
//
LOCUS       DAOTOW010000307         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32384_length_2512_cov_3.293040, whole genome shotgun sequence.
ACCESSION   DAOTOW010000307 DAOTOW010000000
VERSION     DAOTOW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32384_length_2512_cov_3.293040"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="P5243_06245"
     CDS             complement(<1..1123)
                     /locus_tag="P5243_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS19082.1"
                     /translation="MKKTFLTNLALLVFLNLLVKPFWIFGIDRTVQNTVGAHEYGLYA
                     AMFSLSIMLNIILDLGITNFNNRNIAQHSQLLRKYFSNIVILKCLLGVVYAIIAIIVG
                     ALLNFTLERYYLFIFLIFNQFLLSFILYLRSNISGLHKYKTDSLVSVIDRVLMIILCG
                     IVLITKQHEFKIQWFVYAQTISYSITCAIAFIIVYTNTSLFRIKFDFTFLLVILKQSY
                     PFALLVLLMGLYTRMDMVMVEYMLPNGAEQAGIYAQSYRILDAVSMFAYLFATLLLPM
                     FSKMLRFGESVAQLTKLSFILLIIPTLIFVICTWFYGWELMKLMYHDHYDESAEIYKV
                     LIIGFIPISSSYIFGTLLTANNSLKYLNIIAASGMVLNFI"
     gene            1298..2344
                     /locus_tag="P5243_06250"
     CDS             1298..2344
                     /locus_tag="P5243_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19083.1"
                     /translation="MIEQSQYHSIISKAWHEYNPKREIASIVDVSANVSTNRVFAVTF
                     TDGSFIMAKVSNFGKFENFKEDHTIINVMANNLEYPYDLFLSSSLMKNDELFLYKYDD
                     SSIDVWLVFYRVVRIKNKLPKRLYEEQIRILGRELAKFHKICDTMTPVLPQSSKNLQK
                     DIHSLLRRLQKEGSDQKYEGNVDLIRKHCDIFLEQYDKLGMDTFSKIPVFVDWNIGNF
                     SVRDNGTFFSRWDYDWFRMSSRILDFYSFSRVVSDIGDKTDFSYTSSQLNEDRFLLFL
                     KEYHAVFPLTEAEIRFMKEAYRFFILNYVINNGKYFFAPSYAKKLQHEAYEMYLPQLD
                     SVCSCDRILYELGF"
BASE COUNT          917 a          327 c          399 g          869 t
ORIGIN      
        1 atataaaatt caaaaccata cctgaggctg ctataatatt gagatatttt aagctattgt
       61 tagctgtaag taatgttcca aaaatatacg aagatgatat tggaataaat ccaattatta
      121 aaactttata tatttctgct gattcatcat agtggtcatg atacataagt ttcattaatt
      181 cccacccata gaaccacgtg caaattacaa agatgagagt tggaattatg agtaaaatga
      241 atgaaagttt ggtaagttgg gcaactgatt cgccaaatcg cagcattttt gaaaacatag
      301 gtagcaataa tgttgcaaat aaatatgcaa acatacttac agcatctaat atacgatatg
      361 attgtgcata aatgcctgct tgttctgctc cgtttggcaa catatattct accataacca
      421 tatccattcg tgtataaaga cccataagta aaactaacag tgcaaaagga taactttgtt
      481 tgagtattac taataaaaat gtaaaatcaa atttgattct aaacaatgat gtgttggtat
      541 atactataat aaaggcaata gcacacgtaa tactgtagga gatagtttgt gcatatacaa
      601 accattgtat tttaaattca tgttgttttg ttatcaatac aatgccacat aatataatca
      661 taagtacgcg gtctattacg gaaaccaaac tatctgtttt gtatttatgt aatcccgaaa
      721 tattggaacg taaataaaga ataaacgata ataaaaattg attgaaaata agaaatataa
      781 agagataata tctttcaagg gtaaaattga gcaatgctcc tactataatg gcaataattg
      841 catataccac acccaacaaa cattttaata taacaatatt tgaaaagtat tttcgcaata
      901 attggctatg ttgagctata tttcggttgt tgaaatttgt aattcctaaa tcgagaatta
      961 tattgagcat gattgataaa ctaaacatgg ctgcatataa accatattca tgagctccta
     1021 cagtattttg aacagttctg tcaatgccaa atatccaaaa gggtttaacc aataaattga
     1081 gaaaaaccaa taaggctaaa tttgttaaaa atgttttttt cacattgaag aatatttacc
     1141 ggcaaatata atgaagctta ttgtataatt taaaacaatt tgtggtaaat aaaaaactaa
     1201 cattatatat tgggctttgc aagaatttat gtatatttga attactatga ttacgtgtgc
     1261 ggtacaagta taaattttga taaatcaaca aaaatacatg atagaacaat ctcaatatca
     1321 ttctattatt tcaaaagcat ggcacgaata taatcctaaa cgagaaattg catcaatagt
     1381 ggatgttagt gccaatgtat ctacaaatag agtatttgct gttacattta ccgatggtag
     1441 ttttattatg gcaaaagttt cgaattttgg taagtttgaa aattttaaag aagatcatac
     1501 tatcattaat gtaatggcca ataatttaga atatccgtat gatttatttc tttcaagttc
     1561 attgatgaag aatgatgaac tttttttata taaatatgat gattcttcta ttgatgtatg
     1621 gttagttttt tatagagttg tacgaattaa aaataaatta ccaaaacgac tatacgaaga
     1681 acaaatacgc atactgggta gggagttggc aaaattccat aaaatatgcg ataccatgac
     1741 accggttttg cctcagtcat caaaaaattt acaaaaagac atacattctt tgctgagacg
     1801 attacagaaa gaagggagcg accaaaaata tgaaggaaat gttgatttaa tccgtaagca
     1861 ttgtgatata tttttggaac agtatgataa gttgggaatg gatacatttt ctaaaattcc
     1921 tgtttttgtt gattggaata ttggtaattt ttcggttcgt gacaatggta cttttttttc
     1981 gcgttgggat tatgattggt ttagaatgag ttctcgcatt ctcgattttt attcgtttag
     2041 cagggttgta tccgatatag gtgataaaac agattttagt tatacatcat cacaactcaa
     2101 tgaagacaga tttctattgt ttcttaaaga ataccatgct gtatttccgc ttactgaagc
     2161 cgaaatacgt tttatgaagg aagcatatcg ttttttcatt cttaattatg ttataaacaa
     2221 tggtaaatat ttttttgctc cctcatatgc aaaaaaatta cagcatgaag cttatgaaat
     2281 gtatcttcca caacttgatt ctgtatgtag ttgtgataga attctctatg aattagggtt
     2341 ttaatagttg tttttttaca attcttttga tgataaaact caatatgtaa aagtgtttgg
     2401 gaaaggttga ataaataaaa aaatatgtat ttttacaaga atacatagtt tgaatacata
     2461 taatactccc tattaatagg acagtttagt tatctttttt agatataatt tt
//
LOCUS       DAOTOW010000308         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32399_length_2511_cov_3.565961, whole genome shotgun sequence.
ACCESSION   DAOTOW010000308 DAOTOW010000000
VERSION     DAOTOW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32399_length_2511_cov_3.565961"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="P5243_06255"
     CDS             complement(<1..236)
                     /locus_tag="P5243_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HRS19084.1"
                     /translation="MTDLELYKKELLAQGAEKIFTKTKNLTDTPFSYCGGCGHGVAHR
                     LIMEAIEELDITHQTIGVAPVGCSVIAYDFMNIDV"
     gene            complement(243..1313)
                     /gene="vorB"
                     /locus_tag="P5243_06260"
     CDS             complement(243..1313)
                     /gene="vorB"
                     /locus_tag="P5243_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRS19085.1"
                     /translation="MGAEIQLMKGNEAIAEAAIRAGADAYFGYPITPQSEIIEYLAHE
                     DPYNKRTGMVVLQAESEIAAINMVYGAAACGKKAMTSSSSPGISLKQEGISYMVGAEL
                     PGLIVNVARGGPGLGTIQPGQADYFQTVKGGGHGDYNMIVLAPSTVQEQYDHVGLGFD
                     LAFKYRNPVMILSDGAIGQMMEKVQLSPQKPREQKIYDWATTGKTPDRERNYITSLFL
                     EAEIQERKNIQLQEKFKRMKESEVRYEEIMTKDADYILVAYGISARISESVIDIARKN
                     GIKLGLLRPITLWPFPSQRIFELAERIKGILCVEMSAGQMIEDVQLAVQGKIEVKHYG
                     RMGGSIASPSEVYNNFISLFLK"
     gene            complement(1337..1558)
                     /locus_tag="P5243_06265"
     CDS             complement(1337..1558)
                     /locus_tag="P5243_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS19086.1"
                     /translation="MAKARGFVEFNTEACKGCELCVKACPFDVLDMNQKVNGKGYHYS
                     YMKNYDKCTGCASCAMVCPDVVITVYKVK"
     gene            complement(1680..>2511)
                     /locus_tag="P5243_06270"
     CDS             complement(1680..>2511)
                     /locus_tag="P5243_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19087.1"
                     /translation="NILINFGTPIPVKQHEDLYKKNPQEAQNKMRDDIETELQKQMIH
                     IKHTDENYEIFDSLRYICEKITQNYYQFKGKKAKKQFDTHKKIIELLDICYTNKPHVY
                     EEIKNATSSYTNEIKKYNFKDWLVTTKGGDTFSIVLNILILIALSPIFCIGYIFHGYL
                     FKLFNSLAKKLAKDVQFHNSLNFCFTYLVGPFIYALYSIIFILVTNFTWWYIFPFCIG
                     LFISGIISFEYYIAAKKTYHMIRYNFIQLTTPQITDSIEKQRRKIIKMFSTLLHEQNQ
                     "
BASE COUNT          751 a          432 c          350 g          978 t
ORIGIN      
        1 acatcaatat tcataaagtc atatgctatt accgaacagc caacaggagc aactcctatt
       61 gtttgatgtg ttatgtcaag ttcttctatt gcttccatta ttaaacgatg agcaacgcca
      121 tgcccacacc ctccacaata tgaaaatgga gtatctgtaa gatttttagt tttagtaaag
      181 attttttcag caccttgtgc taataattct tttttatata gttccaaatc agtcataatc
      241 tattatttca aaaataacga aataaaatta ttgtaaactt cgcttggtga agctatagaa
      301 ccacccatgc ggccataatg ttttacttca atttttcctt gaacggcaag ttgaacatct
      361 tctatcattt gtcctgcgct catttcaaca cacagtatac ctttaattct ttctgctaat
      421 tcaaatatac gttgcgaagg gaatggccat aacgtaatgg gacgtaggag tcctaatttt
      481 ataccatttt tgcgagcaat atcaattacc gattcagaaa tacgagcact aataccatat
      541 gcaacaagta tataatcggc atcttttgtc attatttctt cataacgaac ttcagattct
      601 ttcattcgtt tgaatttttc ttgtaattgt atattttttc gttcttggat ttcggcttct
      661 aaaaataatg atgttatata attacgttct ctatctggtg tttttccggt agtagcccaa
      721 tcatatattt tttgttctct tggtttttgg ggagaaagtt gtactttttc catcatttga
      781 ccgattgctc cgtcgcttaa aatcattacc ggatttctgt atttgaatgc taaatcaaat
      841 cctaatccta catggtcgta ttgttcttga acagtacttg gagctaatac aatcatattg
      901 taatcaccat gtccgccgcc ttttactgtc tgaaaataat cagcttgtcc gggttgaatt
      961 gtacctaatc ctggtcctcc acgtgctaca tttactatga gtccgggtaa ttcggcacct
     1021 accatatacg aaattccttc ttgttttaag cttattccgg gacttgatga agatgtcatt
     1081 gctttttttc cacacgcagc tgctccgtaa accatattta tggcagctat ttcgctttcg
     1141 gcttgtaata ctaccatacc ggtgcgttta ttatatggat cttcatgtgc taaatattca
     1201 ataatttctg attgtggtgt aattggatat ccgaaataag catctgctcc tgcacgaata
     1261 gccgcttcgg ctatagcttc gtttcctttc attaattgta tttctgcccc catatatatc
     1321 aaatcaaaat gttgaattat tttactttat acacagttat tacaacatca ggacatacca
     1381 ttgcacaact tgcacaaccg gtacatttat cataattttt catataactg taatgatatc
     1441 ctttgccatt tactttttga ttcatgtcca atacatcaaa cggacaagct ttaacacata
     1501 attcgcatcc tttacatgct tctgtattaa attctacaaa ccccctcgct tttgccattc
     1561 aattatttat tattgtttgt ttacaatatt ttaacgtacg caaatttata cgttttcagc
     1621 aattaaaaaa attgtatctg ttctatttgg acttatttta atgctaaacc tttcaaattc
     1681 tactgatttt gctcatgtaa tagtgttgaa aacattttaa taattttcct gcgttgtttt
     1741 tctatactat ctgtaatttg tggtgtggta agttgtataa aattatatcg tatcatatga
     1801 tacgtttttt ttgctgcaat ataatattca aaactaataa ttccgctaat aaataaacct
     1861 atgcagaatg gaaatatata ccaccatgtg aaatttgtga caagtataaa tataatgcta
     1921 tatagagcgt aaataaaagg tcctaccaaa taggtaaaac aaaaatttaa actattatga
     1981 aattgtacat ctttagcaag tttttttgct aatgaattaa ataatttgaa taaatatccg
     2041 tgaaaaatat atccaataca aaatattggt gataatgcta taagaataag tatgtttaga
     2101 acgatactaa aagtatcacc acctttagtt gtaactaacc agtctttaaa attgtatttt
     2161 tttatttcat ttgtatatga acttgttgca tttttaattt cttcatatac gtgcggttta
     2221 ttagtataac atatatctaa taattcaata atttttttgt gagtatcaaa ttgttttttt
     2281 gcttttttcc ctttgaattg ataataattt tgtgttattt tttcacatat atatcttaaa
     2341 gaatcaaata tttcataatt ttcatcggta tgttttatgt gaatcatttg tttttgtaat
     2401 tcagtttcta tatcatcacg cattttgttt tgagcttctt gcggattttt tttgtataaa
     2461 tcttcatgtt gttttacggg aataggtgtg ccgaaattaa taagaatatt a
//
LOCUS       DAOTOW010000309         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32538_length_2504_cov_2.931401, whole genome shotgun sequence.
ACCESSION   DAOTOW010000309 DAOTOW010000000
VERSION     DAOTOW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32538_length_2504_cov_2.931401"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..320)
                     /locus_tag="P5243_06275"
     CDS             complement(30..320)
                     /locus_tag="P5243_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19088.1"
                     /translation="MNHSFLGILFYRIEPRISLIYKLQHSEFSIHYNILSQAMQILSN
                     NAYAYTTLETWIPMSPNIKPLVSNNYTCSYTLNYNGFSGMVEGYLKKITVVR"
     gene            complement(280..>1044)
                     /locus_tag="P5243_06280"
     CDS             complement(280..>1044)
                     /locus_tag="P5243_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19089.1"
                     /translation="KMKEGNMNANTGQFSFSPLTIDANIEGPIKKDTASYFFAIRKSV
                     LDIFMNWSKQGFEVPLFYDINTKINYKINSKNRLFLSVYNGSDIFINENSKNTSNNIT
                     STIRWLRIINPKLIVNTSYIYSKYGNNTIFQIDEKPTNWKTGITDNSIKSKISWFPNT
                     NNTFSFGVNIQYHNCIPGNSGNPQTSISDMNLFENSIFAMHKIKINNSFSAHYGVRIQ
                     SYMNTGKGVWYSLNENYEPIKPNYESQFSWNSFLQN"
     assembly_gap    1045..1054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1055..1704)
                     /locus_tag="P5243_06285"
     CDS             complement(<1055..1704)
                     /locus_tag="P5243_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS19090.1"
                     /translation="MKNYIILFFLIYSSLGFCQSSYTISGFVRDTVTGEELIGVSIYD
                     YQTNKTTLTNENGFYSILLEEGVHILEFSHVGYSITIDTITIFKHIHHNVFLQKSSIE
                     LNTIIVSATKYELSGLVKKMPLQQIEQTPTIFGVPDVIKVLQLQPGIKNIGDGASGMY
                     VRGGNRDQNMIIIDDAPIYNISHMYGFVSAINPTMLKDATFYNSNIPAEFGGKISSV"
     gene            complement(1759..>2504)
                     /locus_tag="P5243_06290"
     CDS             complement(1759..>2504)
                     /locus_tag="P5243_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19091.1"
                     /translation="LNYENQIKSNQIKSIIFSKLFHIISASLLSVFLYSCKKDENIQT
                     KQQYSKELIVVDSVNKNAVKLKISSDDKLYLDEWSENDFMLIANNKGIEEYESQKPII
                     ENNEICQESNFNHFDSSKTLLIEILSNDFKFPTSKYVLKKIKSKAFLKCDFNLLPYHH
                     DIWQHRNALAVKIRNESGALLCLNNAWVRVYDNGNYLDVEPYQQFEIKNKYDMVWSIR
                     PSNTLGWIVVRVTYNNSNKGEVSCLFTIE"
BASE COUNT          796 a          359 c          339 g         1000 t
ORIGIN      
        1 aagactaaca tcttccttct cttaattttt tatcggacaa cagtgatttt ttttaaatat
       61 ccctctacca ttcccgaaaa tccgttataa ttcaatgtat atgaacacgt ataattattg
      121 cttacaagag gttttatatt cggtgacata ggaatccaag tttctaacgt tgtatatgca
      181 tatgcattgt tagatagtat ttgcatagct tgcgataata tattgtaatg tattgaaaat
      241 tctgaatgtt gtaatttgta tataagtgat atacgtggtt caattctgta aaaaagaatt
      301 ccaagaaaac tgtgattcat aatttggttt tataggttca taattttcat ttaaactata
      361 ccatacacct ttacctgtat tcatatatga ttgaattcga accccataat gtgccgaaaa
      421 agagttatta atttttattt tatgcattgc aaatatgcta ttttcaaaca aattcatatc
      481 tgaaatactt gtttgcgggt ttcctgaatt cccgggaatg caattgtgat attgtatatt
      541 aacaccaaat gaaaatgtat tgtttgtgtt agggaaccaa gagatttttg actttattga
      601 attatctgta ataccggttt tccaatttgt tggtttttca tctatttgaa atatagtatt
      661 gtttccatat ttagaataaa tgtacgaagt atttacaatg agcttaggat taattattcg
      721 taaccatcta attgttgatg tgatattatt cgaagtattt ttggaatttt cattaataaa
      781 aatatcactt ccgttatata ccgaaagaaa caacctattt tttgagttga ttttataatt
      841 aatttttgta ttaatatcat agaaaagagg aacttcaaaa ccttgtttac tccaattcat
      901 aaatatatcg agtacacttt ttcgaattgc aaaaaagtat gaagcagtat cttttttaat
      961 tgggccttca atatttgcat ctattgttaa tgggctaaaa ctaaattgac ctgtatttgc
     1021 attcatattt ccttctttca ttttnnnnnn nnnnaccgaa gaaattttac ctccaaattc
     1081 agccggtata ttactattat agaatgttgc atctttgagc atagtaggat ttattgccga
     1141 tacaaaacca tacatatgtg agatattata aattggggca tcatctatta ttatcatatt
     1201 ctgatcccta tttcctccac gcacatacat accagatgct ccatcgccaa tatttttgat
     1261 tcccggttgt aattgtaata cttttattac atcaggcaca ccaaatattg taggtgtttg
     1321 ttctatttgt tgtaaaggca ttttttttac aagtccagat agttcatatt ttgttgcaga
     1381 aacaattatt gtattaagtt ctatagatga tttttgtaaa aatacattat ggtgtatatg
     1441 tttaaatatg gtaatagtat caattgtaat actatatcct acatgagaaa attctaatat
     1501 gtgtactcct tcttcgagaa gtattgaata aaacccattt tcattggtta atgtagtttt
     1561 atttgtttgg taatcatata tcgatacacc tattagttct tcgccggtaa cagtatctct
     1621 tacaaatcct gaaattgtat acgagctttg acaaaatcct aaactgctat atattaaaaa
     1681 aaatagtata atatagtttt tcatattttg ttgtattaag taacccatgc ataatttgta
     1741 tgcatgggtt tttgcatttt attctattgt aaacaagcaa cttacttctc ctttattact
     1801 attgttatat gttaccctta ctactatcca ccctaaggta ttgctgggcc gaatagacca
     1861 caccatatca tatttatttt ttatttcaaa ttgttgatac ggttctacat ctaaataatt
     1921 cccgttatca tacactctta cccaagcatt attcaagcat agtaatgccc ctgattcatt
     1981 tctgattttt acagctaaag cattcctatg ttgccatata tcatgatgat aaggcaataa
     2041 gttgaagtcg cattttaaaa acgcttttga ttttattttt tttaagacat actttgatgt
     2101 tggaaattta aaatcatttg aaagaatttc aattagtaat gtttttgagg aatcaaaatg
     2161 attgaaatta ctttcttggc agatttcatt gttttcgata attggttttt gagattcgta
     2221 ttcttcaatt cctttattat tagcaatcag cataaaatca ttttctgacc attcatcaag
     2281 atataattta tcatctgatg aaatttttaa ttttactgca tttttattga ctgaatctac
     2341 aacaataagt tctttggagt attgctgttt ggtttgtata ttttcatctt tcttacatga
     2401 atataaaaat acactcaaca aacttgctga aataatatga aataatttag aaaaaattat
     2461 tgatttgatt tgatttgatt tgatttgatt ttcatagttt aaaa
//
LOCUS       DAOTOW010000310         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_32917_length_2481_cov_2.990932, whole genome shotgun sequence.
ACCESSION   DAOTOW010000310 DAOTOW010000000
VERSION     DAOTOW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32917_length_2481_cov_2.990932"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1373
                     /locus_tag="P5243_06295"
     CDS             288..1373
                     /locus_tag="P5243_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRS19092.1"
                     /translation="MKKVCVFLVSNIVGIVIYINMAYSQRIAIQSNSFVVNGKPIFLV
                     GANTPWDTWNDFGGNYNPQFWEDEFARLQKTGINSSRIWISCDNVGQPYIDVLGVIHK
                     PTDQFWQHMDDMMQKAQKYGIYIMATITSFDHFDKEKANYQQWRSMVHCSQQIQTYID
                     VFLLPLIARYESNPYFFSIDICNEPEWIHEHAKQGAIDWQYLQQFVGMCAAAVHKSQS
                     KVLVTVGSAAVKWGSNKYEGNKWSDGVLQKVTGDSLAYLDYWQVHYYEWINTYFSNPF
                     TMSPQDYLLNDRPCVIGETPGLNTIYGFPISYEEIYEKPYKLGYDGVFPWTSNGAGKG
                     DFGNLTTFGKGAHAFVQKYPELVQKIK"
     gene            1396..>2481
                     /locus_tag="P5243_06300"
     CDS             1396..>2481
                     /locus_tag="P5243_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HRS19093.1"
                     /translation="MSKQKEIGILSFKEKLSYGFGDLASVLYWQTFMLYFTFFYTDVF
                     IIPAAVAATLFLVSRIFDGITDTIMGMIADRTQTRWGKFRPYLLWFCVPFAIVGVLTF
                     TVPDFELTGKIIWAYVTFNLIMILYTIINIPYTALLGVISPNSAERTTVSSIKFIFAF
                     AAGIIVSATLLPMTQSLGGGNEALGWQRSFMVYGIAAIVFFLIAFKGTKERVLSPKTQ
                     KTSIKQDLKELVTNKPWGILLLTTITFILFVAVRGSVTVHYFKYIIGTQSIQLPFFGN
                     NTYDFNILTSAYNTVGQISSLLGVVAVSFLAKKFGKQRMFILFFIIAIVSTALVKFLT
                     AQQIGLIFILQIFGSFTGGPLSVLLWAM"
BASE COUNT          819 a          344 c          434 g          884 t
ORIGIN      
        1 tgataaaatt taattcataa tcaatgtgaa aatgaacagg aaaatcgaag actttatttt
       61 ttctaacaaa tactaataca cagtcatcat tagtaagtgg agtagattca tgaaaagccg
      121 atttcatacg taaaagtttg atgtaaatat ataataattt gataaaatac tattgaattt
      181 atatgttttt gtgttgctat ttttgttatc tgttatttaa tactattttt tatgcaatta
      241 caactaaaaa gtgtattttt attttttaat attcaaatga acgtttcatg aaaaaagtat
      301 gtgtgttttt agtatctaac attgttggca ttgtaatata tataaatatg gcatattcac
      361 aacgtattgc aattcaatca aattcatttg tggtaaatgg aaaacctata tttttggtgg
      421 gagctaatac tccatgggat acctggaatg attttggagg taattataat ccacaatttt
      481 gggaagatga atttgctcgt ttgcagaaaa ctggtattaa cagttcgcga atttggatta
      541 gctgtgataa tgtagggcaa ccatatattg atgtattagg cgtaattcat aaaccaaccg
      601 accaattttg gcaacatatg gatgatatga tgcaaaaagc acagaaatat ggtatttata
      661 ttatggctac tattacatct tttgatcatt ttgataaaga aaaagcaaat tatcaacaat
      721 ggagaagtat ggtgcattgc tcgcaacaaa tacaaacata tattgatgtt tttttattac
      781 ctcttattgc ccgatatgaa tcaaatccct attttttttc gattgatatt tgcaatgaac
      841 cggagtggat tcatgaacat gccaaacaag gtgcaataga ttggcagtat ttgcaacaat
      901 tcgtaggtat gtgtgctgct gccgttcata aatcgcaaag taaagtttta gttacggtag
      961 gtagtgctgc agtaaaatgg ggaagcaata agtatgaagg aaataaatgg agtgatggcg
     1021 ttttacaaaa ggtaaccggt gattcattag catatttaga ttattggcaa gtacattatt
     1081 atgaatggat aaatacatat tttagtaatc cgtttaccat gtcgcctcaa gattacttgc
     1141 tcaatgaccg accatgtgta ataggcgaaa caccgggatt aaatacaata tacggtttcc
     1201 ctatttctta tgaagaaata tatgaaaaac cttacaaatt aggatatgac ggagtattcc
     1261 cgtggacatc aaacggtgca ggaaaaggtg attttggaaa tcttactaca tttggaaaag
     1321 gagcacacgc atttgtacaa aaatatccgg aattagttca aaaaataaaa taataacctc
     1381 aaaaatttat attctatgtc aaaacaaaaa gaaattggta ttttatcatt taaagaaaaa
     1441 ctgtcatacg gttttggtga tttagcgtcg gtactttact ggcaaacatt tatgttgtat
     1501 tttacatttt tttataccga tgtatttata ataccggctg ccgttgcggc aacattattt
     1561 ttggtttcaa gaatttttga cggcattaca gatactatta tgggaatgat agccgataga
     1621 acacaaactc gttgggggaa atttcgacca tatttattgt ggttttgtgt gccatttgcc
     1681 atagtgggag tgcttacatt tactgttccc gattttgaac ttaccggtaa aattatttgg
     1741 gcgtatgtta cattcaatct tattatgatt ttatatacaa ttataaatat tccttatact
     1801 gcattacttg gagtaatttc accaaattct gctgaaagaa ctacggtgtc gtctattaaa
     1861 tttatttttg catttgcagc cggaattatt gtatctgcta ctttattacc aatgactcaa
     1921 tcattgggtg gtggaaatga ggctttaggc tggcagcgtt catttatggt ttatggaatt
     1981 gcagctattg tattttttct tatagctttt aaaggaacca aagaacgagt actttctccc
     2041 aaaactcaaa aaacttcaat aaaacaagat ttaaaagaac ttgtaactaa taaaccatgg
     2101 ggaatacttt tacttacaac tattactttt atattatttg ttgcggtgag aggaagtgtt
     2161 accgtgcatt attttaaata cattatagga acacaatcta tacaattacc tttttttggc
     2221 aataatacat atgattttaa tattcttaca tctgcatata acactgtagg tcaaatatca
     2281 tctttattag gtgttgttgc ggtaagcttt cttgctaaaa aatttggtaa acaacgtatg
     2341 tttattttat tctttataat agcaattgta agtactgctt tggttaaatt cttaaccgcc
     2401 caacaaatag gtcttatatt tattcttcaa atttttggat catttaccgg aggcccttta
     2461 agtgtgttgt tatgggctat g
//
LOCUS       DAOTOW010000311         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33184_length_2466_cov_3.072999, whole genome shotgun sequence.
ACCESSION   DAOTOW010000311 DAOTOW010000000
VERSION     DAOTOW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33184_length_2466_cov_3.072999"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2466)
                     /locus_tag="P5243_06305"
     CDS             complement(<1..>2466)
                     /locus_tag="P5243_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS19094.1"
                     /translation="TKGTLYVQASGGTVANDYSYLWSTGETNDTIKNLQPKIYTVSVT
                     DDNACVVSSSIDLTLVNDYKVSFEMSPVLCVGQSNGKAIATPVNGTAPYLYSWSNGVT
                     DSTIANVPAGKYIVDITDGNGCHAVDSIVVTTIPTVSIPNFGITETTCNGSIGSAWVK
                     PALGTAPYTYLWSTGETTDSIINKPVGIYSVTVTDKNGCTTFKNLFVKDTSSLKVKAS
                     TLQNKIRCAGRADGTATALATFGTSPYTYLWSNGATGTTASNLLAGTYYIEATDNNGC
                     KAVDSIKFIEENVFRIIIRDSSMITCYGGNDGWALVNAEGGIAPYSIVWNNGATTNLN
                     SGLTAGTYTATITDANSCTLQDQIVITQPPQLILTLDKVTDIKCKGICDATARGLVSG
                     GVAPYTYSWSNGETDSMAVALCGGKQYIDITDAHGCEIKDSVDILDIIPRLEVSFANV
                     LSSCGNADGETAAIPAGGVAPYTYLWNTGSTDTILQNIPAGAYSLKLKDSNGCTLDTI
                     TAVDDFTTMTATFTRQAITFCDVCNEKYKIEADNGAAPYTYEWSNGDTGAVADSLCYQ
                     QYAVTITDNNGCKKTEVLSVVKKSLDVNVVNKTNVDCYGGETGELEVKAINGISSNYY
                     YTWTNGNFGPKMSNVKAGTYGVTVNEDSSVCSITKYFTITQRPESRRFYVTDVPSYCK
                     DSTGIMHVEILAGSPPFTVQWGNGETGFVNSKAWNDYISVKITDLYGCEVTDSAKVDD
                     ISDFRLSENSRYLISCIGDSDGKLEVALDNGYAPFTYSWSHNSGLNAPLATGLAKGEY
                     TVTVIDNKKCKVSYTFDTLRDPQ"
BASE COUNT          757 a          497 c          358 g          854 t
ORIGIN      
        1 tttgtgggtc gcgtagggta tcaaatgtat atgagacttt acatttttta ttatcaatta
       61 ccgttactgt atattcacct ttagcaagac cggtagctaa aggagcattt aaaccggaat
      121 tatgactcca actataggta aatggagcat acccattgtc taaagctact tccagtttac
      181 catcagaatc acctatacat gaaattaaat atctgctatt ttcgcttaat ctaaaatcag
      241 atatatcatc aactttagca ctatctgtga cttcacaccc atataaatcg gtaattttta
      301 cagaaatata atcattccaa gcttttgaat taacaaatcc tgtttctcca ttaccccatt
      361 gaacagtaaa tggtggtgaa cctgctaata tttcaacgtg cataattccc gtagaatctt
      421 tacaatatga aggtacatcg gttacataaa atctgcgtga ttcaggtcgt tgagtaatag
      481 taaagtattt tgtaatacta catacactag aatcttcatt tactgtaaca ccataagtgc
      541 ctgctttaac atttgacatt tttggtccaa aatttccgtt tgtccaagta taatagtaat
      601 tagaacttat tccatttatt gcttttactt caagttctcc tgtttctcct ccataacaat
      661 caacatttgt tttgtttaca acatttacat caagagattt ttttactacc gataatactt
      721 ctgttttttt acaaccatta ttatcagtaa tagttacagc atattgttga tagcacagtg
      781 aatcagctac agctccggta tctccattac tccactcata cgtatatggt gccgcaccat
      841 tatctgcttc aattttatat ttttcattac aaacgtcaca aaaagtaatt gcttgtctgg
      901 taaatgtagc tgtcatagta gtaaaatcat caacagctgt tattgtatct aaagtacaac
      961 cgttactatc tttgagtttt aaagaatatg cacctgcggg tatattttgt aaaatagtat
     1021 ctgtacttcc cgtattccat agatatgtat atggagctac acctcctgcc ggaattgccg
     1081 cagtttctcc atcagcattt ccacacgaag ataaaacatt tgcaaaacta acttccaaac
     1141 gtgggattat atcaagtata tcaacactat ccttaatttc acatccatgt gcatctgtta
     1201 tatcaatgta ttgtttacct ccacataatg ctaccgccat agaatctgtt tcaccattcg
     1261 accacgaata tgtatatggt gctacaccac ccgaaacaag tccacgtgca gttgcatcac
     1321 aaattccttt acatttaata tctgtaacct tgtctaaggt aagtattaat tgtggtggtt
     1381 gtgttataac tatttgatct tgtaaagtac atgaatttgc atcggtaata gtcgctgtat
     1441 acgtccccgc tgttaacccc gaatttagat ttgttgttgc accattgttc cacactatag
     1501 aatatggtgc tataccacct tcggcattaa ctaatgccca tccatcatta cctccataac
     1561 aagtaatcat agacgaatct cgtataataa ttcgaaatac attttcttct ataaatttaa
     1621 tagaatcaac cgctttacaa ccattattat cagtagcttc tatataatat gtgcctgcaa
     1681 gtaaatttga tgcagtagtt ccggttgctc catttgacca tagatacgta tagggtgatg
     1741 ttccaaatgt tgctaatgca gtagcagttc catcagctct acctgcacat cgtattttat
     1801 tttgtaacgt tgacgctttt actttaagac ttgatgtgtc ttttacaaat aaatttttaa
     1861 acgttgtaca tccattttta tctgtaactg taacagaata aattccaaca ggtttgttta
     1921 ttatagaatc agttgtttct ccggtactcc acaaataggt ataaggtgct gttcctaatg
     1981 ccggtttaac ccatgcacta ccgattgatc cattacatgt agtttcagta ataccaaaat
     2041 ttggaatact taccgtaggt attgttgtta ctacaatact atccacagca tgacatccat
     2101 taccgtcggt aatatcaaca atatatttac ctgcaggaac atttgctatt gtagaatcgg
     2161 taacaccatt tgaccaacta tataaatatg gtgccgttcc atttaccggt gtagctatag
     2221 ccttaccgtt tgactgcccc acacacaata cgggtgacat ttcaaatgat actttgtaat
     2281 catttactaa ggttaaatca atagaacttg aaaccacaca agcgttatca tctgtaacag
     2341 aaacagtata aattttaggt tgtagattct ttattgtatc attagtttca cccgtactcc
     2401 ataaatatga ataatcattt gcaactgttc cacctgatgc ttgaacatat aaagtacctt
     2461 ttgtaa
//
LOCUS       DAOTOW010000312         2461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33280_length_2461_cov_3.166667, whole genome shotgun sequence.
ACCESSION   DAOTOW010000312 DAOTOW010000000
VERSION     DAOTOW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33280_length_2461_cov_3.166667"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="P5243_06310"
     CDS             <1..472
                     /locus_tag="P5243_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19095.1"
                     /translation="FCFFVTHVFSQQEYNQSVGLNVGIGVSSASVGPVLLDGVGTCVG
                     LSYLHDIFNSRFRIAPVFTIGRFMNDESNAQNWYRAQSMYINAECDVLRYRFFNTTLF
                     GGGGINMGSGNIDNIGTVGYSYFGYSYGLQFRFEPEQSPFSIVLQPVSIHNGFS"
     gene            complement(533..>2461)
                     /locus_tag="P5243_06315"
     CDS             complement(533..>2461)
                     /locus_tag="P5243_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19096.1"
                     /translation="ERTLRPLLFICTVFLCIGVYTCFSQDLQTIATDVYSFSAIDYSV
                     QQIEFLHDKMCNPIDINSIQESDLSQFCFLTPFEQKSIYEFVIANKPLSSIYELHFVL
                     GLSVEKAELLSHFMIVKPQSCNHTLEELIHNGTHSIIGISQFPIHIIDSSSTNNNYLG
                     NASKQAWRYRYASNGSLNWGVTLKKDSGEPVSCKQPNIYDYASAYIQYKPNGIIRNIT
                     IGDYELQIAQGLLQWQGGYFGKHIRSQHSKTHCTLSKHSSGNETDLYRGIAFQLSHNS
                     FMLTPFFSNMYTDGTLNSDSSNFILYNTGYHRTMREIELSNTIHHTAQGCHIQYSNFH
                     NTYSTAIVQHSLTYANTIISKIFVNAAYSYTLKQSQIFTESAFDGKSTAHTIGIQTSV
                     NHDITLLSSARYYQPNYYNIYSQSLSEQSQIHNEQGIFTSVICPISKFITFQCVHDYY
                     YIPNCRYFVNAPTRGNELYVKLQYKNWEGVTLFYAWSNGYKTAQNITERYNEIYTHIT
                     QFQKLYFVIPINSVLLSKTTIAQSLAENQRGTLVCQDLQYKPTKTCTIRIRYAHYSAP
                     YNARIYAWEDNVKYSITSHQYFNKGSHWYSLIEWQIHRMFRIEFKLSHTAIHKQLTVS
                     ETTPATLLCNIAVRYTIH"
BASE COUNT          741 a          328 c          474 g          918 t
ORIGIN      
        1 attttgtttt tttgtaactc atgtgtttag tcaacaagaa tataatcaat cggttgggct
       61 caatgtgggt ataggtgttt caagtgccag tgttggccct gtattgttag atggtgtagg
      121 aacatgcgtt ggtttatcgt atttgcatga tatattcaat tcgcgattta gaatagctcc
      181 ggtatttaca atcggacgtt ttatgaatga tgaaagtaat gcgcaaaatt ggtatagagc
      241 acaatcgatg tatataaatg ccgaatgtga tgtgttgcga tatagatttt ttaatactac
      301 attgtttggc ggcggtggaa taaatatggg tagtggtaat attgataata taggtactgt
      361 tgggtattcg tattttggat atagttatgg tttacaattt agatttgaac ccgaacagtc
      421 tcctttttct atagttttgc aaccggtatc aatacataat ggattttctt aaggtgaatt
      481 ttcaaatatg atgtcaataa gtttgggcgt tgaattgtat tttaaataat tcttaatgta
      541 tagtatatcg tactgcaata ttacagagca gtgttgccgg tgttgtttct gatactgtta
      601 attgtttatg aatagctgtg tgcgatagtt tgaattcaat tctaaacatt ctatgtattt
      661 gccattcaat taaactatac cagtgcgatc ctttattgaa gtattgatgt gaggtaatac
      721 tgtattttac attgtcttcc catgcataaa tacgagcatt atacggtgca gaataatgag
      781 catagcgtat tcgaatagtg catgttttgg taggtttata ttgtaaatct tggcatacta
      841 atgttcctcg ttggttttct gctagacttt gagcaatggt ggttttactt aaaagaacag
      901 agtttatagg tataacaaaa tacaattttt gaaattgtgt aatgtgtgtg taaatttcat
      961 tgtatctttc ggtaatattt tgggctgttt tgtatccgtt gctccaagcg taaaatagtg
     1021 taacaccttc ccaatttttg tattgcagtt ttacatataa ttcgtttcct cgtgtagggg
     1081 catttacaaa atatcggcaa tttggaatat aataataatc atgaacacat tgaaaagtta
     1141 taaatttact aataggacat ataacgcttg taaatattcc ttgttcattg tgtatttgtg
     1201 attgttcgct taagctttgt gagtatatgt tataataatt tggttgataa tatcgagccg
     1261 atgataaaag tgttatatcg tgatttacac tagtttgtat tcctatagta tgggcagttg
     1321 attttccgtc aaatgccgat tctgtaaata tttgtgattg ttttaaagta taactatacg
     1381 cagcgtttac aaatattttt gaaattattg tatttgcata cgtaagtgaa tgttgaacaa
     1441 tagcggttga atatgtgtta tgaaaatttg agtattgtat atgacaccct tgagcggtat
     1501 gatgaattgt gtttgaaagt tcaatctcac gcatggttct atgataccct gtattatata
     1561 gtataaaatt cgaagagtct gaatttaaag ttccatcggt atacatattt gaaaaaaatg
     1621 gagtaagcat gaatgaattg tgggagagtt ggaatgcaat tcctctatat aaatctgttt
     1681 cgttacctga gctgtgtttt gatagagtac agtgagtttt actgtgttga ctgcgaatgt
     1741 gttttccaaa atatccgcct tgccattgga gaagtccttg tgcaatttgc aattcatagt
     1801 cgcctattgt aatatttcta atgataccat taggtttata ttgtatatat gccgaagcat
     1861 aatcatagat attaggttgc ttacatgaaa ccggttcgcc actgtctttt tttaatgtaa
     1921 ctccccaatt taatgaaccg tttgaagcat acctatatcg ccatgcttgt ttacttgcat
     1981 ttcctaaata attattattg gtgctgctac tgtcaataat atgaataggg aattgactta
     2041 taccgattat gctatgagtg ccattgtgta taagctcttc taatgtgtgg ttgcaggact
     2101 gaggtttcac aatcataaaa tgtgataata attctgcttt ttcaactgac aaacctaaaa
     2161 caaaatgcag ttcataaatg ctgctcaagg gtttgtttgc aattacaaat tcataaatac
     2221 ttttttgttc aaagggtgtt aaaaaacaaa attgtgataa atcgctctct tgaatggagt
     2281 ttatatcaat agggttacac attttatcat gcaaaaattc aatttgttgg acactgtaat
     2341 ctatagccga aaaactataa acatcggttg caattgtttg taaatcttgt gaaaaacaag
     2401 tatatactcc aatgcaaaga aaaactgtgc atataaataa taaaggtctc aatgtgcgtt
     2461 c
//
LOCUS       DAOTOW010000313         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33334_length_2458_cov_3.455264, whole genome shotgun sequence.
ACCESSION   DAOTOW010000313 DAOTOW010000000
VERSION     DAOTOW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33334_length_2458_cov_3.455264"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="P5243_06320"
     CDS             <1..929
                     /locus_tag="P5243_06320"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HRS19097.1"
                     /translation="LQRNCDKYGALYAYETTVNTDKVQMKQISESQKIDKDKDGVLDY
                     INTLRSTTIDQLVAKYYEAAATQAETLIEAETKKQISSFLLQSKIQEAIRSSLLQSLY
                     EHDDFVDIASVEQKVNVAIAQAIIDAVADANLSAFISSQKLQQIAFTVSEPVAANVAN
                     DIAKQISTAIIAEYKQMIQSGTMQYVQGICPDGYYIPSDVDWMIFELALGMSSQDLTK
                     YGETVTNRGASAQVPNKMVKDHGFEYGGYMSINGTYAQLGEAGVYWSSTIGTDEKGDY
                     VWVRQIDTSYTGVLRYKHYEKSGLSIRCFKKP"
     gene            1096..>2458
                     /locus_tag="P5243_06325"
     CDS             1096..>2458
                     /locus_tag="P5243_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRS19098.1"
                     /translation="MLHYSFGQSSVDTLKIYKKAKSAPFYVSKNPQKLSRYLTSGLQN
                     DAEKVLAISYWICNNISYKYMGQISRSVEIKSSHKILQQKKATSIEYAKLFKELCAVV
                     NIPSEIVHGYVKDFDFFPGDTLYRAEYAWNVVKISNEWQIIDLTLAANDLEPDGTPFS
                     KFMWAVLRKPTTVKMKPVKRYSPQWICVPPHKAIYTHMPILPMFQLLEYTITIDVFMR
                     GDSIINQYVGRYPQKTRYYFDLEKYVTLNDVDKYVYVAKNTLLENPFAHFNAAIYYYF
                     AVEHFKRKNYLEEKGRLFAPQDEIRPMLVYSNYADSLFVLSVTDDVKELKAFQARSEK
                     WKRDLAEYNKIHSANSQELIKRYMKQIQNIKRIKTQNDAIVSYIQKNKSIVSDKTIID
                     QPRPMVQKNADMYEAIALKSQVDSMGIVIKKIQKYLDNQIGFVSKYDAQKALQNEQNA
                     LKIN"
BASE COUNT          931 a          332 c          405 g          790 t
ORIGIN      
        1 atttacagcg aaattgtgat aaatatggtg cattatacgc ttatgaaacg acggtaaata
       61 ctgataaggt acagatgaaa caaatatcag aatctcaaaa aatagataaa gataaagacg
      121 gagtgcttga ttatataaat actcttagaa gcactacaat agatcaatta gtggctaaat
      181 attatgaggc tgctgcaaca caagcagaaa cgttgattga agcagaaaca aaaaaacaaa
      241 ttagttcatt tttacttcaa tcaaaaattc aagaagctat tcgttcgtca ttacttcagt
      301 cattatatga gcatgatgat tttgttgata ttgcatctgt tgaacaaaaa gtaaatgtag
      361 caattgctca agctattatt gatgctgtag ctgatgctaa tttgagtgcg tttatatcat
      421 ctcaaaagtt acaacaaatt gcttttactg tttcagaacc cgtagcagca aatgttgcaa
      481 atgatattgc aaaacaaata tcaacagcaa ttattgccga gtataaacaa atgattcaat
      541 caggaacaat gcaatatgtt caaggtattt gtcctgatgg ttattatatt ccatctgatg
      601 ttgattggat gatatttgag ttagctctag gaatgtcatc tcaagattta acaaaatatg
      661 gtgaaacagt aacaaataga ggagcatcag ctcaagtgcc taataaaatg gttaaagatc
      721 atggatttga gtatggtggg tatatgagta taaatggaac atatgcccaa ttaggtgaag
      781 ccggggtata ttggtcttca actattggta ccgatgaaaa aggtgattat gtatgggtgc
      841 gtcaaataga cacaagttat accggagtat tacgatataa acattatgag aaatccggat
      901 taagtatacg atgtttcaaa aaaccataat tatatataaa aacctcaaat tcaattgaat
      961 ttgaggtttt ttttgtcttt tttgcttttt ctattaaaaa tacgtatatt cacataatat
     1021 ttagaaaact atttaagaat atattcaatg gggaaaatgc ggatactttt ttttctttgt
     1081 ctactttttg tacaaatgct tcattacagt tttggacaat catcggtaga taccttgaaa
     1141 atatataaaa aagctaaatc agctcctttt tatgtttcaa aaaatcccca aaaattgtca
     1201 agatatctta catcgggatt acaaaacgat gcagaaaagg ttttagcaat atcatattgg
     1261 atttgtaata atatttcata taaatacatg ggacaaattt ctcgttctgt tgaaattaaa
     1321 agttctcata aaatattaca gcagaaaaaa gcaacaagta tagaatatgc taaacttttt
     1381 aaagagttat gtgctgttgt taatattccg tcagaaattg ttcacgggta tgtaaaagat
     1441 tttgattttt tccctggtga tacattatat agagcagaat atgcttggaa tgttgtaaaa
     1501 atttctaatg aatggcaaat tatagattta actcttgctg caaatgattt ggaacccgat
     1561 ggtacaccgt tttcaaaatt tatgtgggca gtattacgta aacctacaac tgtaaaaatg
     1621 aaaccggtaa aacgctattc acctcaatgg atttgcgttc ctcctcataa agcaatatat
     1681 actcatatgc ctatattacc aatgtttcaa ttgttggaat atactattac aattgatgtt
     1741 tttatgcgag gtgattctat tataaatcaa tatgtaggaa gatatccaca aaaaacaaga
     1801 tattattttg atttagaaaa atatgttacg ttgaacgatg ttgataaata cgtgtatgtg
     1861 gcaaaaaaca cattacttga aaatccattt gcacatttta atgccgctat ttattattat
     1921 tttgctgttg aacatttcaa aagaaaaaat tatttagaag aaaaaggacg gttgtttgct
     1981 ccacaagatg aaattcgacc tatgttagtt tattctaatt atgccgattc attgttcgtg
     2041 ctttctgtta cagacgatgt taaagaatta aaagcttttc aagcaagaag tgaaaaatgg
     2101 aagcgtgatt tagcagaata taataaaatt cattcagcaa atagtcaaga gttaattaag
     2161 cgttatatga agcaaataca aaatataaaa cgcataaaaa ctcaaaacga tgcaattgta
     2221 tcatatattc aaaaaaataa atctattgtt tcagataaaa caattataga tcaaccaaga
     2281 ccaatggttc aaaaaaatgc tgatatgtat gaagcaatag ctttaaaatc tcaagttgat
     2341 tctatgggaa tagttatcaa aaaaattcaa aagtacttag ataatcaaat agggtttgta
     2401 tcaaaatatg atgcacaaaa ggcattgcaa aatgaacaaa atgcactgaa aataaacc
//
LOCUS       DAOTOW010000314         2455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33388_length_2455_cov_3.330417, whole genome shotgun sequence.
ACCESSION   DAOTOW010000314 DAOTOW010000000
VERSION     DAOTOW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33388_length_2455_cov_3.330417"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..1064
                     /gene="folE"
                     /locus_tag="P5243_06330"
     CDS             429..1064
                     /gene="folE"
                     /locus_tag="P5243_06330"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRS19099.1"
                     /translation="MEIYNNGLTIRHQSNYQRVEQYNDDITQKLAELYKQALELLGED
                     SQREGLLKTPERMAKAMQFLTQGYNTDPKEILQSAMFKEEYKQMVIVKDIEIYSMCEH
                     HMLPFIGKAHVAYIPNGYITGLSKIARVVEVYARRLQVQERLTVQIRDCIQETLNPLG
                     VAVIIEAHHLCMQMRGVQKQNSATTTSAFTGAFTKKETREEFVHLVGTQLH"
     gene            1119..2000
                     /gene="fabD"
                     /locus_tag="P5243_06335"
     CDS             1119..2000
                     /gene="fabD"
                     /locus_tag="P5243_06335"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRS19100.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYETYPVAKEMFEKANQILGFRITD
                     LMFAGTDEDLKQTKVTQPAIFLHSVILAKVLGDTFQPNAVAGHSLGEFSALVAAGALS
                     FEDGLVLVSKRAMAMQKACEQNPSTMAAILNLDDAVVEEVCASIDDVVVAANYNCPGQ
                     LVISGTNAAIDKACELLLAKGAKRALKLPVGGAFHSPLMEPARVELEAAIAATNIQSP
                     ICPIYQNVNAMPQTDATVIKQNLIAQLTAPVKWTQTVQNMLADGIDSFIEVGPGKVLQ
                     GLVKKVNKDVEAISAEI"
     gene            2010..>2455
                     /gene="dtd"
                     /locus_tag="P5243_06340"
     CDS             2010..>2455
                     /gene="dtd"
                     /locus_tag="P5243_06340"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRS19101.1"
                     /translation="MKAVIQRVSKASVTIHGRVKSTIGTGFLVLLGIEHADTVEDAQW
                     LSQKIAHIRIFNDEQGLMNLDLEAVKGNVIVVSQFTLHASTKKGNRPSYIKAAKPEHA
                     ISLYENFIHSLQQQISGTVLTGEFGADMKVDLCNDGPVTIIIDSKN"
BASE COUNT          936 a          391 c          433 g          695 t
ORIGIN      
        1 aattacatcg ggagtatttt gaatacaata tttggttaat gaatataaag tagcataact
       61 ccctacacct gaaactaaaa ccgaaatagt atggttgcta tctttaggta tggtaaaatt
      121 atgcgcttca acttctgaag caaatacaac aactatattc atgcttatat ttttattgta
      181 aaaatacaca ttttttgatt gttatgagaa aaaagtatca ttcattgaca cataataaaa
      241 actagaatta cagagcaaaa caaaatacat gcacaataat tctcaatttt attgcaatgt
      301 atcattctat ttgctgtcca atagaaaaaa aataaaattt gtaaatatta aaaaaactac
      361 cttatttttg ccaaaacctc aatttataaa aaaatgagga atggttcgta aggacaaaat
      421 aattaactat ggaaatttac aacaacggat taacaatccg gcatcaaagt aactatcaac
      481 gagtggaaca atacaacgac gatattacac aaaaattagc agagctctat aaacaagcac
      541 ttgaactgtt aggcgaagat tcgcaacgag aaggactact taaaactcct gaacgaatgg
      601 caaaagcaat gcagtttctt acgcagggat acaataccga ccctaaagaa atactgcaat
      661 ctgcaatgtt caaagaagaa tataaacaaa tggtaattgt aaaagatata gaaatttaca
      721 gtatgtgcga acatcatatg ttgcctttta taggtaaagc tcatgtagca tatataccaa
      781 acggatacat tacaggatta agcaaaattg ccagagtagt cgaagtatat gcccgcagac
      841 tgcaagttca ggaacgttta acagtacaaa ttcgtgattg tatacaagaa acactcaatc
      901 cacttggagt tgctgtaatt atagaagcac accatttatg tatgcaaatg cgtggcgttc
      961 aaaaacaaaa ttcggcaact actacttcgg catttaccgg tgcgtttaca aaaaaagaaa
     1021 cacgcgaaga atttgttcat ttagttggta cacaattgca ttgatagaat taagagttac
     1081 ataatgaaaa taataaatat taactaaaca acaacaatat gaaagcttac gtatttcccg
     1141 gtcaaggggc acaatttgta ggaatgggta aagatttata tgaaacctat ccggtagcaa
     1201 aagaaatgtt tgaaaaagca aatcaaatat taggatttcg cataacagat ttaatgtttg
     1261 caggaaccga tgaagattta aaacaaacaa aagtgactca accggctata tttttacatt
     1321 cagtaatatt agcaaaagta ttaggagata cattccaacc aaatgctgtt gcaggtcatt
     1381 cattaggtga attttcggct ttagtagctg caggtgcttt atcgtttgaa gatggattag
     1441 tattagtttc aaaacgtgca atggcaatgc aaaaagcatg tgaacaaaat ccttctacta
     1501 tggcagcaat tttaaattta gacgatgctg tagttgaaga agtttgtgct tcaatagatg
     1561 acgttgtggt agcagctaat tacaactgcc cgggacagct tgtaatatcg ggaacaaatg
     1621 ctgctattga caaagcatgt gaattattgc ttgcaaaagg agcaaaacgt gcattaaaat
     1681 taccggtagg cggagcattc cactcacctc ttatggaacc tgctcgtgta gagttagaag
     1741 ctgcaattgc cgctactaat atacaatcac ctatatgccc aatttatcaa aacgtaaatg
     1801 caatgccaca aacagatgct acggtaatta aacaaaattt aattgcacaa cttacagcac
     1861 cggtaaaatg gactcaaact gttcaaaata tgcttgccga cggtattgat tcttttatag
     1921 aagttggtcc gggtaaagta ttacaaggat tagtaaaaaa agtaaacaaa gatgtagaag
     1981 caattagtgc tgaaatataa attaaaccat tgaaagcagt tattcaaaga gtatctaaag
     2041 catcggttac catacatggt agagtaaaat ctaccattgg taccggtttt ttagttttat
     2101 taggtataga acatgctgat actgtagaag atgcgcaatg gctttctcaa aaaatagctc
     2161 atattcgtat attcaatgat gaacaagggc taatgaattt agatttagaa gctgtaaaag
     2221 gtaatgtaat agttgtaagt cagtttacat tacatgcaag taccaaaaaa ggtaataggc
     2281 catcatatat taaagcagca aagcccgaac atgccatctc tctgtatgaa aattttatac
     2341 attccttaca acaacaaata tcaggtactg ttctaaccgg agaatttgga gctgatatga
     2401 aagtggattt atgcaatgat ggtccggtaa ctattataat tgattcaaaa aataa
//
LOCUS       DAOTOW010000315         2450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33486_length_2450_cov_3.178706, whole genome shotgun sequence.
ACCESSION   DAOTOW010000315 DAOTOW010000000
VERSION     DAOTOW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33486_length_2450_cov_3.178706"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="P5243_06345"
     CDS             <1..406
                     /locus_tag="P5243_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS19102.1"
                     /translation="LFQNELEITKKYLEIQQTRYGDNLQVEITVDPNTLQFLLPPMAL
                     LTIVENCIKHNIISSEKPLTIYISTLPNVIRISNNLQKRNDVYCSGQGLQNVIQRYSF
                     VCPQKVVVTEIQDFFIVEIPLINNIHDTNSNN"
     gene            384..1133
                     /locus_tag="P5243_06350"
     CDS             384..1133
                     /locus_tag="P5243_06350"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS19103.1"
                     /translation="MIRTVIIEDEILARQVLKTMLQQIPEVDIVKELESVSQAVTWFS
                     QNTVPDLLLVDVHLADRISFSIFEQIHIQCNVIFITAYDEYALRAFELQSIDYLLKPI
                     TLEALQAAINKLKRMTGIQNYYFLQKISEQFNKKMYKERFVVHIGAKIYTFLIQDIVC
                     FYVFEKGTYLFTKQAKSYPIDYSLDAIENSINPSDFFRVNRQYIVSFASIREVIKMSN
                     NAIQIDIEPSVGNKIMVSTHKAAAFKNWLEQ"
     gene            complement(1144..1680)
                     /locus_tag="P5243_06355"
     CDS             complement(1144..1680)
                     /locus_tag="P5243_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS19104.1"
                     /translation="MIYHKILETTDIRLRALEPEDIDLLYTWENNTDVWIISSTIAPF
                     SRNILHKYIENSHLDIYTVKQLRLMIDCLHTHTTIGAIDLYDFDPHNKRAGVGIYISP
                     EYAHKGYGEKSLKCLLNYCKDVLFLHQVYSEITEINKPSLALFTKVGFKENGTKKEWI
                     QTPDGYIDEYILQYIFEE"
     gene            1905..>2450
                     /locus_tag="P5243_06360"
     CDS             1905..>2450
                     /locus_tag="P5243_06360"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRS19105.1"
                     /translation="MAEKQKTVQKEVSFSGKGLHTGKVVNVTILPAEPGFGIKFKRVD
                     LPENPVIPALAENVVETARGTVIGVKDATVSTIEHLLAAMCALHIDNAEIQVDGPEVP
                     IMNGSAKPFYDILLQAGVQEQDAEREYYEITKKIEYIEPERGVEIVAYPCDTFKVDVK
                     ISYDSPLLKNQFASLSSLQDFY"
BASE COUNT          883 a          339 c          384 g          844 t
ORIGIN      
        1 gttgtttcaa aatgaattgg agattactaa aaagtatctc gaaattcaac aaacacgata
       61 cggagataat ttgcaagttg aaattactgt tgaccccaat acgttacaat ttttactgcc
      121 gcctatggct ttgcttacta ttgttgaaaa ttgtataaaa cataatataa tttcttctga
      181 aaaacctctt actatttata tttcaacctt gccaaatgta attagaattt ctaataattt
      241 acaaaaaaga aacgacgtat attgttcagg tcaaggactt caaaatgtaa ttcagcgtta
      301 ttcttttgtt tgccctcaaa aggttgtagt aactgaaata caagattttt tcattgtcga
      361 aatacccctt ataaataata tacatgatac gaacagtaat aattgaagac gaaattttag
      421 cgcgacaggt tttaaaaacc atgttacaac aaattcctga agttgatatt gtaaaagaac
      481 tcgaaagtgt atcacaagct gttacgtggt tttcacaaaa tacagtaccc gatttactgt
      541 tggttgatgt acatttggct gatagaataa gtttttctat tttcgaacaa attcatattc
      601 aatgtaatgt aatttttatt actgcatatg atgaatatgc tcttcgtgcc tttgaattac
      661 aaagtataga ttatttatta aaacctatca cgttagaagc attgcaagct gctatcaaca
      721 aattaaaacg aatgacaggt attcagaatt attacttttt gcaaaaaata tcagaacaat
      781 tcaataaaaa aatgtataaa gaacgatttg ttgttcatat aggagcaaaa atttatacat
      841 ttttaataca agatatcgta tgtttttatg tatttgaaaa aggaacatat ctatttacca
      901 aacaagcaaa atcgtatcct atagattatt cacttgatgc tattgaaaat agtattaacc
      961 caagcgattt ttttcgggta aataggcaat atatagtttc atttgcaagc attagggaag
     1021 ttataaaaat gtcaaacaat gcaattcaaa ttgacataga accaagtgtt ggcaacaaaa
     1081 ttatggttag cacgcataaa gcggcagctt tcaaaaattg gttagaacaa taaaaaaata
     1141 atattattct tcaaaaatat attgtaaaat atattcatca atatatccat caggagtctg
     1201 tatccattct tttttagtac cgttttcttt gaatcctact ttagtaaata atgctaaact
     1261 tggtttattt atttctgtaa tttcactata tacttgatgt agaaagagta catctttaca
     1321 atagttgagt aaacatttga gagatttttc tccatatcct ttgtgtgcat attcaggtga
     1381 tatatatatg ccaactcctg cgcgtttatt atgaggatca aaatcataca aatcaattgc
     1441 tcctatagtt gtatgtgtat gcaaacaatc tatcattaaa cgcaattgtt taacggtata
     1501 aatgtctaaa tgactgtttt ctatgtattt atgtagtatg tttctagaaa aaggagcaat
     1561 ggtagaactg ataatccata catcagtatt attttcccac gtatacaata aatcaatatc
     1621 ttcgggttct aaagcccgta aacgaatgtc ggtagtttcg agtattttat gataaatcat
     1681 atttgtattt tttttggaaa caattttaca tatattcgtt tctgattgta aaatacttgt
     1741 atttttgtaa aaaaaatgga actaagtaat ttttatgtaa aggtatatta ttgatgccgt
     1801 atattcaatt ttttgctgtt attttttttt atataatagc attgaaaata aaagcatacg
     1861 tgtatctttg taattaatta cacaaaaaat tatacgaata ttctatggct gaaaagcaaa
     1921 aaacagtaca gaaagaggtt tctttcagtg gaaaaggatt acatacggga aaagttgtaa
     1981 atgttacaat actgcctgca gaacctggtt ttggcataaa gtttaaacgt gttgatttgc
     2041 cggaaaatcc tgtaatacct gccttggcag aaaatgtagt agaaacagca cgtggaacgg
     2101 taataggagt aaaagatgct acagtaagta ctatcgagca tttattagca gcaatgtgtg
     2161 cattacacat tgataatgcc gaaattcaag ttgatggtcc tgaagttcct attatgaacg
     2221 gaagtgcaaa accattttat gatattcttt tacaagctgg tgtacaagag caagatgcag
     2281 aacgagaata ttatgaaatt actaaaaaaa tagaatatat agaaccggaa cgtggagttg
     2341 aaatagtagc atatccgtgt gatacattta aagttgatgt taaaatcagt tacgattcac
     2401 cattgttaaa aaatcaattt gcttcattat cgtcattgca agatttttat
//
LOCUS       DAOTOW010000316         2450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33496_length_2450_cov_2.232568, whole genome shotgun sequence.
ACCESSION   DAOTOW010000316 DAOTOW010000000
VERSION     DAOTOW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33496_length_2450_cov_2.232568"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="P5243_06365"
     CDS             <1..1381
                     /locus_tag="P5243_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS19106.1"
                     /translation="HWQGQNPWIDKNKVEIPNFIESVVKNSWFYYKLDARFKGNQDSI
                     QYYLHKKEPMKLFSWKGPIDTVCSFIEATNYLKRLLHSGFVAMDPETGAIKAYVGGID
                     FNYFQYDNVKSMRQPGSTFKSIVYATALDKGMSPCDSIPDVPQVIKYIEKGEEKVWIP
                     QNSDRTFVGRNVALKYAFAHSLNTISVQLTQKFGIETVIDYAHKLGIKSPLDTVPSVC
                     LGSSDVTLLELTTSYCPFLNGGYRVTPMFVTRVEDREGNVLVEFKPEKEKILGEETVF
                     LMQQMFLASLSEPYGTTQNLFSYDLFKYKTDFGGKTGTSSNYSDGWFVGVSPKLVAGT
                     WVGAEERCVHFRSSHLGEGGKTALPTFGLFMEKVMRDTAYTYLRAQFPRYLKGLKDRP
                     YSCYTKYVPRDTVDSVEVDEMGMDSIKVVVPQPVINTSKAEVKQPETQSVEAVENKRP
                     RLFQRREKE"
     gene            1404..>2450
                     /locus_tag="P5243_06370"
     CDS             1404..>2450
                     /locus_tag="P5243_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRS19107.1"
                     /translation="MNYSQVLPLRKRHKLGGMKELLILALPIIASQSCETIMVFTDRV
                     FLSKLSSEQMNAATGGGISSFLLLTFFIGLIGFSTALTAQFFGAKKYEQCSITLVQSV
                     FISIFAYPIIIFALPLLYWYFEVTHISAGQMMFQKQYVQILVFGSIISLIRHSLSCYF
                     SGIGRTKIVMMAAIFTMVVNIGFNYVFIFGHLGMPAMGIRGAALGTILSALCGVAVMS
                     YVFFSKKNVQKFQVYKSFKFHAKTFKTLLRYGTPAGTEFFVNFIAFSLLVFLFHSYNE
                     QTATAASIMFNWDMVTFVPLVGIEIGVTSMVGRYVGAKKLHVAERATYTAVRLGWFFS
                     AFVVLMFVFIPHVLV"
BASE COUNT          775 a          335 c          479 g          861 t
ORIGIN      
        1 gcattggcaa gggcaaaatc catggattga taaaaataaa gttgaaatac ccaattttat
       61 tgaaagtgtg gtaaaaaatt catggtttta ctataaactc gatgctcgtt tcaaaggtaa
      121 tcaagattct atacaatact atttgcataa aaaagaacct atgaaattat tttcatggaa
      181 aggacctata gatactgtat gtagttttat agaagcaact aattatttaa aacgtttgtt
      241 gcatagtgga tttgtagcaa tggatcccga aactggtgct ataaaagcat atgttggtgg
      301 gatagatttt aattattttc aatacgataa tgtaaaatct atgcgtcaac ccggttctac
      361 atttaagtca atagtgtatg ctacagcatt agataaagga atgtcaccat gcgatagtat
      421 acccgatgtg ccgcaagtaa ttaaatatat tgagaaagga gaggaaaaag tatggattcc
      481 tcaaaattcg gacagaacat ttgttggtag aaatgtagct cttaaatatg cgtttgcaca
      541 ttcattaaat actattagtg tgcaacttac ccagaaattt ggaatagaaa cagttattga
      601 ttatgctcat aaattaggta taaaatcacc gttagataca gttccttcag tatgtctagg
      661 ttcaagtgat gttacattgt tggaacttac aacttcgtat tgtccgtttt taaacggggg
      721 atatagagtt acgcctatgt ttgttactcg tgttgaagac cgtgagggca atgtattggt
      781 agagtttaaa ccggaaaaag agaaaatact tggtgaagaa acagtatttt taatgcaaca
      841 aatgtttttg gcatcacttt cagaaccata tggtacaact caaaatttgt tttcgtacga
      901 tttattcaaa tacaaaacag attttggagg taaaaccgga acttcatcaa attattccga
      961 tggttggttt gttggggttt cgcccaaact tgttgcaggt acttgggtag gtgctgagga
     1021 acgatgtgtt cattttcgtt cgtcgcattt aggtgaagga ggaaaaacag ctcttccaac
     1081 gtttgggttg tttatggaaa aagtaatgcg tgatactgca tatacatatt tgagagctca
     1141 atttccacgg tatcttaaag gactcaaaga cagaccatat tcatgttata ctaagtatgt
     1201 acctcgcgat actgttgatt ctgttgaggt tgatgaaatg ggtatggatt caattaaagt
     1261 agttgttccg cagcctgtta taaatacatc aaaagcagaa gtaaagcaac ctgaaacaca
     1321 atcggtagaa gctgttgaaa ataagcgacc aagattattt caacgacgtg aaaaagaata
     1381 gtttttgttt aaaaatataa ttcatgaatt attcacaggt tttacctctt cgtaaacgtc
     1441 ataaattagg aggaatgaaa gaattgctta ttttagctct tcctattata gcctctcaat
     1501 catgcgaaac tattatggta tttaccgata gggtattttt atccaaatta agttctgagc
     1561 aaatgaatgc agctaccggt ggtggcattt catcattttt attattaact ttttttatag
     1621 gacttattgg ttttagtact gcacttaccg cacaattttt tggtgcaaaa aaatatgaac
     1681 aatgttcaat aactcttgta cagtctgtat ttatttcaat ttttgcgtat cctattataa
     1741 tttttgcttt gcctttgttg tattggtatt ttgaagttac tcatatatcg gcaggacaaa
     1801 tgatgtttca aaaacagtat gtacaaatat tagtttttgg ttctattatt tcacttatta
     1861 gacatagttt aagttgttat ttttccggta ttggcagaac taagattgta atgatggctg
     1921 ctatttttac aatggtagtg aatataggat ttaattatgt gtttatattt ggtcatttgg
     1981 gaatgcctgc tatgggaata aggggagcag cattgggaac tatattatcg gcattgtgtg
     2041 gagttgctgt tatgtcgtat gtgttttttt caaaaaaaaa tgttcaaaaa tttcaagtgt
     2101 ataaatcatt taaatttcat gcaaaaacgt ttaaaacatt attgcgatat ggtacaccag
     2161 caggtacaga attttttgtg aattttatag cgttttcatt acttgttttt ttatttcatt
     2221 catataatga acaaactgca acagctgcat caattatgtt taattgggat atggttacat
     2281 ttgttccatt agttggtata gaaataggag ttaccagtat ggtaggtaga tatgtgggtg
     2341 ctaaaaaatt acatgtagcc gaacgagcta catacacagc ggtaagatta ggttggtttt
     2401 tttctgcatt tgttgttctt atgtttgtgt ttattccaca cgtcttggtt
//
LOCUS       DAOTOW010000317         2444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33613_length_2444_cov_3.025115, whole genome shotgun sequence.
ACCESSION   DAOTOW010000317 DAOTOW010000000
VERSION     DAOTOW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33613_length_2444_cov_3.025115"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..>1140)
                     /locus_tag="P5243_06375"
     CDS             complement(339..>1140)
                     /locus_tag="P5243_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS19108.1"
                     /translation="MLIWTSFEAYNFLSPLCKPAHTNPFKPDWINVGFDTVSDVLFFY
                     TGAIFGILLYSFAPAYCVVALILFCITIILFAYWYPIKMYTQYAYFPFQRRLSQWNFP
                     ISQQNIERINQLISETTPKHILLLGKHNSGKTALAVSIGTEKTIQKNTCFYTTCIKFL
                     GLLETSDEDFCKKMNMPWSWRETEYLIIDDINSDVTDAALPEIISPEYISRIIAQSAF
                     KQRNIEVLKTTTIVWVVGKEEYKQEWINFIQSYGVSHNDIICIELAMC"
     assembly_gap    1141..1150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1151..1359)
                     /locus_tag="P5243_06380"
     CDS             complement(<1151..1359)
                     /locus_tag="P5243_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19109.1"
                     /translation="MKKKITFTTIIQQLKFDLIGKDSQRGITLTYGWLANQFGHFALG
                     FIPTQLFYLVYTHQVPVAHPLLWAPL"
     gene            complement(1377..2168)
                     /locus_tag="P5243_06385"
     CDS             complement(1377..2168)
                     /locus_tag="P5243_06385"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS19110.1"
                     /translation="MHTQNKTIKNQYEEIKKSIEYAKRLQHAVLPNKYRFSSVFKDHF
                     ILFKPKDVVSGDFYWIYEKKQKTYIVAADCTGHGVPGGFLSMLGISFLNQIIIRKRDI
                     RPHEIVNKLAILFAETIANYEDKISRDSMDISICLIHHDTNQLEYAGANNSLILIRDN
                     TLQELSVDKRSLHKEFLTEHFSFSGKCIDLLPHDKVYLFTDGFYDQFGEQNNKRISKR
                     LFKSLLLEHSNKPMAEQYEVFDTFFTQWKGTTEQIDDVLIIGFEL"
     gene            complement(2144..>2444)
                     /locus_tag="P5243_06390"
     CDS             complement(2144..>2444)
                     /locus_tag="P5243_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19111.1"
                     /translation="QYTKNKTLYNLIFLYSFACVIISVLIYILRNLGILGVSFITEHA
                     LKTGLALQATFISIATIIQFRDMLVETNKFLENMIGLRTKKYRNKMHYCTHKIKL"
BASE COUNT          821 a          369 c          371 g          873 t
ORIGIN      
        1 aaaaaccgtt atagtgtgaa tcacatttta tgagtatgta atcaaaaaat taatttcaat
       61 tcagaaaata gcttcaataa ttaagaaaag tttaaaaaaa ttgaagaaaa taaggttaaa
      121 cttaattatt tagtaaaaat ttgtttgctt tctattctta agattccttt taacaaagga
      181 atatgagtgt gtttctgtta aggcagtaat atatttgaca tataataaaa catttgtttt
      241 attacctttt tatatctttt tttcaatgat tatattgctg ctaaatatta catttgcaaa
      301 attaaacata tatacaaact atatgtatga atacttattc agcacattgc gagctctata
      361 caaataatat cattatgtga aacaccgtat gattgaataa aatttatcca ttcttgtttg
      421 tattcttctt tgcctactac ccatacaata gtagttgttt ttagcacttc aatatttcgt
      481 tgtttgaatg ccgattgtgc aattatgcga gaaatatatt caggcgaaat tatttcagga
      541 agagcagcat cggtaacatc gctatttata tcatctatta taagatattc tgtttctcgc
      601 cacgaccatg gcatattcat ttttttacaa aagtcttcgt ctgaagtttc taacaatcct
      661 aaaaatttaa tacatgtagt gtagaaacat gtattttttt gtattgtttt ttcagtacca
      721 attgataccg caagagctgt ttttcctgaa ttgtgtttac caagtaatag tatatgttta
      781 ggggtagttt ctgatatcaa ttgatttata cgttctatat tttgctgcga tattggaaaa
      841 ttccattgac tcaagcgtct ttgaaagggg aaataggcat attgcgtata cattttaata
      901 ggataccaat atgcaaacaa aataattgta atgcaaaata aaataagtgc aacaacacaa
      961 tatgccggtg caaatgaata aagcaatata ccaaatatgg caccggtata gaaaaataat
     1021 acatcactca cagtatcaaa tcctacattt atccagtcgg gtttaaacgg atttgtatgt
     1081 gctggtttac aaagcggtga aagaaagtta tacgcttcaa aacttgtcca tataagcatt
     1141 nnnnnnnnnn agaggtgccc acaacaatgg atgtgcaaca ggcacttgat gtgtgtatac
     1201 caaataaaac aattgtgtgg gaataaaacc taatgcaaaa tgaccaaatt gatttgcaag
     1261 ccacccatat gttaatgtta tacctcgttg tgagtctttg cctataaggt caaatttgag
     1321 ttgttgaata atagttgtaa aagtaatttt ctttttcatg ctattatcta atacagttat
     1381 aattcaaatc caataatcaa cacatcatct atttgttctg ttgttccttt ccattgagta
     1441 aaaaaagtat caaacacttc atattgctca gccattggtt tgttgctatg ctccaaaagc
     1501 aacgatttaa acagccgttt tgaaattcgt ttgttatttt gttcgccaaa ttggtcataa
     1561 aaaccatcgg taaacaaata cactttatca tgaggtaata aatcaataca ttttccggaa
     1621 aacgagaaat gttcggttaa aaattcttta tgcaatgatc gtttatcgac cgataattct
     1681 tgcaatgtat tatcgcgtat tagaataagc gaattatttg ctcctgcata ttctaattga
     1741 ttggtatcgt gatgtattaa acatatagat atatccatac tgtcgcgaga aattttgtct
     1801 tcgtagtttg ctatagtttc tgcaaataat attgcaagtt tgttcactat ttcatgcgga
     1861 cgaatatcgc gtttgcgtat tattatttga ttgagaaacg aaattcctaa catactcaaa
     1921 aaaccacccg gcacaccatg tccggtacaa tcggctgcaa ctatgtacgt tttttgtttt
     1981 ttttcgtaaa tccaataaaa atcgccactt acaacatctt tgggtttaaa cagaataaaa
     2041 tggtctttaa aaaccgacga aaatcgatat ttattcggta atacagcatg ttgtaatcgt
     2101 ttggcgtatt ctatactttt ttttatctct tcatattgat tttttatagt tttattttgt
     2161 gtgtgcaata atgcattttg ttccgatatt tttttgttcg caaaccaatc atgttttcta
     2221 aaaatttatt ggtttctaca agcatatcgc gaaactgaat aatagttgct atagaaataa
     2281 aggtagcctg cagtgccaat cctgttttaa gtgcatgttc ggtaataaat gaaacgccca
     2341 aaattccaag attacgaagt atataaataa gtacacttat aattacgcaa gcaaatgaat
     2401 atagaaaaat taaattatac agtgttttgt ttttagtgta ttga
//
LOCUS       DAOTOW010000318         2443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33633_length_2443_cov_2.685930, whole genome shotgun sequence.
ACCESSION   DAOTOW010000318 DAOTOW010000000
VERSION     DAOTOW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33633_length_2443_cov_2.685930"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="P5243_06395"
     CDS             complement(<1..397)
                     /locus_tag="P5243_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS19112.1"
                     /translation="MRQLKITKQVTNRDTPSLDKYLHEIGKVDLISAEEEVELAKRIR
                     EGDQKALDKLIKANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIVRLPLNKI"
     gene            607..>2443
                     /gene="rpsA"
                     /locus_tag="P5243_06400"
     CDS             607..>2443
                     /gene="rpsA"
                     /locus_tag="P5243_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRS19113.1"
                     /translation="MTKKEKKQNEEIENNDTLDVTNVEDVEQIEGEDFDSEDDEDLED
                     DEVIEEDSASDEEIDSIVTAAKAAQDDFDWEKFETNQNYYSSKERVNLEAMYDKTLST
                     VSENEVVEGVVVSVSKREVVVNIGYKSDGIVNVNEFRYNPELKPGDKVEVFVESQEDK
                     SGQLLLSHKKARALKSWDRVNQALEENEIVKGYVKCRTKGGMIVDVFGIEAFLPGSQI
                     DVKPIRDYDVYVGKTMEFKVVKINHEFKNVVVSHKALIEAELEVQKKEIISKLEKGQV
                     LEGIVKNITTYGVFIDLGGVDGLIHITDLSWGRITHPEEIVKLDEKINVVILDFDDEK
                     KRIALGLKQLTSHPWDTLDKDLQIGDKVKGKVVVMADYGAFVEIAPGIEGLIHVSEMS
                     WSQHLRSAQEFLNVGDEVEAVVLTLDREERKMSLGIKQLKADPWDSIDTKYPINSKHK
                     AIVRNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPAEFTSVGAEIEVVVLEVDKEN
                     RRLSLGHKQLEENPWDVFETIFNVDSIHEGTVVEVSEKNVVIALPYGVEAISIPSHIV
                     KEDGTQAKVDEKLDFKVIEFSKDAKKIFVSHTRTFEDAKKAQEDSE"
BASE COUNT          924 a          293 c          438 g          788 t
ORIGIN      
        1 caattttgtt tagtggtaat cttacaatac gtgcttgttc agctaatgct tgtaaaattg
       61 attgacgaat ccaccatact gcatacgaaa taaatttaaa gccacgagtt tcatcaaaac
      121 gttgtgctgc tttaattaat cctaaattac cttcatttat taaatcaggt aaacttaatc
      181 cttgattttg atattgttta gatacagata ctacgaaacg taagtttgct ttaataagtt
      241 tatctaatgc tttttgatca ccttcgcgaa tacgtttagc taattcaact tcttcttcag
      301 cagaaattaa atcaacttta ccaatttcat gtagatattt atctaacgat ggtgtatcac
      361 gatttgtaac ctgtttagtt attttaagtt gcctcatagt aaaaaatcct tttttatata
      421 tgtcaacatt tgtacgatat cctttcagaa atagtttgca aacttcgtaa ttttttcctt
      481 aatatttata tataaaccaa aaaataattg taaaaattta taaaatatta atagttaaat
      541 aaataaagtt ttatattttt gccgctccaa aaaatatata taatgtctaa ctattaatta
      601 tttaaaatga ctaaaaaaga aaaaaaacaa aacgaagaaa tcgaaaacaa tgacacttta
      661 gatgttacaa atgttgaaga tgtagaacaa attgaaggtg aagattttga ttctgaagat
      721 gatgaagatt tagaagatga tgaggttata gaagaagata gtgcatctga tgaagaaatt
      781 gattcaattg ttaccgcagc taaagctgca caagatgatt ttgattggga aaaatttgaa
      841 acaaatcaaa attattattc aagtaaagaa cgcgtaaatt tagaggcaat gtatgataaa
      901 acattgtcaa ctgtttcaga aaacgaagtg gttgaaggtg ttgttgtttc tgtatcaaaa
      961 cgcgaagtag ttgttaatat cggatataaa tcagatggta ttgtaaatgt taatgaattt
     1021 cgttacaatc ctgaattaaa accgggtgac aaagtagagg tatttgttga aagccaagaa
     1081 gataaatcag gacaattact attatcacat aaaaaagcac gtgctcttaa atcatgggat
     1141 cgtgtaaatc aagcgttaga agaaaacgaa attgtaaaag gatatgttaa atgtcgtact
     1201 aaaggtggta tgattgtaga tgtattcgga atagaagcat tcttacctgg ttcacaaatt
     1261 gatgtgaaac ctattcgtga ttacgatgtt tatgtaggaa aaacaatgga attcaaagtt
     1321 gttaaaatta atcatgaatt caaaaatgtt gttgtttctc acaaagcgct tattgaagca
     1381 gaacttgaag ttcagaaaaa agaaattata tcgaaacttg aaaaaggaca agtacttgaa
     1441 ggaatcgtta aaaatattac aacctatgga gtatttattg accttggtgg cgtagatgga
     1501 ttaattcata ttacagactt atcttgggga cgtattactc atcctgaaga aattgttaaa
     1561 ttagatgaga aaataaatgt tgtaatactt gattttgatg atgagaaaaa acgtattgca
     1621 ttaggtttaa aacaattaac atcacatcct tgggatactc ttgataaaga tttacaaatt
     1681 ggtgataaag taaaaggaaa agttgtagtt atggcagatt atggagcatt tgttgaaatt
     1741 gctcccggaa ttgaagggtt aattcacgtt tcagaaatgt catggtctca acacctacgt
     1801 agtgctcaag aattcttaaa tgtaggagat gaagttgaag ctgttgtttt aactttggat
     1861 agagaagagc gaaaaatgtc tttaggtatt aaacaattaa aagctgatcc ttgggactca
     1921 attgatacaa aatatccaat caacagtaaa cataaagcta tagtacgaaa ctttacaaat
     1981 tttggtgtgt ttgtagaaat tgaagaagga gttgatggtt taattcatat ttctgattta
     2041 tcatggacta aaaaaattaa acatcctgca gaatttactt ctgtaggtgc agaaattgaa
     2101 gtggtagtat tggaagttga caaagaaaat cgcagattaa gtttaggtca caaacaatta
     2161 gaagaaaatc cgtgggatgt gtttgaaact atatttaatg tagattcaat acatgaagga
     2221 actgttgtag aagtgagtga aaaaaatgta gtaattgctt taccatatgg cgtagaagct
     2281 atatctattc caagtcatat tgtaaaagaa gatggtactc aagcaaaagt tgatgaaaaa
     2341 ttagacttta aagtaataga attctctaaa gatgctaaaa agatttttgt ttcacatacc
     2401 agaacttttg aggatgcaaa aaaagcacaa gaagattctg aaa
//
LOCUS       DAOTOW010000319         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33712_length_2439_cov_3.685403, whole genome shotgun sequence.
ACCESSION   DAOTOW010000319 DAOTOW010000000
VERSION     DAOTOW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33712_length_2439_cov_3.685403"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="P5243_06405"
     CDS             <1..661
                     /locus_tag="P5243_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRS19114.1"
                     /translation="QLVNPKPIQIKGTIRVKKDSIIWVSITPALNIEAVRCVLTPDSV
                     RYINRIDKTYYEGGYDIIQKITAVPMNYSALQSVLLNEMIYYPFELNIDTLQVFQTYQ
                     IAKRKNELVAQNYSFRERRAIAGRRADTTAIFQSMNFMIDCFRVSEFAIVDDAKNLKV
                     QTKYSFFTLIDSIAFPKILTFEVKSHKKRMEFILNYEKVEFNIEQTYPFGVNAKYKQI
                     K"
     gene            710..2011
                     /locus_tag="P5243_06410"
     CDS             710..2011
                     /locus_tag="P5243_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRS19115.1"
                     /translation="MKKLLIIFFVCVCTGSVMAQKNIDKLKAEAEKAKKEIEITTRLL
                     KETEKNKNASVEKLNILNQQIQSRQKYLQSLQNEISDLERGIGSASAQIRTLNAQLEE
                     LQKEYNQTLFTLYKVRSSYDQFIFILSAENYNQAYNRLKYLQYYSDYMIKQAQRIEQL
                     QDSIQRRMIGMQELVNKKQTVVGIQKQETQKLSNDKHQENLIVQELTAKQQELKKKIE
                     EKRAQAKKLDNQIQEAIRAEIRAAEARAKAKAKAEAEAKAKADAEAAKLNKTSTTTTV
                     KPTTSAATPVVPTLTPEESIISKNFADNRGRLPWPTASGKITERFGTHPHPTLGFEVE
                     SNGIEITTNKGAKARAVFEGTVTKIVVIPGRNTAIIIKHGNYYSVYDNLINVQVKTGD
                     NVKVKDELGTVFTDPETGYTVLQLQIWKELQKLDPELWLSK"
     gene            2018..>2439
                     /locus_tag="P5243_06415"
     CDS             2018..>2439
                     /locus_tag="P5243_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19116.1"
                     /translation="MLQSNIHSIIDKLDDFIRKYYRMQCLKGLIIFLLLFISYSITVF
                     VIEYIAFLPVYTRTVLFYASLLLALSSLIWYVAIPLAKMYNIGKRISYIEASKIIQSH
                     FPEIKDTLLNALELSQLSNNDNNDLLIAAINQKSQILCP"
BASE COUNT          946 a          340 c          391 g          762 t
ORIGIN      
        1 acaacttgta aatcctaaac ctattcaaat aaaaggtact attcgtgtta aaaaagatag
       61 tattatttgg gtttctatta ctccggcact taatatagaa gcagttcgtt gtgttttaac
      121 acctgatagt gtaagatata ttaatagaat tgataaaaca tattatgagg gaggatatga
      181 tataattcaa aaaattacgg ctgtgcctat gaattattcg gcattgcaat cggtattatt
      241 aaatgaaatg atttattatc cttttgaatt aaatattgat acgttgcagg tatttcaaac
      301 ctatcaaata gcaaaacgaa aaaatgaatt agtagctcaa aattattctt ttagagaaag
      361 acgagctatt gcaggtcgta gagccgatac taccgctatt tttcaatcta tgaattttat
      421 gattgattgt tttagagtgt cggaatttgc aatagtagat gatgctaaaa atttaaaagt
      481 acaaacaaaa tattcgtttt ttactttaat tgattctatt gcttttccaa aaattcttac
      541 ttttgaagta aaatctcata aaaaacggat ggagtttata ttgaattatg aaaaagttga
      601 gttcaatata gaacaaacat atccgtttgg agtaaatgca aaatataaac aaataaaata
      661 gtcattcttt aagggtttta ctatttttga aacagtaaca aataaatcaa tgaagaagtt
      721 attaatcata ttttttgtct gtgtttgcac cggttcggtt atggcacaaa aaaatattga
      781 taaacttaaa gcagaagccg aaaaagcaaa aaaggagatt gaaattacta caagattatt
      841 aaaagaaact gaaaaaaata aaaatgcatc ggttgaaaag cttaatattc ttaatcaaca
      901 aattcaaagt agacaaaagt acttgcaatc attacaaaat gaaatttctg atctagaacg
      961 tggtattggt tcggcatcgg ctcaaattag aacactgaat gctcaattag aagagcttca
     1021 aaaagaatat aatcaaacat tatttacatt atataaagtt cgttcatcgt atgatcaatt
     1081 tatttttatt ttgtcagccg aaaattataa tcaggcatat aataggttaa aatatctgca
     1141 atattattct gattatatga taaaacaagc gcaacgaatt gaacaacttc aagatagtat
     1201 tcaacgtagg atgattggta tgcaagaact tgtaaataaa aagcaaacag tagttggtat
     1261 tcagaaacaa gaaacacaaa aattaagcaa tgataaacat caagaaaatt taatagttca
     1321 agaattaacg gcaaaacaac aagaattaaa gaaaaaaatt gaagaaaagc gagctcaagc
     1381 aaaaaaatta gataatcaaa ttcaagaagc tattcgtgca gaaatacgtg ctgcagaagc
     1441 acgtgctaaa gcaaaagcta aggccgaagc tgaggctaaa gcaaaagctg atgctgaagc
     1501 tgccaaatta aataaaacaa gtactacaac aactgtcaaa cctactacgt ctgcggctac
     1561 cccggttgtg cccactctta cacccgaaga gtcaattatt tctaaaaatt ttgctgataa
     1621 tagaggacga ttaccttggc caacagcaag tggtaaaatt acagaacgat tcggtactca
     1681 tccgcatcca accttaggtt ttgaagttga aagtaatgga atagaaatta caactaataa
     1741 aggtgcaaaa gcacgagcag tttttgaagg aacagttaca aaaattgtgg tcattcccgg
     1801 tagaaatact gctataatta taaaacatgg aaactattat agcgtttatg ataatttaat
     1861 aaacgtacag gtaaaaacag gtgataatgt aaaagtaaaa gatgaattgg gaaccgtatt
     1921 tactgatcct gaaaccggtt acacggtttt acaattacaa atttggaaag aattacaaaa
     1981 acttgatccg gaattatggc tttcaaaata aatgtatatg ttacaaagca atatacatag
     2041 tattattgat aaacttgacg attttattcg caagtattat cgtatgcaat gcctcaaagg
     2101 tcttataatt tttctgttac tttttatttc gtatagtatt accgtctttg ttatagaata
     2161 tatagcattt ttgccggtat atacgcgtac tgttttattt tatgcttctc ttttgttagc
     2221 actatcttca ttgatatggt atgtggcaat tccactggca aaaatgtata atattggtaa
     2281 acgaatttca tatatagagg cttctaaaat tatacaatct cattttcctg aaattaaaga
     2341 tacattactt aatgcgcttg aattatctca gttatcaaat aatgataata atgatttgct
     2401 tatagcggct ataaaccaaa aatctcaaat tttatgtcc
//
LOCUS       DAOTOW010000320         2437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33747_length_2437_cov_3.327036, whole genome shotgun sequence.
ACCESSION   DAOTOW010000320 DAOTOW010000000
VERSION     DAOTOW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33747_length_2437_cov_3.327036"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..2415
                     /locus_tag="P5243_06420"
     CDS             142..2415
                     /locus_tag="P5243_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS19117.1"
                     /translation="MLNEEIQIINSSPINSQLKSIPQSAEIFYNKGMECFNAKNYDIA
                     INYFEDALKEYPTYSNALNAMGAAWSWKQDFAKALLYYRQAFAIDSSNPVVFSNYIYA
                     LHSESLYDEISEVTLACPEQTFKQIQELDVFEYCIRALMNTEHYTKALEYANFVLEII
                     PNASVIKSLISEINSLHTINKPKVSKQPEASLEEILKDLENLVGLKQVKTDIENLMKY
                     IRIEKIRKERGIDIKPIALHAVFQGAPGTGKTTVARLLGAIYKQLGLLQKGHVVETDR
                     SGLVAEYVGQTAIKTNAVIDSALDGILFIDEAYSLYTGGSGDFGKEAIDTLLKRMEDN
                     RDRLVVVVAGYPHEMNMFIQSNPGLQSRFSRYFTFEDYSPSELLEICNRTVQASSMIL
                     LPDASQKLLKYFEYLHASKTKYFGNARIVRNLFEEIIQLQSARLADMKHISNTDLKTI
                     TKSDVDAAIGDDFKEEITETVDSILQEMNNLIGMNIVKQDIQRLLNFVKIEAIKKEKG
                     LQTQSIVLHTVFTGPPGTGKTTIARLLGRMYKSIGILPKGHVVEVSRADLVGEYVGQT
                     APKTNKVIDSALHGVLFIDEAYSLFSKNSSGSDFGSEVIDTLLKRMEDDRGLFVVILA
                     GYPDEMKMLIDSNPGLQSRFNRYITFSDYSAIELLQIFDVFCSKHGININKDAHNLLA
                     DFFSLAWQNKTKNFGNGRFVRNVYESLLQIQSNRIAILPEITENDLLTFTVDDVLQTI
                     ELFPIVKPQTYKSVGFR"
BASE COUNT          839 a          326 c          456 g          816 t
ORIGIN      
        1 gttagattta gatgatcaat atttttttta ttcaaccgat tatccacaac acgatattga
       61 agacaaaatt aatgaggggt attgtatctc acatgttgag tatgttaaat ctaagtggct
      121 tgttataatg gataaccaaa attgcttaac gaagagattc aaataataaa tagttcgcct
      181 attaattcac aattaaaatc aattccacaa tcagccgaaa tattttataa taaaggcatg
      241 gagtgtttta atgcaaaaaa ttatgatata gctattaatt attttgaaga tgcattaaaa
      301 gagtatccta catattcaaa tgctttaaat gcaatgggtg ctgcttggtc gtggaaacaa
      361 gattttgcaa aagctttatt gtattataga caggcatttg ctatagattc gagtaatccg
      421 gttgtgttta gtaattatat ttatgcatta catagtgaat ccttatatga tgaaatttct
      481 gaggttactc ttgcatgccc ggaacaaaca tttaagcaaa tacaagaact tgatgtgttt
      541 gaatattgta ttagggcact tatgaatacc gagcattata caaaagcttt agaatatgca
      601 aattttgttt tagaaattat tcctaatgct tccgtaatta agtctcttat atctgaaatt
      661 aattctttac atactatcaa taaaccaaaa gtttcgaaac aacccgaagc aagtcttgaa
      721 gagattctta aagacctaga aaatttggta ggattaaaac aagtaaaaac tgatatagag
      781 aatttaatga aatacattcg tatagaaaaa atacgtaaag aacggggtat agatattaaa
      841 cctattgcct tgcatgctgt atttcaagga gctccgggaa cggggaaaac taccgtagct
      901 cgattgcttg gagctatata taagcagttg gggcttttac aaaaagggca tgttgttgaa
      961 accgatcgtt caggattggt tgccgaatat gtaggtcaaa cagctattaa aactaatgcc
     1021 gtaattgata gcgctttaga tggtattttg tttattgacg aagcgtatag tttatatacc
     1081 ggtggttcag gagatttcgg gaaagaggct atagatacgt tgctaaaacg tatggaggac
     1141 aatagagata gattagtggt tgttgttgca ggataccctc atgaaatgaa tatgtttata
     1201 caatcaaatc ctggtttaca atcgcgtttt agtagatact ttacatttga agattattct
     1261 cctagtgaat tacttgaaat ttgtaataga acggtgcaag cttcctcaat gattcttttg
     1321 cctgatgctt ctcaaaaatt gctaaaatat ttcgagtatt tgcatgcttc taaaactaaa
     1381 tattttggaa atgctcgtat tgtaagaaat ttgttcgaag agattattca actacaatcg
     1441 gctcgattgg cagatatgaa acatataagt aatactgatt taaaaactat tacaaaatct
     1501 gatgtagatg ctgctattgg tgatgatttt aaagaagaaa ttactgaaac agttgattct
     1561 atattgcaag aaatgaataa tttaataggg atgaatattg taaaacaaga tattcaaaga
     1621 ctattgaatt ttgtgaaaat tgaagctatt aaaaaagaaa aaggattgca aactcaatct
     1681 atagtattgc atactgtatt taccgggcct ccgggtactg gaaaaactac tatagctcga
     1741 ttgttggggc gtatgtataa atctattgga attttaccaa aaggacatgt agttgaggtt
     1801 agtcgtgctg acctagtagg cgaatatgtg gggcaaactg ctcccaaaac caataaagta
     1861 atagattctg ctttgcatgg ggtattattt atagatgaag cgtattcgct tttttctaaa
     1921 aattcatcgg gaagcgattt cgggtcagaa gttatagata ccttacttaa acgtatggaa
     1981 gatgataggg ggttgtttgt cgtaattctt gcaggatatc cagacgaaat gaaaatgctt
     2041 atagattcga atcctggtct gcaatcacgt ttcaataggt atattacatt ttccgattat
     2101 tctgcaattg aattgttgca aatttttgat gtattttgtt caaaacatgg cataaatata
     2161 aataaagatg ctcataatct tcttgctgat tttttttctt tagcatggca gaataaaact
     2221 aaaaattttg gcaatggacg atttgtgcgt aatgtttatg aatccttatt gcaaatacaa
     2281 agtaatagaa ttgcgatatt acctgaaatt actgagaatg atttacttac ttttacagta
     2341 gatgatgttt tgcaaacaat agaattattt ccaattgtaa aacctcaaac atataaatct
     2401 gtggggtttc gctaaagctg taaatgtatt atatatt
//
LOCUS       DAOTOW010000321         2429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33901_length_2429_cov_2.643639, whole genome shotgun sequence.
ACCESSION   DAOTOW010000321 DAOTOW010000000
VERSION     DAOTOW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33901_length_2429_cov_2.643639"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="P5243_06425"
     CDS             complement(<1..418)
                     /locus_tag="P5243_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS19118.1"
                     /translation="MLKKILTICCIVCSIYAVAQTKKITGTVYTNTKEPLIAASVVEM
                     KAQYNAANDTGEQRVIGYTATTNGTFTLVDGTFELDVAQHQNAAIEISYIGYKTQIIP
                     ITEASTYAIVLQPDAIAIQTVVVTVPYSKQKKETYTG"
     gene            complement(452..1117)
                     /locus_tag="P5243_06430"
     CDS             complement(452..1117)
                     /locus_tag="P5243_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19119.1"
                     /translation="MTQQTLSPQDFLLATLDTILHYSKQQKQHKVEQILPIVHDIVRN
                     YPHILWHTPLIWKLAKVYMIMYHYDGGDDENFSMNLVKQAYIFSQRAVDLCEKQAEYT
                     ATDIHFDALHTQVMILNTCQDVLVGALAEVYMQLHSAESNIQSISNKLAQKIVPIIAY
                     NIVVKIDDAFTNFNNNAVIERMCNDFELSNPDITTAQLEQADKVHAILIHSFLRDCFP
                     NTK"
     gene            complement(1142..>2429)
                     /locus_tag="P5243_06435"
     CDS             complement(1142..>2429)
                     /locus_tag="P5243_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRS19120.1"
                     /translation="LKSLRELKQLKSGTVEHQDHADISVIGGVQTKRFKMVTVLFSSI
                     KGFNQLHETKNAQELIDELDTLYFEFDSIVERCNIKKIKSLGDTYMSAGGIPQKNRTN
                     PIEVVLAAMQIMDLLRRKKQENAARIWDISLGIHTGPVQAIISGKKNISYELKGDAAN
                     IASRIEAYSSAGKIIISDMTYEFVRDFFICDKIGGLPVKYTGVIDMYEVIGFKPKFSD
                     DDLLVIPNERFKLKLTIVRFADLEEYILNRLEKELPHYLYYHNVKHTMDVLIGVEVIG
                     VAEKVNEEELLLLKTAALFHDMGQIVQSKGHEEISCKYARDILPHYEYSQKHIDAVCE
                     LIMATQLPPNPKNLLQKIMCDSDLDYLGRTDFIPVSDTLYEELHYQNIISNKNDWNKM
                     QIAFISKHQYFTNFAKQNREVNKQNQIKRLKQLIVE"
BASE COUNT          770 a          414 c          347 g          898 t
ORIGIN      
        1 aaccggtata cgtttctttt ttctgtttgc tatacggtac cgttactact actgtttgta
       61 ttgcaatagc atcgggttgc aatactatag catatgttga tgcctcagtt ataggaataa
      121 tttgagtttt atagccaata taactaattt cgatagctgc attttggtgt tgtgcaacat
      181 ctaattcaaa cgtaccgtct acaagtgtaa atgtaccatt ggtagtggca gtatacccaa
      241 ttacgcgttg ttctccggtg tcgtttgctg cgttatattg agccttcatt tcaactactg
      301 atgcggcaat caatggttct tttgtatttg tatatactgt accagtaatt tttttagttt
      361 gtgctacggc atatatacta caaacaatac aacatatagt cagtattttt tttagcatgt
      421 tttaggtaaa aatttatgct aatatacaca ttcattttgt attaggaaaa caatcacgca
      481 aaaatgaatg tattaaaata gcatgtactt tatctgcttg ttcaagttga gccgtggtaa
      541 tatcgggatt gcttaattca aaatcattac acatacgctc aattacagca ttattattaa
      601 aatttgtaaa agcatcatct atttttacta ctatattata tgcaataatt ggtacaattt
      661 tttgagccaa tttatttgaa attgattgaa tattcgattc tgctgaatgt aattgcatat
      721 acacttcggc taaagcacct acaagtacat cttgacatgt atttagaatc ataacttgtg
      781 tatgcaatgc atcaaaatga atatcggtag cggtatattc agcttgtttt tcacataaat
      841 caacagctcg ttgcgaaaaa atatatgctt gttttactaa attcatacta aaattctcgt
      901 cgtcgccccc atcgtaatga tacataatca tataaacttt tgcaagtttc caaattaaag
      961 gggtatgcca taaaatatgg ggataattac gtacaatatc atgaacaata ggcaaaattt
     1021 gctctacttt atgttgtttt tgctgcttac tatagtgcaa aatagtatct aatgtagcca
     1081 ataaaaaatc ctgaggtgaa agcgtttgtt gtgtcatgta acaagtatta aaaaagaaaa
     1141 attattctac tataagctgt tttaatcgct taatttgatt ttgtttattt acttcacgat
     1201 tttgtttagc aaaatttgta aaatattgat gttttgatat aaacgcaatt tgcatcttgt
     1261 tccaatcatt tttgtttgaa attatatttt gataatgcaa ttcttcgtac aatgtatcag
     1321 aaacgggtat aaaatcggtt ctgcctaaat aatctaaatc agaatcacac ataatttttt
     1381 gtaacaaatt tttaggattt gggggtaatt gtgttgccat aataagttca cacacagcat
     1441 caatatgctt ttgagaatac tcataatgcg gcaatatatc acgtgcatat ttacaactaa
     1501 tttcttcgtg tccttttgat tgtactattt gccccatatc atggaacaat gcagctgttt
     1561 tgagcaataa taattcttct tcgttcactt tttcggcaac tcctataacc tcaacaccta
     1621 taagcacatc catagtatgt tttacattat gatagtataa ataatgcggt aattcttttt
     1681 ctaatctgtt gagtatatat tcttctaaat cagcaaacct aacaatagta agttttaatt
     1741 taaatcgctc attgggaatt accaataaat catcatcaga aaattttggt ttaaatccaa
     1801 tgacttcata catatcaatt actccggtat attttacagg taatccacca attttatcac
     1861 aaataaaaaa atcacgaaca aattcatagg tcatatccga tattataatt ttacctgccg
     1921 aactatatgc ttcaattcta cttgcaatat tagcagcatc tcctttaagt tcatatgaaa
     1981 tattcttttt accggaaatt atagcttgca ctggtccggt atgaattcct aaacttatat
     2041 cccaaatcct cgctgcattt tcttgttttt tacgtcgcaa caaatccata atttgcattg
     2101 cagccaatac aacttcaata ggattagttc tgtttttttg aggaattcca ccggcactca
     2161 tatatgtatc gcccagtgat tttatttttt ttatattgca tcgctcaact attgaatcaa
     2221 attcaaaata caatgtatca agctcatcta taagctcttg agcatttttg gtctcatgca
     2281 attgattaaa gcctttaata cttgaaaaaa gtacagtaac cattttaaac cgtttggtct
     2341 gtacaccgcc aattacagaa atatctgcat gatcttgatg ctcaactgta cctgatttta
     2401 attgtttaag ttctcgtaaa ctttttaac
//
LOCUS       DAOTOW010000322         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33958_length_2426_cov_2.814424, whole genome shotgun sequence.
ACCESSION   DAOTOW010000322 DAOTOW010000000
VERSION     DAOTOW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33958_length_2426_cov_2.814424"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="P5243_06440"
     CDS             complement(<1..796)
                     /locus_tag="P5243_06440"
                     /inference="COORDINATES: protein motif:HMM:NF025673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4380 domain-containing protein"
                     /protein_id="HRS19121.1"
                     /translation="MEQNTILYIKNAGVRVGIVPHLGGTIVFVSKNNSANILQSNDSL
                     WHNQIDVTAETDFYPLQGHTVWVGPQSHWWIQQTVNSKRKSEKAMWPPDPFITLGKYH
                     ILSQNEWSVTLQSPHSDVWGVTMQKEIAVNPDGSVFIQVTLINSSQDILAWDIWHNTR
                     MPGYSKAYVKTRKDHVRVVPVINDTSAEMPYTFVEDYFTYCPQAPKNTLSRSSKAFIY
                     PEMPVMYAFTPTHMLSISFEKHLAKEIHPEQGLVELYSHTEQDSAHA"
     gene            1017..1769
                     /locus_tag="P5243_06445"
     CDS             1017..1769
                     /locus_tag="P5243_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRS19122.1"
                     /translation="MFFIVSKILYFIITPIVWFFAAMVWAFFTKNSHTIKKIIGFTAL
                     ALYIFSNSFIVDECIRSYEIQTKHIDSLQCVYDYGIVLGGFNTFDAQFNRIQFNKASD
                     RLWQAILLYKKGKIKKIFISGGEGKIITEGYTESETTRDFLINIGIPPHDIVIENKSK
                     NTYQNAVYSAQLLKNHSCLLITSAIHMPRAYATFIKAGIKCEPFSTDRITGKRKFVFD
                     YCFIPNMQALENWRIFLRELIGYSIYKIMCYA"
     gene            complement(1815..>2426)
                     /locus_tag="P5243_06450"
     CDS             complement(1815..>2426)
                     /locus_tag="P5243_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19123.1"
                     /translation="YFFTFLFFGILIGFRLETNLKLLDMKKIGILSVIAIIVMQSCII
                     EAPAPEPAPYGRDGRDGRAFFKMNYGNFEPDYIETGGVIPSNFYWNTYYRTNPGFYTV
                     YYEYVENTRRGRIVYPYEIEVEVFVMAGEPGGYRYDGKDGDDVYFELILFPDGFEYYH
                     DIAYKSESTEKQQRTQIGYNESIVNNTKIVTTYYKLPPKNIEE"
BASE COUNT          803 a          413 c          354 g          856 t
ORIGIN      
        1 atgcatgagc actatcttgt tctgtatgcg aatataattc taccaatccc tgttcgggat
       61 gaatttcttt agccaaatgt ttttcaaaag aaatacttaa catatgtgtt ggcgtaaaag
      121 catacattac aggcatttcg ggatatataa aagcttttga gctgcgtgat aaagtgtttt
      181 tgggtgcttg agggcaatac gtaaaataat cttcaacaaa agtatagggc atttcggccg
      241 aggtatcatt aattaccggt accacccgca catggtcttt tcgagttttt acatatgctt
      301 tactataccc cggcattcga gtattatgcc atatatccca tgctaatatg tcttgcgaac
      361 tatttatgag tgtaacctga ataaatactg aaccatcagg atttaccgct atttcttttt
      421 gcatggttac cccccacacg tcactatgag gactctgtaa tgtaactgac cattcatttt
      481 gagacaatat atggtatttt ccaagggtaa taaacggatc aggcggccac atggcttttt
      541 ctgattttcg cttactattt actgtttgtt gtatccacca atgcgattga ggtccaaccc
      601 aaacagtgtg tccttgcaaa ggataaaaat ctgtttcggc agttacatct atttgattgt
      661 gccacaaaga atcattggat tgcagtatat ttgcactatt gttttttgat acaaaaacta
      721 tagttccacc caaatgaggc actataccaa ctctaactcc tgcatttttt atatatagta
      781 ttgtgttttg ttccattatt tttgtattaa tttgttgctt aaaaatagat aattcaactg
      841 tacttagtac acacacgctt acaagtaata ttaacaaaat ataccgaatg ttcatgtatg
      901 taaatataca aaatattata cacttattta gtatattaca catcattttt acttgaaatt
      961 tttatagtat tgtgtctttt ttttgtaata gataatgtta taaatgtata ctgaatatgt
     1021 tttttatagt atctaaaata ctctatttca ttattacacc tattgtgtgg ttttttgcag
     1081 ctatggtatg ggcttttttt acaaaaaact cacatacaat aaaaaaaatt ataggattta
     1141 cggcattagc tctgtatata ttttcaaatt cattcattgt tgatgaatgt attcgctctt
     1201 acgaaattca aacaaaacat attgattcgt tacaatgtgt atatgactat ggtatagttt
     1261 taggaggatt taatacattc gacgctcaat tcaatcgcat tcaatttaat aaagcaagcg
     1321 acagattgtg gcaagcaata ttgttatata aaaaaggaaa aattaaaaaa atttttattt
     1381 caggtggtga aggtaaaata ataactgaag gatataccga aagcgaaaca acacgagatt
     1441 ttttaataaa cattggtatt ccgccacacg atatagttat tgaaaataaa tcaaaaaaca
     1501 cataccaaaa tgctgtatat tcggctcaac tgttaaaaaa tcattcatgt ttacttatta
     1561 catcagccat acacatgcct cgagcttatg ccacatttat aaaagcaggt ataaaatgtg
     1621 aaccattttc aacagataga attaccggaa aaagaaaatt tgtgtttgat tattgtttta
     1681 ttcccaacat gcaagctctt gaaaattggc gaatattttt gcgagaactt ataggatatt
     1741 ctatatacaa aatcatgtgt tacgcataaa tagttattca aaaaaaaagc tgcaactatg
     1801 tgcagctttt ttgtttactc ttctatattc tttggcggca atttataata tgtcgtaaca
     1861 atttttgtat tatttacaat actctcatta tatcctattt gagttctttg ctgtttttct
     1921 gtactttctg atttatacgc tatatcatga taatattcaa atccatcggg aaacagaatt
     1981 aattcaaaat acacatcatc accgtctttt ccatcatatc ggtatcctcc cggttctccg
     2041 gccattacaa aaacttctac ttctatttca tacggataca ctatacgacc tcttcgcgta
     2101 ttttcaacat attcataata aacggtataa aatccgggat ttgttctata ataggtattc
     2161 caataaaaat tacttggaat tactccaccg gtttcaatgt aatcgggttc aaaattacca
     2221 taattcattt taaaaaaggc tctaccatca cgtccatctc taccatatgg tgcaggttcg
     2281 ggagcaggag cttcaattat acatgattgc atcactataa ttgctattac cgagagaata
     2341 cctatctttt tcatatctaa tagttttaaa ttagtttcta atctaaaacc aattaatatg
     2401 ccaaaaaata aaaaagtaaa aaaata
//
LOCUS       DAOTOW010000323         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_33975_length_2425_cov_2.955274, whole genome shotgun sequence.
ACCESSION   DAOTOW010000323 DAOTOW010000000
VERSION     DAOTOW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33975_length_2425_cov_2.955274"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..935)
                     /locus_tag="P5243_06455"
     CDS             complement(84..935)
                     /locus_tag="P5243_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS19124.1"
                     /translation="MNVYAIETGNFKLDGGAIFGVVPKTIWGAKYPADDKNLCNFCMR
                     CLLIEVDNRRILIDCGMGNTMPENLLKYYYINGDDTLLGSLLACGVEPEQITDVVLSH
                     LHFDHCGGAVTKHTDGSLHLTFPNAQYWVGKSQWESALKPNRRERPSFLKDNIEPLQH
                     SGKLNLIDSTTIIAPDVELRLYNGHTLGQIIPFIHYKNKTLVYTADFIPTSVHIPVSF
                     VCGYDIYPLQTMDETEAFLQEAVEKNYVLFFEHDIQVECCDLIQTPKGVAIGNVFTLE
                     EFKASVN"
     gene            1043..2419
                     /locus_tag="P5243_06460"
     CDS             1043..2419
                     /locus_tag="P5243_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRS19125.1"
                     /translation="MQRIHFIAIGGRAMHNLAIALQKNGYIVSGSDDEIYDPSYTNLK
                     NNNLLPSEIGWFPEKITTDIDTIILGRHAKNDNPELLRAQELGIRIYSYPEFIYSMSQ
                     HKQRVVIAGSHGKNTIASIVMHVLSYYQYEYDYLVSATVPGHEGTIHLTNTNKIIIIE
                     GDEHVTSTIDYRSKFEHYKPHIALLSGIAWDHVNTFPTFDMYVEQFKKYVNSIEHNGK
                     LIYNNDDSIVQQVINETPEHVQLVPYTMHPYEVFDNRSFLRISGGRLPVFIFGEHNMQ
                     NIAGAIKVCQFLKISEEKFYRAIKTFKGSGKHLQLIGKSKSVNIYLDFAHSPVKLNYT
                     IKAIREQFPNRELIVCLELYATSSLYDSFLNQYNGSMNLADEKIVYINPETLRKKGLE
                     SISAERIKEAFGSSKIKVFTSVEDVKSELYMIHWHNKTLLLMSSGNFSGLNYSEFAEK
                     IIGLSKEN"
BASE COUNT          870 a          436 c          383 g          736 t
ORIGIN      
        1 aatttttggt tttatatgta cctatttcat ctatgattta caaatgttca aaaaaatacc
       61 cgatcgagga atcttaaaaa aatctaatta acagatgctt taaactcctc taatgtgaat
      121 acattaccta ttgcaacacc tttgggggtt tgtattaaat cacaacactc tacttgaata
      181 tcgtgttcaa aaaacaatac atagtttttt tcaaccgctt cttgtaaaaa tgcttcggtt
      241 tcgtccatgg tctgcaaagg gtatatatca tatccacaca caaatgacac gggaatatga
      301 acagatgttg gaataaaatc ggcagtatat actaacgtct tatttttgta atgaataaaa
      361 ggtattattt gccccaaggt atgtccgtta tacaatcgca attcaacatc gggagcaatt
      421 atagttgtag aatctatcag attaagtttt ccactatgct gtaaaggttc aatattatcc
      481 tttaagaacg atggccgttc gcgtctgttt ggtttaagag ccgactccca ttgtgattta
      541 ccaacccaat attgagcatt cggaaatgta aggtgtagcg aaccatcggt atgtttagtt
      601 actgcacctc cacaatgatc aaaatgaaga tgtgaaagta caacatcggt aatttgttcg
      661 ggctcaacac cacatgcaag caatgagcct aataatgtat catcaccatt gatgtagtag
      721 tattttagta aattttcggg catagtatta cccataccac aatctataag tattctgcga
      781 ttgtctactt ctataagtaa acaacgcata caaaaattac ataaattttt atcgtcggcc
      841 ggatatttag ccccccatat agtcttagga accacaccaa atatagcccc cccgtcgagt
      901 ttaaaattac ctgtttctat agcatacaca ttcatatatt tctgtttaaa aatgttcctg
      961 attacataca aaaaatatca atacgcatac acatatctaa atttttgtat ttttattttc
     1021 aaatatacag caatttttta gtatgcaaag aattcatttt atagcaatag gcggacgagc
     1081 aatgcacaat ttggctattg ccttgcaaaa aaatggatat atagtatcgg gtagcgacga
     1141 tgaaatttac gacccgtcat ataccaactt aaaaaacaac aatttacttc catcagaaat
     1201 tggttggttt cccgaaaaaa ttaccacaga tatagataca ataattttag gccgacatgc
     1261 aaaaaatgat aatcccgaat tattacgtgc ccaagaatta gggatacgaa tttattcata
     1321 tccagagttt atatacagca tgtcgcaaca caaacaacga gttgtaatag ccggtagcca
     1381 tggtaaaaac accatagcat ctattgtaat gcatgtatta tcatattatc aatacgaata
     1441 cgattactta gtgtcggcta cggttcccgg ccacgaagga actattcact taactaacac
     1501 caacaaaatt ataataatag aaggtgatga acacgtaact tcaactattg attaccgttc
     1561 aaaatttgaa cactacaaac cacacattgc tttattgagc ggtatagcat gggaccatgt
     1621 caatacattt ccaacatttg atatgtatgt agagcaattc aaaaaatatg taaactctat
     1681 agaacataac gggaaattaa tctataataa tgacgacagc atagtacaac aagtaataaa
     1741 cgaaacgccc gaacatgtac aacttgtgcc atacactatg catccgtacg aagttttcga
     1801 caacagaagt ttcttacgaa tttcgggtgg cagattacct gtttttattt ttggggaaca
     1861 caatatgcaa aacatagcag gagctattaa agtttgccag tttcttaaaa tttccgaaga
     1921 aaaattttat cgtgccatca aaacattcaa aggttcgggt aaacacttac aattaattgg
     1981 caaaagtaaa tccgtaaata tctatttaga ttttgctcat tcacctgtaa aacttaatta
     2041 tactataaaa gccattcgag aacaattccc taaccgtgaa ctgattgtat gtttagaatt
     2101 gtatgccaca tcaagtttat acgactcgtt tcttaatcaa tataacggaa gcatgaattt
     2161 ggccgacgaa aaaatagtat atataaatcc cgaaacatta cgcaaaaaag gattagaaag
     2221 catttcagca gaacgaataa aagaagcatt tggcagttca aaaattaaag tttttacaag
     2281 tgttgaggat gtaaaatctg aattgtacat gatacattgg cataataaaa ccttattgct
     2341 catgagttca ggcaattttt cgggattaaa ctacagtgaa tttgccgaaa aaattattgg
     2401 attgagtaaa gaaaactaaa tatac
//
LOCUS       DAOTOW010000324         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34003_length_2423_cov_3.379645, whole genome shotgun sequence.
ACCESSION   DAOTOW010000324 DAOTOW010000000
VERSION     DAOTOW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34003_length_2423_cov_3.379645"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1244
                     /gene="purU"
                     /locus_tag="P5243_06465"
     CDS             372..1244
                     /gene="purU"
                     /locus_tag="P5243_06465"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRS19126.1"
                     /translation="MNTHNSANTATLLIHCKDQKGIVVSVTEFIHKNNGNILYLDQHV
                     DHQEAEFFMRVQWDLDGFAIPVEKIGDYFTTLIGDRYNMKFEIHLAKKVPRIAVFVSH
                     LPHCIYDILSRVRSGEWKVDIPVIISNHEKLRHVADEFGIPFHVFSITDKNKRQQEEA
                     IVELLQKEAIDTVILARYMQIISDWFISQYPNQIINIHHSFLPAFPGARPYHSAYERG
                     VKLIGATSHYVTPELDAGPIIAQGVTEVTHKDTVENMKRKGLDLEKIVLSKAIWSHIQ
                     HKTLVYNNKTIVFE"
     gene            1478..2026
                     /locus_tag="P5243_06470"
     CDS             1478..2026
                     /locus_tag="P5243_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRS19127.1"
                     /translation="MADTKAQFDEVKKICLDVFEKKYDDYGTAWRIMRPESVTDQIFI
                     KAQRIKSIGEKGVSKVAEGVVPEFIGIVNYGIIGLIQLELDASAPIELEKSHTLALYS
                     KYYEAAKELMTNKNHDYGEAWRSMRVSSYTDLILMKIHRTKQIEDNKGVTKISEGVDA
                     NYFDMINYAIFALIKLVIEKSE"
     gene            2029..>2423
                     /locus_tag="P5243_06475"
     CDS             2029..>2423
                     /locus_tag="P5243_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19128.1"
                     /translation="MKEKKWFLYGIGLSAVFAALYVTTQFSTFEDATWTKLFVLLSTI
                     LVGSVGLSFIFNTKQYTLNTARIFSGILFIFSGFVKAVDPLGSKYKFIDYFEAWNMNF
                     LDSWALTFGIILSTIEFVVGLTLLFKIYTR"
BASE COUNT          855 a          378 c          411 g          779 t
ORIGIN      
        1 ggttcgtcca ttttttatga tgtatgttgt ctttttaaaa ttacgcctaa catcccaata
       61 tcaccaattg gtgatatatt ccatgtatca atatcttatg tttgttcata aaaacatgca
      121 taatcaatac gttacagaaa tattaaattc attcggttca aaggtttttt atgtaacaat
      181 gttacgtttc aaatgtacat caaaatttat ataacacaaa acgttgaaaa ctttttttgt
      241 aatcaatgct ttttgtaagg cactatttgc atacaaaata attatacatg tatatatttt
      301 gagcagaata catctgcatc tatattctat tattttttca tatatttgct cgattttaca
      361 aaactatata tatgaatact cacaatagtg ctaatacagc aacattactt attcactgca
      421 aagaccaaaa aggtattgta gtatcggtta ccgaatttat tcacaaaaac aatggaaata
      481 ttctctatct cgatcaacat gttgaccacc aagaggcaga attttttatg cgtgtacagt
      541 gggatttaga tggatttgct attccggtag aaaaaatagg tgattatttt actacgctca
      601 ttggcgacag atacaatatg aaattcgaaa ttcatcttgc caaaaaagtt ccgcgtattg
      661 ctgtttttgt atcgcattta cctcattgta tttacgatat tttgtcgcgt gtgcgatcgg
      721 gcgagtggaa agttgatata cctgtaatta taagtaatca tgaaaaactt cgccatgttg
      781 ccgatgaatt tggcataccg tttcatgtat tttcaatcac cgacaaaaac aaacgccaac
      841 aagaagaagc tattgttgaa ttgttacaaa aagaagcaat agacactgta attttagctc
      901 gctatatgca aataatttcg gactggttta tttcgcaata tcccaatcaa attatcaata
      961 ttcaccattc gtttttaccg gcattccccg gtgctcgccc ataccattcg gcttacgaac
     1021 gtggagtaaa attaataggt gctaccagtc actatgttac tcccgaatta gatgccggac
     1081 ctattatagc acaaggagtt accgaggtta ctcacaaaga tacagtagaa aacatgaaac
     1141 gtaaaggttt ggatttggag aaaattgtac tttcaaaagc tatttggtca catattcaac
     1201 ataaaacctt ggtatataat aataaaacga ttgtgtttga ataaaaaaga ctgaaggagc
     1261 taaaaggtct gaaatcccaa agggatgata ttattataga aatcaaatat ttattactaa
     1321 ccacaccccc acaggggtga tattatggtt gtaaaaaagg tctaaataca cggaaagact
     1381 taaaagtctg aaaagttaaa aaaaaaagtg gtagaagtag tactgtaata gatagttcaa
     1441 ttcaaagttt ttgatatttt tgtaaaaaat aaaaacaatg gctgatacaa aagctcaatt
     1501 tgacgaagta aaaaaaatat gtttagatgt atttgaaaaa aaatacgacg actatggtac
     1561 tgcttggcgt ataatgcgtc cggaatcggt aaccgaccaa atatttataa aagcacaacg
     1621 aatcaaaagc attggcgaaa aaggcgtatc taaggttgcc gaaggtgttg tacccgaatt
     1681 tataggtatt gtaaattatg gtattattgg tcttattcag ttagaattag atgcatcggc
     1741 acctatagaa ttagaaaaat cacacactct ggcattgtat tcaaaatact acgaagctgc
     1801 caaagaattg atgaccaaca aaaatcacga ttacggcgaa gcatggcgaa gtatgcgcgt
     1861 aagttcatat accgatttaa ttcttatgaa aatacaccgc accaaacaaa tagaagacaa
     1921 taaaggtgtt acaaaaattt cggaaggtgt agatgctaat tattttgaca tgataaatta
     1981 tgcaattttt gcgttaataa aattagtcat tgaaaaatca gaataagtat gaaagaaaaa
     2041 aaatggtttt tatacggaat tggtttaagt gctgtatttg ctgcactcta tgtaaccaca
     2101 caattttcaa catttgaaga cgccacgtgg actaaactat ttgttctatt atcaacaatt
     2161 ttagtggggt cggtaggatt aagttttata ttcaacacca aacaatacac gttgaacacg
     2221 gcacgtattt tcagcggtat tttatttatt ttttcaggtt ttgtaaaagc ggttgaccca
     2281 cttggttcta aatataaatt catagattat tttgaagcat ggaacatgaa ttttcttgat
     2341 tcatgggcac ttacttttgg tatcatttta agtactatag aatttgttgt ggggcttact
     2401 ctattattca aaatatatac acg
//
LOCUS       DAOTOW010000325         2422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34018_length_2422_cov_3.384453, whole genome shotgun sequence.
ACCESSION   DAOTOW010000325 DAOTOW010000000
VERSION     DAOTOW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34018_length_2422_cov_3.384453"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /gene="panC"
                     /locus_tag="P5243_06480"
     CDS             <1..777
                     /gene="panC"
                     /locus_tag="P5243_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRS19129.1"
                     /translation="KSLGFVPTMGALHQGHISLIHQSVQTCDITCASIFVNPTQFNNP
                     HDLQTYPRTLDSDIAMLNEAGCDIVFVPEVIDMYPQGTQHIESYNFGSLERVMEGAFR
                     PGHFNGVAIIVKRLFDLIPADTAFFGLKDYQQVAIIRALVEQEKIPVRIVACPTVREP
                     DGLAMSSRNMRLNAEQRKQAVEISKTLFTVQALVGKKSVDDVKKFVIDTLNSVPLLSV
                     EYFEIVHQNTLQPIQNWNEPAVACVAVNVGDVRLIDNVLL"
     gene            789..1139
                     /locus_tag="P5243_06485"
     CDS             789..1139
                     /locus_tag="P5243_06485"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRS19130.1"
                     /translation="MVIEVLKSKIHRVTVTEANLDYIGSITLDENLMDAANLIANEKV
                     HVLNLMNGERIETYIIKGERGSGVVCLNGPAAHKFSPGHIVIVVSYALMDFEQAKSFV
                     PHIIFPDTATNTLV"
     gene            1142..2131
                     /locus_tag="P5243_06490"
     CDS             1142..2131
                     /locus_tag="P5243_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRS19131.1"
                     /translation="MKVSVVHIGKTILFSSLAFIFLFFAFKHVDFSSFWTDITQANYW
                     WVAIAIVCALIGYILRAVRWNLLLEPLGYTPSKINSFHAVVFGYFANLAFPRLGEVAR
                     CGVLNRKEQIPIDTLVGTVIIERIIDLVMLALLTIITFFIKIDFFGSFIYQHIVLPSS
                     EAVGRNYILIVSVLVTMTIVFFVLLRYSSKTNLIYQKIQSFCRGILHGIVSIKSVSNK
                     PLFVIYTVAIWAMYWLMSWLVCFSFEATSHFTAVDGLFLLIVGGIGMAVPVQGGIGVF
                     HFITAQAITMYGLSEHTGLLYATVNHESQLLFIIVMGCISSFYIFIAKNKQHV"
     gene            2128..>2422
                     /locus_tag="P5243_06495"
     CDS             2128..>2422
                     /locus_tag="P5243_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRS19132.1"
                     /translation="MSFLQHIQNKIVTQPQAVDIVSQWKQQGETVVFTNGCFDIIHKG
                     HVHYLALARDLGTKLIVGLNTDESVSALKGPNRPVKELESRALTLAAFACIDLI"
BASE COUNT          741 a          354 c          478 g          849 t
ORIGIN      
        1 aaatcattag ggtttgtgcc aactatggga gctttacatc aagggcacat ttcacttata
       61 catcaaagtg tacaaacatg tgatattacc tgtgcaagta tttttgtaaa tcctacgcaa
      121 tttaataatc ctcatgattt acaaacgtat cctcgaactc ttgattcaga tattgctatg
      181 cttaacgaag ctgggtgtga tattgtattt gtgcccgaag ttattgatat gtatccgcaa
      241 ggaactcagc acatagaatc atataatttt ggctcgttgg aaagggttat ggaaggagct
      301 ttcagacccg gtcattttaa tggtgtggca attatagtaa aacgattatt tgatttaata
      361 cctgccgata cggctttttt tggacttaaa gattaccaac aggttgccat tattcgtgct
      421 ttggtagaac aagaaaaaat tccggtacgc atagtagcat gcccaactgt tcgcgaaccc
      481 gatggtttag caatgagttc gcgtaatatg cgattaaatg ccgaacaacg taagcaggct
      541 gttgaaattt ccaaaacatt atttacagtt caggctttgg ttgggaaaaa atcggtagat
      601 gatgtaaaaa aatttgtaat tgatacacta aatagtgtac ctttactttc ggtagaatat
      661 tttgaaatag ttcatcaaaa tacattgcag cctatacaaa attggaatga accggcagta
      721 gcttgtgtgg ctgtaaatgt aggtgatgtg cgattaatag ataatgtgtt gttataatac
      781 tatacaaaat ggtaattgaa gttttaaaat caaaaattca cagagttacg gtaaccgaag
      841 ctaatttaga ctatattggc agcataactc ttgatgaaaa tttgatggat gcagcaaatc
      901 ttattgcaaa cgaaaaagtt catgtactca atcttatgaa tggagaacgc attgaaacat
      961 acataataaa aggagaacgt ggatcggggg tagtttgttt aaacggtcct gcagcacata
     1021 aattcagtcc cggtcatatt gttatagtgg tatcatatgc tcttatggat tttgaacaag
     1081 caaaatcatt tgtgcctcat attattttcc ccgatactgc cactaatact cttgtataat
     1141 tgtgaaagta tcggttgttc atattggtaa aactattctt ttttcatcgt tagcctttat
     1201 tttcttattt tttgctttta aacatgttga tttttccagt ttttggacag atataactca
     1261 agcaaattat tggtgggtag ctattgctat tgtgtgtgct ttaatagggt atatattgcg
     1321 tgctgtaaga tggaatttac ttttagaacc attaggatat actccttcaa aaataaatag
     1381 ttttcatgcc gtagtttttg gttattttgc caatttagca tttccgcgtt taggtgaggt
     1441 tgctcgatgt ggtgtactca atcgtaaaga acaaatacct attgatacgt tggttggtac
     1501 tgttattatt gagagaatta ttgatttggt tatgttagct ttgttaacaa taattacctt
     1561 ttttattaaa atagattttt tcgggtcatt tatatatcaa catattgtat tgccctcgtc
     1621 ggaggccgta ggcagaaatt atatacttat agttagtgta ttggtaacca tgactattgt
     1681 attttttgta ttactgcgct atagtagtaa aacaaatctt atatatcaaa aaatacaatc
     1741 attttgcaga ggaattttac atggtattgt ttctataaaa tcagtttcaa ataaaccatt
     1801 atttgtaata tatacagttg ccatatgggc tatgtattgg cttatgtcgt ggttagtatg
     1861 cttttcgttt gaagccactt cgcattttac cgctgttgat ggtttgtttc ttttaatagt
     1921 tggagggata ggtatggctg tgccggtgca gggtggtata ggagtgtttc attttataac
     1981 ggcacaagct attactatgt atgggctttc ggaacatacc ggattgttgt atgctacggt
     2041 aaatcatgaa tcgcaattat tatttattat tgttatgggt tgtatttcaa gtttttatat
     2101 ttttatagca aaaaacaaac agcacgtatg agttttttac aacatattca aaataaaata
     2161 gtaacacaac cacaagctgt tgacatagtt tcgcagtgga aacaacaagg tgaaacggta
     2221 gtgtttacta atggatgttt tgatatcatt cacaaaggac atgtgcatta tttagcgtta
     2281 gcacgtgatc tgggaacaaa acttattgtg gggttaaata ccgatgagtc tgtttcggca
     2341 ctcaaaggac caaaccgacc tgtaaaagaa ttggaatcaa gagcattaac gttagctgca
     2401 tttgcatgta ttgatttaat aa
//
LOCUS       DAOTOW010000326         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34191_length_2413_cov_2.733673, whole genome shotgun sequence.
ACCESSION   DAOTOW010000326 DAOTOW010000000
VERSION     DAOTOW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34191_length_2413_cov_2.733673"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..928
                     /locus_tag="P5243_06500"
     CDS             83..928
                     /locus_tag="P5243_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS19133.1"
                     /translation="MKSDSPIFEISPKTSLLAFNFKEIWQYRDLLVLFVRRDFVAMYK
                     QTILGPLWFFIQPIISSFVFYIIFNRVANIPTDGMPPYLFYLAGLTAWNYFSTCLNST
                     SNVFVGNAGIFGKVYFPRLITPLSIVISNLIKFAMQFVVFMMFYVYFYFQGAHIQFTM
                     YAWLFPILLVLMGGLGLAMGIIVSALTTKYRDLTFLVGFGVQLFMYATPVIYPLSQIP
                     EKYHVYIMLNPMTGVIETFRLAFLGVGIMNWTALWYSMATMIVLLFIGIILFNKTEKN
                     FMDTV"
     gene            939..2243
                     /gene="rseP"
                     /locus_tag="P5243_06505"
     CDS             939..2243
                     /gene="rseP"
                     /locus_tag="P5243_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRS19134.1"
                     /translation="MQTLQLIVSLSILVLIHELGHFTFAKLFGARVDKFYLFFNPWFS
                     IFKFKKGDTTYGLGWLPFGGYCKIAGMIDESMDKDQLKQAPQPWEYRSKTVWQRFFII
                     SGGVIFNFILALIIYSGMLYTWGETYLPNKNAHYGIYCDSLALSIGLQHGDKIIKVDN
                     TEPERFSDIHTLILLDNAKTITVDRKGIIETITIPKDFNLQIIAQQNPMFIVEFIPFF
                     IDSVIPGTPAYKAELKKNDRILAIDSIPTPTFIDFVKHIKKYANKQINLTILRNGYKQ
                     IIPLTVSPEGTIGAYRKELKDIYTFETRSFSILSSIPAGIHMGVSTLVFYIKQMKFLF
                     TKEGSQQVGSFLSMGKLYDTGWNWQVFWSLTALFSVILAFMNILPIPALDGGHLLFIL
                     YEMITGKKPSDTFLERAQMIGMTIVLTIFVYAIVMDIGRLFE"
BASE COUNT          801 a          365 c          359 g          888 t
ORIGIN      
        1 gtaatcaaat aaaattatgt tgtatgaaaa aaggcttaat cattaatctt tttcatactt
       61 ttgtatctga aaattataat gcatgaaatc tgattctcct atatttgaaa taagtcctaa
      121 aacttcattg cttgccttta attttaaaga aatatggcaa tatcgtgatt tacttgtttt
      181 atttgttaga cgagattttg ttgctatgta caaacaaact attttaggac cactttggtt
      241 ttttatacaa cctattattt catcgtttgt attttacatt atattcaata gagtagcaaa
      301 tatacctacc gatggaatgc caccctattt attttattta gcaggactta ctgcttggaa
      361 ttattttagc acatgcctta acagtacatc aaatgtgttt gtgggaaatg ccggcatttt
      421 tggtaaagtc tattttccaa ggcttattac accattatct atagttattt ccaaccttat
      481 aaaatttgcc atgcaatttg ttgtttttat gatgttctat gtatatttct attttcaagg
      541 agctcatatt caatttacaa tgtatgcatg gttatttcct attttattag tattaatggg
      601 tggattaggt ttagccatgg gaataatagt ttcggcactt accacaaaat accgtgattt
      661 aaccttttta gtaggatttg gtgttcaact atttatgtat gctacccctg ttatttatcc
      721 tctttcgcaa atccctgaaa aatatcatgt atatataatg cttaatccta tgacaggtgt
      781 aatagaaaca tttcgattag catttttggg tgttggtatc atgaattgga cagctttatg
      841 gtacagcatg gcaactatga tagtactatt atttattggt atcatactct tcaacaaaac
      901 cgagaaaaat tttatggata ccgtttaatt tcacatatat gcaaacactt caacttatag
      961 taagtctatc tatattagtt ctaattcatg aattaggaca ttttactttt gcaaaattat
     1021 ttggagctcg agttgacaaa ttttatttat ttttcaatcc ttggttttca atttttaaat
     1081 ttaaaaaagg tgatactact tatggattag gatggttacc atttggcgga tactgtaaaa
     1141 tagcaggcat gatagatgag tcaatggaca aagaccaact aaaacaagca cctcaacctt
     1201 gggaatacag gtcaaaaaca gtttggcaac gattttttat aatatccggt ggagttattt
     1261 ttaattttat acttgctctt attatttatt cgggtatgtt atatacttgg ggtgaaacat
     1321 atctgccaaa caaaaatgct cactatggta tttattgtga ttcgttggct ctttcaattg
     1381 gattacaaca tggtgacaaa attattaaag tagataatac cgaacccgaa cgtttttctg
     1441 atattcacac attgatactt ttagataatg ctaaaactat cactgtagac cgtaaaggta
     1501 taattgaaac tattactatt cctaaagact ttaatttaca aataatagca caacaaaatc
     1561 caatgttcat agtagaattt attccatttt ttattgattc tgttataccg gggactccgg
     1621 cctacaaagc agaattaaaa aaaaatgata gaatacttgc aatagacagt ataccaactc
     1681 ctacatttat agattttgta aaacacatta agaaatatgc caacaaacaa ataaacctta
     1741 ctatactaag aaacggatat aaacaaataa taccccttac tgtttcacct gaaggcacta
     1801 ttggagcata tcgtaaagaa ctcaaagata tttatacatt tgaaacacgt tctttttcga
     1861 ttctttcatc tattcccgcc ggaatacaca tgggagtatc aactcttgta ttttatatta
     1921 aacaaatgaa atttcttttt accaaagaag gatctcaaca agtgggcagt ttcctttcaa
     1981 tgggtaaatt atacgatacc ggttggaatt ggcaagtatt ctggagttta actgcattat
     2041 tttctgtaat tttagcattt atgaatatat tgccaatacc tgcattagat ggtggtcatt
     2101 tattatttat actgtatgaa atgataaccg gaaaaaaacc aagcgataca tttttagaac
     2161 gtgcacaaat gattggcatg actatagttc ttaccatttt tgtatatgct attgttatgg
     2221 atattggccg attatttgaa taaataaatg tatatgttga aaaaaattat ttcatatatc
     2281 tttaaaacaa taaaacaaag ctcatttgtt catagatatt tagaatctcc tatcaattta
     2341 aaagcattta ctacaatagg agtatttact gattcttttg gcaattcatt tgaaattcgc
     2401 aaaggattac gtt
//
LOCUS       DAOTOW010000327         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34296_length_2407_cov_3.434524, whole genome shotgun sequence.
ACCESSION   DAOTOW010000327 DAOTOW010000000
VERSION     DAOTOW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34296_length_2407_cov_3.434524"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /gene="yajC"
                     /locus_tag="P5243_06510"
     CDS             complement(<1..246)
                     /gene="yajC"
                     /locus_tag="P5243_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRS19135.1"
                     /translation="MNVGSIILQATASGQNPMGNLILLVGIMVIFFFFMVRPQMKRQK
                     EQRKFREGLQKGDSVVTLGGIYAKVVELKDNNIVIIEI"
     gene            complement(277..705)
                     /locus_tag="P5243_06515"
     CDS             complement(277..705)
                     /locus_tag="P5243_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS19136.1"
                     /translation="MKKFGFFVMVVLAGILSACNNSETKEDATELNDSVVKTTVVFDE
                     PKFDFGTITQGEVVEHTFFFTNTGSADYTIETVQASCGCTTPDYTQTPVKPGEKGKVV
                     VKFNSAGRSGNQSKTVTVYGNSEEGKIQLSFVAKIAEPTK"
     gene            complement(727..1668)
                     /locus_tag="P5243_06520"
     CDS             complement(727..1668)
                     /locus_tag="P5243_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRS19137.1"
                     /translation="MINRRLIRIKTLQTVYSYRKSDTKIFAEHYKEFNHSIEKSYELY
                     LLFLQLLPELQHYAHIRIDQIKNRIIKDDNEWQRLLPFANNLVIQQIQSNSQFQSLVK
                     KHTISWSHQQPLIKQLFKTIVQSSLYDTYIQSLSTYENDKKFVRKVLSSIILESEDIY
                     QFLEEYSIYWNDEIEYIISIVEKTIKQFKETVAGGGEILPKYKDEEIEHFSKTLFTKT
                     IEKWNEYNVYIDKKLENWEQERVAEIDVILLQIAITEAVEFCEIPLKATLNEYIELAK
                     WYSTEKSNMFVNGLLHTIFKELRQQKIIVKKGRGLLE"
     gene            complement(1825..>2407)
                     /locus_tag="P5243_06525"
     CDS             complement(1825..>2407)
                     /locus_tag="P5243_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HRS19138.1"
                     /translation="AWQLGQQSGNAIAQVLFGDYNPQGKLPMSFPRSVGQIPVYYNHL
                     NTGRPEGFNKVFWSHYMDIENTPLYSFGHGLSYTTFAYSNIVLKETKKGEYTITAHIT
                     NTGNRTGTETPQLYIRDEVASVVRPVKELKGFTKVTLSPGESKQIEFRLQRADLGFYT
                     ETGKLTVEPGDFEIMIGSSSSTILLNTKLTLTE"
BASE COUNT          709 a          407 c          323 g          968 t
ORIGIN      
        1 tatttctata attacaatat tattgtcttt taattctact actttagcat atatacctcc
       61 aagagtaact acagaatctc ctttttgtaa tccttctctg aattttcgtt gttctttttg
      121 acgtttcatt tgtggacgca ccataaagaa aaagaaaatt accataatac caaccaaaag
      181 gataagattt cccattggat tttgaccaga cgctgttgcc tgtaaaatta tacttcctac
      241 attcatgttt tttgtattta aaattattac tatttactat ttagttggtt ctgctatttt
      301 tgcaacaaaa gaaagttgta ttttaccttc ttctgaatta ccatatacag ttaccgtttt
      361 tgattgattt ccgcttctgc ctgctgaatt aaatttcaca acaactttac ctttttctcc
      421 cggttttaca ggtgtttgag tatagtcggg tgttgtacag ccgcatgatg cttgtactgt
      481 ttcaattgta taatctgctg accccgtgtt agtaaaaaag aatgtatgct ctacaacttc
      541 tccttgggta atggttccaa aatcaaattt tggctcatca aatacaactg ttgtttttac
      601 taccgaatca ttaagttctg ttgcatcttc ttttgtctcg ctattgttac aagcacttag
      661 aatacctgcc aaaactacca taacaaaaaa tccaaacttt ttcatattac tatattttaa
      721 aaattattat tctaataaac ctctaccttt tttaacaata attttttgtt gccgcaattc
      781 tttaaaaatt gtatgcaaca atccgttcac aaacatatta cttttttcgg tactatacca
      841 cttagcaagt tcaatatatt catttaatgt tgcctttagt ggtatttcgc aaaattctac
      901 tgcttcggtt atagctattt gcaacaatat cacatcaatt tcggcaactc gctcttgttc
      961 ccaattttct aattttttat caatatacac attgtattca ttccatttct ctattgtttt
     1021 ggtaaacaat gtctttgaaa aatgttctat ctcttcatct ttatattttg gtaaaatttc
     1081 tcctccgcct gcaactgttt ctttaaactg ttttattgtt ttttcaacta ttgatataat
     1141 gtattcaatt tcgtcgttcc aatagataga atattcttct aaaaattgat atatatcttc
     1201 tgattccaat ataatagacg aaagcacttt tcgaacgaat tttttatcat tttcgtatgt
     1261 tgagagtgat tgaatatatg tatcatacaa cgacgactgt acaattgttt taaacaattg
     1321 ttttataagc ggttgctgat gagaccatga aatagtatgt ttttttacta acgattgaaa
     1381 ttgagaattt gattgaattt gttgaatgac tagattgttt gcaaatggta ataatcgttg
     1441 ccactcattg tcatctttta taattctgtt ttttatttga tctattctaa tatgagcata
     1501 atgttgtaat tctggtaata attgcaaaaa caacaaatat aattcgtatg atttttctat
     1561 actatggtta aattctttgt agtgttctgc aaaaatcttt gtatcacttt tacgataaga
     1621 atatactgtc tgtaatgtct ttattcgtat taaacgtcta ttaatcattg taaaaaaact
     1681 ctatttttta gactgcaaat gtactattaa tttttaattg gttattatta atccacaatt
     1741 attgtcttat tttaatattg ttttatacaa taattaacga cagtgtaaat tgatttgtaa
     1801 actaaatttt ttcaaagtct attcttattc tgtaagtgtt agttttgtat tgagtaaaat
     1861 agtacttgaa ctagaaccta tcattatttc aaaatcaccg ggttctacag ttaatttacc
     1921 tgtttctgta taaaatccta aatcggcacg ttgcaatcta aattctattt gtttgctttc
     1981 accggggcta agcgtaactt tcgtaaaccc ttttaattct tttacgggac gaacaaccga
     2041 cgctacctca tcacgtatat acaactgtgg tgtttctgta cctgttctgt ttcctgtatt
     2101 agtaatatgt gctgttattg tatattctcc ctttttagtt tctttaagta caatatttga
     2161 ataagcaaag gttgtataac ttaatccgtg accaaatgaa tataaagggg tgttttctat
     2221 atccatatag tgcgaccaaa atactttatt aaatccttcg ggacgaccgg tatttaaatg
     2281 attataatat acaggtattt gtccaacact gcgaggaaac gacattggca atttgccttg
     2341 cggattataa tcaccaaaca gtacttgtgc tattgcattt ccgctttgtt gccccagttg
     2401 ccatgct
//
LOCUS       DAOTOW010000328         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34411_length_2401_cov_4.115942, whole genome shotgun sequence.
ACCESSION   DAOTOW010000328 DAOTOW010000000
VERSION     DAOTOW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34411_length_2401_cov_4.115942"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="P5243_06530"
     CDS             <1..745
                     /locus_tag="P5243_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LETM1 domain-containing protein"
                     /protein_id="HRS19139.1"
                     /translation="SHWLQNDYLYDKRNSIKKHILQIMIAASHCEGSIISTERLLVSY
                     FLASGNFDEKTELELQQQLKQGLYLNQIIIDTNLPYDIRLLLFENAVISVLSDGSINN
                     FEELFLGRLAEKLNISDNDVHYSIVMIQNFMLQNNKKLLYLHHKEGFEVITKSFAQRF
                     QVFFNKNSSKIITEISESRELVELLWKAKNEKLTDEEREKVKEQIIDLLRTIPSLTIF
                     MIPGGSILLPILLKILPEEILVPSSFRNK"
     gene            complement(760..1659)
                     /locus_tag="P5243_06535"
     CDS             complement(760..1659)
                     /locus_tag="P5243_06535"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRS19140.1"
                     /translation="MDTQQNSTIVEIDPKSGFCFGVINAIKRAEQALTENATLYCLGD
                     IVHNGAEVARLEEMGLQTITREQFYHLQNCTVLLRAHGEPPETYEIAKKNNITIIDAT
                     CPVVLQLQKKIKKGFEQQKQQNGQVLLFGKHGHAEVVGLQGQTNNEAIVVTHVHDIDK
                     IDVSKPIELFSQTTKSLSEFKELQQLIIAKKQKTGNMEVPFISHDTICRQVANRETEL
                     RKFASKYDLILFVSGKKSSNGKVLFDICKEVNPYTFFISSVHDIESVPYHTASSIGVC
                     GATSTPKWLMEDVARYIHEHTVH"
     gene            complement(1629..2300)
                     /gene="cmk"
                     /locus_tag="P5243_06540"
     CDS             complement(1629..2300)
                     /gene="cmk"
                     /locus_tag="P5243_06540"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRS19141.1"
                     /translation="MIIAIDGYSSCGKSTIAKGIAKELGFIYVDTGAMYRAVTLFCLQ
                     NNVIDNNEINEFLLKKYIDSIHISFVFDAETQSQQTYLNGVQVEKEIRGLEVASWVSP
                     VSKIGFVRQKLVGMQREFAEHGNIVMDGRDIGTVVFPNADYKFFITASAQVRAQRRYE
                     ELKAKGEQVDFEDVLHNIEQRDLIDTTRSESPLSQASDAIVFDTTNVTREEQLQWILN
                     KIQQS"
BASE COUNT          793 a          423 c          335 g          850 t
ORIGIN      
        1 ttctcactgg cttcaaaatg actatttata tgacaaacgc aacagtataa aaaaacatat
       61 acttcaaata atgatagcag cttctcactg tgaaggttct attatttcaa ccgaacgatt
      121 gttagtatca tattttttgg catcgggcaa ttttgatgaa aaaacagaat tggaactgca
      181 acaacaatta aaacaaggat tatatttaaa tcaaattata attgacacta acctacccta
      241 tgatattcga ttattacttt ttgaaaatgc tgtaatatct gttctaagcg atgggtctat
      301 taataatttt gaagaattat ttttaggtcg tttagcagaa aaacttaaca tttctgacaa
      361 cgatgtgcat tatagtatag taatgattca aaactttatg ttacaaaaca ataaaaaatt
      421 attgtattta catcataaag aaggatttga agtaattaca aaaagttttg ctcaacggtt
      481 tcaagtattt ttcaataaaa atagttcaaa aattattacc gaaatttctg aaagtcgtga
      541 acttgtagaa ttactatgga aagcaaaaaa tgaaaaatta accgatgaag aacgagaaaa
      601 agtaaaagaa caaattattg atttattacg aactattcca tcgctcacta tttttatgat
      661 tcccggaggt tctatattgt tgcctatttt attaaaaatt ttacctgaag aaattcttgt
      721 accaagttct tttagaaaca aataacgtga ttatttactc taatgtacgg tatgttcatg
      781 aatataccgc gccacatctt ccatgagcca cttaggtgta gacgtagctc cacaaactcc
      841 tatagaagaa gccgtatgat aaggaacact ttcaatatca tgaacacttg aaataaaaaa
      901 agtatacgga ttaacttctt tacaaatatc aaacaaaacc ttaccgtttg aacttttttt
      961 accacttaca aacaaaatta aatcatattt tgaagcaaat tttcgcaatt ctgtttctct
     1021 atttgcaacc tgtctacaga tagtatcatg agaaataaat ggaacttcca tattacctgt
     1081 tttttgcttt tttgcaatta ttaattgttg caattcttta aactcagaaa gactcttggt
     1141 cgtttgcgag aataattcta taggttttga aacatctatt ttatcaatat catgtacatg
     1201 ggttacaact atagcttcat tatttgtttg accttgcaaa ccaacaacct cagcatgacc
     1261 atgctttccg aataataaaa cttgaccatt ttgttgcttt tgctgttcaa atcctttttt
     1321 aatttttttt tgtagttgaa gtactacagg acatgtagca tctataattg taatattatt
     1381 tttttttgca atttcatatg tttcgggagg ttccccgtga gctcgtaata ataccgtaca
     1441 attttgtaaa tggtagaact gttcacgagt tatggtttgc aatcccattt cttcgagacg
     1501 agcaacttct gcaccgttat gaacaatatc acccaaacaa tacaacgttg cattttcagt
     1561 caaagcttgt tctgcacgtt ttattgcatt tattacccca aaacaaaacc ccgatttagg
     1621 gtcaatttct acgattgttg aattttgttg agtatccatt gcaattgttc ttctcgtgtt
     1681 acattcgtag tatcaaaaac tatagcatca gacgcttgtg ataaaggact ttcagatcgg
     1741 gtagtatcta ttaaatcacg ttgttctata ttatgtaaaa cgtcttcaaa atctacttgt
     1801 tcaccttttg ctttaagttc ttcatatctg cgttgagccc taacctgtgc cgatgctgtt
     1861 ataaaaaatt tataatcggc attaggaaat acaacagttc ctatatctct accatccatt
     1921 actatattgc catgttctgc aaattcgcgt tgcataccta ctaatttttg acgtacaaaa
     1981 ccaattttac ttaccggact tacccaactt gcaacttcta atcctcgtat ttccttttct
     2041 acttgcaccc catttaaata tgtttgttga ctctgtgttt cagcatcaaa aacaaatgaa
     2101 atatgaattg aatcaatata ctttttcaac aaaaattcat tgatttcatt attatcaatt
     2161 acattgtttt gcaaacagaa aagagttaca gccctataca tggctcctgt atcaacataa
     2221 ataaatccta attcttttgc aattcctttg gctatagtgc ttttaccgca cgatgaatac
     2281 ccgtcgatag ctattatcat aatgtgctca atttagatgc aattgtttgt atatttgttg
     2341 taagtgttaa aaaatgaaga cctccggcaa catgctgttt tgaccatcca taactgatgt
     2401 a
//
LOCUS       DAOTOW010000329         2401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34413_length_2401_cov_3.586530, whole genome shotgun sequence.
ACCESSION   DAOTOW010000329 DAOTOW010000000
VERSION     DAOTOW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34413_length_2401_cov_3.586530"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="P5243_06545"
     CDS             <1..408
                     /locus_tag="P5243_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS19142.1"
                     /translation="HAPHTIEEKTGVYTKAASGGPLVQHSLLAMLEFYKQGIISLPRI
                     IHKMCHAPADLFQIKKRGYIEKGFFADLVILNLSAQTKVTPQSLLYACNWSPFTNYTF
                     SSKIEKTIINGSIVYENGCVSDTLQAMALEFNR"
     gene            complement(410..>2401)
                     /locus_tag="P5243_06550"
     CDS             complement(410..>2401)
                     /locus_tag="P5243_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS19143.1"
                     /translation="MLRDSSIILYGTVGLLYIMFLAGLEVDFTDFKRNSGKSLVFGIF
                     TFVIPMTLGYLAGVYILHFGTLTSILLASMFASHTLIAYPIISRFGISQNKAVSIAIG
                     GTVITDTLALLVLAVIVGMTKGQINQEFWVRLTVSIILFASVILFIFPIIGRWFFKRF
                     SDKISQYIFVLAMVFLGAFLAQVAGIEAIIGAFLTGLSLNRLIPRTSALMNRIEFVGN
                     ALFIPIFLIGVGMLINYKAFLQDFETIKVGIVMTLTATLAKFLAALVTQKTFRLNADQ
                     RRIIFGLSNAQAAATLAAILVGYNIILNQDQIDAALAIGQVVEPIRLLNDSVLNGTIL
                     MILITCTHASFAAQKGARNIALSEAGSTDVENTKNDERILIPISDKSNIQELINVSVS
                     MKSKANKSGLYALTIVNTEKSAGAEKKAHALLDTAAIYASATDCQIQKLLRYDLTVSN
                     GIANIVKEQTITDVVLNVSYNNEHVHLGSTIDNLLTKCQANIIIYNALQPLNTIKRNF
                     IIIPQNAEKEVGFPYWIIKLWNLAKSTGVKNLFYGTKDTLQYIQEIYKKHHIDAEFFE
                     FDTFSDFLVLAREIKIDDTIVFILSRKDHPSYIQDMAKVPISLRQYFPQNNYLLFFPL
                     QSGVSDSTSPDYKNPSILEPLERLDDLGKTIANLFKINR"
BASE COUNT          848 a          411 c          340 g          802 t
ORIGIN      
        1 catgctccac atactataga agaaaaaaca ggtgtttata caaaagctgc ttcaggtggt
       61 cctttggtac agcattctct tcttgctatg ctcgaatttt ataaacaagg aattatttct
      121 ttacctcgta taattcataa aatgtgtcat gctccggcag atttattcca aataaaaaaa
      181 cgaggttata ttgaaaaagg attttttgca gatttagtaa tacttaatct ttctgctcaa
      241 acaaaagtaa caccgcaatc attattgtat gcatgtaatt ggtcaccatt taccaattat
      301 acattttcaa gtaaaattga aaaaacaata ataaatggct caatcgttta tgaaaacggc
      361 tgtgtaagcg atactcttca agctatggct cttgaattta accgataaat tatcggttaa
      421 ttttaaataa attagcaatt gtttttccta aatcatctaa acgttcaaga ggttccaata
      481 tagaaggatt tttataatca ggtgatgtac tatcacttac acccgattga aggggaaaaa
      541 ataatagata attattttgt ggaaaatatt gtcgtaatga aattggcact tttgccatgt
      601 cttgtatata tgacggatga tcttttctac ttaagataaa tacaatagtg tcatcaattt
      661 taatttcacg ggctaaaact aaaaaatcac taaatgtgtc aaattcaaaa aattctgcat
      721 ctatatgatg ttttttataa atttcttgaa tatattgtaa tgtatcttta gttccataaa
      781 acaaattttt aacacctgta ctttttgcta aattccataa tttaataatc caatagggaa
      841 atccaacttc tttttcggca ttttgaggta ttataataaa atttctttta atagtattaa
      901 gcggctgtaa agcattataa attattatat tagcttgaca ttttgtaagc aaattatcta
      961 ttgtagagcc taaatgaacg tgctcattgt tatatgacac attaagcacc acatcggtaa
     1021 ttgtttgttc ttttacaata tttgcaattc catttgatac agttaaatca taccgtaata
     1081 acttttgaat ctgacaatcg gtagccgatg cataaatagc tgccgtatca agaagtgcat
     1141 gagctttttt ctcggctccg gcagattttt cggtgttaac aatagtaaga gcatacaacc
     1201 ctgatttatt agctttagat ttcatgctca cacttacatt aattaattct tgtatatttg
     1261 atttatctga tattggaata agaattcttt catcgttttt tgtattttca acatctgttg
     1321 aacctgcttc ggaaagtgca atattacggg ctcctttttg tgctgcaaat gatgcatgtg
     1381 tgcatgtaat taaaatcata agaatggttc cgttcaacac actgtcattt aataatcgaa
     1441 taggctctac tacctgccct attgctaaag cagcatctat ttgatcttga ttaagaataa
     1501 tattatatcc cacaagtata gcagcaagtg tagcagcagc ttgagcatta ctcaacccaa
     1561 aaataattct tcgttgatct gcattaagtc taaatgtttt ttgggtaacc aatgctgcta
     1621 aaaattttgc taatgtggct gttagcgtca ttacaattcc tacttttatt gtttcaaaat
     1681 cttgcaaaaa tgccttataa ttaattagca ttcctacacc aataagaaaa attggaataa
     1741 acaaagcatt tcctacaaat tctattctat tcataagtgc cgatgtgcga ggaataagtc
     1801 tatttaacga taaccctgtt aaaaaagcac caattatagc ttcaattccg gcaacctgag
     1861 caagaaaggc gcctaaaaac accatagcca aaacaaaaat atattgagat attttatcag
     1921 aaaatcgttt aaaaaaccac cgaccaataa taggaaatat aaacaaaatc actgaagcaa
     1981 acaatataat agaaactgtt aatcgcaccc aaaattcctg atttatttga cctttagtca
     2041 taccaacaat tacggctaat actaagagtg caagcgtatc ggtaattaca gtacctccta
     2101 tagctatact tacagctttg ttttgcgaaa taccaaatct actaataata ggatatgcta
     2161 taagcgtatg agatgcaaac atactagcaa gcaatataga agtaagtgtt ccaaaatgta
     2221 atatatatac accggccaaa taccccaatg tcataggaat aacaaatgta aaaattccaa
     2281 acaccaaact ttttccacta tttcgtttaa aatcggtaaa atcaacttct aatccggcta
     2341 aaaacattat atataataat cctactgtgc catataaaat aatgctactg tcacgtaaca
     2401 t
//
LOCUS       DAOTOW010000330         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34598_length_2392_cov_2.919983, whole genome shotgun sequence.
ACCESSION   DAOTOW010000330 DAOTOW010000000
VERSION     DAOTOW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34598_length_2392_cov_2.919983"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="P5243_06555"
     CDS             <1..593
                     /locus_tag="P5243_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRS19144.1"
                     /translation="ESVDYLLTHNIIKPQQCIHADFLRISVPDIFSRSQYCIIGNFPY
                     NISSQIVFKIIEHREFIPGMTGMFQREVARRICSPKGSKEYGILSVLVQAFYTTEYHF
                     TLDEHDFIPPPKVKSGVISLYRKANYSLPCDEKLFVEVVKTGFNQRRKTLNNSLKKIL
                     GDAKLPVEIGLKRPEQLGVEDFIQLTKFIAQLPHKQ"
     gene            complement(598..846)
                     /locus_tag="P5243_06560"
     CDS             complement(598..846)
                     /locus_tag="P5243_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRS19145.1"
                     /translation="MIELIGYCAAFLTTLSFVPQALQVIKTKDTHSISLQMYVIFSAG
                     VVLWLVYGIWLRNIPMIIANSITIVLASIILYYKLKEK"
     gene            897..1127
                     /locus_tag="P5243_06565"
     CDS             897..1127
                     /locus_tag="P5243_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRS19146.1"
                     /translation="MHFEPHNLIDLVTTKMPYGKYQGTPIYKLPEFYLVWYHQKGYPQ
                     GKIGALLALMYEIQLNGLEYMLQPIISKYKTV"
     gene            1142..1618
                     /locus_tag="P5243_06570"
     CDS             1142..1618
                     /locus_tag="P5243_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRS19147.1"
                     /translation="MLKITLLVVGKTTDEYLKQGIQVYMERLRHFCNFSIIEIPELQV
                     TSKVSHTEIKKKEGDIIMSKIPRNARVFLLDEQGKQYASRQFATLVDNAMQTSVKELF
                     FVVGGAFGFSDEVYARYSSKISFSAMTFSHQMIRLFFVEQIYRAFSIINGLPYHHD"
     gene            1717..>2392
                     /locus_tag="P5243_06575"
     CDS             1717..>2392
                     /locus_tag="P5243_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRS19148.1"
                     /translation="MAEKQKRTRIVVAKKQDDSSYVPKKTGNTLERSNFTSKFEKKQG
                     LSSKSESSEYSAQSQNRNYVKQPIHTFSQKNSRKTLHGDEQFDAKQRKSKNSDFESKQ
                     RYQKQKTNSTFSQSHKPDVQSKSDNEPIRLNRFIANAGVCSRREADELIASGIISVNG
                     SIVTEMGYKVQRTDKVLMNGKELVSQKKVYILLNKPKDCITTKHDPEGRRTVFDYIQG
                     ACAENVE"
BASE COUNT          899 a          373 c          386 g          734 t
ORIGIN      
        1 atgaatcggt tgattattta ttgactcaca atataataaa accacaacaa tgtattcatg
       61 ctgatttttt gcgaatttca gtacccgata ttttttctcg ttctcaatat tgcataatag
      121 ggaattttcc ttataatatt tcaagtcaaa ttgtatttaa aattatagaa caccgtgaat
      181 ttattcccgg tatgaccgga atgtttcaac gtgaagttgc acgaagaata tgttcaccaa
      241 agggtagcaa agaatatgga atactatcgg tattggtgca agcattttat actaccgaat
      301 atcattttac gcttgatgaa catgatttta tacccccacc aaaggtaaaa tcgggtgtta
      361 tttcgttgta tagaaaagca aattatagtt tgccttgcga tgaaaaacta tttgtagaag
      421 tggtaaaaac cggatttaac caacgaagaa aaacattgaa taattcattg aagaaaatat
      481 taggcgatgc taaattacct gttgaaatag gacttaaacg tcccgaacaa ttaggagtag
      541 aggattttat tcaacttaca aagtttattg ctcaattgcc acataaacaa taatttacta
      601 cttctccttt aatttgtaat ataaaataat agatgcaagt acaattgtta tactgtttgc
      661 aataatcata ggaatattgc gcaaccaaat tccataaacc aaccacaata caactcctgc
      721 cgaaaaaatc acatacatct gcaatgaaat actgtgagta tcttttgttt ttattacttg
      781 caacgcttgt ggaacaaacg aaagtgtggt gagaaatgcc gcacaataac caattaattc
      841 tatcatatca tgtaaaaatg tttaccttta caaataaatt aaaaaaaatt ggacgaatgc
      901 attttgaacc tcacaattta atagatttag ttacaacaaa aatgccttat ggtaaatatc
      961 aaggtactcc aatatataaa ttacccgaat tttatttggt ttggtatcat caaaaagggt
     1021 atccgcaggg taaaattggt gcattgcttg cacttatgta cgaaattcaa ctcaatggat
     1081 tggaatacat gttacaaccc attatttcta aatacaaaac agtataatac tataaagcac
     1141 catgttaaaa ataacacttt tagtagttgg taaaacaacc gatgaatatt taaaacaagg
     1201 tatacaggtg tatatggaac gattacggca tttttgtaat ttttcgataa tagaaatccc
     1261 ggagttgcaa gtaacatcta aagtttcgca cactgaaatt aaaaagaaag aaggggatat
     1321 tataatgtca aaaattcctc gtaatgctcg agtttttttg ctcgatgaac aaggtaaaca
     1381 gtatgcatca cgtcagtttg caactttagt tgacaatgct atgcaaactt cggttaaaga
     1441 attgtttttt gttgttggcg gggcttttgg cttttcagat gaagtttatg caaggtattc
     1501 ttcaaaaatt tcattttctg ctatgacgtt ttcacatcaa atgattcgat tattttttgt
     1561 agaacaaata tatagagcgt tctcaattat aaacggttta ccgtatcatc atgattaata
     1621 attaaaaata ggagagattc aattgcgctg tttgcttttt aaattcttat aatagatgaa
     1681 taaaaattgt atctttgtca aaatttttcg atacatatgg cagaaaaaca aaaacgtaca
     1741 cgtatagttg tagcaaaaaa acaagacgac tcatcatacg tgccaaaaaa aacgggtaat
     1801 acgcttgagc gttccaattt tacaagtaaa tttgaaaaaa agcaaggact ttcatcaaaa
     1861 tcagagtcat ctgaatattc tgcgcaatcg caaaatcgta attatgtaaa acaaccaata
     1921 catacattct cacaaaaaaa tagtagaaaa acactgcatg gtgatgaaca atttgatgcc
     1981 aaacaaagaa aatcaaaaaa tagtgatttt gaatcaaaac aaaggtatca aaaacaaaaa
     2041 actaacagta ctttttctca atctcacaag cccgatgtac aatcaaaaag tgacaatgaa
     2101 ccaattcgtt taaacagatt tattgccaat gccggagtat gttctcgtcg cgaggccgat
     2161 gaattaattg caagcggaat tatatctgta aatggttcaa ttgttactga aatgggctat
     2221 aaagttcaaa gaactgataa ggttttgatg aatggaaaag aattggtttc gcaaaaaaaa
     2281 gtatatatat tacttaataa acccaaagat tgtataacaa ccaaacacga ccccgaaggt
     2341 agacgaaccg tgtttgatta tatacaaggt gcatgtgccg aaaatgtaga ac
//
LOCUS       DAOTOW010000331         2385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34740_length_2385_cov_3.181116, whole genome shotgun sequence.
ACCESSION   DAOTOW010000331 DAOTOW010000000
VERSION     DAOTOW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34740_length_2385_cov_3.181116"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5243_06580"
     CDS             <1..830
                     /locus_tag="P5243_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19149.1"
                     /translation="STLAMLDIIAHFEWKRPLYCMSNIDLSTILNLQNYMYSHGLASL
                     FTPIESLPSVESENQIHIEEMYNNLMKTYSWGNASANQGSVNEYERLIIQQYRSLFAI
                     AAQSCMQTKQFEKARNLVDACMQLFPQNVYNYDIFMIAIASIYYELGKVDMYESIVNA
                     LYSNIDNMLLYYRTVPKKFEKVLLHDVVQDVGFAIEMYQQALQIGDNKFSTKIAHMCT
                     LFIEDYFSFTRQISVCLPQEFQVNNMWIMQLQSNLAYVAYWYNYMKYNVVQEQKQQL"
     gene            839..1477
                     /locus_tag="P5243_06585"
     CDS             839..1477
                     /locus_tag="P5243_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS19150.1"
                     /translation="MKQPLLHINKPQPPLIIEKLYSRVFWRIPQEQKTVYLTFDDGPV
                     PIYTPWVLDLLDTYKVPATFFCVGENAQTYPDIFSEIQKRGHAIGSHTYNHLNGWKTA
                     NALYFENVCKAAQVVESKLFRPPYGKITLKQARVLSEKLTIIMWDVLSKDYDQTIDGE
                     TCFDNIKNYVSNGSIIVFHDSPKAFKNLEYALPKTIEWLQNEGYKCAKLHVD"
     gene            1480..>2385
                     /locus_tag="P5243_06590"
     CDS             1480..>2385
                     /locus_tag="P5243_06590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS19151.1"
                     /translation="MKLSIVIVNYNVKYILEQCLHSVFAAIQNIEAEVFVVDNNSVDG
                     SCTMITEKFPWVHLIENKENVGFSKANNQAIRISKGEYILLLNPDTVVEEDTFTKTIA
                     FMDEHPQGGGLGVAMIDGKGKFLPESKRGLPTLSVSFYKMFGFHKVFPHSKRFNYYYL
                     GHLSNNEVHEIEILSGAFMLMRKSVLDKVGLLDEEYFMYGEDIDLSYRIIKAGYKNYY
                     YPHTTIIHYKGESTKKASFKYVMVFYKAMEIFARKQLHMKKYSLYSLFIHIAIWARAL
                     IALVSRIIKKVLFPTIDTFVIYAGFF"
BASE COUNT          855 a          319 c          403 g          808 t
ORIGIN      
        1 aatctacatt ggctatgctc gatattatag ctcactttga atggaaacga ccattgtatt
       61 gtatgtcaaa tatagactta tcaaccattc ttaatcttca aaattatatg tattcacatg
      121 gtttagcatc gttgtttacc ccaattgaat cattaccatc ggtagagtcc gaaaatcaaa
      181 tacatataga agaaatgtat aataatttaa tgaaaacgta tagttggggt aatgcttcgg
      241 caaaccaagg aagtgttaat gaatatgaac gattaataat acaacaatat agaagtttat
      301 ttgctatagc agcacaatcg tgtatgcaaa ctaagcaatt tgaaaaagca cgtaatttgg
      361 ttgatgcttg tatgcaacta tttcctcaaa atgtatataa ttatgatatt tttatgattg
      421 caatagcttc tatatattat gaattaggta aagttgatat gtatgaatct attgtaaatg
      481 ccttatattc aaatatagat aatatgttgt tatactatag aacagtaccc aaaaaatttg
      541 aaaaagtatt gttacatgat gtagtacaag atgttggatt tgcaatagaa atgtatcaac
      601 aggcattaca aataggtgat aataagtttt caacaaaaat tgctcatatg tgtacattgt
      661 ttattgaaga ttatttttca tttactcgac aaatttcggt atgtttgcct caagaatttc
      721 aagtaaataa tatgtggata atgcaactgc aaagtaattt ggcatacgta gcgtattggt
      781 ataattatat gaaatacaat gtagtacaag agcaaaaaca acaattgtaa taatttagat
      841 gaagcaacca ttgttacata ttaataaacc gcaaccaccg cttattatag aaaagttata
      901 ttctcgcgtg ttttggcgta ttcctcaaga gcaaaaaacg gtatatctta cctttgacga
      961 tggtccggtt cctatttata ctccttgggt acttgattta ttagatacat ataaagtacc
     1021 tgctactttc ttttgtgttg gagaaaatgc acaaacgtat cctgatattt ttagtgaaat
     1081 tcagaaacgc ggtcatgcaa taggctctca tacatataac catttaaatg gttggaaaac
     1141 agcgaatgca ttatattttg aaaatgtatg caaagcagca caagttgtag aatcaaaatt
     1201 gtttagacca ccgtatggaa aaataactct taaacaagct cgtgttctat cagaaaaact
     1261 taccataata atgtgggatg ttttatcgaa agactatgat caaaccattg atggagaaac
     1321 ttgttttgac aatataaaaa attatgtaag caatggttca attatagttt ttcatgactc
     1381 acctaaggct tttaaaaatc ttgaatatgc attgcctaaa actattgaat ggctgcagaa
     1441 tgaaggatat aaatgtgcta aattacatgt ggattaatta tgaaactgtc tattgtaata
     1501 gtaaattata atgtaaaata tatactcgaa caatgcttac attcggtatt tgctgcaatt
     1561 caaaatattg aagctgaagt gtttgtggtt gacaacaatt ctgttgacgg ttcgtgtaca
     1621 atgattacag aaaaattccc atgggtgcat ttaattgaaa ataaagaaaa tgttggattt
     1681 tcaaaagcaa ataatcaagc tattcgtatt tcaaaaggag aatatattct tttacttaat
     1741 cccgatactg ttgtagaaga agatacgttt accaaaacaa ttgcatttat ggatgaacat
     1801 ccgcagggtg gagggttagg tgttgcaatg attgatggta aaggaaaatt tttacctgaa
     1861 tctaaacgtg ggttacctac tttgtcagtt tctttttata aaatgtttgg attccataaa
     1921 gtatttcctc actctaaacg gttcaattat tattatttag ggcatttatc aaacaatgaa
     1981 gtgcatgaaa ttgaaatact ttcaggagcg tttatgttaa tgcgaaaatc tgtacttgac
     2041 aaggttggat tgcttgatga agaatatttt atgtatggag aagatattga tttgtcatat
     2101 agaataataa aagcaggata taagaattat tattatccgc acaccactat aattcattac
     2161 aaaggagaaa gtacaaaaaa ggcaagtttt aaatatgtaa tggtttttta caaagcaatg
     2221 gagatttttg ctcgtaagca attacatatg aaaaaatata gcttatattc attatttatt
     2281 catatagcta tatgggcacg agctcttatt gctcttgttt cacgaattat taaaaaagtg
     2341 ctgttcccaa ctatagatac atttgtaata tatgccggat ttttt
//
LOCUS       DAOTOW010000332         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_34848_length_2379_cov_2.962995, whole genome shotgun sequence.
ACCESSION   DAOTOW010000332 DAOTOW010000000
VERSION     DAOTOW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34848_length_2379_cov_2.962995"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="P5243_06595"
     CDS             <1..432
                     /locus_tag="P5243_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19152.1"
                     /translation="EPISIEAPINITEPIIHIQNKQKQVDFIPHISQITQSSIIRLNP
                     MQGIQQAGQYSVINNEKVIANIAYNYSRKESQSDFYTVQDLEQIIESRQLQNTHIIPL
                     ETKTISETVNDVRLGTLLWRWFIIFAIMFACTEVLIIRYFK"
     gene            429..2156
                     /gene="tilS"
                     /locus_tag="P5243_06600"
     CDS             429..2156
                     /gene="tilS"
                     /locus_tag="P5243_06600"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRS19153.1"
                     /translation="MIIQKKHINDSLYYECSTLKNDYSNISYKLYKDYKIIIDCIIEF
                     WKDTTLENVEEFLQYLEEDFAKQELDFYTARFILNELMCEAPYSKSFHILNEPEETNA
                     NDIFCKNFEKAILETIEKHSLFTKEHDKLLMALSGGIDSTTLFYVLKSLGYNIEIAHC
                     NFNLRGQESKFDELSVKKIAKYTNTPYFIKRFNTTKYAEKHRISIEMAARELRYAWFQ
                     ELCNKHGFTKIVVAHNQNDTIETFFINLLRGSGIKGLQGIPITNGNIVRPMLTITRSD
                     IEKYCKIQLISYNVDITNLSNKYTRNKIRNIVLPVFQQISPDALSSINTSLKHIQQSY
                     AFYKEAIDKEIKEIVTYTPQGCKIEEKLLINHPHRDIITFEILQQFGFKSDVIQQIVA
                     CFGTQSGKKFESPTHRAIHDRNILYVKKIQKHSDFQKSIDVWKDQTIQTPIGILESKI
                     YASLVTRIVKNNSLVLQFDVDKLGKEITIRTWKQGDKIIPFGMKGSKKISDYLIDAKV
                     PIHEKEKILVIESNGTIVGIIGHTIHNFYAVTSETEMVYNLITIKDNVDTKKLITHPD
                     IPNNLFNLS"
     gene            complement(2197..>2379)
                     /locus_tag="P5243_06605"
     CDS             complement(2197..>2379)
                     /locus_tag="P5243_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19154.1"
                     /translation="IFSRIKSPSHPKVIARTIMKAISTQNPKTRYVAGSGAKPLLFLR
                     SIISDKCYDRIMRKMV"
BASE COUNT          963 a          357 c          305 g          754 t
ORIGIN      
        1 gaacctattt caatagaagc tcctataaac attaccgaac ctataataca cattcaaaac
       61 aaacaaaaac aagttgactt tattcctcat atatcacaaa ttacacaaag ttctataatt
      121 cgtttaaatc ctatgcaagg catacaacaa gcaggacaat atagtgtaat taacaatgaa
      181 aaagttattg ctaacattgc atataattat tcccgaaaag aatcacaatc ggatttttat
      241 acagttcaag atttagaaca aattatagaa tcacgtcaat tacaaaatac acatataatt
      301 ccattagaaa caaaaactat atcagaaact gtaaacgatg taagattagg aactcttttg
      361 tggcgctggt ttattatatt tgcaataatg tttgcttgta ctgaagttct tataattcga
      421 tatttcaaat gattattcaa aaaaaacata ttaatgactc tctatactac gaatgttcta
      481 cattaaaaaa tgattactcc aacatttcat ataaacttta taaagactac aaaattataa
      541 ttgattgtat tatagaattt tggaaagaca ctacacttga aaacgtagaa gaatttttac
      601 aatatcttga agaagatttt gcaaaacaag aactagattt ttatacagca cgtttcattt
      661 taaatgaatt aatgtgtgaa gctccttact ctaaatcttt tcatatttta aatgaacctg
      721 aagaaactaa tgcaaatgat attttttgta agaattttga aaaagctatt ttagaaacta
      781 ttgaaaaaca ttcattattt acaaaagaac atgataaatt actaatggca ttaagcggcg
      841 gtattgattc aactacttta ttttatgtat taaaatcact tggatacaat attgaaattg
      901 cacattgcaa ttttaattta cgaggacagg aatctaaatt tgatgaattg tcggtaaaaa
      961 aaatagctaa atatactaat accccttact ttataaaaag atttaataca accaagtatg
     1021 cagaaaagca tcgtatttca attgaaatgg ctgctcgtga acttcggtat gcttggtttc
     1081 aagaattatg taataaacac ggttttacta aaattgttgt agctcacaat caaaatgata
     1141 caattgaaac tttttttata aatctactga gaggttccgg aattaaggga ttacaaggta
     1201 ttcccattac aaatggaaac attgttcgtc ctatgcttac aattactcga tctgacattg
     1261 aaaaatattg caaaatacaa cttatcagtt ataatgtaga cataacaaat cttagtaata
     1321 aatatactcg aaataaaata agaaatatag tattacctgt ttttcaacaa atttcacccg
     1381 atgcattatc ttctatcaac acttcactca aacacataca acaaagttac gcattctata
     1441 aagaagctat tgacaaagaa attaaagaaa ttgtaacata tacaccacaa ggttgtaaaa
     1501 ttgaagaaaa actattaata aaccatcccc atcgcgatat aataacattt gaaatattac
     1561 aacaatttgg atttaaatca gatgttattc aacaaattgt tgcttgtttt ggaacccaat
     1621 caggtaaaaa atttgaatcg ccaacacata gagctataca tgatagaaat atattgtatg
     1681 taaagaaaat acaaaaacat tctgattttc aaaaatcaat tgatgtttgg aaagaccaaa
     1741 caatacaaac cccaatagga atacttgaat caaaaattta cgcctcactt gttaccagaa
     1801 ttgtaaagaa caactctctt gttcttcaat ttgatgttga caaattagga aaagaaatca
     1861 ctattagaac atggaaacaa ggagataaaa ttataccctt tggtatgaaa ggctcaaaaa
     1921 aaataagcga ttatttaatt gatgctaaag taccaattca tgaaaaagaa aaaatactgg
     1981 ttattgaatc aaacggtact atagtaggta ttataggaca cactattcat aatttttatg
     2041 ctgttacttc tgaaacagaa atggtatata atttaattac aataaaagac aacgtagata
     2101 caaagaaatt gataactcac ccggatatac caaataactt atttaatcta tcgtaatcag
     2161 aaaaaaggaa caagtacaca aattgtatta aaacacttat accatttttc tcattattct
     2221 atcatagcat ttatcactta ttatacttct taaaaacagt aagggcttag ctccggaacc
     2281 tgcaacatag cgagttttag gattttgtgt tgatatagct ttcataattg tacgagcaat
     2341 tacttttgga tgcgaaggac ttttaatacg actaaaaat
//
LOCUS       DAOTOW010000333         2369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35047_length_2369_cov_2.560069, whole genome shotgun sequence.
ACCESSION   DAOTOW010000333 DAOTOW010000000
VERSION     DAOTOW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35047_length_2369_cov_2.560069"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="P5243_06610"
     CDS             complement(<1..741)
                     /locus_tag="P5243_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19155.1"
                     /translation="MKNKTIVSILLLFAITLVACKKNNIEKNLHFPEITFLDKSTSLK
                     SEVTSNVDALYFGGLSIVAYHYRPGQAIQNRVGDAAYYEGKPLNWSYFLFVSPGYEEA
                     RSVLLNKSGEINIADYLHVSYNNAVWDFVDKKYVEQVDTFKVDMFEVRLDGSAVLKNN
                     KFYGCSPCMVADSANFRKKYPTYSNRNKYMAYTYWKGIDTISGSISIMFVNDAYISTP
                     LQVKVRNYKNTGETGLLDSYVIDKTTRTI"
     gene            complement(797..1690)
                     /locus_tag="P5243_06615"
     CDS             complement(797..1690)
                     /locus_tag="P5243_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS19156.1"
                     /translation="MENHIIEIDIEKMMHTAEGPVPLSVQTSIKAHELTALFGQSGAG
                     KTTLLRILAGLLQPDKGSIVFDNTIWFDSAKKIQIPPQQRNISYMFQDYALFPNMTVE
                     QNLLFAQNTKDVQKVTEILTVFGLLEFKNRKPAGLSGGQKQRVALARALARNPQLLLL
                     DEPLSALDSVMRLQLQNEIRKVQKLYNITTIIVSHDIHEIYKLASHVVVLEHGTITKI
                     GSPHEIFSNTAISSKVQITGHIAHIEQQDTLLSIVTVITGTNQIIKVLAFENDMKELY
                     IGSPVMVYTKAFNPMIKKINI"
     gene            complement(1675..>2369)
                     /gene="modB"
                     /locus_tag="P5243_06620"
     CDS             complement(1675..>2369)
                     /gene="modB"
                     /locus_tag="P5243_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRS19157.1"
                     /translation="TIKTMEHIFIQTLVLTAELAVCTTCIAIGIGLPLAYWLTYSKFK
                     LKPLFEALISMPMVLPPSVLGYYMLVAYSPNHWFGGWLFNEFGIRLAFSFEGILIASV
                     LFSLPFMVQPLQNGLRSLPDSIREASYTLGKSKTTTFFRVLLPNIKPSIITAVALTFA
                     HTIGEFGVVLMVGGNLPGQTRVASIAIYDEVQALNFDTANQYAIILFMLSLILLTTIY
                     SVNKKHDTWKII"
BASE COUNT          728 a          411 c          400 g          830 t
ORIGIN      
        1 aatggttcgt gtggttttat caatcacata tgaatctaac aagcctgttt cacccgtatt
       61 tttataattt cttactttta cttgcagagg ggtactaata taggcatcgt ttacaaacat
      121 aatagaaata ctgccactta tagtgtcaat gcctttccaa taggtatatg ccatatattt
      181 attacgatta ctatatgttg gatatttttt tctaaaatta gcactatcag ctaccataca
      241 tggcgaacat ccataaaatt tattgttttt aagaaccgct gaaccatcta atcgcacctc
      301 aaacatatca actttaaatg tatctacttg ttctacatat tttttatcca caaaatccca
      361 tactgcattg ttgtatgata catgaagata gtcggctata tttatttcgc cacttttgtt
      421 tagcaatacg cttcgagctt cttcataacc cggcgaaaca aataaaaaat aactccaatt
      481 taaaggtttt ccttcataat atgcagcatc acctactctg ttttgtatgg cttgtccggg
      541 acgatagtga tatgctacga tacttaatcc cccaaaatac agtgcatcta cattacttgt
      601 aacttctgat tttaacgatg tagatttgtc taaaaacgta atttcaggaa aatgtagatt
      661 cttctctata ttgttttttt tgcacgctac caatgttatt gcaaaaagca acaatatgct
      721 tacaattgtt ttgtttttca tatctgtttt gttttaattt atactttttc aaatatattg
      781 tattttattt tgtaagtcaa atatttattt tcttaatcat aggattaaat gcttttgtat
      841 aaaccattac cggtgaacct atatataatt ctttcatatc gttttcaaat gcaagtactt
      901 ttatgatttg gttggtaccg gtaataacag taactattga caaaagagtg tcttgttgtt
      961 caatatgagc tatatgtccg gtaatttgta ctttgctgct aatagcagtg ttgctgaaaa
     1021 tttcatgcgg actaccaatt tttgtaattg taccatgttc taaaacaact acgtgcgaag
     1081 caagtttata tatttcgtga atatcatgac ttactataat tgtagtaata ttgtataact
     1141 tttgaacttt acggatttca ttttgtaatt gtaaccgcat taccgaatca agggcagaca
     1201 agggttcgtc gagcaataat aattgcggat tgcgcgccaa cgcacgagca agtgctactc
     1261 gttgtttttg tccaccggaa aggcctgcag gttttctatt tttaaattcc aacaacccaa
     1321 atactgtaag aatttcggta actttttgta catcttttgt attttgtgca aaaagtaaat
     1381 tttgctctac tgtcatattc ggaaacaatg cataatcttg aaacatataa ctgatgtttc
     1441 gttgttgcgg tggtatttgt attttttttg ccgaatcaaa ccaaatggta ttatcaaaca
     1501 ctattgagcc tttgtcgggt tgcaagagac cggctaaaat tctgagcaag gttgttttac
     1561 ctgcacccga ttgtccgaac aatgcagtaa gttcatgagc tttgattgat gtttgcaccg
     1621 aaagagggac aggtccttcg gcggtatgca tcattttttc aatatctatt tcaattatat
     1681 gattttccat gtgtcatgtt ttttgtttac gctgtaaata gtagtaagaa gtataagtga
     1741 gagcatgaat aatataatag catactgatt ggcagtatca aagttgaggg cttgtacctc
     1801 atcgtaaata gcaattgaag ctactcgggt ttggcccggt aaattacctc caaccattag
     1861 aacaacacca aattcaccta tagtatgtgc aaatgttaga gccactgcgg ttataattga
     1921 tggtttaata ttgggtaaca gaactctaaa aaatgtggta gtttttgatt ttccaagcgt
     1981 atatgatgcc tcgcgaatac tatcggggag tgaacgtaat ccgttttgta gaggttgcac
     2041 cataaatggt aagctaaaca ataccgatgc tatgagaata ccttcgaaag aaaatgctaa
     2101 tcgtattcca aattcattaa aaagccatcc tccaaaccaa tgatttggac tgtatgccac
     2161 aagcatatag tatcccaata cagaaggggg taaaaccata ggcatactta ttaaggcctc
     2221 aaacagtggt tttaatttaa actttgaata ggtaagccaa taggctaaag gcaatccaat
     2281 accaattgca atacacgttg tgcacacagc caattctgcg gttaatacaa gtgtttgaat
     2341 aaatatatgt tccatagttt ttattgttt
//
LOCUS       DAOTOW010000334         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35080_length_2367_cov_3.654844, whole genome shotgun sequence.
ACCESSION   DAOTOW010000334 DAOTOW010000000
VERSION     DAOTOW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35080_length_2367_cov_3.654844"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1672)
                     /gene="guaA"
                     /locus_tag="P5243_06625"
     CDS             complement(143..1672)
                     /gene="guaA"
                     /locus_tag="P5243_06625"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRS19158.1"
                     /translation="MEKILILDFGSQYTQLIARRVRELNVYCEIVPFHKIPKIDSTIK
                     GVILSGSPYSVNDTDAPMPNLDFCKGKLPVLGVCYGAQLMAKQFGGNVQPSKTREYGR
                     AHLTYIETNNILFQSISHNSQVWMSHGDTITEIPSNYEIIASTEDVKVAAYHIQGEQT
                     FGIQFHPEVYHSTDGATLLKNFIVEICKCSQSWTPDSFIESTIAELKQKLGNDKVVLG
                     LSGGVDSSVAAVLLHRAIGKNLICIFVDNGVLRKNEFESVLEQYKVLGLNVIGVNAKD
                     QFLDKLEGVSDPETKRKIIGGTFIEVFDQEAHKLEDVTWLAQGTIYPDVIESVSATGG
                     PSATIKSHHNVGGLPDFMKLKIVEPLRLLFKDEVRRVGKALQIPTDILARHPFPGPGL
                     AIRIIGEVTREKVAILQECDAIFINGLKTHGLYDSVWQAGVMLLPVQSVGVMGDERTY
                     ESCVALRAVSSTDGMTADWCHLPYEFLAKMSNEIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            complement(1844..>2367)
                     /locus_tag="P5243_06630"
     CDS             complement(1844..>2367)
                     /locus_tag="P5243_06630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRS19159.1"
                     /translation="TSSVKVRVMEHQKPPIRAVFPGRVFRNEAISARAHCIFHQVEGL
                     YIDTNVSFADLKQTLLYFAKEMFGVDTQIRLRPSYFPFTEPSAEMDISCSICGGKGCN
                     VCKYSGWVEILGCGMVDPNVLENCGIDSKIYTGFAFGMGVERITMLKYQIKDIRLFFE
                     NDIRFLRQFEAAL"
BASE COUNT          768 a          471 c          325 g          803 t
ORIGIN      
        1 aaaaaacctc caaaaataat tatattactt ttttttaaaa ttatcaaatt tttttaaaaa
       61 aaacattgca cgatgttatg aatttttaca aacaaaaaaa ggcatatttt catatacctt
      121 ttttctatac tcatatattt ttctactccc actctatagt tgcaggtggt tttgagctaa
      181 tatcatacac tactctattt acacctttca ccttgttgat aatttcattc gacatttttg
      241 ctaaaaattc atacggtaaa tgacaccaat cggcagtcat accatcggta gagcttacgg
      301 ctcgaagtgc tacacaactt tcataagtac gttcatcacc cattactccc actgattgca
      361 caggtaacag cataacacct gcttgccaca cgctgtcgta tagtccatga gttttgagtc
      421 cgtttataaa aatagcatca cattcttgta atatagctac tttttcgcga gtaacttcgc
      481 ctataatacg aattgctaat cccggaccgg ggaagggatg acgagccaaa atatcggtag
      541 gtatttgtaa agccttgcct actctacgaa cttcatcttt aaataataat cgtaatggtt
      601 ctacaatttt aagtttcata aaatcgggca acccgcctac attatggtgc gatttaattg
      661 ttgcactcgg tccaccggta gccgaaaccg actctataac atcgggatat atagtaccct
      721 gcgccaacca tgttacatct tcgagtttat gtgcttcttg gtcaaatact tctataaagg
      781 taccacctat aatttttcgt tttgtttcgg ggtctgacac tccttcgagt ttatccaaaa
      841 attggtcttt tgcatttacc cctatcacat ttaatcctaa tactttatat tgttcgagaa
      901 ccgattcaaa ttcgttctta cgtaatactc cattatcaac aaaaatacaa attaaatttt
      961 tgcctattgc tctatgtaat aatacagctg caacactcga atcaactcca cctgataatc
     1021 ctaatacaac cttatcattt ccaagttttt gtttaagttc ggcaattgta gattctataa
     1081 acgaatcggg cgtccacgat tgagaacact tacaaatttc tacaataaag tttttcaata
     1141 gtgttgcacc atcggtagaa tgatatactt ctggatgaaa ttgtattcca aaggtttgtt
     1201 cgccttgaat atgatatgcg gcaactttta cgtcttcggt gcttgctata atttcgtaat
     1261 ttgaaggaat ttcagtaata gtatcgccat gcgacatcca cacttgtgaa ttatgagaaa
     1321 tactttgaaa taaaatatta ttagtttcaa tatatgtaag atgagctctt ccgtattctc
     1381 ttgtttttga cggttgcacg tttcctccaa attgctttgc cataagttgt gcaccataac
     1441 acacacccaa tacaggcaat tttcctttac aaaaatcaag atttggcata ggtgcatctg
     1501 tatcatttac cgaataagga cttcccgata aaattactcc ctttattgta gaatctattt
     1561 taggaatttt gtgaaatggc acaatttcac aatacacatt taactctcgt actcttctgg
     1621 ctatcaactg tgtatactga gaaccaaaat ctaaaataag tattttttcc attaacaatt
     1681 ataatttgaa gtcaaaaata gttttttttt acctattctt tagaagtaac agtaaaattt
     1741 ttaaaccata tttatcaggt aagtaaataa tcaaatattt tgagttattg taaagaataa
     1801 cactgttaca ataagattta ctgcaaaaaa atgtttcatt atattacaat gcagcttcaa
     1861 actgccttaa aaatcgaata tcattttcaa aaaacaatcg aatatcttta atctgatatt
     1921 taagcattgt tatacgttct acccccatac caaaagcaaa acctgtatat attttactat
     1981 ctataccaca attctcaagc acattaggat ctaccatacc acaacctaaa atttcaaccc
     2041 aaccgctata tttacatacg ttacaccctt ttcctccaca tatagagcat gaaatatcca
     2101 tctctgccga tggttctgta aatgggaaat atgatggtcg tagacgaatt tgcgtatcaa
     2161 ctccaaacat ttcttttgca aaatacaata atgtttgctt taaatcggca aatgaaacgt
     2221 ttgtatcaat ataaagtcct tctacttgat gaaaaataca atgtgctcga gcagaaattg
     2281 cttcattacg aaatactcta ccaggaaata cagctcgaat tggaggtttt tgatgttcca
     2341 taactctaac cttaacagaa gatgtat
//
LOCUS       DAOTOW010000335         2366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35103_length_2366_cov_3.104284, whole genome shotgun sequence.
ACCESSION   DAOTOW010000335 DAOTOW010000000
VERSION     DAOTOW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35103_length_2366_cov_3.104284"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="P5243_06635"
     CDS             <1..156
                     /locus_tag="P5243_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRS19160.1"
                     /translation="RSKKTDDEYKGRNSQNKTIVFPRANTKIGDFVKVKVISASSATL
                     KGEVVVE"
     gene            complement(162..2123)
                     /locus_tag="P5243_06640"
     CDS             complement(162..2123)
                     /locus_tag="P5243_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRS19161.1"
                     /translation="MYKHPKALVILFFTEMWERFSFYGMRALLTLYLTLQLFKHLQEP
                     EKKATAFGIYAAYGALVYATPFLGGLIADKFLGHKKAVIWGAILMAIGHFAMAIETEF
                     WLYIALSFLILGNGFFKPNISSIVGELYEQNDPRRDGGFTIFYMGVNLGAFLSPLVCG
                     TIGELYGWKYGFGIAGFGMIAGLFIFLQWKHWLNIHDKVIETETSYLVTQEIKPVGEP
                     PSQEKLQKPLFLGISTEYIIYIVSFASVALFALMVKHYELMTYVLTPFSLLVFGIIIV
                     VAMRSPKIERERLFVILILLLFTTLFWAFFEQAGSSLTLFANENVDRNLFSWQIPASL
                     FQSVNPLFIIIFASPFTILWLWLASKKKEPSTPIKFAWGILLLSLGFLVLAIAPLFVA
                     QTSLHIEQGGISWLIQAAAVPMSILIISYFLQTLGELCLSPIGLSMVTKLASPRIVGM
                     VLGAWFLSSAMAHHIAGFIAKATAEQQTSIEVMYNSFVTNIPTNAIDISSEEFYKTYK
                     GALSTGFNRGFDVIQQQGNSLYISATTEDFNTTVIIDSILAVCNTHHMNTHITNDSLF
                     RKSLYIAVIKGIEHMKESGTHAVTYAINKDYINTQTADSYSITTRYSLSNLLQYAKVF
                     LAVGLIALAASALLFALSPIIKRMMHGIG"
     gene            complement(2135..>2366)
                     /locus_tag="P5243_06645"
     CDS             complement(2135..>2366)
                     /locus_tag="P5243_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRS19162.1"
                     /translation="NIEKFLEDTQKHICGKVFIKLYPYNFDIIGIESKYDMMQAKFAQ
                     YGEMNTAFSGEDVKGFTKILSNQIKLYNSVED"
BASE COUNT          812 a          390 c          393 g          771 t
ORIGIN      
        1 agaagtaaaa agaccgacga tgagtataaa ggaagaaatt cgcaaaataa aacgattgtg
       61 tttcctcgtg ctaataccaa aattggtgat tttgtgaaag ttaaggtaat ttctgcaagt
      121 tctgcaacac tcaaaggtga agtggttgta gaataaataa tttatcctat accatgcatc
      181 atacgtttta ttataggcga aagtgcaaat aatagggcag atgctgccaa cgctattaat
      241 cctacagcaa gaaaaacttt agcatattgc aataaatttg ataatgaata ccgtgttgta
      301 atagaatatg aatctgctgt ttgtgtgtta atatagtctt tgtttatagc atatgttaca
      361 gcatgtgttc ccgattcttt catgtgttct atacctttta ttacagctat atatagcgat
      421 tttcgaaata gggaatcgtt ggtaatatga gtattcatat ggtgtgtatt gcatacagca
      481 agtatagaat ctataattac tgtggtatta aaatcttcag tagttgccga aatatataat
      541 gaattacctt gttgttgaat cacgtcaaat ccgcgattga atccggttga taaagctcct
      601 ttataagttt tgtaaaattc ttcggaactt atatcaatag catttgtagg aatatttgtt
      661 acaaaactat tgtacattac ttctatactt gtttgttgtt cggcggtagc ttttgcaata
      721 aatcctgcta tatggtgagc cattgccgat gatagaaacc aagctcccaa caccattcct
      781 actatacgtg gcgatgccaa tttggtaacc atagaaagac caattggtga caagcataat
      841 tctcctaatg tttgtaaaaa ataactgata ataagaatac tcataggaac agccgctgct
      901 tgtataagcc atgatatgcc accttgttct atatgtaatg atgtctgtgc aacaaacagt
      961 ggtgcaatgg caagtactag aaatcctaaa ctaagtaaaa gtatacccca agcaaattta
     1021 ataggagttg aaggttcctt tttttttgat gccaaccata accacagtat ggtaaatggg
     1081 ctggcaaata tgataataaa taatggatta accgattgaa ataatgatgc aggtatttgc
     1141 catgaaaaaa gattacggtc aacattttcg ttagcaaata acgttagtga tgaaccggct
     1201 tgttcaaaaa acgcccaaaa taaagtagta aataagagta aaatgagaat gacaaataaa
     1261 cgttcgcgtt ctatttttgg actacgcatt gctacaacta ttataatacc aaacaccaat
     1321 aacgaaaatg gtgtaagaac ataggtcata agttcgtaat gtttcaccat aagagcaaaa
     1381 agagctacag aagcaaaact tacaatatat ataatatatt cagttgaaat tcctaaaaat
     1441 aatggttttt gtaatttttc ttgtgaaggt ggttctccaa caggttttat ttcttgtgtt
     1501 actaaatatg atgtttcggt ttctattact ttatcatgaa tgttgagcca atgtttccat
     1561 tgtaagaata taaataggcc ggcaatcatt ccaaatccgg ctattccaaa accatatttc
     1621 catccatata attcgccaat ggttccacat acaagcggag ataagaatgc gcctaaattt
     1681 actcccatat aaaatatggt aaatccccca tcgcgtcttg ggtcattttg ttcgtacaat
     1741 tcaccaacaa ttgaagaaat attaggttta aaaaaaccat tccccaatat taaaaatgaa
     1801 agtgcaatat agagccaaaa ttctgtttct attgccatgg caaaatgtcc tatagccata
     1861 agaattgcac cccatattac cgctttttta tgacctaaaa atttatcggc tatcaaacca
     1921 cctaaaaaag gagtagcata taccaatgca ccatatgcgg catatatacc aaacgcagtt
     1981 gctttttttt cgggttcttg taaatgttta aataattgaa gagttaaata taaggtaagt
     2041 aacgcccgca tgccgtagaa actaaatcgt tcccacattt ctgtaaagaa cagtataact
     2101 aaggctttag ggtgtttata catagttttt aaatttagtc ttctactgaa ttatatagtt
     2161 ttatttggtt cgataaaatt ttggtaaaac cttttacgtc ttcacccgag aatgccgtat
     2221 tcatttcgcc atattgagca aatttagctt gcatcatatc gtattttgat tctataccaa
     2281 taatatcaaa attgtacgga tataatttta taaatacttt accgcatata tgtttttgag
     2341 tatcttctaa aaatttctca atatta
//
LOCUS       DAOTOW010000336         2361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35201_length_2361_cov_2.813530, whole genome shotgun sequence.
ACCESSION   DAOTOW010000336 DAOTOW010000000
VERSION     DAOTOW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35201_length_2361_cov_2.813530"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /locus_tag="P5243_06650"
     CDS             complement(<1..1804)
                     /locus_tag="P5243_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS19163.1"
                     /translation="MQRHLLLIIYQIFIISGLTAQSLSNQKIFEFTPEFGLSNGKVHD
                     ITQDLHGFIWIATEEGVTRYDGKQVKKYVVQEQDPKYGLSHNNVYALAADNNYGVWIG
                     TSAGLDAYNHKTGTFSKYSFYTKQGTPLLKPVWQIVPFANNVCILRSDSKELYVYKPG
                     NDTIKAIVSPSFKPTHIPNFIDSIASQTVVVGDKGGLLYTLHASGRVIIIDTLPHSIS
                     CVKMLINNILAVADVYGNLYFYKNNNCISTIQFPNQKVSNSEKNISSIEQISSDELII
                     GTRGSGVFRVLISAKKIEELSYNSEIINQHVEKVFKDSFQNMWFAHSYGGVSVALNQY
                     QIFGLLQLPQQVYEQKILTIYKQANILYIGTDGNGMFIYNTKNKQLQQVNYETGYKGY
                     EFDNVVTSISSDGKRIWIATYNKGIFAIDIATNSLVYIDELKQIPASNFSNIYCDTQQ
                     KVWIGTYEHGVYVFDIQKKKIIKHYTSMPENSPEALSCNGVTCFFEDASKNMWIGSYY
                     GISKIHSNGFVTRYTVQNHKGLKNNFITTFAQDTKGNIWIATKQGLSVYNSKQDSLHV
                     FELNHYLHKKQIQSLYMQDSILWIVSEYSLFSMNL"
     gene            complement(1858..>2361)
                     /locus_tag="P5243_06655"
     CDS             complement(1858..>2361)
                     /locus_tag="P5243_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HRS19164.1"
                     /translation="ISPVKFYKPGVFTVNDFFEEDAYVIDLQNTHVAKAKNEVSTHDL
                     ENMLQQKIKQDSFVKPQPIVEKEHIREVDLHIHELVDNELGLDAAAKLEIQLQTFEKE
                     LAKAMTDGVEKIVFIHGVGNGVLKTKIRGILDRDYGHLLYQDASFQKYKFGATLIYIK
                     GATRKKR"
BASE COUNT          742 a          403 c          310 g          906 t
ORIGIN      
        1 gcaaattcat agaaaacaac gaatattctg atactatcca cagaatacta tcttgcatat
       61 acagagattg aatttgtttc ttgtgtaaat aatgattcaa ttcaaataca tgcaaagaat
      121 cttgttttga attatataca cttaatcctt gttttgttgc aatccatata ttaccttttg
      181 tatcttgggc aaatgtggtt ataaaattat tttttaaacc tttatgattt tgaactgtat
      241 atcgtgtaac gaatccattt gaatgtattt ttgaaatacc gtaatatgaa ccaatccaca
      301 tattttttga tgcatcttca aaaaaacatg ttacaccatt acaactcaat gcctcaggag
      361 aattttcagg catagacgta taatgtttta ttatcttttt cttttgtata tcaaatacat
      421 acacaccgtg ttcataggtt cctatccata ctttttgttg cgtatcacaa taaatatttg
      481 aaaaattact tgccggaatt tgctttaact catctatata tactaacgaa tttgttgcta
      541 tatctatagc aaatatacct ttgttatagg ttgcaatcca tattcttttt ccatcagaac
      601 taattgatgt tacaacatta tcaaattcat atcctttata tcctgtttca taatttacct
      661 gctgtaactg tttatttttt gtattatata taaacatacc atttccatcg gtacctatat
      721 aaagtatatt tgcctgcttg tatatagtta atattttttg ttcatatact tgttgaggta
      781 attgcaatag tccaaatatt tgatattgat tcaaagcaac actaacccca ccatatgaat
      841 gagcaaacca catattttga aatgaatctt taaaaacctt ttctacatgt tggtttatga
      901 tttcggaatt atatgaaagc tcctcaattt tttttgcaga aattaatact ctaaacacac
      961 ccgaacctct agttccaatt attaattcat cacttgaaat ttgttcaatt gatgaaatat
     1021 ttttttctga atttgatact ttttgattag gaaattgaat agttgatata cagttgttat
     1081 ttttgtaaaa atagagattg ccatacacat cggcaacagc caatatatta tttatcaaca
     1141 ttttaacaca cgaaattgaa tgtggcagtg tatctattat tattactctt cctgaagcat
     1201 gcaaagtgta caataaacct cccttatcgc ctacaactac tgtttgcgaa gctatagaat
     1261 ctataaaatt aggaatatgt gtcggtttaa atgagggcga tacaatagct tttattgtat
     1321 catttccggg cttatacaca tacaattctt ttgaatcact gcgaagaata catacattat
     1381 tagcaaacgg cacaatttgc cataccggct ttagaagtgg tgttccttgt ttagtataaa
     1441 atgaatattt tgaaaatgtt cctgttttat gattatacgc atctaaacct gcagaagtac
     1501 caatccatac tccataatta ttatcggcag ctagtgcata tacattatta tgcgacaatc
     1561 catacttagg atcttgttct tgaactacat actttttaac ttgtttacca tcatatcgtg
     1621 taaccccttc ttctgttgct atccatataa atccatgtaa atcttgtgtt atatcatgaa
     1681 ctttaccatt tgataatccg aattcgggag taaattcaaa tattttttga tttgagagcg
     1741 attgagcagt caaaccactt attataaata tttgatatat aataaggagt aaatgtcttt
     1801 gcataatttg tattcttatt acaaagtaac tacatagttt ttaatttaaa aataatttta
     1861 cctttttttt ctcgttgctc ctttgatata tatcaatgtt gctccaaatt tatatttttg
     1921 gaacgaggca tcttgataca ataaatgccc gtaatctctg tcgagtatgc cacgaatttt
     1981 ggttttgagt accccattgc ctacaccatg aatgaataca attttttcga ccccatcggt
     2041 cattgctttt gctaattctt tctcaaatgt ttgcaattga atttccaatt ttgcagcagc
     2101 atcaagcccc aattcattgt ctaccaattc atgaatatgc aaatctactt cgcgaatatg
     2161 ttctttttct actattggtt gtggttttac gaatgaatct tgtttgattt tttgttgcaa
     2221 catattctct aaatcatgag ttgaaacctc attttttgct ttagccacat gtgtattttg
     2281 taaatcaatg acatacgcat cttcttcgaa aaaatcattt accgtaaaca ctccgggttt
     2341 ataaaatttt acgggagaaa t
//
LOCUS       DAOTOW010000337         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35239_length_2359_cov_3.123698, whole genome shotgun sequence.
ACCESSION   DAOTOW010000337 DAOTOW010000000
VERSION     DAOTOW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35239_length_2359_cov_3.123698"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1436)
                     /locus_tag="P5243_06660"
     CDS             complement(102..1436)
                     /locus_tag="P5243_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS19165.1"
                     /translation="MQIIQNIRSRLFLFRSILYAQYIYNILLNKKYKKLVSYSVNYKK
                     IIHINTADKGGGAAKIASDVCNYQQTHGYNSVLLVDEKKTISHNVFAIQQRHSKKQDI
                     LYAAQKYLQWQDFFHLSSFKLLEYPQVKDADIIHLHNLHSWNGYFSIFALPELCSRKQ
                     VVWTLHDMQLLTGHCAYSFDCEKWEIGCGKCPYLDIYPKLTKDTTAFIWKTKKRIYQQ
                     CDMHIVVPSKWLQNIVSQSILSHLSCSLIYNGIDITIYKPHNKEDVRNKFNIPETAYV
                     VLFSADLGINNPFKGGEFIKQLIEYYQLYSNILFVNIGGGNSIVKYDKIWNIPYVQNE
                     QEMSYWYSAADVYVYPSLADNCPLVILEAMACGLPIVTFETGGIPELVLHNETGYVAK
                     YKDFEDLKRGINLLIHNSNIRKQYSINSINRVKNYFTKEIMIQNYLTLYNNL"
     gene            complement(1438..>2359)
                     /locus_tag="P5243_06665"
     CDS             complement(1438..>2359)
                     /locus_tag="P5243_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19166.1"
                     /translation="ILFLIFNRIDTTKQVFFEIRKQKPKQLFIAADGARKHKIGEDEE
                     CKRIRKWVIENIDWECEVITRFQIENQGCGKHVSGAISWFFEHVEMGIILEDDCVPNY
                     SFFKFCEQLLDMYKHNHKVYMIGGNNFQISPKHTYSYYFSTIGHIWGWATWRRAWQKY
                     DFTLQYISDKYFKKQANNFFKNSQMVDFWYRTFKTMKYTPIDTWDYQWSITLIVHGGL
                     AITPYVNLVSNIGFNADATHTISYIDGISNLQTHELQTIVHPSKITINRKADLFTFYK
                     IFERKKKNMFIKKCKDKLKYEYTKIIQRFQ"
BASE COUNT          824 a          365 c          289 g          881 t
ORIGIN      
        1 gaaaaaaatg aatcattaat aaatttatct aaaaaattag aatcagaatc ttggactaga
       61 caagagctat ttaaaaaata ttttggtttt aaatatttat actacaaatt attatataat
      121 gtcagatagt tttgaatcat tatttctttt gtaaaataat tctttactct atttatagaa
      181 tttatagaat attgtttgcg tatatttgaa ttatgtatta gtaaattaat tcctcgtttt
      241 aaatcttcaa aatctttata ttttgcaaca tatcccgttt cgttatgtaa aactaattct
      301 ggaatacccc ctgtttcaaa tgttacaata ggcaacccac aagccattgc ttctaaaatt
      361 actaacggac aattatctgc taaacttgga tacacataca catcagcagc tgaataccaa
      421 taagacattt cttgttcatt ttgaacatat ggaatattcc aaattttatc atatttaact
      481 atagagttac ctccaccaat atttacaaaa agtatatttg aatataattg ataatattct
      541 attaattgct taatgaattc gcctccttta aatggattat taatacctaa atcagctgaa
      601 aataaaacca catatgcagt ttccggtata ttaaatttgt tgcgaacatc ttctttatta
      661 tgaggtttat atatagtaat atctattcca ttataaatca aagaacaaga taaatgtgat
      721 aaaatacttt gtgaaactat attttgcaac catttgcttg gcactactat atgcatatca
      781 cattgttgat atattctttt tttagttttc caaataaatg cagtagtatc cttggtaagt
      841 ttaggatata tatctagata aggacattta ccgcatccta tttcccattt ctcacaatca
      901 aacgaatatg cacaatgccc tgttagtagt tgcatatcat gtaatgtcca aactacctgt
      961 ttgcgagaac atagttctgg caaggcaaat attgagaaat aaccattcca agaatgaaga
     1021 ttatgtaaat gaattatatc tgcatctttt acttgaggat attctaataa tttgaagctt
     1081 gaaagatgaa aaaaatcctg ccattgcaag tatttttgag cggcataaag tatatcttgt
     1141 ttttttgaat gtctctgctg tatagcaaaa acattatgag atattgtctt tttttcgtct
     1201 accaacagaa cagaattata tccatgtgtt tgttgataat tacatacatc agaagcaatt
     1261 tttgctgcac caccaccttt gtctgccgtg tttatatgta ttattttttt gtaattgaca
     1321 gaataagaaa ctaatttttt atatttctta ttcaatagta tattatatat atattgagcg
     1381 tacaaaattg aacgaaataa aaataaacgt gaacgtatat tctgaattat ctgcatatta
     1441 ttgaaacctt tgtattatct ttgtatactc atattttagt ttatccttac attttttgat
     1501 aaacatattt ttctttttac gttcaaaaat tttgtaaaac gtaaataaat ctgctttacg
     1561 attaattgta atttttgaag gatgaactat tgtttgtaat tcatgagttt gtaaattaga
     1621 aataccatct atatatgaaa tagtatgtgt agcgtcggca ttaaacccaa tatttgaaac
     1681 tagatttaca tatggagtaa ttgcaagtcc cccatgtaca attaatgtta tagaccattg
     1741 ataatcccaa gtatctatag gagtatattt cattgtttta aatgttctat accaaaaatc
     1801 aaccatttga gaatttttaa aaaaattgtt tgcttgtttt ttaaaatatt tgtcagaaat
     1861 atattgtaaa gtaaaatcat atttttgcca tgctctacgc catgtcgccc acccccaaat
     1921 atgaccaatt gttgaaaaat aatatgaata tgtatgcttt ggggaaatct gaaaattatt
     1981 gccaccaatc atataaacct tatgattatg tttatacata tctaataatt gttcacaaaa
     2041 tttaaaaaaa ctataatttg gcacacagtc atcttctaaa attattccca tttcaacatg
     2101 ttcaaaaaac caggaaatag cacccgaaac atgtttgcca caaccttggt tttctatttg
     2161 aaaccgagtt ataacctcac attcccaatc aatattttcg ataacccatt ttcttattct
     2221 tttacactct tcgtcttcgc caattttatg ctttcgtgcc ccatcagcag ctataaataa
     2281 ttgcttgggc ttttgcttcc taatttcaaa aaaaacctgc ttagtcgtat caattcgatt
     2341 aaaaataaga aataaaata
//
LOCUS       DAOTOW010000338         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35258_length_2358_cov_3.265306, whole genome shotgun sequence.
ACCESSION   DAOTOW010000338 DAOTOW010000000
VERSION     DAOTOW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35258_length_2358_cov_3.265306"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2358
                     /locus_tag="P5243_06670"
     CDS             <1..>2358
                     /locus_tag="P5243_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS19167.1"
                     /translation="TATLSSARIPLVTAVANNPNALVEVVYATNTTQNTVITVTAESG
                     AKQVYTIAHSYSNPSQTIYSWSDNFDDNLATDFTVTGTSATYYTITAQNQEILVSQAA
                     GKTAYRTFIYNLPSGYLLDMLNNSNPILTFSVRSAKAVDIRVDLRDADGNVGSTNIVT
                     KSVAAGASTTLTYDYTGKNTNIDKSRVVALVFYTDPDITVNEAKQIYIDNITLGIALS
                     NNANLATLTASAGTLSPTFNANTTSYTLTLPAGTTTIPTISATKAQTNATLEISQPNN
                     LSGTAIVRVTAQDNVTVKTYTVQLVQTPQTVLGFTDHIIKPGMPGWTENSSLYSLQYL
                     GGEIAVSYYRTAAGGSDAITYNITDAEAKILNLTANPYVAIRLKTTVQTDLRVDLFDN
                     NGYVTNSSPVIKSANGNTYVDYIFNFSEKFSQTSPTQTVASNNIRGIKMYFDNGSTTP
                     KSGTITIDKLIFGNEVVIPVNNPPVISTIPNQSIMQGETFNNVLLNNYVTDDSTPVTS
                     LIWSVNTSTNFNVTITNNVATIVPKNSAWIGSETLTFTVQDLDGATASKTATFTVTEL
                     KIPVENVLFTQTSVNVAQNATIDLSGYLSITPTNATIQTITWTESSANASINTAGVLT
                     NSLQWGTEAVTVTVTVTDKNSNTFTKTITVNLTGCPTQLTSVSLLPATVSVVEGQTIQ
                     LTPTYTPANACIKTVTYSSSATSVATVSASGLVTGIAPGTAIITISVNDGFTTKTATK
                     TVTVTKDCSGAIVMSISQSSVSLIAGTNTTLSVSFSPNNECTQNKV"
BASE COUNT          822 a          407 c          400 g          729 t
ORIGIN      
        1 tactgccaca ttatcgagtg cacgaatacc attagtaact gcggttgcaa acaatccaaa
       61 tgcgttagtt gaggtggtgt atgctactaa tacaactcaa aatactgtta ttaccgtaac
      121 agccgaaagt ggtgcaaaac aagtatatac tattgctcat agttattcaa atccatcgca
      181 aactatttat agttggagtg ataattttga tgataattta gctacggatt ttacggttac
      241 aggtaccagc gctacatatt atactataac agctcaaaat caagaaattc ttgtatcaca
      301 agctgccggg aaaacggcat atcgtacatt tatttataat ttaccttcag ggtatttgct
      361 cgatatgctt aataattcta atcctatttt aacatttagt gtaagaagtg ctaaggctgt
      421 tgatataagg gttgatttgc gtgatgcaga tggtaatgtt ggtagtacaa atattgttac
      481 aaaatctgtt gctgcagggg catctacaac attgacgtat gattataccg gaaaaaatac
      541 aaatattgat aaatcaagag tggttgcatt ggtgttttat accgacccgg atattacagt
      601 aaatgaagca aaacaaattt atatagataa tattacatta ggaattgctt tgtcaaataa
      661 tgcaaatttg gcaacattaa cggcaagtgc aggaacactg tcgcctactt ttaatgcaaa
      721 tactacaagt tacaccctta ctttacctgc aggcactaca actattccaa caatatctgc
      781 tacaaaagca caaacaaatg ctaccttaga aataagtcaa ccaaataatt tgtcgggaac
      841 ggcaattgtt agagtcacag ctcaagacaa tgtgactgta aaaacctata ccgtgcaatt
      901 agtacaaact cctcaaactg tattaggatt tactgaccat attataaaac cgggtatgcc
      961 gggttggaca gaaaattcat cattgtatag tttacagtat ttaggaggcg aaatagcagt
     1021 gtcttattat agaactgctg ccggaggtag tgatgctata acatataata taaccgatgc
     1081 tgaagctaaa atattgaatc ttacagcaaa tccatatgtg gctattcgat taaaaacaac
     1141 tgtgcaaaca gatcttagag tagatttatt tgataataat gggtatgtta ccaattctag
     1201 tcctgttata aaatcagcaa atggcaatac atatgtagat tatattttta atttttcaga
     1261 gaaattctcg caaacatctc ctacacaaac agttgcctca aataatatac gtggaattaa
     1321 aatgtatttt gataatggat ctactactcc aaaatcgggt acaataacta ttgataaact
     1381 tatttttgga aacgaagttg taattcctgt taataatcct ccggtaattt caactatacc
     1441 aaatcaatca attatgcaag gggaaacatt taataatgtt ttgctcaata attatgtaac
     1501 cgatgatagt acacctgtta cgtctcttat ttggtcggtt aatacttcaa ctaattttaa
     1561 tgttactatt acaaacaatg ttgctactat agtaccaaaa aattctgcgt ggataggtag
     1621 tgaaacactt acatttacag tacaagattt agatggagca acagcatcaa aaacagctac
     1681 gtttacagtt actgagctta aaatacctgt agaaaatgtt ctgtttacac aaacatctgt
     1741 taatgtagcg caaaatgcta ctattgattt atccggctat ttgagtataa ctcctacaaa
     1801 tgcaacaatt caaacaatta catggactga atcaagtgca aatgcttcta taaatacagc
     1861 gggagtattg actaattctt tgcaatgggg tacagaagct gtaacggtaa cagtaacggt
     1921 aacagataaa aattcaaata cattcacaaa aactattacc gtaaatctta ccggttgtcc
     1981 tactcaactt acaagtgtga gtttattacc tgcaactgtt agtgtggttg aaggtcaaac
     2041 aatacaactt actcctacgt atactcctgc aaatgcatgt attaaaacag ttacgtatag
     2101 cagttctgct acttctgtag caacagtttc tgcttcggga ttggttacag ggattgctcc
     2161 gggaactgca attattacta tttcagtaaa cgatggattt actaccaaaa cagctaccaa
     2221 aacagttact gttacaaaag attgttcagg agctatagta atgagtattt ctcaaagctc
     2281 tgtatctctt attgcaggta caaatacaac attgagtgta tctttttctc caaataatga
     2341 gtgtacacag aataaagt
//
LOCUS       DAOTOW010000339         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35285_length_2357_cov_2.854909, whole genome shotgun sequence.
ACCESSION   DAOTOW010000339 DAOTOW010000000
VERSION     DAOTOW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35285_length_2357_cov_2.854909"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="P5243_06675"
     CDS             <1..309
                     /locus_tag="P5243_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19168.1"
                     /translation="TEVNIPEEGITLLEAIAQVGGLTPNSKAYKIKLIRGDNKNPEVY
                     NFNISSLEEFKKANFILQANDIIYVDSRPRYVTKVIAEIQPYLVLLSTSVLVYSIFSR
                     "
     gene            312..>2357
                     /locus_tag="P5243_06680"
     CDS             312..>2357
                     /locus_tag="P5243_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRS19169.1"
                     /translation="MAKQRKIPMFNDTFDMSKLVHILRKNFIFYIIFGLIAVISAFTY
                     LRYTPPIYEAYTILQINEEQENDLLKLDNVYGKTSINNLIELIRSREFLKRTLHKLNL
                     YTTYYLEGKFLTSELYNKTPFFVEYQDVQSAIYNKNIYMEYNKKDDNITIEYSIDGKN
                     YISHTIKRNEWTQLQGLKLYVNITNLNQVIENKGNKYYFRILDDKGAFAEHISGLNVS
                     ILNFDAQTIQIYYTSNNAQKTSDLVNTISEDFLSYNIEKKQERAQKVLAFIEDQMNIV
                     YSKLDESEKQLDQFKKTNNITGSIFDDKAAIQNALMDNSQQIDQYKNQLQSLQKVNSQ
                     IKSGQDFNTVELISILSGSSSESMILNFLNGIQQLQKQKEQLLITVTPDNHKVKIIDQ
                     QINEQKKLLLDFIKITNDRIKTELDAAKAKTKVEKAVEIDEIELAKLKRIHSINQNYY
                     DQLVSKRAEFMISKAGHVTNNLILQKSDIPQFPISPIPSFIIFVAAALAIMAMFILTL
                     VRYMLYNDITSLNDISRFTDVPVAGLIPVSKQTNKINRFVVENKANSVITEAFRTLRS
                     NLEFMHQVSNEKKIIVITSTISGEGKTFIAINLGGVLSMSGKRVILIDLDLRKPKVHL
                     TFKSNNEHGLSTILIGKDNYKDCIIETEFENFNIITSGPPPPNPAELANSESFDNLLE
                     "
BASE COUNT          974 a          348 c          306 g          729 t
ORIGIN      
        1 accgaagtaa acatacccga agaaggtatt acattacttg aagctattgc tcaagtaggt
       61 ggtcttacgc cgaatagtaa agcatataaa ataaaactta ttcgtggtga caataaaaat
      121 cctgaagtat ataattttaa tataagcagt ttagaagaat tcaaaaaagc taattttatt
      181 ttacaagcca acgatataat atacgttgat tctcgtcctc gatatgttac aaaagtaatt
      241 gcagaaattc aaccatattt agttttattg tcaacaagcg tattagtata tagcatattt
      301 agcagataag tatggcaaaa caacgtaaaa taccaatgtt taacgacacg tttgatatga
      361 gcaaattagt tcatatttta cgtaaaaact ttatatttta tatcattttt ggattaattg
      421 cagtaataag cgcttttact tatttacgat acactccacc tatttacgaa gcatatacaa
      481 ttcttcaaat taatgaagaa caagaaaatg acttattaaa acttgataat gtttatggaa
      541 aaacatctat taataatctt atagaattaa tacgttcacg tgaattttta aaacgaacat
      601 tacacaaatt aaatttatac actacatatt atttagaagg aaaattttta acttcagaat
      661 tatacaataa aactccattt tttgttgaat atcaagatgt tcaatctgcc atatacaata
      721 aaaatattta tatggaatat aacaaaaaag atgataatat aaccatagaa tattctattg
      781 acggtaaaaa ttatatttca catacaatta aacgtaatga atggactcaa ctacaaggtt
      841 tgaaattata tgttaatata actaacttaa atcaagtaat tgaaaacaaa ggtaacaaat
      901 actactttag aatattagat gacaaaggtg cttttgccga acacatcagt ggattgaatg
      961 tttcaattct caattttgat gctcaaacaa ttcagatata ctataccagt aacaatgcac
     1021 aaaaaacttc agatttagta aatactatca gtgaagattt tttaagttac aatattgaaa
     1081 aaaaacaaga acgcgctcaa aaggtacttg cttttattga agatcaaatg aatatagtat
     1141 acagcaaact tgatgaatca gaaaagcaac tcgatcaatt taaaaaaaca aacaatatta
     1201 caggaagcat ttttgatgac aaagcagcta ttcaaaacgc actgatggac aattcacaac
     1261 aaattgatca atataaaaat caattacaat cattacaaaa agtaaattca caaattaaat
     1321 caggacaaga ctttaataca gtagaactta tttcaatatt atccggttct tcaagtgaaa
     1381 gtatgatttt aaattttctc aatggaattc aacaacttca aaaacaaaaa gaacaattac
     1441 ttattactgt aactccagac aatcataaag taaaaattat agaccaacaa ataaatgagc
     1501 aaaaaaaatt attacttgat tttataaaaa taaccaacga tagaattaaa actgaattag
     1561 acgcggctaa agcaaaaaca aaagttgaaa aagcagtaga aatagatgaa atagaattgg
     1621 ctaaacttaa acgtattcat tctataaatc aaaactacta tgaccaatta gtttcaaaac
     1681 gtgccgaatt tatgatttct aaagcaggac acgtaacaaa caatttaata ttacaaaaat
     1741 ctgatatacc tcaatttcca atatctccta ttccatcgtt tataatattt gtagcagctg
     1801 cattagcaat tatggcaatg tttattctta cacttgtaag atacatgtta tacaatgata
     1861 ttacatcact caacgatatt agtcgtttta ccgatgtgcc ggtagcaggt ttaatccctg
     1921 tttcaaaaca aaccaataaa atcaatcgtt ttgttgtaga aaacaaagca aattcagtca
     1981 ttaccgaagc atttagaaca cttcgttcaa atcttgaatt tatgcatcag gttagtaatg
     2041 agaaaaaaat tattgtaatt acatcaacta tttcgggtga aggaaaaacg tttattgcta
     2101 ttaacttagg tggagtgctt tcaatgagtg gtaaacgagt aattttgatt gaccttgact
     2161 tacgaaaacc aaaagtacat ttaacgttta aatcaaacaa tgaacacggt ttaagtacta
     2221 tattaatagg taaagacaac tacaaagatt gtataattga aacagaattc gaaaacttca
     2281 atattattac atcaggtcct cctcctccaa acccggcaga attagctaac tcagaaagtt
     2341 ttgataattt attagaa
//
LOCUS       DAOTOW010000340         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35307_length_2356_cov_2.900478, whole genome shotgun sequence.
ACCESSION   DAOTOW010000340 DAOTOW010000000
VERSION     DAOTOW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35307_length_2356_cov_2.900478"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="P5243_06685"
     CDS             complement(<1..799)
                     /locus_tag="P5243_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS19170.1"
                     /translation="MKKIFLFVCVGFVITSCVSTKKYKALQDNCTSIQDSLRLELAAN
                     TKELIGLRERHLKLESESVALRADSASKNRTINNLTLENVELKKLNENLNAKQTKLVQ
                     QAALESRKMLEELQAARAILQKREDALDSIKLSLETERKALDKIRKELGIKNDEILIK
                     NKQLADMEELLRRKDSAAVALRTKIQKALVGFEGQGLTIEQRDGKIYVLLDEALLFKV
                     GKSDVSEKGQEALKKLATVLEQNPDINITVEGHTDNTGGEKLNWELST"
     gene            complement(896..>2356)
                     /locus_tag="P5243_06690"
     CDS             complement(896..>2356)
                     /locus_tag="P5243_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRS19171.1"
                     /translation="IQSFRDGFVKTVEYSRGTVLSNEPEAATTESNGVFVKFRPDDTI
                     FTNFAYRLEYVEEMLKNYTYLNAGLAILLNGKKFISKNGLLDLLQENLSSEALYDPIH
                     LKGEDIEVAITHENRYGEMYYSFVNGQNTIQGGTHLNAFREAYVKTIREFYNKNIEGV
                     DIRQSVIAAISIKVEEPVFESQTKTKLGSKDMSPNGVSVNKFINDFLKERLDNFLHKN
                     PLIADALLKKIQESERERKAMSGVQKKAKEMAKKANLHNRKLRDCRVHYNTNHELRNE
                     TCIFITEGDSASGSITKSRNANTQAVFSLRGKPLNTFGLTKKVVYENEEFNLLQAALN
                     IEEDIENLRYKHVIIATDADVDGMHIRLLLITFFLNFFPDLIKKGHVFILQTPLFRVR
                     NKKETRYCYSEEERLKAIEDLKPNPEITRFKGLGEISPDEFCHFIGKDIRLEPVKLSK
                     DMAISDLLTYYMGKNTQERQDFIISNLRIEEDVPVE"
BASE COUNT          703 a          454 c          297 g          902 t
ORIGIN      
        1 tagtacttaa ttcccaattc aatttttcac ctccggtatt atctgtatgt ccttcaacag
       61 taatatttat atcgggattt tgctccaaca ccgttgctaa ttttttcaga gcctcttgtc
      121 ctttttcgct tacatcactt tttcctactt taaacaacaa agcctcatct aataacacat
      181 aaattttacc atcacgttgt tcaatagtaa gaccttgacc ttcgaaacca actaaggctt
      241 tttgaatttt agttcgaaga gctacagccg cagaatcttt acgacggagc aactcttcca
      301 tatcagcaag ttgtttgttt tttataagaa tttcatcatt tttgattcct aattctttac
      361 gaattttatc aagagctttt cgttctgttt ctaaacttaa ttttatagaa tccaatgcat
      421 cctctcgctt ttgaagtata gcacgagcgg cttgtaattc ttcaagcatt tttctactct
      481 ctaaagctgc ttgttgcact aatttcgttt gctttgcatt taaattttcg ttcaattttt
      541 tcaattcaac attttctaat gttaaattat taatggttct atttttagat gcagaatcag
      601 cacgaagagc aacactttca ctttctaatt ttagatgtcg ctctcgcaat cctataagtt
      661 cttttgtatt agcggcaagt tctaaacgca gagaatcctg aatacttgtg caattatctt
      721 gcagagcttt atatttttta gtactaacac atgatgttat tacgaatcca acacaaacaa
      781 atagaaatat ttttttcatt gcagtttttt ttagtttcac aaaaataaca tttaaaacac
      841 taaacaatac agactgttaa catcttttaa taaataatat tgtatagcag ttactttatt
      901 ctaccggaac atcttcttca attcttaaat tactaattat aaaatcttga cgctcttgtg
      961 tgttttttcc catataatat gttaacaaat cactgattgc catatcctta ctcaatttaa
     1021 ctggttcaag tcgaatatct tttcctataa aatgacagaa ttcatctgga gaaatttcac
     1081 ctaatccctt gaatcgagta atctcgggat ttggttttaa atcttcaatt gctttcaatc
     1141 gttcttcttc gctatagcag tatcttgttt cttttttatt acgaactcta aaaagcggtg
     1201 tttgcaatat aaaaacatga ccttttttga ttaaatcagg aaaaaaattc aagaaaaaag
     1261 taattaataa caatcgaata tgcataccat caacatcagc atcggtagca attataacat
     1321 gcttatatcg taaattttcg atatcttctt caatattaag agctgcctgt aataaattaa
     1381 attcttcatt ctcatacacc acttttttag ttaacccaaa cgtattgaga ggttttcctc
     1441 gcaaactaaa tacagcttgc gtattagcat ttcttgattt tgtaattgac ccacttgccg
     1501 aatctccttc ggttataaaa atacacgttt cgttgcgcaa ttcatgattt gtattgtaat
     1561 gaactctaca gtcacgaagt tttctgttgt gcaaattggc tttttttgcc atttcttttg
     1621 cttttttttg cactcccgac atagcctttc gttccctctc cgactcttgt attttcttga
     1681 gcaacgcatc agcaattaat ggatttttat gtaaaaaatt atcaagacgt tcttttaaaa
     1741 aatcatttat aaatttatta acactcacgc catttggcga catatctttg gaccccaatt
     1801 ttgttttggt ttgtgattca aataccggtt cttctacctt tatacttata gctgcaatta
     1861 cactctgacg aatatcaact ccctctatat ttttattata aaattctcgt attgttttaa
     1921 catatgcttc gcgaaatgca tttaaatgtg taccaccttg aattgtattt tgtccgttta
     1981 caaaagaata atacatttcg ccatatctat tttcatgggt tatagcaacc tctatatctt
     2041 ctcctttcaa atgtatgggg tcataaaggg cttcactact taaattttct tgcaataaat
     2101 caagcaatcc gttttttgaa ataaattttt taccattcaa caaaatagca agcccggcat
     2161 taaggtatgt ataatttttg agcatttcct ctacatactc aagcctatag gcaaaatttg
     2221 taaatatagt atcatcaggt ctaaatttca caaatactcc attggattct gttgttgcag
     2281 cttcaggttc attagataat actgtacctc tactgtattc tactgtttta acaaatccat
     2341 cacgaaacga ttgaat
//
LOCUS       DAOTOW010000341         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35310_length_2356_cov_2.366797, whole genome shotgun sequence.
ACCESSION   DAOTOW010000341 DAOTOW010000000
VERSION     DAOTOW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35310_length_2356_cov_2.366797"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="P5243_06695"
     CDS             <1..374
                     /locus_tag="P5243_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19172.1"
                     /translation="RIELYSNVTLLKQKQAAKVSKAYLKQAREFLNQGNDAAYYEAIS
                     KALFGYIGNKLSIPMTDFTVETIADTLHKKQVPEIIIQTCIEIVNQCEMARYAPIQSI
                     EDKEQLFTKTSQVIQGIEQNV"
     gene            379..1140
                     /locus_tag="P5243_06700"
     CDS             379..1140
                     /locus_tag="P5243_06700"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRS19173.1"
                     /translation="MKTLYSIVFVILSITFVNANNQLNDSIIQNAQRLYKAQQYDSVI
                     VLYKSLISQGLVSDKLYFNLANAYYKQREIAQAILWYERAYVLNPYNEDIKNNLAIVQ
                     ALQPDKVEQIQQSMLQLWYPYVYRFCNSNTWAVLSLVFFIGFIVCVLWFLFTKQRVYK
                     KVSFFIALGSLGFAIITYINASYRYNELVKNQYAIVMESSLRILTEPNENAKELVVVH
                     GGLRVKIEKKNGDWVSIRLSDGKVGWVQHFAIEKI"
     gene            1237..>2356
                     /locus_tag="P5243_06705"
     CDS             1237..>2356
                     /locus_tag="P5243_06705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRS19174.1"
                     /translation="MRVDKEKIIAILQEYWGKTKYYSKILWTFYRAKDKKWYEKILHW
                     GAFVFSIILVYIIAVEINFLWLFGYTPNLSEINNPNQYVASEVYASNGKLLGKFFYEN
                     RTPVEYKDLSPLLIKTLIATEDARFYQHSGIDFRALAPVIMDALKGNPRGGSTLTQQL
                     VKNLFKTRNRNHGLLGKIPFLKIFIIKSKEWIGALKIELFFSKEDILTSYLNTVDFGS
                     NAYGIKTAAKTYFNTTPMRLQPEQCAVLVGMLKAPTAYSPILNKERSLSRRNVVLGIM
                     KRDNIITEREYEDLIKKPIVLHYNVEKNVHGIANYFRRAVFDYLKPWLKENDLDIYAD
                     GLKIYTTLDYNMQVYAEEAVAQNMKRVQRQFDAHWQGQN"
BASE COUNT          885 a          283 c          413 g          775 t
ORIGIN      
        1 agcgaataga attatatagt aatgttactc tgttgaaaca aaaacaagcc gctaaagtat
       61 ccaaagcata tcttaaacaa gctcgtgaat ttctaaatca aggaaatgat gcggcttatt
      121 atgaagcaat atcgaaagca ttgtttggat atataggtaa taaattatca attccaatga
      181 ctgattttac tgttgaaaca atagcagata cattgcataa aaaacaagtg ccggaaatta
      241 ttatacaaac ttgtattgaa atagtaaatc aatgcgaaat ggcacgctat gctccaattc
      301 aaagtataga agataaagaa caattattta ctaagacatc acaagtaata caaggaattg
      361 aacaaaatgt ataaacgtat gaaaactctc tatagtatag tgttcgtaat actaagtatt
      421 acgtttgtta atgcaaataa tcaattgaat gacagtataa ttcaaaatgc tcaaagatta
      481 tataaagctc aacaatatga tagtgtaatt gttttgtata agtctcttat ttctcaagga
      541 ttggtaagcg ataaattgta ttttaatctt gcaaatgcgt actataaaca aagagaaata
      601 gcacaagcaa tactttggta tgaaagagca tatgtattga atccctacaa cgaagatatt
      661 aaaaataatt tagctatagt tcaagcatta caacccgata aagttgaaca aatacaacaa
      721 agtatgttac agttgtggta tccttatgtg tatcgttttt gtaattccaa tacatgggca
      781 gtattgagtt tagtgttttt tatagggttt atagtatgtg tcttatggtt tttatttact
      841 aagcaaagag tgtataaaaa agtatcattt tttatagctt taggtagttt gggatttgca
      901 attattacat atatcaatgc gtcgtatcgt tacaatgaac ttgtaaaaaa tcaatatgct
      961 attgtaatgg aatcgagttt gagaatactt accgaaccta atgaaaatgc aaaagaatta
     1021 gttgttgtgc atggtggatt acgtgtgaaa atagaaaaga aaaatggaga ttgggtaagt
     1081 attcgtttgt cagatggtaa agttggttgg gttcaacatt ttgccataga aaaaatttaa
     1141 ttttaatatc atttttttta ttgtattttt tatagaataa ttggtattgc aaaccaataa
     1201 aaatgtattt ttacttcttg aaatttaaag cagtgtatga gggttgataa agaaaaaata
     1261 attgcaattc tgcaagaata ttggggcaaa actaagtact attcaaaaat attatggact
     1321 ttttatagag caaaagataa gaaatggtat gaaaaaatac ttcattgggg agcttttgtt
     1381 ttttcaatta tactcgtata cattattgct gtcgaaatta attttttgtg gttgtttgga
     1441 tatacaccta atttaagtga aataaataat cctaatcaat atgtagcttc agaagtgtat
     1501 gcatcaaatg gtaaattgtt aggtaagttt ttttacgaaa atcgtacacc ggttgaatac
     1561 aaagacttat caccattact tattaaaacg cttattgcta cagaagatgc tcgtttttat
     1621 caacatagtg gaatagattt tagagcattg gctccggtta taatggatgc tttaaaaggt
     1681 aatcctaggg gaggaagcac gctaacacaa caattagtta aaaatttatt taaaactcga
     1741 aatagaaatc atggtttatt aggtaaaata ccatttctta aaatttttat aataaaatca
     1801 aaagaatgga taggtgcttt aaaaattgaa ttgttctttt caaaagaaga tatactaaca
     1861 tcatatttga atacggtaga ttttggtagc aatgcctatg ggataaaaac ggctgccaaa
     1921 acgtatttta atactacacc aatgcgttta caacccgaac aatgtgctgt tttggtaggt
     1981 atgcttaaag caccaacggc atatagccct attttgaata aagaacgttc attatcgaga
     2041 cgaaatgtag tgttgggtat aatgaaacgt gacaatataa ttacagaaag agaatatgaa
     2101 gatttaataa aaaaacctat agtgttgcat tataatgtag aaaaaaatgt acatggtatt
     2161 gcaaattatt ttagaagggc tgtttttgat tatttaaaac cgtggctcaa agaaaacgat
     2221 ttggatattt atgccgatgg tttaaaaatt tacacaacac ttgattataa tatgcaggta
     2281 tatgctgaag aagctgtagc tcaaaatatg aaacgtgttc aacgtcaatt tgatgcgcat
     2341 tggcaagggc aaaatc
//
LOCUS       DAOTOW010000342         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35460_length_2349_cov_2.654752, whole genome shotgun sequence.
ACCESSION   DAOTOW010000342 DAOTOW010000000
VERSION     DAOTOW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35460_length_2349_cov_2.654752"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="P5243_06710"
     CDS             complement(<1..424)
                     /locus_tag="P5243_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRS19175.1"
                     /translation="MKRIFTVYLFILSMIVAQANVVLPSIFADHMVLQQNSEISVWGW
                     GKPYEQISVVGSWNGDTVKTVANNQANWKVTLKTGKAGGPYTLTVKGYTTIVIKDVLL
                     GEVWVCSGQSNMEWSARLGIDNAKQEVANANYPNIRFFT"
     gene            complement(476..1210)
                     /gene="pflA"
                     /locus_tag="P5243_06715"
     CDS             complement(476..1210)
                     /gene="pflA"
                     /locus_tag="P5243_06715"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRS19176.1"
                     /translation="MKTIRVHSIESFGTHDGPGIRMVVFLQGCNLQCIYCHNADTIPL
                     QSGTDMTSDEIVSQAIRLKPYFGTHGGVTFSGGEPLLQAKQLIPLIEQLHVHNIHVAI
                     DTNGTVKNVYSHNIMKQHADLVLFDIKAIDAEHFKSMTSNTLFEAHTEFIRIREQACK
                     PYWIRYVVVPGYTDAPHHIEALCNFVQPLKYLEKLQLLPYHSHGKHKWELMNMQYTLH
                     DVEAPCEAYMQKLSNILNKFCRVEIG"
     gene            complement(1226..>2349)
                     /locus_tag="P5243_06720"
     CDS             complement(1226..>2349)
                     /locus_tag="P5243_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS19177.1"
                     /translation="AFREYCAKVSIETSSIQYENDDVMRPSTECDDYGIACCVSLQEL
                     GKQIQFFGARTNLAKTLLLALNSGRCEYTGTQMVHEIPYIDSQYLDYDVVMKNFKTAM
                     HEVARVYNEAMNVIHYMHDKYYYEKAQMALIDTNPKINIVYGIAGLSIVADSLSAIKY
                     AKVKPIRNEDGLTVDFKIEGDFPKYGNDDDRVDSIACQVVQMFNEELKKLPVYKNATP
                     TMSVLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDTKGLIASLNSVAKIDYE
                     HAKDGISNTVSMIPQSLGSTLEQRITNLVQLLDGYFSQHAQHLNVNVLDKQTLLDAMD
                     YPELYPQLTIRVSGYAVNFVRLTREQQLEVITRSFHKHL"
BASE COUNT          702 a          459 c          412 g          776 t
ORIGIN      
        1 cagtaaaaaa tcgaatattt ggataatttg catttgcaac ttcctgtttt gcattgtcaa
       61 tacctaaacg agcactccac tccatatttg actgacctga acacacccat acttcaccaa
      121 gcaacacatc ttttataact atagttgtat aaccctttac cgttagggta tatggcccac
      181 ctgctttacc tgttttaaga gttactttcc aatttgcttg attatttgca acggttttta
      241 ccgtatcgcc gttccaactg cctacaaccg aaatttgttc atacggtttt ccccaccccc
      301 acacggaaat ttcagaattt tgttggagca ccatgtggtc tgcaaaaatt gaaggtaaca
      361 ctacattagc ctgagctact atcatactta gaataaataa atatactgta aatatccgtt
      421 tcataattct gtatttatta actgacatta aataaagaaa cacaaatata gttttttatc
      481 caatttctac cctacaaaat ttatttaaaa tgttacttag tttttgcata taggcctcac
      541 agggagcttc tacatcatga agtgtatact gcatattcat aagctcccat ttatgtttgc
      601 catgactatg gtagggtaat aattgtagtt tttctagata tttgagcggt tgtacaaaat
      661 tacacaatgc ttctatatgg tgtggagcat cggtatagcc aggaactacc acatagcgaa
      721 tccaatatgg tttacaagct tgttcgcgta tgcgaataaa ttctgtgtga gcttcaaata
      781 aagtgtttga tgtcatcgat ttgaaatgtt ctgcatctat tgctttgatg tcgaacaaca
      841 ctaaatcggc atgttgtttc ataatgttgt gtgaatatac atttttaaca gtaccattgg
      901 tgtcaattgc aacatgaata ttgtgaacat gtaattgttc tatgagtggt atcaattgtt
      961 tggcttgcaa caatggttct ccacccgaaa aagttactcc accgtgtgta ccaaaatatg
     1021 gtttcaatcg aatggcttgc gatacaattt cgtcgcttgt catatctgtg ccactttgga
     1081 gaggaatagt gtcggcattg tgacagtata tacattgtag attacaacct tgcaaaaaca
     1141 ctaccatgcg tattccgggg ccatcgtgtg tgccaaacga ttctattgaa tgaacgcgta
     1201 tagttttcat acgaaacgct atacgctata aatgcttgtg gaatgaacgg gtaataacct
     1261 cgagttgttg ttcgcgagta agacgaacaa aatttacagc atatcccgat acacgaatag
     1321 ttaattgtgg atataattcc ggataatcca ttgcatcgag cagtgtttgc ttgtcgagta
     1381 catttacatt aagatgttga gcatgttgac taaaatatcc atcgagtaat tgtaccaaat
     1441 tagtgatacg ttgttctaac gtagaaccca atgactgcgg aatcatcgac accgtattag
     1501 aaataccgtc ttttgcatgt tcataatcaa tttttgcaac agaattcagt gaagctatca
     1561 aacctttagt atcacgcccg tgcataggat tggcaccggg agcaaatgct acaccttttg
     1621 ctcttccatc gggagtagca ccggtttttt taccgtacat tacattagac gtaatggtaa
     1681 gcactgacat agtaggcgta gcatttttat atacaggtaa ttttttcaat tcttcattaa
     1741 acatttgcac cacttggcaa gcaatagaat ctactctatc atcatcattt ccatattttg
     1801 ggaaatcacc ttcaattttg aaatctacgg taagtccgtc ttcgttgcga ataggtttta
     1861 ctttggcata tttaattgct gaaagagaat cggcaactat agacaagcct gcaataccat
     1921 atacaatatt aatcttagga tttgtgtcaa tcaatgccat ttgagccttt tcgtagtaat
     1981 acttatcgtg catataatgt attacattca tggcttcgtt gtatacgcga gccacttcgt
     2041 gcatagcagt tttaaaattc ttcattacta catcgtagtc taaatattga ctatcaatat
     2101 atggaatttc atgtaccatt tgagtacctg tatattcaca acggccactg ttgagagcaa
     2161 gcaacaatgt ttttgcaagg ttagttcgag ctccaaaaaa ttgaatttgt ttgcccagtt
     2221 cttgcaacga cacacagcac gcaataccat aatcatcaca ttcagtagag ggacgcatta
     2281 catcgtcgtt ttcgtattga attgatgacg tttctattga taccttagca caatattcgc
     2341 gaaaagcct
//
LOCUS       DAOTOW010000343         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35498_length_2347_cov_3.535340, whole genome shotgun sequence.
ACCESSION   DAOTOW010000343 DAOTOW010000000
VERSION     DAOTOW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35498_length_2347_cov_3.535340"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /gene="trmB"
                     /locus_tag="P5243_06725"
     CDS             complement(<1..600)
                     /gene="trmB"
                     /locus_tag="P5243_06725"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRS19178.1"
                     /translation="MGKKDKLRRFAENETFDHVIQPQFNEVFNTNYKYKHKWSELYWK
                     NPNPIILELGCGKGEYTIQLAQKYPNCNCIGIDIKGARLWRGAKTALEDSLQNVAFIR
                     SRIEFLESFFGTDEISEIWITFPDPQKEKRRTKKRLTSPRFLNMYKNILQPHGKIHLK
                     TDSKELYEYTKSLLEFNKQPILIATDNLYESTVTNEILEI"
     gene            complement(605..1111)
                     /locus_tag="P5243_06730"
     CDS             complement(605..1111)
                     /locus_tag="P5243_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRS19179.1"
                     /translation="MVKNLRNQIKTVPKFVVVGIIITILGCYACNSKQVYNEKTSIDN
                     ATWSKDSPITFSYTVPDTITQYTLQVTVENTNDYMYSNLFLFSTITFPDSLTIRDTLE
                     CMLANYKGEWTGKGWWGYSNEFNFKHNIRFPKPGTYTFSFEQAMRCTNKSCELTGIKS
                     IGFRIIRL"
     gene            complement(1083..2342)
                     /gene="ricT"
                     /locus_tag="P5243_06735"
     CDS             complement(1083..2342)
                     /gene="ricT"
                     /locus_tag="P5243_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRS19180.1"
                     /translation="MKSSSKRSGSSKLYCYNWLQDIKHIPATHNLVEIRFKNTRKDFY
                     ENNLKLSLEVGDIVAVEASPGHDIGIVSLTGELVLEQLRKVYGTKVPETFPKIYRKAK
                     PNDIEKWEEAKALEHKTMIKSRQIAKDLKLQMKIGDVEYQGDKTKAIFYYIADERVDF
                     RELIKILAEQFRIRVEMRQIGARQEAGRIGGIGPCGRDLCCSSFVSNFVSVSTTSARY
                     QEISLNPQKLAGQCGKLKCCLNYELASYMDARKDFPDTTIALEFSDVKATHAKTDVYK
                     RIMWYFFKKDEIPHFLELTVDQIHEIIALNKKGIKPERPEQIASQSKAKVDVSFDYTN
                     VVGQESLTRFDKQNNQKNRPNQNKQKNQGNNQQQKQTQHNQSEQVQNKQGNTQNKQQN
                     NNKQQFNKHKQKPNQQNNNGQKPQESN"
BASE COUNT          647 a          357 c          406 g          937 t
ORIGIN      
        1 tatttctaag atttcgtttg taacagtaga ttcatataaa ttgtcggtag caatgagtat
       61 gggttgttta ttgaattcta atagactttt agtgtattca tataattctt tactgtcggt
      121 ttttaaatga attttgccat gtggttgcag aatatttttg tacatattca aaaaacgggg
      181 agaggttaac cgtttttttg tgcgtcgttt ttctttttgc ggatcgggaa atgtaatcca
      241 aatctctgat atttcatcag ttccaaaaaa tgattcaaga aattcaatac gcgaacgtat
      301 aaatgctacg ttttgtaaag aatcttctaa tgcagttttt gcaccacgcc aaagtcttgc
      361 accttttata tctatgccaa tacagttaca gtttgggtat ttttgagcaa gttgtattgt
      421 gtattctcct ttgccgcatc ctagttcaag aattattgga ttaggatttt tccaatacaa
      481 ttcactccat ttgtgtttgt atttgtaatt agtattaaaa acttcattga attgtggttg
      541 aattacatga tcaaatgttt cgttttcggc aaaacggcgt aatttatctt ttttacccat
      601 gtgattataa tctgattatt cgaaacccaa ttgattttat accggttaat tcacatgatt
      661 tgtttgtgca tcgcatagct tgttcaaaac tgaacgtgta tgtacccggt ttggggaagc
      721 gaatattatg tttgaaatta aattcatttg aatatcccca ccaacctttt cccgtccatt
      781 ctcctttata atttgcaagc atacattcca gtgtgtcgcg aatggtaaga gaatcaggaa
      841 aggtaattgt tgaaaataaa aagagatttg aatacatata atcgttcgta ttttcaacag
      901 taacttgtaa tgtatattga gttatagtat caggaactgt atatgaaaat gtaataggag
      961 agtctttgct ccatgtggca ttatcaatag aggttttttc attgtacact tgttttgaat
     1021 tgcaagcgta acatcctaat atagttataa taataccaac aacaacaaat tttggtactg
     1081 ttttaatttg attcctgagg tttttgacca ttattattct gttgatttgg tttttgcttg
     1141 tgtttattaa attgttgttt gttattgttc tgttgtttgt tttgagtatt tccttgttta
     1201 ttttgtactt gttcagattg attgtgttgt gtttgttttt gttgttgatt gttaccttga
     1261 tttttttgtt tgttttggtt tggacgattt ttttgattat tttgtttgtc aaatcgagtt
     1321 aggctttctt gaccaactac attggtataa tcaaaagaaa catcaacttt tgcttttgat
     1381 tgagaggcaa tttgttcagg acgttccggt tttatgcctt ttttgttaag agctataatt
     1441 tcatgaattt gatcaaccgt taattctaag aaatgaggta tttcatcttt tttgaaaaaa
     1501 taccacatta ttcgtttata tacatcggtt tttgcatgag tagcttttac atctgaaaat
     1561 tcaagtgcta tggtagtatc aggaaaatct ttgcgggcat ccatataact tgcaagttca
     1621 tagttaagac agcattttaa ttttccacat tgtccggcaa gtttttgagg atttaaagaa
     1681 atttcttgat aacgagccga agtagtagaa accgatacaa aattagatac aaaagaagaa
     1741 caacataaat cgcgaccgca aggaccaata ccaccaattc tacctgcttc ttgtcgtgct
     1801 cctatttgtc tcatttcaac tcgtatacga aactgttcgg caagaatttt tatgagttct
     1861 ctaaagtcaa cacgttcatc ggcaatataa tagaatatgg ctttggtttt atctccttgg
     1921 tattctacat cgcctatttt catttgaagt tttaaatctt tggcaatttg tctcgattta
     1981 atcattgttt tatgttccaa agctttggct tcttcccatt tttctatatc atttggcttt
     2041 gcttttctgt aaattttagg aaacgtttcg gggacttttg tgccatatac tttgcgtagt
     2101 tgttcaagta ctaattcccc cgtaagcgaa actattccta tatcatgtcc gggagaagct
     2161 tctacggcca ctatatcgcc aacttctaaa gacagtttta agttattttc ataaaaatct
     2221 ttacgagtgt ttttaaatcg tatttcaacc aaattgtgtg ttgcaggtat atgtttaatg
     2281 tcttgtaacc aattgtaaca gtatagttta gaactgccac tgcgttttga tgatgatttt
     2341 atttttt
//
LOCUS       DAOTOW010000344         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35707_length_2336_cov_3.697501, whole genome shotgun sequence.
ACCESSION   DAOTOW010000344 DAOTOW010000000
VERSION     DAOTOW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35707_length_2336_cov_3.697501"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="P5243_06740"
     CDS             complement(<1..870)
                     /locus_tag="P5243_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRS19181.1"
                     /translation="MNNNFFHKELLNKYLNYLEFEKRSSKHTVASYKRDILDFFETTQ
                     DSKNIENILDSDIRFWIVKLSEKEVTARSINRKITAVRSFFEYLLKFGYVSKNPAVKI
                     HALKTKKRLPIFVEKQSMSNLFELVTFEEGFVGLRDKIILDLLYSTGMRRAELLTLQT
                     SSFNYGNKTVKILGKRNKERIVPLVPELIKSIQVYLQERAKIAQSHESFIITDKGAKA
                     YEQLIYRVVKKYLGQITTLEKKSPHVLRHTCATHLLNNGADLHDIKELLGHANLSATQ
                     IYTHNSFEKLKDIY"
     gene            complement(860..1054)
                     /gene="rpsU"
                     /locus_tag="P5243_06745"
     CDS             complement(860..1054)
                     /gene="rpsU"
                     /locus_tag="P5243_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRS19182.1"
                     /translation="MIIVPIKEGENIDRALKKFKRKFEKTGIVKELRNRQAFTKPSIK
                     RREQIIKAVYKQKLQLTDEQ"
     gene            complement(1140..1967)
                     /locus_tag="P5243_06750"
     CDS             complement(1140..1967)
                     /locus_tag="P5243_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRS19183.1"
                     /translation="MISRRFSFNLLSLVVVLTIALCVYLFIPHKQSIHYNNLPNDSII
                     LYTNTNEVLEKISKKINPNTATFKELCEAGFTKKQATNCINYIQAGNTFSYIEDIRKL
                     YTIQDSDFKRISKNLYIPPHKYIVEQKQINTPNTIIQKKTKLQVNLNTSDSLELQKLP
                     GIGTFRAKKIIEMRTKLGGFYTIHQLQSIYSFDSILIEKIRPFCYIDSSQIQKINLNS
                     TTFKDLVAHPYVSYYIAKNILDYKKIVGTIESIEELRTNAIIKPKDYEILKYYIKTF"
     gene            complement(1964..2242)
                     /locus_tag="P5243_06755"
     CDS             complement(1964..2242)
                     /locus_tag="P5243_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRS19184.1"
                     /translation="MKGFDFLFGSFCEKYFNNRSYAEKAYKILFLVFIIVGTASSNSN
                     VIDFSDMMLLCMAFPNMIGLVVFAPRLQKDLQKYWKQITNKTVSQATK"
BASE COUNT          722 a          314 c          322 g          978 t
ORIGIN      
        1 ataaatatct tttaattttt caaacgaatt gtgggtatat atttgtgttg ccgataaatt
       61 tgcatgccca agtaactctt ttatgtcatg taagtctgct ccgttattta ataaatgtgt
      121 tgcacatgtg tgtcgtaata catgtggact ttttttctct aaagtagtta tttgtcccaa
      181 atattttttt acaactcggt atattaactg ttcgtaagct tttgctcctt tgtctgttat
      241 tataaaggat tcatgtgatt gtgctatttt tgcacgttct tgtaaatata cttgaatact
      301 ttttattaat tcaggtacta aaggcacaat tcgttcttta ttcctttttc ccagtatttt
      361 taccgttttg ttcccataat tgaatgaact tgtttgtaaa gttagcaatt ccgctcgccg
      421 catacctgtg gagtacaata aatctaaaat tattttatca cgtaatccaa caaatccttc
      481 ttcaaaagtt actaactcaa atagattact catagattgt ttttcaacaa atatcggtaa
      541 tctttttttt gtttttaatg catgtatttt tactgccgga ttttttgata cataaccaaa
      601 tttgagtaaa tattcaaaaa atgaccgaac ggcagtaatt tttctgttta tacttcgtgc
      661 tgttacttct ttttctgata atttaactat ccaaaatcga atatcagaat ctagaatatt
      721 ttctatattt tttgaatctt gtgtggtttc aaaaaaatct agaatatctc ttttatacga
      781 cgcaacagta tgttttgaac tccttttttc gaattctaag tagttcaaat acttgtttaa
      841 taattcctta tgaaagaaat tattgttcat ctgttaattg taatttttgt ttataaacag
      901 cttttattat ttgttctctt cttttgattg atggttttgt aaatgcttga cggtttctca
      961 attcttttac aataccggtt ttttcgaatt tacgtttaaa tttttttaac gctctgtcga
     1021 tgttttcacc ttctttgata ggtacaataa tcatattttt actcctttac tgttaaataa
     1081 tttttttaaa aatgggttgc aaatgtataa acttcggttt gaatgaacaa atttttttac
     1141 taaaatgttt taatatagta ttttaatatt tcataatctt tgggttttat tattgcattt
     1201 gttcttaatt cttcaatgct ttcaattgtt ccaactattt ttttataatc taaaatattt
     1261 tttgcaatat aatatgaaac atagggatgt gctactaaat ctttaaaagt tgttgaatta
     1321 agatttattt tttgtatctg agaactgtca atgtaacaaa atggtcttat tttttctata
     1381 agtatagaat caaagctata aattgattgt aattgatgaa tagtataaaa accacctaat
     1441 tttgttcgca tttcaataat tttttttgct ctaaatgtac ctatgcccgg taatttttgt
     1501 aattcaagag aatcactagt gtttaagttt acttgtaatt ttgttttctt ttgaataata
     1561 gtatttggtg tatttatttg tttttgttca actatatatt tgtgaggagg tatatacaag
     1621 ttctttgaaa tcctcttaaa atcggaatct tgaattgtat ataattttct tatgtcttca
     1681 atgtatgaaa atgtatttcc ggcttgtata tagtttatac aattagttgc ttgttttttt
     1741 gtaaagccgg cttcacataa ttctttgaat gtagcggtat tgggatttat ttttttactt
     1801 attttctcta atacctcatt tgtattagtg tataaaataa tagaatcatt aggcaggttg
     1861 ttgtagtgaa tactttgctt atgaggtata aataagtata cacataatgc tatagttagt
     1921 acaaccacaa gagataatag attaaacgag aagcgtcttg aaatcattta gtagcttgac
     1981 taacagtttt attcgtaatt tgtttccaat atttctgtaa atctttttgg agtcgaggag
     2041 caaataccac taaacctatc atattaggga atgccataca aagtaacatc atatcagaaa
     2101 aatcaattac gttggaattg ctcgaagctg tacctacaat aataaatact aaaaataaaa
     2161 ttttatatgc tttttcggca tacgaacgat tgttgaaata tttctcgcaa aatgaaccaa
     2221 ataaaaaatc aaatccttta attccataat atgaccatga tataagtgta gaatatgcaa
     2281 atagttgtca agtttcacaa ggttgtttat gataacttaa ttgcaaagat aaaact
//
LOCUS       DAOTOW010000345         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35712_length_2336_cov_2.750548, whole genome shotgun sequence.
ACCESSION   DAOTOW010000345 DAOTOW010000000
VERSION     DAOTOW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35712_length_2336_cov_2.750548"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2290
                     /locus_tag="P5243_06760"
     CDS             <1..2290
                     /locus_tag="P5243_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19185.1"
                     /translation="VFINFDNAVCQSLRINNNVEGTASVFFSDISSKLLVNGVVTLSG
                     NTINCNGTLSMANGGYLECNSISLGGGNAVFSPGTGTLQMNAVNSFPLSVVNNLIIRN
                     GLTTLTRNLIIQGDLTIDLGPGYAGQIDVTTNNYSVSIAGNWFHKNPSDGFIERLGTV
                     TFNGVGNQIFTSYGLYNNVVVNKPSGVVLFRGWVAPATVNNILEIYGNLTITSGVLRI
                     DENATRLSIRGNLNISGELDMSKSDITNPGNRTVLGGILTLSANGILRIGGTNTFPAN
                     YSTISLSPTSTVEYCGTNQTVRGGITYGNLHITNSGTKTLASNCNVAGNIIVNQGNFN
                     TGVRRITCVGSLNQTFFGVSLYDITIQKSNAGVLLSTETSVSNNITLTQGIVDATQAS
                     LVLLENATVLGASNISHVMGVVKKITNSLTEFIFPIGDGQFYRPISIVNPSNNIWSAQ
                     YFFSGYGNTFTVNPTDNPKLHHVSDIEYWDISPQLPGGSSKIKLTWNINSDVLYPEYL
                     LIAHWNSNQNYWENVGDVIVDEVSQTITSVLPFSSFSPFTFGSTHEENPLPIELIRID
                     GYCDNFNKIHISWTTASEKNNELFTVYKSSNGILWHEIGTLKGAGNSNEQNTYSFIDS
                     EKVDYFAYYKIIQTDFGGTKQELDLMCVFCQKVSHDISLIIHPLENELELCFFNFNDT
                     VEPLNVYIYNQQGKLIDVNNFTAIPNYNEQRISLSEINAEGVYIIQIKRNNSIVYSAS
                     FLKKKSVESKNRYIAEIQKIGF"
BASE COUNT          818 a          285 c          412 g          821 t
ORIGIN      
        1 cgtatttatt aattttgata atgctgtttg tcaaagttta cgaataaata ataatgtaga
       61 agggacggca tctgtttttt tttctgatat ttcttcaaag ttacttgtta atggggttgt
      121 tactttaagt ggaaatacaa ttaactgtaa tggtacatta tctatggcaa atggaggata
      181 ccttgagtgt aatagtataa gtttaggtgg aggcaatgct gtattttctc cgggaacagg
      241 tactttacaa atgaatgctg taaatagttt tccattatct gtagttaata atttgattat
      301 tcgaaatgga ttaacaactc ttacacgaaa tttgattatt cagggagatt taacaattga
      361 tttgggacca ggttacgcag gtcaaattga tgttactaca aataattatt ctgtgagtat
      421 tgcaggaaat tggtttcata aaaatccgtc tgatggtttt attgaacgat tgggaacagt
      481 tacgtttaat ggcgttggta atcaaatttt tacttcgtat gggttatata ataacgttgt
      541 tgtaaataaa ccatcgggag ttgttttgtt tcgtggttgg gtagcaccgg ctaccgtgaa
      601 taacatacta gagatttatg gtaatttaac gataacttca ggagtactcc gtatcgacga
      661 aaatgccaca cgtttaagta tacgaggaaa tttaaatatt tctggagaat tagatatgtc
      721 gaaaagtgat attacaaatc cgggaaatag aactgtactt ggtggaatct taactttaag
      781 tgcaaatggt attttaagaa taggtggtac taatactttt cccgcaaatt attctactat
      841 ttcattatca ccaacatcaa ctgttgaata ttgtggaaca aatcaaactg ttaggggagg
      901 tattacctat ggaaatttac acataacaaa ttcaggaaca aaaacattag catcaaattg
      961 taatgttgcc ggaaatatta ttgttaatca aggaaatttt aatacaggtg ttcgcagaat
     1021 tacatgtgtt ggatcgttaa atcaaacatt ttttggtgtt tcattgtatg atattactat
     1081 tcaaaaatca aatgcaggag ttttattatc tacagaaact tcagtttcaa ataatattac
     1141 tttaactcaa ggtatagtag atgcgacaca ggcttctctt gtattattag aaaatgcaac
     1201 tgttttgggg gcttctaata taagtcatgt tatgggagtt gtgaaaaaga ttacaaattc
     1261 attaaccgaa tttatatttc ctattggaga tgggcaattc tatagaccaa taagtattgt
     1321 aaatccaagt aataatatat ggtcggcaca gtattttttt tcgggatatg gtaatacatt
     1381 tactgtaaat cctactgata atcctaaatt acaccatgtg tctgatattg aatattggga
     1441 tatttcacca caattgcccg gtggttcaag caaaataaag ttaacatgga atataaatag
     1501 tgatgtgtta tatcctgaat atttacttat agctcactgg aattctaatc aaaattattg
     1561 ggaaaatgta ggagatgtta ttgtagatga agtatcacaa acaataactt ctgtgctccc
     1621 tttttcaagt tttagtccat ttacatttgg ttctacacac gaagaaaacc cattgcctat
     1681 tgaattaata agaattgatg gatattgtga taattttaat aaaatacata tttcatggac
     1741 tacagcatct gaaaaaaaca atgaattatt tactgtttat aaaagttcaa atggtattct
     1801 atggcatgaa attgggacat tgaaaggtgc cggtaattca aatgagcaaa acacgtatag
     1861 ttttattgat tcagaaaagg tagattattt tgcttattat aaaattattc aaactgattt
     1921 tggaggaacg aaacaagaac ttgatcttat gtgtgtgttt tgtcaaaagg tgtctcatga
     1981 tatttcttta attattcacc ctttagaaaa tgaattagaa ttatgttttt ttaattttaa
     2041 tgatactgta gaacctttga atgtatatat ttataaccaa caaggaaaat tgatagatgt
     2101 aaataatttt acggccatac ccaattacaa tgaacagaga atatctttat cagaaataaa
     2161 tgctgaagga gtatatataa ttcaaataaa aagaaacaat tcaatagtgt attctgcatc
     2221 gtttttgaaa aaaaaatcag ttgaaagtaa aaatagatat attgctgaaa ttcagaaaat
     2281 tggattttaa tgagtgttgt ttttttttta ataaaaaata tagattacaa ataaaa
//
LOCUS       DAOTOW010000346         2332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35779_length_2332_cov_3.689504, whole genome shotgun sequence.
ACCESSION   DAOTOW010000346 DAOTOW010000000
VERSION     DAOTOW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35779_length_2332_cov_3.689504"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..507)
                     /locus_tag="P5243_06765"
     CDS             complement(16..507)
                     /locus_tag="P5243_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRS19186.1"
                     /translation="MKLVSTIIIACVFFSCKGQKPKVDNSVVSLLDITKYVGTWYEIA
                     RYDHTFERGLVGVTATYTLLEDGFIQVVNKGFKDSLTGKEKVARGKAYIPNIETPSKL
                     KVSFFLFFYSDYYVLELDKQYQWAVVGSKSDAYLWILYREPHMNSELYDCKLPQKPDS
                     LKV"
     gene            complement(561..1922)
                     /locus_tag="P5243_06770"
     CDS             complement(561..1922)
                     /locus_tag="P5243_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRS19187.1"
                     /translation="MENEVLLAQLSNMIKENCRLDSELFDKFDVKRGLRNKDHSGVLV
                     GLTNIGNVVGYKKNEVTGKLDPIPGELRYRDIEVTDLVNGCSASKMHGFEEIVYLLLA
                     GKLPNRAELAAFSSYLADNRDLTSSFTKNMILSLRGKDIMNMLARSVLALYTLDKHAD
                     DTTPENLVKQSLKLIAQVPVIIAYSYHSMMNAYYRKELIIRHPNPELSTAENFLYLIK
                     GEGNYTKTEADILDLSLILHADHGGGNNSTFTIRVTSSSGTDTYAAVASAIASLKGPL
                     HGGANLQVEEMMDDIRKNVRNWEDEQEIYDYLIKILKKEAFNKSGKIYGMGHAVYTIS
                     DPRAILLRERAAVLAKEKGLEKEFNLYTKVEKLAQKAFYDFKTGTDKTVCANVDFYSG
                     FVYNAIGLPKELFTPIFAMSRMAGWCAHRIEELNFDQRRIIRPAYKAVYTGPGYIPID
                     ERK"
     gene            complement(1936..>2332)
                     /locus_tag="P5243_06775"
     CDS             complement(1936..>2332)
                     /locus_tag="P5243_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRS19188.1"
                     /translation="KQKGLASIVVAEENYGEGSSREHAAMEPRFLGVKVILAKSFARI
                     HETNLKKQGMLALTFVNTADYDLILEGDIISVIGLTSFSEGKNLIIELIHKDGSKHSF
                     EAKHTYNSVQIEWFKAGSALNLIAKESNK"
BASE COUNT          764 a          434 c          305 g          829 t
ORIGIN      
        1 aaattaatac tttttttata cttttaaact gtccggtttt tgggggagct tacaatcata
       61 taattccgaa ttcatatgtg gttctctata taaaatccac aaatacgcat cggatttact
      121 ccctactaca gcccattgat attgtttgtc taattcaagc acataataat ctgaataaaa
      181 aaataaaaag aaagaaactt ttagtttaga aggtgtttca atatttggaa tataagcctt
      241 acctcttgca actttttctt taccggtaag agaatcttta aaccctttat taactacttg
      301 aataaatcca tcttctaata acgtatatgt agcagtaact ccaaccaaac ctcgttcaaa
      361 tgtatggtca taccgagcaa tttcatacca cgtacccaca tattttgtta tatctaacaa
      421 agaaacaaca gaattatcaa ctttaggttt ttgaccttta caagaaaaga acacacatgc
      481 aataataata gttgaaacta atttcataat attacaattt ttaataaaaa aaggggcata
      541 tgtttatgcc cctttgtatt ttattttctt tcatcaattg gaatatatcc tggtccggta
      601 tacacagctt tatatgccgg acgaataatt cttcgttggt caaaattaag ttcttcaatt
      661 ctatgagcac accaaccggc catacgagac atggcaaata tgggtgtaaa taattctttt
      721 ggcaacccaa tagcattata tacaaatccc gaataaaagt caacattggc acagactgtt
      781 ttatcggtac cggttttgaa atcataaaat gccttttgag caagtttttc aactttggta
      841 tataaattaa attccttttc taatcctttt tcttttgcaa gaacagctgc acgttcacga
      901 agtaaaattg cacgtgggtc agaaattgta tatacagcat gtcccattcc atatattttt
      961 cctgatttat taaatgcttc ttttttaagt attttaatca aataatcata tatttcttgt
     1021 tcatcttccc aatttctgac atttttacga atatcatcca tcatttcttc aacttgcaaa
     1081 ttagctcccc catgcaaagg tcctttcagt gacgctatag cagaagctac tgctgcatat
     1141 gtatcggtac ccgatgaact tgttacccta attgtaaatg tagaattatt acctccccca
     1201 tgatcagcat gtaaaataag acttaaatct aaaatatcag cctcagtttt agtatagtta
     1261 ccttctcctt ttataagata caaaaagttt tctgctgtag ataattcagg atttggatga
     1321 cgaataatta attctttacg ataatacgca ttcatcatac tatgatacga atatgcaata
     1381 ataacaggaa cttgtgcaat cagttttaat gattgtttta ctaaattttc aggagttgta
     1441 tcatcggcat gtttatctaa agtataaaga gctaataccg aacgagcaag catattcata
     1501 atatctttac cacgtaaaga aagtatcata tttttagtaa aagaacttgt aagatcacga
     1561 ttatctgcaa gatatgaact aaacgctgca agttctgccc tatttggtaa tttacctgct
     1621 aacaacaaat atactatttc ttcaaaacca tgcattttag aagcagaaca accatttacc
     1681 aaatctgtaa cttcaatatc tctatatcgt aattctccgg gaataggatc taatttaccg
     1741 gtgacctcat tttttttata cccaactacg ttacctatat tagtaagacc aaccaataca
     1801 ccagaatggt ctttatttct caatccacgt tttacatcaa acttatcaaa taattcactg
     1861 tcaagtctac agttttcttt tatcatattt gataattgag ctaataatac ttcattttcc
     1921 atatgtttct atgttttatt tgtttgattc ttttgctatt aaattaagtg cacttcctgc
     1981 tttaaaccat tctatttgca ccgaattata ggtatgtttt gcttcaaatg aatgttttga
     2041 accatcttta tgtataagtt ctattataag attttttcct tcagaaaaag aggttaaacc
     2101 tataacagaa attatatcgc cttccaatat taaatcataa tcggcagtat taacaaaggt
     2161 tagtgcaagc ataccttgtt tctttaaatt tgtttcgtga atgcgagcaa atgattttgc
     2221 taaaattact ttcacaccta aaaaacgagg ctccattgct gcatgttcac gcgatgatcc
     2281 ttctccataa ttttcttcgg ctaccacaat agaagctaac cctttttgtt ta
//
LOCUS       DAOTOW010000347         2331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35802_length_2331_cov_3.248243, whole genome shotgun sequence.
ACCESSION   DAOTOW010000347 DAOTOW010000000
VERSION     DAOTOW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35802_length_2331_cov_3.248243"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="P5243_06780"
     CDS             complement(<1..298)
                     /locus_tag="P5243_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIA DNA topoisomerase subunit B"
                     /protein_id="HRS19189.1"
                     /translation="MSVEYTEESIKSLDWKEHIRRRPGMYIGKLGDGSASDDGIYILL
                     KESLDNSIDEYMMGYGKTIDIQISEHTVAIRDYGRGIPLGKVLDVSSKINTGGKY"
     gene            620..1414
                     /locus_tag="P5243_06785"
     CDS             620..1414
                     /locus_tag="P5243_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS19190.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLGASLAVLEYKVLIIDFDPQGN
                     ATQGVGIDDKTISKGIYECIVGKIDPRTAILQTELEKLHILPANIDLVGAEIEMLNQR
                     DRELVLKQAIEPIRNDYDFILIDCSPSLGLLTVNALTAADSTIVTVQCEYFALEGLGK
                     LLNTIKIIQTRLNPALHIEGFLLTMYDIRLRLANQVVEELKTHFQSMVFETLIQRNVR
                     LGEAPSFGKPVILYDANSNGSINYLNFAKELLIKNNLPEIKEGVAV"
     gene            1411..2298
                     /locus_tag="P5243_06790"
     CDS             1411..2298
                     /locus_tag="P5243_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRS19191.1"
                     /translation="MTKKNALGKGIGALLGDSESIGKPVHRPPLKTGIELEISKIQAN
                     PNQPRTKFDEDALAELAESITHLGVIQPITVREVGPETYQIISGERRFRAAQLAGLDS
                     IPAYIRTVNEVDMLTMALVENVQREDLDAIEIATSYQRLIEECNLTQEKLSERVGKKR
                     ATIANYLRLLKLPPEIQLGIINEDISMGHARALITIEDTDLQVQLFTKIIESELSVRE
                     VEEMIRKYKEVKQIDDAVSAKQKLTEEYKLLQDDLCNRFKAKVSFSISPKGNGKITIP
                     FSSEVDLERILGILDKIKE"
BASE COUNT          860 a          376 c          365 g          730 t
ORIGIN      
        1 catatttgcc acccgtattt atttttgaac ttacatcaag aaccttcccc aaaggtattc
       61 ctctaccata gtcacgaata gctaccgtat gttcagaaat ttgaatatca atggttttac
      121 catatcccat catatattca tcaatagaat tatcaagaga ttcttttaat aaaatatata
      181 taccatcatc actggcagaa ccatcaccca atttacctat atacattccc ggacgacgac
      241 gaatatgctc tttccaatca aggctcttta tactttcttc ggtatattct actgacatag
      301 ttgcattgtt ttttgaacta tcaaaagtac acgttttaca actgtttttt ttgcattcat
      361 acaagataat attatcaaat tattaacaga aaaatgcatg taaaaaaaac aaactgtact
      421 atttttttta aatacacttc taaaaaatat agcacaagac acaaaaaaaa ctgcatcgct
      481 gttaataact ttttatcaat taaaaatcaa gacattagaa gcaaattgat gaataaaaat
      541 gtgatatcaa aatatatgat aatttttaga acttgtattt tttgtatttt tgaccattca
      601 agccataaat aataataaaa tgggtaaagt aatagcaata gcaaatcaaa aaggtggtgt
      661 aggtaaaact actactgcaa taaatttggg tgcaagttta gcagtactcg aatacaaagt
      721 gttaattatt gattttgacc ctcaaggtaa tgcaacacag ggagttggta tagatgacaa
      781 aactatatca aaaggcatat atgaatgcat agttggcaaa attgatccac gcacagctat
      841 attacaaacc gaattagaaa aactacacat tttacctgct aacattgact tagttggtgc
      901 tgaaatagaa atgcttaatc aaagagatag agaacttgtg ttaaaacaag cgattgaacc
      961 tattcgtaat gattatgatt ttatacttat tgattgttct ccatcacttg gattacttac
     1021 ggtaaatgca cttacggcag ctgattcaac tattgtaaca gtacaatgcg aatattttgc
     1081 tcttgaagga ttaggcaaac tgcttaatac tataaaaata atacaaacac gattaaatcc
     1141 ggcattacat attgaaggat ttttgctaac tatgtacgat atacgacttc gtttagcaaa
     1201 tcaagttgtt gaagaactca aaactcattt tcaatcaatg gtatttgaaa ctcttattca
     1261 acgcaatgtt cgtttgggtg aggcacctag tttcggtaaa cctgtaattt tatacgatgc
     1321 taattctaac ggttcaatta attatcttaa ctttgccaaa gaattactta taaaaaataa
     1381 tttacctgaa ataaaagaag gcgtagcagt atgacaaaaa aaaatgcgtt aggcaaagga
     1441 ataggtgccc tattgggtga ttctgaaagc attggcaaac cggttcacag acctccgttg
     1501 aaaaccggaa tagaattaga aatatcaaaa atacaagcaa atcctaatca acctcgcaca
     1561 aaatttgatg aagatgcttt ggctgaactt gctgagtcta ttactcattt aggtgttatt
     1621 caaccaatta ccgttcgcga agttgggccc gaaacatatc aaataatttc cggagaacga
     1681 cgatttagag ctgctcaatt agcaggttta gatagtattc ctgcatacat aagaacggta
     1741 aatgaagttg atatgcttac tatggcattg gttgaaaatg ttcaacgtga agaccttgat
     1801 gctattgaaa ttgctaccag ctaccaacga ttaattgaag aatgcaattt aacacaagaa
     1861 aaacttagtg agcgtgtagg aaaaaaacgt gctactatag caaattattt acgacttctc
     1921 aaactacctc ccgaaattca attaggtatt ataaatgaag acatttctat ggggcatgct
     1981 cgtgctctta taacaataga ggatactgac ttacaagttc agttatttac taaaataatt
     2041 gaatctgagc tttctgttcg cgaagtagaa gaaatgattc gtaaatacaa agaagtaaaa
     2101 caaattgacg atgcagtttc ggctaaacaa aaattaaccg aagaatataa attattacaa
     2161 gatgatttat gtaaccgatt caaagctaaa gtatcttttt ctatttctcc caaaggtaat
     2221 ggcaaaataa caattccatt ttcttctgaa gttgatttag aaagaatatt aggtatttta
     2281 gataaaatta aagaataact gtatgtcaaa atatttggga attatttgtt t
//
LOCUS       DAOTOW010000348         2326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35899_length_2326_cov_3.363276, whole genome shotgun sequence.
ACCESSION   DAOTOW010000348 DAOTOW010000000
VERSION     DAOTOW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35899_length_2326_cov_3.363276"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="P5243_06795"
     CDS             <1..1014
                     /locus_tag="P5243_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS19192.1"
                     /translation="KPKNAGNVVNTAGDEVFTYLRNDSILYFSSNGHVGMGGLDIFRL
                     NRDAKGREYVLNLLPPVNSTFDDYGITFRQEGKEEGYFSSNRSKGSKGSDDIYHFALP
                     ILTYSISGKIINELTDSPIAYANVRLIGSDGSSLELFSDKDGNYAFSLNPQTNYVIIA
                     IHDGFLNSKYKISTYGLQKDKTFDVNLYMTQIDIPVEIPNIMYDVNRWELRPESIVAL
                     EKLLDILRDNPHIIIELSSHTDYRVGRISNEELSQLRAQSVVNFLIAKGISPNRLVAK
                     GYGATRPKKIDLKYSELYSFLKLGDVLTPEYIQSLPETYRETCHQINRRTEFRVISTE
                     YKN"
     gene            1020..1730
                     /locus_tag="P5243_06800"
     CDS             1020..1730
                     /locus_tag="P5243_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19193.1"
                     /translation="MKTYLLSVLLCIVASVTVYSQDIIILKTGDEIQSKVEEIRPDVV
                     TYRKFENLQGPLYTIEKSRIFMIKYANGSKDVFSDITVQQQQQIQQSNAIPKPTQPAI
                     TEANKEEPLQYIFGGTIRRNNTNLTPFKVKEIMRSNPEAFSLYSSGRSLKVTGDVFEG
                     IGYGCFVFMVINSLDNKEIQARRAAGFGIATMSTSLILEAIGKSNMRKAVNTYNAGLK
                     STSYLFLSPNSMGYCLRF"
     gene            complement(1727..>2326)
                     /locus_tag="P5243_06805"
     CDS             complement(1727..>2326)
                     /locus_tag="P5243_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRS19194.1"
                     /translation="IFYKFLYLAANFYYMAFLDIFLIALGLAMDSFAVSITAGLILKK
                     CKIKDVMPIAVYMGFFQGVMPIFGWLAGKELAQYIESFDHWIAFVLLVFIGGKMIYEN
                     AIKGNKHCFNPKKHIVLLGLAFATSIDALIIGVNFGLLRLELARPILIIGITTFILSF
                     AGVYLGGRFQKLYAVKMETIGGIVLIGIACKIVFEHIWC"
BASE COUNT          835 a          345 c          393 g          753 t
ORIGIN      
        1 aaaccaaaaa atgcaggaaa tgttgtaaac acagccggtg atgaagtgtt cacgtatctt
       61 agaaatgata gtatcttgta tttttcttca aatggacatg taggaatggg aggtcttgat
      121 atatttagat tgaatcgcga tgccaaaggt cgagaatatg ttttgaattt attacctcca
      181 gtaaattcta catttgatga ttatggtata acttttagac aagaaggaaa agaagaagga
      241 tatttttctt caaatcgttc aaaagggtct aaaggttcag atgatatata tcattttgct
      301 ttaccaatat taacgtattc tatttcagga aaaattatta atgaacttac cgattcgcct
      361 attgcctatg ccaatgttag gttaataggt agtgatggct catcattaga actattttct
      421 gacaaagatg gtaattatgc ttttagtctt aatccgcaaa caaattatgt tattattgca
      481 attcatgatg gttttttaaa ttcaaaatat aaaatatcaa cttacggatt acaaaaagat
      541 aaaacatttg atgtaaattt atatatgaca caaattgata ttcctgtaga aatacctaat
      601 ataatgtatg atgtaaatag atgggaatta agaccggagt caattgtagc attagaaaaa
      661 ttgcttgata tattacgcga caatccgcat ataattattg aattgagttc acataccgat
      721 tatagggtcg gtagaatttc aaatgaagaa ttgtcacagt tgcgagcaca gtctgttgta
      781 aattttttaa ttgctaaagg tataagtcca aatcgtttag tagctaaagg ctatggagca
      841 acacgaccta aaaaaattga tttaaaatat tcggaattat atagtttctt gaagttgggt
      901 gatgtgctta ctcccgaata tattcagagt ttgcccgaaa catatagaga aacgtgtcat
      961 caaataaaca gacgtaccga atttagagtt atttcaacag aatataaaaa ctaacgtata
     1021 tgaaaacata tttactttct gtattacttt gtattgtagc tagtgttact gtgtattcgc
     1081 aagatataat tattttaaaa accggcgatg aaattcaatc gaaggtagaa gaaataagac
     1141 ccgatgtggt aacatatcgt aaatttgaga atttgcaggg acctttgtat accatagaaa
     1201 aaagtagaat ttttatgata aaatatgcta acggcagcaa agatgttttt agtgatataa
     1261 ctgttcagca acaacaacaa attcaacagt cgaatgctat tccaaaacct acacaacctg
     1321 caataacaga ggcaaataaa gaagagccgt tgcaatatat atttggaggt actattcgaa
     1381 gaaataatac aaatctaaca ccatttaaag ttaaagaaat aatgcgttct aatcctgaag
     1441 ctttttcatt atattcttcg ggccgaagtt taaaagttac cggcgatgta tttgaaggta
     1501 ttgggtatgg ttgtttcgtt tttatggtta ttaattcttt ggataataag gaaatacaag
     1561 cacgcagagc tgccggtttt ggaattgcta caatgagtac aagtttgata ttagaagcaa
     1621 taggaaaaag caatatgcgt aaagcagtaa atacctataa tgctgggtta aaaagcactt
     1681 cgtatttgtt tttaagtcca aattcgatgg gatattgttt gcgtttttag caccatatat
     1741 gttcaaacac aattttacaa gctataccta ttaaaactat tcctccaata gtttccattt
     1801 ttactgcata caatttttga aatcttccgc ctaaataaac accggcgaat gataatataa
     1861 atgtagtgat tcctattatg agaattgggc gagctaattc taatcgaagt aatccaaagt
     1921 ttactcctat aattaaagca tctatacttg tagcaaatgc taatcctaaa agtactatat
     1981 gtttttttgg gttaaaacag tgtttatttc ctttaatggc attttcataa atcatttttc
     2041 cgccaataaa taccagtaaa acaaatgcaa tccaatggtc aaatgattct atatattggg
     2101 caagttcttt gcctgccagc caaccgaata ttggcattac tccttgaaaa aaacccatat
     2161 ataccgcaat aggcattaca tctttaattt tgcatttttt gagaattaat cccgctgtga
     2221 ttgatacggc aaaactatcc attgccaacc ccaatgctat gagaaaaata tcgagaaaag
     2281 ccatatagta aaaatttgcc gcaaggtaca aaaacttata aaaaat
//
LOCUS       DAOTOW010000349         2324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35938_length_2324_cov_3.201410, whole genome shotgun sequence.
ACCESSION   DAOTOW010000349 DAOTOW010000000
VERSION     DAOTOW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35938_length_2324_cov_3.201410"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="P5243_06810"
     CDS             <1..2121
                     /locus_tag="P5243_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS19195.1"
                     /translation="KTFFIRFHLRMYYMFEHATSNFGTKMRENAGEPPVLFDEQLHNR
                     SMQQLNLYMHERGYYNAVVTSKIKTKGISDKKIAVKYKIKSGEVYTISNISYNIQDSV
                     LREIILSNTNKSILNNQSPFDISLLKQEQKRITTLINNSGYYSFNESNLYFTADTSKG
                     AKQVHLTLVVKDNGNTTDGTYKKSRIRNIYIYPDFEPSDLNRQTPYDTVIYSNHIYFL
                     YNNKNFLKPDVIQRANYIQLDSLYSISANDKTQRQLIQNKLFKLVTITYKETDLSDSS
                     KLENSLNCYIYITPHTRQLFTVEVEGTNTGGDWGAEINTSYMHKNIARGAENIYFKTK
                     ASGEYNRALKTDNEKNQLFNSYEYGGEIGFETPIFLSPIKPSKFDFNYRPKTNFKIAY
                     NYNKTVDYTKPTLQINYGYTWFGNNYLTHILKPVDISYIRIDNPSERLQLYIKNNEYI
                     KYSFEDYMIYSTNYTYIYYNRKPNSLQNYQYIRLFGETAGNALHAIYNAIDTSSQTSS
                     FKTFNVHFAQFAKIEADVRHYQILSKNSMVVYRLFAGMAVPYLNSEGLPSVKKYYAGG
                     ANSMRAWANRTLGPGSYIDSTNGFKFYMGDIKLEANIETRFHMFWYLDGAIFIDIGNI
                     WSYNDNTLQGAEFFASKFTKEIAIGTGYGFRFDFSFLILRLDLGLKVKEPYTITNTKS
                     SIIWGNRPLTNEDFNLNIAIGYPF"
BASE COUNT          902 a          373 c          314 g          735 t
ORIGIN      
        1 aaaacatttt ttattcggtt ccatttacgc atgtattata tgtttgaaca tgctacgagt
       61 aattttggta caaaaatgcg agaaaatgcc ggagagccac ctgttttatt tgacgaacaa
      121 cttcataatc gttctatgca acaattaaac ctgtatatgc acgaacgggg atattacaat
      181 gctgttgtta catcaaaaat taaaaccaaa ggcatttccg ataaaaaaat tgctgtaaaa
      241 tacaaaatta aatcaggaga agtatatact atatccaaca tttcatataa tattcaagat
      301 tcagtattac gagaaattat tctgtcaaat actaacaaaa gtattttgaa taatcaatca
      361 ccatttgata tttcattgtt gaaacaagaa caaaaaagaa ttacaactct gataaataat
      421 tccggatatt attcatttaa tgaatcaaat ttatatttta cagcagacac ttcaaaagga
      481 gcaaaacaag tacacctgac acttgttgta aaagataacg gcaatacgac tgacggcaca
      541 tacaaaaaat caagaattcg aaatatatat atttatcctg actttgaacc aagtgattta
      601 aatagacaaa ctccatatga tacagtaatt tattctaatc atatttactt cttatataat
      661 aacaaaaatt ttcttaaacc cgacgttata cagcgtgcaa attatattca acttgattca
      721 ttatacagca tttctgctaa tgataaaact caacgacaat taattcaaaa taaattattt
      781 aaattagtaa caattacata taaagaaaca gacttatctg atagttcaaa actagaaaac
      841 agtttgaatt gttatattta tataacacct catacacgcc aattatttac ggttgaagta
      901 gaaggcacaa ataccggtgg tgattgggga gcagaaataa acacatcata tatgcataaa
      961 aatattgctc gtggagccga aaatatttac ttcaaaacaa aagcttcggg tgaatacaat
     1021 agagcattaa aaactgataa cgaaaaaaat caattattta actcatatga atatggtggt
     1081 gaaattggat ttgaaactcc tatattttta tcacccataa aaccctctaa atttgacttt
     1141 aattatcgtc caaagactaa ttttaaaata gcctacaact ataataaaac ggtagattat
     1201 acaaaaccaa ctcttcaaat aaattacgga tatacctggt ttggcaataa ttatttaaca
     1261 catattttaa aaccggtaga tataagctat attcgtatag acaatccttc tgaacgcctg
     1321 caattatata ttaaaaacaa tgaatatata aagtacagtt ttgaagacta tatgatatat
     1381 tctacaaatt acacatatat atattataat cgcaaaccaa actctttgca aaattatcaa
     1441 tacattcgat tatttggtga aactgcagga aatgcattgc acgctattta caatgctata
     1501 gatacatcat cacaaacatc atcatttaaa acatttaatg ttcattttgc tcaatttgcc
     1561 aaaatagaag ccgatgttag acattatcaa attctttcca aaaattcaat ggttgtatat
     1621 agattgtttg caggcatggc agttccatat ttgaattccg aagggctacc tagtgtaaaa
     1681 aaatattatg ccggcggagc aaacagtatg cgagcatggg caaataggac attaggcccg
     1741 ggttcatata ttgattctac aaacggtttc aaattttata tgggcgatat aaaattagaa
     1801 gcaaatattg aaacacgatt tcatatgttt tggtatttag atggcgctat atttatagat
     1861 ataggtaata tatggtcata caatgacaat actcttcaag gggccgaatt ttttgcctca
     1921 aaatttacaa aagaaattgc cataggcaca ggatatggat tcagatttga tttttcattc
     1981 ctaatcctac gccttgactt aggattaaaa gtaaaagaac catatacaat tactaacaca
     2041 aaatcttcta taatatgggg caacagacca cttacaaatg aagattttaa cctaaatatt
     2101 gcaattggct atccatttta aatggttttt tcaaaaaaaa gttatttttt tgtaaaaaaa
     2161 ctactacaat gcttacaaaa gaaaacttta tactgttact attttcgttc tggtcatttt
     2221 ctctttttgc aacaacagaa ctttcatttt cattactcac aggcaatgca acacctcaaa
     2281 attatgactg ttttgccgaa gtacaatcaa tagaaaatgg tcaa
//
LOCUS       DAOTOW010000350         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35960_length_2323_cov_2.778660, whole genome shotgun sequence.
ACCESSION   DAOTOW010000350 DAOTOW010000000
VERSION     DAOTOW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35960_length_2323_cov_2.778660"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..829
                     /locus_tag="P5243_06815"
     CDS             152..829
                     /locus_tag="P5243_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRS19196.1"
                     /translation="METSIQYNIRKIKESIPNHVLLVAVSKTKPVSDIMQAYEIGIKD
                     FGENKVQELVAKYEQLPKDIRWHFIGHLQTNKVKYIAPFVSLIHGIDSLKLLQAINKE
                     AIKCNRIIPCLLQFHIAQEETKFGLSLQEAHEILQSHEFTTLHNIQINGVMGMASYVD
                     DSKQIQQEFKELYLIYKELKQKYFNNSEYFCQLSMGMSQDYTIAIEQGSTIIRIGSTI
                     FGERNLL"
     gene            complement(821..1333)
                     /locus_tag="P5243_06820"
     CDS             complement(821..1333)
                     /locus_tag="P5243_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19197.1"
                     /translation="MNSQIITKADIITLLHSNNIDNQLTAISEIRKNGDGDLLKQLLN
                     LLLSAQDFQVVREILRCISDLKDPNMLHILIESIQNPLFSSKKREILHALWQTNFDCS
                     AYCDYFISLIYTDSFEIGIEALTLLDVSCEYLSESIKSKYKNELMTYCNSTTDEQQLA
                     LINQAIDLLQ"
     gene            1499..>2323
                     /locus_tag="P5243_06825"
     CDS             1499..>2323
                     /locus_tag="P5243_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19198.1"
                     /translation="MREKLFKVVRYIALFGVITAFVSCNSESKNDSTDKEKELKAKVV
                     QDVKKVMISLPSPVETSILLKKAGASYNEALLNPTSNASKYNTDKQKALNLGVYGADL
                     SYASIFGQDQTIMQYMNISKKLAIGLDLLTAIDQSIIDRLEANHDNRDSVIRIISETF
                     MNSNSSLKDDNRPETAALILAGGWIEGLYLATSLVDKIEKKDLIERIVEQKMSLGELK
                     QLLETYKSNKDISDVLEAIQPIIKAFESIEIQKSDKVEVVTNLETKQTEMKQKQKIN"
BASE COUNT          929 a          272 c          348 g          774 t
ORIGIN      
        1 ggaacaactt atggcaatag ccgataatga ttgtataatg cctccaaaaa caacttggtt
       61 tgaacctaaa ttacgaagcg gattattact acattcttta gattaaatag taaagccttg
      121 tatgaacaag gcttttttta tttgtaaaat tatggaaacg tctattcaat ataatattcg
      181 taaaataaaa gaatctatcc caaatcatgt attattagtt gcagtgtcaa aaacaaaacc
      241 tgtgtcagat ataatgcagg cttatgaaat tggtataaaa gattttggtg aaaataaagt
      301 acaagaactt gttgctaagt atgaacagtt acccaaagat attcgttggc attttatagg
      361 acatttacaa acaaataaag taaaatatat tgcacctttt gtatcattaa tacatggaat
      421 agacagttta aaattattac aggcaataaa taaagaagca ataaaatgta atagaataat
      481 tccttgttta ttacagtttc atatagctca agaggaaaca aagtttggct tatcattaca
      541 agaggctcat gaaatattac aatcacatga atttaccact ttacataata ttcaaataaa
      601 tggtgtaatg ggaatggcat cgtatgttga tgattcaaaa caaatacagc aagaatttaa
      661 agaattgtat ttaatatata aagagctaaa gcaaaaatat tttaataata gtgaatattt
      721 ttgtcaatta tcaatgggaa tgtcgcaaga ctatacaatt gctatagaac aagggagtac
      781 aataattcga ataggttcta caatttttgg ggaacgaaat ttattgtaat aaatcaatag
      841 cttggtttat aagagccaat tgttgttcat cggttgtgga attacaatat gtcattaatt
      901 catttttata ttttgatttg atagattcag atagatattc acaagaaaca tcaagcagtg
      961 taagagcttc aatacctatt tcaaaagaat cggtgtaaat aagagatata aaataatcac
     1021 agtatgcact acaatcgaaa tttgtttgcc acaatgcatg taatatttct cgtttttttg
     1081 aagagaacag aggattttgt atgctttcta ttaaaatatg aagcatgttg gggtctttta
     1141 aatcagaaat acagcgtaat atttcacgta caacttgaaa atcttgagca cttaagagta
     1201 aattcaataa ttgtttcagt aaatcaccat caccgttttt tctaatttct gaaattgcag
     1261 ttaattgatt atcaatatta ttcgaatgca ataatgttat tatatctgct ttagtaataa
     1321 tctgtgaatt cataaaaaat attttttttc aaaaataaat aataaaaatt tgtcaatagc
     1381 agtgttattt aaacaattta tgttattttt gaattatgaa aaatattttt tgtaattaaa
     1441 aaaaatatat tatttttact caaacataaa tttgcatcaa taaataaaat taatgtctat
     1501 gcgagaaaaa ttgtttaaag ttgttcgtta tattgcttta tttggcgtaa taacggcttt
     1561 tgtgagttgt aattctgaat caaaaaatga ttcaactgac aaagaaaaag aattaaaagc
     1621 caaagttgta caagatgtaa aaaaagtaat gatttcatta ccatcacctg ttgaaacatc
     1681 aattttattg aaaaaagcag gagcaagtta taatgaagca ttattaaatc ctacgtcaaa
     1741 tgcttcaaag tataatacag ataaacaaaa agcattgaat ttaggagtat atggagccga
     1801 tttaagttat gcaagtatct ttggtcaaga tcaaactatt atgcagtata tgaatatttc
     1861 taaaaaatta gcaataggat tagatttatt aactgccatt gatcaatcaa ttatagatag
     1921 attagaagcc aatcatgata atagagattc tgtaatacga attatttctg aaacttttat
     1981 gaattcaaat tcatcgttaa aagatgacaa tcgtccggaa acagctgcat taatccttgc
     2041 cggtggttgg attgaaggtc tttatttagc tacaagtttg gttgataaaa ttgagaaaaa
     2101 agatttaatt gaaagaattg ttgaacaaaa aatgtcttta ggagaactaa aacaactatt
     2161 agaaacgtat aagtcaaata aagatatttc agatgtttta gaagcaattc aaccaattat
     2221 taaagctttt gaatcaattg aaattcaaaa atctgataaa gtggaagttg ttactaattt
     2281 agaaacaaaa caaactgaaa tgaaacaaaa acaaaaaata aat
//
LOCUS       DAOTOW010000351         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_35974_length_2322_cov_3.173798, whole genome shotgun sequence.
ACCESSION   DAOTOW010000351 DAOTOW010000000
VERSION     DAOTOW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35974_length_2322_cov_3.173798"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="P5243_06830"
     CDS             <1..1544
                     /locus_tag="P5243_06830"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRS19199.1"
                     /translation="KNKYYLFASKSGGYWEGTDLQSWKFIPCSTLPIEDYAPTAIVIG
                     DTLYFMASSHTKNAIYKTTQPETGKWHIACDSIEIPMWDPAFFLDDDNSLYIYWGCSN
                     QNPIYGAKMDYTNNFKLIAPPKVLVHAKPNEHGWEVPGDNNTLYNQNPWIEGAWVNKY
                     NGKYYLQYSGPGTEFKSYCDAVYVADSPLGPYTVAKHNPFSYKPSGFINGAGHGSTFA
                     DVYGNMWHIATATISQKHMFERRLVLYPVQYDAVGSMYTYTKFGDYPQYMPHKKITNS
                     ADIYTGWMLLSYNKKVTVSSWLPGFDPTNITDENIRTYWSARTGNRGEYAQIDLGELY
                     DVYALQINFAEHNTTKLGRNNTSGFTYTVEYSLDGTTWNILIDKSKNKEDISHDYIEL
                     PKKQACKYIRITNITVSDGTFALSGLRIFGKGNGPLPQPVQNFTAQRNETNKKQVTLS
                     WEKVPRATGYVISYGSQKDQLYSHYMVYADTTVVISSLSATQPYYYSIEAFNEFGISK
                     PTILQ"
     gene            complement(1536..>2322)
                     /gene="ispE"
                     /locus_tag="P5243_06835"
     CDS             complement(1536..>2322)
                     /gene="ispE"
                     /locus_tag="P5243_06835"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRS19200.1"
                     /translation="KINIGLRIVEKRNDGFHNIETVFYPIHGLYDCLEIHESQKLSFT
                     NTGLEIDAPIEKNLCVRAWQILNSYKSIPPVAIHLHKHIPFGAGLGGGSADAAFVLTA
                     LNSYYNLQLSVEILEKLALQLGSDCPFFIQSKPVFAEGRGELFSSCTINLANYFIVLA
                     NPNIHVSTTMAYAGVTPHKRPHTMLHDLSTNPHEWKGVIENDFETSVFKQFPAIKELK
                     HTMYNLGAIYAAMSGSGSTVYGIFENEVDVPHYIPVIWKGRLL"
BASE COUNT          804 a          415 c          398 g          705 t
ORIGIN      
        1 ataaaaacaa atattatttg tttgcatcaa aatcaggcgg ctactgggaa ggaactgatt
       61 tacaatcatg gaaatttatt ccgtgctcta cattacctat agaagattat gctcctacag
      121 ctatcgtaat aggagataca ttatatttta tggcttcgtc gcatactaaa aatgcgattt
      181 ataaaactac acaacccgaa accggtaaat ggcacattgc ctgtgacagt attgaaattc
      241 ctatgtggga tcctgcattt tttcttgatg acgataattc attgtatata tattggggat
      301 gttccaatca aaatccaata tatggtgcaa aaatggatta taccaacaat tttaaactta
      361 ttgccccccc caaagtatta gtccacgcca aaccaaatga acacggttgg gaagttcccg
      421 gtgataacaa tacattgtat aatcaaaatc catggataga aggagcatgg gtaaacaaat
      481 acaatggtaa gtactatttg caatattcag gtccgggtac cgaatttaag agctattgcg
      541 atgctgtata tgttgccgat tctcccttag ggccttatac tgttgctaaa cacaatccat
      601 tttcatataa accgagtgga tttataaatg gtgcaggaca tggcagtacc tttgccgatg
      661 tgtatggcaa tatgtggcat atagctactg ccacaatttc acaaaaacat atgtttgaac
      721 gtagattggt attgtacccg gttcaatatg atgctgtagg aagcatgtat acatatacaa
      781 aatttggtga ttatccacag tatatgccac ataaaaaaat tactaactcg gccgatatat
      841 ataccggttg gatgttactt tcgtacaata aaaaagttac tgtttcgtca tggttacccg
      901 gatttgaccc tacaaatata actgatgaaa atatccgtac ttattggtcg gcacgtaccg
      961 ggaacagagg agaatatgca caaatagatt taggagaatt atacgacgta tatgcattac
     1021 aaataaattt tgctgaacat aataccacaa aacttggtag aaataataca tcaggattca
     1081 cttatactgt agaatattcc cttgatggca caacatggaa cattttaatt gataaatcaa
     1141 aaaataaaga agatatatcg catgattata tagaattacc aaaaaaacaa gcatgcaaat
     1201 atattcgaat taccaatatt actgttagcg atggaacatt tgcattgagc ggactacgga
     1261 tttttggaaa aggaaacgga ccgttacctc aacctgtaca aaatttcaca gcacaacgaa
     1321 atgaaaccaa taaaaaacaa gttacactta gctgggaaaa agtacctcgc gcaacaggat
     1381 atgtaatttc gtatggctca caaaaagacc aattatattc acattatatg gtatatgcag
     1441 atactacggt tgttatttcc agtttatcgg caacacaacc atattattat agtatagagg
     1501 cgtttaatga atttggaatt tcaaaaccaa ccattttaca ataatctccc cttccaaatt
     1561 acgggtatat agtgcggaac atcaacttcg ttttcaaaaa taccgtaaac agttgacccg
     1621 ctaccactca tagctgcata tatagctcca agattataca ttgtatgttt taattccttt
     1681 attgcaggaa attgtttaaa aacagacgtt tcaaaatcat tttcaataac acctttccat
     1741 tcatgcggat tggttgacaa atcatgaagc attgtatgtg gtcgtttgtg aggagttact
     1801 ccagcatatg ccatagtggt actcacatga atatttggat ttgctaatac aataaaatag
     1861 ttcgccaaat taatagtaca agaactaaat agttcgccac gtccttctgc aaaaacgggt
     1921 tttgattgta taaaaaacgg gcaatcgctt cccaattgca atgcaagttt ttctaggatt
     1981 tcaaccgaaa gttgtaggtt ataatatgaa ttaagagcag ttaatacaaa tgcagcatcg
     2041 gcactcccgc ctcctaatcc tgccccaaaa ggaatatgtt tgtgtaaatg aatagcaaca
     2101 ggcggtattg acttatatga attgagaatt tgccaagctc taacacatag atttttttca
     2161 attggagcat ctatttccaa tcctgtattg gtaaaagaaa gtttttgtga ttcatgtatc
     2221 tctaaacagt catataatcc atgtatagga taaaaaactg tttcaatatt atgaaatccg
     2281 tcgtttcgtt tttcaacgat acgtaaacca atatttattt ta
//
LOCUS       DAOTOW010000352         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36147_length_2314_cov_2.849491, whole genome shotgun sequence.
ACCESSION   DAOTOW010000352 DAOTOW010000000
VERSION     DAOTOW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36147_length_2314_cov_2.849491"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1745
                     /locus_tag="P5243_06840"
     CDS             189..1745
                     /locus_tag="P5243_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRS19201.1"
                     /translation="MNKEIIIRAQDNDIDIALLEDSLLVEFITNKTNTAQFLVGDIYL
                     AKVKKIIPGLNAVFVDLGYEKDAFLHYHDLGPHFSSLNTFIQQIIQSKQIPTFSSFKQ
                     LPEIDKNGTIHDIIQPGQHILVQIAKEPISTKGPRLNSEISIAGRNIVLMPFSNKISI
                     SQKIESNEEKNRLKKILRSVVPQNYGVIVRTAAAGQDVAELEQEVNALVQKWEQTIKK
                     IKKYKNNTPHLVMSEMNKVSALLRDIFNPDFNNIIVNNKEIYEEIREYILSISPDKEK
                     IVKYYNAGPPIFEQFGVERQIKSSFGTTVPVKNGAYLIIEKTEALHVIDVNSGNRTKS
                     DKDQETNAYDVNAAAALEIARQIRLRDLGGIVIVDFIDMKSNENKQKLYEVLRDAMLR
                     DRAKHNILPLSKFGLMQITRQRVKPELKINTNEQCPVCNGSGHISPSINILYTFESDL
                     KHIYKVLKIKHVTLRLHPILAGYITKGLFSILLRWKFQIGFGIHVQTASNMNYLEYEY
                     IDKNNNIIIL"
     gene            1853..>2314
                     /locus_tag="P5243_06845"
     CDS             1853..>2314
                     /locus_tag="P5243_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19202.1"
                     /translation="MKVLIVEDDIVSRELLRVIVQKEGYELRTAEDGMAGFEVFSEYM
                     PDVVITDIRMPRLDGIELLKKIRTIERDTIVIIVTAHGNENIALEALHMGANNYLKKP
                     INLEDLKIILSRYHSLYKTKTVRKDITALITSQHLLLSIESDLDLIPSVAEY"
BASE COUNT          901 a          348 c          331 g          734 t
ORIGIN      
        1 aacaacttaa aatattaatc ctttgttgct taggcaataa aggattaata attaacacga
       61 aattctgatt ttttatatcc tttatttagt ataaatcaga aaccaccaaa aaacaacata
      121 aactcaatat gattgtaaat aaatgttaca attcatactt attgttaaga aacatttaat
      181 tattcgaagt gaataaagaa ataataatac gagcacaaga taacgatata gacatagcac
      241 tgctcgaaga ttcgctttta gtagaattta ttaccaataa aacaaataca gctcaatttt
      301 tagttggtga tatttattta gcaaaagtta aaaaaattat tcctggactc aatgctgtat
      361 ttgtagattt aggttatgaa aaagatgcat ttttacatta ccacgattta ggtcctcact
      421 ttagttcact taacacattt attcaacaaa taattcaatc aaaacaaatt ccaacgttta
      481 gttcgtttaa acaacttccc gaaattgata aaaacggtac tattcatgat attattcaac
      541 ctggacaaca tatactggta caaatagcaa aagaacctat ttcaactaaa ggacctcgtt
      601 taaattctga aatttctatt gcaggtagaa atattgttct tatgccattt tccaataaaa
      661 tatctatatc gcaaaaaata gaatcaaacg aggaaaaaaa tagactaaaa aaaatactgc
      721 gttcggttgt tcctcaaaat tatggtgtta tagttcgaac tgctgccgca ggtcaagatg
      781 tagccgaact tgaacaagaa gttaatgctc ttgttcaaaa atgggaacaa accataaaaa
      841 aaattaaaaa atataaaaac aacactcctc atttagtaat gagcgaaatg aacaaagttt
      901 ctgctctgtt acgcgatatt ttcaatcctg attttaataa tataattgta aacaacaaag
      961 aaatatacga agaaatacgt gagtatattc tttctatttc accagacaaa gaaaagattg
     1021 taaaatatta taatgccggc cctcctattt ttgaacaatt tggtgtagaa cggcaaatta
     1081 aaagttcttt cggaactaca gttcctgtta aaaacggagc atatctaatt attgaaaaaa
     1141 ctgaagcatt acacgtaata gatgtaaata gtggaaatag aaccaaatca gataaagacc
     1201 aagaaactaa tgcctatgat gtaaatgcag cggcagcatt agaaattgcc cgtcaaatac
     1261 gacttcgtga tttaggtgga atagtaatag ttgattttat tgacatgaaa tcaaacgaaa
     1321 ataaacaaaa attatacgag gtactacgtg atgctatgtt gcgtgataga gcaaaacata
     1381 atatactccc attaagtaaa tttggtttaa tgcaaattac tcggcaacga gtaaaacctg
     1441 aacttaaaat aaacacgaat gaacagtgtc cggtttgtaa cggcagcgga cacatttctc
     1501 cttctattaa cattttatat acttttgaaa gtgacttaaa acatatttat aaagttctga
     1561 aaattaaaca tgtaactcta cgcttacatc ctattcttgc aggatatatt acaaaaggat
     1621 tattttctat tttattgcgt tggaaatttc aaattggatt tggcattcat gttcaaacag
     1681 caagcaacat gaattattta gaatatgaat atattgataa aaacaataat ataatcatat
     1741 tataaatcct ataatatttt tctttttctt tgaaaatcgg ctttttttta tatacctttg
     1801 ttaatagata gtatatttac gtaatctatc ttacacaatg taacaaacaa caatgaaagt
     1861 acttatagtc gaagatgata ttgtttcacg tgaattactc agagtaattg tacaaaaaga
     1921 agggtatgaa cttcgtactg cagaagatgg tatggctggt tttgaagtat tttctgaata
     1981 tatgcccgat gttgttatta cagatattcg aatgcctcgt cttgacggaa tagaattatt
     2041 aaaaaaaatt agaactatag aacgagatac tattgttata atagttactg ctcatggtaa
     2101 tgaaaacata gctcttgaag cccttcatat gggagcaaat aattatttaa aaaaacctat
     2161 caatttagaa gaccttaaaa ttatactttc acgctaccat agtttatata aaacaaaaac
     2221 cgtacgtaaa gacattaccg cattaataac atcacaacat ctgctattaa gtattgaatc
     2281 cgacttagat ttaataccgt ctgtagcaga atat
//
LOCUS       DAOTOW010000353         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36158_length_2313_cov_4.853853, whole genome shotgun sequence.
ACCESSION   DAOTOW010000353 DAOTOW010000000
VERSION     DAOTOW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36158_length_2313_cov_4.853853"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..742
                     /locus_tag="P5243_06850"
     CDS             158..742
                     /locus_tag="P5243_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19203.1"
                     /translation="MDFRILLFLVFLYFSKITYAQDDTVRHYPITIGVSFGDPDFVSL
                     QCQYLNKSAPFRVGISGTYIPHRNVVYTLGSCNIDYYLTPYKTWHAGMQYAYREIFTK
                     NDGIDKDNGYTYIYNDIYKTNIYGLYGGFDIRWNRLLITPQVGAQLQRTIFNSYEKII
                     YTNNSDIREISIPYENLNQNLILPLIRIHVGFCI"
     gene            complement(800..1618)
                     /locus_tag="P5243_06855"
     CDS             complement(800..1618)
                     /locus_tag="P5243_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HRS19204.1"
                     /translation="MKGKYSNLIIIGIIGVIVLILFGGSMFIKLEATERGVMFRQFTT
                     GLDTVQVYQPGFHIIAPWNDMYVFNVAEQKIEEPMDVLDKNGLSINIDVTVRFYPVYT
                     RVPFLYRAFKLDYISVLVVPEVRSAVRQITGRYSAEEIYSTKRSEVEQKIIDEAGKKL
                     RDNNIEMKALLIRSINLPPDIRQAIESKLKQEQEALAYKFRLDKENSEAERKRIAAEG
                     EARANKIINNSLTEELLKMRGIEATLKLGESPNSKVVIVGGGKSGLPIILNTDK"
     gene            complement(1731..>2313)
                     /locus_tag="P5243_06860"
     CDS             complement(1731..>2313)
                     /locus_tag="P5243_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19205.1"
                     /translation="TGTQSTFYFLLTPRNNKALASERGKQVISNIIHRNHSFGIHLNE
                     WTEAEFPLIQKTITQFETIAESKITKSRQHFLKIQVPDTLRVLEQAGIYQDSSLYYRN
                     NPGFRTGMCIEHSMFDCINRKTMSIKELPLILMDVSLQKYSQIEDAQTLIYTLIQTTK
                     KYSGNFVCLWHNSSFDYHTWKNGDELYSYLIEI"
BASE COUNT          726 a          356 c          357 g          874 t
ORIGIN      
        1 taaagtttta tctttgcaat taagttatca taaacaacct tgtgaaactt gacatgtatg
       61 ggtagatata tgcgaaattc ttttgtatga ttttcgtcat ttttgtactt ttttctatac
      121 atttttttat tcaactaatt ttttaaaaca tataataatg gattttagaa ttttattgtt
      181 cttagttttt ctctattttt ccaaaattac gtatgctcaa gacgatactg taaggcacta
      241 tccaataact ataggtgtaa gttttggcga tcccgacttt gtgtctcttc aatgtcaata
      301 tttgaacaaa tcggctcctt ttcgtgtagg tatttcagga acctatattc ctcatagaaa
      361 tgtagtctac acgttaggtt catgtaatat tgattattat ttaacaccgt ataaaacatg
      421 gcatgcggga atgcaatatg catatagaga aatatttaca aagaatgatg gaatagataa
      481 agataatgga tatacatata tttataatga tatatataaa acaaatatat atggccttta
      541 cggaggtttt gatatacgat ggaatcgttt gttaatcact ccacaagttg gagcacaact
      601 gcaaagaacg atttttaatt cgtatgaaaa aattatctat acaaacaatt ctgatataag
      661 agaaataagc attccatatg aaaatttaaa tcaaaatctc atattacctc ttattcgaat
      721 acatgtaggt ttttgtatat aaacaacaaa gcccgaagaa ttttccgggc tttgtgtatc
      781 ttatatgttg taattattct tatttatcgg tgttcaatat gataggtaat ccgcttttgc
      841 ctccgcctac tattaccact ttagaatttg gcgattcacc taatttaagt gttgcttcta
      901 taccacgcat tttcaataat tcttctgtta aactattatt gatgatttta ttagcacgag
      961 cttcaccttc ggcagctatt cgtttacgtt ctgcttcact attttctttg tctaaacgaa
     1021 atttgtatgc aagtgcttct tgttcttgtt tcaatttgct ttcgatagct tgacgaatat
     1081 cgggaggtaa attaatagaa cgaataagta aggctttcat ttcaatgttg ttgtcgcgaa
     1141 gttttttacc tgcttcgtca attatttttt gttctacttc gcttcgtttg gtcgaataaa
     1201 tttcttctgc cgaatatcta cctgtaattt gtcgaactgc cgaacgaact tcgggaacaa
     1261 caagtacact aatgtaatcg agtttaaaag ctctatataa aaaaggcaca cgagtatata
     1321 ccggataaaa acgaacagtt acatcaatat taatagaaag accatttttg tctaacacgt
     1381 ccattggttc ttctattttt tgttctgcta cgttaaatac atacatatca ttccatgggg
     1441 caataatatg aaatccgggt tgatatactt gaacagtgtc taaacctgtt gtaaactgac
     1501 ggaacataac gcctcgttct gtcgcttcaa gttttataaa catactacct ccaaataata
     1561 tgagaacaat aacgcctatt atacctataa ttatgagatt tgaatattta cctttcattg
     1621 ttttttaaat ttaagtttca agtaattttt ttgtaaacat acacaaatta ttatattaat
     1681 tcgaaaaaaa acgtacaaac cgtatcatag ataaaaatga aattatgcat ttaaatttca
     1741 attaaatagg aatacaattc atctccgttt ttccatgtgt gatagtcgaa actactgttg
     1801 tgccataaac aaacaaagtt tcccgaatat ttcttagtag tttgtattaa ggtgtaaatt
     1861 agtgtttgtg catcttctat ttgtgagtat ttttgtaagc ttacatccat taagattaga
     1921 ggtaattctt tgattgacat agttttacgg ttaatacaat caaacatgga gtgttcaata
     1981 cacataccgg ttctaaatcc gggattgtta cggtaatata atgaactatc ttgatatatt
     2041 cctgcttgtt ctaaaactcg caaagtatcg ggtacttgta tttttaaaaa atgttgtcta
     2101 ctttttgtaa ttttagattc cgcaatagtt tcaaattgtg ttattgtttt ttgaatgagt
     2161 ggaaattctg cttctgtcca ttcatttaaa tgtattccaa atgaatgatt ccgatgtatt
     2221 atattgctta ttacttgttt gccgcgttct gaagcaagtg ctttattatt gcgtggagta
     2281 agtaagaaat aaaacgttga ttgggttcct gtt
//
LOCUS       DAOTOW010000354         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36193_length_2312_cov_3.363314, whole genome shotgun sequence.
ACCESSION   DAOTOW010000354 DAOTOW010000000
VERSION     DAOTOW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36193_length_2312_cov_3.363314"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="P5243_06865"
     CDS             complement(<1..188)
                     /locus_tag="P5243_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19206.1"
                     /translation="MTNHVRLYILIACVAIISVSCKQEKKQEKQKTKPTVQVTVMCAG
                     NQTISNSIHVSGTVQANE"
     gene            complement(282..1211)
                     /locus_tag="P5243_06870"
     CDS             complement(282..1211)
                     /locus_tag="P5243_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRS19207.1"
                     /translation="MITLADIQQHHEFDNFELLAQQIVEGFITGLHRSPFHGFSVEFA
                     EHRLYNSGESTKHIDWKLFAKTDKLFVKRYEEETNLRCRIVIDTSSSMLFPFGNSSIY
                     NKLGFSLYSAAALIYLLRKQRDAVGLSLFNNGVYVHTDAKLSATHAHMLYKQLYTTLQ
                     ESYSSESFKQNTSLAQSLHQIAETTHTRSLIIIFSDFFENTNYEDVFAALQHCKYNKH
                     EVLVFHVTEPNHEEQFAFENRPLKITDMETGEIIKIQPETVRNTFKANVESIVQSIAH
                     MCTQYQIDYIKADINQGFSHVLLNYLIKRNKLM"
     gene            complement(1241..1558)
                     /gene="trxA"
                     /locus_tag="P5243_06875"
     CDS             complement(1241..1558)
                     /gene="trxA"
                     /locus_tag="P5243_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRS19208.1"
                     /translation="MAVEITEANYNEVVAQSDKLVMIDFWAEWCGPCRMVAPIVAELA
                     TEYEGKAVIGKCDVDENSEIAVKFSIRNIPTLLFVKNGEVVDKLVGAVPKAKIVEKLD
                     AYL"
     gene            1755..>2312
                     /locus_tag="P5243_06880"
     CDS             1755..>2312
                     /locus_tag="P5243_06880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRS19209.1"
                     /translation="MRLLSILFLIFLQVICYSQQKVSLLFMGDVMGHDGQLQAAYNPE
                     KMIYEFDTCFSYISPIISLYDFAIANLEVTLNCTPYKGYPSFSSPASLASALVAHGVD
                     CFVTANNHSADRGKTGVINTIKALDSIGVMHTGTFINVKERKKTYPLIFEKNGIRIAL
                     LNCTYGLNGLPVPEGTVINLIDTLQI"
BASE COUNT          721 a          344 c          396 g          851 t
ORIGIN      
        1 atttcgtttg cttgtactgt tccgcttacg tgtatgctgt ttgaaatagt ttgattaccg
       61 gcacacataa ccgttacctg taccgtaggt tttgtttttt gtttttcttg ttttttttct
      121 tgtttacaag atacacttat gatagcgaca caagctatta atatatataa gcgaacatga
      181 tttgtcatta gatattttta tttcgtaaaa atacatgtat ttggtttgta aacaaataaa
      241 tatagacaaa aacctttgtt gtatagatga aaggtcaata tttacataag tttattgcgt
      301 tttattaaat aattaagcag tacgtgtgaa aatccttggt taatatcagc ttttatgtaa
      361 tctatttgat attgtgtgca catgtgagct attgattgta ctatagattc tacatttgct
      421 ttgaacgtat ttcgtacagt ttcaggttga attttaatta tttcgcctgt ttccatatcg
      481 gtaattttaa gtgggcgatt ttcaaaagca aattgttctt cgtgatttgg ttcggtaaca
      541 tgaaatacta acacttcatg tttattgtat ttgcaatgtt gcaatgctgc aaaaacatct
      601 tcataattag tattctcaaa aaaatcactg aagataatta taagtgatcg cgtatgtgtg
      661 gtttcggcta tttgatgcag cgactgagct aatgatgtat tttgtttgaa agattcagat
      721 gagtatgact cttgaagtgt agtgtatagt tgtttatata gcatgtgcgc atgtgttgcc
      781 gataattttg catcggtatg cacatacaca ccattgttga ataagcttaa acctacagcg
      841 tcgcgttgtt tgcgaagcag ataaataagt gctgctgccg aatacaatga aaatcctaat
      901 ttattatata tagaagaatt gccaaacgga aaaagcatag atgaagatgt gtctattact
      961 atacggcatc ttaaattagt ttcttcttcg tatcgtttta caaataattt atcggttttt
     1021 gcaaatagct tccaatctat atgtttggta gattctcccg aattatagag tctatgttct
     1081 gcaaattcta ccgaaaaacc atgaaaagga cttctgtgca atcctgtaat aaacccttca
     1141 acaatttgct gagccagtaa ttcaaaattg tcaaattcat gatgttgttg tatatcggcc
     1201 agagtaatca tgtatttgta tgtttagcta caaatatata ttacaaataa gcatctaatt
     1261 tttctacaat ttttgcttta ggaactgcac ctacaagttt atctacaact tcaccgtttt
     1321 taacaaacag aagtgtaggt atgttgcgga tagaaaattt cacggcaatt tccgaatttt
     1381 cgtcaacatc gcatttgcct attacggctt taccttcata ttcggttgca agttctgcta
     1441 ctataggagc aaccatgcgg catgggccac accattctgc ccaaaaatct atcattacaa
     1501 gtttgtctga ttgtgctact acttcgttgt agtttgcttc ggtaatttct actgccataa
     1561 ttatacgttt ttatattttt tgacacattg tttctaaatt ctttacaaag taatacaatt
     1621 tagccgaaga atgctataaa aagttgaaaa attgtagata agttagcttt tgtattcata
     1681 gattgatttg ctatgttttt tgctaaaaat gtaatatact tgtaaaaaaa atgttgagta
     1741 taaaaatgaa cataatgaga ttattgagta tattattctt aatttttcta caagtcattt
     1801 gttattcaca acaaaaggtt agtcttttgt ttatgggcga tgtaatggga catgacggac
     1861 aattgcaggc tgcgtataat cctgaaaaaa tgatatatga atttgataca tgtttttcat
     1921 acatatcacc aattatttca ttatatgatt ttgcaatagc taatttagag gttacgttaa
     1981 attgcactcc ctataaagga tatccatctt ttagttcgcc tgcatcgtta gcgtcggctc
     2041 ttgttgcaca tggtgtagat tgttttgtaa cagccaataa tcatagtgcc gatagaggaa
     2101 aaacaggtgt tataaatact attaaagcat tagattctat tggggtaatg catacaggaa
     2161 cgtttataaa tgtaaaagaa agaaaaaaaa cgtatccctt aatttttgaa aaaaatggta
     2221 ttcgaatagc tttactcaat tgtacatatg gacttaacgg attaccggtt cccgaaggta
     2281 ctgtaattaa tttgattgat acattgcaaa ta
//
LOCUS       DAOTOW010000355         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36217_length_2311_cov_3.464096, whole genome shotgun sequence.
ACCESSION   DAOTOW010000355 DAOTOW010000000
VERSION     DAOTOW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36217_length_2311_cov_3.464096"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="P5243_06885"
     CDS             <1..666
                     /locus_tag="P5243_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRS19210.1"
                     /translation="LSVAIMQRINIFNKTILAYVGSVSVIIAGLLFYFKGLSKEQVEI
                     VSNVAGNGIIFSVIIAFIVAGVIKKINVFDAFIEGGKEGFSVAIKIIPYLIAILVGIG
                     VFRNVGGLQVIIDGLSWFFNTLNINTDFVPALPTAIMKPLSGTGARGMMLEAMNSYGA
                     DSFVGRLACIMQGATDTTFYIIAVYFGSVGIKKTRHAIGCGLIADITGIVAAIIIAYM
                     LFY"
     gene            679..1221
                     /locus_tag="P5243_06890"
     CDS             679..1221
                     /locus_tag="P5243_06890"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HRS19211.1"
                     /translation="MIFLEKIYHYFQLKKIPLIGLYVLSNDVYFSTIFLKKISIDTPH
                     YFKTYKHCDECLLELQTNSTIKHILPFIVIDNSIFLEQTPVTNKNEFIEAVFANAPNI
                     HVVQLDNAELQNFVKDYNNTIISKNENSYKRVEQIIFTVCNDFTIIQQKKYNKIIFIV
                     LSIIIILIISFLSFYIVQKL"
     gene            1337..2239
                     /locus_tag="P5243_06895"
     CDS             1337..2239
                     /locus_tag="P5243_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19212.1"
                     /translation="MSDNQVLQDDLEKAKQEALAKLQEIEKLQQQTSQKTETQSTIEN
                     KEVTAQPEIKKPVTQTVEKETKTSKVEKKIDNKAKEEEQLKNYNVSITNRTKGKVPAE
                     PGNKGVKHTNCEVFIFLVDDNELQLKVLQEQFKNTRSFKKTKAFTNGKDLLDYIKTSK
                     FPKNSIFLVVMDYFLEDSDDEEAQNGIAVLNELKSYDPNIEVIMLSSHADVDIAASAT
                     YFGAVTFIQKGKDAFNKIINNIVWAIHEQEKLRKKADAKKLIKIGIIGFLSVIAILII
                     ADIVTDGALGIIPVMNQPPVPSTK"
BASE COUNT          920 a          329 c          342 g          720 t
ORIGIN      
        1 ttatcggttg ccataatgca acgaatcaat attttcaata aaacaatact tgcatatgta
       61 ggttctgtaa gtgtaattat agccgggtta ttattctatt tcaaaggatt atcaaaagaa
      121 caagtagaaa tagtatcaaa tgttgcagga aatggaatta tattttcggt aatcatagca
      181 tttatagtag caggtgtaat aaaaaaaata aatgtatttg acgcatttat tgaaggcggt
      241 aaagaaggat ttagcgtagc aattaaaata attccgtatt taatagctat tttagtaggg
      301 ataggagtat ttagaaatgt tggtggatta caagtaataa tagacggact gtcatggttt
      361 tttaatacac tcaatataaa taccgacttt gtacctgccc tgcctacagc tataatgaaa
      421 ccactcagtg gaaccggtgc ccgcggaatg atgcttgagg caatgaattc atatggagcc
      481 gatagttttg taggacgatt ggcctgcata atgcaaggag caacagacac caccttttat
      541 ataatagcag tatattttgg ctcagttggt attaaaaaaa cccgtcatgc aattggttgc
      601 ggtcttattg cagatattac aggtatagtt gcagctatta tcattgcata tatgttattt
      661 tattaaaaaa acggtactat gatattctta gaaaaaatat atcattattt tcaactcaaa
      721 aaaataccat taatagggtt gtatgtatta tcaaacgatg tatatttttc aacaattttc
      781 ttaaaaaaaa tatcaataga cactccccac tattttaaaa catataaaca ctgcgatgaa
      841 tgcttattgg aactacaaac caattcaacc attaaacaca ttttaccatt tattgtaata
      901 gacaattcca tttttttaga acaaacacct gtaaccaata aaaatgaatt tattgaagct
      961 gtttttgcaa atgctccaaa tattcatgtg gttcaattag acaatgctga attacaaaat
     1021 tttgtaaaag attataacaa cactattatt tcaaaaaatg aaaattcata taaacgagta
     1081 gaacaaataa tatttaccgt ttgtaatgat tttacaataa ttcaacaaaa aaaatataat
     1141 aaaataattt ttattgttct ttctattata ataattttaa ttatatcatt tttaagtttt
     1201 tacatagtac aaaaactgta aaatcctgca tattgttaat tgttgtttaa taaaaaacta
     1261 tggaatattt tatttttaat taaaaaaaca ctatttttat gttctgaaac tatgttcaat
     1321 taaaaaaata aacctcatga gtgataatca agtgttacaa gacgacttgg aaaaagctaa
     1381 acaagaagcg ttagcaaaac ttcaagagat tgagaaatta caacaacaaa cttctcaaaa
     1441 aacagaaaca caatcaacta tagaaaataa agaagtaaca gcacaacccg aaattaaaaa
     1501 accggttact caaacagttg aaaaagaaac taaaacttca aaagttgaga aaaaaataga
     1561 taataaagct aaagaagaag aacagcttaa aaactataat gtttctataa ctaatagaac
     1621 gaaaggaaaa gttccggcag aaccgggcaa taagggagta aaacacacaa attgtgaagt
     1681 ttttatattt ttagttgatg acaatgaatt gcaacttaaa gtattgcaag aacaattcaa
     1741 aaacaccaga agtttcaaaa aaaccaaagc atttaccaat ggtaaagatt tattggatta
     1801 cattaaaact tctaaattcc ctaaaaattc tatcttttta gtagtaatgg actacttctt
     1861 agaagattct gatgacgaag aagcacaaaa tggtattgcg gttcttaacg aactcaaatc
     1921 atacgaccct aatattgagg taatcatgct ttcgagccat gccgatgtcg atattgctgc
     1981 ttctgcaacg tattttggtg ctgttacatt tattcaaaaa ggtaaagatg catttaataa
     2041 aattataaat aatatagtat gggctataca cgaacaagaa aaacttcgta aaaaagctga
     2101 tgcaaaaaaa cttattaaaa taggtattat aggattttta tcggtaatag ctatattaat
     2161 tattgcagac attgtaacag acggagcgtt aggtattatt cctgtaatga atcaacctcc
     2221 ggttccttca acaaaataat cagttttttt gtcagtagac caccctaaac aatgacaata
     2281 tgacataaaa aaactaacgg catatcattt g
//
LOCUS       DAOTOW010000356         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36220_length_2311_cov_3.132979, whole genome shotgun sequence.
ACCESSION   DAOTOW010000356 DAOTOW010000000
VERSION     DAOTOW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36220_length_2311_cov_3.132979"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..268)
                     /locus_tag="P5243_06900"
     CDS             complement(23..268)
                     /locus_tag="P5243_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRS19213.1"
                     /translation="MPQRINWIDYAKVIGIYLVVLGHLPIPEQGSVYIYSFHMPLFFF
                     ISGFLSKTNNITFRQFILKKIRTLIIPYHYCPIKLQN"
     gene            complement(271..711)
                     /locus_tag="P5243_06905"
     CDS             complement(271..711)
                     /locus_tag="P5243_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19214.1"
                     /translation="MNTGLQYQIFPDKKLIVEYYSGEIYKNTIQYFKKEISKNVAYNM
                     HYNIIHDFRDATLVANIEEVRSFIDYIKQNTNTYAQKKIAFITNNPNQVSITYLFSTL
                     KKETLLIAETFSTVEGAIMWVGLQPTETDFIQSVLEELKQLSQL"
     gene            885..2297
                     /locus_tag="P5243_06910"
     CDS             885..2297
                     /locus_tag="P5243_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS19215.1"
                     /translation="MKAYLQDTVLQIVSDIVTQQNQQAYVIGGYVRDIFLKRPNKDID
                     IVTLGDGIELAEQTANRISPNITVSVFKNFGTAMFKYRDCEYEFVGARRESYTHNSRK
                     PIVESGSLRDDQNRRDFTINALAISLHSQTFGELLDPFNGVEDINKKIIRTPLNPDIT
                     FSDDPLRMMRAVRFATQLGFRIESETYNALQRNKSRLAIVSKERIIDEFNKIMSSAKP
                     SIGIVLLESSGLLQEFFPELQALKGVEYVGGHTHKDNFFHTLEVLDAVCAKSNNMWLR
                     WAALLHDIAKPRTKKYIEGTGWTFHGHEFMGAKMIPEIFKKLKLPLHEPMKYVQKLVS
                     LHLRPIALVDSHITDSAIRRLLFDAGNDIDDLMLLCHADITSKNEQKVKTLHANFEHV
                     VRKLKQVEEKDAIRNWQPPISGELIIETFGIKPSREVGIIKDAIREAILDGEIENTYD
                     SAYSYMLEQGIKLGLQVTKK"
BASE COUNT          815 a          309 c          388 g          799 t
ORIGIN      
        1 aaataaattt tgttttatta aattaatttt gtagttttat cggacaataa tgatatggaa
       61 ttataagggt gcgaattttt tttaaaataa attgtcgaaa cgtaatatta ttagtttttg
      121 ataaaaagcc tgaaataaag aaaaaaagtg gcatatgaaa cgaatatata tatacactac
      181 cttgctccgg tataggtaaa tgacctaata ctactaaata tatacctatt acttttgcgt
      241 aatctatcca atttattctt tgcggcatgg ttataattgt gataattgtt ttaattcttc
      301 aagaactgat tgtataaaat ctgtttcggt aggttgtagt cctacccaca taatggcacc
      361 ttcaactgta ctaaaagttt cggctattaa gagagtttct ttttttaatg tgctaaataa
      421 atatgttata gaaacttgat tgggattgtt ggtaataaat gcaatttttt tttgtgcata
      481 ggtgttggta ttttgtttta tataatcaat aaatgaacga acctcttcaa tattagcaac
      541 taatgtggca tctctaaaat catgtattat attgtaatgc atattatatg ctacattttt
      601 gcttatttct tttttgaaat actgaatagt atttttgtaa atttctccac tataatattc
      661 tacaataagt tttttgtcag gaaaaatttg atattgtaag ccggtattca ttattaaact
      721 attactctct catttatata tatcaaagat aagtaaaatt acttaataaa caatttttct
      781 atattctaat ttgtaagaca actctaagtt agaaagcttt tttttgagaa caactttcat
      841 taatagtaaa tgttcatata tctttgcgga agtaatttaa agtaatgaaa gcatatttac
      901 aagataccgt tttacaaatt gtttcagata tagttactca acaaaatcaa caggcatatg
      961 ttattggagg ctatgtgcgt gacatttttt tgaaacgtcc aaataaagat attgatatag
     1021 ttacacttgg cgatggaatt gaattggcag agcaaactgc gaacaggata tcaccaaaca
     1081 taacagtatc tgtattcaag aattttggta ccgcaatgtt taaatatcgt gattgtgaat
     1141 atgaatttgt tggtgcacga agggaatcat acacacataa ttctcgaaaa ccgattgtag
     1201 aatcaggttc gttacgcgat gaccaaaaca gacgagactt tacaattaat gctttggcaa
     1261 tttctcttca ttctcaaaca tttggtgaat tgctcgaccc ttttaatgga gtagaagata
     1321 ttaataaaaa aattattcga acaccattaa atcctgatat aacgttttct gatgatcctc
     1381 tgcgaatgat gcgtgcagta aggtttgcaa ctcaattggg atttagaatt gaatcggaaa
     1441 catacaatgc actgcaaaga aataaatcga gacttgctat agtttcaaaa gaacgtatta
     1501 tagatgagtt taataaaatt atgtcttcag caaaaccttc cattggtatt gttttgttgg
     1561 aaagtagcgg tttgttacaa gaattttttc ctgaattaca agcactcaaa ggagtagaat
     1621 atgtaggagg acatacacat aaagataatt tttttcatac tttagaggtg cttgatgctg
     1681 tatgtgccaa aagcaacaat atgtggctta ggtgggcagc attattgcat gatatagcaa
     1741 agcctagaac aaaaaaatat attgaaggta ccggttggac ttttcatgga catgagttta
     1801 tgggggctaa aatgatacca gaaatattta agaaattaaa actaccattg catgaaccta
     1861 tgaaatatgt tcaaaaatta gtttcattac atctacgtcc aattgctttg gttgattctc
     1921 atattactga ttcggctatt cgcagattgt tgtttgatgc cggtaatgat attgatgatt
     1981 taatgctgct ttgtcatgcc gatattacat caaaaaatga acaaaaagta aaaacattgc
     2041 atgctaattt tgaacatgta gttagaaaac ttaaacaagt tgaggaaaaa gatgcgatcc
     2101 gtaattggca accgcctatt tcaggagaat taataataga aacatttggt attaaacctt
     2161 ctcgtgaagt aggaataata aaagatgcta tacgagaagc aatacttgat ggtgaaattg
     2221 aaaacacata tgattctgca tattcatata tgcttgaaca aggaataaaa ctaggcttac
     2281 aagttacaaa aaaataggtt cttttatgaa a
//
LOCUS       DAOTOW010000357         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36253_length_2309_cov_3.612245, whole genome shotgun sequence.
ACCESSION   DAOTOW010000357 DAOTOW010000000
VERSION     DAOTOW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36253_length_2309_cov_3.612245"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="P5243_06915"
     CDS             <1..744
                     /locus_tag="P5243_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRS19216.1"
                     /translation="WPDNMICYMPEEKILFSNDSFGQHYATSGRFDDEVSHDEALYEA
                     KKYFANIVLPYSAQVIKELEAASKFEIELICTSHGVIWKKYIAEIIQEYNKWARNVTK
                     KKVVIAYDTMWKSTEKMAYAIYEAFEQEGYEIALRNLQVNHESDVMTDVIDAEYICVG
                     SPTLNSEMMTNVVGFLTYMRGLAPKGGRKAVAFGSYGWNGKSIPGVEQFLKDCNYDVK
                     AVFTHQYRPTKEDLQQITTKTLEIIKQNN"
     gene            750..1124
                     /locus_tag="P5243_06920"
     CDS             750..1124
                     /locus_tag="P5243_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HRS19217.1"
                     /translation="MTKRSEIYQCGLCGNIVEVLHQAPGKLVCCGQSMNLLEENSKDA
                     ATEKHVPVITKIEGGYKVTVGEVLHPMQPEHYIEWIELIAGNEIFRKYLQPGETPEAI
                     FYTQATDVYAREFCNLHGNWKS"
     gene            1263..2159
                     /gene="gnd"
                     /locus_tag="P5243_06925"
     CDS             1263..2159
                     /gene="gnd"
                     /locus_tag="P5243_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRS19218.1"
                     /translation="MKIGFIGLGKMGGNMVQRLLLDKHEVVVFDFNTSIVKDLEKKGA
                     IAATDIEDMIQKLPDTKVVWLMVPAGKPVDDTITKLLPLLQKGDILIDGGNSFWKDSQ
                     ARAQRTAFKSVEFVDCGTSGGIWGLQNGYCLMYGGNKQTCDSLEPIFKSLATDNGYLY
                     CGESGAGHFVKMVHNGIEYGMMQAFAEGFELMEKSPFTPDLEKVAELWRHGSVVRSWL
                     LDLIKNALDEDPKLETITDYVDDNGEARWMIQSAMDFEVPVHVTAASLFTRFESRQNE
                     SFAMKMLAAMRNQFGGHAVKTK"
     gene            2161..>2309
                     /locus_tag="P5243_06930"
     CDS             2161..>2309
                     /locus_tag="P5243_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19219.1"
                     /translation="MNSKDKHILVIFGGSGDLTQRKLIPALYDLHRKKMLPKTFAIVG
                     VGRSD"
BASE COUNT          845 a          346 c          441 g          677 t
ORIGIN      
        1 tggcccgaca atatgatatg ttacatgccg gaagaaaaaa ttttattttc aaacgattca
       61 tttggacaac attatgcaac atcgggtaga ttcgacgatg aagtatctca tgatgaagca
      121 ttatatgaag caaaaaaata ttttgccaat atcgttcttc cttactcggc tcaagtaatt
      181 aaagaacttg aagcagcttc aaaatttgaa atagaactca tttgcacaag tcatggtgta
      241 atttggaaaa aatatattgc agaaataatt caagaataca ataaatgggc tcgtaacgtt
      301 accaaaaaga aagttgtaat tgcatatgat actatgtgga aaagtaccga aaaaatggca
      361 tatgctatat atgaagcttt tgaacaagaa ggatatgaaa tagcattacg caatttacaa
      421 gtaaatcatg aatcagatgt aatgaccgac gtaattgacg cagaatatat ttgtgttggt
      481 tcgcctacac ttaattctga aatgatgaca aacgtagttg gatttttaac ttacatgcgt
      541 ggacttgctc ctaaaggcgg acgaaaagct gtggcttttg gttcatatgg ttggaatggt
      601 aaaagcatac ccggtgttga acaattctta aaagattgca attacgatgt taaagcggta
      661 tttacacacc agtatagacc aaccaaagaa gatttacaac aaataacaac aaaaactcta
      721 gaaataataa aacaaaacaa ttaaaagcta tgacaaaacg aagtgaaatc tatcaatgtg
      781 gcttatgtgg taatatagta gaagtattac atcaagctcc cggaaaatta gtttgctgtg
      841 gtcaatcaat gaatttactt gaagaaaatt caaaagatgc tgcaacagaa aaacatgtac
      901 ctgtaattac taaaattgaa ggtggataca aagttaccgt aggtgaagta ttacacccta
      961 tgcaaccaga acactatatt gaatggatag aacttattgc aggcaatgaa atattcagaa
     1021 aatatttgca accgggcgaa actcccgaag ctatttttta cactcaagct acagatgtat
     1081 atgctcgaga attttgtaat ctacatggca attggaaatc gtaataatta tcaaaaaaaa
     1141 tataatcccc tttgttttta acactgggga ttttttttat atttatacat gaaaaaaact
     1201 gttactttgt gtaatgtttt tatcagtaac tcacactaat accaaaaata aaaaaatatc
     1261 acatgaaaat aggatttata ggattaggta aaatgggcgg gaacatggtt caacgccttt
     1321 tgttagacaa acatgaagta gtagtatttg actttaatac ttctattgtg aaagatttag
     1381 aaaaaaaagg agcaattgct gctaccgaca ttgaagacat gattcaaaaa ttacccgata
     1441 ctaaagttgt atggcttatg gtaccggcag gtaaacccgt agatgatacc ataaccaaac
     1501 ttttaccttt gttacaaaaa ggtgatattc ttattgacgg tggaaattca ttttggaaag
     1561 attctcaagc tcgggcacaa cgcactgcat tcaaaagtgt agaatttgta gattgtggta
     1621 caagtggcgg aatatgggga ttacaaaacg gatactgctt aatgtatggt ggaaataaac
     1681 aaacctgtga tagtttggaa ccaattttca aaagtttagc aacagacaac ggatatttat
     1741 attgtggcga atcaggtgcc ggacattttg ttaaaatggt tcacaatgga atagaatatg
     1801 gtatgatgca agcatttgct gaaggatttg aactgatgga aaaatcaccg tttacccctg
     1861 atttagagaa agttgccgaa ttgtggcgtc atggaagtgt agtacgctca tggttacttg
     1921 accttattaa aaatgcttta gatgaagatc caaaattaga gactattaca gactatgttg
     1981 acgacaatgg tgaagcacgt tggatgatac aatctgctat ggattttgaa gtaccggttc
     2041 atgttactgc agcatcatta tttactcgct ttgaatcacg tcaaaatgaa tcatttgcaa
     2101 tgaaaatgct tgcagctatg cgtaatcaat ttggtggtca tgccgtaaaa actaaataat
     2161 atgaattcaa aagacaaaca tattcttgta atatttggag gttcgggtga tcttacccaa
     2221 cgaaaactca tacctgcatt atacgattta catagaaaaa aaatgttgcc aaaaacattt
     2281 gctattgtag gtgtaggacg aagtgatat
//
LOCUS       DAOTOW010000358         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36321_length_2306_cov_2.946690, whole genome shotgun sequence.
ACCESSION   DAOTOW010000358 DAOTOW010000000
VERSION     DAOTOW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36321_length_2306_cov_2.946690"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..>2306
                     /locus_tag="P5243_06935"
     CDS             343..>2306
                     /locus_tag="P5243_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HRS19220.1"
                     /translation="MIGLLVIANTTFAQNPNELLNKWNAIVSNNITSVSEIEGRLFVG
                     NNYNVQNAHQYGFRLNSNPANDVVFTVGGTVTTNGQANIKVFYGSAAIAGSVSQTNWF
                     EFHNGGSLIQNSNYAAQNSPVAALTAASAYWATLTPNGTVSYPTNQPAPLRFTCPSGQ
                     ELAVFNITDVLSLENSKVQQIEIIPAAATKTVIINIASVDGTVNWNYGNMVGNLDNQN
                     FRSKVIWNIYTTANNGQLNSVNCMPNMKGALIAPGATVTGNSNFDGPVFCKNLAINSE
                     IHLASSNGWQGNAPEVPSVPTCTQSWSGSLGADETVCNYDATTFTATGTITKQPSNAK
                     AYLQVYWKLVAPTTQDTSTHYSSKWIYSDTTVAVSGNWPGILATDTFVQLKVGMRLYN
                     CDMEKLGNDVVKNYTWNTTVCPPPAANEADIKVEKTVSSDSVQNGSQITFTIKATNNG
                     PKDASNIVVNDQLASGLTYVSSSATVGAYNSSTGLWIIPALNNGQSATLSIVVKAEVT
                     SSQSGSLNLGNAAPYNLFVLENFSSPSSDVEGRMAVGNNVFMSNYSVGYNQPADNYAT
                     QDVFVVGGNLTFLSGSVYNGNLVYGGSTNLPINQVSVVGGVLRQDSVINFDSTSVALR
                     TLANQILLMPSTGSVTYEYGGMFLNGNNPIT"
BASE COUNT          814 a          330 c          418 g          744 t
ORIGIN      
        1 actattttat aaattttttc tatttttcgg taacatttaa ttttgattat atacggtaat
       61 gtgattaaaa gcacattgaa ctaaatataa ctactaaaac attttagccg ctgtatcaaa
      121 atgaaaaaat gtatcaatat aagaaatttt ggatattaaa aaattattaa aaaatgtcca
      181 taattataag ataattaaat aaatatgtta tatttacgtg gtctttttaa tgaggaataa
      241 agtgattagg tttggcacaa aaatttatat aaataaacta ttaaaaataa ataaaataat
      301 attatagagg atagattgaa aaatagcaaa atttttaatg ttttgatagg acttttggta
      361 attgcaaata ctacatttgc tcaaaaccca aatgaattgt tgaataaatg gaatgcgata
      421 gtttcaaata acattacaag tgttagcgaa attgaaggca gattgtttgt cggtaataat
      481 tataatgttc aaaatgcaca tcaatatgga ttcagattaa attcaaatcc tgcaaatgat
      541 gtagttttta cagttggtgg aacagtaact acaaatggac aagcaaatat taaagtgttt
      601 tacggaagtg ctgcaatagc gggttcggta agtcaaacta actggtttga attccataat
      661 gggggttcgt taatccaaaa cagtaactat gcagcacaaa attcacctgt tgccgcatta
      721 actgctgcat cggcttattg ggctacatta actcctaatg gtacagtaag ttatccaact
      781 aatcaaccgg caccattaag atttacatgc ccttccggtc aagaattagc tgtttttaat
      841 attactgatg tattaagttt agaaaattca aaagtacaac aaattgaaat aattcctgct
      901 gctgcaacaa aaacagtaat aattaatata gcttctgttg atggtactgt taattggaat
      961 tacggaaata tggttggcaa ccttgataat caaaatttta gaagcaaagt tatctggaat
     1021 atttatacaa ctgcaaataa tggtcaattg aatagtgtaa actgtatgcc taatatgaag
     1081 ggggcgttaa ttgcaccggg tgcaacagtt accggaaact caaactttga tggaccggtt
     1141 ttttgtaaaa atttagctat taatagtgaa atacatttag cttcatcaaa cggttggcag
     1201 gggaatgccc cggaagttcc aagtgtgcct acttgtactc aatcatggag tggttcttta
     1261 ggtgctgatg aaacagtttg taattatgac gcaactactt ttacggcaac tggaactata
     1321 actaaacagc cttcaaatgc aaaagcgtat ttacaagttt attggaaact tgtagctcct
     1381 acaactcaag atacaagtac tcattactca agtaaatgga tttactctga tactacagtt
     1441 gccgtaagtg gcaattggcc cggaatactt gcaacagata cttttgttca gttaaaagtt
     1501 ggaatgcgtt tatataattg tgatatggaa aaacttggaa acgatgtagt taaaaattat
     1561 acatggaaca ctaccgtatg tccgcctccc gcagcaaacg aagctgatat taaagttgaa
     1621 aaaacagtat caagcgatag tgttcaaaat ggttcgcaaa tcacatttac aattaaagca
     1681 acaaataatg gtcctaaaga tgcatctaat atagttgtaa acgatcaatt agcatcggga
     1741 ttaacatatg ttagtagttc ggcaactgtt ggagcatata attcaagtac gggattatgg
     1801 attattcccg cattaaataa tggacaatcg gcaactttat caatagttgt taaagccgaa
     1861 gttacttcaa gtcaatcagg ttcattaaat ttgggaaatg cagcacctta taatttgttt
     1921 gttttagaaa acttcagcag tccaagttcc gatgttgaag gaagaatggc agttggaaat
     1981 aatgttttta tgagtaatta tagtgtaggt tacaatcaac ctgctgataa ctatgcaact
     2041 caagatgttt ttgttgttgg tggcaattta acatttttat caggttcggt atataatgga
     2101 aatttagttt atggtggaag cacaaattta cctataaacc aagtttcggt tgttggtgga
     2161 gtattaagac aagattcggt aattaatttt gattctacat cagttgcatt acgtactttg
     2221 gcaaatcaaa tattacttat gccttctacc ggttcggtaa cctatgaata tggtggaatg
     2281 tttttgaatg gaaacaaccc tattac
//
LOCUS       DAOTOW010000359         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36360_length_2304_cov_3.302801, whole genome shotgun sequence.
ACCESSION   DAOTOW010000359 DAOTOW010000000
VERSION     DAOTOW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36360_length_2304_cov_3.302801"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            576..1490
                     /locus_tag="P5243_06940"
     CDS             576..1490
                     /locus_tag="P5243_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRS19221.1"
                     /translation="MKKKLLHTIEALSSPQSYNSVYHEPNHGSPMPSIKELKEIVATI
                     REIIFPGYFGLEILQEETISFYTGVNIDKLFNLLTRQIRRGLCFGCDTNTITHTCNTC
                     DNQAEELALQFIETLPNIRRLLATDIQAAYNGDPAAKSFGEIIFCYPVIKALTNYRVA
                     HELVKLNVPLIPRIISEMAHSETGIDIHPEATIGEYFTIDHGTGVVIGATSIIGNHVQ
                     LYQGVTLGAKNFPLDEHGNPIKGVPRHPIVEDNVVIYSNATILGRITIGTGAIIGGNV
                     WVTNDVPAGARILQSRPRSESFEYGGGI"
     gene            1510..1935
                     /locus_tag="P5243_06945"
     CDS             1510..1935
                     /locus_tag="P5243_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRS19222.1"
                     /translation="MYAYILLFIWCFTASSIIPVSSEPYFTTLIITKQVWLPALIIAS
                     IGNILGSITTYWLGKKAGEIGIHKLHEKHKKRVIQAQHIVHKYGTASLFFAWVPIIGD
                     IIVTIAGTLQTPLVRSIIWISAGKIFRYSLITAITLHFI"
     gene            2119..>2304
                     /locus_tag="P5243_06950"
     CDS             2119..>2304
                     /locus_tag="P5243_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19223.1"
                     /translation="MPAHKSKNFRFRIIDECLQNTSRTWTLEDLIEEVSNKLTDEFGV
                     QKVSKRSIQYDIALMREK"
BASE COUNT          850 a          422 c          354 g          678 t
ORIGIN      
        1 aatcacaaca aggaacaaac attgttacaa tcacaggagt tgtttatact atgtcaaaga
       61 tacaaaatga accgtaacga agtggagctc ggcgaagcca aaccaaatca caacttttca
      121 tagtagtata ttttaattgt tatgttgttt atactatgtc aaagatatcc gccgcggcgg
      181 aaaaccaaat catcccgatt tagaatttct attgtatctc aaaaaaaaaa gttaactcca
      241 aaattttaag aaattcttta tcagaatcaa agatacaaaa tgaaccgtaa tagcttgtcc
      301 cgatgataat ttcgggaaag tggagctcgg cgaagccaaa ccaaatcaca attttttctt
      361 tttcaaaatc attcttttga atttgaatgt ttacttatat agtatttgca atataaacct
      421 taacatatac ccaaaccacc ttgaacttgg tattaaattg cacgactcac atacccactt
      481 tctaataata aagataaaaa aaatcattta aaactattca tctcccacag aatcttagta
      541 ttattgtaaa taatttgtat caaattttga atccaatgaa gaaaaaatta ttacatacta
      601 ttgaggcact ctcatcacca caatcataca attcggtata tcacgaaccc aaccacgggt
      661 cgcctatgcc atcaataaaa gaattgaaag agatagttgc aactattaga gaaattattt
      721 ttcccggata ttttggactt gaaattttac aagaagaaac tatttctttc tataccggag
      781 taaacataga taaattattt aatttactaa ctcgtcaaat acgtcgcgga ttatgctttg
      841 ggtgcgacac aaatacaatt actcacacat gcaatacctg cgataaccaa gcagaagaat
      901 tggcattaca atttattgaa acattaccaa acattcgcag actattagcc accgatattc
      961 aagcagcata taacggtgac cctgcggcaa aatcatttgg tgaaattatt ttttgttatc
     1021 cggtaataaa agcacttaca aattaccgag tagcgcacga attagtaaaa ctcaatgttc
     1081 cgcttattcc tcgaataatt tcagaaatgg cacactcaga aaccggtata gacattcacc
     1141 ccgaggcaac aattggcgaa tattttacca tagaccacgg aacaggtgtt gtaataggtg
     1201 caacaagcat tataggcaat catgttcaat tgtatcaagg ggtaacatta ggagctaaaa
     1261 attttccgct tgacgaacac ggcaatccaa taaaaggagt tcctcgtcac ccaatagttg
     1321 aagacaatgt tgtaatatat tctaacgcta ccatattggg acgcataacc ataggaaccg
     1381 gtgctattat aggcggtaac gtatgggtaa ccaacgatgt acctgcaggt gccagaatat
     1441 tacaatcgcg cccccgttct gaatcatttg aatatggcgg cggcatataa catatttaat
     1501 ctataaaata tgtatgccta cattctgtta ttcatatggt gttttacagc ttcaagtatt
     1561 atacccgtat catcagaacc atattttacc acccttataa ttaccaaaca ggtatggtta
     1621 ccggctctta ttatagcaag tattggtaac atattgggta gcattacaac ctattggtta
     1681 ggaaaaaaag caggtgaaat aggaatacac aaactacatg aaaaacataa aaaacgtgta
     1741 atacaggcac aacacatagt tcacaaatac ggcacagcaa gtttattttt tgcatgggtt
     1801 cctataattg gagatattat agttacaatt gcaggaactt tacaaacacc tcttgtgcga
     1861 agcattatat ggataagtgc aggaaaaata tttcgataca gtttaataac tgcaataaca
     1921 ttgcatttta tttaaaatat taaataataa tacattaaca catcaaaata acatacctaa
     1981 acacacaaac aaagtgttta gaatacaata aatctacaac tttttttcat aaaaattgaa
     2041 aataatttgc aaaatacttg cattgcattt attttgcatt tacttttgct ttaattatta
     2101 atctgttaga atactattat gcctgctcat aagtcaaaaa actttcgatt tcgcattatt
     2161 gacgaatgct tacagaatac aagtcgcaca tggacgcttg aagatttaat agaagaagtt
     2221 tctaataaac taaccgatga gtttggtgta cagaaggtaa gcaaacgctc gatacaatac
     2281 gacatagcac ttatgcgaga aaaa
//
LOCUS       DAOTOW010000360         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36402_length_2302_cov_3.350245, whole genome shotgun sequence.
ACCESSION   DAOTOW010000360 DAOTOW010000000
VERSION     DAOTOW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36402_length_2302_cov_3.350245"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="P5243_06955"
     CDS             complement(<1..1380)
                     /locus_tag="P5243_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS19224.1"
                     /translation="MSEENKNQKEYSADSIQVLEGLEAVRKRPAMYIGDIGEKGLHHL
                     VYEVVDNSIDEALAGYCTDIDVFINEDNSITVRDNGRGIPVGWHEKEQKSALEVVMTV
                     LHAGGKFDKDSYKVSGGLHGVGVSCVNALSTKCVVTVNSKDGKVYQQTYCIGVPQGPV
                     EVIGTTTTTGTEVQFWPDGSIFMSTVYKFNILESRLRELAYLNKKVSLRITDKREKDE
                     NGEYKVSHFYSEEGLKEFARFADETREPLIEDVLYIDTEKNGIPVEIALKYNTSYAEN
                     IHSYVNNINTIEGGTHLLGFRRGLTRTLKKYAEDSGILAKQKIEINGDDFREGLTAII
                     SVKVQEPLFEGQTKTKLGNSEVSAAVDQAVSEMLKYYLEEHPKEAKTIVNKVLLAASA
                     RIAARNARERVQRKNSMSGGGLPGKLADCADRDPAKCELYLVEGDSAGGTAKQGRNRA
                     FQAILPLRGK"
     gene            1613..2101
                     /locus_tag="P5243_06960"
     CDS             1613..2101
                     /locus_tag="P5243_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRS19225.1"
                     /translation="MNKRILPADKALEKLMAYCSKMERCEFDVKQKLYNWGIDDKKSL
                     AIVDKLKKEGFINNDRYVESYIRGKYYYNKWGRVKIRFNLKLKGYDENLIAPALDSFF
                     DNVDYEKMIYEQLERKNRSLTIEDEYQRKSKLIQFGQSRGYETEISLSCVDSIVSSES
                     NE"
     gene            2117..>2302
                     /locus_tag="P5243_06965"
     CDS             2117..>2302
                     /locus_tag="P5243_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19226.1"
                     /translation="MINTLQHIFTKQYNILVVIVLCVFMFSCKDKTPQPIVNNQKIQY
                     VILTPPYQSNFLFTQITI"
BASE COUNT          767 a          440 c          305 g          790 t
ORIGIN      
        1 tttaccgcga agtggtaaaa ttgcttgaaa agctctgttt cgtccttgtt tagcagtacc
       61 tcccgcagaa tcaccctcaa ctaaatataa ttcacatttt gcaggatctc tatcggcaca
      121 atcggctagt ttgcccggca aacctccacc gctcatagag tttttacgtt gcactcgctc
      181 acgtgcatta cgggctgcaa tacgtgccga agcagcaagt aaaactttat ttacaattgt
      241 tttagcttct ttaggatgtt cttctaaata atacttaagc atttcgctta cagcttggtc
      301 taccgcagca cttacctcag agttacccaa tttagttttg gtttgtcctt caaacaatgg
      361 ctcttgaact tttacggaaa taatagcagt caacccttca cgaaaatcat ctccatttat
      421 ttcaattttt tgttttgcaa gaattcctga atcttcagca tattttttta aagtacgcgt
      481 aagacctctc ctaaatccta ataaatgagt acctccttca atagtattaa tattgtttac
      541 gtatgagtga atattttcgg catacgacgt attatatttc aatgctattt ctacaggaat
      601 tccattcttt tcagtgtcta tatacaaaac atcttcaata agtggctcac gtgtttcatc
      661 ggcaaaacga gcgaattctt tgagtccttc ttctgaataa aaatgagaaa ctttgtattc
      721 tccattttca tctttttctc gcttatctgt aattcgcaat gaaacttttt tgttcaaata
      781 tgccaattca cgcaatcgcg attctaatat attaaactta taaacggttg acataaaaat
      841 acttccatca ggccaaaact gaacctcagt tcctgtagtg gttgttgttc caattacttc
      901 aaccggacct tgcggcactc ctatacaata tgtttgttga tataccttac catctttact
      961 atttacagtc acaacacatt ttgtagataa cgcattcaca catgatacac caacaccatg
     1021 taatccacct gatactttat atgaatcctt atcaaattta ccaccggcat gtaaaacagt
     1081 cataacaact tccaatgcag atttttgttc tttctcgtgc caacctacag gaatacctct
     1141 accattatct cttaccgtaa tagaattatc ttcatttata aatacatcta tatccgtaca
     1201 atatccggcc aaagcctcat ctatagaatt atcaaccacc tcatacacca gatgatgcaa
     1261 gcctttttct ccaatatctc ctatatacat agccggtctt ttacgaacag cctctaaacc
     1321 ttctaatacc tgaatactgt cagcggaata ttctttttga tttttatttt cctcactcat
     1381 ttatatatat ctgattttcc gttagttata ctttattttc aatcacagtt ttattcgaac
     1441 gaatgtacaa aaaactattt tcatttaacc aaaaaaatac tatgaaagaa ttaataactt
     1501 atcaacaaca cacatgtcat aataagatat ttttttcatt tttataatag tgtattgttt
     1561 ttttaaaata agtatatatt tttaccctac gtaataatta atatgtagta ctatgaataa
     1621 acgtatttta cctgccgata aagctttgga aaaattgatg gcatattgca gcaaaatgga
     1681 acgatgcgag tttgatgtaa aacaaaaatt atataactgg gggattgatg ataaaaaatc
     1741 tcttgctatt gttgataaac tcaagaaaga aggttttata aacaacgaca gatacgtaga
     1801 atcatatatt cgaggtaaat attattacaa taagtgggga cgagtaaaaa ttcgttttaa
     1861 tcttaaacta aaaggatacg atgaaaatct cattgctcct gcattagatt cattttttga
     1921 caatgttgac tatgaaaaaa tgatatatga acaattggaa cgtaaaaatc gttctttaac
     1981 aatagaagat gaatatcaac gaaaatctaa attaatccaa tttggacaaa gtcgcggata
     2041 tgaaaccgaa atttctcttt cttgtgttga tagtattgta agttctgaaa gtaacgaata
     2101 atagtaccct aataaaatga ttaacacatt acaacatata tttaccaaac aatataatat
     2161 acttgttgta attgtattgt gtgtattcat gttctcatgt aaagataaaa cacctcaacc
     2221 tatagtaaat aatcaaaaaa tacaatatgt aattctaact ccgccgtatc aaagtaattt
     2281 tttgtttaca caaataacaa ta
//
LOCUS       DAOTOW010000361         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36403_length_2302_cov_3.345349, whole genome shotgun sequence.
ACCESSION   DAOTOW010000361 DAOTOW010000000
VERSION     DAOTOW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36403_length_2302_cov_3.345349"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1174
                     /gene="tsaD"
                     /locus_tag="P5243_06970"
     CDS             134..1174
                     /gene="tsaD"
                     /locus_tag="P5243_06970"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRS19227.1"
                     /translation="MNITILAIESSCDDTSAAVIQNGLILSNIIANQSVHQEYGGVVP
                     ELASRSHQQNIVPVVEKALKQAGVSKQDITAVAFTRGPGLLGSLLVGTSFAKGFALAH
                     QCKLIEVNHLHAHVLASFIQTPTERIAEPQFPFLSLLVSGGHTQIIIAHSYTNMEIIG
                     TTIDDAAGEAFDKCAKVLGLPYPGGPHIDNHAQKGNPLAYSFNKPSIPDFDFSFSGLK
                     TSVLYFLRDQIKQNPEFISQNIDDICASLQKTIIDILMQKLHAAVKHTGIREIAIGGG
                     VAANSGLRNAIAEFGKKYGYNTYIPERKYTTDNAAMIAITAYHKYLAQDFASQDISPR
                     ARYSLQEYFGKI"
     gene            complement(1272..>2302)
                     /locus_tag="P5243_06975"
     CDS             complement(1272..>2302)
                     /locus_tag="P5243_06975"
                     /inference="COORDINATES: protein motif:HMM:NF006388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS19228.1"
                     /translation="GKPELRALWKQFIYKKNPSLGTQPISLPIATNGLTHAISMAAYM
                     FANAGDTILIPDLFWENYDLIFNKSYGATISTFNSFANNGFDVDAFSNAMNNAPGNKI
                     ITLLNFPNNPTGYTPTVKEAEQIQQVVLAQAQKGKQVIVIIDDAYFGLVYEDGIFMES
                     IFTKLCNLHTNVLAVKLDGPTKEDYVWGFRVGFMTYGIKEGSAALYEALEAKTAGAVR
                     GNISNISNLSQSLLYKVYQSPKYDEYKQEKYSILKSRYQVLSKALKANNLGKYYDSLP
                     YNSGYFMCIRLKPGIDGEKLRQLLLEKYSTGVIVFGDIVRLAYSAVPEAKIETLVSNI
                     YAACSELA"
BASE COUNT          787 a          428 c          345 g          742 t
ORIGIN      
        1 aaaccaatca ttgtttttac taatatgttc actatggtac gtatgatatt caatttttta
       61 aaacaaaata aataaattta accgtatttt tgcacaaata taatgattta cgcatgtttc
      121 aaactataca tacatgaata ttacaatact tgccatagaa tcatcgtgcg acgatacttc
      181 ggcagcagta atccaaaacg gattaatttt gtcgaatatt atagcaaacc aaagtgtaca
      241 tcaagaatat ggaggtgtag ttccggaatt agcttcgcgt tcgcaccagc aaaatatagt
      301 acctgtagtt gaaaaagcat taaaacaagc cggagttagc aaacaagata ttaccgctgt
      361 agcatttact cgtggcccgg gcttacttgg ttcgctcttg gttggtacat cgtttgcaaa
      421 aggatttgcc ttggcacatc aatgtaaact tatagaagta aatcatttac atgcacatgt
      481 attggcaagt tttattcaaa ctccaaccga acgcatagct gaaccacaat ttccttttct
      541 gtcattattg gtatcagggg gacatacaca aataattata gcccatagtt acaccaatat
      601 ggaaatcata ggcactacaa tagacgacgc tgccggcgaa gcatttgaca aatgtgccaa
      661 agttttgggg ctcccctatc ccggcggacc acacattgat aatcatgcac aaaaaggaaa
      721 tccgttagct tattcattta acaagccttc tatacctgat tttgatttta gttttagcgg
      781 cctcaaaaca tcggttttat atttcttacg cgaccaaatc aagcaaaatc ccgaatttat
      841 ttcgcagaat attgatgata tttgtgcttc actacaaaaa actattattg atattttaat
      901 gcaaaaacta catgcagcag taaaacatac cggcatacgc gaaatagcta taggcggtgg
      961 tgttgctgca aattcaggat tacgcaatgc cattgccgaa tttggaaaaa aatatggtta
     1021 caatacatat attcctgaac gaaaatatac tacagataat gctgcaatga tagccattac
     1081 agcatatcat aaatatttag cacaagattt tgcctctcaa gacatttcac cacgagctcg
     1141 gtattcatta caagaatatt ttggaaaaat ataattattc ttaatatctc ataaaaaaaa
     1201 aggtaatcac gcatgattac ctttttttat accttttata ttcaactcta ataataagaa
     1261 aacctattat attaagctaa ttctgaacaa gcagcatata tatttgaaac caaagtttca
     1321 atttttgctt cgggtaccgc agaatatgcc aatcgaacaa tatcgccaaa tacaattact
     1381 ccggtagaat atttttcgag caataattga cgtaattttt ctccatcaat accgggtttc
     1441 aaccgaatac acataaaata tcccgaatta tatggcaaac tgtcgtaata ttttcctaaa
     1501 ttatttgctt tcaatgcttt tgaaagaact tgataacgcg actttaaaat agaatacttc
     1561 tcttgtttat attcatcgta ttttggtgat tgatatactt tatataacaa tgattgcgat
     1621 aaattgctaa tattagatat gttaccacga acagcacctg cagttttagc ttcaagagct
     1681 tcatataatg ctgcagaacc ttctttgatg ccataagtca taaacccaac tctaaacccc
     1741 catacatagt cttctttagt tggtccatct aattttacag ccaacacatt ggtatgcaaa
     1801 ttacacaatt tggtaaaaat tgactccata aaaataccat cttcatatac caatccaaaa
     1861 taagcatcgt ctattataac tattacttgt tttccttttt gtgcttgagc caatacaact
     1921 tgttgaattt gttctgcttc ttttactgta ggtgtatatc cggtaggatt attaggaaag
     1981 ttaagtaatg taataatttt attacccgga gcattgttca tagcatttga aaatgcgtca
     2041 acatcaaatc cattatttgc aaatgaatta aatgtagaaa ttgttgcacc gtatgattta
     2101 ttaaaaatta aatcataatt ttcccaaaac aaatcaggaa tcaaaattgt atcacctgca
     2161 ttagcaaaca tataagcagc catacttata gcatgtgtca aaccattggt tgctataggc
     2221 aaactaatag gttgtgtacc taacgatgga ttttttttgt atataaactg tttccacaat
     2281 gcacgtagtt caggttttcc aa
//
LOCUS       DAOTOW010000362         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36487_length_2298_cov_3.126170, whole genome shotgun sequence.
ACCESSION   DAOTOW010000362 DAOTOW010000000
VERSION     DAOTOW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36487_length_2298_cov_3.126170"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2298)
                     /locus_tag="P5243_06980"
     CDS             complement(<1..>2298)
                     /locus_tag="P5243_06980"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HRS19229.1"
                     /translation="KHIGTEQITILRSAVTGYGEDLLHAAFNIDDGIVETIAHTTAAQ
                     KINPDVSFILDIGGQDMKAIFIKNGAIQSIEINEACSSGCGSFIQNFAQTLSYSAQDF
                     GTLACSAQNPCDLGSRCTVFMNSQVKQSLKEGAEISEIAAGLAYSVIKNALNKVLQIK
                     DMNVLGNAIMVQGGTFKNPSVFRAMEVITQKPIYTTNFPELMGALGAAYYSYEIWTKN
                     PTDSCCISYENRYNALEYDSRQITCKGCNNNCIVTKFTFNKDNIHYSGNKCEKVFSSK
                     GQYKERGENIYEYKYDILFNREQQALYNKLPLTIGIPRILGMYSNYPFWNTLFTKSGI
                     SVQLSDESTTKIFEKGQGTIMADNICFPAKLAHGHVLNLVEKQVDRIFYPLVIYEENE
                     HPEAKNSYNCPIVSSYSEVLKSSIPKNKRNNIPIDKPVISFRDEHLLKKNCWQYLKKL
                     GVSRFTFERAFKDALLEQKNYKEQYRLKGFRILERAKLDKKPIIMLAGRPYHTDPLVQ
                     QKTADILCELGASVISEEIMLHIEGTSLTDYLHVAQWNYPNRILKAAQWVIHNTEYDV
                     HFVQINSFGCGPDSFITDELKELFEKSGKSFTFIRVDEINSTGSMRLRLRSLLSTIEL
                     DSKIASSDSAKITRTPPFTEHDKKKHIIGPFFTRFHSPFIAPAFELMGYSMETLPVSN
                     KESVEVGLRYANNEICYPATLIVGDIVKALQSGKYDLNNTAVVMSQTGGQCRASNYLA
                     LIRKSILHAGIDTVPVISLATAGNGV"
BASE COUNT          622 a          423 c          453 g          800 t
ORIGIN      
        1 accccattgc ctgctgttgc caatgaaatt accggcacgg tgtctatacc cgcatggagt
       61 atagatttgc gtatcaatgc taaatagttc gaggcacggc attgccctcc tgtttgcgac
      121 attactactg cggtattgtt gaggtcgtat ttgcccgatt gcagtgcttt tactatatca
      181 ccaacaataa gggtagcagg atagcatatt tcattatttg catatcgtaa tcctacttct
      241 accgattctt tgtttgacac aggtaatgtt tccatgctat agcccataag ttcaaacgca
      301 ggtgctataa atggtgagtg aaaacgagtg aaaaatggac caataatatg ttttttctta
      361 tcgtgttcgg taaatggggg agttctcgta atttttgccg aatcggacga tgcaattttt
      421 gaatcaagtt caatagtaga aagtaacgaa cgcaaccgta accgcatact gccggtacta
      481 ttaatttcgt caactcgaat gaatgtaaat gattttcccg atttttcgaa caattccttc
      541 aattcatcgg ttataaagct gtcgggacca caaccaaatg aatttatttg cacaaagtgt
      601 acatcgtatt cggtgttgtg aattacccat tgtgctgctt ttaaaattct gtttggataa
      661 ttccattgag ctacatgcaa atagtcggta agcgatgtgc cttctatatg aagcataatt
      721 tcttcgctaa ttaccgaagc tcccaattcg cagagtatat ccgcagtttt ttgttgtacc
      781 aaggggtcgg tatggtaggg ccttccggca agcattatga ttggtttttt gtccaatttt
      841 gctcgttcca atatgcgaaa tcctttgaga cggtattgct ctttgtaatt cttttgttca
      901 agtagagcgt ctttaaatgc ccgttcaaat gtaaaacgcg atactcctaa ttttttgaga
      961 tactgccagc aatttttctt aagcagatgt tcgtctctaa acgaaattac cggcttgtct
     1021 ataggtatat tgttgcgttt gtttttaggg atagaacttt tgagtacttc cgaatagctt
     1081 gaaactattg gacaattata gctgttttta gcctcggggt gttcgttttc ttcataaatt
     1141 acaagagggt aaaaaattcg gtcaacttgt ttttcaacta aattcaatac atgtccgtgt
     1201 gctaatttcg cggggaaaca aatattgtcg gccattatag tgccttgtcc tttttcaaat
     1261 attttagtag tagattcgtc tgacaattgt accgaaatgc ccgatttggt gaacaatgta
     1321 ttccaaaacg gataattgga gtacataccc aatattcttg gtataccaat agtaagaggt
     1381 agtttgttat atagagcttg ttgttcgcgg ttaaataata tgtcatattt gtattcataa
     1441 atattttcgc cacgttcttt gtattgccct ttggaactaa atactttttc gcatttattt
     1501 cccgaatagt gaatgttgtc tttgttaaat gtaaattttg ttacaataca attgttatta
     1561 caacctttgc aggtaatttg tcggctatcg tattctaatg cattgtaacg gttttcgtac
     1621 gaaatgcagc atgagtctgt tgggttttta gtccatattt cgtaactgta atacgcagca
     1681 cccaaagcac ccataagttc aggaaaattg gtagtatata taggtttttg tgtgataacc
     1741 tccattgctc gaaaaaccga tggatttttg aatgtgccgc cttgaaccat aatcgcattg
     1801 cccaacacgt tcatgtcttt gatttgcaac actttgttta atgcattttt tataacagaa
     1861 tatgccaatc ctgcggcaat ttcagatatt tctgcccctt ctttgagcga ttgttttact
     1921 tgtgaattca taaaaacagt gcatcgcgac cctaaatcac aagggttttg tgcagagcag
     1981 gcaagtgtac cgaaatcttg tgccgaatag gagagggttt gcgcaaaatt ttgaataaac
     2041 gatccacagc ccgaagaaca tgcttcgttg atttcgatag attgaatagc accatttttt
     2101 ataaaaattg ctttcatatc ttgtccgcca atatcgagta taaacgatac gtcgggatta
     2161 attttttgtg ctgcagtagt gtgtgcgata gtttctacta tgccatcgtc tatattaaat
     2221 gctgcgtgaa gtaaatcttc gccatagcct gtaactgccg agcggagtat tgttatttgt
     2281 tcggttccta tatgtttt
//
LOCUS       DAOTOW010000363         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36512_length_2297_cov_2.931757, whole genome shotgun sequence.
ACCESSION   DAOTOW010000363 DAOTOW010000000
VERSION     DAOTOW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36512_length_2297_cov_2.931757"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="P5243_06985"
     CDS             <1..1818
                     /locus_tag="P5243_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRS19230.1"
                     /translation="KITPAHDENDYKIGMEHNLEVIDIFNDNGTLNKKAQLYVGKDRF
                     AVRTEIVADLQNAGFIVKIEDYTNKVGYSERTHVVIEPKLSTQWFLKMKEITAPALAN
                     VMNDEIEFFPKKFKNVYRHWMENIKDWCISRQLWWGHQIPVYYLPDGSHVCAATIEEA
                     LQLAQQKTGNTSLTIADLRQDEDVLDTWASSWLWPISVFGGITNPNNKEINYYYPTNV
                     LVTGHDIIFFWVARMIMTGYEYRKTYPFKHVYFTGMVRDKLRRKMSKSLGNSPEPLEL
                     IEKYGADGVRVGMLLCSPAGGDLLYDDALPEQGRNFSSKIWNAFRLVQGWNVDNTIEQ
                     PAYAKAAIEALTEKIKEASIQLDEQFEQYRLSEAMMTVYKLFWDELSAWYLEIVKPEY
                     GKPIDGATYTTTIQLFESILQLLHPFMPFITEEVWHYLKPRGTGESIMISQIPTYSSC
                     NTDLLVRYERIKEVVAGIRGIRKDKQVPFKETIELHIKGIDAPQDFEFVILAKLGNIS
                     AIHITNQDISNAASFRVDAIEYFIPLAQSINVEEEKQKIQEELTYIQGFLASVDKKLM
                     NEKFVSSAPAAVVETERKKKADALEKIELLEKRLKTIQG"
     gene            1922..2281
                     /locus_tag="P5243_06990"
     CDS             1922..2281
                     /locus_tag="P5243_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19231.1"
                     /translation="MKTFFLKIIKALLISISISITAITAQTNTSKSTIPSARQIIAAI
                     SHDSLQGRKPATAGYDKAARYVEQQLLTIGAKPLFGTLFIDSVSTYYGYNVVGVLESP
                     HSLNEYMSSFTRLFMIT"
BASE COUNT          796 a          377 c          454 g          670 t
ORIGIN      
        1 aaaattactc ctgctcacga cgaaaacgac tataaaatag gcatggagca caacttagag
       61 gtaattgata ttttcaatga caatggtaca ttaaacaaaa aagcacaatt gtatgtaggt
      121 aaagaccgtt ttgctgttcg taccgaaatt gttgccgatt tgcaaaatgc aggatttatt
      181 gtaaaaattg aagattacac taataaagta ggttattcgg aacgaaccca tgtagtaata
      241 gagccaaaac tttcgacaca gtggttttta aaaatgaaag aaattactgc tccggctttg
      301 gcaaatgtaa tgaacgacga aattgagttt tttccgaaaa agttcaaaaa tgtgtatcgc
      361 cactggatgg aaaatataaa agactggtgt attagccgtc agttgtggtg gggacaccaa
      421 attccggtgt attatttacc cgatgggtcg cacgtatgcg ctgcaactat tgaagaggca
      481 ttacaacttg cgcaacaaaa aacaggcaat acttcattaa ctattgcaga tttacgtcaa
      541 gacgaagatg tattggatac gtgggcatcg tcgtggttgt ggcctatttc ggtatttggc
      601 ggtattacca atcccaacaa caaagaaata aattattatt atcctaccaa tgtattggtt
      661 accggacacg atattatttt cttttgggtt gctcgtatga ttatgaccgg ttatgaatat
      721 cgcaaaacgt atccgttcaa acatgtatat tttaccggta tggtacgcga taaattgcgc
      781 cgtaagatga gtaaatcttt gggaaattcg cccgaaccat tggagttgat agaaaaatac
      841 ggagccgatg gtgtacgtgt aggtatgctt ttgtgttctc ctgccggagg cgacttgttg
      901 tacgatgatg cgttgcccga acaaggacga aatttctcga gtaaaatatg gaatgcgttc
      961 cgtttagtgc agggttggaa tgttgacaat accatagaac aacctgccta tgcaaaagct
     1021 gcgattgaag cgttgaccga aaaaataaaa gaagcaagta tacaactcga cgaacaattt
     1081 gaacaatacc gtttgtcgga agctatgatg actgtgtaca aattgttttg ggacgaactt
     1141 tcggcttggt atttagaaat tgtgaaacca gaatatggta aaccaattga cggtgctacc
     1201 tatactacca ccattcaatt gtttgaatcg atactgcaat tgctgcatcc atttatgccg
     1261 tttataaccg aagaagtatg gcattatttg aaaccacgcg gcacgggaga aagtattatg
     1321 atttcccaaa ttccaacata ttcatcgtgt aataccgatt tgcttgttcg atatgaacga
     1381 atcaaagagg tagtggcggg tattcgtggt attcgcaaag acaaacaagt gccttttaaa
     1441 gaaacaatag aattacatat aaaagggata gatgctccac aagattttga atttgtaata
     1501 cttgcaaaac ttggaaatat ttcggcaatt cacattacaa atcaagatat ttctaatgca
     1561 gcatcgttcc gtgttgatgc aatagaatat tttattccac ttgcccagtc gataaatgta
     1621 gaagaagaaa aacaaaaaat acaagaagaa cttacctaca tacaagggtt tttggcttcg
     1681 gttgacaaaa aattgatgaa cgaaaaattt gtgtcgagtg cacctgctgc tgtagttgaa
     1741 acagaacgta agaaaaaagc cgatgcatta gaaaaaattg aattattgga aaaacgattg
     1801 aaaacaatac aaggataatg ttgtttatat aattacatag atttatctaa taaagaatta
     1861 tacatatcat tgtatttaaa gtatttttgt atataaaaca atcattataa atcaatatga
     1921 tatgaaaaca ttctttttaa agataattaa agcattactt ataagtatta gcatttcaat
     1981 tactgcaatt actgcacaaa caaatactag taaatctaca attccttcag cgcgacaaat
     2041 aattgctgcc atatcacacg attcgttaca agggagaaaa ccggcaactg cagggtacga
     2101 caaagctgct cgctatgtag aacaacaatt gcttactata ggtgcaaaac cattgtttgg
     2161 tactttattc atagatagtg taagtaccta ttacggctac aatgtagtag gggtattgga
     2221 gtcgccacac tctttaaatg aatatatgtc aagtttcaca aggttgttta tgataactta
     2281 attgcaaaga taaaact
//
LOCUS       DAOTOW010000364         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36583_length_2293_cov_3.193923, whole genome shotgun sequence.
ACCESSION   DAOTOW010000364 DAOTOW010000000
VERSION     DAOTOW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36583_length_2293_cov_3.193923"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="P5243_06995"
     CDS             <1..228
                     /locus_tag="P5243_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HRS19232.1"
                     /translation="ALQIKFAYAVTCHKAQGGQWNDVYIDHGYITENMMNLDFLRWLY
                     TAITRATDTVFLIHFDDKFFDTSTQKKKQKK"
     gene            314..703
                     /locus_tag="P5243_07000"
     CDS             314..703
                     /locus_tag="P5243_07000"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRS19233.1"
                     /translation="MKAIDIQQTDKTPKVEFDIDSKTFIIQGFSRPENIRDFYVPILH
                     SLSKELQTIVESSDKQKYSFHFQLEYFNSSSAKFISDIIKIIKQYVDQGLQATIYWHY
                     DKDDEEMKESGDDFSSISNISFEYIAE"
     gene            769..1929
                     /locus_tag="P5243_07005"
     CDS             769..1929
                     /locus_tag="P5243_07005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS19234.1"
                     /translation="MPKIVRILNRFNVGGPTYNVAYLTKYISSEYSTILLGGQKNESE
                     ASSEYILQELDVAYTIIPEMKRSIGIWNEIKALRKLIRIIKTEKPDIVHTHAAKAGTL
                     GRIAAWYCGVPYIFHTFHGHVFHSYFNPLTTKIFITIEQLLAKTCTAIVAISDIQKHE
                     IGTVFKICPPHKIEVIPLGFDLEKFTKDIQTKRLQFRQQYNLEHNEIAIGIVGRLVPV
                     KNHELFIQAFQKCLHISTKPIKAFIIGDGEMRHELESLWNTIEPGKNNSKLTFTSWIQ
                     DVDVAYAGLDIVCLTSHNEGTPVSLIEAQAAGKPIVSTNVGGIQNIVIEGKTALLTNN
                     TVEDFSNKLLQIIENEELQKQMSNHSQQFSLEKFGYKRLCKDMEHMYEKYIT"
     gene            2008..>2293
                     /locus_tag="P5243_07010"
     CDS             2008..>2293
                     /locus_tag="P5243_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRS19235.1"
                     /translation="MKLQALDFLIILLYIAATVFIGLYFKKRAEKNKSEYLLGGNTMR
                     WYMLGVSDASGMFDISGTMWLVTLMFVYGLKSIWIPWLWPTFNQIFLMMFI"
BASE COUNT          873 a          336 c          349 g          735 t
ORIGIN      
        1 gcattacaaa ttaaatttgc atacgctgtt acttgtcaca aagctcaagg cggacaatgg
       61 aatgacgtat atatagacca tggatatatt acagaaaata tgatgaattt agattttctc
      121 agatggctgt atactgcaat tacccgagca accgacactg tttttttaat acattttgac
      181 gataagtttt ttgatacatc tacacaaaag aaaaaacaaa aaaaataaat gtatgaaacc
      241 tttttttttg tatgtttgga aaaatgaaaa tacaatataa gtaatacaat aaaatgggaa
      301 cccatagttt attatgaaag caattgatat acaacaaacc gataaaactc caaaggttga
      361 gtttgatatc gattcaaaaa catttataat tcaaggattt tcgcgtcccg aaaatattcg
      421 agatttttat gtaccgattt tacactcttt atccaaagaa ttacaaacaa ttgtagaatc
      481 ttctgacaaa caaaaatact cttttcactt tcaattagaa tattttaatt cttcatcagc
      541 aaaatttata tcagatatta taaaaattat aaaacaatat gtagaccaag gattgcaagc
      601 aactatatat tggcactatg acaaagatga cgaagaaatg aaagaatccg gtgatgattt
      661 ctcttcaatc tcaaacattt cgtttgaata cattgcagaa taaatagact caattatata
      721 ttactatatc atttttttat acttttgcgg cacaaatatg aggaatgtat gccaaaaatt
      781 gtaagaattc ttaataggtt taatgttggt ggcccaacat ataacgttgc atatcttaca
      841 aaatatattt catctgaata ttccacaatt ttacttggag ggcaaaaaaa cgaaagtgaa
      901 gcaagctctg aatatatttt acaagaactt gacgttgcat atactataat tcccgaaatg
      961 aaacgaagta ttggtatatg gaatgaaata aaagctctac gaaaacttat tcgcataatt
     1021 aaaacagaaa aacccgatat agttcacaca catgcggcaa aagcaggaac gctgggaaga
     1081 atagcagctt ggtattgcgg tgtaccatac atttttcata cattccacgg tcatgttttt
     1141 cattcatatt ttaacccttt gactaccaaa atatttataa caatagaaca acttttggca
     1201 aaaacatgta ccgcaattgt agctataagt gatattcaaa aacatgaaat cggtactgtt
     1261 ttcaaaattt gtcctcctca taaaattgaa gtaattccat taggttttga tttagaaaaa
     1321 ttcacaaaag atattcaaac caaacgttta caattcagac aacaatacaa tttagaacac
     1381 aatgaaattg caattggaat tgttggaaga ttagttcccg taaaaaatca tgaacttttt
     1441 atacaagcat ttcaaaaatg tttacacata agcacaaaac ctattaaagc atttataata
     1501 ggtgatggtg aaatgcgtca tgaattagaa tctttatgga atacgataga acccggtaaa
     1561 aataattcaa aacttacgtt tacatcgtgg atacaagatg ttgatgttgc atatgcagga
     1621 cttgatatag tatgcttaac atcgcataat gaaggtactc cggtaagttt aattgaagca
     1681 caagcagcag gcaaacctat agtttcaacc aatgttgggg gtattcaaaa tatagttatt
     1741 gaaggaaaaa ccgcactact tacaaacaat actgttgaag atttttcaaa caaattactt
     1801 caaattattg aaaatgaaga gttacaaaaa caaatgtcta atcattcaca acagttttct
     1861 ttagaaaaat tcggatacaa acgattgtgt aaagacatgg aacatatgta tgaaaaatat
     1921 attacatgag atttcattcg caattacata ctataaaata aattcgtact tttgtattat
     1981 agtaattaat taaaacctca ttgttgaatg aaattacaag cattagattt tttaataata
     2041 cttctttata ttgcagctac agtatttata ggattgtact ttaaaaaacg agccgaaaaa
     2101 aacaaatcgg aatatttatt aggcggcaat accatgcgtt ggtatatgct gggagtttct
     2161 gacgcttcgg gtatgtttga tatttcgggt actatgtggt tggttacact tatgtttgta
     2221 tatgggttaa aaagtatatg gattccatgg ttatggccta catttaatca aatattttta
     2281 atgatgttta ttt
//
LOCUS       DAOTOW010000365         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36979_length_2276_cov_3.250788, whole genome shotgun sequence.
ACCESSION   DAOTOW010000365 DAOTOW010000000
VERSION     DAOTOW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36979_length_2276_cov_3.250788"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="P5243_07015"
     CDS             complement(<1..572)
                     /locus_tag="P5243_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS19236.1"
                     /translation="MAYKILIADDEPDIVEFLQYNLERNGFTVFVSTNGKDAIQMAIQ
                     EKPDIILLDIMMPEVDGIEVCSDLRDRPEFNDTIIAFLTARGEDYSQIAGFEVGADDY
                     ITKPIKPRVLIARLHALLKRKRLIETNTTMLQFGHLQIDKKKRLVILHNEEIHFPKKE
                     FEILNLLASKPEVVFTREEIYQCVWGFDIFV"
     gene            complement(798..1619)
                     /gene="panB"
                     /locus_tag="P5243_07020"
     CDS             complement(798..1619)
                     /gene="panB"
                     /locus_tag="P5243_07020"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRS19237.1"
                     /translation="MSTAGSILAKVVTTHVLSEMKARNEKISMLTAYDYSMAKILDQA
                     GIDVILVGDSASNVMAGFVTTLPITLDNMIYHASSVQRAVQRALVVVDMPFGSYQGNS
                     REALSSAIRIMKETGCSAVKLEGGEEVIESVQRIISAGIPVMGHLGLMPQSINKFGTY
                     AVRAQSQEEADKLMHDAKLLQKAGCFALVLEKIPAELAAKVANSLSIPVIGIGAGSGV
                     DGQVLVLHDMLGINQEFSPRFLRRYHNLFQEITNSVQSYIRDVKTRDFPNKNEQY"
     gene            1891..>2276
                     /locus_tag="P5243_07025"
     CDS             1891..>2276
                     /locus_tag="P5243_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRS19238.1"
                     /translation="MKYGYFDDANREYVITNPQTPYPWINYLGNEDFFSLTSNTGGGY
                     TFYKDAKFRRLTRYRYNNVPIDNGGKYFYIKDGDTIWSPGWKPVKTELDSYECRHGMS
                     YSKIKGSKNGIETDVLQFIPLGFWGE"
BASE COUNT          792 a          382 c          329 g          773 t
ORIGIN      
        1 acaaaaatat caaaccccca aacacattga taaatttctt cacgagtaaa taccacttca
       61 ggttttgagg caagtaaatt aagaatttcg aattcttttt ttggaaaatg aatttcttca
      121 ttatgtaaaa taaccaaacg ctttttctta tctatttgca aatgaccaaa ttgaagcatt
      181 gttgtatttg tttcaattaa gcgttttcgc tttaataaag catgtaatcg agcaataaga
      241 acacgtggtt taataggctt tgtaatataa tcgtcggcac caacttcaaa accggcaatt
      301 tgagaataat cttcgcctcg tgctgttaaa aaagcaataa ttgtatcatt aaattcgggt
      361 ctgtctcgta agtcgctgca tacttcaata ccgtcaactt caggcatcat tatatcgaga
      421 agaattatat cgggtttttc ttgaatagcc atttgtatgg catcttttcc atttgtagag
      481 acaaaaacgg taaatccatt acgttcaaga ttatattgta aaaattctac aatatcaggt
      541 tcatcatcag ctattaaaat tttatatgcc atactaacaa atatttttct gttcaaaaat
      601 aaaagtagta tgtaaatatg attttatttc agggttactt aattgttaag taatatgatt
      661 ttatgaaaaa atttattaca gcaggatacg aatgtaaatt aaaaattaga ttataaaccg
      721 agaagttgtt attaatatat catctcttta agattataaa aataaaatat ttctaactct
      781 taattcttaa tacaaattca atactgttca tttttattgg ggaaatcacg tgtttttaca
      841 tcgcgaatat atgattgtac cgaatttgta atttcttgaa ataaattatg atatctacgt
      901 aaaaaacgtg gagaaaattc ttgatttatc cccaacatat catgcaatac caatacttgc
      961 ccgtcaacgc cactaccggc accaatacct attacaggaa ttgaaagaga attggcaact
     1021 tttgcagcta attctgccgg aattttttcc agaactaaag caaaacaacc ggctttctgc
     1081 aacaatttag catcgtgcat aagcttatcg gcctcttctt gagattgagc acgaacagca
     1141 tacgtaccaa atttattaat agattgaggc attaatccta aatggcccat aaccggaatt
     1201 cctgcggata taatacgttg taccgattca attacttctt cgccaccttc taatttcaca
     1261 gcactgcatc cggtttcttt cattattcta atagcagaac ttaatgcttc acgagaattg
     1321 ccttgatacg aaccaaacgg catatctaca acaaccaaag ctcgttgaac agcacgctga
     1381 acagacgatg catgatatat catattatct aatgtaattg ggagagtagt aacaaatcct
     1441 gccataacat tcgatgcaga atcacctacc aatattacat ctattccggc ttgatcaaga
     1501 atttttgcca tagaataatc atacgctgtt agcattgaaa ttttttcgtt tcgtgctttc
     1561 atttcactca acacatgtgt agtaacaact tttgccaaaa tagaacctgc tgtagacata
     1621 tatttgtatt taataatttt gtaatgtaaa actacagtta tttttataaa aaatacatga
     1681 acatttaaaa atattatata ctcatctttt tttgtcaata caaacaatta taaatctatc
     1741 tgattttgag tatttacact caatcagcaa tttacctgtt tttttgttaa aaaagtatag
     1801 gtttgtatgc tgcaagtttt aataaaaaaa tactatattt cgccactttt tacaatttga
     1861 taaaactaaa ttattttaac tttttactct atgaaatacg gatattttga tgatgcaaac
     1921 agggaatatg tgattacaaa tccacaaacc ccatatccat ggattaacta tttaggaaac
     1981 gaagattttt tttcgttgac ttcaaatacc ggaggtggtt atacttttta taaagacgct
     2041 aaatttcgtc gtttaactcg atatagatat aacaatgtgc caatagataa tggcggtaaa
     2101 tatttttata ttaaagatgg cgataccata tggtcaccag gatggaaacc tgtaaaaact
     2161 gaacttgaca gctatgaatg tcgtcatggt atgagctatt caaaaattaa aggttcaaaa
     2221 aatggaatag aaacagacgt tcttcaattt attccacttg gtttttgggg agaaat
//
LOCUS       DAOTOW010000366         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_36997_length_2275_cov_3.297748, whole genome shotgun sequence.
ACCESSION   DAOTOW010000366 DAOTOW010000000
VERSION     DAOTOW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36997_length_2275_cov_3.297748"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="P5243_07030"
     CDS             <1..1454
                     /locus_tag="P5243_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19239.1"
                     /translation="TYIKEFSKTRTIYPVANDDLWNEFNQILELANVGDSCKVFLKNN
                     GIYVSNWISITPENIQKLIQILKANDDTYKANLKQLLLAAKTDIELDGVIPFLITNNK
                     QMMYKPSSEIQKEARSIAFELNVSKSIYVWDITLLNTKSVAYYFNNSFVSELTYLFNS
                     SQRNYYYSLYSLSDLHKNFSLIRTNELSKVLLFSVTSRSNTGIVFDKYISNTDYMLPD
                     TVYTELGKITEGTKFYTDISFLYKGKRYTASYEFDISDTQNTLLENAWAVKMVDKLNS
                     LYINEAYEEAEKISTNKGILTQNTAMLAIEPGAEITPCYTCPDWNNTRLMLEDRMVVS
                     NNIALATAYMPFEWYNQGYLTESYDAGFNDGITYIEQSIEKNNTELYDNGYAAGYADG
                     IFNCNMGVKEKSAIEAYPTEFTTELQVIVYSNKPQIVEVYTANGIFIESFTITKVAKL
                     QHVNSVLFSMPRGTYVLKTTINGEVSYAKVFKK"
     gene            complement(1900..>2275)
                     /locus_tag="P5243_07035"
     CDS             complement(1900..>2275)
                     /locus_tag="P5243_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS19240.1"
                     /translation="LYKYIYGILRNLGQYPLAINGYKDHVHIFFELQPTKCLSDIMRD
                     IKTNSSKWINENNYVKGRFSWQQGYGGFSYSKTQRDSVIKYIINQEKHHQKNTFEEEY
                     MDLLSKFNIEFKEEYIFDFYDL"
BASE COUNT          848 a          309 c          363 g          755 t
ORIGIN      
        1 atacttatat aaaagaattt agtaaaacaa gaactatata tcctgttgca aatgatgatt
       61 tatggaatga atttaatcaa atattagaat tagcaaatgt cggcgattcg tgcaaagtgt
      121 ttttgaaaaa taatggtata tatgtgagta attggatatc cataacaccg gaaaatattc
      181 aaaaactcat acaaatactc aaagcaaacg atgatactta taaggcaaat ctaaaacaat
      241 tgttgttagc cgcaaaaacg gatatagaac ttgatggggt aatacctttt ttaataacaa
      301 acaataaaca aatgatgtat aaaccaagta gtgaaataca aaaagaagca cgaagtatag
      361 cgtttgaact caatgtgtca aaatcaatat acgtatggga tattacatta ctcaatacaa
      421 aatcagttgc atattatttt aataactctt ttgtatcaga gttaacctac ttatttaatt
      481 cttcacagcg aaattattat tattcgttat actcattatc tgatttgcat aaaaattttt
      541 cattaataag aacaaatgaa ttgtcaaaag tactgttatt ttcagtaact agtcgtagta
      601 atacaggaat agtatttgat aaatatatat caaatacaga ttatatgttg ccggatacag
      661 tatatacaga acttgggaaa attacagagg gaacaaaatt ttataccgat ataagttttt
      721 tgtataaagg gaaacgctat accgcttcat acgaatttga tatttctgac acacaaaata
      781 cattattaga aaatgcttgg gctgttaaaa tggttgataa actaaatagt ttgtatataa
      841 atgaagcgta tgaagaggca gaaaaaataa gtactaacaa aggtattctt acacaaaata
      901 ccgctatgtt ggcaatagag cccggagccg aaattacacc atgttacact tgtcctgatt
      961 ggaataatac acgtttgatg ttagaagaca gaatggttgt ttccaataat atagcacttg
     1021 ctaccgctta tatgcctttt gagtggtaca atcaagggta tcttacagaa agttatgatg
     1081 caggattcaa tgatggaatt acatatattg aacaaagtat tgaaaagaat aatacagaat
     1141 tgtatgacaa tggctatgca gcaggctatg cagacggaat atttaattgt aacatggggg
     1201 ttaaagaaaa atctgcaatt gaagcatatc ctaccgaatt tactaccgaa ttacaggtta
     1261 tagtgtatag caataaacca caaatagtag aagtatatac agcaaacgga atatttatag
     1321 aatcatttac aattacaaaa gttgcaaaat tacaacatgt aaattctgta ttgttttcaa
     1381 tgccaagagg cacgtatgtg ctcaaaacta ctataaatgg agaggtttcg tatgctaagg
     1441 tgtttaagaa ataaattgaa tgttagtaat ctacaatagt agaaatttat ttggctttat
     1501 agctcataat ttattgcata gttaaaaaat tgtcaaaaaa gacagccatt tatctactat
     1561 ctatcattac ctctccgagg catgaatggt taatactatt atgtattcaa ttagctcaat
     1621 aggtgcggta gtatggtagg aaatatatat aaatattgag cattatagct tccaaggagc
     1681 gacagtaaat atatattaca gaatatatca cctcgctgag gcttatttta aatgcatgta
     1741 ttttattacc agagtgtcgc ctctccgagg catgaatggg taaaaccatt atgtattcaa
     1801 ttagctccgt aggagcgtta gtatggtagc acagataatg tttgtgggat aaagctccgt
     1861 aggagcgtta ttattgtaaa ttatctgtaa ttaaaaaaat tacaaatcgt aaaaatcaaa
     1921 aatatattct tctttaaatt caatattaaa tttgcttaat aaatccatat attcttcttc
     1981 aaaagtattt ttttggtgat gcttttcttg attaataata tattttatta cgctatctct
     2041 ttgtgttttt gaatacgaaa aacctccata tccttgttgc catgagaatc ttcctttaac
     2101 atagttattt tcattaatcc atttagatga atttgtttta atatctcgca taatatccga
     2161 gagacacttt gttggttgta attcaaaaaa gatatgaaca tgatctttgt atccatttat
     2221 agctaatgga tattgtccta aatttctcaa aattccatat atgtatttat ataat
//
LOCUS       DAOTOW010000367         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37120_length_2269_cov_3.391599, whole genome shotgun sequence.
ACCESSION   DAOTOW010000367 DAOTOW010000000
VERSION     DAOTOW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37120_length_2269_cov_3.391599"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="nqrA"
                     /locus_tag="P5243_07040"
     CDS             complement(<1..1060)
                     /gene="nqrA"
                     /locus_tag="P5243_07040"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HRS19241.1"
                     /translation="MSKVYKLRKGLTIPLKGEAERIVHQLPLPKTVAIKPTDFIGVVP
                     KLVVKEGDTVLAGDVLFIDKNNQSIQFTSPVSGVVQTILRGERRKLLEVVVAVDSEIS
                     YKKFEIPTDITNREAIIDFMLQSGVWPFVKQRPFNCIAKPTDIPKAIHVSAFDTAPLA
                     PDYPFVISEYKELFQKGLQILNALTPGKLHLNILEENRTLHPFDECCGDVQKNYFSGI
                     HPAGNVGVQIHHIDPINKGDVVWTLNPQDVVIIARLFEKGIFDAHKTIAVTGNLQKPH
                     YVKTILGAAVNDCFEEQIVENQRIISGNVLTGTNVGYKGYIGFYDSQITVIPEGNKPE
                     FMGWIAPGFEKFSLSRTFF"
     gene            complement(1256..>2269)
                     /locus_tag="P5243_07045"
     CDS             complement(1256..>2269)
                     /locus_tag="P5243_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS19242.1"
                     /translation="KKSVDYDAFLVTSEHKKIQVSLSCNPIFLDNKNDFGIVFIGRDS
                     TERKKAEARLRNENIKLEDLVSKRTENLSKINTQLLVEISKYEKTEKKLLQTIQQLEV
                     NERNKIAFFDNLAHEISTPVNAIIGFSDLLRDDQITKEDIIKYTSIIQASSLQLQGIV
                     KNVVSMATINNNKEVVNLEEVNCNYILNGLHTLFKPKADKKKLEFTCFKKLRDEEAIV
                     ITDQIKLTQIISNLLTNAFKFTSEGYIHFGYTISTDKITFFVRDTGIGIPEESREKIF
                     ERYAQGNPTISELFGGSGLGLSIAKTYVELLGGQIWIEPNTTKGSNFNFWLPYSYQIE
                     KIH"
BASE COUNT          725 a          385 c          303 g          856 t
ORIGIN      
        1 aaaaaaatgt tctgctcaaa ctgaatttct caaatccggg agctatccac cccataaatt
       61 caggtttatt tccttcgggt attacggtaa tttgcgaatc ataaaaccct atataccctt
      121 tgtaaccaac atttgttcct gtaagcacat ttccacttat aattctttga ttttcgacaa
      181 tttgttcttc aaaacaatca tttaccgccg ctcctaaaat agttttcaca tagtgcggtt
      241 tttgaagatt tccggtaaca gctattgttt tgtgcgcatc aaatattcct ttttcaaaca
      301 aacgagcaat aattacaaca tcttgaggat tcaaagtcca tactacatct cctttattta
      361 taggatctat atgatgaatt tgcactccta cattacctgc aggatgaatt cccgaaaaat
      421 aatttttttg aacgtcgcca cagcactcat caaaaggatg cagcgttcta ttttcttcta
      481 aaatattaag atgcagtttc cccggggtta aagcattgag tatttgcaag cccttttgaa
      541 ataattcttt atactctgat attacaaatg gataatcggg agcaagggga gctgtatcaa
      601 atgcagatac atgaattgct tttggaatat cagttggctt tgcaatacaa ttaaacggac
      661 gttgttttac aaaaggccac acaccacttt gcaacataaa gtctattatt gcttctctgt
      721 tggttatatc agtaggaatt tcaaattttt tgtacgaaat ttctgaatct acggcaacta
      781 caacttcgag taacttacga cgttcgcctc gtaatatagt ttgcaccaca ccactcacag
      841 gtgatgtaaa ttgtattgat tggttatttt tatcaataaa caaaacatca ccggccaata
      901 cagtatcacc ttcttttaca actaactttg gaactactcc tataaaatct gttggtttta
      961 ttgcaactgt tttggggagt ggtaattgat gtacaattcg ttctgcttca cctttcagtg
     1021 gaattgttaa acctttacgt aatttatata cttttgacat atgctgacat ataattcagt
     1081 tattgctttc aaaagtacac atttttaaat aattataaaa aatgaagtca agaatttttt
     1141 atttaaaaaa aacttacttt tatatgatac aaaaccatgt ttttaatact acatatatgc
     1201 tattagattg taatataaaa actaaaaaaa agactaatat gattttattt tttaattaat
     1261 gtattttttc aatttgatag gaatatggca accaaaaatt aaaattactg ccttttgtag
     1321 tattgggttc aatccaaatt tggccgccta acaattcaac atatgttttt gctattgaca
     1381 atcctaatcc tgaaccacca aataattctg aaattgttgg attaccttga gcatatcgtt
     1441 caaatatttt ttctctgctt tcttcaggta ttcctatacc ggtatcacgc acaaagaatg
     1501 ttattttatc ggtagaaatt gtatatccaa aatgaatata gccttcgctg gtaaacttaa
     1561 atgcattggt taataaatta gaaataattt gcgtaagttt tatttgatct gttataacaa
     1621 ttgcttcctc gtcacgtaat tttttgaaac aagtaaattc aagttttttc ttatctgctt
     1681 taggtttaaa caatgtatgt aatccattga gtatataatt acaattaacc tcttctaaat
     1741 ttacaacttc tttgttatta ttgatggtag ccatacttac cacatttttg actatacctt
     1801 gtaattgtaa agaactagct tgaattatag aagtatattt aataatatct tcttttgtta
     1861 tttgatcgtc tcgtaacaaa tcactaaacc caataatagc atttacggga gtacttattt
     1921 catgtgctaa attatcaaaa aaagctatct tatttcgttc atttacttca agttgttgaa
     1981 tagtttgcaa taattttttt tcggtttttt catatttact aatttcaacc agcaattgag
     2041 tatttatttt tgataaattt tctgttcgtt ttgaaactaa atcttcaagt ttaatatttt
     2101 cgtttcgtaa ccgtgcttct gcttttttac gttcggtact atcacgacct ataaacacta
     2161 ttccaaaatc atttttatta tctaaaaaaa ttggattaca cgacaatgaa acttgaattt
     2221 ttttatgttc tgatgttact aaaaaagcat cataatcaac cgatttttt
//
LOCUS       DAOTOW010000368         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37122_length_2269_cov_3.226287, whole genome shotgun sequence.
ACCESSION   DAOTOW010000368 DAOTOW010000000
VERSION     DAOTOW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37122_length_2269_cov_3.226287"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="P5243_07050"
     CDS             <1..971
                     /locus_tag="P5243_07050"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRS19243.1"
                     /translation="ANVWCIYQNGTWGKIEIHTEETVTLHMAATCLHYGQECFEGLKA
                     YRGVDNSIRLFRWEDNAKRMSKSAQRIGMPEIPMNIFKEALFTLIAHNKEFIPPYGTG
                     ATMYIRPLMIGTEGKLGLNASTEYMFIMFCSPVGPYFKDGCKPVKVIIERDSDRAAPL
                     GTGNVKVGGNYAAAVNATLRAHQLGYSSALYLDPKEKKYIDECGPANFFAIKNNTYVT
                     PQSTSILESITNMSLITLAANMGMKVEKRQIPLDELGSFEEAGQCGTAAVITPICEIY
                     DPLLHISHTYGSPNTIGEKSKLLYDTLVGIQFGEKEDTEQWITIIH"
     gene            987..1655
                     /locus_tag="P5243_07055"
     CDS             987..1655
                     /locus_tag="P5243_07055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRS19244.1"
                     /translation="MFVDVLYEDNHVIIVNKATNVLVQSDTTGDTSLEDAVKLYIKEK
                     YNKPGEVFLGVIHRIDRPVSGVVLFAKTSKALTRLNEQLKKKEFKKTYRAIVKQKPIP
                     ESSILKHYIVRNPKINKSIAYDKPTADAKEASLAYRTIAQSQNYYLLDIDLHTGRHHQ
                     IRCQLAKIGCPIKGDLKYGFARSNQNGGISLHAYSLTFVHPVSKEEISVIAPLPHDDI
                     WHVF"
     gene            1728..>2269
                     /locus_tag="P5243_07060"
     CDS             1728..>2269
                     /locus_tag="P5243_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19245.1"
                     /translation="MIQYTSAYDFIVSLLAIGIIFVIVSNKQQTYESRYEYFSVLTKA
                     TFIRIGAGLFFSYIYLIYYGDGDTVFYYSGAGTIVNMAKKDVFAFIQLMFGERTPELR
                     SLFDWTTGYPTYFKDANAWAVCRFTVPFYIAGLGSYWASTIIMNAVLMIPMWRFYTML
                     IRMYPQQRKYMAIALFYMPS"
BASE COUNT          772 a          340 c          421 g          736 t
ORIGIN      
        1 atgctaatgt ttggtgtata taccaaaatg gtacgtgggg gaaaattgaa attcatacag
       61 aagaaacagt tacactacat atggcggcta cgtgtttgca ctatggtcaa gaatgttttg
      121 aaggtttaaa agcatataga ggagttgata attctattcg cttattccgt tgggaagata
      181 atgcaaaacg tatgagtaaa tctgcacaac gtattggaat gcccgaaata ccaatgaata
      241 tattcaaaga agcattgttt acattaatag cacataataa agaatttatt ccgccatacg
      301 gaacgggagc aacaatgtat attcgccctc ttatgatagg aaccgaggga aaattaggtt
      361 taaatgcaag taccgaatat atgtttatta tgttttgtag tccggtaggt ccttatttta
      421 aagatggatg caaaccggta aaagttataa ttgaacgtga tagtgaccgc gcggctcccc
      481 ttggaacagg gaatgtaaaa gtgggcggaa attacgctgc agctgttaat gcaacactac
      541 gtgcacatca attagggtat agttctgcat tatatcttga tcctaaagaa aaaaaatata
      601 tagatgaatg tggtcctgcc aatttttttg caattaaaaa taatacgtat gttacaccgc
      661 aatctacatc tattttggaa tcaattacca atatgagttt aattacctta gcagctaata
      721 tgggaatgaa agtagaaaaa agacagattc cattagacga acttggttct tttgaagaag
      781 caggacagtg tggtacggct gcggttatta cacctatttg tgaaatttat gatcctcttt
      841 tacatatttc ccatacgtat ggatctccta atactatagg tgaaaaaagc aagttattgt
      901 atgacactct tgttggtatt caattcggag aaaaagagga taccgaacaa tggataacaa
      961 ttattcacta attcctattc ttacgtatgt ttgttgatgt gttatatgaa gataatcatg
     1021 taataatagt taacaaggct acgaatgttt tggtgcaatc tgatactacc ggtgatacat
     1081 cattagaaga tgccgtaaaa ctatatatta aagaaaaata taataaaccg ggtgaggtgt
     1141 ttttgggtgt gattcatcgt atagacagac cagtgagcgg agttgtgttg tttgccaaaa
     1201 caagtaaagc tcttactcga ttaaatgagc aactcaagaa aaaagaattt aaaaaaacat
     1261 atagagctat tgtaaaacaa aaacccattc ccgaatcatc aattttgaaa cattatattg
     1321 taagaaaccc aaaaataaat aaatcaattg cttatgataa acctactgcc gatgcaaaag
     1381 aggcaagttt agcatacaga acaattgctc aatctcaaaa ttattatttg ttagacatag
     1441 atttgcatac cggtagacat catcaaatta gatgtcaact tgctaaaata ggatgtccta
     1501 ttaagggaga tttaaaatat gggtttgctc gtagcaatca aaatggaggt atttctttac
     1561 atgcatattc acttacattt gttcatcctg taagcaaaga agaaatatcg gtaattgcac
     1621 ctctgcctca tgatgatata tggcatgttt tttaaataag taacatgtca cttaatcaat
     1681 tatttaaaaa cacaatgcaa actcatatat tttcgtaatt ttgaagtatg attcaatata
     1741 cctcggcata tgattttata gtttccttat tagcaattgg gattatattt gtaattgtta
     1801 gtaataagca acaaacatat gaatcaagat acgaatattt tagtgtatta acaaaagcta
     1861 catttattcg gataggtgcc ggattatttt tttcatatat ctatttaatt tattatggtg
     1921 acggcgatac tgttttttat tattccggag ccggcactat agtaaatatg gcaaaaaaag
     1981 atgtgtttgc ttttattcaa cttatgtttg gtgagcgcac acccgaacta cgttcattat
     2041 ttgattggac taccggttat cccacgtatt ttaaagatgc taatgcatgg gctgtatgta
     2101 gatttacggt tcctttttat atagccggtt tggggtcata ctgggcaagt actattataa
     2161 tgaatgctgt tcttatgata cctatgtggc gattttatac aatgcttata cgtatgtatc
     2221 cgcagcaaag aaaatatatg gctatagcat tattttatat gccttcatt
//
LOCUS       DAOTOW010000369         2261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37285_length_2261_cov_2.895286, whole genome shotgun sequence.
ACCESSION   DAOTOW010000369 DAOTOW010000000
VERSION     DAOTOW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37285_length_2261_cov_2.895286"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..401)
                     /locus_tag="P5243_07065"
     CDS             complement(129..401)
                     /locus_tag="P5243_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRS19246.1"
                     /translation="MNKQELVAAIAAESGLTKVDSEKALNATIKAISAGLKKGESIQL
                     VGFGTFSVGARAARTGRNPQTGKAIKIAAKKVAKFKAGKALSDAVN"
     gene            complement(591..833)
                     /locus_tag="P5243_07070"
     CDS             complement(591..833)
                     /locus_tag="P5243_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HRS19247.1"
                     /translation="MEHELPEYTERQLSLKNGQDSPEIWVAYKGLIYDMTHSLKWYRG
                     MHYHHWSGQDLTDEIDKAPHSEQVFLKFLPIGKLKK"
     gene            complement(854..2188)
                     /locus_tag="P5243_07075"
     CDS             complement(854..2188)
                     /locus_tag="P5243_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRS19248.1"
                     /translation="MAKKKNKIFVLDTNVILHNYRAIYAFRENDIVIPITVLEELDKF
                     KKGDNELSWSARQFTRELDAIAGDKLFTDGVPLGEGLGKLYVKTHAEYPAIVKNAFAE
                     QIPDHRILATAIIVKEKNKEIPVILVTKDINLRMKAKSIGLTAEDFENDKLQDLDTIE
                     EGVRVINNVDSALISKMYGKTGDLSITPEEIGIEHPTSHEYFILKSDKSSIIAYYNAN
                     EKVITRVVKRQVFGIEPRNAEQTFALHALNNKDIRLIAITGKAGTGKTLVALAAALEQ
                     HEHYEQILLARPIVALGNKDLGYLPGGEKDKIRPYMLPLFDNIQVIKQNLHRNGEQVN
                     LIDNMLKDGRLEISPLAYIRGRSLPKVFFIIDEAQNLTPHEVKTIITRAGEGTKIIFT
                     GDIQQIDSPYLDSFSNGLSVLSTKMKGQKIFTHINLVRGERSELAELASTLL"
BASE COUNT          687 a          396 c          267 g          911 t
ORIGIN      
        1 aataaaaaaa agtaataaat aataataatt tttactacaa atatttctac acatttgtag
       61 aatttaataa aactgcaaaa aaaaagctgt tcaagtatga acagcttttt ttatgataag
      121 ttgtactatt agttaactgc atcagataaa gcttttccag ctttgaattt agcaactttt
      181 tttgcagcaa ttttgatagc ttttccagtt tgtgggtttc ttcctgtacg agcagcacga
      241 gcacctactg agaaagttcc gaaaccaact aattgaatac tttcaccttt ttttaatcct
      301 gcagagattg ctttaattgt tgcatttaat gctttttctg aatctacttt agttaaccca
      361 gattctgctg caattgcagc tactagttct tgtttgttca tagtaagtta atttttagtg
      421 agtactattt ttaatataat tatttctatt tttacttctt tttttttcta aaaattctct
      481 aaattctttg ccaattgagg atttacaata caattataca aaaaaaaaat tacataacac
      541 ctttcaacac gtattttttt tatttttttt agtttttttt ctgaaatttc tcattttttt
      601 aactttccta taggtaaaaa cttcaaaaat acttgttctg aatgcggtgc tttatctatt
      661 tcatctgtta aatcctgacc actccaatga tgataatgca tccccctata ccactttaaa
      721 ctatgtgtca tatcatatat caatccttta tatgcaaccc atatttcggg tgaatcttgc
      781 ccatttttca atgacaattg acgttctgta tattcaggta attcatgttc catattttta
      841 aataactctt accttataat aaggtacttg ctaattcagc caattcacta cgttcacctc
      901 taactaaatt tatatgagta aatatttttt gtcctttcat tttagtactt aataccgata
      961 aaccatttga aaaactatcc aaatacggtg aatctatttg ttgaatatcg ccggtaaaaa
     1021 taattttggt tccctctcct gctctggtaa taattgtttt cacctcatgg ggagttaaat
     1081 tttgtgcttc atctattata aaaaacactt taggcaatga acgtccacgt atatatgcta
     1141 acggagatat ttccaaacgt ccgtctttaa gcatattatc tattaaattt acttgctcac
     1201 cgtttctgtg taaattttgt tttataactt gtatattatc aaacaatggt aacatgtacg
     1261 gacgaatctt atctttttca cctcccggca aatatcctaa atctttattt cctaaagcta
     1321 caataggacg agcaagtaat atttgctcat aatgttcatg ttgctcaagc gctgcagcca
     1381 atgcaactaa tgtttttccc gttcctgctt ttccagtaat tgcaattaat cgtatatctt
     1441 tattattaag tgcatgtaat gcaaatgttt gttctgcatt tcgtggttca ataccaaata
     1501 cttgacgttt aactacacgt gttataactt tttcgttggc attataatat gcaattatac
     1561 tggatttatc actttttaat ataaaatatt catgacttgt aggatgttct atacctattt
     1621 cttcaggagt tatacttaaa tcccccgttt ttccatacat tttacttatc aatgcagaat
     1681 ctacattatt tatgactcgt acaccttctt ctatagtatc taaatcctgt aatttatcat
     1741 tttcaaaatc ttcggctgtt aaaccaatag attttgcttt catacgtaga tttatatctt
     1801 tggtaactaa tataaccggt atttctttat tcttttcttt tactatgata gctgttgcta
     1861 atattctatg atcgggaatt tgttctgcaa aagcattttt cactattgcc ggatattccg
     1921 catgagtttt tacatataat tttcctaaac cttcacctaa aggaactcca tcggtaaaaa
     1981 gtttatctcc agctatagca tctaattcac gtgtaaattg tcgtgcactc caacttaatt
     2041 cattatcacc ttttttaaat ttatctaatt cttctaaaac ggtaattggt attactatat
     2101 cgttttctct gaacgcataa attgctcggt aattatgtaa tattacgttt gtatctaata
     2161 caaaaatttt atttttcttt tttgccataa tttgttttta atttacaaaa tcattattgt
     2221 ccgataaaac tacaaaatta atttaataaa acaaaattta t
//
LOCUS       DAOTOW010000370         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37571_length_2249_cov_2.321331, whole genome shotgun sequence.
ACCESSION   DAOTOW010000370 DAOTOW010000000
VERSION     DAOTOW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37571_length_2249_cov_2.321331"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="P5243_07080"
     CDS             <1..886
                     /locus_tag="P5243_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRS19249.1"
                     /translation="EEFFDQLYMIRNIIISIISLLLVAIFIVMRMSAREILSADQAFW
                     DTMMKNLNKVLVPVIEIMGIEKVAGEDNVSSLLHSEAIVGFMSGKIGPVLIAIRQTVV
                     MLLMAVFFLLLLMAGSINVQKVMENTIFKKRIPSMKTFVILEKSIVKFIVVKFLISLA
                     TGISFSIACVSFGIPFPVFWGTMAFLLNFIQMVGSIVITIVLSVFGIALLPPGLGVVA
                     FIFILIAIQILFGSVLEPIFMGKTFSINTITILIMILLWGYIWGVPGLIFSVPITVAL
                     KTILEQFPKTRIIAQIMS"
     gene            897..>2249
                     /locus_tag="P5243_07085"
     CDS             897..>2249
                     /locus_tag="P5243_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRS19250.1"
                     /translation="MNSTIISRIAHNVGVSHTSVQNTFNLFQTGATIPFIARYRKEQT
                     GSLDEVQLLAIKEQIDYYESLEKRKVTILTTIEEQGNLTSELQKTIEQCYNAIELEDI
                     YLPFKPKKKTRASEAKLKGLEPLANILFEQKHIQFSNVVSEFAKNIHLHEADALQGAR
                     DIIAEWISEHSEVRKQLRAIFVKQSVISSKVVKTKESEAEKYNQYFNFQELACKSPSH
                     RVLAMMRGENEGFLRLSISIDEEIAYAIIYKRVLKAYNECAQHVKMAAEDAYKRLIAP
                     SLEKEMYNDLKERADAEAITVFSQNLRQLLMLPPLGQVRILAIDPGYRTGCKVVCLNE
                     QGDLLHNETIYPHAPHNDTAKAMNKIAQLVQTYNIQAISIGNGTAGRETEQLISRIRF
                     NTDVQVFVVNEAGASVYSASKVAREEFPQYDVTVRGAVSIGRRLMDPLAELVKIDPKS
                     I"
BASE COUNT          787 a          301 c          416 g          745 t
ORIGIN      
        1 ggaagaattt ttcgaccagt tatatatgat tcgaaatatt attatttcaa ttatttcatt
       61 gttgttagtt gcaatattta ttgtaatgcg tatgagtgca cgtgaaattc taagtgcaga
      121 tcaagcattt tgggatacta tgatgaagaa tttaaataaa gtattggtac ctgtaattga
      181 aataatggga atagaaaaag ttgccggtga agataatgtg agttctttgt tgcatagtga
      241 agctattgta ggttttatgt caggaaaaat tggacccgta ttaatagcaa tcagacaaac
      301 agtggtaatg cttttgatgg cagtgttttt tttgttgctt cttatggccg gatctattaa
      361 tgttcaaaaa gtaatggaaa atacgatttt taaaaagcgt attccatcta tgaaaacctt
      421 tgtgatactt gaaaaaagta ttgtgaaatt tatagtcgta aaatttctta taagtttagc
      481 aaccggaatt tcatttagta tagcgtgtgt gtcatttgga attccttttc ctgttttttg
      541 gggaactatg gcatttcttt taaattttat acaaatggtt ggttctattg ttattacaat
      601 tgtgttgtct gtatttggta ttgcattgtt acctccggga ttaggggttg tggcatttat
      661 ttttatttta attgcaatac aaattttgtt tggaagtgtg cttgaaccta tttttatggg
      721 gaaaacattt tctatcaata caattacaat tcttataatg attcttttat gggggtatat
      781 atggggtgtt ccgggattaa tattttcggt tccaataact gttgcactta aaacaatttt
      841 agagcaattt cctaaaacaa gaattatagc acaaatcatg tcataaactt gtattgatga
      901 attctacaat tatatcaaga atagcacata atgttggagt ttctcataca agtgtgcaaa
      961 atactttcaa tttatttcaa acaggagcta caattccatt tatagctcgt tatcgtaaag
     1021 aacaaaccgg cagcttagat gaagtgcaac tccttgcaat taaagaacaa attgattatt
     1081 atgaatcact cgaaaaacgc aaagttacta tacttacaac aatagaagaa caaggcaatt
     1141 taacatcaga attacaaaaa acaatagaac aatgctataa tgcaattgaa cttgaagata
     1201 tttatttgcc atttaaacct aaaaagaaaa ctcgtgcttc agaggcaaaa ttaaaagggt
     1261 tagaaccttt agcaaatata ttatttgagc aaaaacatat tcaattttca aatgtagtga
     1321 gtgaatttgc caaaaatata catttacatg aagctgatgc attgcaaggt gcacgagata
     1381 taattgcaga gtggataagt gaacattcag aggttagaaa acaattacga gcaatatttg
     1441 taaaacaatc ggtaatttct tcaaaggttg taaaaactaa agaatcagaa gcagaaaaat
     1501 ataatcaata ttttaatttt caagaattgg catgtaaatc tccttctcat agagtattgg
     1561 ctatgatgcg tggtgaaaat gaaggatttt tacggttatc aatttcaata gacgaagaga
     1621 ttgcttatgc gataatttat aaacgggtgt tgaaagcata taatgaatgt gctcaacacg
     1681 tgaaaatggc agcagaagat gcatataaac gattgattgc tccttcgctc gaaaaagaaa
     1741 tgtataatga tttaaaagaa cgtgcagatg ctgaagcaat aactgtattt tctcaaaatt
     1801 tacgtcagtt gctcatgcta ccaccgttag ggcaagtaag aatacttgct atagacccag
     1861 gatatagaac cggttgtaag gtagtgtgtt taaatgaaca aggtgattta cttcataatg
     1921 aaactatata tcctcatgct ccgcataatg ataccgccaa agcaatgaac aaaattgcac
     1981 aattggttca aacatataat attcaagcaa tttctatagg taatggtact gcaggtagag
     2041 aaacagaaca attaatttcg cgcatacgat ttaatactga tgtacaagtt tttgttgtaa
     2101 atgaagcagg agcttctgta tattctgctt caaaagtagc aagagaagaa tttccgcagt
     2161 atgatgttac tgtgcgtggt gcagtttcta ttggtagaag attaatggac cctttagcag
     2221 aattagttaa aattgaccca aaatcaata
//
LOCUS       DAOTOW010000371         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37634_length_2246_cov_3.323597, whole genome shotgun sequence.
ACCESSION   DAOTOW010000371 DAOTOW010000000
VERSION     DAOTOW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37634_length_2246_cov_3.323597"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="P5243_07090"
     CDS             <1..372
                     /locus_tag="P5243_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS19251.1"
                     /translation="RAGAKGFLAHDSQRQDLIEAIYTLRNGHDYFSKCITQILLHRYV
                     QSIQNNESSSIKGIDLLSSREIEILTLWGNSYTNQEIADALFISVRTVESHKTHIMQK
                     LQLKTAVDLMKYAIKNNIIQL"
     gene            720..1883
                     /locus_tag="P5243_07095"
     CDS             720..1883
                     /locus_tag="P5243_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRS19252.1"
                     /translation="MKKIFRIVIALISTLGGVQLYAQDPNFSQFFLKEMYYNPGFIGI
                     NPGLRGTLTDRHLWTSVPGEWGTQSIAFDYYDNKFAQGGIGGHITRNTQGENFLNTFQ
                     GGVGYAKQIRVLPDLMFQIGAGAQYVYKSIDYTKMTFVDEFDPRFGRIYETEFIPPDE
                     GSWKQSVFDYNAGLVVRFNIRKTPVKYIASNSFGIAFHHLSQPDMSWVDDGNEAPLPM
                     KVNIHAYSMVRIDRSGFYNRFFLLTPGFLFENQTPTKLWFTGSEAGAKTLSFGMNALI
                     PSKISFVSSLYTGIWMRKMYIKRMALQDDIANLQNKSFDSMIFTLGYKKNSRDKKSLY
                     QIVYSYDMTISQAGLSTGGSHEITLSFEIHNLKLPGRDARKSAVPHPNDKFFK"
     gene            complement(1937..>2246)
                     /locus_tag="P5243_07100"
     CDS             complement(1937..>2246)
                     /locus_tag="P5243_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRS19253.1"
                     /translation="HREIFQKLLNQKNNADEILITKQGYITDTSYTNIIFFDGREWFT
                     PQTYLLPGTQRAYLLEQKLIREKPIHINTIHTFSKARLINCFLNLEVGNDIPIENIYI
                     "
BASE COUNT          773 a          282 c          378 g          813 t
ORIGIN      
        1 agagccggag caaaagggtt tttagcacat gattctcaac gtcaggattt gattgaagca
       61 atatatactt tacgaaacgg gcatgattat tttagtaaat gcattacgca aattttattg
      121 catagatatg tgcaatctat tcaaaataat gaaagttcca gtattaaagg tattgattta
      181 ttgagtagta gagaaattga aattcttaca ttatggggga atagttatac aaatcaagaa
      241 attgctgatg ccttatttat tagtgtaaga actgtagaat cacataaaac tcatataatg
      301 caaaaattac aattaaaaac agctgttgat ttaatgaaat atgctataaa aaataatatt
      361 attcaactgt aattcgacgt tttgggttgt taatagttta ttaaaattga tgttttttta
      421 caaaaaaatg gtaaaaaatt aagttggtgc aaattttttt cataaaaaaa taatttaaaa
      481 aacaataaat aagtgatagt taaaattttg attttcaaaa cattattgct tttttgttaa
      541 aaacttacgt ttactaaata aaaaaaaaca gcgattttct gtactttatt gtatttgcta
      601 tgttttttta atattttttt ttgtaagttt gcatatcacg gcgataacat aatattttta
      661 ctaaccgtgt tgagagaaag aattttttaa aaaattatta cgataataca caatatacaa
      721 tgaagaagat attccgtatt gtaatagcac ttataagcac acttggaggt gtacaattgt
      781 atgctcagga tcctaacttc tcacagttct tcttaaaaga gatgtattat aatccaggat
      841 ttattggtat aaatcccgga ttgcgcggta cacttaccga tcgccactta tggacaagtg
      901 ttcccggaga gtggggaact caaagtattg cttttgatta ttatgataat aaatttgctc
      961 agggtggtat aggcgggcat ataactcgta atactcaagg agaaaatttc ttaaatacat
     1021 ttcaaggagg agtagggtat gcaaaacaaa tacgtgtatt gcccgattta atgtttcaaa
     1081 taggtgcagg tgctcaatat gtgtataaaa gtattgatta tacaaaaatg acatttgttg
     1141 atgaatttga cccacgtttt ggaagaattt atgaaacaga atttattcca ccggatgaag
     1201 gtagttggaa acaaagtgtt tttgattata atgccggttt agttgttcgt tttaatatta
     1261 gaaaaacacc ggttaagtat atagcatcaa attcatttgg tatagcattt catcacttat
     1321 cgcagcctga tatgtcgtgg gtagatgatg gaaatgaagc tcctcttcca atgaaggtaa
     1381 atatacatgc ctattctatg gttcgtatag atagaagtgg tttttacaat agatttttct
     1441 tgttaactcc gggattttta tttgaaaatc aaacacctac taaattgtgg tttacgggtt
     1501 ctgaagcagg tgctaaaacc ttatcatttg gtatgaatgc gttaattcca tcaaaaatat
     1561 cttttgtatc ttctttatat accggtattt ggatgcgtaa aatgtatatt aagcgtatgg
     1621 cattacaaga tgatattgca aatcttcaaa ataaatcatt tgattctatg atatttacac
     1681 ttgggtataa aaagaattct cgtgataaaa aatcattata tcaaattgtg tatagttatg
     1741 atatgacaat ttctcaagca ggacttagta ccggaggttc tcatgaaatt acattatcat
     1801 ttgaaattca taacttaaaa ttacctggtc gtgatgctcg taagagtgct gtaccgcatc
     1861 ctaacgataa gttttttaaa tagtagaagt caataattta atataaaaaa aggagattat
     1921 aagtaatctc ctttttttat atgtatatat tttcaattgg gatgtcattc ccaacttcaa
     1981 gattaagaaa acaatttatg agtcgtgctt tactaaatgt atgaatagta tttatatgaa
     2041 ttggtttctc tcgaattagt ttctgttcta ataaatatgc tctttgagta cccgggagta
     2101 aatatgtttg aggagtaaac cattctcttc catcaaaaaa tataatgttt gtatatgatg
     2161 tatctgttat atatccctgc ttggttataa gaatttcatc tgcattattt ttttgattca
     2221 gtaatttttg aaatatttct ctatgt
//
LOCUS       DAOTOW010000372         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37684_length_2244_cov_3.352672, whole genome shotgun sequence.
ACCESSION   DAOTOW010000372 DAOTOW010000000
VERSION     DAOTOW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37684_length_2244_cov_3.352672"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="P5243_07105"
     CDS             complement(<1..723)
                     /locus_tag="P5243_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS19254.1"
                     /translation="MYFKKLLITSFLFCAAHIMLNAQTETINTIVIEQNQEAELAYNN
                     GIQLLNAQQYGQAIQQFELAIRYNPQFLKAYINLGAAKTYTNDLDGAISAYNYVLGVD
                     STIAQVWYSRAMVYFSKNDFMSCLRDVTKAILLQYETAEAYYYQGVCMFFIKDYKGAV
                     SAYTNAIQLNPRYAYAYNDRGSARRMMGDIDRAVGDYEQALRLNPEMANTYDNLGSVL
                     LKSGDIEGAIRNFDLAIKWKPDF"
     gene            complement(911..1894)
                     /locus_tag="P5243_07110"
     CDS             complement(911..1894)
                     /locus_tag="P5243_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS19255.1"
                     /translation="MIPNFDSSIFTIDSENTFNEYAIRLFQYQSVHCTVYASFIKHLG
                     IQPHTITHYSQIPCLPISFFKSHTVVSSSLPVETIFESSKTTGTIASKHYIPSLNIYR
                     QSFMTMFKRIYGNPQEYIFLALLPSYLERSNSSLVYMIQHLIQESQNTASGFYLHNFD
                     DLHNAIQTYSHSKIFLIGVTFALLDFAEQYTCNHNNLIVMETGGMKGRKKEMIRSEVH
                     EILQKSFGVSHIHSEYGMTELLSQAYALRNGEFTPAPWMKVLIRDMYDPRYYVPNGTH
                     GGINIIDLANWYSCAFIETQDIGRILHTNTFSVEGRIDSSDIRGCNLMVQS"
     gene            2009..>2244
                     /locus_tag="P5243_07115"
     CDS             2009..>2244
                     /locus_tag="P5243_07115"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRS19256.1"
                     /translation="MKLLAHIILLIVMMNCSGNSQVILQEQKNDTIMKYRTLNEFEQY
                     VILNKGTERPFSGEYTDLFEEGVYACKQCGAELYT"
BASE COUNT          746 a          334 c          379 g          785 t
ORIGIN      
        1 gaaatcgggc ttccatttta ttgcaaggtc aaaatttctg atagcacctt caatatcacc
       61 ggattttaat agtaccgaac ctaaattgtc gtatgtattt gccatttcgg ggttaagtcg
      121 caatgcttgt tcgtaatcgc ctactgcacg gtctatatcg cccatcattc ggcgagcact
      181 gccacggtcg ttgtatgcat atgcgtagcg agggtttagc tgaatagcat tggtataggc
      241 acttacagca cctttgtagt cctttataaa aaacatacat actccttgat aataatatgc
      301 ttcggcagtt tcatattgta acaatatagc tttggtaaca tcgcgcaagc atgacataaa
      361 atcgtttttg ctaaagtaca ccatagcacg cgaataccac acttgtgcta ttgttgaatc
      421 tactcctaat acataattgt aagctgatat agcaccatca agatcgttag tatatgtttt
      481 ggcagctccc aagtttatat aagcttttaa aaactgagga ttgtaacgta ttgctaactc
      541 aaactgttgt attgcttgtc cgtattgttg tgcgttaagc aattgaatgc cattgttata
      601 tgctaattct gcttcttgat tttgttctat aactatggta tttatagttt ctgtttgtgc
      661 attaagcata atgtgtgcag cgcaaaataa aaaacttgta atgagtaatt ttttgaaata
      721 catataatta ttgttttata aggtatattg ttcaaaagta aaaaaaatat ttgtataatt
      781 cttaattttt aattcttaat ttttaattaa atcagggtat aaggtttaca atgatattca
      841 aattactatg atacaagtaa ttatttgtaa ttgaaaataa ggaatttggg agtacttttt
      901 gttttatagt ttatgattgt accattaagt tgcatcctcg tatatcacta ctgtcgatac
      961 gaccttcaac actaaatgta ttggtatgta aaattctccc tatatcttgt gtttcaataa
     1021 aagcacacga ataccaattg gctaagtcaa ttatattgat tccgccatga gttccatttg
     1081 gaacataata acgagggtcg tacatatctc gaatcaaaac cttcatccaa ggagcaggtg
     1141 taaattcacc gttgcgtaac gcgtaagctt gacttaataa ttctgtcatt ccatattcgg
     1201 aatgaatgtg agacactcca aaagattttt gtaaaatttc atgaacttca gaacgaatca
     1261 tctctttttt acgtcctttc ataccgcctg tttccattac aattaaattg ttatgattac
     1321 atgtatattg ttcggcaaaa tcaagtaaag caaatgtaac acctattaga aaaatttttg
     1381 aatgtgaata ggtttggata gcattatgta aatcgtcaaa attatgaagg taaaatccgc
     1441 ttgccgtatt ttgtgattct tgtattaaat gttgtatcat atataccaat gaagaattag
     1501 aacgctctaa atatgaaggt aataatgcca aaaaaatata ttcctgcgga ttaccatata
     1561 ttcgtttaaa catagtcata aaactttggc ggtaaatatt gagcgaaggt atataatgtt
     1621 tacttgcgat agtgccggta gttttggaac tttcaaaaat tgtttctacc ggtaatgacg
     1681 acgatacaac agtatgtgat ttaaaaaaag aaataggtaa acaaggaatt tgtgaatagt
     1741 gagttatagt atgcggttgt atgcccaaat gttttatgaa tgaagcatat acagtacaat
     1801 gaacagattg gtattgaaat aatctgatag catactcatt aaatgtattt tcagaatcaa
     1861 ttgtaaatat gcttgaatca aaattgggga tcatatttca tttttttaca aagatattct
     1921 ttttatcaca tttgtaataa tgtgtatttc cccttgtaat attttcaaac aaattatgtc
     1981 aaattatatg tataaatatt acatgtatat gaaattgttg gcacatatca tacttttaat
     2041 cgtaatgatg aactgtagcg gcaattcaca ggtaattctg caagaacaaa aaaatgatac
     2101 tattatgaaa tatcgtacat taaatgaatt tgaacaatat gtaattttga ataaaggtac
     2161 agaacgcccc tttagcggag aatatactga tttgtttgaa gaaggagtat atgcgtgtaa
     2221 acaatgtggt gcagaattgt atac
//
LOCUS       DAOTOW010000373         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37779_length_2240_cov_3.313959, whole genome shotgun sequence.
ACCESSION   DAOTOW010000373 DAOTOW010000000
VERSION     DAOTOW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37779_length_2240_cov_3.313959"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="P5243_07120"
     CDS             <1..817
                     /locus_tag="P5243_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS19257.1"
                     /translation="AKTLAIQTLATSIKASCNRIQFTPDLLPADLLGTMIYSQKNEEF
                     VVKKGPIFAHFILADEINRAPAKVQSALLEAMQERQVTIGSHSYALEEPFLVMATQNP
                     IEQEGTYPLPEAQVDRFMLKIIVDYPSKEEEQRIIRQNLLKVFPKASSVISIEQIIKA
                     REVVTEVYMDEKIEKYIVDILFATRNPQQYNLSHLQHLISYGCSPRAGISMAKAAKAY
                     AFIKRRGYVIPEDVRAVCHAVLRHRIGLTYEAEAENVSVESIITQVINAVDVP"
     gene            872..1738
                     /locus_tag="P5243_07125"
     CDS             872..1738
                     /locus_tag="P5243_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRS19258.1"
                     /translation="METSELLQRVRKIEIKTRGLSNTLFAGEYHSMFKGRGMAFSEVR
                     EYQYGDAIRDIDWNVTARFNKPYVKVFEEERELTVMLVVDVSASHSIGTRGQIKRNCM
                     TEIAAVLAFSALQNNDKVGLLLFSDTIETFIPPQKGRKHILRIIRELLERETTKTETN
                     IPLALQYITNAIKRKCTAFILSDFIDSHPIIDALSIANRKHDIIAIRVYDAMEMSLPD
                     VGLIRIHDVESQSQAWIQTSDATVRKIYAQNYTERVQYLQSVFVKSGVDYVHVQSDKD
                     YVKPLLNLFKKR"
     gene            1738..>2240
                     /locus_tag="P5243_07130"
     CDS             1738..>2240
                     /locus_tag="P5243_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19259.1"
                     /translation="MKQLRLLFIVVWFLPISLFSQGYSVQMSVDTNRIGLGERTHITL
                     HFTESKSSKYTLPAYGQELIKNIQLCSMPEYDSVEIQKDSIQIICKYCITSFVPGEYS
                     FQIGPIIHNNKDTIWLPTIGLSVVADTVDLQGEIRDVAPIQTPQYTWFERNKQWIVWG
                     VVVLGVIL"
BASE COUNT          788 a          340 c          408 g          704 t
ORIGIN      
        1 agcaaaaaca ttagctatac aaacgttggc tacatctatc aaagcttcgt gtaatcgtat
       61 tcaatttacc cccgatttat taccggctga tttattgggg acaatgattt atagccaaaa
      121 aaatgaagaa tttgttgtaa aaaaagggcc aattttcgct cattttattt tagcagatga
      181 aataaaccgt gcaccggcaa aagttcaaag tgctttgctc gaagctatgc aagagcgaca
      241 agtaacaata ggaagtcata gttatgcact tgaagaacct tttttggtta tggctactca
      301 aaatcctata gaacaagaag gaacgtatcc tttaccggaa gctcaagtag acagattcat
      361 gcttaaaata atagtagatt atccgtcaaa agaagaagaa caacgaataa ttcgtcaaaa
      421 tttattaaaa gtttttccta aagcatcatc tgttataagt atagaacaaa ttattaaggc
      481 tcgcgaagtt gtaacagaag tatatatgga tgaaaagata gaaaaatata ttgtagatat
      541 tttatttgca acacgtaatc cccaacaata taatctttct catttacaac atttaatatc
      601 atacggatgt tcacctcgtg ccggaattag catggcaaaa gcagcaaaag catatgcgtt
      661 tataaaacga agaggatatg taatacccga agatgtaaga gcagtatgtc atgcagtact
      721 tcgtcaccga attgggttaa cgtatgaagc cgaggcagaa aatgtgagcg tagaatctat
      781 tattactcaa gtaattaatg cagttgatgt accatagttt tttaatgaat attataacaa
      841 ggtagttacg cattacattg atactgtaca catggaaacc tcagaattgc tacagcgagt
      901 tcggaaaatt gaaataaaaa ctcgtggtct ttcaaataca ttgtttgccg gagaatatca
      961 cagtatgttc aaagggcgag gaatggcttt tagtgaagtt cgtgaatatc aatacggtga
     1021 tgctattcgt gatatagact ggaatgttac tgctcgtttt aataaaccct atgtaaaagt
     1081 atttgaagaa gaacgtgaac ttacagttat gcttgtagtt gacgttagtg cttcgcattc
     1141 tattggcact cgtggtcaaa taaaacgaaa ttgtatgact gaaatagctg cagtattggc
     1201 tttttctgca ttacaaaaca atgataaagt agggttgctt ttattttctg atactattga
     1261 aacatttatt cctcctcaaa aaggaagaaa acatatatta cgaataatac gtgaactttt
     1321 agaaagagaa acaactaaaa ccgaaactaa tataccatta gctttgcagt atattacaaa
     1381 tgcaattaaa cgcaaatgta cagcatttat actttctgat tttattgatt ctcatcctat
     1441 tatagatgct ttatcaattg caaacagaaa acacgatata attgcaatac gggtatatga
     1501 tgctatggaa atgagtttgc cggatgtagg gcttattcgt attcatgatg tagaatctca
     1561 atcgcaagca tggattcaaa cgtctgatgc aacagtaaga aaaatatatg cacaaaatta
     1621 tactgaacga gttcaatatt tacaatcagt gtttgtaaaa tcaggagttg attatgtgca
     1681 tgtacaaagt gataaagatt atgttaaacc attgttgaat ttatttaaaa aacgataatg
     1741 aagcagttgc gtttgttatt tatagtagtt tggtttttac ctatatctct tttttctcaa
     1801 ggatattctg ttcagatgtc tgttgatacc aatagaatag ggttaggtga acgaactcat
     1861 ataacacttc attttacaga atcaaaatca tcaaaatata ctttacctgc atatggccaa
     1921 gaacttataa aaaatataca attgtgttct atgccggaat atgatagtgt tgaaattcag
     1981 aaagattcaa ttcagataat ttgtaaatat tgtattactt cgtttgttcc cggtgaatat
     2041 tcatttcaaa taggtccaat tattcataac aacaaagata ccatatggtt gccaactatt
     2101 gggttatcgg tagtggcaga tactgttgat ttgcaaggtg aaatacgcga tgtcgctcct
     2161 attcaaacac ctcaatatac ctggttcgaa cgaaataaac aatggatagt gtggggtgtt
     2221 gttgtactag gagttattct
//
LOCUS       DAOTOW010000374         2237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_37862_length_2237_cov_3.068744, whole genome shotgun sequence.
ACCESSION   DAOTOW010000374 DAOTOW010000000
VERSION     DAOTOW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37862_length_2237_cov_3.068744"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="P5243_07135"
     CDS             complement(<1..1030)
                     /locus_tag="P5243_07135"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRS19260.1"
                     /translation="MSVYSSFIRPVLFLFKPETVHNFTIEYLHILSKIPFASAIIRKA
                     YPIPQSKELERTVCGITFPHPVGLAAGLDKNAKAYKMFGAMGFAFVEVGTVTPKAQPG
                     NPLPRLFRLPKDKAIINRMGFNNYGVQYMVRKLKKRNTNIIIGGNIGKNKVTPNENAT
                     ADYVTCFEALYDYVDYFVVNVSSPNTPNLRELQDKKPLTELLETLMYENKQKPKQKPI
                     FLKIAPDVTHSQLDDIIEIVTKTKIAGIVATNTTITRNNLTYSKEYIENIGAGGLSGA
                     PETAMSTEVIRYLHTKSNGAFPIIGVGGIMTPEDAYEKLQAGASLIQLYSGFIYEGPS
                     LIQRICYYI"
     gene            complement(1035..1928)
                     /gene="era"
                     /locus_tag="P5243_07140"
     CDS             complement(1035..1928)
                     /gene="era"
                     /locus_tag="P5243_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRS19261.1"
                     /translation="MNKLQHKSGFVNIVGNPNVGKSTLMNELVGERISIITNKSQTTR
                     HRIMGIVNTDDYQIVFSDTPGVLKPLYKLQESMRKFSESALVDADVMLYITDIYETPD
                     KNEDFLQKVAKLSSPILLIINKIDLTNQEKLTTIVQTWEQRLPHAEIIPCSALTKFNV
                     QYILKRIVELLPIGPPYYDKDALTDKPLRFFVSEIIREKILLYYQKEIPYSTEVVIEE
                     YKEEPTRDIIRAVILVNRDSQRGIVIGNKGAAIKKLGIESRLEIEKFVQKKVHLELFV
                     KVSKDWRDNESLLKQFGYNIL"
     gene            complement(1921..>2237)
                     /locus_tag="P5243_07145"
     CDS             complement(1921..>2237)
                     /locus_tag="P5243_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRS19262.1"
                     /translation="HAFLDKNLKLDDFQSISEGVELEDGLVEVDDLQYVNAQDKSQIG
                     IQIHSGKNHVVKRIFEKFGYTVEKLDRVYFAGLTKKNLPRGKWRFLTRAEVAILKQGA
                     YE"
BASE COUNT          690 a          398 c          331 g          818 t
ORIGIN      
        1 atatataata acaaattctc tgtatcaaag aaggtccttc gtaaataaag ccactataca
       61 attgaatcaa acttgctcct gcttgcaatt tttcgtatgc atcttctgga gtcataattc
      121 ctccaacacc aatgataggg aatgcaccat ttgatttggt atgtaaatac cgaattactt
      181 cagtagacat agctgtttca ggtgctccac taagccctcc tgctccaata ttttcaatat
      241 attcttttga atatgtaaga ttattacgag taatagtagt attagttgca actataccgg
      301 caatttttgt ttttgtaact atttctatta tatcatctag ttgactatgt gttacatctg
      361 gtgctatttt taagaaaatt ggtttttgct ttggtttttg cttattttca tacattaatg
      421 tttcaagcaa ttctgtcaat ggttttttat cttgcaattc acgtaaatta ggcgtattag
      481 gtgaacttac atttaccaca aaataatcta cataatcata caatgcttca aaacaagtaa
      541 cataatcagc tgtagcattt tcattgggag taactttatt ttttccaata ttacctccta
      601 ttattatatt agtgttacgt tttttgagtt tacgtaccat atattgcaca ccgtaattat
      661 taaatcccat acgatttatt atagctttat cttttggtaa tctgaataaa cgcggaagcg
      721 gattgcccgg ttgagctttg ggggtaacag tacctacctc aacaaatgca aaccccatag
      781 caccaaacat tttgtaagct ttggcatttt tatcgagtcc cgcagccaaa ccaacagggt
      841 gaggaaaggt aattccacac acggtacgtt ctaactcttt tgattgaggt ataggatacg
      901 ctttacgaat aattgcagaa gcaaatggaa ttttactcaa tatgtgtaaa tactctatag
      961 taaaattatg aactgtttcc ggtttaaaca aaaataatac agggcgaata aatgatgaat
     1021 atactgacat acgcttataa tatattataa ccaaattgtt taagcaacga ttcattgtcg
     1081 cgccaatctt tacttacttt aacaaataat tctaaatgaa cttttttttg tacaaatttt
     1141 tctatttcca aacgtgattc tatacctaat tttttgattg cagcgccttt atttcctatt
     1201 acaattcctc gttgcgaatc tctgtttact aaaattacgg cacgtattat atctcgtgtg
     1261 ggttcttctt tgtattcttc aattaccact tctgtagaat acggtatttc tttttgataa
     1321 tacaataata ttttttcgcg tatgatttcc gaaacaaaaa aacggagcgg tttatcggtc
     1381 aacgcatctt tatcataata tggcggacct ataggaagca attctactat ccgctttaaa
     1441 atatattgaa cattaaattt agtaagtgcc gaacatggaa taatttcggc atgcggtaag
     1501 cgttgctccc atgtttgaac tattgtggta agcttttctt gatttgtaag atctatttta
     1561 ttgataataa gcaatattgg cgatgaaagt tttgctactt tttgcaaaaa atcttcattt
     1621 ttatcgggag tttcataaat atctgttata tacaacatta catctgcatc aaccaacgcc
     1681 gattccgaaa atttacgcat agactcttgc agtttataca atggttttaa aacacccggg
     1741 gtatcagaaa acacaatttg gtagtcatcg gtatttacaa ttcccattat tctatggcgg
     1801 gtagtttgcg atttattggt aattattgaa attctctcgc caaccaattc attcatcaag
     1861 gtagattttc caacatttgg attacctaca atattaacaa aacctgattt atgttgtaat
     1921 ttattcatac gcaccttgtt ttaatatagc aacttcggca cgtgttaaaa atcgccattt
     1981 accacgtggt aaattttttt tggttagacc tgcaaaataa actctatcta atttttcaac
     2041 tgtatatccg aatttttcga aaattcgttt taccacatga tttttgcccg aatgaatttg
     2101 aattcctatt tgcgatttat cctgagcatt tacatattgt aagtcatcaa cttcaaccaa
     2161 tccatcttct aattcaacac cttctgaaat agattgaaaa tcgtcgagtt taagattttt
     2221 atctaaaaaa gcatgat
//
LOCUS       DAOTOW010000375         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38059_length_2228_cov_3.087897, whole genome shotgun sequence.
ACCESSION   DAOTOW010000375 DAOTOW010000000
VERSION     DAOTOW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38059_length_2228_cov_3.087897"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="P5243_07150"
     CDS             <1..850
                     /locus_tag="P5243_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19263.1"
                     /translation="TCSVKVTTNIPQSNIYNDSEFTFSANWNVETGLTDGRYMGDEHY
                     TFTSGAYYTFTFYGNQFEIWGTKDAHHGLANVSIDNGTPVSISYKASTRAVNQLIWTS
                     PILESGTHTIKVTAASSSVIVADKIVVYNSIQSLSQTISLKKGWNLISTNIQLQDSTI
                     ETVFAGLDVQMVKDVNGFWKKGQNTAFNSLKTITPGKGYLVNMNAAGTLTITGIPSTS
                     VLQYAPTTGWQLIGSPFQTTTPFATYFNASNCQTIKNFDGFWQPNGTVNSITNLEPGK
                     GYFLKK"
     gene            complement(856..>2228)
                     /locus_tag="P5243_07155"
     CDS             complement(856..>2228)
                     /locus_tag="P5243_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19264.1"
                     /translation="YLCYIIIKHCPKNGKLTSVVVSIASVDVVPIVESVLELHTELLN
                     RKQIKVIKKISDITFVSTDTNMLHTIIRNLLSNAIKYSYEQSSITISISKHDIRYTAI
                     TISDTGMGMSEDRLQEIFSVKKHSYMGTANEQGTGLGLVIVKEFVSLLQGAITVTSKE
                     GEGTTCSILIPSATEQTMQAYEETFPTIRMQTKHNSASTDDTKKYVQHKHILIVDDDD
                     AMRLSLRLLLEEYATIHEASSGDEAYMLLLETHPDLVISDIQMMHGGGIELCEKIKEN
                     ASVSHIPIILLTAFGTETDMIEGLKAGADEYLSKPFNKEILLLKIQYVFKLREELHRM
                     FRIQEIVEVQSGTINSLDEKIIQKIITYIHEHIADPNLSVEQLSDFVGASRSTLHRKM
                     NALTNMTPNDFIRMIRLKKAAELLKTGKFSISEVAYDTGFNDPRYFSKCFKEFFGKTP
                     SEFTKE"
BASE COUNT          700 a          413 c          384 g          731 t
ORIGIN      
        1 aacttgctcg gttaaagtta caacgaatat tcctcaatca aacatctata atgattcaga
       61 atttaccttt tctgcaaatt ggaacgtaga aacaggatta actgacggac ggtatatggg
      121 cgatgaacac tacacgttta catcgggagc ttattatacc tttacatttt atggtaatca
      181 atttgaaata tggggaacaa aagatgctca tcatggctta gccaatgtgt ctattgataa
      241 tggtactccc gttagtataa gttataaagc atcaactcgc gcagtaaatc aattaatctg
      301 gacaagtcca atactggaat caggaactca taccattaaa gttacagcag catcctcaag
      361 tgtaatagta gcagataaaa tagttgtgta caatagtatt caatctttga gtcaaaccat
      421 atccctcaaa aaaggctgga atttaatttc tacaaatata caattacaag acagcaccat
      481 agaaacagta tttgcagggc tcgatgttca gatggtaaaa gatgttaacg ggttttggaa
      541 aaaagggcaa aatacagcat tcaattcgct taaaaccata acacccggca aaggatattt
      601 ggtgaatatg aatgctgcgg ggacgctaac aattacagga atcccatcta caagcgtatt
      661 gcaatatgcc ccaacaacgg gttggcaatt aatcggcagc ccgttccaga ccacaactcc
      721 attcgctacc tactttaacg cttcaaactg tcaaacaatc aaaaattttg atggattttg
      781 gcaaccaaat ggaacggtaa attctataac caatttagaa ccgggtaagg ggtatttttt
      841 gaagaaataa tgaatttatt ctttggtaaa ctccgatgga gttttaccaa agaattcttt
      901 aaaacatttg ctaaaatatc tcgggtcgtt aaatcctgta tcgtaggcaa cttctgatat
      961 agaaaattta cctgttttca acaattcagc tgcttttttg aggcgtatca tacgaataaa
     1021 atcgttggga gtcatattgg ttaatgcatt catttttcga tgcaaggtag aacggcttgc
     1081 tccaacaaag tcgctcaatt gttctaccga aagatttgga tcggctatat gttcatgaat
     1141 atatgtaata atcttttgaa taattttttc gtccaatgaa tttattgtac cactttgcac
     1201 ctcaacaatt tcttgtatcc gaaacattct gtgaagttct tcgcgcagct taaacacgta
     1261 ttgtattttg agtaataata tttctttgtt gaatggtttc gacaaatatt catcggcacc
     1321 ggctttgagc ccctcaatca tatcggtttc agtaccaaat gctgttaaca atataatggg
     1381 aatatgcgat accgatgcat tttctttaat tttttcacac aattcaatcc ctccaccatg
     1441 catcatttga atatcactta taactaaatc cggatgtgtt tcgagcaata gcatatatgc
     1501 ttcatcgccg ctacttgctt catgaattgt tgcatattct tccaaaagca atcgcaagga
     1561 caaacgcatt gcatcatcat cgtcaactat caatatgtgt ttgtgctgca catacttttt
     1621 ggtatcgtcg gttgacgctg aattgtgttt ggtttgcatg cgtatagttg gaaatgtttc
     1681 ttcatatgct tgcatagttt gttccgttgc cgatggtatc aaaatcgaac atgttgtgcc
     1741 ttcaccctct ttactcgtta ctgtaatggc accttgcaaa agcgacacaa attctttgac
     1801 tataactaac cctaagcctg taccttgctc gttggcagta cccatatatg aatgtttttt
     1861 tactgaaaaa atttcttgta atctgtcttc tgacattccc attccggtat cggatatagt
     1921 tattgcagta taccgtatat cgtgttttga aatagaaatt gtaattgatg attgctcgta
     1981 tgaatatttg attgcatttg acaataaatt tctaataata gtatgtaaca tattggtatc
     2041 ggtagaaaca aacgttatgt cagatatttt ttttataact ttaatttgtt tacgattcaa
     2101 caattcggta tgtaattcta gtactgattc aacaataggt actacatcaa ccgaagcaat
     2161 tgacacaact accgatgtaa gcttcccatt tttaggacag tgtttaatta taatgtaaca
     2221 aagatatt
//
LOCUS       DAOTOW010000376         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38062_length_2228_cov_2.971928, whole genome shotgun sequence.
ACCESSION   DAOTOW010000376 DAOTOW010000000
VERSION     DAOTOW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38062_length_2228_cov_2.971928"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1814
                     /locus_tag="P5243_07160"
     CDS             <1..1814
                     /locus_tag="P5243_07160"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRS19265.1"
                     /translation="LESSKGITFKIDACENKPVSKSPAPPFTTSTLQQEAGRKLGFPV
                     AKTMSVAQKLYEEGHITYMRTDSVNLSDIAIATAKSFIVETYGKEYSKIRKYTTKTKG
                     AQEAHEAIRPTYIKNEVVSANSDEQRLYELIRKRTIASQMADAQIARTVFTINCKNDT
                     SAYFSAKGEVVTFDGFLKVYMESSDDDSVEEQEITLPPLSIGTMLNLETMNAIERFTQ
                     HPPRYTEATLVKKLEELGIGRPSTYAPTISTIQKRGYVIKEDRIGKKRKVISIELHNN
                     TISKTIKTEFFGSEKSKLFPTDIGMVVNDFLVKYFGQILDYNFTAQVESQFDEIAEGN
                     LQWQKMIHNFYTSFHSTVEKTIEESERGDGERLLGIDPKTGKQVFVRIGRFGPMVQLG
                     TTESDEKPIYASLPKGQLIETITIDDAIELLYNNNDGNELGVDLQTGKKVYVKSGRFG
                     PFVQLGESDDPEKKYVSLLKGQKPETITLEQALTLLKLPRYLGEFENKKVEVNIGKFG
                     PFISHNSTFTSLKKSDPDVFVITLEQAIDCILKKRENDVAKSIKIFDEDKKIKIVFDR
                     WGKPCVFYKKRFFRITKNVNPESLTLQQCYEIAGEKK"
     gene            1821..>2228
                     /locus_tag="P5243_07165"
     CDS             1821..>2228
                     /locus_tag="P5243_07165"
                     /inference="COORDINATES: protein motif:HMM:NF037045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide phosphoribosyltransferase
                     domain-containing protein"
                     /protein_id="HRS19266.1"
                     /translation="MKSLILKSDSYKFTHHPQYPKGTTKVGSYLESRGGLFDNTVFYG
                     LQYILKKHFEGVVVTKQDVEKAKKIIDAHIGPNIFNYDGWMRIVDVHGGKIPIQIKAV
                     SEGTVLKTSNVLMTVENTDDQLPWITNFCESILL"
BASE COUNT          868 a          316 c          396 g          648 t
ORIGIN      
        1 ttttagaaag ttccaaagga attacattta aaatagatgc gtgtgaaaat aaaccggtat
       61 caaaatcacc ggctccacca ttcacgactt ctactttgca acaagaagcc ggaagaaaat
      121 taggatttcc ggttgcaaaa actatgagcg tagctcaaaa actctatgaa gaagggcata
      181 ttacttatat gcgtacagat tctgttaatc tgtcagatat agcaattgct actgctaaaa
      241 gttttattgt ggaaacgtat ggaaaagaat atagtaaaat tcgaaaatat accactaaaa
      301 caaaaggggc tcaagaggca catgaagcta ttagacctac atatattaaa aatgaagtag
      361 tttctgctaa ttcagatgaa caaagattgt acgaattgat tcgaaaacgc actattgcct
      421 cgcaaatggc cgatgctcaa atagcaagaa cagtatttac aataaattgc aaaaatgata
      481 cgtcagctta tttttcggca aaaggtgaag ttgttacttt tgatggattt ttaaaagtat
      541 atatggaatc ttctgatgat gattctgttg aagaacaaga aataacgtta cctccattat
      601 caataggtac aatgttgaat ttagaaacga tgaatgcaat agaacgattt acgcaacatc
      661 ctccacgata taccgaagca acacttgtta aaaagttaga ggaacttggt attggtcgtc
      721 cttcaacata tgcacctaca atttcaacta tacaaaaaag aggttacgta ataaaagagg
      781 atagaatagg taagaagcgt aaagttattt ctattgaatt acacaataac actatatcta
      841 aaactattaa gacagaattt tttggttcag aaaaatcaaa actttttcca acggatatag
      901 gaatggtggt aaatgatttt ttagttaaat attttggaca aattttagat tataacttta
      961 ctgctcaagt agaatctcaa tttgatgaaa ttgctgaagg aaatttacaa tggcagaaaa
     1021 tgatacacaa tttttataca tcatttcatt ccactgtaga aaaaactatt gaagaatcag
     1081 aacgaggaga tggagaaaga cttttaggaa ttgatccaaa aaccggtaaa caagtatttg
     1141 ttagaattgg tagatttggg ccaatggtac agttagggac tacggaatca gatgaaaaac
     1201 caatctatgc aagtttaccc aaaggacaat taatagaaac tattacaatt gacgatgcaa
     1261 ttgaactact ttataataat aatgatggaa atgaattagg tgttgattta caaaccggta
     1321 aaaaagtgta tgtaaaatca ggtagatttg gaccttttgt gcaattagga gaatcagatg
     1381 atcctgaaaa aaaatatgtg agtttattaa aaggacaaaa accagaaaca ataaccttag
     1441 aacaagcact tacattatta aaattacctc gatacttagg agaatttgaa aataaaaaag
     1501 ttgaagttaa tataggaaaa tttggaccat ttatttctca taattctacg tttacatcct
     1561 taaaaaaatc agatccggat gtgtttgtga ttactttaga acaagctatt gattgtattt
     1621 tgaaaaaaag agaaaatgat gtagcaaaaa gtataaaaat atttgatgaa gataaaaaga
     1681 ttaagatagt ttttgataga tggggaaaac catgtgtgtt ttataaaaaa cgatttttca
     1741 gaattaccaa aaacgtaaat ccggaatcat taacgttaca acaatgttat gaaattgccg
     1801 gagaaaaaaa ataatataat atgaaaagtc taatactaaa atcagattcc tacaaattta
     1861 ctcaccatcc tcaataccca aaaggtacaa ctaaggtagg ctcatacctt gaatctcggg
     1921 gtggtctatt tgacaacact gttttctatg gtcttcaata tattcttaaa aaacattttg
     1981 agggggttgt agtcacaaaa caagatgttg aaaaagctaa gaaaattatt gatgcccata
     2041 ttggaccaaa tatattcaac tatgatggtt ggatgaggat tgtggatgtt catggtggaa
     2101 aaataccaat tcaaattaaa gccgtgtcgg aaggcacggt tctaaaaacg agtaacgttc
     2161 tcatgactgt tgagaacaca gatgaccagc taccttggat caccaatttc tgtgagagta
     2221 tccttcta
//
LOCUS       DAOTOW010000377         2225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38108_length_2225_cov_4.080645, whole genome shotgun sequence.
ACCESSION   DAOTOW010000377 DAOTOW010000000
VERSION     DAOTOW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38108_length_2225_cov_4.080645"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="P5243_07170"
     CDS             <1..952
                     /locus_tag="P5243_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS19267.1"
                     /translation="EIGIEHIFSFKTKESNALEYLSAKEIAVCDEKRELFFSMLISAM
                     ANSTGGTIFIGIHAIRKIPKNIEPITNEKAIEWLTHICNTSISPIIPNLNIQKIFVSP
                     SNYIIGIHVPNSSKAPHMCIDKRFYKRTNLTQTVLEEYEIRDLYTKSKRSEIELYSIM
                     NTNGIPMLSGGKFQYINFYPRFLIKNTGTTVERFFKVEIWVPSQINNPNFDSLQNHFS
                     RFEDGHSVFSITHKTPFFQNEIATVAEANFIVDSHNFDAFEHGEITIKLFFSNGIETK
                     IFRCKELLLYKKKQLELSDFAHAQTVIETPVQPEQPRLFM"
     gene            954..1160
                     /locus_tag="P5243_07175"
     CDS             954..1160
                     /locus_tag="P5243_07175"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRS19268.1"
                     /translation="MKWVTIQTFTYAHEAYIAQSKLESFGIETFLQDELTTQIIPVYS
                     YATGGVKLQVHEQDVEKALKILEL"
     gene            complement(1155..1616)
                     /locus_tag="P5243_07180"
     CDS             complement(1155..1616)
                     /locus_tag="P5243_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS19269.1"
                     /translation="MQVLKQVTVLFVICLVANISMFAQEQTPSIFEILVQPMAGYGEV
                     TIKQDERLVTLLNNHKALNIQNESKIQGWRVQIYNSSGVDSRKEAEEIRRKFLERYPD
                     ATVYVIYQPPFFKIRVGDFRTREDAYMLYKQIVNEFPISYLVNDIIQLPKL"
     gene            1721..>2225
                     /locus_tag="P5243_07185"
     CDS             1721..>2225
                     /locus_tag="P5243_07185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS19270.1"
                     /translation="MKKLCIVYIIILLFQFSLFAQEIVSGAKFGTQIGNIAPNIELPS
                     NDGTIIDLYSLRGSLVLVDFWASWCGPCRAENPNLVKAYEAFKNKSFQDGNNFVIYSV
                     SIDTNPTWWYNAIQRDNLQWNTHVIDTRGWNSPYILMYNIQGIPANFLVNADGIIIAK
                     NIRGASLY"
BASE COUNT          784 a          355 c          328 g          758 t
ORIGIN      
        1 cgaaattggc atagaacata tcttttcttt taaaacaaaa gaaagtaatg cattagaata
       61 tttatcggct aaagaaatag ctgtgtgcga tgaaaaacgc gaattatttt tttcaatgct
      121 cataagtgct atggcaaatt ctacaggcgg tactatattt attggcatac atgctattcg
      181 caaaatacct aaaaatatag aacctattac caatgaaaaa gccatagaat ggttaactca
      241 tatttgcaat acatctatta gtccaattat tccaaatctt aatattcaaa aaatatttgt
      301 ttctcctagt aattatataa ttggaattca tgttcctaat agcagtaaag cgccgcacat
      361 gtgcatagat aaacgctttt ataaacggac aaacctaaca caaactgtac ttgaagaata
      421 tgaaattcgc gatttatata ccaaaagtaa gcgcagcgaa atagaattat attctattat
      481 gaacacaaat ggaattccaa tgctttcggg gggtaaattc caatacatta atttttatcc
      541 tcgtttttta attaaaaata caggtactac tgttgaacgc ttttttaaag ttgaaatttg
      601 ggtgccatca caaataaaca acccaaattt tgattcatta caaaaccatt tttcacgatt
      661 cgaagatggt cattccgttt ttagtattac tcataaaaca cctttttttc aaaatgaaat
      721 tgcaacagtg gccgaagcaa attttatagt agattctcac aattttgatg cttttgaaca
      781 tggagaaatt acaataaaat tgttttttag taatggtatt gaaacaaaaa tttttcgatg
      841 caaagaacta ttgttataca aaaagaaaca attagagtta tcggattttg cacatgcgca
      901 aacagtaatt gaaacacccg tgcaaccaga acaacctcgt ttatttatgt aatatgaagt
      961 gggttactat tcaaacattt acttatgctc acgaagccta tatagctcaa tcaaaattag
     1021 aaagttttgg aattgaaact tttttacaag acgaacttac cacacaaata attcctgtat
     1081 atagctatgc caccggggga gtaaaactcc aagttcacga acaagatgta gaaaaagcac
     1141 taaaaatatt agaattataa tttgggtaac tgtattatat cattcaccaa atatgatata
     1201 gggaattcat ttacaatttg tttataaagc atatacgcat cttcacgagt tctaaaatcg
     1261 ccaacgcgta ttttaaaaaa aggtggttga tatatcacat acacagttgc atcaggatat
     1321 cgttcaagaa attttcttcg aatttcttct gcttcttttc gtgaatcaac acccgaagaa
     1381 ttgtaaattt gtactcgcca accttgtatt ttactttcgt tttgaatatt gagtgcctta
     1441 tgattattaa gcaaagttac taatctttca tcttgtttta tagttacttc tccatatcct
     1501 gccattggct gaacaagtat ttcaaaaata gatggagttt gttcttgtgc aaacatagaa
     1561 atatttgcca ctaaacatat tacaaacaat acggttactt gtttcaaaac ttgcatttgt
     1621 tttaccattt ctttgcacaa aaatagttat ttttttattg taataaacac tatactattc
     1681 tttatatttt tgcgatattt gaaatactaa attacatgtt atgaaaaaac tttgtatagt
     1741 ttatattatt atactactgt ttcaattttc gctttttgca caagaaatag tctctggggc
     1801 aaaatttgga acacaaatag gaaatattgc acctaacatt gaattacctt caaacgatgg
     1861 aactattata gatttatatt ctttacgagg ttctttagta ttagttgatt tttgggcatc
     1921 gtggtgtggt ccatgtcgtg cagaaaatcc aaacttagta aaagcttatg aagcgtttaa
     1981 aaataaatca tttcaagatg gaaataattt tgtaatttac agtgtttcta ttgatactaa
     2041 tcctacatgg tggtacaatg caatacaacg cgacaattta caatggaaca cccatgtaat
     2101 agacacccga ggttggaata gcccatacat tcttatgtat aacatacaag gtatacctgc
     2161 gaatttttta gtaaatgctg atggtattat aattgcaaaa aacattcgcg gtgcctcttt
     2221 atatg
//
LOCUS       DAOTOW010000378         2220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38222_length_2220_cov_3.100693, whole genome shotgun sequence.
ACCESSION   DAOTOW010000378 DAOTOW010000000
VERSION     DAOTOW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38222_length_2220_cov_3.100693"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="P5243_07190"
     CDS             complement(<1..1190)
                     /locus_tag="P5243_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19271.1"
                     /translation="MKTIFSISIVSMITLVSCSSWEALNAQYQDDVYSPRQQYEVTST
                     PSNTNQTKPVYVDGTLDPQVYNDNFVQKQEEQQNYDYQNQDNSYDKEPTIVNNYYGNV
                     YSDDYYYSRRFFDTYTPSYYYDNYWWYPDWSFSINIGDPYWSISYRPYWGWGCRYYRP
                     YYSGYYYGYGYGYPYSYYRYYDYPYYYSYPRYYSSKPRYDSYGPSSSYSSRRSQAESR
                     PSNSYSGSSKDGSTYDNSRTRTSTPSSSPSTRSYQSTDRPENASPRGSYESKSYSNEQ
                     SGSTQTTTRTNSPSSSQSGRTSAEQSRPAGGERPNNPTQPNYKMEQPTVPSQRNSEFV
                     PPARPNSQPQNQPQRVQPNSSRYDNQSSGYKSESNTNTSVNREYNSNQTSRSYNSDYN
                     QSQQV"
     gene            1292..>2220
                     /locus_tag="P5243_07195"
     CDS             1292..>2220
                     /locus_tag="P5243_07195"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662800.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRS19272.1"
                     /translation="MAKELTKRSENYSQWYNDLVVKADLAENSAVRGCMVIKPYGYAI
                     WEKMQAQLDSMFKATGHVNAYFPLFIPKSFFSKEASHVEGFAKECAVVTHYRLKNDPE
                     GKGIIVDENAKLEEELIVRPTSETIIWNTYKNWIQSYRDLPILCNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATKQEAMEEAEKMIRVYADFAENFLALPVIMGAKTANERFA
                     GALETYTIEALMQDGKALQSGTSHFLGQNFAKAFDVTFTTKEGTLDYVWATSWGVSTR
                     LMGALIMAHSDDNGLVLPPKLAPIQVVIVPIYKG"
BASE COUNT          726 a          349 c          392 g          753 t
ORIGIN      
        1 acttgttgag attgattata gtctgaatta taacttctcg aagtttggtt tgaattatac
       61 tctctattta cacttgtatt agtatttgat tccgatttat atcctgaaga ttgattatca
      121 taacgcgacg aatttggctg aacacgttgc ggttgatttt gcggttgact attaggtcta
      181 gctggaggca caaactcact atttctttga gatggcacag taggttgttc cattttgtaa
      241 ttaggttgtg tcggattatt aggtctttca ccacctgccg gtcttgactg ttctgcactt
      301 gttctacccg attgtgaact acttggcgaa tttgttctag tagttgtttg tgtagaccct
      361 gattgctcat tagaatatga tttactttca tagcttcctc gtggtgaagc attttcaggc
      421 ctatctgtac tttgataaga tcgtgtagac ggagaactac ttggagtaga tgttctagtt
      481 cgcgaattat catacgttga cccatctttt gacgatcccg aataagaatt tgaaggacgt
      541 gattctgctt gacttcttcg tgaactataa ctggaacttg gtccatatga atcatatctt
      601 ggttttgatg aataatatcg aggatatgaa taataatatg gataatcata atatctataa
      661 tatgaatatg gataaccgta accataccca taataatatc cggaataata tggtcgataa
      721 tatctacatc cccatcccca atatggtcga taacttatac tccaatatgg gtctcctata
      781 tttattgaaa aagaccaatc aggataccac caataattgt cataataata tgaaggagta
      841 tatgtatcaa aaaaacgacg agaatagtaa taatcatcac tatacacatt tccataataa
      901 ttatttacta tagttggttc cttatcatat gaattatctt gattttgata atcataattt
      961 tgttgctctt cttgcttttg tacaaaatta tcattataca cttgtggatc aagagtgcca
     1021 tctacatata caggttttgt ttgattagta tttgatggtg tagatgtaac ttcatactgt
     1081 tgtcttggag aatacacatc atcttggtac tgagcattta atgcctccca agaactgcat
     1141 gaaactaaag ttatcatgct tactatactt attgaaaaaa ttgttttcat tgttatgtcc
     1201 tccatatttt aaaaatataa atattttttt atttatttgc ataaaaatta cgttttttta
     1261 cgatttcaat tttcatacca aaaatattta tatggcaaaa gaattaacaa aaagaagtga
     1321 aaactattct caatggtaca atgatttagt agtaaaagca gatttagctg agaattcagc
     1381 tgttagagga tgtatggtaa taaaaccata tggatatgct atttgggaaa aaatgcaagc
     1441 tcaattagat tcaatgttta aagcaaccgg acatgtaaat gcatattttc cattatttat
     1501 tccaaaatct ttctttagta aagaagctag tcatgttgaa ggatttgcaa aagaatgtgc
     1561 agttgtaaca cactatagac ttaaaaatga ccctgaaggt aaaggtataa ttgtagacga
     1621 aaatgcaaaa ttagaagaag agcttattgt tcgccccaca tcagaaacca taatttggaa
     1681 tacatacaaa aattggatac aatcatatag agatttaccc atattatgta atcaatgggc
     1741 caatgttgta cgttgggaaa tgagaacacg attattttta agaacagcag aattcttatg
     1801 gcaagaagga catactgctc acgctacaaa acaagaagca atggaagaag cagaaaagat
     1861 gattcgagta tatgctgatt ttgccgaaaa cttcttagct cttcctgtga ttatgggtgc
     1921 aaaaacggct aacgaacgat ttgccggagc tttagaaaca tataccattg aagctctcat
     1981 gcaagatggt aaagcattac aatcaggcac atcacacttt ttaggtcaaa attttgcaaa
     2041 agcttttgat gttacattta caactaaaga aggtacttta gattatgttt gggctacatc
     2101 atggggagtt tctactcgat taatgggagc tcttataatg gctcattctg atgataatgg
     2161 attggtatta ccaccaaaac ttgctcctat tcaagttgta atagttccaa tatacaaagg
//
LOCUS       DAOTOW010000379         2220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38238_length_2220_cov_2.080831, whole genome shotgun sequence.
ACCESSION   DAOTOW010000379 DAOTOW010000000
VERSION     DAOTOW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38238_length_2220_cov_2.080831"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2220)
                     /locus_tag="P5243_07200"
     CDS             complement(<1..>2220)
                     /locus_tag="P5243_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19273.1"
                     /translation="PTVVIGTNKTLPIVATVNKQYIVRVSSDPFAPASAKHTVVVNTL
                     PQLAIQGSNVKQLCLNSSTVITPQVQGNAPFTYAWTDGSPFVITTPNYTYNNSQTPGV
                     QVLNLQVTDANGCKENTNVTITVYDLPKVAIVPPNPSVCVNSELTLSANVTGNGKAPY
                     TYVWTESTGKLTPMGQTATFKSSVVGPFQVSVAVTDDNTCSASASQIVLVKPLATLKL
                     NDEYSVCAGSNLVLDVNPTNIPGSYTMNWVGGARNRIIDSTNVLKSIFKSNDVGNYDL
                     YYAIADINGCPRVDTVTVHVLPAVKLTPITNKDACVGVSLPIAASLISAGNPANVQYA
                     WIGNVIPTFGNQTTFTAGTAGVSNIKLVATEGQGANSCSDSLSFTVTVHSNPRVQITA
                     NVQPNNVPYTSPVQLDGNISFFTTAPYSYQWENAGAVASGQGTSRISTIPITQTRSYN
                     VTVTDAFGCKGSDTIVLSTKNIIIDIWHPCTDAIPISPDVLVSGICLDKKNEICMGQN
                     IRLVPQFMSGDTAGLSYTWTDDEGKVVANTINAVVRPTKQTTIYTLTVANSVGFETKA
                     QFLVLANPNPTANITVSPEYGGSFYIQDPLTINGNPSGGSGDWKKHTWVTSLSLLNTN
                     AQITQVTVPTPQNVSFVYSVEDSKGCKATTSRTITVIAPILPNIIGNDACEKSSTVYS
                     LDRTYPIGTVFVWSVEGGQIVGSDTNPTVEVYWPNARVGKVSVYVKPQYEKEATRDLS
                     "
BASE COUNT          691 a          441 c          384 g          704 t
ORIGIN      
        1 cgataagtcg cgcgttgctt ctttttcgta ttgaggtttc acatataccg aaactttacc
       61 tacacgagca tttggccaat atacttcaac tgttggattt gtatcggaac ctacaatttg
      121 acctccttct accgaccata caaatacagt tcctatagga tacgttctat ctaaagaata
      181 tacagttgaa gatttctcac aagcatcatt accaattata tttggcaata taggtgcaat
      241 tacggttata gttcttgacg ttgtagcctt acacccctta ctgtcttcta cagaatacac
      301 aaaacttaca ttctgaggtg taggtacggt aacttgagta atttgagcat ttgtattcaa
      361 caatgataaa ctagttaccc atgtatgttt tttccaatct cccgaaccgc cacttggatt
      421 accattaata gtaagaggat cttgtatata gaaactacca ccatattcag gcgaaacagt
      481 aatatttgca gttggattag gatttgcgag cactaaaaac tgtgcttttg tctcaaatcc
      541 aacagaattt gcaacagtaa gggtataaat tgtagtttgt ttagttggtc gcacaactgc
      601 attaatagta tttgctacaa cttttccctc atcgtcagtc catgtatatg acaaccctgc
      661 agtatcacct gacataaact gaggcaccaa acgaatattt tgtcccatac aaatttcatt
      721 tttcttatct aaacaaatac ctgaaaccaa tacatcggga cttataggta tagcatcggt
      781 acatggatgc caaatatcaa ttataatatt tttagttgat aatacaatag tatcggaacc
      841 tttacaacca aatgcatcgg taactgttac attatacgaa cgtgtttgag taataggaat
      901 tgtagaaatt cgtgacgttc cttggcccga agcaacagct cccgcatttt cccactgata
      961 cgaatatggt gcagtggtaa agaacgaaat atttccatct aactgcacag gcgatgtata
     1021 tggaacatta tttggctgca cattggcagt tatttgcaca cgtggattac tgtgaactgt
     1081 aacagtaaat gaaaggctat cgctacaact gtttgcacct tgtccttcgg tagcaaccaa
     1141 tttaatatta gaaactccgg cggttccggc agtaaatgtt gtttgattgc cgaacgtagg
     1201 tattacattt ccaatccatg catattgcac atttgcagga ttacctgcac ttattaaact
     1261 agcagcaatt ggcaacgata ctcccacaca cgcatcttta ttggtaatag gtgtaagttt
     1321 tacggcgggt aacacatgta ctgtaactgt atctacacgc ggacaaccat ttatatcggc
     1381 aatagcatag tataaatcat aattaccaac atcattactt ttgaaaatag atttcaatac
     1441 attagtagaa tctattatac gattgcgagc tccaccaacc caattcatag tatatgaacc
     1501 gggaatattt gtaggattta catctaacac taaattagaa cctgcacata cagaatactc
     1561 atcatttagt tttaatgtag caagtggttt tactaatact atttgacttg cacttgcact
     1621 acaggtatta tcatcggtta ccgcaacact cacttggaac ggaccaacca cagaactttt
     1681 gaaagtagca gtttgcccca ttggagttaa tttaccggta gattccgtcc acacataggt
     1741 atatggagct tttccatttc ctgttacatt tgcagatagc gttaattcac tatttacaca
     1801 taccgatgga tttggaggaa caatagcaac ttttggtaaa tcatatacag taattgtaac
     1861 atttgtattt tccttacaac catttgcatc ggttacctgt aaattaagta cttgcacacc
     1921 gggagtttgc gaattattat aagtataatt tggtgtagtt atcacaaacg gactaccatc
     1981 tgtccacgca tatgtaaatg gagcattacc ctgaacttgc ggagtaataa cggtagaaga
     2041 atttaaacac aattgtttca catttgaacc ctgtatagca agctgtggta atgtattaac
     2101 aacaacagta tgttttgcac ttgccggagc aaacggatca ctgcttacgc gaactatata
     2161 ttgtttatta actgtagcaa ctataggtaa tgttttatta gttccaatta ctactgttgg
//
LOCUS       DAOTOW010000380         2209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38481_length_2209_cov_3.448932, whole genome shotgun sequence.
ACCESSION   DAOTOW010000380 DAOTOW010000000
VERSION     DAOTOW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38481_length_2209_cov_3.448932"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="P5243_07205"
     CDS             <1..675
                     /locus_tag="P5243_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HRS19274.1"
                     /translation="PNQADSDRISNYIGVNCKELQEQFPEGNHTETLFSLGELPPKTK
                     IIVKENAEQNSYPVYIRKLAILPMPESLRFVDRINVRRKEQIHGLRMHRMFEYIITLQ
                     DIETAVQQALYEKIIDKTEVSLYEDYCTNAILQSNVQRWFNPQYTIVTEQSILTPQGE
                     KRPDRIVFTSDRDVEVIDYKFTAEQNPKHIVQVAEYVSVLESMGYSVQGFVWYVLQNN
                     VVLVGK"
     gene            complement(717..1388)
                     /locus_tag="P5243_07210"
     CDS             complement(717..1388)
                     /locus_tag="P5243_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19275.1"
                     /translation="MNVKINFFVSVLLLFAISCKEDSAIKHVTYTIDQSTKEWYIADS
                     ITLFTMIDSKGIESSFNQISNYVDYSPGKTIIMGIPTKASKTEQKYQSYSSTYGIPFS
                     VSFYPHSDGYGDHIYINIKEYSCSFDLIHQKVTTVYTPFGSVSLSMTDKGYKNLDKVS
                     STGEILSEYTVQNKTYTSVLHITLLDCIPNTEPYAIREILYAQKYGLIEFVYANGEKQ
                     ERIFD"
     gene            complement(1372..1929)
                     /locus_tag="P5243_07215"
     CDS             complement(1372..1929)
                     /locus_tag="P5243_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19276.1"
                     /translation="MKKYLAIIAICTLFYNFSDCGRCDSKTNYDSGTISEKSLQFVPY
                     KENDTIRFVHSKGSIISFTVARTHAKHADYDRCATFIIDEDKTILTPNYPIFSPVFTI
                     TQLDSLRISYDMYIQQTYFTINPELYIDSISLHTGTYYNVYAIQGYREFPSSKTIKPD
                     SLYYNTTYGILRITMSNGEWYERKN"
     gene            complement(1967..>2209)
                     /locus_tag="P5243_07220"
     CDS             complement(1967..>2209)
                     /locus_tag="P5243_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19277.1"
                     /translation="NELIVKKHDILFQKIKDSIRYFFPWRIVLQRMIQADWMHYAYIT
                     KDMQLFIQQKTGKSFTHFKTFNAIICKWYSLIITNK"
BASE COUNT          765 a          297 c          355 g          792 t
ORIGIN      
        1 ccaaatcaag cagattcaga tagaatatca aattatatag gtgtgaattg taaagaattg
       61 caagaacaat ttcccgaagg gaatcatact gaaacattgt tttctttggg agaattacct
      121 ccaaaaacta aaattattgt aaaggaaaat gctgaacaaa attcttatcc ggtttatatc
      181 cggaaacttg caatattacc tatgccggaa tcattacgtt ttgtagatag aatcaatgtt
      241 cgtagaaaag aacaaattca tggattaaga atgcatagaa tgtttgaata tattattact
      301 cttcaagata ttgaaacagc ggttcaacag gcactttacg aaaaaattat tgataaaaca
      361 gaagtgtctt tatatgaaga ctattgtaca aatgctattt tacagagcaa tgtgcaacgt
      421 tggtttaatc cgcaatatac aattgtaaca gagcaaagta ttcttactcc tcaaggtgaa
      481 aaacgccctg atagaattgt ttttacatct gacagagatg tagaagtaat tgattataaa
      541 tttacggcag aacaaaatcc taagcatatt gttcaggtag cagaatatgt ttctgtatta
      601 gaatcaatgg ggtattcggt tcaaggtttt gtatggtatg tattgcaaaa caatgttgtt
      661 cttgttggta aataacgtcg aattcacgtt aaaaaacaca tacaaataca caagtattaa
      721 tcgaaaattc gttcttgttt ttctccgttg gcatatacaa attctatgag accatatttt
      781 tgagcatata gaatttctct aattgcatat ggttctgtat ttggaataca gtcgagtaat
      841 gtaatgtgta aaacagatgt atatgtttta ttttgcactg tatattcaga taatatttca
      901 cctgttgatg atactttgtc tagattttta taccctttgt ctgtcatgga aagagaaacg
      961 gaaccaaaag gggtatatac agttgtaact ttttgatgaa tcaaatcaaa actgcaagaa
     1021 tattctttta tatttatata aatgtgatct ccgtagccat cactatgagg ataaaatgat
     1081 acagaaaacg gtattccata tgtagatgaa tatgattgat atttttgttc tgttttactt
     1141 gctttggtgg gtatgcccat tattatagtt ttaccgggag aataatcaac atagtttgaa
     1201 atttgattaa aactactttc aataccttta ctgtcaatca tagtaaacag tgttatgcta
     1261 tctgctatat accattcttt ggttgactgg tcaatggtat aagttacatg tttaatagca
     1321 ctatcttctt tacagctaat tgcaaagagt aatagcacac ttacaaaaaa attaattttt
     1381 acgttcatac cattctccat ttgacattgt aattcgtaaa attccgtacg ttgtgttata
     1441 atataaacta tcaggtttaa ttgttttaga acttggaaat tctctatatc cttgtattgc
     1501 atatacatta taatatgttc cggtatgtaa tgatatagaa tcgatatata actcaggatt
     1561 tattgtaaaa tatgtttgtt gaatatacat atcatatgaa atgcgtaatg aatccaattg
     1621 tgttatagta aaaacaggtg aaaatattgg ataatttggt gttaaaatag ttttgtcttc
     1681 atcaataata aatgttgcac acctgtcata atctgcatgt tttgcatggg tgcgtgcaac
     1741 ggtaaaagaa ataatgcttc ctttagaatg aacaaatctt atagtatcat tctccttata
     1801 aggaacaaat tgaagtgatt tttctgaaat tgtacctgaa tcatagtttg tttttgaatc
     1861 gcatcggcca cagtcactaa aattataaaa taatgtacat atagctatta tagcaagata
     1921 ctttttcatg gggttttgat tattgtaaat agttaagcaa atataattac ttatttgtaa
     1981 taattaagct ataccattta catataattg cattaaacgt tttaaaatgt gtaaacgatt
     2041 ttccggtttt ctgttgtatg aaaagttgca tatctttagt tatataagca taatgcatcc
     2101 aatcggcttg aatcattcgt tgtaaaacta tgcgccatgg aaaaaaataa cgaatagaat
     2161 cttttatttt ttgaaaaagt atatcatgtt tttttacaat taattcatt
//
LOCUS       DAOTOW010000381         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38641_length_2202_cov_3.191896, whole genome shotgun sequence.
ACCESSION   DAOTOW010000381 DAOTOW010000000
VERSION     DAOTOW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38641_length_2202_cov_3.191896"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..259
                     /locus_tag="P5243_07225"
     CDS             92..259
                     /locus_tag="P5243_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS19278.1"
                     /translation="MAHKITEDCIACGACAGECPVEAISEGDIYKIDADKCTDCGACA
                     DVCPTGAILAN"
     gene            complement(379..1632)
                     /locus_tag="P5243_07230"
     CDS             complement(379..1632)
                     /locus_tag="P5243_07230"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRS19279.1"
                     /translation="MKADILLGLQWGDEGKGKIVDVLTPKYDIIARFQGGPNAGHTLV
                     FNNIKHVLHTIPSGIFRENAMNVIGNGVVIDPYIFKEEIDKLVAQGIDIHSKIIISSK
                     AHIIMPSHKALDEAYENDKGDKKIGSTKKGIGPTYTDKIARTGLRVGDILLDFNSIYQ
                     SRKQQHIKILSHYSYSFDIEAYEQKWFEGIALIKSMQIVDTEYYINKQLQSGKSVLCE
                     GAQGTLLDIEFGSYPFVTSSNTISAGACVGLGISPKVIGEVFGIFKAYCTRVGSGPFP
                     TELFDETGALMQKYGNEFGSTTGRPRRCGWLDLVALKYAIMLNGVTQLFMMKIDVMNT
                     FDTIKICTAYSYKGSIIDYMPNDVEGVEPIYKEFKGWNCELPSNSFNDLPQEIKTYIE
                     FIEKETGVPVSIISTGPEREKTIIR"
     gene            complement(1714..>2202)
                     /locus_tag="P5243_07235"
     CDS             complement(1714..>2202)
                     /locus_tag="P5243_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS19280.1"
                     /translation="LQGKTTINYLYAAGEVSSSGLHGANRLASNSLLEAIAYADFAAQ
                     TAIAEKDKNPIIHAIPDWNDEGTTLPEEMVLITQNLKEVQQILSNYVGIVRSEVRLKR
                     AYNRLWTIYSETEDLYKQSTLSPKLCELRNLIQVGYLVIKSAKARKKSIGLHYMVSYD
                     VN"
BASE COUNT          721 a          361 c          327 g          793 t
ORIGIN      
        1 aaatataaag aaaaatagga ggtttttcgt ttggtttatt acaaaaaact cctttatatt
       61 tgcaatatag ttaactaaat ttatacatat tatggctcac aaaattacag aagattgtat
      121 tgcatgcggt gcatgtgcag gtgaatgtcc ggtagaagct atttctgaag gagatattta
      181 caaaattgat gctgacaaat gtactgattg cggtgcatgt gcagatgttt gtccaaccgg
      241 tgcaatttta gcaaattaat cttcgcacac aattaatcac aatagtgatt aacaaaaaaa
      301 agagactgat tttatcagtc tctttttttt atatgtatac tttatataaa ctgttttaat
      361 ttgttgcaat taaatatttt atcgaattat tgttttttct cgttcaggac ctgttgatat
      421 gattgaaaca ggtacgcctg tctctttttc aataaattca atataggttt ttatttcttg
      481 aggtaaatca ttaaaagaat ttgaaggtaa ttcacaattc catcctttaa attctttata
      541 aataggttca acaccttcta catcatttgg catataatct attatcgaac ctttatatga
      601 atatgctgta catattttaa ttgtgtcaaa tgtattcatt acatcaattt tcatcataaa
      661 taattgagta acaccattaa gcattatagc atattttaat gctacaaggt ccaaccagcc
      721 gcatcgacga ggacgtccgg tagtagagcc aaattcatta ccgtattttt gcattaatgc
      781 tccggtttca tcaaataatt cggtaggaaa aggaccgctt ccaactcgag tgcagtatgc
      841 tttaaaaata ccaaatactt cacctattac tttaggtgaa atacctaaac caacacatgc
      901 accggcagaa atagtattag aagaggtaac aaaaggatac gagccaaatt ctatatcaag
      961 caatgtccct tgagctcctt cacataaaac agattttcct gattgtagtt gtttgttaat
     1021 ataatattct gtatcaacta tttgcattga tttaattaaa gctatacctt caaaccactt
     1081 ttgctcgtat gcttcaatat caaatgaata tgaataatgt gacaaaattt taatatgttg
     1141 ttgtttgcgt gattgataaa tagaattgaa atcaagtaaa atatcaccaa cgcgtaatcc
     1201 ggttcgtgct attttgtctg tataggtagg accgatacct ttttttgtag aacctatttt
     1261 tttatcacct ttgtcatttt catatgcctc atctaatgct ttatgtgaag gcattataat
     1321 atgagcttta gaagatataa ttattttaga atgaatatca ataccttgtg caacaagctt
     1381 atcaatttct tctttaaaaa tatatgggtc aattacaaca ccattaccaa ttacgttcat
     1441 ggcattttct ctgaaaatac ctgaaggaat tgtatgcaat acatgtttta tattattgaa
     1501 aaccaatgta tgaccggcat tgggaccacc ttgaaacctt gcaattatgt catattttgg
     1561 agttaaaacg tctacaattt tccctttacc ttcgtcgccc cattgaagcc ctaataaaat
     1621 atctgctttc atgtattatt gtttgttatt actattaaaa aaaccgagta aaagtacaaa
     1681 aaaaatgaaa aaatgtaaga atacttttca tatttaatta acatcatatg aaaccatata
     1741 atgtaatcca atactttttt tgcgagcttt tgcacttttt ataactaaat agcctacttg
     1801 aataagatta cgtaattcac acaatttggg tgagagggta gattgtttgt ataaatcttc
     1861 tgtttcactg taaatagtcc ataatctgtt gtatgcacgt tttaatcgta cttcactacg
     1921 aactatacct acataattac ttaaaatttg ttgtacttct tttaaatttt gagttataag
     1981 caccatttct tcaggtagag tagtgccttc atcattccag tcgggtattg catgtataat
     2041 aggattttta tctttttctg ctattgctgt ttgtgccgca aaatcagcat aggcaattgc
     2101 ttcaagcaaa ctattagaag caagcctatt tgcaccatgc aatcctgatg atgaaacttc
     2161 acctgcggca tataaataat ttatagtagt tttgccttgt aa
//
LOCUS       DAOTOW010000382         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38643_length_2202_cov_2.822077, whole genome shotgun sequence.
ACCESSION   DAOTOW010000382 DAOTOW010000000
VERSION     DAOTOW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38643_length_2202_cov_2.822077"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="P5243_07240"
     CDS             complement(<1..436)
                     /locus_tag="P5243_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19281.1"
                     /translation="MSKFYIQQGELQKSDALNIQGFEAFTNTLYVYEQVRLYNTVPLF
                     LEEHMQRIITTLDICKIPIPQQFHKDTIQRYITRLLNVNKVYKGGVCTILITPSINKS
                     NWECLLFIEPIEVLEFVFNSGGIRIGIFSDCIQRRFRQTLIFN"
     gene            complement(429..920)
                     /locus_tag="P5243_07245"
     CDS             complement(429..920)
                     /locus_tag="P5243_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRS19282.1"
                     /translation="MNKAVFLDRDGVINDGTQYYTYSVDSFIFNPGIFDGLRLLQQHG
                     FMLIVITNQSGVAKGVYSIADVERVHTYMVSELERHDITITQVYYCPHHPDVSACECR
                     KPGTLLFEQAIKTYNIDVHNSYMIGDSKRDIEAAYKVGVKGILISKNESIVPYCKAII
                     GNE"
     gene            complement(932..1711)
                     /locus_tag="P5243_07250"
     CDS             complement(932..1711)
                     /locus_tag="P5243_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS19283.1"
                     /translation="MNESYPMLIRFLGTGTSQGIPVIACNCEVCKSTSMYDKRLRSSV
                     LITINDITIVIDAGPDFRQQMLAGDIQNIDAILITHEHKDHIAGLDDIRPYNFLKQKA
                     IDVFAEARVIDAIKQEFSYVFAKEKYPGIPIMNIHTIENKPFEYKSIQIEPIRVMHWN
                     LPIFGYRIGKVAYISDASYIDAQEIKKLTNLDVLIINALRIKKHYSHFNLDEALHIVE
                     QLQPKQAFFTHISHLMGFHEQVQARLPRQVYLSYDGLTISI"
     gene            complement(1718..>2202)
                     /locus_tag="P5243_07255"
     CDS             complement(1718..>2202)
                     /locus_tag="P5243_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS19284.1"
                     /translation="FVGNPHDEISPELSAKIIIEHVVYGRELARKHNLPGIVIEMIFG
                     HHSNSKTWFFYDKFKKLNPNQQIDDQMFQYPASKPTRKELAVLMIADSLEAASRSIVI
                     HDKEQISLMVDKIIQNLMSESHFDNVDITLRELSTVKDVMIQKLINMYHARIPYPDKK
                     "
BASE COUNT          761 a          380 c          297 g          764 t
ORIGIN      
        1 gattaaatat gagagtctgc ctaaaccttc tttgtatgca atcagaaaaa atccctatac
       61 gtatacctcc cgaattaaac acaaattcta aaacttctat tggctcaata aaaagtaaac
      121 attcccaatt cgatttatta attgaaggag ttataagaat tgtacacacg cctcctttat
      181 acaccttatt tacattaagt aatcgtgtaa tgtaacgttg aattgtatct ttatgaaatt
      241 gttgcggaat tggaatttta catatatcta atgttgtaat aatacgttgc atatgttctt
      301 ctaaaaacaa aggaacggta ttatataaac gcacttgttc atatacatac aaagtgtttg
      361 taaatgcttc aaacccttga atattcaatg catcactttt ttgcagttct ccttgttgaa
      421 tataaaattt actcattgcc aattatagct ttgcaatatg gaactatgga ttcatttttc
      481 gatattaata ttcctttaac acctactttg taagcagctt caatatctct tttactatca
      541 ccaatcatat atgaattatg aacatcaata ttataggttt taatagcttg ttcaaataac
      601 aatgtaccgg gctttctaca ttcacaagca gaaacatcag gatgatgcgg acaataatat
      661 acctgagtaa ttgtaatgtc atgtctttct agttcactaa ccatatatgt atgtactcgt
      721 tcaacatcgg ctatagaata tacacctttg gcaacaccac tttgattagt tataactata
      781 agcataaaac catgctgttg caacaaccta agcccatcaa aaattcccgg attaaatata
      841 aatgaatcaa cactatacgt ataatattga gtaccatcat taattacccc atctctatct
      901 aaaaataccg ccttattcat actattgcca tttatataga aattgttaat ccatcatacg
      961 ataaatatac ctgccgaggt aaccgagcct gaacttgttc atgaaatccc ataagatgac
     1021 taatatgagt aaaaaatgct tgtttgggtt gtaactgttc aactatatgc aaagcttcat
     1081 ctaaattaaa atgcgaataa tgttttttga ttcgcaatgc attaattata agaacatcta
     1141 aatttgtaag ttttttaatt tcttgtgcat caatatatga tgcatcagat atataagcaa
     1201 ctttaccaat tctatatcca aatataggca aattccaatg cattacccgt attggctcta
     1261 tttgaattga tttatattca aatggtttat tttcaatagt atgaatattc attataggaa
     1321 tacccggata tttttctttg gcaaacacat atgaaaactc ttgtttaatg gcatcaatta
     1381 cacgtgcttc agcaaaaaca tcaatagctt tttgttttaa aaaattataa ggacgaatat
     1441 catctaatcc tgctatatgg tctttgtgtt catgagtaat aagaatagca tcaatattct
     1501 gaatatcacc tgcaagcatt tgctgtctaa aatcgggtcc ggcatctata actatagtaa
     1561 tatcattaat agtgataaga acagaactac gcaagcgttt gtcatacata cttgttgatt
     1621 tacatacctc acaattgcat gctattacag gtattccttg agaagtaccg gttcctaaaa
     1681 aacgtataag cataggatac gattcattca taatgcatta ctttttatca gggtagggaa
     1741 tacgagcatg atacatattt ataagtttct gaatcattac atcttttact gttgacaatt
     1801 cacgcaatgt aatatctaca ttgtcaaaat gactttcact cataagattt tgaataattt
     1861 tatcaaccat aagcgaaatt tgttctttat catgaattac aatacttcga gatgcagctt
     1921 ctaaagaatc tgcaatcata agaacagcta attctttacg agttggttta gatgccggat
     1981 attgaaacat ctgatcatca atttgttgat taggatttaa ttttttgaat ttatcgtaaa
     2041 aaaaccaagt tttagaattg ctatgatgtc caaatatcat ttcaataaca ataccgggta
     2101 aattatgttt acgagccaat tctctaccat acaccacatg ttcaataata attttagcac
     2161 ttaactcagg agatatttcg tcatgaggat ttccaacaaa tg
//
LOCUS       DAOTOW010000383         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38852_length_2193_cov_3.553321, whole genome shotgun sequence.
ACCESSION   DAOTOW010000383 DAOTOW010000000
VERSION     DAOTOW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38852_length_2193_cov_3.553321"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1543)
                     /locus_tag="P5243_07260"
     CDS             complement(125..1543)
                     /locus_tag="P5243_07260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRS19285.1"
                     /translation="MLFNSSVFAIFLCIVFCLYWFVFKQIKQQNILLLLASLFFYGWW
                     DWRFLSLICFSSVLDFAIGLALQNTQIQSKRKWLLITSIVVNIGLLFTFKYYNFFVES
                     FIYVFHTFGINLNIQTLSIILPLGISFYTFQTLSYTIDVYKRVFEPTKDIIAFMAYIS
                     FFPQLVAGPIERASNLLPQFFRKRTFDKTFALEGLKIMLWGFFKKIVIADGISVLIDP
                     IFKSPEYYSGGTLAIAGVLFAVQIYADFSGYSEIAIGTARLFGFRLMKNFNYPYFASS
                     IIDFWKRWHISLTSWFKDYIYIPLGGNRHGLRKQIINTCIIFIVSGIWHGANFTFILW
                     GVAHAIFFIPGILFRKKIYAIPAYANSILIIGKILYTNCIVILLWFLFRAQSIEDVVI
                     IFSKIFSADIFELNTTFPFHIFVLVCILMITEWFGRRNDYAIVGIAGNAPRMVRWSIY
                     YGIMITILYYFETPLNYIYFQF"
     gene            complement(1533..>2193)
                     /locus_tag="P5243_07265"
     CDS             complement(1533..>2193)
                     /locus_tag="P5243_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS19286.1"
                     /translation="IEPIVQGAGGLYFYHPTYLKELAALCKTYDILVIADEIATGFGR
                     TGKLFACEHAAVEPDIMCIGKAITGGYMSFAATLCSHTIAHSISSASPGVFMHGPTFM
                     GNPLACAIACESIDLLLEFNWQQTILAIESQLTKLLAPLQNHSSVADVRVLGAIGVVE
                     MKKPVDMKTIQPLFVDHGVWVRPFGKLIYVMPPYCITSDELVRLTAAITTIVTEYSHV
                     I"
BASE COUNT          807 a          408 c          335 g          643 t
ORIGIN      
        1 acagcatatt ctcctgcact gtacgttttt gattttcgta attgatgtga tatccatata
       61 tctataccaa cgctcagtat tacaaatagt gcaataaaca aaataatatg ttgaataaat
      121 cctttcaaaa ctgaaaataa atataattta agggagtttc aaaataatac aaaattgtta
      181 tcataatacc ataatatata ctccaacgaa ccattcgagg agcatttccg gctataccaa
      241 caattgcata atcatttcgc cttccaaacc attctgtaat cattaaaata catacaagaa
      301 caaagatgtg aaaaggaaaa gttgtattga gttcaaatat atcagcactg aaaattttcg
      361 aaaaaattat cactacatct tcaattgact gggctctgaa taaaaaccaa agcaatataa
      421 caatacaatt ggtgtacaat attttaccaa taataagaat actattggca tacgcaggta
      481 tcgcatatat tttttttcga aaaagaattc cgggaataaa aaatatagca tgcgcaactc
      541 cccacagaat aaatgtaaaa tttgctccat gccaaatacc gcttacaata aaaataatac
      601 aggtattgat aatttgtttt cgcaacccat gcctattgcc tcccaatgga atgtagatat
      661 agtctttgaa ccaactggtt aacgatatat gccagcgttt ccaaaaatca ataatcgaag
      721 aggcaaaata tggataatta aaatttttca tcaatcgaaa cccaaacaat cgcgctgtac
      781 caattgcaat ttctgaatat cctgaaaaat cggcatatat ttgaacagca aacagtaccc
      841 ctgctatagc caatgtgccg ccactataat actccggaga tttaaatata gggtcgataa
      901 ggacagaaat tccgtcggct atcacaattt ttttaaaaaa tccccaaagc attattttta
      961 atccttctaa tgcgaatgtt ttatcaaatg tacgttttct aaaaaattgc ggtaacaaat
     1021 tcgatgctcg ttctatgggc cctgcaacca attgcggaaa aaatgagata tatgccataa
     1081 aagcaataat gtcttttgta ggttcaaaaa cacgtttata cacatcaata gtataactta
     1141 aggtttgaaa cgtatagaaa ctaatgccta atgggagtat aattgaaagt gtttgaatgt
     1201 tgaggtttat tccaaacgta tgaaatacat atataaacga ttctacaaaa aaattatagt
     1261 atttaaaggt aaataataaa ccaatattca caactatact ggtaataaga agccattttc
     1321 gtttgctctg tatttgagta ttttgcaatg ccaaacctat tgcaaaatct aaaactgaag
     1381 aaaaacaaat aagggataaa aaacgccaat cccaccatcc ataaaaaaat aaacttgcaa
     1441 gtaacagcag tatgttttgt tgttttattt gcttaaaaac aaaccaatac aaacaaaata
     1501 cgatacaaag aaaaatagca aaaaccgatg aattaaataa catgtgagta ttctgttact
     1561 attgttgtaa ttgctgctgt taaacgtact aattcatcag atgtaataca atacggtggc
     1621 attacatata taagtttacc aaacggacgc acccatacac catggtctac aaataatggc
     1681 tgaatagttt tcatatcaac cggttttttc atttcaacta cccctattgc acccaaaacc
     1741 ctaacatcgg ctaccgatga atggttttgc aatggagcaa gtaattttgt aagctgcgat
     1801 tctattgcaa gtattgtttg ttgccaattg aattccaaaa gcaaatcgat actttcgcag
     1861 gctatagcac atgccaaagg atttcccata aatgtagggc cgtgcataaa tacgccggga
     1921 gaagcactcg atatgctgtg tgcaattgta tgactacaca acgtggctgc aaaactcata
     1981 tatccaccgg taatagcttt gcctatacac attatatcgg gttcaacggc tgcatgttcg
     2041 catgcaaaga gttttcctgt acggccaaat ccggttgcaa tttcatcggc tattacaagt
     2101 atatcgtacg ttttgcacaa tgctgccaat tctttcaaat aagtaggatg atagaagtac
     2161 aggccaccag ctccttgcac aataggttct ata
//
LOCUS       DAOTOW010000384         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38910_length_2191_cov_3.021067, whole genome shotgun sequence.
ACCESSION   DAOTOW010000384 DAOTOW010000000
VERSION     DAOTOW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38910_length_2191_cov_3.021067"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..910)
                     /locus_tag="P5243_07270"
     CDS             complement(161..910)
                     /locus_tag="P5243_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19287.1"
                     /translation="MRLLYVVILVFILSVTAHAQQMLYGKIVNSQTFTSLSGVYVENT
                     TKHTLAESDTSGVFTISVDYGDTLLFSSIGYHWKKYVVVDGNHQTFLLDEQIYDLSKV
                     VKHAPLDYESFKYAILSMKFPKDSLHVNLVYEKYIPIKEYTPGQIGVASIDGAITGLY
                     NSFNKHAKNQLRAVELLEQKHIIVIANKKFTKELVLDVTRLPKEYLNEFIAFCAFTDE
                     FIASASEYQIILALQYKTILFLQRYPHIQKM"
     gene            complement(1013..>2191)
                     /locus_tag="P5243_07275"
     CDS             complement(1013..>2191)
                     /locus_tag="P5243_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19288.1"
                     /translation="GWGTQNSIVVNQAGTTHLQVRDSYGCVTSDDFTITEVYALPTVS
                     LGDDKQVCQGSSTTLSPTVTGNAPFTYTWNTNANSQTLSVNADGNYSVEVTDKNGCKA
                     SDEVAVAILRPHKEEIGVATFAKDGKGVVIAWERTLGKRTASYEVMRETGESGTYISV
                     GTLPFNAPESFIEDKDADVLTKSFTYKLITRDSTCNNTAESAPHTTVVVAYNVSQITG
                     KATLQWSKYVGLPIATYRIKKGTNVNNLVQVDSVSGSAAPMWNDPVPYQFGTIYRVEM
                     KLPDTVETRWEMLKIESGPFTLALSNIAEAQTSIQQVSDVSMKVYPTIATSQVSIDFD
                     AEMNNVTIQLVSSSGTVVYSSVVSGNSFTIPLSGVAKGVYSVKVIVNSQVQTIPIVVQ
                     "
BASE COUNT          768 a          389 c          304 g          730 t
ORIGIN      
        1 aatctcaaat cattttacta tatttacaac ttgtcaaaaa agacagccat ttacccatat
       61 atattgttaa atcctaaaaa catttcatat atggaatttt tggattttat aaaacaatca
      121 caatctaaca aataaaaata attgtatatt caataagaaa ttacattttt tgaatatgcg
      181 gatacctctg taaaaacaaa atagttttat actgcaatgc caaaattatt tgatattccg
      241 aagcactagc tataaattca tcagtaaaag cacaaaatgc tataaattca ttcaaatatt
      301 cttttggtaa gcgagtaaca tctaacacta attctttagt aaatttttta tttgcaatta
      361 caataatatg tttttgttca agcaattcaa ctgctcgcaa ttgattttta gcgtgcttat
      421 taaaagaatt atacaaaccg gtaatagcac catcaataga tgcaactcca atttgtcccg
      481 gagtatattc tttgataggt atatactttt catacacaag atttacatga aggctatctt
      541 tgggaaactt cattgataat attgcatatt taaaactttc ataatcaaga ggagcatgct
      601 ttaccacttt actcaaatca taaatttgct catcaagtaa aaatgtttga tgattaccgt
      661 ctactactac atattttttc caatgatacc ctatagaaga aaacaataat gtatctccat
      721 aatcaacaga tattgtaaaa acaccggaag tatcagattc tgctaatgta tgttttgttg
      781 tattttcaac atatacaccc gacaacgatg taaaagtttg agagttaacg attttaccat
      841 acaacatctg ttgagcatgt gctgtaacag acaaaataaa aaccaaaata actacatata
      901 acaatcgcat atctattgtt ttttggtaaa aatacaagca ttttttttga aatgcaagaa
      961 taaaaaaagc tgcctgaaat ggcagctttt ttaaactcat attttgtgtt tattattgca
     1021 caactatagg aatagtttgt acttgagaat tcactattac tttaacagaa tatacccctt
     1081 tagctacacc acttaatgga atagtaaatg aatttcctga aacaacagaa gaataaacaa
     1141 cagttcctga acttgaaact aattgtatgg taacattgtt catttcggca tcaaaatcta
     1201 ttgatacttg agacgttgct attgttggat aaactttcat agatacatct gatacttgtt
     1261 gtattgaagt ttgagcttcg gcaatattac tcaatgcaag agtaaacggt ccactttcaa
     1321 ttttcaacat ttcccaacga gtttctacgg tatcaggtaa tttcatctca actctataaa
     1381 tagtaccaaa ttgatacgga acaggatcgt tccacatagg agcagcagaa cctgaaactg
     1441 aatcaacttg aactaaatta tttacattag tacctttttt gattctataa gtagctatag
     1501 gtaatcctac atatttagac cattgtagag tagctttacc ggtaatttga cttacattgt
     1561 aagcaactac tactgttgta tgaggagcac tttctgcagt attattacaa gtagaatcac
     1621 gtgttattaa tttatacgta aatgatttag tcaatacatc agcatcttta tcttcaataa
     1681 acgattcagg agcattaaaa ggtaaagtac caacagatat atatgttcct gattctccag
     1741 tttcacgcat tacttcgtaa cttgcagtac gtttaccaag agtacgctcc catgctatta
     1801 ctacaccttt tccatcttta gcaaatgtag ccacaccaat ttcttctttg tgaggacgaa
     1861 gaattgcaac ggcaacttca tcagatgctt tacaaccatt tttatcggta acctcaactg
     1921 aataatttcc atcggcattt acagacaatg tttgtgaatt ggcatttgta ttccatgtat
     1981 aagtaaatgg agcatttcct gttactgtag gagaaagcgt cgtagaagat ccttgacaaa
     2041 cttgtttatc atcacctaaa cttacagttg gcaacgcata aacctctgta attgtaaaat
     2101 catcagacgt tacacaacca tacgaatcac gaacttgcaa atgagttgta ccggcttgat
     2161 ttacaacaat agaattttga gttccccaac c
//
LOCUS       DAOTOW010000385         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38917_length_2191_cov_2.618914, whole genome shotgun sequence.
ACCESSION   DAOTOW010000385 DAOTOW010000000
VERSION     DAOTOW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38917_length_2191_cov_2.618914"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..811
                     /locus_tag="P5243_07280"
     CDS             230..811
                     /locus_tag="P5243_07280"
                     /inference="COORDINATES: protein motif:HMM:NF020315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19289.1"
                     /translation="MNCYNYLFQILFLALLQLSVADQFIVIQFDDDKAFENALQDSHS
                     VFCSVGTTQKKVKGNKDNYRKVDYDIPVRAAFFAEKQKCKHFSLVSSVGANSLSSNFY
                     LHLKGETENSVTKYSLPSIAIYRPSMLLGKRKELRPAEEIAKFISCIFSIVIPTSYKP
                     IHAKTIANAMINNAKLSKLGITYYTYTQITKSI"
     gene            891..>2191
                     /locus_tag="P5243_07285"
     CDS             891..>2191
                     /locus_tag="P5243_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS19290.1"
                     /translation="MKKILIILLIFNSLHTYSQKFTISGYVKDASTGEVLIGASVYVL
                     GTTTGAFTNYYGFYSITLEKGNYNIEYSYLGYNTKTILIQLSANKTHNVELSASVEKL
                     ETVVVSAEARDKNVTSVEMGTQSLQMAEIKNIPVLFGENDILKTIQLMPGVKGAGEGN
                     SGFFVRGGGADQNLVLLDEAPVYNASHMLGFFSVFNGDAIRDVKLYKGAMPAEYGGRL
                     SSVLDIKMKEGNLKSYHATGGVGTISSRLAVEGPIKKDTSSFIIAARRTYADMFLLFA
                     KDTNLRKTKLYFYDLNLKTNYTINENNRVFLSGYFGRDVFKFQDMFGTDWGNATGTLR
                     WVHLFNPKLFLNSTIVYSSFDNVITVNNGMYDIDLKMGINDVNLKEDFQWYANTKNTV
                     KFGFESIYHTFNPGELTTDMEGVNISIDNKYALETGLYISN"
BASE COUNT          829 a          312 c          352 g          698 t
ORIGIN      
        1 cctcttaaag ccggcgaaat tgctgaatta tcggggttag acaaaaaaga tgtagaaaaa
       61 gcaatgaaag cattacaaaa aaaagatgct attttttctc ctatccgttg ccaatggcaa
      121 gcaaaataac taaaaaaaaa ctatgtacca tagcttttat gctatggtac caaactgtaa
      181 aatatgaata aaaaagctac cattattgga gcaacaggat taataggtaa tgaactgtta
      241 caattactta tttcagatac tttttttagc tctattacaa ttatcagtcg cagaccaatt
      301 cattgtaata caatttgatg atgataaagc atttgaaaat gcattacagg attcacattc
      361 tgttttttgc agtgttggta ctacacaaaa aaaagtaaaa ggtaacaaag ataattacag
      421 aaaagttgac tatgacatac ctgtacgagc tgcgttcttt gctgaaaaac aaaaatgcaa
      481 acacttttca cttgtttcaa gcgtcggtgc aaatagttta agctctaatt tttatttaca
      541 cctcaaaggt gagacagaaa attctgtcac aaaatattca ttaccttcta ttgctattta
      601 tagaccatct atgttacttg gaaaaagaaa agaattacga cctgccgaag aaattgctaa
      661 attcatttca tgcatatttt ccattgtaat tcctacatcg tataaaccaa ttcatgctaa
      721 gactattgca aatgctatga taaataacgc aaaattatct aaacttggta ttacatatta
      781 tacatatact caaattacta aaagcatatg aattatttta attactcata aggaaacgat
      841 attaaaaaaa aacgtatctt tgaaacaaaa aattttaaat aaaataaaca gtgaaaaaaa
      901 tattaattat actattgatt tttaattcat tacatacata ttcacaaaaa tttacaatta
      961 gtggatatgt aaaagatgca tcaacaggtg aagttttaat aggagcatca gtatatgttt
     1021 taggaacaac aacaggggca ttcacaaatt attatggttt ttattctatt actcttgaaa
     1081 aaggaaatta caatatagaa tattcatatt taggctacaa tacaaaaaca attcttattc
     1141 aactttcagc gaataaaaca cacaatgtag aattaagtgc aagtgttgaa aaacttgaaa
     1201 ctgttgttgt aagtgcagaa gctcgcgaca aaaacgtaac atcggtagaa atgggaacac
     1261 aatcgttaca aatggccgaa atcaaaaata ttcctgtttt atttggtgaa aatgatattt
     1321 tgaaaacaat acaattaatg cccggtgtta aaggtgccgg cgaaggaaac agcggttttt
     1381 ttgtacgagg tggaggtgcc gatcaaaatt tagtattact tgatgaagct cctgtttata
     1441 atgcatctca catgctaggt tttttctctg tttttaatgg tgatgctatt cgcgatgtaa
     1501 agctttacaa aggagcaatg cctgcagaat atggaggcag attatcatcg gttcttgata
     1561 ttaaaatgaa agaaggtaat cttaaatcgt accacgctac gggaggtgtt gggactatct
     1621 catctcgttt agctgttgaa ggacctataa aaaaggatac cagttctttt attattgcag
     1681 ccagacgcac atatgccgat atgtttttac tatttgcaaa agacactaat ttacgaaaaa
     1741 ctaaactcta tttttatgat cttaatttaa aaacaaatta tactataaat gaaaataaca
     1801 gagtatttct ttcgggttat tttggacgcg atgtgtttaa atttcaagat atgtttggta
     1861 ccgattgggg aaatgcaaca ggaacattgc gatgggtaca tttatttaat ccaaaactat
     1921 ttttaaattc tactattgta tatagtagtt ttgataatgt tataactgta aataacggta
     1981 tgtatgacat agatttaaaa atgggaatta atgacgtaaa ccttaaagaa gattttcaat
     2041 ggtatgcaaa taccaaaaac acagtcaaat ttggatttga atctatatat catacattta
     2101 atccgggaga attaacaaca gatatggaag gtgtaaacat ttcaattgat aataaatatg
     2161 ctttagaaac aggattatat attagtaatg a
//
LOCUS       DAOTOW010000386         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_38929_length_2190_cov_3.550820, whole genome shotgun sequence.
ACCESSION   DAOTOW010000386 DAOTOW010000000
VERSION     DAOTOW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38929_length_2190_cov_3.550820"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            514..1530
                     /gene="ruvB"
                     /locus_tag="P5243_07290"
     CDS             514..1530
                     /gene="ruvB"
                     /locus_tag="P5243_07290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRS19291.1"
                     /translation="MNSNLDPEHISNNDKEFERALRPLEFSDFKGQNKVVDNLKVFVQ
                     AAKMRGESMDHVLLHGPPGLGKTTLSNIIANELGVHMKITSGPVLDKPGDLAGLLTNL
                     EENDVLFIDEIHRLSPVVEEYLYSAMEDFRIDIMIDKGPSARSVQLTLNPFTLVGATT
                     RSGLLTSPLRARFGITCLLEYYDAELLTNIIMRSAKILQVPIETKAAHEIASRSRGTP
                     RIANALLRRVRDFAQVKGNGNIDIEITKYSLEALNIDAYGLDEMDNKILCTIIEKFNG
                     GPVGLNTIATAVGEDAGTIEEVYEPFLIKEGFLQRTQRGREVTPLAYKHLGKDRFTKQ
                     GYLF"
     gene            1553..>2190
                     /gene="map"
                     /locus_tag="P5243_07295"
     CDS             1553..>2190
                     /gene="map"
                     /locus_tag="P5243_07295"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRS19292.1"
                     /translation="MKLDPNDPCWCGSGKKYKKCHRTFDMETDKYRREGYIILPQKIL
                     KTAQDIEGIKAAGVITRGILDLMETEVKEGMSTQKIDDLVYEYTKDHGGIPATLNYHG
                     YPKSCCTSINEVVCHGIPSPDRFLKDGDIINIDITTILHGYFADSSRMYCIGDVSAEA
                     KQLVDVSQQCLQVGIEQVKPYTRLNNIGDAIDTYANRFGYSIVRDLCGHGIG"
BASE COUNT          753 a          321 c          410 g          706 t
ORIGIN      
        1 gaaaaaatta ttgatttgat ttgatttgat ttgatttgat tttcatagtt taaaatttta
       61 aatgtttatt aattttttaa ggggatatgt ttatagttgc atatcttata ttatatataa
      121 taagaatatt gtcgcggacc tcctttcttt tcaaaattgt aattttatta ttgattatgt
      181 aaccgtaaaa atataattat ttttttaaat tcaaaaaaat atttaatttt ttaaaaatat
      241 tctttatcat ttagcctgaa ttatatacgc ttaaaagata ttatactgtg tatggtattt
      301 tttctcagtt caattttttg ccttataatg agcaaaaagt tatgttccgt ttgaaacaaa
      361 ctagaaatat aaatttggat tataaataaa gaaaattata tctaaaaaaa agataattaa
      421 attgtcttat acatagggag tattataatt ttttaaacca ttatccttca ttaccacaaa
      481 aaaacctatt tttgtaatta aataattgtg gtaatgaatt caaacttaga tcccgaacat
      541 atttcaaaca acgacaaaga atttgagcga gcgttgcgtc cactcgaatt ctctgatttt
      601 aaaggtcaga acaaggttgt tgataatttg aaggtgtttg tgcaagctgc caaaatgcgt
      661 ggcgaatcta tggatcatgt actattgcat ggacctccgg gattgggaaa aactaccttg
      721 tcgaacatta tagccaacga acttggggta cacatgaaaa ttacctcggg tcctgtgctc
      781 gataaacccg gcgacttagc aggcttactt accaatttag aagaaaacga tgtgttgttt
      841 atcgacgaaa ttcatcgctt atcgcctgtg gtcgaagaat atttgtattc ggctatggaa
      901 gattttagaa tagacataat gatagacaaa ggtccaagtg ctcggtcggt acaacttacc
      961 ctcaatccgt ttactttggt tggtgctact actcgttccg gtttgcttac aagcccttta
     1021 cgagctcgat ttggtattac ttgtttatta gaatattacg atgcggaatt gttgacaaat
     1081 attattatgc gttctgcaaa aattttacaa gtacctatag aaacaaaagc agcacacgaa
     1141 atagcttcgc gtagtcgtgg tacaccacgt attgcaaatg ccttattacg aagagttcgc
     1201 gattttgcac aggtaaaagg taatggtaat atagatattg aaattactaa atattcgctc
     1261 gaagctctta atatcgatgc ctatggctta gacgagatgg ataataaaat tttgtgtact
     1321 attatcgaaa aattcaatgg aggacccgtt ggtctcaata ccatagctac tgccgtaggc
     1381 gaagatgcag gaactattga agaagtgtat gaaccatttt tgataaaaga aggatttttg
     1441 caacgtacac aacgaggaag agaagttacc ccgttagcat ataaacattt aggtaaagat
     1501 agatttacaa aacaggggta tttgttttaa ataaataaaa gagattacag aaatgaaatt
     1561 agatccaaat gatccctgtt ggtgtggcag cggaaaaaaa tataaaaaat gtcatcgtac
     1621 cttcgatatg gaaacagata aatatcgtcg tgaaggatat attattttac cccaaaagat
     1681 attaaaaaca gcacaagata ttgaaggtat taaagctgcg ggtgttatta ctcgtggtat
     1741 attagattta atggaaaccg aagtgaaaga aggtatgtca actcaaaaaa tagatgattt
     1801 ggtgtatgaa tataccaaag atcacggagg aataccagct acactcaatt atcatggata
     1861 tccaaaaagt tgttgtacct ctatcaacga ggtggtatgc catggaatac cgtcacctga
     1921 tagattttta aaagatggtg atatcataaa tattgatatt accacaattt tacatggata
     1981 ttttgctgat agtagccgta tgtattgcat aggcgacgta tctgccgaag caaaacaatt
     2041 agtagacgtg tcgcaacagt gcttgcaagt gggaatagag caagtgaaac cgtatactcg
     2101 actcaataat attggcgatg ctatagatac ttatgcaaac cgttttgggt atagcatagt
     2161 tcgcgattta tgcggtcatg gtataggcaa
//
LOCUS       DAOTOW010000387         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39053_length_2185_cov_2.953521, whole genome shotgun sequence.
ACCESSION   DAOTOW010000387 DAOTOW010000000
VERSION     DAOTOW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39053_length_2185_cov_2.953521"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1351)
                     /locus_tag="P5243_07300"
     CDS             complement(140..1351)
                     /locus_tag="P5243_07300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS19293.1"
                     /translation="MNILQICNKFPYPLKDGGVIAVFTMFKGYYLLQHNVTVAAINTS
                     KHYTNILEIPDSVASMAEFHAVPLQTNVTPWGALYNLFFSSMPYTATRFLHKKFEKEL
                     ITILQTQSFDVIQIEGLYMCGYIPIIRKYSKAIISYRSHNIEYKIWERLLAETHFVPK
                     KWYISNLVSRIKKLEQKTIDTYDVLVPITQFDADIYKDMGNTKPVFVAPTGVFTESLN
                     PVESHEKTISLFHIGGLDWAPNQQGILWFIEKCWPHIYAQFPWLRFTIAGRNASPQFI
                     KKMKVPGVAFVGEVEDSRQFMQENTIMIVPLLAGSGMRIKIIEGLALGKAIVSTSIGA
                     EGIHAVHNESICIADSAQEFIDAVVLLIKKYELRCAIEKNAVTFVSSNFDNATIISKL
                     IEFYKKHGSVD"
     gene            complement(1329..>2185)
                     /locus_tag="P5243_07305"
     CDS             complement(1329..>2185)
                     /locus_tag="P5243_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRS19294.1"
                     /translation="DKQYLILTLYNGKRYEDVVEGLEYNYIRTKRPFREQYFKVQQVL
                     FELESFAFNRTDMELFKHNSQMQNMSQLLTTIDSMKTTLAYRNERSFNHYFTNNIYRS
                     RSKIDKGTDANIDSIYNEFNNAYKIQSFIRALNYARSGEAFLTTAVENEQMQDISLNK
                     HKIELHKKFTLAFACLIMFFVGAPFGAMVRKGGLGVPVIFSFLFYLIYYVLSMIGEKS
                     VKSAESPVFQGMWYPTGIILLIGVYFTYMAIHDKIITPPKDIFIQIYKKIVQFFKQHS
                     KSEHSSNM"
BASE COUNT          751 a          373 c          290 g          771 t
ORIGIN      
        1 tattgataca tatggaagag tgtcggcaac atcataaatt attgtatttt gctttttata
       61 ttgagctaat acatacaaaa ttattggaaa caatacatag ctgtgaagta tacacaagcc
      121 acaaaaccaa aatataattt caatcaacag agccatgttt tttataaaat tcaatcaatt
      181 ttgaaataat agttgcatta tcaaaatttg acgaaacaaa tgtaactgca tttttttcaa
      241 tggcacaacg taattcatat ttttttatta ataatacaac agcatcaata aattcttgtg
      301 cagaatcagc tatacatatt gattcattat ggactgcatg tataccttcg gcacctattg
      361 aagttgatac aatagcctta cccaaagcca atccttctat aattttaatt ctcatgccac
      421 tacctgcaag taatggaact atcattatag tattttcttg cataaactgc ctggaatctt
      481 ctacctctcc tacaaaagca actcccggca ctttcatttt ttttataaat tgtggtgacg
      541 catttcttcc ggctattgta aatcgtaacc aaggaaattg agcatatata tgtggccaac
      601 atttttcaat aaaccataat ataccttgtt gattgggtgc ccaatccaaa ccgcctatat
      661 gaaatagtga aatagttttt tcatgagatt ccacaggatt aagcgactcg gtaaacacgc
      721 cagttggagc aacaaatacg ggtttagtat tacccatatc tttgtatata tctgcatcaa
      781 attgtgttat tggcaccaat acatcatatg tatcaatcgt tttttgttct aattttttta
      841 ttcgcgacac tagatttgaa atataccatt tttttggcac aaaatgagtt tctgccaata
      901 aacgttccca aattttatat tcaatattat gtgatctata tgatattatt gctttggaat
      961 attttctaat aataggaata tatccacaca tatataatcc ttctatttga ataacatcaa
     1021 aactctgtgt ttgtaatatt gtaataagtt ccttttcaaa ttttttatgt aaaaaacgtg
     1081 tagcagtata tggcattgaa ctaaaaaaca aattatacaa tgctccccaa ggtgttacat
     1141 ttgtttgtaa aggtacagca tgaaattctg ccatagaagc aacagaatca ggaatttcta
     1201 aaatattagt ataatgtttt gacgtattga ttgccgcaac tgttacattg tgttgcaaga
     1261 gataataccc tttaaacatt gtaaatactg caattacacc accatcttta agaggataag
     1321 ggaatttatt acatatttga agaatgttca ctttttgaat gctgtttaaa aaattgtact
     1381 attttcttat atatctgtat aaaaatatct ttggggggag ttattatttt gtcatgtatt
     1441 gccatatatg taaaatatac tcctatcaat aaaataattc ccgtaggata ccacatacct
     1501 tgaaacacag gactttctgc agattttacc gatttttcgc ctatcatact caacacataa
     1561 taaattaagt aaaataagaa tgaaaaaatt accggcacac ctaatccacc ttttcgtacc
     1621 atagctccaa aaggagctcc cacaaaaaac ataattaaac aagcaaaagc caatgtaaat
     1681 tttttatgta actctatctt atgcttattg agtgatatat cttgcatttg ttcattttct
     1741 acagcagtag tcaaaaacgc ttcacccgaa cgagcataat tcaaagcccg aataaaactc
     1801 tgaattttat atgcattatt aaactcatta tatatactgt ctatatttgc atcggtacct
     1861 ttatcaattt tactgcgtga acgatatatg ttattagtaa aataatgatt aaaggaacgt
     1921 tcatttctgt atgccaatgt tgtcttcatt gaatcaatag tagtaagtaa ttgactcata
     1981 ttttgcattt gggaattgtg tttaaacaat tccatatcgg ttctattaaa agcaaacgat
     2041 tctaattcaa acaatacttg ttgaacttta aaatactgtt ctctaaaagg tcgctttgtt
     2101 cgaatataat tatattccaa accttctaca acatcttcat accgcttgcc attatatagt
     2161 gtcaaaatta aatattgttt atctt
//
LOCUS       DAOTOW010000388         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39161_length_2180_cov_2.805647, whole genome shotgun sequence.
ACCESSION   DAOTOW010000388 DAOTOW010000000
VERSION     DAOTOW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39161_length_2180_cov_2.805647"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="P5243_07310"
     CDS             <1..218
                     /locus_tag="P5243_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS19295.1"
                     /translation="KQDYIFRKSRKRIETLFSQLCDQFMIRRNYAKSFEGFKTRILSK
                     ITALTVIQGINKFVFNRNSNNLKINIA"
     gene            422..>2180
                     /locus_tag="P5243_07315"
     CDS             422..>2180
                     /locus_tag="P5243_07315"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HRS19296.1"
                     /translation="MRIKILVVLVSIFFCSSVIAATITSAGSGNWNSTSTWVGGVVPG
                     AGDNVIIANTHNVTANVDITTTGTVTVNSGGTLTLNSGILVNSTINATGAIAWPNWGT
                     IKIGSSANITVSGNISASTTTIEYQNAYVSPSAAKITATGTMAFQTLKSNSSPPKGGS
                     LQATTISIGTYGENEIGVDVYFNGTTTVANYRGYGTTTFTGGTFTVSSSFTANNSGPT
                     NTITSTTHDLIFNVDVVKKGSSLQIDVGNNNVYFNAGFENHWTGDPYSMIVANTVYLT
                     NKIKTEGNAVLYIDANVVQQNNIIVNENASGHIYISGNFNSGAYGTDYQNINGTFFVQ
                     GNSTFNNITGHLNSTAQLVTLGNSTMNGTGTWLNNQDGPLYVGGTLTTNSVTTFQGSA
                     KAYVNTVTGGGSVTPSASYTTAPSSVTYNPFPTITGSSTVCVGATITLIGSGTPHPTT
                     PWTSASTSVATVNSSGVVTGVSAGTSIITYRNSSNMQARLTVTVNATPTITGTTPNSR
                     CGTGTVTLGATASAGTINWYTASSGGTSQGTGTSYTTPSISSTTTYYVDATNAGCTTA
                     SRTAVTATVNAIPSMTSASS"
BASE COUNT          747 a          357 c          406 g          670 t
ORIGIN      
        1 aaaaacaaga ctacattttt agaaaatcaa gaaaacgtat agaaacattg ttttctcaat
       61 tgtgtgacca atttatgatt agaagaaatt atgctaaatc ttttgagggg ttcaaaacaa
      121 gaatattatc taaaatcact gctcttactg ttattcaggg aataaacaaa tttgttttta
      181 atagaaattc aaataatctt aaaataaata ttgcctaaat gcacaacggg ttaatatatt
      241 gtgatacaat ataattataa aacacgcatt ttttgaagga tttattacat gttattttta
      301 gaatataata cgtaacactt tgtttttcag tttgttgttt tttttgtata gatgttgctt
      361 tttgtttttt tgatttgtat ctttacaaag atttatattc agggagataa aaagataata
      421 tatgagaata aaaatacttg tagtacttgt ttcaatattt ttttgtagtt cggtaattgc
      481 ggctactata acatctgcag gttcaggaaa ctggaattca acttcaactt gggttggagg
      541 agttgttccc ggtgccggag ataatgtaat tattgccaat actcataatg ttactgcaaa
      601 tgtagatata accacaacag gaaccgtaac agtaaatagt ggaggaacat taacgctcaa
      661 tagtggtatt ttagttaatt ctacaattaa tgctaccggt gctattgcat ggccaaattg
      721 gggaacaata aaaattggtt cgagtgcaaa tattactgtt tcaggaaata tatcagcttc
      781 aactactact atagaatatc aaaatgcgta tgtaagccca agtgctgcta aaattacagc
      841 taccggtact atggcctttc aaacgttaaa gtctaatagt agtccgccta aaggtggctc
      901 tttacaagca actaccattt ctataggaac gtatggcgaa aatgaaattg gagtagatgt
      961 atattttaat ggaacaacaa ctgttgctaa ctatagagga tatggtacaa caacatttac
     1021 cggtggtacg tttactgttt cgtcaagttt tactgctaat aatagtggcc caactaatac
     1081 aattacctca actactcacg atttaatttt taatgttgat gtggtaaaaa aagggtctag
     1141 tttacaaata gatgttggca ataataatgt atattttaat gcaggatttg aaaatcactg
     1201 gactggagat ccatatagta tgatagtggc aaatactgta tatcttacta ataagattaa
     1261 aactgaaggt aatgcagtgt tgtatattga tgccaatgtt gtacaacaaa ataatattat
     1321 cgttaacgaa aatgcttcgg ggcatattta cataagcgga aattttaata gtggagcgta
     1381 tggtactgat tatcaaaaca taaacggtac cttttttgtg caaggtaata gtacttttaa
     1441 taatataacc ggtcatttaa atagtactgc acaattagtt actcttggaa attcaacgat
     1501 gaacggaaca ggaacatggc ttaacaatca agatggtccg ttgtatgttg gggggacgtt
     1561 aacaaccaat tcagtaacaa cttttcaggg atcagcaaaa gcgtatgtaa atacggtaac
     1621 aggaggaggt tctgtaactc catcagcttc gtacactact gctccgtcat cggttacata
     1681 taatcctttc ccaactatta ccggttcttc aacagtatgt gtgggtgcaa ctataacact
     1741 tatcggttcc ggtactccac accctactac accgtggaca tcggcatcaa caagtgttgc
     1801 aacggttaac agcagtggag ttgttacagg tgtgtctgca ggaactagca tcataaccta
     1861 tagaaatagt tcaaatatgc aagctcgact tactgtaaca gtaaatgcta ctccaacaat
     1921 aacaggtacc actccaaatt cgcgttgtgg cacaggaacg gtaacattag gagctacagc
     1981 atcggcaggt actattaatt ggtatacggc atcgagtggg ggaacatcac aaggaacagg
     2041 aacatcatat actacaccga gtatatcgtc tacaaccaca tattatgtag atgcaacaaa
     2101 tgcaggctgt acaacagcaa gtagaacagc tgtaacagct accgtaaatg ccataccaag
     2161 tatgacaagt gcatcatcaa
//
LOCUS       DAOTOW010000389         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39203_length_2178_cov_3.159209, whole genome shotgun sequence.
ACCESSION   DAOTOW010000389 DAOTOW010000000
VERSION     DAOTOW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39203_length_2178_cov_3.159209"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="P5243_07320"
     CDS             <1..861
                     /locus_tag="P5243_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19297.1"
                     /translation="PVTFHYYYIKPKFISLDAGFNTFLRDASANYNGEGGSGNPVGAT
                     ISDIDYTNGNRKGADVRTELNIEGLKINAAYGISQEVEHLTHIISYNHRINGIMFARV
                     YAYQTNLGENESFNTTYRGYYETSSIDTTNPTYISWKKLNFNTLECNLKYKVNILNKP
                     LYLFYLGSLSTMQQDLSLYPVVSDKAYMQIQYHEFESYYKVLSKVALAGYFGLETMKG
                     NQYMKHGPNLKGNPTNVLGTGLGLGFDAELSNSANLYVRGRWFDYTDRNNTFYTYKGF
                     EGTVELRVYF"
     gene            898..>2178
                     /locus_tag="P5243_07325"
     CDS             898..>2178
                     /locus_tag="P5243_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19298.1"
                     /translation="MKKIPIIIALSTIGLCTYAQNQKVTFNGAARSSIESNWLPQTDT
                     TNIDQATGGNTVVDLRLDIKPSDNIRLKTDLRFANPMGGFWGQGATIQLRHLSLKGVA
                     GNIFKYRFGDIDVKMTPYTLYNYDGELSKNEAEIFRFTRDINQEENFNYGNFWHQQGV
                     DGAFRLGFDKFIQSADISGFMVRNRSVEGNIIPDRLQGGTVVKCNINSGSYIGFNYIS
                     LFDLPKTVSNDTKEKYQNSVSTAIARFTVKDFILFGEAGASVSAYTDTSAVALKDIAG
                     NFFEAGITKSLLSKQLTLQASYRRVSDDFISAGAQTKRINYSATPQVLPLVSEKSFTR
                     SVGLFDLVKDQTIYNPSITPQLMPYDYSLNHVTPYGKATPNRKGISVAIDYTDSAHVY
                     ASGLSVDLLKDVRGEGTEELRNYLLFNWNGMLAIH"
BASE COUNT          763 a          312 c          392 g          711 t
ORIGIN      
        1 cctgttacat ttcattatta ttatataaaa cccaagttta taagtttaga tgcaggtttc
       61 aatacgtttt tgcgtgatgc atcggcaaat tataatggtg aaggaggaag cggaaatcct
      121 gtaggtgcta caatttctga tattgattat acaaatggta atcgtaaagg ggctgatgtg
      181 cgaacagaac taaatataga aggattaaaa ataaatgcgg catatggtat atcgcaagaa
      241 gttgaacatt taacgcatat tatttcttat aaccaccgaa tcaatggaat tatgtttgcc
      301 cgtgtgtatg cgtatcaaac taatttgggt gaaaatgaaa gttttaatac aacatataga
      361 ggatattatg aaactagttc aattgatacc actaacccaa catatatatc atggaaaaaa
      421 ttaaatttca atacactcga atgcaattta aaatataaag tcaatatatt gaataaacca
      481 ttgtatttgt tttatttagg ttcactttct accatgcaac aagatttatc gttgtatccg
      541 gtggtttcag acaaagcata tatgcaaatt caatatcatg aatttgaatc atattacaaa
      601 gtgttatcaa aagttgcatt ggcaggatac tttggtttag aaactatgaa aggaaatcaa
      661 tatatgaaac atggaccaaa ccttaaagga aatcctacaa atgtattagg aaccggttta
      721 ggtttgggat tcgatgcaga attatcaaat tctgccaatt tgtatgtgcg tggacgttgg
      781 ttcgattata ctgatagaaa caatactttc tatacttaca aaggatttga aggtactgtt
      841 gagttacgag tttattttta aatacagtaa aaataatttt attaagaata tatgcgaatg
      901 aaaaaaattc cgattattat tgctctaagt actataggat tgtgtacata tgcacaaaat
      961 caaaaagtta cttttaacgg agctgcacgt agttctattg agagtaattg gttacctcaa
     1021 accgatacta caaatattga ccaagctaca ggaggaaaca cagtagttga tttacggtta
     1081 gatataaaac catcagataa tattcgttta aaaacagatt tgcgatttgc taatcccatg
     1141 ggtggttttt gggggcaagg agcaactatt caactccgtc atttatcatt aaaaggtgtt
     1201 gcaggtaata tttttaaata cagatttggt gatattgatg taaaaatgac cccatatacg
     1261 ttgtataatt atgatggaga attgtctaaa aatgaggctg aaatatttag atttactcgt
     1321 gacattaatc aagaagaaaa ctttaattat ggaaattttt ggcatcaaca aggagttgat
     1381 ggtgcttttc gtttaggatt tgacaaattt attcaatctg ccgatatttc cggttttatg
     1441 gtacgcaata gaagtgttga aggaaatata attcctgata gattgcaggg aggaactgtt
     1501 gtgaaatgta atataaattc cggctcatat ataggattca actacatttc attgtttgat
     1561 ttacccaaaa cagttagtaa cgatactaaa gaaaaatacc aaaattcggt atcaaccgct
     1621 atagctcgtt ttactgttaa agattttatt ttgtttggtg aagcaggtgc ctcagtatcg
     1681 gcttataccg atacatctgc tgttgcatta aaagatattg caggtaattt ttttgaggct
     1741 ggaattacaa aatcattgct ttcaaaacaa cttacattac aagcatcata tcgtagagta
     1801 agtgatgatt ttataagcgc cggagcacaa acaaaacgaa ttaattatag cgcaacacca
     1861 caagtattac ctttagtatc agaaaaatca tttactcgtt ctgttggatt gtttgattta
     1921 gtaaaagatc aaactatata caatccgtct attacaccac agcttatgcc atatgattat
     1981 tcattaaatc atgttacccc atatggtaaa gcaactccta acagaaaagg gatttctgtt
     2041 gcaatagact ataccgattc agcacacgta tatgcttccg gtttaagtgt tgatttactt
     2101 aaagatgtaa gaggcgaagg taccgaagaa ttacgtaatt atttattgtt taactggaat
     2161 ggaatgcttg caattcat
//
LOCUS       DAOTOW010000390         2177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39228_length_2177_cov_3.214892, whole genome shotgun sequence.
ACCESSION   DAOTOW010000390 DAOTOW010000000
VERSION     DAOTOW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39228_length_2177_cov_3.214892"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2177
                     /gene="rpoB"
                     /locus_tag="P5243_07330"
     CDS             <1..>2177
                     /gene="rpoB"
                     /locus_tag="P5243_07330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRS19299.1"
                     /translation="ILEIFDLAEEVKVSKSGLKKVVGRKLAARVLKSWIEDFVDEDTG
                     EVVSIERNEVIIEREVIIDNDHIDEIVDSGQKTILIHREDQNSADYDIIYNTLQKDPC
                     NSEKEAVFHIYRQLRNAEPPDEATARDVIDKLFFSDKRYDLGEVGRYRINTKLYAGAD
                     VEAEKEDIRVLTKRDIIHIIKYLIQLINSKADVDDIDHLSNRRVRTVGEQLHNQFGVG
                     LARMARTIRERMNVRDNEVFTPIDLINSKTLSSVINSFFGTNQLSQFMDQTNPLGEMT
                     HKRRLSALGPGGLSRERAGFEVRDIHYTHYGRLCPIETPEGPNIGLISSLCVYAKINE
                     LGFIETPYRKVTNGVVDLSDEGVVYLSAEEEEGKIIAQANAPLKDSGEFVNNRIKSRL
                     EADYPLSTPEEIDLMDVAPNQITSIAASLIPFLEHDDANRALMGSNMMRQSVPLLKAE
                     APIVGTGIERQVIKDSRIQVTAEGDGVIEYVDANEIRVRYDQTEEEAFISFEDNLKTY
                     SIIKFRKTNQNTCINLRPIVKKGERVVKDQCLTEGYSTQNGELAIGKNLKVAFMPWKG
                     YNFEDAIVISERIVREDILTSIHIDEHLLEVRDTKRGLEEFTSDIPNVSEEAIKNLDE
                     NGLIRVGANIKPGDILIGKITPKGESDPSPEEKLLRAIFGDKAGDVKDASLKARPSLY
                     GTVIDKKLFTRTIKDRKSRANEKPILTKFKADFDVEADKLKAV"
BASE COUNT          773 a          323 c          413 g          668 t
ORIGIN      
        1 cattcttgaa atttttgatt tggccgaaga ggttaaagtt tcaaaaagcg gattaaaaaa
       61 agtagttgga agaaaattag cagctcgtgt tttaaaatca tggattgaag acttcgtaga
      121 tgaagatacc ggtgaggtag tatctattga gcgaaatgaa gtgataattg aacgtgaagt
      181 aataattgat aatgatcata ttgacgaaat agttgattct ggtcaaaaaa caattcttat
      241 tcatcgcgaa gaccaaaatt cggctgatta tgatataatt tataatactc ttcaaaaaga
      301 tccttgtaat tcagaaaaag aagcagtatt tcatatttat cgtcaattgc gtaatgcaga
      361 gccaccagat gaggctactg ctcgtgatgt gatagataaa ttattctttt cggataaacg
      421 ctatgattta ggtgaagttg gtcgctacag aatcaatact aaattatatg caggagccga
      481 tgttgaagca gaaaaagaag atattcgtgt tttaacaaaa cgagatataa ttcatatcat
      541 taaatattta attcagctta ttaattcaaa agctgatgtt gatgatattg accatttaag
      601 taaccgtcgt gtacgaacag ttggtgaaca attacataat caatttggag taggattggc
      661 tcgtatggca cgtactattc gtgaacgaat gaatgttcgt gataatgaag tgtttactcc
      721 aattgatttg ataaattcaa aaacattatc ttctgttatc aattcattct ttggtaccaa
      781 ccaactttct cagtttatgg atcaaaccaa tccattggga gaaatgactc ataaacgtcg
      841 tttatcggcg ttaggtcccg gtggtttatc tcgcgaacgt gcaggttttg aggtacgtga
      901 cattcattat acacactatg gtcgtttatg tccgattgaa actcctgaag gtccgaacat
      961 tggtcttatt tcttcattgt gtgtatatgc aaaaataaac gaacttggtt ttattgaaac
     1021 tccatatcgt aaagttacca atggtgtggt agatttatct gacgaaggtg tagtttattt
     1081 aagtgccgaa gaagaagaag gtaaaataat tgctcaagct aatgcacctt taaaagattc
     1141 aggtgaattt gttaataaca gaatcaaatc acgtttagaa gcagattatc cactttcaac
     1201 tcctgaagaa attgatttaa tggatgttgc tccaaaccaa attacctcta ttgcagcttc
     1261 acttattcct ttcttagaac atgacgatgc aaaccgtgca ttgatgggat caaacatgat
     1321 gcgtcaatct gttccattat taaaagcaga agctccaatt gtaggaacag gtatagaacg
     1381 tcaagttatt aaagattctc gtatacaagt aactgccgaa ggtgatggag ttattgaata
     1441 tgttgatgct aacgaaattc gagtacgtta tgaccaaacc gaagaagaag catttataag
     1501 ttttgaggat aatttaaaaa cttacagtat aattaaattc cgaaaaacta atcaaaatac
     1561 atgtatcaat cttcgcccaa ttgttaaaaa aggtgaacga gttgttaaag atcaatgttt
     1621 aacagaaggg tattctacac aaaacggaga attagcaatt ggtaaaaact taaaagttgc
     1681 tttcatgcct tggaaagggt acaactttga agatgcgatt gttatttctg aacgtatagt
     1741 tagagaagac attctaactt ctatacatat agatgaacat ttattagaag tacgtgatac
     1801 caaacgaggt ttagaagaat ttacatctga tattccaaac gtaagcgaag aagcaattaa
     1861 aaatcttgat gaaaacggat taatacgagt tggagctaac ataaaacctg gtgatatact
     1921 tattggaaaa attactccta aaggagaatc agacccttca cctgaagaaa aattattacg
     1981 cgctatattt ggtgacaaag caggtgatgt aaaagatgct tcactcaaag ctcgtccttc
     2041 gttatatggt actgttatag ataaaaaatt atttactcgt actataaaag atcgtaaatc
     2101 gagagccaac gagaaaccta tcttaacaaa attcaaagca gattttgatg tagaagcaga
     2161 taaattaaaa gctgttt
//
LOCUS       DAOTOW010000391         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39424_length_2170_cov_3.230260, whole genome shotgun sequence.
ACCESSION   DAOTOW010000391 DAOTOW010000000
VERSION     DAOTOW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39424_length_2170_cov_3.230260"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2170)
                     /locus_tag="P5243_07335"
     CDS             complement(<1..>2170)
                     /locus_tag="P5243_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS19300.1"
                     /translation="IIRNKKITTLCQTPHHSIVCTGIGLTYIYTCTYNKQKASSTYDS
                     VYSNVSVSKLFLDSKDRLWIVHSINKISYITNGITTHIGLASFIPQMLCVDIIEYNGT
                     ILLGTSQGIFEFAQKRKSFIPLQNNPGKITDVYVTNFVISKHNELLVASDNRGVLLYT
                     QTYGLQQYISKQQHNQIAHNSVYTVFQDASNTLWISTLGAQLQQIMLSVQPFGKHIYT
                     QNSNLPDNMVKTLLVDSYKNLWIGTDGGGICYIADFPKQTSVTIIPNSPKKIMTFEQQ
                     GTKLWVGTYINGLYELQIENKRLIHHPISINGTIVQNIFDIELIDSERMLLATLGTGL
                     IEYNSKTNKAIRIQNVQYNSKTASIDAFLSSLCVDAQNNIWIGGSGQGIVVCNRQMQA
                     LHFYNSFDVPLSNDIVNAIAHDSKGTLWVATAQGINAINTKDFKVTQFTSALYPMFQN
                     SYAVTYYNNKVWVGTAQNLVTMDISTNQIQTYSVINGIFSIKNQCNFICFSDSLAIIA
                     GNNGVSWIPISKLETLTYPTIQKNAVYIRVQGELIESKPRIVLPYYKNTLQISFPVIE
                     FQHNHDIRYSYHISGSMEEIGLLENSYEMVLANMSPGTYTITLQAYYVQHINIIHSYT
                     FQIIIRPPFWKTWWFIVLLIITLICIVIYSVRLRWQRMKRQAFILEQKVMERTKEIQS
                     QKEELQVQAEKLQEINDELVRTNSEQSKLFEILSKTAVSKQ"
BASE COUNT          684 a          338 c          320 g          828 t
ORIGIN      
        1 ttgcttgctt actgctgttt tactcaaaat ttcaaataat ttggattgtt cggagttggt
       61 gcgtactaat tcgtcattta tttcttgtaa tttttctgct tgtacttgta gttcttcttt
      121 ttgactttga atctcttttg ttcgctccat cactttttgt tcaagtataa atgcttgcct
      181 tttcattctt tgccatcgta aacgaacact atatattaca atacaaatga gtgtaataat
      241 tagtaaaact ataaaccacc atgttttcca aaacggaggg cgtattataa tttgaaatgt
      301 atatgaatga atgatattta tatgttgtac ataatatgcc tgtaaagtaa tagtatatgt
      361 gccgggcgac atatttgcca ataccatttc atatgaattc tcaagcaacc ctatttcctc
      421 cattgagcca ctaatatgat atgaatacct aatatcatga ttatgttgaa attctattac
      481 aggaaatgat atttgtagtg tatttttata atatggtaaa actatgcgtg gtttagattc
      541 tattaattct ccctgtaccc gaatataaac agcatttttc tgtatagtag gatatgtcaa
      601 tgtttcaagt tttgaaattg gaatccaact cactccgtta tttccggcaa ttattgccaa
      661 tgagtcagaa aaacaaataa aattacactg attttttatt gaaaaaattc catttataac
      721 tgagtatgtt tgaatttgat tggttgaaat atccatagtt accaaatttt gagcagtacc
      781 aacccaaact ttgttattat aatacgttac cgcatacgaa ttttgaaaca ttggatataa
      841 agcacttgta aattgtgtga ctttgaaatc ctttgtatta attgcattta taccttgtgc
      901 agttgctacc cataaagtac cttttgaatc gtgtgcaata gcatttacta tatcattaga
      961 caagggcaca tcaaatgagt tataaaaatg caatgcctgc atttgtctat tacacactac
     1021 aataccttga ccgctaccac ctatccatat attattttgg gcatctacac acaacgacga
     1081 caaaaacgca tctattgacg ctgttttcga attatattgt acattttgta tgcgtattgc
     1141 tttattagtt ttactattat attctatcaa acctgtaccc aatgttgcaa gcagcattcg
     1201 ttcagaatct attaattcta tatcaaatat attttgtact atggtaccgt ttatagaaat
     1261 aggatgatgt attaatcgtt tattttctat ttgcagttca tacaaaccat taatatatgt
     1321 gccaacccat agtttagttc cttgttgttc aaatgtcatg attttttttg gtgaattagg
     1381 aataattgta acacttgttt gtttaggaaa atcagcaata taacatattc ccccaccatc
     1441 ggttcctatc catagatttt tatatgaatc tactaaaagg gtttttacca tattatcggg
     1501 caaattgctg ttttgtgtat atatatgttt accaaagggc tgaacactta acattatttg
     1561 ctgcaattga gcacccagag tacttatcca aagagtatta gatgcatctt gaaaaacagt
     1621 atatacagaa ttatgagcta tttgattatg ttgttgtttt gaaatatatt gttgcaatcc
     1681 atatgtttga gtgtatagca acacgcctct attatcagaa gctaccaaaa gttcattatg
     1741 ttttgaaatt acaaaattgg ttacatatac atctgttatt ttgcctggat tattttgcaa
     1801 gggtataaaa gattttcttt tttgagcaaa ttcaaaaatt ccttgtgagg tgcctaataa
     1861 tattgtacca ttatactcta taatatctac acataacatt tgagggataa aagatgctaa
     1921 accaatatga gtggtaattc cattggtaat atatgatatt ttatttattg aatgtacaat
     1981 ccataatcgg tcttttgaat ccaaaaacaa ttttgaaact gatacattag aatatactga
     2041 atcatacgta cttgaagctt tttgtttatt ataagtacat gtatatatat atgtcaatcc
     2101 aatacctgta catactattg aatggtgtgg tgtttggcat agcgtagtta tttttttatt
     2161 tcttataatt
//
LOCUS       DAOTOW010000392         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39704_length_2158_cov_3.138849, whole genome shotgun sequence.
ACCESSION   DAOTOW010000392 DAOTOW010000000
VERSION     DAOTOW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39704_length_2158_cov_3.138849"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..421)
                     /locus_tag="P5243_07340"
     CDS             complement(74..421)
                     /locus_tag="P5243_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase domain-containing protein"
                     /protein_id="HRS19301.1"
                     /translation="MQSHSLSDKWQFITMQRVGSNYEMYVNGTLVSLTKGTLSSDPTD
                     EVQNMMHNLFIGTFAGNNPEYYFKGYNFSGKIDDINFYTRALLPCEIKKLYSSKTSRI
                     KKKYNFRFCIHKK"
     gene            complement(382..906)
                     /locus_tag="P5243_07345"
     CDS             complement(382..906)
                     /locus_tag="P5243_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19302.1"
                     /translation="MKCFLIVCYSCISILLYAQKNNHIFYLPFNGNTLDETGNGNNAV
                     NHGATLTTDRFGNQNSAYEFDGIDDFMQITPVSDLSSVGDYSISLWILHKNWKGTTQT
                     NNIERQYIFDAHASSATATGNDIFKPGFVTAIDYNYTSNTENLIDLMYIKLILFRITK
                     QKCNHIRYQINGNL"
     gene            1136..>1443
                     /locus_tag="P5243_07350"
     CDS             1136..>1443
                     /locus_tag="P5243_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19303.1"
                     /translation="MKRLVVVFCVVGLSFILHAQNLTISGGSEHSVMICGKGLVYAWG
                     NNKIANPPMNNLLGLKDAAQAAAAFVTKPTEVQLPTGVTFKQVDAGSGAFNIALACNN
                     V"
     assembly_gap    1444..1453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1454..>2158
                     /locus_tag="P5243_07355"
     CDS             <1454..>2158
                     /locus_tag="P5243_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19304.1"
                     /translation="MWGANDFKQLGQGNSSTAVSLSVPTPVVKGATPGSTAPGMSAYL
                     GNATFVSASTSTAYAILDDKRVVAWGSNGYKQLGNPAVTAATWGEPTYMKMKATATSP
                     IVDITNVIFLDAGDEQVLALVDDGVIGDGVGTVYSCGGSAYNAADLVLGRPATNTDVF
                     MPVLKQDGTPLSNIKMVSAGDSHGIAVDATTGYVWSWGNDGWGCLTGTTFPNPSTQGN
                     RASRVVSGEYKTISGLD"
BASE COUNT          714 a          350 c          366 g          718 t
ORIGIN      
        1 taagttacaa atttacactt atttttcctg tcctaatttt ggggtgtatt atatactctt
       61 attatacaaa cattcatttt ttatgaatac aaaacctaaa attgtatttt tttttaattc
      121 tacttgtttt tgaagaatat aattttttaa tctcgcaagg caaaagcgca cgagtataaa
      181 aattaatatc atcaattttt ccactaaaat tatacccttt aaaataatat tcaggattat
      241 tgcctgcaaa tgtccctata aatagattat gcatcatatt ttgcacctca tctgtaggat
      301 ctgatgataa tgtaccttta gttaatgata ccaaagtacc atttacatac atttcataat
      361 ttgaacctac tcgttgcata gttataaatt gccatttatc tgataacgaa tgtgattgca
      421 tttttgcttc gtaatacgga ataggatgag tttgatatac atcaaatcta ttaaattctc
      481 tgtatttgat gtataattat aatcaattgc tgttacaaat ccaggcttga atatatcatt
      541 tccggttgct gttgctgaag aagcatgtgc atcaaaaata tattgacgct ctatattatt
      601 agtttgagta gttcctttcc aatttttatg taaaatccac aatgaaattg aataatcacc
      661 aacactcgac aaatcagata caggagtaat ttgcataaag tcatcaatac catcaaattc
      721 ataggcagaa ttttgattgc caaatctatc agtagttaat gttgctccat gatttactgc
      781 attattccca ttcccggttt catctaaagt attcccattg aaaggtaaat aaaatatatg
      841 attatttttt tgtgcataaa gtagtatgct tatacaacta taacatacaa ttaaaaaaca
      901 tttcattcat ataattatta catattaaac aaatatacgt attattaact aatttcatac
      961 caaaatttaa tatttaacat tttttatttt tctgatattc agcaaaaaaa aaattctgtt
     1021 taaaaaattt gtcatttcat ataaaaaaat catgcaacct ttttaaattt tttaagtctt
     1081 gtgtgtgcat tcacaaaggt gtgtgcagta ttatattgaa atatttttaa tttttatgaa
     1141 acgattagta gtagtatttt gtgtagttgg tttaagtttc atactgcacg ctcaaaattt
     1201 aacaatttcg ggtggatctg aacactcggt aatgatctgt ggtaagggtt tagtatatgc
     1261 atggggtaat aataaaattg ccaatccacc aatgaacaat ttattaggtt taaaagatgc
     1321 agcacaagcg gcggcggcat ttgtaactaa acctaccgaa gttcaacttc ctacaggagt
     1381 aacatttaaa caagtagatg ccggctctgg agcatttaac attgcacttg catgtaataa
     1441 tgtnnnnnnn nnnatgtggg gtgctaatga ttttaaacaa ttaggacaag gaaattcttc
     1501 aacagctgta agtttatcgg tgccaacacc ggtagtaaaa ggtgcaacac cgggtagtac
     1561 agctcccgga atgagtgctt atttaggaaa tgcaacattt gtttctgctt ctacatcaac
     1621 agcatatgct atattagatg acaaacgagt tgtagcatgg ggttctaatg gttataaaca
     1681 attaggaaat ccggcagtaa cagcagcaac atggggagaa cctacctata tgaaaatgaa
     1741 agctaccgcc acatctccta ttgtagacat aacaaacgtt atttttcttg atgcaggaga
     1801 tgaacaagtg cttgcacttg tagacgatgg cgtaataggc gatggtgttg gaaccgtata
     1861 ttcttgcgga ggtagtgcat ataatgctgc agatttagta ttaggtcgcc ctgcaaccaa
     1921 caccgatgta tttatgccgg tacttaaaca agacggtaca cctctttcga atataaaaat
     1981 ggtttcggcc ggagactcgc atggtatagc agtagatgcc actaccggat atgtatggag
     2041 ttggggtaac gacggttggg gatgcttaac aggaactaca ttcccaaatc caagtacgca
     2101 aggaaaccga gcaagtagag ttgtttcggg tgaatacaaa acaatttccg gcctcgat
//
LOCUS       DAOTOW010000393         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39730_length_2157_cov_3.106089, whole genome shotgun sequence.
ACCESSION   DAOTOW010000393 DAOTOW010000000
VERSION     DAOTOW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39730_length_2157_cov_3.106089"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="P5243_07360"
     CDS             <1..1257
                     /locus_tag="P5243_07360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS19305.1"
                     /translation="YFCIQLNETMKFQDYTFCSEIKQNLEELGYKKPTDIQYKAIPSI
                     LQGEDVLAIAQTGTGKTAAFVIPIIEILHNRTRKSRQPHIQCVIMEPTRELVIQTARV
                     CNTLAKNTTIKTLGLFGGVEQDPQIAVLTKGVSILVATPGRLFDLISQGYVSLQQLKI
                     LVVDEADHMLDLGFIEDIKQLLRHIPRYRQTLFFSATITPQIKDLAYSLVHKPVRIQI
                     SPKDPVSKNVDHYVAFIEMDDKRFFLEQFIKEHQENKILVFVRTKVRAERVKAAMARV
                     QIHTETIHGDKPQKERNHIMKEFAAGTIQILIATDISARGIDIPNIDYVINYDMPEDA
                     ENYVHRIGRTGRGTKRGSSIWFCSESEKELLTHIEEFISKKITVIDIDKQTYKQTILQ
                     SIDTSTPLQDILKEIEYIDSKRKKKK"
     gene            1301..2098
                     /gene="kdsA"
                     /locus_tag="P5243_07365"
     CDS             1301..2098
                     /gene="kdsA"
                     /locus_tag="P5243_07365"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRS19306.1"
                     /translation="MPLIQSYESHNFFLMAGPCIIEDEGMAMQIAEKIKQICEKLEIP
                     YIFKGSYRKANRSRIDSFTGIGDEKALSILAKIRNELHIPVVTDIHSAEEAPIAAAYV
                     DVLQIPAFLCRQTDILVAAAKTGKCVNIKKGQFVSAESMKWAAQKVVDSGNSHILLTD
                     RGTMFGYHDLIVDYRNIPEMQSHGFPVILDITHSLQQPNQSSGVSGGKPQLIETIAKA
                     GVAVGVDGLFIETHPFPAQAKSDGSNMLQLDLLEGLLRKLVAIRQVL"
BASE COUNT          821 a          342 c          374 g          620 t
ORIGIN      
        1 tacttttgca tacaattaaa cgaaactatg aaatttcaag attatacatt ttgcagcgaa
       61 atcaaacaaa acttagaaga attaggatat aaaaaaccaa cagatattca atacaaagct
      121 attccttcaa tattacaagg agaagatgtt ttggctatag cacaaacagg aaccggaaaa
      181 acagcagctt ttgtaattcc aataattgaa attttacata acagaactcg aaaatctcgt
      241 caaccccata ttcaatgcgt aataatggaa ccaactcgtg aacttgtaat tcagactgca
      301 cgagtatgca atacattggc aaaaaacaca actataaaaa cattagggtt gtttggtgga
      361 gtagaacaag accctcagat tgcagtgctt acaaagggtg ttagcatatt ggtagcaaca
      421 ccggggcgat tatttgattt aataagtcaa ggatacgtgt cattacaaca attaaaaatt
      481 ttggtagttg acgaagctga ccatatgctt gatttaggtt ttattgaaga tattaaacaa
      541 ctgttgcgac atataccacg atataggcaa actttatttt tttcggctac tatcactcct
      601 caaattaaag atttagcata ttctcttgta cataaaccgg tacgtattca aatatcccca
      661 aaagaccctg tttcaaaaaa tgtagatcat tatgtagcat ttattgaaat ggatgataaa
      721 cgtttttttc ttgaacaatt tataaaagaa catcaagaga ataaaatatt ggtatttgtt
      781 agaacaaaag tacgggcgga acgagtaaaa gcagcaatgg caagagttca aatacacaca
      841 gaaacaattc atggtgacaa accccaaaaa gaacggaatc acataatgaa agaatttgct
      901 gcaggcacta tacaaattct tatagcaacc gatatttcgg cacgtggtat tgatattcca
      961 aatattgatt atgttataaa ttacgacatg cctgaagatg ctgaaaatta cgtacatcgt
     1021 ataggcagaa caggaagagg tactaagcgt gggtcttcaa tttggttttg tagcgaatca
     1081 gaaaaagaat tattaaccca cattgaagaa tttatatcaa aaaaaattac tgtaattgac
     1141 atagataaac aaacatacaa acaaacaatt ttacaatcta ttgacacaag tacaccattg
     1201 caagatattt taaaagaaat tgaatacata gattcaaaac gcaaaaagaa aaaataactt
     1261 atagtatata ataatgaaaa caaatgctct tatatcaagc atacctttga ttcaaagcta
     1321 tgaatcacat aatttttttc ttatggcagg tccttgcatt atagaagacg aaggtatggc
     1381 aatgcaaata gccgaaaaaa ttaaacaaat ctgcgaaaaa ctggaaatac cttatatttt
     1441 caaaggttcg tatcgcaaag caaacagatc aagaattgat tcatttaccg gtataggcga
     1501 tgaaaaagct ttatctatat tagcaaaaat cagaaatgag ttgcacatac cggtagttac
     1561 tgatattcat tctgcagagg aagccccaat agctgctgca tatgttgatg ttttacaaat
     1621 acctgcattt ttgtgccgac aaaccgatat attggttgct gcagcaaaaa caggaaaatg
     1681 tgtaaatata aaaaaggggc aatttgtatc ggcagaatct atgaaatggg ctgctcaaaa
     1741 agtcgtagat tcaggtaatt cacacatact cttaacagat agaggaacaa tgtttggcta
     1801 tcatgattta attgtagatt acagaaatat acccgaaatg caatcacatg gattcccggt
     1861 aatattagat attacccatt ctcttcagca accaaatcaa agttcgggag tttccggagg
     1921 gaaaccgcaa ttaatagaaa caattgcaaa agcaggtgtt gctgtaggag ttgacggttt
     1981 atttattgaa acacatccat ttccggctca agcaaaatct gatggaagca atatgttgca
     2041 attagattta ttagaaggat tattacgaaa attagtagct attcgtcaag ttttatagtg
     2101 tatgtataaa ccattatata gtattgtttt ttgtattgca ggggtatttt taggcac
//
LOCUS       DAOTOW010000394         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39734_length_2157_cov_2.672693, whole genome shotgun sequence.
ACCESSION   DAOTOW010000394 DAOTOW010000000
VERSION     DAOTOW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39734_length_2157_cov_2.672693"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="P5243_07370"
     CDS             <1..494
                     /locus_tag="P5243_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRS19307.1"
                     /translation="VDALLAAGADKITINSAAVYTPQLVTDLAKQFGSQCIVVAIDTK
                     LYNNTWMVHTHGGKKPTDIKTIDWVQEIYNRGAGEILLTSMNHDGTKNGFAHEITRTV
                     SEMVDIPVIASGGAGSMEHFKEVFEQGKADAALAASIFHFKEIGIPELKQYLHNEGIS
                     VRL"
     gene            552..1148
                     /gene="hisIE"
                     /locus_tag="P5243_07375"
     CDS             552..1148
                     /gene="hisIE"
                     /locus_tag="P5243_07375"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRS19308.1"
                     /translation="MNIDFTKTTDGLIPAIIQDSHTQVVLMLGYMNKESFEKTVATKK
                     VTFYSRSRKTLWTKGETSGNFLHVVSILVDCDNDTILIKARPDGPTCHTGNDTCFNET
                     NTPAIDFLQYLENVIQTRKKQPVEGSYTNKLFEKGVNKIAQKVGEEAVELIIEAKDTN
                     EELFLGEAADLMYHYLVLLAQKGYSLKDVTQVLEKRHK"
     gene            1239..1922
                     /locus_tag="P5243_07380"
     CDS             1239..1922
                     /locus_tag="P5243_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRS19309.1"
                     /translation="MPKVSIQEIVTTGKLFELLTDDEKQLILNNSTHLRFKKGETICK
                     QGAFSNHIMVITEGLAKTYLEGSNDKNLIIDILKPLNWIGLSSIYKEPTYNFTAATIV
                     DSSVCFIEKKIFVEILANNSAFANQIIGKLCRSSKHFFDKITSLGNKQLHGRFADALL
                     YLSKKVAKSPTIDLSITRKEIGELAGISTESAIRLLSELNHDGIIKLEGKVIIILKEN
                     LLEKLSQIG"
     gene            1924..>2157
                     /locus_tag="P5243_07385"
     CDS             1924..>2157
                     /locus_tag="P5243_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HRS19310.1"
                     /translation="MDSILSLEPQIVWKHFSDICSIPHPSKHEDVLLAHIISFAKQHN
                     LPYKQDSIGNICITKPATKGHEHTETVCLQAHVD"
BASE COUNT          850 a          335 c          341 g          631 t
ORIGIN      
        1 atgtagatgc tctacttgct gcaggtgccg ataaaattac aataaattcg gcagctgtgt
       61 acaccccaca acttgtaacc gatttagcaa aacaatttgg ttcacaatgt atagtagtag
      121 ctatagacac aaaattgtat aataatactt ggatggtaca tactcatggt ggtaaaaaac
      181 ctaccgatat taaaacaata gattgggtac aagaaatata caaccgaggt gctggggaaa
      241 ttttacttac atcaatgaat cacgatggaa ctaaaaacgg atttgcacat gaaattaccc
      301 gtaccgtttc tgaaatggta gatatacctg taatagcttc gggtggagca ggctccatgg
      361 aacacttcaa agaagttttt gaacaaggta aagcagatgc tgcattagct gccagtattt
      421 ttcacttcaa agaaattgga atacccgaat taaaacagta tttgcacaat gagggtataa
      481 gtgtaagatt atagattagt gattttactc tcataattta aactaagaaa ttaaaattaa
      541 gaattaacaa catgaatata gactttacaa aaacaacaga cggattaatt ccggcaatca
      601 ttcaagattc acacactcaa gtagttctca tgcttggtta tatgaataaa gaatcgtttg
      661 aaaaaactgt agcaacaaaa aaagttactt tttatagtcg ttcacgaaaa acattgtgga
      721 caaaaggaga aacttcagga aactttttac atgtagtatc aatattagta gattgcgaca
      781 acgacacaat acttataaaa gcacgtcctg acggaccaac ttgtcacaca ggtaacgata
      841 catgctttaa tgaaacaaat acacctgcaa tagatttttt gcaatattta gaaaatgtaa
      901 tacaaaccag aaaaaaacaa ccggtagaag gttcatatac caataaacta tttgaaaaag
      961 gtgtcaataa aattgcacaa aaagtaggtg aagaagcagt agaattaata atagaagcca
     1021 aagatactaa tgaagaatta tttttgggcg aagcagccga tttaatgtac cattatttag
     1081 tattactggc acaaaaagga tattcactga aagatgtaac acaagttctt gaaaaacgtc
     1141 ataaataaac ctgagtgtac aacacacata ttgagttttt caaataaatg tttatattta
     1201 tagaaattca atgttattaa cctaatatat aagtatctat gccaaaagtt tcaatacaag
     1261 aaatagtaac caccggaaaa ctatttgaat tgttaaccga tgatgaaaaa caacttattc
     1321 ttaataacag tactcactta cgattcaaaa aaggagaaac tatttgcaaa caaggtgctt
     1381 tttcaaatca tataatggta ataacagaag gattggcaaa aacatattta gaaggttcaa
     1441 acgataaaaa tttaatcata gacatattaa aacctcttaa ttggattggt ctttcttcaa
     1501 tatataaaga acctacatat aactttacag cagctactat agttgattca agtgtttgct
     1561 ttattgagaa aaaaatattt gttgaaattc tcgcaaacaa ttctgcattt gcaaatcaaa
     1621 ttattggaaa attatgtaga agttcaaaac atttttttga taaaattacc agtttaggga
     1681 acaaacaatt acatggtcga tttgccgatg cattattata tttgagtaaa aaagttgcaa
     1741 aaagtccaac tattgactta tcaattacac gaaaagaaat tggcgaattg gccggaattt
     1801 ctacagaaag cgccattcgc ttactttcag aattaaatca tgatggtatc attaaattag
     1861 aaggaaaagt aataatcatt ttaaaagaaa atttacttga aaaattgagt caaataggtt
     1921 gatatggatt ctatactttc attagagcca caaattgtgt ggaaacattt ttccgacatt
     1981 tgttctattc ctcacccatc aaaacacgaa gatgttctat tagcgcatat tatttctttt
     2041 gctaaacaac acaacctacc ctacaaacaa gattctatag gaaatatatg cattacaaaa
     2101 cctgctacta aagggcatga acatacagaa acagtttgtt tacaagccca tgttgat
//
LOCUS       DAOTOW010000395         2148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_39919_length_2148_cov_3.579073, whole genome shotgun sequence.
ACCESSION   DAOTOW010000395 DAOTOW010000000
VERSION     DAOTOW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39919_length_2148_cov_3.579073"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="P5243_07390"
     CDS             complement(<1..1470)
                     /locus_tag="P5243_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRS19311.1"
                     /translation="MKNVLRLQISVLLLALLTVRCTNSPKETVPQITGKYSIPPCPEW
                     AKNAVFYQIYPQTFYDTNGDGIGDLQGIIAKLDYIKSLGVDAIWINPFFESPFCDAGY
                     DISDYCKVAPRYGTNEDAKRLFEEAHKRGLKILFDFVASYTSIEHPWFKESAKQEKNK
                     YSNWYIWNDNTWMNPPKEYQDAFIKGYSQRNGQFMRNFYYCQPALNYGFALPDSAYPW
                     QFPTNHPDVMSMREEMKKVVRFWLDMGADGFRADMAGALAKTANIQGNDQFFNTHEDA
                     TKKFWQEIYTMLKHDYPNAYMISEWSYPKDALDGCFHADFYHWFNGYNDLFQKESWRI
                     LNGYSEGHSYFDIEGKGDITHFLESYMDQYTATKDKGFISLPLGNHDNARLANKRSDD
                     DLEIIYAFGLTMPGVPFIYYGNEIGMRQLPETWPQVEGAYKPRNGARTPMQWSNTTNL
                     GFSTASAQQLYLPVDTAKNAPTVAAQEADPRSLLNRTRRL"
     gene            complement(1574..>2148)
                     /locus_tag="P5243_07395"
     CDS             complement(1574..>2148)
                     /locus_tag="P5243_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19312.1"
                     /translation="TTVSSEFFLQQLQAYLPSNISVQSIELVHDRFHARHHARYRSYI
                     YCISKQRTALFKRYVWWVQQYLNSEQMMQAAELFKGFHDFSSFAKTTKKDESTTVEIT
                     HLQIHQVNNCLWIHIVGSHFLWSMVRRIVGVLVEAGKGTVTQQQITQMLTQYSDFPAQ
                     HTAPASGLFLERVYYDNEIIDKSPLFPFTI"
BASE COUNT          674 a          410 c          335 g          729 t
ORIGIN      
        1 cagtcgtcga gttctattga gtaaagaacg agggtcagct tcctgtgcag caacagtagg
       61 tgcatttttt gctgtatcta caggcaaata taattgttga gctgatgctg ttgaaaagcc
      121 taaatttgta gtattactcc attgcattgg cgtacgagct ccatttcttg gtttataggc
      181 accttctact tgcggccacg tttctggcaa ttgcctcatg cctatttcat taccataata
      241 tataaatggc acaccgggca tagtcaaacc aaacgcataa attatttcaa gatcatcatc
      301 gctacgttta tttgccaaac gtgcattatc atgatttcca aggggtaaac ttataaatcc
      361 tttatctttg gttgctgtat attgatccat atatgattca agaaaatgtg taatatcacc
      421 ttttccttca atatcaaagt aactatgtcc ttcagaataa ccgtttaata ttctccaact
      481 ttctttttgg aataaatcat tatatccatt gaaccaatga taaaaatcgg catgaaaaca
      541 tccgtcaaga gcatctttgg gatacgacca ttctgatatc atataagcat tgggataatc
      601 atgtttgagc atagtatata tttcttgcca gaattttttt gttgcatctt catgggtatt
      661 gaaaaattga tcatttcctt gaatattagc cgtttttgca agagcccctg ccatatcggc
      721 tctaaacccg tctgcaccca tatcaagcca aaatcgaact acttttttca tttcttcacg
      781 catactcatt acatcgggat gattcgtggg aaactgccac ggataggcag aatcaggcaa
      841 agcaaatcca taatttaatg ccggttgaca ataataaaag tttcgcataa attgtccatt
      901 tctttgacta taccctttta taaaggcatc ttgatattct tttggtggat tcatccacgt
      961 attgtcattc catatatacc aattggaata cttatttttt tcttgtttag cagactcttt
     1021 aaaccatgga tgttcaattg aagtataact ggcaacaaaa tcaaacaata tttttaatcc
     1081 tcgtttatga gcctcctcaa acaatctttt tgcatcttca tttgttccgt atcgcggtgc
     1141 tactttgcaa taatcagaaa tatcataccc tgcatcgcaa aatggcgatt caaaaaatgg
     1201 atttatccaa atagcatcta ctcctaaact tttaatataa tcaagttttg ctattatacc
     1261 ttgcaaatct cctattccat caccatttgt atcataaaat gtttgcggat atatttgata
     1321 aaaaaccgca ttttttgccc attcgggaca aggtggaata ctatattttc ctgtaatttg
     1381 gggcacagtt tctttaggag aatttgtaca ccgcactgtc aataatgcaa gtaataatac
     1441 cgaaatttgt aatcgtaata cattcttcat ctttgtaaat atttaggtta atttggccta
     1501 aacatacaaa aaaaattaga aaaaaaaagt ataaaacgca tttcttagtg taaataatac
     1561 aacaaaacgc tttctaaata gtaaacggga atagtggcga cttgtctatt atctcattat
     1621 cgtagtatac ccgctcaaga aacaatcccg aagcaggagc tgtgtgttgt gccggaaaat
     1681 cactatattg tgtaagcatt tgagtaattt gttgttgtgt tacagttcct tttcctgctt
     1741 ctaccaatac tcctactatt cgtctaacca tactccataa aaaatgtgaa ccaacaatat
     1801 gaatccacaa acaattattg acttggtgta tttgtaaatg tgtaatttca accgttgtag
     1861 actcatcttt tttggtagtt ttggcaaacg acgaaaaatc atgaaatcct ttaaacaatt
     1921 ctgctgcttg catcatctgt tcactattca aatactgctg cacccaccac acatagcgtt
     1981 taaacaaagc cgttcgttgt ttacttatac aatatatgta acttctataa cgagcatgat
     2041 gccgagcatg aaatctatca tgaactaatt ctattgattg tacagaaata tttgaaggta
     2101 aataggcttg taattgttgt aagaaaaatt cagaagatac agttgttt
//
LOCUS       DAOTOW010000396         2145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40010_length_2145_cov_2.509091, whole genome shotgun sequence.
ACCESSION   DAOTOW010000396 DAOTOW010000000
VERSION     DAOTOW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40010_length_2145_cov_2.509091"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="P5243_07400"
     CDS             complement(<1..963)
                     /locus_tag="P5243_07400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRS19313.1"
                     /translation="MAKETYKKFPPQKLEIDYSGKTPPFSEELEETVLGQLMLEDDAI
                     SKVIDDISAETFYNKKHQVIFAAIAKINANLEPVNIQTVIEQLRKDEELDAVGGPYFI
                     AQLTQKVSSAAHIEHHAKLLAQKFIQRELIRVSAQIQEKAFDPATEIDELVDFSETEI
                     FKVAEGNIKKAAAPISIVLEKALENIKYAQENQKQYSGKPSGFTALDALTSGWQKGNM
                     IVIAARPAMGKTAFVLSMLRNMAIDFGLPVAMFSLEMSNEQLVNRMIVSEAELDHYKI
                     KNGKLSQEDWKNIYEKTKRLAAAPIYLDDTPALSIMEFRAKCRRL"
     gene            complement(1049..1834)
                     /locus_tag="P5243_07405"
     CDS             complement(1049..1834)
                     /locus_tag="P5243_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19314.1"
                     /translation="MKSFFATFFFCLFFLFVSQAQNKNGLYWGLRIGAYSANNKQADF
                     YNGNQSEQSNLATLIEQSYYYDKIAEYYNDDFSIYSQTPIMKYNPTVTVGGLLYYFIS
                     TNLAVYTQVSLVQLQGLGVFQIQLASPIQGTQLGTNIKNGSIHAKEQRFMLDFGLQHT
                     FENKKTYKPYIMGGMSILSMQVIEHTFSIANVEGTFSYYNAQATGNSYFSTFAYGPQI
                     GLGIQVPVQKRTVYIGGEFDYISFQHQNDGFTLQSKLICKIEM"
     gene            complement(1834..>2145)
                     /locus_tag="P5243_07410"
     CDS             complement(1834..>2145)
                     /locus_tag="P5243_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19315.1"
                     /translation="THYMPLMYGTFRFIKVEKQILIYKRKYFEEFAYVVFNKTNSKQT
                     ICFDDFDCNVSILHSMLGNTCNVNKTKITIDLESMSYDIVFSHKHIIETVKLSSDYYI
                     A"
BASE COUNT          694 a          325 c          307 g          819 t
ORIGIN      
        1 aagtcgtcgg cattttgctc taaattccat aattgataat gccggagtat catctaaata
       61 tattggagct gctgctaaac gtttggtttt ttcgtaaata tttttccaat cttcttgtga
      121 taattttcca ttttttattt tgtaatgatc aagttctgct tcacttacta tcattctatt
      181 tactaactgt tcgttactca tttcaagaga aaacatagct accggtaatc caaaatctat
      241 tgccatatta cgaagcatag ataatacaaa cgcggttttt cccattgcgg gacgagcagc
      301 tataactatc atattacctt tttgccaacc tgatgttaat gcatcaagag ctgtaaatcc
      361 ggaaggttta ccactatatt gtttttgatt ttcttgagca tatttaatat tttcgagtgc
      421 cttttctaag actatagaaa taggagcggc agctttctta atattacctt cggctacttt
      481 aaatatttca gtttcagaaa aatcaactaa ttcgtctatt tctgtagctg gatcaaaggc
      541 tttttcttga atttgagctg aaacacgaat taattctctt tgaataaact tttgtgctaa
      601 tagttttgca tgatgttcaa tatgagctgc cgacgatact ttttgtgtta attgagcaat
      661 aaaatatggc ccacctacag catcaagttc ttcgtctttt cgtaattgtt caattacagt
      721 ttgaatattt accggttcta aatttgcatt aattttggca attgcagcaa aaataacttg
      781 atgtttttta ttgtaaaatg tttctgcact aatatcatca attacttttg atatagcatc
      841 gtcttctagc attaattgtc ctaatactgt ttcttcaagt tcctcagaaa atggaggagt
      901 tttacccgaa taatctatct caagtttttg aggtggaaat tttttataag tttcttttgc
      961 cattgttata tatttttaca aacatacaaa atacatggcg ttttttaaac agaaagatac
     1021 aaacaagtat gtgtttgtaa tatgttaatt acatttctat tttacaaatt aacttacttt
     1081 gtaaggtaaa accatcattt tgatgttgaa atgaaatata atcaaattct cctccaatat
     1141 ataccgttcg tttttgaacc ggtacttgaa tgcctaatcc tatttgtggt ccgtaagcaa
     1201 atgtagaaaa ataagaatta cctgttgctt gagcattata atatgaaaac gtaccttcaa
     1261 catttgcaat tgaaaaggta tgttctatta cttgcattga tagaatagac atgcctccca
     1321 tgatatatgg cttatatgtt tttttatttt cgaatgtatg ttgtaagcca aaatcgagca
     1381 taaatcgttg ttcttttgca tgaattgaac cattttttat gttagttccc aattgtgtac
     1441 cttgaatggg acttgctaat tgaatttgaa aaacacctaa tccttgtaat tgaaccaatg
     1501 atacttgtgt atatacagct aaattagttg atataaaata ataaagtaat ccaccaactg
     1561 ttacggtagg attatatttc ataataggtg tttgtgaata tatactgaaa tcatcattgt
     1621 aatattctgc aattttatca taataatatg attgttcaat aagagttgct aaattacttt
     1681 gttctgattg gttgccatta taaaaatcag cttgtttatt atttgcacta tatgctccaa
     1741 ttcttaatcc ccaatataag ccgtttttat tttgtgcttg tgaaacaaac aaaaaaaaca
     1801 aacaaaaaaa gaatgttgca aaaaaagatt tcattatgct atataataat ctgaagaaag
     1861 ttttacagtt tcaataatat gtttatgtga aaatactata tcataactca tagattctaa
     1921 atcaatagta atttttgttt tattcacatt acaagtattt cccaacatgg aatgtaatat
     1981 tgatacattg caatcaaaat cgtcaaaaca tatagtttgt ttgctattag ttttattaaa
     2041 tacaacatag gcaaattctt cgaaatattt gcgtttatat atcaatattt gtttttctac
     2101 ttttataaaa cgaaatgtac catacatgag aggcatgtag tgagt
//
LOCUS       DAOTOW010000397         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40029_length_2144_cov_2.730493, whole genome shotgun sequence.
ACCESSION   DAOTOW010000397 DAOTOW010000000
VERSION     DAOTOW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40029_length_2144_cov_2.730493"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="P5243_07415"
     CDS             <1..517
                     /locus_tag="P5243_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS19316.1"
                     /translation="AVYFPANLISLTMLNTVAFDFWPVQPIIAMRTPIIRQIAMATLH
                     FGIFEIIVKRGLYYKERCTKELMDLFWKPMKTSIGRKAFLQFAHCLNNSNLTEIEREI
                     SEIQVPVLIIRGEADVYLASIISESLHAHISHSTYVVIPTAGHFIQEDEPKQVADLIL
                     QHIQKYNRHFI"
     gene            525..>2144
                     /locus_tag="P5243_07420"
     CDS             525..>2144
                     /locus_tag="P5243_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS19317.1"
                     /translation="MKSINTTIQELDSKIQILERENETLSSMAEVNLLLYNAFIEIDT
                     TQSIDTLFHTILEHISIIMHLPYTGFFINNQHTITCIQSYALFDEENKLSTISCIDSI
                     AFQNADYTILQQHDFTFVYPNFSIQTNQLLYIPINCQYQQGLVLICISAEDETISPEK
                     ADILYKVTRMLRTKLESLYFQEQLKQSNMLLEQKVSERTHELIEQNNQYSILNNEYKK
                     LNDELYQAKLQAEQNELRYREIFDSSIDAIFIHNTENDTIEDVNNAMLEMYGYNKSEI
                     SVLKIGNLSAEVYGYTSEKALQYIKQSSEQGVVSFEWYAKKKNDELFWVFVVLKPIVI
                     HGIQRIMAIVRDIHQQKLTKEAIRISEEKFAKAFMASPDSIIISSLQDAVYIDVNEAF
                     LQQTEYTREEIIGNSSIELQIWNKASDRDLFVKQLREQGWVRNFEAEYKSKTGKIGLF
                     SISAEIIHINEQLCVLAQCKNITESKRIQQENDYRRQVLDTLMDNLPIGVFMVEAHTG
                     KPIFANNKACELLGRGILPDANEYNISEVYQA"
BASE COUNT          814 a          296 c          359 g          675 t
ORIGIN      
        1 tgctgtttac tttcctgcaa atcttataag tcttaccatg cttaatactg ttgcttttga
       61 tttttggccg gtacaaccaa ttatagctat gcgaactcct ataatccgtc aaatagctat
      121 ggctacgtta catttcggaa tttttgagat aatagtaaaa cgaggattgt actacaaaga
      181 acgttgcaca aaagaactta tggatttgtt ttggaaacct atgaagactt ctatagggcg
      241 taaggcattt ttacaatttg ctcactgttt aaataattca aatttaaccg aaattgagag
      301 agaaatatct gaaatacaag ttccggtact tattattcgt ggtgaagccg atgtgtattt
      361 agcttcaata atttcggaga gtttacatgc tcatatttca catagtacgt atgttgtaat
      421 tcctactgca gggcatttta ttcaagaaga tgaaccaaaa caagttgcag atttaatttt
      481 acaacatatt caaaaatata ataggcattt tatatagaat aacaatgaaa agcattaata
      541 caaccataca agaattagat tcaaaaattc aaattcttga acgagaaaat gaaacacttt
      601 catcaatggc agaagtgaat ttattgttgt ataatgcgtt tattgaaata gatactacac
      661 agtctataga cacgttgttt cacacaatat tggagcatat ttctattatt atgcatttac
      721 catataccgg attttttata aacaaccaac atacaattac gtgtattcaa agttatgcct
      781 tgtttgatga agaaaataaa ttaagtacaa tttcatgtat tgatagtata gcttttcaga
      841 atgcagacta taccatatta caacaacacg attttacatt tgtatatcca aacttttcaa
      901 tacaaacaaa tcaattgttg tatataccaa ttaattgtca ataccaacaa ggtttggttc
      961 ttatttgtat ttctgcagaa gatgaaacaa tatcacctga aaaagccgat atattatata
     1021 aagtaactcg aatgttacga accaaactgg aaagtttata ttttcaggaa caattaaagc
     1081 aatcaaatat gcttcttgaa caaaaagttt ctgaacgaac acatgaactg attgagcaaa
     1141 ataatcaata tagtattctc aacaatgaat ataaaaagct taatgatgaa ttatatcaag
     1201 caaaattgca agcagaacag aatgagttgc gatatcgaga aatttttgat tcatctattg
     1261 atgctatttt tatacataat accgaaaatg atacaataga agatgtaaac aatgcaatgc
     1321 ttgaaatgta tggttacaat aaaagcgaaa tatcagtttt aaaaataggt aatttaagtg
     1381 ccgaagtcta tgggtatact tctgaaaagg ctttgcaata tattaagcaa tcttcagagc
     1441 aaggggtagt gtcatttgaa tggtatgcaa aaaagaaaaa tgatgaacta ttctgggtat
     1501 ttgtagtttt aaaacctatt gtaatacatg gtatacagcg cataatggct atagtgcgag
     1561 atatacatca acaaaaatta acaaaagaag caattagaat ttcggaagaa aagtttgcca
     1621 aagcatttat ggcaagtcct gattctataa ttataagttc tttacaagat gcagtgtata
     1681 ttgatgtaaa tgaggcattt ttgcaacaaa cagaatatac acgtgaagaa ataatcggga
     1741 actcatcgat tgaattgcaa atttggaata aagccagtga tagggatctt tttgtaaaac
     1801 aacttcgaga acaggggtgg gtgcgaaatt ttgaagcaga atataaatca aaaacaggta
     1861 aaatagggtt gttttctatt tcggcagaaa taatacatat aaatgaacaa ttgtgtgtgt
     1921 tggcacaatg taaaaatatt accgaaagta aacgtattca acaagaaaat gattatagac
     1981 gtcaagtatt agatacgctt atggataatt tacctatagg agtatttatg gttgaagctc
     2041 atacaggaaa acctattttt gcaaataata aagcatgtga attattaggt agaggaattt
     2101 tacccgatgc aaacgaatac aatatttcgg aagtgtatca agca
//
LOCUS       DAOTOW010000398         2136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40208_length_2136_cov_3.753484, whole genome shotgun sequence.
ACCESSION   DAOTOW010000398 DAOTOW010000000
VERSION     DAOTOW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40208_length_2136_cov_3.753484"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..501)
                     /gene="rplL"
                     /locus_tag="P5243_07425"
     CDS             complement(118..501)
                     /gene="rplL"
                     /locus_tag="P5243_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRS19318.1"
                     /translation="MADLKKLAEELVNLSVKDVKELADILKQEYGIEPAAAAAVAVAA
                     PAAGGGAAAAAAEQTEFDVIMKSAGAAKLQVVKVVKDITGLGLKEAKDIVDGAPKAVK
                     EKVSKEEAENIKKQLEEAGAEVELK"
     gene            complement(545..1069)
                     /gene="rplJ"
                     /locus_tag="P5243_07430"
     CDS             complement(545..1069)
                     /gene="rplJ"
                     /locus_tag="P5243_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRS19319.1"
                     /translation="MDIAQKRDIVESIVQKINENGHFYITDIEALNAEDTSNLRRKCF
                     ENDIEMVVVKNTLFKKALEQIGGNDYQDLYGVLKGTSSVLFCKTANIPAKLIKEFRNE
                     GKQKPVLKGAFAEQCIYIGDDQLDALVSIKSKEELIGDIILLLQSPIQQLIGQLNSGK
                     NTIAGIVKTLSEKE"
     gene            complement(1096..1788)
                     /gene="rplA"
                     /locus_tag="P5243_07435"
     CDS             complement(1096..1788)
                     /gene="rplA"
                     /locus_tag="P5243_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRS19320.1"
                     /translation="MMKLTKNRKSIIGKIEPNKAYTIEEAAKLLKDITTTKFDASVDL
                     DVRLGVDPRKANQMVRGVVTLPHGTGKEVRVLVLCTPDKEKEAKEAGADYVGLDEYID
                     KIKGGWTDIDVIITMPTVMAKVGALGRVLGPRGLMPNPKSGTVTMEIGKAVAEVKSGK
                     IDFKVDKYGIVHSSIGKASFAPEKIVDNAQEFVSIINKLKPSSAKGTYIKSIYISTTM
                     SPGILVDTKSFV"
     gene            complement(1800..>2136)
                     /gene="rplK"
                     /locus_tag="P5243_07440"
     CDS             complement(1800..>2136)
                     /gene="rplK"
                     /locus_tag="P5243_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRS19321.1"
                     /translation="MEFCKQFNARTQDQAGKVLPVIITVYSDKSFTFIIKTPPVAIQL
                     MEIAKLKGGSAEPNRKKVASVTWAQVKAIAEAKMPDLNCFTIESGMKMVAGTARSMGI
                     TIEGEMPSV"
BASE COUNT          585 a          415 c          291 g          845 t
ORIGIN      
        1 tctctccttg gttttcattg caaaaatata aaatttgaac ttaaaatatg taaaatgaga
       61 aaaggtttaa gtcatgttgc catgacttaa accctatttt tttaagaaat ccagttctta
      121 tttaagttca acttctgctc cggcttcttc taattgtttt ttaatatttt cagcttcttc
      181 tttagaaact ttttctttca ctgctttagg agcaccgtca actatgtctt ttgcttcttt
      241 aagaccaaga cctgtgatgt cttttacaac ttttacaact tgtaattttg cagcacctgc
      301 tgatttcata attacatcaa actcagtttg ttctgctgct gccgccgctg cgcctccacc
      361 tgctgcaggt gctgctacag ctacagctgc tgctgctgcc ggttcaatac catattcttg
      421 tttaagaata tctgctaatt cttttacgtc ttttacgctt agatttacta attcttctgc
      481 taattttttt aaatctgcca tgtctttgaa tgatttaatt gtactgtact ttattattat
      541 ttaattattc cttttcagat aatgttttta cgatacctgc aattgtattt tttcctgaat
      601 taagctgtcc aataagctgt tgaataggag actgaagaag taatataata tctccaataa
      661 gttcctcttt cgattttatt gaaactaatg catctaattg gtcatcacct atgtaaatac
      721 attgctcagc aaatgctcct tttaatacag gtttttgttt gccttcattt ctaaactctt
      781 tgataagttt agcaggtata tttgcagttt tacaaaataa aactgatgaa gttcctttta
      841 aaacaccata taaatcttgg taatcattgc caccaatttg ttctagtgct tttttaaata
      901 gagtgttttt tacaacaacc atttcaatat cgttttcaaa acactttctt cgtaaattac
      961 ttgtatcttc ggcatttaat gcttcaatat cagtaatgta gaaatgtccg ttttcgttaa
     1021 ttttttgaac tatactttct actatatctc gtttttgtgc tatatccata atgttatact
     1081 cctgttttca aaaaattaaa caaatgattt cgtatctaca agaatacccg gactcatagt
     1141 agttgaaata taaatacttt taatataagt tccttttgct gatgaaggtt ttaatttatt
     1201 aataatgctt acaaattctt gtgcattatc aacaattttt tcaggagcaa acgatgcttt
     1261 tccaattgat gaatgaacaa taccgtattt atcaacttta aaatcaattt ttccggattt
     1321 tacttctgct acagctttgc caatttccat ggttactgta ccacttttag ggttaggcat
     1381 taaaccacgt ggtcccaaaa ctctaccaag agctcctact tttgccataa ctgtaggcat
     1441 agtaataatt acatctatat ctgtccaacc acctttaatt ttatctatat attcgtcaag
     1501 ccctacataa tctgctcctg cttcttttgc ttctttttct ttatcaggag tacacaatac
     1561 taatactcga acttcttttc cggtaccgtg aggtagggtt acaacgccac gaaccatttg
     1621 atttgcttta cgcggatcta cacctaaacg tacatctaaa tctactgaag catcaaattt
     1681 tgttgtagta atatctttca acaattttgc agcttcttca attgtatatg ctttattagg
     1741 ttctatcttg ccaattatac tttttctgtt ttttgtaagc ttcatgattc caataatttt
     1801 taaactgaag gcatttctcc ttcaatagtt atacccatgc tgcgagctgt tccagctacc
     1861 attttcattc ccgattcaat tgtaaaacaa ttcaaatccg gcattttagc ttcagctatt
     1921 gcttttacct gtgcccatgt tacgctggca acttttttac ggtttggctc agccgatcct
     1981 ccttttaatt ttgcaatttc cattaattga attgctaccg gaggagtttt gatgatgaat
     2041 gtaaatgatt tgtcggaata tacggtaata attaccggta atacttttcc tgcttgatct
     2101 tgagttcttg cattgaattg cttacaaaat tccatt
//
LOCUS       DAOTOW010000399         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40391_length_2129_cov_2.608004, whole genome shotgun sequence.
ACCESSION   DAOTOW010000399 DAOTOW010000000
VERSION     DAOTOW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40391_length_2129_cov_2.608004"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..728
                     /locus_tag="P5243_07445"
     CDS             318..728
                     /locus_tag="P5243_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRS19322.1"
                     /translation="MVRPKQKRTVTKPPIMEGFKPFGIPMTEMEPLVLLYEEYESIRL
                     TDYMKYTQEEAAVHMKVSRPTFTRIYENARQTIAKAFVEGKAIFIEGGEYHSDDYWYR
                     CDSCKMVHVSESIMFNCMSCNSNNIRLLNNKIGG"
     gene            730..903
                     /locus_tag="P5243_07450"
     CDS             730..903
                     /locus_tag="P5243_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19323.1"
                     /translation="MDCSEQAYCICVHCNIVVAHTKGIPCKEVVCEKCGKRMIRQGGY
                     HHTLYLKKQTKEK"
     gene            924..1343
                     /locus_tag="P5243_07455"
     CDS             924..1343
                     /locus_tag="P5243_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRS19324.1"
                     /translation="MKIAVPTRQNYIDDHFGHCEMYTIYTVDEKTILEVETIPSTQGC
                     GCKSDIAKVLQNNGVTVLLAGNMGAGALQVLQKHGITVLRGNSGEVTSVVHDYLQGKL
                     TDSGIGCASHESHHGHGHNGGHNCSHSHTNTEFSIDI"
     gene            1353..1538
                     /locus_tag="P5243_07460"
     CDS             1353..1538
                     /locus_tag="P5243_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19325.1"
                     /translation="MTKLNMQGPENVKHIQGRQLGICDNHSSNYTLGKGMGLRRKSGG
                     GTGMAKRLQSGQNYDRT"
     gene            1525..1929
                     /locus_tag="P5243_07465"
     CDS             1525..1929
                     /locus_tag="P5243_07465"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRS19326.1"
                     /translation="MIEHKYSLRVQYPDTDKMGTVHHSNYAKYYETARWELFRSIGIS
                     YSSIEQAGYLLPVIEMKTKFYKTTYYDAQLTVITKIVGMKGPRIFFESTLYNQENELL
                     NSCDIELAFVKKDTWKPCSAPLFVLEILQKYV"
     gene            1922..>2129
                     /locus_tag="P5243_07470"
     CDS             1922..>2129
                     /locus_tag="P5243_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRS19327.1"
                     /translation="MSKSCIFVTIMNTDKQYYIDKALDLFNQGYACSQAVLMAFASDF
                     AIDETTAKRIASTFGGGMGRLREKC"
BASE COUNT          796 a          253 c          416 g          664 t
ORIGIN      
        1 aggaaatttt gtaaaacaag attttttacc atcagatatt attggaattt ctttttggaa
       61 accgggaact aatcaacgag aagaagaatt aaaaaaatat atgcaaaaaa tctggggaaa
      121 ttactatacc ttctaatagt tttctttttc tacttatttt atttaccctc aattgtttaa
      181 atgtttttaa cagaagttaa atttatatta ttttttagta tctattaaaa aattattaat
      241 tgtataaaat atttattata attatgaaca tatgttcata attatatata tatttgtatt
      301 tattgtattg agaaaaaatg gtgagaccca aacaaaaaag aacagtaaca aagcctccga
      361 ttatggaagg tttcaagcct tttggaatac ctatgaccga gatggagcct ttagtgttat
      421 tgtatgaaga atatgagtct attcgattga cagattatat gaaatataca caagaagaag
      481 ctgcggtaca catgaaagtg tcacgaccaa cgtttaccag aatttatgaa aacgcacgac
      541 aaactattgc aaaagctttt gttgaaggta aagcaatatt tatagaaggt ggcgaatatc
      601 attccgatga ttattggtat aggtgtgatt cgtgtaaaat ggttcatgta agcgagagta
      661 ttatgtttaa ttgcatgagt tgtaacagta ataatataag attgttaaac aataaaatag
      721 gagggtaata tggattgtag tgagcaggct tattgtatat gtgtgcattg taatattgta
      781 gtggcacata ctaaaggaat accatgcaaa gaagttgtgt gtgagaagtg tggtaaacgt
      841 atgattagac aaggaggata tcatcataca ttatatttaa aaaaacaaac aaaagaaaaa
      901 taacaatact aatttttaaa aatatgaaaa tagcagttcc aacaagacaa aattatattg
      961 atgatcattt tggtcattgt gaaatgtata caatatatac ggtagatgaa aaaacaatac
     1021 tcgaagtaga aacaattcca tcaactcaag ggtgtggatg caaaagtgat attgcaaaag
     1081 ttttacaaaa caatggagtt acagtattac tggccggtaa tatgggagca ggagcattgc
     1141 aggtgttgca aaaacatggt attacggtat tgcgaggtaa ttccggtgaa gttacgagtg
     1201 ttgtacatga ttatttgcaa ggaaaattaa cagattccgg gattggatgt gcaagtcatg
     1261 agtcgcatca tggtcatgga cataatggcg gacataactg tagtcattca catacaaata
     1321 ctgagttttc gattgatata tagagaaatg atatgacgaa attaaatatg caaggtcctg
     1381 aaaatgtaaa acatatacag ggccggcaat tgggtatatg tgataatcat tcctcaaatt
     1441 atacattggg aaaaggaatg gggcttcgcc gaaaatcggg tggtggcaca ggtatggcaa
     1501 aacggttaca aagtggacag aactatgata gaacataaat attcattgag agtgcaatat
     1561 cctgatacag ataaaatggg aactgtacat cattcaaatt atgctaaata ttatgaaacc
     1621 gctcgctggg aattgtttag aagtattggt atttcttaca gttcaataga acaagcagga
     1681 tatttgttgc ctgtaataga aatgaaaact aaattttata agacaacata ttatgatgct
     1741 caattaacag taataacaaa aatagttgga atgaaaggtc ctcgtatatt ttttgaaagt
     1801 acattgtata atcaagaaaa tgaattacta aattcttgtg atattgaact ggcatttgtt
     1861 aaaaaagata catggaaacc ttgtagtgca cctttatttg ttttggaaat attacaaaaa
     1921 tatgtctaaa tcatgtatat ttgtaacaat tatgaatact gataaacaat attatattga
     1981 taaagcatta gatttattta atcaaggata tgcgtgttcg caagctgtat tgatggcgtt
     2041 tgcttctgat tttgcaatag atgaaacaac agcaaagcgt atagcatcta catttggcgg
     2101 cggaatggga aggttgcgcg aaaaatgtg
//
LOCUS       DAOTOW010000400         2126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40476_length_2126_cov_3.029454, whole genome shotgun sequence.
ACCESSION   DAOTOW010000400 DAOTOW010000000
VERSION     DAOTOW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40476_length_2126_cov_3.029454"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="P5243_07475"
     CDS             <1..531
                     /locus_tag="P5243_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19328.1"
                     /translation="FPVIMIVMGVTIVISISELGDVAEGKYATVDSMLEHAVIIQQDL
                     TRTEAYGENTFDIGKFDASIPSMLSKAPVAIIAGMFRPFIWEARSILMIFSGFESAFL
                     MGFFLYLLFRTGIVGFFALVQKDAFLISSIAFAVMFGFFVGLTTANFGALVRYKIALI
                     PFFTIVLFRIYGLIKT"
     gene            complement(539..>2126)
                     /gene="cysC"
                     /locus_tag="P5243_07480"
     CDS             complement(539..>2126)
                     /gene="cysC"
                     /locus_tag="P5243_07480"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRS19329.1"
                     /translation="HEQYTRNMVTGASTANLAIVLVDATKGVITQTRRHTFLVSLLGI
                     KHVVLAVNKMDLVDFSEQRFNEICSQYKQFVSQLDIPDVQYIPLSALKGDNVVETSER
                     LPWYEGKSMLHFLETVHISSDRNFEDLRYPVQFVSRPDRDFRGFCAKIASGIIKKGDT
                     IMVLPSRKTSTITSIVTYDGELEQAFPPQSVTITLAHEIDISRGEMLVHPDNLPHIER
                     QFEAMLVWMDEKPMNPNTQFYIKHNTNTTKARFDTIRYKVDVNTLEKSDITHLELNEI
                     GRVVITTVKPLFFDAYKKNRNTGSFVLIDPVSHNTCAVGMIIDKIHADGLPQRITDAD
                     KQKIDTGIGLISQQEYENRYNQKGATIWITGLHGSGKNQLAYSLERQLFNQDAIVVLF
                     DGSTVRSGLSKELDYSPADRAENLRRVAEVCKVLNDQGIITICSFISADDSVRKQVSS
                     IIGAERFTLVYMDADLDYCKNNKPELYSLIEQGKIDNLPGIDCAYDIPTSADIVCKPH
                     DLEDNVQKIIHYLIEKGIKK"
BASE COUNT          622 a          342 c          376 g          786 t
ORIGIN      
        1 tttcctgtaa ttatgattgt aatgggagtt accatagtaa tttctatatc agaattagga
       61 gatgtggcag aaggcaagta tgcaacggta gattctatgt tggaacatgc ggtaattatt
      121 caacaagatt taactagaac cgaagcatac ggcgaaaaca cgtttgatat aggtaaattt
      181 gatgcttcta tacctagtat gttgtcaaaa gctccggtag ctataattgc cggaatgttt
      241 cgtccattta tatgggaagc acggagtatc cttatgatat tttcaggttt tgagagtgct
      301 tttttaatgg gtttctttct ctatttatta tttagaacag gtattgttgg tttttttgca
      361 ttagtacaaa aggatgcatt cttaatatca agtatagcat ttgccgtaat gtttgggttc
      421 tttgtaggac ttactactgc aaattttggt gcgttggtac gatataaaat tgctctaatt
      481 ccttttttta ctatagtact tttccgtatt tatggtttaa taaagacgta ggtacatatt
      541 attttttaat acctttttct attaaataat gtattatttt ttgtacatta tcttctaaat
      601 catgtggttt acatacaata tcggctgatg ttggaatatc atatgcacaa tcaataccgg
      661 gtaaattatc aattttccct tgttctatta atgagtataa ttcaggtttg ttatttttac
      721 aatagtctaa atctgcatcc atatacacta atgtaaaccg ttctgcacca ataattgaac
      781 ttacttgttt tcgaacactg tcatcggcag aaatgaaaga acaaatagtt ataattccct
      841 ggtcgtttaa cactttacat acttcggcaa ctcggcgtaa attttcagct ctgtcggcag
      901 gtgagtagtc aagttctttt gacaaaccgc ttcttactgt gctaccatca aataatacaa
      961 ctatagcgtc ttgattaaat aattgtcttt caagagaata tgctaattga tttttgcctg
     1021 aaccatgtaa tccggtaatc caaatagttg cacctttttg attataacgg ttttcgtatt
     1081 cttgttgtga aatgagccca ataccggtat caattttttg tttgtcggca tctgttatac
     1141 gttgtggtaa tccgtcggca tgaattttat ctattatcat accaactgcg caagtattgt
     1201 gacttacagg gtctattaat acaaatgaac cggtgtttcg attttttttg taagcatcaa
     1261 aaaataaagg ttttacggta gttataacaa ctcgtccaat ttcatttaat tctaaatgtg
     1321 taatatctga tttttcaaga gtatttacat ctactttgta acgaattgta tcaaatcgag
     1381 cttttgtagt atttgtatta tgttttatat agaattgggt gtttggattc attggttttt
     1441 catccatcca taccagcatt gcttcaaatt ggcgttcaat gtggggtaaa ttatcgggat
     1501 gaactaacat ttcgcctcgt gaaatatcaa tttcgtgggc aagagtaatt gttacggact
     1561 ggggtgggaa tgcttgttcc aattctccat cataggtaac aatagatgtt atggtagatg
     1621 ttttacgcga aggtaaaacc attattgtat ctcctttttt tataataccc gaagctattt
     1681 ttgcacaaaa acccctaaag tcacggtccg gtcttgatac aaattgtaca ggatatcgta
     1741 aatcttcaaa atttctgtcg ctgcttatat gtacagtttc taaaaaatga agcatacttt
     1801 taccttcata ccaaggtaac cgttctgatg tttcaaccac attatcgcct ttgagtgcag
     1861 atagaggaat gtattgaaca tctggtatat caagttgtga cacgaattgt ttgtattgac
     1921 tacaaatttc attaaaacgt tgttcactaa agtcaactaa atccatttta ttaacagcca
     1981 ataccacatg ttttatacct aataatgata ctaaaaatgt atgtcggcga gtttgtgtaa
     2041 ttacaccttt ggtagcatct actaatacta tggcaagatt tgcggtagaa gctccggtaa
     2101 ccatgttgcg tgtatattgt tcatga
//
LOCUS       DAOTOW010000401         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40498_length_2125_cov_3.007729, whole genome shotgun sequence.
ACCESSION   DAOTOW010000401 DAOTOW010000000
VERSION     DAOTOW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40498_length_2125_cov_3.007729"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="P5243_07485"
     CDS             <1..313
                     /locus_tag="P5243_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS19330.1"
                     /translation="GLLTDRYIAGIPQDSRAAKSHGFLQSNDVNDAVIQKVIQLQAVA
                     KKRNQSLAEMAIAWIWEKGITSVLIGASSVAQLQTNLQALHSQPFSQEHIQEIENILE
                     L"
     gene            360..1457
                     /locus_tag="P5243_07490"
     CDS             360..1457
                     /locus_tag="P5243_07490"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HRS19331.1"
                     /translation="MIDVIVDILKNSLSITFFVLTTMLFIEYINVRSSGTFNSIIQKN
                     GLRQIILGAFLGVIPGCLGTYTMVTFYTHNIVTIGALTATFIATMGDEAFLLFSIQPL
                     QAIGVFVVLFIIGIFTGVIVDLLYTKKKADTLQVAHFEIHETEQTFQKASFSLIINNI
                     KHASPHRALLMFGIAVIFILSLLGQIGHSHDFLSSFAIQHASVEHIHNHDHEINWLAV
                     TFVLLSIGAAYIVSSVNEHFLEEHFWSHIIQKHFIKIFSWTTLVLVIVTMLNTYIHIE
                     DFLQTYTYHILFLAVLVGIIPESGPHIVFLSLYVSGTIPLSILMANSIVQDGHGALPL
                     IAESKKTFLQVKFINIVVGLLVGIIGLQLGI"
     gene            complement(1460..>2125)
                     /locus_tag="P5243_07495"
     CDS             complement(1460..>2125)
                     /locus_tag="P5243_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS19332.1"
                     /translation="DVEYTFYLLSTPSAYNKNFTNTVSRILGAKEYFKKIQTTTGEKP
                     HIPGIQILDKYTLKITLETPSVLFLYNLAHPAASILPQEGIKKYDSDCKIGAGPFIYD
                     WQRDSQYIVLTRNKHFFMFDKADMRLPYLDTILLIKTKNQQHSIDLFVNNQTDALLYV
                     ETDKIQDILNLKKDSIQYKLTKSYANLEGNNTLYNIYSESIHDFYTNNLHILDLTKVF
                     IKK"
BASE COUNT          699 a          272 c          342 g          812 t
ORIGIN      
        1 agggttatta acagaccgct atattgcagg tattcctcaa gattcacgag cagctaaatc
       61 tcatggtttt ttacaatcaa atgatgtaaa tgatgctgtt attcaaaaag taatacaatt
      121 acaggctgtt gcaaaaaaac gtaatcaaag tctagcagaa atggctatag cgtggatttg
      181 ggaaaagggt ataacatctg tactaattgg agcaagttct gttgctcaat tgcaaacaaa
      241 tttacaagcg ttgcattcac aaccattttc tcaagaacat attcaagaaa ttgaaaatat
      301 cttagaatta tagagtaagt tataatgtat atgttagtct atacataaaa aatgaaacta
      361 tgatagatgt aattgttgat attttaaaaa actctttgtc aatcactttt tttgtgttga
      421 ctacaatgtt atttattgaa tatattaatg ttcgttcgtc gggaacattt aattcaataa
      481 tacaaaaaaa tggattgagg caaattatat taggtgcttt tttaggagtt attcccggat
      541 gtttaggaac atatactatg gttacttttt atactcataa tattgtaact ataggagcac
      601 ttactgctac atttattgca actatgggcg atgaggcctt tttattattc tctatacaac
      661 ctttacaagc tataggtgtt tttgtagttt tgtttattat tggtattttt acaggcgtta
      721 ttgtagattt gttatataca aaaaaaaagg cagatacatt acaagttgct cattttgaga
      781 ttcacgaaac agagcaaacg tttcaaaaag cttcattttc gcttataatc aataatataa
      841 aacatgcttc acctcataga gctttattaa tgtttggaat cgcagtaatt tttattttat
      901 cgcttctggg ccaaattgga catagtcatg attttttatc atcatttgca atacaacatg
      961 cttctgttga acatattcat aatcatgatc atgaaatcaa ttggcttgct gttacttttg
     1021 tattactatc tatcggtgca gcatatatag taagtagtgt aaatgaacat tttttggagg
     1081 agcatttttg gagtcacatt atacaaaaac attttattaa aatcttttca tggacaacac
     1141 ttgtactagt aatagttacc atgttaaata catatattca tattgaagat tttttacaaa
     1201 cgtataccta tcatatatta tttcttgcag tacttgtagg tattattcct gaatcagggc
     1261 ctcatattgt ttttctttca ttatatgttt ctggcacaat acctttaagt attttaatgg
     1321 caaattcaat tgtacaagat ggtcatggtg cattaccatt aattgctgaa tcaaaaaaga
     1381 cttttttaca agttaaattt ataaatattg ttgtagggtt acttgttgga ataatagggc
     1441 ttcaattggg tatataatat tattttttta taaaaacctt tgtaagatcc aatatatgta
     1501 agttgttggt ataaaaatca tgaattgatt cagaataaat attatataga gtattattcc
     1561 cttctaaatt agcataagat tttgtaagtt tatattgtat agaatctttt tttagattca
     1621 atatatcttg aattttatct gtttctacat ataacaaagc atcggtttgg ttatttacaa
     1681 aaaggtcaat agaatgttgt tggttttttg tttttattaa tagtatagta tcaagatacg
     1741 gaagacgcat gtcggctttg tcaaacataa aaaaatgttt attgcgagtt aagactatat
     1801 attgcgaatc gcgttgccaa tcgtaaataa aaggacctgc tccaatttta cagtctgaat
     1861 catatttttt tattccttct tgaggaagta tagatgcagc agggtgtgct aaattataga
     1921 gaaacaaaac agacggggtt tctaatgtta tttttaatgt atatttatct aatatttgaa
     1981 tgcctgggat atgtggtttt tctcctgtag tagtttgaat ttttttaaaa tattcttttg
     2041 ctccaagtat tctggatacg gtatttgtaa agtttttatt atacgcagaa ggtgtagata
     2101 ataaataaaa tgtgtattct acatc
//
LOCUS       DAOTOW010000402         2122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40577_length_2122_cov_3.454765, whole genome shotgun sequence.
ACCESSION   DAOTOW010000402 DAOTOW010000000
VERSION     DAOTOW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40577_length_2122_cov_3.454765"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2122
                     /locus_tag="P5243_07500"
     CDS             <1..>2122
                     /locus_tag="P5243_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19333.1"
                     /translation="TGKVTFTVRVKTALHPYICLQSTITATNCDSWTSNEYPNNATYT
                     MERNCVDILANRSLIVNKTANRTEMNPANEVNFRVDFENKSTADSWLNGGRDHVILSY
                     GNFIPPGGGYSSTSFYQLFRFWNDAQEAYINMNNYRISYFMNDAAAMGLYNATTNPTG
                     WTFQVDNQNDLDKYGYNPASGPITFAYQKIPNGSDANGAWNQRLMIRFANTLMATSTH
                     VYDKLDSKYLLHKGVWGPALIRAVLSANPTADMIVRTSDDWSYSSAVKSASLDGQGET
                     FTPISPGWANRATPNKPITNYSRHSCSPDRANYDRILVEEFDGYTWRRILGRGPLPGK
                     EAEMVVVVDTIPKEFTWKAFKTDKALGVTATYTPAPNGANYTGIVKWQIPSMLVGEKG
                     FLEYTVIAAGTCPMADKNVVNKAWIYSSTDSPTSSEAPVKITCDFVPEPITPTSITKT
                     ADKTTYAVNDNIVYTIDYVQKQGAIAIDPLTATSTTSNWVAPAGSPGLPTNVGGDLTV
                     PWTDPKYFYNKYSHGTNGTLTFTIDLTTNGWNSFSLLFRYKSGKPGTAGFDGVALNIW
                     PGKNGIGAGLVISCMNNNTLVRRDGETSPLSYSAPATPLTITVRLLDDKMSVWVNSDP
                     ETNTPLVTYSGLKIQAGYVGFYNGKSDSGLSGGGENSNNYRIKKWDSRFDSAFDLEIS
                     DVLPTVTSFVSATNSGTHSAGKVTW"
BASE COUNT          700 a          382 c          421 g          619 t
ORIGIN      
        1 accggtaaag taacatttac ggttcgtgta aaaacggcat tgcatccata tatttgttta
       61 caaagtacca ttactgcaac aaattgcgat agttggacaa gcaatgaata ccccaacaat
      121 gcaacctata caatggaacg aaattgtgtt gatattttgg ctaatcgttc gttaattgta
      181 aataaaactg caaacagaac cgaaatgaat cctgcaaatg aggtaaattt tcgtgttgat
      241 tttgaaaaca aatctactgc cgattcatgg ttaaatggtg gtagagatca tgtaatttta
      301 tcgtatggta attttattcc acccggaggt ggctattcat ctaccagttt ttatcaattg
      361 ttccgtttct ggaacgatgc acaagaagca tacataaaca tgaataacta ccgaatttca
      421 tattttatga atgatgctgc agctatgggt ttatacaatg ccacaacaaa tcctacaggg
      481 tggacatttc aagttgacaa tcaaaatgac ttagataaat atggatataa tcctgcatcg
      541 ggaccaataa catttgctta tcaaaaaatt ccaaatggaa gtgatgcaaa tggagcatgg
      601 aatcaacggt taatgattcg ttttgcaaat acacttatgg caacatctac acatgtgtat
      661 gacaaattag acagtaaata tttgttacac aaaggtgtgt ggggacctgc tttaatcaga
      721 gcagttttaa gtgctaatcc tactgcagat atgatagttc gaacatctga tgactggtca
      781 tattcatctg cagtgaaatc tgcatcactc gatgggcaag gtgaaacatt tactcctatt
      841 tcacccggtt gggcaaacag agcaacaccg aataaaccta taacaaatta ttctcgccat
      901 tcgtgtagtc ccgatcgtgc aaattatgac agaatcttgg ttgaagaatt tgatggatat
      961 acttggcgtc gtattttagg acgtggtccg ctacccggaa aagaagccga aatggtagta
     1021 gtggttgata ctattcctaa agaatttaca tggaaagcat ttaaaaccga taaagcattg
     1081 ggggttaccg caacatatac tcctgcacca aacggagcta attataccgg aattgtaaaa
     1141 tggcaaattc cttcaatgct tgttggtgaa aaaggattct tggaatatac tgtaattgct
     1201 gcaggtactt gtcctatggc cgataaaaat gttgtaaaca aagcatggat atattcatca
     1261 accgattcac ctacatcgag cgaagcaccg gttaaaatta cctgcgattt tgtgccggaa
     1321 cctattacac caacctctat tacaaaaact gccgataaaa ctacctatgc ggtaaatgat
     1381 aatatagtat ataccataga ttatgtacaa aaacaaggtg ctattgccat tgacccatta
     1441 actgcaacgt ctacaacatc taattgggtt gcacctgcag gtagtccggg attaccaaca
     1501 aatgttggcg gcgatttaac agtaccatgg accgacccaa aatatttcta caataaatac
     1561 tcgcatggaa ccaatggaac tctaacgttt acaatagatt taactacaaa cggttggaat
     1621 agtttttcac ttttgttcag gtataaatcg ggaaaacccg gaacagctgg atttgatggg
     1681 gttgctctta atatatggcc gggtaaaaac ggaattggag caggtcttgt aatttcgtgt
     1741 atgaacaaca ataccttggt acgacgcgat ggtgaaacat caccacttag ttatagtgct
     1801 cctgctacac cattaaccat aacggttcgt ttgttagatg ataaaatgag tgtatgggtg
     1861 aattctgacc ctgaaacaaa tacaccatta gtaacatatt cgggattaaa aattcaggct
     1921 ggttatgtag gattttataa tggaaaatct gattcaggat tgagtggtgg tggagaaaat
     1981 tcgaataatt atcgtattaa aaaatgggat tcacgttttg actctgcatt tgatttagaa
     2041 atttctgatg tattgcctac cgtaacatct tttgtaagtg ctacaaactc gggaactcat
     2101 agtgccggaa aagttacatg gc
//
LOCUS       DAOTOW010000403         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40607_length_2121_cov_4.128751, whole genome shotgun sequence.
ACCESSION   DAOTOW010000403 DAOTOW010000000
VERSION     DAOTOW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40607_length_2121_cov_4.128751"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="P5243_07505"
     CDS             complement(<1..634)
                     /locus_tag="P5243_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HRS19334.1"
                     /translation="MLIVFYQRIRVFVPIIGASLLVFILANCARESRPTGGMKDTTAP
                     QVVLEKPLNNSVLVAPKKVVIAFNESVTLDKIQEQCIISPSLSEFPKISAKKNKVTIS
                     LHKTTLQPNTTYSFTFSKAIKDVNEGNTLESYSYAFSTSSVIDSCKIAGKVLYAQSLN
                     IPKNAYVLLYSNLSESAVKTQKPNYITQVTRDGSFQFNNIAAGTYAIYALE"
     gene            759..2027
                     /gene="purD"
                     /locus_tag="P5243_07510"
     CDS             759..2027
                     /gene="purD"
                     /locus_tag="P5243_07510"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRS19335.1"
                     /translation="MNVLLLGSGGREHAFAWKLSQSKLLTQLYIAPGNAGTSEIGINL
                     PFAANDFAAIKQAIKQYSISLVLVGPEDPLVKGVHDFIASDKDIQHVPVIGPKQYAAT
                     LEGSKEFAKEFMIRHNIPTAAYFTATADNIDESFSFLSKQKPPFVLKADGLAAGKGVL
                     IIDDLETAKHELELMLRGKFGAASKKVVIEECLHGIEMSIFVLTDGKNYVILPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVPFCSPELLSNIEKTIIKPTVDGLYADGIEYCGFIFFG
                     LMVSGNHAKVIEYNVRMGDPETEVVIPRIQSDLLELCLAAANKTLNNQTIQLDPRTAT
                     TVMLVSGGYPGDYENNKPITICSNNDESIVFHAGTKKIDNTVVTNGGRVIAVTSFGTN
                     KNEALKKSYNTIKNITFEGMYFRKDIGFDL"
BASE COUNT          760 a          309 c          356 g          696 t
ORIGIN      
        1 cttctaaggc atatatagcg taagtacctg cagcaatatt attgaattga aatgaaccat
       61 ctcttgtaac ttgtgttata taatttggtt tttgtgtttt tacagcagat tcagacaaat
      121 ttgaatacaa taaaacatac gcgtttttag ggatattcaa tgattgggca tatagtactt
      181 tacctgcaat tttacaagaa tctataacag atgatgtaga aaaagcataa gaatatgact
      241 ctaaagtatt accttcgttc acatctttaa tagcttttga aaatgtaaaa ctatatgttg
      301 tattgggttg caaagttgtt ttatgcaatg aaattgttac tttatttttt ttagcactaa
      361 tttttggaaa ttctgataac gatggtgaaa ttatacattg ttcttgaatc ttatcaagag
      421 taacagactc attaaatgca atcacaactt tttttggtgc aactagtaca gaattattaa
      481 gaggtttttc caaaacaact tgaggtgctg ttgtatcttt catgcctccg gttggccttg
      541 attcacgagc acaattagca agaataaaaa ccaataaaga agctccaatt ataggaacaa
      601 aaactcttat tctttgataa aaaactatta acacgcgttt atctaaatat aattactata
      661 caaaatgcaa acatacaaaa gaattttaac aatatacgag ttaaaaaaca aacaaaaatc
      721 tgtaccttcg cactttgatt attataaaaa taaaaaacat gaatgtttta ttattaggtt
      781 cgggaggtcg ggaacatgct tttgcatgga aattatcgca aagtaaatta ttaactcaat
      841 tatatatagc tccgggaaat gcaggcactt cagaaatagg tattaattta ccttttgctg
      901 ccaatgattt tgcagcaatt aaacaagcaa taaaacaata ttccatttcg cttgttttag
      961 ttggccccga agatccttta gttaaaggtg tacatgattt tatagcctca gataaagaca
     1021 tacaacatgt gcctgtaatt gggccaaaac aatatgctgc tacactggaa ggcagtaaag
     1081 aatttgctaa agaatttatg atacgtcata atattcctac tgcagcatat tttactgcta
     1141 ctgcagataa tatagacgaa agtttttcat ttctttcaaa acaaaaacca ccatttgttc
     1201 taaaagctga tggcttagct gcaggtaaag gagtacttat tatagatgat ttagagacag
     1261 caaaacacga attagaatta atgttacgag gcaaatttgg tgctgcaagc aagaaagttg
     1321 taatagaaga atgtttacac ggtattgaaa tgtcaatatt tgtattgacc gatggaaaaa
     1381 attatgtaat acttcccgaa gctaaagatt ataaacgtat tggcgaaggt gataccggat
     1441 taaacacagg aggaatggga gcagtatcgc cggtaccatt ttgttcacct gaacttcttt
     1501 caaatattga aaaaactatt attaaaccta ctgtagatgg tttatatgcc gacggaattg
     1561 aatattgtgg atttatattt tttggactta tggtttcggg taatcatgca aaagttatag
     1621 agtataatgt acgtatgggt gaccctgaaa cagaggttgt aataccacgt attcaatcag
     1681 atttattaga attatgttta gctgcagcaa acaaaactct taataatcaa acaattcaat
     1741 tagatccacg caccgcaacc actgtaatgt tagtttcggg cggatatccg ggcgattatg
     1801 aaaacaataa acctatcact atttgttcaa ataatgatga aagtattgtt tttcatgcag
     1861 gaactaaaaa aatcgacaac acagttgtta ctaatggtgg aagggttata gcggtaactt
     1921 catttggaac aaataaaaat gaagccctca aaaaatcgta taatacaatt aaaaatatta
     1981 cttttgaagg aatgtatttt agaaaagata ttggttttga tttataaaca gcatgttatt
     2041 ttcaatttta caacgaaata attttataag ttttgtctta ttacctatag taataatagg
     2101 actttggttt ccatatctat t
//
LOCUS       DAOTOW010000404         2117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40715_length_2117_cov_3.188652, whole genome shotgun sequence.
ACCESSION   DAOTOW010000404 DAOTOW010000000
VERSION     DAOTOW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40715_length_2117_cov_3.188652"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="P5243_07515"
     CDS             complement(<1..964)
                     /locus_tag="P5243_07515"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRS19336.1"
                     /translation="MKKTLLNSITMTSVLIALSIVAFGQRPTIGGYTVYYGDLHNHSN
                     VSDGTGTPATAYSYAKNTAKLDFFSLADHSGSISSTEWTDIKNQANAINQDGVFTAFY
                     GFEWSSGGQYGHVAVINTDDYCTTTSPTNTFAGLVTWLVSRPNGVAFFNHPGREDAAG
                     LEFSHFTTTPSNQFVGMELWNKGDYFVEYYYNDGYYTNDNNKGYFDEAITRGWKIGAA
                     GAGDNHSGTWGTAYPARLAILANNLTRADLLAAMQARRFFSTLDQNLSLSFKINGQEM
                     GSTMIAGNYSLQIQAADGNNEIFTEVVLFDKNHNIVSTWTPNVTN"
     gene            complement(1259..>2117)
                     /locus_tag="P5243_07520"
     CDS             complement(1259..>2117)
                     /locus_tag="P5243_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19337.1"
                     /translation="EQYRMVWLLAALAIVVGGLLLIKKRASVNAHGWGAMQYPQVILG
                     MLAIFTYVGVEVTIGSNLGELLKQPLFGGYESSQIASFISMYWGSLMIGRWAGAVHAF
                     NLQNRAKTIALITAPILAFIVVIAANTISGKDMHILYYYIICVAIQIAAMMLCKDKPA
                     TTLFVFAVLGITAMIIGLLSHGTIAIYAFLSGGLFCSIMWPAIFSLSITGLGKYTSQG
                     SAFLIMMILGGGIVPPIQGKLADILGIHQSYIIAVVCFAYLAFFALAVRKILFKQHID
                     IDSLESASH"
BASE COUNT          733 a          376 c          329 g          679 t
ORIGIN      
        1 cattcgttac atttggtgtc catgtagata caatattatg gtttttatca aataaaacta
       61 cttctgtaaa aatctcatta ttaccatcag cagcttgaat ttgtaaacta taattgccgg
      121 caatcatagt tgaacccatt tcttgaccat ttattttaaa tgataatgat agattttggt
      181 ctaaagttga aaaaaatcgg cgagcttgca tagctgctaa taagtcggca cgagttaaat
      241 tgtttgctaa aatcgctaaa cgagcaggat acgctgttcc ccaggttccg ctatgattat
      301 cacctgctcc tgcagcacca attttccacc ctcttgttat tgcttcatca aagtaccctt
      361 tattattgtc gttggtatag taaccatcat tataataata ttcaacaaaa tagtcacctt
      421 tgttccataa ttccatacct acaaattgat ttgaaggagt agtagtaaaa tgactaaatt
      481 ctaaaccggc agcatcttca cgaccgggat gattaaaaaa agcaactcca tttggtcgcg
      541 ataccaacca agtaactaat ccggcaaatg tgtttgtagg tgaagttgta gtacaataat
      601 catcagtatt aattacagca acgtgtccat attgacctcc cgatgaccac tcaaaaccat
      661 aaaatgctgt aaatacacca tcttgattta ttgcattagc ttgatttttt atgtctgtcc
      721 attcggttga tgaaatagaa cctgaatggt ctgctaagct aaaaaaatct aatttagctg
      781 tatttttagc ataactatat gcagttgcag gtgttcctgt accatctgac acatttgaat
      841 gattgtgtaa atcgccataa tacacagtgt aaccgccaat agttggtcgt tgaccaaatg
      901 caacaataga cagtgctatt aaaacactcg tcattgtaat agaatttagt aaagtttttt
      961 tcatattctt tttagtttaa attagtactg cacaaaaatg catgcaaaac agaaaagaat
     1021 atttttagaa aaatttatga tattattatt ataatgtatt gaaatactat acaatagtta
     1081 attgcatgta atatttatgt aacatttgta aacaaatatg attgtctatt gcacaattga
     1141 atatgtacaa tatttagatt tatatgtaaa atatagattt gtaagatgtt attaaatcgt
     1201 aaaaaggtaa taaactatat agaaaaagaa tttctacaat gttattaatt tgaaaaaatt
     1261 aatgacttgc cgattctaat gaatctatat caatgtgttg tttaaataaa atttttctga
     1321 ctgcaagagc aaaaaatgct aaatacgcaa aacacactac ggcaattatg tatgattgat
     1381 ggatccccaa tatatcggct aatttacctt gtataggagg cactatacct ccacctaata
     1441 tcatcataat taaaaatgct gaaccttgcg aagtgtattt gccaagtccg gtgatagata
     1501 aactgaaaat ggcaggccac ataatactgc aaaatagccc acccgataaa aaagcataaa
     1561 tagcaattgt gccatgactc aataaaccta taatcattgc tgttatgcct aatacggcaa
     1621 atacaaataa agtagtggca ggtttgtctt tacatagcat cattgctgct atttgaatgg
     1681 ctacgcagat tatatagtag tacaatatgt gcatatcttt acccgaaata gtattagcag
     1741 ctattaccac tataaatgca agtataggtg cagtaattaa agcaatggtt tttgctctgt
     1801 tttgaaggtt aaacgcatgt accgctccag cccatcggcc tatcattaaa ctaccccaat
     1861 acattgaaat aaatgaggct atttgcgatg attcatatcc gccaaagagt ggttgtttta
     1921 ataattcacc taaattacta cctatagtaa cttctacccc aacataagta aaaatagcaa
     1981 gcattcctaa tattacttgc ggatattgca tggcacccca tccgtgagcg tttactgatg
     2041 cacgtttttt tataagtaat aatccaccaa caactatagc caaagcggct aaaagccata
     2101 ccatacggta ttgttcg
//
LOCUS       DAOTOW010000405         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40762_length_2115_cov_3.119417, whole genome shotgun sequence.
ACCESSION   DAOTOW010000405 DAOTOW010000000
VERSION     DAOTOW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40762_length_2115_cov_3.119417"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="P5243_07525"
     CDS             complement(<1..571)
                     /locus_tag="P5243_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS19338.1"
                     /translation="MEDTLIDNLSIDCVIFGFDNEELKILCIKRNTEPDFGMMALPGG
                     FVYLQEDLDKAPARRLKDLTGLTNVYMKEVGTFGNVNRYPLRRVITIAYYALIKISDY
                     VIHAGEDAQEATWIPVKQVPQMVFDHNKILEAALTKLRERVRMEPIGFNLLPEKFTIT
                     ELQTLYEQILDTKFDKRNFRKKLAKMNLLI"
     gene            complement(657..1757)
                     /locus_tag="P5243_07530"
     CDS             complement(657..1757)
                     /locus_tag="P5243_07530"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRS19339.1"
                     /translation="MPEPLKNMFSQTFYTKFIEQLQPIYPINSDQFFSILFDSAWEQK
                     ELKERLQHTAFALNQCIKGDYTTKIDIITAYIHLYSQTANSNWMLNYMFFPQFIEMYG
                     LHDYKTSIAAIETITQLSSCEFAIRPFIEKYPEQTMQQMHTWAYHSHEHVRRLASEGC
                     RPRLPWAKQLTQFRLDPSPIIPILDALKHDNSMFVRKSVANNIHDISKDNPQIALELC
                     KRWKQNSKTYLIIQHGLRTLLKQGNTTALELCNFQTPQEISCTKFAITSSLITPLKPL
                     EFTCEFAHYNKSSCVLRIEYCIYFLRSNGNYHKKVFRLKQSEFTPNTPYVLHKKHSFK
                     PLTTRKHYSGIHYISIQINGKEFEKHAFNYNQ"
     gene            complement(1772..>2115)
                     /locus_tag="P5243_07535"
     CDS             complement(1772..>2115)
                     /locus_tag="P5243_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19340.1"
                     /translation="RKVYIPRGYLEEPPTTKQAFAAHINYTSPIYYPDFAIAALVYIK
                     RFYATAFIDYAYMNGAFVNKKLWSFGIELLSDFHVLHFSMPVNAGPRISILPMNNKLS
                     LEFKGSINFGG"
BASE COUNT          664 a          291 c          347 g          813 t
ORIGIN      
        1 caataagcaa attcattttt gctaattttt ttctgaagtt tcgtttgtca aattttgtat
       61 caagtatttg ttcgtagagt gtttgtaatt ctgtaattgt gaatttttcg ggtaataaat
      121 taaatccaat tggttccatt cttactcgtt cacgtaattt tgtaagagcc gcttctaata
      181 tcttgttatg gtcaaatacc atttgaggaa cctgttttac aggtatccaa gtagcttctt
      241 gagcatcttc gccggcatgt atcacataat cagatatttt aataagagca tagtatgcaa
      301 tagtaattac ccgtcggagt ggatatctgt ttacattacc aaaagtaccg acttctttca
      361 tatatacatt ggtaagacct gttaaatctt taagtctgcg tgcaggtgct ttatctaaat
      421 cttcttgtaa atatacaaat ccaccgggca atgccatcat gccaaaatca ggttcagtat
      481 tacgttttat gcacagtatt ttaagttctt cgttgtcaaa tccaaatata acgcaatcta
      541 tcgaaaggtt gtctatcaaa gtatcttcca tcagtatagt aaatcaattt atttttaaac
      601 aaaagtacat gtttttttat tgtatataaa aacatagata taaaaaatga tgctttttat
      661 tggttgtaat taaatgcatg tttttcaaat tcttttccgt tgatttgtat tgaaatatag
      721 tgaattccac tataatgttt tcgagttgtt aatggcttaa atgaatgttt tttgtgtaaa
      781 acatatggtg tgttaggggt aaattcagat tgttttaatc taaaaacttt tttgtggtaa
      841 ttaccattac ttcttagaaa ataaatacaa tattctattc gtagtacaca agaagattta
      901 ttataatgtg caaattcaca agtgaattct aatggtttta gaggtgttat aagtgatgaa
      961 gtaattgcaa atttggtaca gctaatttct tggggtgttt gaaaattgca taattctaaa
     1021 gccgtagtgt taccttgttt taataaggtt cttaatccgt gttggattat gaggtatgtt
     1081 ttactgtttt gtttccaccg tttacaaagt tctaaagcta tttgtggatt atcttttgaa
     1141 atatcatgta tattgtttgc tacacttttt cttacaaaca ttgaattatc atgttttaaa
     1201 gcatctaaaa taggaataat aggtgatgga tctaatctaa attgagtaag ttgctttgcc
     1261 catggtaatc ggggtctgca cccttctgat gccaatctgc gaacatgttc atgagagtga
     1321 tatgcccatg tatgcatttg ttgcattgtt tgttcaggat atttttcaat aaatggtcgt
     1381 atagcaaatt cacaactact tagttgtgta atagtttcaa tagctgctat agatgttttg
     1441 taatcatgta atccatacat ttcaataaat tgaggaaaaa acatataatt cagcatccaa
     1501 tttgaatttg cagtttgtga gtatagatgt atgtatgctg ttataatatc aatttttgta
     1561 gtataatcac cttttataca ttggttaaga gcaaatgctg tatgctgtaa tctttctttt
     1621 aattcttttt gttcccatgc agaatcaaat aaaatactga aaaattggtc tgaatttata
     1681 ggatatatag gttgtaattg ttctataaat tttgtataga acgtttgcga aaacatattt
     1741 ttaagtggtt caggcattca gaataggttt attatccgcc aaaattaata ctacctttaa
     1801 attctaatga caatttgtta ttcattggta aaatactaat tcgtggaccg gcattaaccg
     1861 gcattgaaaa atgtaataca tgaaaatctg ataaaagttc tattccaaaa ctccataatt
     1921 ttttatttac aaaagctccg ttcatatatg catagtctat aaaagctgta gcataaaatc
     1981 gtttaatata tactaatgca gcaattgcaa aatcaggata gtaaatagga gatgtataat
     2041 ttatatgggc agcaaacgct tgttttgttg taggtggttc ttccaaatat cctcgtggta
     2101 tatatacttt tcgct
//
LOCUS       DAOTOW010000406         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40821_length_2113_cov_2.810496, whole genome shotgun sequence.
ACCESSION   DAOTOW010000406 DAOTOW010000000
VERSION     DAOTOW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40821_length_2113_cov_2.810496"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..909
                     /locus_tag="P5243_07540"
     CDS             187..909
                     /locus_tag="P5243_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19341.1"
                     /translation="MKYIVTVFFCTFFVLIHAQTNTLDCDLTQSYTQQTIGTWGKYIS
                     QVTTQLQQNYSPELQFKRMMIRHMYIAHLLFNDSDSKEIDIQLNGMKKDLEELEKNPQ
                     FAEKCLAFRAAYNSYCAVNSPATAVYYLPKSFSYAKDAITKQPQSPYSWSEYGNLQYC
                     YALFLGGSFSDAIKAYKKSIELIEKQQKNLSCNWYYIHTLLFLAKSYEDNKQYTEANT
                     VYDKILQITPQFTAIHRWKHKL"
     gene            complement(911..2029)
                     /locus_tag="P5243_07545"
     CDS             complement(911..2029)
                     /locus_tag="P5243_07545"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRS19342.1"
                     /translation="MAQLDKYIAKIEELREHGNLRILRNEIPEDIVNLSSNDYLGLQQ
                     NKELYEEFLDEISISYYRFTAASSRLLSGNSPEYGTLESQLQTMYGRQAALVFNSGYH
                     ANCGILPALAGPNDLIVADKLVHASIIDGMRLGQAQYERFRHLDYEHLEKILSENKNK
                     FDTIFIVSESIFSMDGDRANIPLLIELKKKYNALLYIDEAHAVGVYGDTGLGLCEEYN
                     VIHDCDFIVGTFGKALASVGAFVICDEIFKTYLINHARTLIYTTALPPINLAWSQFIL
                     SQLHTFGSQRQKLIKLSKEFAQLLSVEWQSHIIPYIIGSNTDTIACSELLQQNGFYVL
                     PIRYPTVAKNTARLRFSLQAIHTLNELQKIPAILASRK"
BASE COUNT          732 a          374 c          306 g          701 t
ORIGIN      
        1 gccggagccc caatatatac agcatcggca ccatggtcaa tagctgcaat accacaattt
       61 acatttttgg caggcgaaag taattctact gtagtcattc acaatttttt agcaaatata
      121 ttttttttac atagcgaatg gttcattatt aaatgttaat tttaccaata tttttaattc
      181 acacaaatga aatatatagt aactgtattt ttttgtactt tttttgttct tattcacgct
      241 caaacaaaca ctttagattg tgatttaaca caatcatata cacagcaaac tattggaaca
      301 tgggggaagt atatatcaca agttactaca caattacaac agaattattc accagaactg
      361 caatttaaac gcatgatgat tcgccacatg tatattgctc atttattgtt taatgattct
      421 gactctaaag aaattgatat tcaattgaat ggtatgaaaa aagaccttga ggaacttgaa
      481 aaaaatccgc aatttgcaga aaaatgtctt gcctttagag ctgcatacaa ctcatattgt
      541 gcagtaaata gtccggcaac agccgtatat tatttaccaa aaagtttttc atatgccaaa
      601 gatgctatta ccaaacaacc acaatctcca tacagttgga gtgaatatgg taatttacaa
      661 tattgttatg cattattctt gggtggtagt ttttctgatg ctattaaagc atataaaaaa
      721 tcaatagaac taatagaaaa acaacaaaaa aatctttctt gtaattggta ttatattcac
      781 actttattgt tcttggcaaa aagttacgaa gacaataaac aatatacaga ggctaataca
      841 gtatatgata aaatattaca aataactcca cagtttaccg ccattcatag gtggaaacat
      901 aaactttaat ttattttcta cttgcaagga ttgccggtat tttttgtaat tcgttcaatg
      961 tatgaattgc ttgtaatgaa aaccgcaatc gtgcagtatt tttagctact gttggatacc
     1021 ttataggtaa tacataaaaa ccattttgct gcaacaattc cgaacatgca atagtatcgg
     1081 tattgctgcc aataatataa ggaataatat gcgattgcca ttcaacactc agtaactgag
     1141 caaattcttt actgagtttt ataagttttt gccgttgtga accaaaagta tgaagttgac
     1201 tcaaaataaa ctgactccat gccaaattaa ttggaggtaa cgcagtggta tatataagtg
     1261 tacgagcgtg atttattaaa tatgttttaa aaatttcatc gcaaataaca aacgctccta
     1321 ccgatgccaa tgctttacca aaggttccta ctataaaatc gcaatcgtgt attacattat
     1381 attcttcgca tagtccaagt ccggtatcac catacactcc aacagcatga gcttcatcaa
     1441 tatataataa agcattatat tttttcttta attctatcaa taatggaata ttagccctat
     1501 ctccatccat actaaaaata ctttcactta ctataaaaat agtatcaaat ttatttttgt
     1561 tttctgaaag gattttttct aaatgttcat agtcaagatg tcgaaacctt tcatattgtg
     1621 cttgtcccaa tcgcatgcca tcaataatac tggcatgtac caatttatcg gcaacaatca
     1681 aatcattcgg acctgcaagt gccggtaata taccacaatt agcatgatat ccgctattga
     1741 atactaaagc agcttgacga ccatacattg tttgcaattg tgattcaagg gtaccatatt
     1801 cgggagaatt acccgaaagc aagcgagatg aggccgctgt aaaccgataa taactgatag
     1861 aaatttcatc gagaaattcc tcatacaact ctttattttg ctgtagtcct aaataatcat
     1921 tagaactcaa attaacaata tcttcgggta tttcattccg taatattcgt aaatttccat
     1981 gttcacgaag ttcctcaatt tttgctatat atttatctaa ttgtgccata gaaatgtatt
     2041 cactcgcatt tatatatcaa aacactgttg tccgataaaa aattaagaga aggaagatgt
     2101 tagtcttcct ttc
//
LOCUS       DAOTOW010000407         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40856_length_2111_cov_4.123541, whole genome shotgun sequence.
ACCESSION   DAOTOW010000407 DAOTOW010000000
VERSION     DAOTOW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40856_length_2111_cov_4.123541"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="P5243_07550"
     CDS             <1..302
                     /locus_tag="P5243_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS19343.1"
                     /translation="MAMIESLKRHFPENVEYTLPEGGMFLWVTLPQGISAMELFQEAI
                     KQDVAFVPGDPFYTNTKEIVSTLRLNFSCVDEQTIEKGIARLGATIREFIEKQKQ"
     gene            407..2107
                     /gene="ilvB"
                     /locus_tag="P5243_07555"
     CDS             407..2107
                     /gene="ilvB"
                     /locus_tag="P5243_07555"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRS19344.1"
                     /translation="MNKQTTQITGSEALLRILIEEGVDTIFGYPGGAIMPIYDKLYDF
                     SDKLKHYLTRHEQGAVHAAQGYARATGKVGVCFATSGPGATNLVTGIADAMLDSTPLV
                     AITAQVISPLLGTDAFQEIDVIGITMPITKWNYQVTSADEIPDALAKAFYLSQTGRPG
                     PVLIDITKDAQLQMLEYSYTKCTYMRGFYPYPKLDENSVIQAAKLINEAKKPLILAGQ
                     GVNISRAQKELKEFAEKTGIPVATTILGLNDIGSDHPNYVGMLGMHGNYAPNIKTNEA
                     DVIIAVGMRFDDRVTGNVTKYAKQARIIHIEVDKAEIGKIIRPEVAIHSDAKIALQSL
                     IKVVNKNSHESWLQEFKQAHDIELQKIIIKDTQPTEGEIKMGEVIKMISDKTKGQAIV
                     VTDVGQHQMATCRYYDFREGSEIITSGGLGTMGFGLPASMGAKVSRPDKDTVVFIGDG
                     GFQMTIQELGTIAANNIGVKIVILNNNFLGMVRQWQERFFDARYSFVDIQSPDFLTIA
                     KGYGINGIKVTERKDLKAAIDTMFAKEEPFILEVKVEKKENILPMIEPGLGVADIKLD
                     "
BASE COUNT          769 a          357 c          400 g          585 t
ORIGIN      
        1 gtatggctat gatagaaagt ttgaaacgtc attttcctga gaatgtggaa tatacattac
       61 ccgaaggcgg aatgttttta tgggtaacat tgccccaagg aatttcggcc atggaattat
      121 ttcaagaagc tataaaacaa gacgttgcat ttgtacccgg cgatcctttt tatacaaaca
      181 cgaaagaaat tgtaagtaca ttacgtctaa atttctcatg tgttgatgaa caaactatag
      241 aaaaaggaat tgctcgattg ggagcaacta ttcgagaatt tattgaaaaa caaaaacaat
      301 aaacaatgaa aacaaaacat ataatgaaac gagtctgttg cacatacttt tttctttaag
      361 actcaatata caatgtaatt attaacgata tttatactaa acatatatga acaaacaaac
      421 gacacaaatt acaggctccg aagcgttatt acgcatatta atagaagaag gagtagatac
      481 catattcggg tatccgggtg gagccattat gccgatttac gataaattat acgatttcag
      541 cgacaaacta aaacactacc ttactcgcca cgaacaaggt gcagttcatg cagcacaagg
      601 ctatgctcgt gctacaggca aagtaggtgt gtgttttgcc acatcaggcc cgggagctac
      661 caatttggtt accggaattg ccgatgctat gcttgattct actccattag tagctattac
      721 agcacaagtt atttcgccac tgttaggaac agatgcgttt caagaaatag atgtgattgg
      781 tataacaatg cctattacta aatggaacta tcaagtaaca tctgccgacg aaattcccga
      841 tgctcttgcc aaagcgtttt atctttcaca aacaggtcgt cccggtccgg ttttaataga
      901 tattaccaaa gatgcacaat tgcaaatgct tgaatattca tatacaaaat gtacgtatat
      961 gcgaggattt tacccatatc caaaacttga tgaaaatagc gtaattcagg cagctaaatt
     1021 gattaatgag gctaaaaaac cattaatatt ggcaggacaa ggtgtaaata tttcacgtgc
     1081 acaaaaagaa cttaaagaat ttgccgaaaa aacaggtatt ccggttgcaa ccaccatttt
     1141 aggtttaaat gatattggtt ctgaccatcc aaattatgtt ggtatgcttg gcatgcacgg
     1201 taattatgcc cctaatatta aaaccaacga agcagatgtt ataattgctg taggaatgcg
     1261 ttttgacgat cgcgtaacag gaaatgtaac aaaatatgca aaacaggcac gtattattca
     1321 tattgaagta gacaaagccg aaattgggaa aataataaga cctgaagttg ctattcattc
     1381 cgatgcaaaa attgcattac aatctttaat taaagttgtt aacaaaaatt cacatgaatc
     1441 atggttacaa gaatttaaac aagcacatga tattgaatta caaaaaataa taattaaaga
     1501 tacccaaccc accgaaggtg aaataaaaat gggtgaggta ataaaaatga tatctgacaa
     1561 aaccaaaggt caagctatag tagtaaccga cgtaggacag catcaaatgg ctacctgcag
     1621 atattatgat tttagagaag gttctgaaat tattacatca ggtgggttag gtaccatggg
     1681 atttggatta cctgcatcta tgggagccaa agtatcgcgc cccgacaaag acactgtagt
     1741 gtttataggc gatggcggat ttcaaatgac tattcaagaa ttaggaacca tagcagccaa
     1801 caatattggg gtaaaaattg taatacttaa caataatttc cttggaatgg ttcgtcagtg
     1861 gcaagaacga ttttttgatg cgcgctattc atttgttgac atacaaagtc ccgatttttt
     1921 aacaatagcc aaaggatatg gaataaatgg cataaaagtt accgaacgta aagatttaaa
     1981 agcagcaatt gatactatgt ttgccaaaga agaacctttc atactcgaag taaaagttga
     2041 gaaaaaagaa aatatacttc ctatgataga acccggatta ggagttgctg atattaaatt
     2101 agattaacat a
//
LOCUS       DAOTOW010000408         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40882_length_2110_cov_6.599513, whole genome shotgun sequence.
ACCESSION   DAOTOW010000408 DAOTOW010000000
VERSION     DAOTOW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40882_length_2110_cov_6.599513"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="P5243_07560"
     CDS             <1..187
                     /locus_tag="P5243_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19345.1"
                     /translation="QFVKTCTTCVSHTCEAHTSGIKHWGLSGYSSVLPRIKVRCRGIL
                     SQLRNPQRFHTADRQTV"
     gene            294..1874
                     /locus_tag="P5243_07565"
     CDS             294..1874
                     /locus_tag="P5243_07565"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRS19346.1"
                     /translation="MNYIQGVSREQLTLFPTSLDSAIDATNEVRQIDMFVNSLDMSSM
                     GFAHAKLSSEGRPAYNPGDLLKLFIYGYLNRTRSSRELEKETKRNIEVMWLLKGLQPD
                     HNTISNFRRDNPEAIRKVFEQTVRIAQHHDLIGGRILAGDSVKLRAQNSKKNNYNADK
                     VKRHVEYIDKKLEEYTNQLAQADNDSERTTVQEQIAKHQTRKEHYERITQQLEESGQA
                     QVSTSDPDSRQMIVRNNITEVAYNIQTINDAKQNIILDYKTTQNNDTHALSDMVRRAV
                     DIVGHTDFVALYDKGYHTGIELAACHSMGVETLVAIPNRPVSSQAPNPAYNFEAFTYN
                     PQNDTYTCPAGATLRTNGTTYTSKTSSFKQYKTPECKRCKFQKECTTSKKNGRIVQRS
                     EHIENVERNKAMVESNPELYKKRQTIVEHPYGTIKRQWGFDHTLIKRSIERVSADVGL
                     IFTCYNLRRLLTILYSNGKRGFFEVKTHKIRAQKSQYKLFSFFNEYSQAKIKHATQCI
                     QISIQVWLFQKNTVCLSF"
BASE COUNT          792 a          401 c          397 g          520 t
ORIGIN      
        1 ccaatttgtt aaaacttgca ctacgtgtgt atcgcacaca tgcgaggcac acacaagcgg
       61 tataaaacat tgggggttaa gtggttattc gagcgttcta cctcgcatca aggttcggtg
      121 tcgggggata ctttcgcagc tccgaaatcc ccaacgattt cataccgccg accgtcagac
      181 tgtctaaaaa ctccccacaa aaaaaataca aaaaacatca aaataaatac cataatcatt
      241 tggtaatctg atacttaatt cgtattttta agcaataatt ataacagatt gctatgaatt
      301 acatacaagg tgtcagtagg gaacaactta ctctttttcc caccagtttg gatagtgcaa
      361 tagatgctac caacgaagtt cggcagatag atatgtttgt taactcgctc gatatgagta
      421 gtatgggatt tgcccatgcg aaactatcga gcgaaggtcg tccggcatac aatcccgggg
      481 atttactcaa attgtttatt tacggatatt taaatcgtac gcgttcctcg cgagagttag
      541 aaaaagaaac caagcgtaat attgaagtta tgtggttgct caaaggtttg cagcccgacc
      601 ataatactat ttctaacttt cgtagagata atccggaagc tatacgtaag gtatttgaac
      661 aaaccgtacg aatagcacag catcatgacc ttataggcgg aagaatactt gccggcgata
      721 gtgtgaaact gcgtgcacaa aactccaaaa agaataatta taatgccgac aaggtaaagc
      781 gtcatgtaga atatatagat aagaaactcg aagaatatac caatcaattg gcacaagccg
      841 ataacgatag tgagcgaaca actgttcaag agcaaatagc gaaacaccaa acacgcaaag
      901 aacactatga gcgtattaca cagcaattgg aagaaagcgg acaagcacaa gtgtcaacca
      961 gcgaccccga tagcagacaa atgatagtac gcaacaatat aaccgaagtg gcatataaca
     1021 tacaaacaat aaacgatgcg aaacaaaata taatacttga ctacaaaaca acacaaaaca
     1081 acgataccca tgctttgagc gatatggtac ggcgcgcagt tgacatagtt ggacatacgg
     1141 attttgtagc cctctacgac aaagggtatc ataccggcat agaactggca gcttgtcata
     1201 gcatgggagt tgaaacactg gtagcaattc ctaatcgtcc ggtgtcgagc caagcaccta
     1261 atcctgcata taattttgaa gcatttacct acaatccaca gaacgatacc tatacctgtc
     1321 ctgcgggtgc aacattacga accaatggaa caacctacac atcaaaaacc agttcattta
     1381 aacaatacaa aactccggaa tgtaaacgat gtaaatttca aaaagaatgt accacctcaa
     1441 agaaaaacgg tagaatagtg caacggtcgg aacatataga aaacgtagag cgcaataaag
     1501 ctatggtaga gtcaaatccg gaattataca agaaacgaca aaccatagta gagcacccct
     1561 acggaaccat aaagcggcaa tggggatttg atcatacgct cataaaacgt tctatagaaa
     1621 gagtctcggc agatgtagga cttatattta cgtgttataa cctgcggcga ttgcttacaa
     1681 ttctatattc aaatggtaag agaggttttt ttgaagtaaa aacgcataaa atacgagctc
     1741 aaaagagcca atataagcta tttagttttt ttaacgaata ttcacaagca aaaatcaaac
     1801 atgccacaca atgcatacaa ataagtatac aagtttggtt atttcaaaaa aatactgtat
     1861 gtttgagttt ttagacggac tgccgttaag tgctattgga aacaaaaacg aacattgata
     1921 aaagataaca tttttgtatt tgtgacgaaa gattattaaa tttgcaaaaa cttaaaatta
     1981 tgtataatat atcattaaaa gagataaaag caaaggttga aaagaagaaa aaaaactcta
     2041 aacttttgac ttttcttgac acagaaatac agaatgttaa aacaatatca aatgaatttg
     2101 gtgaaacatt
//
LOCUS       DAOTOW010000409         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_40892_length_2110_cov_2.836010, whole genome shotgun sequence.
ACCESSION   DAOTOW010000409 DAOTOW010000000
VERSION     DAOTOW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40892_length_2110_cov_2.836010"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="P5243_07570"
     CDS             <1..1244
                     /locus_tag="P5243_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HRS19347.1"
                     /translation="LEMSAGKEFCKYFEKQDTISYEQAQHELYSILEDAVFSRLVSDV
                     PLGTFLSGGLDSSVVSAIAAQHISGLQTFSIGFSDNPYFDETHYAQAVAKKIQSKHTT
                     IPITNKEFADAVHHVLHHFDEPFADSSAIAVYVLSKYVKQYVTVALSGDGADELLGGY
                     RKHMAHMRSFQSSLTNMVLPSIAPLLRLLPQSRNNKLFDTFRKAYRYANALKLSKQDM
                     YWLWCSLQSQHEVALLLPQYSFSEQYNKVKQYYIQGISQTSTLNDILYADQLMVLSND
                     MLPKVDKMSMAHSLEVRSPFMDYRLIAYINSLPESYKIHGTMQKRILFDACKHLLPQE
                     LYNRPKQGFEVPLHSWCTSSVLQQQIADLLSDDFIVKQEIFSLQQIQQIRQKLYSSNP
                     GDSASHVWALLVFQTWWKTYM"
     gene            complement(1615..>2110)
                     /locus_tag="P5243_07575"
     CDS             complement(1615..>2110)
                     /locus_tag="P5243_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRS19348.1"
                     /translation="IDNNIQLCTDVMIQAQNHKGTSVVEVLQNCVIFNNGAHEYYTNR
                     NEKEDRQVILEHGKAMVFGKEKNKGIVLKGMELQVVTIGKDGITEADILIHDAHNQNP
                     TIHNMLVSMKGPDFPIALGVIRDVTEPTYDDALIEQIETARNTSHIHCMDELFSSGEI
                     WEIK"
BASE COUNT          689 a          324 c          365 g          732 t
ORIGIN      
        1 agcttgaaat gagtgcagga aaagagtttt gcaaatattt tgaaaaacag gataccatat
       61 cttatgaaca agcacagcat gaattgtatt caattttgga agatgctgtg ttttctcgtt
      121 tggtttctga tgtgccttta ggtacatttt tgagcggtgg actagattca tcggtggtaa
      181 gtgctatagc ggctcaacat atttccggat tacagacttt ttctataggt ttttctgata
      241 atccttattt tgatgaaact cattatgctc aagctgttgc aaaaaaaatt cagtccaaac
      301 atactactat tccaatcact aacaaagaat ttgcagatgc ggttcatcat gtattgcatc
      361 attttgatga accgtttgct gactcgtcgg ctatagcagt gtatgtattg tctaaatatg
      421 taaagcaata tgtaactgtt gctttgtcgg gtgatggtgc cgatgaatta ctcggcggat
      481 acagaaaaca tatggctcat atgcgtagtt ttcagagttc tcttacaaat atggtattac
      541 cttctattgc tccattgtta cggttattac cccaatcgag aaacaacaaa ctttttgata
      601 cgtttcgtaa agcatataga tatgccaatg ctctaaagtt atccaagcaa gatatgtatt
      661 ggttgtggtg tagtttgcaa tcgcaacacg aagtagcttt attattacca cagtattctt
      721 ttagtgaaca atataataaa gtaaaacagt attatataca aggtatttct caaacaagta
      781 cactcaatga tattttatat gcagaccaat tgatggttct aagtaacgat atgcttccaa
      841 aggtagataa aatgagtatg gctcatagtt tagaagtgcg ttcgccattt atggattata
      901 ggcttattgc atatataaat tctttaccag aatcatataa aattcatggt actatgcaaa
      961 aaagaatatt atttgatgct tgtaaacatt tattgccaca agaattgtat aataggccaa
     1021 aacaaggatt tgaggtgcca ttgcatagtt ggtgtacaag ttcggtgttg caacaacaaa
     1081 ttgctgattt attatctgat gattttattg taaaacaaga aatattttca ttgcagcaaa
     1141 ttcaacaaat acgtcaaaaa ttatattcat caaaccccgg cgacagtgct tcgcatgtat
     1201 gggcattgct tgtgtttcaa acgtggtgga aaacctatat gtagttgctt ttatgcttct
     1261 tttttagatt ttttaatgag aagtattatt ccaaaaattt tgagtggaat agctaaatat
     1321 agaaaaatgt aagctatacg ttggttgtgt attatatatt ttacatacat agaaacagct
     1381 ataaaaagta ctcccattaa caaaggtaaa aatgtgcgaa aaggaagatg agaaaacgta
     1441 tgtaatgaat ataacagaaa tataatttta caggcaatac cgctgcataa aaatatatag
     1501 aataacaaat gagaggcaaa tatatattta tttatgaaag ccaaagtaat acatataagc
     1561 cctatatata agaatataaa atgtttgttt gaatgattct gagtaatcat gtatttattt
     1621 gatttcccaa atttcgccgc tcgaaaacag ttcgtccata caatgtatgt gtgatgtatt
     1681 acgagcagtt tctatttgtt caatcaacgc atcgtcatat gttggttctg ttacatcacg
     1741 aattacacct aaggcaattg ggaaatcggg acctttcata gaaactaaca tattgtgtat
     1801 agttggattt tgattatgag catcatgaat caaaatatcg gcttctgtaa ttccgtcttt
     1861 gcctatagtt accacttgta attccattcc tttgagaact atgcctttgt ttttttcttt
     1921 accaaaaacc atagctttac catgttcaag aataacttgt ctgtcttctt tttcatttct
     1981 atttgtataa tattcgtgag cgccattatt aaatattaca cagttttgta atacttctac
     2041 tacagaagtt cctttatggt tttgagcctg aatcattacg tcggtacaca attgaatatt
     2101 attatcaatg
//
LOCUS       DAOTOW010000410         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41061_length_2103_cov_2.793457, whole genome shotgun sequence.
ACCESSION   DAOTOW010000410 DAOTOW010000000
VERSION     DAOTOW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41061_length_2103_cov_2.793457"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..773
                     /locus_tag="P5243_07580"
     CDS             216..773
                     /locus_tag="P5243_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19349.1"
                     /translation="MKELVRCKPCGYIMEKDKLGEECPACGVSSKVFEPYKDRMSEKR
                     RKLLDLDLHPIAVHFPQTIAVFIMQFVFLTIVLPHFKITEISAFVVFLSVLLPFSVIG
                     AFVSGVIDGKLRFKKLGTIALRRKIIIGSGMIVSSVVVAFIAIKYDYTLSTKIALFVS
                     SVVTLALAISLAVTGKKLMYAEMPG"
     gene            837..1994
                     /locus_tag="P5243_07585"
     CDS             837..1994
                     /locus_tag="P5243_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19350.1"
                     /translation="MKTLRATLFIVCIATLSLSLVQCSGKKALEKGNYAEAVYKSVQK
                     LRKSPSNKKAASTLRQAYPMAVSYYKEQIEISLKSNDAFRWGNSVQYMETVNNMADEI
                     SRCPAAKKIVPSPFRYESELNTAKKNAAEECYVVGKREFDKKTRESAREAYYLFVKAD
                     GYVLGYKDVGTLIPQAKEMATLKVVLEQIPVGAGRLQISADFFQNNIEAYLNNMFNSR
                     MFIQLYLPRQAEKLKLVPDHIIRIQFDDFVVGNSKLTQKTVTVVSADSVKVGEYTKPD
                     GSKVPVKNKVTAEYTTFRKEVISKGLVDVRVIEFATKKILMQDKMPGEFIWVSEWASF
                     NGDERALSQQQLNMTKQREVQPPAPQDLFVEFTKPIYDQVTSRLSSFYSRY"
BASE COUNT          754 a          288 c          380 g          681 t
ORIGIN      
        1 ttttgtaatg aaagtattgg tttgaaagac tattcttata caagatttaa atgtgaaaaa
       61 tatgtttgca aaatttttta tactggtagt atcaataagt gcagttttgt taactgctat
      121 ttttttcata tagtagtact tctttcatat ttggctaaca aaatgaaaat taccaaacaa
      181 aaacttgtta tatttgttac caaatttatg taattatgaa agaattagta cgatgcaagc
      241 cctgtggcta tattatggaa aaagataaat taggtgaaga atgtccggca tgtggagttt
      301 ccagtaaggt gtttgaacca tacaaagacc gaatgtcaga aaaacgaaga aagttacttg
      361 atttagattt acatcctata gcagtacatt ttcctcaaac tatagctgtg tttataatgc
      421 aatttgtgtt tcttacaatt gtattacctc atttcaaaat taccgaaatt agtgcttttg
      481 ttgtattctt gtctgtatta ttaccatttt ctgtaatagg agcatttgta agtggtgtaa
      541 ttgacggtaa attacgtttc aaaaaattag gtacaattgc tttacgacgt aaaataataa
      601 taggctcggg catgatagta agttcggtag ttgtagcatt tatagcaatt aagtatgatt
      661 atacattatc aactaaaatt gcactttttg tgtcgagtgt tgttacattg gctttggcta
      721 tttcgttggc tgtaaccggt aaaaaactta tgtatgccga aatgccggga taatggcatt
      781 atctttgaaa aacgctttaa aaaattattt atttaattgt aaaacatata attgctatga
      841 aaactttacg agcaacccta tttattgtgt gtattgcaac tctttcatta tcgttggtgc
      901 aatgttcagg aaaaaaagcg ttagaaaaag gtaattatgc cgaagcggtt tataaatcgg
      961 ttcagaaatt acgtaaaagt cctagtaata aaaaagcagc atcaacactc cgccaagcat
     1021 atcctatggc ggtaagttat tataaagagc aaattgaaat atcgctcaaa agtaatgatg
     1081 cttttagatg gggcaattct gtacagtata tggaaacagt aaataatatg gccgatgaaa
     1141 tttcacggtg tcctgcggca aaaaaaatag ttccttcacc attccgatat gaatctgaat
     1201 taaatacagc aaagaaaaat gctgcagaag aatgttatgt tgtagggaaa cgagaatttg
     1261 ataaaaaaac tcgtgaatca gctcgcgagg catattattt atttgtgaaa gccgatggat
     1321 atgtgctggg atacaaagat gtaggaacgt tgataccgca agctaaagaa atggctacac
     1381 ttaaagttgt tttagaacaa atacctgtag gtgccggcag attacaaata agtgctgatt
     1441 tttttcagaa taatattgaa gcatatttaa ataatatgtt taattctcgt atgtttatac
     1501 aattatattt acctcgacaa gcagaaaaac tcaaattagt ccccgatcat attatacgaa
     1561 ttcaatttga tgattttgtt gtaggaaatt caaaattaac acaaaaaaca gttactgttg
     1621 taagtgccga cagtgtaaaa gttggagaat ataccaaacc tgacggctca aaagttccgg
     1681 taaaaaataa agtaactgcc gaatatacaa cattcagaaa agaggtaatt tcaaaaggat
     1741 tggttgatgt gagagttata gaatttgcaa ctaaaaagat attaatgcaa gataaaatgc
     1801 ccggtgaatt tatatgggtt agtgaatggg cttcgtttaa tggtgatgaa cgtgctttgt
     1861 cacaacaaca attaaatatg actaaacaac gtgaagtaca accaccagca ccacaagatt
     1921 tatttgttga atttacaaaa ccaatatacg atcaggttac ttctcgatta tcgtcatttt
     1981 attcacgata ttaaaattga atatataaag cggtaatagt aaaaacagca aaaattacac
     2041 ttgctattcc gttggtagta ccaaaagcaa tatttatttt agaaatatcg tgtggtttaa
     2101 taa
//
LOCUS       DAOTOW010000411         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41077_length_2102_cov_3.878847, whole genome shotgun sequence.
ACCESSION   DAOTOW010000411 DAOTOW010000000
VERSION     DAOTOW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41077_length_2102_cov_3.878847"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2102)
                     /locus_tag="P5243_07590"
     CDS             complement(<1..>2102)
                     /locus_tag="P5243_07590"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRS19351.1"
                     /translation="FLMKCIDKSKSYIPWFIWGIVFFVYTFTAQKTVGFWDSGQLLAS
                     AQLLQVSEAPGYPLYILIARIFSLFSSGNIQYIPFCISLVSVCAASFTVFFVYKTTEI
                     VARILISRITDISYNVSLLLIIASAGIASLSLAFSDSFWSIATQPGVYPLSVFVIILL
                     VWCMLQWQIKGTINYIACIVFMLGLSMGVNNIVWFAVPGLVVMYYFKQNSDYSVFKMI
                     GIIAGSICATALFIYLVVHGIPSIAFACDLVCVNMLNLPVNSGFVVLFILSISLGVLG
                     IYYSYIYNKKILQSVSLASVLFIIAYSINAIIIIRSAAHVPINQNCPDNAYSSKEYFS
                     RYLFSDKPLMYGNSYCAIPDSVTSGEKVYIYENNSYTHIYTEPKPLYYSQFMQFFPRM
                     WSNIPFHIEEYKRWANIEEGINTKPLYVNGKSYNSPTLKQHCVFFLKYQLQHMYIRYF
                     LWNFVGRQNDIQGNGEPHKGNWISGISFIDSMRLGDQDVLNDSLHSDSSRNTYFFIPL
                     LLGFAGIIGLWLWNKKYALVFTLLFISTGIGVVLYLNQTPLQVRERDYIYVGSYLFYA
                     ICIGLGVISLFVWLRQYFVKHALYISIAIAVVCPSLLFAYNYNDHNRRNVDIAYQYSK
                     NVLATCKPNAILFATSDNDIYPLWYLQMVEQKRTDVRVVNIHQLSKEWYIQELQNALF
                     TSSPISFGLPIQKYRIGT"
BASE COUNT          803 a          366 c          289 g          644 t
ORIGIN      
        1 tgttccaatt ctatattttt gtattggtaa cccaaatgaa attggagacg atgtaaataa
       61 tgcgttttgc aattcctgaa tataccattc ttttgataat tggtgaatat ttacaacacg
      121 cacatctgtt cgtttttgtt ctaccatttg taaataccat aaaggatata tatcattatc
      181 ggaagtggca aacaatattg catttggttt acacgttgca agtacattct ttgaatactg
      241 ataagctata tctacatttc gcctattatg atcattataa ttataggcaa ataacaaact
      301 tggacaaaca actgctattg ctatactaat atataatgca tgttttacga aatattgccg
      361 taaccaaaca aataaagaaa taacacctaa acctatacat attgcataaa acaaatacga
      421 accaacgtat atataatcgc gttcacgaac ttgcaacggt gtttgattga gatataacac
      481 aacccctatt ccggtagaaa tgaacaataa agtaaacacc aatgcatatt ttttgttcca
      541 taaccataat cctataattc ctgcaaaacc gagcaataaa ggaataaaaa aatatgtatt
      601 acgagaagag tctgaatgta aagaatcatt taatacatcc tgatcgccaa gcctcattga
      661 atctataaaa gatatgcctg aaatccaatt acctttatga ggttcaccgt ttccttgtat
      721 atcattttgt cttcccacaa aattccacaa aaaataacga atatacatat gctgtaactg
      781 atacttcaga aaaaaaacac agtgttgttt caatgttgga ctgttatacg attttccgtt
      841 aacatacaag ggttttgtat ttattccttc ttcaatattt gcccaacgtt tatactcctc
      901 tatatgaaag ggaatgttgc tccacatacg tggaaaaaac tgcataaact gtgaataata
      961 caacggtttt ggttcagtat atatatgtgt atatgaatta ttttcatata tataaacttt
     1021 ttctcccgat gttacactat ccggaattgc acaatatgaa tttccgtaca ttaatggttt
     1081 atcagaaaat aagtaacgag aaaaatactc tttactacta taggcattgt caggacaatt
     1141 ttgattaatt ggcacatgtg ctgccgagcg tataattatt atagcattta ttgaatatgc
     1201 aataataaat agtacacttg ctaaacttac actttgtagt atttttttgt tatatatata
     1261 cgaataatat atgcctaata cccccagtga tatacttagt ataaacaaaa caacaaaccc
     1321 ggaatttacg ggcaaattca acatatttac acaaactaaa tcacatgcaa atgcaataga
     1381 aggaattcca tgaactacaa gatatatgaa caatgcagtt gcacatatac tacctgcaat
     1441 tattccaatc attttaaata ctgaataatc agaattttgt ttaaaataat acattacaac
     1501 taatccggga acagcaaacc aaacaatatt attgaccccc atagataacc caagcataaa
     1561 aacaatacat gcaatataat ttattgttcc ttttatttgc cattgtaaca tgcaccatac
     1621 taaaagtata attacaaata ccgaaagtgg atatacccca ggttgtgtag ctattgacca
     1681 aaatgaatca gaaaatgcta atgatagcga agcgataccg gcagaagcaa tgattaatag
     1741 taaagaaaca ttatatgaaa tatctgtaat tcgacttatt agaatacgag ctacaatttc
     1801 agttgtttta tacacaaaaa aaactgtaaa cgatgctgcg cataccgaaa ccaaggaaat
     1861 acaaaaaggt atatactgaa tattaccgga tgaaaaaagt gaaaaaatac gagcaataag
     1921 aatatataat ggatatccgg gagcttcact tacctgaagc aactgcgcag aggcaagcaa
     1981 ttgcccacta tcccaaaaac caacagtttt ttgtgcagta aaagtatata caaaaaaaac
     2041 tattccccaa ataaaccaag gtatatacga ttttgattta tctatacatt tcattaaaaa
     2101 gt
//
LOCUS       DAOTOW010000412         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41102_length_2101_cov_3.198436, whole genome shotgun sequence.
ACCESSION   DAOTOW010000412 DAOTOW010000000
VERSION     DAOTOW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41102_length_2101_cov_3.198436"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="P5243_07595"
     CDS             complement(<1..808)
                     /locus_tag="P5243_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRS19352.1"
                     /translation="MFSQTSRETYNFNQNWKFALGHACDVQKDFNFNTGHFSYLAKTG
                     YGDGAASEKFDDRAWRNIQLPHDWCISLPLNARASNSHGNQAIGKYFPEHSIGWYRKH
                     FFIEKADTNKRIRIEFEGVYRNSKVFVNGFFVGGEESGYDSFSFDITEYLNFGDINVI
                     AVRVDATMEEGWFYEGAGIYRNVWLVKTNKLHIATNGTYIQSNIENNKAVVTIQTQLQ
                     NDSFTPQEYSIEYIITDAKGTIVKQIQVPNYQLVANLSHTTETVINIENPI"
     gene            1070..>2101
                     /locus_tag="P5243_07600"
     CDS             1070..>2101
                     /locus_tag="P5243_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19353.1"
                     /translation="MQRIVPLVFFVVCIQSLYAQYIHQDSTDVNGNTIYIGKKPIRSF
                     TIDGSYNGLIYYRQFDSLFDGNQAQEKMILSNSDGGGEQRVLRLNFSGRPTINTFFKS
                     DMFLAPSMRGPGYNTATSDRLGVEIGVNLEAQIHTKQANAYLKFGGTQFTKLSKLTLW
                     STENKGESMFERSPWGGFQQAGQNYESYYSQGTISRDFTWGNKFLQGFTADITKLPYN
                     LESKLIYAKTPNNGGQTSYVSKNYNMTYGGNIRKKMGMSYIGFNSLNTLAYTSDVNGK
                     VYGYNVITTDGAYSFWQNDMVFSGEIGECRFVESSDLSEHWAYAMDFSLLSKSNILSA
                     PVTFHYYYIK"
BASE COUNT          719 a          305 c          339 g          738 t
ORIGIN      
        1 taatgggatt ttctatgtta ataacagttt ctgttgtatg agatagattt gcaactaatt
       61 ggtagtttgg tacttgtatt tgtttcacaa tagtaccttt ggcgtcagta attatatatt
      121 caattgaata ttcttgtggt gtaaacgagt cgttttgtaa ctgtgtttga atagtaacaa
      181 cagccttatt attttcaata tttgattgta tgtatgtgcc atttgttgca atatgaagtt
      241 tatttgtttt taccagccat acatttctat atatacctgc tccttcgtaa aaccatcctt
      301 cttccatagt tgcatctaca cgaacagcaa ttacatttat atcaccaaaa ttgaggtatt
      361 ctgtaatgtc aaagctaaaa ctatcatatc cgctttcttc tccacctaca aaaaaaccat
      421 ttacaaaaac ttttgaatta cgatatactc cttcaaactc tattcgaata cgtttatttg
      481 tatcggcttt ttctataaaa aagtgttttc tataccaacc tatactatgt tcgggaaaat
      541 attttccgat agcttgattt ccatggctat tacttgctcg tgcattgagt ggcaatgaaa
      601 tgcaccagtc atgaggtaat tgaatatttc tccatgctct gtcatcgaat ttttctgaag
      661 cagctccatc accatatccg gtttttgcta aatatgaaaa atgacctgta ttgaaattaa
      721 aatctttttg tacatcacat gcatgtccca aagcaaattt ccaattttga ttaaaattat
      781 atgtttcacg gcttgtttgt gaaaacatta cagtatgtat tcctaatatt ataagaacac
      841 ataatgataa tagttgtttg cgaaaaaaca tcaattaaaa ctattttaaa taagattaca
      901 aaaacgtatt aactccaaat tggatacaat aatacaaaaa gtttgtgtta aaaatacaat
      961 aacatgatgt attttgtaaa aatagtttta tatatttgta taatctttag ttttgagaat
     1021 acttggagat tttttattac taaataaata ctgaaatttt acatttaata tgcaaagaat
     1081 tgttcctctt gtcttttttg ttgtatgtat acaaagttta tatgctcaat acattcatca
     1141 agattctacc gatgtaaacg gcaatactat atatataggg aaaaaaccaa ttcgtagttt
     1201 tactatagat ggttcataca atggtttgat ttactatcga caatttgata gtttgtttga
     1261 tggcaatcaa gctcaagaaa aaatgatatt gagtaacagt gacggaggag gagaacagcg
     1321 tgttttacga ttgaattttt ccggacgacc aactataaat acatttttca aatcagatat
     1381 gtttttggct ccaagtatgc gaggccccgg atacaataca gctacatcgg atagattggg
     1441 ggttgaaata ggagtaaatt tagaagcaca aatacataca aaacaagcaa atgcatattt
     1501 aaaatttggt ggaacacaat ttacaaaatt gtcaaaactt acattgtgga gtaccgaaaa
     1561 taaaggtgaa agtatgtttg aacgcagccc atggggtgga tttcaacaag caggtcaaaa
     1621 ttatgaatca tattattctc aagggactat ttcgcgagac tttacatggg gtaataaatt
     1681 tttacaaggg tttactgccg acattactaa attaccttat aatttagaat caaaacttat
     1741 atatgccaaa acacctaata atggcggtca aacttcatat gtgagtaaaa attataatat
     1801 gacatatgga ggtaatattc gtaaaaaaat gggtatgagt tatatagggt ttaattcatt
     1861 aaatactctt gcttatacaa gtgatgtaaa tggtaaagta tacggataca atgtaataac
     1921 aacagatgga gcatattcat tttggcaaaa tgatatggta ttttcaggtg aaattggaga
     1981 atgtagattt gttgaatcat cggatttgtc agaacattgg gcctatgcta tggattttag
     2041 tctgttgtca aagagtaaca tactttcggc acctgttaca tttcattatt attatataaa
     2101 a
//
LOCUS       DAOTOW010000413         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41122_length_2100_cov_4.185330, whole genome shotgun sequence.
ACCESSION   DAOTOW010000413 DAOTOW010000000
VERSION     DAOTOW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41122_length_2100_cov_4.185330"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1551
                     /locus_tag="P5243_07605"
     CDS             211..1551
                     /locus_tag="P5243_07605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRS19354.1"
                     /translation="MNITIVGTGYVGLVSGACFAETGITTTCVDIDTSKIERLQKGII
                     PIYEPGLDNLVVQNFQKKRLLFSTQLSECLHNCSAVFIAVGTPPDEDGSADLQYVLAV
                     AREIGQHMTDYLVIITKSTVPIGTAEKVRNACQEELNKRGVSIPFDVASNPEFLKEGA
                     AIDDFMKPERIVIGVDSDRAEQVLRKIYKPFLLNGHPIIFMNIPSAEMTKYAANSMLA
                     TRISFMNDIANLCEIVGADIHMVRKGIGSDSRIGSKFLYAGIGYGGSCFPKDVKALIK
                     TGKKHGYDLKVLEAVEQVNEKQKEVLFDKIITYFEGNVIGKKIALWGLSFKPNTDDMR
                     EAPSLVIIQKLLEQGAKIYAYDPVAMHETKRIIGDIITYCNQPYECLNNAEALIVATE
                     WQEFRMPNFEIMAKIMKRKVIFDGRNIYETQDMLDNGFDYFGIGKKPIIQTSCV"
     gene            1582..>2100
                     /locus_tag="P5243_07610"
     CDS             1582..>2100
                     /locus_tag="P5243_07610"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS19355.1"
                     /translation="MKRILVTGGAGFIGSHLCERLLNEGNDVICLDNFFTGSKQNILH
                     LLPNPHFELVRHDVTTPYYAEVDQIYNLACPASPVHYQYNAIKTIKTSVMGAIHMLGL
                     AKRVKATILQASTSEVYGDPQVHPQREDYWGHVNPIGIRSCYDEGKRCAETLFMDYHN
                     QNNVKIKIIRIFN"
BASE COUNT          759 a          333 c          380 g          628 t
ORIGIN      
        1 gttaatgctc atggtgctat tcaaccatat gaaactctta tttttgaggt ggaattactt
       61 gctacaaaaa aaggtgaagc tcctaaaatg aacatgaaag ccatgcaaca aatgatgggt
      121 caataaaaca atgtaacaca tactaagaca catacaatct ccttgtatgt gtctttttat
      181 atatatcaat atacaaatta taattacacc atgaatatta caattgtagg tacaggatat
      241 gttggtttag tttcgggagc ttgttttgca gaaacaggaa taactacaac atgtgtagat
      301 attgatactt caaaaataga acgattacaa aaaggtataa taccaatata tgaaccgggt
      361 ttagataatt tagtagttca aaatttccaa aaaaaacgat tactttttag tacacaatta
      421 tcagaatgtt tgcataattg cagtgccgta tttattgctg taggaacacc acccgatgaa
      481 gatggcagtg ccgacctaca atatgtactt gctgtagctc gcgaaattgg tcaacacatg
      541 actgattatc ttgtaataat taccaaaagc acagtaccca taggaactgc cgaaaaagtt
      601 cgtaatgctt gtcaagaaga acttaacaaa cgcggagttt ctattccgtt tgatgtagct
      661 tcaaatccgg aattcctaaa agaaggtgcg gctattgatg attttatgaa acccgaacgt
      721 attgttatag gggtagattc tgatagagca gaacaagttt tgagaaaaat ttataaacct
      781 ttcttactca atggtcatcc tattattttt atgaatattc cttcggcaga aatgaccaaa
      841 tatgccgcaa attcaatgct tgctacgcgc ataagtttta tgaacgacat cgcaaattta
      901 tgtgaaattg ttggagctga tatacatatg gttcgcaaag gtataggtag tgattctcgc
      961 ataggaagta aatttcttta cgccggtatc ggttatggag gttcatgctt tcctaaagat
     1021 gtaaaagctc ttataaaaac cggaaaaaaa catggatatg accttaaagt attagaagca
     1081 gtagaacagg ttaatgaaaa acaaaaagag gtattgtttg ataaaattat aacatatttt
     1141 gagggtaatg taataggtaa aaaaattgca ttatgggggc tatcatttaa accaaatacc
     1201 gatgatatgc gcgaagctcc atcgttagtt ataattcaaa aattactaga acaaggtgca
     1261 aaaatttatg catatgaccc tgtggctatg catgaaacaa aacgaataat tggagatata
     1321 attacctatt gtaaccaacc ttacgaatgc ctaaataatg cagaagctct gatagtggca
     1381 actgaatggc aggagtttag aatgccaaat ttcgaaatta tggcaaaaat aatgaaacgt
     1441 aaagtaattt ttgacggaag aaacatttac gaaacgcaag atatgcttga caatgggttt
     1501 gattattttg gtattgggaa aaaaccaatt attcaaactt catgtgtata ataataaaaa
     1561 ctgaaatacc atatataaat aatgaaacga attcttgtaa ccggaggagc cggatttatt
     1621 ggttctcatt tatgtgaacg acttctaaat gaaggtaatg atgtaatatg tttagataat
     1681 ttttttaccg gaagtaaaca aaatatattg catttattac caaaccctca ttttgaactt
     1741 gttcgtcatg atgtaactac cccctattat gccgaagtag accaaatcta taatcttgca
     1801 tgccccgctt caccggtgca ttaccaatat aacgctatta aaaccatcaa aacatcagta
     1861 atgggagcta ttcacatgtt ggggcttgca aaacgtgtaa aagcaactat attacaagct
     1921 tcaaccagcg aagtgtatgg cgaccctcaa gttcacccac aacgcgaaga ttattgggga
     1981 catgtaaatc ctattggcat tcgctcatgt tatgatgaag gaaaacgttg tgctgaaaca
     2041 ttatttatgg attatcataa tcaaaacaat gtaaagatta aaatcattcg tatatttaat
//
LOCUS       DAOTOW010000414         2099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41155_length_2099_cov_3.011252, whole genome shotgun sequence.
ACCESSION   DAOTOW010000414 DAOTOW010000000
VERSION     DAOTOW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41155_length_2099_cov_3.011252"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1505
                     /locus_tag="P5243_07615"
     CDS             72..1505
                     /locus_tag="P5243_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRS19356.1"
                     /translation="MKKTVTINVNGTMFHFDEDAYAVLQDYLQTLEKHFNSENGSVEI
                     MQDIEARIAELLLMKIKRPEQVVTIHDIQEIITIMGTPEDFGIHTNTNEASNSQANAP
                     HVKRLYRDEDGRILGGVCAGMAYYFAVDVVLIRIIFIISFFFAGPLIYLAAWLIIPKA
                     RTTSQKVEMRGEKIDIESIKARFKQGFEEVKQNSTQFVRQTWRENKVSAFFIELGGHM
                     LRAVFTISILIVIAVCIALFLGLSLSWVDFSGIVSVLPQGVDACLQYLFADTYQSNLV
                     VLGCMIFILLPIFLLIWGMFSLLLGIKKNSKIIGYISFIIWFAGFCMIGFGGYGVYVD
                     FTKREVISQTYSKETSAQKFVIHKEGFANNISCNYIGSNIYHMKHWACIECVEQTICA
                     TEVEMQVDYKKQDSITMQIYYAARGKSQEIAHARAQALECKPIITDSTIVLPKFVALE
                     PGNQWRNQRVQVFITLPEGTAYSVVEK"
     gene            complement(1536..>2099)
                     /locus_tag="P5243_07620"
     CDS             complement(1536..>2099)
                     /locus_tag="P5243_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRS19357.1"
                     /translation="KKLSISIKCRLGQEESSEFETLIPIFNQFPLFEIIIHARTGIMQ
                     YKGNVLLTEYSHLASQLKHRVCYNGDIIHPSDILKIQDISPYTHACMIGRGIIKNPFL
                     LHEIRNITISKKEKIHILQAFHLSIIEYCSQKYSGDFSILKRLEEMWTLHHEAFEDGK
                     KILKQIKKCKNIAQYKNIILHSIDSIL"
BASE COUNT          677 a          266 c          377 g          779 t
ORIGIN      
        1 ttcgtggaat gaattgcttg cgtctgtttc tattgtatta caaaataaat aactcataaa
       61 ctataattgc tatgaaaaaa actgttacaa tcaatgttaa tggaactatg tttcattttg
      121 atgaagatgc ttatgctgta ttacaagatt atttacaaac acttgaaaaa cactttaatt
      181 ctgaaaacgg ctctgtggaa ataatgcaag atattgaagc tcgtatagca gaattattgc
      241 ttatgaaaat taaacgtcct gaacaggttg taactataca tgatatacaa gaaataatta
      301 caattatggg aactcccgaa gattttggga tacataccaa taccaatgaa gcatcaaatt
      361 cgcaagcaaa tgcaccacac gtaaaacgat tatatagaga tgaagacggg cgtattttag
      421 gtggtgtatg tgccggtatg gcatattatt ttgctgttga tgtagtgtta atacgaatta
      481 tatttattat ttctttcttt tttgcaggtc cgcttattta tttggctgct tggttaatta
      541 tccccaaagc acgtactaca agtcaaaaag ttgaaatgag aggtgaaaaa attgatattg
      601 aatctattaa ggcgcgtttt aaacaaggtt ttgaagaggt gaaacaaaat tcaacgcaat
      661 ttgttaggca aacatggcgt gaaaataagg tgtctgcttt ttttatagaa ttaggaggac
      721 atatgttacg agctgttttt acaatttcaa ttttaattgt aattgctgtt tgtattgcac
      781 tatttttggg gctttcttta agttgggttg atttttcggg aatcgttagt gtgcttccac
      841 aaggtgttga tgcttgtttg caataccttt ttgcagatac atatcaaagt aatttggttg
      901 ttttggggtg tatgattttt attttattac cgatattcct tctaatttgg ggaatgtttt
      961 cattgttatt gggaattaag aaaaattcaa aaattattgg atatatatcc tttattattt
     1021 ggtttgcagg tttttgtatg ataggttttg gtgggtatgg tgtatatgta gattttacta
     1081 aacgtgaagt aatttcacaa acatattcaa aagaaacttc ggcacaaaaa tttgtaatac
     1141 acaaagaagg atttgcaaat aatatttctt gtaattatat aggttctaat atctatcata
     1201 tgaaacattg ggcttgcatt gaatgtgttg aacaaacaat ttgtgcaact gaagttgaaa
     1261 tgcaagttga ttacaaaaaa caagactcta ttacgatgca aatatattat gcagctcgcg
     1321 gaaaatcaca ggaaattgct catgcacgtg ctcaagcatt agaatgtaaa ccaattatta
     1381 cagattcaac aattgtgtta cctaaatttg ttgcattaga accggggaat caatggagaa
     1441 atcaaagggt tcaagttttt attactttgc ctgaaggaac tgcatattct gttgttgaaa
     1501 aatgatagca tttataatca tttattaatg tatggttaaa gtattgaatc tatagaatgt
     1561 aatattatat ttttatattg tgcaatattt ttgcattttt tgatttgttt gagtatcttt
     1621 ttcccatctt caaatgcttc atgatgaaga gtccacattt cttcaaggcg tttaagtatt
     1681 gaaaaatcac ctgaatattt ctgtgaacaa tattctatta tgctcaaatg aaatgcttgt
     1741 aaaatatgta ttttctcttt ttttgaaatt gtaatatttc gtatttcgtg taataaaaaa
     1801 ggatttttta ttataccgcg tccaatcata catgcatgcg tataaggtga aatgtcttgt
     1861 atttttagaa tgtcgcttgg gtgtataata tcaccattat aacataccct atgtttaagt
     1921 tgtgatgcta aatgagagta ttcggtaagt aatacattgc ctttgtattg cattattcct
     1981 gtacgagcat gaattattat ttcaaacaaa ggaaattgat taaatattgg aataagtgtt
     2041 tcaaattctg aactttcttc ttgacccaat cggcatttga ttgatattga gagtttttt
//
LOCUS       DAOTOW010000415         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41170_length_2098_cov_4.113069, whole genome shotgun sequence.
ACCESSION   DAOTOW010000415 DAOTOW010000000
VERSION     DAOTOW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41170_length_2098_cov_4.113069"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1379)
                     /locus_tag="P5243_07625"
                     /pseudo
     CDS             complement(136..1379)
                     /locus_tag="P5243_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
     gene            complement(1450..>2098)
                     /locus_tag="P5243_07630"
     CDS             complement(1450..>2098)
                     /locus_tag="P5243_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRS19358.1"
                     /translation="KVLVPLLKKGGNTLIAMVSEKKSYLAEKSDYILYAHIEREACPH
                     NLAPTNSTTAQLVMGDALAMCLLEQRKFSPQDFAKYHPGGSLGKRMYLRVEDIYTQKK
                     APRVLPNQTITEVIFEISSKRMGAAAVVDTNNVLLGIITDGDLRRMLEKNTNFSTLSA
                     QDIMTKNPKTISPQDMAIAAFERMEQNSITQLAVVEKDIYKGMLHLHDLLKEGIV"
BASE COUNT          642 a          383 c          336 g          737 t
ORIGIN      
        1 aggtgatgtg ttcgacgtta aattaatagg aattgacaaa ggtaaattta aattatctaa
       61 aaaagcgttg attccgaaac ctcactaata gcaaataaga aataaaaaag gcggaaattg
      121 taattcccgc cttttttatt tatcagaatc tttccataga tacaatacat cgcctcgtcg
      181 aacgagttgg ggaaccttca tagaaaaaac atctcctttt ttacaacgga aacaggttgt
      241 aaattgtagc gaatttcatc aacggtacat tcaactacac ccgttgtttt ccctataatc
      301 aatacgtcat tgccaatgtg taactcacct gattgcatag taaattctgc aacacctata
      361 tttgaaaaat aattggtgca tttaccgagt aagtgtttaa ttttggtcgc actactacca
      421 tattgagtgc tccattctcc taatttttgc cctaaataat atccgtccca aaaacctcta
      481 ttaaatactt gagataattt atgtttccat tgctctacca attcaggtgt atatgtattg
      541 ttgcaaacgg catcaactgc ttcactgtaa cattgagtaa ctgtttttac atattcggcg
      601 cttcgagctc ttccttcaat tttaaaaaca cgcactcccg catgtattaa ttcatcaaca
      661 aaacctatag tgcataagtc tttgggggac ataatgtatt cattatctat ttcaagttga
      721 ttgccacttt ctttttctgt tactgtataa gctttgcggc atgtttgtaa gcatgctcct
      781 ttatttgcag aataattttg ttcatgtaaa cttaaatagc atttgcccga tatagacatg
      841 cataaagcac catgaacaaa catttcaatt tgcactaaat tacctgaagg tccggtaatg
      901 ttttgttcgc agatggcggt gtaaattttt ttaacttgta taagatttaa ttcacgtgca
      961 agtactacta catcggcata ttgtgaaaaa aatctaacag cctcggtatt cgatacattt
     1021 aattgtgttg aaatatgaac ttcaactcct atttggcgag catattgtat agcagcaata
     1081 tctgaagcaa taattgctgt tacaccataa tgttttgctg catcaactac tttgtgcagt
     1141 gtatccatat cagagtcata cagtacagta tttactgtta aatatgtttt aacaccatgg
     1201 ctattgcata ttgaaactat ttcagccaaa tcgtgagtag taaaattatt tgaagaacgt
     1261 gctcgcatat tgagcatttc tattccaaaa tatacagaat ttgctcctgc ttgtatagca
     1321 gccataagag attcgtatga acctaccggc gacattattt ctatgttgtt tctttgcata
     1381 gtattgtata ataataagat gtatgacaga gaaattcata tttaattaca actctctgtc
     1441 caattttaat tacacaattc cttcttttaa taaatcgtgt aagtgtaaca tgcctttata
     1501 tatgtctttt tcaactacgg caagctgagt aatgctattt tgttccatac gttcaaaagc
     1561 tgcaattgcc atatcttgag gtgaaatagt ctttgggttt tttgtcataa tatcttgagc
     1621 cgaaagcgtg ctaaaattag tatttttttc gagcattctt cgtaaatcgc catcggtaat
     1681 tattcctaaa agcacattgt ttgtatcaac tacagctgct gctcccattc gttttgatga
     1741 aatttcgaat ataacttctg ttatagtttg attaggtaat actcgtggtg cttttttctg
     1801 agtataaata tcttcaacac gtaagtacat tcgtttgcct aatgaacctc cgggatgata
     1861 tttagcaaaa tcttgaggtg aaaatttacg ttgttccaat aaacacatag ccaaagcatc
     1921 gcccattaca agctgggctg tagtactgtt tgtaggagcc aaattatgcg ggcatgcttc
     1981 acgttcaata tgtgcatata gaatatagtc tgacttttcg gctaaatatg attttttttc
     2041 agaaaccatt gcaattaacg tattgccacc tttttttaat aatggaacca atactttt
//
LOCUS       DAOTOW010000416         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41453_length_2088_cov_2.976390, whole genome shotgun sequence.
ACCESSION   DAOTOW010000416 DAOTOW010000000
VERSION     DAOTOW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41453_length_2088_cov_2.976390"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /gene="argS"
                     /locus_tag="P5243_07635"
     CDS             complement(<1..894)
                     /gene="argS"
                     /locus_tag="P5243_07635"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRS19359.1"
                     /translation="MNIESIIVDKVIEAVKALYNVDIETSAIQIQETRKEFEGDKTVA
                     VFPFVRFSKKSPEQTAAEIGDYLQNSVSHIQSYSVVKGFLNILLSHSYWIEILQQAIE
                     QGDMYGTKPITANSPVVMVEYSSPNTNKPLHLGHIRNNLLGYAIAQILAANGNKVIKA
                     NLINDRGIHICKSMIAWKLFGNGETPQSSGLKGDHLVGKYYVLFDATYKKQIQELQAQ
                     GKTEDEAKQEAPIMLQARELLVKWEANDAETIELWKTMNNWVYDGFNTTYTRMGVSFD
                     KMYYESQTYLLGKQVVIDGLAA"
     gene            complement(991..1881)
                     /locus_tag="P5243_07640"
     CDS             complement(991..1881)
                     /locus_tag="P5243_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS19360.1"
                     /translation="MISILIPVYNYSVVRLVSDLHAQAIALPCPFEIIVADDCSDAEY
                     IEQNAAIEKLLNVRYIQVSENMGRARIRNFLAHEAQFQYLIFMDCDAEVRSDTFLQTY
                     YTYCTNTVVVCGGTAYDFQKPEKNKMLRWRYGLQREQISAHQRNIRPNASFSTFNFLI
                     SKSVIIQIPFNESIREYGHEDTLFGFELYKHGYKILHIDNQLIHKGLDENTVFVEKTK
                     RAIEGLFTLLHNPAIDQAFFSDITLARFYSKVIQYRLRSPIKLLFILMHLFFKTWLIR
                     GTAPLRIYDFYKLGYMCTLS"
     gene            complement(1894..>2088)
                     /locus_tag="P5243_07645"
     CDS             complement(1894..>2088)
                     /locus_tag="P5243_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19361.1"
                     /translation="PLYSIYSHIVYAMQGHDVDTVIINGAIVYLQKQFQLVDPIETMI
                     DAQMIANAIMQRKSEREQQQ"
BASE COUNT          650 a          358 c          316 g          764 t
ORIGIN      
        1 ggcagctaaa ccgtctatta caacttgttt accgagtaaa tatgtttgcg attcataata
       61 catcttatca aatgaaactc ccatgcgtgt atatgtagta ttaaatccat catataccca
      121 attattcata gttttccaca attctatggt ttctgcatcg ttggcttccc attttaccag
      181 taattcacga gcttgaagca ttattggcgc ttcttgttta gcttcgtctt ctgtttttcc
      241 ctgagcttgt aattcttgaa tttgtttttt ataggtagca tcaaacaata catagtattt
      301 tcctactaaa tgatcgcctt tgagtcccga gctttgaggt gtttctccat tgccaaacaa
      361 tttccaggca atcattgatt tacaaatatg aatacctctg tcgtttatca aatttgcttt
      421 tattacctta ttgccatttg ctgcaagtat ttgagctatg gcatatccta ataaattatt
      481 gcgaatatgt cctaaatgta atggcttatt ggtattgggc gaagagtatt ctaccattac
      541 aaccggtgaa ttggctgtaa taggttttgt tccatacatg tcgccttgtt caatagcttg
      601 ttgtaatatt tctatccaat atgaatgtga taacaatata ttcaaaaaac cttttacaac
      661 tgaatatgat tgtatgtgcg atacgctatt ttgcaaataa tcaccaattt ctgctgctgt
      721 ttgctccggc gatttttttg aaaatctcac aaacggaaat acggcaactg ttttgtctcc
      781 ttcaaattct ttacgtgttt cttgtatttg tattgcagat gtttcaatat caacattata
      841 taatgctttt acagcctcta ttactttatc tacaattata ctttctatat tcatattctg
      901 atatttataa tgaaagtgca aaaaaacaaa taaagattga ttttggcaat ttattgttgt
      961 taaaaatcat gaatatttta gaagtatatg ttatgataaa gtacacatat atcccaattt
     1021 ataaaaatca tatattcgca aaggtgcagt gccacgaata agccatgttt taaaaaatag
     1081 atgcattaga ataaacaata gtttaattgg agaacgtaac cgatattgta taactttact
     1141 ataaaaacga gccaacgtaa tatcactaaa aaatgcttga tctattgccg gattgtgtaa
     1201 taatgtaaat aatccctcta ttgcccgttt tgttttttca acaaaaacag tattctcatc
     1261 taatcctttg tgaattaatt gattatctat atgcagtatt ttgtatccgt gtttgtataa
     1321 ttcaaatcca aataatgtgt cttcatgtcc atattctcgt atagattcat taaaaggaat
     1381 ttgaataatt actgattttg aaatcaaaaa attaaaggta gaaaaacttg cattgggacg
     1441 aatgtttcgt tggtgtgccg atatttgttc gcgttgtaat ccatatcgcc aacgaagcat
     1501 tttatttttt tcgggttttt gaaaatcgta tgcagtacca ccgcacacta caacagtatt
     1561 ggtgcaatag gtataatatg tttgtaaaaa tgtatcggaa cgcacctctg catcacaatc
     1621 cataaaaata agatattgaa attgagcctc gtgagccaag aaattacgaa ttcgtgcacg
     1681 ccccatattt tctgatactt gaatatatcg tacattcaat aatttttcaa ttgctgcatt
     1741 ttgttcaata tattcagcat cggaacaatc gtcggctaca ataatttcaa acggacaagg
     1801 taatgctata gcttgtgcat gtaaatcact tacaagcctt accacagaat aattatacac
     1861 aggtatgagt atagatatca caacttctta ttattactgt tgttgttcgc gttctgattt
     1921 tcgttgcatt atagcatttg ctatcatttg tgcatctatc atggtttcta tagggtctac
     1981 caattgaaat tgtttttgta aatatactat agcaccatta attataaccg tatctacatc
     2041 gtgcccttgc atagcatata ctatatgcga atatatagaa tacaacgg
//
LOCUS       DAOTOW010000417         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41516_length_2086_cov_2.566224, whole genome shotgun sequence.
ACCESSION   DAOTOW010000417 DAOTOW010000000
VERSION     DAOTOW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41516_length_2086_cov_2.566224"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="P5243_07650"
     CDS             complement(<1..206)
                     /locus_tag="P5243_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19362.1"
                     /translation="MRVQGDIVTDDNGDIVIADGDILVADCTPLHTRDILRTHRGEYK
                     QHPTIGAGIENFTDDESSEDMLRT"
     gene            complement(206..748)
                     /locus_tag="P5243_07655"
     CDS             complement(206..748)
                     /locus_tag="P5243_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19363.1"
                     /translation="MNNQTLRELKEAFIKFGKMGAKELVKQILFVAEVVDIQTDTCSI
                     QYNTLKLTNVRLCAIKDGNKSKLIVRPKVGSDVLVFDMSGGDLTDLLVLQVTDVEDIQ
                     TDKGSNGGLIQIEKLTDKINEVVDSLNDLIEAFNGHTHATATVIGQVTVSGTATKIAI
                     PSIKASKLKSSDYENPKIKH"
     gene            complement(750..1697)
                     /locus_tag="P5243_07660"
     CDS             complement(750..1697)
                     /locus_tag="P5243_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19364.1"
                     /translation="MIYDIRIGNYKLAMVEQVTITKSVEKLSDTAIITLPGTAYNKAL
                     EIEDKIKRGDSVTIQLGYNKTLVTEFEGFVDKIKNDGGSLTIEIEDNLFAYKESIPDK
                     EFKNESVRNIIAYLHTYIKGYSLACDYDFSYDSFVISNATAYDVLKKIQEEAKPNVYV
                     KNKTLHIHPQYIETFGRVIYDFAINIDAEGTELTYKRAEDRKILVVAEGKDSNGKVVR
                     AEYGTTGGDKITVSIPGVSRKETLTQLAKEAHTQKVYEGFEGSIVGWLVPYCDAGYEA
                     TIRDHEYEYKNGTYYVTEVVVEFSESGGKRTVKLGKKIK"
     gene            complement(1699..>2086)
                     /locus_tag="P5243_07665"
     CDS             complement(1699..>2086)
                     /locus_tag="P5243_07665"
                     /inference="COORDINATES: protein motif:HMM:NF039634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6046 domain-containing protein"
                     /protein_id="HRS19365.1"
                     /translation="PYRLPIDPIISLNGKNIITRRAVSKSKYAGRIKERWSQDDWIIT
                     IQGVLIGADREELALMVENIRTICEQSVTGVDIICNYINNTYGIYRMAIEDYDFPFTR
                     GEENQVFVIRGYSDSVYDLLIEQDNV"
BASE COUNT          535 a          453 c          311 g          787 t
ORIGIN      
        1 atggttcgta gcatatcttc cgaactttcg tcatcggtaa agttttcaat acctgcacct
       61 atagttgggt gctgcttata ttcgccacgg tgcgtacgca gtatatcgcg tgtgtgcaat
      121 ggtgtgcaat ctgccaccag tatatcacca tcggcaatta ctatatcgcc attgtcgtcg
      181 gttactatgt cgccctgtac tcgcattagt gttttatttt agggttttcg taatcacttg
      241 attttagttt gctcgctttt atcgatggaa ttgctatttt tgtagcagta ccactaacag
      301 taacttgacc aattacagtt gccgttgcat gcgtatgacc gttaaatgct tctatcaaat
      361 cattaagaga atcaaccact tcatttattt tgtcggtgag tttttctatt tgtataagtc
      421 cgccgttgga tcctttgtct gtttgtatat cttccacatc tgttacttgc agcactagta
      481 agtctgtcaa gtcgccacca ctcatatcaa ataccaaaac atcgcttcct accttggggc
      541 gtactataag cttcgatttg tttccatctt ttatagcaca caaacgcacg ttggtaagct
      601 tcaaagtatt gtactgtata ctacatgtgt ctgtttgtat atctactact tcagctacaa
      661 acagtatttg ctttactagt tcttttgcac ccatttttcc gaattttatg aatgcttctt
      721 tcaattcacg taaggtttga ttattcatat cacttaattt ttttaccaag ttttacagtt
      781 cgtttaccac cactttccga aaactctaca actacctcag ttacgtagta agttccattt
      841 ttatattcat actcatggtc gcgtatagta gcctcatatc ctgcatcaca gtatggcaca
      901 agccatccga ctatacttcc ttcaaatccc tcgtatactt tttgtgtatg tgcctctttt
      961 gcaagctgcg taagtgtttc ttttctgcta actcccggta tgcttactgt aattttatca
     1021 ccacctgttg tgccatattc tgcacgcact actttaccat tggaatcttt accttctgct
     1081 actaccaata tttttctgtc ttctgcacgt ttatatgtca gttctgtacc ttcagcatct
     1141 atgttaattg caaaatcgta aataacacga ccaaatgttt ctatgtattg cggatgtata
     1201 tgtagtgttt tgttttttac atatacattt ggcttagctt cttcttgtat tttttttaga
     1261 acatcatacg ctgtagcatt gcttattaca aaactgtcgt acgaaaaatc ataatcacag
     1321 gctaggctat atcctttaat atatgtatgc aaataagcaa tgatattacg aacactttca
     1381 ttcttaaact ctttgtcggg aatagattct ttgtatgcaa acaaattatc ttctatttca
     1441 attgtgagcg aaccaccatc gttttttatt ttgtcaacaa acccctcaaa ttcggtaacc
     1501 aatgttttgt tataacccaa ttgtatagtt acagaatcgc cgcgttttat tttatcttct
     1561 atttcaagag ctttattata tgcggttccg ggcaatgtta taatagcagt atccgaaagc
     1621 ttttcaacac ttttggttat tgttacctgc tctaccatgg caagtttata gtttcctatt
     1681 cgtatgtcat atatcatatt atacattgtc ttgctctatt agcaaatcat acacactatc
     1741 gctgtagcca cgtattacaa acacttgatt ttcttcgcct cgtgtaaacg gaaaatcata
     1801 gtcctctata gccattctat aaataccata ggtattattt atgtaattac atataatatc
     1861 aacacccgta acactctgct cacatatagt tcgtatattt tcaaccatga gtgccaactc
     1921 ctctctatct gcacctatca aaacaccctg tattgttata atccaatcgt cttggctcca
     1981 tcgttctttt attcttcctg catatttcga tttgcttacc gcacgcctgg taataatatt
     2041 tttaccgttt aaacttatta taggatctat aggaagtctg tatggc
//
LOCUS       DAOTOW010000418         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41570_length_2084_cov_3.319862, whole genome shotgun sequence.
ACCESSION   DAOTOW010000418 DAOTOW010000000
VERSION     DAOTOW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41570_length_2084_cov_3.319862"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2084)
                     /locus_tag="P5243_07670"
     CDS             complement(<1..>2084)
                     /locus_tag="P5243_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19366.1"
                     /translation="YCYKQTGQFIEAEEVYKELMKQYGKKEPIFIFEYANALRSSSKY
                     AEAITFFEQYKKIRPNDPLAEQCIQSCIQAEQWLVETPEYFVRIIPPLNTETSDISPV
                     LKGDTLIFSSSRAGSVKKTIDVKDGGKSTMLDLFYVNLQDSTLKTSNKGYLKNLNTYM
                     HEGPATFSADGNTVYFTRTVRGKKDKKGAPVTLQIYVSRRLASGEWSESKSALAINSL
                     KYSVCHPSLSADGKKLYFASDMPGGKGGTDIYVCELNKQGQWGSPKNLGEYVNTIGNE
                     LTPFIFNDSTLYFSSDFHPGMGKKDIFVAYYKDKEWRDVENLKPPINTLANDFGFVRF
                     PNENKGFLTSDRFNGIGREDIYSFINTEPITIQFATSHFAIKDNSFFNTASYQLDFGE
                     GKDKIDIVSNKKEFRFTIQDTSLVILQERREGLPYNRINLQFKKNEDAYFELNVRPMQ
                     VPVKWNGYVKQSFIKDSVTKDSTFKITAYKPVEGITVKLMSKNNLIEEVESAKNGFYE
                     FQTEFQSRKQYTIIAQKKHTDSFTYKGVVKNHESEIIPNAQVFLYQKNQVVDKLRATK
                     EGEFQFRVKKGESYRIVGRAENYELNYIDIKPSDSSDVQKIRVLTLRPKGIQPNPITS
                     PTTKPAQEIVTVPETKPQEVKKIEKFYTVLIYESIAKRPTDVLKKEYNISDSISVYYD
                     ANLYKYTIGKFDS"
BASE COUNT          599 a          328 c          342 g          815 t
ORIGIN      
        1 gaatcaaatt ttccgattgt atatttatat aaatttgcat cgtaatatac agatatagag
       61 tcagatatgt tgtattcttt ttttaacaca tctgttggtc gttttgctat actttcatat
      121 attaaaacag tataaaattt ttcaattttt ttgacttctt gtggttttgt ttccggaaca
      181 gtaacaattt cttgtgcagg tttagttgtt ggagaagtaa taggatttgg ttgtatacct
      241 ttggggcgta atgtaagtac tcttattttt tgaacatcac tactgtcaga tggttttata
      301 tctatataat ttaattcata attttctgct cttccgacta ttctatacga ttcccctttt
      361 tttactctga attgaaattc accttctttt gtagctctta atttatctac aacttgattt
      421 ttttgatata aaaatacttg tgcgttagga ataatttcag attcatgatt ttttacaaca
      481 cctttatagg taaatgaatc ggtatgtttt ttttgggcaa ttattgtata ttgttttcgt
      541 gattgaaatt cagtttgaaa ttcataaaaa ccatttttgg cactttcaac ttcttcaatt
      601 aaattgtttt tactcataag ttttaccgta atgccttcta caggtttgta tgcagtaatt
      661 ttaaacgtac tgtcttttgt tacagaatct tttataaaag attgctttac gtatccattc
      721 catttaacag gcacttgcat aggacgtaca tttaattcaa aatatgcgtc ttcatttttt
      781 ttgaattgta gattaattct gttatatggt aatccttcgc gtcgttcttg taatataacc
      841 aatgatgtgt cttgaatcgt aaatctgaat tcctttttat ttgaaacaat atctattttg
      901 tctttgcctt caccaaagtc taattggtat gatgcggtat tgaaaaaact attgtctttg
      961 atggcaaaat gtgacgtagc aaattgaata gtgataggtt ctgtatttat aaaactataa
     1021 atatcttcac gtccaatacc attgaatcgg tctgaagtta aaaaaccttt attttcgtta
     1081 ggaaatctaa caaacccaaa atcatttgca agagtattta ttggaggttt taggttttct
     1141 acatcgcgcc attctttgtc tttgtagtat gcaacaaaaa tatctttttt acccataccc
     1201 gggtgaaaat cagaggaaaa gtaaagagtt gaatcgttaa aaataaaagg agttaattca
     1261 ttgccaatag tattaacata ttcacccaaa tttttgggac taccccattg tccttgtttg
     1321 ttaagttcgc atacgtatat gtcagtaccg cctttgccac ctggcatatc cgaagcaaaa
     1381 tatagttttt taccgtctgc cgataatgaa gggtggcata ccgaatattt taaactattg
     1441 atagctaatg cactttttga ttcagaccat tcacccgatg ctaaacgtcg cgaaacataa
     1501 atttgtaatg ttacaggagc tccttttttg tcttttttac ctctcacggt acgagtaaaa
     1561 tatacggtat tgccatcggc agaaaaagtg gcagggcctt cgtgcatata ggtgttgaga
     1621 tttttaagat atcctttgtt gctggttttc aatgttgagt cttgtagatt tacataaaat
     1681 aaatcaagca ttgtcgattt accaccatct ttgacgtcaa tggttttttt tacgcttcct
     1741 gctctcgatg atgaaaaaat gagagtatca cctttgagca caggagaaat atctgaagtt
     1801 tcggtattga gtggtggtat gattcgcaca aaatattccg gtgtttctac aagccattgt
     1861 tctgcttgaa tacacgattg aatacattgt tctgctaatg ggtcattagg acggattttt
     1921 ttatattgtt caaaaaatgt aattgcttcg gcatatttag aactacttcg taaagcattg
     1981 gcatattcaa atataaagat gggttctttt tttccatatt gtttcattaa ttctttataa
     2041 acttcttctg cctctataaa ttggccggtt tgtttgtagc aata
//
LOCUS       DAOTOW010000419         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41697_length_2079_cov_3.504941, whole genome shotgun sequence.
ACCESSION   DAOTOW010000419 DAOTOW010000000
VERSION     DAOTOW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41697_length_2079_cov_3.504941"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="P5243_07675"
     CDS             complement(<1..771)
                     /locus_tag="P5243_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS19367.1"
                     /translation="MGIVRKQSIIGSIWVYAGAALGFITSAILLPRYFTQEQIGLLSL
                     LVTYGVLFAQFASAGFLHTINRLFPYFRDDSKRHNGFLFFALLVVSIASIILIGIFYI
                     VYPLIISNSSEQNNLFSHYAYLIIPLFIAIVYFNILDFYNKALFNATRGIFLKEIVLR
                     ICILLCIALYIFEYITFHAFVYSYIVSYIFILIYIIIAIFFDKQLHLQPDFSLLTKPM
                     RKEIRNVSLYGLLISAAGFIILNIDRIMIEKLVIENPLA"
     gene            complement(775..1647)
                     /locus_tag="P5243_07680"
     CDS             complement(775..1647)
                     /locus_tag="P5243_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19368.1"
                     /translation="MIHIALRIQHFLTLLAQTTVSCIRIIFLSKYPIRIPLLKTTTNC
                     IVLGNGPSLNKAIQEYGNYIKQHDSIAVNMFANTEYFEQIRPRFYVVLAPELWQEDAP
                     PHMKQEQDTLFRNIAKAQWDLYIFAPTFAQKTLFWKHYFANNHHIQIVWFNATPIEGF
                     EFFVHYTYTKLWGMPRPHNILTPALMMAIHQKYENIYILGADHSWLSELYVAETNDVY
                     LTQKHFYDEQTAQAKHMYTVQYEKRKLHEILHKFMCAFASYFVIKTYAEKQNVHIWNA
                     TQGSFIDAFDRYKL"
     gene            complement(1644..>2079)
                     /locus_tag="P5243_07685"
     CDS             complement(1644..>2079)
                     /locus_tag="P5243_07685"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRS19369.1"
                     /translation="IAALEKFDPKTQAFVDVRFKHLLSDTVFVIDSSSVISLQSYAPN
                     HLVYTSKSATKGVAVFSEIFYDKGWNAYIDGKLVPHFRTNYVLRGLEIPQGEHTIDFK
                     FEPHMYTVSNTISLIGSLLLIIALLGILFLETKKYLVHTSNE"
BASE COUNT          739 a          304 c          330 g          706 t
ORIGIN      
        1 agccaacggg ttttctatta caagtttctc tatcattata cggtctatat tgagtatgat
       61 aaaaccagca gcacttataa gtaaaccgta caaacttaca ttacgtattt cttttcgcat
      121 aggcttggta agtaatgaga aatcaggctg taagtgcaat tgtttgtcaa aaaatatggc
      181 aataattata tatattaaga taaatatgta acttacaata taactataaa caaaggcatg
      241 aaacgtaatg tattcaaata tatataatgc tatgcatagt aatatacaaa ttcgtaaaac
      301 tatttctttt aaaaatattc ctctagttgc gttaaataag gctttgttgt aaaaatcaag
      361 aatattaaaa taaacaatgg caataaataa tggaataata agatatgcat agtgtgaaaa
      421 caaattgttt tgttcactgc tattactaat tataagtgga tatactatat aaaagattcc
      481 tattagaatg atactggcaa tacttactac aagaagggca aaaaatagaa aaccattatg
      541 tcttttagaa tcatctctaa agtatggaaa taagcgatta attgtatgta aaaaacccgc
      601 tgatgcaaat tgtgcaaata atacaccata cgttacaagt aaactaagta atcctatttg
      661 ttcttgagta aaataacgtg gcaatagtat agcacttgtt ataaatccta atgcagctcc
      721 tgcatatacc catatagaac caataataga ttgtttgcga acgattccca tatattataa
      781 tttataacgg tcaaatgcat caataaatga accttgggta gcattccata tatgcacatt
      841 ttgtttttcg gcatatgttt ttattacaaa atatgaagca aatgcacaca taaatttatg
      901 taatatttcg tgtagctttc gtttttcata ttgtacggta tacatatgtt ttgcttgtgc
      961 tgtttgttca tcgtaaaagt gtttttgggt aaggtacaca tcattagtct ctgctacata
     1021 taattccgat agccatgaat ggtcggcacc taaaatatat atattctcat atttttgatg
     1081 tattgccatc ataagagcag gagttaaaat attatgagga cgaggcattc cccataattt
     1141 tgtatatgta tagtgaacaa aaaattcaaa tccttcaata ggagttgcat taaaccacac
     1201 tatttgtata tgatgattgt tggcaaaata atgcttccaa aataatgttt tttgagcaaa
     1261 tgtgggagca aaaatgtaca aatcccattg tgctttggca atatttctaa ataacgtatc
     1321 ttgttcttgc ttcatatggg ggggggcatc ttcttgccac aattccggag caagcacaac
     1381 ataaaaccga ggacgtattt gttcaaaata ttctgtatta gcaaacatat ttacagcaat
     1441 tgaatcatgt tgtttaatat aattgccata ttcttgtatt gctttattta atgacgggcc
     1501 gttacctaaa actatacaat ttgtagttgt tttgagtagg ggaatacgta tcgggtattt
     1561 tgataaaaat ataatacgaa tacaacttac tgttgtttga gcaagaagtg ttaaaaaatg
     1621 ttgtattcgt aaagctatgt gtatcattcg tttgatgtat gaaccaaata ttttttggtt
     1681 tctaaaaata gtattcctaa taatgcaata ataagtaata atgaaccaat taacgaaatt
     1741 gtattactta cagtatacat gtgaggttca aatttaaaat ctatagtatg ttcaccttgt
     1801 ggtatttcta atcctcgcaa tacataattt gttctaaagt gaggaacaag tttgccgtca
     1861 atatatgcat tccaaccttt gtcataaaat atttccgaaa aaacggcaac acctttagtt
     1921 gcagattttg acgtgtatac taaatgattg ggagcataac tttgtaacga tattaccgaa
     1981 gaactgtcga tcacaaaaac ggtatcagat agcaaatgct taaatcgcac atctacaaaa
     2041 gcttgcgttt taggatcaaa tttttctaaa gctgcaatt
//
LOCUS       DAOTOW010000420         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41812_length_2075_cov_3.325248, whole genome shotgun sequence.
ACCESSION   DAOTOW010000420 DAOTOW010000000
VERSION     DAOTOW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41812_length_2075_cov_3.325248"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="P5243_07690"
     CDS             complement(<1..413)
                     /locus_tag="P5243_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS19370.1"
                     /translation="MSYNLLKGKKGIIFGALNEMSIAWKVAERAVEEGAEIVLTNTPM
                     AIRMGEMDALATKLNAKVIAADASSVEDLENLLKESMAHFGGQIDFILHSIGMSLNVR
                     KKRPYDDLNYEYMMKSLDISAISFHKLLQTARKLDA"
     gene            complement(429..>2075)
                     /gene="recN"
                     /locus_tag="P5243_07695"
     CDS             complement(429..>2075)
                     /gene="recN"
                     /locus_tag="P5243_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRS19371.1"
                     /translation="RLYISNYALIDFLEIQCHAGLTSITGETGAGKSIILGALSLVLG
                     KRADTSVLKNSEQKSIIEIEVIITNKYVKTLFAKHDVDFEPHTTLRREITPQGKSRAF
                     INDTPVSLQALKEIGESLIDIHSQHHNLLLRESSFQLQVIDATANNNELLNSYIQEYN
                     LLLSKQKELRLFEQNMATYSSDLDYTQFRLQELEGAQLKPNELEELEQEAQILEQGEE
                     ILHLFAQAKSIFEGDFGSLSGIKTLSQAFQKKAHISKQFSELSERLTQSYIELNDIAR
                     EIEILENTLDFDPAKLDAIHSRIDVLQSLLHKHKATTISELIEEKERLQTIVDKIEHA
                     NFDSKELQEACKKQEQIVFELSKQLHDSRKQAAKKIEPQLISMLQKLGMPSVSFSIEV
                     EYTSQYLEHGCDSIIMKFSANKHIPQQPLGEISSGGELSRVMLCLKSIISTSNSLQTI
                     IFDEIDTGVSGEIADQMGAIMQDISKHLQVIVISHLPQIAAKAQFQFKVFKEETSNTT
                     ITKIVALNNEQRVEELAKMMSGKHITEAAIQNAKHLLGIL"
BASE COUNT          586 a          326 c          334 g          829 t
ORIGIN      
        1 gcatctaatt tacgagctgt ttgtaacagt ttatgaaatg aaatagctga aatatctaac
       61 gatttcatca tatattcata gtttaggtcg tcgtatggtc gttttttgcg aacattgaga
      121 gacataccaa ttgaatgaag aataaaatca atttggccac caaaatgagc cattgattct
      181 ttcaataaat tttctaagtc ttctacactc gaagcatctg cagctattac ttttgcatta
      241 agtttagtag ctaaagcatc catctcgccc attcgtatag ccattggagt atttgttaat
      301 actatttcgg caccttcttc tacggcacgt tctgccactt tccacgctat actcatttca
      361 ttaagtgctc cgaaaataat tccttttttt ccttttagta aattgtaact cattgttttg
      421 taattttttt atagtattcc taataaatgt tttgcgtttt gtattgcagc ttcggtaatg
      481 tgttttccac tcatcatttt tgctaattct tctactcgtt gttcgttatt taaagccaca
      541 atttttgtaa ttgtggtatt agacgtttct tctttaaaaa ccttaaattg aaattgtgct
      601 tttgctgcaa tttgaggtaa atgacttata acaattactt gtaaatgctt agaaatatcc
      661 tgcataattg cccccatttg atctgcgatt tctcctgaaa ctccggtatc aatttcatca
      721 aatattatgg tttgtaatga atttgaagta cttatgattg attttaaaca aagcataact
      781 cgtgaaagtt caccacccga acttatttcg cctaatggtt gttgcggtat atgtttattt
      841 gccgaaaatt tcataataat tgaatcgcat ccatgttcca aatattgtga tgtatattct
      901 acttcaatag aaaaagaaac tgaaggcata cctaattttt gaagcattga aattaattga
      961 ggttcaattt tttttgcggc ttgcttgcgg ctatcatgga gttgtttaga taattcaaat
     1021 acaatttgtt cttgtttttt gcaggcttct tgtaattctt ttgagtcaaa atttgcatgt
     1081 tctattttgt caacaattgt ttgcaatcgt tctttttctt ctatgagttc tgatatagtt
     1141 gttgctttat gtttatgtaa taatgattgt aatacatcta tacgagaatg aatagcatct
     1201 aattttgcag ggtcaaaatc gagtgtgttt tctaatattt caatttcgcg agctatatca
     1261 tttaattcta tatagctttg agtaagacgt tcgcttaatt cagaaaattg ttttgatata
     1321 tgagctttct tttgaaaagc ttgtgaaagg gtttttattc cgcttaagct accaaaatca
     1381 ccttcaaata ttgattttgc ttgggcaaac aaatgaagta tttcttcgcc ttgttctaaa
     1441 atttgagctt cttgttcaag ctcttctaat tcgtttggtt ttagttgggc tccttctaat
     1501 tcttgtaatc taaattgtgt atagtctaaa tcggatgaat atgttgccat attttgttcg
     1561 aataaacgca gttctttttg ttttgaaagt aaaagattgt attcttggat ataagaatta
     1621 agcaattcgt tattgtttgc tgttgcatct attacttgca attgaaatga cgattcacga
     1681 agcaataaat tgtgatgttg agaatgaata tcaataagag attcgcctat ttcttttaat
     1741 gcctgtaaac ttacaggagt atcgtttatg aaagctcgtg attttccttg cggtgtaatt
     1801 tcgcgtcgta aagttgtatg gggttcaaaa tctacatcat gctttgcaaa aagggtttta
     1861 acatatttgt ttgtaattat aacttctatt tctataatac ttttttgttc agaattttta
     1921 agtacactgg tatctgctcg tttacccaat actaatgaca atgctcctaa aataattgat
     1981 ttacctgcac cggtttctcc ggttattgat gttagtccgg catgacattg tatttccaga
     2041 aaatctatta atgcataatt tgatatatat aatcg
//
LOCUS       DAOTOW010000421         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_41891_length_2072_cov_2.519584, whole genome shotgun sequence.
ACCESSION   DAOTOW010000421 DAOTOW010000000
VERSION     DAOTOW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41891_length_2072_cov_2.519584"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2072)
                     /locus_tag="P5243_07700"
     CDS             complement(<1..>2072)
                     /locus_tag="P5243_07700"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19372.1"
                     /translation="VTETDGTCTSDPACMTFSIINCPVPAPTTLQPIVTACINEAIPS
                     LQVNGTQTIRWYNKLDLATIIHTGSSYTPTISNTGTYEYYVVQYDGSCEGAGINIKLT
                     VNSTPQPVVSKSPDKCEGTSTSLWTSTLPGTVHWYTSNPSSSSAVATGSTFTLPANTA
                     AGNYTIWTVHENGCKSDPVQTQINIKPLPQAPTISAQDVCYGGTITYSASSTGIVKWF
                     NSNTDITPIATGLSYTISNATGTSKTMYATQTVDGCESAVKSSKTVTINEIPSTPIGN
                     NRSICEYDDIPALTVNSESGMQFTWYSDAALTIPVSNSYIYTPTNKVTQTLYVTKTKN
                     NCVSPVKTIQFTVNPQPAKVTFVPNTDIEICENSKVTISVTSNLIVRWYDSNLPTANP
                     IAQNRFFETPILPVGTHTYYATQTNSNTGCESEKAAKLVQVIPGPTMPQLVNNSIICQ
                     GGTPGTLQVKTSNPLEKITWLSSKGEVLQQGVTYTPPSSYSQEAGIYTFYARNSVAGC
                     PTESSKQLAVTLEVQPTPLAPKVTKSTFCYTGDPIELYAKGSNVTWYNIDKSPLSHCI
                     NKETCIPGIVSSGEFTFYVSQEFNGCISPLGEVKTVISKPPKPEIIGDKHVCSNTTEI
                     YTIKNYDKDNTITWKVTGNRPIYDVANVESAYTRSIDWLAPGVDTVIVTEQNKYGCSG
                     TYQLQVFV"
BASE COUNT          583 a          331 c          401 g          757 t
ORIGIN      
        1 aacaaaaact tgtaattgat atgttccgga acaaccatat ttgttttgtt ctgttacaat
       61 tacggtatca actcccggag caagccaatc aatagaacgt gtatatgcag attctacatt
      121 agcaacgtca taaataggtc tgtttccggt aactttccat gttattgtat tgtctttatc
      181 ataattcttg attgtataaa tttcagttgt gtttgaacac acatgtttat caccaattat
      241 ttcaggtttt ggaggtttac ttattactgt ttttacttcg ccaagaggac ttatacatcc
      301 gttaaattct tgacttacat aaaacgtaaa ctcaccgctg gaaactattc ccggaatgca
      361 cgtttcttta tttatacaat gcgataaagg tgatttatct atattatacc aagttacatt
      421 gcttcctttt gcatatagtt caattggatc gcctgtataa cagaaggtac tttttgtaac
      481 tttaggagcc agtggtgttg gctgcacttc taatgtaacg gcaagttgtt tagatgattc
      541 tgttggacaa ccggctactg aatttcgggc atagaatgtg taaattccgg cttcttgact
      601 ataacttgaa ggaggagtgt aagttactcc ttgttgtaaa acttctcctt tactactgag
      661 ccatgtaatt ttttctaaag gatttgaagt tttaacttgt aatgttcccg gagttcctcc
      721 ttgacaaatt atactgttgt ttactaactg tggcattgtt ggtccgggta ttacttgtac
      781 aagttttgct gctttttcac tttcacaacc ggtgttactg ttagtttgtg ttgcataata
      841 tgtatgagtt ccaacaggaa gtattggtgt ttcaaaaaat ctgttttgag caattggatt
      901 agctgtaggc aaattactat cataccatcg aacaattaaa ttacttgtaa cacttattgt
      961 tacttttgaa ttttcacaaa tttcaatatc tgtatttggc acaaatgtaa ccttagcagg
     1021 ttgtggattt actgtaaatt gaattgtttt tacaggactt acgcaattgt ttttggtttt
     1081 ggttacatat aatgtttgag tcactttatt tgttggtgta taaatataac tatttgaaac
     1141 aggtatagta agagcagcat cggaatacca tgtaaattgc attcccgatt cactatttac
     1201 agtaagtgca ggtatgtcat cgtattcaca aatgcttcta ttattaccaa taggagtaga
     1261 tggtatttcg ttaattgtaa cagtttttga agattttacg gcgctttcac aaccatcaac
     1321 tgtttgtgtt gcatacatag ttttacttgt acctgttgca tttgagattg tatatgaaag
     1381 tcctgttgct ataggggtaa tatcagtatt agagttaaac cattttacta tacctgtaga
     1441 tgaagcagaa tatgtaatag ttccgccata acaaacgtct tgagcagaaa tagttggtgc
     1501 ttgtggcaat ggttttatgt ttatttgagt ttgaacagga tcacttttac atccattttc
     1561 atgaacagtc catatagtat agtttcctgc tgcagtattt gcaggtaatg taaatgttga
     1621 acctgttgct acggcacttg aacttgaagg gtttgatgtg taccaatgaa cagtaccggg
     1681 tagtgttgaa gtccatagag aggtagaagt tccttcgcat ttgtcgggac ttttgcttac
     1741 aacaggttgc ggtgttgaat ttacggtaag ttttatatta atacctgcac cttcgcatga
     1801 accatcatat tgaacaacat agtattcata agttccggta tttgatattg ttggtgtata
     1861 tgaagaacct gtatgaatta ttgtcgcaag gtctaattta ttataccaac gtatggtttg
     1921 agttccgttt acttgtaaac tgggtatagc ttcgttaata catgcagtaa caattggttg
     1981 caatgtagta ggagcaggaa caggacaatt tataatagaa aatgtcatac acgcaggatc
     2041 acttgtgcaa gtgccgtcag tttcggttac ac
//
LOCUS       DAOTOW010000422         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42136_length_2062_cov_2.995516, whole genome shotgun sequence.
ACCESSION   DAOTOW010000422 DAOTOW010000000
VERSION     DAOTOW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42136_length_2062_cov_2.995516"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..944
                     /locus_tag="P5243_07705"
     CDS             213..944
                     /locus_tag="P5243_07705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRS19373.1"
                     /translation="MELDLHSIDISNILFLDIETVPQYAVYIDVPEKDRRLWDVKSKR
                     LQTANERPSVLYKKAGIFAEFGKIICISVAYVVSKKNQPLAVRVRSFYGDNERVILEQ
                     FLQLITKHFNTTKHYLCAHNGIEFDFPFIARRALIHGLVLPKTLDTRGKRPWDIRHLD
                     TLDLWRFGDYKNYTSLELLTHIFDIPTPKDDIDGSMICDVYWKENDFQRIVEYCQKDV
                     VAVLQLLRKYRNEPLFEQQSITIVS"
     gene            969..1991
                     /locus_tag="P5243_07710"
     CDS             969..1991
                     /locus_tag="P5243_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRS19374.1"
                     /translation="MITIHFPAEWHMQDMVQLTWPHKQTDFAYVYNDAVTCFCTIARE
                     ISKRQKLLIICKNKTEVQQQLGEILNTSIFFFECDSNDVWARDHGPITVYHNSMPVLY
                     DFAFNGWGKKFAYDKDTVITEKLCNSMFFTHAVRQDYSQFVLEGGSIESNGIGVLLTT
                     EACLLSKQRNPHLQKSAIETFLKDIFGAHTVLWLKHGFLQGDDTDSHIDTLARFVDEH
                     TICYVQCTDENDLHYNELRAMEHELQSFVTEIGEPYHLVPLPMVPPQYDEDGERLPAT
                     YANFLILNNAVLLPIYNCKTDDEAIRIMQTLFPTREIVPIDCRVLIKQHGSLHCVTMQ
                     YPKGVL"
BASE COUNT          718 a          310 c          385 g          649 t
ORIGIN      
        1 agtatgcgta aagaacaatc gtatttgaaa cggaatacat tgtaactaac tctgtacaaa
       61 tggataatga tatcttgaaa tagggtataa cagaaacaac attatacaaa tacaatgtaa
      121 tacactgtat aataccgtta ttattttcta tttttttata tctttgtatc aacgtataca
      181 aatctattaa tcaataacaa taatccattt ttatggaact tgacctgcat tctattgata
      241 tttctaatat tttgtttttg gatatagaaa cagtaccaca gtatgctgta tatatagatg
      301 ttcccgaaaa ggacaggagg ttgtgggatg tcaagtctaa acgattgcaa acagcaaatg
      361 aacgccctag tgtattgtac aaaaaagcag gaatttttgc tgaatttggt aaaattatat
      421 gtatttcggt tgcgtatgtt gtgagtaaaa aaaatcaacc gcttgctgtt cgtgttcgtt
      481 cgttttacgg agataatgaa cgtgtaattt tagaacaatt tctgcaatta attaccaagc
      541 atttcaatac aaccaaacat tacttgtgtg cacataacgg tattgaattc gattttccgt
      601 ttattgctcg acgtgcactt attcacggtt tagtactgcc aaaaacactc gatacgcgtg
      661 gtaaacgtcc gtgggatata cgacatcttg atactcttga tttatggcga tttggcgatt
      721 acaaaaatta tacatcgctc gagttattaa cacatatatt tgatataccc actcctaaag
      781 acgatataga tggcagtatg atatgcgatg tgtattggaa agaaaatgat tttcaacgta
      841 ttgtagaata ctgtcaaaaa gatgtagttg ccgtattgca gttgttgaga aaatatagaa
      901 atgaaccgct gtttgaacaa caaagtatta caatagtttc gtaattttgt aataaattca
      961 gtaatagaat gattacaata cattttcctg ccgaatggca tatgcaagat atggtgcaat
     1021 taacatggcc acataagcaa acagattttg cctatgtgta caatgatgct gttacgtgtt
     1081 tttgtactat agcacgagaa ataagcaaac gacaaaaatt attaataata tgtaaaaaca
     1141 aaaccgaggt acaacagcag ttaggtgaaa tactcaatac atcaatattc ttttttgaat
     1201 gtgacagtaa tgatgtgtgg gctcgcgatc atggaccaat aacagtgtat cataattcaa
     1261 tgcctgtgtt atatgatttt gcgtttaatg gttggggtaa aaaatttgcg tatgataaag
     1321 acactgtaat tacagaaaaa ttgtgtaatt caatgttttt tacccatgcc gtaaggcaag
     1381 actattctca atttgtttta gaaggaggat caatagaatc aaacggtata ggagtattgc
     1441 ttacaaccga agcatgtttg ttaagtaaac agaggaatcc gcatttgcaa aaatcggcaa
     1501 tagaaacatt tttgaaagat atatttggtg cacatactgt tttatggctc aaacacggtt
     1561 ttttacaggg cgacgacacc gatagtcata tagatacact tgcacgattt gttgacgaac
     1621 atacaatttg ctatgtgcag tgtactgatg aaaatgattt gcattacaat gagttgcgag
     1681 ctatggagca tgaattacaa agttttgtaa cggaaatagg cgaaccatat catttggtgc
     1741 cactgcctat ggtgcctccg caatatgatg aagacggaga acgattgcct gctacgtatg
     1801 ccaatttttt aatactcaat aatgccgtat tgttgccaat atacaattgt aaaactgatg
     1861 atgaagcaat tcgtatcatg caaacattgt ttccgacacg agaaatagta ccaatagatt
     1921 gcagagtatt gattaaacag catggttcat tacattgtgt taccatgcag tatcctaaag
     1981 gagtattata aattaaaaag taatatagtg gcagtaaaag tgagaaaaat aacagcagta
     2041 gtaataaata catatataga ac
//
LOCUS       DAOTOW010000423         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42174_length_2061_cov_3.127617, whole genome shotgun sequence.
ACCESSION   DAOTOW010000423 DAOTOW010000000
VERSION     DAOTOW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42174_length_2061_cov_3.127617"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1273)
                     /gene="dprA"
                     /locus_tag="P5243_07715"
     CDS             complement(164..1273)
                     /gene="dprA"
                     /locus_tag="P5243_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRS19375.1"
                     /translation="MHSQNSLYYIATTMLPGIGPVLARQLISYLGSVEQIFKESPKNL
                     AKIPKIGETLAQYIKENNVMQQAEKEFLFIQKNNIHIAIYTDADYPHRLKNCDDAPLL
                     LYYKGNNNFNHSKIISIVGTRSATEYGKRCCKELIADLQKHNPIVVSGLAMGIDICAH
                     KEALKNGLETIAVLGTQLSTVTPASHIKYAREIIAHKGTVLSECHSQTIYDNALYVKR
                     NRIIAGMSDITIIVESKTRGGALITAEYANQYNRDVAAFPGNIYNTYSQGCNMLIKQN
                     KAHLIESAQDVERILNWDIDAHTSTKTIQKQLFVDLQGEELTVFSLLQQSKELTIDQI
                     ATQSNIPMSKTSSILLQLEFKGYIQAFPGKRFSLA"
     gene            complement(1281..>2061)
                     /locus_tag="P5243_07720"
     CDS             complement(1281..>2061)
                     /locus_tag="P5243_07720"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19376.1"
                     /translation="NVIIENTNKGIITPLFPLSDIPLTKTDISEKLISIPKQEIKYLL
                     FDEEWFMDTAAFIMNKNVRSYTLVREYLRKTPFDSEEQSKSLIAKIPCIDTVSKSYKD
                     LELLSKNVAYEVSLVNESNPEYLENIQVKHVMNVIIAKALSGSVPVYSFMLRDTLQLL
                     TKEDVKMRLGELTESYVNVDETTGAEDTVTIEKKIDPSEFTALAFIEDWYINPKTMHI
                     YKDVKGIALVRQYMKQLDGNEPEMVKTIPILMYFSNHTEGK"
BASE COUNT          621 a          319 c          332 g          789 t
ORIGIN      
        1 ggagtagagt cgggagttga agaagcatta cgatttatgt gtgttggcga tagtgttcgg
       61 ttacttattc caccgcatct tgctcatggg cttataggag accaagtttg tataccaaag
      121 ttagcaattc ttaattatac acttgttgtg aaaaaacgtt atcctaagca agactaaatc
      181 gtttgccggg aaatgcttgt atataccctt taaattctaa ttgtaatagt atagatgatg
      241 ttttactcat gggaatatta ctttgtgttg caatttgatc aatagtaagt tcttttgatt
      301 gttgtaaaag agaaaaaact gttagttctt cgccttgtaa gtcaacaaat aattgttttt
      361 gaatagtttt tgttgatgta tgtgcatcaa tgtcccaatt taatatacgt tcaacatctt
      421 gcgctgattc tatgagatga gctttatttt gttttatgag catattacag ccttgtgaat
      481 atgtattata aatatttccc ggaaaagcag ctacatctct attgtattga tttgcatatt
      541 cggcagtaat aagagctcca ccgcgtgttt tactttcaac aataatagta atatcactca
      601 taccggcaat tattctattt cgttttacat acaaagcatt atcatatata gtttgtgaat
      661 gacattctga aagtacagtt cctttatgtg caataatttc gcgagcatat ttaatatgcg
      721 atgccggagt tacagtactt aattgagttc ctaatactgc aatagtttct aaaccatttt
      781 ttagggcttc tttgtgagca catatatcaa tacccatagc aagtccgctt acaacaattg
      841 ggttatgttt ttgtaaatca gcgattaatt ctttgcagca tcgtttgcca tactctgtag
      901 cacttcgagt tcctacaata cttattattt tagagtgatt aaagttgtta ttgcctttat
      961 aatataataa taaaggggca tcgtcacagt tttttaacct atgcggataa tcagcatctg
     1021 tataaattgc tatatgaata ttattttttt gaatgaacag aaactctttt tctgcttgtt
     1081 gcattacatt gttttctttg atgtattgtg ctaatgtttc tccaattttt ggaatttttg
     1141 ctaaattttt gggagattct ttaaaaatct gttcaacact acctaaatag gatatgagtt
     1201 gtcgagccaa taccggtcct atacccggca gcattgtggt agcaatataa tataaactgt
     1261 tttgcgaatg cattagtata ctatttacct tctgtatgat ttgagaaata catcaaaata
     1321 ggtatagttt ttaccatttc cggttcgtta ccatcaagtt gtttcatata ttgccgtaca
     1381 agtgcaatcc cttttacatc tttgtaaata tgcatagttt taggatttat ataccaatcc
     1441 tctataaatg ctaaagcagt aaattcgctt ggatcaattt ttttctcaat ggttacagta
     1501 tcttcggctc ctgttgtttc atcaacatta acgtatgatt cggtaagttc acctaatcgc
     1561 atttttacat cttcttttgt tagaagttgt aatgtatcac gtaacataaa actataaaca
     1621 ggtacagaac ctgaaagagc ttttgcaatt ataacattca taacatgttt tacctgaata
     1681 ttttccaaat attctggatt agattcattt actaatgaaa cttcataggc aacatttttg
     1741 ctcagtaatt ctaaatcttt atatgacttt gaaacagtat caatacatgg tattttagca
     1801 attaatgatt tactttgttc ttcgctatca aagggagttt tgcgtaaata ttcacgcaca
     1861 agggtatatg aacgaacatt tttattcata ataaatgctg cagtatccat aaaccactct
     1921 tcatcaaata ataggtattt aatttcttgt tttggtattg aaattaattt ttctgaaata
     1981 tctgtttttg taagaggtat atcagataat gggaataagg gagtaataat tcctttatta
     2041 gtattttcaa ttatgacatt t
//
LOCUS       DAOTOW010000424         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42225_length_2059_cov_3.535928, whole genome shotgun sequence.
ACCESSION   DAOTOW010000424 DAOTOW010000000
VERSION     DAOTOW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42225_length_2059_cov_3.535928"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="P5243_07725"
     CDS             complement(<1..1272)
                     /locus_tag="P5243_07725"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRS19377.1"
                     /translation="MQIQTVKLIESIQYLSVVGNLPATISGIETDSRKISQGFVFVAI
                     QGVAVDGHIFIEKAIEQGASVIVCQHVPQKIIDSVAYVVVENSAVACGYILRAYYEID
                     FSLFTVVGVTGTNGKTTTATLLYQVFEALGYKAGLISTVRNYIHTQAIESTHTTPDTV
                     QLYSLFNAMIEQGCTHCFMEVSSHAIHQHRIAGIDFTGGIFTNLTQDHLDYHKTFAEY
                     LLVKKSFFDGLPMAAFVLSNVDDKNGEIMLQNTKAHKYTYSLKGFSDFKCKILESHFD
                     GMLLTIDGTEVWTKFIGAFNAYNILSVYAAAILLGQPKQSVLQIISTLNPVDGRIQFF
                     RNSSGTTAVVDYAHTPDALENILTSLKKLCGTNNKIITVVGAGGNRDTSKRPIMGKIA
                     AQLSDTLIITSDNPRNEEPQTIAQQMYDGVPE"
     gene            complement(1283..>2059)
                     /locus_tag="P5243_07730"
     CDS             complement(1283..>2059)
                     /locus_tag="P5243_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS19378.1"
                     /translation="NNGKMMRPYLVREIRNSATTVLRNEPKVVNNAVCSEQTIQKAKL
                     LMEAVVEEGTAKSIKNKYYKIAGKTGTAQTYVNGKYDEKILRASFCGYFPAENPKYSC
                     IIVMQSALPYDPYRISGGGAAQVFRKIADRIYFNDHELRNELATDSGKVVTLPSVSNG
                     FANDYTVIFNTLGIPQASTPKSNWTNTKLQEASIDYTPLVFDDKTVPNLFGMGMRDAV
                     YIAENAGLTVSCKGYGRLVRQSIPPGTAIRRNETIVLEFN"
BASE COUNT          647 a          396 c          300 g          716 t
ORIGIN      
        1 ttctggcaca ccatcataca tttgttgagc aattgtttgt ggttcctcat tacgaggatt
       61 gtcagaagta attatgagtg tatcactcaa ttgagcagca attttcccca taattggtcg
      121 tttacttgta tctctatttc cacctgcacc aaccacagtt attatcttat tatttgttcc
      181 acataatttt tttaaactgg ttaatatatt ttctaaagca tcgggagtat gagcatagtc
      241 aacaacagca gtagttcctg aactgtttct aaaaaattga attcttccat ctaccggatt
      301 cagtgtgctt ataatttgta aaaccgattg ttttggttgg cctaacaata tagctgcagc
      361 atatacgctt aaaatattat atgcattaaa tgctccaata aatttagtcc atacttcggt
      421 gccatcaatt gttagaagca ttccatcaaa atgactctca agtattttac atttgaaatc
      481 actaaaacct ttgagtgaat aggtatactt gtgagctttt gtattctgca acattatctc
      541 accattttta tcatcaacat tgcttaatac aaatgctgcc ataggtaacc catcaaaaaa
      601 tgatttttta accagtaaat actccgcaaa tgttttatga taatctaaat gatcttgtgt
      661 aagatttgta aatatacctc ccgtaaagtc aataccggca attctatgtt gatgaatagc
      721 atgagaactt acttccataa aacaatgcgt acatccttgt tcaatcattg cattaaacaa
      781 agaatacaat tgaactgtat cgggtgtagt atgagttgat tcaattgctt gagtatgaat
      841 ataatttcta acagtcgaaa tcaaacctgc tttatatcct aatgcttcaa atacctgata
      901 caacaaggtt gctgtagtag ttttgccatt ggtacctgtt acaccaacaa cagtaaataa
      961 agaaaaatca atttcataat atgctcgtaa tatatatcca catgcaacag cagaattttc
     1021 tacaacaaca tatgctaccg aatcaataat tttttgaggc acatgttgac atacaattac
     1081 cgatgctcct tgctctattg ccttttcaat aaatatatgg ccatcaacag caactccttg
     1141 aatagcaaca aatacaaaac cttgagaaat tttgcgtgag tctgtttcaa tacctgatat
     1201 agtagcaggc aaatttccca ctaccgaaag atattgaata ctttctataa gttttactgt
     1261 ttgtatttgc atatttgttt acttaattaa attctaatac aattgtttca tttcggcgaa
     1321 ttgcagttcc cggaggaata gattgacgca ccagtcttcc atatccttta caactaactg
     1381 taagtcctgc attttcggca atatatacgg catcacgcat acccatacca aaaagatttg
     1441 gtactgtttt atcatcaaat accaatggag tataatcaat acttgcttct tgtaattttg
     1501 tatttgtcca attactttta ggagtagaag cctgcggaat acccaaggta ttaaaaataa
     1561 ctgtataatc atttgcaaaa ccattgctta ccgaaggtaa agtaacaact ttaccactat
     1621 cggtagctaa ttcatttcgc aattcatgat cattaaagta gattctatcg gcaatttttc
     1681 taaacacctg agcagctcct ccccccgaaa ttctatatgg gtcatacggc aatgcagatt
     1741 gcataactat gatacatgaa tatttaggat tttcggcagg aaaatatcca caaaaagaag
     1801 ctcgtaatat tttttcatca tacttaccat ttacataagt ttgagctgtt cctgtttttc
     1861 ctgctatttt gtaatattta ttcttaattg attttgctgt accttcttct acaactgcct
     1921 ccataagcaa tttagctttt tgtatggttt gttcgctgca tacagcatta ttcacaactt
     1981 ttggttcatt acgcagtact gttgttgcag agttacgaat ttctcttacc aaatacggac
     2041 gcatcatttt tccgttatt
//
LOCUS       DAOTOW010000425         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42285_length_2057_cov_3.162837, whole genome shotgun sequence.
ACCESSION   DAOTOW010000425 DAOTOW010000000
VERSION     DAOTOW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42285_length_2057_cov_3.162837"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="P5243_07735"
     CDS             complement(<1..252)
                     /locus_tag="P5243_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19379.1"
                     /translation="MLKILHIIPSLSKGGAERLVISICSELQNRTNVEVRLVLLSNTN
                     DYAQEMSTPTYDVVSAAFVPSFTKTSKRDVSQLQTYIDTY"
     gene            complement(265..1395)
                     /locus_tag="P5243_07740"
     CDS             complement(265..1395)
                     /locus_tag="P5243_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HRS19380.1"
                     /translation="MEFKIYKQCKRCVMDTSDPYITFDEHGYCNHCSEYLLKTKQHIY
                     QGDVSDAALQKIVEKIKRKGKGKKYDCILGISGGIDSCYAAYICKQLGIRVLAVHMDN
                     GWNSEAAVKNIKYVVSKLGIDYESYVLDWEEFKDLQLSFLKASVPEAETPTDIAIPAA
                     LHKIAAKYNVKYIISGGNYATEGILPKSWHYNAKDVTYIKAIQKQFGTKKLKKFPDFS
                     YKKEFYYKFIRGIRIVYILNYVPFSKESAMKTLEQELDWKYYGGKHYESKYTGFLQSY
                     ILPKKFNLDYRLATFSTQICAGSMTREQALEELKKLPYNPDTVEQEKIYWSKKFGITL
                     DELNKILELPPKNHTDYPNDQKLLEWIYNMYRWLQMKKKELL"
     gene            complement(1400..>2057)
                     /locus_tag="P5243_07745"
     CDS             complement(1400..>2057)
                     /locus_tag="P5243_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRS19381.1"
                     /translation="KLFNDLQADELVFLDILATKEKRNIPIEIIKEIGEEANMPFAVG
                     GGIYSNKQIQAYIKAGAEKVILSTHAAANPACIKEAAEEFGSSTIAVCIDVKKNLLGK
                     YTVYTQNGSQKTQYTPSEFAQIVQENNAGEIIIQSIDLDGTMKGYDIALIQSISKCVT
                     IPVVALGGAGSLQHMHQAFTQGYANGLAAGSMFVYHGSNKGVLINYPNKTERNLIFTQ
                     "
BASE COUNT          570 a          351 c          346 g          790 t
ORIGIN      
        1 atatgtgtca atgtatgttt gtaattgtga tacatcgcgt tttgatgttt tagtaaacga
       61 aggaacaaat gcagccgaca ctacatcata tgtaggtgtg ctcatttctt gagcgtaatc
      121 gttggtattg ctgagcaaca ccaaacgtac ttcaacattc gtgcgatttt gtaattcact
      181 acaaatgcta ataacaagcc gttcggcacc gcctttggaa agagaaggta ttatgtggag
      241 gatttttagc atggaacaat atagttacaa tagttctttt tttttcattt gtaaccaacg
      301 atacatattg taaatccatt ctaaaagttt ttggtcgttg gggtagtcgg tatgattttt
      361 tggaggtaat tccaaaattt tgtttaattc gtcaagtgta attccgaatt ttttgctcca
      421 gtatattttt tcttgttcaa cagtatcggg gttatatggt aattttttga gttcttccaa
      481 tgcttgttct cttgtcatag acccggcaca aatttgagta gagaaagttg caagtctgta
      541 atcgagattg aatttttttg gtaaaatata tgattgcaaa aagccggtgt attttgattc
      601 ataatgttta ccgccataat atttccaatc caattcttgt tcgagcgttt tcatagccga
      661 ttcttttgaa aaaggtacat agttcaaaat atagacaatt ctgatacctc gaataaattt
      721 gtaatagaat tcttttttgt aactaaagtc ggggaatttt tttagttttt tggtgccaaa
      781 ttgtttttga atagctttaa tataggtaac atctttcgca ttataatgcc aacttttggg
      841 gagaattcct tcggtagcat agtttccgcc gcttatgata tattttacat tgtattttgc
      901 tgcaattttg tgtaatgctg ccggtatagc tatatcggta ggagtttcgg cttcgggtac
      961 cgatgcttta agaaacgata attgcaaatc tttaaattct tcccaatcga gcacatagct
     1021 ttcgtaatct attcccaatt tacttacaac atattttata ttttttacag ctgcttccga
     1081 attccatccg ttgtccatgt gtacggctaa tactcttatg ccaagttgtt tgcatatata
     1141 tgctgcataa cagctgtcta tacctccgct aattcctaaa atgcagtcat atttttttcc
     1201 ttttcctttt cgtttaattt tttctactat tttttgtaat gcagcatcac ttacatctcc
     1261 ttgataaata tgttgtttgg tttttaaaag gtattcactg caatgattac aataaccatg
     1321 ttcatcaaat gtaatatacg gatcgctggt atccattaca catcgtttac attgtttata
     1381 tattttaaat tccatatagt tattgtgtaa atattagatt tcgttctgtt ttatttggat
     1441 aatttataag aacacctttg ttggaaccat gatacacaaa catactacct gctgccaagc
     1501 catttgcata gccttgagta aatgcttggt gcatatgctg taatgaaccg gcaccaccta
     1561 aagctacaac aggtatagtt acacactttg aaatagattg aatcaaagca atatcatacc
     1621 ctttcatagt gccatctaaa tctatagatt gtattattat ttctccggca ttgttttcct
     1681 gcactatttg agcaaattct gaaggcgtgt attgtgtttt ttgcgagcca ttttgagtgt
     1741 aaacagtata ttttccaagc agattctttt ttacatctat acatactgca atggtagacg
     1801 aaccaaattc ttcggcggct tcttttatac aggcaggatt tgctgctgcg tgcgtgctta
     1861 atataacttt ttcggctccg gcttttatat atgcttgaat ttgtttattg gaataaatac
     1921 ctcctcctac agcaaatggc atattagctt cttcgccaat ttctttaatt atttcaattg
     1981 gaatatttct tttttctttg gttgcaagta tatcaagaaa gactaattca tcagcctgta
     2041 aatcgttaaa taatttt
//
LOCUS       DAOTOW010000426         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42501_length_2050_cov_3.676190, whole genome shotgun sequence.
ACCESSION   DAOTOW010000426 DAOTOW010000000
VERSION     DAOTOW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42501_length_2050_cov_3.676190"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2050)
                     /locus_tag="P5243_07750"
     CDS             complement(<1..>2050)
                     /locus_tag="P5243_07750"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRS19382.1"
                     /translation="MVQENPSAVAGQELQDNPFEFSPEIEGKAVWVDSQTIEFIPKKR
                     MPAGEIYTVDFKLHKFIEVEKKLRVLTFRFQVMKQGIEYSFNGIEPYKTDSKKWQKIH
                     GIYTTADVTDNQELEKAVEAQANGKKFKISWQHSKDGLTHNFTIDSVERKDKPEMIQL
                     TCKAKAIGIDETYTERIEMPALHDFKVLHVKTTTNPQTVIDIFFSDPLNSTQDKTGLF
                     ILDPSANERVVVTPTGAKLFLGKSVTGDVKLTVLAGLQNSFGQTLQRDYVTTLKFVSM
                     KPEVSLVGDGIIIPNNEGILFHFNAVSLKAVDVTIIQIFENNIPQFLQVNQIDGDREL
                     KRVGRIVYNQEMPLISDKAIDYGTWNTFSLDLSQLIQPEPGAIYRIEIGFRKKHSLYP
                     CSEADNQEEEETYKPKKDINAEYDAPEQSYWDYYDEYDYEYYDYDWRDRDNPCKQSYY
                     ANNRRKVVRNVLASDFGIIAKAGTNNEYFVTVTDLRNTNPLSGIDIEFRNYQNQIIAK
                     TKTNSDGMCKIKLENKPFLLVAKKDKQRGYLRLDDASALSLSVFDVKGEELQKGVKGF
                     IYGERGVWRPGDSLYLTFILEDKNKSLPANHPVIFELYTPENQLSQRIVKSLNTNGFY
                     SFRINTAADAPTGNWTAKCKIGNSTFSKTLKIETVKPNRMKINLTYTTKILRNGVVEK
                     G"
BASE COUNT          529 a          377 c          361 g          783 t
ORIGIN      
        1 cctttttcta caaccccatt gcggagtatt ttggtagtat atgtcaggtt tattttcata
       61 cggttgggtt tcaccgtttc tattttgagt gttttactaa acgtagaatt acctattttg
      121 cattttgctg tccagttgcc ggtaggagca tcggcagcgg tgtttattct aaaactatag
      181 aatccatttg tattgagcga ttttacaatt ctttgcgaca attgattttc gggggtatac
      241 aattcaaaaa ttaccgggtg gtttgcgggt aacgatttgt ttttgtcttc taaaataaac
      301 gtaaggtaca atgaatcgcc cggacgccaa acgccgcgtt cgccatatat aaaacctttc
      361 actccttttt gtaattcttc gccttttaca tcaaacacac tcagcgaaag tgccgaagcg
      421 tcatctaacc gtaaatatcc gcgttgttta tcttttttgg caaccaacaa aaatggcttg
      481 ttttcgagtt ttattttaca catgccatcg ctattggttt tggttttagc aattatttga
      541 ttttgataat ttctaaattc tatatctata ccactcaatg gattggtatt gcgtaaatct
      601 gttactgtta caaaatattc attgttggta cctgcttttg ctataatacc aaaatcgctc
      661 gcaagcacat tgcgtacaac ttttcttcgg ttgtttgcat agtacgattg tttacatgga
      721 ttatctctgt ctctccagtc gtagtcataa tactcatagt cgtattcatc atagtaatcc
      781 caatagcttt gttcgggagc atcatattct gcattaatat cttttttagg tttatatgtt
      841 tcttcttctt cttgattgtc agcttcggaa catggataga gagagtgttt ttttctaaat
      901 cctatttcaa ttctgtaaat tgcgcctggt tcgggttgta taagttgtga caaatcgagc
      961 gaaaaagtgt tccatgtacc atagtcaata gctttgtcag aaataagcgg catttcttga
     1021 ttatatacaa tacgcccaac acgttttagt tcacggtcgc catctatttg gtttacttgt
     1081 aaaaactggg gaatattatt ttcaaatatt tgaataattg taacatctac agcttttaaa
     1141 cttactgcat taaagtgaaa aagaatacct tcgttattcg gaataattat gccatctcca
     1201 accaatgaaa cttcgggttt catgcttaca aattttagcg tggtaacata atcacgttgt
     1261 agtgtttgac caaaagagtt ttgcaatccg gcaagaaccg taagtttaac atcgccggtt
     1321 accgattttc ctaaaaataa tttagctccg gttggagtta ccaccacacg ctcatttgcc
     1381 gatgggtcga gtataaataa gcccgttttg tcttgcgtgc tgtttaatgg gtctgagaaa
     1441 aatatgtcta ttactgtttg cggatttgta gtagttttta catgtaatac cttaaaatcg
     1501 tgtaatgcag gcatttctat gcgttctgta tatgtttcgt ctatacctat agcttttgct
     1561 ttgcaggtaa gttgaatcat ttcgggtttg tctttacgtt caacactatc tatggtaaaa
     1621 ttatgtgtta atccatcttt gctgtgttgc cacgatattt tgaatttttt gccgtttgct
     1681 tgtgcttcta ctgctttttc taattcttga ttgtctgtaa catcggcagt agtataaatt
     1741 ccgtgaattt tttgccattt tttactatca gttttgtatg gttctatacc gttaaaactg
     1801 tattctatac cttgtttcat tacttgaaac cgaaatgtaa gcacacgtaa ttttttttct
     1861 acttctatga atttgtgtag tttgaaatca accgtgtata tttcacctgc aggcatccgt
     1921 ttttttggta taaattctat ggtttgacta tcaacccaca ctgctttacc ttcaatttcg
     1981 ggactaaatt caaaaggatt atcttgcaat tcttgtcctg ctacggctga aggattttct
     2041 tgtaccatac
//
LOCUS       DAOTOW010000427         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42556_length_2048_cov_2.454089, whole genome shotgun sequence.
ACCESSION   DAOTOW010000427 DAOTOW010000000
VERSION     DAOTOW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42556_length_2048_cov_2.454089"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="P5243_07755"
     CDS             <1..657
                     /locus_tag="P5243_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS19383.1"
                     /translation="GYTLTLQKLSASINVYNMQYVNQLTLTGKLNDVGDPIMENVPDS
                     YRRGIEWIVGARPVKEIAWNASLTLSRNKIHNYTEYSVNYTDTWDEEPKITELGTTDI
                     SYSPNVIASNSIMFYPDKSIYFGFTSKYIGAQFYDNTSDEQRKLPAYAIHNFILAYTK
                     PIYKTSLTIQLMVNNILDTQYISNAYGWNWYEQNQEKSQSFYYPQAGINYMCKMSLQF
                     "
     gene            727..1320
                     /gene="pnuC"
                     /locus_tag="P5243_07760"
     CDS             727..1320
                     /gene="pnuC"
                     /locus_tag="P5243_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRS19384.1"
                     /translation="MVEWIIHHWIEIAGTLFGLAYVLLEVKQHNAMWFVGIITSLLYI
                     IVFFQSQFYADMGLQVYYVFVSVYGWFMWKHGGGDHTELQIQNIRIVYALVLAIITVL
                     IFFIISYILVHYTDSPVPYWDSFTTALSITATYMLAHKYIEQWFIWMIVNGVSIWLYW
                     NKELYSTVVLFSVYFILSVIGYTVWFREHTQAKHLQL"
     gene            complement(1325..1912)
                     /gene="ruvC"
                     /locus_tag="P5243_07765"
     CDS             complement(1325..1912)
                     /gene="ruvC"
                     /locus_tag="P5243_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRS19385.1"
                     /translation="MVNEKIILGIDPGTTILGYGLISVSGKTMKLLTLGVINLQKFDD
                     HYIKLQKIYKRLQGLIQEYCPDEMALEAPFFGKNVQSMLKLGRAQGVAMAAGLAFDIP
                     ITEYAPRKVKMAITGTGTASKEQVAAMLSRLLNTEAKPEFLDATDALAVAVCHYYQIS
                     KPGITQGTSAKSWKEFLHKNPTRVITPKQKKDTKK"
BASE COUNT          629 a          294 c          372 g          753 t
ORIGIN      
        1 ggatatacac ttacattgca aaaattgagt gcttctataa atgtatataa tatgcaatat
       61 gtaaatcaat taactcttac tggtaagctc aatgatgtag gagatcctat tatggaaaat
      121 gttcctgata gctatcggcg tggtattgaa tggattgttg gagcacgacc tgtaaaagaa
      181 atagcttgga atgcttctct tacactttca cgaaataaaa tacacaatta tacagaatat
      241 tctgttaatt atactgatac ttgggatgaa gaacccaaaa ttacagaatt aggaacaaca
      301 gatatttcat attcaccaaa tgttatagcc tcaaatagta ttatgtttta tcctgataaa
      361 tctatatatt ttggatttac ttctaaatat attggagctc agttttatga taatacatct
      421 gacgaacaaa gaaaattgcc tgcatatgct attcataatt ttatattggc atataccaaa
      481 cctatctata aaacatcgct tactatacaa ttaatggtaa ataatatact tgatacacaa
      541 tatatttcaa atgcatatgg atggaattgg tacgaacaaa atcaagaaaa gagtcaaagc
      601 ttttattatc cacaagcagg gattaattat atgtgtaaaa tgagtttgca gttttaattt
      661 ttatttcttt tttggggggg ggtagtatca gctgtcagat actgctcccc aaattttaag
      721 taaattatgg tagaatggat tatacatcat tggattgaaa ttgccggcac tctattcggg
      781 cttgcgtatg tgttgctcga ggtaaagcag cataatgcaa tgtggtttgt cggaattatt
      841 acatcgttac tatatatcat tgtttttttt cagtcacaat tttatgctga tatgggattg
      901 caagtatatt atgtgtttgt aagtgtgtat gggtggttta tgtggaaaca tgggggggga
      961 gatcatacag aattacaaat acaaaacatt cgtatagtgt atgctttggt gttggccatt
     1021 attactgtac ttatattttt tattatatca tatatacttg tgcattatac cgattctcct
     1081 gtcccgtatt gggattcatt cactacagct ttgagtataa ctgcaacata tatgttagct
     1141 cataaatata tagagcaatg gtttatttgg atgattgtga acggggtgag tatttggtta
     1201 tattggaaca aagaattata tagtactgta gtgttgttta gtgtttattt tatactatct
     1261 gtaataggat atacagtttg gtttcgagag catacccaag ctaaacatct tcaattgtag
     1321 ggtattattt ttttgtgtct tttttttgtt ttggagtaat aactcttgta ggatttttgt
     1381 gtaaaaattc tttccatgat ttagcagatg ttccttgagt tattcccggt ttagaaattt
     1441 gataataatg acatacagca acagctaacg catcggtagc atcaagaaac tcgggttttg
     1501 cttcggtatt gagtaatcgc gaaagcatag ctgcaacttg ttctttagaa gctgttcctg
     1561 ttcctgttat agccattttt actttacgag gggcatattc cgttattggt atatcaaatg
     1621 ctaaaccagc cgccattgca acaccttggg cacgaccgag tttaagcata gattgaacgt
     1681 tctttccaaa aaatggtgct tctaaagcca tttcatcagg acaatattct tgaataagtc
     1741 cttgtaaacg tttataaatt ttttgaagtt ttatataatg gtcatcaaat ttttgtaagt
     1801 ttattactcc taaggttaat aatttcattg ttttccccga tacagatata agtccgtatc
     1861 ctaaaattgt tgtaccgggg tcaattccta aaattatttt ttcattaacc atgcaacatt
     1921 atattagaac tgagtttgta agtattgttt aaatgccgaa tatgttgcag gtatcgtatc
     1981 aaatgattca ggtttatctt ctatgccttg taaacttttg ggtaattcgg ggtttattcc
     2041 aatatttg
//
LOCUS       DAOTOW010000428         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42624_length_2046_cov_2.190357, whole genome shotgun sequence.
ACCESSION   DAOTOW010000428 DAOTOW010000000
VERSION     DAOTOW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42624_length_2046_cov_2.190357"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..589)
                     /locus_tag="P5243_07770"
     CDS             complement(257..589)
                     /locus_tag="P5243_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS19386.1"
                     /translation="MIYTLGETVFDIVFRNMNPITAKVGGSALNTAVSLAHLGASVSF
                     VSEMGTDTVGKHCKEFLKQQGVNTSYISTHSTHPTSLALAFLNQTNDAQYQFYKQYQS
                     YSIVNLYK"
     gene            1175..2002
                     /locus_tag="P5243_07775"
     CDS             1175..2002
                     /locus_tag="P5243_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19387.1"
                     /translation="MKTLLLNSGLARVSCISITLSLLVIVVQANSLHQTPPSSPATVI
                     TVDVCSNNNWFDVPVEITQSVEQCLGFDIELTFDAQVAIPTGTVLFNNSLIDARFISY
                     MVTVGNNTMNISVFLNGKGALQTFNGKGTIVTVEFQKQNHFDSPFIVSSSVLESYETY
                     TVKKNLLPIQCIENSCGSEDENENKTPVMYGVNNEFISELKVTAFPNPVIHTLQIQSN
                     QTVHVIIKDATNQVKGVYSNISPNSVSSINMSQYKSGIYFITVYNKAQSKKLCVVKN"
BASE COUNT          692 a          291 c          321 g          742 t
ORIGIN      
        1 taaatacgta ctgtgtttgt atctccaaat attgcagcac aatcttcgtc tgatgctctt
       61 acaatatgac attttgaaaa gttttcttct atataattca tctcagtaga tcgagcatga
      121 acctgtgacc ggatatttgg atcatatact atcagtgact tttgtaattg tgcagtatat
      181 agtatttgta ataatgattc gcgtgttcgt tcacttatag caaatgatga agaaaatagt
      241 acaatatctt tatgtgttat ttgtaaaggt ttacaattga atagctttgg tattgtttgt
      301 aaaattgata ttgagcatcg tttgtttgat ttaaaaaagc taatgctaat gaagtaggat
      361 gtgttgaatg tgtagaaata tatgatgtgt ttacaccttg ttgttttaaa aattctttac
      421 aatgttttcc tacagtatcg gttcccattt cagatacaaa tgataccgaa gctcctaaat
      481 gagctaaact tacagcagta tttagggcag aacctcctac ttttgcagtt ataggattca
      541 tattcctgaa tacaatatca aatacagttt cgcctaaagt atatatcata ttcttttttc
      601 tgtaaaaata taaaaaaaaa agaggctaca ctattgtaaa cctctttttg ccccaagcat
      661 tggtgttacc ggaacacctt caaatgtata tacctctgtg ttatattgaa aaacatgagt
      721 gcaattcatc atattcgtgg gtgtaatttt tttgaaccca atgttctaat tgctttgtct
      781 catctgtgtt gaggtatgaa atacattttt ttaactcctt tctaaagagt tctttgtcaa
      841 aacttaattt ctgtaaaatt acatacgcgt attctaacat gtttttttgt taataataat
      901 ttaacataaa tatacaatag ttataactcc tttgcaagta aaatattata ttgaataggt
      961 gatataaatc aacgcttgct ggtatcattt tttagtgcat ttttgaaact ttttgtaata
     1021 ttaaatttaa caattattta aaaaacttaa cataaaatta aaaacaacta aaattccgga
     1081 ttcgtacaag tcaataaatg cgatatacat tattgtttgg agtgcaaatt tcttgaagtg
     1141 cggagtcagc acggtaaaca ctgacgacta agctatgaaa acactattac ttaattcagg
     1201 attagctcgt gtaagttgta taagtataac tttgagttta ttagtcattg tagttcaggc
     1261 aaattctcta catcaaacac caccatcatc acctgcaact gttataactg tagatgtgtg
     1321 ttcaaataac aattggtttg atgtgcctgt agaaattacc caatctgtag agcaatgttt
     1381 ggggtttgat atagaactta cttttgatgc tcaggtggca attccaaccg gtaccgtttt
     1441 atttaataat tcacttattg atgcacggtt tatttcatat atggttactg ttggtaacaa
     1501 tacaatgaat attagtgtgt ttttaaatgg gaaaggagct ctgcaaacat ttaatggtaa
     1561 aggaacaata gttacagtag aatttcagaa acaaaatcat tttgattctc cctttatagt
     1621 atcatcttcg gttttggaaa gttatgaaac atataccgtt aaaaaaaatc tgttgcctat
     1681 acagtgtata gaaaatagtt gtggatcgga agatgaaaat gaaaataaaa cacccgttat
     1741 gtatggagta aataatgaat ttatttcgga acttaaagta acagcatttc ccaatccggt
     1801 tattcatacc ttacaaatac aatcaaatca aaccgtacac gtaattataa aagatgctac
     1861 caatcaagta aaaggtgtat attcaaatat ttctcctaat tctgttagta gtattaatat
     1921 gtcacagtac aaatcaggaa tttattttat aactgtgtat aataaagccc aatcaaaaaa
     1981 attatgtgtt gttaaaaatt aattcttatt gtattttgta tatgtagtat aagttgttgt
     2041 tgtagt
//
LOCUS       DAOTOW010000429         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42643_length_2045_cov_3.383417, whole genome shotgun sequence.
ACCESSION   DAOTOW010000429 DAOTOW010000000
VERSION     DAOTOW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42643_length_2045_cov_3.383417"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..702
                     /locus_tag="P5243_07780"
     CDS             172..702
                     /locus_tag="P5243_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRS19388.1"
                     /translation="MIRELSLKNSPRKVLLDTEAYEFLCKNEELQRIKFFENLRSHSS
                     GYAFFQKNWKQEDGTYECQTIYLHKLIAEEFIQKPKINKRLFVRFNNGNPLDCRMENL
                     EWTSLSNVVRNTDKTVNKYGYRGVVKDRGKYRAVIYFDRKPINLGSFDTAKDAAIAYN
                     KKSLELFGNTRSINEI"
     gene            complement(711..1520)
                     /gene="folP"
                     /locus_tag="P5243_07785"
     CDS             complement(711..1520)
                     /gene="folP"
                     /locus_tag="P5243_07785"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRS19389.1"
                     /translation="MNLELSYPCVMGICNVTPDSFYDGGHYVQEKQALHHIERMIQQG
                     AAIIDIGAYSSRPGSADITIDQEYERLLPIVRNTKKYFSHIPISIDTFRSKIVERLYL
                     EVGDIVVNDISGGTLDSNMMSVCGKYSLPYICMHMKGTPQTMQQYTEYTDVVQDIYSF
                     FETQIQQAKQYGIETIIIDPGFGFAKTLNQNYEIIQQLQVFSNLRVPILVGFSRKSMI
                     YKLCDTTPQEALNGTTVLNTLALTKGASILRVHDVLQAVEVITIWKQTNNV"
     gene            complement(1552..>2045)
                     /locus_tag="P5243_07790"
     CDS             complement(1552..>2045)
                     /locus_tag="P5243_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRS19390.1"
                     /translation="TPGDIGFKPIQTSVGSLGVLVCWDQWYPEAARIMALQGADMLIY
                     PTAIGWEYTDTHEEKQRQLQAWIISQRAHAIANGISVISVNRVGLEPDMSGVTHGIQF
                     WGSSFVAGPQGEILVQASSEKQENIVVDIDLGRTEQVRRIWPFFRDRRIDAYSDILKR
                     YAK"
BASE COUNT          693 a          353 c          299 g          700 t
ORIGIN      
        1 aaattaattt tgtagtttta tcggacaata atgttttgta acttaaaaaa aaaataaaat
       61 aatactctca tcttataagt agttttaatt atatttgttc aacaaaatac aatacgcacg
      121 catcctagct attgattttg atataaatac taacttaatt gaacacttac tatgatacga
      181 gaattatcat taaaaaactc accacgcaag gtgttgttag atactgaagc atatgaattt
      241 ttatgtaaaa atgaagaatt acagcgcatt aaattttttg aaaatttacg aagccattca
      301 agtggttatg ctttttttca aaaaaattgg aaacaagaag acggcactta tgaatgtcaa
      361 actatttatt tacacaaact tatagcagaa gaatttattc aaaaaccaaa aataaataaa
      421 cgcttatttg ttcgtttcaa caatggtaat cctcttgatt gcagaatgga aaatttggaa
      481 tggacaagtt tatcgaatgt tgttaggaac accgataaaa ctgttaataa atatggatat
      541 agaggtgtag taaaagatcg tggaaaatat agagccgtaa tatattttga tagaaaacct
      601 ataaatttag gttcatttga cacagcaaaa gatgctgcta ttgcttacaa taaaaaatca
      661 ctagaattat ttggcaacac tcgaagtatt aacgaaattt aacattcgaa tcagacatta
      721 tttgtttgtt tccaaattgt aattacctca acagcctgca atacatcatg aactcttagt
      781 attgaggctc ccttagttag agcaagtgta ttgagtactg ttgtgccgtt taatgcctct
      841 tgtggtgttg tatcacagag tttatatatc atagattttc ttgaaaaacc aactaatatt
      901 ggtactctta aattcgaaaa aacctgtaat tgttgaatta tttcataatt ttgatttaga
      961 gtttttgcaa aaccaaatcc agggtctata attatagttt caataccata ttgttttgct
     1021 tgctgtattt gtgtttcgaa aaaactatat atatcttgaa caacatcggt atactctgta
     1081 tattgttgca tggtttgcgg agttcctttc atatgcatac aaatatatgg caatgaatat
     1141 tttccgcaca ctgacatcat attagaatca agtgttccac cagaaatatc attaactaca
     1201 atatctccta cttctaaata aagtctctct acaatcttgc ttctaaacgt atcaattgaa
     1261 attggtatat gcgaaaaata tttttttgta tttcttacaa ttggtaataa ccgttcatat
     1321 tcttgatcaa tagttatatc ggcagatccg ggtcgtgatg aataggctcc aatatcaata
     1381 attgcagctc cttgttgtat catacgttct atatgatgta atgcttgttt ttcttgcacg
     1441 taatgtcctc catcataaaa tgaatcgggt gtcacattac aaatacccat tacacaagga
     1501 tacgaaagtt ctaaattcat acaagtataa tcaatactat attgtaatat attattttgc
     1561 gtagcgttta agaatatcgc tataagcatc tattctacga tctctaaaaa aaggccatat
     1621 tctacgcact tgctctgttc tgcctaaatc tatatcaacc actatatttt cttgtttttc
     1681 gcttgatgct tgaacaagta tttctccttg tggcccggca acaaaactac taccccaaaa
     1741 ttgtattcca tgtgttaccc cactcatatc gggttccaaa cctaccctat ttactgaaat
     1801 tacagaaata ccattagcaa tggcatgtgc acgttgtgaa ataatccatg cttgtagttg
     1861 acgttgtttt tcttcgtgcg tatcggtata ttcccatcct atagctgttg gataaataag
     1921 catatctgca ccttgtaatg ccataatacg tgcagcttca ggataccatt gatcccaaca
     1981 caccaaaact cccaaactac caacacttgt ttgaataggc ttaaaaccta tatcacccgg
     2041 agtaa
//
LOCUS       DAOTOW010000430         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42650_length_2045_cov_2.683920, whole genome shotgun sequence.
ACCESSION   DAOTOW010000430 DAOTOW010000000
VERSION     DAOTOW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42650_length_2045_cov_2.683920"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="P5243_07795"
     CDS             <1..319
                     /locus_tag="P5243_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS19391.1"
                     /translation="KGVQICIITKNSKTVELFTPTFVSEFDVPEIIPVSTIGAGDTFN
                     AGIIYALYKYGITYDTLNSTPLSFWNSAIHFAIACATTVCMSLDNYIDKQNTRKLLSI
                     IEH"
     gene            473..1411
                     /gene="mdh"
                     /locus_tag="P5243_07800"
     CDS             473..1411
                     /gene="mdh"
                     /locus_tag="P5243_07800"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRS19392.1"
                     /translation="MKITVVGAGNVGATCANVMAQKELASEIVLLDIKEGFAEGKAMD
                     IMQTAALLNFDTRVIGVTNDYSKTAGSDVVVVTSGIPRKPGMTREDLIGTNAGIVNDV
                     AQSILKYSPNAIIVVISNPMDTMTYLTLKKTGLPKNRVIGMGGILDSSRFKYYLSEAL
                     GAQATDIEGTVIGGHGDTTMIPLTRLATYNGVPVSSILSAEALQKVAADTMVGGATCT
                     KLLGTSAWIAPGAAGAALVESIVRDQKKIFPCCVALDGEYGQKDICIGVPVIIGKDGF
                     EKIVDYKLTADEQAAFAKSADAVRNMNDVLKELNIL"
     gene            complement(1503..>2045)
                     /locus_tag="P5243_07805"
     CDS             complement(1503..>2045)
                     /locus_tag="P5243_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19393.1"
                     /translation="IVSGITVEHTSIQQTNSLTTFSIHVTDDSSISSVVGEFVTTQGI
                     QVLPMQYVGENTYSISFAANQLMLGQTVVRFVITDESGNIQRLNKIFVIEDKSSIAHK
                     ELIKIQIFPNPCNNVITINSEHVITSVTMYNSQGVLVLKKNVIEGKNVELIVQSLPQG
                     VYSVVVNFDSKQVAQSIVKL"
BASE COUNT          723 a          327 c          347 g          648 t
ORIGIN      
        1 aaaaggggta caaatatgta taattacaaa aaacagtaaa acagttgaat tatttacccc
       61 cacatttgta agtgaatttg atgttcccga aataatacct gtaagcacca taggagccgg
      121 cgatacgttc aatgccggaa ttatttatgc cttatacaaa tacggtatta cttacgacac
      181 actaaactct acaccattat ctttttggaa ttctgctata cactttgcaa tagcatgtgc
      241 cacaacagta tgtatgagtt tagataatta tattgacaaa caaaacaccc gcaaattact
      301 gtcaatcata gaacattagg taaagaactt acaataaatc tataatacag cttttaaaat
      361 aatcataaaa aagtatgagt ataatttagc atttagctat atgaaaagcc gaaatactta
      421 attatatttg ttacgatata aaaacattta aaaatcataa aattgtaatc atatgaaaat
      481 aacagtagtt ggggcaggaa acgtaggagc aacatgtgca aatgtgatgg ctcaaaaaga
      541 attagcaagt gaaatagtgt tacttgacat caaagaaggt tttgcagaag gtaaagcaat
      601 ggatataatg caaacagcag cattattaaa ttttgacact cgtgtaatag gtgtaacaaa
      661 cgattacagt aaaactgcag gttctgatgt agtagtagta acttccggaa ttcctcgtaa
      721 accgggtatg acacgcgaag atttaattgg tactaatgca ggaatcgtaa atgatgtagc
      781 acaaagcatt ttaaaatatt caccaaatgc tattatagtg gttataagca accctatgga
      841 taccatgact taccttacgt tgaaaaaaac cggattgcct aaaaatcgtg taataggaat
      901 gggtggaatt cttgatagtt cacgttttaa atattactta agtgaagcct taggagcaca
      961 agctactgat atagaaggta ctgtaatagg tggacatggc gatactacta tgattccttt
     1021 aactcgttta gctacataca atggggttcc tgtaagttca atattaagtg ccgaagcatt
     1081 acaaaaagtt gcagccgaca ctatggttgg tggagctact tgcaccaaat tattaggaac
     1141 atcggcatgg attgctccgg gagctgcagg cgccgcttta gttgaatcta tcgttcgcga
     1201 ccaaaagaaa atattcccat gttgtgttgc tcttgatggt gagtacggac aaaaagatat
     1261 ttgtataggt gtgcctgtaa ttattggcaa agatggtttt gaaaaaatag tagattacaa
     1321 acttactgcc gatgaacaag ctgcttttgc taaaagtgct gatgcagttc gcaatatgaa
     1381 tgatgtatta aaagaactaa atattttata atttcatcat atatatttta aaatacaaag
     1441 gctgccttac gataaggcag cctttttttt attctattaa aaatatttgt ataataacct
     1501 atttacaatt taactatact ctgcgctact tgtttagaat caaaattaac aactacagaa
     1561 tacactcctt gcggtaaaga ttgcactatt agttctacat tcttaccttc aattacgttt
     1621 tttttcaaca ccaatactcc ttgtgaatta tacattgtaa cacttgtaat tacatgttcc
     1681 gaattaatcg ttattacatt gttgcatgga ttaggaaata tttgaatttt tattagttct
     1741 ttatgagcaa tcgatgattt atcttcaatc acaaatattt tattcaatcg ttgaatattt
     1801 ccactttcat cagttattac aaaacgaaca accgtttgac caagcattaa ttgatttgca
     1861 gcaaatgaaa ttgaatatgt attttctcca acatactgca ttggcaaaac ctgaattcct
     1921 tgagttgtta caaattcacc aacaacagat gaaatagaag aatcatcggt aacatgaata
     1981 ctaaatgtag ttaatgaatt ggtttgttga atgctggtat gttctactgt aattccactc
     2041 actat
//
LOCUS       DAOTOW010000431         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_42783_length_2040_cov_3.360202, whole genome shotgun sequence.
ACCESSION   DAOTOW010000431 DAOTOW010000000
VERSION     DAOTOW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42783_length_2040_cov_3.360202"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            507..1631
                     /gene="lpxB"
                     /locus_tag="P5243_07810"
     CDS             507..1631
                     /gene="lpxB"
                     /locus_tag="P5243_07810"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRS19394.1"
                     /translation="MKYYVIAGEASGDLHGANVLREIARNDASAEFRLWGGERMSDAV
                     QTPLVKHYKTYDYMGIVEVIKHLRTILKNIAFCKQDIAQYKPDAILFIDFPGFNLRIA
                     PYAKSLGIKTFYFISPTVWAWKESRVKTIQASVDHMFVELPFVKTFYETRHNYHVDFV
                     GHPLLNSIHAFSPKYTYEQFIQKFTLPQKPIIAVMPGSREYEIRENLLTMQAISHNFS
                     EYEFVIAGMSRFSKEFYTKYITASNVSIVFDATYELLSKADAAIVVSGTATLETALFG
                     VPQVIVYKTSPITFYIAKMVVNLNFLGLPNLIMNEQIVPELLQKDMNSKNLNASLHNV
                     LFSSEVRKQIAEKYAALREKLGNVGAAKRTADLIQKYLHE"
     gene            1624..1986
                     /locus_tag="P5243_07815"
     CDS             1624..1986
                     /locus_tag="P5243_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19395.1"
                     /translation="MNKCEEYTCLCESISIHRYSIESFNELDAQGLEEYIYTKTGTRI
                     PYTKLLELHTTQLPVLHEKERHALAQSCGYIDWNAYTEYVQNPHIEGKPQIFRLFLFV
                     AAFVLGIFCIVQMFRFFI"
BASE COUNT          717 a          314 c          330 g          679 t
ORIGIN      
        1 gatatggtag atgtgccatt tgaacaagtt gcaagttaaa ataaaaatga attatatatt
       61 tgaaagcccg attatgtcgg gctttttttg tgtctgaact cttatttttc accacaaaca
      121 tgaagtctaa aggtaccaaa agtttgaaag tccatatacc ttaaaacata acttatatct
      181 taaaatctca tgtttttaac cacagattat acagattttt acagattctc taaaaggttt
      241 aaagcgtctc aaaagtctga aagtgtcaat gtccctatac tgtacaccgt ataccctccc
      301 tcataactta tgcttcgacg agttcaccaa ccaacttaaa actcaaaact atcttaccac
      361 agattcacag attatataga ttttttacca caaaattcac cgaattactc aaagtagctt
      421 tagctcaaaa ctatttttca tagtatttca aaataagtta gacgagaaaa cagtatcttt
      481 gtaatttaaa atttgttgca ttttttatga aatattatgt tatagcaggc gaagcatcag
      541 gagatttaca cggtgctaat gttttgcgtg aaattgcacg aaatgatgct tcggctgaat
      601 ttagattatg gggtggagaa cgtatgagtg atgcagtaca aacgcctttg gtaaagcatt
      661 ataaaactta cgattacatg ggaatagttg aggtaattaa acatctgcga actatactta
      721 aaaacatagc attttgcaaa caagatatag cacaatataa acccgatgct attcttttta
      781 tagattttcc ggggtttaat ttacgtatag ctccgtatgc gaaatcattg ggaatcaaaa
      841 ctttttattt tatttctcca actgtatggg catggaaaga aagtagagta aaaactatac
      901 aagcttctgt agaccatatg tttgttgaat tgccgtttgt aaaaacgttt tatgaaactc
      961 gacacaatta tcatgtcgat tttgttggac atccattgtt gaattctatt catgcgtttt
     1021 cgcccaaata tacttatgag cagttcattc aaaaatttac attacctcaa aaaccaataa
     1081 ttgctgtaat gcccggaagc agagaatatg aaattcgtga aaatttactg acaatgcaag
     1141 ctatttcgca taattttagt gaatatgaat ttgtaatagc cggaatgtca aggttttcaa
     1201 aagaatttta tacaaaatat ataacagctt ctaatgtttc tatagtgttt gatgcaacgt
     1261 atgaactttt aagtaaagca gatgccgcaa tagtagtttc cggaaccgca actttagaaa
     1321 ccgctttgtt tggagtgcca caagttatag tgtataaaac aagtcctata acattttata
     1381 ttgcaaaaat ggtagttaat cttaattttt taggattacc aaatcttata atgaatgagc
     1441 agattgttcc tgaattattg caaaaagata tgaattctaa gaatttgaat gcatcattac
     1501 acaatgtgtt atttagctcc gaagtacgaa aacaaattgc tgaaaaatat gctgcattgc
     1561 gcgaaaaatt aggaaatgta ggagcagcaa aacgtactgc agatttaata caaaaatatt
     1621 tgcatgaata aatgtgagga atatacctgt ttatgtgaaa gtatatctat tcacagatat
     1681 tccattgagt catttaacga acttgatgca cagggattag aagaatatat ttatactaaa
     1741 accggaactc gaattccata cactaagctt ttagaattgc atactacaca attacccgta
     1801 ttgcacgaaa aagaacgaca tgctttagcg caatcatgtg gttatattga ttggaatgcg
     1861 tatactgaat atgttcaaaa tcctcacatt gagggtaaac cacaaatatt tagattgttt
     1921 ctatttgttg cagcttttgt gttgggaata ttttgtattg ttcaaatgtt tcgatttttt
     1981 atttaataat cattattgtc cgataaaact acaaaattaa tttaataaaa caaaatttat
//
LOCUS       DAOTOW010000432         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43034_length_2031_cov_3.357287, whole genome shotgun sequence.
ACCESSION   DAOTOW010000432 DAOTOW010000000
VERSION     DAOTOW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43034_length_2031_cov_3.357287"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="P5243_07820"
     CDS             <1..884
                     /locus_tag="P5243_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19396.1"
                     /translation="PPLFNYYIALKNKHFYIGLFSFLLVVVSYYLIRELYNPGYLKAV
                     QQNELGGRYLEALENNKKSFWYYYTNFIDFQLTAWYILIPCGLATGFFIKNQKINRLT
                     LFSFLMIITFFIVISFAQTKLEWYDVPLYPFIAIVIGIFIFYVFDLLQKNIIINQTLT
                     VNVIPYVFLFILGITPYQKIIAKTYKPKEYSWHKNFYEISYYLQDAVKNKHDINNQFI
                     VYNGYNAHILFYVKKLNDKGISISFKDWKFLEPKDIVIVSQNKIKQYIEEHYINEIIQ
                     KNGNILTYKIYERKNAN"
     gene            862..1806
                     /locus_tag="P5243_07825"
     CDS             862..1806
                     /locus_tag="P5243_07825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS19397.1"
                     /translation="MKEKMQIEVSVIIPIYNEQEIIPELYSRLQKSVSKISQNYELIF
                     VNDGSKDASIQELKKIANTDSHVLYINFSRNFGHQIAVTAGLHHATGNAIVIIDGDLQ
                     DPPELIPELYAKYKEGYKIVGAKRVSRKGETFFKKVTAKAYYRILKRITNVDIPVDTG
                     DYRLIDRKVVQAILAMDEPDKYIRGQVAWTGFKTTFVNFERDERRFGTTGYSLKKMTK
                     FAIDGITSFSSYPLKLASIFGFIVSGFAFCMVVYVLLSKYVFQTAVEGWPSLMLTILF
                     LGGVQLICLGIIGEYLWRIMNTVRNRPLYIIEETNIES"
     gene            1806..>2031
                     /locus_tag="P5243_07830"
     CDS             1806..>2031
                     /locus_tag="P5243_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19398.1"
                     /translation="MIIGKQLISTLKNGIASNIYVFAIIIIALVAYWNIASCNYFLKY
                     DIIDANFPNQVFQSQLEQQGEIPLWDMFSYF"
BASE COUNT          716 a          236 c          335 g          744 t
ORIGIN      
        1 taccacctct ttttaattat tatatagctt taaaaaataa acatttttat ataggcttgt
       61 tttctttttt attggtagta gtatcgtatt atcttatcag agaattatat aatcccggat
      121 atcttaaagc ggtacaacaa aatgaattag gagggagata tttagaagct cttgaaaata
      181 ataaaaagtc tttttggtat tattatacta attttattga ttttcaatta acagcttggt
      241 atattttaat tccttgtggg ttggctactg gttttttcat taaaaatcaa aaaattaatc
      301 ggttaaccct gttttctttt ttgatgatta ttactttttt cattgtaatt tcttttgctc
      361 aaactaagct tgaatggtat gatgtgccat tatatccttt tatagcaatt gtaattggaa
      421 tttttatttt ttatgtgttc gatttgttac aaaaaaatat tattattaat caaactctta
      481 ctgtaaatgt aattccgtat gtttttttgt ttattttagg aattacacca tatcaaaaaa
      541 ttattgctaa aacatataaa ccaaaagaat atagttggca caaaaatttt tatgaaatta
      601 gttattattt gcaagatgct gtaaaaaaca aacatgatat aaataatcaa tttattgtgt
      661 ataacgggta caatgctcat attttatttt acgtaaaaaa attaaacgat aaagggattt
      721 caatatcatt taaggattgg aaatttctag aaccaaagga tatagtaata gtaagtcaaa
      781 ataaaataaa acaatatatt gaagaacact atataaatga gataattcaa aagaatggaa
      841 atatattaac gtataaaata tatgaaagaa aaaatgcaaa ttgaggtttc tgtaattatt
      901 cctatatata atgaacaaga aataattcct gaattatata gtagacttca aaaaagtgta
      961 tcgaaaatat ctcagaatta tgaattaatt tttgtaaacg atgggagtaa agatgcatcg
     1021 atacaagaat tgaaaaaaat tgcgaacacc gattcgcatg ttttgtatat taattttagt
     1081 cggaattttg ggcatcaaat agctgttact gccggattac accatgctac aggtaatgct
     1141 attgttatta tagatggcga tttacaagat cctcccgaat taatcccgga attgtatgca
     1201 aaatataaag aaggatataa aattgtaggg gccaagcgag tgtcacgcaa aggtgaaacg
     1261 tttttcaaaa aggttactgc aaaggcatat taccgtatac tcaaacgtat taccaatgtg
     1321 gacatacctg tagacacggg tgattataga ttaatagata gaaaagttgt tcaagcaatt
     1381 cttgctatgg atgaacccga taagtatatt cgaggtcagg tagcatggac aggttttaaa
     1441 actacatttg ttaattttga acgcgatgaa cgtagatttg gcacaaccgg atattcttta
     1501 aagaagatga ctaaatttgc aatagatggc attacatcgt tttcgagtta tcctttgaaa
     1561 ttagcatcga tatttggatt tattgtttcg gggtttgctt tttgtatggt tgtatatgtt
     1621 ttgttatcaa agtatgtttt tcaaacggct gttgaaggtt ggccatcgtt aatgcttact
     1681 attttgtttt tgggcggagt tcaattaatt tgtttgggta ttattggaga gtatttatgg
     1741 agaattatga atacggtacg taatagacca ttatatataa ttgaagaaac caatatagaa
     1801 tcataatgat tataggtaaa caattaattt ctacattaaa aaacggtatt gcttcaaata
     1861 tatatgtgtt tgcaattatt ataattgctt tagttgctta ttggaatatt gcatcatgta
     1921 actatttttt aaaatatgat atcatagatg caaattttcc taatcaagta tttcaatcgc
     1981 agctcgaaca acaaggtgaa ataccgttgt gggatatgtt ttcttatttt g
//
LOCUS       DAOTOW010000433         2027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43160_length_2027_cov_3.095842, whole genome shotgun sequence.
ACCESSION   DAOTOW010000433 DAOTOW010000000
VERSION     DAOTOW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43160_length_2027_cov_3.095842"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="P5243_07835"
     CDS             <1..527
                     /locus_tag="P5243_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRS19399.1"
                     /translation="PISDGKRIFSIVTNLEKATKTEGKELVRSFLKTIILVSIDEAWK
                     EHLRELDDLKQSVQNASYEQKDPLLIYKFESFNLFQTMLETVNKEVVSSVLKAHIPIQ
                     AEAHEAQERKRMPNNLQAKKDDANTATRNQDTREQPKSAPVRVGPKVGRNDDCPCGSG
                     KKYKNCHGKSSIDD"
     gene            502..1074
                     /locus_tag="P5243_07840"
     CDS             502..1074
                     /locus_tag="P5243_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19400.1"
                     /translation="MENLLLMTNIVNMIEQECAISSDQISNYVKRMLSKRLKIVHIGF
                     SIVSIIFIFMSLLSKSLLFFVLGAIILGFVYIYIRAGFTYEIQKIGKGLRIVISNSSV
                     RKFIENESLQSIKQSKRTQTAYSYTQEIPFSDITSITIKESEIIIFTKQANTQNEYGR
                     ITIPCEIESFENISDYFNSNPDKFPRINRK"
     gene            1426..>2027
                     /locus_tag="P5243_07845"
     CDS             1426..>2027
                     /locus_tag="P5243_07845"
                     /inference="COORDINATES: protein motif:HMM:NF036305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19401.1"
                     /translation="MTNLTKNQKLIKWVNEWAEICQPDSVYWVDGSEEENNRLNDMMV
                     ASGAAVKLNDKKRPNSYYYQSDPSDVARVENRTYICTEKEEDAGPTNNWMAPKEMKEI
                     LLAKYKGCMKGRTMYVIPFSMGPLGSNIAKIGVEITDSAYVVVNMRIMTRMGKAVLDV
                     LGDGDFIPCVHSIGAPLAPGQKDVAWPCAPIEEKYISHFP"
BASE COUNT          825 a          307 c          311 g          584 t
ORIGIN      
        1 ttcctatctc cgatggtaaa cgtatattta gtattgtaac aaatttagaa aaagcaacaa
       61 aaaccgaagg aaaagaatta gttcgttcat tcttaaaaac tataattctt gtaagtatag
      121 atgaagcttg gaaagaacac ttacgagaat tagacgattt aaaacaatcg gttcaaaatg
      181 cttcatatga acaaaaagat cctctgttaa tttataaatt tgaatcattt aatttatttc
      241 aaaccatgct tgaaacggta aataaagaag tagtttcaag tgttttaaaa gcacatattc
      301 ctattcaagc cgaagcgcat gaagcacaag aacgaaaaag gatgccaaac aacttacaag
      361 caaaaaaaga tgatgcaaat acagcaactc gtaatcaaga cactcgagaa caaccaaaat
      421 cagcaccggt aagagttggt cccaaagtag gcagaaatga tgattgccct tgcggaagtg
      481 gcaaaaaata taaaaattgt catggaaaat cttctattga tgactaatat tgtaaatatg
      541 atagaacaag aatgtgcaat atcatctgac caaatatcta attatgttaa aagaatgctt
      601 tccaaacgat taaaaatcgt tcatatagga tttagcattg tttctatcat tttcatattc
      661 atgtcgctcc tttctaaatc attgctgttt tttgtgttgg gagcaatcat tttaggattt
      721 gtatatatct atataagagc aggttttaca tacgaaatcc aaaaaattgg taaagggctg
      781 cgtatagtta tatcaaactc ttctgttcgc aaattcattg aaaatgaatc attacaaagt
      841 ataaaacaat caaaaaggac acaaacagcc tatagttata cacaagaaat tcctttttct
      901 gatattacat caattacaat aaaagaatct gaaataatta tttttacaaa acaagcaaac
      961 acacagaatg aatacggtag aataactata ccctgtgaaa tagaatcatt tgaaaatata
     1021 tcagattatt ttaattcaaa tcccgataaa tttccccgaa taaacagaaa ataaatgcta
     1081 agtatcatta taatgtatag taaacaatcc tcattgtaaa ctacaaattt tatttttctg
     1141 aaaagccaaa aatattcatt ctaaaatcta ctgatatatt acatgaattt ttatgagtat
     1201 tcatacaaaa aaacaaaaat atctaaaaag aaaaaaacat aaaatactaa aatacaaaaa
     1261 gttaaacata aattaaaaaa ctataaacac ataaaaacac gaagtaaaat tgatactata
     1321 gtcaataagc taattttgaa attgaagaat tttatctatt tttgaagtaa tgtttgaaac
     1381 ttagaaaaca taaaaaaatt aatattaact aaacgtttta caaagatgac aaatttaacg
     1441 aaaaatcaaa aactcattaa atgggtaaac gaatgggcag aaatttgcca accggattca
     1501 gtttattggg ttgacggatc tgaagaagaa aataatcgct taaacgacat gatggttgca
     1561 agcggtgctg cggtaaaatt aaacgacaaa aaacgtccta attcttacta ctatcaatca
     1621 gacccaagtg acgttgcacg tgtagaaaac agaacgtaca tttgtactga aaaagaagaa
     1681 gatgccggac caacaaacaa ctggatggct ccaaaagaaa tgaaagaaat tctattggca
     1741 aaatacaaag gttgtatgaa aggtcgtact atgtatgtta tcccattcag catgggtcca
     1801 ttaggttcta acattgcaaa aattggtgta gaaataactg actctgcata tgttgttgta
     1861 aacatgagaa tcatgactcg tatgggtaaa gctgtacttg atgtattagg tgatggcgat
     1921 ttcattccat gtgttcactc tattggtgct ccattagctc cgggacaaaa agatgtagca
     1981 tggccatgtg ctccaattga agaaaaatat atttctcact tcccaga
//
LOCUS       DAOTOW010000434         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43183_length_2026_cov_4.253171, whole genome shotgun sequence.
ACCESSION   DAOTOW010000434 DAOTOW010000000
VERSION     DAOTOW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43183_length_2026_cov_4.253171"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="P5243_07850"
     CDS             <1..1265
                     /locus_tag="P5243_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRS19402.1"
                     /translation="SYLLDTIIETIPAPKPNPGTLQMRITSLDYSSYVGRIAVGRVSR
                     GSIIAGQNISLVKRDGTIVKSKIKELFVFEGLGKEKVKTEITAGEICAVMGIEGFDIG
                     DTIADFENPEALANISIDEPTMSMIFTNNTSPFFGKEGKFVTSRHIRERLYKELERNL
                     ALRIEETESPDMLLVYGRGILHLSVLIETMRREGYELQVGQPKVIIKEIDGVKHEPIE
                     HATVHVPEPFAGKVIELMTQRKGELQNMTTKNDRVHLEFTIPSRGLIGLTNAILTATE
                     GEAVLSHRFLGFEPWKGDMPSKINGALIAMETGPTFAYAIDKLQDRGKFFIYPGEDVY
                     AGQVIGEHIRDNDLIINVGKSKKLTNMRASGADDKTSIAPPIRMSLEEHMEYIRKDEF
                     LEITPKSLRIRKIILNEHDRKRENNKIG"
     gene            1338..>2026
                     /locus_tag="P5243_07855"
     CDS             1338..>2026
                     /locus_tag="P5243_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS19403.1"
                     /translation="MEKAQYEEELVKLAQEGKLQELLELSNKLVDEYPSWHIAWHYLA
                     VAYGLQNDHERAISYFIKVLELKPHSVKALYGLSVSFHALKMYEEAISCLLKHKELHG
                     SEYKILFGLGVNYTELNMLPQAIDAFTEALTFEPQNEETLFCLADCYRMQENYAKAIE
                     LYLQSLDINPQNILVIHNTAVSYKYLNNNEKAIEFYLKVLSLDPAFFRSLRNLAEIYI
                     EMNQPQLAKPL"
BASE COUNT          738 a          289 c          383 g          616 t
ORIGIN      
        1 ttagttattt gctcgataca attattgaaa ctattcctgc accaaaacct aatccgggaa
       61 cattgcaaat gcgaataaca tcattagatt attcatcata tgtaggcaga attgcagttg
      121 gcagggtatc tcgcggtagt ataatagccg ggcagaatat atcattggta aaacgtgatg
      181 gtacaattgt aaaatctaaa attaaagaat tatttgtatt tgaagggttg ggtaaagaaa
      241 aagtaaaaac cgaaataact gcaggcgaaa tatgtgcggt tatgggaata gaaggatttg
      301 atataggtga tacaattgca gattttgaaa atcccgaagc tcttgcaaat atttcaatag
      361 atgaaccaac aatgtctatg atttttacaa ataatacgtc tccatttttt ggaaaggaag
      421 gtaaatttgt aacttcacga catattcgag aacgattgta taaagaatta gaacgaaatc
      481 ttgctctccg aatagaagaa acagaatctc ccgatatgtt gttggtttat ggtagaggta
      541 ttttgcatct ttcggttctt atagaaacta tgcgacgcga gggatatgaa ctacaagtgg
      601 gtcaaccgaa agtaattata aaagaaatag acggagtaaa acatgaacct atagaacatg
      661 caacagtgca tgttcccgaa ccgtttgccg gaaaagtaat agaactaatg acccaacgca
      721 aaggtgaatt gcaaaatatg acaacaaaaa atgatagagt acatttagag tttaccatac
      781 catcgcgtgg attgataggg ttaactaatg ctatacttac tgctaccgaa ggtgaagctg
      841 ttctgtctca tcggtttttg ggttttgaac catggaaagg tgatatgcca agtaaaataa
      901 atggggcttt aatagctatg gaaacaggac caacttttgc ctatgcgatt gataaactgc
      961 aagatagagg aaaattcttt atttatccgg gcgaagatgt atatgcaggt caagtaatag
     1021 gtgaacatat acgagacaat gatttaatta taaatgtagg aaaaagtaaa aaactcacta
     1081 atatgcgggc ttcaggagct gatgataaaa caagcattgc tcctcctata cgtatgtcgc
     1141 ttgaagaaca tatggaatac attagaaaag atgaattttt ggaaattacc ccgaagtctc
     1201 tgagaataag aaaaataata ttaaatgagc atgatagaaa acgcgaaaac aataaaatag
     1261 gataattttt atgtatttgt gtaacacttt ttttatttga tatgtacatt tgtaaagtaa
     1321 ttgtttaaat ttacgatatg gaaaaagcac aatatgaaga agagttagta aaattggctc
     1381 aggaaggtaa attacaagaa ttacttgaat tgtctaacaa acttgtagat gaatatcctt
     1441 cgtggcatat tgcatggcat tatttggctg tggcatatgg cttacaaaac gaccacgaac
     1501 gagcaatttc atatttcatt aaagttcttg aattgaaacc tcattcggta aaagctctct
     1561 atggtttaag tgtttctttt catgcgctta aaatgtatga agaagcaata tcgtgtttac
     1621 tcaaacacaa agaattacat ggtagcgaat ataaaatatt atttggattg ggtgtaaatt
     1681 atacagaact caatatgctt cctcaagcaa ttgatgcatt tactgaagct cttacctttg
     1741 aaccacagaa cgaagaaaca ttgttttgtt tggctgactg ttatagaatg caagaaaatt
     1801 atgcaaaagc aatagaatta tacttacaat cattagatat taatcctcaa aatattttgg
     1861 taattcataa tacggctgtt tcttataaat atttgaataa caatgaaaaa gcaattgagt
     1921 tttatttgaa agttctttct ttagatcctg catttttccg ttcattacga aatttggccg
     1981 aaatttatat tgaaatgaat caaccacaat tagcaaaacc acttat
//
LOCUS       DAOTOW010000435         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43191_length_2026_cov_3.126839, whole genome shotgun sequence.
ACCESSION   DAOTOW010000435 DAOTOW010000000
VERSION     DAOTOW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43191_length_2026_cov_3.126839"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="P5243_07860"
     CDS             complement(<1..279)
                     /locus_tag="P5243_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19404.1"
                     /translation="MIEWLEHIDQQLFLFLNGLHSPFFDPIMYYASKFYISLPLYAYI
                     IITLYRTKSIKQATLIFTLMILCVALADIISVAWFKEVFLRYRPSHNRN"
     gene            complement(276..1268)
                     /gene="obgE"
                     /locus_tag="P5243_07865"
     CDS             complement(276..1268)
                     /gene="obgE"
                     /locus_tag="P5243_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRS19405.1"
                     /translation="MSSSNFVDYIKIFFRSGNGGNGSAHLHRDKLTAKGGPDGGDGGR
                     GGHIILRGNENVWTLLALRYQKHIFAGHGGDGGSSGKTGAYGEDIYVDVPLGTIARNA
                     ETNEFLFEITAHGEEKILLQGGRGGQGNMHFKSATNQTPRFAQPGESGIEGWYALELK
                     VLADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLVPNLGIVSYRNNASFVMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSVLLFMIPADSKSITLEYNILLNEVTMYNPELLDK
                     PRLLAITKCDMIDEELQRMLQAELPPIDYVFISSVSQYNISLLKDKIWNLLHSI"
     gene            complement(1284..1859)
                     /locus_tag="P5243_07870"
     CDS             complement(1284..1859)
                     /locus_tag="P5243_07870"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRS19406.1"
                     /translation="MLNIVLFGPPGAGKGTQSDLIVEKFKLIHISTGDLLRAEISKQT
                     NLGKQAQDIINKGLLMPDSIMIDMIQNKITEHIGCKGFIFDGFPRTTVQAEALDSLLR
                     QNNMEISTMISLEVDTPIIVERILKRGAISGRSDDKNETIILQRISEYNHKTAPVKEF
                     YKKCNKLTEIQGVGTIQEIFSKIEKVCSTFV"
BASE COUNT          625 a          369 c          317 g          715 t
ORIGIN      
        1 atttctgttg tgtgatggac ggtaccgtaa aaaaacttct ttaaaccacg caacagaaat
       61 tatatcggct aaagcaacac atagtatcat aagtgtaaat atgagtgttg cttgttttat
      121 tgatttagtt ctatacagtg taataataat atatgcatac aatggtaaag aaatataaaa
      181 ctttgatgca taatacatta taggatcgaa aaatggagaa tgtaacccat ttaaaaataa
      241 aaataattgt tgatcaatat gttctaacca ttctatcata tgctatgtaa taggttccaa
      301 attttatctt ttaaaagaga aatattatat tgcgataccg aagatataaa tacataatca
      361 ataggaggta gttctgcttg taacatacgc tgtaattctt cgtcaatcat atcgcattta
      421 gttatagcca ataatctcgg tttgtcaagc aattccggat tatacattgt tacttcattt
      481 aataaaatat tatattccaa tgtaatactt tttgaatcgg ccggtatcat aaatagtaat
      541 acagaattgc gttcaatatg acgtaaaaat cgaagaccta aacctttgcc ttcatgggcg
      601 ccttctatta ttcccggtat gtctgccatt acaaacgatg cattgtttct gtatgaaaca
      661 atacctaaat ttggaactaa tgtggtaaat ggataatcag caatttttgg tttagctgca
      721 gaaacaacgc ttagtagtgt agatttaccg gcattaggaa acccaacaag gcctacatcg
      781 gcaagtactt taagttctaa agcataccaa ccttctatac cactttcgcc aggttgtgca
      841 aatcgcggag tttgattagt tgccgattta aaatgcatgt tgccttgtcc gccacgtcct
      901 ccttgaagta aaattttttc ttcgccatga gccgttattt caaataaaaa ttcatttgtt
      961 tctgcattac gggctatagt gcctaaagga acatctacgt atatgtcttc accgtaggca
     1021 cctgtttttc cggaactacc accatcacca ccatgtccgg caaatatgtg tttttggtaa
     1081 cgtaatgcta acagagtcca tacattttca ttccctcgta aaattatatg accacctctg
     1141 ccaccatcac ctccatcagg gccaccttta gctgtcagtt tatctctgtg taaatgtgcc
     1201 gaaccgtttc ctccattacc tgaacggaaa aatattttaa tataatctac aaaatttgat
     1261 gatgacatgt atgtaagtat tttttataca aaagtactgc aaactttttc aattttacta
     1321 aaaatctctt gaattgtacc tactccttgt atttcggtaa gtttgttgca ttttttgtaa
     1381 aattctttta ccggagctgt tttgtgatta tattctgaaa tgcgttgtaa tattattgtt
     1441 tcatttttgt catcggaacg gcctgaaata gcaccgcgtt ttaatatgcg ttctactatt
     1501 attggggtat ctacttcaag cgaaatcatt gttgaaatct ccatgttgtt ctgtctcaac
     1561 aatgaatcaa gtgcttctgc ttgaacagta gtgcgcggaa atccatcaaa aataaaacct
     1621 ttgcagccga tatgttctgt aattttattt tgaatcatat caatcataat agaatcaggc
     1681 attagtaatc ctttgtttat aatgtcctga gcttgcttac ctaaattagt ttgttttgaa
     1741 atttctgcac gtaacaaatc tccggttgaa atgtgaatta atttaaattt ttctacaatt
     1801 aaatcggact gcgttccttt tcctgctccc gggggtccga ataatacaat atttagcatg
     1861 ttttataatt taatgtgtct gtaatacata tatttccttc aaatttctgc caattccatt
     1921 ataatccatt ccatatccta ctacaaaatt attaggaata gtaaatccgg taaattgaat
     1981 tgctgccttc cctttatatg cgtttggttt aaatactaat gctgct
//
LOCUS       DAOTOW010000436         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43358_length_2020_cov_3.897201, whole genome shotgun sequence.
ACCESSION   DAOTOW010000436 DAOTOW010000000
VERSION     DAOTOW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43358_length_2020_cov_3.897201"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="P5243_07875"
     CDS             <1..249
                     /locus_tag="P5243_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19407.1"
                     /translation="GGIEQNILFFNQHFFTKSNYQTEYKEYETYIYRTYCIQSFSFVK
                     SNFPTWNIGGIFGAQFIVPKTDIDYTRLHMSININYSF"
     gene            268..795
                     /locus_tag="P5243_07880"
     CDS             268..795
                     /locus_tag="P5243_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19408.1"
                     /translation="MKSQHIFIFILLSLCISCGTTECPPFPDELQGYVPYQLHQKIQF
                     YNENNDTVTLLVTEASVSSPSSFKNNCDCECYTIKKIKTNKDSKFNINCNIEFSVFSE
                     TNNNIMLYFYINTNYGYFTKNTIQYSKSILPLGDTIHIEKYSGTDFSNMKIVYNKGLI
                     QFYDTRNTCLWKLVE"
     gene            complement(1107..1703)
                     /gene="leuD"
                     /locus_tag="P5243_07885"
     CDS             complement(1107..1703)
                     /gene="leuD"
                     /locus_tag="P5243_07885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRS19409.1"
                     /translation="MAIEKFTTTISRVVPIPIEEIDTDQIIPARFLKATDKKGFGDNL
                     FRDWRYDKNNQPIADFPLNNPKYSGCILVGGKNFGCGSSREHAAWAIADYGFKVVVSS
                     FFADIFKNNALNNGVLPVQVSEAFLAKIFAAVDTNPATELKVDLENQTITLLATNESE
                     HFDINAYKKDCLLNGFDDIDYLISKLPEIEAFEKKYKS"
     gene            complement(1761..>2020)
                     /locus_tag="P5243_07890"
     CDS             complement(1761..>2020)
                     /locus_tag="P5243_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRS19410.1"
                     /translation="VEKQIKEEGLDKIFAEADVELRQPGCSSCLAMNEDKVPPGKYCV
                     ATSNRNFEGRQGPGARTILASPLTAAAAIITGKITDPREFL"
BASE COUNT          656 a          324 c          314 g          726 t
ORIGIN      
        1 ggtggtatag agcaaaatat tttatttttt aatcaacatt tttttacaaa aagcaactat
       61 caaactgaat ataaagaata tgaaacatac atttatagaa catattgcat acaatctttt
      121 tcttttgtaa aatcaaattt tcctacatgg aatattggag gtatatttgg tgctcaattt
      181 atagttccta aaacagatat agattatact cgattacata tgtcaattaa tataaattat
      241 agtttttaat atcggaaacc ataaacaatg aaatcacagc atatttttat cttcatatta
      301 ctcagcttat gtatttcctg cggcacaaca gaatgtcctc catttcctga tgaattgcaa
      361 ggatatgtac cctatcaatt acatcaaaaa atacagtttt ataatgagaa taacgacacg
      421 gttacacttt tagttactga agcttcggta agtagccctt catcatttaa aaacaattgc
      481 gattgcgaat gttatacaat taaaaaaata aaaactaata aagattcaaa atttaatatt
      541 aattgtaata ttgaattttc tgtttttagt gaaactaata ataatataat gttatatttt
      601 tatattaata ctaattatgg ctatttcaca aaaaatacta tacaatattc caaatcaata
      661 ttgcctttag gtgataccat acatattgaa aaatatagtg gtacagactt ctcaaatatg
      721 aaaattgtgt ataataaagg gctcattcaa ttttacgata ctagaaatac ctgtttgtgg
      781 aaattggtgg aataacacta tagtattact ttcattatag tcttcgtttc atcattatgt
      841 gcactattat tttcctgcca tgatgagttt tttagtatat ttgtgagttt gttgaaccaa
      901 acgttgagtc tgtgtaactt tgtgaccttt tcgctaaagc taaacaatgt gtagaaaaaa
      961 atctttgaaa attcgtgtaa ttcgccaagg cggaacaagt atgtggtaaa aaaatagaat
     1021 ataaaaggca acccaacgga ttgcctctcc aaatcaatat ttgtgtctta gtgccttcgt
     1081 ggcaaaagag tataccacaa tacctattaa cttttatact tcttttcaaa tgcctctatt
     1141 tcaggtaatt tgcttattag atagtctata tcatcaaatc cgttgagcaa acaatctttt
     1201 ttgtaagcat ttatatcaaa atgttccgat tcgttggttg ctaataaagt aattgtttga
     1261 ttttctaaat ctactttgag ttcggtagca gggtttgtat caactgcagc aaatatttta
     1321 gctaaaaatg cttcggaaac ttgtaccggt agtacaccat tgttaagagc attgtttttg
     1381 aaaatatcgg caaaaaagct tgatactact actttgaagc catagtctgc aatagcccat
     1441 gctgcatgtt cgcggctcga tccgcagcca aagtttttgc cacctaccaa tatacaacca
     1501 ctgtattttg ggttatttaa cggaaaatct gctattggtt ggttattttt gtcgtatctc
     1561 cagtcgcgga ataaattgtc gccaaaacct tttttgtcgg ttgctttcaa aaaacgagca
     1621 ggtataattt ggtcagtatc tatttcttct attggaatag gtactacacg tgatattgtt
     1681 gttgtaaatt tttctattgc cattgatata atttttaatt ataaattatt aatttttaat
     1741 tgtttgagtt tcactcaagt ttataaaaat tctcgtgggt cggttatttt tccggtaata
     1801 attgcagcag cagcagtaag cggacttgca agtatggtgc gagcacccgg accttgacga
     1861 ccttcgaagt tgcggtttga cgttgctaca caatatttac cgggaggtac tttgtcttcg
     1921 ttcattgcca aacaacttga acaacccggt tgacgaagtt ctacatcagc ttcggcaaat
     1981 attttatcaa gaccttcttc tttaatttgt ttttcaacca
//
LOCUS       DAOTOW010000437         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43517_length_2014_cov_3.752425, whole genome shotgun sequence.
ACCESSION   DAOTOW010000437 DAOTOW010000000
VERSION     DAOTOW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43517_length_2014_cov_3.752425"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="P5243_07895"
     CDS             <1..1346
                     /locus_tag="P5243_07895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRS19411.1"
                     /translation="GTNDSQKSIISPLAIILFALHTIVGFLPLQEGSLFGGMFNTTNI
                     VATMKNILNLGVLIILLQSWKWLNATENRVKVSEFYIVLFSTLLGMFYMISAGDFLMF
                     YIGLEIATIPLAALAAFNRHENKSAEAGIKLLLSSALSSGILLYGISMIYGTTGSIYF
                     AEISQSFKGFEPIQVMAMIFFFSGMAFKISLVPFHLWTADVYEGAPVSITSYLSVISK
                     GAAAFVFMIILFTVFKLFTPVWQDMMTAIAILTMTIGNLFALRQNNLKRYLAFSSIAQ
                     AGFILLGIISGTATGMATVVYFILIYIFSNLSAFGVVSIISAYTGKDSISDLNGMYKT
                     NPKLSLALTLALFSLAGIPPVAGFFGKFFLFTAAAEKDFYILVLIALLNTIVSLYYYL
                     MPVKAMFINKSESPIAYFKTDKISQLGLAISIIGMVVIGFASPVYEKILSFCFGI"
     gene            1359..1724
                     /locus_tag="P5243_07900"
     CDS             1359..1724
                     /locus_tag="P5243_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19412.1"
                     /translation="MALTNGKHIVKELNGTPCTIIEEQVSQSRANFLKSLLTFNKLEV
                     IVVANPATEGAEETYTIGVTDVIFNPVIAVYAKKLYTQHGRIVTPNIWNQIPENQHVP
                     YWTIGRQSIEIYSEELQKS"
     gene            1798..>2014
                     /locus_tag="P5243_07905"
     CDS             1798..>2014
                     /locus_tag="P5243_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS19413.1"
                     /translation="MQIQTFVFNHFGQNTYLVIDEQTKECAVIDPGCFFDEEKQKLIS
                     YIQTNTYNLTKIVFTHCHLDHAFGARSI"
BASE COUNT          644 a          330 c          336 g          704 t
ORIGIN      
        1 ttggcaccaa tgattcgcaa aaatcgataa tatcaccact tgcaattatt ttatttgctc
       61 ttcacaccat agttggattt ttaccattgc aagaaggtag tttgttcggc ggtatgttca
      121 atacaacaaa catagttgct actatgaaaa atatattgaa cctcggtgtt cttattattt
      181 tacttcaatc atggaaatgg cttaatgcta ctgaaaaccg agttaaggta agtgaatttt
      241 atatcgtatt attctcaaca ttacttggta tgttctatat gatatctgcc ggtgattttc
      301 ttatgtttta cataggatta gaaattgcga ctataccatt agctgcattg gctgcattca
      361 atcgtcacga aaataaatct gctgaggcgg ggattaaatt attattatct tcagctttgt
      421 catcgggaat tttactttat ggaatttcta tgatatacgg aactaccggt tctatttatt
      481 ttgccgaaat ttcacaatct ttcaaaggtt ttgaaccgat acaagtaatg gcaatgatat
      541 tcttcttttc gggcatggca tttaaaattt cattggttcc attccattta tggactgccg
      601 atgtgtatga aggtgcaccg gtaagtatta catcatattt atctgtaatt tcaaaaggcg
      661 ccgcagcatt tgtgtttatg attatactgt ttaccgtttt caaactattt accccggtat
      721 ggcaagatat gatgacggct atagcaattt taacaatgac cataggtaat ttatttgcat
      781 tgcgtcagaa taatttaaaa cgatatttgg cattttcatc aattgctcaa gcagggttta
      841 ttctgcttgg aataatatca ggaactgcta ccggtatggc taccgtagta tattttatat
      901 taatatatat attctcaaat ttatcggctt ttggtgttgt gtctattatt tcggcatata
      961 ccggcaaaga cagtatttct gatttaaacg gcatgtacaa aaccaatcca aaattaagtt
     1021 tggctttaac acttgctttg ttttcgcttg caggtattcc gcctgttgcc ggattttttg
     1081 gtaaattctt cttatttacc gctgctgcag aaaaagattt ttatattctc gtattaattg
     1141 cattactcaa tactatcgta tcattatact attatttaat gccggtaaaa gcaatgttta
     1201 ttaacaaaag cgaatcgcct attgcgtatt tcaaaacaga taaaatttcg caattaggtt
     1261 tggctattag tattatagga atggttgtaa taggatttgc aagtcctgtt tacgaaaaaa
     1321 ttcttagttt ttgttttgga atttaaattt gtacaattat ggcacttact aacggaaaac
     1381 atatagtaaa agaactaaat ggtaccccat gtactattat tgaagaacaa gtttcgcaat
     1441 cacgtgcaaa ttttttgaaa tcattgctta cttttaacaa actcgaagtt attgttgtag
     1501 caaacccggc aacagaaggg gcagaagaaa catacaccat tggagtaacc gatgttatat
     1561 tcaatcctgt aattgctgta tatgcaaaaa aattatatac gcagcacgga cgaatagtta
     1621 cccctaatat atggaatcaa attcctgaaa atcaacatgt accatattgg actattggca
     1681 gacaatcaat agaaatttat tctgaagaat tacaaaaatc ataataaaac agggaattag
     1741 tcattatcta taagccttag ctccaaattc tgtatatttg cataaaaaga tacatctatg
     1801 caaatacaaa catttgtgtt taatcatttt ggacaaaata cctatcttgt aattgatgag
     1861 caaacaaagg agtgtgcggt aattgacccg ggttgttttt tcgatgaaga aaaacaaaaa
     1921 cttatttcat atatccaaac caatacgtat aacctaacta aaattgtttt tacacattgt
     1981 catctcgacc atgcatttgg agcacgcagt attg
//
LOCUS       DAOTOW010000438         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43641_length_2010_cov_2.891560, whole genome shotgun sequence.
ACCESSION   DAOTOW010000438 DAOTOW010000000
VERSION     DAOTOW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43641_length_2010_cov_2.891560"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="P5243_07910"
     CDS             <1..571
                     /locus_tag="P5243_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19414.1"
                     /translation="VAFSNSQWIDEYGHSIKRRTTKTWSRDFTMNGIDFVKKYLLGYN
                     YICNASAVLFKRTALTAVTNDYTQYKASGDRLFWIQICLQGKVCYVAEKLNFFRQHTH
                     KVSGHAAETGVNMVEDFSIYQKIKTLVPFNFWEQSLICGYHYHSIHYCQQFVGNGKQF
                     ALSAWEQTRFFNMFSYITYLFFRAIEIVR"
     gene            568..1932
                     /locus_tag="P5243_07915"
     CDS             568..1932
                     /locus_tag="P5243_07915"
                     /inference="COORDINATES: protein motif:HMM:NF025654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HRS19415.1"
                     /translation="MTLIILNILFAITSIVLCCCAPTSYNYHFCLAILILYIVQNVLF
                     FYFNKRRNWLGFEFFFAISFAFTNFIYPVFYFPTRPDYLFFSIPFNVNVISRATAIAY
                     FAYAFYMLGATKLLKMKREEPVSPTFKFGINQFLWLFVITVVAFACYIGLGGWGALQD
                     VYSGTANIRDTGIYSYFNNIFTIACYLMAIFVFRIKKQKQWFYLLFICVCMFFIISTG
                     SRSLALGLALILIVGFNNNVRRFSILEIFSLIFIGAFLLFLVMRIRHSQINSETITAT
                     IQNIRIENIMDIFGDLIVNNRNLYVLVDYADTHELTFFHGMLIDIFSPIPRASSWLVK
                     IFNEPIELLHGGDLPTYLYLGSGSVWGLGTNMVGEAFRSFGYVGTAISMMLIGQIIKS
                     SYYYSYNNCYAYAIYYLFVSHAVFYPRAPLLFDPRTLVWGMLLIFVVYSITPKKSIQD
                     EKNK"
BASE COUNT          547 a          350 c          363 g          750 t
ORIGIN      
        1 tgtagctttt tcaaactctc aatggataga cgaatatgga cattctatta aaaggcgtac
       61 gactaaaaca tggtcacgag attttacaat gaacggtatt gattttgtca agaaatacct
      121 gttgggatat aattatattt gcaatgccag tgctgttttg ttcaaacgta cagcccttac
      181 tgccgttaca aacgactaca cgcaatataa agcaagtggc gaccgacttt tttggataca
      241 aatttgtttg caaggaaaag tttgttatgt agccgaaaag ttgaattttt ttcgtcaaca
      301 cacccataaa gtctcgggac acgcagccga aaccggtgtc aatatggtag aagattttag
      361 catctaccaa aaaataaaaa cgcttgttcc gttcaatttt tgggaacaaa gccttatttg
      421 tggctatcat tatcactcca ttcattattg ccagcaattt gttggcaatg ggaaacaatt
      481 tgcattgtct gcttgggagc aaacacgttt tttcaatatg ttttcttata taacatacct
      541 ctttttcaga gccatagaga tagtacgatg actttaatta tcttaaatat tttgtttgca
      601 attacctcaa tagttttatg ctgttgtgca ccgacttctt ataactatca cttttgcctt
      661 gcaattctca ttctgtatat agtgcaaaat gttttatttt tttattttaa caaacgtcgt
      721 aattggttgg gatttgaatt cttttttgca atttcatttg cttttacaaa ttttatatat
      781 ccggttttct attttccaac acgtcccgat tacttgttct tttccatacc gttcaatgtt
      841 aatgtaatca gccgtgctac cgctatcgcc tattttgctt atgcttttta tatgttgggg
      901 gcaactaaat tgcttaaaat gaaacgtgaa gagcccgtta gccctacttt taaattcgga
      961 ataaatcaat ttttatggtt gtttgtcatc actgttgtag cctttgcttg ttatataggt
     1021 ttgggtggct ggggtgcttt acaagatgtt tattccggca ctgcaaatat tcgtgacaca
     1081 ggtatttact cttattttaa caacattttt actattgctt gttacctgat ggcgatattt
     1141 gtatttcgga taaaaaaaca aaagcaatgg ttttacctgc tgtttatatg cgtatgtatg
     1201 ttttttatta ttagtaccgg ctcgcgttct ttagcattag ggcttgctct tattctcatt
     1261 gttggtttta ataataatgt tcgtcgtttt tcgattttag aaattttttc tttgattttt
     1321 attggtgcat ttttgctgtt tttagtcatg cgcatacgac actcgcaaat caatagcgaa
     1381 acaattacag caaccataca aaatattcgt attgaaaata taatggatat ttttggcgat
     1441 ttaatcgtta ataatcgaaa tttgtatgta ctagtagatt atgcagatac acatgaacta
     1501 actttttttc atggcatgtt gattgatatt ttttcgccca ttccaagagc atcttcatgg
     1561 ttggtcaaaa tattcaacga acctattgag ttgctgcatg gtggagattt gccaacctat
     1621 ctgtatttag gcagtggctc tgtgtggggt ttgggtacga atatggttgg cgaagcattc
     1681 cgctcctttg gttatgtggg cactgccatt tctatgatgt taatcgggca aattataaaa
     1741 tcaagttatt attattcata taataattgt tatgcctacg ccatttatta tctatttgtc
     1801 tctcatgctg tgttttatcc ccgagctccg cttttgttcg acccgcgcac attagtttgg
     1861 ggaatgttac tgatattcgt tgtttactca attactccaa aaaagtcaat tcaagacgaa
     1921 aaaaataaat gaaactgctc tattgcatac ctgctttgta caataccgga ggcatggaac
     1981 gcgtgctgac cgagaaagtg aactttctcg
//
LOCUS       DAOTOW010000439         2006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43786_length_2006_cov_3.855459, whole genome shotgun sequence.
ACCESSION   DAOTOW010000439 DAOTOW010000000
VERSION     DAOTOW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43786_length_2006_cov_3.855459"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="P5243_07920"
     CDS             complement(<1..1675)
                     /locus_tag="P5243_07920"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRS19416.1"
                     /translation="MKVLKFGGTSVKNAENISKVIEIVRGDIYQHEVLVVVSAFGGIT
                     NLLIETAQKAAKGDESYKALFNKISDAHAQVIEQLLSGDLRTQCESYIVEQLSNFKDI
                     LHGLYLLRELSTRSLDLVSSFGERLSAYIIAHAFTAKGIPARYLDAREVIKTDKTFGA
                     AKVNFEKTNTLIAQKVKNSNTLWIATGFIASTDDDETITLGRGGSDYSGAIFARALEA
                     SILEIWTDVDGMMTADPRKVKKAYPISNITYSEAMELSHFGAKVLEPRTVIPAMAIGI
                     PVLIKNTFNPTAPGTLITAQNEQSAHRVQGISSIDDISLITVQGSGMIGEAGISMRLF
                     RAIAKESINVILITQGSSEHSITFAVLPKDALRSVELLKQEFELEIKTNLIDDIKAEH
                     NLAIVSAVGENMKNIPGVSGKFFNALGINGINVHAIAQGASELNISAVINGNDTHKAL
                     NALHESFFLSNTKTINVFVVGTGVIGGELLKQIEAQHSTLVLQNNIDVKVVGISNIDG
                     YIIDEEGIDLQNWEHDLKNAGKKPNIDAFVNEMKALNLRNSVFVDNTASY"
     gene            complement(1761..>2006)
                     /locus_tag="P5243_07925"
     CDS             complement(1761..>2006)
                     /locus_tag="P5243_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19417.1"
                     /translation="HEIALLQKKIRALQDELFVSEKQSIEQELALYIMQRMYFERGKI
                     QYQTLHDEYINQAKQMLLNPKEYNNILQGISGVHKKK"
BASE COUNT          604 a          395 c          278 g          729 t
ORIGIN      
        1 cataacttgc agtattatca acaaaaacac tgtttcttaa attaagagct ttcatttcat
       61 ttacaaatgc atctatattc ggttttttgc cggcattttt taaatcatgc tcccaatttt
      121 gcaaatctat tccttcttca tctattatat atccgtctat atttgaaatt cctacaactt
      181 taacatcaat attattttgt aaaactaatg ttgaatgttg agcttctatt tgcttaagca
      241 attcaccgcc aattactcct gtacctacca caaatacatt aatagtttta gtattggaca
      301 agaaaaacga ttcatgcagc gcatttaatg ctttatgagt atcatttcca ttaattacag
      361 ccgaaatatt taactcactt gctccttgcg caatagcatg tacgttgata ccatttatac
      421 caagagcatt gaaaaatttg cctgaaactc ccggaatatt tttcatattt tcacctacag
      481 ccgatactat tgccaaatta tgttctgctt taatatcgtc aatgagattc gttttaattt
      541 ccaactcgaa ttcttgcttg agtaattcta cagaacgcaa agcgtctttg ggtaacacag
      601 caaaggtaat agaatgttcc gatgaacctt gtgtaataag aattacattg atagattctt
      661 ttgctatagc tctaaacaaa cgcattgaaa tacctgcctc tcctatcatt ccactacctt
      721 gtactgtaat aagacttata tcatcaatag aagaaatacc ttgtacccta tgagcagatt
      781 gttcattttg agcagtgatt aatgtacccg gagctgttgg attaaatgta tttttaataa
      841 gtaccggaat tccaatagcc atagctggta ttacagttct aggctcaaga acttttgccc
      901 caaaatgcga taattccatc gcttcggaat aggtaatatt agaaatagga tatgcttttt
      961 ttacttttct tgggtctgcc gtcatcattc cgtcaacatc tgtccaaatt tcgagtatac
     1021 ttgcctccaa agctcgtgca aaaatagctc cggaataatc tgaaccacct ctgccaagag
     1081 ttattgtttc atcatcatcg gtacttgcta taaaccctgt tgctatccac aatgtatttg
     1141 aattctttac tttttgagct attaatgtat ttgttttttc aaaatttacc tttgcggccc
     1201 caaaagtttt atcggttttt ataacttctc tagcatctaa ataccgtgca ggaatacctt
     1261 tggctgtaaa tgcatgagct ataatatatg ccgacaatcg ctccccaaaa ctcgaaacca
     1321 agtcgagtga acgagtagac aattctcgca ataaatataa tccgtgtaaa atatctttga
     1381 aattagataa ttgttctact atatatgact cacattgcgt tctcaaatca ccggaaagta
     1441 attgttctat tacctgtgca tgtgcatcac ttattttatt aaacaatgct ttgtatgact
     1501 catcaccttt tgctgctttt tgtgccgttt ctataagcaa attagttatg ccgccaaatg
     1561 ccgacactac caccaatact tcgtgttgat aaatatcacc gcgaacaatt tctattactt
     1621 tagaaatatt ttcggcattt ttaaccgatg ttccgccaaa ttttaaaact ttcatagcat
     1681 gaatttttgt actgtgcgta aaaataatat ttgtactacg ttttacaaaa aaaatacagt
     1741 ttatttttac aacttattat ttacttcttt ttgtgaactc ccgaaatgcc ttgcaaaata
     1801 ttattatatt ctttaggatt cagcaacatt tgctttgctt gatttatata ttcgtcatgc
     1861 aaagtttgat actgaatttt acctcgttca aaatacattc gttgcataat gtatagagct
     1921 aattcttgtt ctatactttg tttttcgctc acaaataact catcttgcag tgcacgtatt
     1981 ttcttttgca ataatgcaat ttcatg
//
LOCUS       DAOTOW010000440         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43852_length_2004_cov_3.621344, whole genome shotgun sequence.
ACCESSION   DAOTOW010000440 DAOTOW010000000
VERSION     DAOTOW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43852_length_2004_cov_3.621344"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5243_07930"
     CDS             <1..478
                     /locus_tag="P5243_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRS19418.1"
                     /translation="QQMQGNEYVDTIHYDRLHIQTLNSNLKRISFFLLGLGLLLFGIS
                     IVLIHNTIRLSVYSKRFIINTMQLVGATNAYIRQPFILRGAFQGFVSALIAIALLSGF
                     LFFLQKEIKDFIKYLDFELLGILFLSIILIGIALSTIATYFAVNRFLNIHKDDLYI"
     gene            485..787
                     /locus_tag="P5243_07935"
     CDS             485..787
                     /locus_tag="P5243_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRS19419.1"
                     /translation="MVTEKGQSNDTQFALGKENYILMAVGVVILIVGFILLSGGKAVN
                     PNDFYPNGDPSQTPEIFSFRRITLAPIVILFGFFFEIFAIMVNPDSKIIKLIFRTK"
     gene            784..1590
                     /locus_tag="P5243_07940"
     CDS             784..1590
                     /locus_tag="P5243_07940"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRS19420.1"
                     /translation="MSWIQALIIAIVEGITEFLPISSTGHMIITQHLLGVTDSDFVKI
                     FIVNIQFGAILSVLVLYWKRFVKSIEFYKLLLVAFLPAAIVGFVFGDFIDSLLENIWV
                     VASSLVLGGIVLLFVDRWFIKNENQLQEAENPTYKKSFFIGCFQIIAMIPGVSRSAAT
                     IIGGLVQGMSRKKAAEFSFFLAVPTMAAASGYKLLKSFLETPQVITDSANISILLFGN
                     VIAFIVAMLAIKFFISFLTKYGFKLLGYYRIIVGGVIITMLCLGINISVV"
     gene            1593..>2004
                     /locus_tag="P5243_07945"
     CDS             1593..>2004
                     /locus_tag="P5243_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HRS19421.1"
                     /translation="MIIDRSVHSLQGIDITQGHVLLIHKDKHWTSFDVVNKVRYMIKK
                     TYNIKKIKVGHAGTLDPLATGVLVIGIGKETKNLESYQNQVKEYKAEITFGATTPSYD
                     LETAPQGNFPIDHITKEQIEETLQQKFNGTIKQTP"
BASE COUNT          684 a          259 c          347 g          714 t
ORIGIN      
        1 acaacaaatg cagggtaatg aatatgttga tactattcat tatgatagat tacatattca
       61 aactttaaat tcaaatctta agcgtattag ttttttcttg ttgggattag gattgttgtt
      121 attcggaata tcaatagttt taatacataa cactattcgt ctttcggtgt attccaaacg
      181 atttattata aacactatgc agttagtggg agcaaccaat gcatatattc gtcaaccttt
      241 tatattgcgc ggagcgtttc aaggttttgt tagtgctctt attgctatag cattgttgag
      301 tggctttttg ttttttttac aaaaagaaat aaaagatttt atcaaatacc ttgactttga
      361 gctattgggt atattatttt tgtccatcat tttaataggc attgccttat cgacaattgc
      421 cacatatttt gcggtaaata gatttttaaa tattcacaaa gacgatttat atatataaaa
      481 aagtatggtt acagaaaaag gtcaatcaaa cgacactcaa tttgcgttag gtaaagagaa
      541 ttatatttta atggcggtag gagtggtaat tttaattgta ggatttatat tactttctgg
      601 gggtaaggca gttaatccta atgattttta tccaaatgga gacccatcac aaactcctga
      661 aatttttagt tttagaagaa taacattggc tccaatagtt atattattcg gatttttctt
      721 cgaaatattt gctataatgg ttaatcctga ttcaaaaatc ataaaactca tctttcgtac
      781 aaaatgagtt ggattcaagc acttataata gcaattgtag aaggcattac agagttttta
      841 ccaatttctt ctacaggaca tatgattata acacagcatt tgttaggtgt aacagatagt
      901 gattttgtaa aaatttttat tgtaaatatt cagtttggag caatactgtc tgttttggta
      961 ttgtattgga aacgatttgt aaaaagtata gaattttata aacttttatt agtagcattt
     1021 ttacctgctg ctattgttgg atttgtattt ggtgatttta tagattcact cttagaaaat
     1081 atttgggtag tagcaagttc attagttttg ggaggaatag tattattgtt tgtagataga
     1141 tggtttatca agaatgaaaa tcagttgcaa gaagctgaaa atcctacgta taagaaatct
     1201 ttttttatcg gctgttttca aattatagca atgattcccg gcgtttctcg aagtgctgca
     1261 actatcattg gagggttagt acaaggaatg tcaagaaaaa aggctgcaga attttcattt
     1321 tttcttgcag ttccaacaat ggccgcagct tcaggttata aattgttgaa atcgtttctt
     1381 gaaacaccac aggttattac agattcagca aatatttcaa ttctattatt tggaaatgta
     1441 atagcattta ttgtagctat gcttgcaatt aaatttttta tttcgtttct cacaaaatat
     1501 ggcttcaaac tattgggtta ttatagaata attgtaggtg gtgttataat aactatgctt
     1561 tgtttaggaa ttaatatatc agtagtataa ttgtgattat tgatagaagt gtacattcat
     1621 tacaaggaat tgatattacg caaggacatg tgttattaat tcataaagat aaacactgga
     1681 catcatttga tgtagttaat aaagtacgat acatgattaa aaaaacgtac aatataaaga
     1741 aaattaaagt aggtcatgcc gggactcttg atccattggc tacaggagtg ttggtaatag
     1801 gtataggtaa agaaactaaa aacttggaat catatcaaaa tcaagtaaaa gaatataagg
     1861 cagaaattac ctttggtgca acaactccat catatgattt agaaactgca ccacaaggta
     1921 attttcctat agaccatata acaaaagaac agatagaaga aacgttacaa caaaaattta
     1981 acggtacaat taaacaaacc ccac
//
LOCUS       DAOTOW010000441         2003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_43882_length_2003_cov_3.142197, whole genome shotgun sequence.
ACCESSION   DAOTOW010000441 DAOTOW010000000
VERSION     DAOTOW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43882_length_2003_cov_3.142197"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..>2003
                     /locus_tag="P5243_07950"
     CDS             69..>2003
                     /locus_tag="P5243_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS19422.1"
                     /translation="MHFNDNIDALNTIRFINGTNRSIFLTGKAGSGKTTLLKNIVSNT
                     HKQTVIVAPTGIAALNAGGVTIHSLFQLPFGAFVPAAHLDGIQTISFEVHTLAQLPKE
                     IVKLSSAKRNLIRHMELLIIDEVSMLRADLLDAIDTVLRHIRRRKHDAFGGVQVLFIG
                     DMLQLPPIVKDDEWSYLQKYYTSLYFFDALVFAQEKPIVIELQKIYRQTDTVFVTILN
                     NLRNNIFTQADLQILNKRYIPNFIQKSTDSYIFITTHNRKANEINARELEKLPTDIFT
                     YTAKIQHDFPEHLYPVDTTLELKVGAQVMFIKNDYSGGSQYFNGKIGRISYLEQDGIE
                     VEFPDTGNTVKVDLYTWENKRFVLNYETGAIDETVIGTFTHFPIKLAWAVTVHKSQGL
                     TFKQAIIDVSEAFAPGQIYVALSRFESLDGLVLSQPIVDEAPEQNERLLSFTNAKHSH
                     ADLEQEYEKASREYLYSSILQTFDFNSLVYELQLHIDTYTKDENRSVKQQFLVQIQDV
                     KHDVVPIVDVSNTFSIQLRNLIQSNTEIHVIKDRIEKAYAYFEPLIGAISKKIFAIME
                     QLQDLRGVKAYTNELSQLELQYYTKLQKIKKCIAFIDAMMHNETIKKDAIVLLQNPQE
                     RYVLQKKTQIIKKVQRKKEKK"
BASE COUNT          724 a          284 c          328 g          667 t
ORIGIN      
        1 aatgttaaaa tgcaaacaat gtaaactttt caatatattt gcaatgcacc ctacaattat
       61 atttttttat gcattttaac gataatattg atgctcttaa cacgattcga tttataaatg
      121 gtacaaatcg atctatattt ttaacaggca aagcaggatc gggtaaaaca actcttctta
      181 aaaatattgt aagcaatact cataaacaaa cggttatagt tgctccaaca ggaatagcgg
      241 ctctcaatgc cggtggtgta actattcatt ctttgtttca gttaccattt ggagcttttg
      301 ttcctgctgc tcacttagat ggaatacaaa caatttcgtt tgaggtgcat actttagctc
      361 aattaccaaa agaaattgta aaattaagtt cggctaaacg taatcttatt cgtcatatgg
      421 aacttttgat aattgatgag gtaagtatgt tgcgtgctga tttacttgat gctatagata
      481 ctgtattacg acatattcgt cgtagaaagc atgatgcgtt tggtggggta caagttcttt
      541 ttattggtga tatgctgcaa cttcctccaa tagtaaaaga tgatgaatgg agctatttac
      601 aaaagtatta tacaagtctt tatttttttg atgcattagt ttttgcacaa gagaaaccta
      661 ttgtaattga gttacaaaaa atatacaggc aaacagacac tgtgtttgta actattctta
      721 ataatttgag aaataatatt tttacacagg ctgatttaca aatacttaat aaacgatata
      781 ttcctaattt tatacaaaaa tcaactgact catatatatt tattactaca cataatcgta
      841 aagctaatga aattaatgct cgagaattgg aaaaactacc aactgatata tttacatata
      901 ccgctaaaat tcaacatgat ttccccgaac atttatatcc ggttgatact acattagaac
      961 taaaggttgg agcccaagtt atgtttatta aaaatgatta ttctggtggt tcacaatatt
     1021 ttaatggtaa aattggtaga atttcttatt tagaacaaga tggaattgaa gttgaattcc
     1081 ctgataccgg aaataccgta aaggttgatt tatatacgtg ggaaaataaa cgatttgttc
     1141 ttaattatga gaccggagct attgatgaaa cggtaattgg aacgtttact cattttccta
     1201 ttaaattggc ttgggctgtt acagttcaca aaagtcaagg cttaacattt aaacaagcaa
     1261 tcattgatgt atcagaggct tttgctcccg gtcaaatata tgtggctttg tctcgattcg
     1321 aatcattaga cggacttgta ttgtcgcagc caattgtaga tgaagctcct gagcaaaatg
     1381 aacgattgct tagttttaca aatgcaaaac attcacatgc tgatttagaa caagaatatg
     1441 aaaaggcatc tcgagaatat ttgtattctt caattttgca aacatttgat tttaattcac
     1501 ttgtgtatga attgcaattg catattgata catatactaa agatgagaat cgttctgtaa
     1561 aacaacaatt tttagttcaa atacaagatg ttaaacatga tgttgtacct attgttgatg
     1621 ttagtaatac atttagtatt caattgcgta atttaattca atccaatacc gaaattcatg
     1681 ttataaaaga tagaattgaa aaagcatatg cctattttga accacttata ggggcaattt
     1741 caaaaaaaat atttgctata atggagcaat tgcaagattt gcgaggagtg aaagcatata
     1801 caaatgaact ttcacagtta gaattgcaat attatacaaa gttgcaaaaa attaaaaaat
     1861 gtattgcatt tattgatgct atgatgcata acgaaacaat aaaaaaagat gcgattgttt
     1921 tgttacagaa tcctcaagaa agatatgttt tacagaaaaa aactcaaata ataaagaaag
     1981 tacagcgaaa aaaagaaaaa aaa
//
LOCUS       DAOTOW010000442         1995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44142_length_1995_cov_2.413402, whole genome shotgun sequence.
ACCESSION   DAOTOW010000442 DAOTOW010000000
VERSION     DAOTOW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44142_length_1995_cov_2.413402"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..153
                     /locus_tag="P5243_07955"
     tRNA            69..153
                     /locus_tag="P5243_07955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Leu,seq:taa)
     gene            complement(348..1925)
                     /locus_tag="P5243_07960"
     CDS             complement(348..1925)
                     /locus_tag="P5243_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRS19423.1"
                     /translation="MNIFRNLLVVSITCIAMNIQLQAQDKNFYIYICFGQSNMEGQGN
                     IETKDRTVDNRFKVFQALDCSNLARTKATWYKALPPTCQCYSRLSPADYFGRTMVANL
                     PDSISIGIINVSVGGCDIRLFDKDIYTDYDSTYTESWFTSKVLSYNGNPYQYLIDLAK
                     LAQQDGVIKGILLHQGETNTGNNQWPSYVQKIYNDMIADLSLDATEVPLLAGEVLQTS
                     GSCCSSMNTIINKLPQTLPNSYVISSAGCPGQDNAHFNSEGYRILGRRYAVQMLSLMG
                     YTTHYAEVECGTVGNDWYILSDKNASNTRYVTAKPGLESKNSAPTQADAIIPMSFSID
                     KDTTYYIYGRFNNPSNTSDAFWLQINDEPFILCDDLTTSGWEWKELTHVDLQAGTHTI
                     KIAYAEQGASIDKIAIKNAQIPPSSVGEEANNICDAPIINNLNSVDDLHNNLGQNFPN
                     PVIKNTTIPFSVVAETHVTIKLYNAQGVEVSEILSKTCNAGNHTVEWKRKNIPSGLYY
                     YTITTNDFTSTRSLLIK"
BASE COUNT          616 a          328 c          348 g          703 t
ORIGIN      
        1 agttggtaga gcacgacctt gccaaggtcg gggtcgcggg ttcgagtccc gtttctcgct
       61 ccatactcgc cttggtggtg gaattggtag acacgcagga cttaaaatcc tgtggacatt
      121 gcgtccgtgc gggttcaatt cccgcccgag gtacaaaaac ctcagcagtt tcataaattg
      181 ctgaggtttt ttgttttaaa tattattcaa atacttatgg tgttcgttta aaatttgaaa
      241 gaaatagaag tgaatttttg taaaaaacat aataaaaccg cttagaacaa gtatttgaat
      301 tatacaaatt ctaagcggtt tgaactcaaa attacgtcga attgacgtta ttttataagt
      361 aaacttcgag tcgaagtaaa atcatttgta gttatagtat agtaatataa tcccgaagga
      421 atattttttc gtttccattc cacagtatgg ttacctgcgt tgcaagtttt agaaagaatt
      481 tctgaaactt ctactccttg tgcattgtat agttttatgg ttacgtgtgt ttcggcaaca
      541 actgaaaatg gaatagttgt gttttttata accggattag gaaaattttg acctaaatta
      601 ttgtgtaaat catcaacact atttaggttg tttataatag gagcatcaca tatgttattt
      661 gcctcttcgc ctactgacga tgggggtatt tgagcatttt taattgcaat tttatcaatg
      721 cttgcacctt gttcagcata tgctattttt atagtgtgag tgccggcttg taaatctacg
      781 tgtgttaatt ctttccattc ccagccactt gtagttaaat catcacacaa tataaatggt
      841 tcatcattta tttgtaacca aaaagcatca ctggtatttg atggattatt gaatcttccg
      901 taaatataat atgttgtgtc tttgtcaata gaaaatgaca taggtattat agcgtcggct
      961 tgagtaggag cagagttttt actttctaag cccggttttg cggtaacata ccgggtattt
     1021 gatgcatttt tgtctgaaag tatataccaa tcattaccta cagtgccgca ttctacttcg
     1081 gcatagtgtg ttgtatatcc cataagagaa agcatttgta cagcatatct gcgacctaaa
     1141 attctatatc cttcagaatt aaagtgagca ttatcttgtc ccggacaacc tgccgatgaa
     1201 attacgtaag aattaggaag tgtttgtgga agtttattaa taatagtatt catacttgaa
     1261 caacaacttc ccgaagtttg taaaacttca cctgccagca aaggtacttc tgttgcatct
     1321 aatgataaat cagcaatcat atcgttatat attttttgta catacgatgg ccattgattg
     1381 ttgcctgtat ttgtttcgcc ttgatgcaat aaaatacctt ttattacgcc atcttgttga
     1441 gcaagtttag ctaaatcaat taaatattga tatggattac cattgtatga taaaactttt
     1501 gaagtaaacc atgattcagt atatgtagag tcgtaatctg tataaatatc tttatcaaac
     1561 aatcgaatat cacaaccacc taccgatacg tttataattc ctatagaaat actatcaggt
     1621 aaatttgcta ccatagttct gccaaaatag tcggcaggcg ataatcttga ataacattga
     1681 catgtaggtg gcaatgcttt gtaccaagta gcttttgttc ttgctaaatt tgaacaatct
     1741 agtgcttgaa acactttgaa acgattgtca acagttctgt cttttgtttc aatatttcct
     1801 tgaccttcca tatttgattg gccaaaacaa atataaatgt aaaagttttt gtcttgtgct
     1861 tgtaattgta tgttcattgc aatgcacgta atacttacca ctaataaatt tcgaaatata
     1921 ttcatacgag tcatttttta agatattttt ataaaaacaa tattgttaca aaattaaaaa
     1981 aaaaatacat aggaa
//
LOCUS       DAOTOW010000443         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44382_length_1987_cov_2.535714, whole genome shotgun sequence.
ACCESSION   DAOTOW010000443 DAOTOW010000000
VERSION     DAOTOW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44382_length_1987_cov_2.535714"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1294)
                     /locus_tag="P5243_07965"
     CDS             complement(<1..>1294)
                     /locus_tag="P5243_07965"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19424.1"
                     /translation="MTSKNYPTTNWPYGEMYYNVRYFYAANEFTPQQTMRGKQALYGY
                     LHAISPAVQIGCATPNITGVNSICGVTSAVLQSGLTAGGKTFTWYKDGNVISGQTSPS
                     LTITQAGTYRVSVDSNGCVKTSDKIVSGTLQLELGENKNLCTQTEFVLDAQNASVPNV
                     SYVWNTGATTQTITVAKAGTYSVSVSALNCPTVQDNITITSSLLNVTGDTICSPGQVT
                     LTVTGTSTYAWYNVATGGTALSTAKTYSPTISTNTTYYVQETGGFSGSLGKATQGTGD
                     KWSMGSGFTDSDKINKITVLQSLTLESIAVYVVSAGTNVTINIKQNGTVIHTKTIIGL
                     ATGKQTLPLNFFLAPGNYTIDCDGTSGAIYFEASGASFPYTYTGYISFTYNESWQSAW
                     YGFFYDWKILVGSACARTPVKAVIDPTNSKCVSTYTQSI"
     assembly_gap    1295..1304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1305..>1987)
                     /locus_tag="P5243_07970"
     CDS             complement(<1305..>1987)
                     /locus_tag="P5243_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS19425.1"
                     /translation="YRDSAINAYNYASGLSDQMLTNTVDKFTGKDFKATAAAFLYNIT
                     GNTAYETMLNSLSSCTSTTSVIHNSNQNQLYAVAGYLFTKQPRNYPTLYNNMKASIIN
                     EAKTVEANYSTTRPSRRASDNNQGWFVTEMVIQRTIIAHAIADPGADKTLFENALILE
                     ADWSLGRNPLNMIIMTTATTPLASKKSVPNAYTSGWADGTQGVHPGHTPYMNIYDWSP
                     GGMAMGNPS"
BASE COUNT          575 a          387 c          350 g          665 t
ORIGIN      
        1 aatactttgt gtataggtac taacacattt actatttgta ggatcaatta ctgcttttac
       61 gggtgtgcga gcacaagctg aacctactaa aattttccaa tcatagaaaa atccatacca
      121 tgcactttgc caactttcat tataggtaaa tgaaatatag cctgtatatg tatatgggaa
      181 tgatgcacct gatgcttcaa aataaattgc acctgatgtt ccgtcacaat ctattgtata
      241 gtttccgggt gccaaaaaga agttaagagg taatgtttgt tttcctgtag caagacctat
      301 aatagtttta gtatgaataa ccgtaccgtt ttgttttata tttatagtaa cattagtacc
      361 tgcagaaact acatatacag caatactttc taaggtaaga ctttgtaaca cggtaatctt
      421 attaatttta tcgctatcgg taaatccgct acccatactc catttatcac cagtaccttg
      481 tgttgctttt ccgagcgacc cgctaaatcc acctgtttct tgaacataat atgttgtatt
      541 agtacttata gttgggctgt aagttttcgc cgtacttaat gctgttcctc cggttgctac
      601 attataccat gcataggtag aagttcccgt aacggtaaga gtaacttgtc ccggactaca
      661 aatagtatcg ccggtaacat tgagtaaact tgaggtaata gtaatattat cttgaacagt
      721 tgggcagtta agagcactca cgcttaccga atacgtacct gcttttgcca cagtaatagt
      781 ttgtgttgta gctcctgtat tccatacata cgatacattt ggcacacttg cattctgagc
      841 atcaagaaca aattctgttt gtgtacataa atttttattt tcacctaatt caagttgtaa
      901 agttccacta acaattttat cacttgtttt aacacaacca ttactatcta ccgatacccg
      961 atatgttcct gcttgcgtaa ttgtgagcga tggtgatgtt tgcccggaaa tcacgttgcc
     1021 atctttatac cacgtaaatg ttttgcctcc ggcagttaat ccactttgca acacagcaga
     1081 tgttactcca caaatagaat taacaccggt aatatttgga gtagcacatc caatttgaac
     1141 tgcaggactt atagcatgca aatatccata cagagcttgt ttaccacgca tagtttgttg
     1201 aggggtaaat tcatttgctg cataaaagta acgaacatta taatacattt ctccataagg
     1261 ccagttagta gtaggataat ttttactagt catcnnnnnn nnnncgaagg atttcccata
     1321 gccattcccc cagggctcca atcgtaaata ttcatatacg gcgtatgtcc cggatgaact
     1381 ccctgagtac catcggccca tccgctggta tatgcattag gtaccgattt tttagacgca
     1441 agaggagttg tagctgtagt cattataatc atattcaaag gatttcgtcc gagcgaccaa
     1501 tctgcttcta aaataagagc attttcaaat aaggttttat cggctccggg gtctgcaata
     1561 gcatgtgcta taatagttcg ttgtattacc atttctgtta caaaccatcc ctgattatta
     1621 tcacttgctc tccttgatgg acgcgttgta gaataatttg cttcaactgt tttagcttca
     1681 tttataatag aagctttcat attgttgtat agtgtaggat aatttcgtgg ttgcttggta
     1741 aataaatacc cggctacggc atacaattga ttttgattgc tattatgaat taccgatgta
     1801 gtgcttgtgc atgaacttaa actattcaac atagtttcgt aagcagtatt tccggtaata
     1861 ttatacaaaa acgctgcggc agttgcttta aaatctttgc ctgtaaattt atctactgta
     1921 ttggtaagca tttgatccga taatccactt gcataattat aagcattaat agcactgtcg
     1981 cgatatt
//
LOCUS       DAOTOW010000444         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44415_length_1986_cov_2.708959, whole genome shotgun sequence.
ACCESSION   DAOTOW010000444 DAOTOW010000000
VERSION     DAOTOW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44415_length_1986_cov_2.708959"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="P5243_07975"
     CDS             <1..1063
                     /locus_tag="P5243_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19426.1"
                     /translation="AMLSQSSEQSLFVEDEWFITPQLICNASIRIVPYVLLGPSSYYV
                     YNDKGEVTDTVFYTQNQIMYTTIGFEPRLQLQYRFSSDASLKFSSMRTTQQMHMVSML
                     SAALPADIWLPASNFNKPMNGWISSVGYFRNFNDNEFSSSLSLYYKSMQNIIEFKDGF
                     ITLYASSYNQKITHGKGFAFGSEFVIKKIKGDFQGAFSYNFSRSLRCFQDLNQGYLYP
                     ASYDKPHDVSIQLQYKISSQLTCNALWVYNSGKVYSEPVSKYFIGKNVISEYGPINSS
                     RMPAYHRLDIGAQYVIAKTDSYELLSNISIYNVYNRKNTYYIYYETIGNVNEFSIQFV
                     KNFVGLFPILPSVSIQLYF"
     gene            1067..1930
                     /locus_tag="P5243_07980"
     CDS             1067..1930
                     /locus_tag="P5243_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19427.1"
                     /translation="MMKKNVYIINIIIIAISFCSVSCSDNFTHSDFKAGRVVVDGSIE
                     AGSFAKVVLTYSLPLDAKIDSSVYYDLVNTRAKVTIFDGEQSEVLVLIKRKNFFPPHY
                     YTTRTIKGVPGKTYWLEVIYYDDTIRAQTTIPNNVELSKIWSEPHVNDTTKRYLWIQM
                     CDDVQRKDYYRIFTQIKGKQKDFVPTYQSAFSDEYFNGTCPKIMLYKGLENFFDKKRE
                     DTFSVGDTVFVKISSIDKQSYLFWSNYEKEVFNSGNPFAANGSNLTSNVDSGIGIWCG
                     YASKVYSIIIK"
BASE COUNT          687 a          249 c          330 g          720 t
ORIGIN      
        1 tgcaatgctt tctcaaagtt cggaacaatc actgtttgtt gaagatgaat ggtttattac
       61 tccacaactt atttgtaatg cttctattcg tatagttccg tatgttttgc ttggaccttc
      121 ttcatattat gtatataatg ataaaggaga ggtaacagat acagtgtttt atactcaaaa
      181 tcaaattatg tatactacca taggatttga acctcggtta caattacaat ataggttttc
      241 atcagatgca tcgttaaaat tttctagtat gcgtacaacc caacaaatgc atatggtttc
      301 aatgctttct gcagctttgc cggccgatat ttggttgcct gcaagtaatt ttaataaacc
      361 aatgaatgga tggatttcga gtgtggggta ttttagaaac tttaacgata acgagttttc
      421 aagttctctt tctttatatt ataaatcaat gcaaaatatt attgaattta aagatggatt
      481 tataacatta tacgcaagtt cttataatca aaaaattact catggtaaag gattcgcatt
      541 tgggagtgaa tttgtaatta aaaaaataaa aggtgatttt cagggggcat tttcatataa
      601 tttttctcgt tctttacgtt gttttcaaga cttaaatcaa ggatatttgt atccggcgtc
      661 atatgacaaa cctcatgatg tttctataca attacaatat aaaatatcat ctcaattaac
      721 gtgtaatgca ttgtgggtat ataatagtgg aaaagtatat tcagaacctg tgtcaaaata
      781 ttttattggt aaaaatgtaa taagcgaata tgggccaata aatagttctc gaatgcctgc
      841 ttatcataga ttagatatag gagctcagta tgtgattgct aaaactgatt cgtatgaatt
      901 attaagcaat atatcaatct ataatgtgta taatagaaaa aatacatatt atatttatta
      961 tgaaactatt ggaaatgtaa atgagttttc aattcaattt gtaaagaatt ttgtgggatt
     1021 atttcctatt ttaccatctg tttctattca attgtatttt tagtatatga tgaaaaaaaa
     1081 tgtatatatt ataaatatta tcataatagc aataagtttc tgtagtgtat cttgcagtga
     1141 taattttaca cattctgatt ttaaagcagg aagggtggtt gttgatggaa gtattgaagc
     1201 aggatctttc gcaaaagtag tattaaccta ttctttgcct cttgatgcta aaattgattc
     1261 atctgtttat tatgatttag taaacactcg ggcaaaagtt accatatttg atggagaaca
     1321 atcggaggtt ttggtattaa taaaaagaaa aaattttttt ccgcctcatt attatactac
     1381 acgcactata aagggagtac ccggtaaaac gtattggctc gaagttatat attatgatga
     1441 tactatacga gctcaaacaa caattcctaa taacgtagaa ctttccaaga tttggtcaga
     1501 acctcatgta aatgatacaa caaaacggta tttatggatt cagatgtgtg atgatgttca
     1561 acgtaaagat tattatagga tttttactca aattaaagga aaacaaaaag attttgtacc
     1621 aacatatcaa tcggcatttt ctgatgaata ttttaatggt acatgtccta aaattatgct
     1681 ttataaagga ttagaaaatt ttttcgataa aaagcgtgaa gatacatttt ctgttggaga
     1741 tacggtattt gttaaaataa gtagtattga caaacaatcg tatttgtttt ggagtaatta
     1801 tgaaaaagag gttttcaatt caggaaatcc ttttgcagca aatggttcaa atcttacttc
     1861 aaatgttgat agtggaattg gtatatggtg cggatatgca agtaaagtat attcaattat
     1921 aataaaataa ccatacgtgt aagtttttat tctcttttat tactaatata taagttatta
     1981 ataatc
//
LOCUS       DAOTOW010000445         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44546_length_1981_cov_3.160955, whole genome shotgun sequence.
ACCESSION   DAOTOW010000445 DAOTOW010000000
VERSION     DAOTOW010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44546_length_1981_cov_3.160955"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5243_07985"
     CDS             complement(<1..194)
                     /locus_tag="P5243_07985"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRS19428.1"
                     /translation="MAIIRISKEFVFDMAHALHKYDGLCKNIHGHTYKLQVTLRGPIN
                     TDENSTKLGMVLDFSELKRIV"
     gene            complement(327..704)
                     /locus_tag="P5243_07990"
     CDS             complement(327..704)
                     /locus_tag="P5243_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19429.1"
                     /translation="MAEYKVLLVDNNTIRRKLLAGYLTQLQCLYSEAKNGKQAIEMIA
                     NDSFDLVLMDIQLPVIDGIEATKIIRKKLPFPKNRVKIIANTTTNYKDFFNQFYDVGF
                     NDVLPTPSTLDSLKTIIEYHIRD"
     gene            858..>1981
                     /locus_tag="P5243_07995"
     CDS             858..>1981
                     /locus_tag="P5243_07995"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRS19430.1"
                     /translation="MKVLKFGGTSVGSPERMKNVSKLINDGNKQIVVLSAMSGTTNSL
                     VEISDYYYKGNADAAAQVILALEQKYNSVIQELLQKEETRKKAIEIVGNEFNIIRSFA
                     NKQFSLAGEKSILARGEIISTNLVQLYHEEIGVQSVLLNALDFMRVDADGEPILSEIE
                     TRLSKILEQYTQTTLFITQGFICVNPNEEIDNLKRGGSDYSATLIGAAIQSQVVEIWT
                     DIDGVHNNDPRIVKNTYPIAELSFDEAAELAYFGAKILHPSTVLPAKVKNIPVLLKNT
                     MEPQAKGTTISNKSVGKGIKAVAAKDGLTAIKIKSGRMLLAYGFLRKVFEIFENYKTP
                     IDMITTSEVAISLTIDKTDYLAQITEELKALGSVEIDKDQT"
BASE COUNT          719 a          254 c          323 g          685 t
ORIGIN      
        1 acaattctct ttaattcact aaagtcaagt accataccta attttgtgga attttcatct
       61 gtatttattg ggccacgtaa cgttacttgt agtttgtatg tatgtccatg tatgtttttg
      121 cacaaaccat catatttatg aagagcatgt gccatatcaa aaacaaattc tttacttatg
      181 cgtattattg ccatattatt ttttttacat aattactcaa atatttggta ttacccaaat
      241 aaccacaata aaattttttt aagtatttgt agaatatagt atacctaacc ttacgagtaa
      301 ataagaattt cttaagattt aaacaattag tctctgatat gatattctat tatggtttta
      361 agcgaatcta aagtacttgg tgttggtaat acatcattaa agccaacatc ataaaattga
      421 ttgaagaaat ctttgtaatt agttgtggta tttgcaatta ttttaactct atttttggga
      481 aagggtaatt tttttcttat aatttttgta gcttctatac catcaattac aggcagttga
      541 atgtccatta atactaaatc aaaagaatca ttagcaatca tttcaattgc ttgttttccg
      601 ttttttgctt cagaatacaa acattgtaat tgcgtaagat atcctgcaag caattttctg
      661 cgaattgtat tattatcaac tagtaatact ttgtattcag ccataaaaat atctttttgt
      721 aatagagagg tgcgtaaata tataaaaaag tattgagaaa tgcgtatttt tttatgaaat
      781 attttagttg taatgaaaat ttttatttca aatagtataa ttatttttgt tttctgaaac
      841 taaaaaatcg aacaataatg aaagttttaa aatttggagg tacatccgta ggttcacccg
      901 agcggatgaa aaatgtatca aagttaataa atgatggaaa taagcaaata gttgttttat
      961 ctgcaatgtc gggaacaaca aatagtttag ttgaaatttc tgattattat tataagggaa
     1021 atgcagatgc tgcagcgcaa gttattttag cacttgagca aaaatataat tctgtgattc
     1081 aagaattatt acaaaaagaa gaaacacgaa aaaaagcaat tgaaatagtg ggtaacgaat
     1141 ttaatataat tcgttcattt gctaataaac aattttcatt agccggtgaa aaatcaatat
     1201 tagctcgcgg tgaaataatt tctacaaatt tggttcaatt gtatcatgaa gaaataggcg
     1261 tgcaatcagt attgcttaat gctttagatt ttatgcgtgt tgatgcagac ggagaaccta
     1321 tattgtctga aatagaaaca cgactatcaa agattctgga acaatataca caaacaacat
     1381 tatttattac gcaaggtttt atttgtgtaa atccaaatga agaaatagat aatcttaaac
     1441 gtggaggaag tgattattcg gcaacgttaa ttggagctgc tattcaatcg caagtggttg
     1501 aaatttggac agatatagat ggtgtacata ataatgatcc tagaattgta aaaaatacct
     1561 atccaattgc agaattgtcg tttgatgaag ctgcagagtt ggcatatttt ggagctaaaa
     1621 ttttacatcc ttctacagtt ttaccggcta aagttaaaaa tatacctgtt ttgcttaaaa
     1681 atacaatgga accacaagca aaaggaacta ctatttcaaa taagtcggta ggaaaaggaa
     1741 taaaagctgt agctgcaaaa gacggattaa cagcaataaa aataaaatca ggtcgaatgt
     1801 tgttagcgta tggttttttg cgtaaagtgt ttgaaatttt cgagaattat aaaacaccta
     1861 tagatatgat tacaacttcg gaagtagcta tttctttaac tatagataaa acagattatt
     1921 tagcgcaaat tactgaagaa ttaaaggcat taggctctgt tgaaattgat aaagatcaaa
     1981 c
//
LOCUS       DAOTOW010000446         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44575_length_1980_cov_3.945455, whole genome shotgun sequence.
ACCESSION   DAOTOW010000446 DAOTOW010000000
VERSION     DAOTOW010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44575_length_1980_cov_3.945455"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="P5243_08000"
     CDS             <1..373
                     /locus_tag="P5243_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS19431.1"
                     /translation="EKTKYNGGKYINPYTNSSKFEVQMIEPTILHTIKNLQPGQITEP
                     ILTYDETGMQVYKIFKLISITPEHKATLLDDYQTIKEKALQEKKQKAIAQWIANKQET
                     TYISIPNSKYSNCAFKYNWKK"
     gene            383..1324
                     /locus_tag="P5243_08005"
     CDS             383..1324
                     /locus_tag="P5243_08005"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRS19432.1"
                     /translation="MNIAVIGIGGIGGYIGAYLAKHYTKTNEHTITFIQRGVHGETIA
                     NQGLTFIAKQTFITHPHAIYSTPLENNIFDIVFFCVKSKDLESTARDFSNHMHANTVC
                     ISLLNGVNNAKRLETVLPHHRILDGCIYVSAAITAPGVVTQKGGSGLIYFGPTNKKIR
                     DIDTQIFSILQQASIKSELSTDITTEVWRKYLFICPFATMTSLYNLPIGVLIRKPELF
                     NQTQSLMQEIVNLATIHKAHLTTNDIDSAIELAFKIPEQTPTSMQLDIYAGKQPELEV
                     FTQYVVSESKTLGIDCPTHSMLLSLLKDVIKKQVYHG"
     gene            1317..1934
                     /locus_tag="P5243_08010"
     CDS             1317..1934
                     /locus_tag="P5243_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HRS19433.1"
                     /translation="MAKKYYVVWKGHQTGIFTSWNMCKPLIEGYKGAVYKSFDTYEEA
                     KEAFENQQSQTIRNQHLSKKQRIITAYPIQDSLAVDAACSGNPGPMEYRGVYVKTGQE
                     LFKIGPLQDGTNNIGEFLAIVHGLAYLQKIQSNIPIYTDSVTALSWIKNKHCKTTVSE
                     TSRNEKIFELIDRAEHWLTHNTYNNQILKWDTQHWGEIPADFGRK"
BASE COUNT          776 a          323 c          298 g          583 t
ORIGIN      
        1 tgaaaaaact aaatataatg gtggtaaata tataaaccca tatacaaact catcaaaatt
       61 tgaagtgcaa atgatagagc caacaatatt gcatactatt aaaaatttac aacccggaca
      121 aattacagaa cctatactta catatgatga aacaggtatg caggtatata aaatttttaa
      181 attaatatct attactcctg aacataaagc aactctgctt gatgattatc aaactataaa
      241 agaaaaggct ttgcaggaga aaaaacaaaa agcaatagct caatggatag ccaacaaaca
      301 agaaactacg tatatttcaa tacctaattc taaatattct aattgtgcat ttaaatacaa
      361 ttggaaaaaa taaaaataga ctatgaatat agctgtaata ggaattggtg gtataggtgg
      421 atatatagga gcttatttag caaaacacta taccaaaacc aacgaacata ctattacatt
      481 tatacaacga ggtgttcatg gtgaaacaat tgccaatcaa ggtttaacat ttattgcaaa
      541 acaaacgttt attactcatc cacatgctat ttatagtact ccattagaaa ataatatatt
      601 tgacattgtg tttttttgcg taaaaagtaa agatttagaa tcaactgctc gtgattttag
      661 taaccacatg catgcaaata ctgtatgtat aagtttacta aatggtgtaa ataatgcaaa
      721 acgacttgaa acagttttac cacatcatag aatacttgat ggttgtatat acgttagtgc
      781 tgctattact gctccggggg tagtaaccca aaaaggtgga tcggggctta tatattttgg
      841 accaactaat aaaaaaatac gtgacataga cacacagata ttttcaatac ttcaacaagc
      901 atctattaaa agtgaattat ccaccgatat taccaccgaa gtttggagaa aatatttgtt
      961 tatatgtcca tttgcaacaa tgacctcgct ctataattta ccaataggtg ttcttatacg
     1021 taaaccggaa ttgttcaatc aaacacaatc gctaatgcaa gaaattgtaa atcttgcaac
     1081 aatacacaaa gcacatctta ctacaaacga tatagattct gcaatagaat tagcatttaa
     1141 aattcctgaa caaactccta cttcaatgca acttgatata tatgcaggca aacaacccga
     1201 attagaagtg tttacgcaat atgtagtatc cgaatctaaa acacttggta ttgattgccc
     1261 aacacattcc atgcttttat ccttacttaa agatgttata aaaaaacagg tatatcatgg
     1321 ctaaaaaata ttatgttgtt tggaaaggac accaaacagg aatatttaca tcgtggaata
     1381 tgtgcaaacc attgatagaa ggttataaag gagctgttta taaatcattt gatacttatg
     1441 aagaagctaa agaagctttt gaaaatcaac aatcacaaac tatacgcaac caacatttat
     1501 ctaaaaaaca acgaataatt actgcatatc caattcaaga cagcttagcc gtagatgccg
     1561 catgcagtgg taacccggga cctatggaat atagaggtgt atatgttaaa accgggcaag
     1621 aactttttaa aattggacca ttacaagatg ggacaaataa tattggagaa tttttagcaa
     1681 ttgttcatgg cttggcttat ttacaaaaaa ttcaatcaaa catacctatt tatacagatt
     1741 ctgtaactgc tttgagttgg ataaaaaaca aacactgtaa aactacagta agtgaaactt
     1801 ctcgcaacga aaaaattttt gaacttattg acagagcaga acattggctt acacacaata
     1861 cgtataacaa ccaaatttta aaatgggaca ctcaacactg gggtgaaatt cctgcagatt
     1921 ttggaagaaa ataaattatt tcaaaattct ttttgctatt tctatatcat gcttaattgc
//
LOCUS       DAOTOW010000447         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44605_length_1979_cov_3.619543, whole genome shotgun sequence.
ACCESSION   DAOTOW010000447 DAOTOW010000000
VERSION     DAOTOW010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44605_length_1979_cov_3.619543"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="P5243_08015"
     CDS             complement(<1..301)
                     /locus_tag="P5243_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19434.1"
                     /translation="MKKLVFFAIMLLSIVTSVSAQRANQKWYRHAIGIRVENEDLIGI
                     SYDRFLSNQTAGELFLMSDLSSGAELTGLYKYVKSLPDIPASVRWYAGCGVHIGQW"
     gene            complement(309..803)
                     /locus_tag="P5243_08020"
     CDS             complement(309..803)
                     /locus_tag="P5243_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19435.1"
                     /translation="MVIQNNTFTGKSKIIRIISLIFVIIFSSVFLFWDVALYEFGVTY
                     SLSWIQTLFFTLLIFSIYGVFVWQRFRKKYYFVYVTDDDNEHIIFRFYHIRTFGKKFN
                     TYKIPIIKFHSYEITKDSSNTELHLYQKVNEKIAKYPPISLNAYSQQEIHELEQLLKS
                     YLPK"
     gene            958..>1979
                     /gene="ychF"
                     /locus_tag="P5243_08025"
     CDS             958..>1979
                     /gene="ychF"
                     /locus_tag="P5243_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRS19436.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNCLTNAKAQAANFPFCTIEPNIGMI
                     TVPDSRLNELEKIVKPERVVPTTVEIVDIAGLVKGASRGEGLGNKFLANIRETDAIIH
                     VLRCFENENITHVDGSINPVRDKETIDTELQLKDLETIEGRIQKVKKQAQTGGDKEAK
                     YVYDILERVKSHVEAGNSVRSLGLDENEVKAIYDLHLITLKPVMYVCNVDESGIKGNA
                     HVDALKKAVAQENAEVLVLATAIEAEIAELEDPEERQLFLQDLGLEEPGLNKLIRTAY
                     SLLNLETYFTAGVKEVRAWTYHKGFKAPQAAGVIHSDFERGFIKAEVIKFADFIKYGS
                     EQAVKEA"
BASE COUNT          729 a          261 c          340 g          649 t
ORIGIN      
        1 cccactgtcc tatatgaaca ccacagccgg cataccaacg taccgatgca ggaatatcgg
       61 gtaatgattt aacatattta tataaacctg taagttctgc accacttgat aaatcgctca
      121 tcaaaaataa ttctcctgct gtttgattag ataaaaatct atcgtaggaa ataccaatta
      181 agtcttcatt ttcgacacgt attccaatag catgacgata ccatttttga tttgcacgtt
      241 gtgctgaaac tgaagttaca atactaagta acataattgc gaagaatact aattttttca
      301 tataactatt attttggtaa ataacttttt aataattgtt ctaattcatg tatttcttgt
      361 tgtgaatatg catttaaact aataggtgga tattttgcaa ttttttcgtt tactttctgg
      421 tataaatgta attcagtatt tgaagaatct tttgtaattt catatgagtg aaatttaata
      481 ataggtattt tatatgtgtt aaattttttt ccaaatgtgc gaatatgata aaatctgaaa
      541 attatatgtt cattatcgtc atcggtcaca tatacaaaat aatatttttt tcgaaatcgt
      601 tgccaaacaa aaacgccata aatactaaat ataagtaatg tgaaaaataa agtttgtatc
      661 cacgataacg aatatgtaac tccaaattca tataaagcta catcccaaaa taaaaataca
      721 gaactaaaaa taataacgaa tattaaagaa ataattcgta taatttttga tttgcctgta
      781 aaggtattat tttgaattac catgtaatgt taattgttac tgtattatgc aaatatagaa
      841 aaaagttaga ttttaaaaag gtaatgttgt aaaatgtttc gcaattatga tattttaggt
      901 ttaaacattt ttaccacagt ataaattcat tacttttgca aaaaaaagta gatttatatg
      961 ggattaaaat gtggaatagt aggattacca aatgttggaa aatcaacctt atttaattgt
     1021 ttaacaaatg caaaagctca agctgccaat tttccatttt gtactattga accaaatatt
     1081 ggaatgatta ctgttcctga cagtagactc aatgaattag aaaaaattgt aaagcccgaa
     1141 cgtgtagttc ctactaccgt tgaaattgtt gatatagcag gcttagttaa aggtgcaagc
     1201 cgtggcgaag gattaggaaa taagtttttg gcaaatatca gagaaaccga tgctataatt
     1261 catgtgttgc gttgttttga aaatgaaaat ataactcatg tagatggatc tataaatcct
     1321 gtaagagata aagaaactat tgataccgaa ttgcaattaa aggatttaga aactatagag
     1381 gggcgtattc aaaaagtaaa aaaacaagct caaaccggtg gcgataaaga agctaaatat
     1441 gtatatgata tattggaacg agttaagtca catgtagaag ccggaaattc tgtgagaagt
     1501 ttagggttag acgaaaatga agtaaaagct atttatgatt tacatttgat aaccttaaaa
     1561 ccggtaatgt atgtttgtaa tgttgatgaa tcgggaatta aaggtaatgc gcatgttgat
     1621 gcattaaaaa aagcagttgc acaagaaaat gccgaagtac ttgtattggc tacagctatt
     1681 gaagctgaaa ttgcagaact tgaagatccc gaagaacgac aattgttttt acaagattta
     1741 gggttagaag aaccgggttt aaataaatta attcgtaccg catatagttt attgaatctt
     1801 gaaacctatt ttaccgctgg agttaaagaa gtacgcgcat ggacatatca taaaggtttt
     1861 aaagctcctc aggctgccgg tgtaatacat tctgattttg aacgaggatt tataaaagct
     1921 gaggttataa aatttgccga ctttattaaa tatggttcgg aacaagctgt aaaagaagc
//
LOCUS       DAOTOW010000448         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44623_length_1979_cov_2.054054, whole genome shotgun sequence.
ACCESSION   DAOTOW010000448 DAOTOW010000000
VERSION     DAOTOW010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44623_length_1979_cov_2.054054"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="P5243_08030"
     CDS             complement(<1..201)
                     /locus_tag="P5243_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRS19437.1"
                     /translation="MNFVQELTWRDMIHTVMPGTEEQLQKELTSAYLGIDPTADSLHI
                     GHLVGVMMLKHFQAAGHKPIALI"
     gene            531..824
                     /locus_tag="P5243_08035"
     CDS             531..824
                     /locus_tag="P5243_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19438.1"
                     /translation="MCRGKAYGTWFDLPDVTYVSVFQAKMVHTLHSVTYRIFETEEKE
                     YTINFIYDKRKRLQVYSSKDKEYSIELAKHIAHKLGVKLYNATNTHGEWCDEL"
     gene            1044..>1979
                     /locus_tag="P5243_08040"
     CDS             1044..>1979
                     /locus_tag="P5243_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19439.1"
                     /translation="MQFDVRQIINKSQLPSKGNFKAYTDWYGFVWFSTFNGLIRYDGF
                     NCKYYTYNPTNKNSIQSNKINVVKQISHDSLLIGTNNAGLLLYSYKADAFTPITFNNI
                     DKQIMPEIIWDIDIDKNQDIWITTLNGIIHTTKNFTIKTIVNSKIAGNTPADISDFII
                     SSNNEKYVLLRDFGLYKYSHNTLIKIYPTSNSIKQQYLQIVQKNDSRLVLCASTEGII
                     ELDKNGSVKKKININNNTHASVLIDTNGYFIGLLNGGLFYYDFATQLTNEIILEKRNK
                     ELKRINQLKNDFLGVVAHDLKNPLSIINGYTQLLLM"
BASE COUNT          733 a          268 c          296 g          682 t
ORIGIN      
        1 tattaatgct ataggtttgt gaccggctgc ctgaaaatgt ttaagcatca ttaccccaac
       61 taaatgacca atatgtagag aatcggcagt agggtcaata cctaaatatg cagaagttag
      121 ttctttttgt aattgttctt cggttcccgg cattaccgtg tgaatcatgt ctcgccatgt
      181 aagttcttgt acgaaattca tattttttta aaatttgcac aaatatacat ttttttagaa
      241 atattgatag aatggctaat tcaatacttg ctttatgttc tatatttgcc atagttattg
      301 tttgttgaaa tgaaggtatt tacaattcaa aaagattaca aagtttacac cattacaaaa
      361 ccacaaaatg taaaagcatt attgtttgta atgtttattg ggggtattta tttttcattt
      421 ctgtttgtat cgggtatttc tttgattttg acttttttag cagttttttt tatgtaacac
      481 acaacggtac tatactggat ttggaacaca agcgatatac aaaaccccgt atgtgtagag
      541 gcaaagcgta tggaacatgg tttgatttac ccgatgttac atatgtgtct gtttttcaag
      601 caaaaatggt acatacatta catagtgtaa cctaccgaat atttgaaacc gaagaaaaag
      661 aatataccat aaactttata tacgacaaac gcaaacgctt gcaagtatat agctccaaag
      721 ataaagaata tagtatagaa ttggcaaaac acatagctca caaattagga gtaaagctat
      781 ataatgctac caacactcat ggtgaatggt gcgatgagct ttaggtattt atttcacgca
      841 tttcggttgg actaactccg gtcatttttc agttagaaca accaatattt taaccaattt
      901 agaaccgggt aatgggtatt ttttgaagaa ataacaatat atttgtatat aatttatatg
      961 ttgcataggt gtgtatttat aaaaaaataa ttggtctaat attaattcta tatatagtac
     1021 aaagtcacat ttgtgctcaa gatttgcagt tcgatgtaag acaaataatt aataagtctc
     1081 aattaccttc taaaggaaat tttaaagcat atacagattg gtatggtttt gtatggttta
     1141 gtacattcaa tggacttatt cgttatgatg gttttaattg taaatactat acatataatc
     1201 caaccaataa aaattcaata caatcaaata aaattaacgt agttaaacaa attagtcatg
     1261 attctttgtt aataggtaca aacaatgcag gactcttact ttattcatat aaagcagatg
     1321 cttttacgcc tatcactttt aacaatattg acaaacaaat aatgcctgaa attatttggg
     1381 atattgatat agataaaaat caggatattt ggattactac tttgaatggg attattcata
     1441 caacaaaaaa tttcacaatt aaaacaattg taaattctaa aattgcagga aatacaccgg
     1501 cagatatttc agattttata atttcatcaa acaacgaaaa atatgttttg ttaagagact
     1561 ttggtcttta taaatattca cataatacac ttatcaaaat atatcctaca tcaaatagta
     1621 ttaaacaaca atacttacaa atagtgcaaa aaaatgattc tagacttgtt ttgtgtgcat
     1681 caacagaagg tattatagaa cttgacaaaa acgggagtgt caaaaaaaag ataaatatta
     1741 ataataacac tcatgcttct gtactcattg atacaaatgg atattttata ggacttttaa
     1801 atggaggatt attttattat gactttgcta cgcaacttac aaatgaaata attcttgaaa
     1861 aaagaaataa agaattaaaa aggataaatc aattaaagaa tgatttttta ggtgttgtag
     1921 cacatgattt aaaaaatcca ctttcaatta taaatggtta tacacaatta ttattaatg
//
LOCUS       DAOTOW010000449         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44634_length_1978_cov_3.681747, whole genome shotgun sequence.
ACCESSION   DAOTOW010000449 DAOTOW010000000
VERSION     DAOTOW010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44634_length_1978_cov_3.681747"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="P5243_08045"
     CDS             <1..1245
                     /locus_tag="P5243_08045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HRS19440.1"
                     /translation="ADDFITKPIREHVFVSKIKALLQKNEIQKQNIQHFVYKALDSSQ
                     LHVLYCTGYETTFAEKIFGNTDFTYTVLYNSKELFDALYSLQHSIVCIDESATWVFQR
                     FQRFMQALDASVPVFLFVSEGIQFPIVDSNLGVLYKHFEPSYIIQQILFVNSLLQRNK
                     SQYIINLKKALLRSSFLFERIKEIDCGKFTVSVLHENYNDIPGGDFYEVFQIHDEYTL
                     ICMGDIMGKSWGAWMYVPIYLAYIRSTIKFITSRNISKIIKTPEKILQMLNQYCSKDL
                     QLSEVFTTLSVVVIENNGDNVYMSSAGAISPLLYSNNIVSTIEINGNLLGIDVNSQFE
                     GKEIQCQSGSVIVLYTDGYSEAIEIPTGKIVGHQKMQQIFQTSLNNSPRFTTLDFETA
                     YLNMCEIQKFNDDRSLLVISRK"
     gene            complement(1197..1862)
                     /gene="nth"
                     /locus_tag="P5243_08050"
     CDS             complement(1197..1862)
                     /gene="nth"
                     /locus_tag="P5243_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRS19441.1"
                     /translation="MILNEKYSSIISIFKSNLPNPTTELQYNSPFELLVAVILSAQCT
                     DKRVNQITPILFKELPTPFDFANSSVEHIYSLIKSCSYPNNKAQHLHSMATILIRQFN
                     GIVPNTIEELQMLPGVGRKTANVIVSVLYNHPAMAVDTHVYRVSKRIGLTPQAKTVRH
                     VEDILTQYTPQHEIHNMHHWLILHGRYTCTARNPKCNECKISNYCDYFRDITNKDLSS
                     LNF"
BASE COUNT          675 a          262 c          338 g          703 t
ORIGIN      
        1 gctgatgatt ttataacaaa acctatacga gagcatgtgt ttgtgtcaaa aataaaagca
       61 ttgttgcaga aaaatgaaat acagaaacaa aatatacaac attttgtata taaagcactt
      121 gattcttcgc agttgcatgt gttatattgt accggttatg aaacaacgtt tgcagaaaaa
      181 atatttggaa acactgattt tacatataca gtattatata attcaaaaga attgtttgat
      241 gccttgtatt cattacagca cagcattgtt tgtattgatg aatcagcaac gtgggttttt
      301 caacgttttc aaaggtttat gcaagcatta gatgcttcgg tacctgtgtt tttatttgtg
      361 tcagaaggta ttcaattccc tattgtagat tcaaatttag gcgtgttata taaacatttt
      421 gaaccgtcat atattataca acaaatactc tttgtaaatt ctcttttaca acgtaacaaa
      481 tctcaatata tcattaattt aaaaaaggca ctattacgct catctttttt gtttgaacga
      541 attaaagaaa ttgattgtgg aaaatttaca gtttcagttt tacatgaaaa ttataatgat
      601 attcccggcg gagattttta tgaggtgttt cagatacatg atgagtatac gcttatttgt
      661 atgggcgata taatgggaaa atcatggggg gcatggatgt atgtgccaat ttatttggca
      721 tatattagat caacaattaa gtttattact tcacgaaata tttcaaaaat aattaaaact
      781 cccgaaaaaa ttttgcaaat gctcaatcag tattgttcta aagatttgca attatctgaa
      841 gtttttacta cattatctgt agttgtaata gaaaacaacg gtgataatgt ttacatgtca
      901 tctgccggag caatttctcc attgttatat agtaataata tagtttcaac tatagaaatt
      961 aatggcaatt tgcttggtat tgatgtaaac tctcaatttg aaggcaaaga aattcaatgt
     1021 caatcaggat cagttatagt tttatatacc gatgggtata gtgaagcaat agaaattcct
     1081 accggtaaaa ttgtaggaca tcaaaaaatg caacagatat ttcaaacgtc tctcaataat
     1141 tcaccacgtt ttaccacatt agattttgaa acagcatatt taaacatgtg tgagattcaa
     1201 aagtttaatg atgatagatc tttattggta atatctcgaa aatagtcgca ataattagaa
     1261 attttacatt cattgcattt ggggtttctt gctgtgcaag tatatcttcc gtgtaaaata
     1321 agccaatgat gcatattgtg gatttcatgt tgtggcgtat attgtgtcaa aatgtcttca
     1381 acgtgtctta ccgtttttgc ctgaggagtc aatccaattc gttttgaaac tctatataca
     1441 tgagtgtcaa cagccatagc cggatgatta tagagtacag aaacaattac gtttgctgtt
     1501 tttctaccaa ctcccggaag catttgtaat tcttctatag tgttgggtac aatcccatta
     1561 aattgtctta taagtatagt tgccattgaa tgtagatgtt gggctttatt gtttggatat
     1621 gaacaagatt ttataagact atagatatgt tcaacactag agttagcaaa atcaaatggc
     1681 gtaggaagtt ctttgaaaag aataggtgta atttgattaa cacgtttgtc ggtgcattgt
     1741 gccgacaaaa taacagcaac aagtaattca aatggagaat tatattgtaa ttctgttgtc
     1801 ggattaggta agtttgattt aaaaattgaa atgatagagc tatatttttc gttcaaaatc
     1861 atagtaattt ttattcaaag ttagcaaaaa tttggtgtgg ttattgtatt ttataaataa
     1921 tagcattatt gtccgataaa actacaaaat taatttaata aaacaaaatt tatttgat
//
LOCUS       DAOTOW010000450         1976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44708_length_1976_cov_2.581468, whole genome shotgun sequence.
ACCESSION   DAOTOW010000450 DAOTOW010000000
VERSION     DAOTOW010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44708_length_1976_cov_2.581468"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1976)
                     /locus_tag="P5243_08055"
     CDS             complement(<1..>1976)
                     /locus_tag="P5243_08055"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19442.1"
                     /translation="AAISGATSATYTPPAGLTTTTSYRRYAKDNTCNTTPTVSTGTWT
                     VTVNPIPTISGTTSAARCGTGTVSLGATASSGTINWYAASSGGSSLGTGTSFNTPSIS
                     STTTYYVDATDNGCTTASRTAVIATITAIPVVTVTNGARSGNGTVNLGASTSGGTLSW
                     YDVAVGGSSLGTGTSFTTPSLTNSTTYYVEAVDNGCATREPVQAIVYKIFYSNNADIA
                     LPNNWYPNTTASVNIHPNFNDADITLIVQSGHVCPISSNVTLGSGVILQVDGTIQPLP
                     NVVITGAGIIRGSGTIHVTRTASPADFNTQYVFATKELSNLSFNYSGSGNQIINQLSV
                     SYKHLIVSGSGIKTLENSISIAQTLSFDAAVFIDLNGNTITMQNWANGNIFGHNTDRY
                     IVLNGGKIIAEGVSINEIVQFPIGISTNTIDFCRVDITNKTGTHTFTIDGVCEALYSD
                     GTCTGTNGGYVATKKAVDYTWFISSASTNAIVTFYWHTTKELPEFTSSLCAPFHYGSE
                     WELKGVPNEAGVYNTNYRYISGTFTSFSPGSVQNSEGLLPIELSSFVALKKQNGVQLE
                     WVTESEKNNEFFTILRSNNGVNFKPIATVKGSGTSSIINKYSYFDNSIYYGVTYYRLM
                     QTDFDGQQSFSTIVSVEQEQKNFDILSYTQSIGL"
BASE COUNT          601 a          369 c          305 g          701 t
ORIGIN      
        1 tgtaatccta tagattgtgt atatgatagt atatcaaaat ttttctgttc ttgttctaca
       61 cttactattg tagaaaaact ttgttgtccg tcaaaatccg tctgcattaa tctataatac
      121 gttactccat aatatatact attatcaaaa tatgaatatt tatttattat agaacttgta
      181 cctgaacctt ttaccgttgc aattggttta aaatttacac cgttatttga tctaagaatc
      241 gtaaaaaatt cattgttttt ttcactttca gtaacccatt ccaactgaac tccattttgt
      301 ttttttaatg cgacaaaaga actcaattca ataggtaata atccttcaga attttgaact
      361 gaccccggag aaaaactcgt aaatgttcct gaaatatatc tataattagt attataaact
      421 cctgcttcgt taggaacacc tttcaattcc cattcagaac cataatgaaa tggagcacat
      481 aaactcgaag taaattcagg taattctttt gtggtatgcc aatagaatgt tacaattgca
      541 ttagtacttg ctgaagaaat aaaccacgta taatctacag cctttttggt tgctacatat
      601 ccaccatttg ttcctgtaca tgttccatca gaatacaatg cttcacaaac tccatcaatt
      661 gtaaaagtat gtgttcccgt tttatttgta atatctactc tacaaaaatc aatggtattt
      721 gttgaaattc ctattggaaa ttgaacaatt tcatttatac taactccttc ggcaataatt
      781 tttcctccat tcaatactat atacctatct gtattatgtc caaaaatatt accatttgcc
      841 caattttgca tagttatagt atttccattt aaatcaataa atactgctgc atcaaatgat
      901 aaggtttgag ctattgaaat agaattttct aatgttttta ttccggatcc tgatactata
      961 agatgtttat atgaaaccga caattgattt attatctgat taccagaacc actatagtta
     1021 aatgacaaat tgcttaattc tttagttgca aaaacatatt gtgtattaaa atctgccggt
     1081 gaagctgtac gtgttacatg tatagtacct gatcctcgaa ttattcctgc tccggtaatt
     1141 accacattgg gtaaaggttg aatagtacca tctacttgta atataacacc acttcctaaa
     1201 gttacatttg aagaaattgg acacacatgg ccactttgaa ctattagagt aatatcagca
     1261 tcattaaaat tcggatgaat atttacactt gccgttgtat ttggatacca attatttggt
     1321 aatgctatat cggcattatt cgaataaaat attttgtata ctattgcttg aacaggttct
     1381 cgagttgcac aaccattatc aactgcttct acataataag tagtagaatt agtaagtgat
     1441 ggtgttgtaa atgatgttcc tgttcctaac gaacttccac ctacagctac atcataccaa
     1501 gaaagtgttc ctccagatgt tgaagctcct aaattaactg ttccatttcc tgatcgtgca
     1561 ccgttagtaa ctgtaacaac cggaattgca gtaattgtcg ctattactgc tgttctactt
     1621 gctgttgtac atccattgtc cgttgcatct acataatacg ttgttgtact tgatatactc
     1681 ggagtgttaa atgatgttcc tgttcctaac gaactaccac cacttgatgc tgcataccaa
     1741 ttgatagtac ctgatgatgc tgtagcacct aatgataccg tacctgtacc acaacgtgca
     1801 gctgatgttg tgcctgatat tgtgggtata ggatttaccg ttactgtcca tgtccctgtg
     1861 gataccgttg gagtagtatt acacgtatta tctttagcat aacgacgata actcgttgtt
     1921 gtggttaatc ctgccggtgg agtatatgtg gctgatgttg cgccgcttat ggccgc
//
LOCUS       DAOTOW010000451         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44789_length_1973_cov_3.366528, whole genome shotgun sequence.
ACCESSION   DAOTOW010000451 DAOTOW010000000
VERSION     DAOTOW010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44789_length_1973_cov_3.366528"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="P5243_08060"
     CDS             <1..232
                     /locus_tag="P5243_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS19443.1"
                     /translation="CEIWIYDLWGNLVWYSNEVENGIFVGKWDGTYNGELLKADTYIW
                     KMEAELLNGQSWNGKEAANGKISKFGSVALIR"
     gene            complement(427..1320)
                     /locus_tag="P5243_08065"
     CDS             complement(427..1320)
                     /locus_tag="P5243_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19444.1"
                     /translation="MFLLATIGTFFVTVAQTSKNDGDLDSIVYYLLFQDSIAYNDFVI
                     PKNNYDYIYTTGFFNSQSYYHGRIINTPQWNIIPQLSYLLHNGIGINAVGLWYQTYTP
                     HWSYTNISINYFSYLDKKNAFHCLIGYSYYIYSDGSELLRNSIDASIGYRLKKPKIGT
                     KFTTNISFGDGQTFEIANKTYAHIPIKKNSIYIRPQISINYAELSYITELNEIKYTYS
                     FVNTQLDIPLRIILQNFDFEIGYYYNMPIEQSFESKLPNTKALHASIGYVFPIKRPLK
                     SQNESSSSQLIQERIQSNTKL"
     gene            complement(1368..1865)
                     /locus_tag="P5243_08070"
     CDS             complement(1368..1865)
                     /locus_tag="P5243_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19445.1"
                     /translation="METITNLTKKKALHILLIAGIMLLPIANFAQTKEQNEQKSQVQT
                     FEQTLTKEQKELLQKERELMKQQREAFVKTLTEEQKQILRDNSKTQEQKREQLHATLS
                     VQQKQMLEANEQRLQQSREQFRQSLNEQQRNMYMHQNREMKGNKNQNSPENGNKQMRT
                     GSPKN"
BASE COUNT          618 a          224 c          330 g          801 t
ORIGIN      
        1 ttgcgagata tggatatacg atttgtgggg taatttggtg tggtatagta acgaggttga
       61 aaatggtata tttgtaggta agtgggatgg cacctacaat ggtgaattac tcaaagcaga
      121 tacctatatt tggaaaatgg aagcagaatt gctcaatggc caatcatgga atggcaaaga
      181 agcagcaaat ggcaaaatat caaaatttgg ttctgtagca ttgatacgat aatttgtaaa
      241 aagagaataa aaagtacatg gtattatatg aatttgatta ttaaggtaga tttttgagat
      301 tgtaacagac taatcttacg aaatctacca aagacaatca aattcgtata tagtttgttt
      361 ttgtgaacaa attttaagct actttattac atcaaaatca catgaattat attgattttg
      421 tgttttttac agttttgtgt tagattgtat tcgttcttga attagttgag atgaagaact
      481 ttcattttgt gattttagag gtcgttttat agggaaaaca tatcctattg atgcatgaag
      541 agccttagtg tttggtaatt ttgattcaaa agattgttct ataggcatat tataataata
      601 tccaatttca aaatcaaaat tttgtagtat aattcgcagt ggaatatcaa gttgggtatt
      661 tacaaaactg tatgtatatt taatttcatt taattctgta atatatgaca gttctgcata
      721 attaatgctt atttgaggac gtatatatat gctatttttt ttaataggta tgtgtgcata
      781 ggttttgttc gctatctcaa atgtctggcc atcaccaaaa gaaatatttg ttgtgaattt
      841 agttcctatt tttggttttt ttaaacgata tccaatactt gcatctattg aatttcttaa
      901 taattcactt ccgtcagaat atatatagta agagtaacca atcaagcaat gaaaagcatt
      961 ttttttatct aaatatgaaa aataatttat tgaaatattt gtatacgacc aatgaggagt
     1021 atatgtttga taccataagc caacagcgtt aattccaata ccattgtgaa gtaaatatga
     1081 aagttgaggt ataatattcc attgaggggt attaattatt cttccgtgat aatagctttg
     1141 tgaattaaaa aatccggtag tatatatata atcataatta ttttttggaa tcacaaagtc
     1201 attataggca attgaatctt gaaataataa ataatataca atagaatcaa gatctccatc
     1261 attttttgat gtttgtgcta cagtaacaaa aaaagttcct attgttgcta ataggaacaa
     1321 aagtattctt ttgtatatac aaaatttaca cataaacaat gtatatatta gtttttagga
     1381 gagcctgttc tcatttgttt attaccattt tcgggtgaat tttggttttt atttcctttc
     1441 atttctctgt tttgatgcat atacatgttt ctttgttgtt catttaatga ttgtctaaat
     1501 tgttcacgtg attgttgaag tctttgttca tttgcttcta acatttgttt ttgttgaacg
     1561 ctaagtgttg catggagttg ttcgcgtttt tgttcttgcg ttttagaatt gtcacgtaaa
     1621 atttgttttt gctcttctgt taaagttttc acaaaagctt cacgttgttg tttcataagt
     1681 tcacgttctt tttgcaataa ctctttttgt tcttttgtga gagtttgttc aaaagtttgt
     1741 acttgtgatt tttgttcgtt ttgttcttta gtttgagcaa aatttgcgat tggtaaaagc
     1801 ataattcctg ctattagaag gatatgtaaa gcttttttct ttgtaagatt agttattgtt
     1861 tccattgtct ttttttttaa tgttttttac taaattattt attgaagaac cgattctaca
     1921 ttatagacct ttaaatattc aaaaagtttt attgtaataa ttataatgtt aaa
//
LOCUS       DAOTOW010000452         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44790_length_1973_cov_3.347237, whole genome shotgun sequence.
ACCESSION   DAOTOW010000452 DAOTOW010000000
VERSION     DAOTOW010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44790_length_1973_cov_3.347237"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="P5243_08075"
     CDS             complement(<1..1866)
                     /locus_tag="P5243_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRS19446.1"
                     /translation="MVYFFEDKEHKVIAVDLQTTLTDETKQKLQWLFGNAKYIETQEI
                     SGNFIGPRKEMITPWSTNAVEITQNMGISGIIRIEEFIPDTATSHFDPMLQRKYNGLT
                     QDIFSISRTPDPIIEIDDIATYNIQEGLALNDEEIAYLESLPKKIGRKLTDSEIFGFS
                     QVNSEHCRHKIFNGTFIIDGQEKPYSLFKLIRQTTEEHQNRVVSAYKDNCAFIQGPRI
                     HMFIPQSHNKPDYFTIKEYESVISIKAETHNFPTTVEPFNGAATGTGGEIRDRIAGGK
                     AAFPIAGTAVYMTSYTRLQNPNRIWEQATKPRPWLYQTPKDILIKASNGASDFGNKFG
                     QPLICGSVLTFEHFENNKKYGFDKVIMLAGGIGFGKKVDSLKDEPSVGDKVVLLGGDN
                     YRIGMGGGAVSSVATGEYNNAIELNAVQRSNPEMQKRAYNAIRALGEMDNNPVISIHD
                     HGAGGHLNCLSELVEATGGIINIDALPIGDPTLSAKEIVGNESQERMGLVIQAKDIDL
                     LKEIADRERAPMYVIGETTGTHQFTFINNKTGEKPIDLQLEDMFGNPPKAIMQDNCIK
                     EYHTEISYDTSKIHTYISQILQLESVACKDYLTNKVDRSVTGRIATQQCCGELQLP"
BASE COUNT          543 a          342 c          341 g          747 t
ORIGIN      
        1 gggtaattga agttctccgc aacattgttg tgtggcaatt cttccggtaa ccgatcggtc
       61 aactttgttg gttaaataat ctttgcatgc taccgattct aattgtaaaa tttgagaaat
      121 atatgtatgt attttactgg tatcgtaaga aatttctgta tgatattctt ttatgcaatt
      181 atcttgcatt attgctttag gtgggttacc aaacatgtct tccaattgta aatcaatagg
      241 tttttcacca gttttattgt tgataaaagt aaattgatgc gtacctgttg tttcacctat
      301 tacatacata ggggcacgtt ctctatcagc aatttctttg agtaaatcaa tatctttagc
      361 ctgtattaca agccccattc gttcttgcga ttcatttcca acaatttctt ttgccgaaag
      421 ggtagggtca cctattggga gtgcatcaat attaataata cctccagtag cttctactaa
      481 ttccgaaagg caattgaggt gacctccggc accgtggtca tgtatagaaa ttacaggatt
      541 attgtccatt tcacctaatg cacgtattgc attataagct cgtttttgca tttcggggtt
      601 acttctttgt acagcgttta attctattgc attgttatat tcacctgttg ctacagaaga
      661 tacagctcct cctcccatgc ctattctgta attgtcgcca ccaagcaata caactttatc
      721 tcctacacta ggttcgtctt tgaggctgtc aacttttttt ccgaatccta taccgcctgc
      781 tagcattatt actttatcaa aaccatattt tttgttgttt tcaaaatgct caaatgttag
      841 aacgctgccg caaataagag gttgaccgaa tttattacca aaatcgctgg ctccgtttga
      901 tgctttgatt aaaatatctt ttggcgtttg atataaccag ggtcttggtt ttgtagcttg
      961 ttcccatatt cgattaggat tttgtaaacg ggtatatgag gtcatatata cagcggtacc
     1021 agctataggg aatgctgctt taccacctgc aatacgatca cgaatttcac ctccggttcc
     1081 tgtagctgct ccgttaaaag gttctacagt ggtagggaaa ttgtgtgttt cggctttaat
     1141 tgaaattacc gattcatatt cttttatggt aaaataatcg ggtttattat gtgattgtgg
     1201 aataaacatg tgaatacgag gaccttgtat aaatgcacaa ttgtctttgt aggctgatac
     1261 cacacggttt tggtgctctt cggttgtttg acgtataagt ttaaataatg aatatggttt
     1321 ttcttgtccg tctataatga aagttccatt aaatatttta tgacgacagt gttctgaatt
     1381 tacctgtgaa aaaccaaaaa tttcactatc ggtaagtttt ctgccaattt ttttcggaag
     1441 agattctaaa tacgcaattt cttcatcatt tagtgctaat ccttcttgaa tgttatatgt
     1501 tgcaatatca tcaatttcaa taattggatc aggagtacga gaaattgaga aaatgtcttg
     1561 tgttaatcca ttgtattttc gttgcaacat tgggtcaaaa tgtgaagttg ctgtatccgg
     1621 tataaattct tctatgcgaa taattcctga aattcccata ttttgggtaa tttctacagc
     1681 atttgtactc catggtgtaa tcatttcttt gcgaggacca ataaaattac cggaaatttc
     1741 ttgcgtttct atatatttag cattaccaaa taaccattgt aatttttgtt ttgtttcatc
     1801 tgttagtgtt gtttgtaaat ctacagctat tactttatgc tctttatcct caaaaaaata
     1861 aaccatgtgt tctaaaaatt aattgtttga attcggtaca aatatacatt tttatataat
     1921 taacccgttg tgcatttagg caatatttat tttaagatta tttgaatttc tat
//
LOCUS       DAOTOW010000453         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44820_length_1972_cov_3.888367, whole genome shotgun sequence.
ACCESSION   DAOTOW010000453 DAOTOW010000000
VERSION     DAOTOW010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44820_length_1972_cov_3.888367"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..598)
                     /gene="secG"
                     /locus_tag="P5243_08080"
     CDS             complement(266..598)
                     /gene="secG"
                     /locus_tag="P5243_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRS19447.1"
                     /translation="MGSVLINILIIATSILLVLIVLVQNSKGGGLASNFSSSNQIMGV
                     RKTTDFLEKATWVLAIALVVFCIVATTLLPKQTTIEKRSFFEDNVPENAAQVPQGIQT
                     AEGVQGEQ"
     gene            complement(603..>1972)
                     /locus_tag="P5243_08085"
     CDS             complement(603..>1972)
                     /locus_tag="P5243_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS19448.1"
                     /translation="EFPYFQTAHLLYVRGLKNNKSIRFNNQLKIAATYAGNRAKLYEL
                     LHIEDTIIVDQIETVQTIHPQAAIADEVATITQDCNIDQETSIIEIIETKSIENTSVN
                     QNNEQFIVEPEYTNTIKNYVTTQEETITKDDISAIEKTEIPIQNIEFRSLEDIELDDD
                     VYELPNNTTIENDVTGNSVEILLDSTQKESVNSSIPSISVEDSTEKTSETHIELTETI
                     EQQEPVLKQLSEKEQPTQELSLADIILQRIQKIKEQESQNNTNKERPLFSIHDITEHN
                     TAETTPIQTNTHIQIETSTKNDEPTIDTLPSSSINSTDNTIEQQDLLSFSFTEQVDET
                     PEETTNIPELEKNYAVSTEMWFKDEELQFETFDQNNLIDKFLAREHTIKPDLNKAIEN
                     QEDISQQSVKEGEYLSETLAKIYVQQKNFDKAIQIYKKLSLKYPQKSIYFANQIIELE
                     KKLKE"
BASE COUNT          558 a          260 c          309 g          845 t
ORIGIN      
        1 ctaagacctt tcctgcaagt ggtcttcctt taatttctgc catatgtcaa aatatttaat
       61 tgttaaacac tgtaattttt ggcacacgca gtttcatttt ttatgccatt gagttttttt
      121 taaaaaaaat tgaatttttg tcagttttaa aatagctaaa acaaagatag ggatgcttaa
      181 aatagtaatt aactgctatt tctgtgataa aaaaaatgtc agcatatatg acatgctgac
      241 attttttaca tatatataat ttttattatt gttctccttg tactccttca gctgtttgaa
      301 taccctgtgg aacttgtgct gcattttcgg gtacattgtc ttcaaagaat gaacgttttt
      361 caatagtagt ttgtttaggt aacaatgtag tagcaactat acaaaataca actaaagcta
      421 tagccaaaac ccatgttgct ttttctaaaa agtcggttgt tttacgaaca cccataattt
      481 gatttgatga tgaaaaattt gatgctaaac cgccaccttt tgaattttga actaatacaa
      541 ttaatacaag taaaatactt gttgcaatta ttaaaatgtt tattaaaact gagcccatat
      601 tgttattctt ttaatttttt ttctaattct ataatttggt ttgcaaagta aatacttttt
      661 tggggatatt tcaaacttaa ttttttatag atttgaatag ctttatcaaa atttttctgt
      721 tgcacatata tttttgctaa tgtttctgat aaatattcac cttcttttac actttgttgc
      781 gatatatctt cttggttttc aatagctttg tttaaatccg gttttattgt atgttcgcga
      841 gcaagaaatt tatctattaa attattttgg tcaaatgttt caaattgtaa ttcttcgtct
      901 ttaaaccaca tttcggttga taccgcataa tttttttcaa gttcagggat atttgttgtt
      961 tcttccggtg tttcatcaac ttgttctgta aacgaaaatg ataataaatc ttgttgttca
     1021 attgtattat cagttgaatt gatagaagat gaaggaagag tatctatagt tggctcatca
     1081 tttttagtag atgtttcaat ttgtatatgg gtattagttt gtataggtgt agtttcagcc
     1141 gtattgtgtt cggtaatgtc gtgaatacta aatagtggtc gttctttatt tgtattgttt
     1201 tgactttctt gttctttaat tttttgtata cgttgtaaaa taatatcggc taatgatagt
     1261 tcttgagttg gttgttcctt ttctgacaat tgttttaaaa caggttcttg ttgctcaata
     1321 gtttctgtca attctatgtg tgtttctgat gttttttcag ttgaatcttc aacggaaatg
     1381 ctaggtattg aactatttac actttctttt tgtgttgaat ctaaaagtat ttcaacagag
     1441 ttaccggtaa cgtcattttc tatagttgta ttgtttggta attcatatac atcgtcgtca
     1501 agttctatgt cttctaatga acggaattca atgttttgta ttggaatttc agttttttca
     1561 atagcagaga tatcatcttt tgtaatagtt tcttcttgag ttgttacata attttttata
     1621 gtattagtat attcaggttc tactataaat tgttcattat tttgattaac agaggtattt
     1681 tctattgatt ttgtttcaat tatttcaata atcgaagttt cttggtcaat attacaatct
     1741 tgtgtaattg ttgctacttc atcggctatt gccgcttgtg gatgaatagt ttgaacagtt
     1801 tctatttgat ctactataat tgtatcttca atgtgtagca attcatacaa ttttgcacgg
     1861 ttaccggcat atgttgctgc tattttaagt tgattattga aacgtatgct tttattattt
     1921 ttaagaccgc gaacatataa aagatgtgca gtttgaaaat acggaaattc tt
//
LOCUS       DAOTOW010000454         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_44995_length_1967_cov_3.583682, whole genome shotgun sequence.
ACCESSION   DAOTOW010000454 DAOTOW010000000
VERSION     DAOTOW010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44995_length_1967_cov_3.583682"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="P5243_08090"
     CDS             <1..371
                     /locus_tag="P5243_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRS19449.1"
                     /translation="TAVSKQISLPKSYDEEILEKTMNCIHTHISNPNLSIDDISEYVC
                     VSKIQLYRKIKAITGLTPADIVKTTRLQVAEEMVVSQKYTITEICFYVGFSDPKYFSK
                     CFKEQYGVSPSEYVKKKTSI"
     gene            511..>1967
                     /locus_tag="P5243_08095"
     CDS             511..>1967
                     /locus_tag="P5243_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HRS19450.1"
                     /translation="MKPISYIKIFLAISIVSLSQLLYATKLVEVKVVDKDHIMLYFLD
                     GEISYPENLTQLGTRAYTNNTSADDNALVTYGTALNTTNAATVANWTIKSSDDANFGT
                     AGKNPTNIYRKSKLSGVSQEAWNTATNDYNYDWAYEHTIFLKLPFSMVQGKTYTVQLN
                     ANINSDVNTKTFTYDIFSSKSEAIKVNIAGYLSSPSIKAADVYMWMGDGGARDYTSFQ
                     GNKVYIYNVQSGVSTEVGTLSYWKAAAAEFSDGHQMIRSAVWKADFTGFNTPGTYRLA
                     VAGIGCSEEFTISADAYLNPFKVSTLGFFYMRIGESANGTSPVPRRPLWIPNTSPTNC
                     EVIITTMHPYHPNWNTFCNGDKWDCKDEWATYATTRKNPNAYGGHSDALDWDRHLGHV
                     PIIYDMLLPYFISNGALSEDNLGIPESGNGIPDILDEARNEVDFWLRLRDGKGYSHGL
                     NNPNGTNKLYQAGNTAVAAWANAANAAMLANCFLIA"
BASE COUNT          687 a          309 c          358 g          613 t
ORIGIN      
        1 aaacagcagt aagcaagcaa atatctttac caaaatcata tgatgaagaa atacttgaaa
       61 aaactatgaa ttgtattcat actcatattt caaatcctaa tttatcaata gatgatataa
      121 gcgagtatgt ttgcgtaagt aaaattcaat tgtatagaaa aattaaagca attaccggtt
      181 taactcctgc cgatattgta aaaacaactc gtttacaagt tgcagaagaa atggttgtat
      241 cacaaaaata taccattacc gaaatttgtt tttatgtagg attttccgac ccaaaatatt
      301 ttagtaaatg tttcaaagaa caatatgggg tatcaccttc ggaatatgtc aaaaaaaaga
      361 caagtatttg aaaattatac aaatatcagt tactgagaaa ttataattga taaaaaatta
      421 catctatttt gatacattac tttaccatat actatagatt gattttgtag ttttgtatag
      481 tgagagttat accttaaaaa ataaataaat atgaaaccta tttcctacat caaaattttt
      541 cttgcaatta gcattgtaag tctatcacag ttattgtatg caacaaaatt agttgaggta
      601 aaagttgttg acaaagatca tataatgctg tattttttag atggtgagat aagttatcct
      661 gaaaatctta ctcaattagg tactcgtgca tatacaaata acacaagtgc cgacgataat
      721 gctcttgtaa cctacggcac ggcacttaat acaacaaatg ctgctactgt agctaattgg
      781 acaataaaat catctgatga tgctaatttt ggtactgcag gtaaaaatcc tacaaatata
      841 tatcgcaaat ctaaactttc tggagtatcg caagaagcat ggaataccgc aaccaacgat
      901 tataattatg attgggcata tgaacataca atttttctga aattaccttt ttcaatggta
      961 caaggaaaaa catataccgt acaattgaat gccaatataa attcagatgt aaacacaaaa
     1021 acatttacat atgatatatt ttcgtcaaaa tctgaagcta ttaaagtaaa catagcagga
     1081 tatttatcgt cgccaagtat aaaagcagct gatgtatata tgtggatggg cgatggggga
     1141 gcacgtgatt atacaagttt tcaaggaaac aaagtatata tatataatgt tcaatcggga
     1201 gttagcaccg aagtcggaac tctttcatat tggaaagcag cagcagcaga attttctgat
     1261 ggtcatcaaa tgatacgtag tgctgtgtgg aaagctgatt ttaccggctt taacactccc
     1321 ggcacatatc gtttagctgt tgcgggaata ggttgcagcg aggaatttac aatttctgcc
     1381 gatgcatatt taaatccttt taaagtgagt acattaggtt tcttttatat gcgtataggc
     1441 gaaagtgcta atggcactag tcctgttcct cgtcgtccat tgtggattcc aaatactagt
     1501 cctacaaatt gcgaggttat aattacaaca atgcatccat accatcctaa ttggaataca
     1561 ttctgcaatg gcgataaatg ggattgtaaa gacgaatggg ctacatatgc cactacgcgt
     1621 aagaacccaa atgcgtatgg cggacatagt gatgcgttag actgggatag gcatttagga
     1681 cacgttccca taatttatga tatgttatta ccatatttta tttcaaatgg agctcttagt
     1741 gaagataact taggtattcc cgagagtggt aatggtattc ccgatatact tgatgaagct
     1801 cgtaacgaag ttgatttttg gttgcgatta cgcgatggaa aagggtatag tcatggtttg
     1861 aacaatccta atggaacaaa taaattatac caggcaggta atactgccgt tgctgcttgg
     1921 gcaaatgccg caaatgctgc aatgcttgct aattgttttt taattgc
//
LOCUS       DAOTOW010000455         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45010_length_1967_cov_2.544456, whole genome shotgun sequence.
ACCESSION   DAOTOW010000455 DAOTOW010000000
VERSION     DAOTOW010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45010_length_1967_cov_2.544456"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /gene="thyA"
                     /locus_tag="P5243_08100"
     CDS             complement(<1..377)
                     /gene="thyA"
                     /locus_tag="P5243_08100"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRS19451.1"
                     /translation="MQQYLDLLSKVLNEGVQKHDRTGTGTISLFGHQMRFNLQEGFPL
                     LTTKKLHTRSIFIELLWFLKGDTNIKYLKDNNVRIWDEWADANGDLGPVYGYQWRKWP
                     DYKGGHIDQISQLIDSIKHNPDSR"
     gene            complement(465..650)
                     /locus_tag="P5243_08105"
     CDS             complement(465..650)
                     /locus_tag="P5243_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19452.1"
                     /translation="MDTNKKINNAQSGAIYGLGFIGAAVYFILKSTCFWGGIWGVIKA
                     IVWPAILVFEAFKTFGI"
     gene            complement(756..1898)
                     /locus_tag="P5243_08110"
     CDS             complement(756..1898)
                     /locus_tag="P5243_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19453.1"
                     /translation="MERIFSFIMLLCTICGIIVVWREYTKPYNLQAYAWLPVLLWIII
                     PDIIWTFSNNLLETAISPLVLFSIYFFTKSIHSKTLLFITLGSICLWIAYGIKGPVAL
                     FPLSFFIIHSILFKQLTISKAIRNTIIAFSIVCILFILLCLIEPRFVHSFTIYHSIQV
                     FAALRGNNEITVSWRGAILVNLFISLLPLFVITIVFLFIQKIRTKQIAKKRTHNQVFL
                     FFILLFVSGSLPLVITLKQRIFYAVPSYSFITIACALLLVHGIPTLSVKSRHITAIIG
                     IIGVCFGICFTIYTNNTYKKDAVLLQDIATIHTHIPPHTIIGCTQELTRNWKLLAYLQ
                     RFASISITDTRKDLPYIIVEKHKAFQNSEYKPYILLQTLTVYKKIT"
BASE COUNT          714 a          291 c          310 g          652 t
ORIGIN      
        1 cgcgaatcgg ggttatgttt tatagaatca attaattgag atatttggtc aatatgccca
       61 cctttataat caggccattt tctccattga tatccatata ccggacctaa atcaccattt
      121 gcatctgccc attcatccca aatacgtacg ttgttatctt tgagatattt aatattggtg
      181 tcgcctttga ggaaccacaa caattcaata aaaatagaac gagtatgtaa ttttttggta
      241 gttaatagag gaaaaccttc ttgcaaatta aaacgcattt ggtggccgaa taaactaata
      301 gttccggtac ctgttctatc gtgtttttgc actccttcgt ttaaaacttt tgagagcaaa
      361 tctaagtatt gttgcataat tgaattgttt atacaaacat acaaaaactc aacaaactat
      421 aagaatatag caaaagagaa ttattaacac tcaaataaca aagcttatat cccaaatgtt
      481 ttgaatgctt caaatactaa tatggcaggc caaactattg ctttaataac accccatatg
      541 ccaccccaaa aacaagttga tttaagtata aagtaaacag cggctcctat aaagcctagt
      601 ccatatatag caccggattg tgcattgtta atttttttat ttgtatccat aggtatttac
      661 aatgtgtggt ttatcaagaa ttctcgttag gtattatttc aaatataccc attttttaca
      721 aaataaaata atgaatatta ctatttgtat ataaactatg ttattttttt ataaacagta
      781 agggtttgca aaagtatata tggcttatat tccgaattct gaaaggcttt atgtttttca
      841 acaattatat atggtaagtc tttacgggta tctgttattg aaattgaagc aaaacgttgt
      901 aaatatgcca gtaatttcca attacgcgta agttcttgag tgcagccaat aattgtatgt
      961 ggaggaatgt gagtatgaat agtagcaata tcttgtaata aaacagcatc ttttttatat
     1021 gtattatttg tatatattgt aaaacatatt ccaaaacaaa ctccaataat tccgattata
     1081 gctgtaatat gtcttgattt tactgataaa gtaggaatac catgaactag taataatgca
     1141 caagctattg tgataaatga atatgaaggt actgcataaa aaattcgttg ttttaatgta
     1201 attaccaaag gcagtgaacc agacacaaac aggagtataa aaaacaggaa tacctgattg
     1261 tgagtacgtt tttttgctat ttgtttagtt ctaatttttt gtatgaacag aaatacgatg
     1321 gttattacga aaagaggtaa taaacttatg aaaagattaa caagaattgc tcctcgccat
     1381 gaaacggtta tttcattatt tccccggagt gcagcaaaca cctgaataga atgatatatt
     1441 gtaaaagaat gaacaaatcg aggttcaatt agacacaata gtataaaaag tatacaaaca
     1501 atagaaaatg ctataatggt gtttcgtatt gcttttgaaa tggttaattg tttaaataat
     1561 attgaatgta ttataaaaaa tgatagtgga aacaaagcca caggtccttt tataccataa
     1621 gctatccata aacaaatgct tcctaatgtt ataaacaaca gtgtttttga gtgaatgctc
     1681 ttggtaaaaa aatatatact aaaaagtacc aacggtgaaa ttgccgtttc aagtaaatta
     1741 ttagagaaag tccatattat atcggggatt ataatccata aaagcacggg caaccatgca
     1801 tatgcttgca aattatatgg tttagtatat tctcgccaaa ctactattat tccgcatatg
     1861 gtacagagca gcattataaa cgaaaatatt cgttccacta taaatgaatc tcctaataat
     1921 gcaaaaaaga atgattgggt actaggctgt ttttttaaaa caagtta
//
LOCUS       DAOTOW010000456         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45263_length_1958_cov_3.136626, whole genome shotgun sequence.
ACCESSION   DAOTOW010000456 DAOTOW010000000
VERSION     DAOTOW010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45263_length_1958_cov_3.136626"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1137)
                     /locus_tag="P5243_08115"
     CDS             complement(85..1137)
                     /locus_tag="P5243_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRS19454.1"
                     /translation="MTLLLVYLFLALSISFLCSIMEAVLLSTPQSFIMTKKENRWSKT
                     FLDYKKNIDKPLSAILTLNTVAHTVGAAGVGAQASIVFGDEYFGLVSAILTILILVLT
                     EIIPKTIGANYWRNLTTISAIIITSMYIVTYPLVLLSALITKIFTGKAHTMSTSREEI
                     SAMASIGADQGIFSQKEHIIIQNIIKLKNVKVSEIMTPRVVLAIADENLPISIFLQSK
                     HYLKYSRIPIYCNNEENITGYVFRQTVFEKLAEDKHDMKLKDIRRDIVIVHNSKTLFT
                     VWENLLERREHIALVVDEYGGVDGIVTMEDIIETMLGLEIIDEKDTIADMQSFARERW
                     KLREAKYSYLHQIKDE"
     gene            complement(1134..>1958)
                     /locus_tag="P5243_08120"
     CDS             complement(1134..>1958)
                     /locus_tag="P5243_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS19455.1"
                     /translation="QGSVFIEGVDINNQTITDLAKKMSVVLTDKIETVNMTVFDIVSL
                     GRFPYTNWLGKIEHTDNIIIHQSIERVGLQGFADRTIDTLSDGEKQRVMIARALAQNT
                     PFIMLDEPTAHLDLPNRIEIMKLLKELARSTYKSILLSTHELDLALQVADRIWLMQHN
                     KPIACGTPEDLVLQGDFEQAFKKQSFDFDITNGLFKIVYTFKHSVSMSGNKIYAFWTK
                     RALHRHGFAICDQQEHIPHIEIHNKQWILHTQQTTTICKSIFELLIALNSTYYKQI"
BASE COUNT          610 a          300 c          311 g          737 t
ORIGIN      
        1 gaataggaat agaaatacat atagctatag taattataat aattgatatt ttgaaaatag
       61 tatatatatt catgaatgta actgttattc gtcttttatt tgatgtaaat acgagtattt
      121 tgcttcgcga agtttccatc gttctcttgc aaaactttgc atatcggcaa ttgtatcttt
      181 ttcatcaata atttctaatc ccaacattgt ctcaattata tcttccatag taactatccc
      241 atcaacacct ccatactcgt ctactaccaa agctatatgt tctcttcgtt ccagtagatt
      301 ttcccaaaca gtaaatagtg ttttagaatt atgcacaata actatatctc gtcttatatc
      361 ttttaatttc atatcgtgtt tatcttcggc taatttttcg aaaacggttt gtctaaagac
      421 atatccggtt atgttttctt cgttgttaca ataaataggt attcgagaat attttaaata
      481 atgtttgctt tgtaaaaata ttgaaattgg taaattttca tcggcaattg caagaacaac
      541 ccgaggtgtc ataatttccg aaacttttac atttttaagt tttatgatat tttgaattat
      601 aatatgttct ttttgagaga aaattccttg gtcggcacca atacttgcca ttgccgaaat
      661 ttcttcgcgt gaggttgaca ttgtatgagc tttgcctgta aaaatttttg taataagagc
      721 tgagagtaat acaagcggat atgttacaat atacatgctt gtaataatga tagcagaaat
      781 tgttgtaaga tttcgccaat aatttgctcc tatggtttta ggtataattt cggttaatac
      841 taaaataaga atagtaagaa tagctgaaac taaaccgaaa tattcatcgc caaaaactat
      901 agatgcttga gctccaacac ctgctgcacc tacagtgtga gccactgtgt tgagtgttaa
      961 aattgcagat agaggtttgt ctatattttt tttgtaatct aaaaatgttt tgctccatct
     1021 gttttctttt ttagtcataa taaatgattg aggagtagaa agtaataccg cttccataat
     1081 tgaacacaaa aatgaaatag aaagagctaa aaacagatat acaagcaata gtgtcatatt
     1141 tgtttataat aggttgaatt taacgcaatt aacaattcaa aaattgattt gcatattgta
     1201 gttgtttgtt gggtatgtaa aatccactgt ttattgtgaa tttctatgtg cggaatatgt
     1261 tcttgttgat cgcagatggc aaaaccatgt ctgtggaggg ctcgttttgt ccaaaaagca
     1321 tatattttgt tgccgctcat agaaacagaa tgtttaaatg tgtatacaat tttaaacaat
     1381 ccatttgtaa tatcaaagtc aaatgattgt tttttaaatg cttgttcaaa atcaccttgc
     1441 agcactaaat cttcaggagt tccacaagct attggtttgt tgtgttgcat taaccaaatt
     1501 ctatcggcaa cttgtagtgc taaatccaat tcatgtgttg aaagtagaat agatttatat
     1561 gtgcttcggg ctaattcttt taataatttc attatctcta ttctattggg taaatctaaa
     1621 tgagctgttg gttcgtcaag cattataaat ggagtatttt gagctaatgc acgtgctatc
     1681 attactcgtt gtttttctcc atcgcttaat gtgtcaattg ttctatctgc aaatccttgt
     1741 aaaccaactc tttcaattga ttgatggatt attatattgt cagtatgttc aattttacct
     1801 aaccaatttg tatatgggaa tcgtccaagt gatactatat caaatacagt catatttacc
     1861 gtttctattt tatcggtaag tactacgctc attttttttg caagatcggt aatagtttga
     1921 ttatttatat caacaccttc tatgaataca gacccttg
//
LOCUS       DAOTOW010000457         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45407_length_1954_cov_3.052659, whole genome shotgun sequence.
ACCESSION   DAOTOW010000457 DAOTOW010000000
VERSION     DAOTOW010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45407_length_1954_cov_3.052659"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1513)
                     /locus_tag="P5243_08125"
     CDS             complement(53..1513)
                     /locus_tag="P5243_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRS19456.1"
                     /translation="MSNNSPTVKFCNGQEIPIELHKVRIVQKLHLRPIDERYEAMKKG
                     GFNTFLLNTKDIFLDMLTDSGTNAMSDNQVSAMLQADDAYAGSQSYYKMEEALQEVFG
                     KQLVLPVHQGRAAENIISQAFIKPGDIIPMNYHFTTTKAHMELNGGTIFEIFTDEGLV
                     IKSSHPFKGNIDIQKLQKLIAEYGAEKVAFIRMEASTNLIGGQPFSIENMRQVYNIAK
                     QNNILMVLDASLIGENAYFIKQREAEFKNSSIKDILRTMCDMADIVYFSSRKVSSTRG
                     GGICTNNKDLYMKMRDLVTLYEGFLTYGGMSIREIEAMAVGLRETLDETVICQSPSFI
                     KYAVDELDKLGVPVVTPAGALGCHVDAGGFLPHVPQKEYPAGALAAAFYIISGCRGME
                     RGTISSVRLENGEDLLADVELLRLAFPRRVFTLSQTMFVVDRMNWLYKNKELVGGLRF
                     VEEPKVLRFFNGRLEPTSDWPQKLIAKFKQDFGDSL"
     gene            complement(1679..>1954)
                     /locus_tag="P5243_08130"
     CDS             complement(1679..>1954)
                     /locus_tag="P5243_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19457.1"
                     /translation="LPQVHAITVNTISSSPQHIAMYINTYNPHIETMEGAAVHYVALQ
                     EHIPCIHIRAVSNAVGDRDKEKWNISGAVSNLHEVIHSIIAEIENYF"
BASE COUNT          648 a          370 c          267 g          669 t
ORIGIN      
        1 aagggttttc attaatatat gaaaacccct atataacaat aattataaag atttataaac
       61 tatcaccaaa atcttgttta aatttagcaa ttaatttttg aggccaatcg ctggttggtt
      121 ccaatctacc attgaagaaa cgaagcactt ttggttcttc tacaaaacgc aatcctccaa
      181 ctaattcttt gtttttatat aaccaattca tacgatcaac tacaaacata gtttgcgaaa
      241 gcgtaaatac tctgcgtgga aaagcaagac gtaataactc tacatctgct aataaatcct
      301 caccattttc taaacgtaca ctcgaaatag ttcctcgttc catacctcta caaccagata
      361 tgatatagaa tgctgcagct aaagctcctg caggatattc tttttgtggt acatgaggta
      421 aaaatccgcc tgcatcaaca tggcacccca atgctcctgc aggagttact accggaacac
      481 ctaatttgtc aagctcatca acagcatatt ttataaatga aggtgattga caaataactg
      541 tttcatctaa cgtttctcgc aatcctactg ccattgcttc aatttctcga atactcatac
      601 cgccatatgt taaaaatcct tcatacagag taaccaaatc acgcattttc atatacaaat
      661 ctttgttatt tgtacaaata cctccaccac gtgtagaact aactttgcga cttgagaaat
      721 agacaatatc tgccatatcg cacattgttc taagtatatc tttaatgctc gaatttttaa
      781 attctgcctc acgttgtttt ataaaatacg cattttcgcc aattaaactg gcatctaata
      841 ccataagtat attgttttgt ttagctatgt tatatacttg tcgcatattt tcaattgaaa
      901 aaggctgacc acctataaga tttgtagatg cctccattcg aataaaagca actttttcgg
      961 caccatattc agctattaac ttttgcaatt tttgaatatc aatatttcct ttaaaaggat
     1021 gtgaactttt aatgactaat ccttcatcgg taaaaatttc aaaaattgtt cctccattca
     1081 actccatatg agctttggtg gttgtaaagt gataattcat aggaataata tcacccggtt
     1141 taataaatgc ttgtgatata atattttctg cagctctacc ttgatgcaca ggtaatacca
     1201 actgtttacc aaaaacttct tgtaaggctt cttccatttt gtaataactt tgagaaccgg
     1261 cgtaggcatc atcggcttgc aacattgccg aaacttgatt gtcactcata gcatttgttc
     1321 cgctatcggt taacatatca agaaaaatat cttttgtgtt taacaaaaat gtattgaaac
     1381 cacctttttt catagcctca tatcgctcat caataggacg caaatgaagt ttttgtacta
     1441 tacgaacttt gtgaagctca attggaattt cttgtccatt acaaaattta acagttggag
     1501 aattattgct catacataaa aaatataaaa aatgaatact aaatacctaa ttggttcaaa
     1561 tatagtaaaa attttaattt tgatttaata aagtataatt ttatagtatt gttaactctt
     1621 taaaaaaaaa gaattacttg aaaaaactat tttcaaaaat gtagtatgat ctactctatc
     1681 agaaataatt ttctatttct gcaattattg aatgaattac ctcatgcaaa tttgaaacag
     1741 ctcctgaaat attccatttt tccttgtccc tatctcctac cgcattagaa accgcacgta
     1801 tatgtataca aggaatatgt tcttgcaaag caacatagtg cacagctgca ccttccatag
     1861 tttctatgtg gggattatat gtatttatat acattgctat atgttgtgga ctactgctaa
     1921 tagtatttac tgtaatagca tgtacttgtg gtaa
//
LOCUS       DAOTOW010000458         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45469_length_1952_cov_3.653664, whole genome shotgun sequence.
ACCESSION   DAOTOW010000458 DAOTOW010000000
VERSION     DAOTOW010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45469_length_1952_cov_3.653664"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..588)
                     /gene="rplS"
                     /locus_tag="P5243_08135"
     CDS             complement(241..588)
                     /gene="rplS"
                     /locus_tag="P5243_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRS19458.1"
                     /translation="MNLLDEVKKEVIEKKEWPKFNSGDTITVNYKIKEGDKERIQQFR
                     GVVLQRRNEGSVETFTIRKMSGNVGVERIIPINSPMIDSIEINKKGKVRRARIFYLRD
                     LTGKKARIKEKKF"
     gene            complement(693..1895)
                     /locus_tag="P5243_08140"
     CDS             complement(693..1895)
                     /locus_tag="P5243_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19459.1"
                     /translation="MFIRYNKEIWKLLRSSLSIPQIVAFSLSACIGLFVLFFAVFCFI
                     DFTKYIKKDTVLFAKEFIIVSKKVSALQTLTQKKAHFTQREIQEIQKQEFFTQVNVFE
                     TSQFQIQLFTDSDKLPAIKTDLFFEAVPNQYLNADFIDWHWKEGQTFIPIIFPQNFLS
                     LYNYGFAPGQGLPQLSESVFTKIPLKIKIAGNGLQHIYDARVVGFSQRINTILVPQHF
                     LQWANKTYAEKHDAQVSRLIIECKNIANPKIFEYISHKNFQINDESLVNSKLAFYLTL
                     TLSIVGGIGILITLLAIWLLLFSFQFIIEKNKYTLQNLFYLGYTYSEISKPYRIFSMV
                     LTVVLLLLSLFVSNIMHNYVIQKLQILIEIPHPGILVPLITAIVSFVMIQIIHTVAIQ
                     KAVKRAVQ"
BASE COUNT          684 a          297 c          265 g          706 t
ORIGIN      
        1 caacactcat ttaataaacg atgctgatac taaaaatgta ggtttagaca cgaacaacga
       61 tggtacagac gacaaaatgg tttcgaaagt acagtttaaa caactattgg gagttggatt
      121 ttcatataag ttttaacact tataataata gaattacatt gatttaaatt aagtacaata
      181 aaaaaaaggc tgtcaataaa ataatgacag ccttttttta tattgtttag agagaaatta
      241 ttaaaacttt ttctctttaa tacgagcttt tttaccggta agatcgcgta agtaaaatat
      301 tcttgctcta cgaacttttc cttttttatt aatttcaata ctgtcaatca ttggagagtt
      361 tatcggaata attctttcaa caccaacgtt acctgacatt ttacggatag taaatgtttc
      421 tacacttcct tcgttacgac gttgtaatac aactccacgg aactgttgaa tacgttcttt
      481 atcaccttct ttaattttgt aattgacagt tatagtatcg ccactattga attttggcca
      541 ttcttttttc tcaataactt cttttttaac ttcgtctagt agattcatcg tagtaaattt
      601 attttaatcc tttcgatttt gaggctgcaa agttataatt attttttaat tttcatagtt
      661 tttacaaaaa taatttagca caaattagaa ttttattgta ctgctctctt tacagctttt
      721 tgtatggcaa cggtatgtat gatttgtatc ataacaaatg aaacaatggc tgtgatcaat
      781 ggcactaata ttcccggatg tggaatttca attaaaattt gtaatttttg aataacataa
      841 ttatgcatta tattacttac aaataatgac agtaatagca gaacaacggt tagtaccata
      901 gaaaatattc tatatggttt tgaaatttca gaatacgtat accccaaata aaacaaattt
      961 tgtaatgtat atttattttt ttctataata aattgaaaac taaacaacaa caaccaaata
     1021 gcaagcaatg taatgagtat tcctattcca cctactatac tcaatgttaa cgttaagtag
     1081 aatgccagtt tactgtttac taaactttca tcgtttatct gaaaattttt atgcgatata
     1141 tattcaaaaa tctttggatt ggcaatattt ttacactcta tgattaaacg actaacttga
     1201 gcatcatgtt tttctgcata tgttttattt gcccattgca aaaaatgttg cggaactaag
     1261 attgtattta ttcgttgcga aaaaccaacc acacgagcat catatatatg ttgtaaacca
     1321 ttacccgcaa ttttaatttt taaaggaatt tttgtaaata cagactccga caattgaggt
     1381 aacccttgcc cgggagcaaa accataatta tacaacgata aaaaattttg aggaaaaatt
     1441 attggaataa atgtttgtcc ctccttccaa tgccaatcaa taaaatcggc attgagatat
     1501 tgatttggca cagcttcaaa aaataaatct gtttttatag caggtaattt atcgctatcg
     1561 gtaaacaact gtatttgaaa ttgtgatgtt tcaaatacat ttacttgtgt aaaaaattct
     1621 tgtttttgaa tttcttgtat ttctctttgt gtaaaatgag cttttttttg tgttaacgtt
     1681 tgtaatgccg acactttttt acttacaata ataaattcct tagcaaataa aactgtatcc
     1741 tttttgatat actttgtaaa atctataaaa caaaaaacag caaaaaacaa tacaaataat
     1801 cctatacatg cagataaaga aaatgcaact atttgcggaa tactcaacga actgcgaagc
     1861 aatttccaaa tctctttatt atagcgaata aacatgcatt gatgaattaa gtgaaactaa
     1921 ttgaagagca gtaagcacaa tggtagcttg tt
//
LOCUS       DAOTOW010000459         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45631_length_1947_cov_2.851480, whole genome shotgun sequence.
ACCESSION   DAOTOW010000459 DAOTOW010000000
VERSION     DAOTOW010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45631_length_1947_cov_2.851480"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="P5243_08145"
     CDS             complement(<1..1924)
                     /locus_tag="P5243_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRS19460.1"
                     /translation="MTKEYIRLLLHASSHNFKNILAVTFTNKACGEMKERILETLFAL
                     SNNISSVVYAQDLCDEFSFTHEQLELEAKRILHEILHNYSFFYVETIDTFFQRIIRNF
                     AKELGLHSYYGLQLDVETVIQNSIQELFLHIYEYPQIQQWLIEYTNSKIDEAKERDVF
                     KSLVKDSKILLSESFMQIHDSHVAFDEISTFFKKLEQIISEFENEAQVVAKQIVTIVT
                     KHGLQSVDFKGGSRSCMNPIYAIAEGKEFDYEVAKFQKLLNHDEWFAKTSPVKNVQSV
                     LENTQMPQLLQTIFDVYAGPNAIKYRTAQLLLTNKYLVGMLQAIQQSVDLYCKEQELF
                     LISNTNKFLQTIIGDSDAPFVYEKIGCFIKHMMIDEFQDTSKMQWHNFFPLINNALAE
                     GGSALVVGDVKQSIYRFRNGDWKLLHNTIYKDFSHDVHTVSLQHNWRSRKNIIQFNNV
                     FFKVFSKTIEASFHKKIHDDGIDTIPDDMVSVIQDLYSDVTQEISNRTKDGGFVQIQI
                     VEKDSYLELVLQQLIKDVVYVLEQGYAPEDIVFLCREKKHISLLVSYFNEQKQVYPQY
                     TEAFSIVTGEGLLLSGSDAVQFILAYLQWLENPAVVFNKKLMSIIFARLYSTNSLCDI
                     SNSNDFESQLQSLSHIS"
BASE COUNT          689 a          328 c          250 g          680 t
ORIGIN      
        1 ttgaaatatg agacagtgat tgcaattgtg attcaaaatc atttgaatta ctaatatcac
       61 acaaagaatt agtgctatac aaacgagcaa atattatact catcaacttt ttattaaaca
      121 caactgccgg attttctaac cactgtaaat atgccagtat aaactgaaca gcatcagaac
      181 cggataataa taatccttca ccggtaacaa ttgaaaaagc ttcggtatac tgaggatata
      241 cttgtttttg ttcattaaaa taagacacta ataaagatat atgttttttt tcacgacata
      301 aaaaaactat atcttcggga gcatacccct gttctaaaac ataaacaaca tcttttataa
      361 gttgttgcag tactaattcc aaatatgaat ctttttctac aatttgtatt tgcacaaatc
      421 caccatcttt tgtgcgattt gaaatttctt gtgtaacatc tgaatataaa tcttgtatta
      481 cagataccat atcatcggga atagtatcaa ttccatcatc atgaattttt ttatgaaatg
      541 atgcttctat cgtttttgaa aatactttaa agaacacatt attaaattga ataatgtttt
      601 ttcgacttcg ccaattatgt tgcaatgata cagtatgcac atcatgcgaa aaatccttat
      661 atattgtatt gtgcaacaat ttccaatcac catttctaaa cctgtaaata gactgcttta
      721 catcaccaac tacaagagca ctgccacctt cggctaacgc attatttata agcggaaaaa
      781 aattgtgcca ctgcatttta gatgtgtctt gaaattcatc tatcatcata tgcttaataa
      841 agcatccgat tttttcgtac acaaaaggag catcactatc accaattata gtttgtaaaa
      901 atttattggt atttgaaatc aaaaaaagtt cttgttcttt acaatataaa tcaacagact
      961 gctgaatagc ttgtaacata ccaaccaaat acttatttgt taaaagtaat tgtgccgttc
     1021 tatacttaat agcattaggt cctgcatata catcaaatat cgtttgtaat aactgaggca
     1081 tttgcgtatt ttctaataca gattgtacat tttttacagg tgatgtcttg gcaaaccatt
     1141 catcatgatt cagaagcttt tgaaatttag caacttcata atcaaattct ttgccttctg
     1201 ctattgcata tattgggttc atgcaactac gtgaacctcc tttaaaatca acagattgta
     1261 acccatgctt tgttactatg gtaactattt gttttgccac aacttgtgct tcattttcaa
     1321 attcactaat tatctgttct aattttttaa aaaaagtaga aatttcatca aaagcaacat
     1381 gagaatcatg tatttgcata aatgattctg atagtaaaat tttactatct tttactaaag
     1441 atttaaaaac atcacgttct ttagcttcat caatttttga attagtatat tctataagcc
     1501 attgttgaat ttgaggatat tcatatatat gaagaaacaa ttcttgaata ctgttttgaa
     1561 taacagtttc cacatctaat tgtaacccgt aatacgaatg taatcctaac tctttagcga
     1621 aatttcgaat aattcgctga aaaaacgtat cgatagtttc aacataaaaa aaagaataat
     1681 tatgtaaaat ttcgtgtaaa attcgtttgg cttccaattc taattgttca tgggtaaaag
     1741 aaaattcatc acataaatct tgagcatata caactgatga aatattattt gataaagcaa
     1801 acaacgtttc caaaatacgc tctttcattt caccacaagc tttgttagta aatgttaccg
     1861 ccaaaatatt tttaaaatta tgagaagaag catgtaataa aagacgaata tattcctttg
     1921 tcaaagcgta cgttttaccc gaaccag
//
LOCUS       DAOTOW010000460         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45660_length_1946_cov_2.975145, whole genome shotgun sequence.
ACCESSION   DAOTOW010000460 DAOTOW010000000
VERSION     DAOTOW010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45660_length_1946_cov_2.975145"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="P5243_08150"
     CDS             <1..393
                     /locus_tag="P5243_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRS19461.1"
                     /translation="KKSGFKGIAGISDMRDMKQAAQQGNERAQLALDMLAYRIKKYIG
                     AYAAAMQGVDCVVFTGGIGENNPDLRKQVCSGLEFMGVYFDEQANTNSQSHEYIISQP
                     SSAITVMVIQTNEEIVIARDTYELITHN"
     gene            481..1743
                     /locus_tag="P5243_08155"
     CDS             481..1743
                     /locus_tag="P5243_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRS19462.1"
                     /translation="MASTIFGIIIAVLVFDYALERILDLLNAKHRVQELPIELQDIYN
                     AEEYAKQQQYEQTKTQFGFITASFSFILILGMLLFDGFAVIDTYIYSSFGTPQEPLNH
                     ILGGLLFFGIVMFASDILGIPFEIYSTFVIEEKFGFNKTTPKIFVFDKLKSWLLSILL
                     GGGILAALLFLISYFGNYFWVYAWIFVALIMIAITMFYSTLIVPLFNKQTPLEEGELK
                     QAIFEFSSKVGFKLDNIFVIDGSKRSTKANAYFSGLGAKKRIVLYDTLIQEMTTEEIV
                     AVLAHEIGHYKKKHTRTMIVASLLQMGLLLYIFGLFVNSPWLTEALGIPYEQGKFHIA
                     LFVFSILYSPLSFIISIGMNILSRKHEFEADAFASQQYSGEYLEQSLRKLSKRNLSNL
                     TPHPLYVFFYYSHPPLLQRIRAMRSIKK"
     gene            1750..>1946
                     /locus_tag="P5243_08160"
     CDS             1750..>1946
                     /locus_tag="P5243_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRS19463.1"
                     /translation="MKRSNFQTLSQVIDDFFRKNGMEEKIMEERIKDSWNLVVGPIFA
                     KATQQIKISNGVLIVTMKSAAV"
BASE COUNT          699 a          252 c          341 g          654 t
ORIGIN      
        1 aaaaaaagtg gtttcaaagg aattgccggt atttctgata tgcgtgatat gaaacaggcc
       61 gcacaacaag gaaatgaacg agctcagtta gctcttgata tgcttgcata tagaattaaa
      121 aaatatatcg gggcttatgc agctgctatg caaggagttg attgtgtagt gtttaccggt
      181 ggtataggtg aaaataatcc cgatttgcga aaacaggtgt gtagcggatt agaatttatg
      241 ggagtgtatt ttgatgaaca agcaaatacc aacagtcaat cacatgaata tattatttct
      301 caaccatctt cggctattac tgtaatggtg attcaaacca acgaagaaat agttattgca
      361 cgcgatacat atgaattaat tacacataat taacatgtga ttattttatt gtaaatacaa
      421 gaataaatat tcacatatat tctatacata aaagcatata tttgcaaaaa aataaatagt
      481 atggcatcaa caatttttgg aataataata gcagttttag tatttgatta tgctcttgaa
      541 cggattttag atttattaaa tgcaaaacat agagttcaag aattaccaat agaattgcaa
      601 gatatatata atgccgaaga atatgccaaa cagcaacaat atgaacaaac aaaaacacaa
      661 tttggattca ttacagcatc atttagtttt atactaattc ttggaatgtt attgtttgac
      721 ggatttgctg taattgatac atatatctat tcttcatttg gtactcccca agagccatta
      781 aatcatattt tgggtggatt gttgtttttt ggtattgtta tgtttgcgtc agatatattg
      841 ggaataccat tcgaaatcta ttctacattt gtaatagaag aaaaatttgg atttaataaa
      901 actacaccaa aaatatttgt ttttgataaa ttaaaatcat ggttgctttc catattacta
      961 gggggcggaa tattggcagc actattattt ctaattagtt attttggtaa ttatttttgg
     1021 gtatacgcat ggatatttgt tgctcttatc atgattgcta tcactatgtt ttattctaca
     1081 cttatagttc ccttgtttaa caagcaaaca ccattagaag aaggtgaatt aaaacaagca
     1141 atctttgaat ttagttcaaa agtaggattt aaacttgata atatttttgt tatagacggt
     1201 tcaaaacgtt caactaaagc taatgcttat tttagtggat taggagcaaa aaaacgaatt
     1261 gtattgtatg atacacttat acaagaaatg acaacagaag aaatagttgc tgtattggca
     1321 catgaaatcg gacattataa gaaaaaacat acacgaacaa tgatagttgc atcgttgtta
     1381 caaatgggat tattgttata tatattcgga ttatttgtaa acagtccttg gttaacagag
     1441 gcattgggaa taccttatga gcagggaaaa tttcatatag cattatttgt ttttagtatt
     1501 ttgtattcac cattgtcttt tataataagt attggaatga atatattgtc gcgtaaacat
     1561 gaatttgagg ctgatgcatt tgcttcacaa caatatagtg gtgaatattt agaacaatca
     1621 ttacgtaaat tatcaaaacg aaatttaagt aatcttactc ctcatccgtt gtatgtattc
     1681 ttttattatt cgcatccgcc attgttacaa agaattagag caatgcgttc aattaaaaaa
     1741 tagaaaacaa tgaaacgaag taattttcag accttgtcgc aggtaattga tgattttttt
     1801 agaaaaaacg gcatggaaga aaaaattatg gaagagcgaa taaaggattc ttggaattta
     1861 gttgtaggac caatatttgc aaaagcaacg cagcaaataa aaatttcaaa tggggtatta
     1921 atagtaacaa tgaaatcggc tgcagt
//
LOCUS       DAOTOW010000461         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45797_length_1942_cov_2.298357, whole genome shotgun sequence.
ACCESSION   DAOTOW010000461 DAOTOW010000000
VERSION     DAOTOW010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45797_length_1942_cov_2.298357"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="P5243_08165"
     CDS             complement(<1..669)
                     /locus_tag="P5243_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRS19464.1"
                     /translation="MYQANDLKKMLVSDLKEIATQLGVQNPGSLKKEDLIIRILEEQG
                     SIPRNLPIEEDNEVKQGELPFESSHPEEEGEKRQRKRTRTIVQVSKPEKVQPRSNTVE
                     IKEEVVASISSEEDTIIAAAPILTEPETHTEIIENQEPIALNEEKQQNHKQHHQNQQN
                     NQQQRQEKQEKVTEFDGIVIGSGVLDMMQEGYGFLRSGDYNYLNSPDDIYVSQSQIKH
                     FGLKT"
     gene            complement(844..>1942)
                     /locus_tag="P5243_08170"
     CDS             complement(844..>1942)
                     /locus_tag="P5243_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS19465.1"
                     /translation="NIGSPVNTRNWESQPSLSADGRTLYFVSNRPGGYGKKDIWVSTL
                     TDTNTWTPPVNLGPTINTKEEEESPYIHADGKTLFFSSDGHFGMGKKDMFVTQKLANG
                     SWRQPINMGYPLNTHNKESHVVVNAAGTKGYFASNQFGTLGGVDIFEFDIDETFPIKP
                     SPVTYIKGVVYDAKNIDKKVPALIQLSDIETGEVFYEHVADFYTGSFIVPFYQDKSYA
                     LTVTHHGYLLYSEQVNLKTIGKSISIPMQPIAIGNTVVLNNVFFDTDSYSLKPESKVE
                     LLAIVDFMNQNPLICFEIGGHTDNVGNKQYNTTLSNNRAKAVYDFIVEAGISSSRLSY
                     KGYADNKPVVDNTTPENRAKNRRTELVITKF"
BASE COUNT          596 a          327 c          284 g          735 t
ORIGIN      
        1 ggttttcagt ccgaaatgtt ttatctgcga ctgagaaaca taaatatcat cgggtgaatt
       61 taaataatta taatcgcccg aacgtaaaaa tccatagcct tcttgcatca tatcaagtac
      121 cccagaacca ataactattc cgtcaaattc agtaactttt tcctgttttt cttgtcgttg
      181 ttgttgattg ttttgctgat tttgatgatg ctgtttatga ttttgttgtt tttcttcatt
      241 tagagctatt ggttcttgat tctcaataat ttctgtatgc gtttcaggtt ctgtcaaaat
      301 tggcgcagct gcaattattg tatcttcttc tgatgaaatt gaagcaacaa cttcttcttt
      361 tatttcaaca gtatttgaac gtggttgaac tttttcgggt ttacttacct gtactattgt
      421 acgagtacgt ttacgttgac gcttctctcc ttcttcttcc gggtgcgatg actcaaaagg
      481 taattcacct tgttttactt cattatcttc ttctataggc aaattacgag gaatacttcc
      541 ttgttcttct aaaatacgta tgattaaatc ttcttttttt aaagagccgg gattttgcac
      601 tcctaattgt gttgctattt cttttaaatc agaaacaagc atttttttta aatcgtttgc
      661 ttgatacata aaaaaagtat gtgaattaca aattgaataa ttatttgata tttttttggg
      721 atttttagat ttttatcggc acatatctat tgtttcaccg aaatccaatg caataataca
      781 tattatattt taaaaaataa caattacgtg taaaaaaaaa tataattgca tacatttacg
      841 caattaaaat ttagtaatta ccaactctgt acgtctgttt tttgctctat tttcaggcgt
      901 tgtattatcc actacgggtt tattgtctgc atatccttta tatgataacc gtgaagatga
      961 aattccagct tctacaataa aatcgtatac cgctttagct ctattattag aaagagttgt
     1021 attatattgt ttattcccta cattatcggt atgacctcct atttcaaaac aaattaaagg
     1081 attttgattc ataaaatcaa ctattgccaa taattccact tttgattctg gttttaaaga
     1141 atatgaatca gtatcaaaaa acacattatt taaaactaca gtattaccaa ttgctatagg
     1201 ttgcatagga atactaattg atttaccaat agtttttaaa tttacttgtt cggaatataa
     1261 caaataacca tgatgtgtaa cagtcaacgc atatgattta tcttgataaa aaggtactat
     1321 aaaactaccg gtataaaaat cagctacatg ttcataaaaa acttcacctg tttctatatc
     1381 acttaattga ataagagcag gtactttttt atcaatattt ttagcatcgt ataccactcc
     1441 tttaatatat gttacaggag atggtttaat aggaaaagtt tcatctatat caaattcaaa
     1501 tatatcaaca ccaccaagag ttccaaactg atttgatgca aaatagcctt tagtacctgc
     1561 tgcgtttaca actacatgtg attctttatt atgagtattg agtggatatc ccatatttat
     1621 aggttgtcgc caagaaccat tagcaagttt ttgagtaaca aacatatcct ttttgcccat
     1681 accgaaatgt ccatctgatg aaaaaaacaa tgttttacca tcggcatgaa tatatggtga
     1741 ttcttcttct tcttttgtat ttatagttgg gcctaaattt accggtgggg tccatgtatt
     1801 tgtgtcggta agcgtactta cccatatatc ttttttacca tatccaccgg gtctatttga
     1861 aacaaaatat aatgtacggc catcggcaga taaacttggt tgagattccc aatttcgagt
     1921 atttaccgga gaaccaatat tt
//
LOCUS       DAOTOW010000462         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_45957_length_1937_cov_2.277896, whole genome shotgun sequence.
ACCESSION   DAOTOW010000462 DAOTOW010000000
VERSION     DAOTOW010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45957_length_1937_cov_2.277896"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="P5243_08175"
     CDS             <1..755
                     /locus_tag="P5243_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19466.1"
                     /translation="EFESGGVYSSASYNSSTVIGGSGFHPRNATNAPDAAGGNYLSYK
                     GGQITSASVISNLAAGADVLNVKPEAWELPITLLSFIAQKVQGGVLLEWVTASEKNND
                     YFTVYRSHNGFDFEAIGSMSGAGTTNISHEYSYTDYSVFGGVTYYKLGQTDYDGKKTY
                     SPILSVFEDTPTFEILRCINTVSTQCTLYIQLNTADINNLYIINSNGAIVYNKIFVNT
                     VYEQIDITLTPGIYTVINKTKNSVNSVQILVK"
     gene            complement(913..>1937)
                     /gene="rlmD"
                     /locus_tag="P5243_08180"
     CDS             complement(913..>1937)
                     /gene="rlmD"
                     /locus_tag="P5243_08180"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRS19467.1"
                     /translation="YRNKLEFTFSNKRWITQEEIIHNTAITQSNAVGFHVPKLFDKIV
                     DIETCYLQAEPSNAIRNFIREFALIHNYSFFDIRNHTGLLRTLIIRTTSKGEIMLIVV
                     FYENDAHKHKHILDAIYKEFPQITSLLYCINQKANDTIHDQEILVFAGNDHIVEYMED
                     LQFKIGPKSFYQTNSNQAYKLYCITREFAQLQGHEIVYDLYTGTGTIANFIAKHARKV
                     VGIEYVPEAIEDAKQNSLQNAITNTSFFAGDMKDILTDEFIQIHGSPDVIITDPPRAG
                     MHQDVVDTIIRCKPQRIVYVSCNPATQARDLQLLSSIYTIEKSQAVDMFPHTHHVENV
                     VLLTKK"
BASE COUNT          634 a          279 c          350 g          674 t
ORIGIN      
        1 cggaatttga gtcaggtggt gtatattcgt cggcttctta taattcgagt actgttatag
       61 gcggttccgg tttccatccg cgtaatgcaa caaacgctcc cgatgctgcc ggaggaaatt
      121 atttatcata taaaggagga caaattacta gtgcttcagt gatttcaaat ttagcggcag
      181 gagcagatgt actgaatgta aagcccgaag cttgggagct tcctattaca ttattgtctt
      241 ttattgctca aaaagttcaa ggaggagtgt tacttgaatg ggtaacagca agcgaaaaaa
      301 acaatgatta ttttaccgta tatcgttcac ataatggatt tgattttgaa gcaatcggaa
      361 gcatgtcggg ggcaggtaca accaatattt cacatgaata cagttatacc gattatagtg
      421 tatttggagg agttacgtat tataaattag gtcaaacaga ttatgacggt aaaaaaacat
      481 attcgccaat actatcggta tttgaagata cacccacatt tgaaattctt cgttgtataa
      541 atactgtttc aactcaatgc acattatata ttcaattgaa tactgctgac ataaataatt
      601 tgtatataat aaattcgaat ggtgctattg tgtataataa aatatttgtg aatacagtat
      661 atgaacaaat agatataaca ctaactccgg gaatatatac cgtaattaat aaaacaaaaa
      721 attcagtaaa ttctgttcaa atattagtaa aataaaaaag cagactccgt aaaggacgtc
      781 tgctaaaatg aggtggtaag gtaagtcaat agtttccgat acaaatatag aatttttttg
      841 tttataaacg caataaaagt gtgtaaaaat accattattt tttatgctgt tttatatgta
      901 attgactatc agttattttt ttgtaagtaa aacgacattt tcaacatgat gagtgtgagg
      961 aaacatgtct actgcttgtg atttttctat ggtatatatt gatgatagta gttgtaaatc
     1021 gcgagcttgt gttgccggat tacaacttac atatacaatt cgttgcggtt tacatcgtat
     1081 aattgtgtca actacatctt gatgcatacc tgcacgaggc gggtctgtta ttataacatc
     1141 gggagatccg tgaatttgaa taaattcatc ggttaatata tctttcatgt cgccggcaaa
     1201 gaaagatgta tttgtgatgg cattttgtaa cgaattttgt ttggcatctt ctattgcttc
     1261 cggtacatat tcaattccaa caactttgcg agcgtgtttt gcaataaaat tagcaatagt
     1321 gcctgttcct gtgtataaat catacacaat ttcatgtccc tgcaattgag caaattctcg
     1381 tgttatacag taaagtttat atgcttgatt ggaatttgtt tgataaaatg atttgggacc
     1441 aattttaaat tgtaaatctt ccatatattc tacaatatgg tcatttccgg caaatactaa
     1501 tatttcttga tcgtgaatag tgtcgttagc tttttggttt atacaataaa gaagagacgt
     1561 aatttgtgga aattctttat aaatagcatc taatatgtgt ttgtgtttat gagcatcatt
     1621 ttcataaaat actacaatga gcatgatttc tcctttactt gtagttctta taatgagtgt
     1681 acgaagtaat cctgtgtgat tgcgaatatc aaaaaagcta taattatgaa taagagcaaa
     1741 ttcacggata aaattacgaa tagcattact tggttcagct tgtaaataac aggtttctat
     1801 atcaactatt ttgtcaaaca attttggaac atgaaaacca accgcatttg attgagtaat
     1861 tgcagtgtta tgtataattt cttcttgtgt aatccatcgc ttgtttgaaa acgtaaattc
     1921 taatttattg cgatata
//
LOCUS       DAOTOW010000463         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_46068_length_1934_cov_2.138904, whole genome shotgun sequence.
ACCESSION   DAOTOW010000463 DAOTOW010000000
VERSION     DAOTOW010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46068_length_1934_cov_2.138904"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1295
                     /gene="nhaA"
                     /locus_tag="P5243_08185"
     CDS             90..1295
                     /gene="nhaA"
                     /locus_tag="P5243_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRS19468.1"
                     /translation="MELTKIYNDFFNSEKAGGLVLIICTVFSLWIANSFLGPHYQAIW
                     NFELAGHSIVHWINDGLMAIFFLLIGLELEREIYKGELSNIREALLPIFSAIGGIIVP
                     ALLFLFLNYAEPTRSGFGIPMATDIAFAIGILSLLGNKIPLSLKIFLTALAVIDDLGA
                     ILIIVFFYSKGIAWTYLLIAIGIYVVLIVLNRLRVTQYMPYIIGGIAMWYCMLHSGIH
                     ATITGVLLAFAIPYGNGCSQCPSTKIQHMLHKPVAFIILPIFALANTAIIINSKLGDI
                     LFEPYSLGILLGLILGKPIGIFVLTYIAVTFKISKLPENVDWKMIAGVGMIAGIGFTM
                     SIFITLLAFSDEAIINNAKFAILMASLVSGISGYVFLRMQSRKIGFQNQKAKKHTDDH
                     NCTCGKYSK"
     gene            1331..>1934
                     /locus_tag="P5243_08190"
     CDS             1331..>1934
                     /locus_tag="P5243_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HRS19469.1"
                     /translation="MYSVLIVLFTTILFQSQLFSQITPFDVLPHIMRGINLGNTFETD
                     SVEGHWKQDAPEEWKDNGPAQEYYFDAYKQAGFTCVRIPVTWHTRTLYESPYTIDNNW
                     LQRVDEVVEWALERNLFVILNAHHETWLKENYSNAQYRERFDSIWSQVSMYFKDKPER
                     LMFEIINEPLGMSVSEVDDVNARILQIIRKSNPTRIVLFSG"
BASE COUNT          597 a          257 c          345 g          735 t
ORIGIN      
        1 ataattgaaa aatcagaact tcgattttgc gtgttttata caaaaaacaa aaagtttttt
       61 agtattttgc ctacaaaatt tctaatacaa tggaattaac taaaatatat aatgatttct
      121 ttaatagtga aaaggctgga ggtcttgtac ttataatttg tacagtattt tcattatgga
      181 ttgctaattc atttttggga cctcattacc aagctatatg gaactttgaa cttgccggtc
      241 attcaatagt tcattggatt aatgatggct taatggctat tttttttctt cttattggtc
      301 ttgaattaga acgcgagatt tataaaggtg aattatcaaa tattcgagaa gcgttattgc
      361 ctatatttag tgctattggt ggaattattg tacctgcatt attatttttg tttcttaatt
      421 atgctgaacc tactcgttca ggatttggaa tacctatggc aaccgatata gcatttgcta
      481 ttggtatatt atccttatta ggaaataaga ttcccttatc attaaaaatt tttttaacag
      541 ctttagcggt aatagatgat ttaggagcaa tactaattat tgtatttttt tattcaaaag
      601 gaatagcttg gacatatttg ttaatagcaa ttggtattta tgtagttctt attgttctta
      661 acagattgcg ggtaactcag tatatgccat atataattgg aggtatagct atgtggtatt
      721 gtatgttgca ttccggtatt catgctacaa ttaccggtgt tttgcttgct tttgcaatac
      781 cgtatggaaa tggttgcagt caatgtccgt caactaaaat tcaacatatg cttcataaac
      841 ccgttgcgtt tattattttg ccaatttttg ctctcgctaa tactgctatt attattaatt
      901 ctaaacttgg tgatattttg tttgaacctt atagtttagg cattttacta ggattaattt
      961 taggaaaacc aattggaatt tttgtgctta catacattgc tgtaacgttt aagatttcta
     1021 aattgcctga aaatgttgat tggaagatga ttgccggtgt gggcatgatt gccggtatag
     1081 gatttaccat gtcaattttt attactcttt tggcattttc agatgaagct attattaata
     1141 atgcaaaatt tgcaattctt atggcttctc ttgtctccgg tatttctgga tatgtatttt
     1201 tgcgaatgca atcacgtaaa atcggatttc aaaaccaaaa ggcaaaaaaa catactgatg
     1261 accataattg tacctgtgga aaatatagta aataatcttt ttgtgtgtcg aaatgaagtt
     1321 atataaaata ttgtattctg ttttaattgt attatttact acaattttgt ttcagtctca
     1381 attattttct caaattacac cgtttgatgt attgcctcat ataatgcgag gaattaattt
     1441 gggtaatacg ttcgaaacag atagtgttga aggacattgg aaacaagatg ctcctgaaga
     1501 atggaaagat aatggacctg cacaagaata ttattttgat gcatataaac aggctggttt
     1561 tacatgtgtt cgcatacctg taacttggca tacgcgtacc ttatatgaat ctccctatac
     1621 tattgataat aattggttac agcgtgttga tgaggttgta gaatgggctc ttgaaagaaa
     1681 tctttttgtt atacttaatg ctcatcatga aacatggctc aaagaaaatt attcaaatgc
     1741 tcaatataga gaaagatttg atagtatttg gagtcaggtg tcaatgtatt ttaaagataa
     1801 accggaaagg cttatgtttg aaattattaa tgaaccattg ggaatgtcgg tttctgaagt
     1861 tgatgatgta aatgctcgga ttttacaaat tataagaaag tcaaatccta caagaattgt
     1921 tttattttca ggca
//
LOCUS       DAOTOW010000464         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_46177_length_1930_cov_3.174933, whole genome shotgun sequence.
ACCESSION   DAOTOW010000464 DAOTOW010000000
VERSION     DAOTOW010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46177_length_1930_cov_3.174933"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1111)
                     /locus_tag="P5243_08195"
     CDS             complement(<1..>1111)
                     /locus_tag="P5243_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19470.1"
                     /translation="ANIDADFEAFKFTLKDNTMSLNDLDLEYNGWLAMPGDDIEMDFT
                     CKTLKTDFKNILSLVPGVYMEGFENIKTSGKLALEASLKGVYSEKPETYPSFNIGLLV
                     ENGMFQYPDLPKSVDNINIDLRVSNPSNNLDSTVVDLKKFSMKFAQNPFSLSFFMKTP
                     MSDPFMKADVSGTIDLESMKDFIPLDSTTIKGVIQPQLQFAAKLSDVEKENYTAITAL
                     GKLVVTNFYYKDADVPTGVAVSNMTMNFSPQFVDMQQCNIVMGKSDMSLTGKLENVIA
                     YVMSNQTIKGTLTLKSQYFDANEIMGEDSSSAPDTTTSDEPYEIIEVPGNIDFVLNST
                     FGRLLYDTYDVTNVVGKITIKDRKVSMDKLSMNLLG"
     assembly_gap    1112..1121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1122..1770)
                     /locus_tag="P5243_08200"
     CDS             complement(<1122..1770)
                     /locus_tag="P5243_08200"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRS19471.1"
                     /translation="MKKALKIIGIVLVVLLLALVIAPYAFRGKLMELTKQEINKSLTA
                     KVDFKGFGVSILKNFPDLTVTIKQLEIVGTEQFSNDTLIAVEKLLATVDIKSVISGDS
                     IGVKQIEIIKPRIHALIAADSSANYDIVKSSDTDTVSEVDTSTTKFSMQLDKISIIDA
                     FIYYGDIPGNMSATLDGMNMELQGNFTETTTLIKTVISVAKLSYTMDGIPYLKNVN"
BASE COUNT          666 a          337 c          244 g          673 t
ORIGIN      
        1 cccaacaaat tcattgaaag tttatccata ctaaccttac gatctttaat tgtaattttt
       61 ccaacaacat ttgttacatc atatgtatca tacaacaatc gtccaaacgt tgagtttaac
      121 acaaaatcaa tatttccggg gacttcaata atttcataag gttcatcgct ggttgttgta
      181 tcaggagcag aacttgaatc ttcgcccata atttcgtttg catcaaaata ttgtgacttc
      241 aatgttaaag tacctttaat agtttgattt gacatgacat atgctattac attttcaagt
      301 tttccggtca atgacatatc acttttaccc attacaatat tacattgctg catatcaaca
      361 aattgcggag aaaaattcat agtcatatta gaaacggcaa caccagttgg cacatctgcg
      421 tctttataat aaaaatttgt aacaacaagt tttccaagtg cagtaatagc agtataattt
      481 tctttttcaa catcagaaag tttagccgca aattgtaatt gtggttgtat tactcctttt
      541 atagttgtag aatcaagagg tataaaatct ttcatggatt ccaaatcaat agtacccgaa
      601 acatctgctt tcataaatgg gtcagacata ggagttttca tgaaaaatga tagagaaaat
      661 ggattctgag caaatttcat agagaatttc tttaaatcta caacggtact atccaaatta
      721 tttgaaggat ttgaaactcg taaatctata ttaatattat caacagattt gggcaaatcg
      781 ggatattgaa acataccatt ttctaccaac aaaccaatat taaatgacgg atatgtttcg
      841 ggtttttcac tatacacccc ttttaatgaa gcttcaagag ccaattttcc ggaagtttta
      901 atattttcaa atccttccat atataccccc ggcacaagtg ataaaatatt tttaaaatca
      961 gttttcaatg ttttgcacgt aaaatccatc tcaatatcat caccgggcat agcaagccat
     1021 ccgttatatt ccaaatccaa atcatttaaa ctcattgtat tatctttaag tgtgaattta
     1081 aatgcctcaa aatcggcatc aatattggca tnnnnnnnnn naattcacat tttttaaata
     1141 tggtatacca tccatagtat acgatagttt tgcaacagat ataacagttt taataagagt
     1201 tgtagtttcg gtaaaattac cttgcaattc catattcatt ccatcaagag tagccgacat
     1261 attacccgga atatctccat aataaataaa tgcatctatt atagatattt tgtctaattg
     1321 catagaaaat tttgtggtac ttgtatcaac ttctgaaacc gtatcagtat cgctactttt
     1381 tacaatatca taatttgcac tgctgtctgc tgcaattagt gcatgaattc gaggttttat
     1441 aatttctatt tgttttacac caattgaatc accagaaata acactcttaa tatcaactgt
     1501 agcaagaagt ttttcaacag caataagagt atcattagaa aattgttcag ttcctactat
     1561 ttctaattgt ttaattgtaa ctgttaaatc aggaaaattc tttaaaatac ttactccaaa
     1621 tcctttaaaa tcaaccttag ccgtaagtga tttattaatt tcctgttttg taagttccat
     1681 taatttacca cgaaatgcat atggagcaat cacaagtgct aacaacaaca ctaccaacac
     1741 aataccaata atttttaacg cttttttcat tgcagaaatg ttttaattag ttttataaaa
     1801 taaaagtaca ttattttttt taatcacaca ttataccaaa taattttata cacttttatt
     1861 atttcttatt tattaaatat ttgaagcaac acaggaaaat ggtcactata accacctgta
     1921 aaataagctc
//
LOCUS       DAOTOW010000465         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_46377_length_1924_cov_3.012306, whole genome shotgun sequence.
ACCESSION   DAOTOW010000465 DAOTOW010000000
VERSION     DAOTOW010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46377_length_1924_cov_3.012306"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="P5243_08205"
     CDS             <1..636
                     /locus_tag="P5243_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19472.1"
                     /translation="SRFGFWERYVGPYNRIHNNYRRSFETISITDTVVDYKKSFVSFD
                     LELKYKTIVLNRPVIFVNYTQFSSVHDKLTLLPRAYPVTILTKEHSLPDTNTFFQLFY
                     NEFTVFYNVQRRVTLVGLFGYEAALANMRTELADTNGNLITDANGRIIYSSNGKPMHQ
                     VDFGYGIGVDYNFHDRASLHVRQRWYNHKDYNFTKDKFAGNELTVELKMFF"
     gene            662..>1924
                     /locus_tag="P5243_08210"
     CDS             662..>1924
                     /locus_tag="P5243_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19473.1"
                     /translation="MKKKVLLISSITVLMNIYVHAQNTYFTGHGRSLFTYDDMTDEVQ
                     ADSKQAASSGYTMYDFGVHAENKGLVRASAILRIRNEFGGFYADGASMDIRQVQIEGV
                     IQNRVKFEVGDMYLSQTPYTIWNFDESFIKYESDIFGIRRKIVNYENYFIDNKWRLQG
                     ANAYTTLQFMKGIKSLTVQGYGSRILPSNLLEIPDRFLYGTKLSIIQSNAFKLSGNYV
                     GISDLTGTVPAANVDFENNVYTGDFKYSYDKIAGYSFALLGETGFSNTSLREDAFNIN
                     KTKHDYFYDAGVQLEASKKHTSIFTFKYRDVGPYFTSPAAQTRRIIENAADYSLEVFP
                     NFSDGTSLRSMTLLDRMSQETGLYNGSISPLLLPYLPQYNSAEPYGQATPNRSGATVA
                     LGFADSARIVAVNAEANMLSEIFAEGDTT"
BASE COUNT          614 a          326 c          388 g          596 t
ORIGIN      
        1 tcgcgttttg gattttggga acgctatgtt ggtccataca acagaattca caacaattat
       61 cgtcgttctt tcgaaactat ttcaattacc gacacggttg tagattataa aaaatcattc
      121 gtaagttttg atttggaatt gaagtacaaa actattgtgc tcaatcgtcc tgtaattttt
      181 gtgaattata cacagttcag ttcggtacac gataaactta cattgttgcc tcgggcgtat
      241 ccggttacta ttcttaccaa agaacattct ctgcccgata caaatacatt ttttcagttg
      301 ttttacaatg aatttacggt attctacaat gtgcaacgac gtgtaactct tgtgggattg
      361 tttggctacg aagctgcact tgcaaatatg cgtaccgaat tggccgatac caatggcaat
      421 ttaattaccg atgccaatgg acgcattata tatagcagca acggaaaacc tatgcatcag
      481 gttgactttg gctatggaat aggcgtcgat tataattttc acgatagagc aagtttgcat
      541 gtgcgtcaac gatggtacaa tcacaaagat tacaacttta ccaaagataa atttgccgga
      601 aacgaattga cggtagaatt aaaaatgttt ttttagtacg aatttacaat aagatatata
      661 tatgaaaaag aaagttttac ttataagttc tattaccgtg ttgatgaata tatatgttca
      721 tgcacaaaac acgtatttta ccggacatgg caggtcgttg tttacctacg acgatatgac
      781 cgatgaagtt caagccgata gtaaacaagc ggcttcgtcg ggatatacca tgtacgattt
      841 tggtgtacat gccgaaaaca aaggtttggt gcgtgcaagt gccatacttc ggattcgtaa
      901 tgaatttggc gggttctatg ccgatggagc atcaatggat atacgtcaag tgcaaataga
      961 aggagttatt caaaatagag taaaattcga agtaggagat atgtacctga gtcaaactcc
     1021 ctatactatt tggaatttcg atgaaagttt tatcaaatac gaaagtgata tttttggaat
     1081 tcgcagaaaa attgtgaatt acgaaaatta cttcatagat aataagtggc gtctgcaagg
     1141 tgcaaatgca tatactacgc tccaatttat gaaaggaatt aagtcgttga cagtgcaagg
     1201 atacggttcg cgaatattac cttcaaattt attggaaata cccgatagat ttttgtatgg
     1261 aacaaaactt agtattatac aaagtaatgc tttcaaactt tcaggaaatt atgttggaat
     1321 aagcgatttg accggaacgg tgcctgcagc taatgttgat tttgaaaata acgtgtatac
     1381 cggcgatttt aagtattcgt atgataaaat tgcaggatat tcatttgcat tgttgggcga
     1441 aacgggtttt tcaaacacat cgttgcgcga agatgctttt aatataaata aaacaaaaca
     1501 cgattatttt tacgatgccg gtgtgcaatt agaggcttct aaaaaacaca catctatttt
     1561 tacatttaag tatcgtgatg taggaccata ttttacaagt cctgcagcgc aaactcgcag
     1621 aattattgaa aatgcagcag attattcgtt agaagtattt ccaaatttta gtgatggtac
     1681 atcattgcga agtatgactt tgctcgatag aatgtcgcaa gaaaccggtt tgtacaatgg
     1741 tagtatatcg cctcttttgt tgccatacct gccacaatac aatagtgccg aaccatacgg
     1801 acaagctaca ccaaaccgtt cgggagctac tgttgcatta ggatttgccg attctgctcg
     1861 tatagtagct gtaaatgcag aagctaatat gctctcggaa atctttgccg aaggtgatac
     1921 taca
//
LOCUS       DAOTOW010000466         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_46427_length_1923_cov_2.623662, whole genome shotgun sequence.
ACCESSION   DAOTOW010000466 DAOTOW010000000
VERSION     DAOTOW010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46427_length_1923_cov_2.623662"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..567
                     /locus_tag="P5243_08215"
     CDS             280..567
                     /locus_tag="P5243_08215"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19474.1"
                     /translation="MFGNGCVVSLRDSYIPRRTYVQRIIDIIRRSQLPFQCEVEKSGG
                     SDGGYLQVTPYAVDWCFVGAPQEFSHTPHEKIYKDDLYSMLLIYKVLMKEL"
     gene            661..>1923
                     /locus_tag="P5243_08220"
     CDS             661..>1923
                     /locus_tag="P5243_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRS19475.1"
                     /translation="MKHIAKLVFFLIFIIVDYGFLNPGEPPQTYVPLTKNPFEHVNKE
                     WVDSVYNSMTLEQRIGQLFMVAAYTTPDQSNKQQIAELITNYGIGGIIFMKGTPINQV
                     LYTNYFQSLSNVPLLVAIDGEWGLAMRLDSTISFPRQLMLGAVTDNKLIYEMGYEIGR
                     QCKRMGIHVNFAPVVDINNNPNNPVINSRSFGELKHNVAVKGDYYMTGMQDQGIITTA
                     KHFPGHGDTDADSHKSLPIIPHTKARLDSVELYPFNYLIQRNLTGVMVAHLYIPEIDR
                     TKNQASTLSPKIVQDMLRNQLGFNGLIFTDALNMQGVSKFYKPGLLEIKALLAGNDVL
                     LFPENVPVAVAAIKEAIKTGKMSEKIIENRCKKILAAKSWAGITDSIAPISTAHLYED
                     LHTPQAELLVKKLVRASITVAKDDANQLP"
BASE COUNT          691 a          273 c          340 g          619 t
ORIGIN      
        1 aaaaacacaa aaaaataaaa aaggaaatag atttttaaac aaccaataac attttgaaat
       61 tataagaaat gaattctaaa gtttctaacc atcatttttc ttttgtttat ccattattac
      121 acatgattag gggctaaaat gagtgtctat taatcaagtt ccaatcgaaa tttaaatgga
      181 atagcagtag caataataaa caaaaatgtt attttatata aaaaatatca tattcgtcag
      241 gcattaattg ctgatattac atgggtaacc aaaggaattt tgtttggtaa cggttgtgta
      301 gtatctttgc gtgattctta tattccgcga agaacctatg tgcagcgaat aattgatatt
      361 attcgccgtt cgcagttgcc atttcaatgt gaagttgaaa aaagtggtgg tagcgatggc
      421 ggttatcttc aagtaacacc atatgctgta gattggtgtt ttgttggtgc cccacaagaa
      481 ttttctcata cacctcacga aaaaatatat aaagacgatt tgtattctat gttgttgatt
      541 tataaagtgc taatgaaaga gttgtaaaat tgaatacttt aaaaagaaag atgaatattt
      601 atgatagtat ttttgaataa taaatgcaaa ataattgaaa caaaacatgt attgaaaacc
      661 atgaaacata tagcaaagct tgtatttttt ttaattttta taatagttga ttatggtttt
      721 ttaaatccgg gagaacctcc acaaacatat gtgcctctta caaaaaatcc gtttgaacat
      781 gtaaataaag aatgggtgga ttctgtgtat aattctatga ctttggaaca aagaattggt
      841 caattgttta tggtagcggc atatacaaca cctgatcaaa gcaacaaaca acaaatagca
      901 gaattaatca ctaattatgg tattggtgga attattttca tgaaaggtac tcctataaat
      961 caagttctat atactaatta ttttcaatcg ctaagtaatg tcccattatt ggttgcaata
     1021 gatggtgaat ggggattggc aatgcgattg gatagtacaa tttcatttcc ccgacaatta
     1081 atgctgggtg ctgtaaccga caataaattg atttatgaaa tgggatacga aattggacgt
     1141 caatgcaagc gtatgggaat tcatgtgaat tttgcgcctg ttgttgatat taacaataat
     1201 ccaaataatc cggtaataaa ttcacgttct tttggagaat tgaaacacaa tgttgctgta
     1261 aaaggtgatt attatatgac cggtatgcaa gatcaaggta ttataacaac tgcaaaacat
     1321 tttccaggac atggtgatac cgacgctgat tctcataaaa gccttccaat aattccacat
     1381 accaaagctc gacttgacag tgttgagttg tatcctttta attatcttat tcaacggaat
     1441 ttaaccggtg ttatggttgc acatttatat attcctgaaa ttgacagaac aaaaaatcag
     1501 gcatctacat tatctcctaa aattgtgcaa gatatgttac gaaatcaatt gggatttaat
     1561 gggttaatat ttaccgatgc tcttaatatg cagggagtta gtaaatttta taaaccggga
     1621 ttacttgaaa ttaaagcact tttggcaggg aatgatgtac tattatttcc tgaaaatgtg
     1681 cctgtagctg tagcagcaat taaagaggca ataaaaaccg gaaaaatgag tgaaaaaata
     1741 attgaaaata gatgtaaaaa aatacttgca gcaaaatcat gggcgggtat aaccgattct
     1801 attgctccta tttcaactgc tcatttatat gaagatttac ataccccgca agccgaatta
     1861 ttggtgaaaa aattagtaag agcttcaatt acagttgcta aagacgatgc gaatcaatta
     1921 cct
//
LOCUS       DAOTOW010000467         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_46920_length_1908_cov_3.166217, whole genome shotgun sequence.
ACCESSION   DAOTOW010000467 DAOTOW010000000
VERSION     DAOTOW010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46920_length_1908_cov_3.166217"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1908
                     /gene="priA"
                     /locus_tag="P5243_08225"
     CDS             <1..>1908
                     /gene="priA"
                     /locus_tag="P5243_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRS19476.1"
                     /translation="LPLAVPLLYTYAIPHELASQVQVGTRVLVQLGTRKIYTAIVYSI
                     HLNKPQHYQVKCIDSVLDSIPIVTPIQLQLWDWIAKYYMCTRGEVMKAALPAGLKLES
                     EALLYCNPIFDSIDTLSNNELIVYSAVQNAQGISLDELTKIVKKIPVTRTVHGMLQNK
                     VLLTGEKVIQQYKPKTETRIQLAGSISDETFVNTSFEMVAKAPKQLELFTKFLDLHIK
                     SKNPDFSILKKELLLVTGCSASIVAELTKKNILVECNTNVSRLHNTHVELQEFKILSE
                     SQQKALQQINTAFKTHDTCLLHGVTASGKTEVYIELIKQTIAQNKQVLYLLPEIALTS
                     QIIDRLQKVFGNIVGVYHSRFSDNERIEVWNNVLSLSTYKIILGVRSSIFLPYSNLGL
                     IIVDEEHETSLKQFDPAPRYNARDTAIILAQLHKAKTLLGTATPSLETYYNVLQKKYA
                     LVELNQRFSQVQLPQIHIIDMAKARKQKRMYNAIFSKDLLNQIELALQEKQQVILFQN
                     RRGFTPLIQCTDCGNIPHCKHCDVSLTYHKYSNTLRCHYCGYAIQYSPNCIACGSNRQ
                     EYLGFGTEQIQDTLQEIFPHKHIARMDLDTTRGKYAYHELIEQFEHREIDILIGTQMI
                     TKGLDFENVGLV"
BASE COUNT          696 a          295 c          312 g          605 t
ORIGIN      
        1 acttccgttg gctgttcctc ttttatatac ctatgctata ccacatgaat tagcaagcca
       61 agttcaagta ggaacacgtg tattggtgca attgggaaca agaaaaatat atactgcaat
      121 agtgtatagt attcatttaa ataaaccaca gcactatcaa gtaaaatgta tagactctgt
      181 gctcgactct attcctattg ttactcctat tcaattacaa ttgtgggatt ggatagctaa
      241 atattatatg tgtactcgcg gcgaagtaat gaaagccgct ttgcctgcag gtttaaagtt
      301 agaaagcgaa gcattattgt attgtaatcc tatttttgat tctattgata cgttatcaaa
      361 taatgaactt atagtgtatt cagctgtaca aaacgctcaa ggtatttctt tagatgaatt
      421 aactaaaatt gtaaaaaaaa tacctgtaac aagaactgtg cacggtatgt tacaaaacaa
      481 agtattgctt acaggcgaaa aggttattca acaatataaa ccaaaaacag aaactcgaat
      541 tcagttggcc ggcagtattt ctgatgaaac atttgtaaac acatcctttg aaatggttgc
      601 taaagctccc aagcaattag aactatttac aaagtttctt gatttacata ttaagagtaa
      661 aaatcccgat ttttctattt taaaaaaaga attacttctt gttacaggtt gttcagcttc
      721 tattgtagca gaattaacaa agaagaatat actcgtagaa tgtaatacga acgtctctcg
      781 tttgcacaat acacatgttg aacttcaaga gtttaaaata ttaagtgaat cacagcaaaa
      841 ggctttacag caaataaata ctgcttttaa aacgcatgac acttgtttac tgcatggtgt
      901 tacggcaagc ggaaaaacag aagtatatat tgaacttata aaacaaacaa ttgctcaaaa
      961 taaacaagtc ttgtatttat tgcctgaaat tgctcttact tcgcaaataa tagatcgatt
     1021 gcaaaaagtg tttggaaata tagtcggagt atatcattct cgcttttcag acaatgaacg
     1081 tattgaggtt tggaacaatg tattatcatt atcaacctat aaaattatat taggagttcg
     1141 ttcttctata tttttgccct attcaaattt ggggcttatt atagtagatg aagaacatga
     1201 aacatctctt aaacagtttg accccgcacc tcgttataat gctcgcgata cagcaattat
     1261 actagctcaa ttgcataaag caaaaacgtt attgggaaca gctacaccaa gtttagaaac
     1321 atattataat gtattgcaaa aaaaatatgc attagttgaa ttaaatcaac gattttctca
     1381 ggtgcaattg cctcaaatac atatcataga tatggcaaaa gcacgtaaac aaaaacgaat
     1441 gtacaatgct atttttagta aagatttatt gaatcaaata gaactggcat tacaggaaaa
     1501 acaacaagtt attttatttc aaaacagaag ggggtttact cctttgatac agtgtacaga
     1561 ctgtggtaat ataccacatt gtaaacattg tgatgtaagt ttaacatatc ataaatactc
     1621 aaatacatta cgttgtcatt attgtggcta tgctatacaa tattcaccta attgtattgc
     1681 ttgtggaagt aatagacaag aatatcttgg ttttggtacc gaacaaattc aagatacatt
     1741 gcaagaaata tttcctcaca aacatatagc gcgtatggat ttagatacaa cacgaggtaa
     1801 atatgcatat catgaattaa ttgaacaatt tgaacataga gaaattgata ttttaatagg
     1861 tactcaaatg ataaccaaag ggttagattt tgaaaatgta ggtttggt
//
LOCUS       DAOTOW010000468         1900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47171_length_1900_cov_3.340379, whole genome shotgun sequence.
ACCESSION   DAOTOW010000468 DAOTOW010000000
VERSION     DAOTOW010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47171_length_1900_cov_3.340379"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="P5243_08230"
     CDS             <1..262
                     /locus_tag="P5243_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRS19477.1"
                     /translation="SEGFVNYPLAIKDVVFCAMFTEYENNTTKVSFRSKRQFPANEFS
                     AKYFNGGGHLNAAGGRRACTLQEAIEVFVSGLASFKEQLLKV"
     gene            complement(251..598)
                     /locus_tag="P5243_08235"
     CDS             complement(251..598)
                     /locus_tag="P5243_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRS19478.1"
                     /translation="MNIEEIREFCLQLPKTQECLPFNEYTLVFKVYNKMFALLNLDTG
                     NTINLKCNPEYALELREHYTAIIPGYHMNKKHWNTIYFEHIDSQLLKQLMYDSYNLVI
                     AQLPKKQQINLDF"
     gene            complement(595..>1900)
                     /locus_tag="P5243_08240"
     CDS             complement(595..>1900)
                     /locus_tag="P5243_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19479.1"
                     /translation="GSNQKGQLGTNDLFDRYYATRIDESLCKPYCAQTSIGNNTSYCT
                     PFSETIRTHLSPAQYSFTWYKNNNILPNTSDSLPISSSGIYKVIVQYISNECPSYTSE
                     IEITEKEKDYIPIIASYCDNNLHFKVVGNNSFTWYSSKFGYQLGTGNTLNVSRFFTEE
                     IIPDSIYQVWVQSENYCQRMPIQTIKNCECATFPPFAVDTSSCYNRTYFVHAIGDSVI
                     WHTDNNLQFPIAMQNTLYLENLGIGTHTLYATQVTNRCESAAIPVTLQLYYCDPWYTI
                     SGTVLANNERVSNSKVLLFESTSNTAIDSCNTNAMGEFTVYSHRNPAKIKAYSPNQYF
                     AHTWVGNKLEQEQAYEFIVDANIKGVKINLIPINSYIEKIEPETIDWTSISYIEIYNL
                     NGQKIGYTQSIQDIDSLTKKKTQYILRVYNQSHKYTTILWNK"
BASE COUNT          585 a          258 c          349 g          708 t
ORIGIN      
        1 ttctgaagga tttgtgaatt atccgcttgc aataaaagat gtggtgtttt gtgctatgtt
       61 taccgaatat gaaaataata caactaaggt ttcttttcgg tcaaaacgac aattccctgc
      121 aaatgagttt tcggcaaaat attttaatgg cggcggacat ttgaatgcag cagggggtag
      181 gcgagcttgt acgttacaag aagcaattga agtttttgtt tcaggacttg cttcgtttaa
      241 agaacaatta ttaaaagtct aaattaattt gttgtttttt tggtaattga gctataacta
      301 agttatacga atcatacatt agttgcttta gtaattgtga atcaatgtgt tcaaaatata
      361 tagtattcca atgttttttg ttcatatgat atccgggaat tatagcagta tagtgttctc
      421 gtaattctaa ggcatattca ggattacact tcagattgat tgtgtttccg gtatctaaat
      481 tgagcaaagc aaacattttg ttgtatactt taaaaacaag tgtatattca ttaaaaggta
      541 agcattcttg tgttttcggt aattgtaaac aaaattctcg tatttcttct atgttcattt
      601 gttccacaaa attgtagtat acttatgtga ttgattatat actcgtaaaa tatattgggt
      661 tttctttttt gtcagagaat caatatcttg aatcgattgt gtgtatccaa ttttttgacc
      721 attgagattg taaatttcta tatacgaaat agaagtccaa tctatagttt caggttctat
      781 tttttctatg tatgaattta tgggaattaa atttattttt actcctttaa tgttagcatc
      841 tactataaat tcataagctt gttcttgttc tagtttatta ccaacccatg tatgagcaaa
      901 gtattgatta ggagaatatg ctttgatttt tgccggatta cgatgagaat ataccgtaaa
      961 ttctcccata gcatttgtat tgcatgaatc tatagcagta ttagaggttg attcaaatag
     1021 tagtactttg gaattgctta ctcgttcatt gtttgctaat actgtacctg aaattgtgta
     1081 ccaagggtca caataatata attgcagtgt tacaggtata gccgcacttt cgcatctgtt
     1141 tgtaacttgt gtggcatata gagtatgagt tcctatacct aaattttcga gatatagggt
     1201 attttgcata gcaataggaa attgtaagtt attgtcggta tgccaaatta cactgtctcc
     1261 aatagcatgt acaaaatatg ttctattgta acatgaagaa gtatctactg caaatggtgg
     1321 aaaagtagca cattcacaat ttttaatagt ttgaataggc atacgttgac agtaattttc
     1381 tgactgcacc cacacttgat atatactatc gggaattatt tcttcggtaa aaaaacgtga
     1441 tacattcaac gtatttccgg tacctaactg gtatccgaat ttactgctat accatgtgaa
     1501 ggagttgttg cctacaactt tgaaatgtaa gttattgtca caataagagg caattatagg
     1561 aatataatct ttttcttttt cggtaatttc aatttctgag gtatatgacg gacattcatt
     1621 tgaaatatat tgtacgatta ctttataaat tccactactt gaaataggta gagagtcaga
     1681 agtgtttggt agtatattgt tgtttttgta ccacgtaaat gaatattgtg cgggagataa
     1741 atgagtgcga atagtttcag aaaatggagt gcaataagag gtattatttc caatagatgt
     1801 ttgagcacaa tagggtttac aaagtgattc atcgatacgt gttgcgtagt atctgtcaaa
     1861 taaatcgttt gtacctaatt gtcctttttg attagaacca
//
LOCUS       DAOTOW010000469         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47232_length_1898_cov_3.761259, whole genome shotgun sequence.
ACCESSION   DAOTOW010000469 DAOTOW010000000
VERSION     DAOTOW010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47232_length_1898_cov_3.761259"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="P5243_08245"
     CDS             complement(<1..505)
                     /locus_tag="P5243_08245"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19480.1"
                     /translation="MKKVVLLLVSVFMGMSMFAQSEYPDSPYDKPHIADKKPIILQTI
                     RYADVMWSKKIERTLPLREKLNHPLYFPNNESGKIGSRYSLTALTINGIKLGYFQAYD
                     PMSDGAVPISEKDLDSKLGAMIDTISIENEDGTVSYKPVEKQVDPSSIKYLILREEWI
                     FDKQRSVM"
     gene            complement(532..>1898)
                     /locus_tag="P5243_08250"
     CDS             complement(532..>1898)
                     /locus_tag="P5243_08250"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRS19481.1"
                     /translation="TIIAYADAIPKEKLFKLPNDLRYYIVDEDTKDTLMLEQKVNNKD
                     NTDVPAQIMVGPDPSGDKGHAKFLKADVDNYKKWALQVISEFGADSLSPLAKGIKTTF
                     NTDPVKGSGHESGMLSWANRNFEHLPLMAVVTILTQMQAAVRNVEGDILNLMYGQLDA
                     TSFKFNKLVPVVLPNSTYVMQGNEYNAQIFLAAFDTTAPPTVLVNGRQIELDPKTNLP
                     TYKTTGGGVGPQKYKAEIKIKQPNSDNYNSYFVESEYQVAKPSLVVSPDKMNVFYIGP
                     DNPVSISVPGVPSDKITAVLTPAGFGTITKNPQGGYIVRVTRPGKCQIAVTADFDGKK
                     QNMGAVEFRIKSVPDPVAQVLGMEGGDVGKERLQAAATVDAKMKDFDFDLKFEVESFR
                     VSAKIGAYFVEEASQNNRITQNMKQNIFTKVTKGNKVYFEDIKARGPDNKPRSLGVLL
                     FKIN"
BASE COUNT          594 a          343 c          259 g          702 t
ORIGIN      
        1 ccattacaga tctttgttta tcaaaaatcc attcttcacg taaaattaga tattttatag
       61 aactagggtc tacttgcttt tcaacaggtt tatatgatac tgttccatct tcattttcaa
      121 tagatatagt atcaatcata gctcctaatt tagaatctaa atctttttcg gaaattggta
      181 cagcaccatc actcatagga tcatatgctt gaaaatatcc taatttaata ccatttattg
      241 ttaatgctgt caaactatat cttgaaccaa tttttcctga ttcattatta ggaaaatata
      301 aaggatggtt taatttttca cgcaaaggta atgtacgttc aatcttttta gaccacataa
      361 catcagcgta tctgattgtt tgcagaataa ttggtttttt atctgcaata tgtggtttat
      421 catacggact gtcaggatat tcactttgag cgaacattga cattcccatg aaaacactta
      481 cgagtaataa aacaactttt ttcatagtat gtgtgtttta agttaaatta cttaattaat
      541 tttgaataac aataccccta agctacgtgg tttattatct ggtcctcgtg ctttaatatc
      601 ttcaaaatat accttattac ctttagttac tttagtaaat atattttgtt tcatattttg
      661 agtaattcta ttgttctgag atgcttcttc aacgaaataa gctccaatct tagcacttac
      721 tctaaatgat tcaacttcaa attttaaatc aaaatcgaaa tctttcattt tagcatctac
      781 agttgccgca gcttgtaatc tttctttacc aacatctcca ccttccattc caagaacttg
      841 agcaacagga tccggaactg atttaatacg aaattcaacg gcacccatat tttgtttttt
      901 tccgtcaaaa tcagcagtta cagcaatttg acattttcct ggtcttgtta cacgaacaat
      961 ataaccacct tgtggatttt ttgtaattgt tccaaaacct gctggggtta atactgccgt
     1021 aattttatct gaaggtactc caggtactga aatagaaacc ggattatcag gacctatata
     1081 aaatacattc attttatcag gtgaaacaac caaacttggt tttgcaactt gatattcact
     1141 ctcaacaaaa taagaattat aattatcgga atttggttgc ttgattttaa tttctgcttt
     1201 atatttttga ggacctactc caccgccagt tgttttgtac gttggtaaat ttgttttagg
     1261 atctaattct atttgtcttc cgtttactaa tactgtagga ggtgctgttg tatcaaatgc
     1321 agctaagaaa atctgagcat tatattcatt tccttgcatt acataagtac tattaggcaa
     1381 tacaacaggc actaatttat taaatttaaa tgaagttgca tctaattgac catacattaa
     1441 atttaaaata tcaccttcaa cattacgaac agccgcttgc atttgtgtta aaattgtaac
     1501 aacagccatc aagggtaaat gttcaaaatt tctgttagcc catgataaca ttcctgattc
     1561 gtgaccacta ccttttaccg gatcagtatt aaaagtagtt ttaatacctt tagcaagagg
     1621 tgataacgaa tctgctccaa attcagaaat tacttgcaat gcccatttct tataattatc
     1681 aacatcagct tttaagaatt tcgcatgacc tttatctccg cttggatcag gcccaaccat
     1741 aatctgagca ggtacgtcag tattatcctt attatttact ttttgttcaa gcattaaagt
     1801 atctttagta tcttcatcaa ctatataata ccttaaatca tttggtaatt tgaataattt
     1861 ttctttaggt atagcatctg catatgcaat aattgtat
//
LOCUS       DAOTOW010000470         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47246_length_1898_cov_2.836679, whole genome shotgun sequence.
ACCESSION   DAOTOW010000470 DAOTOW010000000
VERSION     DAOTOW010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47246_length_1898_cov_2.836679"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5243_08255"
     CDS             <1..482
                     /locus_tag="P5243_08255"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS19482.1"
                     /translation="TKNEMEDDTVKDGMDMSICVFHKTKGILEYAGAFNPLYIIRDGK
                     IMTIKGDRFSVGLGNEHEDVPFKNHIIKVQKGDTFYLFTDGYADQFGWQTGKKMKFRR
                     FRHIILSVYKLPFTKQVKALRDALQDWKGDLEQIDDILIVGFKLDNYLDAMYSKPTE"
     gene            489..1256
                     /locus_tag="P5243_08260"
     CDS             489..1256
                     /locus_tag="P5243_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRS19483.1"
                     /translation="MTQIISYNVNGIRSAIDKGFIEWLHIQNPDILHIQEVKALKEQI
                     NTEIFKTLGYTLYWFAAQKKGYSGVATLTKNTPTHIEYGIGNNFFDNEGRFIRLDYPN
                     YTLINSYFPSGSSGDLRQQKKEEYLELIFNYCKELIQKQPNIIISGDFNICHTPIDIN
                     HPERHTGYSGFLPQEREWMDKLMHLGFVDSFRIFNTQPHNYSWWSYRAGARSKNLGWR
                     IDYHLVSTSLRNAITHASILPHIIHSDHCPVVIEIQL"
     gene            1432..>1898
                     /locus_tag="P5243_08265"
     CDS             1432..>1898
                     /locus_tag="P5243_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HRS19484.1"
                     /translation="MNKNLKILLSLPLIFISLGAFAQGTDTDHGITSWMLTSTALVLL
                     MVPGLAMFYGGLVRTKNVLGTMMHSFTAMGVMTILWAAVGYSMSFGGNILGGWFGWSP
                     DYFFLQGIDNSIIKDDAGMGIIPEYIFTMFQGKFAIITPAILAGAFAERVTFKG"
BASE COUNT          695 a          278 c          319 g          606 t
ORIGIN      
        1 taaccaaaaa cgaaatggaa gacgataccg taaaagacgg tatggacatg tcaatatgtg
       61 tatttcataa aaccaaaggt attttagaat atgcaggagc atttaatcct ctgtatataa
      121 tacgcgacgg aaaaataatg acaatcaaag gagacagatt ttctgtagga ttaggaaatg
      181 aacatgaaga tgttcctttc aaaaatcata ttataaaagt tcaaaagggc gatacttttt
      241 atttatttac cgatggatat gcagatcaat ttggatggca aaccggcaaa aaaatgaaat
      301 tccgcagatt tagacacatt attttatctg tttacaaact tccttttact aagcaagtaa
      361 aagccttacg agatgcgtta caagattgga aaggtgattt agaacaaata gacgatatac
      421 taattgtagg atttaaactt gataattatt tagatgctat gtattcaaag ccaactgaat
      481 aatgtactat gacacaaata atttcataca atgtaaatgg tattcgttct gcaatagaca
      541 agggatttat agaatggcta cacattcaaa atcctgatat attacatata caagaagtaa
      601 aagctcttaa agaacaaata aataccgaga tttttaaaac cttaggctat acgctatatt
      661 ggtttgctgc acaaaaaaaa ggatatagcg gtgtagctac attaacaaaa aatacaccta
      721 cacatataga atatggtatt ggcaataatt ttttcgataa cgaaggcaga tttattcgat
      781 tagactaccc taattataca cttattaatt catattttcc ttcggggtca tcaggcgact
      841 taaggcaaca aaaaaaagaa gaatatttag aacttatttt taattattgc aaagaattaa
      901 ttcaaaaaca accaaatatc attatatcag gtgattttaa catatgccat acccctattg
      961 atattaatca tccggaacgt catacaggtt attccggttt tttaccacaa gaaagagaat
     1021 ggatggataa attaatgcat ttaggttttg ttgatagttt tagaatattt aatactcaac
     1081 ctcacaatta ttcttggtgg agttacagag caggagcaag aagcaaaaat ttaggctgga
     1141 gaattgacta ccatttggta agcacatcat tacgcaacgc tataacacat gcaagtattt
     1201 tacctcacat aatacattcc gatcattgtc cggtagtgat tgaaatacaa ttataatcaa
     1261 cgtaactcta atttatttct aagtaaaaca cgcaaataaa atattaaaaa ttaagtaatt
     1321 ttaccatttt aattacatat aataattttt taagcaatat aaattgacat ttataaccat
     1381 ttttattatt tttgctttcg gattataatt actaactaaa ttttatttac aatgaacaaa
     1441 aatctcaaga ttttattaag tcttccattg atttttatat cgctgggggc ttttgcacaa
     1501 ggaacagata ccgatcatgg tattacatcg tggatgttaa catcaacagc attggtatta
     1561 ttaatggtac ctggattagc aatgttttat ggcggtttgg tacgaaccaa aaacgtattg
     1621 ggaacaatga tgcatagctt tactgcaatg ggggttatga ctatattgtg ggcagcagta
     1681 ggatactcta tgagttttgg tggtaatatt ttaggtggtt ggtttggatg gagtcccgac
     1741 tatttctttt tacaaggaat agacaattct attataaaag acgatgccgg catgggaata
     1801 atacccgaat acatttttac catgtttcaa ggtaaatttg caattataac accggctata
     1861 ttagcaggag catttgcaga acgcgtaaca tttaaagg
//
LOCUS       DAOTOW010000471         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47347_length_1895_cov_3.450543, whole genome shotgun sequence.
ACCESSION   DAOTOW010000471 DAOTOW010000000
VERSION     DAOTOW010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47347_length_1895_cov_3.450543"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="P5243_08270"
     CDS             complement(<1..548)
                     /locus_tag="P5243_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19485.1"
                     /translation="MNTSFKILIAEDSEVVQMSIHDFLIKDLQHTYSVLKAYNGREAC
                     NMAYKHKPDIILIDIEMPVMDGIEAISIIKKNKEIAHIPIIVMSGTRNFKKAMEAGAN
                     DFLLKPINQYELLLRITTNIISSRNDIELKKQQEILANQQFEVIAQRDMILMQQNEMY
                     EDIRYARAIQNAILSDPEILNK"
     gene            complement(568..975)
                     /locus_tag="P5243_08275"
     CDS             complement(568..975)
                     /locus_tag="P5243_08275"
                     /inference="COORDINATES: protein motif:HMM:NF002007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRS19486.1"
                     /translation="MSLLTWNSTYSVNIVEIDNQHKKLIDLINQLYDAKQAGAADTII
                     QKVFNELVSYTFTHFNYEESLLRKANYSDLAEHCDIHKSIVERVRNLQKQYNDGSATA
                     INDTLEFMKNWLMKHIAGTDKKYSQELNAAGIR"
     gene            complement(994..1401)
                     /locus_tag="P5243_08280"
     CDS             complement(994..1401)
                     /locus_tag="P5243_08280"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRS19487.1"
                     /translation="MALIIWNNMYSVNVESLDTQHKKLVELVNQLHDSIKSGQKDKVL
                     TGIYNELIGYTITHFRDEEALMRKAGYADLEDHIDIHRSLVERVKKLQKDYFDGNPNA
                     WNETLEFLRNWLLKHIAGTDKKYSQALNQAGIV"
     gene            complement(1419..>1895)
                     /locus_tag="P5243_08285"
     CDS             complement(1419..>1895)
                     /locus_tag="P5243_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19488.1"
                     /translation="AIVFSKITTEPITLKLHVRYGENIPDITAPGGFAVKEVIDTITG
                     ECAYTPLKSMSYWGEIHTNGKNWEFGVFGGMYTNTGTKESMSSVTNSVHGLATNIAAM
                     YRISPRVIYKINKFSVGAEIEYTAALYGKNYDANYIPATTSEISNTRILLSTIYAF"
BASE COUNT          562 a          306 c          300 g          727 t
ORIGIN      
        1 aatttattaa gaatttcggg gtcagagaga attgcatttt gaatagcacg ggcatagcga
       61 atgtcttcat acatttcatt ttgttgcatt aaaatcatat cgcgctgagc tataacttca
      121 aattgctggt ttgcaagaat ttcttgttgt ttttttagtt caatatcatt tctagatgaa
      181 ataatattag ttgtaattct caaaagtagt tcgtattggt ttataggttt tagtaaaaaa
      241 tcattggctc cggcttccat agccttttta aagtttcgtg tccccgacat tactataatt
      301 ggtatatgag ctatttcttt atttttttta ataatagaaa tagcttcaat accatccatt
      361 accggcattt ctatgtctat taatataata tcgggtttgt gtttgtaagc catattacat
      421 gcttcacggc cattgtatgc ctttaacaca gaatatgtat gttgtaaatc ttttattaaa
      481 aaatcatgaa tactcatctg aacaacttca gaatcttcgg ctataagtat tttaaatgaa
      541 gtattcataa ctttttattt aggaatatta gcggattcct gcagcattca attcttgtga
      601 atattttttg tcggtgcctg caatatgttt cataagccag tttttcataa attctagcgt
      661 atcattaatt gcggtagcac tgccatcgtt gtattgtttt tgaagattac gtacccgttc
      721 tacaatactt ttatgaatat cgcagtgttc agccaaatca gaatagttag cttttcgtaa
      781 caaactttct tcataattaa agtgtgtaaa tgtatacgaa actaattcat taaagacttt
      841 ttgaataata gtatcagcag ctcccgcttg ttttgcatca tacaattgat ttattaaatc
      901 aataagtttt ttgtgttgat tatctatttc aacaatattt accgaatatg ttgaattcca
      961 tgttaataat gacatagtat cataaattta aaattaaaca ataccagctt gattaagagc
     1021 ttggctatat tttttgtcag ttcctgctat atgttttagg agccagttgc gaagaaattc
     1081 taatgtttcg ttccacgcat taggattgcc gtcgaaatag tctttttgta atttctttac
     1141 acgttctact aaactacggt gtatatctat gtgatcttct aaatcggcat atcctgcttt
     1201 acgcataaga gcttcttcgt ctctaaagtg tgtaatggta tagccaatga gttcgttata
     1261 aataccggtt agtactttgt ctttttgacc ggatttaata gagtcgtgta attggttcac
     1321 taattcaacc aattttttgt gttgagtatc taaagactca acattcacag aatacatgtt
     1381 gttccaaatt attagtgcca tatgaattta gttttaagtt agaatgcata aatagtagat
     1441 agtaatatac gagtattact tatttcactt gtagttgccg gtatgtaatt agcatcgtaa
     1501 ttttttccat acaatgcagc tgtatactct atttcagcac ctacactaaa tttatttatt
     1561 ttgtatatta ctcttggcga aattctatac atagcggcaa tgttggtagc taaaccatga
     1621 acagaatttg ttacgctact catagattct ttagtgcctg tatttgtata cattcctcca
     1681 aatactccaa attcccaatt tttaccattg gtatgtattt cgccccaata agacatgctt
     1741 ttgagtggag tataagcaca ttctccggtt atagtatcaa ttacttcttt tacggcaaaa
     1801 ccaccgggag cagtaatatc gggaatgttt tctccatatc gtacatgaag tttcaatgta
     1861 ataggttctg tagtaatttt tgaaaataca atggc
//
LOCUS       DAOTOW010000472         1891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47473_length_1891_cov_3.616558, whole genome shotgun sequence.
ACCESSION   DAOTOW010000472 DAOTOW010000000
VERSION     DAOTOW010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47473_length_1891_cov_3.616558"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="P5243_08290"
     CDS             <1..1758
                     /locus_tag="P5243_08290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRS19489.1"
                     /translation="YAIDEAHCISEWGHDFRPEYRRIKPIVESIGRAPIIALTATATP
                     KVQHDIQKNLGMLDATVFKSSFRRENLYYEVRPKVSASKQIIRFIKDNPGKSGIIYCL
                     SRKKVEELSETLVVNGIKALPYHAGLDAHTRSRNQDMFLMEDVDVIVATIAFGMGIDK
                     PDVRFVIHYNMPKSLEGYYQETGRAGRDGGEGKCISFYSYKDITKLEKFMQGKPVAEQ
                     EIGKQLLLETSSYAESSVCRVRQLLNYFGEVMTEDCGNCDNCLNPKTQFEGKEYVKLA
                     LETILAVKERFKAGHVAEILAGKVSSEIKTYHHDKLEIFGAGNEKDVKFWNAVLRQLL
                     IARLIDKEIENYGLLKITAKGKEFLQRPYSILITEDHDYEEADGDLNLGQGTGSLDPV
                     LISMLIELRKKIAKKHNVPPFVVFQETSIEDMAVQYPITIEELTNIIGVGQGKAQKYG
                     KEFVELIAQYVADNNIERPQDMVVKSVVNKSGNKVQIIQNIDRKVSLDTLAESKGLTM
                     VELLDEIERIVNSGTKINIDYYIKEVMEEDVVEEIYDYFRTSESESVQDAIDELTQDD
                     YSEEEIRLVRIKFLSELGH"
BASE COUNT          673 a          257 c          377 g          584 t
ORIGIN      
        1 tatgcaatag atgaagctca ttgtatttct gaatggggac atgattttag accggaatat
       61 cgtcgaataa aacctattgt agaatcaata ggaagagccc ctattattgc tcttactgca
      121 actgctactc ccaaagtgca gcatgatatt cagaaaaact tgggaatgtt agatgctacc
      181 gtttttaaat catcgtttcg tagagaaaat ttatattacg aagtgcgtcc caaagtaagt
      241 gcttctaaac aaataatacg ttttatcaaa gataatccag gtaaatcggg tattatttat
      301 tgtttgagcc gaaaaaaggt agaagaatta tcagaaacat tggttgtgaa tggaattaaa
      361 gcattaccct atcatgcagg gcttgatgct catacacgtt cgcgaaatca ggatatgttt
      421 cttatggaag atgttgatgt aattgtagct actattgctt ttggaatggg tattgacaaa
      481 cccgatgtgc gatttgtaat tcattataat atgcccaaaa gcttagaggg gtattatcaa
      541 gaaacgggaa gagccggtcg tgatggtggt gaaggtaaat gtatttcatt ctatagctat
      601 aaagatatta caaaacttga aaaatttatg caaggcaaac ctgttgccga acaagaaata
      661 ggtaaacagt tgttgctcga aacttcttca tatgccgaat caagtgtatg tagagttcgt
      721 caattgctaa attattttgg tgaagtaatg acagaggatt gtggtaattg tgataattgt
      781 ttgaatccaa aaacacaatt tgaaggtaaa gaatatgtaa aacttgcttt agaaactatt
      841 cttgctgtaa aagaaagatt taaagccggt catgtcgccg aaattcttgc aggaaaagta
      901 agttctgaaa ttaaaacata tcatcatgat aaactcgaaa tttttggagc aggaaacgaa
      961 aaagatgtaa agttttggaa tgctgttttg cgtcaacttc ttatagcacg attgatagat
     1021 aaagagattg aaaattatgg attgttaaaa ataacagcaa aaggtaaaga atttcttcaa
     1081 cgtccatatt caattttgat aacagaagac catgattatg aagaagctga tggcgatttg
     1141 aacttaggac agggtaccgg ttcgcttgat cctgtgctta tttctatgct tattgaatta
     1201 cgcaagaaaa tagcaaaaaa acataatgta cctccttttg ttgtgtttca agaaacatct
     1261 attgaagata tggcagtgca atatcctatt actattgaag aacttactaa tataataggg
     1321 gtaggacaag gtaaagccca aaagtatgga aaagagtttg ttgaacttat tgctcaatat
     1381 gttgctgaca ataacataga acgtccgcaa gatatggtgg taaaatcggt tgtaaataaa
     1441 tcaggtaata aagttcaaat aattcaaaat attgaccgca aagtgtcact tgatactctt
     1501 gctgaatcca aagggttaac tatggtagag ttacttgatg aaattgaacg tattgtaaat
     1561 tctggtacta aaataaacat agattactat ataaaagaag taatggaaga agatgtggtt
     1621 gaagaaattt atgattattt tcgtacttcc gaatcagaat cggttcaaga tgccatagat
     1681 gaacttaccc aagacgatta ttctgaagaa gaaattagat tagttcgtat taaattttta
     1741 tcagaattag gtcattaatt atttttttat taccaatgta tgagaatagc tgtagatttt
     1801 gacggtacta tagtagagca taaatatccc gaaattggag aaattaaatt gtttgccttt
     1861 gaaaccctgc gtgaacttca aaaacaaggg c
//
LOCUS       DAOTOW010000473         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47509_length_1890_cov_3.942234, whole genome shotgun sequence.
ACCESSION   DAOTOW010000473 DAOTOW010000000
VERSION     DAOTOW010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47509_length_1890_cov_3.942234"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="P5243_08295"
     CDS             complement(<1..783)
                     /locus_tag="P5243_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS19490.1"
                     /translation="MQTLSHKTIALLVAGIASAISIVAVLVSGFVIAQNNTIPYAIFI
                     ALTMFAVIYIITFYITKEYIIQKVKPIYKTIHSAQTSTLKKITENEHEESNIIATTEK
                     EVAEWAKRKIIEIEELKRLEKYRKEYIGNVSHELKTPIFSIQGYISTLLDGGLYDETI
                     NKAYLEKADKAIQRMTSIVRDLDSITRLEAGELKLEYSQFNIILLVKEVFEAQELLAK
                     ERNVHMYIDMGVEKPIMVNADKKRIQMALNNLVINAIKYNNNN"
     gene            977..1477
                     /locus_tag="P5243_08300"
     CDS             977..1477
                     /locus_tag="P5243_08300"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRS19491.1"
                     /translation="MNISIIAAIDENYGIGKNNALLWHLPADLKFFKAKTTGHSIIMG
                     RNTFESIGGGRPLPNRTTIIITRNREYSAPQGCIIVHSLEQALTFCSNEDEVFVCGGA
                     QIYELALPIAHTMYITHVHTQCNADTFFPKWNTHDWVTISKNKCTADEKHTFDFTFAE
                     YRRKNL"
     gene            1484..>1890
                     /locus_tag="P5243_08305"
     CDS             1484..>1890
                     /locus_tag="P5243_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19492.1"
                     /translation="MEHNKTYICFFNSHKPWGGGEKWHSEIASYLFSKNHNVIVCGNP
                     HGALLQSIDSKIPQYTITLRNLSFLNPFKYVACYRMFKKYRVKTVILCLPIDVKVAGI
                     AAKLAGVQNIVYRRGCAIPIKNSFTNRFLFSHI"
BASE COUNT          656 a          286 c          269 g          679 t
ORIGIN      
        1 attattatta ttatatttta ttgcattaat tactaaattg ttcaatgcca tttgtatcct
       61 ttttttatcg gcatttacca taataggttt ttcaaccccc atatctatat acatatgaac
      121 atttcgttct tttgcaagca attcctgagc ttcaaaaact tcttttacca aaagtattat
      181 attaaactgg ctgtattcta attttaactc acctgcttcg agtctggtaa tagaatctaa
      241 atcacgtaca attgaggtca tacgttggat ggctttatca gccttttcca aataagcttt
      301 atttattgtt tcatcataca aaccaccatc taataatgtt gatatatatc cttgtatact
      361 aaaaattggc gtttttaatt cgtgtgaaac atttccaata tactctttac gatatttttc
      421 caatcgtttt aattcttcta tttctataat ttttcgtttt gcccattctg caacttcttt
      481 ttctgttgtt gcaattatat tagattcttc atgttcattt tctgtaattt tttttagtgt
      541 tgacgtttga gccgaatgta tggttttgta tataggcttt actttttgaa taatatattc
      601 ttttgtaata taaaatgtta taatgtagat aacggcaaac atagtaagag ctataaaaat
      661 agcatacgga atagtattat tttgtgctat tacgaaaccc gaaaccaata cggctactat
      721 tgatatagct gaagctattc cggcaactaa aagggcgata gttttgtgtg aaagagtttg
      781 catatactaa atacattttg atataagtaa aagtagtatt tttttgcaaa ttgaatacgt
      841 atacgtgtta aatttatgtt aactcaatca tgatacccaa atatataaga aacctatatt
      901 ttatttcaaa ataaaagtaa aaacaagaaa tttcattact tttgattctc attcttagaa
      961 aatgaaaaca aacaaaatga atatttcaat tattgctgca attgacgaaa attatggaat
     1021 tggtaaaaac aatgcgttat tatggcattt acctgccgat ctcaaatttt ttaaagcaaa
     1081 aactacaggt cattctataa ttatgggtcg taatacattt gaatcaatag gtggtggacg
     1141 acctttaccc aacagaacta caataattat taccagaaat agagaatatt cagctccaca
     1201 ggggtgcata attgttcatt cacttgaaca agctcttact ttttgcagta acgaagatga
     1261 agtatttgta tgtggaggag ctcaaatata tgaacttgca ctacctatag cacatactat
     1321 gtatattaca catgttcata cccaatgcaa tgccgataca ttttttccaa aatggaatac
     1381 tcatgattgg gttacaattt caaaaaataa atgtactgcc gatgaaaaac acacatttga
     1441 ttttacattt gcggaatata gaagaaaaaa tctataacat agtatggaac ataacaaaac
     1501 atatatatgt ttttttaata gtcacaaacc atggggaggg ggcgaaaaat ggcatagtga
     1561 aatagcatca tatttatttt caaaaaatca taatgttata gtatgcggca accctcatgg
     1621 tgcattatta caatcaatag attctaaaat tcctcaatat acaataacat tacgcaatct
     1681 tagtttttta aatccattta aatatgtagc atgttacaga atgtttaaaa aatatagagt
     1741 taaaaccgta atattgtgtt taccaattga tgtaaaagta gcagggatag cagctaaatt
     1801 agccggagta caaaacattg tttacagacg agggtgtgcc attccaatta aaaattcatt
     1861 cacaaatagg tttttgtttt cgcatattat
//
LOCUS       DAOTOW010000474         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47516_length_1890_cov_2.491008, whole genome shotgun sequence.
ACCESSION   DAOTOW010000474 DAOTOW010000000
VERSION     DAOTOW010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47516_length_1890_cov_2.491008"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1890
                     /locus_tag="P5243_08310"
     CDS             <1..>1890
                     /locus_tag="P5243_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRS19493.1"
                     /translation="ELEKFNKFLNICKDVDSLPGVDGLISLGQAVKIEGSSVVRYFDT
                     LPQTQQQLDSISRELIQQKLYQGFLFNDTSHVYLIALTINKKVLDSPARESLIQNIED
                     TFNSFAHQNYDKVFYTGLPYIRTKISLKLRSELTMFILFAALVCALILFVFFRSFKIV
                     AFSMLVVGVGVIWVMGWMGIFGYDITILNALLPPLIIVIAVPNCVFFLNKYHQEYAIH
                     GNKIKALQRVVRKIGNAIFLSNLTTAAGFATFVITDSELLQQFGIIAFIGIICVFIFS
                     LTLIPIIFSFVSPPNQRNLKHLENKFLQKVIASISHLVAHKRNYIYGGAITIFIVAII
                     GISQIISTGYMVDDLPHDDPILTDLQFVEKHFNGVLPIEIVIESNEKINLIRDKGFLY
                     KMEALHDTLLNYTEVSKPLSIVEFIKFAWQAHNGGNPEYYTLHNSGDIFFQNKMKRIV
                     RGQNSAHLQYPLIDSTGKKIRLKCNVKDIGTKKMQELETNLKADLDSIFPKSEYKTQI
                     TGSSIVFFKGTKYLVSSLFSSVALAIVLITFFMAWLFRSKRMVMISLLPNILPQLFTA
                     ALMGYLGIPIKPSTILVFSIAFGISVDDTIHFLAKYRQELTHTNWNIGKSVMIALRET
                     GSSMI"
BASE COUNT          616 a          271 c          316 g          687 t
ORIGIN      
        1 ttgaattaga aaaattcaat aaatttttga atatttgtaa agacgttgat tcattaccgg
       61 gagttgatgg acttatttca ttggggcaag ctgtaaaaat tgaaggttct tctgttgtgc
      121 ggtattttga cacattgccc caaacacagc aacaacttga ttctatttcg cgtgaactta
      181 tacaacaaaa attatatcaa ggtttcttat ttaatgacac atctcatgta tatttaatag
      241 ccttaacaat aaataaaaaa gttcttgaca gtcctgcacg agaatcttta attcaaaata
      301 ttgaagatac gtttaattct tttgctcatc agaattatga taaagtattt tataccggct
      361 tgccatatat tcgtaccaag atatcattaa agcttcgcag tgaacttacc atgtttattt
      421 tgtttgctgc actggtgtgt gcattaattt tgtttgtgtt tttccgttct ttcaaaattg
      481 tggcattctc aatgctggta gtaggtgtag gggtaatatg ggtaatggga tggatgggga
      541 tttttgggta tgatattaca atactcaatg ctttgttgcc acctcttatt atagttattg
      601 ctgttcctaa ttgtgtgttt ttcttaaata aatatcatca agaatatgcc attcatggaa
      661 ataaaattaa agcattacaa cgagttgtgc gaaaaatagg taatgcaata tttttatcaa
      721 accttacaac agctgccggt tttgctacat ttgtaattac cgatagtgaa ttgttgcagc
      781 agtttggaat aatagcattt attggaatta tttgtgtgtt tattttctca ttaacattaa
      841 tcccaattat atttagtttt gtttctcctc ctaatcagcg taatctaaaa catttagaaa
      901 ataaattttt acaaaaagtt attgcatcaa tttctcattt agttgctcat aaacgtaatt
      961 atatttatgg aggagcaatt actattttta ttgtggcaat tattggaatt tctcaaatta
     1021 tcagtaccgg atatatggta gatgatttgc cgcatgatga tcctatttta actgatttac
     1081 aatttgttga gaaacatttt aacggagtat tgccaataga aatagttata gaatctaatg
     1141 aaaagataaa tcttattcgt gataaaggct ttttgtataa aatggaagcg ttacacgata
     1201 cattacttaa ttatactgaa gtctcaaaac ctctttcaat agttgagttt attaagtttg
     1261 catggcaagc acacaatggc ggaaatccag aatattacac cttgcataat agtggcgaca
     1321 tattcttcca aaataaaatg aaacgaatag tgcgaggaca aaatagtgca catttgcaat
     1381 atccacttat tgattctaca ggtaaaaaaa ttcgattaaa gtgtaatgta aaagatattg
     1441 gaacaaaaaa aatgcaagaa ttagaaacaa atttgaaggc tgatttagat tcaatttttc
     1501 caaaatctga atacaaaact caaattaccg gttcaagtat tgtgtttttt aaaggtacaa
     1561 aatatttggt aagtagttta ttctcaagtg ttgctttggc tatagtactc atcacatttt
     1621 ttatggcgtg gttgttccgt tccaaacgta tggttatgat ttcattactg ccaaatattc
     1681 ttccgcaatt gtttactgcc gcacttatgg gatatttagg aattcctatt aaaccatcta
     1741 ctattcttgt gtttagtata gcatttggta tatcggtaga tgatactatt cattttcttg
     1801 caaaatatcg tcaagaatta actcatacca actggaatat tggaaaatct gttatgatag
     1861 ccttacgaga aacagggtca agtatgattt
//
LOCUS       DAOTOW010000475         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47617_length_1887_cov_2.891376, whole genome shotgun sequence.
ACCESSION   DAOTOW010000475 DAOTOW010000000
VERSION     DAOTOW010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47617_length_1887_cov_2.891376"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..508)
                     /locus_tag="P5243_08315"
     CDS             complement(20..508)
                     /locus_tag="P5243_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19494.1"
                     /translation="MSKKFIYIILIVIGLQPAFSQTPHLLHAQVAYDYGYYDSVFTYI
                     SPHNSQPEYAELCGKTYFATAMYTQALQCFSLAHTPMSYYYIASIYAIQQQTDSALIY
                     LRKHLLSTHKLQYSTVITNPAFNSISKTQEWREFWKTTYYTELDLLIENIKYSLQNNR
                     KS"
     gene            complement(501..>1887)
                     /locus_tag="P5243_08320"
     CDS             complement(501..>1887)
                     /locus_tag="P5243_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS19495.1"
                     /translation="QPSYSYIHTVPGSQHNLYDSNNGIGLSPGFTYCYRILSHYSDNF
                     PSYISPEICVELKPTIHVITNVSVQKTHKNQGEIFIAWSLPRDFDSITYPKPYQYKIY
                     RSFGYFGTNLQEIGTIQGLYDTTFIDTGINTTDSVFSYKIELYSLASGSPILIGNPPI
                     CSSPFVTLIPQNKKITLRVNAQVGWQNDSIYVYRKELNSDIFKYIGICNGQEFIDSGL
                     VNLQEYCYYVKTSGYFTNAQLPQRVYNFSQIACTYPTDTVPPKAPKFVINQNCNALSR
                     TISWTIDTTDEIETVNIYYKHCKTNSYTLLASLSKLQTQFIHTFTDTVRSMAGCYYMT
                     CIDSAGNESTIRNDTCLYNCPLYILPNIFTPNADGQNDVYHPTVNRFVEYVDMKIYDS
                     WGNLVFETKNPSILWKGFNGKTKKPLDDGVYYYVCDVYEYWADCNIHPRTLVGFIHMF
                     SDGKKILQQHE"
BASE COUNT          607 a          265 c          348 g          667 t
ORIGIN      
        1 tatttttgta aagtaaggct taactttttc tgttgttttg taaactatat tttatatttt
       61 ctatgagtaa atctaattcg gtgtaatacg ttgttttcca gaattctcgc cattcttgcg
      121 tttttgaaat tgaattgaat gccggatttg taatgactgt agaatattgc agtttatgtg
      181 tagagagtaa atgtttacgt aaatagatta aagccgaatc ggtctgttgt tgtatggcat
      241 atatacttgc aatataatag taactcatgg gagtatgtgc gagtgaaaag cattgtaaag
      301 cctgtgtata cattgcagta gcaaaatatg ttttgccaca taattctgca tattccggtt
      361 gtgaattgtg cggagaaata tatgtaaata cagaatcgta gtagccataa tcataagcaa
      421 cttgagcatg caataggtga ggggtttgag aaaaagcggg ttgtaatcct attacaatca
      481 aaattatata tataaatttt ttactcatgt tgttgtaata ttttttttcc gtcagagaac
      541 atatgaataa atcctactaa tgtacgtgga tgaatattac aatcggccca atattcataa
      601 acatcacaaa cataataata aactccgtca tcaagaggtt tctttgtctt accattgaaa
      661 cctttccaca aaattgaagg attttttgtt tcaaatacca gatttcccca actgtcatat
      721 attttcatat caacatattc tacaaatctg tttacggtag gatgatatac atcattttgc
      781 ccatcggcgt ttggtgtaaa aatatttggt aatatataga gaggacaatt atacaagcag
      841 gtatcattcc gtatagttga ttcattaccg gcagaatcaa tacaagtcat ataataacaa
      901 cctgccatag aacgaaccgt atctgtaaac gtatgaatga attgtgtttg tagttttgaa
      961 agacttgcta atagcgtata ggaatttgtt ttgcaatgtt tgtaatatat atttacagtt
     1021 tctatttcat cggttgtgtc tatagtccat gaaatagtac gagatagtgc attgcaattt
     1081 tggtttataa caaattttgg tgctttggga ggcaccgtgt cggtaggata tgtacatgct
     1141 atttgcgaaa aattatatac ccgttgtggt aattgagcat tagtaaaata tccgcttgtt
     1201 tttacataat aacagtattc ttgtaagttt acaagaccgc tgtcaataaa ttcttgtccg
     1261 ttgcatatgc ctatatattt gaatatatca gaatttaatt cttttcgata tacataaata
     1321 gaatcgtttt gccagccaac ttgtgcattt actcgtaatg ttattttttt attttgagga
     1381 ataagtgtaa caaaaggtga agagcaaatt ggcggattcc caatgagaat aggtgaaccg
     1441 cttgccaaag aatataattc tattttatat gaaaatacag aatcggttgt atttataccc
     1501 gtgtcaataa aggtagtatc atataacccc tgaatagttc caatttcttg taaattggta
     1561 ccaaagtatc cgaatgaacg atatatttta tattgatagg gtttaggata ggtaatagaa
     1621 tcaaaatcac ggggcaatga ccaagctata aaaatttctc cctgattttt atgggttttt
     1681 tgtacactca catttgtaat tacatgaata gtaggtttta gttctacaca tatttctggc
     1741 gatatgtatg atgggaaatt atcggagtaa tgggatagaa ttctgtagca atacgtaaaa
     1801 ccaggactta accctatgcc gttattagaa tcatatagat tgtgttgaga accgggaaca
     1861 gtgtgtatat acgaatatga tggttgt
//
LOCUS       DAOTOW010000476         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47662_length_1885_cov_3.800546, whole genome shotgun sequence.
ACCESSION   DAOTOW010000476 DAOTOW010000000
VERSION     DAOTOW010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47662_length_1885_cov_3.800546"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1885)
                     /locus_tag="P5243_08325"
     CDS             complement(<1..>1885)
                     /locus_tag="P5243_08325"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRS19496.1"
                     /translation="TATNASARTAYGATQSLNKGLTVAFRSGAVMGLVVVGFGLLDIA
                     AWYWVLNYIYDGNVFGMGVSVAREVGLGDIWNSSFVNEGAWMHAKAVHITTTMLTFGM
                     GASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAG
                     MGADLYESYCGSILATAALGAAISLSPDGSFDDKKILAPMIVAAIGIIFSIVGIFMVR
                     TKESASAKSLLNSLLIGTAGSSVLILIGIAVMASVGWLPWGIFGSVVSGLIAGIVIGQ
                     GTEYYTSDGFKPTQGIAKNAQQGPATTIIDGIAVGMYSTWIPVVTIVLGIIAAYGFAG
                     GFSDFSQGVYGIGFAAVGMLSTLGITLATDAFGPIADNAGGNAEMAHLPKEVRERTDA
                     LDMLGNTTAATGKGFAIGSAALTAMALLVAYMEEIRLWLGKLPAFGQAFLEKTGAESL
                     SKASIQDFVTFYHLDLFNPLLLAGLFIGAMMAFVFCAMTMKAVGRAAGSMVDEVRRQF
                     KEIPGIMEGTGTPDYARCVAISTKGAQREMLVPSLLAIAVPLVVGILLGVAGVIGLLM
                     GGLVTGFTLAVMLNNSGGAWDNAKKFVEKGNYGGKGSDTHKAAVTGDTVGDPFKDTSG
                     PSL"
BASE COUNT          637 a          461 c          274 g          513 t
ORIGIN      
        1 ttaagagatg gacctgaagt atctttgaat gggtcaccta cagtgtcacc agttacagca
       61 gctttatgag tatcagatcc ttttccgcca taattacctt tttcaacaaa tttcttagcg
      121 ttatcccaag ctccacctga attgtttaac attacagcaa gagtaaatcc tgtaaccaaa
      181 ccacccataa gcaaacctat tacccctgct acaccaagta aaatacctac taccaaagga
      241 acagctattg ctaataatga tggtaccaac atttcgcgct gagcaccttt tgttgaaata
      301 gcaacacaac gagcataatc aggagtacct gttccttcca taattcccgg aatttcttta
      361 aactgacgac gtacttcgtc aaccatagaa ccagcagctc tacctacagc tttcatagtc
      421 atagcacaga aaacaaaagc catcatagca cctataaata aacctgccaa taatagtgga
      481 ttaaataaat ctaaatgata gaatgttacg aaatcttgaa tagacgcttt tgaaagagac
      541 tctgcccctg ttttttcaag aaatgcttga ccaaatgccg gcaacttacc taaccataaa
      601 cgaatctctt ccatataagc aaccaacaat gccatagctg taagagctgc agaacctatt
      661 gcaaaccctt tacctgtagc tgcggttgta tttccaagca tatcaagtgc atcagtacgt
      721 tcacgaactt ctttaggtaa gtgagccatt tcagcattac ctccggcatt atcggcaata
      781 ggaccaaaag catcggtagc caaagtaata cctaatgtag aaagcatacc aactgccgca
      841 aaaccaatac cgtaaacacc ttgagaaaaa tcagaaaatc ctcctgcaaa accatatgcg
      901 gcaattatac ctaatacaat agttactaca ggaatccagg tagaatacat acccacagct
      961 ataccatcta taatagtagt tgcaggacct tgttgcgcat tttttgcaat accttgtgta
     1021 ggtttaaatc catcagacgt ataatattct gtaccttgtc ctattacaat acctgcaatt
     1081 aatccggaaa caactgatcc aaaaataccc catggtaacc aaccaacaga agccattaca
     1141 gctataccaa tcaatattaa tacagaactt cccgcagttc ctattaataa tgaatttaat
     1201 aaacttttag ctgaagctga ttctttggta cgtaccataa aaataccaac aattgaaaat
     1261 ataattccaa tagctgctac tatcatagga gctaatattt ttttatcatc aaaactacca
     1321 tcgggtgaca atgatattgc cgctcctaat gcagcagtag ctaatataga gccacaataa
     1381 ctttcatata aatcagctcc catacctgct acgtcaccta cgttatcacc tacgttatcg
     1441 gcaatagttg caggatttcg cggatcatct tcagggattc ctgcttcaac tttacccact
     1501 aaatcggctc ccacatctgc tgcttttgta taaataccac cacctacacg agcaaataag
     1561 gcttgtgtag aagcacccat accaaatgtt aacatagttg tagttatatg aactgctttt
     1621 gcatgcatcc aagctccttc atttacaaat gaagaattcc atatgtcacc taaaccaact
     1681 tcacgcgcaa cagacacgcc catgccaaac acgttaccat catatatata attaagtacc
     1741 caataccatg cagcaatatc taataatcca aaacctacaa caactaaccc cattactgca
     1801 ccacttctaa atgctacggt caatccttta tttaatgatt gagttgcacc atatgcagta
     1861 cgagctgatg cgttggtagc tgttt
//
LOCUS       DAOTOW010000477         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47781_length_1882_cov_3.218938, whole genome shotgun sequence.
ACCESSION   DAOTOW010000477 DAOTOW010000000
VERSION     DAOTOW010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47781_length_1882_cov_3.218938"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1666
                     /locus_tag="P5243_08330"
                     /pseudo
     CDS             56..1666
                     /locus_tag="P5243_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            1678..>1882
                     /locus_tag="P5243_08335"
     CDS             1678..>1882
                     /locus_tag="P5243_08335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRS19497.1"
                     /translation="MFLYDFTQLTSSIHSSLVESFGEGALTTTIELAIAGVAYLIFFA
                     VLGLFLVYLERKVCAFIQNRLGPM"
BASE COUNT          628 a          337 c          359 g          558 t
ORIGIN      
        1 acaagattca ttgacaacca aaattgacca cgtgtcaaac tttatataat acgatatgac
       61 aaatcaagaa ttacaacaaa gaatattgga attggctccc caagctgttt gtaccgaaaa
      121 taaacagtat ctgatagcac aaattcctgc tcaacaactg catagtgttg ccaaacaact
      181 caaggaatca aatgatacca aatttgatta cttgtttagc cttaccggag ttgatgcaaa
      241 tcctgcatta ggtgtggttt atcacttaga atctacagaa ttagggcatt gtgttgtatt
      301 gcaaacaatg accgaaaatc gtgaaaaacc agttttagat tcagtatgtg atatatggaa
      361 aggtgccgaa ttacaagaac gcgaagtgtt tgattttttt ggtattacat tcaacaatca
      421 ccccgatatg cgtcgtttgt tcatggacga agattggcag ggatatccat tgcgtaaaga
      481 ctacgttgat acggtaaaca ttatagattt aattaaataa cattcttcaa taatatatac
      541 aatggctgaa acagtagcac aacaaacaga cctgatagac gaagaatatt ttattaacat
      601 ggggcctcaa cacccttcaa cacacggtgt gttgcgtttg ttggtaaatc ttcacggaga
      661 aattgtaaac gaccttaaac catattgcgg atatattcat cgcggtatag aaaaaatgtg
      721 cgaacgcaat acctatccgc aaattataca tcttaccgac cgattggatt acctgtcggc
      781 acatcaaaac aacgaagcgg tgtgtatgtt ggtagaaaac gcgttggaag tagaaatacc
      841 ggaacgtgta aaatatattc gcactattat agatgaactt tcgcgcttag cttcgcacca
      901 attgtggtgg gctgcatttg gtatggactt aggtgcactt actacctttt tctatggttt
      961 gcgcgatcgt gaaaaaattc tcgatatttt tgaagaaact accggtagtc gtttgattca
     1021 aagttactat tgccccggtg gattgatgta tgatattcac ccgaatttcc aaaaacgagt
     1081 aaaagaattt ctagaatatt ttaaaaagaa attgcctgaa tacgatgaat tattgtcggg
     1141 taacgtaatt tttataaacc gaaccaaaga tattggatat ctatcaaaag aagatgccat
     1201 atcatttggc gtaaccggac catcgggacg aggttctgga tttgcatgtg atatccgcaa
     1261 aaaatatccg tacaatgtgt acgataaagt acaattcaac gaaattataa gtaccgaatg
     1321 cgattcgctt gcacgataca aagttcgtat tgccgaaatg tgggaatcta tttcgattat
     1381 agaacaactc atcgacaata ttcccgaagg ggactatcag gtaaaaatga aaccaattat
     1441 taaattaccc gaaggagaat attatcaacg aatagaatca aaccgaggag aatttggggt
     1501 atatgttcaa agtcgtggcg aaaaaaatcc atatcgtata aaattccgtt caccgaactt
     1561 cgcaaatctt tcggcactca atcacatgtc taaaaaccaa aaaattgccg atttagttgc
     1621 aattatgggt tcgctcgatt tagttattcc agatattgac agataaaatt atatagtatg
     1681 tttctttacg attttacaca attaacatca tctattcaca gctcattggt tgagtcgttt
     1741 ggcgaaggag cccttactac caccattgaa ttagctattg ccggggttgc ataccttata
     1801 ttttttgcag tacttggctt atttttggtg tatttagaac gaaaagtgtg tgcatttatt
     1861 caaaaccgtt taggccctat gc
//
LOCUS       DAOTOW010000478         1880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47863_length_1880_cov_2.575342, whole genome shotgun sequence.
ACCESSION   DAOTOW010000478 DAOTOW010000000
VERSION     DAOTOW010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47863_length_1880_cov_2.575342"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1880)
                     /locus_tag="P5243_08340"
     CDS             complement(<1..>1880)
                     /locus_tag="P5243_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS19498.1"
                     /translation="LQAQEDLSVYSTGKLKKLGIKALQDKEPYKAIRYFEEYLKIKED
                     PAIMYQLAESYRFARNYPFAQYWYDKAYKTSPKKNGKALYYYATMLKTSQMYDEAETQ
                     FKTFKKEYVDYDDSEIYQKITKNQIISCDSATSMIKKPVQARITHLGVSINTEAMEYM
                     PILLSNTSMVFAVEPFDTVSSSDAIKAPALYGANKSGFNWVNSGEWDLKAENSSPIGM
                     YHGAFSPDFQRFYFTKCEKVKKMGVICKIYMSKNSFGVWQAPEELPEIVNGKGFSNIQ
                     VAVGNESKKNDEVLYFVSNRPGGQGGLDIWFTIFMPKKNEWREPKNCGNKMNTAGDEM
                     SPYYDVVSRTMYYSSTGHAGLGEFDIFKTNGELGKWTPAENMGYPINSPYDDYYFVLA
                     SDAKSGIFASNRPGGVVAGHETCCDDLYEVSFENVFEIPVTGRVFEVEDKEIKKLINK
                     NFQTEGMEAKKDTDEIRFIPGSTVSLFIGNTNDKVFVSKTETNEKGAYLFKVEPGKNY
                     VLQFENVKTGRAFIPFTTKGIDKPDTIKIRDYGINFISKQSMVMKNIYYEYGKYKLTK
                     EQKAAIDSSIISLMKQAPEIVIELSSHTDNQGSEEFNLKLSQKRANEIVAYMIKQGIE
                     PN"
BASE COUNT          560 a          323 c          279 g          718 t
ORIGIN      
        1 atttggttca attccttgtt ttatcatata tgcaacaatt tcattagcac gtttttgtga
       61 taattttagg ttaaactctt cactaccttg attatcagta tgactgctta attcaattac
      121 aatttctgga gcttgtttca taagtgaaat tatagaagaa tcaatagcag ctttttgttc
      181 tttggtaagt ttgtatttac cgtattcata gtaaatattt ttcattacca ttgattgttt
      241 acttatgaag ttaataccat aatcacgaat tttaatagtg tcgggtttgt caataccttt
      301 agttgtgaaa gggatgaaag cacgtccggt tttaacgttt tcaaattgta gtacataatt
      361 tttgcccggc tctactttaa acaaatatgc acctttttcg tttgtttctg tttttgacac
      421 aaatacttta tcattggtat tgccaataaa tagtgaaact gtactaccgg gaataaaacg
      481 aatttcatcg gtatcttttt tagcttccat accttctgtt tggaagtttt tattgataag
      541 ttttttaatt tctttatctt caacttcaaa aactctacct gtaactggaa tttcaaatac
      601 attttcaaaa cttacttcgt ataaatcatc acaacaagtt tcatgacctg caactacgcc
      661 accgggacga tttgaggcga aaattcctga ttttgcatca gaagcaagta caaaataata
      721 gtcatcatag ggtgagttta ttggatatcc catattttct gcaggagtcc atttaccaag
      781 ttctccattt gttttaaata tgtcaaactc acctaaacct gcatggccgg tagaactata
      841 atacatagta cgtgatacaa catcataata tggtgacatt tcatcaccgg cagtattcat
      901 tttatttcca caatttttag gttcacgcca ttcgttcttt ttaggcataa atatagtaaa
      961 ccatatatct aaaccacctt gtccgccagg tctgtttgaa acaaaataga gaacttcatc
     1021 attttttttg gattcattac ctacggcaac ctgaatgttt gaaaatcctt taccgtttac
     1081 tatttcaggt aattcttcgg gagcttgcca tacaccaaat gaattttttg acatatagat
     1141 tttacaaatt actcccattt ttttaacttt ttcacatttt gtaaaataaa aacgttgaaa
     1201 atcaggagaa aatgcgccat gatacatacc tataggcgag gaattttcag cttttaaatc
     1261 ccattcacca gaatttaccc aattaaaacc gcttttgttt gcaccatata atgcaggagc
     1321 ttttattgca tcgcttgaac ttacagtatc aaaaggttca actgcaaaaa ccattgatgt
     1381 atttgataat aaaataggca tatattccat tgcctcagta tttatagaaa ctcctaaatg
     1441 agtgatacga gcttgaacag gtttttttat catgcttgtg gcagaatcgc atgaaatgat
     1501 ttgatttttt gtgatttttt gataaatctc agaatcatcg taatcaacat attctttttt
     1561 gaatgttttg aattgcgttt cggcttcatc atacatttgg cttgttttaa gcattgtagc
     1621 atagtagtat aaagctttac cattcttttt tggacttgtt ttatatgctt tgtcatacca
     1681 atattgtgca aacggataat tacgtgcaaa acgataactt tctgctaatt ggtacatgat
     1741 agcaggatct tctttaattt ttaaatattc ttcaaagtat cgtattgctt tatacggttc
     1801 tttatcttga agagctttta ttcctaattt ttttaatttt ccggtgctat ataccgataa
     1861 atcttcttga gcttgtagtg
//
LOCUS       DAOTOW010000479         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_47959_length_1877_cov_3.233809, whole genome shotgun sequence.
ACCESSION   DAOTOW010000479 DAOTOW010000000
VERSION     DAOTOW010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47959_length_1877_cov_3.233809"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="P5243_08345"
     CDS             complement(<1..1101)
                     /locus_tag="P5243_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19499.1"
                     /translation="MKKLIFTLAFIISFLGIYAQDADFISTANSIKIVALYSNTLNQI
                     NKIEAKEEKVIQIDKSITQEDFDKICAKLKWIKKLSFDYDNPFITNIMSLQNLTSLEL
                     LKMRNISSSEKQAIDLSPLAKLTLLKEIDFLGTAVTNTDAFNSLINLEKINFTDSKIA
                     SIQFLSNTPLVKELILAGNNHTFPNYEPIQNLKKLRVLDIQKNSQATDRNVAVLQNLQ
                     ELRELDISDCKGITQLQFIEQCTKLVEFSAANCTNIVNYSALQNLILLKKINLSGSSV
                     TDISFVSNKSDLKDLQISKTAISDITSLNMCASIERLDISYTQVTDITTISRLWKLKR
                     LNIAHTKIASLDALGANFELTDLDASFTQVSDL"
     gene            complement(1261..1461)
                     /locus_tag="P5243_08350"
     CDS             complement(1261..1461)
                     /locus_tag="P5243_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19500.1"
                     /translation="MVYYSEGVDENGSPLAEWDGYYNGELLQSGTYIWKIQASFVNGT
                     NWVGEDKNISNKSTFGNVMLIR"
     gene            complement(1463..>1877)
                     /locus_tag="P5243_08355"
     CDS             complement(1463..>1877)
                     /locus_tag="P5243_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19501.1"
                     /translation="VTDGPDADFITQNPGEEGLIEFTNTSKKQIATSNDSIYEFHVDY
                     YWNYGRITDETVLNNAPKFRQQYNFGTHTISLTAVNEAGCRNSISKDIFVDMTYGLYV
                     PSAFAPENPAYGVRKFKPRGFNMTSFAISIFDAWG"
BASE COUNT          609 a          261 c          281 g          726 t
ORIGIN      
        1 taaatcgctt acttgagtaa atgatgcatc taaatcggtt aattcaaaat tagctcctaa
       61 tgcatctaat gatgcaattt ttgtgtgtgc aatatttaaa cgttttagtt tccaaagtct
      121 cgaaatagtt gttatatcag ttacttgtgt atatgaaata tctaaacgtt ctatagaggc
      181 acacatgttg agtgatgtaa tgtctgaaat tgcagtttta gaaatttgta aatcttttaa
      241 atcagattta ttcgatacaa aactgatatc agttacagaa cttcccgaaa gattaatttt
      301 tttgagtaaa attaaatttt gtaatgcaga atagtttaca atgtttgtac aatttgcagc
      361 actaaattca actaattttg tacattgttc tataaattgt aattgagtta tacctttaca
      421 atcagaaata tctaattctc gtagttcttg aagattttgt aatacagcta catttctatc
      481 ggtagcttgt gaattttttt gtatatcgag tactcgcaat ttttttaaat tttgtattgg
      541 ttcatagtta ggaaatgtat gattatttcc tgctaatatt agttctttaa ctaaaggagt
      601 atttgataaa aattgaattg aagcaatttt gctgtcagta aagtttattt tttcaaggtt
      661 tattagacta ttaaaagcat cagtgtttgt aactgctgtt cctaaaaagt caatttcttt
      721 gagtaatgta agttttgcca aaggacttaa atcaatagct tgtttttcag aagatgaaat
      781 atttcgcatt ttgagtaatt caagtgatgt aagattttga agtgacataa tgttggtaat
      841 aaacggattg tcgtagtcaa aacttaattt ttttatccat tttaattttg cacagatttt
      901 atcaaaatct tcttgtgtaa ttgatttgtc aatttgaata actttttctt cttttgcttc
      961 aatcttatta atttgattga gagtatttga gtagagtgca acaattttga tagaatttgc
     1021 tgtgcttata aagtctgcat cttgggcata tatgccaagg aatgaaatta taaatgcgag
     1081 ggtaaaaatc agttttttca tacgcttcaa tttttaaatg ttagtataaa gcttacatct
     1141 aacttgtacg aaaaacaaat aaaaaaggat tattaatttt tataataatc cttcttaaaa
     1201 atttgtaagt aattgaaaat ctttatttaa aaaaataaaa ttgagtgttt ttactatatt
     1261 ttatcgtatt aacattacat ttccaaatgt ggatttattt gatatatttt tgtcttcacc
     1321 cacccaatta gtaccattta caaacgatgc ttgaattttc caaatataag tacccgattg
     1381 taataattca ccattataat atccatccca ttcggcaagt ggacttccat tttcatcaac
     1441 tccttctgaa tagtatacca agttaccccc atgcatcaaa tatagaaata gcgaaacttg
     1501 tcatgttaaa tcctcgaggt ttgaatttgc gaacaccata tgccggattt tcaggagcaa
     1561 atgcagatgg tacgtagagt ccgtaagtca tatctacaaa tatatctttg cttatactgt
     1621 ttctacaacc ggcttcgttt accgcagtta aactaatagt atgagtacca aagttatatt
     1681 gttgacggaa ttttggagca ttgtttaata cggtttcatc tgttattctt ccgtagttcc
     1741 aataataatc aacatggaat tcatagatag aatcattact tgttgctatt tgctttttag
     1801 atgtattagt aaattcaatt aatccttctt ctcccggatt ttgagttata aagtcagcat
     1861 caggaccatc tgtaaca
//
LOCUS       DAOTOW010000480         1874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_48064_length_1874_cov_3.104453, whole genome shotgun sequence.
ACCESSION   DAOTOW010000480 DAOTOW010000000
VERSION     DAOTOW010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48064_length_1874_cov_3.104453"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1874
                     /locus_tag="P5243_08360"
     CDS             <1..>1874
                     /locus_tag="P5243_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRS19502.1"
                     /translation="KAASARTITVMFQQLAADYAVYKTFTYNLTTTAQTFTETFTLTG
                     TVASASKIAFCVGNNAACVSIDKVSFGYGTTSGGGTVDPPVSNGQGAFFTDVYTNLFK
                     EVLGKTDTEINTKVTNAFQQIFYGTSTQKLYYEVGTDMAYILDVNNNDVRTEGMSYGM
                     MICVQLNKQAEFNKLWKWAKTYMQYGTSSNYSGYFAWQCNTDGSIKGNGPASDGEAYF
                     ITALFFAANRWGNGTGIYNYAAEAQDILVRVMNKTGAGSVYNLFNSTSKLITFVPYGD
                     SYNFTDPSYNLPAFWELWARWSTSNTTFWAQTPEASRKLLRDASHPTSGLTTDYSNFD
                     GTPKEVSYNTDADRFMYDAWRTAMNIGMDYHWFRTDSTNQRAIVTRYLTFFKNQGTNY
                     KNHYDWNGANPGGDHSTGLVACNAAACFAVSDNTLRTPFLTEFWNIAIPTGTYRYYDG
                     MLYMLALLHCSGNFKIWKPTCTPPSAPTVTTPVTYCQGATATALTATGTSLNWYTVAT
                     GGTASTTAPTPSTATAGNTTYYVSQTVSGCESTRASIVVTITALPSAPTVTTPVIYNQ
                     GAVSTALTATGTSLKWYTVATGGTASTTAPTPSTTTVGNTTYYVSQTVSGCESPRALI
                     "
BASE COUNT          598 a          336 c          374 g          566 t
ORIGIN      
        1 aaaagccgca tcggctcgta ctattacggt tatgtttcag cagttggctg ccgattatgc
       61 cgtgtataaa acatttacct acaatcttac cacgacagca caaacattta ccgaaacatt
      121 tacacttact ggcactgtag catcggcttc aaaaattgct ttctgtgttg gtaataatgc
      181 cgcttgtgta agtattgata aagtttcgtt tggatatggt acaacaagtg gaggtggcac
      241 ggttgatcct ccggttagta atggacaagg agcttttttt acagatgtat atactaattt
      301 atttaaagaa gtattaggta aaactgatac ggaaataaat actaaagtaa caaatgcttt
      361 tcaacaaata ttttatggaa cttcaactca aaaattatat tatgaagttg gaactgatat
      421 ggcatatatt cttgatgtaa ataataatga tgtgcgtaca gaaggaatgt cgtatggtat
      481 gatgatttgt gttcaattaa ataaacaagc agagtttaat aaattatgga aatgggctaa
      541 aacatatatg caatacggta cttcaagtaa ttacagtggt tattttgcat ggcagtgcaa
      601 tactgatggt tctattaaag gaaatggtcc ggcatcagat ggtgaagcat attttataac
      661 agctttattt tttgcagcca acagatgggg aaatggaaca ggtatatata attatgctgc
      721 agaagctcaa gatatattag ttagagtaat gaataagacg ggtgccggta gtgtttataa
      781 tctttttaat tcaacatcaa aattaataac gtttgtaccg tacggtgatt cgtataattt
      841 cactgaccct tcgtataatt tgcctgcatt ttgggaatta tgggctcgat ggagtacaag
      901 taatacaacc ttttgggctc aaacgccaga agcatctcgt aaattattac gtgatgcttc
      961 gcatccaaca tcaggattga caactgatta ttctaatttt gatggaaccc ctaaagaagt
     1021 atcgtataat actgacgcag atagatttat gtacgatgct tggagaacag ctatgaatat
     1081 tggtatggac tatcattggt ttagaactga ttcaacaaat caaagagcaa tcgttactcg
     1141 atatttaaca ttttttaaaa atcaaggtac gaattataaa aatcattatg attggaatgg
     1201 agcaaatccg ggtggagacc attctacagg tttagttgcc tgtaatgcag cagcttgttt
     1261 tgcagtaagt gataatacgt tacgtacgcc atttttaact gaattttgga atattgctat
     1321 tcctactggc acatacagat attatgatgg aatgctctat atgcttgcat tgttacactg
     1381 ttcgggtaat tttaaaattt ggaaacctac gtgtactcca ccatcagcac ctacagttac
     1441 tactcctgtt acgtattgtc aaggagcaac agctacagca ctcactgcta caggcacatc
     1501 gcttaattgg tacacagtgg caacaggagg tacggcaagt actacggctc ctacgccaag
     1561 tactgcaact gcaggcaata ccacatacta tgtaagtcaa acagtatcgg gctgcgaaag
     1621 tacgcgcgca agtattgtag taacaattac tgctttacca tcagctccta cggttactac
     1681 tcctgttatt tataatcaag gagcagtatc cacagctctt actgctacag gcacatcgct
     1741 caaatggtac acagtagcaa caggtggtac ggcaagtaca acagctccta caccaagcac
     1801 aacaacagtt ggtaatacca catactatgt gagtcaaacg gtatcgggtt gcgaaagtcc
     1861 tcgtgctctt attc
//
LOCUS       DAOTOW010000481         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_48511_length_1863_cov_2.839049, whole genome shotgun sequence.
ACCESSION   DAOTOW010000481 DAOTOW010000000
VERSION     DAOTOW010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48511_length_1863_cov_2.839049"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="P5243_08365"
     CDS             complement(<1..824)
                     /locus_tag="P5243_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRS19503.1"
                     /translation="MKFDSQLKLQEIIAEYEALVSKKNVLSSEYNGIYQRYKNPILTA
                     QHVPIHWKFDLDLQRNPLLVQRIGVNAVFNAGAMKYNGKYILCARVEGWDRKSYFAIA
                     ESPNGIDNFRFWDEPIRIPQLSNPDTNVYDMRLTKHDDGFIYGIFCTERKDPNAPKGD
                     TSSAIANAGIVRTKDLKQWERLPDLISNTGQQRNVVLHPHFVDGKYMVYTRPQDGFID
                     VGSGGGIGLGYITDITNPVVEQEHIIHNKQYHTIYELKNGLGPAPIKTNKGWLHLA"
     gene            complement(926..>1863)
                     /locus_tag="P5243_08370"
     CDS             complement(926..>1863)
                     /locus_tag="P5243_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRS19504.1"
                     /translation="AGSNQIMWRYSKNPIVNWNPIPTASRVYNSAVVPFNGAFAGVFR
                     GDQMNGRATLFSGFSKDGVTIELNPEPIAWVDEQGNPKPTSYSYDPRVVEIDGIYYVT
                     WCDDMKGPSIGLGMTKDFKTFTRLPNPLMPYNRNGVLFPRKINGKYFLLSRPSDTGHT
                     PFGNIFISESPDMVHWGNHRFVMGNGGQGWWQGTKIGAGPVPIETNEGWLLFYHGVSG
                     TCNGFVYSIGAVLLDINDPGKVLYRCGNYLLTPEKEYETVGFVPNVAFPCANLYDAAT
                     GKIAIYYGAADTYTAVAFCQVDEVIDYIKKHNEKF"
BASE COUNT          612 a          383 c          272 g          596 t
ORIGIN      
        1 tgagctaaat gcaaccagcc tttattagtt ttaataggtg caggtccaag tccgtttttt
       61 aattcataaa tagtatgata ttgtttatta tgtatgatat gttcctgttc tactactgga
      121 ttggtaatat ctgtaatata tcccaatcct atacctccac ctgagccaac gtcaataaaa
      181 ccatcttgtg gacgagtata gaccatatat tttccatcta caaaatgagg atgtaacact
      241 acatttcgtt gttgcccggt atttgaaata agatcaggta atcgttccca ttgttttaaa
      301 tctttggttc taacgattcc tgcatttgca atagcagaac ttgtatcgcc tttaggagca
      361 ttagggtcct tgcgttcggt acaaaaaatt ccatatataa acccatcgtc gtgttttgtt
      421 aatcgcatat catacacatt agtatcagga tttgataatt gaggtatacg aataggttca
      481 tcccaaaatc taaaattatc aattccatta gggctttctg caatagcaaa atatgatttt
      541 ctgtcccacc cttctacacg ggcacacaaa atatattttc cattgtattt catagcaccg
      601 gcattgaaaa cagcattcac tcctatgcgt tgaacgagca atggattacg ttgtaaatct
      661 aaatcaaact tccaatgaat aggaacatgc tgtgctgtta atataggatt cttatatcgt
      721 tgatatattc cattatattc cgaagataaa acattttttt tactaactaa cgcttcatat
      781 tcagctataa tttcttgtaa ttttagttgt gaatcaaatt tcatgtatta aaaaatatta
      841 attaaaaaaa gaaaaaaagt atagagataa caacgcgtgt atcataccat acacatcata
      901 accactatac ttaaaaatat gagaattaaa atttctcatt atgtttttta atataatcta
      961 taacttcgtc aacttgacaa aaagcaacag ctgtatacgt atcggcagct ccatagtata
     1021 tagctatttt tcctgttgca gcatcataca gattagcaca aggaaaagca acattaggaa
     1081 caaacccaac cgtttcatat tctttttcgg gggtaagcaa atagttacca caacgataca
     1141 atactttacc cggatcatta atgtctaata atacagcacc tatactatat acaaatccat
     1201 tgcatgtacc cgaaacaccg tggtaaaaca acaaccaccc ctcattggtt tcaataggaa
     1261 caggccccgc acctattttg gtaccttgcc accaaccttg acctccgtta cccataacaa
     1321 atctatgatt accccaatga accatatcag gactttcact aataaaaata ttaccaaaag
     1381 gagtatgacc ggtatcactc ggacggctca ataaaaagta ttttccattt atttttcgtg
     1441 gaaataatac accatttcta ttataaggca taagtggatt tggcaaacgt gtaaacgttt
     1501 taaaatcttt tgtcatacca agccctattg atggaccttt catatcgtcg caccatgtaa
     1561 cataatatat tccatcaatt tctacaacac gaggatcata cgaatagctt gttggcttag
     1621 gatttccctg ttcatcaacc caagcaattg gttcgggatt tagctctata gttacaccat
     1681 ctttactaaa ccctgaaaat aatgttgcac gaccgttcat ttgatcgcca cgaaatacac
     1741 ctgcaaatgc gccattaaat ggaacaacag cactattata cactcgcgat gctgtaggta
     1801 taggattcca attcacaatg ggatttttac tatatctcca cattatttga ttacttcctg
     1861 ccg
//
LOCUS       DAOTOW010000482         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_48562_length_1861_cov_3.355482, whole genome shotgun sequence.
ACCESSION   DAOTOW010000482 DAOTOW010000000
VERSION     DAOTOW010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48562_length_1861_cov_3.355482"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1861
                     /locus_tag="P5243_08375"
     CDS             <1..>1861
                     /locus_tag="P5243_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRS19505.1"
                     /translation="NGIDYVFNSQFCTIFPRMYSREPLHIKEYKDWAGIKDGVNTRKV
                     FYEKERQSYNVPTFGQNLKFFFSYQIGHMYIRYFMWNFAGRQNDVQSHGGIINGNWIS
                     GIPFIDNQLVGDQTFISDKAKNNKTRNTYYLLPFALGVLGFLFHILQRKRDSFVVVLL
                     FVLTGLAIVVFLNQTPYQPRERDYAYAGSFYAFSIWIGLSVLAFYHYSKKYLKHGGIS
                     ATIAFLIAAPTPVILAKENWDDHDRSGRYTARDFAYNYLMSCAPNALIVTAGDNDTFP
                     LWYAQEVEGIRTDVRVLCTPLLATDWYSQQMMRRTYESAPLPLKLTEKQVAKGIRDIV
                     RVQESSFTQGKYVWLKDAMEIVRDDEMKIKLYGDSYNYMPASLLKFPVDKNQVLSQRI
                     VSLKDSLLIAPEIRLEVKDKSIYKNKLIIYDVLDGYQWDRPLYFTNQMSAEELGLEKY
                     VRYDGFTFKFVPIESANPRIPHTDTDVMYNNIMNVYKWGGMGDSTVHMCHFNERTTRV
                     IGIRSMFNQLALNLANEGKKDSARAVLAKLKTIMPDWQFPYFDDNIIYTAYAYYAIGD
                     TKGAQEELSIYVENLIQEIEWFNSLQPKFAAMVQKDKEEYMRSIVQIADVVQSR"
BASE COUNT          648 a          301 c          331 g          581 t
ORIGIN      
        1 aatggtatag actatgtatt taattctcaa ttttgtacca tctttccgcg tatgtatagt
       61 cgcgaacctc ttcatataaa agaatataaa gactgggcag gtataaaaga tggagtaaat
      121 acccgtaagg tgttttatga aaaagaacgt caatcatata atgtacctac atttggtcaa
      181 aatttaaaat tctttttctc atatcaaatc ggacatatgt atattcgcta ttttatgtgg
      241 aattttgcag gtcgccaaaa cgatgtacaa agtcatgggg gcattataaa cgggaattgg
      301 ataagcggca ttccttttat tgacaatcaa ttagttggag atcaaacatt tatttcagac
      361 aaggctaaaa acaataaaac tcgaaacacc tattatttat taccatttgc acttggtgta
      421 ttaggattct tgtttcatat attacaacgt aaacgcgatt cgtttgtagt agtattatta
      481 tttgtactta ccggattggc aattgtagta ttcttaaatc aaactcctta tcaaccacgt
      541 gaacgagact atgcatatgc aggttctttt tatgccttta gtatatggat tggattaagt
      601 gtacttgcat tctaccatta ttctaaaaaa tatttaaaac atggaggtat aagtgctacc
      661 atagcattct taattgctgc tccaactcca gtaatattag ctaaagaaaa ctgggacgat
      721 catgatcgtt ccggtcgata cactgctcgt gattttgcat ataattatct catgagttgc
      781 gccccaaatg cgttaattgt aactgccggc gataatgata cattcccgct ttggtatgct
      841 caagaagtag aaggaatcag aacagatgta agagtattgt gcacaccact cttagctacc
      901 gattggtact cacaacaaat gatgcgtaga acatatgaat cggcaccact gcctttaaaa
      961 ttaacagaaa agcaagttgc taaaggtatt cgcgatatag ttcgtgtgca agaatcaagc
     1021 tttacacaag gtaaatatgt gtggctaaaa gatgcaatgg aaatagtacg tgacgatgaa
     1081 atgaaaatta aactctacgg cgattcatat aattatatgc ctgcaagttt acttaaattt
     1141 ccagttgaca aaaatcaggt attatcacaa cgtatagttt cgctcaaaga ttcattactt
     1201 atagcacccg aaataagatt agaagtaaaa gataaatcta tatacaaaaa taaattaatt
     1261 atttatgatg tattagacgg gtatcaatgg gatagacctc tctattttac aaatcaaatg
     1321 tcggcagaag aattaggtct tgaaaaatat gttcgctacg atggatttac attcaaattt
     1381 gttccaatag aaagtgcaaa tcctcgtata cctcatactg atacagatgt aatgtacaat
     1441 aacataatga atgtatataa atggggtggc atgggcgaca gtactgttca catgtgtcat
     1501 tttaatgaac gcacaacacg tgttataggt attcgttcta tgttcaatca actagctctc
     1561 aatcttgcaa acgaaggcaa aaaagattca gcacgtgctg tattagcaaa attaaaaacc
     1621 ataatgcccg attggcaatt cccatatttt gatgataata ttatatacac agcatatgct
     1681 tattatgcaa ttggcgatac caaaggtgct caagaagaat taagtatata tgtagaaaat
     1741 ttaattcaag agatagaatg gtttaattca ttacagccta aatttgctgc tatggtacaa
     1801 aaagacaaag aagaatacat gcgttctatt gttcaaattg cagatgttgt tcaaagtaga
     1861 g
//
LOCUS       DAOTOW010000483         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_48601_length_1860_cov_3.349584, whole genome shotgun sequence.
ACCESSION   DAOTOW010000483 DAOTOW010000000
VERSION     DAOTOW010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48601_length_1860_cov_3.349584"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="P5243_08380"
     CDS             complement(<1..223)
                     /locus_tag="P5243_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRS19506.1"
                     /translation="MDQKIIDKAKQWLTGNYDEKTKKEVQYLLDNNYKELENAFYQNL
                     EFGTGGLRGVIGVGTNKMNIYTVGMATQGF"
     gene            complement(282..1640)
                     /locus_tag="P5243_08385"
     CDS             complement(282..1640)
                     /locus_tag="P5243_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRS19507.1"
                     /translation="MLNWNSLLSHKRLGIDEALNTSQDAARSQFQRDYDRIIFSSPFR
                     RLQNKTQVFPLPGSVFVHNRLTHSLEVASVGRSLGNIIANGLLEKQVDVPQSLLDGIG
                     SIVSAACLAHDLGNPPFGHSGEKAISRYFTEIDTNNIRELLTVSQFQDFINFEGNANA
                     FRLLTHQFSGRRKGGFALTYATLGTLLKYPCTSTDIALNQSPYEKYGVFQTELATLKH
                     VAQELGLKSISDSGTVFGRHPLVFLMEAADDICYQIVDLEDAHRLGILTSQQTKDLLF
                     AFFDPIADAKALDELHVLLHDVTDENEQIVILRSRTINKLIEDCAQVFWDKYDDIMNC
                     NFYSSLTKQLKGTSKQALREIQYIASNKIYNSREVVEIQVAGHRILSELLHEFITAAL
                     KNNSLYTQQLLRLLPTQLVYANADTYSRISSVVDYVAGMTDVYALEIYRKIKGISFSV
                     IR"
BASE COUNT          630 a          326 c          276 g          628 t
ORIGIN      
        1 caaacccttg agttgccata ccaacagtat atatattcat tttattggta cccactccta
       61 tcacaccacg caatcctcct gtaccaaact ctaaattttg ataaaatgca ttttctaatt
      121 ctttataatt attatcgagt aaatattgaa cttctttttt tgttttctcg tcataattac
      181 ctgtaagcca ttgtttagct ttgtcaatta ttttttgatc cattgttttg tataaaaaaa
      241 gtatattata ttaaattatt attgcaacaa aattacatgt tttatcgaat taccgaaaac
      301 gaaattcctt ttatttttct ataaatttca agtgcatata catcggtcat accggccaca
      361 taatctacaa cagaagatat tcgagaatac gtatcggcat tggcatatac aagctgcgtc
      421 ggtaacaatc gcaacaattg ttgagtgtat aatgaattat tttttaaggc tgccgtaata
      481 aattcatgta acaattcact caatattcta tgtcccgcaa cttgtatttc tacaacttcg
      541 cgactgttat aaattttatt tgatgctata tattgaattt cgcgcaaggc ttgttttgat
      601 gtccccttaa gttgtttggt tagtgatgaa taaaaattac aattcattat atcgtcatat
      661 ttatcccaaa atacttgagc acaatcttct ataagtttat taatggttcg cgagcgaaga
      721 attactattt gttcattttc atcggtaaca tcatgcaaca atacatgcaa ttcgtctaaa
      781 gcttttgcgt ctgcaatagg atcaaaaaaa gcaaacaaca aatctttggt ttgttgcgaa
      841 gttaaaattc caagtctatg agcatcttct aaatcaacta tttgataaca aatatcatcg
      901 gctgcttcca ttaaaaacac tagagggtga cgcccaaaca cagtacccga atcagaaatt
      961 gatttaagcc ctaactcttg agcaacatgc tttagtgttg ccaattctgt ttgaaataca
     1021 ccgtattttt catagggact ttgattgaga gctatatctg tagatgtaca cggatatttc
     1081 aacaaagtgc ctaaggtagc atacgtaaga gcaaaccctc cttttcgtcg ccccgaaaat
     1141 tgatgcgtta gcaaacgaaa cgcatttgca ttaccttcaa aatttataaa atcttgaaat
     1201 tgtgaaaccg taagtaattc tcgaatattg tttgtatcta tttctgtaaa atatcgtgat
     1261 attgcctttt cacctgaatg accaaaagga ggattgccta aatcatgagc caaacatgcc
     1321 gcagaaacta tagagcctat gccgtctaat aatgattggg gtacatctac ttgtttttct
     1381 aacaaaccat tagctataat attgccaagt gaacggccaa ccgatgctac ttcaagacta
     1441 tgggtaagac ggttatgaac aaatacacta ccgggcaatg gaaacacttg tgttttgttt
     1501 tgtaaacgac gaaacggaga tgaaaaaatt attctatcat aatcgcgttg aaactgagaa
     1561 cgtgcggcat cttgtgatgt attcaaagct tcatcaattc ctaatcgctt gtgcgataac
     1621 aaactattcc aattgagcat tatactaaat ttatgtatgc aagtatataa aaaaaatgag
     1681 aaatcacaat gcgtaaataa gattgatata cgttacaatg taaattacac taaaaaattg
     1741 aatggagtaa tgtaaaacat attaattcaa taatttttta tatttttatg cagaaattat
     1801 tttacatata acttctatgt aagaataata tattcaaata attactttag aaatgttcac
//
LOCUS       DAOTOW010000484         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_48791_length_1855_cov_3.601667, whole genome shotgun sequence.
ACCESSION   DAOTOW010000484 DAOTOW010000000
VERSION     DAOTOW010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48791_length_1855_cov_3.601667"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="P5243_08390"
     CDS             complement(<1..273)
                     /locus_tag="P5243_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRS19508.1"
                     /translation="MRNSIIISIWFICISFFSNTIYSQNQLIAKSQVIENIDAKKYYI
                     HTVQAKETAYAISRAYGISYSELLQHNPGIETGLSIGQTLKIPYTEQ"
     gene            complement(385..>1855)
                     /locus_tag="P5243_08395"
     CDS             complement(385..>1855)
                     /locus_tag="P5243_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19509.1"
                     /translation="NTKRALRNSFDCIEYVDACIDNKQYELAQTFLEVAVKKYADFEP
                     LYKKQIAIYSKLLQYEKLSSAVIDLLEVNPLELEFIKNQLQNLLFDQSQSKLQDILLE
                     KLLLKVNENPQSVEFNELLLWFYLQEKTFKKAFVIARSLDIRQQGFGEKVYDVGQIAL
                     SNDEFAIATECFSYVVNQGKDKPYYMAAVKMMLETTYKSLFMGQFPSVDAIKDIESQY
                     IKVLQDIGENQNTTDVIRNLAHIQGFYLHKYSEAIDRLQKSLLIPGISYVEKGLCSME
                     LADMYLFSDDIWSANLLYAKVALDFKNNDIGHQARFKQAQVAYYNAQFEYAQALLDIL
                     KASTSKLIANDAFELSLLISENTIMDTSTKALQYFSKADLLLYQNNVTEAYVYLDSIV
                     HNFPGHSLEDDVLMRKAIAAQKLQKFESMEDYLLQITQRFSYDVYADNAHFMLAEYYD
                     YIAKQPDKAQLHYKTLVTEYPSSYFSIQARKRYREIQKL"
BASE COUNT          625 a          292 c          234 g          704 t
ORIGIN      
        1 ttgttcagta taaggaattt ttaatgtttg tcctattgat aagcctgttt caattccggg
       61 attatgttga agtaattctg aataactaat accatacgca cgagaaatag catacgcagt
      121 ttcttttgct tgtactgtat gtatataata tttttttgca tcaatatttt caattacttg
      181 cgattttgca attaattgat tttgagaata tatagtatta ctaaaaaaac ttatacaaat
      241 aaaccatata ctaataatta tactattcct catacgcata ttataatttt gtacgctcgg
      301 tacaatttat tttcaaatag aatttatagt gcaaaaatat gcttttttta gtatttatat
      361 ctgttgatac atatttttac aattttataa tttttgaatt tctctatatc tttttcgtgc
      421 ctgaatacta aaataactac taggatattc ggttactaat gttttatagt gtaattgagc
      481 tttatcaggt tgttttgcta tataatcata atattcggca agcataaaat gggcattatc
      541 ggcatataca tcatatgaaa acctttgtgt aatttgaagt aaatagtctt ccatactttc
      601 aaatttttgt aatttttgag cagcaatagc ttttcgcata agcacatcat cttctaaact
      661 atgcccgggg aaattgtgta caatagaatc taaatataca tacgcctctg ttacattatt
      721 ttgatataat aacaaatcag cttttgaaaa atactgtaaa gcttttgtag aagtatccat
      781 tatagtattt tcagaaatca gaagcgacaa ttcaaatgca tcattggcaa ttaatttaga
      841 tgtactcgct tttaaaatat caagtaatgc ttgagcgtat tcaaattgag cattataata
      901 cgcaacttga gcttgtttaa atcgtgcttg atgccctata tcattatttt tgaaatctaa
      961 tgcaacttta gcgtacaaca aatttgcaga ccaaatatca tcagaaaaca agtacatatc
     1021 agcaagttcc attgaacaca accccttttc aacatatgaa attccgggta taagcaatga
     1081 cttttgcaat ctatcaattg cttcgctata tttatgcaaa taaaaacctt gaatatgagc
     1141 taaatttcga ataacatcgg ttgtattttg attttctcca atatcttgca aaactttaat
     1201 atactgactc tcaatatctt taattgcatc taccgaagga aattgcccca taaataaaga
     1261 tttataggta gtttccaaca tcatttttac agctgccata taatatggtt tatcttttcc
     1321 ttgatttacc acataagaga aacattcagt agcgattgcg aattcatcat ttgacagagc
     1381 tatttgacct acatcatata ctttttcacc aaaaccttgc tgcctgatat caagagaacg
     1441 tgcaattaca aacgcttttt taaacgtttt ttcttgcaaa taaaaccata gtaataattc
     1501 attaaattca acactttgag gattctcatt tacttttagt aatagttttt ccaataaaat
     1561 atcttgtaat tttgattgtg actgatcaaa taacaaattt tgcaactgat tttttataaa
     1621 ttccaattcc aatggattta cttcaagtaa atcaattaca gctgatgaaa gtttttcgta
     1681 ctgtaacaat ttagaatata ttgctatttg ttttttatat agtggttcaa aatcagcata
     1741 tttttttaca gccacttcta aaaaagtttg agccagttca tattgtttat tatcaataca
     1801 agcatctaca tattcaatac aatcaaatga attccgtaat gctctttttg tattt
//
LOCUS       DAOTOW010000485         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49009_length_1849_cov_5.239688, whole genome shotgun sequence.
ACCESSION   DAOTOW010000485 DAOTOW010000000
VERSION     DAOTOW010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49009_length_1849_cov_5.239688"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..592
                     /gene="ispF"
                     /locus_tag="P5243_08400"
     CDS             116..592
                     /gene="ispF"
                     /locus_tag="P5243_08400"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRS19510.1"
                     /translation="MKIRVGQGYDVHQLAEGLPFILGGIHIPHSHGMVAHSDGDVLLH
                     AICDALLGAAGIRDIGFHFPDTSQDFKNIDSRILLRKTYELISAKGWNIANIDATVCL
                     QKPKLLPHIPVMQETIAQDLQIDISQVSIKATTTEKLGFVGREEGIAVYAVALLLA"
     gene            631..>1849
                     /locus_tag="P5243_08405"
     CDS             631..>1849
                     /locus_tag="P5243_08405"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HRS19511.1"
                     /translation="MKIYTIHIYLILLLATYCTKKTYCQEYQIFTNEKRAEYILSFSK
                     NVTWPTPSFERFTIAIVSGDSLLYYRMQHLCDSVKTLHNKPISIHLYHAISSVKPCQV
                     LFIPFSKNINYEQLYAAIQQTQTLLLTENFPFNKSMINFTVNNGKRNYEINEQKLQEA
                     KLKVNPLLLKYAIKTKEDWEALYTKTEALLEKEKSTNQEKSEIIEKQIQHITNQEKTI
                     ANLTYSIEKQTLELQRLSRAVNNKQLELQQTLQQIQKRSDEIEIQELQLGSLKKNIKQ
                     QQEILRAQSNLIYSQENTLSLQKSKIQSQKIIIYLSISILILIAFFGYVLYRLYAKTK
                     RINALLQKKNEEIYEQNQHIARQNKEITDSIVYARRIQHAILPPQEIITQTLSEHFIL
                     FKPRDIVSGDYYWM"
BASE COUNT          748 a          308 c          244 g          549 t
ORIGIN      
        1 cctgtaaatg gcaccaattc acctattgct aatacatttc gtttttcact tacctttgat
       61 tttgacggtt tagcgcaaga aagtaaaggt agtatggata agtattaaaa aaaacatgaa
      121 aatacgtgta gggcaaggct atgatgtaca tcaattagca gaaggactac catttatttt
      181 aggtggcata cacatacctc attcacatgg tatggttgca cattccgatg gagatgttct
      241 gcttcatgct atttgcgatg ctttattagg agctgcaggc atacgtgata ttggtttcca
      301 ttttcccgat acatctcaag atttcaaaaa cattgacagt agaatactat tacgaaaaac
      361 ttacgaactc atatcggcta aaggttggaa tattgcaaat attgatgcaa cggtatgttt
      421 acaaaaacct aaattattgc ctcatatacc ggtcatgcaa gaaactatag ctcaagattt
      481 acaaatagat atttctcaag tatcaatcaa agcaactaca acagaaaaat taggatttgt
      541 aggaagagaa gaaggtattg ccgtatatgc agtagcttta cttttagcat aatctgtatc
      601 attttacaat ataaatagta tatttgaatt atgaaaatat atactataca tatatatctt
      661 atattattac ttgcaacata ttgcacaaaa aaaacctatt gtcaagaata tcaaatattt
      721 acaaatgaaa agcgagcaga atacattctc agtttttcaa aaaacgttac atggcctacc
      781 ccttcatttg aacgatttac aatagctata gtttcgggag attcattatt atattaccga
      841 atgcagcacc tctgtgattc tgtaaaaaca cttcacaaca aacctatctc tatacatctg
      901 tatcatgcta tttcttctgt aaaaccttgt caggttttat ttattccttt ttccaaaaat
      961 attaattatg aacaattata tgcagctatt cagcaaactc aaacattact attaacagaa
     1021 aattttccat ttaataaatc aatgataaat tttactgtta acaacggaaa acgaaattat
     1081 gaaataaatg aacaaaaatt acaagaagca aaactgaaag taaatcctct tttactaaaa
     1141 tatgcaataa aaacgaaaga agactgggaa gctctgtata caaaaacaga agccctactc
     1201 gaaaaagaaa aatctacaaa tcaagaaaaa tcagaaatta tagaaaaaca aatacaacat
     1261 attactaatc aagaaaaaac gattgctaac cttacctatt caattgaaaa acaaacttta
     1321 gaattacaac gactttcacg tgctgtaaac aataaacaat tagaattaca acaaacacta
     1381 caacaaatac aaaaacgaag tgatgaaata gaaattcaag aattacaact tggttcatta
     1441 aaaaaaaata taaaacaaca acaagaaata cttcgagctc agtctaattt aatttatagc
     1501 caagaaaata ctttatcatt acaaaaaagc aaaattcaat ctcaaaaaat tattatttat
     1561 ttaagtatta gcatacttat attgattgct ttttttggct atgtgttata tagattatat
     1621 gcaaaaacca aacgaataaa tgctctgctc cagaaaaaaa acgaagaaat ttacgaacaa
     1681 aaccaacaca tagctcgaca aaataaagaa attaccgata gcattgtgta tgctcgcagg
     1741 atacaacatg ctatattacc cccacaagaa ataattacac aaactctttc agagcatttc
     1801 atattattta aacctcgcga tatagtaagt ggcgattatt attggatga
//
LOCUS       DAOTOW010000486         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49018_length_1849_cov_3.452620, whole genome shotgun sequence.
ACCESSION   DAOTOW010000486 DAOTOW010000000
VERSION     DAOTOW010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49018_length_1849_cov_3.452620"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="P5243_08410"
     CDS             <1..1186
                     /locus_tag="P5243_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS19512.1"
                     /translation="SVKKTGVEPVFRQRQNQNKISINQQFTNKFSSKSNIKLGYSYDM
                     YAINFADSIYLAQFDTYRKGVDVKGNFSLLQAYIQNQCKPFESFTCNIGVHSQIFSLN
                     NQVVVEPRLGLEYAIAKNHVISFGYGIHNKTQPHEVYFQQFYDSITQTYYKPNTNLKF
                     TQNTHYILGYNTRMFDVWKCKLELYYQYIQHAPVSNAYPSYSMLNAGADFTVPRIENL
                     VNKGTGSNKGVEITIERVLEHGFYVLVTTSLFDSKYTDYNGKERNTAFNGNYIINALS
                     GYEITIKNNKMVTFDLRTVYAGGKRYIPIDIEASKQANDMVYNWNSIYEKKYKPYFRT
                     DLRIGYKVNGKKITQEWGLDLQNITNYKSIFMEAYDAQKQEVYYLYQQGFMPMMLYRI
                     NF"
     gene            1195..>1849
                     /locus_tag="P5243_08415"
     CDS             1195..>1849
                     /locus_tag="P5243_08415"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS19513.1"
                     /translation="MDEFNPVKIYNRKILLLLIPIIGAAISLLYFINPQNVQDHKRIV
                     FVLHSVIITSGIWLGCNTIVIFLWKQFPWEFYPIKHVIIEFILILVYTNLFSYGMYIL
                     LLHIGFIADKAVIIDLYTDVLVTNLITFLITAIHEAVFFYRQWKYNFSKSIQLEKEHI
                     QAKYDLLKSQIQPHFLFNSLNALYSLVFSNNQARNYVQNLSEFIRYSISHNDTKLVLF
                     "
BASE COUNT          675 a          233 c          292 g          649 t
ORIGIN      
        1 ttcagttaag aaaacaggag tagaacccgt gtttcgacaa agacaaaatc aaaataaaat
       61 atctataaat cagcaattca caaataaatt ttcgtcaaag tcgaatataa aactcgggta
      121 tagttatgat atgtatgcaa ttaattttgc cgatagtata tatttggctc aatttgatac
      181 ttatagaaaa ggtgtagatg taaagggtaa ttttagtttg ttgcaagcat atatacaaaa
      241 tcaatgtaaa ccgtttgaat catttacttg taatattggt gttcattcac agatattttc
      301 attgaataat caagttgtag tagaaccacg tttaggattg gaatatgcca ttgctaaaaa
      361 tcatgttatt tcttttgggt atggcattca caataaaaca caaccacacg aggtgtattt
      421 tcagcaattc tatgattcta ttactcaaac gtattataaa ccaaatacca atttaaagtt
      481 tacacaaaat acacattata ttcttggtta taatactcga atgtttgatg tgtggaaatg
      541 taaattagaa ttgtattatc aatatattca acatgccccg gtaagtaatg catatccctc
      601 atattcaatg ctcaatgccg gcgccgattt tactgtgcct cgtatagaaa accttgtaaa
      661 taaaggaacg ggttctaata aaggtgttga aattacaatt gaaagagttc ttgaacatgg
      721 attttatgta ttggttacaa catcattatt tgattctaaa tatacagatt ataatggaaa
      781 agagcgtaat acagctttta atggaaatta tattataaat gcactttcag ggtatgagat
      841 tacaattaag aataataaaa tggttacatt tgatttgcga actgtgtatg ccggaggtaa
      901 acgatatatt cctattgata tagaagcatc caaacaagca aatgatatgg tgtataattg
      961 gaattcaatc tatgaaaaaa aatataaacc atattttaga acagatctta gaattgggta
     1021 taaagtaaac gggaaaaaga taacacagga atgggggctt gatttacaaa atattacaaa
     1081 ttataagagt atatttatgg aagcatacga tgctcaaaaa caagaggtgt attatttata
     1141 ccaacaagga tttatgccta tgatgttgta tcgaattaat ttttaactta ctacatggat
     1201 gaatttaatc cggtaaaaat ttataatcga aagatattgt tgctcttgat tcctataatt
     1261 ggagcagcaa tatcgttatt gtattttata aatccacaaa atgttcaaga tcataaacgt
     1321 atagtatttg ttcttcattc agtgattatt acaagtggta tatggttagg gtgcaatact
     1381 attgttattt tcttatggaa acaatttccg tgggaatttt accctataaa acatgtaatt
     1441 atagaattta ttcttattct agtatatacc aatttatttt catatggtat gtatatatta
     1501 ctattgcata taggttttat agcagacaaa gctgttataa tagatttata tactgatgtg
     1561 cttgttacta atctaattac ttttttgatt acagcaattc atgaagctgt atttttttat
     1621 cgtcaatgga aatataattt ttcaaaatct attcagttag aaaaagaaca tatacaggca
     1681 aaatatgatt tactgaaaag tcagattcag ccacattttt tgtttaatag tttgaatgct
     1741 ttatattcat tagtattttc aaataatcaa gcacgaaatt atgtgcaaaa tttatcagaa
     1801 tttataaggt atagcataag ccataacgat accaaattgg tgttgtttc
//
LOCUS       DAOTOW010000487         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49030_length_1849_cov_2.524526, whole genome shotgun sequence.
ACCESSION   DAOTOW010000487 DAOTOW010000000
VERSION     DAOTOW010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49030_length_1849_cov_2.524526"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="P5243_08420"
     CDS             complement(<1..744)
                     /locus_tag="P5243_08420"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRS19514.1"
                     /translation="MTSALDLDIQFLPGVGPKRADLLKTELNLFTIRDVLQFFPFRYI
                     DRSKFYPICELQPAMASVQVKGTITNLQTEGSGHKTRLKATLYDGTGMLDLVWFTNIK
                     WVKEMLKPNTEYVVFRRISEFKSHISMSHPEIEEFKKFSAKNIQGFVGVYPSTEKIRN
                     ANIHGKTFRAMVEASFEKIGNEIISETLPAYILKEFGLPNLRETYKILHMPSNLADVS
                     KAQFRVKFEELFWLQLGMAFKKQVHTQYVK"
     gene            complement(744..1742)
                     /locus_tag="P5243_08425"
     CDS             complement(744..1742)
                     /locus_tag="P5243_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19515.1"
                     /translation="MKHLKKHIITIIILYISNIFAYSQTTISHTSYECIGDNREYFSP
                     YGFPQTILGSRISQDFIIQTDSLHSITLGGKYLLIHGANQFANTPVITAMYTYSNNYV
                     TFKTGSFPMQRNMFPLAMYTDSLYYFRPNMQGALVSYSNKMGFWNIEETGIFDWLLQE
                     NYGANESFLAGISGTLWYKNVFNKHYYYYRHHATDTPTGNKAVEDNGSVLLTLGYATH
                     TPITLSYEIGKLITWQELRGSMEKYSGGLFTQATISYNRLKADATYYFGDPTYLPLGD
                     PLYRSGNYARINTSLEFLKSKHVQGIFSFCTHIIAHEINFSQQLSILVNIDTNIGK"
BASE COUNT          589 a          279 c          309 g          672 t
ORIGIN      
        1 ttttacatat tgcgtatgaa cttgtttttt gaatgccatt cctaattgta accaaaacaa
       61 ttcttcgaat tttacacgga attgagcttt ggaaacatct gctaaattag aaggcatatg
      121 cagaatttta tatgtttctc gtaggtttgg taatccaaat tctttaagaa tataggcagg
      181 taatgtttct gaaattattt cgtttccaat tttttcgaat gacgcttcaa ccatagcgcg
      241 aaacgttttt ccatgtatgt tggcatttct tattttttcg gtactgggat atactcccac
      301 aaatccttga atattttttg cagaaaattt tttaaattct tcaatttcgg ggtgtgacat
      361 acttatatgc gatttgaatt ctgaaattct tcgaaatacc acatattctg tattaggttt
      421 aagcatttct tttacccatt ttatatttgt aaaccaaact aaatctaaca tgccggtacc
      481 atcatacaat gttgctttga gtcgtgtttt atgaccactg ccttctgttt gaagatttgt
      541 aatagtacct tttacttgaa ctgatgccat tgccggttga agttcgcata tagggtaaaa
      601 tttgctgcgg tctatatatc ggaaaggaaa aaattgtaaa acatcacgaa ttgtaaataa
      661 gttgagctct gttttaagta aatcggcacg tttaggccct acaccgggca aaaattgtat
      721 gtctaaatca agagcacttg tcattatttt ccaatatttg tatcaatatt tacaagaatt
      781 gaaagttgtt gcgaaaaatt tatttcatga gcaattatat gtgtacaaaa actaaatata
      841 ccttgaacat gttttgattt caaaaattct agtgaagtat ttattctggc ataattcccg
      901 cttcggtaca aaggatcgcc aagaggcagg taggtagggt caccaaaata atatgttgca
      961 tcagctttta aacggttgta tgaaatagtt gcttgtgtaa atagtcctcc cgaatatttc
     1021 tccatactac ctctcaattc ttgccatgtt ataagttttc ctatttcata tgatagtgta
     1081 atgggggtat gagttgcata accaagcgta agcaatacac taccgttgtc ttctactgct
     1141 ttgttcccgg taggagtatc tgtagcatgg tgtctgtaat aataatagtg tttattaaat
     1201 acatttttat accataaagt accacttata ccggctaaaa atgattcatt tgctccataa
     1261 ttttcttgta ataaccaatc aaatataccg gtttcttcta tgttccaaaa tcccattttg
     1321 tttgaataag acactaaagc tccttgcata tttggcctaa agtaatataa agaatcagta
     1381 tacattgcta acggaaacat attgcgttgc atggggaatg aacctgtttt gaaagttaca
     1441 taattattag aatatgtata cattgctgta attacaggag tatttgcaaa ttgatttgct
     1501 ccatgtataa gtaaatattt acctcctaag gttatactat gtaaactgtc agtttgtata
     1561 ataaaatctt gagaaattcg agaaccaagg atagtttgcg gaaaaccata tggtgaaaag
     1621 tattcgcggt tgtcaccaat acattcatat gaagtgtgtg aaatagtagt ttgactgtat
     1681 gcaaaaatat tagaaatata cagtataatt attgtaatta tgtgtttttt aagatgtttc
     1741 atgtaagaaa tattttaagg tataaaaata atctattttc atgaaacaaa cgaatttgac
     1801 atatatttgt aaaaaacttt tttgtatgaa acgattgttg tttatagtg
//
LOCUS       DAOTOW010000488         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49103_length_1847_cov_2.559710, whole genome shotgun sequence.
ACCESSION   DAOTOW010000488 DAOTOW010000000
VERSION     DAOTOW010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49103_length_1847_cov_2.559710"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="P5243_08430"
     CDS             <1..815
                     /locus_tag="P5243_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS19516.1"
                     /translation="MTKKSNIAMIAAIIIYLFVFMYGVMVLRGVIEEKTGRVVEILVS
                     SVKPFQLMAGKIIGIGTVGLIQFIVWIVLTFSIVYGAQVVLFPETYTPTPLPELQDSL
                     GNNNHSLLQLQSDTNVDYAINLFQAIDGVNWTIMLSSFVFFFIFGYLLYASLFAAIGA
                     FIDNDSETQQFVIPVTIPLMLTLFLLGNIVANPSGDIALWLSFIPFTSPIAMMARLPF
                     GVPYWHVLLSACILILTCLISIILSAKLYRTGILLYGKKIRLQTIISILMKN"
     gene            817..1365
                     /locus_tag="P5243_08435"
     CDS             817..1365
                     /locus_tag="P5243_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS19517.1"
                     /translation="MQKILVYILVYFTTALYSQTFTGGICGGITTSQINNDDVGGFYK
                     FGPTGGFFIQKKLQEFTALQYEIRYTAKGSSDGAGGLQINLGYIETPLLYKYSKHIPF
                     TFYGGLSPAIKLFETTSHSMVLRETNDFSRFELPFAVGISYNLTSTMYADVRYTQSTV
                     SAGGVHYWYLNQCLYFAVYKNL"
     gene            1370..>1847
                     /locus_tag="P5243_08440"
     CDS             1370..>1847
                     /locus_tag="P5243_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRS19518.1"
                     /translation="MKLAIGITGASGAIYAQLLMKACAEYKNQLDNVAIVFSPNAKDV
                     WHHELKISINTYGFTQYEYTDFNAPFASGSAGYDALVICPCSMGTMGKIAHGIADNLI
                     TRSADVMLKERKKLILAIRETPYNLIHIKNMELITLAGGIICPASPNMYSNPATIED"
BASE COUNT          595 a          312 c          320 g          620 t
ORIGIN      
        1 ccatgacaaa aaaatcaaac atagctatga ttgcggcaat aattatttat ttgtttgtat
       61 ttatgtacgg tgttatggtt ttacgaggtg tgatagaaga aaaaaccgga cgtgtagtag
      121 aaattctcgt atcatcggtt aaaccctttc agcttatggc cggtaaaatt attggtattg
      181 gaacggtagg tttaatacaa tttattgtat ggattgtact tacattttct attgtatatg
      241 gtgctcaagt agtgttgttc ccggaaacgt acactcccac cccattaccc gaacttcaag
      301 attctcttgg aaataataac catagtttac ttcaattgca gtccgacaca aatgttgatt
      361 atgccataaa tctgtttcaa gccatagatg gcgtaaattg gactataatg ctaagttcgt
      421 ttgtgttttt ctttattttt gggtatctat tatacgcatc attatttgca gctataggag
      481 cattcattga taatgattca gaaacacaac aatttgtaat tccggtaact attccactta
      541 tgctaacttt attcttgtta ggcaacattg tagcaaatcc ttcgggcgat atagcattgt
      601 ggttatcatt tattcctttt acttcgccca tagcaatgat ggctcgatta ccttttggtg
      661 tgccatattg gcatgtatta ctttctgcgt gtattttaat actcacatgc ctcattagta
      721 ttatactctc agcaaaatta tatagaaccg gaattttatt gtatggtaaa aaaattagat
      781 tacaaacaat aatctcaatt ctaatgaaaa actaatatgc aaaaaattct tgtatatata
      841 cttgtatatt tcacaacagc attatattct caaacattta ccggtggtat atgcggtggc
      901 attactacca gtcaaataaa caacgatgat gtaggtggtt tttataaatt tggacctaca
      961 ggtggatttt tcattcaaaa aaaactacaa gaatttaccg ctttacaata tgaaattcgt
     1021 tatacagcca aaggttcaag tgatggtgca ggtggcttac aaattaattt aggatatata
     1081 gaaactcctt tactatacaa atattcaaag catataccgt ttacgttcta tggaggttta
     1141 tcacctgcaa ttaaattatt tgaaacaacc tcgcattcaa tggtattgag agaaacaaat
     1201 gatttttcac gatttgaatt gccttttgct gtaggtatta gttataatct aacgtctact
     1261 atgtatgcag atgtgcgtta tacacaaagt acagtatcgg caggtggtgt tcattattgg
     1321 tatttaaatc aatgtttata ttttgcagta tataagaact tataattcta tgaaattagc
     1381 aataggtata acaggtgcaa gcggtgcaat atatgctcaa ttattgatga aggcttgtgc
     1441 cgaatacaaa aatcaactag ataatgttgc aatagttttt agccccaatg ccaaagatgt
     1501 atggcaccat gaattaaaaa tttcaataaa cacctatggt tttactcaat atgaatacac
     1561 cgatttcaac gctccatttg catcgggctc tgcaggatat gatgcattag ttatttgccc
     1621 ttgcagtatg ggaacaatgg gaaaaatagc tcacggtata gcagataatc ttataacccg
     1681 cagtgccgat gtaatgctca aagaacgcaa aaaacttata cttgccattc gtgaaacacc
     1741 gtataacctt atacatatta aaaatatgga acttattacg ttggccggag gcattatttg
     1801 cccggcatcg ccaaatatgt acagtaaccc cgctactata gaagatg
//
LOCUS       DAOTOW010000489         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49520_length_1837_cov_2.617845, whole genome shotgun sequence.
ACCESSION   DAOTOW010000489 DAOTOW010000000
VERSION     DAOTOW010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49520_length_1837_cov_2.617845"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1244)
                     /locus_tag="P5243_08445"
     CDS             complement(<1..>1244)
                     /locus_tag="P5243_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19519.1"
                     /translation="SATCFTENCYPLTFDKSQDLEICSGDTATLYIHNVSVPYYSVSW
                     NAQPFAQDTLKRYIFNKDTIIMVSVRNNSQLICNASKKYFKIKVHDYPLVSLETAEYT
                     DTVCSGSAVTFVAKPSGFDEYYFYDNWNLVQAGPSPVYVCNKYYNQHEILVRPKNNGC
                     LNMEGTSIKRDVIKPLDPPQVNCGKTTTGYVEFVWDSIPGANGYMVSVNDNTFIPANG
                     TFSHTVYTTPSTQVLIEVVATGNTPCGTSLSSSIWSGSLTMCETKPCEPINFTRPENF
                     AICAGQQANLEIQNLTEPTNYSVSWKGEPETTSLTYSYKPLQTDTASIIYWDKNQPGC
                     FPARKYTVIDVYDIPVTNLIAPQDTVCQGDNITIESTLQGMDTYDFYSKNNLLYSGNK
                     YWLPIYDIQKDTSIYLTVNFQG"
     assembly_gap    1245..1254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1255..1774)
                     /locus_tag="P5243_08450"
     CDS             complement(<1255..1774)
                     /locus_tag="P5243_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19520.1"
                     /translation="MTTKYIDVTVHKTPTVSLISSITADSICESTRVTFTAQSPEYQL
                     YEFYNNYDIVQSGVSPYYTTNELKDLQEIRVRATHYGCRSELSNPISYRVIKKLKQTQ
                     VVCGTTSVNSVNFLWDPVPGATGYMISVDSVGGVPNFKNVGNTTEYTISGLQLGDART
                     LTVKPYSNTACST"
BASE COUNT          555 a          286 c          330 g          656 t
ORIGIN      
        1 acccttgaaa attgacagta agatatatac ttgtatcctt ttgaatatca tatattggca
       61 accaatattt attacctgaa tataatagat tgtttttact gtaaaaatca taagtatcca
      121 ttccttgtaa agttgattct atggttatgt tgtcgccttg acaaacggta tcttgaggag
      181 ctataaggtt ggtaacagga atatcatata catctattac tgtatatttt cgtgcaggga
      241 agcaacctgg ttgattttta tcccaataaa taatagatgc agtatcggtt tggagtggtt
      301 tataagaata tgtaagtgag gtggtttctg gttcaccttt ccatgaaaca ctataatttg
      361 ttggttctgt tagattttgt atttctaaat tagcttgttg tcctgcacaa atagcaaaat
      421 tttccggtct tgtaaaattg attggttcac atggttttgt ttcgcacata gtaagtgaac
      481 cagaccaaat agaagatgat aaacttgtgc cacatggggt atttcctgtt gctaccactt
      541 ctattaaaac ttgagtagaa ggagttgtat atacagtatg tgaaaaagta ccatttgcag
      601 gaataaatgt gttatcattt acacttacca tataaccatt agctccggga atagaatccc
      661 aaacaaattc tacatatccg gtagttgttt ttccacaatt tacttgaggt ggatcaagcg
      721 gttttattac gtctcgttta atagaagttc cttccatatt caaacagcca ttatttttgg
      781 gacgcaccaa tatttcatgt tggttgtaat atttattgca cacatatacc ggcgaaggtc
      841 ctgcttgcac taaattccaa ttgtcataga aataatattc gtcaaaacca gatggctttg
      901 ctacaaacgt aacagctgaa ccggaacaaa cagtgtcagt gtattctgca gtttcaagac
      961 ttactaacgg ataatcatga acttttattt tgaaatattt ttttgaagca ttacatatta
     1021 attgactatt gtttctaaca cttaccatga ttatagtgtc tttgttaaaa atatatcttt
     1081 ttagtgtatc ttgagcaaat ggttgagcat tccatgatac agaatagtat ggtacagata
     1141 cattgtgaat ataaagtgtt gctgtgtcac ccgaacaaat ttctaaatct tgagatttat
     1201 caaatgtaag aggatagcaa ttttcggtaa aacaagtagc cgaannnnnn nnnncagtag
     1261 aacatgctgt attcgagtaa ggttttacgg ttaatgttcg tgcatcgcca agttgtaaac
     1321 cacttatagt atattctgta gtatttccta catttttaaa atttggaaca ccgccaacac
     1381 tgtctacact aatcatatat cctgtagcac cgggaacagg gtcccataag aagttaacac
     1441 tattaactga tgtagtacca catactactt gcgtttgttt tagttttttt attactctat
     1501 aactaattgg attcgataat tcacttctac atccgtagtg tgttgctcta actctgattt
     1561 cttgtaaatc ttttaattca tttgttgtat aataggggct tactccggat tgtacaatgt
     1621 cataattgtt gtaaaattca tatagttgat attcgggtga ttgtgcggta aacgtaacac
     1681 gtgttgattc gcaaatggaa tcagcggtaa tagaagaaat aagtgaaaca gttggtgttt
     1741 tatgaactgt aacatctata tatttggttg tcataggaca gtttggttca tcggtgtttt
     1801 ttacacttac atagtattga gtactttgtg ttggttt
//
LOCUS       DAOTOW010000490         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49554_length_1836_cov_2.653004, whole genome shotgun sequence.
ACCESSION   DAOTOW010000490 DAOTOW010000000
VERSION     DAOTOW010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49554_length_1836_cov_2.653004"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..1330
                     /gene="serC"
                     /locus_tag="P5243_08455"
     CDS             254..1330
                     /gene="serC"
                     /locus_tag="P5243_08455"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRS19521.1"
                     /translation="MKKHNFYAGPSRLSDYTLKATAEAIIDFAGTGLSIMGISHRTAE
                     FEDVMLSATEIVKELLDVPQGYSVLFLQGGASLQFCMAPFNLLENNATYINTGVWATG
                     ALKEAKYFGEVIELASSADRNFCYIPKGWEKNVSKDTSYVHITSNNTIYGTQYKSDPD
                     VPVYLVADMSSDLFSRPVDVSKYGLIYAGAQKNIGPSGATLVIIKDDILGKVSRPIPK
                     ILNYKTHIDGKSMYNTPPTVAVFACLKTLERYKQLGGVQAMKQRADAKAAMLYDEIDR
                     NKVFRGTADVEDRSNMNICFIMAEEYKELEGKFNEIVAARNILGIKGHRSVGGFRASV
                     YNAQREESVKALITAMKDFEKRYI"
     gene            1350..>1836
                     /locus_tag="P5243_08460"
     CDS             1350..>1836
                     /locus_tag="P5243_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS19522.1"
                     /translation="MTKVLVATEKPFAKEAVDGIVKIIEKAGFSYVLLESYSQKQQLL
                     DAVADVDAIIIRSDIVDKEVIEAAKQLKIVVRAGAGYDNIDLDACTAKGIVAMNTPGQ
                     NANAVAELVFGMMVFFARVGFNGKSGTELRGKTIGIHAFGNIGKLVAKIAKGFGMKVY
                     AY"
BASE COUNT          682 a          218 c          350 g          586 t
ORIGIN      
        1 aaaggaagac taacatcttc cttctcttaa ttttttatcg gacaacagtg atttagaaaa
       61 taaaaattaa aaataggtat tataaagaac gtaaaaataa tttttacagt agtataaaat
      121 aactgtttaa taaatagtta ctacgtttta tgcaattttc ttaaaatatt tttaaaatct
      181 tagggggtaa attaaatatt attttgcaaa tttgtgcttc atttttttca gaacaattaa
      241 tatacatttt tacatgaaga aacataattt ttatgcaggg ccttctcgat taagtgatta
      301 tacattaaaa gctactgcag aagccataat tgattttgcc ggaacaggtt tgtctattat
      361 ggggatttcg catcgtactg ccgaatttga agatgtaatg ctgagtgcaa cagaaattgt
      421 aaaagaatta cttgatgtac cacaaggcta ttcggtttta tttttacaag gtggggcaag
      481 tttacaattt tgtatggctc catttaattt attagaaaat aatgcaacat atatcaatac
      541 cggtgtatgg gctaccggag cattaaaaga agcaaaatat tttggtgaag taattgaatt
      601 ggcatcatct gccgatagaa atttttgtta tataccaaaa gggtgggaaa aaaatgtttc
      661 taaagataca agttatgttc atataacatc aaataatact atttatggta cacaatataa
      721 atcagatcca gatgtgcctg tatatttagt agctgacatg tcatcagatt tatttagcag
      781 accggtagat gtttcaaaat atggattaat atatgcaggt gctcaaaaaa atataggacc
      841 atctggtgca acattagtta taattaaaga tgatatttta ggaaaagtat caagaccaat
      901 tccaaaaatt ttaaattata aaactcatat tgatggtaaa tcaatgtata atacacctcc
      961 aactgttgct gtatttgcgt gtttaaaaac attggaacga tataaacaat taggtggtgt
     1021 gcaagctatg aaacagcgtg ccgacgcaaa agctgcaatg ttatatgatg aaatagacag
     1081 aaataaagta tttagaggaa ctgcggatgt tgaagaccgt tcaaatatga atatttgctt
     1141 tataatggca gaagaatata aagaattaga aggtaaattt aatgaaattg ttgcagctcg
     1201 taatatttta ggtattaaag gtcatcgttc agttggtggt tttagagcat cggtttataa
     1261 tgctcaacgt gaagagagtg taaaagcttt aataacagca atgaaagatt tcgaaaaacg
     1321 atatatttaa ataaatatac tggagcaata tgacaaaagt acttgtagcg accgaaaaac
     1381 catttgcaaa agaagcggta gatggtatag tgaaaataat tgaaaaagcc ggtttttctt
     1441 atgttttatt agaatcgtat tcacaaaaac aacaattgct tgatgctgtt gcagatgttg
     1501 atgctattat aattagaagt gatatagtag acaaagaagt aatagaagct gctaaacaac
     1561 ttaaaatagt agtacgagca ggtgccggat atgataatat tgatttggat gcttgtacag
     1621 caaaaggtat agtagcaatg aatactcccg gtcaaaatgc aaatgctgta gctgaattgg
     1681 tttttggtat gatggtgttt tttgctcgtg taggattcaa tggaaaatca ggaaccgaat
     1741 tacgagggaa aacaatagga attcatgcat ttggtaatat aggtaaatta gtagctaaaa
     1801 tagcaaaagg atttggtatg aaagtatatg catatg
//
LOCUS       DAOTOW010000491         1834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49616_length_1834_cov_2.783024, whole genome shotgun sequence.
ACCESSION   DAOTOW010000491 DAOTOW010000000
VERSION     DAOTOW010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49616_length_1834_cov_2.783024"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1834
                     /locus_tag="P5243_08465"
     CDS             <1..>1834
                     /locus_tag="P5243_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRS19523.1"
                     /translation="VEEHEKQTPAPWMKTVLHFGGGNTAAEKRLFKTYLQSYESSLEG
                     EYFGAQVYTFLKESSDVFEKTEPEAIREHMNNGTSILNFFGHASGTGFDQNIDHPSLF
                     NNQGKYPLIIANSCYSGDIFTTSTGINETWTLIPNRGSIGFIANVDLGSPTYLNIFTL
                     PFINNTAKHNYGKSVGTSIQLTLQEINNKYGSVQEIKNSSLSFMLHGDPAIKVHSFQN
                     PDLELKPASIFTNPSFVTNDLEEFTLNVVVYNNGRTTKDSCYLRVVLTPQSGEPYVIN
                     HLIQGLFLRDTFKFVLPIALFPAGNYSINAKIDDTNRIEEYDEMNNETTVDFFISSRD
                     VLPVYPPNYAIIPQNNQLLTVSAVDPHNPPARIIIEIDTTASYSSPLKVSQTIQTNGR
                     ASIQWNPGITYIEHKVYFWRVTNADSIKWNEHSFVYEQGRNGWAQKHPHQFIENNLSF
                     MNFNEQQNSYSYFTVPRVVTANNTGSLGYNVYPTNYLIDGVQYGSSGAGVDAIHIAVM
                     DSISLIPWYSSQDNYGHYNYLTGVGARYFIFVTSNAVYRANMAKFIRDSIPKGNYILA
                     FSYKDTRFSLWEEELRQVFDDLGATLHRTAPDSYPYILFTKKGDV"
BASE COUNT          628 a          338 c          298 g          570 t
ORIGIN      
        1 agttgaagaa catgaaaaac aaactccggc accttggatg aaaacagttc ttcattttgg
       61 aggtggtaat accgcagctg aaaaacgatt atttaaaaca tatttacaat catacgaatc
      121 aagtttagaa ggagaatatt ttggtgccca ggtttataca tttttaaaag aaagcagcga
      181 tgtgtttgaa aaaacagaac ctgaagctat tcgtgaacat atgaataacg gcacatctat
      241 acttaacttt tttggacatg cgtcgggtac tggtttcgac caaaatattg accatccttc
      301 attattcaac aatcaaggaa aatatccgct tataattgca aattcatgtt attcgggtga
      361 catatttact acttctaccg gcataaacga aacatggaca ctcattccaa accgtggaag
      421 cataggattt attgccaatg tagatttagg aagccctacg tatttgaata tttttactct
      481 tccttttata aataataccg ccaaacataa ttacggcaaa tctgtaggaa caagtattca
      541 acttacatta caagaaataa ataacaaata tggttctgtg caagaaatta aaaattcttc
      601 acttagcttc atgttacacg gagaccccgc tataaaagtt cactcatttc aaaatcctga
      661 tttagaatta aaacctgcat caatatttac caatccaagt tttgtaacca acgatttaga
      721 agaatttacc ctcaacgtag tagtttacaa caatggtcgc acaaccaaag acagttgcta
      781 tttacgagta gtacttacac cacaatcggg tgaaccatat gtaattaacc atctaattca
      841 aggtctattt ttgcgtgata cttttaaatt tgtattacca attgctcttt tccctgccgg
      901 aaactattca ataaatgcaa aaattgatga tactaataga atagaagaat atgatgaaat
      961 gaataatgaa acaactgtag acttttttat tagttcacgt gatgtattgc cggtatatcc
     1021 tccaaactat gctataattc cacaaaataa tcaattactt acagtttcag ctgttgaccc
     1081 acataatcca ccggcaagaa ttattattga aatagatact acagcctcat attccagtcc
     1141 actcaaagtg tcgcaaacta tacaaaccaa tggaagagct agtattcagt ggaatccggg
     1201 aattacatat attgaacaca aagtgtactt ttggcgggtt acaaatgctg attctataaa
     1261 atggaatgag cattcatttg tatatgaaca agggcgtaat ggttgggctc aaaaacaccc
     1321 gcatcaattc attgagaata acttatcgtt catgaacttt aatgaacagc aaaattcata
     1381 ttcatacttt accgtgcctc gagtagttac tgcaaacaat accggaagct taggatacaa
     1441 tgtataccct acaaattatt taattgatgg agttcaatat ggttcaagtg gtgccggcgt
     1501 agatgctata cacatagctg taatggattc aatttctctt attccatggt attcaagcca
     1561 agataactac ggacattaca attatttaac cggagttggt gctcgttact ttatttttgt
     1621 aacttcaaat gccgtatata gagcaaatat ggctaaattt attcgcgatt ctatccctaa
     1681 agggaattat attcttgctt ttagttataa agatacccga ttttctcttt gggaagaaga
     1741 attgcgtcag gtttttgatg accttggtgc tacacttcac cgtactgctc ccgattcata
     1801 tccctatata ttatttacaa aaaaaggcga tgta
//
LOCUS       DAOTOW010000492         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49648_length_1833_cov_3.113611, whole genome shotgun sequence.
ACCESSION   DAOTOW010000492 DAOTOW010000000
VERSION     DAOTOW010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49648_length_1833_cov_3.113611"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5243_08470"
     CDS             <1..441
                     /locus_tag="P5243_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS19524.1"
                     /translation="YLHDIARSLAPKFKGRNVTVDISCSQEIIFTSYPGAFAQIFTNF
                     IVNSLMHAYSETDTGTIRISVNENDSDFTFVYSDDGKGIPKENQPKVFEAFFTTNTQE
                     GTGLGMNITYNLVTQKLGGDIQLWSEEGKGVIFTIIIPKSNILA"
     gene            448..>1833
                     /locus_tag="P5243_08475"
     CDS             448..>1833
                     /locus_tag="P5243_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRS19525.1"
                     /translation="MKILTKELIHQADSYTIVNEPIASIDLMERASKAFVDELLEIVP
                     LARIFCVVCGSGNNGGDGFAIARILAKQHYSVKVFHIQSHKPLSRDCTCNLERLQNIV
                     SPTLDISIVSLTDISELEIPAQAIIIDAIFGSGLHTSIQGLSKECIIKLNALPNIRVA
                     VDIPSGLYCDRPQQVGDTIFKAHYTITFQYPKLSFLFLENEQFVGDWSVVPIGLSKEF
                     EEMVSTPYFFTMHCDVTLQSYSRFAHKGNKGHALIVAGSTGKTGAAVLAARACISSGC
                     GLVTAHVPQNSNTIMQIAIPEVMTSIDEGLDIIQSIPNFNSISAIALGPGIGTHSKTQ
                     QALIQYISHASIPMVLDADALNIVSLHKEILSKLPQDTILTPHPGEFDRLTRKHESSF
                     ERLQTQIAFSKQTKCIVVLKGKYTSITLPNGDVWFNSTGNHGMATAGSGDTLTGIIVS
                     LLAQGYTPNQAS"
BASE COUNT          613 a          291 c          326 g          603 t
ORIGIN      
        1 tatttgcacg atatagcacg tagtcttgca cctaaattta aagggagaaa tgttactgtt
       61 gatatttcat gtagtcaaga aattatattt acaagctatc cgggggcgtt tgctcaaatt
      121 tttacaaatt ttattgtaaa ttcattaatg catgcgtatt cagaaacaga tacaggaact
      181 attcgaattt ctgtaaatga aaatgattct gattttacat ttgtatactc cgacgatggt
      241 aaaggtattc ccaaagaaaa tcaacctaag gtatttgagg ctttctttac aaccaatact
      301 caagaaggaa caggattggg tatgaatatt acatacaatt tggtaacaca aaaattaggt
      361 ggtgatattc aattgtggag cgaagagggg aaaggagtaa tttttactat cataatacca
      421 aaatctaata ttcttgcata atcgtgtatg aaaattctaa ccaaagaatt aattcaccaa
      481 gccgattctt ataccattgt caatgaacca atagcttcta ttgatttaat ggaacgtgca
      541 tcaaaagcct ttgttgatga acttttagag attgttccgt tagctcgtat attttgtgtt
      601 gtttgtggca gcggtaataa tggcggagat gggtttgcta ttgctcgtat tttagctaaa
      661 caacactatt ctgttaaagt ttttcatata caatcacata aacctctttc tcgtgattgt
      721 acttgtaatt tggagcggtt acagaatata gtttcgccaa cgttagatat ttctattgtt
      781 tcacttacag atatttctga acttgaaata cctgctcaag caataataat agatgctata
      841 tttggaagtg gcttacatac ttcaatacaa ggattatcaa aagaatgcat tataaaactt
      901 aatgctttac caaatataag agtagcagta gatattcctt caggattgta ttgtgatagg
      961 cctcaacaag ttggagatac aatttttaag gctcattata caattacgtt tcaatatcct
     1021 aaactgtcat ttttatttct agaaaatgaa cagtttgttg gtgattggtc agttgttcct
     1081 attggtttgt caaaagaatt tgaagaaatg gtttctacac cttacttttt tacaatgcat
     1141 tgtgatgtta cgttacaatc atattcacgt tttgcacata aaggaaataa aggacatgct
     1201 cttattgtag caggaagtac aggtaaaacc ggagctgcag tgttagcagc tcgcgcatgt
     1261 ataagttccg gttgtggatt ggtaactgct catgtgcctc aaaattcaaa tactataatg
     1321 cagatagcta tacccgaagt tatgacaagt atagatgaag gactggatat aattcaaagt
     1381 ataccaaatt ttaattcaat ttctgcaata gctttaggac cgggtatagg aacacattct
     1441 aagacacagc aagctcttat tcaatatatt tcacatgctt caataccaat ggtgcttgac
     1501 gccgatgctt taaatatagt atctttacat aaagaaattc tttctaaatt accacaagac
     1561 acaatactta ctccgcatcc cggtgaattt gacagattaa cacgcaagca tgaatcttct
     1621 tttgaaagat tacaaacaca aattgcattt tctaaacaaa caaagtgtat agttgtttta
     1681 aaagggaaat atacaagcat aacgttgcca aatggtgatg tttggtttaa tagtaccgga
     1741 aatcatggaa tggcaacagc aggaagtggt gatactctta ccggaattat agtttcatta
     1801 ttagcacaag ggtatacacc taatcaagct tca
//
LOCUS       DAOTOW010000493         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49918_length_1826_cov_3.592885, whole genome shotgun sequence.
ACCESSION   DAOTOW010000493 DAOTOW010000000
VERSION     DAOTOW010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49918_length_1826_cov_3.592885"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(521..1471)
                     /locus_tag="P5243_08480"
     CDS             complement(521..1471)
                     /locus_tag="P5243_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRS19526.1"
                     /translation="MYTADPSAHVFNGKLYIYPSHDIDAGIPFDDEGSHFGMKDYHVI
                     SMDSIPGKTIDHGIILHIQDVAWAQKQLWDSDVNYKNGTYYLYFSAKSYDGIFRIGVA
                     TSKSPTGPFIAEPEPIKGSYSIDPCVFQDNDDSFYMYFGGIWGGQLQKYRNNIYNSLH
                     ELPRSHEPALGPRIAKLRDNMLEFAETPKEIEILDEQGNRLKEGDNSRRFFEASWMHK
                     YNGIYYFSYSTGDTHYICYATGISPYGPFTYRGRILNPVVGWTTHHSICEFQGKWYLF
                     YHDSSLSKGVTHLRSMKVTPIHYNADGSIQTIDPYNDLKI"
     gene            complement(1573..>1826)
                     /locus_tag="P5243_08485"
     CDS             complement(1573..>1826)
                     /locus_tag="P5243_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS19527.1"
                     /translation="GLKSGLSIGGALVSLILGQYGYIHAEAGETILQPQTVAHGVKMM
                     VSIYAALPFLICCALLFFYEINKTTEIQIESDLKKRRNN"
BASE COUNT          622 a          276 c          286 g          642 t
ORIGIN      
        1 catatgtgtt ttgtaaaaaa aatgtgcaca aaaaaataat tacgagtata gtattgaatt
       61 gtttcatatg tatattttta taatttctct caatattttt tagacaatca atatgtctta
      121 aaccctatat atttgcttgt gtagatgata aaattaaaat atgaaaaaca gaatcaaaaa
      181 cacaaacttt tagaacctat aaaattatat aaaatcaaaa tatatactat aggtattttt
      241 gggtattttc tgaaaaaaag aggcaggaag aaatcctgcc tcgcatccta aataaatact
      301 aacctataag gtttattgaa gaagataaac aagatttctc ttgttgagca tagctgctct
      361 gtaatactgt ttttatactt caaaaatata tgtttatata gttaaaacct attttgttgt
      421 atttaaaatt acgttaaaat tggtgaatga caaaaaacaa agggtaaaaa gtaatcatat
      481 aaaggtaaaa atgtaaagca ttagttcatt atttgtaact ctatattttt aaatcattat
      541 atgggtcaat tgtttgaatg ctgccatcgg cattataatg aatgggtgta actttcatag
      601 atcgtaaatg agtaacacct tttgaaagac ttgagtcatg ataaaataaa taccattttc
      661 cttgaaattc gcaaattgaa tgatgagttg tccaaccaac taccggattt aatattctac
      721 ctctatatgt aaaaggtccg taaggcgaaa tgcctgttgc gtaacaaata taatgtgtat
      781 cgccggtaga atatgaaaaa taatatattc cattgtattt atgcatccac gaagcttcaa
      841 agaatcggcg tgaattatca ccttctttta atctgttgcc ttgttcatcg agtatttcta
      901 tttctttggg tgtttctgca aattccaaca tattatcacg taattttgca attcgtggtc
      961 ctaatgctgg ttcatgagaa cgaggtaatt catgcaaaga gttataaata ttattcctat
     1021 atttttgtaa ttgtccaccc cagatacctc caaaatacat ataaaaggag tcatcattat
     1081 cttgaaatac acaaggatct atggaataac ttccttttat tggttccggt tcagcaataa
     1141 atggtccggt agggcttttt gatgttgcta cgccaatacg aaaaattcca tcataggatt
     1201 tagctgaaaa atagagataa tatgttccat ttttgtaatt tacatcagag tcccataatt
     1261 gtttttgagc ccatgcaaca tcttgaatgt gtaatataat tccgtgatca attgttttac
     1321 cgggtattga atccattgat attacatgat aatctttcat gccaaaatgt gagccttcat
     1381 catcaaaagg aataccggca tcaatatcat gcgatggata aatgtatagt tttccattga
     1441 aaacatgtgc tgatggatct gcggtgtaca tgtgagttac taatggttgc gatattgctc
     1501 gtgtattcaa catgtcaaaa tctatgtggt ctatactgtc ttctggcata atactttgtt
     1561 tttaataata aattagttgt ttcttcgctt ttttaaatca gattcaattt gaatttcagt
     1621 tgttttgtta atttcataaa aaaacaataa tgcacaacaa atcaaaaagg gtaaagcagc
     1681 atatatgcta accatcattt taactccatg tgctacagtt tggggttgta atattgtttc
     1741 acctgcttcg gcatggatat atccgtattg tcccaatatc aatgaaacaa gtgctcctcc
     1801 aatagataag cctgatttaa gtccta
//
LOCUS       DAOTOW010000494         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49925_length_1826_cov_2.999435, whole genome shotgun sequence.
ACCESSION   DAOTOW010000494 DAOTOW010000000
VERSION     DAOTOW010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49925_length_1826_cov_2.999435"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..>725)
                     /locus_tag="P5243_08490"
     CDS             complement(185..>725)
                     /locus_tag="P5243_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19528.1"
                     /translation="RTTFDPNKHNLYLQEVDFISKKHLFTYIASIESKGEYNVVSNQS
                     MLGKYQASKSSLREFGYTDNQIDSIYKTVYEVTISGNKKRYFFDTELFPPSEQERFIR
                     WYTNRMERVLLKYHIDEYVGRTIDGVYITKAGLLNASMLGHTHVIRFLESNGAINYSP
                     KCGYSVKERMQQLENTELH"
     assembly_gap    726..735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1040..>1826)
                     /locus_tag="P5243_08495"
     CDS             complement(1040..>1826)
                     /locus_tag="P5243_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRS19529.1"
                     /translation="YSTMSETLDYIDTYILEKQLDEYIQLISQAKKQLLIPVIASINC
                     ISDYEWTKFAKRIEIAGADAIELNIFLPASDETETNFEQTITSIISQVSSTVTIPIAV
                     KISSSFTKISKTIQIISQSNVAGIVLFNRFYSPDIDLDTLEIINSNRYSSAQEYTLPL
                     RWIALNSSKVSCDLIASTGIHTGDAVIKQLLVGASAVQIVSALYLHGFDYIQTMLQQL
                     ETFMQSKGYQYIDQFKGKLAVTSHNAASFERIQFMKYFAEIGK"
BASE COUNT          613 a          248 c          282 g          673 t
ORIGIN      
        1 acgtttgaaa aaaacagcca aaatgcaatc gcatacagca ttagaattag aaaaacaaca
       61 ttatcaacga ttgttagaaa gagaacagat tgaacataaa aattctaaaa cttattagaa
      121 ttaattaata tgtttaaaat aattggagaa catgcagcta acataattcc tgcttcatag
      181 ttgattaatg aagttctgtg ttttctaatt gttgcatgcg ttcttttact gaataaccgc
      241 atttagggga ataattaata gctccgttag attctaaaaa acgaataaca tgtgtatgtc
      301 ctaacataga agcatttagt aaaccagctt tggtaatgta tacaccatca atagttctgc
      361 ctacatattc atctatatga tatttcaata atacacgttc cattctgttg gtataccaac
      421 gaataaatcg ttcttgttct gaaggcggaa ataattcagt atcaaaaaaa taccgttttt
      481 tattgccgga aatagttact tcatatacag ttttgtatat actatcaatt tgattgtcgg
      541 tataaccaaa ttctcgtaat gaacttttac ttgcttgata tttgccaagc atagactggt
      601 tgcttacaac attgtattca ccttttgatt caatactagc tatgtatgtg aataaatgtt
      661 tttttgaaat aaaatctact tcttgtaaat ataaattatg tttgttgggg tcaaacgttg
      721 tacgannnnn nnnnnacctg aatgtatttc aagcaaagtt tggagctgca ataagtatta
      781 aaagcccaaa caccttaaaa taattggttt ttttgttcat ttttaatgtt tttcttatga
      841 gacgtttctc ataaaatttt catgcaaatg tatatgtaat atatttaaaa aaacaagtgt
      901 tgagtgttaa attaggtgaa tttacgtaat aaaaatatta cttttactca ataaaatatt
      961 gttgatatac agactgttat agcttccggc atatcaatat tccactaggt ttttaatgat
     1021 tgtatttctt ttagattttt tattttccta tttcagcaaa gtatttcata aattgaattc
     1081 gttcaaatga agctgcattg tgtgaagtta cagctaattt tcctttgaat tgatcaatat
     1141 attggtatcc ttttgattgc ataaaggttt ctaactgttg aagcatagtt tgaatataat
     1201 caaacccgtg taaatacaga gcagaaacaa tttgaacagc actagcacct acaagtagtt
     1261 gttttataac tgcatctcct gtatgaattc ctgtagaagc tattaaatca catgaaactt
     1321 tactggagtt taaggcaatc caacgaagag gtagagtata ttcttgagct gaactgtatc
     1381 tgtttgaatt tataatttct aatgtgtcta aatctatatc ggggctatag aatctgttga
     1441 acaatacaat tcctgcaaca tttgattgtg aaattatttg tattgttttt gatattttag
     1501 taaaagaact tgaaatttta acagcaatag gtattgtaac agttgaagaa acttgtgaaa
     1561 taatagacgt tatagtttgt tcaaaattag tttctgtttc atctgatgca ggcaaaaata
     1621 tattgagttc aatggcgtcg gcacctgcta tttctattct ttttgcaaat ttagtccatt
     1681 catagtctga aatacaattg atactggcaa ttacaggaat aagcagttgt ttttttgctt
     1741 gtgaaattag ttgaatgtat tcatctaatt gtttttctaa aatatatgta tcaatatagt
     1801 ctagtgtttc agacattgta gagtaa
//
LOCUS       DAOTOW010000495         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49958_length_1825_cov_3.122034, whole genome shotgun sequence.
ACCESSION   DAOTOW010000495 DAOTOW010000000
VERSION     DAOTOW010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49958_length_1825_cov_3.122034"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="P5243_08500"
     CDS             <1..577
                     /locus_tag="P5243_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRS19530.1"
                     /translation="IIVNTDSNTVFLKHTGMKISFGAIGKGYAANKARDAMKLAGIHS
                     GIVIAGGDLITWGSPEDTKLWTIGIADPREPEKAVSWLDVGEMSVVTSGTYEKFTEIN
                     GIRYAHIINPKTGYPATGLISATIICPDAELGDALATTCMLLGEQKALELINKIKGVE
                     CILITQDFRIIASNNIQLQPYTVQSTYRIIK"
     gene            585..803
                     /locus_tag="P5243_08505"
     CDS             585..803
                     /locus_tag="P5243_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HRS19531.1"
                     /translation="MNTIVRYIIIGIAMIALHSCVSVEAYQKAYLNDEDMELDARKVE
                     YYEIGIENYREGSSGANGGKGGGGCGCN"
     gene            788..>1825
                     /locus_tag="P5243_08510"
     CDS             788..>1825
                     /locus_tag="P5243_08510"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HRS19532.1"
                     /translation="MRLQLIYTVIFMLFAFNSYAQQTVIQEDPEINFLFNYYEQEGNH
                     SAVTGGIGTEQLKNMAPMIIVNIPVDSARIVNVSIGEDYYSSASSNKIVKYISEASKK
                     YLSSASKSDARYHGNIAYSKRNLSNYRTNTYMLGFSKEFDVISSNIGFGYLKTSKNEN
                     SSIQFKNLLYYDVWKIILPGEFRDSSKTHFREYPMHLGNSSTDYARDTRISNTFSIVY
                     TQVINKKLQVALLSDFVYQYGLLYTPFNRVFFDDGQDNDTIKVWNSAVDVELLPNTRY
                     KVPLGIRVHYFPVDFMVVRMLYRYYFDDFGIRSHTLQVELPLKPSPYLTLFPIVRYYT
                     QTASRYFYEYGT"
BASE COUNT          618 a          309 c          363 g          535 t
ORIGIN      
        1 cattattgta aataccgata gcaataccgt atttttgaag cacacaggca tgaaaatatc
       61 gtttggtgcc ataggtaaag ggtatgctgc caataaagcc cgcgacgcca tgaaattggc
      121 aggaatacac agtggaattg taatagcagg aggcgattta attacctggg gtagcccaga
      181 agataccaaa ctatggacta taggcatagc cgacccacgt gaacctgaaa aagcagtgtc
      241 gtggctcgat gtgggcgaaa tgagtgtagt aacatcgggt acctacgaaa aatttactga
      301 gattaatgga attcgttatg cgcacataat aaatcctaaa acagggtatc ctgctacggg
      361 attgatatcg gcaaccatta tatgccccga tgccgaattg ggtgatgcac ttgctactac
      421 gtgtatgctg ctgggagaac aaaaagcttt agaacttata aacaaaataa aaggtgtaga
      481 atgtatacta ataacgcaag attttagaat tatagcgtct aataacatac aattgcaacc
      541 atatacggtg caatctactt atagaattat aaaataaaca agcaatgaac acaattgttc
      601 gatacattat aataggtata gcaatgattg ctctgcattc gtgtgtaagc gtagaagcat
      661 accaaaaagc atacttaaac gacgaagata tggaactcga cgcacgcaaa gtagagtatt
      721 atgaaattgg tattgaaaat tatcgcgagg gttcgtcggg tgccaatggc ggaaaaggag
      781 gcggaggatg cggctgcaat taatttatac ggtaattttt atgttgtttg catttaatag
      841 ttatgcacaa caaacggtaa tacaagaaga ccccgaaatt aattttttgt ttaattatta
      901 cgaacaagaa ggaaatcatt ctgccgtaac cggtggtata ggcaccgaac agttgaaaaa
      961 tatggctcct atgataattg taaatattcc tgtagatagt gcccgcattg taaatgtatc
     1021 aataggcgag gattactatt catcggcatc gagcaacaaa attgtaaaat atataagcga
     1081 agcaagtaaa aaatatttgt cgtcggcatc aaaatccgat gcacgatatc atggaaatat
     1141 agcatatagc aagcgaaatc tttcgaacta taggaccaat acttacatgt tgggattttc
     1201 gaaagaattt gatgttattt catcgaatat tggtttcggc tatcttaaaa cttccaaaaa
     1261 cgaaaattca tcaattcaat tcaaaaattt attgtattac gatgtatgga aaattatatt
     1321 gccgggagaa tttcgtgatt cgtcaaaaac acattttaga gaatatccta tgcatttagg
     1381 taattcgtct accgactatg ctcgcgatac gcgaatttct aatacatttt ctattgttta
     1441 cacgcaggta attaacaaaa aactacaagt agccttgttg tctgatttcg tatatcaata
     1501 tgggttgttg tatacaccat tcaatcgtgt gttttttgat gatggacaag acaatgatac
     1561 cataaaagta tggaattctg ctgtagatgt agaattatta ccaaacacac gttacaaagt
     1621 tcccttggga attcgggtac attattttcc tgttgatttt atggttgtaa gaatgctgta
     1681 tcgatattat tttgatgatt ttggcatacg ttcgcatacc ctacaagttg aacttccgct
     1741 aaaaccatca ccttatctta cgttattccc tatagtgcga tactatacac aaacagcgtc
     1801 acgctatttt tatgaatatg gcacg
//
LOCUS       DAOTOW010000496         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_49981_length_1824_cov_3.721311, whole genome shotgun sequence.
ACCESSION   DAOTOW010000496 DAOTOW010000000
VERSION     DAOTOW010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49981_length_1824_cov_3.721311"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="P5243_08515"
     CDS             complement(<1..475)
                     /locus_tag="P5243_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRS19533.1"
                     /translation="MIPSSDVTLPQSTMVLYDVLRVYNTIPLFTELHAQRFFDSFTLA
                     KLPQPFMRHVFETSIYSLISREKIIEGNIRCAYYVCDNPLFIVYQISHSYPTDSMYKH
                     GVEVATLNAMRENPNVKQELHVRTIANSVIAEKGVYDVLLIDADGNITEGSRTNIF"
     gene            complement(604..1230)
                     /locus_tag="P5243_08520"
     CDS             complement(604..1230)
                     /locus_tag="P5243_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS19534.1"
                     /translation="MERKIYFNTHFVILSDGKNTKFSGETYICNNKTEILHIITTYIN
                     NPTQDICIVYHDFESLCNEVTNQFKYIEAAGGIVENEHHAIVCIYRNNMWDLPKGKAE
                     KGETPEQTAIREVCEETGLHSVSIHSYICSTWHTYQHPKGMILKQTYWYAMNSHSEQK
                     LIPQIEEGITQIDWIPLSHISTVYSQTYQSIRDVLNSFYHTSNRNTQI"
     gene            1229..>1824
                     /gene="pyrE"
                     /locus_tag="P5243_08525"
     CDS             1229..>1824
                     /gene="pyrE"
                     /locus_tag="P5243_08525"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRS19535.1"
                     /translation="MTSTEQLIAEHLLQINAIKLQPANPFTWASGWKSPIYCDNRKTL
                     SYPAIRKTIAQAFAQKIQELYPNVQVIAGVATGAIAVGVLTAEELGLPFIYVRSSSKA
                     HGLTNVIEGYFEKGQSVVVIEDLISTGGSSLKAVESLREAGCTVLGMLAIFTYGFDTA
                     VQNFEKAHCSLHTLSNYNALIALAIEKKYITKDQVLSL"
BASE COUNT          602 a          270 c          317 g          635 t
ORIGIN      
        1 aaaagatatt tgtacgactt ccttctgtaa tgttcccatc agcatctatt aataaaacat
       61 catacactcc tttttctgca attacagaat ttgcaatggt gcgtacatgt aattcttgtt
      121 ttacatttgg gttctcgcgc atagcattga gtgtggctac ttcaacacca tgtttataca
      181 tggaatcggt agggtatgaa tgtgatattt gatatactat aaatagagga ttatcgcata
      241 cataatatgc acatcgaata ttgccttcaa taattttttc gcgagaaata agtgaatata
      301 ttgatgtttc aaacacatgg cgcataaatg gttgaggtaa ttttgccaat gtaaatgaat
      361 caaaaaatcg ttgcgcatgt aattcagtaa ataaaggtat tgtattataa acccgtaata
      421 cgtcatataa taccattgta gattgaggaa gagttacatc agaactgggt atcacttttc
      481 catttacaca aacaaatttt gaaatacaac cgggtatcat ttacaatagt aatttttaaa
      541 aaagtaaaat tataaaaaac aaactatttg caagatgttt cataagaatt tttaaacagt
      601 ttgttatatc tgtgtgtttc tattacttgt atggtaaaat gaatttaaaa catcgcgaat
      661 agattgatag gtttgtgaat atacagttga aatgtgtgat agaggaatcc aatctatttg
      721 tgtaatgcct tcttcaattt gtggtataag tttttgttca gaatgtgaat tcatggcata
      781 ccagtaggtt tgttttagaa tcattccttt agggtgttgg tatgtatgcc atgtagaaca
      841 aatatatgaa tgtattgata cagaatgtag gcctgtttct tcacatactt cacgtatagc
      901 agtttgttcg ggtgtttcac ctttttctgc ttttccctta ggcaaatccc acatattatt
      961 tctatagatg catacaatag catgatgttc attttctact attccgccag cggcttctat
     1021 atatttgaat tgattggtta cttcattgca aagagattca aaatcgtgat atactataca
     1081 tatatcttgt gttggattgt taatatacgt ggtaattata tgtaaaattt cagttttgtt
     1141 attacatatg tacgtttcac ccgaaaattt tgtattttta ccgtccgata aaataacaaa
     1201 atgtgtattg aaataaattt ttctttccat gacttcaaca gaacaattaa ttgcagaaca
     1261 tttattgcaa attaacgcaa ttaaattaca accagcaaat ccttttacat gggcaagcgg
     1321 ttggaaatcg ccaatttatt gcgacaatcg taaaacttta tcatatcccg ctatacgaaa
     1381 aacaatagca caggcatttg cacaaaaaat tcaagaatta tatccaaatg ttcaagttat
     1441 agcaggagta gcaacaggag caatagctgt aggtgttctt accgccgaag aattaggatt
     1501 accatttatt tacgtgcgtt cgtcttctaa agctcatgga ctgacaaatg taattgaagg
     1561 atattttgaa aaagggcaat cagttgtagt tatagaagat ttaatttcaa cgggaggcag
     1621 tagtcttaaa gctgtagaat ctttgagaga agccgggtgt acagtattgg gtatgctggc
     1681 aatttttacc tatggttttg atactgcggt acaaaatttt gaaaaggctc attgttcatt
     1741 acatacgctt agtaattata atgcgttaat agcattagct atcgaaaaaa aatatattac
     1801 taaagatcaa gttcttagtt tgca
//
LOCUS       DAOTOW010000497         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50057_length_1822_cov_5.869270, whole genome shotgun sequence.
ACCESSION   DAOTOW010000497 DAOTOW010000000
VERSION     DAOTOW010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50057_length_1822_cov_5.869270"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1703
                     /locus_tag="P5243_08530"
     CDS             372..1703
                     /locus_tag="P5243_08530"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRS19536.1"
                     /translation="MDFTISQLGKCTVRTPLQLSKTKNDGIYDYIEDTERILFSNSLH
                     IVAHECKNNVMPASFEKPGPKEFLYFEPAKTKVAIVTCGGLCPGLNNVIRGLVNHLWE
                     IYKVRKILGVQYGYAGLNPENELPFLELNPEIVDDIHKNGGSMLGSSRGNQPVDIIVD
                     TLERHNINILFCIGGDGTLRGAHAIHQEITRRGLKISIAGIPKTIDNDINFIDKSFGF
                     ETAFTAAQEIILNAHNEAKGTQNGVALIKLMGRDSGFIAANAALAVPDVNFVLIPEME
                     FDLDDTETQNGFLNHLERRLIRKKHAVIVVAEGAGQHLFHAHKKNIDASGNVLHDDIG
                     IFLKNQIQDYFKGKNMPVSVKYIDPSYIIRSVPANANDSKFCVQLTYKAIHAAMAGRT
                     DFVVGYWNGAFTLLPIEAATQERKKINTEGEFWWNVLEATGQAMYMKKLKD"
BASE COUNT          647 a          307 c          335 g          533 t
ORIGIN      
        1 ccaaccaact taaaactatc tcaccacaga ttacacagat tctcacagat tctttaactt
       61 atggttcgat gaatgggttg ctgagcatgt tgaagtactt accaaccaac ttaaaactat
      121 ctcaccacag attacacaga ttctcacaga ttctttaact tatggttcga tgaatgggtt
      181 gctgagcatg ttgaagtact taccaaccaa ctataaactc atatctaata tctaatatct
      241 tatatctaat ctaatatctc atatctcata attaaaaatt accccatttt ttggttatcc
      301 ctaaactttt gtatataaaa aatcaaaaaa tataatataa actgtatttt tacttaaaat
      361 ttaaatgaga tatggacttt acaatatcac aattaggcaa atgtacggtg cgtacacctc
      421 ttcaactttc gaaaacaaaa aacgacggca tttacgacta tatagaagat acagaacgaa
      481 tactgttttc taattcgtta cacatagttg cgcatgaatg taaaaacaat gtaatgcctg
      541 cttcgtttga aaaaccgggg cccaaagaat ttttatattt tgaaccggca aaaacaaaag
      601 ttgctatagt tacatgcggt ggtttgtgtc cggggttaaa taatgtaatc agaggtttgg
      661 taaatcattt gtgggaaatt tataaagtaa gaaaaatatt gggagtgcaa tatggatatg
      721 ccggtttgaa tccggaaaat gaattaccat ttttagaatt aaatcccgaa atagttgatg
      781 atattcacaa aaacggtggg tctatgttag gttcgtcgcg aggcaatcag ccggtagata
      841 ttatagttga tactttggaa cgacataata ttaatatttt gttttgtatt ggtggcgatg
      901 gaacgttacg aggagcacat gctattcacc aagaaattac tcgaagaggt cttaaaattt
      961 caatagcagg tatacccaaa acaattgaca atgacataaa ttttatagat aaatcatttg
     1021 gatttgaaac agcatttacc gcagctcaag aaataatact caatgctcat aatgaagcca
     1081 aaggcacaca gaatggcgtg gcacttataa aactaatggg gcgcgattcg gggtttattg
     1141 cagcaaatgc agctttggca gtccccgatg tgaattttgt attgataccc gaaatggaat
     1201 ttgatttaga cgataccgaa acacagaatg gatttttaaa tcatttagaa cgccgattga
     1261 tacgaaaaaa acatgcagta atagttgtag cagaaggtgc aggacaacat ttgtttcatg
     1321 ctcacaaaaa aaatatagat gcctcgggca atgtattaca cgatgatata ggtatatttc
     1381 taaaaaatca gatacaagac tattttaaag ggaaaaacat gcctgtttct gtaaagtata
     1441 tcgatccaag ttatataatt agaagtgttc ctgcaaatgc caacgacagt aagttttgtg
     1501 tgcaactcac atataaagcc attcatgccg ctatggccgg acgaaccgat tttgttgtgg
     1561 gatattggaa cggcgcattt accctattac ccattgaagc agcaacacaa gaacgtaaaa
     1621 aaataaatac cgaaggcgaa ttttggtgga atgtgttaga agcaaccgga caagctatgt
     1681 atatgaaaaa attgaaagat taatttacac tgtataattg gcacctcttt caccacaaag
     1741 ttcacagagg aacacagagt aaattaaact catggttcga tgaatggggt gctgagcatg
     1801 ttgaagtact taccaaccaa ct
//
LOCUS       DAOTOW010000498         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50065_length_1822_cov_3.375778, whole genome shotgun sequence.
ACCESSION   DAOTOW010000498 DAOTOW010000000
VERSION     DAOTOW010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50065_length_1822_cov_3.375778"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..709)
                     /locus_tag="P5243_08535"
     CDS             complement(65..709)
                     /locus_tag="P5243_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS19537.1"
                     /translation="MEPITIHIVDDHTLFREGLKFLLSTTQSIRCVGESENGLECLQS
                     ITKTKPHIVLMDIEMPQKDGIATTRELMQIYPDLKIIALSMYGYEHYYSQMIDAGARG
                     FLMKNSAFDDVQKAIHEVYKGNTYFSPEILDALIKNLYKKKDNKPTQTELTEREIEIL
                     YNICKGFSNAEIADTLCISKRTVDKHRENLLLKTESKNTAGLVIYAIKNGLFEV"
     gene            complement(711..1655)
                     /locus_tag="P5243_08540"
     CDS             complement(711..1655)
                     /locus_tag="P5243_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS19538.1"
                     /translation="MAILIALVLAIILQLGASVFAVSLIKRTKYNASWILISIGFTLM
                     AVRRVYDLIYVLQSEGALEKQSIISNWLSVIVSLLIFLGAFYIRKIFDLQDRIDKIRK
                     ENESRVFAAIIKTEERERQTFAKELHDGLGPILSSVKMALSAINQSIAGDTNTQIIHK
                     TNLAIDEAIITIKEISNKLSPHILTNFGLDKALRNFIDTVVINKNITVSFRSDIENKR
                     FDFTVETVLYRVVCELLANTLHHAQAQNISIFIQNTPQELTLIYTDDGIGFTPENTKN
                     SGMGLSNIQSRIASIHGVISIESSEGKGVQVFITVKHI"
BASE COUNT          613 a          278 c          246 g          685 t
ORIGIN      
        1 ttatcaaaat atcctaactg taaaactatt tattttgatg gtttttaaga taaagattag
       61 ctaattacac ctcaaacaac ccatttttaa tcgcataaat caccaaacct gcggtgtttt
      121 tcgattctgt tttaaggagc agattttcgc gatgcttgtc tacagtgcgt ttgcttatac
      181 acaaagtatc agcaatttca gcattcgaaa atcctttgca gatattatac agtatctcaa
      241 tttctcgttc ggtaagttcg gtttgagtag gtttgttatc ctttttttta tataaattct
      301 taattaacgc atcaagtatt tcgggcgaaa aatatgtgtt gcccttataa acctcatgaa
      361 tagctttttg aacatcatca aaagccgaat ttttcattaa aaatcctcgt gcaccggcat
      421 caatcatttg actgtaatag tgttcataac cgtacattga aagtgctatg atttttaaat
      481 caggatatat ttgcattaat tctcgagttg tggcaatacc atctttttgg ggcatttcta
      541 tatccattaa aactatatgt ggttttgttt tggtaataga ttgtaagcat tctaatccat
      601 tttctgattc tcccacacat cgaatacttt gtgttgtaga aagtaaaaat ttaagtcctt
      661 cgcgaaacag agtatggtcg tcaacaatat gtattgtaat aggttccaat ttatatatgt
      721 tttacagtaa taaatacttg tacacctttc ccttctgaac tttctattga aataacacca
      781 tgaatagatg ctatacgtga ttgaatattt gataacccca taccggaatt ttttgtattc
      841 tcaggggtaa aaccaatacc gtcgtcggta tatattagag tcaattcttg aggcgtgttt
      901 tgtataaaaa ttgaaatatt ttgtgcttga gcatgatgta atgtgtttgc taataattca
      961 caaactactc tatataaaac tgtttctact gtaaagtcaa atcgtttgtt ttcaatatca
     1021 gatctgaatg aaacagttat atttttattt ataacaacag tatcaataaa atttcgcaat
     1081 gctttatcta atccaaaatt agttagaata tgagggctta atttatttga aatttctttt
     1141 atagttataa tagcttcatc tattgctaaa tttgttttat gaataatttg agtatttgta
     1201 tcacctgcta tactttgatt aattgcactt aatgccattt ttaccgatga taaaattggt
     1261 ccaagtccat catgtaattc ttttgcaaac gtttgtcgtt cacgttcttc tgttttaata
     1321 attgcagcaa atactctact ttcgttttct tttcgaattt tgtctattct atcttgtaaa
     1381 tcaaaaattt tgcgtatgta aaatgctcct aaaaatatca acaacgaaac aattaccgac
     1441 aaccaattac ttatgataga ttgtttttct aaagcaccct cactttgtag tacataaatt
     1501 aaatcatata ctctacgtac agccataaga gtaaatccta tagaaattag tatccatgaa
     1561 gcattatatt tagttcgctt tattaaactt acagcaaata ccgatgctcc taattgtaat
     1621 ataattgcta atacaagagc tattaaaatt gccattatta ttattttaag aattcaaata
     1681 tacatgaata attttatatg aagaataagt attttttatg atttaggtaa ttatagaacc
     1741 atctttttta aagtattaaa aattaaagag ttaaaaaaaa tataaaaaaa ataaaaaaaa
     1801 aagtaacaaa caatttggaa aa
//
LOCUS       DAOTOW010000499         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50077_length_1822_cov_2.768534, whole genome shotgun sequence.
ACCESSION   DAOTOW010000499 DAOTOW010000000
VERSION     DAOTOW010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50077_length_1822_cov_2.768534"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>209
                     /locus_tag="P5243_08545"
     CDS             <1..>209
                     /locus_tag="P5243_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRS19539.1"
                     /translation="PFFMHKRAEGVLPITHMEMTRFNISLEEGVNMVLYAIEHGIGGE
                     IFVPKIPSYKIDVLARAIAPSCKLV"
     assembly_gap    210..219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <220..465
                     /locus_tag="P5243_08550"
     CDS             <220..465
                     /locus_tag="P5243_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HRS19540.1"
                     /translation="PGEKLHEEMITSSDSYNTIDAGTYYIILPTDANKDLYLSTYKAK
                     PVTPGFTYNSGENDMWLDEEAIRKLIVKHVDPLFKPL"
     gene            477..1622
                     /gene="pseC"
                     /locus_tag="P5243_08555"
     CDS             477..1622
                     /gene="pseC"
                     /locus_tag="P5243_08555"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HRS19541.1"
                     /translation="MIPYGKQHITDDDIAAVIETLQSDFLTQGPKIAEFEQAFAAYVG
                     SSYAVAVNNGTAALHLGVLALGLQPHHKVITTPITFAASANCIQFCGAQVLFADIDPH
                     TFLIDINQVEALCQTHKDVVGIIPVDFAGYPVHMEQLRSVAQKYGLWILEDACHAPGA
                     WFTDSQGTTQLCGNGVFADATVFSFHPVKHIATGEGGMITTNNKELYEKLLMLRTHGI
                     TRNPELLHENHGGWYYEMQELGYNYRITDFQCALGISQLKRAQASLLRRNQIAHHYTQ
                     AFAQCKHITTPFVAADVFHAYHLYVIRVPNRKELYDYLRTKDIFTQVHYIPVHTMPYY
                     KAQGYKQGDFPIAEAYYATCLSLPMYPSLQQHEQDFVISTILDFYTS"
     gene            1625..>1822
                     /locus_tag="P5243_08560"
     CDS             1625..>1822
                     /locus_tag="P5243_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19542.1"
                     /translation="MHTEQIDFWTGTFGKEYTDRNTRDQQEWDSFFINYFGCTKLEIN
                     NRCFGHLPKDAKILEVGTNTGM"
BASE COUNT          558 a          370 c          368 g          516 t
ORIGIN      
        1 tcccattttt tatgcataaa cgtgccgaag gtgtgttgcc tataactcat atggaaatga
       61 cgcgcttcaa tatttcgctc gaagaaggtg taaatatggt gctgtatgct atagaacatg
      121 gtataggagg tgaaatattt gttcccaaaa ttccatcgta caaaattgat gtgcttgctc
      181 gtgctatagc accaagctgt aaattggtgn nnnnnnnnnc cgggagaaaa attgcacgaa
      241 gaaatgataa cttcaagcga ttcttacaat accattgatg ccggcacata ttacataatt
      301 ttaccaaccg atgccaacaa agatttgtat ctaagcacct ataaggcaaa accggtaact
      361 cccggattta cctacaattc gggcgaaaat gatatgtggc tcgacgaaga agcaattcgc
      421 aagcttattg taaaacatgt agatccattg tttaagcctt tataacagtt gcggcaatga
      481 ttccttacgg taaacaacat ataaccgacg acgatatagc tgctgtaata gaaacattgc
      541 agtcagattt tttgacgcaa ggtcctaaaa ttgccgaatt tgaacaagct tttgctgcgt
      601 atgttggcag ttcgtatgcc gtagccgtaa acaacggtac tgctgcattg caccttggtg
      661 tattggcttt ggggttacaa ccgcatcaca aggttataac tactcctatt acatttgctg
      721 caagtgccaa ctgtattcag ttttgcggtg ctcaggtgtt gtttgccgat attgacccgc
      781 acacattcct cattgatata aaccaggttg aagctttgtg tcaaacacac aaagatgtag
      841 ttggtataat tcccgtagat tttgcaggat acccggtaca catggaacaa ttgcgttcag
      901 tagcacaaaa atacggattg tggattttag aagatgcctg tcatgctccc ggtgcttggt
      961 tcaccgattc gcaaggcaca actcaattgt gcgggaatgg cgtatttgcc gacgctacgg
     1021 tattttcgtt tcacccggtt aagcatattg caacgggcga aggcggtatg ataactacca
     1081 acaataaaga actctacgaa aaactgttga tgcttcgtac gcacggaatt acacgcaatc
     1141 cggaactatt acacgaaaat cacggaggct ggtattacga aatgcaggaa ttaggatata
     1201 attaccgtat taccgatttt caatgtgcat tgggtattag tcaacttaag cgtgcgcagg
     1261 cttcgttgtt gcgccgtaac caaatagcac accactatac acaagcattt gcacagtgta
     1321 aacacattac aactccattt gttgctgccg atgtgtttca tgcttatcat ttatatgtaa
     1381 ttcgggtgcc aaatcgtaag gaattgtatg attatttgcg tacaaaagat atttttacac
     1441 aagtacacta tattcccgta catactatgc cctactacaa agctcaaggg tacaaacaag
     1501 gcgatttccc catagccgaa gcatattacg ccacatgctt aagtttgcct atgtacccta
     1561 gtttgcagca acacgaacaa gattttgtaa tttcaacaat tctcgatttt tatactagct
     1621 aattatgcat acagaacaaa tagatttttg gacaggaaca tttggaaaag aatacaccga
     1681 ccgtaatacg cgcgaccaac aagaatggga ttcatttttt ataaactatt ttggttgtac
     1741 caaattagag ataaacaatc gttgtttcgg acatctgcct aaagacgcaa aaatattgga
     1801 agtaggaacc aacaccggca tg
//
LOCUS       DAOTOW010000500         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50158_length_1820_cov_2.619263, whole genome shotgun sequence.
ACCESSION   DAOTOW010000500 DAOTOW010000000
VERSION     DAOTOW010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50158_length_1820_cov_2.619263"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1820
                     /locus_tag="P5243_08565"
     CDS             <1..>1820
                     /locus_tag="P5243_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS19543.1"
                     /translation="VVGFAPTGGTSAVHPHHRNVYLIDDIMANQNTMSIPTKNRQHGY
                     MIGGSRTVPFTESATNYQTSEGGIDYNAGLVGAIAYIVSRVAPVDTNKFGHPTPDLGP
                     DQSICGLSSIVLDSKITPDTKKTFTWKKDGTTVVNASTTARTYTATSAGTYQCVIDSA
                     GKWSTSDEIIITSVLPNVALGADKNLCNPSWDTLKTAASGAGITYSWKKDNVTIPGAT
                     ASSYIVYNPGTYICTISATGCTSKSDTIIVTSNLPVVQHDTICAAGTANLQVITTGTY
                     AWYDVLTGGTSLASGSSYSPNITATKTYYVQDASSVNGTVGPTALTSTTTSNWGVSNT
                     LQMAFTVGSSFTVNSIKMAVNSIYSAGSGTVTIEILDGSGNAFSPAKKFTSNSVSMTT
                     AMAGTLVEFPFTGFNIDKAWGTNLRIRVSAVTFNGALGFNESGAAYPYNSTPSGVMTI
                     TGAYNGTESKPTWYLYFYNWKISAGSTCARTPVRAVIEATNPKCTADTQAPTTPGAIT
                     FSAITQNSFTATWTASTDNIGVTGYEVLLNGTLYSTVTTNSISITSLTCNTTYTVRVR
                     AKDAAGNVSAYNTAASTTTTGVAAPTITNTTPTLCAGGTI"
BASE COUNT          598 a          359 c          338 g          525 t
ORIGIN      
        1 tgtggtaggt tttgctccta caggaggaac aagcgcagtg catccacatc atagaaatgt
       61 gtacttaatt gatgacatca tggccaacca aaatactatg tcaattccaa caaaaaacag
      121 acagcatgga tatatgattg gtggtagtag aactgttcct tttactgaaa gtgcaactaa
      181 ttatcaaaca tctgaaggtg gtattgatta taatgcggga ttagtaggag ctatagctta
      241 tattgtttct cgagttgctc cggttgatac caataaattt ggacacccaa cacctgattt
      301 aggccccgat caatctattt gtggtttaag cagcattgtt cttgactcta aaataacacc
      361 tgatactaaa aaaactttta cttggaaaaa agatggaact accgtggtaa atgcttctac
      421 taccgcacgc acctacacag caacatcggc aggaacctat caatgtgtaa ttgactcagc
      481 tggtaaatgg agcacatcgg acgaaatcat tattacatcg gtactaccaa atgtagcatt
      541 aggagccgac aaaaatttat gtaacccatc ttgggatact cttaaaactg ctgcatcagg
      601 tgcaggtata acttattctt ggaaaaaaga caatgttacc attccgggag ctactgcttc
      661 atcatatatt gtttacaatc caggaacata tatttgtaca attagtgcaa caggctgtac
      721 ctctaaatct gatacaatta tagtaacatc aaatttacct gttgtacaac atgatacaat
      781 ttgcgctgca ggaactgcaa atttacaagt tattacaacc ggtacttatg catggtatga
      841 tgtattaaca ggcggaacat cgcttgcttc gggctcaagt tattcaccaa atattactgc
      901 aactaaaaca tattatgtgc aagatgcttc atcggttaat ggtaccgttg ggccaactgc
      961 attaacatct acaacaacaa gcaattgggg tgttagtaat actttacaaa tggcatttac
     1021 cgtagggtca tcattcacag taaattctat aaaaatggct gtaaacagca tttatagtgc
     1081 cgggtcgggt actgtaacta tagaaatttt agatggaagt ggaaatgctt tttcacctgc
     1141 taaaaaattt acatcaaatt ctgtttcaat gactactgct atggccggaa cattagttga
     1201 atttcctttt accgggttta atattgacaa agcttggggt acaaatttac gaatacgcgt
     1261 ttctgcagta acgtttaatg gagcattagg atttaacgaa agcggcgcag catatccata
     1321 taattcaaca ccttcgggag taatgacaat aacaggagca tacaacggaa cagaatcaaa
     1381 accaacatgg tatttatatt tttacaattg gaaaatttcg gcaggttcaa catgtgctcg
     1441 tacaccggta cgtgcggtaa tagaagcaac caatcctaaa tgtacggcag atacacaagc
     1501 acctacaact ccgggagcaa ttacgtttag tgctattaca caaaattcat ttaccgctac
     1561 ttggacagcc tctaccgata acattggtgt aaccggttat gaagtattac ttaatggcac
     1621 attatattct actgttacta caaattcaat ttctataaca agtttaacat gtaatacaac
     1681 atatacagtt cgagttagag caaaagatgc tgcaggtaat gtatcggcat acaatacagc
     1741 agcttctaca actactaccg gggttgctgc accaactatt accaatacaa ctcctaccct
     1801 ttgcgcaggt ggcactattt
//
LOCUS       DAOTOW010000501         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50228_length_1818_cov_4.361883, whole genome shotgun sequence.
ACCESSION   DAOTOW010000501 DAOTOW010000000
VERSION     DAOTOW010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50228_length_1818_cov_4.361883"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="P5243_08570"
     CDS             <1..317
                     /locus_tag="P5243_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19544.1"
                     /translation="LTCKYIDGIFEYKSFNAKECFYDDWLIQIPSIDSMLYCVFPNPF
                     TNTITIHGMTEGMRYTLYTSIGTPVLSGSNATIDTKNIPKGVYMLEVIGAKGTQVVRV
                     VK"
     gene            complement(353..1129)
                     /locus_tag="P5243_08575"
     CDS             complement(353..1129)
                     /locus_tag="P5243_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19545.1"
                     /translation="MNFQLSDKKIYQKLGYSIDQEGILTRFLREGDAWKSHLEHTKQW
                     IRDAYAHKGYSSIAIFGSGWLLDVPIDDIIRDFKEVYLFDIIHPLQITHKFRKYPHVH
                     FIEADVTGGVAKKIYERITAKSHVRKQPLPQLTQTQFSYDIPFDLVVSLNIVNQLDIL
                     LLEALEKHETVSEEEKQDFRYCIQNNHMHLLEQFNYCLISDWEKLVTHTKTGHTHSEP
                     LVFVEPPAHTHHSEWLWNFDSHGLYEENSKIEFLVRAFRI"
     gene            complement(1252..>1818)
                     /gene="proB"
                     /locus_tag="P5243_08580"
     CDS             complement(1252..>1818)
                     /gene="proB"
                     /locus_tag="P5243_08580"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRS19546.1"
                     /translation="AAAAIGQAVLAKIYRKFFDTYNMHVGQVLLTYDVITDKQKHINA
                     ENTFAKLLELGAIPIVNENDTVATDEIEIGDNDRLSAMVASICKADLLIMLSDIDGFF
                     DSDPRKNPQAKRIPIITTVDEKISASAGSAGSSFGTGGMSTKITAVQICHADNIDTVI
                     ANADEPEILYNILQGHDLGTLFVAPNNV"
BASE COUNT          560 a          317 c          323 g          618 t
ORIGIN      
        1 atttaacatg taaatatata gatggaattt tcgaatataa atcatttaat gccaaagaat
       61 gtttttatga tgattggctt atacaaattc caagtattga ttcaatgcta tattgtgtat
      121 ttcccaaccc atttaccaat accattacaa ttcatggtat gaccgaagga atgcgatata
      181 ctctgtacac aagtataggt acacctgtat tgagtggttc gaatgcaact atagatacta
      241 aaaatatacc caagggtgta tatatgctcg aagttatagg tgctaagggc acgcaagtag
      301 tgcgagtggt gaagtgaaag tattaaaata cagtaatagg cataaatata cgttatattc
      361 gaaacgcccg cactagaaat tcaattttgc tattttcttc gtacaagccg tgtgagtcaa
      421 agttccacag ccattcgcta tggtgtgtgt gtgcaggtgg ttctacaaat accaatggtt
      481 cggagtgtgt gtgcccggtt ttagtatgag taactaattt ctcccaatcg cttattaagc
      541 agtagttgaa ttgctccagc aaatgcatat gattgttttg tatacaatat ctaaaatcct
      601 gtttttcttc ctcgctcacc gtttcgtgtt tttccaaagc ttcgagcaat agtatatcaa
      661 gctggtttac tatattgagc gatactacaa ggtcaaaagg tatatcatac gaaaattgtg
      721 tttgcgttaa ttgtggtaag ggctgtttac gaacatgaga ttttgccgta atccgttcgt
      781 atattttttt tgctacccct ccggttacat cggcttctat aaagtgtaca tgcgggtatt
      841 ttctgaattt atgggtaatt tgtaaaggat gtatgatatc gaacaggtat acctctttga
      901 aatcgcgaat aatatcgtct attggtacat cgagtaacca tccgctgcca aatattgcta
      961 tagaagaata gcctttgtga gcatacgcat cgcgaatcca ttgtttggta tgttctaagt
     1021 gtgatttcca cgcgtcgcct tcgcgtaaaa aacgggtaag aataccttct tggtcaatgc
     1081 tatatcctaa tttttgatat atttttttgt ctgataattg aaaattcaca gaattgaatt
     1141 aatatgtcgg attataactg tttaaaatat tattatagat taatacaata tatcattttt
     1201 ctaaatctga cttgttgctt aaagatacag taatgactat cacaaaactc ttcacacatt
     1261 gttaggagct acaaaaagag ttcctaaatc atgtccttgc aaaatattat ataaaatttc
     1321 tggttcatcg gcatttgcaa ttaccgtatc tatattatcg gcatgacaaa tttgaacagc
     1381 tgtaattttg gtggacatac cacctgttcc aaagcttgaa cctgcagaac ctgcactagc
     1441 cgaaattttt tcatcaactg tggtaattat tgggattcgc tttgcttgtg ggtttttgcg
     1501 cggatctgaa tcaaaaaaac catcaatatc agaaagcatt attaataaat cggctttaca
     1561 aatagatgca accattgcag aaagcctatc attgtcgcct atttcaattt catcggttgc
     1621 cacagtatca ttttcattta caattggaat ggcacctaat tcaagtaatt tagcaaatgt
     1681 attttcagca ttaatgtgtt tttgtttatc tgttataaca tcataggtta acaatacttg
     1741 ccctacatgc atattatacg tatcaaaaaa cttacgataa attttagcta aaacggcctg
     1801 tccaatagcc gcagcagc
//
LOCUS       DAOTOW010000502         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50234_length_1818_cov_3.509926, whole genome shotgun sequence.
ACCESSION   DAOTOW010000502 DAOTOW010000000
VERSION     DAOTOW010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50234_length_1818_cov_3.509926"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="P5243_08585"
     CDS             complement(<1..1172)
                     /locus_tag="P5243_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS19547.1"
                     /translation="MKRLLIYILISYSFTTYAQNGVITGRVYNELTNEPVAFAYVEIT
                     GQPLIGALTDLDGKFIITGLPAGIVTVTASFIGFKTVISRDIQVTNAKQAYVEIAMQK
                     DVQMLEGVVVKASPFIKPEESPLSLQRIGLQEIDANPGSNRDISKVIQSFPGVGSTVS
                     FRNDIIIRGGGPNESRFYLDGIEIPNLNHFSTQGSSGGPVGIINADFIGGVDFYSGAF
                     PAMYGNALSGVFDFSQISGNKEKTKFRGSVGASELSATLDGPSGDNSTYIASVRRSYL
                     QFLFDAIGLPFLPTFTDYQIKHKTLFPDRSELTIVSIGALDDFSLNLGLENPDESQEY
                     ILAYLPVTKQWSYAIGANYKKFTDAGYHRYVVSRNMLNNISYKYPENNENLPRILD"
     gene            complement(1176..>1818)
                     /locus_tag="P5243_08590"
     CDS             complement(1176..>1818)
                     /locus_tag="P5243_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19548.1"
                     /translation="ILQTNQDLPFVLEFEAGKYFNNPTFAVWIEDMNETLVSTIFVTK
                     SIATGKYGHAYSKDNIWLPDSGISIRPATLPYWLHKQTNGNIHSVSPQNPLVPDAVSG
                     ATPKGSFTIETAVSKQLPNKFRVLLEINQAWDWNEYYTNAKYPGNFEYKTSCQPSVIY
                     AVTIDLQSPIKTYYLQPIGHGHYAGEDGLLYTDLRGHTTALQIAKSISVQLKK"
BASE COUNT          582 a          341 c          272 g          623 t
ORIGIN      
        1 taatccaata ttcgcggtaa attttcatta ttttcaggat atttatatga tatattatta
       61 agcatatttc tacttactac atatctgtgg tatcccgcat cggtaaattt tttataattt
      121 gctcctattg cataactcca ttgctttgtt accggcaaat aagctaaaat atattcttgt
      181 gattcatctg gattttccaa acctaaattt aaactaaaat catcaagagc accaatactt
      241 acaattgtta actcacttct atcggggaat agagttttat gttttatttg ataatcggta
      301 aacgttggta aaaacggtaa tccaattgca tcaaacaaaa attgaagata cgaacgacga
      361 acagacgcta tataggtaga attgtcacct gatggtccgt caagtgttgc cgatagctct
      421 gatgctccta cactccctct aaatttagtt ttttctttat tacctgaaat ctgactaaaa
      481 tcaaacacgc cactcaaagc attgccatac atagcaggaa aagcaccgga ataaaaatca
      541 acaccaccta taaaatctgc atttataata ccaacaggac caccacttga accttgagtt
      601 gaaaaatgat taaggtttgg tatttcaatt ccatcaagat aaaatctact ttcattaggt
      661 ccaccaccac gaattataat gtcatttcta aaagaaactg tagaacctac tcccgggaac
      721 gattgtataa cctttgaaat atccctgtta cttccaggat ttgcatcaat ttcttgtaat
      781 cctattcgtt gcaaagacaa tggactttct tcgggtttaa taaaaggcga tgcctttaca
      841 acaacacctt caagcatttg aacatctttt tgcattgcaa tttccacata cgcctgcttt
      901 gcatttgtta cttgtatatc ccttgaaatc acagttttaa aaccaataaa acttgcagtt
      961 actgttacaa ttcctgcggg taatccggtt ataataaatt taccatctaa atcggtcaaa
     1021 gcacctatga gtggttgtcc tgtaatttct acatatgcaa aagctacagg ttcattggtt
     1081 aattcattgt atacacggcc tgttattacg ccattttgag catatgtagt aaatgaataa
     1141 cttattaata tatatatcaa caatcgtttc atatgttatt tttttaattg tacagaaatt
     1201 gattttgcta tttgtaatgc agttgtatgt ccacgcaaat cagtatataa taatccatct
     1261 tcacctgcat aatggccatg accaattggt tgtaaataat atgttttaat tggtgattgt
     1321 aaatctatag ttactgcata tattactgaa ggttgacatg aggttttata ttcaaaattt
     1381 cccggatatt ttgcattagt atagtattca ttccaatccc aagcttgatt tatttctaaa
     1441 agtacacgaa acttatttgg caactgttta gataccgctg tttctattgt aaaacttcct
     1501 tttggtgttg ctccgctcac agcatcagga actaaaggat tttgcggcga tacagaatga
     1561 atgttgccat ttgtctgttt atgcaaccaa taaggcaaag ttgcggggcg aattgatatt
     1621 cccgaatcag gtaaccatat attatcttta ctatatgcat gaccatactt ccctgttgca
     1681 attgatttag taacaaaaat agtagatacc aaggtttcgt tcatatcttc tatccacacc
     1741 gcaaaagtag gattattaaa atatttaccc gcttcaaatt ctaaaacaaa cggaagatct
     1801 tgattggttt gtaatatc
//
LOCUS       DAOTOW010000503         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50317_length_1816_cov_3.079500, whole genome shotgun sequence.
ACCESSION   DAOTOW010000503 DAOTOW010000000
VERSION     DAOTOW010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50317_length_1816_cov_3.079500"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="P5243_08595"
     CDS             <1..1599
                     /locus_tag="P5243_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS19549.1"
                     /translation="LPLGLNFQQIDHNFGSAPYFREWLRMTISANKPNRNNYWSYELY
                     KRDSSQWVNNPLYGWCNKNEKSPDTFYNIYKDGLKIYTTIDSRMQEYAEQAMIKHMKE
                     IVQPAYDSENKNNPKSPFSSNISQEEFQSRIKAAIIQSERYKSIPNAKEKDWNSIMAI
                     FKKPVKMTVFSWHGEIDTIMSPLDSIKYMKRFLQCGLLSMEAKTGHVKAFVGGINYKY
                     FQYDHIMQQRRQVGSTFKPFLYTLAMQEGMSPCKEFANVPTSFYLADGTQWTPDNSDD
                     VRKGQMVSLAWALATSNNWISAKLMQLHKPEAVIALAREMGLYNPIDPVPSICLGVAD
                     LTLHEMVGAFATFPNKGVYTEPIFVTRIEDKFGKVISTFTPKQNEAISEETAYLMVNL
                     LQNVVNMVDREAKAYGTAISLRTRYDIRIPLGGKTGTTNDHADGWFIGFSPELVTGVW
                     VGGDERSIRFRSFRYGQGARMAMPMFAYYMKSLIADSTNLGYHFMKDFDKPANINYSK
                     INCEKYGDPTQIPIKQSVNYDEFY"
     gene            1603..>1816
                     /locus_tag="P5243_08600"
     CDS             1603..>1816
                     /locus_tag="P5243_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS19550.1"
                     /translation="MDESVFVCKEFSIVQNSSVLKLGTDAIILGCLTDFYNPSHILDI
                     GTGTGILTLMMAQKYHAQFTAIDISEE"
BASE COUNT          653 a          301 c          316 g          546 t
ORIGIN      
        1 ttacctctcg gattaaactt tcaacaaatt gatcacaact ttggttcagc gccttacttt
       61 agagaatggc ttagaatgac aattagtgcc aacaaaccta acagaaataa ttactggagc
      121 tacgaattat acaaaagaga ctcatcacaa tgggttaaca atccattata tgggtggtgt
      181 aataaaaatg aaaaatcacc cgataccttt tataatatat ataaagatgg tcttaaaata
      241 tatactacca tagattctcg tatgcaagaa tatgcagaac aagcaatgat aaaacacatg
      301 aaagaaattg ttcaaccggc atatgattcc gaaaataaaa ataatccaaa atcaccgttt
      361 tcatctaata tttctcaaga agagttccaa agtagaataa aagcggctat tattcaaagt
      421 gaacgttata aaagtattcc taatgcaaaa gaaaaagatt ggaattcaat aatggctata
      481 tttaaaaaac ctgtaaaaat gaccgttttt tcatggcatg gagaaataga tacaataatg
      541 tctccattag attcaattaa atatatgaaa cggtttttac aatgtggtct tctttctatg
      601 gaagcaaaaa ccggtcacgt aaaagcattc gttggaggca ttaattataa atatttccaa
      661 tatgaccata ttatgcagca acgtcgtcaa gttggttcta cattcaaacc atttttatat
      721 accctcgcta tgcaagaagg tatgtcgcca tgtaaagaat ttgcaaatgt acctacatca
      781 ttttatttag ccgatggtac tcaatggaca ccggacaaca gcgatgatgt aagaaaagga
      841 caaatggtaa gtttagcttg ggcattagca acttctaaca actggatatc ggcaaaactt
      901 atgcaattac ataaacctga agcagtaata gcattggctc gcgaaatggg attatataat
      961 ccaatagatc cggttccttc tatttgttta ggagttgccg atttaacatt acatgaaatg
     1021 gttggagcat ttgctacgtt cccaaacaaa ggagtgtata ctgagcctat ttttgtaact
     1081 cgtatagaag ataaattcgg aaaagttatt tctacattta ctcctaaaca aaatgaagct
     1141 ataagcgaag aaactgcata ccttatggta aacttactac aaaatgtagt aaatatggta
     1201 gatagagaag caaaagcata tggtactgcc atatcattac gaactcgcta cgacattcgt
     1261 attccacttg gaggcaaaac cggaacaact aatgatcatg ctgatggttg gtttattggc
     1321 ttttctccgg aacttgtaac aggagtatgg gttggtggtg acgagcgttc tattcgattt
     1381 agaagtttcc gctacggaca aggtgctcgt atggctatgc ctatgtttgc atattatatg
     1441 aaaagcctca ttgctgacag tacaaattta gggtatcatt tcatgaaaga ttttgataaa
     1501 cctgcaaata ttaattattc aaaaataaat tgtgaaaaat acggcgaccc gactcaaata
     1561 ccaatcaaac aatcagtaaa ttacgacgaa ttttactaac aaatggatga atcagtcttt
     1621 gtttgtaaag aattttcaat agttcaaaat tcatctgttc tcaaattggg aacagatgca
     1681 attatattag ggtgcttaac agatttttat aatccttctc atatacttga tattggaaca
     1741 ggtacaggaa ttttgactct tatgatggct cagaaatatc atgcacaatt tactgctatt
     1801 gacatttcag aagaat
//
LOCUS       DAOTOW010000504         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50358_length_1815_cov_3.107955, whole genome shotgun sequence.
ACCESSION   DAOTOW010000504 DAOTOW010000000
VERSION     DAOTOW010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50358_length_1815_cov_3.107955"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..725
                     /locus_tag="P5243_08605"
     CDS             120..725
                     /locus_tag="P5243_08605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRS19551.1"
                     /translation="MQTFPEIRSVDSADAEHLPLTSFEGKIHIITSIEQVHAAVKYLQ
                     TCSKLGFDTETRPSFKKGLLYPLSLLQLSTESHAFLFRLQYCGLPKELCDILASPSII
                     KVGAAIHDDVAALQKITKFKPQGFIDLQTIAKQLEIENFGLKKLAQLVLGIRISKRQQ
                     LSNWDSQKLTESQCLYAATDAWVSLEIYKKLETFLHDTTIS"
     gene            706..>1815
                     /locus_tag="P5243_08610"
     CDS             706..>1815
                     /locus_tag="P5243_08610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRS19552.1"
                     /translation="MIQLYLKPGKEQSVKRLHPWIFSGAIASYSGNPQEGDIVSVFSS
                     TKEFLALGHYQKSSIAVRILSFSQEFIHQEFWNKRIASAFEYRKTIGLVDNLQTNCYR
                     LVHGEGDNLPGLIIDFYNGVAVLQMHSVGMYKHMNNIVEACKTVLGERLQAVYNKSTS
                     TLPFKAETDNVDGLVWGNVHTTQVHEYGNTFVIDWEKGQKTGFFIDQRENRKLVELYS
                     KDKRVLNTFCYTGGFSVYALRGGAHQVVSVDSSLRAIELTNEAIELNFPLCSNHIAMT
                     EDVFDFFQTNTQKFDVIILDPPAFAKHNSALNNALQGYKRLNMKAMEHLAPGGILFTF
                     SCSQVVTKEDFRKSVFAAAANCKRSVRIIQQLTQPA"
BASE COUNT          643 a          257 c          331 g          584 t
ORIGIN      
        1 agtgtatgag tgtttacaaa attttggaat gtattggggg ccacgtgtat tatcaattat
       61 gcaagaattt catgtattgt tatattctgt tgattctgaa atagataatg aagagtaaca
      121 tgcaaacgtt tccggaaata cggtcagtag atagtgccga tgctgaacat ttaccattaa
      181 cttcatttga gggaaaaatc catataatta cgtcaataga acaagtacat gctgccgtaa
      241 agtatttgca aacctgttca aaattaggat ttgatacaga aacaagacca tcatttaaga
      301 aaggtttact gtatcctttg tccttattac aattaagtac agagtctcat gcatttttat
      361 ttcgattaca gtattgcggt ttgccaaaag aattgtgtga tatattagct tcaccatcta
      421 ttataaaagt aggagcagct atacatgatg atgttgcagc attacaaaaa attacaaaat
      481 ttaaacctca ggggtttatt gatttacaaa ctattgcaaa gcaattggaa attgaaaact
      541 ttggattaaa aaagttagct caattagtat taggtattcg tatttctaaa cgacaacaat
      601 tatcaaattg ggattctcaa aaacttaccg aatctcaatg tctgtatgct gctaccgatg
      661 cgtgggtaag tttggaaatt tataaaaaat tagaaacttt tttacatgat acaactatat
      721 cttaaaccag gaaaagagca aagtgtaaaa cgtttgcatc catggatttt ttcaggagct
      781 attgcttcat attcaggaaa tcctcaagaa ggcgatattg tctctgtatt ttcaagtacc
      841 aaagaatttt tggccttagg gcattatcaa aaatcatcta ttgccgtacg aattttaagt
      901 ttttcacaag aatttattca tcaagaattt tggaacaaac gtattgcttc agcttttgaa
      961 tatagaaaaa ctataggatt ggttgataat ttgcaaacaa attgttatag gttagtacat
     1021 ggtgaaggag ataacttgcc gggacttatt atagatttct ataatggagt agcagtattg
     1081 caaatgcatt cagtaggcat gtataaacat atgaataata tagtagaagc atgtaaaaca
     1141 gtacttggtg aaagattgca agctgtatat aataagagta catcaacatt accatttaaa
     1201 gcagaaacag ataatgttga cggcttagtt tggggtaatg tacatactac acaagtacac
     1261 gaatacggaa atacatttgt aattgattgg gaaaagggac aaaaaacggg tttttttata
     1321 gatcaacgtg aaaatagaaa gttagttgaa ttatattcaa aagataagcg agttttaaat
     1381 acattttgtt ataccggagg attctctgta tacgcattgc gtggtggagc acatcaagta
     1441 gtatctgttg acagttcttt acgtgcaata gaattaacca acgaggcaat agagcttaat
     1501 tttccattat gtagtaatca tattgctatg actgaagatg tttttgattt ttttcaaaca
     1561 aatacacaaa aatttgatgt aattatactt gaccctccgg catttgcaaa acacaatagt
     1621 gctttgaata atgcattgca aggatataag agattaaata tgaaagcaat ggaacattta
     1681 gctcccggtg gaatattgtt tacattttca tgttcacaag tagttacaaa agaagatttc
     1741 agaaaatcgg tttttgctgc agcagccaat tgtaaacgtt cagtacgaat tattcaacaa
     1801 ttgactcaac cagcc
//
LOCUS       DAOTOW010000505         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50612_length_1809_cov_3.030217, whole genome shotgun sequence.
ACCESSION   DAOTOW010000505 DAOTOW010000000
VERSION     DAOTOW010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50612_length_1809_cov_3.030217"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="P5243_08615"
     CDS             <1..1523
                     /locus_tag="P5243_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS19553.1"
                     /translation="VAADVYKKKQTAAQKMRIPIERIHDMRITKRSIDARSYNIKVNL
                     ELQCAIDQTKQLPKPHLFFKEQDVTHKPQVHIIGTGPAGLFAALRLIELGYKPILFER
                     GKDVSARKRDIALLNRNESLHPDSNYCFGEGGAGTFSDGKLYTRSKKKGDNQRVLELF
                     VLHGASPNILVDSHPHLGTDKLPDIITNIRNTICNLGGEIHFNAKITNITVRNNRITH
                     IECNAMQIAVDTLILATGHSARDIYYMLHNKGIELEAKPCAMGVRAEHPQELIDRIQY
                     HGNALDVLPAASYNLVHQVADRGVYSFCMCPGGIIVPSATDSEQIVVNGMSNSQRNSP
                     FANSGIAVEMRVEDFADFAEYGVLAGLKYQEYLETLAFRNGGHNQIAPAQGIRDFIQK
                     RLSKQLPECSYVPGVVSSPLHFWLPQHISERLREAFTAFDRKMKGYAGADGIIVGVES
                     RTSSPVRIPRNPETFTHVRINNLYPCGEGSGYAGGIVSSAIDGINCAEKCVLTTNK"
     gene            complement(1583..>1809)
                     /locus_tag="P5243_08620"
     CDS             complement(1583..>1809)
                     /locus_tag="P5243_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS19554.1"
                     /translation="VWVYDKWGNLVWYSDEVENGIFVGKWDGTYKGELLQSDTYIWKM
                     EAKLLDGKQWEGLQLSNGKFVKYGSVTLIR"
BASE COUNT          611 a          299 c          329 g          570 t
ORIGIN      
        1 atgtagctgc cgatgtttat aaaaaaaagc aaactgctgc acaaaaaatg agaattccca
       61 tagagcggat tcatgacatg cgaattacaa aacgttccat agatgcccgt tcatataata
      121 taaaggttaa tttagaattg caatgcgcaa ttgatcaaac caaacaattg cctaaaccgc
      181 atttgttttt taaagaacaa gatgtaacgc ataaacctca ggtacatatc ataggaacag
      241 gtcctgcggg tttatttgca gcattacggt taatagaatt agggtataaa cctatacttt
      301 ttgaacgagg taaagatgtg agtgctcgta aacgtgatat tgctctactt aatagaaatg
      361 agtctttaca tcctgattca aattattgtt ttggagaggg tggagcagga acattttctg
      421 atggtaaatt atatactcgt tcaaaaaaga aaggtgataa ccagcgcgta ttagaattat
      481 ttgtattaca tggagcttca cccaatatac ttgttgattc acatccgcat ttaggaaccg
      541 ataaattgcc tgatattatt acaaatattc gtaatacaat atgcaatttg ggtggagaaa
      601 ttcatttcaa tgcaaaaatt acaaatataa cagtacggaa taatagaata acacatatag
      661 aatgcaatgc aatgcaaatt gctgtagata cgttaattct tgctaccgga cattcggcac
      721 gtgatatata ttatatgtta cataataaag gtatagaatt agaggctaaa ccatgtgcca
      781 tgggagtacg cgctgaacac ccacaagaat taattgatag aattcaatat catggtaatg
      841 ctttagatgt gttacctgca gctagttata atttagttca tcaagtagct gatagaggcg
      901 tatattcatt ttgtatgtgt cccggaggta ttatagttcc atcagcaaca gattcagagc
      961 aaatagttgt aaatggtatg tcaaattcac agcgtaattc cccttttgca aattctggaa
     1021 tagctgtaga aatgagagta gaagattttg ccgattttgc agaatatggt gtgttggcag
     1081 gtttaaaata tcaagagtat ttagaaacat tggcttttcg aaacggaggg cataatcaaa
     1141 tagctcctgc tcaaggtatt cgtgacttta ttcaaaaacg tttatcaaaa cagttacctg
     1201 aatgttcgta tgtacccgga gtagttagtt ctccgcttca tttttggttg cctcaacata
     1261 tttcagaacg gttacgagaa gcatttacag cttttgatcg taaaatgaaa ggatatgcag
     1321 gagcagatgg aattattgta ggtgttgaat ctcgaacatc atcaccggta agaatacctc
     1381 gtaatccgga gacatttact catgtgcgca ttaataattt gtatccatgt ggagaaggat
     1441 caggatatgc aggtggtata gtttcatcgg ctatagatgg aataaattgt gcagaaaaat
     1501 gtgtattgac caccaataaa taaaaaaaga ggttgtttta ttactaaaac aacctctttt
     1561 tttgaatagt attcactagt aattaacgaa ttaaagtaac agaaccatat tttacaaatt
     1621 tgccattact taattgtaag ccttcccatt gttttccatc aagaagtttt gcttccattt
     1681 tccaaatata ggtatcagat tgtagtaatt ctcctttgta agttccatcc catttcccta
     1741 caaaaattcc attttctact tcatcactat accatactaa attaccccat ttatcatata
     1801 cccaaacct
//
LOCUS       DAOTOW010000506         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50649_length_1808_cov_2.816885, whole genome shotgun sequence.
ACCESSION   DAOTOW010000506 DAOTOW010000000
VERSION     DAOTOW010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50649_length_1808_cov_2.816885"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="P5243_08625"
     CDS             <1..408
                     /locus_tag="P5243_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19555.1"
                     /translation="SNLEATYNSQIMSLETYLRNLFGTDNIHIIDSPIRESSHVYNIE
                     EEVDEPYIYQAEELVVVNGDWFFKNDNDELIALHVNGDHWFFQTAEDEALSYNTIVKV
                     PAIFESDNEFAAKLQAALMQYIFSDKTYIIQYI"
     gene            405..1253
                     /locus_tag="P5243_08630"
     CDS             405..1253
                     /locus_tag="P5243_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19556.1"
                     /translation="MKKLETNHNGGLRLNWDDIRWSQNGIIDSLNSAVKAIANQTQVF
                     IMSGCDTRRWVGYGNQNGSFIDFELSSGVICINGELFNFPGFVAPGYNIPSTCKFWFV
                     IDIAYDPIGVKTDKYYQQEHQCYADRYVRIELGDSYPTGVSGIDYYPIVKHTIDNKPN
                     LMYLYEILEENLQKKTIQNFVTPLNNWLTQNLKFTQNGKLGSLSGHVTQGAGDEQTGT
                     YICQFSGSGNVNTYNYFTVRRYSSANVYKGECLMYVLGNKLYIENVSDASQGDIYMIG
                     ITFIMQ"
     gene            1255..>1808
                     /locus_tag="P5243_08635"
     CDS             1255..>1808
                     /locus_tag="P5243_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19557.1"
                     /translation="MAIKTPVELLQDISTYITNKVGKIRRSEHGAVERDIVESFDSLI
                     TAEKNARINADSLIMFNVGEAITELEEDLTASIGNKVSGPAISVNNSIPVFDGITGKL
                     LKETQFKIEGYEINGGLNGSIILNNSSLTNRTLSIQHGSYNDENAIKIGRNLATQHYI
                     SFANDTAGGCSNWISFKIWNNQDQ"
BASE COUNT          672 a          220 c          366 g          550 t
ORIGIN      
        1 tctaatttgg aggctacata caatagtcag ataatgagtt tagagacgta tttacgtaat
       61 ttgtttggca cagataacat acacataatt gatagtccta tacgcgaaag ctcgcacgtg
      121 tataatattg aagaagaggt agatgagcca tatatatatc aggcagaaga attggtagta
      181 gtaaatggtg attggttttt taaaaatgat aatgatgagt taatagcatt acatgttaac
      241 ggtgatcatt ggttttttca gacagcagaa gatgaagctt tgagttataa tacaatagtt
      301 aaagttccgg caatatttga gtcggacaat gagtttgctg cgaaattaca agcagcttta
      361 atgcaataca tatttagtga taaaacttat ataatacagt atatatgaaa aaattagaaa
      421 caaaccacaa tggtggttta cgcttaaatt gggacgatat tcgttggtct caaaatggta
      481 tcattgatag tttaaatagt gctgtgaaag caatagcaaa tcaaacgcaa gtttttatta
      541 tgagtggttg cgatacgaga cgttgggttg gctatggaaa tcaaaatggt agttttatag
      601 attttgaatt atcaagcggt gtaatatgta ttaatggaga attgtttaat tttcctggat
      661 ttgtagcacc cggttataat ataccaagca cttgcaagtt ttggtttgta attgatatcg
      721 cgtatgatcc aataggtgta aaaacagata aatattatca acaagaacat caatgttatg
      781 cggataggta tgttagaata gaattaggag acagttatcc aacaggagta tctgggatag
      841 attattatcc aatagtaaaa catacgattg ataataagcc aaatttaatg tatttgtatg
      901 aaatactaga agaaaatttg caaaaaaaaa caattcaaaa ttttgtaacc ccacttaata
      961 attggttgac tcaaaatttg aaatttactc aaaacggtaa acttggaagt ttaagtggtc
     1021 atgttacaca aggagcagga gacgaacaaa caggaacata tatttgtcaa ttttcaggaa
     1081 gtggaaatgt taatacatat aattatttta ctgttcgtag atatagttct gctaatgtat
     1141 ataaaggaga atgcttaatg tatgtattag gtaataagct atatatagaa aacgtgagtg
     1201 atgcgtctca aggtgatata tatatgatag gtataacatt tattatgcaa taaaatggca
     1261 ataaaaaccc cggtagaatt actgcaagac ataagtacgt atataaccaa caaggttggc
     1321 aagatacgta ggagcgaaca tggagcagtg gagcgtgata tagtggaatc atttgattcg
     1381 cttataactg ctgaaaaaaa cgcgaggata aatgctgata gccttattat gtttaatgta
     1441 ggagaagcaa ttactgaatt agaggaagat ttgacggcaa gtattgggaa taaagtaagc
     1501 ggaccagcta tttctgtcaa caattctata ccagtattcg atggtattac aggtaagtta
     1561 ttgaaagaaa cgcaattcaa aattgaaggt tatgaaataa atggtggctt gaatggttcc
     1621 ataattctta ataattcatc tttaactaat agaacattga gtatacagca tggttcttat
     1681 aacgatgaaa atgcgattaa aataggaaga aatctcgcaa cacaacacta tatatcattt
     1741 gcgaatgata cagcaggagg ttgttcaaat tggatttcat tcaaaatatg gaacaaccaa
     1801 gaccagag
//
LOCUS       DAOTOW010000507         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_50684_length_1807_cov_3.321918, whole genome shotgun sequence.
ACCESSION   DAOTOW010000507 DAOTOW010000000
VERSION     DAOTOW010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50684_length_1807_cov_3.321918"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="P5243_08640"
     CDS             complement(<1..916)
                     /locus_tag="P5243_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS19558.1"
                     /translation="MLSLKRLRLPFFKKNNYTLQCIIGAAISSNEQKKFNKDFSLTRY
                     VRAHVYVLAYKNIEIKDFFTIFKQTNQTDPKPIIEPVPVVMLEQEINSIRKNDRLFSF
                     KQFEVYCSSAVRIPFIIREIGRLRELTFRAVGEGTNNEIDIDEYDIYYEHLFIWDTTA
                     KKVVGAYRIGKGNEILKQYGKKGFYIHSLFKIQNTVLPILSQSLELGRSFIVPEYQKH
                     HHSLFLLWRGIVYFLLKNEEYRYLIGPVSISNSFTEISKSLIVSFIKSQHFHHTLAKQ
                     IRPRKDFKVPKKLVGDIQALVEHVDLKKL"
     gene            complement(910..1566)
                     /locus_tag="P5243_08645"
     CDS             complement(910..1566)
                     /locus_tag="P5243_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19559.1"
                     /translation="MIQSSFLSQSQIRKIGQLNRLKYQSMYHMFHSYCETMLQGTSVH
                     AIDDYFASHCIETFFKQLDIEIQYNQHMLEQIPATGPFAVIANQPLGGLDALLLYKII
                     SQKRPDFKIVEDIGAIQNPVFEKIFFQTYPFADSKGSPNKSGIIKMYEHFKNNQALGF
                     FPAQQGNIYKFNKQHKTDGEWQTNILGAVKYAEIPIIPVYIQHSDATMLEIAKKRIPC
                     "
BASE COUNT          564 a          253 c          299 g          691 t
ORIGIN      
        1 ctaatttttt taagtcaaca tgttctacaa gtgcttgaat gtctcctacc aatttttttg
       61 gtactttaaa atcttttcgc ggtcgtattt gctttgctaa tgtatgatga aaatgttgcg
      121 atttgataaa tgaaactatt aaactttttg atatttcggt aaatgaatta ctgatactta
      181 cggggccaat aaggtatctg tattcttcat ttttcagcaa aaagtacact atgcctctcc
      241 agagtaaaaa taatgaatga tgatgttttt gatattcagg tacaataaac gaacgtccca
      301 attcaagaga ttgagataga ataggtaaaa cagtgttttg aattttgaat aaactatgta
      361 tataaaagcc ttttttaccg tattgtttca atatttcatt gcctttacct attctatatg
      421 ctcctactac tttttttgca gttgtatccc atataaataa gtgttcatag tatatatcgt
      481 attcatctat gtcaatttcg ttgtttgtac cttcacctac tgctctaaaa gtaagttctc
      541 tgagtcttcc tatttctcgt attataaacg gaattcttac cgccgacgag caatatactt
      601 caaattgttt aaacgaaaat aatctatcgt ttttacgaat tgaatttatt tcttgttcaa
      661 gcataaccac cggaacaggt tctataatag gtttagggtc agtttgatta gtttgtttaa
      721 atatggtaaa aaaatctttt atttctatat ttttatacgc caatacatat acatgagctc
      781 ttacatagcg cgtaaggcta aaatctttat tgaatttttt ttgttcattg ctgctaattg
      841 ctgcaccaat tatacactgt aatgtgtaat tatttttttt aaaaaatggt aatcgtaatc
      901 gtttaagact taacacggga tacgtttttt tgcaatttcc aacattgtag catcggagtg
      961 ttgaatatat accggaatta ttggtatttc ggcgtatttt acagctccta atatatttgt
     1021 ttgccattct ccatctgttt tgtgttgttt gttaaattta taaatattgc cttgttgagc
     1081 aggaaaaaaa cctaatgctt gattgttttt gaagtgttca tacattttta taatacccga
     1141 tttatttggt gaacctttag aatcggcaaa aggatatgtt tggaaaaata ttttttcaaa
     1201 taccggattc tgaatggctc caatatcttc tacaatttta aaatcgggtc gtttttgtga
     1261 aataatctta taaagtaata atgcatctaa tcctcctaaa ggttgatttg ctatgacagc
     1321 aaaaggtcct gtagcaggaa tttgttcaag catatgttga ttatattgta tttcaatgtc
     1381 gagttgttta aaaaaggttt caatacagtg acttgcaaaa tagtcatcaa tagcatgtac
     1441 tgaagtgcct tgcagcattg tttcacaata ggaatgaaac atatgataca ttgattgata
     1501 tttgagtctg tttaattgtc cgatttttcg gatttgtgac tggctgagaa aagatgactg
     1561 aatcatattt tgtttttttt taacaaatat caatatcaaa aattattcaa atcataccgt
     1621 aatgttaatg aaatgtgaaa aatgtatttc gtttgttatt aaaatgttat taattagagg
     1681 taaggggggg ggagattagt ttgtgatatt tcacatgaag cgatatatga gtggagtttt
     1741 tagacattct gatatttggc acaattggaa aaagagagaa cgtaaacgca aaaacctaat
     1801 acgactt
//
LOCUS       DAOTOW010000508         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51106_length_1796_cov_3.036760, whole genome shotgun sequence.
ACCESSION   DAOTOW010000508 DAOTOW010000000
VERSION     DAOTOW010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51106_length_1796_cov_3.036760"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="P5243_08650"
     CDS             complement(<1..313)
                     /locus_tag="P5243_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRS19560.1"
                     /translation="MANLETSYLGLTLKNPLIVGSCGLTGNIESIKRILVHEPGAIVL
                     KSLFEEQINADIHKNILQNTEISAEAETYISSHIAHKNISDYCNLIQECKKITNIPII
                     AS"
     gene            complement(379..897)
                     /locus_tag="P5243_08655"
     CDS             complement(379..897)
                     /locus_tag="P5243_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRS19561.1"
                     /translation="METILLTYAPQGGYTEQAAYEIAQKITFAHCTILPGSKLTQKEL
                     ESYSRIIIGIATLGNDSWNSGRQDPDTVHLQKLITQVDFTNKKIALFGLGNAVLYPSH
                     FIDEVGIFEEHLQSKHATIIGYTPVSEYTFKDSKAIRGEYLCGLALDYDTEMHTAQSR
                     ISSWVTQLEKEL"
     gene            complement(900..1589)
                     /gene="pgl"
                     /locus_tag="P5243_08660"
     CDS             complement(900..1589)
                     /gene="pgl"
                     /locus_tag="P5243_08660"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRS19562.1"
                     /translation="MNIVYTHTISQIGDYLYESFQNALQKSPHITIALSGGNTPKALF
                     EYWVKAYKHSKIWHNISYYWVDERCVAPDNTESNYGVAFSLFFEPLTIPHQHIYRMQG
                     EQNPQKEALRYHALLQHINQNNGIPQFDFILLGIGDDGHTASIFPGNENLFFIPQFVV
                     NTKNPYTQQNRLTITGSVINNAKHVMFIVTGESKRNIVQEIIHPTRVTYPAHYVKPIN
                     GQLILLTDIQL"
     gene            complement(1589..>1796)
                     /locus_tag="P5243_08665"
     CDS             complement(1589..>1796)
                     /locus_tag="P5243_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRS19563.1"
                     /translation="FTRGDAVDLAWQFVQPILDEWENNPDFPLYGYPAGSWGPKEAND
                     LKVGYGDWRYPCKNLNSDGGYCEL"
BASE COUNT          540 a          273 c          320 g          663 t
ORIGIN      
        1 tactggcaat tataggaata tttgtgattt ttttgcactc ttgtataaga ttacaataat
       61 cggaaatatt tttgtgagca atgtgtgaac taatatatgt ttctgcttca gcactaattt
      121 cagtattttg aagaatgttt ttgtgtatat cagcgtttat ttgttcttca aataatgatt
      181 ttagtactat ggcacccggt tcatgtacaa gaatacgttt tatggattct atattgccgg
      241 taagtccgca gctgccaact ataagaggat ttttgagagt taatcccaaa tatgacgttt
      301 ctaaatttgc catacaaaaa tgtttttagt ataaggtatt tgatatataa caaatatact
      361 aaaaactttt tgatttattt acaattcttt ttctaactgt gtaacccatg atgaaattct
      421 actttgagca gtgtgcattt ctgtgtcata atctaaggca agtccacata aatattctcc
      481 tcttattgct ttagaatctt taaatgtata ttctgagaca ggtgtgtatc ctataattgt
      541 tgcatgtttt gattgtaaat gttcttcaaa aatacctact tcatcaataa aatgtgacgg
      601 gtataaaacg gcattaccca gtccaaataa tgcaattttt ttgtttgtga aatcaacttg
      661 tgttatcagt ttctgtaaat gaacagtatc aggatcttgg cgtccagagt tccatgaatc
      721 attgcctagg gttgcaatac ctattataat acgtgagtat gattctaatt ctttttgtgt
      781 tagtttactt cctggaagta tggtgcaatg tgcaaaagta attttttgtg ctatttcgta
      841 tgcagcttgt tccgtatatc ctccttgagg agcataggtt aataaaatgg tttccatagt
      901 tataattgta tatcggttaa taatataagt tgtccattta ttggttttac atagtgtgca
      961 ggatatgtaa cacgtgtagg gtgtataatt tcttgtacta tgtttctttt ggactctccg
     1021 gttacaataa acattacatg tttggcatta tttattacag atccggtaat agttaaacga
     1081 ttttgttggg tataaggatt tttggtattt acaacaaatt gtggtataaa gaaaagattt
     1141 tcgttcccgg gaaatataga tgctgtatgg ccatcgtctc ctatgcctaa tagaataaaa
     1201 tcaaattgag gaataccatt gttttgatta atgtgttgta aaagagcatg atatcgtaat
     1261 gcttcttttt gcggattctg ctcaccttgc attctatata tgtgttgatg aggaattgta
     1321 agtggttcaa aaaataatga gaaagcaaca ccatagttac tttcagtatt atcaggagcg
     1381 acacaccgtt cgtcaaccca atagtaggaa atattgtgcc atattttact gtgtttatat
     1441 gctttaaccc aatattcaaa aagagcttta ggggtatttc cgccagaaag agcaattgta
     1501 atgtgtggtg atttttgtaa agcattttgg aacgattcgt ataaataatc gcctatttga
     1561 gaaattgtat gtgtatatac tatattcatt ataattcaca ataaccgccg tcactattta
     1621 gatttttaca aggatatcgc caatctccat aaccaacttt taaatcgttg gcttctttag
     1681 gaccccaaga gcctgccggg taaccataca atggaaaatc ggggttgttt tcccattcat
     1741 ctaaaatagg ttgtacaaat tgccatgcta aatctactgc atcaccacgt gtaaag
//
LOCUS       DAOTOW010000509         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51174_length_1794_cov_3.879241, whole genome shotgun sequence.
ACCESSION   DAOTOW010000509 DAOTOW010000000
VERSION     DAOTOW010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51174_length_1794_cov_3.879241"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="P5243_08670"
     CDS             <1..592
                     /locus_tag="P5243_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19564.1"
                     /translation="SIPIHTPSPDLEIQQEQLIDISKSPPKKVRRIQYISRNTFYSHI
                     AKTESKGSYSIANRSLLLGKYQASKHALLEFGYSSDRIDQIYATVDTTYTTQGNPRYN
                     FDITLFPPEEQERFIRWYMQQMETKHLRKHIHKYSGKTIQNIHITKPGILYASMMGYA
                     HVQRFLESEGKYNYYAGSGKSIAGRLKKYANIEFSN"
     gene            complement(589..1068)
                     /gene="ribH"
                     /locus_tag="P5243_08675"
     CDS             complement(589..1068)
                     /gene="ribH"
                     /locus_tag="P5243_08675"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRS19565.1"
                     /translation="MATSLKNLSEYDYSSIPDASALSIGIVVAEWNIEINNALLQGAI
                     DTLLKHGVDKSNVFVNYVPGAFELTYGCKVMAENYDVDAIIAIGTVIQGDTPHFDYIC
                     QGVTYGISELNLSFDLPFIFGVLTVNTLEQAQERAGGKHGNKGVEAAVAAIKMALYD"
     gene            complement(1068..1751)
                     /locus_tag="P5243_08680"
     CDS             complement(1068..1751)
                     /locus_tag="P5243_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS19566.1"
                     /translation="MAQSKETQEIKQFEKIESTLGKAELYIEKNQKKLTYIVGAILLL
                     VAAFMAYRNFVVTPRESEAQNVIWQAQNAFAKDSFKIALYGNESVMGFSEIVDEYGST
                     KAGNLARAYAGFCCIRLGEYENAIDYLEDFEVNDPIVMPLAKCAIGDAYTELGKYDKA
                     VSNYLEAAKLSDHELTSPRFLMKAGLVYEKMNKKDKALDVYTQIKESYPTSNEATVID
                     KYITRVSLK"
BASE COUNT          605 a          333 c          234 g          622 t
ORIGIN      
        1 ttctattcct attcatacac cgtcacccga tttagaaatt caacaagaac aattaataga
       61 tatttcaaaa tcccccccca aaaaagttag gcggattcaa tatatttctc gtaatacctt
      121 ttactcacat attgcaaaaa cagaatcaaa aggttcttat tctattgcaa accgttcatt
      181 attactcggt aaatatcaag cgagcaaaca tgcattatta gaatttgggt atagttcaga
      241 tagaattgac caaatatatg ctaccgtaga tactacatac acaacacaag gtaatccccg
      301 ttacaatttt gatataacat tgtttcctcc cgaagaacaa gaacggttta ttcgttggta
      361 tatgcaacaa atggaaacaa agcatttacg gaaacatatt cataaatatt caggaaaaac
      421 tattcaaaat atacatatta ccaaacctgg tattttatat gcatcaatga tgggatatgc
      481 acatgttcag cgttttttgg aatcggaagg taaatataac tattacgccg gtagtggaaa
      541 aagtatagca ggaagattaa aaaaatatgc caatattgaa tttagcaatt aatcatacaa
      601 tgccatttta atagcggcta cagcagcctc tacacctttg ttgccgtgtt taccacctgc
      661 tctttcttga gcttgctcta atgtatttac tgttaaaaca ccaaatataa aaggtaaatc
      721 aaacgaaaga tttaattcag aaattccata ggtaacccct tgacatatat aatcaaaatg
      781 aggggtatct ccttggataa cagtaccaat agcaattata gcatctacat cataattttc
      841 tgccattact ttacaaccat aagtcaactc aaatgctccc ggcacataat ttacaaacac
      901 atttgattta tcaacaccat gttttaataa tgtatcaatg gctccttgta ataaagcatt
      961 gtttatttcg atattccatt cggcaactac aattccaatt gacaatgctg aagcatcagg
     1021 tattgaagag taatcgtatt cagatagatt tttaagtgat gtagccatta ttttaaacta
     1081 acacgagtta tatatttatc aataacagta gcttcatttg atgtaggata tgattcctta
     1141 atttgtgtat atacgtctaa tgctttatct tttttattca ttttttcata caccaatcct
     1201 gctttcatca aaaatcgagg acttgtaagt tcgtggtcac ttaattttgc tgcttccaaa
     1261 taatttgata ccgctttatc atattttcct aattcagtat aagcatcacc tatagcacat
     1321 ttggcaagag gcattacaat agggtcattt acctcaaaat cttcaagata atcaatagca
     1381 ttttcatatt ctcctaatcg aatacaacaa aatccggcat aagctctcgc taaattacct
     1441 gcttttgtac ttccatattc atctacaatt tcagaaaatc ccattaccga ttcattacca
     1501 tataatgcta ttttaaatga atcttttgca aatgcatttt gtgcttgcca tattacattt
     1561 tgtgcttctg actcacgagg agttacaaca aaatttctat atgccataaa agcagccact
     1621 aacagtaaaa ttgcaccaac tatatatgtt aatttttttt gatttttttc aatatataat
     1681 tctgcttttc caagtgttga ttcaattttt tcaaattgct ttatttcttg agtttctttt
     1741 gattgtgcca ttgttattat ttaataattg tattcctaat ttttcgtcaa caaa
//
LOCUS       DAOTOW010000510         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51322_length_1791_cov_2.499424, whole genome shotgun sequence.
ACCESSION   DAOTOW010000510 DAOTOW010000000
VERSION     DAOTOW010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51322_length_1791_cov_2.499424"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1791)
                     /locus_tag="P5243_08685"
     CDS             complement(<1..>1791)
                     /locus_tag="P5243_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRS19567.1"
                     /translation="VATVNGSQVQNNTGSQTQATISYVAGEPSQLIIQAGNNQTAMPG
                     TNVAIAPSVLVTDANGNPVSGVNVTFKVTQGNGEVSHTVVVTNASGIATLGYWELGPN
                     PGTNKVVATATSSSTSKAIRYPFPHATRQQYPYGISATNMDYTVFQNKLNTWISNYYV
                     EGNCYGTGDCARIKWDNKDLTVSEGIGYGMLIFVYTDNDANNYKDKFDKLWTYYKKWS
                     TSAGGLMSWRINGFTAVNGGDSGAATDADIDVALALIQAHKQWGSAGAVNYIQEAETI
                     LRKIYDSEVDGNKLFKPGDQWNSVQNPSYAALFAVKKAMEIQVAESFTQTRDWASVYT
                     ALQNYLAHYQNSTTGLWPNWTDPNATGSCVANPGWYSEGCWYGLDALRTPWRVAWDHA
                     WYGTANSASMLNKLSDWLDASYIGGDPTNVIGKFNNISTGTQTAYPLDAAGFVAGLAG
                     AYMANNNATDQENLNEWYANLMARDLSKPGNLSANDPLVLSDPATYGYQYYSPTLQIL
                     MGLTLSGNTPDFNTLEPVVAAPVEFIATGDGLLPISLLTFNATKKEQSVELEWKTASE
                     QNNDYFTLYRAVSNNNFTPIATVHGAGTSNV"
BASE COUNT          503 a          407 c          295 g          586 t
ORIGIN      
        1 cacattcgat gttcctgctc cgtgtactgt tgcaattggt gtaaaattgt tattcgatac
       61 cgcacgatac aatgtaaaat aatcattatt ctgctcactc gctgttttcc attccaattc
      121 taccgattgt tcttttttgg tagcattaaa cgttaacaat gaaattggca ataaaccatc
      181 acccgttgct ataaattcaa caggagctgc cactaccggt tctaaggtat taaaatcggg
      241 agtattacca cttaaagtta atcccataag tatttgtaac gtagggctgt aatattgata
      301 gccgtatgtt gccggatccg ataacactaa cggatcattt gcagataaat tacccggttt
      361 acttaaatct cgtgccatca aattggcata ccattcattc aaattttctt gatcggtagc
      421 attattattt gccatatatg cacctgcaag cccggcaaca aaacctgcag catcaagcgg
      481 ataggcagtt tgtgtacctg ttgatatatt attaaactta cctattacgt tagtggggtc
      541 acctccaata taacttgcat ctaaccaatc agagagttta tttaacatac ttgcactatt
      601 tgccgttcca taccatgcat gatcccatgc aacacgccac ggagtacgca atgcatcaag
      661 accgtaccaa caaccttcac tataccaccc cggattagca acacaagaac ctgtcgcatt
      721 cggatcagtc cagtttggcc atagtcctgt agtactattt tgataatgag ctaaataatt
      781 ttgcaaagcg gtatataccg atgcccagtc gcgtgtttgc gtaaaacttt cggctacttg
      841 tatttccata gcttttttta ctgcaaataa tgctgcataa ctcggattct gaactgaatt
      901 ccattggtca cccggtttaa ataatttatt accgtcaacc tcactatcat aaatttttcg
      961 caagatagtt tctgcttctt gaatataatt taccgcgccg gcacttcccc attgtttatg
     1021 ggcttgtata agtgccaatg ccacatcaat atcagcatcg gttgcagcac cggaatctcc
     1081 tccattcaca gcagtaaacc cgtttattct ccacgacatt aaacctcctg cacttgtaga
     1141 ccatttttta taatacgtcc acaatttatc gaatttatct ttataattat ttgcatcatt
     1201 atcggtatat acaaaaatta acataccata cccaatacct tcactcactg tcaaatcttt
     1261 attatcccat tttatacgag cacaatcacc ggtaccgtaa caatttcctt ctacataata
     1321 atttgaaatc catgtattta atttattttg aaatacggta taatccatat tagtagccga
     1381 aataccatag gggtattgtt gccgtgtagc atgggggaat ggatagcgaa tagctttact
     1441 tgtagaacta gaagttgcag tggcaaccac tttatttgtt cccggatttg gtcctaattc
     1501 ccaatacccg agagttgcaa ttccacttgc attggtaaca actacggtat gagacacttc
     1561 tccatttcct tgtgtaactt taaatgtaac atttacccca cttacaggat ttccgtttgc
     1621 atcggttact aacacacttg gtgctattgc cacatttgta cccggcattg ctgtttgatt
     1681 attacctgct tgaattatta attgcgatgg ttctccggct acatatgata tcgtagcttg
     1741 cgtttgcgaa ccggtattgt tttgaacttg actgccgtta accgttgcta c
//
LOCUS       DAOTOW010000511         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51633_length_1783_cov_3.857639, whole genome shotgun sequence.
ACCESSION   DAOTOW010000511 DAOTOW010000000
VERSION     DAOTOW010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51633_length_1783_cov_3.857639"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="P5243_08690"
     CDS             <1..516
                     /locus_tag="P5243_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19568.1"
                     /translation="ITLEMFTNNFFKNTKIYILFPLLIFSTYSFAQIRTGGNIGLNVY
                     NNILIIDLSPEIGYTFIDNALAGFSPFILYAKQLQTDEKQILYGARTFGEYKLEIGAF
                     AHVEYEISRLWTTEGFMKTMYALPVGGGFESPIGINTTAYFMLLYDVLYKPGISYRQN
                     PIIRAGLRYSF"
     gene            529..1656
                     /gene="holB"
                     /locus_tag="P5243_08695"
     CDS             529..1656
                     /gene="holB"
                     /locus_tag="P5243_08695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRS19569.1"
                     /translation="MLFSDIIGQNTIKQELLDTVKNNRISHALLFYGPEGSGKLSLAI
                     AYAQYINCENKQEFDSCGVCPSCLKYNKLQHPDLHFVFPVATNSKIKKDPVSDNFITE
                     WREIFLQQPYMSLDMWLAHIGVENKQGSIQKEESKEIMRKLNLKTYEAEYKVMIIWMA
                     EKMNETCANKLLKILEEPPAKTLFILISEHTEDLMATILSRTQIIRIPKIDDTSMRQK
                     LATDFNLQGEDLDTLVHNANGNYYKAFELISFENSNSDFFNYFTQIMRMAYAKNVPEL
                     YNIVESIAETGREKQKGFLEYSLKMLRENFILNHKKSEIVYLYKKEKEFSEKFHVFIN
                     ERNIAQLTKEFNDAHFHIERNGNAKIIFFDLFIKIMLLLRS"
BASE COUNT          691 a          263 c          264 g          565 t
ORIGIN      
        1 attactttag aaatgttcac aaacaatttt tttaaaaaca caaagattta catattattt
       61 cccttactta tatttagcac gtatagtttt gctcaaataa gaacgggagg aaatattgga
      121 ttaaatgtat ataataatat acttattatt gatctttcac ctgaaatagg ttatactttt
      181 attgataatg ctctagcagg tttttctcct tttatattat atgctaaaca attacaaacc
      241 gacgaaaaac aaattttata tggtgctcgc acatttggtg aatacaaatt agaaattggt
      301 gcatttgcac atgtagaata tgaaattagc agattatgga caactgaagg ttttatgaaa
      361 acaatgtatg ctttaccggt aggaggagga tttgaaagtc caattggaat taatactact
      421 gcatatttta tgcttttata tgacgtttta tacaaacccg gtatatcata cagacaaaat
      481 cccataataa gggcaggttt acggtattca ttttaaaata catagagtat gttattttct
      541 gatattattg gacaaaacac aataaaacaa gaactgttag acacagttaa aaacaataga
      601 ataagccatg ctctgttatt ttatggaccc gaaggttcgg gaaaactttc attggcaata
      661 gcctatgctc aatatataaa ctgcgaaaac aaacaagaat ttgactcatg cggagtttgt
      721 ccaagttgtt taaagtataa taaattacaa catcccgatt tacattttgt atttccggtt
      781 gccacaaatt ctaaaattaa aaaagacccg gtaagcgata attttattac agaatggcgt
      841 gaaatatttt tacaacagcc atacatgagt ctcgatatgt ggttagctca tataggtgta
      901 gaaaacaaac aaggaagtat tcaaaaagaa gaaagtaaag aaattatgcg taagcttaac
      961 ctcaagactt acgaagccga atacaaagta atgataattt ggatggcaga aaaaatgaat
     1021 gaaacatgcg ccaacaaatt acttaaaata ttagaagaac cgcctgcaaa aactttattt
     1081 atactcattt ctgaacatac cgaagattta atggcaacca ttttatctcg tactcaaatt
     1141 attcgcattc ccaaaattga tgatacaagc atgcgtcaaa aacttgctac cgattttaat
     1201 ttacaaggtg aagatttaga tacgttggtt cacaatgcaa acggaaatta ctataaagca
     1261 tttgaattaa tcagttttga aaattcaaat tccgattttt tcaactattt tacacaaatt
     1321 atgcgaatgg catatgccaa aaatgtaccc gaattataca atattgtaga atctattgct
     1381 gaaaccggaa gagaaaaaca aaaaggattt ttagaatatt cattaaaaat gcttcgcgaa
     1441 aattttattc ttaatcataa aaaatcagaa atagtatatc tgtataaaaa ggaaaaagaa
     1501 ttttcagaaa aatttcacgt atttattaat gaacgtaata ttgctcagct taccaaagaa
     1561 tttaacgatg ctcatttcca tattgaacga aacgggaatg caaaaatcat attctttgat
     1621 ttgtttatta aaataatgtt gctgcttcgt tcataaagtt tttttatttt tgcacatatt
     1681 tatacaaatt caacaatgga agataattca attcaacata tacctcacaa taaaccggtt
     1741 gcagtaacac cggaagaaat tgaaaaaaaa ataaaatcat cat
//
LOCUS       DAOTOW010000512         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51812_length_1779_cov_3.409513, whole genome shotgun sequence.
ACCESSION   DAOTOW010000512 DAOTOW010000000
VERSION     DAOTOW010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51812_length_1779_cov_3.409513"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="P5243_08700"
     CDS             complement(<1..331)
                     /locus_tag="P5243_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRS19570.1"
                     /translation="MNFIDYILVLPILWGGFRGFKNGLISEGGTVLALILGIWISVRF
                     SGSAGLYISQYFDVGEQYREVLAFTLLFLGVVILSFVCTRLLSHFFKTIHLQWLDILL
                     GIVFGATK"
     gene            complement(328..1551)
                     /locus_tag="P5243_08705"
     CDS             complement(328..1551)
                     /locus_tag="P5243_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRS19571.1"
                     /translation="MNISLYIARRYLLSKHNKNAINYITTVSVILVAFVTMALIIAMS
                     VFNGLTILITSMFSTFDPQIKVQSSYGRVFSADSAVQIIANTPNIAYYTPVLEEDVLF
                     VYANKQLVGKLKGVGEQYQKTCPVDSMIVSGEFVLQQNGIEYISVGQGVAHSLSVGLQ
                     FRDVMHMYAPDRNKKSSSINPMEDYNKEYAYARSVFSVQMDIDNMYVLSSLQLAQRLM
                     EYTHNEVSAIEIRCNEKADYKKVAAELQKKMGANYRVRDREQQHEFLYKITKSEKLIT
                     FLLVTFILIIASFSIAGALTMLIIDKKRDISILASMGAHRWLLQRVFIMEGWLISIVG
                     AVIGIIVGVSLTLLQQHLGLLKLYGESSSFIVEAYPVYLQLPDIVYVVSMVLLVGFLA
                     AFIPVRFIFKKHKIL"
     gene            complement(1558..>1779)
                     /locus_tag="P5243_08710"
     CDS             complement(1558..>1779)
                     /locus_tag="P5243_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19572.1"
                     /translation="SSVTSYYSYGFWGSISWKFSMKYPIQLLTISKLFFILLPFYYII
                     LFPWCLIFNFIDVYKHNSKGTGLIVHAYK"
BASE COUNT          677 a          319 c          211 g          572 t
ORIGIN      
        1 attttgttgc accaaatact attcccagca gtatatcgag ccattgcaaa tgtatagttt
       61 taaaaaaatg cgataataat cgagtacaca caaaagagag tatgactacg cctaaaaaaa
      121 gtaatgtaaa tgccaatact tctctgtact gctctcccac atcaaaatac tgcgatatat
      181 ataaaccggc agaaccggaa aaacgaacac taatccatat acccaaaatt aatgctaata
      241 cggttcctcc ttctgaaatt aagccatttt taaatcctct aaaaccgccc caaagtattg
      301 gtaatacaag tatataatca ataaaattca tagtatttta tgttttttaa atataaaccg
      361 aacaggaata aaggctgcta aaaaacctac cagtaaaacc atagaaacta catatactat
      421 atcaggcaat tgtaaatata caggatatgc ttcaacaata aaactacttg attcaccata
      481 taatttaagc agacccaaat gttgctgaag taaggttaaa cttactccta caataattcc
      541 tataacagct ccaacaattg aaataagcca tccttccata ataaaaacac gttgcaacaa
      601 ccaacgatga gctcccatgc ttgctaatat agaaatatca cgttttttat caattataag
      661 catagtaagt gctcctgcaa tactaaatga agctataatt agtataaatg ttaccaataa
      721 aaaagtaatt aatttttcac ttttagtaat tttatataaa aattcatgtt gttgttctct
      781 atctcttact ctataatttg cccccatttt tttttgtaat tcggcagcta ccttcttata
      841 atcagctttt tcattacatc ttatctcaat agcagatact tcgttatgag tatattccat
      901 taatcgttgt gctaattgta atgatgataa tacatacata ttatcaatat ccatttgtac
      961 agaaaacaca gatcgagcat atgcatactc tttattataa tcttccattg gattaattga
     1021 tgaacttttt ttatttctat cgggagcata catatgcata acatcgcgaa attgtaaacc
     1081 aacagacaaa ctatgtgcaa ctccttgccc tacagaaata tattctatac cattttgttg
     1141 taaaacaaat tcacctgaaa ctatcatact atcaacagga caggtttttt gatattgttc
     1201 tcctactcct tttaactttc caactaattg tttattagca taaacaaaca acacatcttc
     1261 ttccaaaacc ggagtataat atgcaatatt gggagtattt gctataatct gtacagcaga
     1321 atcagcagaa aaaactctac catatgatga ttgtactttg atttgaggat caaatgtact
     1381 aaacattgaa gtaataagta tggtaagacc attaaataca gacatggcaa taataagagc
     1441 catagtaaca aaagcaacta atataaccga tactgttgtt atataattta tagcattttt
     1501 gttatgttta cttaataaat accttcgtgc tatatacaac gaaatattca tactctatta
     1561 tttatatgcg tgaactatta atcctgttcc cttactatta tgtttatata catctataaa
     1621 attaaaaatc aaacaccatg gaaacagtat aatataatag aatggcaaca atataaaaaa
     1681 caattttgaa atagtaagta attgtatagg atacttcata gaaaatttcc aagaaatact
     1741 gccccaaaaa ccatatgaat aatatgatgt aacagaact
//
LOCUS       DAOTOW010000513         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51882_length_1777_cov_4.198026, whole genome shotgun sequence.
ACCESSION   DAOTOW010000513 DAOTOW010000000
VERSION     DAOTOW010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51882_length_1777_cov_4.198026"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5243_08715"
     CDS             complement(<1..285)
                     /locus_tag="P5243_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19573.1"
                     /translation="MIRIYTEPIFLPEKEYVFTVLFKEILGLDFSIHTSSVSRQYKIH
                     LPNNATIEFRDAFFSGFSESVGYCSTDNIPKKITYLKDVLPEQTIPVLYGD"
     gene            complement(386..778)
                     /locus_tag="P5243_08720"
     CDS             complement(386..778)
                     /locus_tag="P5243_08720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRS19574.1"
                     /translation="MATIFSKIVQGEIPAHKIAENADFLAFLDVNPLVMGHVLVIPKQ
                     EIDYIFDVDDNTLGNMMIFAKQIAIALKKTVPCKRIGVAVIGLEVPHCHIHLVPMQTM
                     ADINFMQPKLKPSQEELVAIKNAIIKNL"
     gene            complement(782..1255)
                     /gene="greA"
                     /locus_tag="P5243_08725"
     CDS             complement(782..1255)
                     /gene="greA"
                     /locus_tag="P5243_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRS19575.1"
                     /translation="MAKISYVTEEGLKKLQQELDYLTSVERPKISQQIAEARDKGDLS
                     ENAEYDAAKEAQGLLELRISKLQETIINSRIIDESKLDSSTVQILSKVKIKNTKNNAV
                     MEYTIVAESEADLKSGKISIDTPIAKGLIGKKVGDTVEIKVPAGVIPFEIIEISR"
     gene            complement(1382..>1777)
                     /locus_tag="P5243_08730"
     CDS             complement(1382..>1777)
                     /locus_tag="P5243_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19576.1"
                     /translation="VEIGSQLRVSDVKVTAYNKKGKPITRAKRVEKIEVAGTMLKNIL
                     AQPGKRTIFIRITAPGGVVLTQSSNNQFDYDGKTIMFSEKKEILYNNNDSRFEVYYNA
                     VNDNLEIGIYKIAIFCDGKEVGKSEIELK"
BASE COUNT          553 a          287 c          266 g          671 t
ORIGIN      
        1 atctccatat aaaacaggaa tagtttgttc cggtaataca tctttcaaat aggtaatttt
       61 ttttggaata ttgtcggtag aacaataacc taccgattcg gaaaatccgg aaaaaaatgc
      121 gtcgcgaaat tcaatagtag cattgtttgg taaatgaatt ttgtattgac gactaaccga
      181 cgaagtgtgt attgaaaaat caagtccaag tatttctttg aataatactg taaatacgta
      241 ttctttttct ggtaaaaaaa tgggttctgt ataaattcgt atcatagtgt atgaaaaata
      301 atgcctgtaa ctattttttt gtttaggatt atagtaaaaa tttacacaca aaaatacaga
      361 gttacaggca gtatttaatt aaaaattaga gattttttat aatagcattt tttattgcaa
      421 ctaactcttc ttgagacggt tttagttttg gttgcataaa gtttatgtct gccattgttt
      481 gcataggaac taaatgtata tgacaatgtg gtacttctaa acctattact gcaacaccaa
      541 tacgtttgca tggtacagtt tttttgagtg ctattgctat ttgtttggca aatatcatca
      601 tgtttcccaa tgtgttgtca tctacatcaa aaatatagtc tatttcttgc tttggaatta
      661 ctagcacatg ccccatcact aaagggttaa catcaagaaa tgctaaaaag tcggcgtttt
      721 ctgcaatttt atgtgcaggt atttcacctt gaacgatttt tgaaaagatt gttgccatat
      781 tctatctcga tatttcaatt atttcaaatg gaataacacc tgcaggaact tttatttcta
      841 ctgtatcgcc tacttttttt cctattaatc cttttgctat aggagtatca atagaaattt
      901 taccgctttt taggtcagct tccgattcag ctacaatggt gtattccatt acagcattgt
      961 ttttggtatt ttttatctta actttagaaa gaatttgtac cgtagaagaa tcgagttttg
     1021 attcatcaat aatacgtgaa ttaataatgg tttcttgtaa ttttgaaata cgtaattcca
     1081 ataacccttg agcctctttg gcagcatcgt attcagcgtt ttccgacaaa tcacctttat
     1141 cgcgagcttc tgctatttgt tgcgatattt ttgggcgttc tacacttgtt agataatcta
     1201 actcttgttg aagtttcttc aacccttctt ctgtaacata tgaaatcttt gccataggtt
     1261 aatgaatttt atataaaaca aaaggaatcc tacaagcatg cttataagat tcccaaaaat
     1321 tttgtattga ttcagcaaat atactacaaa aacaatctaa aaggaaatat tttgttgtaa
     1381 attattttaa ttcaatttct gattttccaa cttcttttcc atcacagaaa attgcaattt
     1441 tataaatacc aatttctaaa ttgtcattta cagcattgta atatacttca aaacgtgagt
     1501 cattattatt gtaaagtatt tcttttttct cactaaacat aattgtttta ccgtcataat
     1561 caaattggtt gtttgaagat tgagttaaca caacaccacc cggagctgta atgcgaataa
     1621 aaatagtgcg tttacccggt tgagccaaaa tatttttaag catggtaccg gcaacttcaa
     1681 ttttttctac acgttttgct cgtgttatag gtttgccttt tttattatat gcagttactt
     1741 ttacatcact tactcgcaat tgtgaaccaa tttcaac
//
LOCUS       DAOTOW010000514         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51890_length_1777_cov_2.932056, whole genome shotgun sequence.
ACCESSION   DAOTOW010000514 DAOTOW010000000
VERSION     DAOTOW010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51890_length_1777_cov_2.932056"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="P5243_08735"
     CDS             complement(<1..326)
                     /locus_tag="P5243_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRS19577.1"
                     /translation="MAKIGKIIGAAAGFALGGPIGALIGLAIGSVFDNTTVTVQSGTA
                     GYGQRVTPADFALSLMVLIAAVMKADGTVKRAELDFVKAYLRQAYGEQATLELLQVLR
                     DVLQKE"
     gene            complement(327..905)
                     /gene="coaE"
                     /locus_tag="P5243_08740"
     CDS             complement(327..905)
                     /gene="coaE"
                     /locus_tag="P5243_08740"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRS19578.1"
                     /translation="MKYIGLTGGIGSGKSVIAKIFECLQIPVYYSDIRAKQLMNTHED
                     IRNQLVDLVGEKVYSNNILQTHILAQALFSDVAIKNRVDSIVHPIVRNDFFEWAQLQC
                     APYVILESALMFQTNLYTYFSEIIFVDCNKQTRIQRVMARDGLQQHEVEKRIQMQPDD
                     EFCKQKSQYIIENNNTFVIPQVLSVHAKIINK"
     gene            complement(910..>1777)
                     /locus_tag="P5243_08745"
     CDS             complement(910..>1777)
                     /locus_tag="P5243_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19579.1"
                     /translation="NALNKTYTVDITLQAKYSHIPKTKAPITKAPEEIVVQVTASGFS
                     LLQYYMYSSQQIDVNIEKRLQDDIDGDSTLVINIAKEITDAHLFSKSIQIQSVKPEFV
                     TFIFAPVSKKKVKVVALYDIECLQQYQLSQTPVVIPDSIFVYGAQIVLESIDSVYTTK
                     IYARQVAESFTRKISLQPITNVTYSDTVVTVKASIEKFTEQQFTIPITPIHVPDSIII
                     DLMQHSVTITVFTGMSKTPQVSANDFKVIADFMKQNALTGEIPVEVVARSPWGRIVKV
                     SPEYVGYIIDTK"
BASE COUNT          553 a          318 c          261 g          645 t
ORIGIN      
        1 atttcttttt gcaaaacatc gcgtaatacc tgtaataatt caagtgtagc ttgttctcca
       61 tatgcctgtc gcaaatatgc ttttacaaaa tcaagttctg cacgttttac ggtaccatca
      121 gctttcataa cagcagctat aagcaccata agactcaaag caaaatcggc aggtgtaaca
      181 cgttgcccat accctgctgt accagattga actgttactg tagtattatc aaatactgaa
      241 cctattgcca aaccaattaa tgcacctata ggtcccccta aagcaaaccc cgcagcagca
      301 cctattattt ttccaatttt tgccatttat ttgtttataa tttttgcatg tacactcaat
      361 acctgaggaa tgacaaaagt attattattt tcaataatat actgtgattt ttgtttacaa
      421 aattcatcgt caggttgcat ttgtattcgt ttttccactt cgtgttgttg taaaccgtca
      481 cgagccatta ccctttgtat tcgtgtttgt ttattacaat ctacaaaaat aatttcagaa
      541 aaataagtat acaaattagt ttgaaacata agagccgatt ctaaaattac atagggagca
      601 cattgcaact gtgcccattc aaaaaaatca tttcgcacta taggatgtac tatagaatct
      661 accctatttt taattgcaac atcagaaaac aaggcttgcg caagaatgtg tgtctgcaat
      721 atattgttgc tatatacctt ttcaccaacc aaatcaacta attgattacg aatatcctca
      781 tgagtattca ttaattgttt agctcttata tcactataat atacaggtat ttgcaagcat
      841 tcaaatattt ttgctattac actttttcca cttcctatac caccggttaa tcctatatat
      901 ttcatggaat tattttgtat ctattatata acctacatat tcaggcgaaa cctttactat
      961 tcgtccccat ggtgaacgtg caactacttc tacgggaatt tctccggtta aagcattttg
     1021 tttcataaaa tccgcaataa ctttaaaatc atttgccgaa acttgtggtg ttttactcat
     1081 tccggtaaat actgtaattg ttacagaatg ttgcattaaa tcaataataa ttgaatcggg
     1141 aacatgtata ggagttattg gaattgtaaa ctgttgttcg gtaaattttt ctatcgatgc
     1201 ttttactgtt actaccgtat cactgtatgt aacatttgtt atgggttgta aagaaatttt
     1261 tctggtaaac gattctgcta cttgacgtgc ataaatttta gtggtataca cagaatctat
     1321 agattctaaa actatttgag cgccatatac aaatatagaa tcgggtatta caacaggtgt
     1381 ttgagataat tgatattgtt gtaaacattc tatatcatat aaagcaacta cttttacctt
     1441 ttttttactt accggagcaa atataaaagt aacaaattcg ggctttactg attgtatctg
     1501 aatactctta gaaaacaaat gtgcatcggt aatttctttt gcaatattta ttaccaatgt
     1561 tgaatcgcca tcgatgtcat cttgaagtct tttttctata ttaacatcta tttgttgcga
     1621 tgaatacatg taatattgca acaatgaaaa accacttgca gtaacttgta ccactatctc
     1681 ttctggtgct ttggtaatag gcgcttttgt tttgggaata tgagaatact ttgcttgtaa
     1741 tgttatatct acagtatatg ttttatttaa tgcattt
//
LOCUS       DAOTOW010000515         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_51971_length_1775_cov_3.356977, whole genome shotgun sequence.
ACCESSION   DAOTOW010000515 DAOTOW010000000
VERSION     DAOTOW010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51971_length_1775_cov_3.356977"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1427)
                     /locus_tag="P5243_08750"
     CDS             complement(150..1427)
                     /locus_tag="P5243_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRS19580.1"
                     /translation="MEILQKIYEMTAINNLIAEPQTLIMFAISFTLLYLGIVKKYEPL
                     LLVPISFGVLLANFPMGQLGIVPSQWVHQVFFSMDEASIRALSPQQLEEMAHNSPYMN
                     LFEIASKYGIMNYLYYALIKTGLLPPLIFMGVGAMTDFGPMLRNLRLSLFGGAAQIGI
                     FTVLFLAIAAGFTPKEAASLAIIGGADGPTAIFTTIKLAPHLLGPIAIAAYSYMALVP
                     VIIPLVVRLTCNEKELKINMKQQDKLYPSKHEIKNLRAVKIVFPIVLGTVIIIIVPSA
                     APLLGMLLFGNLVKEIGTDTQRLTVAATDFIMNPATIFLGLTVGATMTAEVFLKPQTL
                     MIIVGGFFAFAISVAGGIYAVKLTNLFSKKKINPLCGATGLSAVPMSSRVANEIALKY
                     DKSNHVLQYCMSSNVSGVIGSAVAAGVLIGFLQ"
     gene            complement(1487..>1775)
                     /locus_tag="P5243_08755"
     CDS             complement(1487..>1775)
                     /locus_tag="P5243_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRS19581.1"
                     /translation="VAPASSSAPAPKPVASSAAVSEVLSPIGGKFFKTKDSSETALKV
                     GDVVKEGQIIGYVEAMKVFNAISSTVSGTIVEIVYNSGDDVDEDDVLFKIQ"
BASE COUNT          637 a          341 c          258 g          539 t
ORIGIN      
        1 gataaatttt gttttattaa attaattttg tagttttatc ggacaataat gatttattat
       61 atattacaaa tatacacaaa ttatataaac aaaaaaagag gtttcaatta gttgaaacct
      121 ctttaagtac aatttattta tgttacaaat tattgcaaaa atcctattaa taccccggca
      181 gctacggcag acccaattac tcccgacacg ttacttgaca tacagtattg caaaacatga
      241 tttgatttat catacttcaa tgcaatttca tttgctacac gagatgacat aggaacagca
      301 cttaaaccgg tagcaccaca caatggattt attttctttt tactaaataa gttagttaat
      361 ttaacagcat atataccacc tgctacacta atagcaaaag caaaaaatcc acctacaata
      421 atcattaatg tttgtggttt caagaaaacc tcagcagtca tagtagcacc tactgttaaa
      481 cctaaaaata tagtagcagg attcataata aaatcagttg cagcaacggt taaacgttga
      541 gtatcggttc ctatttcttt tactaaattt ccgaacaaca acattcctaa taatggtgcc
      601 gcagaaggaa ctatgataat aataacagta cctaacacaa taggaaaaac tattttaaca
      661 gcacgtaaat tttttatctc atgttttgac ggatacaatt tatcttgctg tttcatattt
      721 attttcaact ctttttcgtt acatgttaat cgaacaacta acggaataat aaccggaaca
      781 agagccatat atgaatatgc tgcaatagca ataggtccca ataaatgcgg agcaagttta
      841 attgtagtaa aaattgctgt aggaccatcg gcaccaccaa ttatagcgag cgatgctgct
      901 tctttaggag taaatcctgc tgcaatagca agaaacaaaa cagtaaatat tccgatttga
      961 gctgcacctc caaacaatga taatcgaaga tttcgtaaca ttggaccaaa atcagtcata
     1021 gcacctaccc ccataaaaat aagcggcggc aataatcctg ttttaattaa cgcatagtac
     1081 aaatagttca ttataccata tttagacgct atttcaaata gattcatata cgggctgtta
     1141 tgagccattt cctctaactg ctgagggctc aatgctcgaa tagaagcttc gtccatagaa
     1201 aaaaacactt gatgtaccca ttgagaagga actataccta actgccccat agggaaatta
     1261 gcaagcaata ctccaaacga aattggaacc aacaacaatg gttcatattt cttaactata
     1321 cccaaataca acaatgtaaa agatatagca aacattatta atgtttgcgg ctcggcaatc
     1381 aaattattaa ttgccgtcat ttcatatatt ttttgtaata tttccatact aataattaaa
     1441 aaaagtaacc accgatattt aaatcggtgg ttacattata tatacattat tgaattttaa
     1501 acaatacatc atcttcatca acatcgtcgc cgctattgta aactatttca acaatagttc
     1561 cacttacagt tgacgatatt gcattgaaca ctttcatagc ttctacgtag ccaattattt
     1621 gaccttcttt tacaacatca cctactttaa gagccgtttc agatgaatct ttagttttga
     1681 agaatttacc acctataggg gataacactt cagaaaccgc agccgatgat gcaaccggtt
     1741 ttggagccgg agccgaagat gaggcaggtg caaca
//
LOCUS       DAOTOW010000516         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52250_length_1768_cov_3.621133, whole genome shotgun sequence.
ACCESSION   DAOTOW010000516 DAOTOW010000000
VERSION     DAOTOW010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52250_length_1768_cov_3.621133"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1768
                     /locus_tag="P5243_08760"
     CDS             <1..>1768
                     /locus_tag="P5243_08760"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRS19582.1"
                     /translation="LWDMFSYFGNAFHSKMLMYYPVRFISSQISEYTLYKFNIEFVLH
                     IILAGIGMYLLLLRFSCVSSIAFIIAVAYMLSGFFTGNAQHFWWVVSGAFLPFIILTY
                     YNLLQTPGFKQSLYFIFVFVCFVLGGYPAFTIVVGYALCIYFVYTIVFNAWNAHYMII
                     RAIIVQHIIIAIVCIILLLPVIVSYSDMIHAISRGNGVTLAQALWDSLHPKHCITFIF
                     PLLTAYTKNNFWHIDPSMMNMYIGLLPFIMALFSVLYSKKTHILFIWIVVLLSIAMSF
                     GTVLPLREFFYTYVPLFNVFRFPALFRLFTMFGLLVLAAYSFHYFKDYIHSKNFSYFL
                     IITAAITGIIALLYFVIPLVSHQIPYSQWYVLIELLNKKQLIGVQSLMLSVIAIFVFI
                     LHVLVFSKKGYSPYVGISLFVSLDMIVSVLLLSPVTIHNHIQITHIQHDLNKLPKQTA
                     IRNIFEQAYSQYSKELALPAPLWRNNQLLYKKRTYDGYTSFVYNKTNDFEKSVVYDSI
                     KTYPLLYIPQCIYTTNDTVFDFTKKIVILETQPILYKSIHTKLDSIEFVKSTARSVTC
                     KIQSSQEQYLVFMQNMYPGWKAY"
BASE COUNT          535 a          242 c          282 g          709 t
ORIGIN      
        1 gttgtgggat atgttttctt attttggtaa tgcttttcat agtaaaatgc taatgtatta
       61 tccggtacga tttatttcat cacaaatttc tgaatataca ttgtataaat tcaatattga
      121 atttgtattg catataattt tggcaggcat aggaatgtat ttgttgttgt tgaggttttc
      181 atgtgtatcg tctattgcat ttataatagc tgtagcgtat atgttgtccg gtttttttac
      241 gggaaatgca caacattttt ggtgggttgt aagtggtgca tttttgcctt tcattatact
      301 tacgtattat aatcttctcc aaactcccgg ttttaaacaa tctctgtatt ttatatttgt
      361 gtttgtttgt tttgtattag gtggttatcc ggcatttact attgttgtag ggtatgcttt
      421 atgtatttat tttgtgtaca caatagtgtt taatgcgtgg aatgctcatt atatgattat
      481 tcgtgctatt atagtacaac atatcataat agctatagta tgtattatat tgttgttgcc
      541 tgtaattgtt tcgtatagcg atatgataca cgctatttca cgtggcaatg gtgttacgct
      601 agcacaagca ttgtgggatt cattgcatcc aaaacattgt attacattta tttttccttt
      661 gcttacagca tataccaaaa ataatttttg gcatatagac ccatctatga tgaatatgta
      721 tataggatta cttcctttta ttatggcttt atttagtgta ttgtatagta aaaaaacaca
      781 tattttattt atatggattg ttgtattatt gagtattgca atgtcattcg ggacagtatt
      841 gccacttcgt gaattctttt atacgtatgt gccgttattt aatgtgtttc gcttccctgc
      901 attgtttcgt ttattcacta tgtttggttt attggtattg gcagcatatt cttttcatta
      961 tttcaaagat tatatacact caaaaaattt tagttatttt ttgattataa cagcagcaat
     1021 cacaggtatt attgcattac tttattttgt tatcccttta gtttctcatc aaataccata
     1081 tagtcaatgg tatgtattga ttgaattact taacaaaaaa caacttatag gagttcaatc
     1141 acttatgctt agtgtaattg caatttttgt atttatatta cacgttttag tgttttcaaa
     1201 aaaaggatat tctccatatg ttggaatttc tctatttgtt agtttagata tgattgtatc
     1261 ggtgttgttg ctatcgcctg ttacaattca taatcatatt cagattactc atattcagca
     1321 tgatttgaat aaacttccta aacaaacagc tatacgtaat atatttgagc aagcgtatag
     1381 tcaatattca aaagaattag cactgcccgc acctttgtgg agaaacaatc aattattgta
     1441 caaaaagcgc acatacgatg ggtatactag ttttgtatat aataaaacaa atgattttga
     1501 aaaatcggtt gtctatgatt caataaaaac atatccatta ttatatatac cgcaatgtat
     1561 atatactaca aatgataccg tatttgattt taccaaaaaa atagtaattc tcgaaacgca
     1621 accaattttg tataaatcta tacatacaaa acttgatagc attgaatttg taaaatcaac
     1681 agcacggagt gtaacatgta aaatacaaag ttcgcaagaa caatatttag tttttatgca
     1741 aaatatgtat ccgggttgga aggcatat
//
LOCUS       DAOTOW010000517         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52287_length_1767_cov_3.787383, whole genome shotgun sequence.
ACCESSION   DAOTOW010000517 DAOTOW010000000
VERSION     DAOTOW010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52287_length_1767_cov_3.787383"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="P5243_08765"
     CDS             <1..1522
                     /locus_tag="P5243_08765"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19583.1"
                     /translation="APRPYTELYYSGSQFQQQQVNVLHTQNYSRYLNAGVRLHYYKSI
                     GEYAPQTFAGKQVAPWIMYNGSRISAYSKYAMYSYDFDVNGGILDDSLLRKPHFIMSL
                     QNAHSKVIHHTASNLIKWNVLQKPVSIDTTNCNLIQYPLALGYTIHYDSYSHMYSHSN
                     LPKNWYAKTLWDTLNTKDTTLVSEIKNAVFIELNGNKTFANYNAIVSFGHQYNHTNMR
                     MYDASYPNEFANSLFVNTNLTGDFNPIALQVRGNYLHYMWGEYKGNNTLDIFISKDFE
                     IAPNNPLQVTFGFTNSNLKPQGIFNRYISNHYVWNNSFNQQHIQNLTFQTIFIKYKLQ
                     LDISKYYLQNYLYFNTKGMLLQSSESAQAFFAKVNKVSQIWKLTCSNGILLQTNSASG
                     IDYPQWATYNSIAFRNIVYKKLIDTYIGIEGLLYPKYFVPQYNTSLNVFTQQFDKEIG
                     GYPHVNAFFTIKYKPVRFMVTYNGLYTALYGKNFIALHYPQQSGYVTFAVSWLFYN"
     gene            1679..>1767
                     /locus_tag="P5243_08770"
     CDS             1679..>1767
                     /locus_tag="P5243_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRS19584.1"
                     /translation="MNQYETVFILTPVLSDIQVKESVEKYEQL"
BASE COUNT          647 a          236 c          252 g          632 t
ORIGIN      
        1 tgcacctcgg ccttataccg aattgtatta ttccggttct caatttcaac aacagcaagt
       61 aaatgtgtta catactcaaa attatagtag gtatttaaat gctggtgttc ggttacacta
      121 ttataaatca ataggtgaat atgcacctca aacttttgca ggaaaacaag tagctccttg
      181 gataatgtat aatggttcaa gaatttctgc atattcaaag tatgcaatgt attcgtatga
      241 ttttgatgta aatggtggaa ttttagatga ttcattatta cgtaaacctc attttattat
      301 gtcattacaa aatgcacatt caaaggtaat acatcatact gcttcaaatc ttataaaatg
      361 gaatgtgtta caaaaacctg tatcaattga tactactaac tgtaatctta ttcaatatcc
      421 tttagcatta ggatatacaa ttcattatga ttcttattca catatgtatt ctcatagtaa
      481 tttaccgaaa aattggtacg ctaaaacgtt atgggatacc ttaaatacaa aagatacaac
      541 acttgtgtct gaaataaaaa atgccgtttt tatagaactg aatggaaata aaacatttgc
      601 aaattataat gcaattgttt catttggaca tcaatataat catactaata tgagaatgta
      661 tgacgcttcg tatccaaatg aatttgcaaa ttctttattt gtaaatacta atctaacagg
      721 agattttaat ccgattgcct tacaagttag aggtaattat ttacactata tgtgggggga
      781 gtataaagga aataatacat tagatatatt tatatctaaa gattttgaaa ttgcaccaaa
      841 taatccttta caagtaacct ttggttttac aaattccaat ttaaaacctc aaggtatttt
      901 taaccgttat atatcaaatc attatgtgtg gaataatagt tttaatcaac aacacattca
      961 aaatttaacg tttcaaacta tatttataaa atacaagttg caattagata tatcaaagta
     1021 ttatttacaa aactatttgt attttaatac taaaggaatg ttgttgcaat catcggagtc
     1081 ggcacaagct ttttttgcaa aagttaataa agtatcacaa atatggaaat tgacttgttc
     1141 aaatggtatt ttgctacaaa caaattctgc atcaggaatt gattatcctc aatgggcaac
     1201 atataattca atagcgttta gaaatattgt gtacaaaaaa ttaattgata cttatatagg
     1261 aattgaaggt ttgttgtatc caaaatattt tgttccacaa tataatacat cattgaatgt
     1321 gtttacccag caatttgata aagaaatagg cgggtatcca catgtaaatg catttttcac
     1381 tataaaatat aagcctgttc gttttatggt aacctataat ggattatata cagcattata
     1441 tggaaagaat tttattgcat tgcattatcc tcaacaatca ggatatgtta catttgctgt
     1501 ttcgtggttg ttttataatt aatgaatttt aggtttgtta ttttttacat aaaatttata
     1561 gtatgttttg aatgaaaaaa cataaataaa tatagaaaac aatttgctac ataaaaaaat
     1621 atgtgtactt ttgccgctca aaaaattaat taaattattt aaatagtaag ctgttatgat
     1681 aaatcagtat gaaaccgttt tcattttaac tcccgtttta tctgacattc aggtaaagga
     1741 atcggtagag aagtatgagc agcttat
//
LOCUS       DAOTOW010000518         1766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52332_length_1766_cov_2.959088, whole genome shotgun sequence.
ACCESSION   DAOTOW010000518 DAOTOW010000000
VERSION     DAOTOW010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52332_length_1766_cov_2.959088"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="P5243_08775"
     CDS             complement(<1..174)
                     /locus_tag="P5243_08775"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRS19585.1"
                     /translation="MEKQKFHIEYEFNSSPKVLYNRLATPSGLAEWFADDVNFENGMQ
                     TYIFAWGKEQSKAH"
     gene            complement(396..1196)
                     /locus_tag="P5243_08780"
     CDS             complement(396..1196)
                     /locus_tag="P5243_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS19586.1"
                     /translation="MKIIPLVENVVYTHGLKAEHGLCIYIETDNTKILFDTGQSGLFL
                     ENAKRLHISIEDIDVLILSHGHYDHTGGLYEFIAHNTKAVIYAKKNICTPKFHADGRT
                     IGTPCKPEMLENRMTYLDSITHIDSQLSIIPNISIYSELDTHFSKMFVQSLDGLIEDT
                     FTDELFVVLHSSESISIITACSHRGITNICKTAHEIFKKPIYSVIGGFHTKDADSLQY
                     ELISQFCNTNAISYIGVCHCTGVEMFARMKQDIVAKVEYVYTGKYIEL"
     gene            complement(1193..1606)
                     /locus_tag="P5243_08785"
     CDS             complement(1193..1606)
                     /locus_tag="P5243_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS19587.1"
                     /translation="MWWIFALLSAFFAALTAILAKVGIKGINSDLATAIRTIFILIIA
                     WGIVFFRREISGLQGLTKTNIIFLALSGVATGLSWIFYFKALQIGNVSQVAPIDKLSV
                     VFALILSMIFLHEVITWKIAVGTACIVIGAIIIIL"
BASE COUNT          615 a          301 c          214 g          636 t
ORIGIN      
        1 atgagctttt gattgttctt ttccccacgc aaaaatatat gtttgcatgc cattctcaaa
       61 atttacatca tcagcaaacc attctgccaa ccccgatggt gtagccaatc tgttatagag
      121 aactttaggc gaagaattaa attcatattc tatgtgaaac ttctgctttt ccatttattg
      181 cgttttctga ttatttacaa cacataatat tacattatat gttatttata ttcaaataaa
      241 tatatttata ttactacctt acgaaacaaa tcgcaagata ctaataaaat ttgaatcaag
      301 aaaataattt aattcgaatt aattattgga aatttttatt gtatatgctt taataaaact
      361 tattacaaat atttgttatc tctccataat aagccttata attcaatata ctttcccgta
      421 tacacatatt ctacctttgc aacaatatct tgcttcattc tagcaaacat ttctacacct
      481 gtacaatgac atactcctat atatgatata gcatttgtat tacaaaattg agaaattaac
      541 tcatattgca aactatctgc atctttagtg tgaaaccctc ctataactga ataaataggt
      601 tttttaaaaa tttcatgtgc tgttttacat atattggtaa tacctctatg agaacaagcc
      661 gtaattatag aaattgattc agaagaatga agaacaacaa aaagttcatc ggtaaatgta
      721 tcttctatta atccatctaa agattgtaca aacatttttg aaaaatgtgt atccaattca
      781 gaataaatag atatattggg aattattgat aattgtgaat caatatgtgt aatggaatct
      841 aaatacgtca ttctattttc taacatttca ggtttacagg gtgttcctat tgtacgtcca
      901 tcagcatgaa atttcggtgt acatatattt tttttagcat aaatgactgc ttttgtatta
      961 tgggcaataa attcatacaa tcctccggta tgatcataat gcccgtgaga caatatgaga
     1021 acatctatat cttcaattga aatatgcaat ctttttgcat tttccaaaaa aagacctgac
     1081 tgacctgtat caaaaagtat ttttgtatta tcagtttcaa tatatataca taacccatgt
     1141 tcggctttaa gcccatgggt atacacaaca ttttctacta atggaattat tttcataaaa
     1201 taataattat agcccctatt actatacaag cagttcctac agctattttc cacgtaataa
     1261 cttcatgtaa aaaaatcatt gataatataa gagcaaatac tacactcaat ttatcaatag
     1321 gtgcaacttg tgatacattt cctatttgaa gagctttaaa ataaaatatc cacgacaaac
     1381 ctgttgctac gcctgataat gctaaaaaaa taatatttgt tttagttaac ccctgcaatc
     1441 cggagatttc acgacgaaaa aaaactattc cccatgctat tataagtata aatattgttc
     1501 taatagcagt agctaaatca gaattaatac ctttaatacc tactttagcc agtattgcag
     1561 taagtgctgc aaaaaatgct gataataatg caaatatcca ccacattggt tttttatgta
     1621 ttatttattt gtaacatttc ccatacttga gcggtaacat cggctaccat tgtagtacaa
     1681 ataatagcat gatgttttct ctgtttcaca tcttctttac tcacatattt tttcaacaat
     1741 tcaccacatt gtgttgttgc atgttt
//
LOCUS       DAOTOW010000519         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52371_length_1765_cov_3.346784, whole genome shotgun sequence.
ACCESSION   DAOTOW010000519 DAOTOW010000000
VERSION     DAOTOW010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52371_length_1765_cov_3.346784"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="P5243_08790"
     CDS             <1..997
                     /locus_tag="P5243_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19588.1"
                     /translation="NDWWVFDIIENTWTLLNSNVSSTCASQKTAIKIHNKVYTSGGNS
                     SNGTTLSDFGVFDLNNISSGYTAKTSMIEPLAGSAGFKKDGNVYLYGGKNNDWDAATP
                     GKQPSYSSNTWKYDVINNFWSMIATNQTKSENESQSDIIELTEMAYTQDTLENFFYVF
                     GGKTYNYETNTEIYSNEIYKLDLNTFSWTKYTTTLPYGLADFTASYIHGTNADTETII
                     LLGGQKASGTISTNLFKFIPTTGELITIPLTTSIKEYNLNSKISLYPNPAKDLLYIKH
                     ADKNTIVTIYNIAGKEIITTKPHNEIININKLAKGVYTIRIQSENTEITDTFIKQ"
     gene            1344..>1765
                     /locus_tag="P5243_08795"
     CDS             1344..>1765
                     /locus_tag="P5243_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19589.1"
                     /translation="MSIRNISLYVFSCVMLFACTQEQEKESTQIQEFQSKSMVGIIDG
                     KIFSVPSPYEFIHFINELQITYNHTLINPVNNIKLYESNLKKCLNFGIYGVDVAYVSV
                     FNQTSTALSYFSVLKQLSENIGLSQVFDAQTIERLENN"
BASE COUNT          701 a          273 c          244 g          547 t
ORIGIN      
        1 gaatgattgg tgggtatttg atattattga aaatacttgg actcttctaa attcaaacgt
       61 ttcttctact tgtgcttctc aaaaaactgc aattaaaata cataataagg tttacacttc
      121 aggagggaac agctcaaatg gcaccacgtt atcagatttt ggagtatttg atttgaataa
      181 tatttcgagc ggatataccg ctaaaacttc aatgatagag ccattagcag gaagtgccgg
      241 ttttaaaaaa gatgggaatg tgtacttgta tggtggcaaa aacaatgact gggatgcagc
      301 tacccccggt aaacagccta gttatagttc aaatacatgg aaatacgatg taataaataa
      361 cttttggagc atgattgcta caaaccaaac aaaaagcgaa aacgaaagcc aaagtgatat
      421 aatagaatta acagaaatgg cttatactca agatactttg gaaaatttct tttatgtttt
      481 tggtggtaaa acgtataatt acgaaacaaa taccgagata tacagcaatg aaatatacaa
      541 acttgattta aacacatttt cgtggactaa atatacaacc actcttccgt atggtttagc
      601 tgattttacc gcatcataca tacacggcac aaatgctgat acagaaacaa taattttact
      661 tggaggacaa aaggcttccg gcactatatc aacaaattta ttcaaattta ttccaactac
      721 aggtgaatta attacaattc cacttaccac atcaattaaa gaatataatc tcaactcaaa
      781 aatttcattg tatcccaatc cggctaaaga cttgttatat attaaacatg ccgataaaaa
      841 cacaatagta acaatataca atatagcagg caaagaaata attacaacca aaccacacaa
      901 cgaaattata aatattaata agcttgctaa aggggtgtat acaattagaa tccaatcaga
      961 gaatacagaa attacagata cgtttataaa acaataacac acatatcagt gtgcttgtgc
     1021 ataaaaaagc ccaagcacac caaatgtaaa actaccaaaa aactaccttc aaaaaacaga
     1081 tagaaaagaa aataaaatct catactgttt atatttctga ctcttattac atttaaataa
     1141 acgattataa atcaattgtt ttgtattgta aaaaaaagaa caaacacatc ttttattatt
     1201 actttttaac attttgtatt gttttaaatc tttttttgta tttttataga gttaaatact
     1261 tgtatacaaa atgtatgata tataaatata ttgtattata gtatttttaa catataggga
     1321 gttagaatag gaaaaaataa agtatgagta taagaaatat ttcattatat gtttttagtt
     1381 gtgtaatgct ttttgcatgt acccaagaac aagaaaaaga atcaactcaa atccaagaat
     1441 ttcaatcaaa atcaatggtt ggtattattg acggcaaaat atttagtgta ccctccccgt
     1501 atgaatttat tcatttcata aacgaattac aaatcacata caatcataca cttataaatc
     1561 cggtaaacaa cataaaactc tatgaatcaa acttaaaaaa atgcctgaat tttggaattt
     1621 atggtgttga cgttgcatat gtttctgttt ttaatcaaac atcaactgcc ctatcatatt
     1681 tttctgtttt aaaacaatta tcagaaaata ttggattatc gcaagtattt gacgcccaaa
     1741 caatagaacg cttagaaaat aatat
//
LOCUS       DAOTOW010000520         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52442_length_1763_cov_3.812061, whole genome shotgun sequence.
ACCESSION   DAOTOW010000520 DAOTOW010000000
VERSION     DAOTOW010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52442_length_1763_cov_3.812061"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="P5243_08800"
     CDS             complement(<1..391)
                     /locus_tag="P5243_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19590.1"
                     /translation="MKQGNVQKHLNRQRGLITRVALLIAMVSLFVTAGFAQRNYPFPQ
                     NYKYPYGQIYTGTDVQTKIQSLYNAWKTTYYVEGTMTGGPQSGKAAARIKFLQPGQDG
                     SLTVSEGIAYGMLITVYMDNATNNTQSM"
     gene            complement(676..1419)
                     /locus_tag="P5243_08805"
     CDS             complement(676..1419)
                     /locus_tag="P5243_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRS19591.1"
                     /translation="MRTFLRNSLYSILGLSAYLRLISFLYITAIRCGLWKSKYSELYF
                     LRNIVKPGDTCIDIGANLGYYSTQLASIVGSSGRVYAVEPIPLFGQIWKKNCKPHVNK
                     QLILFPYALGAQTDVVRMGIPKYHGRVHHGMTKIANTSHEEYVEFFDVEMRNPDELFS
                     GIPNIQFIKCDVEGYESVVFTNCIQTLQKHKPIIQTELSGTQNRIEVIQLLESLGYTM
                     YVLRSHALVEVSLSEALELSQDFYFIHNK"
     gene            complement(1419..1574)
                     /locus_tag="P5243_08810"
     CDS             complement(1419..1574)
                     /locus_tag="P5243_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19592.1"
                     /translation="MIELLLKAFGIVVITMVLVRLISRYILPWFVKTFFPNKYESFKK
                     EYLEPNE"
BASE COUNT          601 a          303 c          240 g          619 t
ORIGIN      
        1 acatactttg cgtattattg gtagcattgt ccatatatac ggttataagc ataccatacg
       61 caataccttc agacactgta agtgaaccgt cttgccccgg ttgaaggaat ttaatacgag
      121 cagcagcttt accgctttgt ggacctccgg tcatagttcc ttctacataa tatgtagttt
      181 tccatgcatt gtacaaactt tgaatttttg tttgcacatc ggttcccgta taaatttgac
      241 catacggata cttgtaattt tgtggaaacg gataatttcg ctgtgcaaaa cctgccgtta
      301 caaacaacga taccattgct atgagcaatg ctactcgtgt aattaaaccg cgttggcgat
      361 ttagatgctt ttgtacattt ccctgtttca tacctataga tttaattcaa aaattattta
      421 caaaatattt attttttata ttacatttct caacaagttc acaaaaatac acattttttt
      481 ggaaactaaa taccgaaaca atgcattttt tacacacact cttcgctatt gtatatcaaa
      541 catttacatc aaaacatgta ttgattgtat ttcattcttt taagaaaaga caaaaaatat
      601 gtatttatcc ctatattaaa attatgtata gcttaaaagt tttgaataaa agaaattcaa
      661 aaaaataatt tcttattatt tgttgtgaat aaaataaaaa tcttgtgaaa gttctaatgc
      721 ctccgataat gacacttcta ctaatgcatg agagcgaaga acatacatag tatatcctaa
      781 cgattctaat aattgaatta cttctatacg attttgagta ccgcttaact ctgtttgtat
      841 aattggtttg tgtttttgca atgtttgtat acaattagta aacacaacag attcatatcc
      901 ttctacatca cattttataa attgtatgtt tggtatacca gaaaataatt catcggggtt
      961 tcgcatttca acatcaaaaa attcaacgta ttcttcatga gaagtattag caatcttagt
     1021 cattccatga tgtacacgac catgatattt tggaataccc atacgtacta catcagtttg
     1081 agctcccaac gcataaggga acaatattaa ttgcttattt acatgtggct tacagttttt
     1141 tttccaaatt tgaccaaaca acggtatagg ctctacagca taaactctac cacttgagcc
     1201 gactattgat gctaattgtg tagaataata tcctaaatta gctcctatat ctatacacgt
     1261 atcaccgggt ttaacaatgt ttcgtaaaaa atacaattca gaatatttcg atttccataa
     1321 tccacaacga attgcggtaa tatataaaaa actaataagt cttagatacg cagataatcc
     1381 caaaatggaa tataaactat ttcggagaaa tgttcgcatt attcgttggg ttctaaatat
     1441 tcctttttaa aagattcata tttatttgga aaaaaggttt taacaaacca tggaagtata
     1501 tatcgagaaa taagtcttac taaaaccata gttataacaa ctattccaaa ggcttttaat
     1561 aataattcta tcatgtaacg aatgtttttt tatgaataca aaaattcacc gtattcactt
     1621 tgtattgtta attgtgttgt ttcagaagct tctactctac ctatgatttg tgcatcaatt
     1681 ccaaaagaat gtgaaatatc aataattttt tgagcaaatt gctgatttac atataattcc
     1741 atacgatgcc ccatgttaaa cac
//
LOCUS       DAOTOW010000521         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52454_length_1763_cov_2.765222, whole genome shotgun sequence.
ACCESSION   DAOTOW010000521 DAOTOW010000000
VERSION     DAOTOW010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52454_length_1763_cov_2.765222"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..888
                     /locus_tag="P5243_08815"
     CDS             196..888
                     /locus_tag="P5243_08815"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HRS19593.1"
                     /translation="MKKKENQLVSLIFNIVIPVLIMTKLSGTEGRFALGPAYSLIIAV
                     SFPLIYGLYHFVQAKKVNFISVLGFASVLLTGVMGLLTFIDPFWFAIKEAAVPAIIAI
                     VVLVSIYYNHNIIQKILFNDEIMDMERVNELLKQNEKVDLFKKTITQASYWVVVSFIV
                     SSILNFTLARVILTAQPGTTEFTEQLGTMNGLSFPVIAVPCTIILVVVMFFVFKKIKE
                     YTGLQLEDIVKK"
     gene            1021..>1763
                     /locus_tag="P5243_08820"
     CDS             1021..>1763
                     /locus_tag="P5243_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19594.1"
                     /translation="MKQLFISIVLVFIGNITTVNAQQDTVKSVCASQKTEIQTPSDFD
                     IWMRSDWGFSMTGGTNYFLGDISSHDVVVLKPNFGIGLEKKLNEYILWKGNFMQGRLS
                     GEKEKFEMGGIANLYFIAKITEYSSVFEFDLLDVLQGPQERKASLNAYAGFGIVDFRT
                     QRKNLVTKQVLQSYGFDGFQLAKPTREMVYPVGVVFEYNVTKALAFNFDVSLRIVNSD
                     KVDAYIANANNTIFADMYNYTGIGFTYKFG"
BASE COUNT          620 a          206 c          303 g          634 t
ORIGIN      
        1 ggtgtaacag aagttgattt ttctgaagag gttactttaa aagtaaaagt ttctaatgat
       61 atttttgtgg attataccgt aaaggttgta tctccgtaat tacatgtaaa aacattataa
      121 aaaaaggctg tctcatttgg gcagcctttt ttatttacat atttttgtat tgtaaataat
      181 agaaaatagc aaagaatgaa aaaaaaagaa aatcaattgg taagtcttat ttttaatata
      241 gtaatccctg tattaattat gactaaatta agtggaactg aaggtcgttt tgcgttagga
      301 cctgcgtata gtttaattat tgcagtatca tttcctctta tatatggttt gtatcatttt
      361 gtacaagcaa aaaaagtaaa ctttatttct gttttaggat ttgccagcgt cttattaacc
      421 ggtgtcatgg ggttattaac atttattgat ccattttggt ttgcaattaa agaagctgca
      481 gtacctgcta taattgcaat agttgttttg gtttcaatat attataatca taatattata
      541 caaaaaattt tatttaacga tgagattatg gatatggaac gtgtaaatga gcttttaaaa
      601 caaaatgaga aagtagactt atttaaaaaa acgattactc aagcatcata ttgggttgtt
      661 gtttctttta tagtttcatc aattttaaat tttactttgg ctcgggtaat tttaacagct
      721 caacccggaa caaccgaatt tactgagcaa ttaggaacaa tgaatggatt aagttttccg
      781 gtaattgctg taccttgtac tataattttg gttgttgtaa tgttttttgt attcaaaaaa
      841 attaaagaat ataccggact tcaattggag gatattgtaa agaaataaag gtatgagcat
      901 ctaatatata gatttaaaat tgaatattct ttcataattt gttgatattt agttgtacaa
      961 cttttttatc tttataaact tattaactac atttgataaa ctttaaaaac acaaaaagct
     1021 atgaaacaat tatttataag tattgtatta gtctttatcg gtaatataac aacagtcaat
     1081 gcacaacaag atactgtaaa aagtgtatgt gcttcacaaa aaacagaaat acaaacacca
     1141 tcagatttcg atatttggat gcgttctgat tgggggttct ctatgacagg tggaacaaat
     1201 tattttttag gagatatttc ttcacatgat gttgttgttt taaaaccaaa ttttggtatt
     1261 ggacttgaaa aaaaattaaa tgaatatata ttgtggaaag gaaactttat gcaaggacga
     1321 ttatccggag aaaaagaaaa atttgagatg ggcggtattg caaacttgta ttttatagct
     1381 aaaattacag aatacagttc agtttttgaa tttgatctat tagatgtttt gcaaggacca
     1441 caagagcgta aagcatcact aaatgcatat gcaggatttg gaatagttga ttttagaaca
     1501 caacgaaaaa atcttgtaac aaagcaagtt ttacaatcat atggatttga tggctttcaa
     1561 ttggcaaaac ctaccagaga aatggtgtac ccggtgggag ttgtatttga atataatgta
     1621 actaaagcac ttgcttttaa ttttgatgta tcacttcgaa tagttaattc ggataaggtt
     1681 gatgcctata ttgcaaatgc caataataca atttttgccg atatgtataa ttacacagga
     1741 attggtttta cgtataaatt tgg
//
LOCUS       DAOTOW010000522         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52538_length_1761_cov_3.424385, whole genome shotgun sequence.
ACCESSION   DAOTOW010000522 DAOTOW010000000
VERSION     DAOTOW010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52538_length_1761_cov_3.424385"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1761)
                     /locus_tag="P5243_08825"
     CDS             complement(<1..>1761)
                     /locus_tag="P5243_08825"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRS19595.1"
                     /translation="PASVGTAIATGKKSAYKAVGVGIDSSKLETILERAFLVGKATGI
                     VTNLPLSKSAAASFVAHYPTCDSSRAVFNQLLGSHLSIAIGAGNPFYNNNGQLENTPN
                     YTYVSQSDWNALTQGLTQFDNSTQVTDIDNDNIPDAWTLVQSESDILTSNAKRIIAMP
                     QVFDAIQFKRSSSSFLNNVPHLAELTQKGISHLAQKPQGFVLVVESGSVDYASQLQNN
                     EAILANMQELHTTIDAIQNWVSTESSWNQTLVIIVGTYETGFLSGMEFSSTAAAPDFY
                     SKTIHITQGAQGTIPLMKCNSNKATNMLTPLYANGIGSELFSHYTDEEDYVYGKYINN
                     SEIGQICMRLLPTPTSIPKTPKNIILMINDGVGLNQIRAANYYTGKTQQYEQFPVRLF
                     HSTYPLVTEENIRLASYNNSYESTLAWTDGVYLRNRTNATCSGASGTAIASGTKTYYY
                     GLGVDVHKNAVNTIARHAKSLGKSIGVATNIPISDATPAVFFANNVSRNNAAEITRQL
                     IIESKADVIIGGGHPEYDKHGQLLSKPNYKDIGGLEFWQDIRAGVTTFRTPSNSGWTQ
                     VQDIDGDNVPDAWTLIEDST"
BASE COUNT          558 a          332 c          290 g          581 t
ORIGIN      
        1 cagtgctatc ttcaattaaa gtccatgcat caggcacatt atctccatct atatcctgta
       61 cttgagtcca gcccgaatta cttggtgttc gaaatgttgt aactccggca cgtatatcct
      121 gccaaaattc taaaccacca atatctttat aatttggttt tgatagtaat tgaccgtgtt
      181 tatcatattc cggatgccct cctcctatta taacatctgc ctttgattct attataagtt
      241 gtcttgttat ttcggcagca ttattccttg aaacattatt agcaaaaaac actgccggag
      301 tagcatctga aataggtata ttagtagcaa ctcctattga tttacccaaa gatttagcat
      361 gacgagcaat tgtatttacc gcatttttat gaacatctac tcctaatcca taatagtacg
      421 tttttgttcc tgaagcaatt gcggttcccg aagctcctga acatgtagca tttgtacgat
      481 ttcttaaata aactccatcg gtccaggcaa gtgtagattc atatgaatta ttatacgatg
      541 ccaaacgaat attttcttcg gtcactaatg gataagtact atgaaacaat cgaacaggaa
      601 attgttcata ttgttgtgtt ttacctgtat aataatttgc tgcacgtatt tgatttaatc
      661 ctacaccatc atttatcata agaataatat ttttgggagt tttgggtata gatgtaggag
      721 ttggcaataa tcgcatacat atttgaccta tttcggaatt atttatatat tttccgtaaa
      781 catagtcttc ctcgtcagta taatgagaaa acaattcact accaatacca tttgcatata
      841 atggtgtaag catatttgta gctttattac tattacactt catcaaagga atagttccct
      901 gagccccctg agttatatgt attgttttag aataaaaatc aggagcagcg gcagtagaag
      961 aaaactccat acccgacaaa aaaccggttt cgtatgttcc aactataatt acaagcgttt
     1021 gattccaaga actttctgta ctaacccaat tctgtatagc atcaatagtt gtatgcaatt
     1081 cttgcatatt tgctaatatg gcttcattat tttgcagttg cgatgcgtag tcaacagaac
     1141 cggactcaac aactaacaca aacccttgtg gtttttgtgc caaatgagat ataccttttt
     1201 gagttaactc agctaaatga ggaacattgt ttaaaaaaga tgatgatgac cgtttaaatt
     1261 gaattgcatc aaatacctgt ggcatagcaa taattcgttt agcattagaa gttaaaatat
     1321 cagattcaga ctgcaccaac gtccatgcat caggaatatt atcattgtca atatctgtta
     1381 cttgagtact attatcaaat tgagtcaaac cttgtgtaag agcattccaa tcagattgtg
     1441 aaacataggt ataattaggt gtattttcca attgtccatt attattatag aatggattcc
     1501 cggctccaat tgcaatagaa agatgcgaac ccaacaattg attaaaaaca gcacgagaac
     1561 tatcacacgt aggatagtgt gcaacaaatg aagctgcggc tgattttgat aaaggtaaat
     1621 ttgtaactat accggtagcc ttaccaacta aaaaagcacg ttctaaaatt gtttcaagtt
     1681 tagagctatc aataccaacc cctaccgctt tataagccga ttttttacct gttgctattg
     1741 ctgttccaac agaagcaggg t
//
LOCUS       DAOTOW010000523         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52545_length_1761_cov_2.807151, whole genome shotgun sequence.
ACCESSION   DAOTOW010000523 DAOTOW010000000
VERSION     DAOTOW010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52545_length_1761_cov_2.807151"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="P5243_08830"
     CDS             complement(<1..197)
                     /locus_tag="P5243_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19596.1"
                     /translation="MKKLILGIAMLCIAFLSQAQTMLENVIVEKFNTDANGRVTYRIF
                     IDMAPGAQLSTIFGDPDHTLR"
     gene            complement(253..>1761)
                     /locus_tag="P5243_08835"
     CDS             complement(253..>1761)
                     /locus_tag="P5243_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19597.1"
                     /translation="KSLTIQPGTLIKGRAHGGVAENAAALIVSRGGKIFASGTETCPI
                     VFTAEADNLDGTYPIGNVGKWGGIALLGQAQNCINGGENGNCIAAGLGVVEGFDVLNP
                     YNWYGADINNSKGNGAEQFNDDDNSGIMRYVSIRHAGCEIAQGNELNGLSLASVGRGT
                     TIENIEVISNDDDDIEIFGGTVNLRYITSMFGCDDMLDYDLGWNGTAQFIFGIHEPNN
                     TNGNLWPGSSDNGLEGDGDDKNLTTTFDKTKRSNPTIYNATLVSNGNFTPTADNTGVA
                     GIMAKDRTQGSIYNSVFVNFRSGLHLATNATEGNAYQNWSNTATELTLSIPNSLKVKN
                     NTFIGCDMPMSIGALVSGKNATIQSVTPTAPSAADLTQFTTTDKNIVIPAGSLDPKFG
                     FAYVWAMNTATNAVTTKYDVVPSSIAATVSGTANPFYSNEAAPQAKGMIPVAYRGAFA
                     PTGKSWVSGWTLAKLLQTTAGLQNNPTDINQDGKTDVTDFNMLASKFGQANL"
BASE COUNT          531 a          339 c          296 g          595 t
ORIGIN      
        1 aaacgtagtg tgtgatcagg atcaccgaat attgtactta attgagctcc gggagccata
       61 tcaataaata ttcgatacgt tactctacca tttgcatcgg tattaaattt ttcgactatt
      121 acattctcta acatagtctg tgcttgagaa aggaatgcta tgcacagcat agctattcct
      181 aaaattaatt ttttcattgg tttaaaagtt ttatggttgt tttattttat ttttttatgg
      241 ttgtttgttg ttttatagat tagcttgacc gaatttagat gctaacatat tgaagtcagt
      301 tacgtcagtt ttcccatctt ggttaatgtc agttgggttg ttttgtaaac ctgcagttgt
      361 ttgtaacaat ttagcaagag tccaaccact tacccatgat tttccggtag gagcaaatgc
      421 acctctgtaa gctaccggta tcataccttt cgcttgtggc gctgcttcat ttgaatagaa
      481 tggattagca gttccactta ctgtagcagc tatagaagaa ggtacaacat catatttggt
      541 agttacagca ttagttgcag tattcatagc ccacacatat gcaaatccga atttaggatc
      601 taaagaacct gcaggaataa caatgttttt atcggtagta gtaaattgtg ttaaatctgc
      661 agcagaagga gctgttggag ttacagattg aatagtagca tttttacccg atactaaagc
      721 acctatagac ataggcatat cgcaaccaat aaatgtattg ttttttactt ttaatgaatt
      781 aggtatagac aatgttaatt ctgtagcagt atttgaccag ttttgatatg cattaccttc
      841 agtagcatta gtagctaaat gtaatcctga acggaagtta acaaatactg agttatatat
      901 agaaccttgt gttctatctt tagccataat accagcaaca cctgtgttat cagcagttgg
      961 agtaaaatta ccgttactta ccaacgttgc attgtagatt gttggattag aacgttttgt
     1021 tttgtcaaaa gttgttgtta aatttttatc atcaccatca ccctctaaac cattatcaga
     1081 agatccaggc cataagttac catttgtatt gtttggttcg tgaattccga atataaactg
     1141 agctgtacca ttccaaccta agtcgtagtc aagcatatca tcgcaaccaa acatagatgt
     1201 aatatatcgt aaattaacag ttcccccgaa tatttcaata tcatcatcgt catttgatat
     1261 tacttcaata ttttctattg tagtaccacg accaactgat gctaatgaaa ggccatttaa
     1321 ttcgtttcct tgtgcgattt cgcaacctgc gtgacgaata gaaacataac gcattatacc
     1381 tgagttatca tcgtcgttaa attgctcagc accattacct ttagaattat ttatatcagc
     1441 accataccag ttgtaaggat tcaatacgtc gaaaccttcc acaacaccta aaccagcagc
     1501 aatacagtta ccattctcac caccgtttat acagttttgt gcttgaccta ataatgcaat
     1561 accaccccat ttacctacgt ttccaatagg atatgtacca tctaagttgt cagcttctgc
     1621 cgtgaataca attggacatg tttctgtacc acttgcaaaa attttaccac cacgtgatac
     1681 gattaaagca gcagcgtttt cagcaacacc accatgtgct ctacctttaa taagagttcc
     1741 cggttgaatt gttaaagatt t
//
LOCUS       DAOTOW010000524         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52549_length_1761_cov_2.715709, whole genome shotgun sequence.
ACCESSION   DAOTOW010000524 DAOTOW010000000
VERSION     DAOTOW010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52549_length_1761_cov_2.715709"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="P5243_08840"
     CDS             <1..595
                     /locus_tag="P5243_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19598.1"
                     /translation="LTDLINSTLCVGVLYSFGAVGSTVTMFSNNYIQSYLISFHYAKQ
                     IFTKASVSVSGIYSCSQSEEFNSNCNSIIPQISATYKPYSELLLGFTIVQPIYYRLKQ
                     TKYFEEFECAASYSGIPNVVVTIATKKSYNDFLITTVGIDYTVHTSLVFSASVANQYR
                     PLSLGIGYRISRCIFQYATSVHSFLGFSHTVSFLYSL"
     gene            630..1415
                     /locus_tag="P5243_08845"
     CDS             630..1415
                     /locus_tag="P5243_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRS19599.1"
                     /translation="MKNCLKFLVFSILVIFAASCTSNKNLVYLENIPQGTSDKISSTK
                     QTLEYKLQSGDVVYVKILSINKELNELFNVDNSNNQNAYINETSINLRGFTIDNNGYI
                     RLPIIDTVKIAGMQAFEAERLIQTKVNEYFKNATVVLKPLNNKISVLGEVNRPGQFVI
                     YRNDVNILEAIAMAGDVNQFADKKKILIIRTIGTTNVTYRVDMTDTKILSNKDFYLLP
                     NDIVIVEPLKLKSLRVNTPTISLVLSGIGTIVNALTLYSVFNK"
     gene            1430..>1761
                     /locus_tag="P5243_08850"
     CDS             1430..>1761
                     /locus_tag="P5243_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRS19600.1"
                     /translation="MNSVLQNQEEENINIKKYIQLFLDNWRWFVATIIITVSIAFLVN
                     RYSAPKFESQNTVYLTEKEDAMGAMSTIMKEFGSFGSKTQNIENQMGILTSYSLTRKT
                     LERLNYQVS"
BASE COUNT          635 a          232 c          272 g          622 t
ORIGIN      
        1 acttaccgat ttgataaact ctaccttatg tgtgggagta ctttattcat ttggtgcagt
       61 tggttcaaca gttactatgt tttctaataa ttatatacaa tcatatctta ttagttttca
      121 ttatgctaaa caaattttta ctaaagcatc agtttctgtt tcgggtatat attcttgttc
      181 acaatcagag gaatttaata gtaattgtaa ttctattata ccacaaatat ctgcaaccta
      241 taaaccatat agtgaattgc ttttaggatt taccatagtg caacctattt actatagact
      301 caaacaaact aaatactttg aagagtttga gtgtgctgct tcatattcgg gaatacctaa
      361 cgttgtagtt actattgcaa caaaaaaatc ttacaatgat tttttgataa caactgttgg
      421 tattgattat acagttcata catcgcttgt gttttctgct tcagttgcaa atcaatacag
      481 gccattaagt ttaggaattg gatatagaat ttcacgttgt atatttcaat atgcaacttc
      541 ggttcattca tttctcggat tttcacatac agtaagtttt ttgtatagtt tgtaattatt
      601 ttttgttata attgcaaaaa aaatgtacta tgaagaattg cttgaaattt cttgtgttta
      661 gtatacttgt gatttttgct gcaagttgta cttcaaataa aaatttagtg tatttagaaa
      721 atataccaca aggaacttcc gataaaatat catcaacaaa acaaacactt gaatataaac
      781 tgcaatcggg tgatgtagta tatgttaaga ttttgagtat aaacaaagaa ctgaatgaat
      841 tgtttaatgt agataattca aataatcaaa atgcatatat caatgaaaca tctattaatt
      901 tgcgtggttt tactatagat aacaacggat atattcgatt gcctataata gatactgtta
      961 aaattgcagg aatgcaagca tttgaagcag aacgtttaat acaaactaaa gtaaatgagt
     1021 attttaaaaa tgcaactgta gtattaaaac cattgaataa caaaatttct gtgttgggcg
     1081 aggttaacag acccgggcaa tttgtaatat acagaaatga tgttaacata ttagaagcta
     1141 ttgcaatggc cggtgatgta aatcaatttg cagataaaaa gaaaatactt ataatacgta
     1201 caataggcac tacaaatgtt acttatagag ttgatatgac tgatacaaaa atattgtcaa
     1261 ataaagattt ttatttgttg cccaatgata tagttatagt tgaaccattg aaattgaaat
     1321 cgttgagagt taatactcca acaatatcac ttgtattgag cggtattggt accatagtaa
     1381 atgcattaac attgtattct gtttttaata aataatttat tccttttata tgaatagtgt
     1441 acttcaaaat caagaagaag aaaatataaa tattaaaaaa tatattcaat tatttcttga
     1501 taactggcgt tggtttgttg ctaccataat tataactgtt agtatcgctt ttttggtaaa
     1561 cagatattct gcgccaaaat ttgaatcaca aaatacagtg tatttaaccg aaaaagaaga
     1621 cgctatgggt gccatgagta ccattatgaa agaatttggt tcgtttggaa gcaaaacgca
     1681 aaatattgaa aatcaaatgg gaattcttac atcatattct cttacgagaa aaacattaga
     1741 acgattaaat tatcaggttt c
//
LOCUS       DAOTOW010000525         1759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52622_length_1759_cov_3.282277, whole genome shotgun sequence.
ACCESSION   DAOTOW010000525 DAOTOW010000000
VERSION     DAOTOW010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52622_length_1759_cov_3.282277"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="P5243_08855"
     CDS             <1..848
                     /locus_tag="P5243_08855"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19601.1"
                     /translation="VATGGTASTTAPTPSTAAAGSTTYYVSQTVNSVESPRAEIVVTV
                     TASPSAPTVTTPVTYCQGATATALTATGTALKWYTVATGGTASTTAPTPSISAAGSTT
                     YYVSQTVNGCESPRASIVVTVNAIPAAPAVVSPVVYQTGATSTALTATGTALKWYTVA
                     TGGTASTTAPTPSTATVGTTNYYVSQTVNGCESSRALIAVQVSAVVQKVQLKAGWNMI
                     GCPISGSTPIETALGSIWSHVEQVKNMDAFYIKTNNPALNSLQTIEWGRGYLIKVTNA
                     CELTW"
     gene            complement(1059..>1759)
                     /locus_tag="P5243_08860"
     CDS             complement(1059..>1759)
                     /locus_tag="P5243_08860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS19602.1"
                     /translation="SWIFPKLTHCMTVCESIAHIYEQKYSVPVIVVRNVPLLQKHTMS
                     TNVQLPTIPQPHIILYQGAVNVGRGIELMIQAMEYINNTALLIIGSGDKLEELKEFAK
                     QSKNASCIYFTGRIPFQQLPAYTNIATIGFSLEEDIGLNYKYALPNKIFDYIHAHKPI
                     VVSDLPEMSSIIQTYNCGEIIYNRNPQAIGQQITNMLSNTDLLQLYAQNSAIASKELC
                     WEREEQKLLQCYNI"
BASE COUNT          579 a          316 c          331 g          533 t
ORIGIN      
        1 ccgtagcaac cggaggtaca gcaagtacca ctgcaccaac gccaagtact gcagctgcgg
       61 gttctaccac atattatgtg tcgcaaacag tgaattcggt agaaagtcca cgagcagaaa
      121 ttgttgtaac agtaacagca tcacctagtg caccaactgt aacaacaccg gtaacatatt
      181 gccaaggagc tacagcaact gcgttaacgg caaccggaac agcactgaaa tggtatactg
      241 tagcaaccgg aggtacagca agtaccacag caccaacgcc aagtatatca gctgcgggtt
      301 ctaccacata ttatgtgtcg caaacagtaa atggttgtga aagtccacgt gcttctatag
      361 tagtaacagt gaatgcaata cctgcagctc ctgccgtagt aagtcctgta gtatatcaaa
      421 caggagcaac gtctactgct ttgactgcca ccggaacagc actaaaatgg tacacggtag
      481 caacaggagg cactgcaagt actactgcac ctacgccaag tactgcaact gtaggaacta
      541 ctaattacta tgtatcgcaa acagtaaatg gttgcgaaag ttcacgtgct ttaattgcgg
      601 tacaagtaag tgcagtagtt caaaaagtac agctaaaagc tggttggaat atgataggat
      661 gtccaataag tggaagtact ccaatagaaa cagcattggg aagtatttgg tcacatgttg
      721 aacaagtaaa aaatatggac gcattctata taaaaacaaa taatcctgcc cttaattcat
      781 tacaaaccat agaatgggga agaggatatt tgataaaagt aacaaatgcg tgtgaattaa
      841 cctggtaata attataaaaa attaaatggt tatatatgac aatagatagt taaaaaagtc
      901 tccaatcagt acaatggggt tacgcatatt taaataagtt atcgctccct gtgaattaac
      961 ttggtaatac atttcttcct ttacctcatt gtaccattca tatctccaaa ttctcagtaa
     1021 aaaaggccga tacacccgta atcggccttt ttaatttttt atatattata acactgtagc
     1081 aatttttgtt cttcacgttc ccagcataat tctttggaag ctatggcaga attttgagca
     1141 tataattgta ataaatctgt gttagaaagc atgtttgtta tttgctggcc aattgcttga
     1201 ggatttctat tgtatataat ttctccgcaa ttatatgttt gtataattga actcatttca
     1261 ggtaaatctg aaactacaat aggtttatga gcatgaatat agtcgaaaat tttattaggt
     1321 aaggcgtatt tatagtttag tcctatatct tcttccaatg aaaagcctat agttgctata
     1381 ttagtatatg caggcaattg ttgaaaggga attcttcctg taaaatagat acatgatgca
     1441 ttttttgatt gttttgcaaa ttctttcaat tcttcgagtt tgtctccact cccaataatt
     1501 agtaacgctg tattgttaat atattccatt gcttgtatca tgagttcaat acctctgccc
     1561 acatttactg caccttgata aagtatgata tgtggttgtg gtatagttgg taattgaaca
     1621 ttagttgaca tagtatgttt ctgtaataat ggaacattac gaactacaat aacaggaaca
     1681 gaatattttt gttcgtatat atgagctata gattcacaaa ctgtcatgca atgtgtgagt
     1741 tttggaaaaa tccaagatt
//
LOCUS       DAOTOW010000526         1755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52792_length_1755_cov_2.880000, whole genome shotgun sequence.
ACCESSION   DAOTOW010000526 DAOTOW010000000
VERSION     DAOTOW010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52792_length_1755_cov_2.880000"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="P5243_08865"
     CDS             <1..343
                     /locus_tag="P5243_08865"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRS19603.1"
                     /translation="KKIQDKPAKQLKDTRNTREITYEMFCSGMSIEDIAKQRDFTVST
                     IENHIAYFVAEGMIDIRELVEKETLELVSKTIVTLQTKSLKEVKEAVPEHITYSDIRF
                     CMAWLEHSNML"
     gene            complement(346..1056)
                     /locus_tag="P5243_08870"
     CDS             complement(346..1056)
                     /locus_tag="P5243_08870"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRS19604.1"
                     /translation="MSHSIVQTIRNSFASMQNEAIQMSNCRFFKEPIQTYGIRSLDIE
                     KIAKQYKTHIQKCNKQIVFDICKDLWHSGYIEEGFVACHFTQYIQKKLDADDMYIFSE
                     WIDTYISNWATCDTFCNHTIGDLLQNFPEILPHIKQWVHSNNMWKRRASAVSLILPAR
                     RGLYKETVFEIAEILLHDSEDLVQKGYGWMLKETSKPYCNDVFNFIMKHKHSMPRTAL
                     RYAIEKMPPELRSKAMEK"
     gene            complement(1059..>1755)
                     /locus_tag="P5243_08875"
     CDS             complement(1059..>1755)
                     /locus_tag="P5243_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRS19605.1"
                     /translation="VIRWTDIIKNMYLPEDVTYDIILQQDGYLDKDLSTVHDIPANQR
                     PINQHWSWDRILRSCYIKQADVVQGLYVFESDFDIETITRNFNFYEARCVHESSLSPC
                     IHSVVASKIGNADKAYELYLRTARLDLDDYNKEAHEGLHITSMAGTWLAIVQGFAGLR
                     WNQYGLSLNPQIPKHWKSFSFKIIYRGALITITIKAKKATILLESGFVSHITVCNQKY
                     SLQVGSELHINLQ"
BASE COUNT          578 a          268 c          274 g          635 t
ORIGIN      
        1 aaaaaaaata caagataaac cggcaaaaca attaaaagat acccgtaata ctcgtgaaat
       61 tacatacgaa atgttttgtt caggtatgtc tattgaggat attgctaagc aaagagattt
      121 tactgtttct acaattgaaa accatattgc ttattttgtt gcagaaggta tgattgatat
      181 acgcgaactc gtagaaaaag aaacgttaga attagtttca aaaactattg ttacattgca
      241 aacaaaatct cttaaagaag ttaaagaggc agtgcccgaa catataacgt attccgatat
      301 acggttttgt atggcttggt tagaacacag taatatgttg taattttatt tttccattgc
      361 tttggagcgt aattctggag gcattttttc tattgcatat cttagtgcag ttcgtggcat
      421 agaatgttta tgtttcatta taaaattaaa aacatcgtta caatatggtt ttgatgtttc
      481 tttcaacatc caaccgtatc ctttttgaac taaatcttca gaatcatgca ataaaatttc
      541 agcaatttca aatacagttt ctttatataa tcctctgcga gcaggtaata ttagtgaaac
      601 agcagaagct ctgcgtttcc acatattgtt agaatgtacc cattgtttaa tgtgaggaag
      661 aatttccgga aaattttgta gtaaatcgcc tatagtatga ttgcaaaatg tatcacaagt
      721 tgcccaatta gaaatatatg tgtctatcca ttcagaaaat atatacatat catctgcgtc
      781 taattttttt tgaatatact gtgtaaaatg acaggcaaca aatccttctt caatatatcc
      841 tgaatgccat aaatctttgc atatatcaaa aacaatttgt ttgttacatt tttgaatatg
      901 agttttatat tgtttagcaa ttttttctat gtccaaactt ctaattccat acgtttgtat
      961 tggttctttg aagaatctac aattgctcat ttgtatagct tcattttgca tagatgcaaa
     1021 tgaatttcga atagtttgaa caattgaatg agacattgct attgtaaatt aatatgtaat
     1081 tcagaaccaa cttgtaatga gtatttttga ttgcaaaccg taatatgaga tacaaatcct
     1141 gattcaagta aaatagttgc ttttttagct ttaattgtta tagttattaa agctccacga
     1201 tagattattt tgaatgaaaa tgatttccaa tgttttggaa tttggggatt tagacttaat
     1261 ccatattgat tccatcgtaa tcctgcaaat ccctgaacta tggcaagcca tgttcctgcc
     1321 atacttgtta tatgtaatcc ttcgtgagct tctttattat aatcatctaa atctaatcga
     1381 gcagttctta aatataattc gtatgctttg tctgcatttc caatttttga ggcaactacc
     1441 gaatgaatac aaggtgataa agaagattcg tgtacacatc gagcttcgta aaagttaaaa
     1501 tttcgagtga ttgtttctat gtcaaaatct gattcaaaaa catataatcc ttgaactaca
     1561 tctgcttgtt taatatagca tgatcgcaaa attctatccc atgaccaatg ttgatttatt
     1621 gggcgttgat tagcaggaat atcatgtacg gttgataaat ctttgtctaa atatccatct
     1681 tgttgcaaaa ttatgtcata ggttacatct tcgggcaaat acatattttt tataatatca
     1741 gtccagcgta taacc
//
LOCUS       DAOTOW010000527         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52824_length_1754_cov_4.181283, whole genome shotgun sequence.
ACCESSION   DAOTOW010000527 DAOTOW010000000
VERSION     DAOTOW010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52824_length_1754_cov_4.181283"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="P5243_08880"
     CDS             complement(<1..652)
                     /locus_tag="P5243_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19606.1"
                     /translation="MKKFLLLIYVSLFSYIGFAQQYANNWIQYSQKYYRIPITSEGIY
                     KVTYNDLVSYGISVGDFDPRNLQIIHNGKVLPLFVSGESDFVFNVQDYFEFYAPGRNT
                     GWLDTAMYMKNNPLNPDYSLYNDTASYFLTFANTLTSPRYDTTRNTNFSSYTAQSYCL
                     RKVRQNFTAVYNSSAHAPYMYEGEGWCDNFFNMGASTDKTLSTPQYANVGQPYSLRF"
     gene            complement(690..>1754)
                     /locus_tag="P5243_08885"
     CDS             complement(690..>1754)
                     /locus_tag="P5243_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19607.1"
                     /translation="TLGDEVTPFYDARDSLLYFSSEWHTSLGGFDIFSAKGNLKTDKW
                     SAPANIGKPMNSSYNDLYYQYSRDSLRAYWVSNRTESQRLISKAYSNDIYYHPLIRKS
                     VARITDLVPIYLYFDNDQPDPRSRDTLTDKDYESLYQAFMAKKQEYIIEFTKNSPSER
                     YDYDVKNVETFFADLQKEYERLFLFAQLMEILLKDGQDIVVVFKGYASPVGNTAYNEI
                     VAKKRISCIQNFFYEFHDGVLNQYLKNEPNTGKGSLKYGHQPIGEIKMEDTFYTSSGE
                     QVSAISDRKQKWMSVYSPAAAYQRKIEIVAVTIEYEDELFNKIRAEVETQKKTVEDDG
                     TILENDSQDARSKRDIIREK"
BASE COUNT          554 a          298 c          294 g          608 t
ORIGIN      
        1 caaatcgtaa agaatatggt tgacctacgt ttgcgtattg tggagttgaa agagttttgt
       61 cggtacttgc acccatatta aaaaaattat cgcaccaacc ttcgccttca tacatatagg
      121 gagcatgagc cgatgagtta tatactgcag taaaattttg tcgtactttt cgcaaacagt
      181 atgattgtgc tgtatatgaa ctgaaattgg tgttacgtgt tgtatcgtag cgggggcttg
      241 taagggtatt ggcaaaagtt aaaaagtatg aagcagtatc attgtataaa ctataatccg
      301 gattgagagg gttgtttttc atatacatag cggtatcgag ccaacctgta tttcgccccg
      361 gggcataaaa ttcaaaataa tcttgaacat taaatacaaa atctgattct ccgcttacaa
      421 ataatggtaa aactttgcca ttatgaatta tttgaagatt tctggggtca aagtcaccaa
      481 cagatatgcc gtaagaaaca aggtcgttgt atgtaacttt gtaaattcct tctgatgtta
      541 tagggattct ataatatttt tgagaatatt gtatccaatt atttgcatat tgttgagcaa
      601 acccaatata tgaaaacaga cttacatata taagtaataa aaattttttc ataattgaat
      661 cctccacaaa tcttagacgc acaaagctcc tatttctcac gtattatgtc tcgttttgaa
      721 cgagcgtctt gagaatcatt ttctaaaata gttccatcgt cttctactgt ttttttctga
      781 gtctcaactt cggcacgtat tttattaaat aattcatctt catactctat agtaactgct
      841 acaatttcaa tttttctttg atatgcagca gcagggctgt ataccgacat ccatttttgt
      901 tttcggtcag aaatagcact tacttgttct cctgaacttg tatagaatgt atcttccatt
      961 ttaatttcac caataggttg atgaccgtat tttaagcttc ctttacctgt attaggctca
     1021 tttttaagat attgattgag tactccatca tgaaattcat agaagaaatt ttgaatacat
     1081 gaaatacgtt tttttgcaac aatttcattg tacgcagtat ttccaaccgg acttgcatat
     1141 cctttaaata caacaactat gtcttgaccg tcttttaata atatttccat aagttgagca
     1201 aataaaaata gtcgctcata ttctttttgt aagtcagcaa agaaagtttc aacatttttt
     1261 acatcataat cataccgttc tgatggcgaa ttttttgtaa attctattat atattcttgt
     1321 ttttttgcca taaacgcctg atacaatgat tcgtaatctt tgtcggtaag cgtatcgcga
     1381 gaacgaggat ctggttgatc gttatcaaaa tacagatata ttggaactaa gtctgtaata
     1441 cgagcaacag atttacgaat aagaggatga taataaatat cattgctata tgctttactt
     1501 ataagacgtt gtgattctgt tcggttcgaa acccaatatg ctcgtaaaga gtcgcgcgaa
     1561 tattggtaat ataaatcatt atagcttgag ttcattggtt taccaatgtt agcaggagcc
     1621 gaccatttat cggtttttaa attgccttta gccgaaaaaa tatcaaaacc acctaaacta
     1681 gtgtgccact cgctactaaa ataaagtaat gaatcgcgag catcataaaa aggtgtaact
     1741 tcatcaccta aagt
//
LOCUS       DAOTOW010000528         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52989_length_1750_cov_3.895575, whole genome shotgun sequence.
ACCESSION   DAOTOW010000528 DAOTOW010000000
VERSION     DAOTOW010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52989_length_1750_cov_3.895575"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1750)
                     /locus_tag="P5243_08890"
     CDS             complement(<1..>1750)
                     /locus_tag="P5243_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19608.1"
                     /translation="SSDYWADSYGLVNNSFGKNTITIGVLSLDGLDNKGKLYSNLSSI
                     PSVADIVTSQPINLKSYKKYYKSDKLYIKNNGFQLLTNDYYVYQSCNNTFESVSKGFL
                     FAAGDTIYHKVVNDYIPVIDSIFDENFQYILGTYTFEQQWYNYTIQDSIALSFYYQSG
                     GNADKPESTDSLVLEFYTPFQREGIFINEITTNWIEIYNATDTVISLYNWFIVTDTIT
                     KCINQNVLEKCKIKNTKFIAPYSHAIIFAKDVGITKFTHSLCMLYNPDTVMVDSIELN
                     EKITDDFSYARIPDGNIQWSYSVEQTPEYPNSAWNRIWATSNDTKDFFSSVFIPIVSS
                     NYLQKGFRFRFKNYVSLSNDVSHARNEDIWNIDMVWLDVQRKKDIINTPDVAFVQQIA
                     PLYNQFSAIPISHFSVVQPTNFRLTIDAKFNNFDSLYRKVKFGFALKKHHVSDELLFQ
                     TYETDIPPYTLAEERDVLSDWNVDFIDFMKQDIGVFDETKYEFQYYFTDNNNIAYKQH
                     RWNDTSRVNLVMANYYAYDDGIPEAGYGLREAPMGRVAYKYSVLVPDTLKAIDIYFNP
                     NLVTAPPIFNLCIWSMN"
BASE COUNT          639 a          269 c          234 g          608 t
ORIGIN      
        1 attcatagac catatacata aattaaaaat tggtggcgca gttactaaat taggattaaa
       61 ataaatatca atagctttta acgtatcggg aactaataca gaatatttat atgctacacg
      121 tcccatagga gcttcacgta aaccataacc ggcttcggga attccatcat cgtatgcata
      181 ataatttgcc attacaagat ttactcggct tgtatcattc catctatgtt gtttgtatgc
      241 tatgttatta ttatctgtaa aataatattg aaactcatac ttagtttcat caaacacacc
      301 tatgtcttgt ttcataaaat caataaaatc aacattccaa tctgaaagca catctcgttc
      361 ttctgccaag gtataaggag gtatatctgt ttcatatgtt tgaaaaagta actcatcaga
      421 aacatgatgt ttttttagag caaaaccaaa ttttactttt ctatatagtg aatcaaaatt
      481 attaaacttg gcatcaattg ttaatctaaa attggttggt tgtaccacag aaaaatgaga
      541 aattggaatt gcagaaaatt gattatacaa aggagctatt tgttgaacaa acgcaacatc
      601 aggcgtatta attatatctt ttttcctctg aacatcaagc cataccatat caatattcca
      661 aatatcttca tttcgagcat gactaacatc attacttaaa ctcacataat ttttaaatct
      721 gaatcgaaat cctttttgta aatagtttga tgatacaatt ggaataaata ccgacgaaaa
      781 aaaatctttt gtatcgttag aagttgccca tattctattc caagcagaat ttggatattc
      841 aggcgtttgt tcaactgaat aactccattg aatatttcca tcaggaatgc gagcatacga
      901 aaaatcatca gtaatttttt cattcaattc aatactatca accattactg tatcaggatt
      961 atacaacata cataatgaat gagtaaattt tgtaatgccc acgtctttag caaaaatgat
     1021 agcatgtgaa tatggtgcta taaatttagt gtttttaatt ttacattttt ctaaaacatt
     1081 ctgatttata catttcgtaa tagtatcagt aacaataaac caattgtata atgaaataac
     1141 tgtatcagta gcattatata tttctatcca attcgttgta atttcattta taaaaattcc
     1201 ttcgcgctga aaaggggtat aaaattccaa cactaaacta tcagtagatt cgggtttatc
     1261 tgcattacca ccagattgat aataaaaact aagagctata gaatcttgaa ttgtataatt
     1321 gtaccattgt tgttcaaaag tgtatgtgcc aagtatatat tgaaaatttt catcaaaaat
     1381 tgaatctatt acaggaatat aatcatttac aactttatga tatatagtat ctcctgcagc
     1441 aaacaaaaag cctttagaaa cagattcaaa tgtattatta catgattgat atacataata
     1501 atcatttgta agcaactgaa atccattgtt ttttatatat aatttatcac ttttgtaata
     1561 ctttttatat gatttaagat taattggttg tgaagttaca atatctgcaa cagagggtat
     1621 agaagataga tttgaataca attttccttt attatcgagg ccatcaagag agagaacacc
     1681 aatagtaatt gtatttttac caaatgaatt atttactaat ccataggaat ctgcccaata
     1741 atcagaagaa
//
LOCUS       DAOTOW010000529         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_52992_length_1750_cov_3.588791, whole genome shotgun sequence.
ACCESSION   DAOTOW010000529 DAOTOW010000000
VERSION     DAOTOW010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52992_length_1750_cov_3.588791"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="P5243_08895"
     CDS             <1..1110
                     /locus_tag="P5243_08895"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRS19609.1"
                     /translation="AIAYNIIVNINAILPAAEKNTHLKQSEKDIIKGEALTLRAIIYY
                     DLVRLFAHDYSITSGIAGANGKGGHAGIPLITSPISLDSAKKVSRSSVNTVFNQILTD
                     ALHADSLLQQQTFSPLRCNQKTAQTLVANIYLHLKNYNKAIEYANKALESIQLEQNNT
                     YIQSWSQQYSTESIFSVCMTPTDNPGTNSIGHMLSPKGYGGIVPSEDLLKIINTTDIR
                     SQFIQKYQEYYFIKFPEINNTLGINNIPVFRASELYFILAEAFANKGKTVPGFYTLSQ
                     QALQSIKQRAYNSQEPVTLTGDNLLNEIYEEYRKEFAFEGKRLFQLKRLHQDIARKNC
                     NTNNCILSCNNYKYTFPIPYSEIQTNKNITQNPGY"
     gene            1110..>1750
                     /locus_tag="P5243_08900"
     CDS             1110..>1750
                     /locus_tag="P5243_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19610.1"
                     /translation="MRILYIILCICIGFISCKENSYINPIIDTTYIEFPTNTIHQTLL
                     YTETSPYTLNLPFQVFGKSLNQNDTIVVRVTSNYFIKNTDFWYDSIQIITPTTIIDTI
                     HIHIKNTKMWEGGTFSLDCKLISKNNKIHTSKNYQNCTLFFTRESFISYFTGNYSCFE
                     NSTESTYPVTFIQSQNDSVLYNTNFWDFPLIGQNVPYIFRKDQDKTIIIPETV"
BASE COUNT          676 a          291 c          211 g          572 t
ORIGIN      
        1 gctattgcat ataatattat tgtaaatata aatgctattt tgcctgccgc tgaaaaaaat
       61 acacacctta aacaatcaga aaaagacatt ataaaaggag aagctcttac attacgagct
      121 attatttatt atgatttagt tcgtcttttt gctcatgatt attctattac ttcaggcatt
      181 gccggtgcta atggtaaagg cgggcatgcc ggaataccgc ttataacctc accaataagt
      241 cttgacagtg caaaaaaagt ttctcgaagt tctgttaata cagtattcaa tcaaatactt
      301 acagatgctc tccatgcaga tagtttactt caacaacaaa cattttcacc gcttcgttgt
      361 aatcaaaaaa cagcacaaac tcttgttgca aatatatatt tacacctgaa aaattataat
      421 aaagctattg aatatgccaa taaagcatta gaatccattc aactcgaaca aaacaatacg
      481 tacattcaat cttggagtca acaatatagt accgaaagca ttttttcggt atgtatgaca
      541 ccaacagaca acccaggaac caatagtatt ggtcatatgc tttctccaaa aggatatgga
      601 ggaatagttc cttccgaaga cttactaaaa ataataaata caactgatat tcgatctcaa
      661 tttattcaaa aatatcaaga atattatttt ataaaattcc ctgaaataaa taatacatta
      721 ggaattaata atatacctgt ttttagagca agtgaattat attttatact agcagaagcc
      781 tttgctaata aaggcaaaac tgttccaggt ttttatacat tatcgcaaca agcattacaa
      841 tcaataaaac aacgagcata caatagtcaa gaacctgtta cccttaccgg agataattta
      901 cttaatgaaa tttacgaaga atacagaaaa gaatttgcat ttgaaggtaa acgtttattt
      961 caactcaaac gccttcatca agatattgcg agaaaaaatt gtaatacaaa taactgtata
     1021 ttatcatgta acaattataa atacacattc cctattccat attcagaaat tcaaacaaat
     1081 aaaaatataa ctcaaaatcc gggatactaa tgagaatttt atatatcata ctatgtatat
     1141 gtataggttt tatttcatgt aaagaaaatt cttatataaa ccctattata gatactacat
     1201 atattgaatt cccaacgaat actattcatc aaactttatt gtatacagaa acgtcacctt
     1261 atacattgaa tttgcctttt caagtttttg gcaaatcact caatcaaaat gatactatag
     1321 ttgtaagagt tacatcaaac tattttatca aaaacactga tttttggtat gattctatac
     1381 aaataattac ccctactact ataatagata ctattcatat tcatatcaaa aatactaaaa
     1441 tgtgggaagg tggtacattt tctttagatt gtaaattaat ttctaaaaac aataagattc
     1501 atacatcaaa aaattatcaa aattgcactc tattttttac acgagaatca tttatatcat
     1561 actttaccgg taattatagt tgctttgaaa attctacaga atctacatat cctgttactt
     1621 ttattcaatc tcaaaatgat agtgttctat ataataccaa tttttgggat ttccctttaa
     1681 ttggtcaaaa tgtaccatat atatttagaa aagatcaaga taagacaatc ataattccgg
     1741 aaacagtatg
//
LOCUS       DAOTOW010000530         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53040_length_1749_cov_3.113341, whole genome shotgun sequence.
ACCESSION   DAOTOW010000530 DAOTOW010000000
VERSION     DAOTOW010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53040_length_1749_cov_3.113341"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="P5243_08905"
     CDS             complement(<1..1095)
                     /locus_tag="P5243_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRS19611.1"
                     /translation="MKKLNFKIIFQIIGIFTIVEAIAIFLCVIATVYYNENYKAILYA
                     SGITGSIGIVLYSLFKNAPKDIGKREGYIIVTFAWLFFSIFGALPYVISGSMPNFADA
                     FFESMSGFTTTGASILSDIEALPKDILLWRSLTQWLGGMGIIVLSLAIIPLLGIGGMQ
                     LYAAEVPGPTKDKIHPKVKDTAQRLYLIYVSFTLLETLLLMIGGMSFFDALNHSFTTM
                     ATGGFSTYNTSLVHVSPYIQYVVIAFMFLAGVNFTLSYFAFHGKFERVWNNEEFRSYT
                     LIIILFTCIVACIIGYQQYVANHSIVGIEKIIRDSLFQVVSLCTTTGFASANYLVWPA
                     AAIMLCFMLLFFGGSAGSTGGGMKVVRIVIL"
     gene            complement(1101..>1749)
                     /locus_tag="P5243_08910"
     CDS             complement(1101..>1749)
                     /locus_tag="P5243_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRS19612.1"
                     /translation="LGASRTGRATAKYLQNLFDVKLIELNEQRAKIIANELTNVLVIN
                     GDGRSTDLLVEEGIRNTDVFIATTGNSETNILSCYLAKSVGVKRTIAEVENFDYIALA
                     RDMGIDTVINKKTIAANSIHKYTLGEKTAITSSRYLTETEAQVLEIYIKPGMKITEQQ
                     LKFLDFPRAAVIGGVVRGKDAFIPKGNTQIKNGDKVLVFSMPNAIGDIKKYFESE"
BASE COUNT          620 a          341 c          225 g          563 t
ORIGIN      
        1 aagtattact atacgtacca ccttcatacc accaccggta gaacctgcac taccaccaaa
       61 aaaaagcaac ataaaacata acataattgc tgctgcaggc catactaaat aattagctga
      121 cgcaaaaccg gtagtagtac ataatgaaac tacttgaaat aaagaatctc gtataatttt
      181 ctcaatacct acaattgaat gatttgcaac atactgttga tatcctataa tacatgctac
      241 aatacacgta aataaaatta taattaaagt ataacttcta aattcttcat tattccatac
      301 tcgctcaaat ttaccatgaa acgcaaaata tgataaagta aaattaacac cagctaaaaa
      361 cataaacgca ataacgacat actgaatata gggtgaaaca tgaaccaaac ttgtattata
      421 cgttgaaaaa cctccggtag ccattgtagt aaacgaatga tttaaagcat caaaaaatga
      481 catacctcca atcatgagta acagtgtttc taatagtgta aatgacacat aaattagata
      541 caaacgttgg gctgtatctt ttactttagg atgaatttta tctttagttg gacccggtac
      601 ttcggcagca tataattgca tacctccaat tcctaaaagt ggtataatag ccaatgaaag
      661 gacaattata cccataccac caagccactg agttagacta cgccacaaca agatatcttt
      721 gggcaatgct tctatatcag ataatataga tgcccctgtt gtagtaaacc ctgacattga
      781 ttcaaaaaat gcatctgcaa aattgggcat agaacccgaa attacatatg gcagagctcc
      841 aaaaattgaa aaaaataacc aagcaaatgt tacaataata taaccttctc gttttccaat
      901 atcttttgga gcgtttttaa ataaagaata taaaactatt cctatactac cggtaatacc
      961 acttgcatac aaaattgctt tataattttc attataataa acagtagcaa ttacacacaa
     1021 aaatatagca atagcttcta ctattgtaaa aattccaatt atctgaaata ttattttaaa
     1081 attcagtttt ttcaccggct ttattcactt tcaaagtatt tttttatatc gcctatggca
     1141 tttggcattg aaaatactaa tactttatct ccatttttta tttgagtgtt cccttttgga
     1201 ataaaagcat ctttacctcg cacaacacct cctataacag cagctctcgg aaaatcaaga
     1261 aattttaatt gttgttcagt aattttcatt cccggtttta tataaatttc aagcacttga
     1321 gcttctgttt ctgtaagata acgcgacgat gtaattgcag ttttttcgcc caatgtatat
     1381 ttatgaatac tattggcagc aatggttttt ttatttatta ccgtatctat tcccatatca
     1441 cgggccaaag caatataatc aaagttttca acctcagcaa ttgtacgctt tacaccaaca
     1501 ctttttgcta aataacacga taaaatattt gtttcggaat tacccgttgt agcaataaac
     1561 acatcggtat ttctaatgcc ttcttctacc agcaaatcgg tacttctgcc atctccattt
     1621 attacaagca cattggtaag ttcatttgct ataatttttg cccgttgttc atttaattct
     1681 ataagcttta catcaaataa attttgtaaa tatttagcag tagcgcgtcc tgttctactt
     1741 gcacctaaa
//
LOCUS       DAOTOW010000531         1747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53126_length_1747_cov_3.921395, whole genome shotgun sequence.
ACCESSION   DAOTOW010000531 DAOTOW010000000
VERSION     DAOTOW010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53126_length_1747_cov_3.921395"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="P5243_08915"
     CDS             <1..303
                     /locus_tag="P5243_08915"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRS19613.1"
                     /translation="FTPKNTLNPEWFLFKHQDKVIHSVLYGVLMILFLYNYSSYSQLQ
                     KRVFVYASVSFICISFCIEILQPILSQRSFEFADILSNAIGLFLGMAGYRLIFYKR"
     gene            493..1077
                     /locus_tag="P5243_08920"
     CDS             493..1077
                     /locus_tag="P5243_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRS19614.1"
                     /translation="MNLDIFNITHNHVEPAVGKVLLSVPFINDTYFSRSVILLTLHDS
                     EGSVGYILNKRIPMFLHELVDDIPRSKFTVCIGGPVANDTLQMLHSLGHMIPDSIQIK
                     PGLFWGGNFDAVRSLIKKQLIHPHEIRFFMGYSGWGKLQLHDEIQANSWVVTDIGVDT
                     ILTHSEEKLWNATLVKMGSKYKAWADFPRDPSLN"
     gene            1092..1631
                     /locus_tag="P5243_08925"
     CDS             1092..1631
                     /locus_tag="P5243_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRS19615.1"
                     /translation="MESFFKLAQVRRSTRKYKPDVIPVDIITKLQQVLLMSPASKRSN
                     PWEFIFVDDKEILTLLADCKPHGASFVKDAPLAVVIIVDPAKSDVWVEDTSIASIYVQ
                     LAAEDMGLGSCWVQVHKRMHASGEMSSEYIKKILGIPQHFEVESIISIGYKNETRSEF
                     DLEQLQYSKIHTNTYSPKS"
BASE COUNT          601 a          228 c          317 g          601 t
ORIGIN      
        1 tttactccca aaaatactct taatcccgaa tggtttcttt ttaaacatca ggacaaagta
       61 atacattctg tattatatgg agtgttaatg atattattct tatataatta ttcatcgtat
      121 tctcaattgc aaaaacgcgt atttgtatat gcttcagtga gttttatatg tataagtttt
      181 tgtatagaaa tattacagcc aattttgtcg caacgctcgt ttgaatttgc agatattctt
      241 tctaatgcaa taggtttgtt tttaggaatg gcaggatatc gtttgatttt ttacaaaagg
      301 tagaaaaaaa taaatgggtg tacataaatc taattacctt ttataatttt tgaatggggt
      361 atgaggaata ctgaatttta aaaatttagt attgattcat aatgtttact tatgaagata
      421 taggcgaatg gatagtaaaa attttgaaat tgtaaaatta agatgtattt ttataacaaa
      481 taatttggta caatgaattt agatattttc aatattactc acaatcatgt agaaccagct
      541 gttggtaaag tactattgtc ggtgccgttt ataaacgata catattttag tcgttcggta
      601 atattactta cattgcatga ttctgaagga agtgttgggt atattctgaa taaaagaata
      661 ccaatgtttt tacatgaatt ggtagatgat attccacgaa gtaaatttac agtgtgtata
      721 ggtggtcctg ttgcaaacga tacattgcaa atgcttcatt cgcttggaca tatgattcct
      781 gatagtattc aaataaaacc cggattgttt tggggaggta attttgatgc agtaaggagt
      841 ctaataaaaa aacaattaat acaccctcat gaaatacgtt tttttatggg ctattccggt
      901 tggggaaaat tacaattgca cgatgaaata caagcaaatt cttgggtagt taccgatatt
      961 ggtgttgata ctatattaac acatagcgaa gaaaaattat ggaatgctac gttagttaaa
     1021 atgggaagca aatacaaagc atgggcagat tttccgcgtg atccgtcatt aaattaaata
     1081 atacatacaa tatggaatca ttttttaaat tagcacaagt acgtcgtagt acacgtaaat
     1141 ataaaccaga tgtaatacct gtagatatta ttacaaaatt acagcaagtg ttgcttatgt
     1201 cgcctgcaag taagcgtagc aatccgtggg aatttatttt tgttgacgat aaagaaattc
     1261 ttacactatt agccgattgt aaacctcatg gagcttcgtt tgtaaaagat gctcctttgg
     1321 ctgttgtaat tatagtagac cctgcaaaaa gtgatgtgtg ggttgaagat acatctatag
     1381 catcaattta tgtgcaactt gcggccgaag atatggggtt gggaagttgt tgggtacaag
     1441 ttcataagcg aatgcatgca tcgggtgaaa tgtcgtcgga atatataaaa aaaatattgg
     1501 gtatacctca gcattttgaa gttgaatcta ttattagtat tggttacaaa aacgaaactc
     1561 gttccgaatt tgatttagaa caattacaat atagtaaaat acataccaat acatattcac
     1621 cgaaatctta attagaataa aacattatga aacattatgt aattataatt tctcttttga
     1681 taatcgtagc ttgtgttaaa gaacccgatc atcatatacg aattagaaat ttaagtagta
     1741 cacctgt
//
LOCUS       DAOTOW010000532         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53218_length_1745_cov_5.239053, whole genome shotgun sequence.
ACCESSION   DAOTOW010000532 DAOTOW010000000
VERSION     DAOTOW010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53218_length_1745_cov_5.239053"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="P5243_08930"
     CDS             complement(<1..536)
                     /locus_tag="P5243_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS19616.1"
                     /translation="MNNIKFTIIVLLHLCFASNTVVSQIYLSLDSAQSMAIRNNTDIQ
                     TAKQTHTKAELEKQAAYTKYLPKVSAQATGLYIKNNFEKEIYLPTMYPNPATGELYPN
                     IAVNPQTLEPILDDKENPIFNLYSYLPLEISLQGAYMAGLFIEQPIYMGGKIKAGNSM
                     AEKAVLISKDNIALQRSKT"
     gene            complement(538..>1745)
                     /locus_tag="P5243_08935"
     CDS             complement(538..>1745)
                     /locus_tag="P5243_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS19617.1"
                     /translation="QILINTQSNKATIDLAHELQSYIDTLVPEGKIYVKQMQQGQALD
                     APVEVNIYGDNLLHIKMLADTIKTIMKTSKGVGEIHNNFKEDVLGYTIVTNSEAARLG
                     FTTQSIAQLIYLSSNGYSATKMFEGIREVAITLKTQNNKQATVDDLLNLYVQSPITKK
                     NVPLRQIASIQPQWFPGQIIHKNGIRCMTIGCDMAPNYLAADVLKELKPKIETLQMPI
                     GYTYTYGGEQANKTEVFSKMVIALAISLVIIFLILLFQFKNIKETSIIMLTIPFSLFG
                     ALAGLIITNNTFGFTAFMGIISLSGIVVRNAIILIDYIHVLLSEGYDIKTATIEAAKR
                     RMRPIFLTAMAAAIGVVPMIISGSQLWSPLASVIAFGVTWSMITALLTIPVIYYKSLR
                     KQETINYEE"
BASE COUNT          538 a          270 c          279 g          658 t
ORIGIN      
        1 gttttgcttc gttgtaatgc aatattatct tttgatatta gcacagcttt ttcggccata
       61 gaatttcctg cttttatttt acctcccata tatataggtt gttctatgaa caatccggcc
      121 atatacgcac cttgtaaaga tatttccaga ggcaaatatg aatacaaatt aaaaatagga
      181 ttttccttat catcaagtat tggttctaat gtttgcggat ttacggcaat attaggatat
      241 aattcgcctg tggctggatt agggtacatt gtaggcaaat aaatttcttt ttcaaaatta
      301 ttttttatat acaatccggt agcctgagca gaaactttgg gtaaatactt ggtgtaagca
      361 gcttgttttt caagttcagc tttagtatgt gtttgtttgg cagtttgtat gtcagtatta
      421 tttcttatag ccatagattg tgccgaatct aatgaaagat atatttgtga tacaacagta
      481 ttgcttgcaa aacaaagatg taacaatact ataattgtga attttatatt attcatatta
      541 ttcttcataa ttgatagttt cttgttttcg taaactttta taataaatta caggaatggt
      601 gagcaaagct gtaatcatac tccatgtaac cccaaatgca ataacacttg ctagtgggct
      661 ccataattgc gaacctgaaa taatcattgg aactactcca atagctgctg ccattgctgt
      721 caaaaaaatt ggcctcattc ttcgtttagc agcttctata gtagctgttt taatatcata
      781 cccttctgaa agtaatacat gtatatagtc aattagaata atggcattgc gtactacaat
      841 tcccgataaa ctaataattc ccataaaagc cgtaaatcca aaagtgttat tggttataat
      901 taatccggca agagcaccaa ataaactaaa tggtatggta agcataataa tgcttgtttc
      961 ttttatattt ttaaattgga atagtaatat gagaaatatt attactaaac ttatagctaa
     1021 tgcaataacc attttagaaa atacttctgt tttattggct tgttcaccac cgtatgtata
     1081 tgtataacca ataggcatct gtaaagtttc aattttaggt tttagttctt ttaaaacatc
     1141 agctgctaaa taattgggtg ccatatcgca acctatagtc atgcagcgta taccgttttt
     1201 atgaattatt tgtcctggaa accattgtgg ttgtatactt gcaatctgac gcaaaggaac
     1261 atttttttta gttatcggtg attgtacata taaatttaat aaatcatcaa cagtagcttg
     1321 tttattgttt tgtgttttta aagtaatagc aacttctcta atgccttcaa acatttttgt
     1381 tgctgaatat ccgttcgaac tcagatatat aagttgagca atactttgtg ttgtaaagcc
     1441 taaacgagca gcttcagaat tcgtcacaat agtatatcct aatacatctt ctttaaaatt
     1501 attatgaatt tctcctacac ctttacttgt tttcataata gtttttatag tgtcggcaag
     1561 catttttatg tgtaaaagat tatcaccgta tatatttact tctacaggag cgtcaagagc
     1621 ttgtccttgt tgcatttgtt ttacatatat ttttccttcg gggacaaggg tatctatata
     1681 tgattgtaat tcatgtgcta aatcaatagt tgctttgttg ctttgagtat taattagaat
     1741 ttgag
//
LOCUS       DAOTOW010000533         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53308_length_1743_cov_3.369076, whole genome shotgun sequence.
ACCESSION   DAOTOW010000533 DAOTOW010000000
VERSION     DAOTOW010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53308_length_1743_cov_3.369076"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="P5243_08940"
     CDS             <1..473
                     /locus_tag="P5243_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRS19618.1"
                     /translation="YRILYITVVSIILSTYSIAQQTDSLIWHTNFDNAKAIAKETQRP
                     ILLVFSGSDWCKPCILLKKEIFETAEFTAFAKDSLVTVLADFPRQKKNTLSSEQIAIN
                     EGLAKTYNPTGLFPHVVLLSPELHVIRTFGYQKISPRGYIQVLTNAFYSYDTNK"
     gene            457..1290
                     /locus_tag="P5243_08945"
     CDS             457..1290
                     /locus_tag="P5243_08945"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HRS19619.1"
                     /translation="MIQINNFLSKFIPIVSVVSIILIVLYSFSYKAEQVSTPANLSFT
                     FTTTNNGGTYAPNNVLAVWITKTNGDFVKTLIVRGGIRKPKLITWIAQTSGNTIDAVT
                     GPTLTSFGSHTVTWNGTNSAKNIVPDGDYVLHIENVSANQQGPYASFEFVKGNEQYSN
                     TFEDKTYFKNISLQYTPTQVSIERVEKQDANLSISPSRGCVLFQFSNEEFDDVQLTIL
                     SIQGTVVYTVHNPYYLNAMNTIEWHGVDINGHKVPTGTYICKLCIGNKRYTQKFFYSK
                     L"
     gene            1287..>1743
                     /locus_tag="P5243_08950"
     CDS             1287..>1743
                     /locus_tag="P5243_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRS19620.1"
                     /translation="MMRIASIVSILLVLNTIVWAHTQTKILTLMGVRFEITAIASTQQ
                     ETQRGIDVGIAEISRIEQFLSPHIALSEVSIINKNAGVKPVHVSKELCDLILRSKKVS
                     ELSGGLFDVSWASMDPIWRFDGSMKELPAPEIIAHAIRLINYNDIIVNTD"
BASE COUNT          587 a          307 c          302 g          547 t
ORIGIN      
        1 cctatagaat actatatatt acagttgttt caataatact atcaacatat agtatagctc
       61 aacaaacaga ttcattaata tggcatacta atttcgacaa tgccaaagct attgcaaaag
      121 aaacccaacg tcccatttta ttggtttttt ctggttccga ctggtgtaaa ccttgtattt
      181 tattgaaaaa agaaattttt gagactgcag aattcaccgc atttgccaaa gatagtttag
      241 ttactgtatt agctgatttt ccgcgtcaga aaaaaaatac cttgtcgtcc gaacaaattg
      301 ctataaatga aggtttagca aaaacataca atccaacagg tttattccct catgtagtat
      361 tactttcgcc tgaattacac gtaattcgca catttggata tcaaaaaatt agtcctcgcg
      421 gatacattca agtattaact aatgcatttt attcatatga tacaaataaa taatttttta
      481 tcaaaattta tccctattgt ttctgttgta agtattattc tcattgttct gtattcattt
      541 tcatacaaag ctgaacaagt ttcaactcct gcaaatcttt catttacgtt tactactaca
      601 aataatggtg gcacatatgc tcccaataat gtacttgcag tatggattac aaaaactaat
      661 ggagattttg taaaaacgct cattgtaaga ggtggcattc gcaaaccaaa attgattacg
      721 tggatagctc aaacttcggg taatacgatt gatgctgtaa caggacctac gcttacaagt
      781 tttggctccc ataccgtgac atggaacggt accaattctg caaaaaatat tgttcccgat
      841 ggtgattatg tattgcatat agaaaatgta agcgcaaacc aacaaggacc atacgcatcg
      901 tttgaatttg ttaaagggaa cgaacaatac agcaatacgt ttgaagataa aacatacttt
      961 aaaaatataa gtttacaata tacgcctaca caagtttcta tagaacgtgt tgaaaaacaa
     1021 gatgcaaatc tctcaatttc accaagtcgt ggttgtgttt tatttcagtt ttccaatgaa
     1081 gaatttgacg atgtgcaact taccatactt tctattcaag gtactgtggt gtacacggtt
     1141 cacaatcctt attacctcaa tgctatgaat actattgaat ggcatggtgt tgatattaac
     1201 ggacacaaag tgccaacagg aacttatatt tgtaaattgt gcattggaaa taaaaggtat
     1261 acccaaaaat tcttttattc aaaattatga tgcgtatagc atccatagta agtatattgc
     1321 ttgtgcttaa cacgatagta tgggcacata cgcaaacaaa aatacttact ctcatgggag
     1381 ttcgttttga aattacagca atagcctcaa cgcagcaaga aacgcaacga ggaatagatg
     1441 ttggaattgc agaaatatca cgtattgaac aatttttgtc acctcatatt gcactgtcag
     1501 aagttagtat tataaataaa aatgcgggag ttaaaccggt gcatgtaagc aaagaattgt
     1561 gcgaccttat tcttcgcagc aaaaaggtgt cggaactttc gggaggattg ttcgatgttt
     1621 cgtgggcgag catggaccct atttggcgat tcgatggtag tatgaaagaa cttccggcgc
     1681 ctgagattat tgcacatgca attcggctta tcaattacaa tgacattatt gtaaataccg
     1741 ata
//
LOCUS       DAOTOW010000534         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53401_length_1741_cov_3.424674, whole genome shotgun sequence.
ACCESSION   DAOTOW010000534 DAOTOW010000000
VERSION     DAOTOW010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53401_length_1741_cov_3.424674"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="P5243_08955"
     CDS             <1..1470
                     /locus_tag="P5243_08955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS19621.1"
                     /translation="LLLFAFIFNVILYFSFKDKIYLITSGFILSLFLYIFTIDGFSFK
                     YLWNHNTWLASRAAYIIAIISIMLQCYTIQLFYNLHVTYKSWYKVLQVLYVILIVLLG
                     ICWLPYSYFVVSNYVLYGIILIIMMFVTYMSVHIAMQKVQHPKLMMYANCILLCTYIM
                     VLIHNTGIIYLDVIQVFNGKLGVSFLVLFISAAVSERFRNLLMRSKEKLEFEVKLRTI
                     EIEAQKDYLFVQKERILLQNSELNESIRYASKIQQGIFPSIEFFQSLLPKSFIFFKPK
                     DIISGDFYWISVKRGKIIIACADCTGHGIPGAFLSMLGISYLNTIVNEQGITRPDAIL
                     NFLRANIIKTLWKNTEQEPMYDGMNISLCSYDVKLKILEYSGAYNPFYIVRQKSIIKL
                     EPDRMPIGVHIYDKPFSVHKIQLEKKDRVYLFTDGYVDQIGGLDNKKLKRTGFKDLLL
                     SIQGRSIQKQAHKIEQFFNTWKQHNDQTDDVTVIGFEVD"
BASE COUNT          607 a          195 c          287 g          652 t
ORIGIN      
        1 ttgctcttat ttgcctttat attcaatgta attctctatt tttcttttaa agataaaata
       61 tatcttatta cgtcaggatt tatattaagt ctgtttctat atatttttac aatagatgga
      121 ttttcattta aatatttatg gaatcataat acgtggctag cttctcgagc tgcatacatt
      181 atagcaatta tttccattat gttacaatgt tataccatac agttatttta taatttgcat
      241 gttacatata aatcttggta taaagtatta caagtgttat atgtgatttt aattgtactc
      301 ttgggaattt gttggttacc gtattcctat tttgtagtaa gtaattacgt attgtatggt
      361 attatactca tcataatgat gtttgtaaca tatatgagtg tacatatagc aatgcaaaaa
      421 gttcagcatc caaagttaat gatgtatgct aattgtatat tgttgtgtac atatattatg
      481 gttttaatac ataataccgg aataatatat ttagatgtta ttcaggtgtt taatggtaaa
      541 ttaggtgtgt catttttagt tctttttata agtgctgcgg tttcggaacg ttttaggaat
      601 ttgttgatgc gttcaaaaga aaaattagag tttgaagtga aattacgaac aatagaaatt
      661 gaagcacaaa aggattattt atttgtacaa aaggagcgaa ttctgttgca aaattcagaa
      721 ttgaatgaaa gtatacgata tgcttcaaaa atacagcaag gtattttccc atcgatagaa
      781 ttttttcaga gtcttttacc aaaatcattt atattcttta agccaaaaga tattataagt
      841 ggtgattttt attggattag tgtaaaacga ggaaaaataa taatagcttg tgccgattgt
      901 acaggacatg gtattcccgg agcatttttg agtatgctgg gcatatcata tttgaataca
      961 attgtgaatg aacaaggaat aacaagacca gatgctatac ttaatttttt aagagctaat
     1021 ataattaaaa ctttgtggaa aaatacagaa caagaaccta tgtatgatgg catgaatata
     1081 tccttgtgtt catatgatgt aaaattaaaa atacttgaat attcgggagc atacaatccg
     1141 ttttatatag tgagacaaaa gtcaataatt aaattggagc ccgatagaat gccaataggt
     1201 gtgcatatat atgataaacc gttttcggtg cataagatac aactcgaaaa aaaagataga
     1261 gtatatttat ttactgatgg ttatgttgat caaataggag gattagataa taaaaaatta
     1321 aaaagaaccg gattcaaaga tttattacta tcaattcaag gtcgttctat acaaaaacaa
     1381 gctcacaaaa tagaacaatt ttttaataca tggaaacaac acaatgatca aaccgatgat
     1441 gtgactgtaa taggttttga agtagattga tttattgaga gttgtattaa tgttgaaaaa
     1501 ttcaacttag cttattaaca atacttagtt tgattttttt acagttgctt attaaagcat
     1561 gaaattgcat tttgaaaatt cttatatttt tagtttattt gtattcttgt gagcgatttt
     1621 aattatgagg atatatttac tttgggttgc aatagttgtt ttaggaattt cttgttcaag
     1681 taattctgtt atgacgcaaa ccaatcattc atcaaataat tttttcgata ttactgcaat
     1741 t
//
LOCUS       DAOTOW010000535         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53699_length_1735_cov_3.701190, whole genome shotgun sequence.
ACCESSION   DAOTOW010000535 DAOTOW010000000
VERSION     DAOTOW010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53699_length_1735_cov_3.701190"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="P5243_08960"
     CDS             <1..959
                     /locus_tag="P5243_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19622.1"
                     /translation="SSLLGLLFMIVFTPITMYLAFQQNITGKELVHDCGCFGDALILT
                     NWQTFVKNLIILIPITIVFLYRKRVEDVLNHSRSLIVTSVFTLAVVGLSIYALLHLPP
                     IDFRPYKKGTKLVNAACSETGLQSDTKTYMYAQFKNLQTGEKKEFEITENYPDYNVWQ
                     YDTTVAIRYEKVALQQESDSIQKPKQRIFAVDNMLFAKDGEDYTCEIIGDTSYVFLVV
                     QYDVEHTNTKPQQTLNELFTWANKQGFTFYAATSSLNEKVEAYKEKYNVQYTFLDADD
                     IPLKTIVRANPGLVLLKDGVVINNWHGNDIPSIEEFSKLLIK"
     gene            982..1731
                     /gene="tpiA"
                     /locus_tag="P5243_08965"
     CDS             982..1731
                     /gene="tpiA"
                     /locus_tag="P5243_08965"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRS19623.1"
                     /translation="MRKKVVAGNWKCNTTVAEGVALAKEVNDIVTATDVVVVLGTPFT
                     HITEVVKTITNKAISVAAQNCAAEPKGAFTGEVSAAMVQSTGAKYVILGHSERREYYG
                     ETSEILNKKIALALANGLTPIYCCGEALPVRESGKQNEFVINQLQETIFTLSPDDFKK
                     IIIAYEPIWAIGTGKTATTQQAQDMHKAIRDAITAKYGNDIAQETSILYGGSCNAQNA
                     KELFANPDVDGGLIGGASLKAADFNTIIMSY"
BASE COUNT          651 a          285 c          316 g          483 t
ORIGIN      
        1 actcgtcgtt gctcggatta ttatttatga ttgtatttac gccaattacc atgtatttgg
       61 cgtttcagca aaatattacc ggtaaagaat tggttcatga ttgtggttgc tttggcgatg
      121 cactcattct taccaactgg caaacatttg taaaaaatct tatcattctt ataccaatta
      181 ccattgtttt tttgtatcgc aagcgggtag aagatgtttt gaatcattcg cgttcactta
      241 tagtaacatc ggtttttaca cttgcagtag taggactttc aatatatgca ttattgcact
      301 tacctcctat tgactttaga ccatacaaaa aaggaacaaa attggtaaat gcggcgtgtt
      361 cagaaaccgg attacaatct gatacaaaaa cctatatgta tgctcagttc aaaaatttgc
      421 aaaccggcga gaaaaaagag tttgaaataa ccgaaaatta tcccgattac aatgtgtggc
      481 aatatgatac aaccgtagca atacgatacg aaaaagtagc attacaacaa gaatctgatt
      541 ctatacagaa acccaaacaa cgcatatttg ctgttgacaa tatgctgttt gccaaagatg
      601 gtgaagatta tacgtgcgaa ataattggcg atacatcata tgtattttta gtagtgcaat
      661 acgatgtaga acacaccaat acaaaaccac agcaaacact aaatgaattg tttacatggg
      721 caaacaaaca aggatttact ttttatgcag caacatcgag cttaaacgaa aaagtagaag
      781 catataaaga aaaatataat gtacaatata cgtttttaga tgccgacgat ataccgctaa
      841 aaacaatagt gcgagccaat ccgggtttgg tgctgttaaa agatggtgta gttataaaca
      901 attggcatgg aaatgatata ccaagtattg aagaattttc gaaattactt attaaataat
      961 atattaaatt tttaaataca aatgagaaaa aaagtagtag ccggtaactg gaaatgtaat
     1021 acaacagttg ccgaaggtgt agcattagca aaagaagtaa atgacatagt tacggcaacc
     1081 gatgtagttg tagtattagg aactccattt acacacatta ccgaagtagt aaaaacaata
     1141 acaaacaaag caatttctgt agcagcacaa aactgtgcag ccgaacctaa aggagctttt
     1201 acaggtgaag tatcggcagc aatggttcaa tcaacaggag caaaatatgt tattttagga
     1261 cacagcgaac gaagagaata ctatggtgaa acaagcgaaa ttttgaataa aaaaatagca
     1321 ttggcattag caaacggact tacaccaatt tattgttgtg gcgaagcatt acctgttcgc
     1381 gaatcgggta aacaaaacga atttgtaatc aaccaattac aagaaactat ttttacgtta
     1441 tcacccgatg atttcaaaaa aataataata gcatacgaac ctatatgggc aattggtacc
     1501 ggaaaaacag caacaactca acaagctcaa gatatgcaca aagcaatacg cgatgctata
     1561 actgcaaaat atggcaatga tatagctcaa gaaacttcaa ttctatatgg tggaagttgt
     1621 aatgcacaaa acgcaaaaga attatttgca aatcctgatg ttgatggtgg tttaataggt
     1681 ggagcttcgt taaaagctgc cgatttcaac actattatta tgtcatatta atgag
//
LOCUS       DAOTOW010000536         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53712_length_1735_cov_2.839881, whole genome shotgun sequence.
ACCESSION   DAOTOW010000536 DAOTOW010000000
VERSION     DAOTOW010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53712_length_1735_cov_2.839881"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="P5243_08970"
     CDS             <1..677
                     /locus_tag="P5243_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRS19624.1"
                     /translation="DNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGFLQERITST
                     KRGSITSVQAIYVPADDLTDPAPATAFAHLDATTVLSRKIVELGIYPAVDPLESTSRI
                     LTPEIVGEAHYNTAQRVIQILQRNKELQDIIAILGMEELSEEDKLVVHRARRVQRFLS
                     QPFHVAEQFTGMPGKLVSIEDTIKGFNMIIDGEVDKYPEAAFNLVGTIEEAIEKGEKM
                     LANITK"
     gene            683..919
                     /locus_tag="P5243_08975"
     CDS             683..919
                     /locus_tag="P5243_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRS19625.1"
                     /translation="MEIEIITPSTQLYNGKASQVNVPGSNGSFEILENHAPIISTLQK
                     GTIILIDQTGTKHSFDIPGGVVEQSKNKVLILVD"
     gene            1018..>1735
                     /locus_tag="P5243_08980"
     CDS             1018..>1735
                     /locus_tag="P5243_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRS19626.1"
                     /translation="MRILTYICILILCIVTKHTQAQRTVTFFASDGLEITADIYDTDV
                     ESSKYMLLFHQAEYSRGEFQQIATRLIKLGFTCIAVDLRYGNEVNFVNNETAMRAKKG
                     GFPQSMLDCEKDILATISYVKSIDTKPRIFLLGSSFSASLCLKIANERTDIEAVIGFS
                     PGEFFGNDLRVEEAIRGLQTPYFVGCPRSEHYYVLQLLSGTQSKKKIIFKPEGSDGLH
                     GAKTLWWESATRDEFWLSLLF"
BASE COUNT          611 a          291 c          291 g          542 t
ORIGIN      
        1 tagataatat tttccgtttt acgcaagcag gttcagaagt atcagctctt ttaggacgta
       61 tgccttcagc cgtaggatat caaccaacac ttgctactga aatgggattt ttacaagagc
      121 gtattacatc tactaaacga ggttctatta cttcggtaca agctatatat gtacctgccg
      181 atgacttaac agaccctgct cctgctacgg catttgccca tcttgatgct acaactgtat
      241 taagtcgtaa aattgtagaa cttggtattt accctgctgt tgatcctctt gaatctacgt
      301 cacgtatttt aacacccgaa attgttggtg aagctcatta taatactgca caacgagtaa
      361 ttcaaattct gcaacgcaac aaagaattac aagatattat agctattctt ggtatggaag
      421 aattatctga agaagataaa ttagttgttc atagagcacg acgtgttcaa cgatttttat
      481 cacaaccatt tcatgttgcc gaacaattta ccggtatgcc gggtaaatta gtttctatag
      541 aagataccat caaaggtttt aacatgataa ttgacggaga agtagacaaa tatcctgaag
      601 ctgctttcaa tttagttgga actatagaag aagctataga aaaaggtgaa aaaatgcttg
      661 ctaacattac taaataaacc gtatggaaat agaaattata actccatcaa cgcaactata
      721 caacggtaaa gcatctcaag taaatgttcc cggtagcaac ggttcgtttg aaatactgga
      781 aaatcatgct cctattattt ctacactaca aaaaggaact attatattaa ttgatcaaac
      841 cggaacaaaa cattcctttg atatacctgg aggagttgta gaacaatcta agaataaagt
      901 attaatatta gtagattaaa aatattccaa aaacattaaa aagtcactat acaaatagtg
      961 actttttaat tataatttgt aactactata tatattggtt cgtaccaata caaaactatg
     1021 agaatattaa cctacatatg tatacttata ttgtgtattg ttactaaaca tacacaagct
     1081 caaagaacag ttaccttttt tgcgtctgac ggattagaaa taactgctga catatatgat
     1141 acagatgttg aatcatctaa atatatgtta ctttttcatc aagcagaata tagtagaggc
     1201 gaatttcaac aaatagcaac aagacttatt aaacttggat ttacttgcat tgctgtagat
     1261 ttacgatatg gtaatgaagt aaattttgta aataacgaaa cagcaatgcg tgctaaaaaa
     1321 ggtggttttc cacaatcaat gcttgattgc gaaaaagaca tactagcaac aatatcatac
     1381 gtaaaatcta tcgacacaaa accacgtatc tttcttttag gaagttcgtt ttctgcttca
     1441 ttatgcttaa aaattgctaa cgaacgaaca gacattgaag ctgttatagg atttagtcct
     1501 ggagaatttt ttggaaatga tttacgtgtt gaagaagcaa tacgtggact acaaactcca
     1561 tattttgtcg gatgtcctcg aagcgaacat tattatgtac ttcaattact ttcgggaact
     1621 caatcaaaaa agaaaataat ttttaaaccc gaaggctctg atggtttaca tggagcaaaa
     1681 acactctggt gggaatctgc aactcgcgat gaattttggt taagtttgtt atttt
//
LOCUS       DAOTOW010000537         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_53855_length_1732_cov_2.191413, whole genome shotgun sequence.
ACCESSION   DAOTOW010000537 DAOTOW010000000
VERSION     DAOTOW010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53855_length_1732_cov_2.191413"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="P5243_08985"
     CDS             complement(<1..586)
                     /locus_tag="P5243_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19627.1"
                     /translation="MKKFGFLFAVMFNCLTGGIIAMLLGVAPVMGAVAMNAVGAFAPK
                     GEKGVLMAGIYAEVWTGEMIKAFRNSMESLGWINRIRSYDQYAQNDVIHFVNLGGDPT
                     VLVNNTTYPIGIENLNDADKAISLDKFQTKATRVTDDELYAITYDKMGSVIERHRESI
                     DQKKYAKALHALAPSSHAAKTPVLLTTGVTASDGT"
     gene            complement(607..1557)
                     /locus_tag="P5243_08990"
     CDS             complement(607..1557)
                     /locus_tag="P5243_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HRS19628.1"
                     /translation="MSKVVISNSRVNSYGSRVLTEGIDLTQYKRNPILLWMHNRPWRG
                     TKDEVLPLGRVEDIIVDGDNLLGTVVFDETDEFSRSIKAKWDAGTLKMVSPGLDVVEM
                     SDSAEYILPGQRRATVTKSKLVEVSIVDLGANDDALALYKDGKIINLSEGNEHFIPEL
                     VCNLNIDNNMKTIALKLGLPEVATEQEILQKISELQGTAGRVGELELAVQEQKNNAIV
                     LAVEAAIQAKKITADKKEHFVTLGKTAGIELLNKTLDSIQPASKPTDFIGSGGDSKKV
                     EYKKLSDVPTDKLEAMRSEDKEQYMALFKAEYGYEPKLYV"
BASE COUNT          517 a          395 c          240 g          580 t
ORIGIN      
        1 gtgtgccatc agaagccgta actccggttg ttaacaacac tggtgttttt gcggcatgcg
       61 atgacggtgc aagtgcatgc aaagctttcg catatttttt ctgatctata ctctctctat
      121 gtcgctctat tacactaccc attttgtcgt atgttatagc atacaactca tcatctgtta
      181 cccgtgtagc ttttgtctga aatttatcaa gcgatatagc cttgtctgca tcatttaaat
      241 tttcaatacc aatagggtag gttgtgttgt ttactaatac agtcgggtct ccacctaaat
      301 taacaaaatg tatcacgtcg ttttgtgcat attgatcata cgaacgtatt ctgttaatcc
      361 aaccaagaga ctccattgag tttctaaatg ctttaatcat ttcgccggtc catacttctg
      421 cataaatacc tgccattaaa acaccttttt ctcctttagg agcaaatgct ccaacagcat
      481 tcatagctac tgcacccatc acaggagcaa caccaagaag catagcgatt ataccgcctg
      541 tcaaacagtt gaacatcacg gcaaataaaa atccaaattt tttcatcttt ttttctatta
      601 ttttagttat acatacaatt ttggttcata cccgtattca gctttaaata aagccatata
      661 ctgttctttg tcttcagaac gcatagcttc caacttatca gtcggaacat cgctcagttt
      721 tttgtactca acctttttac tatcaccacc acttccaata aagtcagttg gcttagaagc
      781 aggctgtatc gaatcaagag ttttattcaa caattctata cctgcagttt ttccaagagt
      841 aacaaaatgc tcttttttat ctgctgtaat ttttttcgcc tgtatagcag cttctacagc
      901 caatacaata gcgttatttt tttgctcctg cacagcaagt tcaagttcgc caactctacc
      961 tgcagtgcct tgcaattcag aaattttctg caaaatttct tgttcggttg ccacttccgg
     1021 caaacctaat tttaatgcaa tagttttcat attattatca atgtttaagt tacatactaa
     1081 ttcaggaata aaatgttcgt ttccttccga taggttaatt atcttaccat ctttatatag
     1141 agcaagtgca tcgtcattag cacccaagtc aacaatactt acctctacaa gttttgattt
     1201 cgtaactgta gctctacgct gccctggcaa tatgtattca gcagaatcac tcatttccac
     1261 aacatcaagt ccgggactta ccattttaag agttccggca tcccattttg cttttatcga
     1321 tctactaaac tcatcagtct catcaaatac aacagtacct aacaaattgt cgccatctac
     1381 tattatatct tcaaccctgc caagcggcaa tacttcatct ttagtaccac gccatggtct
     1441 attgtgcatc cacaacaaaa taggattgcg tttgtactgc gtcaaatcta taccttcagt
     1501 aagcactcgc gaaccataac tatttactcg tgaatttgaa attacaactt tgctcatttt
     1561 tatttcattt attaaatcac agcaaaacta tctacacgcg tagcaaaaaa aaaataaaag
     1621 tcgtaccata attttctata gtacaagcat taaccaagtt ttttacccta cccgaaaata
     1681 caagtatttt cgcctaaaaa gcattaatat gaataataca aacaaatctg aa
//
LOCUS       DAOTOW010000538         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54124_length_1725_cov_3.231737, whole genome shotgun sequence.
ACCESSION   DAOTOW010000538 DAOTOW010000000
VERSION     DAOTOW010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54124_length_1725_cov_3.231737"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1725
                     /locus_tag="P5243_08995"
     CDS             <1..>1725
                     /locus_tag="P5243_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRS19629.1"
                     /translation="QDVGELKNEQELQEEAETHTYIYDLNLQQLVDEINKYNAKDAVL
                     KHRTRIELIRAEFYKKLKQDSENAKNLFIQNGGEPKNFVYSNPIEQDFKHVYSSIKEF
                     KAELLQEAEREKQLNLQKRLAIIEELKELVTKQESSGKTFNEFKSIQERWRAIGAIPQ
                     SEIKSIWEQYHHHVGVFYDYIKINKALRDLDLKKNLEAKMQLCEKAEALLLEPQIVKA
                     FAQLQKLHDLWREVGPVADEKKDEIWNRFKEATNKLNKAHQEYFAQLKEQEKTNYELK
                     SGLCERVEHMLEQEFTTLKQWNEASEEISAIQKTWKTIGFAPQKYNTAVYERFCKACD
                     DFFAKKREFFLTVKEEEDVNKQKRIDICVQAEQMKTRTDWKQATQDFLDLQQAWKEIG
                     PVSHKESTKLWNRFRAACDEFFNARQQFYKDKESEQQTNYQLKLAVIEKIKNIQTDLK
                     PQDKLLALQALQKEWTEIGFVPAKKKDALQKQYKEAVQKQFDAIQLPTEQKQKALLEV
                     KVHTMLTDSKGDDMIHAEIAKLQNRIRVIEGEISVLENNIGFFSKSKNSEKIIEDFNK
                     KVAKGKEE"
BASE COUNT          742 a          210 c          316 g          457 t
ORIGIN      
        1 ctcaagatgt tggagaattg aaaaatgaac aagaattgca agaggaggca gaaactcata
       61 catatatata tgatttgaat ttgcaacaat tagttgatga aattaacaaa tataatgcta
      121 aggatgcagt tttgaagcat agaacacgta tagagcttat acgtgctgaa ttttataaaa
      181 aattaaaaca agattcagaa aatgccaaaa atctttttat acaaaatggt ggagaaccaa
      241 agaattttgt gtattcaaat ccaattgaac aagattttaa acatgtgtat tcttcaataa
      301 aagaatttaa agcagaatta ttacaagaag ctgaacgtga aaaacaatta aatctacaaa
      361 aacgtcttgc gataattgaa gaattaaaag aattagttac caaacaagaa tcgtcaggaa
      421 aaacatttaa tgaatttaaa agtattcaag aacgttggcg agctatagga gctattccac
      481 aatccgaaat aaagtcgatt tgggaacaat atcatcatca tgtaggagtg ttttatgatt
      541 atattaaaat taataaggca ctgagggatt tagatttaaa gaaaaattta gaagcaaaaa
      601 tgcaattgtg cgaaaaggca gaggcattgt tgcttgaacc acaaatagta aaggcgtttg
      661 ctcaattaca aaaattacat gatttgtgga gagaagttgg cccggtagcc gatgaaaaaa
      721 aagatgaaat ttggaataga tttaaagaag caacaaataa attaaataaa gctcatcaag
      781 aatattttgc tcaacttaaa gaacaagaaa aaactaatta cgaattaaaa tcaggattgt
      841 gtgaaagagt tgaacacatg cttgagcaag aatttacaac attaaaacaa tggaatgaag
      901 cttctgagga aatttctgca attcaaaaaa cgtggaaaac aataggattt gctcctcaaa
      961 aatataatac tgccgtatat gaacgatttt gtaaagcatg cgatgatttt tttgctaaaa
     1021 aacgagagtt ttttcttacg gtaaaagaag aagaagatgt aaacaagcaa aaacgtattg
     1081 atatatgtgt gcaggcagaa caaatgaaaa caagaaccga ttggaaacaa gcaacacaag
     1141 attttttgga tttacaacaa gcttggaaag aaattggtcc tgtttcgcat aaagaatcta
     1201 caaaattgtg gaatagattt agagctgcat gtgatgagtt ttttaatgca aggcaacaat
     1261 tttataaaga taaggaaagc gagcaacaga caaattatca attgaagctt gctgtaattg
     1321 aaaaaattaa aaatatacaa acagatttaa agcctcaaga taaattgctt gcattacaag
     1381 cattgcaaaa agaatggacg gaaataggat ttgtgcctgc aaaaaagaaa gatgcattgc
     1441 aaaaacaata taaagaagcc gtgcaaaaac aatttgatgc cattcaatta cctaccgaac
     1501 aaaaacagaa agcattgctt gaagtaaaag tgcatactat gcttaccgat tcaaaaggtg
     1561 atgatatgat tcatgcagaa atcgccaaat tacaaaatag aattcgtgtt atagaaggtg
     1621 aaattagtgt gcttgaaaat aatataggtt tcttttcgaa aagtaaaaat tcagagaaaa
     1681 ttattgaaga ttttaataaa aaggtagcaa aaggtaaaga agaaa
//
LOCUS       DAOTOW010000539         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54174_length_1724_cov_2.349910, whole genome shotgun sequence.
ACCESSION   DAOTOW010000539 DAOTOW010000000
VERSION     DAOTOW010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54174_length_1724_cov_2.349910"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="aepY"
                     /locus_tag="P5243_09000"
     CDS             complement(<1..1091)
                     /gene="aepY"
                     /locus_tag="P5243_09000"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HRS19630.1"
                     /translation="MIKVQDFYNELLKNDIAFFAGVPDSLLKDICAYITSHTPREKNI
                     ITANEGGAIALGAGYYLATNKIPLIYMQNSGIGNAVNPLLSLADKEVYSIPMILLIGW
                     RGEPQTKDEPQHVKQGRVTPKLLKSMEIPFQIMDGKSNYEKIINYTVKKAKEILAPVA
                     ILVRKNTFDSFLLNPCENNIYELTREKALEEVLQFIPKNSVIVTSTGMLSRELFELRE
                     KHKQSHKTDFLTVGSMGHTSQIALGIALGRPEKQVFCFDGDGSAIMHLGNYAITSNIS
                     PNNLKIIIFNNGAHDSVGGQPTVGQWIDFSKIADAMHMKDFGSIQTQQEIKGILPQFI
                     NYPTIAFLDIRIKKGARKDLGRPTSTPLQ"
     gene            complement(1103..>1724)
                     /locus_tag="P5243_09005"
     CDS             complement(1103..>1724)
                     /locus_tag="P5243_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRS19631.1"
                     /translation="PIVYDGDTGGIPEHFVFTVRTLERLGISAIIIEDKVGLKKNSLF
                     GNEVKQQQASVDEFCYKIEAGKKTQITNDFMIIARIESLICGKDTDDAIMRAKSYVEA
                     GADGIMIHSKEKTGFDIKEFCTKFREINTTTPIVVVPTTYNHINEEILESWGVNIVIY
                     ANHMLRAAYPAMVKAAESILLNKRSLEADSICMPINEILSLIPGTK"
BASE COUNT          507 a          305 c          255 g          657 t
ORIGIN      
        1 ttttgcaatg gagtagatgt tggccgtcct aaatcttttc gtgctccttt ttttatgcga
       61 atatctagaa aggcgattgt ggggtaatta ataaattgag gaagtatacc tttaatttct
      121 tgttgggtct gtattgaacc aaaatctttc atatgcatgg catcagcaat tttcgagaaa
      181 tcaatccatt gcccaacagt aggttgacca cctacagaat cgtgtgcacc attattgaat
      241 attattattt tcaggttgtt tggagatata ttactagtaa ttgcataatt acccaaatgc
      301 ataatagctg aaccatcacc atcaaaacaa aaaacttgct tttcaggtct tcccaaagct
      361 atccctaatg caatttgtga tgtatgcccc atagatccaa cagtaagaaa atctgtctta
      421 tgtgattgtt tgtgtttttc acgtaattca aaaagttcac gagataacat tccagtacta
      481 gtaacaatta cagaattttt tgggataaat tgtaaaactt cttctagtgc tttttcacga
      541 gtaagttcat aaatattatt ttcacatgga tttaataaaa aggaatcaaa tgtatttttc
      601 cttacaagaa tagctactgg tgctaatatt tcttttgctt tctttactgt gtaattaata
      661 attttttcat aattggattt cccatccata atttgaaaag gtatttccat gctttttaac
      721 aatttgggcg ttactctacc ttgttttaca tgttgtggtt catcttttgt ttggggttca
      781 cctctccaac cgattaataa aatcattgga attgaataaa cttctttgtc tgctaaagat
      841 aggagaggat taactgcgtt tcctattccg gaattttgca tatatattaa gggaatttta
      901 tttgttgcta agtaataacc ggctcccaaa gcaatggctc caccttcatt ggctgttata
      961 atattctttt ctcttggtgt atgagatgtt atatatgcac atatatcttt tagtaaagaa
     1021 tccggcacac cagcaaaaaa agcaatatca tttttaagca attcattata aaaatcctgt
     1081 acctttatca tttctaaatt ttttattttg ttcctggaat tagagataaa atttcattta
     1141 taggcataca tattgaatcc gcttctaaac tacgtttatt taataaaatt gattctgctg
     1201 cttttaccat tgccggatac gctgcccgta acatatgatt tgcataaata actatattaa
     1261 cgccccaact ttctaatatc tcttcattga tatgattata agttgtaggt actaccacaa
     1321 taggagttgt tgtattgatc tctctgaatt ttgtgcaaaa ttcttttatg tcaaagcctg
     1381 ttttttcttt gctgtgtatc attattccgt ctgcgcctgc ttctacataa ctttttgccc
     1441 tcataatagc atcatcagta tcttttccgc aaattaggct ttcaatacgt gcaatgatca
     1501 taaaatcatt ggtaatttga gtttttttac ccgcttcaat tttataacaa aattcatcta
     1561 ctgaagcttg ttgttgtttt acctcatttc caaataatga attctttttt aaaccgactt
     1621 tatcttcaat tattattgcg gagattccaa gcctttccaa agttctaact gtaaaaacaa
     1681 aatgttcagg tatgcctcct gtatctccgt catatactat cggt
//
LOCUS       DAOTOW010000540         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54305_length_1721_cov_2.973589, whole genome shotgun sequence.
ACCESSION   DAOTOW010000540 DAOTOW010000000
VERSION     DAOTOW010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54305_length_1721_cov_2.973589"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..552
                     /locus_tag="P5243_09010"
     CDS             97..552
                     /locus_tag="P5243_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HRS19632.1"
                     /translation="MGIFNTIKKNILSDIKVELTEEFDRNFERKGFFSNRWSDRKRQN
                     IGSLMQATGTLRKSIRSKVQGNSVMFLSSVPYASLHNSGGEITQSVRPHTRQRKIDKK
                     KYLVRAHTRKINMPQRQFIGNSHQVQSIVKNIVNENLQQIGTYITKKYK"
     gene            554..1060
                     /locus_tag="P5243_09015"
     CDS             554..1060
                     /locus_tag="P5243_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19633.1"
                     /translation="MRDLLYKEIVARLKAITIVDNEYIINTENTSPAIQHFGIWNEQL
                     QNIEAEIPFYTPAIFIEFMPINWTALGNNVKDSVVQIVLHTLTDIDYSTSTDTIDAFA
                     IENAIQLCLEGFKSDNIGGFTLEESITDTNFAEIMHNKERYAVYIKNNTATLKRITID
                     KPTIQINV"
BASE COUNT          643 a          296 c          251 g          531 t
ORIGIN      
        1 aaaggcaaca accgaagcta ttaccttttt tgggaggtta agggcgaacc ataaccattg
       61 caaacataca aaatttttat attaaaagtc aaacaaatgg gaatatttaa tactataaaa
      121 aaaaatatac ttagcgatat taaggtagaa ttaaccgaag agtttgacag aaacttcgaa
      181 cgaaagggtt ttttttcaaa ccgatggagc gaccgtaaac gacaaaacat tggttcacta
      241 atgcaagcta ccggaacact tcgcaaatct attcgttcca aggtacaggg caattctgtt
      301 atgttcttaa gcagcgttcc ttacgcttcg ttacacaata gcggtggcga aattacacag
      361 agtgtgcgac ctcatactcg gcaacgtaaa atagataaaa aaaagtacct ggtgcgtgca
      421 cacactcgaa aaataaatat gccacaacgc caatttattg gtaatagcca tcaggtgcaa
      481 agcattgtaa aaaacatagt caacgaaaat ctacaacaaa ttggtaccta cataactaaa
      541 aaatataaat aatatgagag atttattgta caaagaaatt gttgctcggc tcaaagcaat
      601 tacaattgta gacaacgagt atattataaa tacagaaaac acaagccctg ctattcagca
      661 ctttggaata tggaatgagc agcttcagaa catagaagca gaaataccat tttatacgcc
      721 ggcaattttt attgaattta tgcctattaa ttggacagca ttgggaaaca atgttaaaga
      781 ttcggtagtg caaatagtat tgcatacgct cacagatatt gactatagca catctaccga
      841 cactatagat gcctttgcta ttgagaatgc aatacaattg tgtttagaag gatttaagtc
      901 tgataatatt ggaggtttta cactcgaaga atcaattact gacacaaatt ttgctgaaat
      961 catgcacaat aaagaacgct atgctgttta cataaaaaac aacacagcaa cacttaaacg
     1021 tataactatt gacaaaccta caatacaaat caatgtctaa aaaaaagcgt gaagctccta
     1081 cacttcacgc aaaaacttat actattttac acgaactaaa aatctaaaga cttttgaatg
     1141 tgttggataa attgtttttc cattctttcg aatatattta caatatatca ccttgtatac
     1201 atttttgttg acattttcag acatacagct atgccacctc ctttcttttt tgatgttacc
     1261 atctaaaaat actttcaagt cctaccttga aagcaaaaaa cccagtagta ggatactggg
     1321 ttaatttttt ttgaggattt ttttgttagt tttttgaggt ggctaacaat tatgtcgtca
     1381 aatgtcatta caaaaatact tattttttaa aggattccaa tttttttgta actattttac
     1441 aaatatttct aaaataattg taaaaacact tgacttgttg ttgtttttaa catagaaaaa
     1501 aaaagcccaa tactctaata tcgggctttc tatgcaatta taaacaatct tacttattgt
     1561 ttttttggaa tattgccaaa ttttaaaaat gcatttacac aaaaaataat tacagcacct
     1621 ccaaaaattg aaccctctat tattttatct tttataatta acaaatatga taaatacatg
     1681 cccataccta ttacaattaa agctgctaca attttgaaaa c
//
LOCUS       DAOTOW010000541         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54485_length_1717_cov_3.157040, whole genome shotgun sequence.
ACCESSION   DAOTOW010000541 DAOTOW010000000
VERSION     DAOTOW010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54485_length_1717_cov_3.157040"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1717
                     /gene="pnp"
                     /locus_tag="P5243_09020"
     CDS             <1..>1717
                     /gene="pnp"
                     /locus_tag="P5243_09020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRS19634.1"
                     /translation="YHAEVFVNVMLMSADSIDMPDALAGLAASAALAVSDIPFNGPIS
                     EVRVARVNGSFVINPTFQQLETADIDIMVAATIDNIMMVEGEMKEVSEAEMLEAIKVA
                     HEAIKVHCKAQMELTEMVGKTKKREYCHEEHDEELKQDIWAKTYDKVYAVATSCNADK
                     HARAQAFNTIKDEYIASLPAEDAEAKAFLIGQYYHAVEKEAMRRAILDEGKRLDGRKT
                     TEIRPIWSEIDYLPMAHGSAVFTRGETQSITTVTLGMKNDEKLVDEVLYKGKERFLLH
                     YNFPPFSTGEAKASRGVGRREIGHGNLAFRALKAVIPSDYPYVIRIVSDILESNGSSS
                     MATVCAGTLALMDAGVAITKPVSGIAMGLISENKGSKFAVLSDILGDEDHLGDMDFKV
                     TGTKDGITATQMDIKVDGLSYEILEKALNQAKEGRMHILGKITETIAEPRADLKPHAP
                     RIERLIIDKEYIGAIIGSGGKNIQELQARTNTIIAIEEINGKGEVEICGTDAQGMSEA
                     KAYIMGIAQEPEIGKVYSAKVKTIVEFGAFMEFLPQKDGLLHVSEIDWKRIEKVGDVL
                     KEGDV"
BASE COUNT          584 a          235 c          373 g          525 t
ORIGIN      
        1 attatcatgc agaggtgttt gtaaatgtaa tgttaatgtc tgccgatagt atagatatgc
       61 ctgatgcttt agcaggtttg gctgcttctg cagctttagc tgtttctgat atacctttca
      121 atggtccaat ttcagaagtt agagttgctc gtgtgaatgg ctcttttgta atcaatccta
      181 cttttcaaca attagaaacg gcagatattg atataatggt tgctgctact attgataata
      241 taatgatggt agagggtgaa atgaaagaag tttcagaagc tgaaatgctt gaagctataa
      301 aggttgcaca tgaagctata aaagttcatt gtaaagctca aatggaatta accgaaatgg
      361 ttggtaaaac aaaaaaacgt gaatattgtc atgaagagca tgatgaagaa ttgaaacaag
      421 atatttgggc taaaacatat gataaagtat atgcggttgc tacgtcatgt aatgccgata
      481 aacatgctcg agctcaagct tttaatacaa taaaagatga atatattgca tcgttacctg
      541 cagaagatgc tgaagctaaa gcttttttaa ttgggcaata ttatcatgct gtggaaaaag
      601 aagctatgcg tagagctata cttgatgaag gcaaacgatt agatggtaga aaaactacag
      661 aaattcgtcc aatttggtca gaaattgatt atttacctat ggctcatggt tcggctgtgt
      721 ttactcgtgg tgaaacacaa tcaattacta ccgtaactct tggtatgaaa aacgatgaga
      781 aattggtaga tgaggtatta tacaaaggta aagaaagatt tttgttacat tataattttc
      841 ctccattttc aacaggcgaa gcaaaagcat caagaggtgt tggccgtcgt gaaattggac
      901 atggtaattt agcatttaga gctcttaaag ctgttatacc ttctgattat ccatatgtaa
      961 ttcgtattgt atctgatatt ttagaatcta atggttcttc ttctatggct acagtgtgtg
     1021 ccggaactct tgctctaatg gatgcaggtg ttgcaattac gaaacctgtt tcgggtatag
     1081 caatggggct aatttcagaa aataaaggtt ctaaatttgc tgttctttct gatattcttg
     1141 gcgatgaaga ccacttagga gatatggatt ttaaagttac aggaaccaaa gatggtatta
     1201 ccgctacgca aatggatata aaagttgacg gattatcata cgaaattctt gaaaaagcac
     1261 taaatcaagc taaagaggga cgtatgcata tacttggtaa aataactgaa actatagctg
     1321 aacctcgcgc cgatttaaaa cctcatgctc ctcgtattga acgattgata atagataaag
     1381 aatatatagg agctataata ggttcaggcg gaaaaaatat tcaagaatta caagcaagaa
     1441 ctaatactat aattgctatt gaagaaataa atggtaaagg tgaagtagaa atttgcggaa
     1501 ccgatgcgca gggtatgagt gaagcaaaag cttatataat gggtattgct caagaacctg
     1561 aaataggtaa agtatattct gcaaaggtta aaactattgt agagtttggt gcatttatgg
     1621 aatttttgcc tcaaaaagat ggtctgttac atgtgtcaga aattgactgg aaacgaatag
     1681 agaaggttgg agatgttctt aaagaaggtg atgtgtt
//
LOCUS       DAOTOW010000542         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54526_length_1716_cov_2.865744, whole genome shotgun sequence.
ACCESSION   DAOTOW010000542 DAOTOW010000000
VERSION     DAOTOW010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54526_length_1716_cov_2.865744"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="P5243_09025"
     CDS             <1..772
                     /locus_tag="P5243_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HRS19635.1"
                     /translation="NVIENPLIDEVEAMLPGANCGGCGFPGCRKFADVLVNSESIEGL
                     FCSPAGQEQMEKIAEHLGKSVSKKAMQVAVVRCNGSCDVRPVTNEYEGISSCAFAAQL
                     YAGETACSFGCLGLGDCELVCAFDAIHVNVITGLPEVITDKCTACGACVDACPKQIIE
                     LRKTNKKDLKIYVSCVNKDKGVVAKKACDVACIGCGKCVKVCPHSAITLQNSLAYIDD
                     ELCKLCRKCVDECPTHAIIETNFPEKKKQVQENSSDIE"
     gene            817..>1716
                     /locus_tag="P5243_09030"
     CDS             817..>1716
                     /locus_tag="P5243_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HRS19636.1"
                     /translation="MKLRKKYAMQTFTKGGIHPLEHKLSSKQPIEMLPIPEFVQISLS
                     QHIGVAATPIVKVGDSVKVGTCIGESAGFISARVHSSVSGTVEKIESIIDTSGLYRNA
                     IYIQVHGDEWESNIDRTSDIVSKIDYTPQYILDKISQAGIVGMGGATFPLHVKLSPPD
                     TTKPEVLIINAVECEPFLTADHSLMLEKAEEICIGISLIKKVLGVDSALVGIENNKPD
                     AIALMQDIAHKYNGIGIVPLQIKYPQGGEKQLIEALLHKEVPSGQLPIVVGAVVVNVA
                     TAFAVYEAVQKNKPLIDRVVTVTG"
BASE COUNT          613 a          211 c          358 g          534 t
ORIGIN      
        1 taatgtaatt gaaaatccct tgatagatga ggttgaggct atgttacccg gagctaattg
       61 tggtggttgt ggatttcccg gatgtagaaa atttgctgat gttttggtaa attctgaatc
      121 tattgaaggt ttgttttgta gtccggcagg acaagaacaa atggaaaaaa ttgcagaaca
      181 tttaggaaaa tcggtttcaa aaaaggctat gcaagtagct gtagttcgtt gtaatggtag
      241 ttgcgacgta cggcctgtaa caaatgaata tgaggggatt tcctcttgtg catttgctgc
      301 acaactatat gcgggtgaaa cagcatgttc atttggatgc ttagggttag gagattgtga
      361 attagtatgt gcttttgatg ctattcatgt aaatgtaatt accggattac ctgaggtaat
      421 aacggataaa tgtacggctt gtggagcatg tgttgatgcg tgtcctaagc aaataattga
      481 attaagaaaa acgaataaaa aagatttaaa aatatatgtt tcttgtgtaa ataaagataa
      541 aggtgtagtt gctaaaaagg catgcgatgt tgcttgtata ggatgtggaa aatgtgttaa
      601 agtatgtcca cattccgcta ttacattaca aaactcttta gcttatatag atgatgaatt
      661 atgtaaattg tgtagaaaat gtgttgatga atgtccaact catgcaataa ttgaaaccaa
      721 ttttcctgaa aaaaagaaac aagttcaaga aaatagttca gatattgagt aattcgtatt
      781 atttataatt aatgaagaat aaataaaaaa ctatagatga aattaagaaa gaaatatgca
      841 atgcaaacat ttactaaagg aggaattcat cctttagagc ataaactttc cagtaagcaa
      901 cctattgaaa tgttgcctat tcccgaattt gttcaaatat cattatctca acatattggt
      961 gtagctgcta caccaatagt aaaagttgga gattcggtta aagtaggtac atgtataggt
     1021 gagtcagcag gatttatttc tgcgcgagta cattcatcgg tatcaggtac agtagaaaag
     1081 atagaaagta taattgatac ttcaggttta tatagaaatg caatatatat acaagttcat
     1141 ggtgatgagt gggaaagtaa tatagataga acttcagata tagtttcgaa aattgattat
     1201 actcctcaat atattcttga taaaatatcg caagcaggta ttgttggtat gggaggagca
     1261 acatttcctt tacatgtaaa attaagtccg ccagatacca ctaaaccaga ggttttaata
     1321 ataaatgcag tagaatgcga accattttta acagccgatc atagtttaat gttagaaaaa
     1381 gcggaagaaa tatgtattgg tatatcatta ataaaaaaag ttttgggagt tgattcggct
     1441 ttggtaggta ttgaaaataa taaacctgat gctattgcat taatgcaaga tattgctcac
     1501 aaatacaatg gcattggaat agttccattg caaattaaat atccgcaagg aggtgaaaaa
     1561 caattaatag aagctttatt acataaagaa gtaccttctg gtcagttacc aatagtagtt
     1621 ggagcggttg ttgtaaatgt agcaactgca tttgctgtat atgaagctgt acaaaaaaat
     1681 aaaccattaa tagatagagt ggttacagta acaggt
//
LOCUS       DAOTOW010000543         1715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54589_length_1715_cov_1.928916, whole genome shotgun sequence.
ACCESSION   DAOTOW010000543 DAOTOW010000000
VERSION     DAOTOW010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54589_length_1715_cov_1.928916"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5243_09035"
     CDS             <1..536
                     /locus_tag="P5243_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19637.1"
                     /translation="KGTTIAIEVPYTMYWDFGRSYNPEGKVVEYADRKEVVREEYMYG
                     PHQVTLTVENTHGCLSSITKEIFVDIKSGVYIPTAFAPTNPAFGVRYFQPLGYGFKTC
                     EVWIYDTWGNLLWYSDEVEDGIFVGKWDGTYNGELLKSDTYIWKMEATFADGKLWLGQ
                     GDNPNRLKKFGSVTLIR"
     gene            806..>1715
                     /locus_tag="P5243_09040"
     CDS             806..>1715
                     /locus_tag="P5243_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19638.1"
                     /translation="MKTRAYLPILQKSQMILRLLMFVLFVLISNYGNSQTCTNIVGGT
                     DFTPIPEHATDYYYHYNFVKQKGPSGNFISTPAALASAATDFSQSKFVYAVTSNPRKL
                     RSDFVNVEDSMMVIKLQTGTNSTLFNYKVSGLKPGSNYTVKVKIYHLPMYDSACHNSY
                     KWQQTNLRMAVNPDQYGNGFNDYSLQSEAASWGKDFTFTINGTLAAADNSINFEIRTG
                     YNFTTCSAIGIADILITGCLNPQIKSSKDKEVCTGEQTLLTLDKEYYANSYTWQKSTN
                     GGSTWTTISTAKSVIDAVTQNSVYRVN"
BASE COUNT          603 a          215 c          312 g          585 t
ORIGIN      
        1 caaaaggtac tacaattgca attgaagtac cgtatactat gtattgggat tttggtcgtt
       61 catataaccc tgaaggtaaa gttgtggaat atgctgacag aaaagaagtt gtaagagagg
      121 agtatatgta tgggccacat caagtaacat taactgttga aaatacacat ggttgtttga
      181 gttcaattac aaaagaaata tttgtagata ttaaatctgg tgtttatatt cctacagctt
      241 ttgcgccgac caatcctgct tttggagtta gatactttca acctcttgga tatggattta
      301 aaacatgtga agtatggata tatgatacgt ggggtaattt actatggtat tcagacgaag
      361 ttgaagatgg aatatttgta ggaaaatggg acggaacata caatggagaa ttgttaaaat
      421 ctgataccta tatttggaaa atggaagcaa catttgctga tggaaaattg tggctagggc
      481 aaggtgataa tccaaatcga ttgaaaaaat tcggttcagt tacgcttatt cgataattga
      541 taaaaaagta tttacaaaga agctgtttta taaaaatgtg aaacagcttc ttttttttta
      601 tataaatgca accttttgta ttgtttatat gtctgtatat ttaaaagata tgatacaatc
      661 acataattat ttttttatta tttttttctt tgtttgtttg ataataaagt attaatgatt
      721 gaaaaaataa tattgatttt gaatagtaat agtctttaat tttgtatatt gctaaattgt
      781 ataaaatcct aaataaaaaa tatatatgaa aacacgtgca tatttgccga ttttacaaaa
      841 atctcaaatg atattgcgtt tgttgatgtt tgtattgttt gttttaattt ctaactatgg
      901 aaattctcaa acttgtacta atatagttgg aggaactgat tttactccaa ttccagaaca
      961 tgctacagat tattattatc attataattt tgtaaaacaa aaaggtccat ccggaaattt
     1021 tattagtaca ccggcagcat tggcatcggc tgctactgat ttttcacaat caaaatttgt
     1081 atatgcggta acgtcaaatc cacgtaagtt acgaagtgat tttgtaaatg ttgaagatag
     1141 tatgatggta attaaacttc aaaccggtac aaattccacg ttgtttaatt ataaagtttc
     1201 aggtttaaag ccggggtcaa attatacggt taaagtaaaa atttatcatt tacctatgta
     1261 tgattctgct tgtcataata gttataaatg gcaacaaaca aatttaagaa tggctgtaaa
     1321 tcccgatcaa tatggaaatg gatttaatga ttattcattg caatctgaag ctgcaagttg
     1381 gggaaaggat tttacattta caataaatgg aacgttggcc gctgcagata attcaataaa
     1441 ttttgaaatt cgtacagggt ataattttac aacatgttct gcaataggta ttgcagatat
     1501 tttaattacc gggtgtttga atccgcaaat taaatcaagt aaggataaag aggtttgcac
     1561 cggtgaacaa acattattaa cactcgataa agaatattat gcgaattcat atacatggca
     1621 aaaatccact aatggaggct ctacctggac aacaataagt acggcaaaga gtgtgattga
     1681 tgccgtaact caaaattctg tatatagggt aaacg
//
LOCUS       DAOTOW010000544         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54610_length_1714_cov_3.053647, whole genome shotgun sequence.
ACCESSION   DAOTOW010000544 DAOTOW010000000
VERSION     DAOTOW010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54610_length_1714_cov_3.053647"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="P5243_09045"
     CDS             complement(<1..846)
                     /locus_tag="P5243_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS19639.1"
                     /translation="MKKTLFLSLFLGLCGVYALQAQQLSLSLSQAKQYAVSHNISIKN
                     AELSVAAAQKNVQSTIAKGLPQADASFDYSHFFNYELNLSFGSGGTTQPIDYSKLDAG
                     DAEIMKLLSSFGSSEPIVMKGSMSSKIQVSQILFSGQYWVGIQMAKLAKLMNEQNLEK
                     SRADIEESVSNVYFGILVLEESLGVIQTTIQNLKETQTRTSAMVRAGMAEATDTTKLK
                     MAVNNLVNNKKQLERTISFNYSMLKFLLGVNQQAAVTLTDPISSFIDEAAITSLLLKP
                     FSVEQV"
     gene            complement(950..1600)
                     /locus_tag="P5243_09050"
     CDS             complement(950..1600)
                     /locus_tag="P5243_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19640.1"
                     /translation="MNFDEQHIIKRVMHIFALYGVRSFSIDTIAMYIPLSKKELLKIA
                     KNKEELIAKIFEYRAIIAIEISKQIHENPYIVNAIDILVHMSVLMSKACNEFNTQLDF
                     EFKKYYPVLFAEYEKQKHAHIIQNMTAIIEQGQKELLFKSEIVVLQVAEHYFEQVQKH
                     HESLQEESKFSRQTIEFMIEHISQFIQSISTQLGIIHFESKKHILFDLLSEWDSKV"
BASE COUNT          562 a          284 c          213 g          655 t
ORIGIN      
        1 aacttgctct accgaaaaag gtttcaaaag taacgatgta atagcagctt catctataaa
       61 acttgaaatt ggatcggtaa gcgttacagc tgcttgttgg ttaacaccta ataaaaattt
      121 aagcatacta taattaaaac ttatagttcg ttctaattgc tttttattgt ttaccaaatt
      181 atttaccgcc attttaagtt ttgtagtatc agtagcctca gccattcccg cacgtaccat
      241 tgcagaagtt ctggtttgag tttcttttaa attttgtata gttgtttgta ttactcccaa
      301 agattcttct aatactaaaa ttccaaaata cacatttgaa acagactctt caatatcggc
      361 tctacttttc tcaagatttt gttcattcat aagttttgca agctttgcca tttgaattcc
      421 aacccaatat tgaccactaa agagtatttg tgaaacttga attttactac tcatagaacc
      481 tttcattaca ataggttcag aactaccaaa tgaactcaac aatttcatta tttcagcatc
      541 gcctgcatct aattttgaat aatcaattgg ttgggttgtt cctcccgaac caaacgataa
      601 attgagttca taattaaaaa aatgcgaata atcaaaactt gcatcagctt gaggtaatcc
      661 ttttgcaatt gttgattgca catttttttg agctgccgcc acagataatt cagcattttt
      721 aatactaata ttatgtgaaa ctgcatattg ttttgcttga gataaactaa gcgacaattg
      781 ttgtgcttgt agtgcataca ctccacacaa acccaaaaac agtgataaaa aaagtgtttt
      841 tttcatataa tttattttaa ttttttgttc aaaactagtt gttacctatt attttttcta
      901 ttcaaaatcg acaaaactct cttttttttc gactaacata tttttttaac tatacttttg
      961 aatcccattc agaaagcaaa tcaaacaata tgtgtttttt tgattcaaaa tgtattatac
     1021 ctagttgagt agaaattgat tgaataaatt gtgaaatatg ttcaatcata aactcaattg
     1081 tttgacgtga aaattttgat tcttcttgca atgactcgtg atgtttttgc acttgctcaa
     1141 aataatgctc agcaacttgc aatactacaa tttctgattt aaaaagtaac tctttctgtc
     1201 cttgttctat aatagctgtc atattctgta taatatgagc atgtttttgt ttttcatatt
     1261 ctgcaaacaa aacaggataa tactttttaa actcaaaatc taattgcgta ttaaactcat
     1321 tacatgcttt actcataagc accgacatgt gcactaaaat atctatagca tttactatat
     1381 aaggattttc atgtatttgt ttgctaattt caatcgcaat aatagctcta tattcaaaaa
     1441 tctttgcaat taattcttct ttgttttttg caatttttaa taattctttt ttacttaaag
     1501 gaatatacat agctatagta tctatactaa agcttcgcac accatataaa gcaaatatgt
     1561 gcattactcg cttaattata tgttgttcat caaaattcat aatacaaata tataatagat
     1621 tttttttaaa agacacgttt ttttaaattt tcgtttctta aacactgttg tccgataaaa
     1681 aattaagaga aggaagatgt tagtcttcct ttct
//
LOCUS       DAOTOW010000545         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54670_length_1713_cov_2.471653, whole genome shotgun sequence.
ACCESSION   DAOTOW010000545 DAOTOW010000000
VERSION     DAOTOW010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54670_length_1713_cov_2.471653"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="P5243_09055"
     CDS             complement(<1..590)
                     /locus_tag="P5243_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRS19641.1"
                     /translation="MNAILKDRNSQITRAGADTKTIACNKPSLAGAVSQRACVFCGSR
                     VVLYPIADALHLIHGPIGCAAYTWDIRGAMSSGPEIHRMSFSTDLHEKDVVFGGEKKL
                     EAALLELIDAYNPKAAFVYSTCIVGVIGDDIESICRKVQALKQIPVIPVHAPGFQGTK
                     KDGYRAACEALEVLVGTGNRPAQEFSINILGDFNLAG"
     gene            complement(729..>1713)
                     /locus_tag="P5243_09060"
     CDS             complement(729..>1713)
                     /locus_tag="P5243_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRS19642.1"
                     /translation="YVFHANTPSYVGSHVTGYANMVTAMVKTFSMKSSTKKNQVNIIS
                     GWIEPADMREIKRIANQMMAKIVLFPDTSDVLDAPMTGKHEFYPKGGVTIPELISTGD
                     SKFTIGLGPLCTLDACIKLENQCKIKFDSLELPIGIQATDAFITALSRAAGVQVPDSL
                     TDERGRLIDIIADMQKYLYGKRVALFGDPDTLVPLTEFLVALDMKPVYIVTGTPDKTF
                     EDRMASILSAKVPEAKYKAGERADIFLLHQWIKQQGVDLLIGNTYGKYIARDENIPFV
                     RLGFPILDRVGHSYFPMVGYAGAMNLVTKILDAIMDKIDREVPEEKVELVM"
BASE COUNT          487 a          354 c          328 g          544 t
ORIGIN      
        1 ccggcaagat taaaatcgcc caaaatattg atactgaatt cttgtgccgg acgattacct
       61 gtacctacta atacttccaa agcttcgcat gctgcacgat acccgtcttt tttggtacct
      121 tgaaagccgg gagcatgcac cggaattacc ggaatttgtt tcaaagcttg aactttgcga
      181 cagatacttt ctatatcgtc gcctataaca ccaacaatac aggtagaata tacaaatgca
      241 gccttaggat tgtatgcatc tattaattca agcaaagcag cttccaattt tttttctccg
      301 ccaaacacca catctttttc gtgcaaatcg gtagaaaaac tcattctgtg gatttcgggg
      361 cccgatgaca tagcgccccg aatatcccat gtatatgcag cacatcctat tggaccatga
      421 ataagatgta gggcatctgc tataggatac aatactaccc gcgagccgca aaatacgcat
      481 gctcgttgac ttaccgcacc tgctaaactt ggtttattgc aggctattgt ttttgtgtcc
      541 gcaccggctc gtgttatttg tgaattccgg tctttgagta ttgcattcat tgttgtacat
      601 gttttagaat aagtcctctt cttttcaact ttatcttgag ggcgagaaac tgatgagaaa
      661 gaagaggact aaggaataat tactatgaaa ctatgaggaa tagagtccta tatgaataga
      721 atttagaatt acataactag ttcaactttt tcttcgggta cctcgcggtc aattttatcc
      781 attattgcat cgagtatttt ggtaaccaaa ttcatagctc ctgcatatcc aaccataggg
      841 aaatagctgt gtcctacacg gtcaagaatc ggaaatccca aacgaacaaa cggaatattt
      901 tcgtcgcgtg ctatatattt accataggta ttacctataa gtaaatcaac gccttgttgt
      961 tttatccact ggtgcaatag gaatatgtcg gcacgttcac ctgctttgta ttttgcttct
     1021 ggtactttgg ccgataaaat acttgccata cgatcttcga atgttttgtc gggagtaccg
     1081 gttactatgt ataccggctt catatccaaa gctacaagga attcagttaa tggaactaat
     1141 gtatcggggt cgccaaataa agctactcgt ttgccgtata aatatttttg catatcggca
     1201 attatatcta ttaatctacc tcgttcatca gttaaagaat ctggtacttg tactccggca
     1261 gcacggctta gagctgtaat aaaagcatcg gtagcttgta ttccaatagg taattcgagt
     1321 gagtcgaatt taattttaca ttgattctct aatttaatac atgcatcaag agtacaaagt
     1381 gggccaagac ctatggtgaa tttactgtcg ccggtgctaa tcaattccgg tatggtaaca
     1441 ccaccttttg ggtaaaattc gtgttttccg gtcataggag cgtcaagcac atcagatgtg
     1501 tcgggaaaca gaactatttt tgccatcatt tgatttgcaa tacgtttgat ttcgcgcata
     1561 tctgccggtt caatccaacc acttataata tttacctgat tttttttggt tgacgatttc
     1621 attgaaaacg ttttgaccat tgcggtaacc atattagcat atccggtaac atgtgaaccc
     1681 acataacttg gtgtatttgc atggaataca tat
//
LOCUS       DAOTOW010000546         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54694_length_1712_cov_3.360290, whole genome shotgun sequence.
ACCESSION   DAOTOW010000546 DAOTOW010000000
VERSION     DAOTOW010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54694_length_1712_cov_3.360290"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1422)
                     /locus_tag="P5243_09065"
     CDS             complement(223..1422)
                     /locus_tag="P5243_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRS19643.1"
                     /translation="MKRIFIISIFILCKIYALYGFGLLIPMDDKQKNHLKAYGITYWV
                     LQHEIEVQWLLNYRGGSFMMEFNQSIKQECEIRGVSYEIIPDVKVQAIITEVSAPDVN
                     MDVVKLEKAPKIAVYSPKDKLPWDDAVTLALTYAEIPYDVIYDGEIIQGELSKYDWLH
                     LHHEDFTGQYGKFYAAYRNAPWYIDQVKNAEAQAKQYGFNKVSELKLYVAKTIKTYIA
                     NGGFLFAMCSGTDTFDIALAAENTDICESMFDGDPADPQAQSKLNYDNCIAFRNFNLV
                     KNPYEYEYSDIDATNTRKVKDDEDYFTLFEFSAKWDPVPAMLCQNHTNIIRGFMGQTT
                     AFNKEFIKPNVVIMGELQSANEARYIHGTHGKGTWTFYAGHDPEDFRHYVGDQHTNLD
                     NHKNSPG"
     gene            complement(1427..>1712)
                     /locus_tag="P5243_09070"
     CDS             complement(1427..>1712)
                     /locus_tag="P5243_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRS19644.1"
                     /translation="DGLSTKGLAEIIIAKNRHGAVDDVRLEFIPELTKFTDRNIIPEL
                     NDNIIDDITNIEKSFDTNFETKTFVSKMNQNQPIDISGDFDTTIDTNIPY"
BASE COUNT          547 a          276 c          238 g          651 t
ORIGIN      
        1 aaaaacaatt acaaaaaaaa tcaatagaac gaattgtaac cttttactca gataaaacct
       61 ttacagaata tattcctgaa taataatgca gaaaaattgc attaattttt agattattgt
      121 attatgagat tcaatggaat atactatatt ttaataaatg aattaggttt tttgtttttt
      181 cttttttgca gcaggaaata atacattgtt tagaataaga cgttagcctg gagaattttt
      241 atgattatct aaattagtat gttggtctcc aacataatgt ctaaagtctt caggatcatg
      301 tcccgcataa aatgtccaag tgccttttcc atgagttcca tgtatatagc gagcttcatt
      361 tgctgattgt aattcgccca taattacaac attaggtttt atgaattctt tattaaaagc
      421 agttgtttgt cccataaaac ctcgaattat attagtgtga ttttgacaca acatagctgg
      481 aacgggatcc catttagcag aaaattcaaa taatgtgaaa taatcttcat catcttttac
      541 ttttcgagtg tttgttgcat caatatcaga atattcatat tcatagggat ttttaacaag
      601 attaaaattg cgaaaagcaa tacagttatc ataatttaat tttgattgag cttgagggtc
      661 agcaggatct ccatcaaaca tactttcaca aatatctgta ttttcagctg ctaatgcaat
      721 atcaaaagta tcggttcccg aacacatagc aaataaaaaa cctccatttg caatatatgt
      781 tttaattgtc tttgctacat atagttttaa ttctgatact ttgttaaatc catattgttt
      841 tgcttgagct tcggcatttt ttacttgatc tatataccat ggtgcatttc tgtaggctgc
      901 atagaatttg ccgtattgac cggtaaaatc ttcatgatgt aaatgcaacc aatcatattt
      961 tgatagttca ccttgaatta tttctccgtc atatattaca tcataaggta tttctgcata
     1021 tgttaaagca agtgttacag catcatccca aggtaattta tctttgggag agtatactgc
     1081 tattttgggt gctttttcaa gttttactac atccatattt acatcgggag cacttacttc
     1141 tgtaataatt gcttgaactt tcacatcggg aattatttca taactcactc ctcgtatttc
     1201 acattcttgt tttatgcttt ggttaaactc catcataaaa ctcccacctc tataattcaa
     1261 taaccattgc acttctattt catgttgcaa tacccaatat gttataccat aagcttttaa
     1321 atgatttttc tgcttgtcat ccattggaat taagagtcca aacccataca atgcatatat
     1381 tttgcataaa ataaaaattg atattatgaa tattcgtttc attgaattag tatggaatat
     1441 tagtatctat agttgtatca aaatcaccag aaatatctat aggttgattt tgattcattt
     1501 tagagacaaa cgtttttgtt tcgaaattgg tatcaaaact tttctcaata tttgtaatat
     1561 catctataat gttgtcgttt agttctggaa ttatgtttct atcagtaaat ttggtaagtt
     1621 caggaataaa ttctaatcga acatcatcta cagcaccatg tctgtttttt gcaattataa
     1681 tttcagctaa gccttttgta cttaatccat ct
//
LOCUS       DAOTOW010000547         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54829_length_1709_cov_3.681983, whole genome shotgun sequence.
ACCESSION   DAOTOW010000547 DAOTOW010000000
VERSION     DAOTOW010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54829_length_1709_cov_3.681983"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="P5243_09075"
     CDS             complement(<1..1071)
                     /locus_tag="P5243_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS19645.1"
                     /translation="MRKVVVISVVSLLSWISVVGQGVVKGKITNELNEPVIAASVYLE
                     SNESYGVYSDTDGNYEIELPHNTPQRVVVSYLGYSNQTFEVTVKKGQVYLKNIKLVPS
                     QQALNTVVVTGKANLSNDMALEKMRMKTAASIDYISAATISKTGDANVIAAVSRVSGV
                     STYGGFITVRGIGDRYIRTTINGMSIPTLDPFTNNIKLDMFPTSLINNVIITKTYSAD
                     LPGDWAGAFLSVETKTNPNKLIASYESSYGYNSQTTFKEILTTQKSKTDWLGYDNGFR
                     EYDHDSFVNAKMKPETYDQFVALGLGDYFSSIGVTDYRTWNMNPVFYTKMGLIEMGLL
                     DKSQINDNEAYLSALSEFKSTYS"
     gene            complement(1227..>1709)
                     /locus_tag="P5243_09080"
     CDS             complement(1227..>1709)
                     /locus_tag="P5243_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19646.1"
                     /translation="KVGQSIQITANATDGDGTIAQFEFFVDGVSIGVDNTAPYSVNYT
                     GVLGSKTITAVAKDNDDQTTTSIAVQITVEEETSVEEITNSDITVYPVYTNSTVEVTV
                     PSASHIQYEIYSVLGVVVEKGIAHNSKFELDLSSLSSGQYIVKVIADSKSYLKRVVKK
                     "
BASE COUNT          540 a          315 c          244 g          610 t
ORIGIN      
        1 agaataggta gatttaaatt cacttaaagc agataaatat gcttcattat cattaatttg
       61 tgatttatca agtaatccca tttctattaa tcccattttg gtatagaata ccggattcat
      121 attccaagtt ctataatcgg taacacctat agaagaaaaa taatctccta aacctagtgc
      181 gacaaactga tcatacgttt cgggtttcat ttttgcattt acaaacgaat catgatcata
      241 ttctctaaac ccattatcat accctaacca atctgtttta cttttttgcg tagtaagaat
      301 ttctttaaat gttgtttggc tattgtatcc ataagaagat tcatacgatg caataagttt
      361 gtttggattt gttttggttt caaccgataa gaaagcacca gcccaatcac ccggtaaatc
      421 ggcactatat gtttttgtaa taattacatt gttgattaaa cttgtaggaa acatatcaag
      481 tttaatatta ttggtaaacg ggtccagtgt tggaatactc ataccattta tggtagtacg
      541 aatataacgg tcacctatac cacgcacagt tataaacccg ccataggttg aaacacccga
      601 aactcgcgat actgcagcaa ttacattggc atctcctgtt ttactaatag tagcagccga
      661 aatatagtca atacttgctg ctgttttcat acgcattttt tctaaagcca tatcattact
      721 taaattggct ttacctgtta ctactacagt gtttaatgct tgttgtgaag gaacaagttt
      781 tatatttttc aagtacactt gtcctttctt tacggttact tcaaaggttt gattactata
      841 ccctaaatat gatactacaa cacgttgtgg ggtattatgc ggcaattcaa tttcataatt
      901 accatcggta tcactgtata caccataact ttcatttgat tcaagataca cagaagctgc
      961 aattaccggt tcattcaatt cattggtaat ttttcctttt actactcctt gaccaacaac
     1021 acttatccaa cttaacaaag aaactacact tataactaca acttttcgca tattacgaat
     1081 ctatattcta taaaaaactg ttaatacttt ttaaaaaact caaaaccgaa accattgcat
     1141 tgtatgatta actctttaac caataaagct tggggcttgg tgcaatgatt tgcagttttg
     1201 agaggataca tatgaagaga gagatgttac tttttaacta cacgttttaa atatgatttt
     1261 gaatcagcaa taactttcac tatatattgt ccgctcgata gagatgataa atctaattcg
     1321 aatttagaat tatgtgctat tcctttttct acaaccacac ctaaaacact gtatatttca
     1381 tactgtatgt gcgatgccga aggaaccgta acttctactg ttgaatttgt ataaacagga
     1441 tatacagtaa tgtcactgtt tgtaatttct tcaacagaag tttcttcttc tactgtaatt
     1501 tgtactgcta tagaagtagt tgtttgatca tcattatctt tagcaactgc tgttatagtt
     1561 ttacttccta ataccccggt ataatttact gaatatgggg ctgtattatc tacacctatt
     1621 gaaactccat caacaaaaaa ttcaaattgt gctattgttc catcaccatc agtagcattt
     1681 gctgtaattt gaattgattg tcctacttt
//
LOCUS       DAOTOW010000548         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54853_length_1709_cov_2.301088, whole genome shotgun sequence.
ACCESSION   DAOTOW010000548 DAOTOW010000000
VERSION     DAOTOW010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54853_length_1709_cov_2.301088"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="P5243_09085"
     CDS             complement(<1..1644)
                     /locus_tag="P5243_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRS19647.1"
                     /translation="MNAKLGMGINLGNAYEATGLGEWGVNPDSTYFVDIAQKGFASIR
                     IPARWSAHALTQAPYTIHQYFMDTIEWAVKQSLKNGLYVVLDMHHYEEMFQEPAAHKE
                     RYLAMWEQIAEHFKDYSDSLYLEIFNEPHDNFTPELWNQYLLDGLEVIREKNPTRMVI
                     IGTAEYGNLSGLSKLQIPSTDTCLIVTVHYYNPFDFTHQGATFAGVTATNVKWDSTAA
                     QIQAVKNDMEIIKNYSISKNVPIYIGEFGVIQNADDISRARWAGHLRKVFENYGFSAA
                     YWEYCSGFGLYDYALDCYYNGLLKALTDSVGECDCAMYDTIIVKNSTFKRGLNPWVVY
                     KNEASGAQAQVEIINEEARLEIIKNSTVEWHIQFVYPSFPITMGSTYTMVFDVYSSSN
                     TPLTAQISLDGGTYAVIHDTLVNVTDTKQTFSTTFTHNEPSIKKARMVFECGTITSQY
                     LYFDNIHIYEVVKGVPVQTITIQLPESQTSAQITTKQGSLQLSADVVPANATNTDVVW
                     SIISGGQLATIDNTGLLTATGYGNGSVRVQALAYDGSGVK"
BASE COUNT          525 a          340 c          260 g          584 t
ORIGIN      
        1 ttttactcca cttccatcgt aagccaatgc ttgaactctt accgaaccgt ttccgtatcc
       61 ggtagcagtt aacagtcctg tattatcaat tgttgccaat tgtccaccac ttataattga
      121 ccatacaaca tcggtattcg ttgcattggc tggtaccacg tcggcagaaa gttgtaaact
      181 tccttgcttg gtagtaattt gagcacttgt ttgtgactca ggcaactgta tagttatagt
      241 ttgtacagga actcctttta caacttcata tatgtgtata ttgtcaaaat acaaatattg
      301 cgatgttata gtaccacatt caaataccat tcgtgctttt ttaatagatg gttcattgtg
      361 cgtaaatgtt gtagaaaacg tttgttttgt atctgttaca tttactaacg tgtcatgaat
      421 tacggcatag gttccgccat ctaaactaat ttgtgccgtc aaaggagtat tacttgatga
      481 atatacatca aacaccatag tataggtact tcccattgtt ataggaaatg atggatacac
      541 aaactgtata tgccattcta cagtactgtt tttaataatt tctaaccgag cttcttcatt
      601 tattatttca acttgagctt gcgcacccga tgcttcattt ttatatacta cccaaggatt
      661 taacccgcgc ttaaatgtac tatttttaac aataattgta tcatacattg cacaatcaca
      721 ttctcccacc gaatctgtta aagctttgag taatccattg taataacaat ctaatgcata
      781 atcatacaaa ccaaatccac tgcaatattc ccaatatgcc gcactaaacc catagttttc
      841 aaatacttta cgcaaatgac ctgcccatct ggcacgcgaa atatcatcgg cattttgaat
      901 tacaccaaat tcaccaatat atataggtac atttttagat attgaataat tttttataat
      961 ttccatatca tttttcactg cctgtatttg agctgcagta ctatcccatt tgacatttgt
     1021 agccgttact ccggcaaatg ttgctccctg atgagtaaaa tcaaaaggat tataataatg
     1081 tacggttact attaaacacg tatcggtaga gggaatttgc aatttagaca atccgcttaa
     1141 gttgccatat tcggcagtac ctattataac catgcgtgta ggattttttt cacgtattac
     1201 ttctaatcca tcaagtaaat actgattcca taattcagga gtaaagttat cgtgtggttc
     1261 attaaatatt tcaaggtata aactatctga ataatctttg aaatgctcag caatttgttc
     1321 ccacatagct aaatatcttt ctttatgtgc agcaggctct tgaaacattt cttcgtaatg
     1381 atgcatatcg agcacaacat acaatccatt tttaagtgat tgttttactg cccattctat
     1441 agtatccata aaatattgat gtatagtata gggtgcttgc gtaagggcat gtgccgacca
     1501 tcgagcggga atacgtatag aagcaaatcc tttttgtgca atatcaacaa aatatgttga
     1561 atcgggattt accccccact ctcctaaccc ggtagcttca tatgcattac ccaaattaat
     1621 tcccatacct aattttgcat tcatggtaaa tgcataattc tgagaaaata tttgtacagt
     1681 gtaaaataca cttactactg ttaaaaaaa
//
LOCUS       DAOTOW010000549         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_54938_length_1707_cov_2.949758, whole genome shotgun sequence.
ACCESSION   DAOTOW010000549 DAOTOW010000000
VERSION     DAOTOW010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54938_length_1707_cov_2.949758"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1707)
                     /locus_tag="P5243_09090"
     CDS             complement(<1..>1707)
                     /locus_tag="P5243_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19648.1"
                     /translation="DSDTIVKRVATSHPFFVCKLNASFQKQWVKQAVSIGGNNYVSNV
                     MYGLDAQNNMYIGGTLGYVTGAVSVDGVIVKNDTILDEYDYTYTDLFLYKIDAQGTVQ
                     LGKTFLFRGTEQLADMKVLSDGSLYMVGDYSGEFKAMPYGDFPGASATLYYNTFIMRV
                     NGSNGSIIWGQPIVADVYYQERFRTIRVDADENVYVASRFRNPQVVFMGNTYYKRTDN
                     AYATQILFAKINKDGVKQWANVLGATTSFPEVIQHQSIGYWSIQDTTMILSIPYMNYG
                     TNKNVEWGDALVPTVSVQGTMFDNVLLLSANTGNVYKFFNRGYESLIQIDSISYFGVR
                     NEFEYFEVHKITSKTNSIQGSVYVNGAPYTNSIYTYLELISVMPGETGTIKQWATLNQ
                     SGEFSFDNVPYGGEYIISIAPNDSTIMCGYYSKLGYALWNQADTVRSEEQNDNLILTL
                     QKITYPTGTATIRGTLVIEALPNQSIASIDNVQVILRTQQKSTNIVAFTRAYYDYFGM
                     TQTYTYEFKNIPKGEYEIEVLYPFATSSETISVSITQDNQVIEDQDFRIVNNTVLKRF
                     GSF"
BASE COUNT          564 a          321 c          223 g          599 t
ORIGIN      
        1 aaaagaacca aatcgtttta acactgtatt atttacaatt ctaaaatctt gatcttctat
       61 aacctgatta tcttgtgtaa tacttacaga tatagtttca cttgaagttg caaacggata
      121 caacacttct atttcatatt caccttttgg tatattttta aattcatatg tgtatgtttg
      181 tgtcatacca aaataatcat aatatgctcg tgtaaatgca acaatattgg ttgatttttg
      241 ttgtgtacgt aatatcacct gcacattatc tatacttgca atagattgat ttggcaacgc
      301 ctctattaca agcgtacctc gtattgttgc cgttccggta ggatatgtaa ttttttgtaa
      361 cgttagaatc aaattatcat tttgttcttc tgaacgaaca gtatctgcct gattccataa
      421 tgcatatcct aattttgaat aatatccaca cataatagtt gaatcattag gagcaataga
      481 aattatatat tcaccgccat agggcacatt atcaaacgaa aactctcccg attgatttaa
      541 tgtagcccat tgtttaattg ttcctgtttc tccgggcatt acactaatta attctaaata
      601 cgtatatata ctatttgtat atggagcacc atttacatac acactacctt gtattgaatt
      661 tgtttttgag gttattttat gaacttcaaa atattcaaat tcattgcgaa cgccaaaata
      721 tgaaattgaa tcaatttgta taagcgattc atatcctcta ttaaaaaatt tatacacatt
      781 tccggtattt gcagaaagca gcaatacatt gtcaaacatg gttccctgaa cagaaacagt
      841 tgggactaat gcatcacccc actcaacatt tttattggta ccgtaattca tatatggaat
      901 tgaaagaatc atagtagtat cttgaattga ccaatagcca atagattgat gttgtataac
      961 ttcagggaat gaggtagtgg cacctaaaac atttgcccat tgttttacac catctttatt
     1021 aatttttgca aataaaattt gagttgcata tgcattatcg gttcttttat aatatgtatt
     1081 acccataaat actacttgag gatttctaaa tcgtgaagca acatatacat tttcatcggc
     1141 atctacacga atggttctaa atcgttcttg ataatataca tcagcaacta ttggctgccc
     1201 ccatataata gaaccatttg aaccattcac acgcatgata aatgtgttat aatataaagt
     1261 tgcacttgca cccgggaaat caccatatgg catagcctta aattcaccgg aataatcacc
     1321 aaccatatac aaactaccgt cagacaatac tttcatatct gccaattgtt cagtacctct
     1381 gaataaaaat gttttaccta attgtaccgt accttgagca tctattttat ataaaaacaa
     1441 atctgtatat gtataatcat attcgtctaa aatagtatca ttttttacaa ttacaccatc
     1501 tactgatacc gctcctgtta cataccctaa tgttccacct atatacatat tattttgagc
     1561 atctaaccca tacattacat tactcacata attatttcca cctatagata cagcttgttt
     1621 aacccattgt ttttgaaaac ttgcattaag tttacacaca aaaaacggat gcgaagttgc
     1681 aactcttttt acaattgtat cagaatc
//
LOCUS       DAOTOW010000550         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_55262_length_1701_cov_2.555893, whole genome shotgun sequence.
ACCESSION   DAOTOW010000550 DAOTOW010000000
VERSION     DAOTOW010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55262_length_1701_cov_2.555893"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..640
                     /locus_tag="P5243_09095"
     CDS             59..640
                     /locus_tag="P5243_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRS19649.1"
                     /translation="MNTFELYCKIGIEHIADFKGYDHILFIITLCGVYLLKEWKSILI
                     LITAFTIGHSVSLALAVLGFVSVSSELIEFLIPVTIVITSAGNLFKKPGTISPVLQKV
                     KYVLALFFGLIHGLGFSNYLKALLEKTELVLPLLSFNIGIEVGQLIIVTSIITIATVL
                     TRVGNVAQRDWNLVISGAGLGISFIMAIERFPW"
     gene            658..1311
                     /locus_tag="P5243_09100"
     CDS             658..1311
                     /locus_tag="P5243_09100"
                     /inference="COORDINATES: protein motif:HMM:NF020922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRS19650.1"
                     /translation="MKEILDFLKLLCVNNNREWFHANKALYDARKLQFEAIVEKSISD
                     ISQFDSAVKGLTAKDCIYRIHRDIRFSADKTPYKNHIAAYIAPGGKKSYAGGYYLHIE
                     PENSMIAGGIYMPPPDVLFTLRTHVHNNCDEFLRIVTNESFVQTFGSVQGETLQSMPR
                     GFSKDSPCAAYIKHKSLLLSVAVSDEQIESPDFYDYFLDVCKHMKEFNSFCNSAICI"
     gene            1302..>1701
                     /gene="arfB"
                     /locus_tag="P5243_09105"
     CDS             1302..>1701
                     /gene="arfB"
                     /locus_tag="P5243_09105"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRS19651.1"
                     /translation="MYIADCSSEFVFTASRSSGAGGQNVNKVNSKVELRFHIDSSSCL
                     TEEQKALLQAKLRSHITDDGYLCIRSQVSRSQLQNKEICVQKFYEYIAGALQVSKPRK
                     ATKPTQASQIERLHKKKLNSEIKKMRKSPDI"
BASE COUNT          571 a          239 c          327 g          564 t
ORIGIN      
        1 ttttagaaaa ggtattataa agcaacattt tgtaatacga tattaatacg atattaatat
       61 gaatacattt gaattatatt gtaaaatagg tatagagcat atagcagatt ttaaaggata
      121 cgatcatatt ttgtttatta taacattatg tggagtatat ctgctcaaag agtggaaatc
      181 aatactaata ttaattactg catttacaat aggacatagt gtttcgttgg cattggcagt
      241 attaggtttt gtatctgttt cgtcagaatt aatagagttt ttaatacccg ttactatagt
      301 tataacatct gcaggtaatc tgtttaaaaa gccgggaacc atatcgccgg tattacaaaa
      361 agtaaaatat gtattagcgt tattctttgg tcttattcat ggtttagggt tttctaatta
      421 tttaaaagca ttgcttgaaa aaactgaatt ggtgttaccg cttttatctt ttaatattgg
      481 gatagaagtg gggcaattaa ttattgtaac atcaataata acaatagcta cggtattaac
      541 tcgtgttggt aatgttgcac agcgagattg gaatttagtt atatcgggtg ccggtttagg
      601 aatttcgttt attatggcta ttgaacgatt tccttggtaa tgtaaaaatg aatgattatg
      661 aaagaaatat tggattttct taagttgttg tgtgtgaata ataatcgtga atggtttcat
      721 gcaaataaag ctttgtacga tgctcgaaaa ttacaatttg aagcaattgt agaaaaaagt
      781 atttccgata tatctcaatt tgattcggca gtaaaaggtc ttaccgccaa agattgtata
      841 tatcgtattc atcgcgatat tcgattttcg gctgataaaa caccatataa aaatcatatt
      901 gcagcatata ttgcacccgg aggtaaaaaa tcgtatgccg gaggatatta tttacatata
      961 gaacctgaga attctatgat agcaggtggt atttacatgc cgccccccga tgttttgttc
     1021 acattgcgaa cacatgtgca caataattgt gatgaatttt tacgaatagt tacaaacgaa
     1081 tcatttgtgc aaacgtttgg ttctgtccaa ggcgaaacat tgcagagtat gccacgcggt
     1141 ttttcaaaag attcgccatg tgccgcatac attaagcaca aaagtctatt gttgtcggta
     1201 gctgtaagtg atgaacaaat agaatcaccg gacttttatg actatttttt agatgtgtgt
     1261 aagcacatga aggagtttaa ttcattttgt aattctgcaa tatgtatata gccgattgca
     1321 gttcagagtt tgtgtttaca gcttcgcgaa gtagtggggc aggtggacag aatgtaaata
     1381 aagttaactc taaagttgaa ttacggtttc atattgattc cagtagttgt ttaacagaag
     1441 aacaaaaagc attactacaa gctaaattac gttcgcatat taccgacgac ggctatttgt
     1501 gtatccgttc gcaagtatca agaagtcagt tgcagaataa agaaatatgt gtgcaaaaat
     1561 tctatgagta tatagcaggt gcattacaag tttctaaacc tcgaaaagca accaaaccta
     1621 ctcaggcttc acaaattgaa cgattacaca aaaagaaatt gaattcggaa attaaaaaaa
     1681 tgcgaaagtc acccgatatt t
//
LOCUS       DAOTOW010000551         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_55334_length_1699_cov_3.679440, whole genome shotgun sequence.
ACCESSION   DAOTOW010000551 DAOTOW010000000
VERSION     DAOTOW010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55334_length_1699_cov_3.679440"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="P5243_09110"
     CDS             complement(<1..850)
                     /locus_tag="P5243_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19652.1"
                     /translation="MLPNVNPTTTEAWKALEKYYATFKGTHMKDLFAADAQRFSKYSL
                     TFENTLVDFSKNLIDETSFSLLIKLAEECGLKQAIEAEFTGEKINKTEDRSVLHIALR
                     NRSNTPIYSDGVDVMPGINAVLAQMKDFSEQLISGSWKGYTGKAITDIVNIGIGGSDL
                     GPLMVTEALKPYKKPNINAHFVSNVDGTHIAETLKKVNPETTLFIVASKTFTTQETMT
                     NAETAKAWFLAAAKNEAYVKNHFVALSTNAKAVAAFGIDTKNMFEFWDWVGGRYSLWS
                     AIGLSIA"
     gene            complement(957..1241)
                     /locus_tag="P5243_09115"
     CDS             complement(957..1241)
                     /locus_tag="P5243_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19653.1"
                     /translation="MQKINTFWFGLLCGLVIPPITLLAIFIFFTKTMTFNEFLAILKS
                     WDLATNIYIWTIIPVFFLFSFFYYKKLDNASKGIVLPTMIYTILLVIMNM"
     gene            complement(1243..>1699)
                     /locus_tag="P5243_09120"
     CDS             complement(1243..>1699)
                     /locus_tag="P5243_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRS19654.1"
                     /translation="AAREGAINGIPSIAFSSLDFSPDADFDPFRPYIQTIATHVIKHK
                     LQPNVFLNVNFPCHDTANIQGIQVCRQTKGIWVEEFDKRTDPHNQDYFWLTGHFNNYE
                     PQATDSDEWLLKQNFVTAVPLQVETTDLQSLQLLHSLNTKSIIHHSSIL"
BASE COUNT          556 a          258 c          292 g          593 t
ORIGIN      
        1 aagctataga taatccaata gctgaccata acgagtaacg accgccaacc cagtcccaga
       61 actcgaacat gtttttagtg tctattccaa atgcagcaac tgcttttgca ttggtactta
      121 aagcaacaaa atgatttttt acatatgctt cgtttttggc agcagctaaa aaccatgctt
      181 ttgcagtttc ggcattggtc atagtttctt gcgtagtaaa tgtttttgaa gctacaataa
      241 ataaggtagt ttcaggattt acttttttaa gcgtttctgc tatgtgtgta ccatctacgt
      301 ttgatacaaa atgagcatta atatttggtt ttttgtaagg tttcaatgct tcggtaacca
      361 tgagtggacc taaatcggaa cctccaattc caatatttac aatatcggta attgctttgc
      421 ctgtatatcc tttccatgaa ccggagataa gttgttccga aaaatctttc atttgagcta
      481 aaacagcatt tattccgggc attacatcta caccgtcaga ataaattggg gtattgcttc
      541 tgttacgcaa tgctatgtgc aatacagagc ggtcttcggt tttatttatt ttttcgccgg
      601 taaattctgc ctctattgct tgttttagtc cacattcttc tgccaattta attaatagag
      661 agaatgaagt ttcgtcaatt aaattcttag aaaaatcaac taatgtattt tcaaatgtaa
      721 gtgaatattt agaaaaacgt tgtgcatcag ctgcaaacaa atctttcatg tgcgtacctt
      781 taaaagtagc atagtatttt tccaaagctt tccaagcttc ggtagttgta ggattaacgt
      841 ttggtaacat attataaagt tttaaattct gtttatactg ccacaaatgt acatgtttca
      901 aattattttc gttaggtgaa acgtaacatt ttttttgaat gaataacaaa ttattattac
      961 atattcatta ttacaagtag tatagtgtat atcatggttg gtaatacaat tccttttgat
     1021 gcattatcta attttttgta ataaaagaaa gaaaatagaa aaaatacagg aattattgtc
     1081 caaatataga tattagttgc taaatcccaa cttttaagaa ttgcaagaaa ttcattgaaa
     1141 gtcatggttt tagtaaaaaa aatgaaaatt gccaacagtg taattggggg aattacaaga
     1201 ccacacaata atccaaacca aaatgtatta attttttgca tattataaaa tagaagaatg
     1261 atgaataatt gatttggtat ttaatgaatg tagtaactgt aatgattgta aatcagtagt
     1321 ttcaacttgt aaaggcactg ctgttacaaa attttgtttg agtaaccatt catcactatc
     1381 ggtagcttgt ggttcataat tgttaaaatg tccggtaagc cagaaataat cttgattgtg
     1441 agggtctgtt cgtttgtcaa attcttcaac ccaaatacct ttagtttgac ggcatacttg
     1501 tattccttgt atattagcag tatcgtgaca aggaaaattt acatttaaaa aaacattggg
     1561 ttgaagtttg tgtttaatta catgtgtagc aatggtttga atatatggac gaaaagggtc
     1621 aaaatcggca tcggggctaa aatctaaact tgaaaatgct atagaaggaa ttccattaat
     1681 agctccttcg cgagcagca
//
LOCUS       DAOTOW010000552         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_55423_length_1697_cov_3.402558, whole genome shotgun sequence.
ACCESSION   DAOTOW010000552 DAOTOW010000000
VERSION     DAOTOW010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55423_length_1697_cov_3.402558"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5243_09125"
     CDS             <1..205
                     /locus_tag="P5243_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19655.1"
                     /translation="DNTLVKLMLVDSNGQHKDQRNANRLEAAQKLIEKTQIADKQREE
                     SDWHKQQSEYLKNKVNLETYINQ"
     gene            209..433
                     /locus_tag="P5243_09130"
     CDS             209..433
                     /locus_tag="P5243_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19656.1"
                     /translation="MAYNKKNKYRKIIEIQNIVNEYKAKGVTQEFIYKNYIKDVYFIS
                     RDCFYSYLATNAKRELKKLEEIDNMQKSLF"
     gene            467..1393
                     /locus_tag="P5243_09135"
     CDS             467..1393
                     /locus_tag="P5243_09135"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS19657.1"
                     /translation="MNQELKTKIAESLKSYLSAHGMSQNKFCEQTGINVAYVSALCNG
                     EHKVGDTPIASKYYTIISEKIGISIAKEFWKPVVTVQLQRVLATLEEAQEYGYTNIII
                     GETGSGKTYATDLFCASHPLDVYKVTVSQQDTIGDLIDKVLEKIKVPSSKSRSKRIND
                     IVKHMRNLKHQGYKPMLIFDEAEYMKQPSLCAMKEFYDNLNGVCSIVMIGTNQLLRSI
                     DKLRKKNTDGIPQLYRRIKFGIRELSPIDRSFKDFADLIQDKGVLQFVRTHCDNFGEL
                     HDVLVPALREADRLGENITENLVRTILNMPKL"
     gene            1395..>1697
                     /locus_tag="P5243_09140"
     CDS             1395..>1697
                     /locus_tag="P5243_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19658.1"
                     /translation="MKRAFSPTEVITMKKRVFNFENEFYEAFETPETCGVWFIWGNSG
                     NGKTSFVMQLAKELCKHAKVCYNSLEEGCSLTMRNALSRYEMTDVNKRFQLLDCEPM"
BASE COUNT          627 a          286 c          329 g          455 t
ORIGIN      
        1 tgacaataca ctggtaaaac ttatgcttgt agatagtaac gggcaacata aagatcagcg
       61 taatgcaaac cgcttagaag ctgctcaaaa gcttatagaa aaaacacaaa tagctgacaa
      121 acaacgcgaa gaaagtgact ggcacaagca acaatctgaa tacctgaaga ataaagtgaa
      181 tttagaaaca tatataaatc agtaatatat ggcatacaat aagaaaaata agtatcgcaa
      241 aattattgaa attcaaaaca tagtgaatga atataaggcg aaaggagtaa cacaggagtt
      301 tatatacaaa aactatatca aagatgtgta cttcataagt cgcgattgtt tttattctta
      361 tttagcaact aatgctaagc gagagcttaa gaagttggag gaaatagaca acatgcaaaa
      421 aagccttttt taaaccattt ttataatctt tttaaactat ttttttatga accaagaatt
      481 aaaaacaaaa atcgccgaat cgcttaaatc atatttaagc gcacacggta tgagtcagaa
      541 taaattctgc gaacaaacag gtataaatgt agcctatgta agtgctctgt gtaatggtga
      601 gcataaagta ggcgatacac ctatagcctc taaatactac actataatat cagaaaaaat
      661 aggtatttca atcgctaaag aattttggaa gccggtagta actgttcaat tacaacgagt
      721 acttgcaaca cttgaagaag ctcaggaata tggctatacc aatataatta ttggcgaaac
      781 cggttcgggc aaaacgtatg ctaccgattt gttttgtgca agccacccgc tcgatgttta
      841 taaagtaacc gtatcgcaac aagatactat aggcgacctt atagataagg tgcttgagaa
      901 aataaaagtg ccaagttcta aaagccgtag taaacgcata aacgatatag taaaacacat
      961 gcgtaacctt aaacaccaag gttacaaacc tatgcttata ttcgacgagg cggagtatat
     1021 gaaacaacca agcttgtgtg caatgaaaga attttacgac aatttgaatg gtgtatgttc
     1081 tattgtaatg ataggtacaa atcaactttt acgcagtatt gataagcttc gcaaaaaaaa
     1141 cacggacggc ataccgcaat tgtatcgccg cattaaattt ggcatccgcg aattgtcgcc
     1201 aatcgataga agctttaaag atttcgcaga tctaattcaa gacaaaggtg tactacagtt
     1261 tgttcgtacg cattgcgata attttggaga attgcacgac gttttggttc ctgcactacg
     1321 cgaagcagat agactaggag aaaatataac agaaaacctt gtacgcacca ttcttaacat
     1381 gcctaaatta tagtatgaaa cgagcattct cacctacaga ggttataacc atgaaaaaac
     1441 gagtgtttaa ttttgaaaat gaattttatg aggcgtttga aactcccgaa acatgtggcg
     1501 tgtggttcat atggggaaac agcggcaacg gcaaaacttc gtttgttatg cagcttgcta
     1561 aagaattgtg taaacacgct aaagtgtgtt ataactcttt ggaagaaggg tgtagtctta
     1621 ccatgcgtaa tgcgttgagt cgatatgaaa tgactgatgt aaacaagcgt tttcaactgc
     1681 tcgactgtga accaatg
//
LOCUS       DAOTOW010000553         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_55591_length_1694_cov_2.430750, whole genome shotgun sequence.
ACCESSION   DAOTOW010000553 DAOTOW010000000
VERSION     DAOTOW010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55591_length_1694_cov_2.430750"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="P5243_09145"
     CDS             complement(<1..1006)
                     /locus_tag="P5243_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19659.1"
                     /translation="MIIHENTYCITFKELTDGIVSVSNYKKMKHKGKIQVINRGCYGT
                     EALISFDSLPRKIKEQVVAKYGNPHDSDKNKYLLRNNIITDGLAESFFSSYILPDGRH
                     LPSDTQLEYTANASVLNAINILINDRTMFRKTLGHGCRDVWKLMGEESNRLKMEYKHT
                     LPENHRRLKQKLEEYKIHGYDVLVSKKFNNRNSAKVISEDQEAMLRGLLRYHTNLDNE
                     QIRDKYNIVACECGWDTITAATVGNYRSKWRVEITHGQRGKSYFDNTIAMQNRRRRTT
                     TPMTFWSIDGWDVELLYQRTDTNKKGQKTTTYHNRLTAVIILDTYCNYPIGYAIGDAE
                     N"
     gene            1036..1320
                     /locus_tag="P5243_09150"
     CDS             1036..1320
                     /locus_tag="P5243_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19660.1"
                     /translation="MKQIVFVVYSSSEQRPCRDLILQKFAAIFQTTFELNENFPDVCQ
                     LGVEETSTQIDHYRYSIDLLCNLWGFDYKEQVRVPFDEFLKDLNDGLVNL"
     gene            complement(1310..1495)
                     /locus_tag="P5243_09155"
     CDS             complement(1310..1495)
                     /locus_tag="P5243_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19661.1"
                     /translation="MEKSTKYQLPTILPHGWRNEVSKLLGIHRMTVYKALQRGENDAM
                     YKKIMHVAAQKYGKPTN"
BASE COUNT          490 a          273 c          294 g          637 t
ORIGIN      
        1 gattttcggc atcgcctatt gcgtagccta tagggtagtt gcaataggta tctaatataa
       61 ttacagctgt taacctgtta tgataagttg tagttttttg tccttttttg ttagtgtctg
      121 ttcgttgata tagtagctca acatcccaac catcaatact ccaaaatgtc ataggagtag
      181 tggttcttct tcggcgattt tgcatagcaa ttgtgttgtc gaaatatgat tttccacgtt
      241 gtccgtgtgt tatttcaaca cgccatttgc ttctataatt accaacagta gcagcggtta
      301 tagtatccca accacattcg caggcaacta tattatattt atctcttatt tgctcattat
      361 ctaaatttgt atgatatctg agcaatccac gtaacatagc ttcttgatct tcgcttatta
      421 cttttgcaga atttcggttg ttgaattttt tagaaactaa aacatcataa ccgtgtattt
      481 tatattcctc gagtttttgt ttaagtctgc gatgattttc cggcaatgtg tgtttgtatt
      541 ccattttaag gcggttagat tcttcaccca tcaacttcca aacgtctcta caaccatgtc
      601 caagtgtttt tcgaaacatg gttctgtcgt ttatcaaaat gtttattgcg ttcaaaaccg
      661 aggcattagc tgtgtattca agttgagtat cggaaggaag gtgtcttcca tctggaagaa
      721 tgtaagaaga gaaaaaactt tcagctaagc catcggttat gatattgtta cgtagtaagt
      781 atttgttttt atcactatcg tgtgggttgc catattttgc aacaacctgt tctttaattt
      841 ttcggggcaa actgtcaaat gatattaatg cctcggtacc gtaacaaccg cgattaataa
      901 cttgaatttt acctttgtgc ttcattttct tataattaga tacagaaacg ataccatcgg
      961 taagttcttt aaatgttatg cagtatgtat tttcgtgtat tatcattttg tatatttgaa
     1021 caaaaatttc atattatgaa acagattgtt tttgttgttt actcatcctc cgaacagcgt
     1081 ccttgtagag atttaatcct acagaagttt gctgcaattt ttcaaacgac ctttgagcta
     1141 aacgaaaatt ttcccgatgt ttgtcaactc ggtgttgagg aaacaagcac acaaattgac
     1201 cattatcgtt atagtattga cttattgtgc aatctgtggg gttttgatta taaagaacaa
     1261 gtacgcgtac catttgatga atttttaaaa gatttaaatg acgggttagt taatttgtag
     1321 gtttaccata tttttgggca gctacgtgca ttattttttt atacatagca tcattttctc
     1381 ctcgctgcaa tgctttatat acagtcattc tgtgtatacc aagaagtttt gaaacttcat
     1441 tccgccaacc gtgtggcaaa attgtaggta attgatattt tgttgacttt tccattttgt
     1501 atagtatatt tgtttaatta ttcatttgaa tacaaaagca aatatagaga aatactctaa
     1561 taaaaacaaa atttaattag agaaattttc taaatatttt atattatgtc tgtaaaagag
     1621 agaattagag ctgtctgtaa agacaaaaaa atgagcgttt atgagtttga gaaacttatt
     1681 gatgcatcta atgg
//
LOCUS       DAOTOW010000554         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_55657_length_1693_cov_1.891331, whole genome shotgun sequence.
ACCESSION   DAOTOW010000554 DAOTOW010000000
VERSION     DAOTOW010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55657_length_1693_cov_1.891331"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="P5243_09160"
     CDS             complement(<1..331)
                     /locus_tag="P5243_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19662.1"
                     /translation="MKILIGQHKLQLELWFGFLILLGCVLAYFQLLSNLTTNYHPVWS
                     DEFGYYINTQSFFENSTLKAAITLSGEGSAVFGADPHGFAYPLFHGCIAKIFGWSNLN
                     FIITNFIL"
     gene            complement(354..632)
                     /locus_tag="P5243_09165"
     CDS             complement(354..632)
                     /locus_tag="P5243_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS19663.1"
                     /translation="MPQTGIKDAIVSYLTFLFDPFILSGFVSAFVASVFWMIAMTKFE
                     ITVAYPFMSLAPAIVFILGVWVLNEPFTLGKVIGLLCIMVGIVVTVKF"
     gene            complement(760..978)
                     /locus_tag="P5243_09170"
     CDS             complement(760..978)
                     /locus_tag="P5243_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRS19664.1"
                     /translation="MKERILLVFKRTFELETVDESISQKNCDKWDSMNHLNLIVEFET
                     EFEITIEPEEMGIMKDFKTIETIISRKL"
     gene            complement(975..>1693)
                     /locus_tag="P5243_09175"
     CDS             complement(975..>1693)
                     /locus_tag="P5243_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19665.1"
                     /translation="IKQVLPMVAVPEFPVQPYLLPIFFEQLQNDYFSVYSITEEDKTK
                     TEQYKANAQRESMQHNFTNITDYLASLNINITIQKADDFNIPRIAQLTQKTNQFNLTT
                     KRYTESNIHEFIHEGGLVFCINVKDIFGDNGIVGVIILKKIDEVSFEIDSLLLSCRVL
                     GKGIEKAFVLSILNILKKQSVRRVSAQYFPTNKNVQVKDFYERIGFMLNNEKSESKSY
                     TIILEDNDFIIEPYLKIEIK"
BASE COUNT          582 a          303 c          222 g          586 t
ORIGIN      
        1 gcaaaataaa atttgttatt ataaaattca aattactcca accaaatatt tttgcaatac
       61 aaccatgaaa taacggataa gcaaaaccat gagggtctgc accaaagaca gcacttcctt
      121 ctccagataa ggttatagca gctttgagtg tactattttc aaaaaaagac tgcgtattta
      181 tataatagcc aaactcatca ctccaaacag gatgataatt cgtagtaaga ttgcttagta
      241 attgaaaata cgccaaaaca caccctaata aaatcaaaaa tccaaaccac aattctaatt
      301 gtaacttatg ttgacctata agaatcttca tttttttcat caatcattta ccattaaaac
      361 ttcaccgtca caactatccc caccattata cacaataagc caataacttt acctagagta
      421 aacggttcgt tcaacaccca aacgccaagt ataaacacta tagcaggagc aaggctcata
      481 aatggatacg ctactgtaat ttcaaatttt gtcatagcta tcatccaaaa taccgaagct
      541 acaaatgccg aaacaaaacc agaaagtata aatgggtcaa ataaaaaggt aaggtacgaa
      601 actatggcat ctttaatccc tgtttggggc aaactccacc cgagtttagt tatacgccat
      661 tttaaaatta tttgcccgta taccgtaaaa aaaagtgtac cgaatatgta gaggtagttc
      721 attggttatt attgatattg tagttttgcc aatagaatat tataattttc tcgaaattat
      781 tgtttctatt gttttaaaat ctttcattat tcccatttct tcgggctcta tagtgatttc
      841 aaattctgtt tcaaattcaa caatcaaatt caaatgattc attgaatccc atttatcgca
      901 atttttttgt gatatagatt catctactgt ttctaattca aatgtacgtt tgaaaactaa
      961 taatattctt tctttcattt tatctcaatt tttaaatatg gttcaattat aaaatcatta
     1021 tcctccaata taatagtata actttttgat tcactttttt cattgttcag cataaagcct
     1081 attctttcat aaaaatcttt tacttgcaca tttttattgg tagggaaata ttgtgcggaa
     1141 actcttctaa ccgattgttt ttttaagata ttcaatatag acaagacaaa tgccttttct
     1201 attcctttgc ccaaaacacg acaacttaac aataaggaat caatctcgaa cgaaacttca
     1261 tctattttct ttaaaattat aacaccaaca ataccattat caccaaagat atcttttaca
     1321 ttaatacaaa acaccaagcc accttcatgt ataaattcat gtatattgct ttcggtatac
     1381 cgctttgttg ttagattaaa ttggtttgtt ttctgcgtta gttgtgctat acgcggaata
     1441 ttgaaatcgt cagctttttg aattgttatg ttaatattta aacttgccaa atagtcggta
     1501 atattggtga aattatgttg cattgattcg cgttgtgcgt ttgctttata ttgttccgtt
     1561 ttcgtcttat cttcctcggt tatagaataa acactaaaat agtcattctg taattgttca
     1621 aaaaatatag gtagaagata tggctgaacg ggaaattctg ggacagcaac cataggcaaa
     1681 acttgtttta taa
//
LOCUS       DAOTOW010000555         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_55784_length_1690_cov_2.647095, whole genome shotgun sequence.
ACCESSION   DAOTOW010000555 DAOTOW010000000
VERSION     DAOTOW010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55784_length_1690_cov_2.647095"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="P5243_09180"
     CDS             <1..256
                     /locus_tag="P5243_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19666.1"
                     /translation="VLFFGFGIFTFSQFGGTVALGALVSVTLLMAMFSNVILLPALLL
                     TLEKAITNRHFKEPLLHIYNEEEDIDIEELKIISKYTNEI"
     gene            246..1199
                     /locus_tag="P5243_09185"
     CDS             246..1199
                     /locus_tag="P5243_09185"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS19667.1"
                     /translation="MKSKIKILVTGGAGFIGSSLIERLLKNSQYYIVAVDDLSTGDIK
                     KIPQASADVLKFIRCNVNDYESISAVMHSYKFDYVFHYAAMVGVLRTQQNPLKVFQDI
                     DGIRHICKLAVNTGVKRVFFSSSSEIYGEPVEIPQHVYTTPLNSRVPYAIVKNVGEAY
                     LRSYNQEFGLEYSIFRFFNTYGPKQSMDFVMSKFLIKALKNQDIELYGDGLQTRTFCY
                     VQDNVDACVNAFEKDLLINDVANIGGNKEITIKELAEIIVSKTNSKSRIVHVPPLKDG
                     DMLRRCPDNTYMREHLLTRPLTTLEDGISKLLEIGLFDLKR"
     gene            1202..>1690
                     /locus_tag="P5243_09190"
     CDS             1202..>1690
                     /locus_tag="P5243_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRS19668.1"
                     /translation="MQSVAPFQEIINTEIHRRLALPQFQEPKGLYAPCEYILKNGGKR
                     VRASLVLMAAKMFCHDYHKALPVALAFETFHNFTLIHDDIMDNADVRRGQPTVHVKWN
                     TNTAILSGDAVMIMAYSFFEDAKDFVPSLFSLLNKTALEVCEGQQYDIDLELADLHEP
                     WVT"
BASE COUNT          549 a          237 c          317 g          587 t
ORIGIN      
        1 tgtattattt tttggatttg gaatttttac attttctcag tttggaggaa cagtggcttt
       61 aggagcttta gtttctgtta ctctgcttat ggcaatgttt tcaaatgtaa tcttactgcc
      121 tgccttgtta ttaacattgg aaaaagctat taccaatcgt catttcaaag aaccattatt
      181 acatatatat aacgaagaag aagatataga tatagaagaa ctcaaaataa tttctaagta
      241 tacgaatgaa atctaaaatt aaaattcttg ttaccggagg tgccggattt attggaagtt
      301 ctttgattga aagacttttg aaaaattcac aatattatat agttgctgtt gatgatttat
      361 caaccggtga cataaaaaaa ataccgcaag cttctgcaga tgtgcttaaa tttattcgtt
      421 gcaatgtgaa tgattatgaa agtatttctg ctgtaatgca ttcttataag tttgactatg
      481 tgtttcatta tgcagctatg gttggggtat taagaacaca gcaaaatcct ttgaaagttt
      541 ttcaagatat tgatggaatt cgtcatattt gtaaattagc agtaaatacg ggtgttaagc
      601 gagtgttttt ttcatcatct tctgaaatat acggtgaacc tgttgaaatt cctcaacacg
      661 tatatacaac tcctcttaat tcacgtgtac catatgcaat tgtaaaaaat gtaggtgagg
      721 cttatttacg ttcatataat caagaatttg gattggagta ttcaattttc agatttttta
      781 atacctatgg gcccaaacaa agcatggact ttgtgatgag taaatttttg ataaaagctc
      841 tcaaaaatca agatattgaa ttatatggcg atggtcttca aactcgtaca ttttgttatg
      901 tgcaagataa tgttgatgcc tgtgtgaatg cttttgaaaa agatcttttg ataaatgatg
      961 ttgctaatat tggggggaat aaagaaatta ctattaaaga attagcagaa attatagttt
     1021 caaaaacaaa ttcaaaatca agaatagttc atgtaccacc tcttaaagat ggtgatatgc
     1081 ttcgtcgttg tcctgataat acatatatgc gagagcattt actaacgcgt cctcttacta
     1141 cgctcgaaga tggtatatca aaattattgg aaataggctt atttgattta aaacggtaat
     1201 tatgcagagt gtagctccat ttcaagaaat tataaataca gaaattcatc gaagacttgc
     1261 tttgcctcaa tttcaagaac ccaagggatt atatgctcct tgtgaatata tattgaaaaa
     1321 tggaggcaaa cgtgtgcgtg catcgttggt gcttatggct gcaaaaatgt tttgccacga
     1381 ttatcacaaa gcgttaccgg ttgcacttgc ttttgaaaca tttcataatt ttacgcttat
     1441 tcatgatgat ataatggata atgctgatgt aagaagaggg cagcctacag ttcatgtaaa
     1501 atggaatact aatacagcca tattatcggg cgatgctgta atgattatgg cgtatagttt
     1561 ttttgaagat gctaaagatt ttgtgccttc attattttcg ttacttaaca aaactgcttt
     1621 agaagtgtgt gaaggacaac aatatgatat tgatttggaa ttagctgatt tgcacgaacc
     1681 gtgggttacc
//
LOCUS       DAOTOW010000556         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_55978_length_1686_cov_3.391784, whole genome shotgun sequence.
ACCESSION   DAOTOW010000556 DAOTOW010000000
VERSION     DAOTOW010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55978_length_1686_cov_3.391784"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..>1686)
                     /locus_tag="P5243_09195"
     CDS             complement(79..>1686)
                     /locus_tag="P5243_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRS19669.1"
                     /translation="LYMTYGGNENDVEYEHDTKSVIVLGSGAYRIGSSVEFDWCGVSA
                     IKTIKKQGYRSVMINYNPETVSTDYDECDRLYFDELSFERVMDIIDLETPKGVIVSTG
                     GQIPNNLAMKLHRQNVPILGTSPVSIDRAENRHKFSMMLDSLGIDQPVWKELTSIDDI
                     YAFVDNVGFPVLIRPSYVLSGAAMNVVSNRDELEHFLTLAAKVSKQYPVVVTEFLQEA
                     KEIEIDAVAKDGEIQIYAISEHVEFAGVHSGDATMVLPPQKLYFETIRRIKRIAKEIA
                     KELNISGPFNMQFLAKNNDVKVIECNLRASRSFPFVSKVFKHDFIETATCIMLGVPFT
                     KPEKSMFDLDYIGVKASQFSFSRLSKADPVLGVDMSSTGEVGCLGEDYHDAILKSMLS
                     VGYRIPQKNILISSGPIKSKVDLLSTCKLLVENGYVLHATGGSSRFLDENNIPNILVY
                     WPDEDKQPNSLELLREKKIDLVINIPKNLSKAELHRDYIIRRSAIDFNIPLLTNARLA
                     RAFVEAFCNKKQDDISILSWDEFKLNN"
BASE COUNT          464 a          344 c          297 g          581 t
ORIGIN      
        1 atatatacat atttttaact aattcaaaat aaaaaagccc gcttgtgcgg gcttttttat
       61 atgctttttt gtataatttt aattgtttag tttgaattcg tcccaactga ggattgatat
      121 atcatcttgt tttttgttgc aaaatgcttc aacaaacgcg cgtgccaatc gtgcattggt
      181 tagcagcgga atattgaaat ctattgcact tcggcgaata atgtagtcgc ggtgcaattc
      241 ggctttgctc aaattttttg gtatgtttat taccaaatct atttttttct cgcgcaacaa
      301 ctccaatgaa tttggttgtt tgtcttcgtc gggccaatat acaagtatgt ttggaatatt
      361 attttcgtcg aggaatcgcg acgaaccacc cgtagcatgc aatacatatc cgttttctac
      421 taacaatttg caggttgaca ataaatcaac tttggattta attggccccg aagaaatcaa
      481 tatgtttttc tgcggaatgc ggtagcctac cgaaagcatt gattttaata tagcatcgtg
      541 gtagtcttcg cccaagcaac caacttcacc tgttgacgac atatctacac ccaacaccgg
      601 gtcggctttt gacaatcggc tgaatgagaa ttgcgatgct tttactccta tataatcaag
      661 gtcaaacatt gatttttcgg gttttgtaaa cggtacacca agcataatgc aggttgcagt
      721 ttctataaaa tcatgtttaa atacttttga tacaaacggg aagcttcgcg atgcacgcag
      781 gttacattct ataactttaa catcgttgtt ttttgccaga aattgcatat taaacggacc
      841 tgaaatgttc aattcttttg caatttcttt tgcaattcgt ttgatgcgac gtatggtttc
      901 gaaatataat ttttgcggag gcaataccat agtagcatca cccgagtgaa cccctgcaaa
      961 ttctacgtgt tctgaaattg catatatttg aatttcgccg tcttttgcaa ctgcatcaat
     1021 ttctatttct tttgcttctt gtaaaaattc ggttactacc accggatatt gtttcgatac
     1081 ttttgctgca agtgttaaga aatgttctaa ttcgtcgcga ttgctaacca cattcatagc
     1141 agcacccgaa agcacgtatg acggacgaat caataccggg aacccaacat tgtcaacaaa
     1201 tgcatagata tcgtctatac ttgttagttc tttccacacc ggttggtcta tacctaaact
     1261 atcgagcatc atagagaatt tatgtctatt ttcggctctg tctatactta ccggcgatgt
     1321 tcccaaaata ggtacatttt gacggtgcaa cttcattgca agattgtttg gaatttgtcc
     1381 gccggttgat acaattacac ctttaggagt ctccaaatct atgatgtcca ttactcgttc
     1441 aaacgaaagt tcgtcaaaat atagtcggtc gcattcatcg tagtcggtgc ttactgtttc
     1501 ggggttataa tttatcatta ccgatctata cccttgtttt ttgatggttt taatagcact
     1561 tacaccacac caatcaaatt ccacactact accaattctg tatgctcccg aacctaatac
     1621 aattacagat tttgtatcat gttcatattc tacatcgttt tcgttgccgc cgtaagtcat
     1681 gtataa
//
LOCUS       DAOTOW010000557         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_56038_length_1685_cov_2.325153, whole genome shotgun sequence.
ACCESSION   DAOTOW010000557 DAOTOW010000000
VERSION     DAOTOW010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56038_length_1685_cov_2.325153"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="P5243_09200"
     CDS             <1..929
                     /locus_tag="P5243_09200"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HRS19670.1"
                     /translation="LLGGSDLGGKAGELLIVINDEYKNSQTSESITEIMNQYELGLTQ
                     SEPPFSILMISRNHFSSVFRSHRNILFIDIHKKFTSPIVQFKKNVWTKQQNYIGIGVD
                     SPEQLLELLKLRHDNIIDFFVQAEIERYKLTYQNMPGNKVQNAVQQHMNIRIRIPEGY
                     EINKQAEGFMWISYESNEHSQGILIYDRPYKDTMQLEKWKLLQVRDSITKTHIPGPSK
                     GSYMTTEYIVPIQYKVGRYVNNDYTVELRGKWRVEHDFMGGPFVSYSFVDNTKKRLIT
                     IEGYVYYPNKSKRNFVRQLQAICQSVQFIENK"
     gene            937..>1685
                     /locus_tag="P5243_09205"
     CDS             937..>1685
                     /locus_tag="P5243_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRS19671.1"
                     /translation="MKILYYFSLIKFSHTIFALPFALIGYVLAIQTQSVSFSVYTLGY
                     VIACMIFARSAAMAFNRYSDYTYDSKNARTANREIPSGKISPSSALIFTIIHSVLFIV
                     TTWFINPICFYLSPVALLVILGYSYTKRFTYICHFILGIGLSLAPIGAYLAVTGYFSL
                     TPILFSCIVFTWTAGFDILYALQDEEFDRSETLFSIPALFGRTKAMRISIGTHIITAI
                     LVVITGLHVQLNYIYWIGASIFIGLLAFQHTL"
BASE COUNT          583 a          265 c          278 g          559 t
ORIGIN      
        1 gattattagg agggagtgat ttaggtggaa aagcaggtga acttcttatt gtaataaatg
       61 atgaatataa aaattcacaa acaagtgaat ctattacaga aattatgaat caatacgaat
      121 tggggttaac acaatctgaa ccgccattta gtattttaat gataagcaga aatcattttt
      181 caagcgtatt tcgttctcat agaaatattt tatttattga catacataaa aaatttacat
      241 cgcctatagt tcaattcaaa aaaaatgtat ggaccaaaca acaaaactat ataggtatag
      301 gagtagattc tcccgaacaa ttattggaat tattaaaact tcgtcatgac aatattatag
      361 atttttttgt gcaggcagaa attgaacgct ataaactaac ctatcaaaac atgcccggca
      421 acaaagttca aaatgcggta caacaacata tgaacataag aatacgtatt cccgaaggat
      481 atgaaataaa taaacaagca gaaggattta tgtggatttc ttacgaatct aacgaacata
      541 gtcaaggtat tttgatatat gaccgcccct ataaagatac tatgcaatta gaaaaatgga
      601 aattattaca agtacgagat tctattacaa aaactcacat acccggtccc agtaaaggta
      661 gttatatgac aaccgaatat attgttccta ttcaatacaa agtaggtcgc tatgtaaaca
      721 atgattatac tgttgagctt agaggcaaat ggcgtgtaga acatgatttt atgggtggtc
      781 cgtttgtgag ctattcattt gttgataata caaaaaaacg acttataact attgaaggat
      841 atgtatatta tcctaataaa agtaaacgta atttcgtgcg acaattgcaa gcaatatgtc
      901 aatcggtaca gtttattgaa aacaaataaa tgctcaatga agattctgta ttatttttct
      961 ttaattaaat ttagccatac catttttgcg ttgccatttg ctttaatagg ctacgtccta
     1021 gctattcaaa cacaatctgt atcattttct gtatatacat tgggctatgt aattgcatgt
     1081 atgatttttg ctcgttcggc agcaatggct ttcaacagat acagcgacta tacatatgat
     1141 agtaaaaatg ctcgcacagc aaacagagaa ataccaagcg gaaaaatttc accgtcttcg
     1201 gctcttattt ttacaattat acattcagta ttgtttatag taaccacatg gtttattaac
     1261 cccatttgtt tttatttgtc acctgtggca ctgcttgtaa ttttaggata ttcatatacc
     1321 aaacggttta cgtatatatg tcattttata ctgggaattg gattgtcact tgcacctatt
     1381 ggtgcatatt tggctgttac aggatatttt tcacttactc ctatcctatt ttcatgtata
     1441 gtatttacat ggactgcagg atttgatata ctttatgcat tacaagacga agaatttgac
     1501 cgtagcgaaa cattatttag tattccggca ctgtttggac gtacaaaagc aatgcgaata
     1561 tcaataggca cacatataat tacggcaata cttgttgtta ttacaggttt acacgttcaa
     1621 ttgaattaca tatattggat tggagcaagt atttttatag gattacttgc ttttcaacat
     1681 actct
//
LOCUS       DAOTOW010000558         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_56198_length_1681_cov_2.370849, whole genome shotgun sequence.
ACCESSION   DAOTOW010000558 DAOTOW010000000
VERSION     DAOTOW010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56198_length_1681_cov_2.370849"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..875
                     /locus_tag="P5243_09210"
                     /pseudo
     CDS             1..875
                     /locus_tag="P5243_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(901..>1681)
                     /locus_tag="P5243_09215"
     CDS             complement(901..>1681)
                     /locus_tag="P5243_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRS19672.1"
                     /translation="KEPEEIALIEQACNITTNAFKQTVPYIKPGIYEYEIEALLTKEF
                     IAHGASGHAFEPIIASGANSCFLHYVSNNTVLQPNTIVLMDFGAEYANYASDATRVIP
                     VSGKFTQRQKQVYEAVLRVLKQTTKLIITGTTILEYQEQTKLYIQEELLHLGLIKQHD
                     ITKQTKDKPAYFTYFMHGVSHFIGLDTHDVGNKDTVLEPGMVVSCEPGIYIANEGIGI
                     RLENDILITETGNKNLLEQMPIEIEEIEDLMNSYWHNKKIN"
BASE COUNT          529 a          264 c          306 g          582 t
ORIGIN      
        1 cagtaaaaac tattgcgcct acatttggag gtataaattt agaagatatt aaagctccgg
       61 aatgttttga aatagaacga agacttaagc aagaacttga tattcctgta atgcacgatg
      121 atcaacatgg tacagctata gtatcggcag ctgcattgct taatgcatgt gaaattgccg
      181 gaaaacaaat atctgaggtt agagttgtaa taaatggtgc aggagctgct gcaatttcat
      241 gtgctctttt gtatatttca gtaggtgttc aaaaagagaa tatcataatg cttgatagta
      301 aaggtgtaat tcatacaaat cgtaaagatt tagatatttc taaacaacaa tttgcacaga
      361 atgttccttt tacttcgctt gaatctgcca tgaaaggttc tgatgtattt attggtttgt
      421 caaaaggtga tattgtaacc gaacaaatgg tacaaagtat ggctccaaat cctattgttt
      481 ttgctttggc taatccaaat cccgagattt catatgataa agctatggca tctcgaaatg
      541 atataatttt tgcaaccgga cgaagtgata ttccaaatca agtaaataat gttttgggat
      601 ttccgtatat ttttagaggt gctttagatg tgcgtgcaac acaaataaat gaagaaatga
      661 aaattgcggc ggcatatgca atagctcaat taggaaaaga agaagtgccg gagagagtta
      721 aacaaacgta tggggataat atatcttttg gcgctcaata cataattcca aaaccaaatg
      781 atcctcgatt acgtgaatat gtatcgtatg cggtagctaa agcagctgtt gattcaggag
      841 tagctcgcaa tccaattaca aattgggaag aatacagaaa agtattacgt acaaaaagtt
      901 ttaattaatt tttttattat gccaatagga attcattaaa tcttcgattt cttcaatttc
      961 tattggcatt tgttccaata agtttttatt accggtttca gttattaaaa tatcattttc
     1021 taatcgtatt cctattcctt cattagctat atatattccc ggttcgcatg acactaccat
     1081 acccggttct aaaacggtat ctttgtttcc tacatcatgc gtgtctaacc caataaaatg
     1141 tgaaacacca tgcataaaat aggtgaaata tgcaggttta tctttggttt gtttagtaat
     1201 atcatgttgt ttaatcaacc caagatgcaa aagttcttct tgaatatata gttttgtttg
     1261 ttcttggtat tcaagaatcg ttgtacccgt aataatgagt ttagttgttt gtttcaatac
     1321 tcgcaataca gcctcatata cttgtttttg tctttgtgta aatttgcctg atacaggtat
     1381 tacacgagtt gcatcacttg cataatttgc atattctgct ccaaagtcca ttaaaactat
     1441 ggtgttaggt tgtaatactg tattgtttga tacataatgt aaaaaacagg aatttgctcc
     1501 cgatgctata ataggctcaa atgcatgccc cgatgctcca tgggctataa attctttggt
     1561 aagtaaagct tcaatttcgt attcatatat gccgggttta atatatggta ctgtttgttt
     1621 aaatgcattg gttgtaatat tacatgcttg ttcaattaat gcaatctcct cgggttcttt
     1681 t
//
LOCUS       DAOTOW010000559         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_56248_length_1680_cov_2.754462, whole genome shotgun sequence.
ACCESSION   DAOTOW010000559 DAOTOW010000000
VERSION     DAOTOW010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56248_length_1680_cov_2.754462"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..1040
                     /locus_tag="P5243_09220"
     CDS             552..1040
                     /locus_tag="P5243_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19673.1"
                     /translation="MKKIVTIVTVIMSLSVSTFGQVNPHAIGLRLGGDGNVNGAEFSY
                     QHGMGEANRLEFDLGFRGNKNYNSMYVSGVYQWDWNLTSGLNWYVGPGAALGFYNWDD
                     GNTSSSGLHLGLGGQIGLEFDFNAIGAPILLSIDARPMFDFIGYDNGLGWGTALGIRY
                     TW"
BASE COUNT          611 a          169 c          304 g          596 t
ORIGIN      
        1 agtaggtata tcaaacggta agtttatggg aaatcccgaa gatactgtaa agtctgtaga
       61 atatgaaata ggaaaggtta acaaattcat tttcttgcaa gaaaaaaata ataatgatat
      121 gaaaactata acagaatata tatatgtttt tttcatagta tgagatttag aacattatat
      181 tgtactagta aaattaatac gataaatcaa aaatacaaaa tgtatgtgtt ttatattata
      241 agcatacaat gaaaatttgg ataataaaaa taatgaaaat tatatcaaaa aaagataatt
      301 aacctgtcat aataatagcg agtattatag tttacaggtt tgtgtatgta aagaaaaaag
      361 gagtatattt gaattgtgaa acggcttata taagtctata ctttgtatct gagttgttac
      421 aatgtataac tatttaaaaa ataggaaatt attagaaata ctttaatata catgtggacg
      481 cttgaaacca ctcaaaaaaa cagggcgtaa ccgaaaagcc atacgagtag ggaaacttaa
      541 aataaaaaaa aatgaaaaaa atagtaacaa ttgtaacagt aattatgagt ttgagtgtat
      601 caacttttgg gcaagtaaat ccccatgcaa ttggacttcg tttaggtggt gatggaaatg
      661 taaacggagc agaattctca tatcaacatg gcatgggcga agcaaatcgt ctggaatttg
      721 atttaggatt tagaggaaac aaaaattata atagtatgta tgtttccggt gtgtatcaat
      781 gggattggaa tttaacaagt ggtttaaatt ggtatgttgg tccgggtgca gcattaggtt
      841 tttacaattg ggatgatggg aatacatcga gtagtggttt gcatcttgga ttaggtggtc
      901 aaattggatt agagtttgat tttaatgcaa ttggtgctcc aattttatta agtatagatg
      961 ctcggcctat gtttgatttt attgggtatg acaatggctt aggatgggga accgctcttg
     1021 gtataagata tacttggtaa tagtataccg tattttacgg tacttttttt tcatggtagt
     1081 aagtttgtga ggacgttcgt tcttgatagt ttgtttaagt tgctatcggt atatggaatg
     1141 aagccaacaa aatactacca tgaatgtttt atgagagaat gttgttgttt cttttttttc
     1201 ttaatgttat ataaaagtta cttacctata tcttaatagc tacacgtata atacatggta
     1261 ttaacaatat tcagcgttct gaacttgaac tcaaaattac gttgaactga cgttaaaaca
     1321 gcttgtttgt tatttgaaaa ggataataaa gaacataaac taaaggattt tgagagaaat
     1381 attggaaatt atggctttgt attcatttgt aatcaatatg aacttgattg ttttgtttac
     1441 ttattttttt atatttacag aattgcaagg attattagat catatgcaat aaaagcaatt
     1501 ttgtaagcag tgattatata caatattgat ttgtaggaac cgataattca ataagttttt
     1561 ttacagtgta ttttttttac aaattgctat gaataatttt tttttaataa tactaatctt
     1621 attatcaata gaattatatt ctcaaaatga tactttacac aatattaatg aaaatttaaa
//
LOCUS       DAOTOW010000560         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_56370_length_1678_cov_2.470733, whole genome shotgun sequence.
ACCESSION   DAOTOW010000560 DAOTOW010000000
VERSION     DAOTOW010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56370_length_1678_cov_2.470733"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="P5243_09225"
     CDS             <1..341
                     /locus_tag="P5243_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRS19674.1"
                     /translation="NKSEHGHKISLHIGDCLQLIPQFPYMFDVVFIDGNKRHYIDYYE
                     AAMSKLNSRGFIFVDNTLWDGHVIDKHIEATDFQTQGIIAFNTHVKQDTRVETAIFPI
                     RDGITVLYKK"
     gene            348..1577
                     /locus_tag="P5243_09230"
     CDS             348..1577
                     /locus_tag="P5243_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRS19675.1"
                     /translation="MMYKTIIVCGVFICSVYSSYSQAFPFPQNKQYPYGYISKAITPA
                     YIQKNYEYWKQHAVYMCNTQEARIMKFPNDNQTTVSEGIAYGMLITAYMGDKQLFDNL
                     LNYYSARTNCRGMMNWIYKDCETGDNKKNGATDADIDIAFALLVAAKQWPQDTRYKQK
                     ASMVIDSLQKYCFVVCNGEIVQKPGDDFGGCYCTNPSYYSPGYYKIFGLFKKQEGNLN
                     AYSFWEKAYFDVYSTLLKNAHPITGLVYAWTNFEGKDPQNCYYEVTGSGTYNSYQYDA
                     CRTPWRITMDYVWFGNEQARDYLQRISRFVQAPIYAQYDSKGTIWYGAGGIQNIVDSY
                     WTNGLRRINPDYADWGHRHAIAFVGSFALASMATSQSNVDICMNELSTLQALRYYESS
                     LGLLYSLLASGNFWNPL"
BASE COUNT          591 a          264 c          287 g          536 t
ORIGIN      
        1 ttaataaatc agaacatggt cataaaattt cattacatat aggagactgt ttacaactta
       61 ttcctcaatt tccatatatg tttgatgtag tttttattga tggaaacaaa cgtcattata
      121 ttgattatta tgaagctgct atgagtaaac ttaattcgag aggatttatc tttgttgaca
      181 acacgttgtg ggacggtcat gtaattgata aacacataga agctactgat tttcaaacac
      241 aaggcattat tgcatttaat actcatgtaa aacaagatac acgtgtagaa actgcaatat
      301 tccctatacg cgacggtatt actgtattat ataaaaaata aaaaagtatg atgtataaaa
      361 caattattgt atgtggtgtt tttatttgtt ctgtttatag ttcatactca caagcatttc
      421 cgtttccaca aaacaaacaa tatccctatg gttatataag taaagccata accccggctt
      481 acattcaaaa aaactatgaa tattggaaac aacatgctgt atatatgtgt aatactcaag
      541 aagcacgtat catgaaattt cccaacgaca atcaaacaac agtttcagaa ggtatagcat
      601 acggtatgct tattacagcg tatatgggcg acaaacaatt atttgataat ttattgaatt
      661 actattctgc acgaaccaac tgcagaggaa tgatgaattg gatatacaaa gattgtgaaa
      721 caggagacaa caaaaaaaat ggtgcaaccg atgccgatat tgatatagca tttgcattgc
      781 ttgtagctgc taaacaatgg ccgcaagaca ctcgatataa acaaaaagca tctatggtta
      841 tagacagttt acaaaagtat tgttttgttg tttgtaacgg agaaatagtt caaaaaccgg
      901 gagatgattt tggcggttgc tattgtacca acccatcata ttattcacca gggtattaca
      961 aaatttttgg tttattcaaa aaacaagaag ggaatttgaa tgcttactca ttttgggaaa
     1021 aagcatattt tgatgtttac agtacattat taaaaaatgc acatcctatc acaggtttag
     1081 tgtatgcatg gactaacttt gaaggcaaag atcctcaaaa ttgctattat gaagttacgg
     1141 gttcgggtac atataattca taccaatatg atgcttgccg cactccttgg cgaatcacaa
     1201 tggattatgt atggtttggg aatgaacaag ctcgtgatta tttacaacgt atttctcgtt
     1261 ttgttcaagc tcccatatat gcacaatatg atagcaaagg aacaatttgg tacggagcag
     1321 gaggcataca aaatattgtt gacagctatt ggacaaatgg actaagaaga ataaaccctg
     1381 actacgcaga ttggggacat agacatgcta ttgcatttgt tggttcgttt gctttagcat
     1441 ctatggcaac cagccaatca aatgttgata tttgcatgaa tgaattaagt accctgcaag
     1501 cactccgata ttatgaatca agcttaggat tactatactc actattagca agcggaaatt
     1561 tttggaatcc tttataacag tataagaata aaattgcttt taccaattta acgtttctat
     1621 atacacatta tattaaaaaa acatttatat aaaattgttt atttctacct tttattta
//
LOCUS       DAOTOW010000561         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_56449_length_1676_cov_3.293029, whole genome shotgun sequence.
ACCESSION   DAOTOW010000561 DAOTOW010000000
VERSION     DAOTOW010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56449_length_1676_cov_3.293029"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="P5243_09235"
     CDS             <1..1610
                     /locus_tag="P5243_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRS19676.1"
                     /translation="FISGWLRKSNVTTGAEWIATRFGNGKSSKLSHGIVVVFALIIGL
                     SMLSYGFVGLGKFLMLFAPWEYVSSLLSIPYNAIAIEYVPHVYGIVFTLFATFYAILG
                     GMVSIVWADILQYTIMTVASITIGIIAMNMVSPETLHALIPQGWDSPFFGTHLNIDWS
                     GKIAEVNQKIQEDGFSLFSLFFGFMFMKGVLSSLAGPAPTYDMQKILANRTPQDALKM
                     SGSVSVILNPVRYFMITGFAILAIAYYQDLNLLVGNKIDFEQILPSAMIQFLPVGLLG
                     LLLAGLLAAFMSTFAGTLNAAQAYVSNDIYLKYINPNASQKQIKKTNYTVGIAVVLIS
                     TLIGFTLKSINEILLIVTSAFYASYIASNVLKWYWWRFNGDGYFWGMITGIVSGLIIG
                     VIQIKYPHSITNIIPGMPEGLIALYLFPVTLIISLIGCLAGTLLTAPTDSQTIETFYT
                     TVKPWGFWNPVFKSVKSKQPDFTKNTRFLKDMGNVAIGIIVQTCLVAMPIFIIIRNWQ
                     SLFITVSIIAIGAIILKYTWWDKLHEI"
BASE COUNT          535 a          264 c          295 g          582 t
ORIGIN      
        1 tgtttatttc gggttggtta cgaaaatcaa atgtaacaac cggagccgaa tggatagcaa
       61 cacgatttgg aaatggcaaa agcagtaaac tttctcacgg aatagttgta gtatttgctc
      121 ttattatagg tctttcaatg ctttcgtacg gatttgtagg attaggtaaa tttttaatgc
      181 tttttgctcc ttgggaatac gttagctctt tactttcaat tccatacaat gctattgcaa
      241 tagaatatgt gccacacgtg tatggcatag tatttacctt atttgcaaca ttttatgcta
      301 ttttaggagg aatggtaagt atagtatggg cagatatttt gcaatatacc attatgacag
      361 tagcatctat tactattggt ataattgcta tgaacatggt aagtcctgaa acactccatg
      421 ccctaattcc acaagggtgg gattcaccat tttttggaac acatttgaat atagattggt
      481 ccggaaaaat agccgaagta aatcaaaaaa tacaagaaga tgggttctct ttatttagtt
      541 tattttttgg atttatgttt atgaaagggg ttttaagcag tttggccggc ccggcaccaa
      601 catacgacat gcaaaaaatt cttgccaacc gaactccgca agatgcatta aaaatgagcg
      661 gttcagtttc ggttatactc aatcctgtaa gatattttat gattacaggt tttgctatat
      721 tggcaattgc atattatcaa gatttgaatt tactggtagg aaacaaaatt gattttgaac
      781 aaattttgcc ttcggcaatg attcaatttt tacctgttgg tttactgggt ttgttacttg
      841 ccggtcttct tgcagcattt atgtcaacat ttgcgggtac actcaatgct gctcaagcat
      901 atgtatcaaa cgatatttat ctgaaatata taaaccccaa tgcttcacaa aaacaaataa
      961 aaaaaactaa ttacacagta ggaattgcag tggtattaat aagcacactt ataggattta
     1021 cacttaaaag tataaatgaa atactgctca tagtaacaag tgcgttttat gcaagctata
     1081 ttgcatctaa cgttcttaaa tggtactggt ggcgcttcaa cggcgatgga tatttctggg
     1141 gaatgattac cggtattgtt tccggtctaa ttataggagt aatacaaatt aaatatcctc
     1201 atagtattac caatataata ccgggtatgc ccgaaggatt aattgcatta tatttatttc
     1261 cggtaacact tattatttca cttataggtt gtttagcagg tactttactt actgctccta
     1321 ccgattctca aactatagaa actttttata caaccgtaaa accatggggt ttttggaacc
     1381 ctgtttttaa atctgtaaag tcaaaacaac ctgattttac aaaaaatact cgtttcttaa
     1441 aagatatggg aaatgttgca attggaatta tagttcaaac atgcttagtt gccatgccta
     1501 tttttataat tattcgcaat tggcagagtt tatttattac agtatcaatt attgcaatag
     1561 gagctataat tttaaaatat acatggtggg ataaattaca cgaaatttaa aaacatgaaa
     1621 actgtttcaa aaagtatata tttcatatac gttattattt ttgcttttac ctcgtg
//
LOCUS       DAOTOW010000562         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_56629_length_1673_cov_2.595797, whole genome shotgun sequence.
ACCESSION   DAOTOW010000562 DAOTOW010000000
VERSION     DAOTOW010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56629_length_1673_cov_2.595797"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="lgt"
                     /locus_tag="P5243_09240"
     CDS             complement(<1..799)
                     /gene="lgt"
                     /locus_tag="P5243_09240"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRS19677.1"
                     /translation="MIFSQVIWNVSPELVAFTDSYAIRWYGVLFSLGYIVAYFILLQA
                     FKKEKIPQIVLEKLTLAAIVGGVVGARLGHCFFYEPMRYIHNPIEILYVWHGGLASHG
                     GAIGVLIAFWFVIRKMEYSYLFLLSRAMLVVPLTGAFIRIGNLMNSEIYGTETSLPWG
                     FVFQRSSDVIAGIEQAVPRHPTQLYEAGAYILVFIGLQIYYWLNLYKQKTISTNTIIG
                     VFLVGIFASRFIIEFFKVNQAAFESAWYLNLGQLLSIPFILYGVYSLF"
     gene            complement(796..>1673)
                     /locus_tag="P5243_09245"
     CDS             complement(796..>1673)
                     /locus_tag="P5243_09245"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRS19678.1"
                     /translation="ELDSFTFMTFILLIVGLRLGHCLFYEPDEYLKNPIRILFVWEGG
                     LASHGGAIGLLLSFYLFTRKSTKSFVWIASRAAVVIPFTAACVRLGNLMNSEIYGVAT
                     HLPWGFVYLRDAVIFTTPESLADILLQSKLIATNMQEQLHQFLMLNAQVFTKGIPYTE
                     LQHSLVQAGFVAQEKIDVLPHILLQAQLLHPSHPTQIYEALVYLSIFGILMWYYFSRS
                     AKQQVISSYLILGIAFIGIFGSRFCIEFIKNNQVAFEQGMQLNMGQWLSIPFILAGVF
                     FIYVYTKQRKTNTIS"
BASE COUNT          631 a          325 c          238 g          479 t
ORIGIN      
        1 taaacagcga atacactcca tatagaataa acgggatact taataattgg cccaaattta
       61 aataccacgc cgattcaaaa gcagcttgat ttactttaaa aaactctata ataaatctac
      121 ttgcaaagat tcctactaag aatactccta ttatagtatt agtagaaata gttttttgtt
      181 tatacaaatt caaccaataa taaatctgta atccaataaa aaccaaaata taagcccctg
      241 cttcatacaa ttgtgtagga tgacgaggaa ctgcctgttc tatacccgca ataacatcag
      301 aacttcgttg aaatacaaat ccccacggta aagaagtttc agtaccataa atttctgaat
      361 tcataagatt tcctatacga ataaacgcac cggtaagagg aactacaagc attgctctgc
      421 tgagaagaaa caaatatgaa tattccatct ttcgaataac gaaccagaat gcaataagaa
      481 cacctattgc acctccatga cttgccaatc cgccatgcca tacatacaat atttctatag
      541 gattatgtat atatcgcata ggttcgtaga aaaagcaatg ccccaatcgg gcacctacta
      601 caccaccaac tatagctgca agtgttaatt tttctaatac tatttgcggt attttttctt
      661 tcttaaatgc ttgcagtaaa ataaaatagg ctactatata ccccaaagaa aataatacac
      721 cataccatcg tatagcatat gaatcggtaa atgcaaccaa ttcgggcgac acattccata
      781 ttacctgact aaatatcacg atatagtatt tgtttttctt tgtttagtgt atacatatat
      841 aaaaaatact cctgccaata taaaaggtat acttaaccac tgccccatat tcaactgcat
      901 accttgttca aatgcaactt ggttattttt aataaattca atacaaaaac gcgagccaaa
      961 aattccaata aaagcaatac ctaaaataag atacgatgat attacttgtt gcttagcact
     1021 acgcgaaaaa taataccaca tgagtattcc aaaaatagaa agatacacca aggcttcata
     1081 aatttgagta ggatgacttg ggtgaagtaa ttgagcttgt aataaaatat gcggtaatac
     1141 atcaattttt tcttgagcaa caaaaccggc ctgcactaat gaatgttgta attctgtata
     1201 tggtattcct ttagtaaata cttgagcatt gagcattaaa aattggtgta attgttcttg
     1261 catattagta gcaataagtt ttgattgcaa caaaatatcg gcaagtgatt cgggagttgt
     1321 aaaaattacc gcatcacgaa gataaacaaa tccccaaggc aagtgagttg caacgccgta
     1381 aatttcagaa ttcattaaat tgcccaatcg aacacatgcg gcggtaaaag gaattacaac
     1441 agcagcacgc gatgctatcc acacaaatga ttttgtggat ttccgagtaa acaaataaaa
     1501 tgaaagcaat aaacctatag cacctccatg acttgccaat cctccctccc atacaaataa
     1561 aattcgtata ggatttttta aatattcatc aggttcataa aacaaacaat gccctaaacg
     1621 taaaccaact ataagcaata taaatgtcat aaatgtaaaa ctgtctaatt ctt
//
LOCUS       DAOTOW010000563         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_56768_length_1670_cov_2.847059, whole genome shotgun sequence.
ACCESSION   DAOTOW010000563 DAOTOW010000000
VERSION     DAOTOW010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56768_length_1670_cov_2.847059"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1670
                     /locus_tag="P5243_09250"
     CDS             <1..>1670
                     /locus_tag="P5243_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRS19679.1"
                     /translation="LETSDVGIANQMEKISNPSVQPGLYYVVVILKQGQGMYSLKLDY
                     TQEQPTNPVLSNLFIDDFEDEDLVSLQGNEWGVITDNSSGGNSSANIYLNEYGLNNSI
                     GALRVQYFLDKASLPYDPFILARINLQANAQPVNVSAAQGIRLDIENPEIVKIQLVTA
                     NVTDYAYYEYVVNKNIIPSNLVVPFSSFTQPVWGSPAQLDLTQLVAIQIQTVNTDGTE
                     SYFVIDNIEIYANQTQPSGFVLDDFSDGDLINNLGGGWYKYEDSQMGGGSVLQYSLLP
                     ENTLQATYTLEQGSLNFDPFVLVGTMLNGQNTSVDIIGSTGITFTYQGRDFFFDVIDD
                     IGMAGENYQIHIPGATEPKTVTILWSQLNQPSWATNKYPLNLSIIKSFQWRIQAPSGT
                     SGVINIDDVTILNYQTNQSQDSVDVTFSVNMKNQTVSPHGVRLNGSFINWETSQAIPM
                     QYVGNDVYTTTVKLPVGAVIQYKFINGTGDDYAAYEVINQGCADPMYGNRVLTVSSTN
                     KVLPLVCFASCTDCIESGGVNVTIMTNQNVGSAPLLVEFTGMSLSNII"
BASE COUNT          551 a          225 c          316 g          578 t
ORIGIN      
        1 tacttgaaac atctgatgtt ggtatagcaa atcaaatgga gaaaatttca aatccgtctg
       61 ttcaacctgg gttgtattat gtagttgtaa tattaaaaca aggtcaagga atgtattctc
      121 ttaaacttga ttatacgcaa gaacaaccaa caaatcctgt tttaagtaat ttatttattg
      181 atgattttga agatgaggat ttggtttcat tgcaagggaa tgagtggggg gtaattacgg
      241 ataattcttc aggtggaaat tcatcggcaa atatttattt gaatgaatat ggtttaaata
      301 atagtattgg tgctttacgt gttcagtatt ttttagataa agcatcactg ccttacgatc
      361 cttttatatt agcacgtatt aatttacaag caaatgctca gcctgtaaat gtaagtgcag
      421 ctcaaggtat tcgattagat atagaaaatc cggaaatagt aaaaattcaa ttagtaacag
      481 caaatgttac agattatgca tattatgaat acgttgtaaa taagaatatc ataccaagta
      541 atcttgttgt gccattctct tcatttactc aacctgtttg gggttcacct gctcaattag
      601 atttaactca attggtagct attcaaatac agactgtaaa tactgatgga actgaaagtt
      661 attttgtaat tgataatatt gaaatttacg caaatcaaac acaaccttca ggttttgtat
      721 tagatgattt ttctgatggt gatttaataa ataatttagg aggtggttgg tataagtatg
      781 aagattctca aatgggagga ggctccgttc tgcaatattc tctattgcct gaaaatacat
      841 tacaagctac ttatacatta gaacaagggt ctttgaattt tgatccgttt gttttggttg
      901 gaactatgtt gaatggtcaa aatacttctg ttgatataat cggatctaca ggaattactt
      961 ttacatatca aggtagagat ttcttttttg atgtaattga tgatattggt atggccggtg
     1021 aaaattatca aatacatata cccggtgcta ctgaaccaaa aactgtaacc atactttgga
     1081 gtcaattaaa tcaaccgagt tgggctacta ataaatatcc gcttaattta tctatcataa
     1141 agagttttca atggcgtatt caagctccat caggaacaag tggagtaata aatatagatg
     1201 atgttaccat tttaaattat caaacaaatc agtcacaaga ttcggttgat gttacttttt
     1261 ctgtgaatat gaaaaatcaa accgtttctc ctcatggtgt acgtttgaat ggtagtttta
     1321 taaattggga aacaagtcaa gctattccta tgcaatatgt aggaaacgat gtgtatacta
     1381 caacggtaaa attaccggta ggagcagtaa tacaatataa atttataaat ggtacgggtg
     1441 atgactatgc tgcgtatgaa gttataaacc aaggatgtgc tgatcctatg tatggtaata
     1501 gagttcttac ggtaagttct acaaataaag tactgccttt ggtatgtttt gcttcatgta
     1561 ctgattgtat tgagtcgggt ggagttaatg taacaattat gacaaatcaa aatgttggta
     1621 gtgcaccatt gttagttgaa tttaccggta tgtctttaag taatattatt
//
LOCUS       DAOTOW010000564         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57052_length_1664_cov_3.200124, whole genome shotgun sequence.
ACCESSION   DAOTOW010000564 DAOTOW010000000
VERSION     DAOTOW010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57052_length_1664_cov_3.200124"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="P5243_09255"
     CDS             <1..1577
                     /locus_tag="P5243_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19680.1"
                     /translation="KLTWNKNTYNTFACNVYYGDDYLNYWGKQGLSESFRYTNAWGNK
                     LVSLTWNNTRFTKLHASQIVSYTNYRSATKLKFTIHDSSTVNIRMNDMSSKSQALAQS
                     LWEYTPTSWWRVQFGLQSTFDTYIPDKRYNSLEQQSEPHTSLYGNETALFADNVFRIA
                     QCISFTPGVRVQKNIVESYTSTHIEPRIQLAYKISPYHTISAHYMQVYQNSHMLFTNG
                     SIMSNEIWIPSDTDIAPSHATQYSINWNGQFLKSMFATECNVYYKEMENLITFKDGYT
                     SIRGDEQWKQKIATNGVGTSYGLELFIRKQKGDWQGFTGYSYSHTTRTFAQTNQGKTY
                     VFDYDRPHSFSIAILRKFSDAWSVSGTWVFQSGLPYTPVLGRQSAIGLNFNNEYVVYE
                     SLMYGERNSARMKPYHRLDLGITYTSTNKHNQRCEWNFSIYNVYNRLNPYYYYYNHDN
                     GSEFYTPNYQDEIGFKPLKMYQISFFPIIPTMSYKVYFEGERYRAYTQEKQKNKQDKG
                     PQTFKEKFRAWMFYEN"
BASE COUNT          593 a          320 c          278 g          473 t
ORIGIN      
        1 caaaacttac gtggaataaa aatacatata acacatttgc atgcaatgta tattatggcg
       61 acgattattt gaattattgg ggcaaacaag gactttcgga atcgtttcga tataccaatg
      121 catggggtaa taaattagta tcgcttacat ggaacaatac ccgtttcaca aaactacatg
      181 catcgcaaat tgtttcctat accaactaca ggtcggctac caaattaaaa tttacaatcc
      241 acgattccag cactgtgaat attcgcatga acgatatgtc ttcaaaatca caagccctgg
      301 cacaatcgct gtgggaatat actcctactt cgtggtggcg agttcaattt ggcttacaat
      361 cgacgttcga cacctacatt cccgacaaac gctataattc attggaacaa caaagtgaac
      421 cacacacttc gttgtatggt aacgaaacag ctctatttgc cgataatgta tttcgtatag
      481 cacaatgcat atcgtttact ccgggagtac gtgttcaaaa aaatattgta gaatcatata
      541 cttcaacaca tatagaacct cgcatacaac ttgcgtataa aatttcgccc tatcatacca
      601 taagcgctca ttacatgcag gtatatcaaa attcgcacat gctgtttacc aacgggtcta
      661 ttatgagcaa cgaaatatgg attccatcgg atacagacat tgcaccttcg catgccacac
      721 aatattctat aaattggaac gggcaatttt tgaaaagtat gtttgctacc gaatgcaatg
      781 tatattataa agaaatggaa aatttaataa catttaaaga cggatataca agcatacgcg
      841 gcgatgaaca atggaaacaa aaaattgcaa caaacggagt tggcacatca tatggcttgg
      901 aattattcat tcgcaagcaa aaaggcgatt ggcaaggatt taccggttat tcgtattctc
      961 ataccacacg tacatttgcc caaaccaacc aaggaaaaac atatgtattt gattacgacc
     1021 gacctcactc attctctata gcaatactgc gtaaattttc tgatgcatgg agtgtaagcg
     1081 gtacatgggt gtttcaatcg ggcttaccat acacaccggt tcttggcaga caatcagcta
     1141 ttggattaaa tttcaacaat gaatatgtag tgtatgaatc gcttatgtac ggtgaacgta
     1201 atagtgcccg catgaaacca tatcatcgat tagatttagg aatcacatat acttcaacca
     1261 ataaacataa ccaacgatgt gaatggaatt ttagcattta taacgtgtac aacagactaa
     1321 acccctatta ttactattac aatcatgata atggtagtga attttacaca ccaaattatc
     1381 aagacgaaat aggatttaaa cctttaaaaa tgtatcaaat tagctttttc cctatcattc
     1441 caaccatgtc gtacaaagta tatttcgaag gagaacgata tagagcctac acacaagaaa
     1501 aacaaaaaaa caaacaagac aaaggaccac aaacattcaa agaaaaattt agagcgtgga
     1561 tgttttatga gaattagctt tttagagaag agtataaata acttattagg aaaacaatgt
     1621 ttctatacgt tttcttgatt ttctaaaaat gtagtcttgt tttt
//
LOCUS       DAOTOW010000565         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57057_length_1664_cov_2.959602, whole genome shotgun sequence.
ACCESSION   DAOTOW010000565 DAOTOW010000000
VERSION     DAOTOW010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57057_length_1664_cov_2.959602"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="P5243_09260"
     CDS             <1..500
                     /locus_tag="P5243_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19681.1"
                     /translation="IDVVIVTATVTQTIALAQGWNLISFNVVPSNTTIASVFAGVMTQ
                     VNTIKNSDGFYKPGQDAELQSLTNISVGAAYLVHMKTAQTLSVSGTAPGIVTMPLKAG
                     WNMLGYPKSTNGTTTTVLGTTWTSAQIIKNFEAFLDKTSGTLTTMKPGEGYYIYMNTA
                     SNVTF"
     gene            574..1242
                     /locus_tag="P5243_09265"
     CDS             574..1242
                     /locus_tag="P5243_09265"
                     /inference="COORDINATES: protein motif:HMM:NF016209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HRS19682.1"
                     /translation="MNIEQIHTIQGATECLLFFNGWGMDTNAIQHITNRNYDIFACNN
                     YTDLTVDFSIIENYSKVHIVAWSMGVWACMNVQSKLPKTIASSIAINGTELPMHDTMG
                     IPSTIFKGTIEAWNDNTRTKFNIRMCGSREEWIQYKPYMSKRISTEQKEELEEIHSHI
                     LLNKNSTPFNWTSVVIGERDLIFTAENQKNYWQSHIKRFILPIPHYPFGNITTWDEIL
                     NWAK"
     gene            1383..1664
                     /locus_tag="P5243_09270"
                     /pseudo
     CDS             1383..1664
                     /locus_tag="P5243_09270"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
BASE COUNT          604 a          281 c          267 g          512 t
ORIGIN      
        1 ccatagatgt ggtgattgta actgcaactg ttacacaaac cattgcattg gctcaaggat
       61 ggaatttaat atcatttaat gtagttccat caaatacaac aatagcatca gtttttgcag
      121 gagtaatgac acaagtaaat actataaaaa atagtgacgg tttctacaaa ccgggacaag
      181 atgcagaatt gcaaagtctt acaaatattt cggtaggcgc agcttacttg gtacacatga
      241 aaacagcaca aacacttagc gtatcaggaa cagctccggg tatcgttacc atgcctctca
      301 aagcaggttg gaatatgctt ggctatccaa aatctaccaa tggtacgact acaacagtat
      361 taggaactac atggacaagt gctcaaataa ttaaaaactt tgaagcattt ttagataaaa
      421 catcgggtac acttacaaca atgaaaccgg gcgaaggata ttatatttat atgaataccg
      481 cttcaaatgt aactttctaa taaaaactac ttttttttaa tagtattcgg cggaagattt
      541 tcgtaatttt ccgccgattt atttttatcc aatatgaata ttgaacaaat acatactata
      601 caaggagcca ctgaatgttt actattcttt aatggatggg gcatggatac aaatgcaatt
      661 caacatatta ctaatcgcaa ttacgatata tttgcatgta ataattatac cgatttaaca
      721 gttgattttt caataattga aaattactca aaagttcata tagtagcatg gtctatgggt
      781 gtatgggctt gtatgaatgt acaatcaaaa ctacctaaaa ctattgcatc gagtattgca
      841 attaatggta cagaattacc aatgcacgat actatgggga ttccttcaac tatatttaaa
      901 ggtacaattg aggcatggaa tgataataca cgcactaaat ttaatatacg aatgtgtggt
      961 tcaagagaag aatggattca atataagcct tatatgtcta aacgaataag cactgaacaa
     1021 aaagaagaat tagaagaaat tcattcacat atattattaa acaaaaatag cacacccttt
     1081 aattggacat cagtcgtaat tggtgaaaga gatttaattt ttacagccga aaatcaaaaa
     1141 aactattggc aatcacatat aaaaagattt attttgccaa tccctcatta tccctttggg
     1201 aatattacaa catgggacga aattttaaat tgggcaaaat gaccttacct gacaaacaca
     1261 taattggtga gcgtttttgg aaaaataaag aaacgtataa acaataagca actgtacaaa
     1321 aagtcttatg tgaatgcttt gccggcactt tacaatcata ttcatttccc ctatgtaatg
     1381 tttttgaaat aggttgtggc acaggatttt taacacaaga attatgctct ataccctcta
     1441 ttcaaaaact atatgtgaat gatgtttcac caaaaatgca acaagacatt gatgctataa
     1501 tagctcaatc aaaaatatca gactatgttt tcattgcagg tgatgcagaa acaattgatt
     1561 ttccttcttc aatagatgct attgtttcta catctacact acagtggttt cattcaatct
     1621 cctcattttt tgataaagct gctctatcac ttccttcaca aggt
//
LOCUS       DAOTOW010000566         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57076_length_1664_cov_2.048477, whole genome shotgun sequence.
ACCESSION   DAOTOW010000566 DAOTOW010000000
VERSION     DAOTOW010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57076_length_1664_cov_2.048477"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1664)
                     /locus_tag="P5243_09275"
     CDS             complement(<1..>1664)
                     /locus_tag="P5243_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19683.1"
                     /translation="GKTYIIQWFTHAQNWNLDFIKDAVLPPGYTCQNPIVISQEQTVV
                     QPTGNITWLELSYPQSGTVSLSSNLNIEANVFLFDVCTPLSENLKSTVPYLMMDSAIV
                     FWPQANKKYKMLIVFSEQAPTMPYTIQSKYLSNQSQPANVVCADAKPLSLGAQQTTTV
                     YGNYWYSYNAKANTIVHLDFNNEQFQQLQIWPSIYFSCPSPFDSHGGGYYNNVFMPCG
                     NNLQGITMPIEKDTTLYIQFRSRNLSTTVNWTLNEYSTKSVAITSFSSMQQVSNAVIN
                     SITRTINLTVAQSASISETTIDFKVSPGATIKTSSGITLCPGSQLFFNNGTTTITVTS
                     ADGTVSQNWIVNIVKSTVVSNKAEIVGLYSSVLKDVQINSVTNTVTATVPYFIQDCYQ
                     MDIQVSAGAMVMNPLQTQCFDGSNPMIRTVQVKAEDGTTIKTWTINFVREAKPVGADC
                     DKLATAVKGYNTIPFAQYQDTYFMEYTMENNGTFEVDACTSNMMLGAYIILDCETIDN
                     SALLTYCKDPNFASKSQKEYTKGQKVKIAVFRVSENSYPTVSGVMI"
BASE COUNT          547 a          284 c          258 g          575 t
ORIGIN      
        1 aatcataacc ccgctcactg taggataact attttctgaa actctaaata ctgcaatttt
       61 tactttttga ccttttgtat attctttttg tgatttagaa gcaaaattag ggtctttaca
      121 atatgtcaat aaagccgaat tatcaatagt ttcacaatcc aatataatat atgcacctag
      181 catcatatta cttgtacacg catcaacttc aaatgtaccg ttattttcca tagtatactc
      241 cataaagtaa gtatcttgat actgtgcgaa cggaattgta ttatatcctt taactgcagt
      301 agcaagttta tcgcaatcag cacctacagg ttttgcttca cgcacaaaat taattgtcca
      361 cgttttgatt gtagtaccat cttctgcttt tacctgaact gtacgaatca ttggatttga
      421 accatcaaaa cattgtgttt gcaaaggatt cataaccata gcaccggctg atacttgtat
      481 atccatctga taacaatctt gaataaaata tggaactgta gctgttactg tatttgttac
      541 agaattaatt tgaacatctt ttaaaaccga agaatacaat ccaacaattt cagctttatt
      601 gctaactaca gtagatttca caatatttac tatccaattt tgagaaacag ttccatcagc
      661 acttgttaca gtaatagtag tagttccatt attgaaaaac aactgactac ctggacacag
      721 tgtaatacca ctacttgttt taatagtagc accaggtgat actttaaaat ctatagttgt
      781 ttcagaaatt gatgcagatt gagctactgt aagattaata gttctggtaa tactatttat
      841 cacagcattt gaaacttgtt gcattgacga aaaactagta attgcaacag attttgtaga
      901 atattcattc aaagtccaat ttacggtagt actaagattt ctacttctga attgaatata
      961 taacgtagta tctttttcaa taggcatagt tataccttgc aaattattac cacatggcat
     1021 aaatacgtta ttataatagc ctccgccatg actatcaaac ggtgaaggac atgagaaata
     1081 aatagaaggc catatttgta gttgttggaa ttgttcatta ttaaaatcta aatgaacaat
     1141 tgtatttgct ttggcattat atgaatacca ataattacca tacacagttg tagtttgttg
     1201 tgctcctaac gataaaggtt tagcatcggc acacacaaca tttgcaggtt gtgattgatt
     1261 tgataggtat tttgattgaa ttgtatacgg catagttggt gcttgctctg aaaatacaat
     1321 cagcatctta tattttttat ttgcttgtgg ccaaaataca attgcagaat ccatcattag
     1381 atacggcact gtagatttca gattttcact caatggtgta cacacatcaa ataaaaatac
     1441 atttgcctca atattaagat ttgaacttaa agaaaccgtt ccggattgtg gatatgataa
     1501 ctcaagccaa gtgatatttc cggtaggttg aactactgtc tgttcttgtg aaattacaat
     1561 aggattttga catgtatacc ccggaggtaa tacagcatct tttataaagt ctaaattcca
     1621 attttgcgca tgagtaaacc attgaataat ataggttttc cctt
//
LOCUS       DAOTOW010000567         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57103_length_1663_cov_3.213308, whole genome shotgun sequence.
ACCESSION   DAOTOW010000567 DAOTOW010000000
VERSION     DAOTOW010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57103_length_1663_cov_3.213308"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="P5243_09280"
     CDS             complement(<1..1386)
                     /locus_tag="P5243_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRS19684.1"
                     /translation="MNDLFIIAPFTIGLIILLGILIGKYVVWTIQLSQIDKLRILHGF
                     FTVRTFRAIREIFWESLLHRNIWKTNPLLGYMHMSFALGWLLLIIVGHIEAYVQASSL
                     SVPPWIPIFFRYFEHEHFYGARFFAHTMDVLLLYILSGVVLAYGKRINSKLFGIKRTT
                     KLKPKDRISLIALWSIFPLRLFAESATAVLHGNGGFLTSGIVFRYLQVTPNIEYTLWV
                     LYSCSLAIFFITLPFTRYMHIPTEVFYIFLKEYGIKLKKQFNSYSKIQVYSCSRCGIC
                     IDACQMHHAQISNNQSVYILKNIRDENLTDRKLYNCLLCGRCEDACPVNINLNDLRIS
                     QRIKSSKEYNSSYDFLEYSITEKADVVYFAGCMTHLTPGIKKSILTVLDTAGVNYLFL
                     DKDKGACCGRPLMQAGQIEAAKKLIAYNRQQILESGAHTLLVSCPICYKVFRDDYELG
                     QINIRHHSEYFL"
     gene            complement(1389..>1663)
                     /locus_tag="P5243_09285"
     CDS             complement(1389..>1663)
                     /locus_tag="P5243_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRS19685.1"
                     /translation="ISYIIKNEPSFSSCISCGSCTATCSAANLTFFNIRRLNIQIRLG
                     EFENIRQEIDKCMLCGKCLLVCPRGINTRNVIMLIKKSLVTFNLVE"
BASE COUNT          599 a          297 c          228 g          539 t
ORIGIN      
        1 taaaaagtat tctgaatgat ggcgaatatt tatttgtccc aactcataat catcacgaaa
       61 aactttataa catataggac acgaaacgag caaagtatgg gcacctgatt ctaatatttg
      121 ctgcctatta taggctataa gttttttagc tgcttctatt tgccctgctt gcataagtgg
      181 tcggccacaa caagctcctt tatctttatc taaaaataaa taattaacac cggcagtatc
      241 taacaccgta agtattgatt tttttatacc gggtgttaaa tgagtcatac aacctgcaaa
      301 ataaactaca tcagcttttt ctgtaattga atattctaaa aaatcgtaac tcgaattgta
      361 ttctttactt gattttattc gttgagaaat tcgtagatca tttaaattaa tatttaccgg
      421 acacgcatct tcgcatcgtc cacagagtaa acaattatac agttttctat cagtaagatt
      481 ttcatcacga atatttttta aaatatacac cgactgattg tttgatattt gagcgtggtg
      541 catttgacac gcatctatac aaattccaca acgcgaacaa gaatacactt gaatttttga
      601 atatgaatta aactgctttt taagttttat tccatattct ttcaaaaata tataaaacac
      661 ttcggtagga atatgcatat atcgagtaaa aggcagagta ataaaaaata tagccaaact
      721 acaagaatac aatacccata gtgtatattc aatgtttgga gtaacttgca aataacgaaa
      781 tactataccg cttgtcaaaa aacctccatt accatgtaat acagcagtag cactttcggc
      841 aaacaatcgt aatgggaata tactccacaa tgcaataaga gaaattctat ctttgggttt
      901 gagtttagtt gtacgtttta taccaaacaa tttactgttt attcgtttac catatgctaa
      961 tactactcca cttaaaatat ataagagtag cacatccata gtatgagcaa aaaatcgagc
     1021 gccataaaag tgttcgtgtt caaaatatct aaaaaatatt ggaatccacg gcggaaccga
     1081 caaagatgaa gcttgcacat acgcttctat atggcctact attattaaca acaaccaacc
     1141 caaagcaaaa ctcatgtgca tataccccaa taaaggattt gttttccaaa tatttctatg
     1201 taataaactt tcccaaaaaa tttcacgaat cgctctaaac gtacgtacag taaaaaaacc
     1261 atgcaatatt cgtaatttat caatttgcga caattgaata gtccatacta catattttcc
     1321 tatgagtata cctagaagta taattaaacc tatagtaaag ggagctatta taaataaatc
     1381 gttcattatt attcaaccaa attaaatgtt accaaacttt tttttatgag cataattaca
     1441 tttcgggtat ttattcctcg cggacatact aaaagacatt ttccgcatag catacattta
     1501 tctatttctt gacgaatatt ttcaaactct cctagtcgta tttgaatatt taatctacga
     1561 atattaaaaa aagtaaggtt tgcggcactg cacgtagcag tacatgaacc acacgatata
     1621 caagaactaa atgatggttc gtttttaata atatatgata tta
//
LOCUS       DAOTOW010000568         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57415_length_1657_cov_2.365793, whole genome shotgun sequence.
ACCESSION   DAOTOW010000568 DAOTOW010000000
VERSION     DAOTOW010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57415_length_1657_cov_2.365793"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /gene="galT"
                     /locus_tag="P5243_09290"
     CDS             complement(<1..430)
                     /gene="galT"
                     /locus_tag="P5243_09290"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRS19686.1"
                     /translation="MNEFTLYPHRRYNSLTGEWILVSPHRTQRPWQGHKETKQTITMP
                     SYDNDCYLCPGNKRAGNQINPHYSNTFVFTNDFSALLPTTPKIENTEIFFTTHTEQGI
                     CKVICFSPHHNLTIPEMDITSIEHVIRTWIQEYGELSKIDA"
     gene            complement(434..1597)
                     /locus_tag="P5243_09295"
     CDS             complement(434..1597)
                     /locus_tag="P5243_09295"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRS19687.1"
                     /translation="MTIQELQTLIISKYGHSHEPLHIYFSPGRINLIGEHTDYNGGYV
                     LPAAISYGTYCCIRKNSIPYIECSSVQIPQHYTILLNDISKKNNLGWANYIIGVYAQF
                     VKKQLQSHNGYQILIYGNVPQGAGLSSSASLEMSVAYAINDIEQYNIPVIELASMCQQ
                     AEHEYAGVHCGIMDQFASGMGKKDSAIYLNCKTLDFSYVPIVLDEYNIIITNSNSPHA
                     LESSKYNERVNECKQAVKYIQQYYTISQLSEISVNEFKTIEKKIPELYRKRARHVITE
                     IDRTQKAVEALNSGKISLFGNLMNESHYSLHHNYEVTGMYLDTLTSVAQSIDGCIGSR
                     MTGGGFGGSTISIVHNSSIQNFITIISTEYHKITGIEANCYTAQISNGTHKIV"
BASE COUNT          524 a          249 c          255 g          629 t
ORIGIN      
        1 ttgcatctat ttttgataat tctccatatt cttgtatcca agttcttatt acatgttcta
       61 tagaagttat gtccatttca ggaattgtaa gattgtggtg tggcgaaaaa catattactt
      121 tacaaattcc ttgttctgta tgagttgtaa aaaaaatctc agtattttca atcttaggtg
      181 ttgttggtag taatgcagaa aaatcgtttg taaaaacaaa tgtgttgcta taatgaggat
      241 ttatttgatt accagccctt ttatttccgg gacacaaata acaatcatta tcgtaagaag
      301 gcattgtaat agtttgttta gtttctttat gtccttgcca cggtcgttgt gttctatgag
      361 gtgatacaag tatccattct cctgttaaac tattgtatct gcgatgtgga tatagtgtaa
      421 attcattcat gtattataca attttatgtg tcccattact aatttgtgct gtataacaat
      481 ttgcttctat tccggtaatt ttatgatatt cagtagaaat aatggtaata aaattttgaa
      541 ttgaagaatt atgaacaata gaaatagtag aacctccaaa tcctcctccg gtcatgcgtg
      601 aacctataca accatcaatt gattgagcaa ctgaagttag tgtgtctaaa tacattcctg
      661 ttacttcata attgtgatgt aatgaataat gtgattcatt cattaaatta ccaaataatg
      721 atatttttcc tgaatttaaa gcttctacag ctttttgagt tctatcaatt tctgtaataa
      781 catgacgagc tcgttttctg taaagttcag gaattttttt ttcaattgtt ttaaattcat
      841 ttactgaaat ttcagataat tgtgaaattg tatagtattg ttgaatatat ttcaccgctt
      901 gtttacattc attcacacgt tcattatatt tacttgattc taatgcgtga gggctgttag
      961 aatttgtaat tatgatatta tactcatcta aaacaattgg tacatatgaa aaatctaatg
     1021 ttttgcaatt caggtatata gcggagtctt tttttcccat gcctgatgca aattgatcca
     1081 taattccgca atgtacacct gcatattcat gttctgcttg ttgacacatg cttgctaact
     1141 ctataacagg tatattgtat tgttctatgt catttatagc atatgccaca ctcatttcta
     1201 gcgaagccga agatgataaa ccggcgcctt gaggaacatt accataaatc agtatttgat
     1261 atccattgtg tgattgtaat tgttttttta caaattgagc ataaactcca ataatataat
     1321 tagcccaccc caaattattc tttttgctaa tatcatttaa aagaattgta taatgttgtg
     1381 ggatttgtac agacgaacat tcaatatagg gaatagagtt ttttcgtata caacaataag
     1441 taccatatga tattgctgcc ggtaatacat atcctccatt ataatcggta tgctcgccta
     1501 ttaaattaat tctaccgggt gaaaagtata tatgcaatgg ttcatgagaa tgtccatatt
     1561 tagaaataat taatgtttgt aattcttgta ttgtcatgta aaaacagttt tttttccaaa
     1621 ttcaaacaaa tatagtgtaa aaaaaacaat ataacac
//
LOCUS       DAOTOW010000569         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57431_length_1657_cov_1.963795, whole genome shotgun sequence.
ACCESSION   DAOTOW010000569 DAOTOW010000000
VERSION     DAOTOW010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57431_length_1657_cov_1.963795"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="P5243_09300"
     CDS             complement(<1..224)
                     /locus_tag="P5243_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HRS19688.1"
                     /translation="MKYLIVISGPTAVGKTKVAIDIALHYKTEIISADSRQFYKEIPI
                     GTAAPTFAELQTVGHHCIGNLHITDYYNAF"
     gene            complement(240..803)
                     /locus_tag="P5243_09305"
     CDS             complement(240..803)
                     /locus_tag="P5243_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19689.1"
                     /translation="MIYKFRVISDENETFLRIIEISKNDTFLKLHDAIIAACDYDPAQ
                     MTSFFVSNKDWEKLQEITLFDMKDESTKTIPMHKAVLKDYVSKVDDTLIYIFDFFADR
                     ALFLTLVEIKKEDPKMHYPVCSYETGNPPIQMLQDENEFTQMFNDLEDGEDFEDFDDL
                     DDDIKGEFDEYNEFDDFGGVNDYDSEY"
     gene            complement(807..>1657)
                     /locus_tag="P5243_09310"
     CDS             complement(807..>1657)
                     /locus_tag="P5243_09310"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRS19690.1"
                     /translation="PHNLYAWIEKYTFQKNNTKTVALILAGNIPVVGFHDVLCVLMSG
                     HRLRVKLSSKDTVLMTYILQTLQAIEPEFTNKIYLEPHIVKDFDAIIATGSTNSSRYF
                     EYYFTKYPHIIRKSRNSIAVITESDTIEQYTAIGNDIFNYFGLGCRNVSKLYVPHNFK
                     VEMFFEAIEQFNYVATHTKYMNNYEYNKSIYLVGNQSHYDNGFVLLKEDNAISSPLAV
                     VYFEKYMNFAHVQKYVETIQDTIQCIVSTRNDFYKKNVLPGCSQRPAVWEYADDIDTM
                     EFLLQI"
BASE COUNT          562 a          281 c          209 g          605 t
ORIGIN      
        1 ttaaatgcat tataataatc ggtaatgtgt aaattcccaa tacaatgatg accaactgtt
       61 tgtaattcag caaacgtagg agcagctgtt ccaataggta tttctttata aaactgcctt
      121 gaatcggcag atatgatttc ggtcttataa tgtaatgcta tatctatagc tacctttgtt
      181 ttaccaactg cagtaggtcc tgaaattaca ataagatact tcattcacaa gcaatcatat
      241 taatattctg aatcataatc atttactcca ccaaaatcat caaactcatt atactcatca
      301 aactcacctt ttatatcatc atcaaggtca tcaaaatctt caaaatcttc accgtcttct
      361 aaatcattaa acatttgggt aaattcattt tcatcttgaa gcatttgtat aggaggattt
      421 ccggtttcat aagaacacac aggataatgc atctttggat cttctttttt aatttctacc
      481 aaagtcaaaa acaatgctct gtcagcaaaa aaatcaaaaa tataaattaa tgtatcatct
      541 acttttgaca cataatcttt gagaacggct ttgtgcattg gaattgtttt agtgctctcg
      601 tctttcatgt caaacaatgt aatttcttgc aatttttccc aatccttatt tgacacaaaa
      661 aatgaagtca tttgtgcggg atcataatca catgcggcaa ttatagcatc atgcaacttt
      721 aaaaatgtgt cattttttga aatctcaatt atgcgtaaaa atgtttcatt ttcatcgcta
      781 atcactctaa atttgtaaat catagtttaa atttgtaata aaaattccat agtatcaata
      841 tcatctgcat attcccatac tgcgggacgc tgagaacaac cgggcaatac atttttttta
      901 taaaaatcat tgcgagtact cactatacat tgaatagtat cttgtatagt ttctacatat
      961 ttttgtacgt gtgcaaaatt catatatttt tcaaaatata caacagctaa tggcgaactt
     1021 attgcgttat cttcttttaa taatacaaat ccattatcat agtggctctg atttccaact
     1081 aaataaatag atttattgta ctcataatta ttcatatatt ttgtatgagt tgccacataa
     1141 ttaaattgtt ctatagcttc aaaaaacatt tctaccttaa aattgtgggg gacatacaat
     1201 tttgaaacat ttctacaacc taatccaaaa taattaaaga tatcatttcc tatagcggta
     1261 tattgttcta tagtatcaga ttctgtaatt acagcaattg aatttctact tttacgaatt
     1321 atatgaggat attttgtaaa ataatattca aaataccgcg atgaattagt acttcctgta
     1381 gcaataattg catcaaaatc tttaacaata tgaggttcta aatatatttt attggtaaat
     1441 tcaggttcta ttgcttgtag cgtttgtaaa atataagtca ttaacactgt atcttttgat
     1501 gataatttta cacgtaatct atgaccactc atcaatacac acaatacatc atgaaatccc
     1561 accactggaa tatttcctgc aagtataaga gctactgttt ttgtattatt cttttggaat
     1621 gtatattttt caatccatgc atacaggtta tgaggtt
//
LOCUS       DAOTOW010000570         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57595_length_1653_cov_5.185857, whole genome shotgun sequence.
ACCESSION   DAOTOW010000570 DAOTOW010000000
VERSION     DAOTOW010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57595_length_1653_cov_5.185857"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="P5243_09315"
     CDS             <1..201
                     /locus_tag="P5243_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRS19691.1"
                     /translation="LKHGADIHANKDTALRWATENGRYKVVKLLLNNGANIHANNDDA
                     LRWAKYNGDEKMIKLLESYINK"
     gene            213..881
                     /locus_tag="P5243_09320"
     CDS             213..881
                     /locus_tag="P5243_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRS19692.1"
                     /translation="MKYYKIVNPEGHHGLIYREGLNIDPLPFNPSGDCEPGGIYFAKE
                     DILAFMDYGTELYEVESIGDVYENPGKPKKYKAHEVNLKYIGKVIDNIEMLIKEGADI
                     HADNDTVLYWAAKNGHYNVVKLLLENGTDIHANRDYALRLAANNGYYDVVKLLLENGA
                     NVHVNNDIALILAVNKRHYNVVKLLIEYGADIHANDYVELRLAAYNNDEKMIKLLESY
                     ITKK"
     gene            887..>1653
                     /locus_tag="P5243_09325"
     CDS             887..>1653
                     /locus_tag="P5243_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRS19693.1"
                     /translation="MKYYKIVDPEGHNGLIYHEELNVDPLPFNPGGDCEPGGIYFAKE
                     DILAFVNYGTELYEIEPIGDVYENPGMPKKYKAHEVNLKYVGKVIDNIEMLIKEGADV
                     HADYDCVLQWAAENGHYNVVRMLLENGADIHADRNYTLRWAVYNGYHKVVELLLENGT
                     DIHAENDYALRWAAYNGRHKVVELLLKHGADIHAENDCSLRWASENGHYNIVKLLLKY
                     GANVHANDDYALRWASRNGHYEIVRLLIEHSANIHAN"
BASE COUNT          594 a          194 c          366 g          499 t
ORIGIN      
        1 ctcaaacatg gtgcagatat tcatgctaat aaagatactg cattacgttg ggcaacggag
       61 aatgggcgtt ataaagttgt gaaactgctg cttaataatg gtgcaaatat tcatgcgaat
      121 aatgatgacg cattgcggtg ggcaaaatat aatggtgatg aaaagatgat taagttattg
      181 gaaagttaca taaacaaata aaacaaaata atttgaaata ctacaagata gtaaacccag
      241 agggtcatca tggcttaata tatcgtgagg gtctaaatat tgaccctcta ccattcaatc
      301 caagtggtga ttgcgaaccg ggtggaatat acttcgcaaa agaagacata ttggcgttca
      361 tggattacgg aacagaattg tatgaagttg aatctattgg agatgtatat gaaaatccgg
      421 gtaaacctaa aaaatacaag gctcatgaag ttaatttgaa atatatagga aaagtaattg
      481 ataatattga aatgttgatt aaggaaggtg ctgatataca tgcagataat gatactgtac
      541 tatattgggc agcaaaaaat gggcattata atgttgtgaa attattgctc gaaaacggta
      601 ctgatattca tgcaaataga gattatgcgt tacgcttggc agcaaataat ggatattatg
      661 atgttgtgaa attattgctc gaaaacggtg ctaatgttca tgtgaataat gatattgcat
      721 tgattttagc agtgaataaa agacattata atgttgtgaa attattaatt gaatatggtg
      781 cagatattca tgcaaatgat tacgttgaat tacgtttggc agcatataac aatgatgaaa
      841 agatgattaa actattagaa agttacataa ctaaaaaata acaattatga aatactacaa
      901 aatagttgat ccagaaggtc ataacggttt aatataccat gaggaattaa acgttgaccc
      961 gctaccattc aatccgggcg gtgattgtga accgggtggg atatatttcg ctaaggaaga
     1021 tattcttgca tttgtaaatt atggcactga attatacgag attgagccta tcggtgatgt
     1081 atatgagaat ccgggtatgc caaagaaata caaagctcat gaggtcaatt tgaaatatgt
     1141 tgggaaggtg attgataata tagagatgtt gattaaggaa ggtgctgatg tacatgcaga
     1201 ttatgattgt gtattacaat gggcagcgga aaatggtcat tataatgttg tgagaatgtt
     1261 gctcgagaat ggtgctgata ttcatgcaga tagaaattat acgttacgtt gggcagtata
     1321 taatgggtat cataaagttg ttgaattgtt gctcgagaat ggtactgata ttcatgcaga
     1381 aaatgattat gcattacgtt gggcagcata caatggacgt cataaagttg ttgaattgtt
     1441 acttaaacac ggtgctgaca ttcatgcaga aaatgattgt tcattacgtt gggcatcaga
     1501 aaatggacat tataatattg taaaattatt attaaaatac ggtgcgaatg ttcatgcgaa
     1561 tgatgattat gcattacgtt gggcatcaag aaatggacat tatgaaattg tgagattatt
     1621 aattgaacat agtgcgaata ttcatgcaaa taa
//
LOCUS       DAOTOW010000571         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57714_length_1651_cov_4.006266, whole genome shotgun sequence.
ACCESSION   DAOTOW010000571 DAOTOW010000000
VERSION     DAOTOW010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57714_length_1651_cov_4.006266"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="P5243_09330"
     CDS             <1..866
                     /locus_tag="P5243_09330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS19694.1"
                     /translation="GWEAMFSPKPSIPSNKYIQTCSKNGKLLAHKLQSYATRFGKPIH
                     KAHILEIGCNTGAASYHLAQLGALKVTGTEFNGYKVESIQNTQTTQQALEEVDANLHY
                     MRSVLSQSFSQKDNVTFINDDICNSQLPHNSYDIICSFDVLEHLHNTAQAFKSMALLL
                     KPGGLIIHDYNPFFALNGGHSLCTLDFEWGHARLNNTDFETYISMYRPNEYEQALAFY
                     YKGLNRVTIHEMQSHIIDAGLTIEACIPYTRPQHIHTITHSILSQVQTHYPKATALDL
                     ISPRVVIIARK"
     gene            956..1585
                     /locus_tag="P5243_09335"
     CDS             956..1585
                     /locus_tag="P5243_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRS19695.1"
                     /translation="MMDLDAFEKLPLLGILRGIESKHVEPLAITVGKTGLPAIEITMN
                     TPNAAALIKEMKYYSRGKFSVGAGTVLTFRDLDTAVAAGASFIVMPSLQLEVIRICVS
                     QKIPVFPGAFTPTEIYKAWEMGATMVKVFPSSIVGPAYFSEVKGPFDSIKLLACGGIN
                     QQTIGEFFKKGANAAAFGASIFKKEWLETEHYDAIGNEITGLIEAYKKI"
BASE COUNT          582 a          292 c          279 g          498 t
ORIGIN      
        1 ccgggtggga agcaatgttt tctccaaaac cttcaattcc atcaaacaaa tacatacaaa
       61 catgttctaa aaatggtaaa ttattagcac acaaattaca aagctatgct acacgatttg
      121 gtaaacctat acacaaagct catatacttg aaataggctg taatacaggt gctgcatcat
      181 atcatcttgc acaactggga gccctaaaag ttacaggcac cgaatttaac ggatacaaag
      241 tagagtcaat acaaaacact caaaccacac aacaagcttt agaagaggtt gatgcaaact
      301 tacactatat gcgttcagtt ttatctcaat cgttttcaca aaaagacaat gttaccttta
      361 taaatgatga tatttgcaat tcgcagttac ctcataattc atacgatatt atttgtagtt
      421 ttgatgtttt ggaacattta cacaacactg cacaagcatt caaatctatg gctctattac
      481 taaaaccggg aggtttaatt atacatgatt acaatccatt ttttgcactc aatggtggac
      541 atagtttatg tactcttgat tttgaatggg gacatgcccg attaaacaat accgattttg
      601 aaacatacat ttctatgtat cgcccaaatg aatacgagca agccttagca ttttattaca
      661 aaggattgaa ccgtgtaact atacatgaaa tgcaatcaca tattattgat gcaggtctta
      721 ctatagaagc atgtataccc tataccagac ctcaacacat tcatacaata acacatagta
      781 tactttcaca agttcaaact cattatccaa aagcaacagc tctcgattta atatcgccac
      841 gggtagttat tatagctcgc aaataatttt ttattggatt ttgattaaaa attatgtatt
      901 tttgatatgt tgctatatac ttggagcaca ataacttttt aaacatataa cacctatgat
      961 ggatttagat gcatttgaaa aattaccttt attaggaatt cttagaggta tagaatcaaa
     1021 acacgtagag ccacttgcaa taacagttgg taaaacaggg ttacctgcaa tagaaattac
     1081 catgaatact ccaaatgctg ctgcattaat taaagaaatg aaatactatt cacgcggtaa
     1141 attttctgtt ggagcaggta ctgttttaac attcagagac cttgatacag ctgtggctgc
     1201 aggagcatca tttattgtaa tgccatcact tcaattagaa gtaattagaa tatgtgtttc
     1261 acaaaaaata ccggttttcc cgggagcatt tactcctaca gaaatatata aagcatggga
     1321 aatgggggca acaatggtta aagtatttcc atcatcaata gttggtcctg catatttttc
     1381 tgaagttaaa ggcccatttg attctattaa actgcttgcc tgtgggggta taaaccaaca
     1441 aacaataggt gaattcttta aaaaaggggc taatgccgct gcatttggag caagtatttt
     1501 caaaaaagaa tggttagaaa ccgaacacta tgatgcaata ggaaatgaaa taacaggatt
     1561 gattgaagca tataaaaaaa tataactata tactatcggc ggcggtatgg ccagtaaggg
     1621 attgcgggtg atttcctgtc aagttacacg a
//
LOCUS       DAOTOW010000572         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57726_length_1651_cov_3.390351, whole genome shotgun sequence.
ACCESSION   DAOTOW010000572 DAOTOW010000000
VERSION     DAOTOW010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57726_length_1651_cov_3.390351"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="P5243_09340"
     CDS             complement(<1..1101)
                     /locus_tag="P5243_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19696.1"
                     /translation="METQSSHIWKFSRVGGVNRVNIESAQDLLHLDELDQKLWTVLSC
                     PVQGLNIDEATLKLIDTDADGRIRVPEVLEAVKWTCSMLINPEIILQQPESIQLHHIQ
                     TNFDDGKQMLASAKQILSNLEKSSQDYISISDTADSTAIFAKTGFNGDGIITSISTDL
                     EQLQKTIEFCISTIGFVTDRSGLAGVNEEIVNEFYTNCQEYLSWYSLAEQNKAILPFG
                     DNTENCLQLFEVVQSKIDDYFLRCQIAEFDANAIDVLHNIVSQIESISSKDITKCIDE
                     IAALPLAKIEPNKPLSLISGINPAWEEKMHQFYTDIVIKMFPKNTHITHSEWKNIVDI
                     FNEYIQWKSNKKGERVEALGIETIRNILRENNK"
     gene            complement(1222..>1651)
                     /gene="rplI"
                     /locus_tag="P5243_09345"
     CDS             complement(1222..>1651)
                     /gene="rplI"
                     /locus_tag="P5243_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRS19697.1"
                     /translation="KDMPNLGLKNDVVTVKPGYGRNYLIPQGIGILATESAKKQHAEI
                     MKQRAHKEEKLKKDAAELAAKLETVSLTVGTKASSTGKIFGSVNTIQIAEALQKAGYD
                     IDRKKIVLKEHAIKDLGSYSCKIVLYKGIEVKIPFEVVAE"
BASE COUNT          526 a          280 c          209 g          636 t
ORIGIN      
        1 tttattattt tcacgtaata tgtttcgaat tgtttctatt cctaacgctt caactcgttc
       61 tcctttttta ttagatttcc actgaatata ttcattaaaa atatcaacaa tatttttcca
      121 ttcactatgt gttatgtgag tgtttttagg aaacatttta attacaatat cagtatagaa
      181 ctgatgcatt ttttcttccc atgcaggatt aatacccgaa ataagagata aaggtttatt
      241 cggctctatt ttggctagtg gcaatgctgc tatttcatct atacatttcg ttatatcttt
      301 tgaactaata gattctattt gcgatacaat attatgcaac acatctattg catttgcatc
      361 aaattcggca atttgacaac gtaaaaaata atcatcaatt tttgattgta caacctcaaa
      421 taattgtaaa caattttcag tattatcacc aaaaggaaga attgctttat tttgttcagc
      481 taacgaatac catgataagt actcttggca attagtataa aattcattta caatttcttc
      541 atttactccg gctaaaccac ttctatcagt aacaaaacca attgtagaaa tacaaaattc
      601 aatagtcttt tgtaattgtt ctaaatcagt agaaatagat gtgataatac catcaccatt
      661 aaatcctgtt tttgcaaata ttgcagtact atctgcagta tcacttattg aaatataatc
      721 ttgactagat ttttctagat ttgaaagaat ttgcttagct gatgcaagca tttgtttccc
      781 atcgtcaaaa tttgtttgta tgtgatgtaa ttgaatactt tcgggttgtt gtaaaataat
      841 ttcaggatta ataagcatgg aacatgtcca ttttacagct tccaatactt caggcacccg
      901 aattcgtcca tcagcatcag tatcaataag ttttaaagta gcttcatcta tgttaagtcc
      961 ttgcaccgga caacttaaaa ctgtccataa tttttgatct aattcatcta aatgaagtaa
     1021 atcctgtgca gattcaatat ttaccctatt tactcctcca actctagaaa acttccaaat
     1081 atgtgaagat tgtgtttcca tattatttaa atttataatt ataaggcaat atataaaaaa
     1141 aagcaattgt agaattgctt tgcataaaaa aaaggctgtc aattatttga cagccttttt
     1201 ttttatattt tttagaaaaa attattcagc aactacttca aaaggaattt taacttcaat
     1261 acctttatac aatacaattt tacatgaata acttcctaaa tctttaattg catgttcttt
     1321 taacactatt tttttgcgat caatgtcata tcctgctttt tgtaaagctt cagcaatttg
     1381 aatagtatta acagatccaa agattttacc tgtagagctt gcttttgttc caacagttaa
     1441 actaactgtt tccaatttag cagctaattc ggctgcatct ttttttagtt tttcttcttt
     1501 atgagcacgt tgcttcataa tttcggcatg ttgctttttt gccgattctg ttgcaaggat
     1561 acctatacct tgaggaatca aataatttct tccatatccg ggttttaccg ttacaacatc
     1621 attttttaag cctaaattag gcatatcttt t
//
LOCUS       DAOTOW010000573         1650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57776_length_1650_cov_3.529154, whole genome shotgun sequence.
ACCESSION   DAOTOW010000573 DAOTOW010000000
VERSION     DAOTOW010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57776_length_1650_cov_3.529154"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..639)
                     /locus_tag="P5243_09350"
     CDS             complement(13..639)
                     /locus_tag="P5243_09350"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRS19698.1"
                     /translation="MEYNTTQKKLILPEYGRNVQMMAEHLLTIENRQRRTEAAHELVA
                     IMTNMNPLVKETKDYKQKIWDFLAELCEHKLDIDFPYPITKASELPPPDKLEYNTSKI
                     KFRHYGLIIERLIEAASKFEDGPDKDVLIKMIANHMKKTYIAWNQKSVNDSIIINDLY
                     KLSHGKLNLSEETKLMHVSVAKEQNNNHNNKKHNNKQKNNKQKNNKRK"
     gene            complement(696..>1650)
                     /locus_tag="P5243_09355"
     CDS             complement(696..>1650)
                     /locus_tag="P5243_09355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRS19699.1"
                     /translation="NSLSLWDIISKIELATDILNKATISKIISFRTLIQNFIQDLQIA
                     DAYSLGSRIIEQSGIFAELKADTSIEGKSRFDNIQELLNGIKEIVEQETEEIYLHNYI
                     ERIALLTDIDSDETEKLNKITLMTVHSAKGLEFEHCFIVGLEDGLFPSTMNKNNPHEI
                     EEERRLFYVALTRAKQTATLSYASSRRKWGNLEICKPSQFINDIDSKYIEDKRIQGFS
                     SNNYSFLNESDYQKKQIKPQTFNSFTKPTQSTKPIKIEHFEADAPEKIQSGQTIIHAT
                     FGKGIVKEIIGKYPDSVAHIHFSASGEKKLLLKFAKLKVVE"
BASE COUNT          467 a          227 c          257 g          699 t
ORIGIN      
        1 aaaaacagcc atttatttgc gtttgttatt tttttgcttg ttattttttt gtttattatt
       61 gtgttttttg ttgttgtgat tattgttttg ttctttggca acacttacat gcataagttt
      121 agtttcttca gataaattga gtttgccatg tgaaagttta tataaatcat ttattataat
      181 tgaatcattt accgattttt gattccatgc tatatatgtt tttttcatat ggttagcaat
      241 catttttatc agtacatctt tatcggggcc atcttcaaat tttgaagctg cttctattaa
      301 tctttcaatt attagtccgt aatggcggaa ttttatttta ctggtgttgt attctaattt
      361 atcgggagga ggtaattcag aagcctttgt tattggatag gggaagtcta tatctaattt
      421 atgctcgcat aattctgcca aaaaatccca aattttttgt ttataatctt tagtttcttt
      481 tactaaagga ttcatattag tcataatagc tacaagttcg tgagctgctt cggttctacg
      541 ttggcgattt tctattgtaa gaaggtgttc tgccatcatt tgcacatttc taccgtattc
      601 gggtaaaatt agtttttttt gtgtagtatt atactccata tatatttgat attatgatac
      661 aaaaatacga aaaatattga aagtttaatt ggtaattatt ctacaacttt gagcttagcg
      721 aattttaata ataatttttt ttcgcccgaa gcagaaaaat gtatatgtgc taccgagtca
      781 gggtatttac caatgatttc tttgacaata cctttcccaa atgttgcatg tataattgtt
      841 tgccccgatt gtattttttc gggagcatct gcttcaaaat gttctatttt tataggtttt
      901 gttgactgtg taggtttagt aaatgaatta aatgtttgag gttttatttg ttttttttga
      961 taatcagatt cattcaaaaa gctataatta ttgcttgaaa atccttggat tcttttgtct
     1021 tctatgtatt tagagtcgat atcgttaatg aattgacttg gtttacatat ttctaaatta
     1081 ccccattttc tacgcgaact ggcataagat aatgttgctg tttgttttgc acgagtaaga
     1141 gcaacataaa ataatcttcg ttcttcttca atttcgtgag gattgttttt attcattgta
     1201 gaagggaata acccgtcttc aagtcctact ataaagcaat gttcaaattc taatcccttg
     1261 gcagaatgaa cagtcataag ggtaatttta tttaattttt ctgtttcatc tgaatctata
     1321 tctgtaagca atgcaatacg ttctatgtaa ttatgtagat atatttcctc agtttcttgt
     1381 tctacaattt cttttatacc attgagtaat tcttgtatat tatcaaatct acttttgcct
     1441 tctatgctag tatcagcttt taattccgca aaaatgcctg attgttcaat aattcgtgaa
     1501 cctaatgaat atgcatcggc aatttgtaaa tcttgtatga aattttggat aagtgttcta
     1561 aagctaataa ttttagaaat agtagcttta tttaaaatat ctgttgcaag ttcaattttt
     1621 gaaattatat cccacaatga taaagaatta
//
LOCUS       DAOTOW010000574         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_57973_length_1646_cov_3.010685, whole genome shotgun sequence.
ACCESSION   DAOTOW010000574 DAOTOW010000000
VERSION     DAOTOW010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57973_length_1646_cov_3.010685"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="P5243_09360"
     CDS             <1..476
                     /locus_tag="P5243_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRS19700.1"
                     /translation="SKKVIIALFTSEETRLDGSNALAKRIKAQQIPLKYVINFDMIGT
                     TMIDAPKKVFITGFDKSNLAVTANTLLQEDFLLKDAQTEDMELFRYSDNFSFYRELNI
                     PAHTLSTFNMRNFDCYHRTCDEVSEIDVKNLESIIQKSTTFIIELLKQNSQIALN"
     gene            503..>1646
                     /gene="clpX"
                     /locus_tag="P5243_09365"
     CDS             503..>1646
                     /gene="clpX"
                     /locus_tag="P5243_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRS19701.1"
                     /translation="MKQECSFCGRKKTEVSMLLTGLSGNICSDCVGQAIEIINQELPA
                     AKRKSTKKSKILKPIEIKQHLDEYVIGQESAKKILSVAVYNHYKRISQELTDDVEIEK
                     SNIIMVGETGTGKTLLAKTIAKMLDVPFAIVDATVLTEAGYVGEDIESLLVRLLQDAD
                     YDVPRAEQGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVNVPPQGGR
                     KHPDQKMIAVNTKNILFICGGAFEGIQKVIARRLNTKVVGFGAKEKTQQIDKNNFLQY
                     IAPQDLRTFGLIPEIIGRLPVLTYLNPLDRDALRRILTEPKNSLIKQYKALFHLDNIE
                     LSFEEETYDYIVDKAIEFKLGARGLRSICEAIMVDAMFEYPSKNIKE"
BASE COUNT          615 a          259 c          314 g          458 t
ORIGIN      
        1 tatcaaaaaa agtaataatt gctctattta cttcagaaga aaccagattg gacggttcga
       61 atgctttggc aaaaagaatt aaagcacaac aaattcctct gaaatatgtt ataaatttcg
      121 acatgatagg aactacaatg atagatgctc ccaaaaaggt ttttataacc ggatttgata
      181 aatcgaattt agctgttact gcaaatacac tgttacaaga agattttttg ttaaaagatg
      241 cccaaaccga agatatggaa ctgttcagat attccgataa tttttcattt tatagagaac
      301 ttaatatacc cgcacatacc ttgagtacat ttaatatgag aaattttgac tgttatcata
      361 gaacatgcga tgaagtttcg gaaattgatg ttaaaaatct tgaatcaata attcaaaaaa
      421 gcaccacatt tattattgaa ttattaaaac aaaattcgca aattgcgttg aattaaaata
      481 tacaattaga tagaaagaaa taatgaaaca agaatgttca ttttgtggcc gtaaaaaaac
      541 agaagtcagc atgttgctta ccggtttgtc gggtaatatt tgtagtgact gtgttgggca
      601 agccatagaa attataaatc aagaattacc tgcggctaaa cgcaaatcaa ctaaaaaatc
      661 gaaaatatta aagccaattg agattaaaca acatctcgat gaatatgtaa tagggcaaga
      721 atcagcaaaa aaaatattgt cggtagcggt gtataaccac tacaaacgaa tttcgcaaga
      781 attgaccgat gatgttgaga ttgaaaaatc aaacataatc atggtgggcg aaacaggtac
      841 gggcaaaaca ttactggcaa aaacaattgc aaaaatgctc gatgttccgt ttgcaatagt
      901 agatgcaacg gtgctaaccg aagccggata tgttggtgaa gatattgaaa gtttgttggt
      961 tcgcttgcta caagatgctg actatgatgt accacgagcc gaacaaggta ttgtatttat
     1021 tgacgaaata gataaaatag ctcgcaaagg agacaacccg tctattactc gcgatgtgtc
     1081 gggcgaaggt gtgcaacaag gtctgttgaa attgctcgaa ggttcggtgg taaacgtgcc
     1141 accacaaggc ggtagaaaac accccgacca aaaaatgata gccgtaaata cgaaaaatat
     1201 actgtttatt tgcggtggtg cattcgaagg gattcaaaaa gttatagcac gacgtttaaa
     1261 tactaaggtt gtaggatttg gtgcaaaaga aaaaacacag caaatcgata aaaataattt
     1321 cttgcaatat atagcaccac aagatttacg aacatttgga ttgatacccg aaattattgg
     1381 tcgcttgcct gtgcttacct acctcaatcc attggataga gatgcattac gcagaatact
     1441 taccgaacct aaaaattcac tcattaaaca atataaagcg ttatttcatt tagataatat
     1501 tgaattgagt tttgaagaag aaacctacga ttatatagta gacaaagcta tagaatttaa
     1561 actcggtgct cgcggattac ggtcgatttg cgaagctatt atggttgatg ctatgtttga
     1621 atatccttca aaaaacataa aagaac
//
LOCUS       DAOTOW010000575         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_58058_length_1644_cov_4.842039, whole genome shotgun sequence.
ACCESSION   DAOTOW010000575 DAOTOW010000000
VERSION     DAOTOW010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58058_length_1644_cov_4.842039"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /gene="ccsA"
                     /locus_tag="P5243_09370"
     CDS             <1..302
                     /gene="ccsA"
                     /locus_tag="P5243_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRS19702.1"
                     /translation="GIAENIIYIGFGFLTLGLLFGALWAKEAWGHYWTWDPKETWALL
                     TWGVYIVYMHLSLQNRKSPRYYLWFITLSFTVLLIAWFGINYLPAAQMSVHVYSN"
     gene            455..1438
                     /locus_tag="P5243_09375"
     CDS             455..1438
                     /locus_tag="P5243_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS19703.1"
                     /translation="MNNIKKHILSPEFITALLVGFLLTIVQYKVARPMLMAERFVQGA
                     GWIQIVAAMIYAAWVMHYIKQPHLSATWRLRIWMTFSIVFFSQLLIGLLGAEQFLMTG
                     KLHFPIPALIIAGPLYRGEITFMIILFISTIVLTGPAWCSYLCYFGAIDGWASRGKTP
                     HTPIAHTWRYKITGLLLVIGVTIVLRICTVPILYAIILASLFGIGGLAVIVFISKKRK
                     KMVHCITYCPIGTIVSFAKYVSPFRMRIAPDCNACMKCSMTCKYDALQKIEIQHLRPG
                     LTCTYCGDCIPSCKSQSIHYTVFGSGTNKARIIFLVLTISLHTCCLMLARI"
BASE COUNT          482 a          292 c          324 g          546 t
ORIGIN      
        1 tgggtattgc cgagaatatt atttacatag gatttggatt tttgacattg ggtttgctgt
       61 ttggggcatt gtgggcaaaa gaagcgtggg gacattattg gacatgggac ccgaaagaaa
      121 catgggcatt gctcacatgg ggtgtgtata tagtgtatat gcatctgagt ttgcaaaatc
      181 gcaaaagtcc tcggtattat ctgtggttca taacactttc gtttactgtt ttgctcattg
      241 cttggtttgg cataaactat ttacctgcag ctcaaatgag tgtacatgtg tattcgaatt
      301 aagatgccac gctagcctgt cttttttagc cactaagtca caaaggcaca aagaaatgaa
      361 ataagaaaaa tactattcct tttagtcttg gagccttggt ggtaagaata attttaatag
      421 ccactaagac acaaaggcac taagaaaaat acacatgaac aacattaaaa aacacatact
      481 atcaccggaa tttataacag ccttgttagt tggatttcta ttaacaattg tacagtataa
      541 agtagcacgt cctatgctca tggcagaacg gtttgtacaa ggagccggct ggattcaaat
      601 agttgcggca atgatttatg cagcttgggt aatgcattat ataaagcaac cacatctttc
      661 tgccacctgg cgattgcgaa tttggatgac attttctatt gtattttttt cacaattact
      721 cataggatta ttgggagctg aacaatttct tatgaccggc aaactgcatt ttcccatacc
      781 ggcacttatt attgcaggac cattgtaccg cggcgaaata acgtttatga ttattttatt
      841 tatttcaacc atagtactta ccggaccggc ttggtgtagt tatttgtgtt actttggagc
      901 catagacgga tgggcttcgc gaggtaaaac acctcataca cccatagcac acacatggcg
      961 atacaaaata acaggattac tactcgtaat aggggtaact attgtattac gcatatgcac
     1021 tgtacctata ctgtatgcta taatccttgc aagtttgttt ggtattggcg gacttgcggt
     1081 aatagttttt atttcgaaaa aacggaaaaa aatggtacac tgtatcacct attgccctat
     1141 aggcaccata gtatcgtttg ccaaatatgt atcgccgttt cgcatgcgca tagcacccga
     1201 ctgtaatgct tgtatgaagt gtagtatgac ttgtaagtat gatgcgttac aaaaaattga
     1261 gattcaacat ctgcgtccag gacttacctg cacctattgt ggtgattgta taccttcgtg
     1321 taaatcacag tcaattcatt acaccgtatt tggttccgga accaacaaag cacgcatcat
     1381 attcctagtg cttactataa gtttacacac ctgttgtttg atgttggcga ggatataaat
     1441 tgggattatc ctattcaatt aatagtttga ctttttcaaa gtcttctatt gattcaatat
     1501 tgtttacata gatttttgtc ttaatagcag gtttatttct ttcaataatt gaattaattt
     1561 cctttttgtc atttgaatct gatatgtata atcactgttg tccgataaaa aattaagaga
     1621 aggaagatgt tagtcttcct ttct
//
LOCUS       DAOTOW010000576         1640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_58289_length_1640_cov_2.519243, whole genome shotgun sequence.
ACCESSION   DAOTOW010000576 DAOTOW010000000
VERSION     DAOTOW010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58289_length_1640_cov_2.519243"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="P5243_09380"
     CDS             complement(<1..573)
                     /locus_tag="P5243_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19704.1"
                     /translation="MNKKLMILLLLTTTVISFNSFGQKEQVNGKKVYVQNVSDLMFQL
                     SETIDTANNAFNNGVKTTYFFNYAEMIHIDLSNNYGMITGLSIRNVGMKTFDETIGNI
                     NYDKVKRRVFALGGSVAFKAGSFSKHSFIYAGCEYEIAFHYRQKLYDNFGKIIKQGEW
                     LSTATNRFLPSTFIGYQLPSGFNIQMKYYLN"
     gene            complement(611..>1640)
                     /locus_tag="P5243_09385"
     CDS             complement(611..>1640)
                     /locus_tag="P5243_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRS19705.1"
                     /translation="NNKRFAELYVDRTWNTLLPIQYYVESLSDTCNFELGIQNQTTFP
                     YDIMKLRDATRPYKILKQIPFGNTFTKDLAIEIIDKEKLGKDDITDFISIVFSATQEI
                     GNKFGALSKEIEDTYVRLDYELMFFINYLEETIGKDNFLLVLTSNHGVAYSSKYSMMK
                     NAESGIFKHVESMYLIDKYLDTMYGNGDWILHYNAQQIYLNHATIEKKHIPLEEIQRT
                     AADFILQLSGIQQVMIASDLQKNSFCDNGMENKIDNSFYQGRSGDIFIQLKPGWNEQF
                     TDVVAGHVSGYTYDSHVPLLWYGAKLLPETIKRTVYIPDITVTLCNIVNIPLPNMATG
                     SIISELFD"
BASE COUNT          550 a          275 c          187 g          628 t
ORIGIN      
        1 attcaaataa tatttcattt gaatattgaa tcctgatggt aattgatatc caataaaagt
       61 tgaaggtaaa aagcgatttg ttgcagtact caaccactct ccttgtttta ttattttacc
      121 aaaattatca tacaattttt gacgataatg aaaagctatt tcatattcac agccggcata
      181 tataaagcta tgtttgctaa atgaccccgc cttgaaagct accgaaccac ccaatgcaaa
      241 aaccctgcgt tttactttat cgtaatttat atttcctata gtttcatcaa atgttttcat
      301 accaacatta cggattgata atcctgtaat cattccataa ttattagaca aatctatatg
      361 tatcatttcg gcataattaa aaaaataagt ggtttttact ccattattaa aggcattatt
      421 agcagtatca atagtttcac tcaattgaaa cattaaatca cttacatttt gcacatacac
      481 ttttttaccg ttaacttgtt ctttttgtcc aaaggaatta aaactaatta cagtagtagt
      541 tagtagtaac aatatcatta attttttatt catattaatt attttttagt atttatattc
      601 tgttctataa ttaatcaaac aattctgaaa ttatagaacc tgttgccata ttgggtaaag
      661 gaatatttac aatattacat aaagttacag ttatatcggg tatatacaca gttcttttaa
      721 ttgtttcggg taataatttt gctccatacc acaacaaagg cacatgcgaa tcgtaggtat
      781 aacctgatac atgtcctgct accacatcag taaattgttc attccaaccc ggttttaact
      841 gtataaaaat atcacctgat ctaccttggt aaaatgaatt atcaatttta ttttccattc
      901 cattatcaca aaaactattt ttttgcaaat cagatgctat cattacctgc tgtataccac
      961 tcaattgtaa tataaaatct gcagctgttc gctgaatttc ttctaatggt atatgttttt
     1021 tctcaatagt tgcatgattc aaatatattt gttgtgcgtt ataatgcaaa atccaatctc
     1081 catttccata cattgtatct aaatatttat ctattaaata catagattct acatgtttaa
     1141 atattcccga ttctgcattt ttcatcatac tgtattttga tgaatatgca acaccatgat
     1201 tagatgttag caccaataaa aaattatctt tcccaatagt ttcttctaaa taatttataa
     1261 agaacatgag ttcataatct aatcgcacat aggtatcttc tatttctttt gaaagagcgc
     1321 caaatttatt tccaatttct tgagttgccg aaaacactat acttataaaa tcagttatat
     1381 catcttttcc taatttttct ttatcaataa tttcaattgc taaatcttta gtaaatgtat
     1441 ttccaaatgg tatttgttta agtattttat acggacgagt agcatcacgt aatttcataa
     1501 tatcatatgg gaacgttgtt tgattttgaa taccaagttc aaaattacac gtatcactta
     1561 atgattctac ataatactgt attggtaata atgtattcca tgttctatca acatataatt
     1621 ctgcaaaccg cttattatta
//
LOCUS       DAOTOW010000577         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_58600_length_1634_cov_3.529449, whole genome shotgun sequence.
ACCESSION   DAOTOW010000577 DAOTOW010000000
VERSION     DAOTOW010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58600_length_1634_cov_3.529449"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="P5243_09390"
     CDS             <1..474
                     /locus_tag="P5243_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS19706.1"
                     /translation="LVFLILAAQFDSFRDPLIIMISVPLAIAGGLLSLWIFGQTINIF
                     SEIGMITLVGLVTKNGILIVEFANQQREQGKTKFDAVIEAATLRLRPILMTSFATILG
                     ALPIALSLGSAGTSRMSLGIVIVGGLLFSLILTLFVIPAIYTYISGHTSKNTQSK"
     gene            471..>1634
                     /locus_tag="P5243_09395"
     CDS             471..>1634
                     /locus_tag="P5243_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS19707.1"
                     /translation="MKHISLLLIFLVSLLCTYAQKALSLEEAILFSVVSNYNVRIAKK
                     NVTQAELANNSAMAGALPHVNITGNAIVSDFIDDNRDLLFQNSLNADITVFNGFKIIA
                     TKEQLQLLQNQSKIALKIQIQNTIAAVIELYYEVLRQQSYIEILQASLAVAQERLAIV
                     TKRQQVGLANDSDVLQAQIDVIAIEQQISQQRTLIAQTKFELLYTMNKTEETDIVLTD
                     SITVGKLPPYDEIITKINTIPQILYSQIATEIAKTSARQIESQRYPSAKLVSNYTLQN
                     NTKSNSVVPMVGITYTIPLYSGGTVKTQMAIAQNSIAIKELEQAQAQSQILYHINKTY
                     VLYEQTLQQLHKQIDAYEYSKQLLHLVMKRFEANLATTLEVKAAQLSFEQAGYL"
BASE COUNT          601 a          297 c          247 g          489 t
ORIGIN      
        1 cttgtatttc tcatattagc tgcacaattc gacagtttta gagaccctct cattataatg
       61 atatctgtac cacttgctat tgccggagga ttactgtcat tgtggatatt tggtcaaaca
      121 atcaatattt tttcagaaat aggaatgatt actcttgtag ggttagttac caaaaatggt
      181 attttaatag tagaatttgc caatcaacaa cgagaacaag gaaaaaccaa atttgatgca
      241 gtaatagaag cagcaactct caggcttcga cctatattaa tgactagttt tgcaacaatt
      301 ttaggggcat tacctattgc tttaagtctt ggttctgccg gcaccagccg catgtcgttg
      361 ggtattgtta ttgtaggagg attactattt tctttaatcc ttacactatt tgtaatacct
      421 gctatttata catatatttc gggtcataca tctaaaaaca cgcaatctaa atgaaacaca
      481 ttagtttact acttattttt ttagtttctt tattgtgcac atatgctcaa aaagcattat
      541 cacttgaaga agctatactt ttttctgtag tttcaaatta taatgtacga atagcaaaaa
      601 agaacgtaac tcaggcagaa ctagcaaata attctgcaat ggcaggagct ttgccacacg
      661 taaatattac cggaaatgca attgtatcag attttattga cgacaataga gatttactgt
      721 ttcaaaactc actcaatgcc gatattactg tatttaatgg atttaaaatt atagcaacta
      781 aagaacagct tcaactactc caaaaccaaa gtaaaattgc actgaaaata caaatacaaa
      841 atactattgc ggcggtaata gaattatatt acgaagtact acggcaacaa tcgtatatag
      901 aaattttaca agcatcattg gctgttgctc aagaacgttt ggctatagta actaaaagac
      961 aacaagtagg attggccaat gattctgatg tattgcaagc tcaaatagac gtaatagcaa
     1021 ttgaacaaca aataagtcaa caacgcacac tcatagcaca aactaaattt gaacttttgt
     1081 ataccatgaa taaaaccgaa gaaactgata ttgtattaac cgattctatt actgtaggta
     1141 aacttccacc gtacgatgaa atcattacaa aaataaacac tattccacaa attctctatt
     1201 cacaaattgc tactgaaatt gctaaaactt cggctcgaca aatagaatcg caacgatacc
     1261 caagcgcaaa attggtttca aattatacgc tacaaaataa cactaaatca aatagtgtag
     1321 ttcccatggt aggtattacc tatactattc ctttgtattc gggcggcaca gtaaaaacac
     1381 aaatggctat tgcacaaaac tctattgcaa taaaagaatt agaacaggca caagcccaat
     1441 cacaaatatt atatcatatt aataaaacat acgtactcta tgaacaaaca ctacaacaat
     1501 tacataaaca aattgatgca tacgaatatt caaaacaact gctacattta gtaatgaaac
     1561 gatttgaagc aaatttggct actacgcttg aagtaaaggc agcacaactc agttttgaac
     1621 aagccggtta tttg
//
LOCUS       DAOTOW010000578         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_58986_length_1627_cov_2.561069, whole genome shotgun sequence.
ACCESSION   DAOTOW010000578 DAOTOW010000000
VERSION     DAOTOW010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58986_length_1627_cov_2.561069"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..536)
                     /locus_tag="P5243_09400"
     CDS             complement(15..536)
                     /locus_tag="P5243_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRS19708.1"
                     /translation="MALIKSVRGFTPKIGKNCYLAENATIIGDVVIGDECSIWFNAVL
                     RGDVNSISIGDKVNIQDGAVLHTLYEKSKIHIGNNVSIGHNVTIHGATIRDYVLVGMG
                     AIILDHAVVNENTIIAAGALVRDSEVLEPNSIYAGVPAKKIKEIDPEQTREIIIKIAN
                     NYSMYASWYTEGE"
     gene            complement(540..1391)
                     /locus_tag="P5243_09405"
     CDS             complement(540..1391)
                     /locus_tag="P5243_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRS19709.1"
                     /translation="MIQSVLEILDISDKKREIFWQDTQIIEAPNWSKDGFLLVNSEGR
                     LYKLYFDNKRKELVDTGFAINCNNDHGITPDGTRIIISSHDVDDANPMAWQTSKIYTL
                     PIQGGTPRLVTPKNGCFWHGISPDGNTHIYTALRNNQWDIYAISDDGGDEVQLTNSSV
                     QDDGSEFSPDGAYIYYNSFVSGSMEIWRMCADGSEQIQITNDDYSNWFPHICPNNRYM
                     VFLSYIQDQQQAHPFGQQVKLRCMNLSTKEIYDITDEFYGGQGTINVNSWSPDGTKLA
                     FVRYIKK"
     gene            complement(1394..>1627)
                     /locus_tag="P5243_09410"
     CDS             complement(1394..>1627)
                     /locus_tag="P5243_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19710.1"
                     /translation="RQAMYTNPYMKVWMANGLYDLATSYFGTQYFVNHMNLSPELQKN
                     ISMTYYKGGHMMYFVESELKQLKEDAVKLYQAK"
BASE COUNT          480 a          320 c          244 g          583 t
ORIGIN      
        1 tacatatttt atgtttattc cccctctgta taccaacttg catacataga ataattattc
       61 gctattttaa taataatttc acgagtctgt tcggggtcta tttctttaat tttttttgca
      121 ggcactccgg cgtatatgct attgggttcc aacacttcgc tatcgcgcac caatgcacct
      181 gcggcaatta tggtattttc gtttactacc gcatggtcga gaataattgc ccccatgcct
      241 accaaaacat aatctcttat ggtagctcca tgtatcgtta cattgtgccc tatagacaca
      301 ttgtttccaa tatgtatttt tgatttttca tataatgtat gcaacactgc accatcttga
      361 atattaactt tatctcctat tgaaatagaa tttacatcac cacgcaatac agcgttaaac
      421 caaatactac attcgtcgcc tattactaca tcgcctatta ttgtagcatt ttctgctaaa
      481 taacaatttt ttcctatttt gggtgtaaat ccacgaactg attttataag tgccataatt
      541 tattttttta tatatcgtac aaacgctaat tttgttccat caggtgacca cgaattgaca
      601 tttattgttc cttggccgcc ataaaattca tcggtaatat catatatctc tttggttgaa
      661 agattcatac aacgtagttt tacttgttgt ccaaacgggt gtgcttgctg ttggtcttgt
      721 atatatgaca aaaacaccat atacctatta tttggacaaa tatgcggaaa ccaattagaa
      781 tagtcatcgt tggttatttg tatttgttca cttccatcgg cacacatacg ccaaatttcc
      841 atactgcccg aaacaaacga attataataa atatacgcac catcaggcga aaattcagaa
      901 ccatcgtctt gtaccgaaga attagttagc tgtacttcat ctcctccatc atctgaaatt
      961 gcataaatat cccattgatt gttgcgtaat gctgtataaa tatgtgtatt gccatctgga
     1021 gaaatgccat gccaaaaaca tccattttta ggtgttacaa gtcgcggtgt gcctccttga
     1081 ataggtaaag tgtaaatttt tgaagtttgc catgccatgg gatttgcatc atcaacatca
     1141 tgcgaactta ttataattcg agttccatcg ggtgttatgc catggtcatt attacaattt
     1201 atagcaaaac cggtatcaac caattctttg cgtttattgt caaaataaag tttatacaat
     1261 cggccttcag aatttactaa caaaaaacca tctttgctcc agttgggagc ttcaattatc
     1321 tgtgtatctt gccaaaaaat ttctcgtttt ttatcagaaa tatcaaggat ttcaagtact
     1381 gattgtatca taattatttt gcttgataaa gttttactgc atcttcttta agttgtttta
     1441 attccgattc tacaaaatac atcatatgtc cgcctttgta atatgtcatg gaaatatttt
     1501 tttgtaattc gggcgaaaga ttcatatgat ttacaaaata ttgcgtaccg aaataactgg
     1561 tagctaaatc atataaacca ttagccatcc acactttcat atacggattt gtatacattg
     1621 cttgtcg
//
LOCUS       DAOTOW010000579         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59193_length_1623_cov_3.433036, whole genome shotgun sequence.
ACCESSION   DAOTOW010000579 DAOTOW010000000
VERSION     DAOTOW010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59193_length_1623_cov_3.433036"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="tsf"
                     /locus_tag="P5243_09415"
     CDS             complement(<1..721)
                     /gene="tsf"
                     /locus_tag="P5243_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRS19711.1"
                     /translation="MTQISATEVAKLRKITGAGMMDCKQALSESNGDFDKAIEFLRKK
                     GQKIASKREDRDANEGCVLGGVTADAKSACIVVVNCETDFVAKNEDFIAFTKSVLEVA
                     LQEKPANLEALKACKIKGEFISDLITDQMGKIGEKIDLSYYGSISAEHCFAYIHPGNK
                     LSTIVGFNKKVDEDQVGKDVAMQIAAMNPVSIDKDDCPTDIIEKEIEIGKEQAINEGK
                     PAEMAEKIAIGKLNKFYKESTL"
     gene            complement(737..1522)
                     /gene="rpsB"
                     /locus_tag="P5243_09420"
     CDS             complement(737..1522)
                     /gene="rpsB"
                     /locus_tag="P5243_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRS19712.1"
                     /translation="MARAEFNELLEAGVHFGHLKRKWHPGMAPYIFMERNGIHIIDLH
                     KTVAKIDESAEALKKIARSGRKVLFVATKKQAKEVVAQKVQSISMPYITERWSGGMLT
                     NFPTIRKAVKKMSSIDKMEADGMFSSLSKREKLQISRQREKLEKNLGSIVGLTRLPAA
                     LFVVDIVREHIAVNEARRLNIPVFAIVDTNANPDLVDFAIPGNDDASKSIEVILSKIC
                     DAIQEGLNERKLEKEKEVAQAEKTESTVEKTAPKKAAARKSKQ"
BASE COUNT          467 a          304 c          211 g          641 t
ORIGIN      
        1 acaaagtact ttctttatag aatttattta acttaccaat agctattttt tcagccattt
       61 ctgcaggttt tccttcattt atagcttgtt ctttacctat ttcgatttct ttttcaataa
      121 tatcagtcgg acaatcatct ttatctattg aaacaggatt catagcagct atttgcatag
      181 caacatcttt tccaacttga tcttcatcaa cttttttatt gaatcctaca attgtagata
      241 atttattgcc cggatgtatg taagcaaaac aatgttctgc agaaatagaa ccatagtaac
      301 ttaaatcaat tttttctcca attttaccca tttgatcggt aattaaatca gaaatgaatt
      361 cacctttaat tttacatgct tttaatgctt ctaaatttgc aggtttttct tgtaatgcaa
      421 cttcaagtac agattttgta aatgctataa aatcttcatt tttagcaaca aaatctgttt
      481 cgcaatttac tacaacaata caagctgatt ttgcatcggc agtaacacca ccaagtacac
      541 agccctcatt tgcatctcta tcttcacgtt tactggcaat tttttgtcct tttttacgta
      601 aaaattctat tgctttatca aaatcaccat tactttcgct taatgcttgt ttacagtcca
      661 tcattccggc acctgtaatt ttacgaagtt ttgcaacttc tgtagcagat atttgtgtca
      721 tacttttttc taaattttat tgttttgatt tacgagctgc tgcttttttt ggagcagttt
      781 tttcaacagt tgattctgtt ttttcagctt gagcaacttc tttttctttt tctaattttc
      841 gttcatttaa accttcttga attgcatcac aaattttact tagaattact tctatagatt
      901 ttgatgcatc atcatttccc ggaattgcaa aatctactaa atcaggattt gcgttagtat
      961 caactattgc aaatacagga atatttaatc tgcgagcttc atttactgct atatgttcac
     1021 gaacaatgtc aacaacaaac aatgctgcag gcaaacgagt taaaccaaca atactaccta
     1081 aatttttttc taatttttca cgttgacgag atatttgtag tttttcacgt tttgatagtg
     1141 aactaaacat tccatctgct tccattttat caatagaaga cattttttta acagccttac
     1201 gaatagtagg aaaattcgtt aacataccac cactccaacg ttctgtgata tatggcatgc
     1261 ttatactttg aactttttga gctacaactt cttttgcttg tttttttgtt gctacaaata
     1321 aaactttacg acctgaacga gctatttttt ttagtgcttc agccgattcg tcaatttttg
     1381 caactgtttt gtgtaggtct ataatgtgaa ttccattacg ttccatgaaa atgtagggag
     1441 ccattcccgg atgccatttt ctttttaagt gtccaaaatg aacacctgct tctaataatt
     1501 cattaaattc tgctcttgcc atgttatttt tgttgtttgc attcaattaa agcaatctgt
     1561 aagtagcacc ttatggtagt cttacaaatt tagagtataa acaacgctct aaatcgaata
     1621 tat
//
LOCUS       DAOTOW010000580         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59261_length_1622_cov_2.525207, whole genome shotgun sequence.
ACCESSION   DAOTOW010000580 DAOTOW010000000
VERSION     DAOTOW010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59261_length_1622_cov_2.525207"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="P5243_09425"
     CDS             <1..523
                     /locus_tag="P5243_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19713.1"
                     /translation="GINAEVLLGQWEFQIFAKGAKRAADEIWVARYLLERTCEKYGVY
                     VEWHPKPLGKDADWNGSGMHANFSNGKMRTCGDKKVFDEICEEFGKHIKEHMAVYGAY
                     NDQRLTGKHETASIHDFSYGVSNRGASIRIPVGTVEDGWKGRLEDRRPASNGCPYQIG
                     AIIVKSVKASGHI"
     gene            654..998
                     /gene="rbfA"
                     /locus_tag="P5243_09430"
     CDS             654..998
                     /gene="rbfA"
                     /locus_tag="P5243_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRS19714.1"
                     /translation="MDSTRQQKVSRLLLKELADIFQKESVTMFGGALISVTIVRVAPD
                     LSFAKVYISIFVPGGNTAAIFSLVQQHTKKIRSMLASRIAKQLRIVPELVFAIDDSLD
                     YIEHIDNLLHSK"
     gene            999..>1622
                     /locus_tag="P5243_09435"
     CDS             999..>1622
                     /locus_tag="P5243_09435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS19715.1"
                     /translation="MHYDPIKRTLGRVFNSTPVLRKLFYFLLNVLLLRSWHIRRRIKQ
                     WAVSAPHNASILDAGAGFGQYVFFMSQLNPHWFIMGVDVKQEQVEDCNTFFSKIGKSK
                     QIRYTEADLTQYVSTNPVDLIVCVDVMEHIEDDMVVFRNFSKSLKTGGLLLISTPSDL
                     GGSDVHDEHDESFVGEHVRDGYNKDEITQKLLQAGFSSVTSYDSYDFW"
BASE COUNT          520 a          226 c          321 g          555 t
ORIGIN      
        1 aggtataaat gctgaagtat tattgggtca atgggaattc caaatttttg caaaaggtgc
       61 taaacgtgct gctgatgaaa tttgggttgc tcgttattta ttggaaagaa catgcgaaaa
      121 atatggtgtg tatgtagaat ggcatccaaa accacttggt aaagatgcag actggaatgg
      181 ttctggtatg catgcaaact tctcaaacgg aaaaatgaga acatgtggtg ataaaaaagt
      241 atttgatgaa atttgtgaag aattcggaaa acatattaaa gaacatatgg ctgtgtatgg
      301 cgcttacaac gatcaacgtt taacaggtaa acatgaaaca gcatctattc acgatttctc
      361 atatggtgtt tcaaaccgtg gtgcttctat tcgtattcct gtaggaacag tagaagatgg
      421 atggaaaggt cgtttagagg atcgtcgtcc ggcatcaaat ggttgtcctt accaaatagg
      481 tgcaataatt gtaaaatcag ttaaggcttc aggtcacatt taatataaat gtattattgt
      541 tcgaaaacct tcggagagct ctcttcgaag gtttttttgt tattataagt tttctaaata
      601 atgaatccaa tataaattat ttgtatgttt gtataaatta aaatcaaata ttcatggatt
      661 caactcgaca acaaaaggtt tctcgattat tacttaaaga attagcagat atttttcaaa
      721 aagaatcggt tacaatgttt ggaggagcat taatttctgt aactatagtt agggttgctc
      781 ccgatttgtc ttttgctaaa gtgtatataa gtatttttgt gccgggagga aatactgcag
      841 ctattttttc attggtgcaa cagcatacta aaaaaatccg ttcaatgtta gcctcgcgta
      901 ttgctaaaca attgcgtata gtccctgaat tagtatttgc aatagatgat tcacttgatt
      961 atatagaaca tattgataat cttttacatt ccaaataaat gcattacgat cctataaaac
     1021 gtactttggg tagagtgttc aatagtactc ccgtattacg aaaattattt tattttttgc
     1081 tcaatgtatt attgttgcgt tcatggcata tacgaagacg tattaaacaa tgggcagttt
     1141 ctgctcccca caatgcttca attcttgatg caggtgcggg ttttggtcaa tatgtttttt
     1201 ttatgtcgca attaaatccc cattggttta ttatgggagt tgatgtaaaa caagaacaag
     1261 ttgaagactg taatactttt ttttcaaaaa tcggaaaatc aaaacagata cgttatacag
     1321 aggccgattt aactcaatat gtgtcaacaa atccggtaga tttaattgtt tgtgttgatg
     1381 taatggaaca tattgaagat gatatggttg tatttagaaa ttttagcaaa tcgcttaaaa
     1441 caggtggtct tttacttatt tctactcctt ctgatttggg aggtagtgat gtgcatgatg
     1501 aacacgatga atcttttgtt ggtgaacacg tacgtgacgg gtacaataaa gatgaaataa
     1561 ctcaaaaatt attacaggca ggttttagtt ctgttacatc atatgattca tatgattttt
     1621 gg
//
LOCUS       DAOTOW010000581         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59478_length_1618_cov_3.582853, whole genome shotgun sequence.
ACCESSION   DAOTOW010000581 DAOTOW010000000
VERSION     DAOTOW010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59478_length_1618_cov_3.582853"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="P5243_09440"
     CDS             complement(<1..983)
                     /locus_tag="P5243_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19716.1"
                     /translation="MFRKNVLVFVVCLCLSYNGFAQDALKKADMAFAANEYSFAITYY
                     KSVFSKIDNDNQTKARIAYNIGLCNKRISKPLEAQLWFEKAIELKHQDPALFLHYADV
                     LRMNEQFDKAIEYYNQYKLLVPRDVLADKGIESCKMAKQWLKDPTEYIITNLTYLNSE
                     FIDYSPNYVWDGNKSSIYFSSSRANAMGDKTHGGSGQSFADIFVSHEDAKGAWSFPVP
                     LNDAINSEHEEGTPSYSLATKSMFFTRCTYQENAASMCKIMESKQVDKTWIKAETVHV
                     SDIKKDTSDYVHPAISKNGLALYFSSNRPGGYGGYDIWVCTRLSVQEPWGKP"
     gene            complement(1094..>1618)
                     /locus_tag="P5243_09445"
     CDS             complement(1094..>1618)
                     /locus_tag="P5243_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS19717.1"
                     /translation="IIVQNIWGMVIDSVTYSETWHSKTLRSAEGISLERLDYTKPAYL
                     ARNWQSASTLSGGMTPGCENSQIVGNESTSFSAEHTCITPNNDGDNDVLRIAYTSNGK
                     DKTVQLYVFSFDGMLVQHTINNQLLANSGIIVWDATNAASEIVPPGVYILLIEVLEQG
                     TRVFAKKMSCTVLY"
BASE COUNT          521 a          313 c          239 g          545 t
ORIGIN      
        1 ggtttacccc atggttcttg aaccgataat cgagtacaca cccatatatc atatccacca
       61 tatccaccgg gacgatttga agaaaaatat aaagctaatc cattttttga tatagccggg
      121 tgaacataat cggaagtatc ttttttaata tcgcttacat gaacagtttc tgcttttatc
      181 catgttttat ctacttgttt agactccatt attttgcaca tacttgcagc attttcttga
      241 tatgtacatc gagtaaaaaa cattgattta gtagccaacg aataacttgg tgttccttct
      301 tcatgttctg aattaattgc atcattcaga ggaacgggaa aactccatgc tcctttagca
      361 tcttcatgtg acacaaaaat atcggcaaaa ctctgcccac tgcctccatg cgttttgtcg
      421 cccattgcat tggcacgtga cgacgaaaaa tagatactac ttttatttcc gtcccataca
      481 tagttaggac tataatcaat aaactctgaa ttgagatagg taaggtttgt aattatgtat
      541 tctgttggat cttttaacca ctgttttgcc attttacatg actctattcc cttatcggca
      601 agcacatcac gtggtactaa taatttgtat tgattataat attctattgc tttatcaaat
      661 tgttcgttca tgcgcaatac atcagcataa tgcagaaata acgccggatc ttgatgtttt
      721 aattctatag ccttttcaaa ccataattga gcttcgagtg gttttgaaat acgtttatta
      781 cataatccta tattatatgc tattcgagct tttgtttgat tatcgttatc tattttacta
      841 aatacacttt tataataagt aatggcaaaa ctatattcgt tagcagcaaa tgccatatct
      901 gctttcttaa gagcatcttg tgcaaagcca ttataggaca aacacaaaca cacaacaaaa
      961 acaagcacat ttttacgaaa catagtattc attcaatgat ttataaaatc aaatatactg
     1021 ttagattttg acaaaccaaa acagacaatt aaaaaaaaca tatacaaatg taatataaag
     1081 atagtatttt caattaatat aataccgtgc aactcatttt tttcgcaaaa actcgagttc
     1141 cttgttctaa aacttcaatc aagagaatat aaactccggg aggaacaatt tctgaagcgg
     1201 catttgtagc atcccacaca ataatgcccg aatttgctaa caactgatta ttaatagtat
     1261 gttgcaccaa cataccgtca aatgaaaaaa catataattg cactgtttta tcttttccat
     1321 tggaagtata tgctattcgc aacacatcgt tatcaccatc attgttggga gttatacatg
     1381 tatgttctgc tgaaaaagat gttgattcgt ttccaacaat ttgtgaattt tcgcacccgg
     1441 gcgtcattcc gccacttaga gttgaagccg attgccaatt tctagcaaga tacgccggtt
     1501 tggtataatc aagcctttct aatgaaattc cttctgccga ccgtaaggtt ttagaatgcc
     1561 atgtttctga atatgttaca gaatctataa ccatacccca tatattttga acaataat
//
LOCUS       DAOTOW010000582         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59549_length_1617_cov_3.229834, whole genome shotgun sequence.
ACCESSION   DAOTOW010000582 DAOTOW010000000
VERSION     DAOTOW010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59549_length_1617_cov_3.229834"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="P5243_09450"
     CDS             complement(<1..1573)
                     /locus_tag="P5243_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRS19718.1"
                     /translation="MIPIVLVDQNGKPLDRKLKIEIYEIYWRWWWDMSEEENLADYIS
                     NQHSHIIKTDYVTTKNGKALYEMNLGGNYWGRKFIRVYDEESEHSTGATFYTTYKSWW
                     SNAGNENPGGAEMLMFNTDKKNYTVGEKVQVELPATHKGRALVSIETGSKVLQNFWVE
                     VTQENPKFSFTVTPEMSPNVYIHISYIQPHNKQNNDFPIRMYGVQNIKVDDPATRLEP
                     IITMSKELKPEQTFKVQVKEAKGKAMTYTIAIVDEGILDLTRFKTPNPWDAFYAQEAL
                     GVRTWDLYKYVAGAYTGKIAGLLAVGGDEFASKKGKENTNRFKPVVLFKGPYTCKAGE
                     TKTHEFTMPNYVGSVRVMVVAGNNGAYGNAEQTVPVKQDLMVLPTIPRVISPTEEITI
                     PVTVFAMQPSIKQVQVQLQVDKHITLLDGATRTVSFDKPGDKIVEFKVKAKEISAQTK
                     ITVLASSGTIQAKSETNLNIRLPNPPVTDAINIVLKPGETVQRAIQAIGIAGTNSGII
                     EISRVLPLNLKNHIQY"
BASE COUNT          374 a          322 c          297 g          624 t
ORIGIN      
        1 ggtattgtat gtgatttttt aaattgagtg gtaacacacg cgaaatttca attattcccg
       61 aattggttcc cgcaatgcct attgcttgta ttgctcgttg aacagtttct cccggtttta
      121 acacaatatt tatagcatcg gttactggcg gatttggaag acgaatattg agatttgttt
      181 ctgatttagc ttgtatagtt ccagacgaag caagtacggt aatttttgtt tgtgcactta
      241 tttctttcgc ttttacttta aactctacaa ttttatcgcc cggtttgtca aatgatactg
      301 ttcgggtagc tccgtcgagc agagttatat gtttatcaac ttgaagttga acttgcactt
      361 gttttatact cggttgcatt gcaaatacag ttaccggtat cgttatttct tcggtaggac
      421 tgattactcg tggaattgta ggcagtacca ttaaatcttg ttttaccggt acggtttgct
      481 ctgcattgcc gtatgcacca ttattccccg caactaccat tacacgtacc gaacctacat
      541 aattgggcat ggtaaattca tgtgttttgg tttcgcctgc tttacacgta tatgggcctt
      601 tgaataaaac aacgggttta aatctatttg tattttcttt accttttttg cttgcaaatt
      661 cgtcgccacc tacggcaagt aatccggcaa ttttaccggt ataggcaccg gctacatatt
      721 tgtacaaatc ccatgttctt actcccaatg cttcttgtgc ataaaatgca tcccatggat
      781 taggtgtttt gaagcgggta aggtccaata ttccctcgtc aactattgct atagtgtatg
      841 tcatagcttt accttttgct tcttttacct gcactttgaa tgtttgctca ggttttagtt
      901 ctttgctcat ggttattatt ggctctagcc ttgtagcagg gtcatctact ttaatatttt
      961 gtacaccata catgcgtatc gggaaatcgt tgttttgttt gttgtgcggt tgaatatatg
     1021 atatgtgtat gtatacattt ggcgacattt caggagttac ggtaaatgaa aatttcggat
     1081 tttcttgtgt tacttctacc caaaagtttt gtaatacttt actacctgtt tcaatactta
     1141 caagagctct tcctttgtgt gtggcaggta attctacttg tactttttcg cctacggtat
     1201 aatttttttt gtcggtatta aacattagca tttctgctcc acccggattt tcgtttccgg
     1261 cattgctcca ccaacttttg tacgtagtat aaaatgttgc tccggtgctg tgttcacttt
     1321 cttcgtcgta cactcgtata aatttgcgcc cccaataatt accgccaagg ttcatttcat
     1381 acaacgcttt tccgttttta gttgttacgt aatcggtttt aattatatgt gaatgttggt
     1441 tcgaaatata atcggcaaga ttttcttctt cgctcatatc ccaccaccag cgccaatata
     1501 tttcataaat ttcaatttta agttttctgt caagcggttt gccgttttgg tcaactagta
     1561 ctattggtat caagtttttc tcgtcagaat acaacgcatt gttccatcct tttcctt
//
LOCUS       DAOTOW010000583         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59567_length_1617_cov_2.681178, whole genome shotgun sequence.
ACCESSION   DAOTOW010000583 DAOTOW010000000
VERSION     DAOTOW010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59567_length_1617_cov_2.681178"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..440)
                     /locus_tag="P5243_09455"
     CDS             complement(66..440)
                     /locus_tag="P5243_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19719.1"
                     /translation="MKLHAQYKMSNKKYMVTLSLIKFKEDKAAIIYSPALDLSGYGNT
                     FEDAKKSFSTTLQEFIKYTHNKRTLETCLIKMGWKIKQGKQKVNFVPPKETDLIKKNS
                     LYTDIVNNKEYSVMRQNVEFAL"
     gene            complement(522..1073)
                     /locus_tag="P5243_09460"
     CDS             complement(522..1073)
                     /locus_tag="P5243_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19720.1"
                     /translation="MKRFIIELAICIACGMCAFALLQTCKHASNSIIVLDTDTITIVK
                     KDTVYKHSYTPTYIPVEHYYTDTVHRYDTVFIVTDYKEVRVYLDTIRDTSFVVYLRDT
                     VTQNKIVSRSYTGNSFSTTKYITNTIEAPQKLHIAIGPAVQYSNTIFSNPGILLQAHK
                     QKFGGGVSLHQKGVQLYGLFYLR"
     gene            complement(1070..>1617)
                     /locus_tag="P5243_09465"
     CDS             complement(1070..>1617)
                     /locus_tag="P5243_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19721.1"
                     /translation="TIDNSYKHIIEPLARINQTRKELITAVIFLESSGNAKAKTAYAV
                     GLMGLAPRSMTDTVTRERKRGKISEYEKEIIKKHVGASKFAKIMNAKMGDDFFTADEC
                     LIPELNICLGTMYLMQIVDEETKNGTVRLDRVIVRYNKGYYATIPSGSTQDLLNSNLP
                     YETKSYILKLAGINGILDILL"
BASE COUNT          491 a          262 c          258 g          606 t
ORIGIN      
        1 cttgttttgt taaaaaacta cgaaatgcag caacgcttat attttttaaa ctttgagtat
       61 tcatattata atgcaaattc tacattttgt cgcattaccg aatattcttt gttatttact
      121 atatcagtat acaggctgtt tttctttata aggtcagttt ctttaggagg aacaaaattt
      181 actttttgtt tgccttgttt aattttccac cccattttta ttaaacatgt ttctaacgtg
      241 cgtttattat gagtatattt tataaattct tgtaatgtag tagaaaacga ttttttggca
      301 tcttcaaacg tattgccata tcccgataaa tcaagagccg gagaatatat tatagctgct
      361 ttatcttctt taaattttat taacgataaa gttaccatat attttttatt agacatttta
      421 tattgagcat gtaatttcat aaaattataa ttttaaaact ctatacaaat atatgttgat
      481 ttattcaaaa ttgtatgttt ttacaaaaga aacatttcat tttacctcaa ataaaacagt
      541 ccatacaatt gaacaccttt ctgatggagc gacactcccc caccaaattt ttgtttatga
      601 gcttgcaaaa gtattcccgg attagaaaat atagtattgg aatattgcac ggcaggacct
      661 atagctatat gcaatttttg tggagcttct atggtattgg taatgtattt ggtagtgcta
      721 aatgaattac cggtatacga acggcttact attttatttt gagttaccgt atcgcgtaaa
      781 tacacaacaa aagaagtatc gcgtatagtg tctaaatata cgcgtacttc tttgtagtcg
      841 gtaacaataa aaacagtatc gtaccggtgt acggtatctg tatagtagtg ttcaaccggt
      901 atatatgtgg gggtatacga atgcttgtat acggtatctt tttttacaat ggtaatagta
      961 tcggtatcta acacaattat actgttgctt gcatgtttgc atgtttgcaa caaagcaaat
     1021 gcacacatgc cacaagcaat acaaatagct aattcaatta taaatcgttt cataagagta
     1081 tatctaaaat tccattgata cctgctaatt tgagtatgta tgattttgtt tcgtatggta
     1141 gattagaatt aagcaaatct tgtgtactac cacttggaat agtagcatag tagcccttat
     1201 tgtatcgtac aattactcta tcgaggcgta cagtaccgtt tttagtttcc tcgtctacta
     1261 tttgcattaa atacatggtg cctaaacaaa tattaagttc cggaatcaaa cattcatctg
     1321 ccgtaaaaaa atcatcgccc attttggcgt tcataatttt agcaaatttt gaagctccta
     1381 catgcttttt aattatttct ttttcatatt ccgatatttt accacgtttg cgttcgcgag
     1441 ttacggtgtc ggtcatagaa cgaggagcta accccattaa tccaactgca taggctgttt
     1501 ttgctttggc attgccacta ctttcgagaa aaataactgc agtaattaat tctttgcgtg
     1561 tttggtttat acgtgccagt ggttctataa tatgtttgta gctattgtca atagtat
//
LOCUS       DAOTOW010000584         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59605_length_1616_cov_3.836003, whole genome shotgun sequence.
ACCESSION   DAOTOW010000584 DAOTOW010000000
VERSION     DAOTOW010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59605_length_1616_cov_3.836003"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..391
                     /locus_tag="P5243_09470"
                     /pseudo
     CDS             1..391
                     /locus_tag="P5243_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008563749.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
     gene            complement(475..>1616)
                     /gene="glgP"
                     /locus_tag="P5243_09475"
     CDS             complement(475..>1616)
                     /gene="glgP"
                     /locus_tag="P5243_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRS19722.1"
                     /translation="LHKTWTGRFEDPKKLMKISQSLNKNTLTIGFARRFATYKRAYLL
                     FSNLERLSKIVNNPETPVQFIFAGKAHPNDKPGQDIIKEIVRISKMPEFLGKVIFLEN
                     YDMNLAKQLVQGVDIWLNTPTRPLEASGTSGMKAVMNGAMNFSVLDGWWCEGYKEGAG
                     WALPEKRTYDDQEFQNRLDAETIYSILENEIVPLYYNRGMNNIPHGWIQYMKKCIAEI
                     APEFTTKRMIDDYHERFYSKLAERIELVRKDDYKVAEEIVQWKKKIAEHWDSLYVVSC
                     EIAKTTDALTLGESYKGCVKVDLKGLAPHDVEFEFVLAETNKKGELEVIEKQRFVYKE
                     MDEAYAVYEVEFTPKQSGIFNYSVRMYPMHKYLPYRQDFPYIKWI"
BASE COUNT          540 a          286 c          220 g          570 t
ORIGIN      
        1 cgtttgacat tgaaaataaa accaaattat tagaaacatc tcaaacttgt tttgaagaac
       61 gttcaataga tttacaacaa aaacaaacag aattaaacga cattatttct gaaactcaaa
      121 aagaagagaa gaactaatag cagcttctga aaaattagca agcgttattg aagaccgatt
      181 attagcagca tataaacgca tacgttctaa tgcacgcaac ggattagctg tagcaaatgt
      241 agagcgcgat gcttgtggag gttgttttaa taaaattcca ccgcaacgac aactagatat
      301 aaaaacacac aaaaaaataa ttgtttgtga atattgtggt agaatactac ttgatgcaga
      361 attaataaaa agtgaattag aaaacgcata aaaaaaaagc tctcaatttt ttgggagctt
      421 tttttatgat taaaaaaaag gtgcaatata catattgcac cttttttatt ataattaaat
      481 ccattttata taaggaaaat cttgacgata tggtaaatat ttatgcatag gatacattct
      541 aacactataa ttaaatatac ctgattgttt tggtgtaaat tccacttcat aaacagcata
      601 cgcttcatcc atttctttat acacaaatct ttgtttttca attacttcga gttctccttt
      661 tttattggtt tctgcaagca caaattcaaa ttctacatca tgcggagcaa gaccttttaa
      721 atcaactttt acacatcctt tatatgattc tcctaaagtc aaagcgtcag ttgtttttgc
      781 aatttcgcat gacacaacat ataatgaatc ccaatgttca gcaatttttt tcttccattg
      841 cacaatctct tctgcaactt tataatcatc ttttcttact aattctattc tttcagcaag
      901 ttttgaataa aaccgttcat gataatcatc aatcattcgt ttagttgtaa attcgggagc
      961 aatttctgct atacattttt tcatatattg aatccaacca tgaggtatat tattcattcc
     1021 cctattatag tacaaaggaa caatttcatt ttcaaggata ctgtaaattg tttctgcgtc
     1081 taatctattt tggaattctt gatcatcgta cgtgcgtttt tcaggtaatg cccatccggc
     1141 tccttctttg taaccttcgc accaccaacc atcaagcaca ctaaaattca ttgcaccatt
     1201 catcactgct ttcattccgc tggtaccaga agcttctagt ggtcgagttg gtgtatttag
     1261 ccatatatca acaccttgca ctaattgttt tgcaagattc atatcataat tttctaagaa
     1321 aataactttt cctaaaaact caggcatttt tgaaattcta actatttctt taataatatc
     1381 ttgaccgggt ttatcatttg gatgagcttt acctgcaaat ataaactgta caggagtttc
     1441 aggattatta actattttac ttaacctttc aagattgcta aacaataaat atgctcgttt
     1501 atatgtagca aatcttcgag caaaacctat agttaaagta tttttattga gtgattgaga
     1561 gattttcatt aattttttag gatcttcaaa gcgccctgtc catgttttat gtaatc
//
LOCUS       DAOTOW010000585         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59618_length_1616_cov_2.770019, whole genome shotgun sequence.
ACCESSION   DAOTOW010000585 DAOTOW010000000
VERSION     DAOTOW010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59618_length_1616_cov_2.770019"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="P5243_09480"
     CDS             <1..1041
                     /locus_tag="P5243_09480"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS19723.1"
                     /translation="AKKAIDDNFSFYTPVEGFADLIQAICDKFKNENNLEYSPSQIIV
                     SAGAKHSLSNIFLSLLDPGDEVLIPTPYWVSYPEAIKLAEGKPVYIPSTIDSGFKVTP
                     EQIAKAITSKTKMFVFSSPSNPCGAVYTKEELQQLAKLFAKNPQILIVSDEIYEHINF
                     TGKHESIAQFKEVKNQVIVVNGVSKGYAMTGWRIGYMAAPEWIAKACNKLQGQVTSGV
                     CSIAQKASIAALRGGLEFPKMMTQAFLRRRNLMVQGLSKVKGMKIDTPQGAFYIFADV
                     SGLFGKKTPNGTTIKNSYDLALYLLSEAWVAAVSGEGFGNPECIRFSFATSDENLEKA
                     IARITEAVDKLQ"
     gene            1128..>1616
                     /locus_tag="P5243_09485"
     CDS             1128..>1616
                     /locus_tag="P5243_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRS19724.1"
                     /translation="MCGISGLFSFSHAASYYRAAIEQSLAVMKYRGPDAQAVFEHNAV
                     LLGHVRLSIIDTSFGANQPFTDNSGRYTIVFNGEIYNFQQFYTELQSDGVLFRTTSDT
                     EVLLYLYIKYGSECLQKIQGFFAFAVYDSHTNSLFVARDRMGVKPLYMYSNHSFVCFA
                     SEL"
BASE COUNT          524 a          263 c          292 g          537 t
ORIGIN      
        1 gcaaaaaaag ctattgatga taatttttcg ttttatacac ctgttgaagg ttttgctgat
       61 ttaattcaag caatatgtga taaatttaaa aatgagaata atcttgaata ttcgccatcg
      121 caaattatag tttcggcagg agcaaaacat tccttgtcta atatttttct tagtttatta
      181 gacccgggtg atgaagtgct tataccaaca ccatattggg ttagttatcc tgaagctatt
      241 aaattagccg aaggtaaacc cgtatatatt ccttctacaa ttgattcagg gtttaaagtt
      301 actcccgaac aaatagctaa agctattact tcaaaaacaa aaatgtttgt atttagttct
      361 ccttcaaatc cttgtggagc tgtatacacc aaagaagaat tgcaacaatt ggcaaaatta
      421 ttcgctaaaa atccgcaaat tctaattgtt tccgatgaaa tttatgaaca tattaatttt
      481 accggaaaac atgaatcaat agcacaattt aaagaagtaa aaaatcaagt aattgtagta
      541 aacggtgttt caaaaggata tgctatgacc gggtggagaa tagggtatat ggcagctccc
      601 gaatggatag caaaagcatg taataaatta caaggacaag taacttcggg tgtttgttca
      661 attgcacaaa aagcttcaat agcagcatta cgtggcggtt tagaattccc taaaatgatg
      721 acacaagcat ttttgcgtcg tagaaatctt atggtgcaag ggctatcaaa agttaaaggc
      781 atgaagatag atactccgca aggtgcattt tatatttttg ctgatgtgtc cggtttgttt
      841 ggcaaaaaaa caccgaatgg tactactatt aaaaattcat acgatttagc tctatattta
      901 ttgtcagaag cttgggttgc tgctgtttcg ggcgaaggat tcggaaatcc ggaatgtatt
      961 cgtttttcat ttgctacaag cgacgaaaat ctagaaaaag ctattgctcg tattactgaa
     1021 gcagttgata aactgcaata gatatacaaa ttacgttttt acaatactat cactataaaa
     1081 tccggtaaaa aagtgctacc tttctaccgg atttttttat taatactatg tgtggcataa
     1141 gtggtctttt ttctttttct catgcagcat cgtactatag ggctgctata gaacaatcac
     1201 ttgcagttat gaaatatcgt ggtcccgatg ctcaagctgt atttgaacac aatgcagtgt
     1261 tgcttgggca tgtccgctta tctattattg atacttcgtt cggtgccaat cagcctttta
     1321 ccgacaatag tggcagatac acaatcgtat tcaacggtga aatctataat tttcaacaat
     1381 tttatactga attacaatca gatggagttt tattcagaac tacttccgat accgaagtct
     1441 tactatattt atatattaaa tatggaagtg aatgtttaca aaaaattcag ggtttttttg
     1501 cttttgcagt ttacgattcg catacaaatt ctctattcgt agcacgagat agaatggggg
     1561 taaaacctct ctatatgtat tcaaatcatt catttgtttg ttttgcttcg gaatta
//
LOCUS       DAOTOW010000586         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59830_length_1612_cov_3.667951, whole genome shotgun sequence.
ACCESSION   DAOTOW010000586 DAOTOW010000000
VERSION     DAOTOW010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59830_length_1612_cov_3.667951"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="P5243_09490"
     CDS             complement(<1..369)
                     /locus_tag="P5243_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19725.1"
                     /translation="MGFSVRCLRDLKENIATGLFSCGFFKHKKMKQDNLFSEIDREKI
                     TLDLFKAYFDARKNKRNTINALAFEKHLEANLFALANDIIERKYIPNQSICFIVNKPI
                     KREIFAADFRDRVVHHLIIGK"
     gene            complement(414..821)
                     /locus_tag="P5243_09495"
     CDS             complement(414..821)
                     /locus_tag="P5243_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19726.1"
                     /translation="MKIKKFENIIAFSSVRKNVFCESSVSYGLPKRASLNPVTRWHYK
                     NLLCKKQVNVSNKRFHRLKMPVRQTHFVTKFKIIKFKFLRIDLNFSRVKRLLLQLFRV
                     ATVTIMEISTILETTVIGGVLPRTILIMHGTGT"
     gene            complement(1174..1521)
                     /locus_tag="P5243_09500"
     CDS             complement(1174..1521)
                     /locus_tag="P5243_09500"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS19727.1"
                     /translation="MALYNELPIYKAIYDLLLAIFKFTKDFSKEYKYTVGESLKKETI
                     ELLTLIYRANSRHQKDDVLQQAREQIEVIRLLIRVMKDMRQINIEKFVKINEVIENVS
                     KQLTGWQKSVKSL"
BASE COUNT          507 a          295 c          223 g          587 t
ORIGIN      
        1 tttaccgatg attaaatgat gtactactct atccctaaaa tcggctgcaa atatttctct
       61 tttaattggt ttatttacaa taaaacatat gctttgatta ggtatatatt tgcgttcgat
      121 aatatcattg gctaaggcga acaggttggc ttctaagtgt ttttcaaatg caagtgcatt
      181 tatagtattt cttttgtttt ttcttgcgtc aaagtaagct ttaaacaaat ctaatgtaat
      241 tttttctcta tctatttctg aaaataaatt atcttgtttc atttttttat gtttaaaaaa
      301 gccacaggaa aaaagacctg tggctatgtt ttcttttaaa tccctcaaac aacgaacaga
      361 aaaacccata ctcttgttat tgttgttcct atttacatta ctattattgt aattcaagtt
      421 ccggttccat gcattattag tattgttctc ggtagaactc caccaattac cgttgtttct
      481 aatattgttg aaatttccat tattgttacg gttgccaccc ggaagagctg taaaagtaac
      541 cgctttactc tgctaaaatt taagtcgatt cttaaaaatt tgaatttgat aatcttaaat
      601 ttagttacaa agtgagtttg tcgcacaggc attttcagcc tatgaaatct tttattgcta
      661 acatttacct gctttttaca aagtaagttt ttataatgcc agcgggttac cggatttaaa
      721 gatgctctct tcggcaaacc atagcttacc gaactctcgc agaaaacatt ttttcttaca
      781 ctgctaaaag caataatatt ttcaaatttt ttaatcttca taatttaaaa accattactg
      841 tttttattgt tttgccttac agaaaatcag ttaaaagtcg aaagtataaa gttaaaagtc
      901 gaaccgagct tgcgagctca ccgaaagtaa agtattgtgt tacccttgcg ggcaaggctt
      961 ttgagagact tgttttgtac tcacgtacaa cccacattag ctcactcaaa cgcccatgtc
     1021 gctcgctcca acaccgctga tgcttagtga ttgcaattac actcggatta caaccgaagt
     1081 ccgcaggacg agctcagcga agttaaatcc gagcgagcat acatcgtagt aacaaaaaaa
     1141 ttgctacttt acgaacttta aaaactttta actttataaa cttttaactg atttctgcca
     1201 tcctgttaat tgttttgata cattttctat tacttcattt atttttacaa atttttcaat
     1261 atttatttgt ctcatatctt tcattacacg tataagcaaa cgtattacct ctatttgttc
     1321 gcgtgcctgt tgaagcacat catctttttg atgcctcgaa ttagcacgat aaattaacgt
     1381 aagcaattct attgtttctt tttttaaact ttcacctact gtatatttat attccttgct
     1441 aaaatccttt gtaaacttaa atatagctaa aagcaaatcg taaattgctt tataaatagg
     1501 taattcatta tatagtgcca aatctcaaaa aaatttcgga cccgcaagcg ggtcattaac
     1561 aaataaatca aatagacaaa tagtcaaata aattaattag tccctcaaac aa
//
LOCUS       DAOTOW010000587         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59838_length_1612_cov_3.060373, whole genome shotgun sequence.
ACCESSION   DAOTOW010000587 DAOTOW010000000
VERSION     DAOTOW010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59838_length_1612_cov_3.060373"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..>1612
                     /locus_tag="P5243_09505"
     CDS             343..>1612
                     /locus_tag="P5243_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19728.1"
                     /translation="MKILPIILFLSIHCFNYAATIQSAASGNWGNTSTWVGGIVPTQN
                     DDIEIVSGHAITLNANAQINNIVITNGSIAIGSYTLQIFGSISGTQKNNVSSTANSTL
                     IIDDNGNAPQFTFPSNITKLKKLVMNRANGAETNQSLDLDDNVPADSIVLVLTNGILY
                     MNNESIFYMNSQAIKRNIPCSNASHIDGPVQRDIKKNSGMHVFPIGDNGMCRPMAIEA
                     QNGTNNINQAQFIYATPPNNLNVNTSKLPGGIFQYFYWDHQLISSANTQRRFYFQDSD
                     FPGISENDIANSFYLANSSGTTEWTIPTTPRTVDNTNNWISFSNSNASNDRYWTFGSN
                     NINVQIDNIILPIELIFFDASVNIGGIVLDWATASETNNEYFIVQRSVNGIDFTNIAT
                     INGAGNSYNTLFYSYVDDNVKPNYVYYYRLQ"
BASE COUNT          597 a          254 c          226 g          535 t
ORIGIN      
        1 tttttaagat ttcaatttta aacttacttt cttctgaatt agttatagga taagcatatg
       61 caataaactt acttcccttt tccttatatt ctcctattac cggcaattgt attgtaaggt
      121 aatgataaac atgcatataa atccattttg ctttcaaaat tataaaaatg tatggtgaac
      181 atggtaaatt ataatatata gttattaacg tgttatacac gaacatacac aattttcaat
      241 acaattgtaa atatcattaa taaactgaaa atcatatatt ttaattaaaa aaatataact
      301 tcactaatga atatttcgta taaaaaatta aaaaaataat atatgaaaat actaccaatt
      361 atattatttt taagcataca ttgttttaat tatgcagcaa ccattcaatc tgccgcttcg
      421 ggcaattggg gcaatacatc tacatgggta ggtggaatag taccaacaca aaacgatgat
      481 atagaaatag tttccggtca tgcaataaca ttaaatgcaa atgctcaaat caacaatata
      541 gtaattacta acggtagcat agcaattggc agttacacac tacaaatatt cggctcaatt
      601 tccggcacac aaaaaaacaa tgtaagttct actgcaaatt ctacattaat aatagatgat
      661 aatggaaatg ctcctcaatt tacatttcct tcaaacatta ctaaactcaa aaaattagta
      721 atgaatagag caaatggtgc cgaaacaaat caaagtcttg atttagatga taatgtacct
      781 gcagatagta ttgttttggt tttaacaaac ggtattttgt atatgaacaa cgaaagtatt
      841 ttttatatga atagccaagc tataaaacga aatatcccat gctcaaatgc ttcgcacatt
      901 gatggccccg tacaacgtga tattaaaaaa aattcaggta tgcacgtgtt ccctattggt
      961 gataatggca tgtgtagacc tatggcaatt gaagctcaaa atggaacaaa caatataaat
     1021 caggctcaat ttatttatgc aactcctcct aataatttaa atgtaaacac gtcaaaatta
     1081 cccggtggta tatttcaata tttttattgg gatcaccaac ttatatcaag tgccaatact
     1141 caacgccgct tttattttca agattcggat tttccgggaa taagtgaaaa tgatattgca
     1201 aattcttttt acttggcaaa ttcatcgggc actaccgaat ggacaatacc taccactcct
     1261 cgtactgttg ataatacaaa taactggatt tcattttcaa attcaaatgc ttcaaacgac
     1321 agatattgga catttggttc caataatatc aatgtgcaga tagataatat tattttacca
     1381 atagaactca tattttttga tgcatcggta aatattggcg gtatagtttt agattgggct
     1441 acagcaagtg aaactaataa tgaatatttt attgttcaac gctctgtaaa tggtatagat
     1501 tttacaaata ttgcaactat taatggtgca ggcaatagtt acaacactct gttttattca
     1561 tatgttgatg ataatgtaaa accaaattat gtatattatt atagattaca ac
//
LOCUS       DAOTOW010000588         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_59848_length_1612_cov_2.642261, whole genome shotgun sequence.
ACCESSION   DAOTOW010000588 DAOTOW010000000
VERSION     DAOTOW010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59848_length_1612_cov_2.642261"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1509
                     /locus_tag="P5243_09510"
     CDS             109..1509
                     /locus_tag="P5243_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRS19729.1"
                     /translation="MSHSSKKLFKHAAERIAFDEQHRKTIQFNMGKYHAAVGAGKLRY
                     ANLDAARIKASEIKRHTLANLPQYLEEFEKNCIQNGITVLWAQTAADAIQYITNILQE
                     HKVTSVVKSKSMTTEEIELNHELEKIGIESVETDLGEYIVQLAGEKPYHIVTPAMHKS
                     KQDVAVLFHQKFGFPLTSTAEEMTNLARQKLREKYLAAGAGITGANFLIADKGGVCVT
                     ENEGNAIMSTAFPDIHIAIAGIEKVIPSFKDLALFWPHLAQHGTGQAISSYNTMFTGP
                     RKKNEPHGPSHMYVILLDNGRSNLYAQEKLNVALSCIRCGACLNACPIYKNVGGYTYN
                     TTYQGPIGTVISPHLGNFQEHIHLSTACSLCAGCTEVCPVKIPLHELIHENRKRAVSQ
                     NFFPLTEQLGMKAFEYVASKSKRMDLIGSTLKNILLSIAGSRLWGKKREFPKIAKQSF
                     RKQYLKLAKKQKNTKQ"
BASE COUNT          596 a          243 c          282 g          491 t
ORIGIN      
        1 atgaagtaat taaaatagtt actgtatttt tgaatactca atttgaggga ggacgtcacg
       61 aacgacgagt taataaaata ccacttttat aaatacatat ataatacaat gtcacattct
      121 tccaaaaaac tgtttaaaca tgctgccgaa cgaatagcgt ttgatgagca gcatagaaaa
      181 actatacagt ttaatatggg aaaatatcat gcagctgttg gtgccggaaa attacgatat
      241 gcaaatttag atgcagctcg tattaaagcc tcggagatta aacgacatac attagctaat
      301 ttgccacaat atttagaaga atttgagaaa aattgtattc aaaacggtat tacagttctt
      361 tgggctcaaa ccgctgccga tgcaatacaa tatataacaa atattttgca agaacacaag
      421 gtaacatcgg ttgttaaaag taaatctatg actaccgaag aaattgaact gaatcatgaa
      481 ttagaaaaaa tcggaataga atctgttgaa actgatttag gtgaatatat agttcaattg
      541 gcaggagaaa aaccatatca tatagtaact ccagctatgc ataaatcaaa acaagatgtt
      601 gctgtattgt ttcatcaaaa atttggattt ccgcttacaa gtaccgcaga agaaatgacg
      661 aatttagcac gtcagaaatt acgtgaaaaa tatttggcag caggtgcagg tattacagga
      721 gcaaattttt tgattgcaga taaaggtgga gtttgtgtta ccgaaaatga aggtaatgca
      781 atcatgtcaa cagcatttcc tgatatacat attgcaatag caggaattga aaaagtaatt
      841 ccttcattca aagatttagc tctgttttgg cctcatttgg cacaacatgg aacaggacaa
      901 gctatttctt catacaatac tatgtttacc ggtcctcgca aaaaaaatga gcctcacgga
      961 ccgtctcata tgtatgtaat tttactagat aatggtagaa gtaatctata tgcacaagaa
     1021 aaattgaatg ttgctctttc atgtatcaga tgcggagcct gtttaaatgc ttgtcctata
     1081 tataaaaatg ttggaggata tacttacaat acaacttatc agggacctat aggaacagtt
     1141 atatctccac atttaggtaa ttttcaagag catattcatt taagtacggc atgttctcta
     1201 tgtgcagggt gcaccgaagt ttgtcctgtt aaaatacctt tacatgaact aattcatgaa
     1261 aatagaaaac gagctgtaag tcaaaacttt ttcccactta cagagcaatt aggaatgaag
     1321 gcgtttgagt atgttgcttc taaatcaaaa cgaatggatt tgataggatc aacattaaaa
     1381 aatatacttc tttctattgc aggttcacgt ttgtggggta aaaaacgtga atttccaaaa
     1441 attgcaaaac aatcatttcg aaagcagtat ttgaaattgg ctaagaaaca aaaaaatacg
     1501 aaacagtaat ttataattag tatacttctg gaatagtaaa atatgattta cataattcgc
     1561 atataaatca tattttacaa agttttagtc tattatacta gtatatatta tt
//
LOCUS       DAOTOW010000589         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_60024_length_1609_cov_3.339125, whole genome shotgun sequence.
ACCESSION   DAOTOW010000589 DAOTOW010000000
VERSION     DAOTOW010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60024_length_1609_cov_3.339125"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="P5243_09515"
     CDS             complement(<1..1050)
                     /locus_tag="P5243_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRS19730.1"
                     /translation="MEKQSKIYIAGHLGLVGSAIWKILQNKGYTNLIGKSIQELDLMN
                     PQAVADFFAAEKPEYVILAAAKVGGIVANNTYRAQFIYENLMIQNNVIHNAYVHGVKK
                     LLFLGSTCIYPKEAPQPMSEDCLLTSPLEYTNEPYAIAKIAGIKMCESYNLQYGTNFI
                     SVMPTNLYGPNDNFNLETSHVLPALIRKIYLGKCIETNDWNAISKDLNKRPIEGISGK
                     NTQEEILAILEKYGIITPKPYTLNPIPKIEIWGTGAPMREFLWSEEMAEACVYCIENV
                     NFSDVRGSGKEVRNTHINIGTGKDISIRDLAYTIKQIIGYQGEIFFNADKPNGTMKKL
                     TNPSKIHSLGWHHSIE"
     gene            complement(1093..1455)
                     /locus_tag="P5243_09520"
     CDS             complement(1093..1455)
                     /locus_tag="P5243_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS19731.1"
                     /translation="MGDFTKLQVWILAKELAVNIYSLTASEKFSKDYDMRNQLRRAAV
                     SVPSNIAEGEESGSNPQSIRFFNIAKGSLAELRTQCIIAFEIGYLNSDDYTCIHDKAL
                     KISSMLHKLIQSRKTTKP"
BASE COUNT          453 a          291 c          275 g          590 t
ORIGIN      
        1 ttcaatagaa tgatgccaac ctaacgaatg tattttcgac ggattagtta attttttcat
       61 tgtcccatta ggtttatcgg cattaaagaa tatttcgcct tggtatccaa taatctgctt
      121 aatggtgtaa gctaaatctc taatagaaat atctttaccg gtgccaatgt ttatatgggt
      181 atttcgcact tctttgccgg aacctcgtac gtcagaaaaa ttcacgtttt ctatgcaata
      241 cacacatgct tcggccattt cttcgctcca caaaaattcg cgcattggag caccggttcc
      301 ccagatttca attttcggta tagggtttaa ggtatagggt ttaggggtta taattccata
      361 tttttctagt attgcaagaa tttcttcttg tgtgtttttg cccgaaatac cttcgatagg
      421 acgtttgttt agatctttag aaatagcatt ccaatcgttg gtctctatgc atttgcctaa
      481 atatattttg cgaataagtg caggcaatac atgcgatgtt tcaagattga aattatcgtt
      541 ggggccatac aagttggtag gcattaccga aataaagttg gtgccatatt gtaaattgta
      601 actttcacac attttaattc cggcaatttt tgcaattgca taaggttcat tggtatactc
      661 aagtggcgat gtaagtaagc aatcttctga cattggctgg ggggcttctt tcggatatat
      721 gcaagtgctt cctaaaaaca ataatttttt caccccgtgc acataggcat tgtgaatcac
      781 attgttttga atcatcaaat tttcgtatat aaactgagcc cgataggtat tgtttgcaac
      841 aataccgcct actttagcag cagcaagtat cacatactcc ggtttttcgg cggcaaaaaa
      901 atcagcaacc gcctgcgggt tcatcaaatc aagttcttga atgctttttc ctataagatt
      961 ggtatatcct ttattttgta ggattttcca tatagccgac cctactaatc caaggtgtcc
     1021 tgcgatgtat atttttgatt gtttttccat tttttaattt tggtataagg tttacggtat
     1081 aaggtatatg gtttagggtt tagtggtttt tcttgattgg attaatttat ggagcattga
     1141 agatattttt aaagctttgt catgtataca tgtataatca tcgctattta aatagcctat
     1201 ctcaaaagct attatacatt gagttcgcaa ttcagctaaa gatccttttg caatattaaa
     1261 aaaacgaatt gattgaggat tggaaccaga ttcttcacct tcagcaatat tagatggaac
     1321 acttactgca gctcttctta attgattacg catatcataa tcttttgaaa atttctcgct
     1381 tgcagttaat gaatatatat taactgctaa ttcttttgcc aatatccaaa cttgtaattt
     1441 tgtaaaatca cccatatttt ttctttaaac tatatcccta caccgtatac cgtacacccg
     1501 tattattcaa aataattcat agttctatag ccgccttcac gtaagtatgc atctttttgc
     1561 attagtttta tatcagattt catcatgtct tgtactaatc cttgcaggt
//
LOCUS       DAOTOW010000590         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_60060_length_1609_cov_1.565637, whole genome shotgun sequence.
ACCESSION   DAOTOW010000590 DAOTOW010000000
VERSION     DAOTOW010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60060_length_1609_cov_1.565637"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="P5243_09525"
     CDS             <1..797
                     /locus_tag="P5243_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS19732.1"
                     /translation="TGEIIGCFGLTEPNHGSDPGSMVTRLYDKGDHYVLHGAKMWITN
                     ANIADIAVVWAKDDNGIVRGVIVEKGMKGFSAPETHNKWSLRASATGELIFDEVVVPK
                     ENLLPNIQGLKGPMSCLNSARYGIAWGAVGAAMDCYDAALRYSLERHQFGKPIAAYQL
                     QQKKLAEALTEITKAQLLVWRLGVMKNEGKCKHTHISMAKRNNVYMALNVARELRQVL
                     GAMGITSDYPIMRHMMNLESVITYEGTHDIHLLITGHDITGIPAYR"
     gene            887..>1609
                     /locus_tag="P5243_09530"
     CDS             887..>1609
                     /locus_tag="P5243_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19733.1"
                     /translation="MNTIEKEVILDDSRVIMESLAQAGADTYIGYPITPSNAMYLYAS
                     QRFQYMLPAPDEISTLQYMSGLAAEGHLPVTATSFPGFALMLESINMAYMMELPMIII
                     LTQRLGPATGTATAGAQGDVALLRGMISGGHPIPVVCPSSIEDCWELSAKAVQMAVEL
                     RCPVVILSSKERANTNRNFDISKLKPIEPIKRTYYEGKDEYIPYKYNDSLVPEFLPVS
                     NNEHQVRITASTHDQKGIIQHTN"
BASE COUNT          516 a          315 c          320 g          458 t
ORIGIN      
        1 caacaggtga aattattggc tgttttggtt tgaccgaacc caaccacgga agcgatcccg
       61 gaagcatggt aactcgttta tatgacaaag gagatcatta tgtacttcat ggtgcaaaaa
      121 tgtggattac caatgcaaat atagctgata ttgctgtagt ttgggcaaaa gatgataatg
      181 gtattgttcg cggtgtgatt gtagaaaaag gaatgaaagg tttcagtgca cctgaaactc
      241 acaacaaatg gtcattacgt gcatcggcaa ccggcgaatt aatttttgac gaagtggttg
      301 ttccaaaaga aaacctatta ccaaacattc aaggtttaaa aggacccatg tcgtgtttaa
      361 atagtgcacg ttatggcata gcctggggtg ctgttggcgc tgctatggat tgctacgatg
      421 cagcattgcg ttactctctc gaacgccatc aatttggcaa acctattgct gcctaccaac
      481 tccaacaaaa gaaacttgcc gaagcattaa ccgaaataac caaggctcaa cttttagtat
      541 ggcgtctcgg agttatgaaa aatgaaggaa agtgcaaaca cactcatatt agtatggcaa
      601 aaagaaataa tgtttatatg gcattgaacg tagctcgtga acttcgtcaa gtattaggtg
      661 ctatgggtat taccagcgat tatccaatta tgcgtcatat gatgaacctc gaatctgtaa
      721 ttacctacga aggcactcac gacatccatt tactcattac cggacatgat ataacaggaa
      781 ttccggcata cagataaaat attcctaaaa atgtttaatt ttgcagctac cttgtataaa
      841 aatctattac atggtagctg ttttttttaa tttaaattaa ttgataatga ataccataga
      901 aaaagaagta attttagatg attccagagt aattatggaa tcattggcac aagcgggagc
      961 cgatacatat atcggttatc ccataactcc gtcaaatgca atgtatctat atgcaagtca
     1021 gcgttttcaa tacatgctac ctgcgccgga cgaaatttct actttgcaat acatgtcggg
     1081 attagcagca gaaggtcatt tacctgtaac cgctacctcc tttcccggat ttgctctaat
     1141 gcttgagtca ataaacatgg cttacatgat ggaattgccc atgataataa ttctaactca
     1201 acgcttaggt cctgctacgg gaacggcaac ggctggagct caaggtgatg ttgcactact
     1261 tagaggtatg atttccggtg gtcaccctat tccggttgtt tgtccttcaa gtattgaaga
     1321 ttgctgggaa ctatcagcaa aagctgtcca aatggctgtt gaacttagat gtccggttgt
     1381 gattctttca tcaaaagaaa gagctaacac caatcgcaat ttcgatattt cgaaactaaa
     1441 accaatagaa cccattaaac gaacatatta cgaagggaaa gacgagtata ttccatataa
     1501 atataatgat tcgcttgttc ctgaattttt acccgtttct aacaacgaac atcaagtccg
     1561 aataactgcc tctactcacg accaaaaagg aattattcag cacacgaat
//
LOCUS       DAOTOW010000591         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_60277_length_1605_cov_2.526452, whole genome shotgun sequence.
ACCESSION   DAOTOW010000591 DAOTOW010000000
VERSION     DAOTOW010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60277_length_1605_cov_2.526452"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1605
                     /locus_tag="P5243_09535"
     CDS             <1..>1605
                     /locus_tag="P5243_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19734.1"
                     /translation="SNCPGCSDKLGRFYYGVINVNTASCTGYPSCSAFLGAIGIHVIL
                     GRRLWLNSSFIGYGLQANDERIYQWFDLTNWYGFTMAQPVVANRPYLKNNSTDNVNFN
                     PVMSFEGSNLNMDLDDNTLYGYGADGGVGIFAVVIPKNNTLQSVFDYGLSTTKGFGLR
                     YDTENLSAYTPTAHGGFLNTSTHTLTTTPSLITQTSKWGANQILYQNGVQKVSNSIAS
                     MTKMESTEIAHSATATAGVSGPFTLGGRSDNPTLQDFEGKLAELIIYAVDVNDSTRKS
                     TESFLALKYGITLPYDYTDFDGNTIYTVDATYKNGIVGIGRQDLNHLHQRQSKSLASG
                     ENLMLCTGTPVAYDQRKVTTQVAADKSFFVCGHNGLPTNNGTRVYKATTRNFNQQVTL
                     SFKFTGIDAPYPTLTIDNVDTFTGSPVTVTYDSYDGNYLVYKYTFPNNATRYFKVGAV
                     TTIPREPGVGINTTAIDTSAQLHVYSTNKGVLIPNLTTVQMNGIASPPTGLLIYNTTQ
                     NRYMFNAGTPASPAWSVVGRFITNTS"
BASE COUNT          524 a          264 c          304 g          513 t
ORIGIN      
        1 gttctaactg tccgggatgt tccgataaat taggtagatt ttattatgga gtaatcaatg
       61 tgaatactgc aagctgtaca ggctacccct cgtgttctgc ctttttaggg gctattggga
      121 ttcatgtaat tttaggacgt aggctctggt taaattcatc atttattggg tatggattgc
      181 aggcaaatga tgaacgtatt taccaatggt ttgatttgac aaattggtat ggttttacaa
      241 tggcacaacc tgtagttgca aatcgtccgt atttaaaaaa taatagtact gacaatgtaa
      301 actttaatcc ggtaatgagt tttgaaggtt ctaatcttaa tatggattta gatgataata
      361 cattatatgg ctatggtgca gatggcggtg ttgggatatt tgctgttgtt atacctaaaa
      421 ataatacgtt acaaagtgtt tttgattatg gtctttcaac aacaaaagga tttggtttac
      481 gctatgatac agaaaatcta tcggcgtata ctccaacagc tcatggtgga tttctgaata
      541 catctactca tacccttact actacgccat cgctcattac tcaaacaagt aaatggggag
      601 caaatcaaat tttatatcaa aacggtgtac aaaaagtaag taattcaata gcttccatga
      661 ctaaaatgga aagcacagaa atagctcatt ccgctactgc tactgctgga gtttctggtc
      721 cttttacctt agggggacgt tctgataatc caaccttgca agattttgaa ggtaagttgg
      781 cagaattaat aatctatgct gttgatgtaa atgattcaac aagaaaatct acagaatcat
      841 tcttggcttt aaaatatggt attacactac cgtatgatta tactgatttt gatgggaata
      901 ctatatatac agttgatgca acatataaaa atggaattgt tggaattggt cgtcaagatt
      961 tgaatcattt acatcaaagg cagtcaaaaa gtcttgcaag tggcgaaaat ttaatgttat
     1021 gtacaggtac accggtggcg tatgatcaaa gaaaagttac aacacaagtt gctgccgata
     1081 aaagtttctt tgtgtgtggt cataatggat tgcctacaaa taatggtaca agagtgtata
     1141 aagcaactac acgaaacttc aatcaacaag ttaccctatc attcaaattt accggtattg
     1201 atgctccata tccaacatta acaatagaca atgttgatac atttaccggt tcacctgtta
     1261 ctgttaccta cgattcgtat gatggaaatt atttggtata taaatataca tttcccaata
     1321 atgcaacccg atattttaaa gttggagctg ttactactat accgcgagaa ccgggagttg
     1381 gaataaatac aacggcaatt gatacatctg ctcaattaca tgtatattct accaataaag
     1441 gtgtgttaat accaaatctt acaacagtcc aaatgaatgg tattgcaagt cctcctacgg
     1501 gattattaat atataatact actcaaaaca gatatatgtt taatgcggga acacctgcaa
     1561 gccctgcctg gtctgttgtt ggtagattta ttacaaatac aagtg
//
LOCUS       DAOTOW010000592         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_60600_length_1599_cov_4.420337, whole genome shotgun sequence.
ACCESSION   DAOTOW010000592 DAOTOW010000000
VERSION     DAOTOW010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60600_length_1599_cov_4.420337"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /gene="sufC"
                     /locus_tag="P5243_09540"
     CDS             complement(<1..685)
                     /gene="sufC"
                     /locus_tag="P5243_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRS19735.1"
                     /translation="MLHIQNLHAAIDGKEILKGIDLHVKPGEVHAIMGPNGSGKSTLS
                     SVLAGREIFEVTQGEITFMGKNLLELAPEERACEGIFMAFQYPVEIPGVSMTNFMKAA
                     IEAKHKYQGTEPPSPTEFLKKMKEKRAMVGLPDKLTGRSVNEGFSGGEKKKNDIFQMA
                     MLEPKLAILDETDSGLDIDALRIVADGVNQLKTPDNAYIVITHYQRLLDYIVPDVVHI
                     LFDGKIIKTG"
     gene            complement(704..1243)
                     /locus_tag="P5243_09545"
     CDS             complement(704..1243)
                     /locus_tag="P5243_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19736.1"
                     /translation="MKYIDTETYSKLVSHIEFNMNDMIDAVERNVYPLDYVMSVALSQ
                     RQPQARLSCWVITKLLYRNSSAICPYISQAIAFLPHTTHTGQTREILRWFTICKPTHD
                     NDLGTLLDFCLSVLPNNTLPVAIKVHAMTIIENIAKKEPDIIPEFAAILEDIYMYQSV
                     GGKNKIQKLLKKYESVLHN"
     gene            complement(1270..>1599)
                     /locus_tag="P5243_09550"
     CDS             complement(1270..>1599)
                     /locus_tag="P5243_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRS19737.1"
                     /translation="RGLVRMAKSADNARNFSQCDSLLIGSTCSANTFPYIDVQNNTAT
                     VEHEATTSKIADDQIFYCNQRGLSTEDAVSLIVNGFAKEVLSQLPMEFAIEAHKLIAI
                     SMEGSVG"
BASE COUNT          453 a          319 c          282 g          545 t
ORIGIN      
        1 ctccggtttt tatgattttt ccatcgaata atatatgcac tacatcgggt acaatataat
       61 caagtaatcg ttgatagtgc gtaattacga tgtatgcatt gtcgggggtt ttgagctgat
      121 ttaccccatc ggctactata cgcaaagcat caatgtcgag tccactatca gtttcgtcca
      181 atattgccaa ttttggttct agcatagcca tctgaaaaat atcatttttc tttttctcac
      241 ctcccgaaaa tccttcattt accgaacgcc cggtgagttt atcgggtaag ccaaccattg
      301 cccgtttttc tttcattttt ttaagaaatt cggtaggact tggcggttcg gtgccttgat
      361 atttgtgctt agcttctatg gctgctttca taaaattggt catactcaca cccggaattt
      421 ctaccggata ttgaaatgcc ataaatattc cttcgcatgc acgttcttcg ggagcaagtt
      481 caagcaaatt tttacccata aaggtaatct caccttgggt aacttcaaat atttcgcgac
      541 ctgccaatac gcttgataat gtgcttttcc cggagccatt tggtcccatt atagcatgaa
      601 cttcacctgg ttttacgtgt aaatctatac cttttagtat ttctttacca tctatggctg
      661 catgtaaatt ttgtatatgt aacatgtgtc tttatttttt aaatcaatta tgtaaaacgc
      721 tttcgtattt ttttagtaat ttttgtattt tattttttcc tcccacggac tgatacatat
      781 atatatcttc caatatagca gcaaattcgg gtataatatc gggttccttt tttgctatat
      841 tttctataat agtcatagca tgtactttaa tagctacggg caatgtattg ttgggtaaga
      901 ccgataaaca aaaatcgagt aacgtgccta aatcattgtc gtgagtaggt ttacatatag
      961 taaaccaacg caatatttcg cgggtttgcc cggtatgcgt ggtatgaggt aaaaaagcaa
     1021 tagcttgtga aatatatgga caaatagctg aactattgcg atacaataat tttgtaatta
     1081 cccaacacga aagccgtgct tgaggttgcc gttgcgacaa tgccaccgac attacataat
     1141 ccaacgggta cacattgcgt tcaacagcat ctatcatatc attcatatta aactctatat
     1201 gtgatactag tttgctatat gtttcagtat ctatatattt cattttaata tatcacaaat
     1261 aaacatatat taaccaacac taccttccat agaaatagca attaatttgt gtgcttctat
     1321 ggcaaattcc attggcaact ggctgagtac ttcttttgca aatccattaa caataagact
     1381 tacggcatct tcggtgctta gaccacgctg gttgcaataa aaaatctggt cgtcggcaat
     1441 tttcgaagtg gtagcctcat gttctactgt ggcagtattg ttttgcacat caatatacgg
     1501 aaacgtattg gctgaacaag tagaacctat aagcaacgag tcgcactgcg aaaaattacg
     1561 agcattgtcg gcagattttg ccatacgtac cagtccgcg
//
LOCUS       DAOTOW010000593         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_60727_length_1597_cov_2.591440, whole genome shotgun sequence.
ACCESSION   DAOTOW010000593 DAOTOW010000000
VERSION     DAOTOW010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60727_length_1597_cov_2.591440"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1234
                     /locus_tag="P5243_09555"
     CDS             140..1234
                     /locus_tag="P5243_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRS19738.1"
                     /translation="MKVADIINEIEHIAPLSLQESYDNSGLLIGNSSQNVDSALLTID
                     ITEDVIDEAIALGTNLIISHHPLIFNGIKRINGNNYIERCIIKAIQHSIAIYCVHTNI
                     DSVQNGVNKKICDIIGLQNTQILRKQTNSLSKIVTFVPNEDAEKVRLALCNAGAGTIG
                     NYDNCSFNSQGKGTFRALDKAQPYIGTIGTIHTEPEIKIEVICHNYLIKHIIQALLSS
                     HPYEEPAYDIIPLANENKEYGSGMIGTLENPVDCNTFLEYIKTKFSCKSIRYSKTKKQ
                     TISKVAVCGGSGSFLITDAIKQQADIFLTGDVKYHDFFLSENKIIIADVGHYESEQFT
                     KEIFYEIINKKFPKFALQFSKVNTNPINYV"
     gene            1238..>1597
                     /locus_tag="P5243_09560"
     CDS             1238..>1597
                     /locus_tag="P5243_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19739.1"
                     /translation="MTSKKAETVQVTVEEKLKALYQLQEADSKIDKIQRLRGELPLEV
                     KDLEDELAGLQTRIEKIQAEIIQRKEDITAKKNEIKEAQNHIKKYEQQQMDIRNNREF
                     EAIAKEIEFQKLEIELCE"
BASE COUNT          653 a          224 c          228 g          492 t
ORIGIN      
        1 atattatttt tttgctaatg tacatgaatt tgtttataca aattataatc ataaataaat
       61 ttttaacctc caaaaaacaa attttcgagt atcataatcc cattttttat actattttta
      121 catttaaaat tgtatgtgaa tgaaagttgc agatatcatt aacgaaattg aacacattgc
      181 tccactctca ttacaagaat cttatgacaa tagtggttta ttaattggta attcttctca
      241 aaatgtagat tcagcattgc ttactattga tataacagaa gacgtcatag atgaagcaat
      301 agcattaggt accaacctca taatttctca tcatcctctt atttttaatg gcattaaaag
      361 gattaatggt aacaattata tagaacgatg cataataaaa gcaatacaac attctatcgc
      421 tatttattgt gttcatacaa acatagattc ggttcaaaat ggagtaaata aaaaaatatg
      481 cgatattata ggattacaaa atactcaaat acttcgcaaa caaacaaatt ctctttctaa
      541 aatcgtaaca tttgtaccca atgaagatgc agaaaaagtt cgcttagcac tttgcaatgc
      601 aggagcagga acaattggta attatgataa ttgttcattt aatagtcaag ggaaaggtac
      661 ttttagagca ttagataaag ctcaaccata tataggtaca attggcacca tacatacaga
      721 gcctgaaatt aaaatagaag ttatctgtca caactatctc ataaaacata ttatacaagc
      781 actactttca tcacatcctt atgaagaacc tgcgtatgat ataatacctc ttgcaaatga
      841 aaataaagaa tatggttccg gcatgatagg aacacttgaa aatccggttg attgtaatac
      901 ttttttagaa tatatcaaaa ctaaattttc ttgtaagagt attagatact ctaagaccaa
      961 aaaacaaaca atttcaaaag tagcagtatg tggaggttcc ggaagttttt taattacaga
     1021 tgcaattaaa caacaagccg acattttttt aacaggtgat gtaaaatatc atgatttctt
     1081 tctgtctgaa aataagataa ttatagcaga tgttggtcat tatgaatcag aacaattcac
     1141 aaaagaaata ttttatgaaa ttataaacaa aaaatttcct aaatttgccc ttcaattttc
     1201 aaaggttaat acgaacccga taaattatgt ataatatatg acaagtaaaa aagcagaaac
     1261 tgttcaagtt acggttgaag aaaagttaaa agctttgtat caattgcaag aagctgactc
     1321 taaaattgat aaaattcaaa gattacgtgg tgaattacca ttagaagtaa aagatttaga
     1381 agatgaatta gcaggcttac aaacacgtat tgaaaaaatt caagctgaaa ttattcaacg
     1441 taaagaagat attacagcta agaaaaatga gattaaagag gctcaaaatc acataaaaaa
     1501 atatgaacaa caacaaatgg acattcgaaa caatcgtgaa tttgaagcaa tagctaaaga
     1561 aattgaattt caaaaattag aaatagaatt gtgtgaa
//
LOCUS       DAOTOW010000594         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61007_length_1592_cov_2.834743, whole genome shotgun sequence.
ACCESSION   DAOTOW010000594 DAOTOW010000000
VERSION     DAOTOW010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61007_length_1592_cov_2.834743"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="P5243_09565"
     CDS             <1..186
                     /locus_tag="P5243_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19740.1"
                     /translation="FLAMCTHTDYMHHTKAFMDAVNRATNMNKYRFCELMDSGKFSFQ
                     LLENPEFKKEYCTMCNK"
     gene            331..>1592
                     /locus_tag="P5243_09570"
     CDS             331..>1592
                     /locus_tag="P5243_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19741.1"
                     /translation="MNRVLLVFVILIVSFHMGYSQEMYYKLYADAEYDKLEKRLVQAI
                     ANQPQNVLFNHVYGILYATKEYQKNDLNKAYEYLLQAKTLYPSISTSDKERFAKLGLT
                     QSRINKDFEDVCKQLWNIAVSDNTIEACNTFINKYEKAPKEIKEQALQYRNELAFAEA
                     KKTHTIQSYQLFIQTYPKAEQVQLAITLRDEIAFEQAMKTNTLDALREFMNNYPQSKF
                     YAQVNTLYSERIFSEATKNKDYKSYERFIRDNPKNPMLPIAIERMVEIAKEKQDIKML
                     KKTVDYSVGIHFNYALYEYFKEFSKDGEYLTLYTFVQQYPRTFLDTLLAKEFKIAEKG
                     ERLDLSKPYDTVMAKYFEDYIRLAAPKEKAFLALQKYISNDIVAKDWNTAIAKVKKFE
                     PYFGKNNKKISDLLQILSAQYDNNIIVRP"
BASE COUNT          613 a          207 c          255 g          517 t
ORIGIN      
        1 ttcttagcta tgtgtactca taccgattat atgcatcata caaaagcatt tatggatgca
       61 gtaaatcgtg ctaccaatat gaataaatat cgcttttgtg aactcatgga ttccggtaaa
      121 ttttcttttc aacttttaga aaatcccgaa tttaaaaaag aatattgtac tatgtgcaat
      181 aaatagggaa tacctttggg gtatagaata aaaataattt tctttattgc agcaaactat
      241 tcaaaatatt gtgccgtaaa caataaattg agtagttttg aattttagct aatattaatt
      301 attttgtact actttaaata gtgtatgggt atgaatcggg ttttgttggt attcgtgatt
      361 cttattgtga gttttcatat ggggtattca caagaaatgt attataaatt atatgctgat
      421 gccgaatatg ataaattaga gaaacggttg gtgcaagcta ttgcaaatca acctcaaaat
      481 gtattgttta atcatgttta cggtatatta tatgcaacca aagaatatca aaaaaatgat
      541 ttgaacaaag catatgaata tttgttgcag gcaaaaacac tatatccatc tatttcaaca
      601 agtgataaag aacggtttgc aaaattaggc cttactcaat caagaattaa taaagatttt
      661 gaagatgtgt gtaaacagtt gtggaatatt gcggtttctg ataatactat tgaggcttgc
      721 aatactttca taaataaata tgaaaaggca cccaaggaaa ttaaagaaca agctctgcaa
      781 tatagaaatg aattagcatt tgccgaagca aaaaaaactc atactataca atcatatcaa
      841 ttatttattc aaacgtatcc taaagcagag caagttcaac ttgctattac gcttcgtgac
      901 gaaattgcgt ttgaacaagc aatgaaaact aataccttag atgcattgag ggaatttatg
      961 aataattatc cgcaaagtaa attctatgca caagttaata cattgtattc tgaacgaatt
     1021 ttttctgaag caacaaaaaa taaggattat aaaagttatg aacggtttat aagagataat
     1081 cctaaaaatc ctatgttgcc aatagctata gagcgaatgg ttgaaatagc aaaggaaaag
     1141 caagatataa aaatgctaaa aaaaacagtt gattatagtg tcggtattca ttttaactat
     1201 gctctttatg aatattttaa agaattttca aaagatggtg aatatcttac tttatataca
     1261 tttgttcaac aatatccacg aacattttta gatacccttt tagctaaaga gtttaaaatt
     1321 gccgaaaaag gtgaacggct tgatttatca aaaccatatg atactgtaat ggcaaaatat
     1381 tttgaagatt atattcgatt agctgcacct aaggaaaaag cgtttttggc tttgcaaaaa
     1441 tatatttcaa acgatatagt tgcaaaagat tggaatacag ctattgcaaa agttaaaaaa
     1501 tttgaaccgt attttgggaa aaacaataaa aaaatatcag atttattgca aatattatct
     1561 gcacaatatg acaataatat aattgtaaga cc
//
LOCUS       DAOTOW010000595         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61070_length_1591_cov_3.272786, whole genome shotgun sequence.
ACCESSION   DAOTOW010000595 DAOTOW010000000
VERSION     DAOTOW010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61070_length_1591_cov_3.272786"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="P5243_09575"
     CDS             complement(<1..515)
                     /locus_tag="P5243_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRS19742.1"
                     /translation="MGINNEKEYASLDDLQLDLERKSGDGGFFLTTLSDLVNWGRANS
                     LWPLTFATSCCGIEMMAAGAPRHDFSRFGMEVYRASPRQADVIVVAGTIVHKMAPVLK
                     RLYDQMSEPKYVIAMGACAVSGGPFFYNSYSVIRGADQVIPVDVYIPGCPPRPEAFFH
                     GVLELHKKIKQ"
     gene            complement(506..856)
                     /locus_tag="P5243_09580"
     CDS             complement(506..856)
                     /locus_tag="P5243_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRS19743.1"
                     /translation="MNTEGLILFFLLGVTLVGLAVIFSGLIPQKTTNLQKHDTYECGI
                     PTQGSSWIQFNVGYYLFAIMFLIFDVEAIFLFPWAVVMKEIGMVAFIEILIFFVILGL
                     GLLYAWKKQALKWE"
     gene            complement(1428..1577)
                     /gene="xseB"
                     /locus_tag="P5243_09585"
     CDS             complement(1428..1577)
                     /gene="xseB"
                     /locus_tag="P5243_09585"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRS19744.1"
                     /translation="MIKEIEQGEISIDTLSQQVKKAMELIHLCKNKLQTTEAELQKLL
                     QTDQD"
BASE COUNT          480 a          290 c          261 g          560 t
ORIGIN      
        1 tcttgtttga tttttttgtg taattcaagc actccatgaa aaaatgcttc gggacgtgga
       61 ggacagccgg gaatgtaaac atctaccgga attacttggt cggcaccacg aattaccgaa
      121 tatgaattgt aaaaaaacgg acctcccgat acggcacatg ctcccatagc aattacatac
      181 ttaggttccg acatttgatc gtataagcgt ttgagtaccg gagccatttt gtgtactata
      241 gttcctgcta ccactattac atcggcttgg cgaggcgaag cacgatatac ttccatacca
      301 aatcgtgaaa agtcgtgacg aggagctccg gcagccatca tttctatacc gcaacagctg
      361 gtagcaaagg taagaggcca caatgaattt gctctacccc aatttactaa atcacttaat
      421 gtagttaaga aaaatccccc gtctcccgat tttctctcta aatctaattg taaatcatca
      481 agcgatgcgt attctttttc gttatttatt cccattttaa tgcttgtttt ttccatgcgt
      541 acaaaagacc caaacctaat attacaaaaa atatgagtat ttcaataaat gctaccatac
      601 ctatttcttt cattaccact gcccatggaa acaagaaaat agcctccacg tcgaaaatca
      661 gaaacataat ggcaaacaaa taatacccta cattgaactg tatccacgac gaaccttgcg
      721 taggaatacc acattcatat gtatcatgtt tttgtaaatt tgttgtcttt tgaggaatta
      781 agccggaaaa aattaccgct agcccaacga gtgtaacccc taggaggaaa aataaaatca
      841 aaccctcagt attcatgaga aaatgttttt gtatgttttt ttttatgttt tttatcgtgc
      901 aaatatatgt gatatttaca tatacacaaa ttatttaaaa aaagatttta atattttttt
      961 gaacgaatgt aaaatgctgt tttttaaaac cttatatatt ttattactaa atttatatgt
     1021 tacaatttta aaaatattac tacttcgggg ggggtaagtt gtgataattt atttggatct
     1081 ataataatgt cacccctccg gggttcattt agttatgcta taatggttgt tctataataa
     1141 ttacacccct tcggggtttt tatgtggtgt ttgattggtt tcatttataa taatgacatt
     1201 cctacgggat tattatattt ttaacatttc tctataataa tatcacccct tcggggtttt
     1261 aattttttga tgatattttt taataattat atgtctccgg aatcgggtat aataatatta
     1321 tgtattggtt ggttgattga agcccgaggg gctgacatta ttatagaaac ccgttgtaaa
     1381 cacacacgta gaaccccgaa ggggtgatat aacattacaa atcataatta atcttggtct
     1441 gtctgcaaca atttttgcaa ttcggcctcg gtggtttgca atttattttt acatagatgg
     1501 attaattcca ttgctttttt tacttgttgg ctcagagtgt ctatactaat ttctccctgc
     1561 tctatttctt ttattatttt ttctacttct g
//
LOCUS       DAOTOW010000596         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61309_length_1587_cov_2.928851, whole genome shotgun sequence.
ACCESSION   DAOTOW010000596 DAOTOW010000000
VERSION     DAOTOW010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61309_length_1587_cov_2.928851"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>1587
                     /locus_tag="P5243_09590"
     CDS             171..>1587
                     /locus_tag="P5243_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRS19745.1"
                     /translation="MKFIISTIGFLFCLLYSTHVIQAQSILKEGTWVKVALTESGVYK
                     ITYDELLQMGLPKAPSVHIYGNGAKELPLINSNELPSVLNEIAIWIEKGSDNIFNSGD
                     YILFYGEATSGWIYDTVQSMYTYMSHPYCSMNYYYITTNSTAPKIIQTSSQVTQPHTK
                     TTSTFDDLYIYEQNTVNPLKSGRMWFESISNKTVTFSIPNIAIQEPTYIHTQIAGRHY
                     DTGKFFIKINNQAFDTISVWNTSNNNPYAVLQSSRKQYTASSTNLSIQISTNISGVDS
                     RAFLDFCSIHARSRLSFNNSQLLFRDIQSVAPNSCTQFEINSNTQATIWDISSPANPQ
                     KIITHFSNNTITFKALSSQLHEYIAFSNQFKSVTFVSTVQNQDILSDTDFDMIIIYNN
                     PSFIPYAQQIASVHETYDGLRTRIVNQQDIFTEFSAGRIDISAVRNYVRWVYTKNNGK
                     LQYVLLFGDGTYNNSLCNNNSN"
BASE COUNT          580 a          282 c          208 g          517 t
ORIGIN      
        1 agaaaaatcg ggtgtttggg cctgaacaaa cgacacaatt aagagcaata gtatctcaaa
       61 aaaaagatat tttaggcttt tagttatcaa gttttactat ttttataccg tcaaaaatac
      121 tatttcaatt tatatttacg tattatagaa ttatatattt taaaaatcac atgaaattta
      181 ttatttcaac tattgggttt ttgttttgtt tactttattc tactcatgta attcaagcac
      241 aatcaatttt aaaagaaggc acatgggtaa aagtagcatt aacagaatcc ggcgtttata
      301 aaattactta tgatgaattg ttacaaatgg ggttacctaa agcaccatcg gtacatatat
      361 atggcaatgg tgccaaagaa ttaccactca tcaattccaa tgaattacca agcgtactca
      421 atgaaattgc catttggatt gaaaaaggtt cagataacat atttaattcc ggtgattata
      481 tcctgtttta cggagaagct acaagtggtt ggatatacga taccgtacaa tctatgtata
      541 catacatgtc gcacccatat tgttctatga attactatta tattacaacc aattctacag
      601 ctcccaaaat aatacaaaca agttcgcaag taacacaacc ccacaccaaa acaacctcta
      661 catttgatga tttatatata tatgaacaaa acactgtaaa tccacttaaa tcaggcagaa
      721 tgtggtttga aagcatttct aataaaaccg ttacattttc tattcccaat attgcaatac
      781 aagaaccaac atatattcat actcaaattg ccggacgaca ttacgatacc ggcaaatttt
      841 ttataaaaat aaataatcaa gcctttgata ctataagtgt atggaataca tcaaataata
      901 acccatatgc ggttttacaa tcaagcagaa aacaatatac agccagctct actaatctgt
      961 ctatacaaat ttcaacaaat atttccgggg ttgacagtcg agcattttta gatttttgca
     1021 gtattcatgc tcgtagtcgt ctttcattca acaattctca attactcttt cgtgatatac
     1081 aatcagttgc tcccaatagc tgtacacaat ttgaaataaa ttcaaacaca caagctacaa
     1141 tttgggatat ttcatcacct gcaaatcctc aaaaaatcat cacacatttt tcaaacaata
     1201 ctattacatt taaagcatta tcatctcaat tgcatgaata cattgctttc tcaaatcaat
     1261 ttaaatcagt aacttttgtt agtactgtcc aaaatcaaga tatactgtcc gatactgatt
     1321 ttgacatgat aataatttat aataatcctt cttttatacc atatgcacaa caaatagctt
     1381 cggttcatga aacatatgac ggattacgca ctcgtattgt taatcaacaa gatattttta
     1441 cagaattttc tgccggaaga atagacataa gtgctgttcg taattacgtg cgatgggttt
     1501 atacaaaaaa caacggcaaa ttacaatatg tattactctt tggtgacggt acatataata
     1561 atagtttatg taataataac agcaatt
//
LOCUS       DAOTOW010000597         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61362_length_1586_cov_2.767472, whole genome shotgun sequence.
ACCESSION   DAOTOW010000597 DAOTOW010000000
VERSION     DAOTOW010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61362_length_1586_cov_2.767472"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="P5243_09595"
     CDS             <1..525
                     /locus_tag="P5243_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS19746.1"
                     /translation="QLDQLKSGMIEDGTAIGTGLGLSVTRIKDSKAISKVVVLLTDGV
                     NNRGDIDPLTAAELAKKYGVRIYTIGVGTQGEAPYPVQTLWGIEFQMMPVEIDEVVLQ
                     KIANITGGKYFRATNKKELLEIYQTIDSLEKTKIEDYKFTTKKEMYAGFVLWALVFLV
                     LEFIFKQFGLRKYL"
     gene            533..>1586
                     /locus_tag="P5243_09600"
     CDS             533..>1586
                     /locus_tag="P5243_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS19747.1"
                     /translation="MNETNFRFANPEYLYALTGVFIFVFLFVWYRYVRTKKIANLADS
                     HLIDYVIPLQSPIRITIKFILFSLAYICIIIALARPQFGITIDSVKKRGIELVIALDV
                     SNSMLAQDVKPSRLERAKLSIQNIINKLQDDKIAIVVFAGDAYIQLPLTTDIHAAKLF
                     LQSISTASIQKQGTAIGAALAKSVRSFTDNPKTGKAIIVISDGENHEDDAQAEARKAV
                     EKGIVIHTIGMGSPQGAIIPRSAGGFVEDANGRPVTTKLDEEMLQKIASIGNGIYSRE
                     NFSPVIHSIESMTKTEFETKQYSQYDEKFPLFLLAAFVCLFLELIILERKNKWLQQFD
                     IFTILSTNVSEGAIYTN"
BASE COUNT          566 a          207 c          294 g          519 t
ORIGIN      
        1 caattagacc aattgaaatc gggaatgatt gaagatggca cagcaatagg taccggatta
       61 ggtttaagtg ttacacgaat aaaagatagt aaagctataa gtaaggttgt agtattgctt
      121 acagatggtg taaataatag gggagatatt gatccgctta ctgctgcaga attagctaag
      181 aaatatggcg ttcgtatata tactattggg gtcgggactc aaggtgaagc tccatacccg
      241 gtgcaaacat tatggggaat agagtttcaa atgatgcccg tagaaattga tgaagttgta
      301 ttgcaaaaaa ttgccaatat tactggtgga aaatatttta gagcaactaa taaaaaggag
      361 ttgttagaaa tttatcaaac aattgattct ttagaaaaaa ctaaaattga agattataag
      421 tttactacca aaaaagaaat gtatgcaggt tttgtattgt gggctttagt atttttggtt
      481 ttagaattta tttttaaaca atttggatta agaaaatatt tgtaattgca gtatgaacga
      541 aacaaatttt agatttgcta atcctgaata tttatatgct cttacagggg tatttatatt
      601 tgttttttta tttgtttggt atcgttatgt gagaacaaaa aaaatagcaa atcttgcaga
      661 ttctcattta attgattatg taattccttt acaatcacct attcgtatta caattaaatt
      721 tattcttttt tcacttgcgt atatatgtat tattatagca ttggcacgtc ctcaatttgg
      781 aattaccatt gatagtgtaa aaaaacgagg catagaattg gttatagcac ttgatgtttc
      841 taatagcatg cttgcacaag atgtaaaacc ctctcgttta gaaagagcca aactttctat
      901 tcaaaatatt ataaataaac tgcaagacga taaaattgca attgttgtat ttgccggtga
      961 tgcatatata cagttgccat taacaacaga tatacatgcc gctaaattat ttttgcaatc
     1021 aatatcaaca gcaagtattc aaaaacaagg cactgctata ggtgccgcat tagctaaaag
     1081 tgttcgttcg tttactgata atcctaaaac cggtaaagct ataatagtaa taagtgatgg
     1141 agaaaaccat gaagatgatg ctcaagccga agctcgtaaa gctgtagaaa aaggaattgt
     1201 aattcatact attggaatgg ggagtccaca aggtgctatc ataccacgtt ctgccggagg
     1261 atttgtagaa gatgctaatg gacgcccggt tactaccaaa ttagatgaag aaatgttaca
     1321 gaaaatagca agtataggta atggtatata ttcacgtgaa aatttttctc ctgttataca
     1381 ttctatcgag agtatgacaa aaacagaatt tgaaacaaaa caatatagtc aatatgatga
     1441 aaaatttcct ttgtttttgt tagcagcatt tgtatgtttg tttctggaat taattatatt
     1501 ggaacgaaaa aataagtggt tacaacaatt tgatatattt acaatattat ctactaatgt
     1561 atcagaaggg gcaatttata ctaata
//
LOCUS       DAOTOW010000598         1583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61527_length_1583_cov_2.407068, whole genome shotgun sequence.
ACCESSION   DAOTOW010000598 DAOTOW010000000
VERSION     DAOTOW010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61527_length_1583_cov_2.407068"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1292
                     /locus_tag="P5243_09605"
     CDS             168..1292
                     /locus_tag="P5243_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRS19748.1"
                     /translation="MDNISNSFTLQQKKIVVCNYAHYCFVISPQTQNSFIEQMQAIIE
                     GITYLYSQYNFTKTNARFARIFLSDIQNQYDYIVQSELFDLFLYAQSTSIIQQIPCNN
                     SKITLLLTCTDYSNSIIHTNNDSIGFVENEYTFWWTTNRMYTTENSVQKQTIEILNSY
                     QKELKTIDCSIQESCVRTWFFIRDIDTRYAAMVQARKEMFQNIDLTPETHFITSTGIE
                     GAQYSPQSLVSFDALAIQGLHLSQIQYLLAPTHLNPTHEYGVTFERGVAMLFSNYNLI
                     LISGTASINNKGEIVAVGDMKAQLDRTFENIKALFLECNAKISDILYSIVYIRDIHDT
                     ALVHNYMKKNYSDMVYVLVQAPVCRPGWLVEIECVAYSVK"
     gene            1366..>1583
                     /locus_tag="P5243_09610"
     CDS             1366..>1583
                     /locus_tag="P5243_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRS19749.1"
                     /translation="MKLNTKTRYGLRAIIEIAMNSSEHGVLQKDIAKTQNISNKYLDQ
                     IIAELKSADLIVNTGGKKSGYRLNGDPK"
BASE COUNT          572 a          233 c          252 g          526 t
ORIGIN      
        1 gctcgtaata aatatttttt aaaaaattat aaaggagcaa acgacgatat aacaaaagcc
       61 ttagatttga atcaaaaaat gttaaatgcc gatccgtatg ctcgcagcag aagaaatgaa
      121 atggaagcat ttgctcaaaa aattgcaaaa gaattaaaaa ccttgtaatg gataatataa
      181 gtaattcatt tacattacaa caaaaaaaaa tagtagtgtg caattatgca cactattgtt
      241 ttgtaatatc tccacaaaca caaaattctt ttattgagca aatgcaggca ataattgaag
      301 gtattacgta tttgtatagt caatataatt ttactaaaac aaatgctcgt tttgcccgta
      361 tatttttgag cgatattcaa aatcaatatg attatattgt acaaagtgaa ttgtttgatt
      421 tgtttttgta tgctcaatct acttctatta ttcagcaaat tccttgtaat aactctaaaa
      481 ttacattatt gcttacctgt actgattatt ctaattctat tattcatact aataatgatt
      541 ctattggatt tgttgaaaat gaatatacgt tttggtggac aacaaatcgt atgtatacta
      601 ccgaaaattc tgtacaaaaa cagacaattg aaatattaaa ctcgtatcaa aaagaattaa
      661 aaacgattga ttgttcaata caagaatctt gtgtgcgtac gtggtttttt attcgcgata
      721 ttgatacccg ctatgctgct atggtgcaag ctcgcaaaga gatgtttcaa aacattgacc
      781 ttacacccga aacacatttt attacaagta ccggtattga gggggcacag tattctcctc
      841 aatcattagt gagttttgat gcacttgcta ttcaagggtt acatttatcg caaattcaat
      901 atttgttagc tccaacacac cttaatccta cacatgagta tggagtaact tttgagcgcg
      961 gtgttgcaat gttattttct aactataatc ttattttaat aagtggaact gccagcatta
     1021 ataacaaagg cgaaattgtt gctgttggcg atatgaaagc acaattagat agaacgttcg
     1081 aaaatattaa agcgttattt ttagaatgta atgcaaaaat ctccgatatt ttatacagta
     1141 tagtatatat acgcgatatt catgataccg ctcttgtaca taactatatg aaaaaaaatt
     1201 attctgatat ggtatatgta cttgtgcaag cgcccgtttg tcgacccggt tggttagttg
     1261 agatagaatg tgttgcatat tctgtaaaat aatttcaaat actatttgac ttttatattt
     1321 tatgtagtat atttgtatcc tactaatccg ataggataat ataatatgaa attaaataca
     1381 aaaactcgat acggactacg tgctattata gaaatagcta tgaatagcag tgagcatggt
     1441 gtgttgcaaa aagatattgc aaaaacacaa aatatttcca ataaatattt agatcaaatt
     1501 attgcagaac tgaaatctgc cgatttaata gtaaataccg gaggtaaaaa aagcggttac
     1561 cgactcaatg gtgaccctaa aaa
//
LOCUS       DAOTOW010000599         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61596_length_1582_cov_2.294041, whole genome shotgun sequence.
ACCESSION   DAOTOW010000599 DAOTOW010000000
VERSION     DAOTOW010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61596_length_1582_cov_2.294041"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="recF"
                     /locus_tag="P5243_09615"
     CDS             complement(<1..716)
                     /gene="recF"
                     /locus_tag="P5243_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRS19750.1"
                     /translation="MYLSKLHTFNYRNIEDLSLDASIGIHAIIGKNGVGKTNILDSIY
                     YLAFCKSYIANSDVQNVMIGANSFAIQGIFEHNASHFKIHCGYKIDTKEKTILCNDTK
                     YTKFSDHIGLIPIVFVSPADMVLINGSGSERRKFIDAYISLYNKEYLNHLLAYNKLLA
                     QRNVILKQGSVDATYIAIIDEKMAEHMNAIFTIRNQSIHELSSLLASLYHQISSFEDC
                     KLQYQSQLQQIDAFNGLHKS"
     gene            959..>1582
                     /locus_tag="P5243_09620"
     CDS             959..>1582
                     /locus_tag="P5243_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRS19751.1"
                     /translation="MSKNVGNIVQIIGPVVDVSFEQEGSTLPKILDALEITKENGQVV
                     VLECQQHIGEHTVRTVAMDSTDGMSRGMKVVATGAQITMPVGDAVRGRLFNVTGDAID
                     GIGEVSKANGYQIHNAPPAYEKLSTSSEILYTGIKVIDLIEPYAKGGKIGLFGGAGVG
                     KTVLIQELINNIAKAYEGLSVFAGVGERTREGNDLLREMIESGIVKYG"
BASE COUNT          606 a          215 c          254 g          507 t
ORIGIN      
        1 atacttttgt gtaagccatt aaaagcatca atttgttgta attgtgattg atattgaagt
       61 ttacaatctt caaaacttga aatttgatga tataatgaag ctaataacga tgataattca
      121 tgaattgatt ggtttctgat tgtaaaaata gcattcatat gttcagccat tttttcatct
      181 attatagcaa tatatgttgc atctacagaa ccttgtttga gaattacatt tcgttgagca
      241 agtaatttat tatatgcaag taaatgattc aaatactctt tattatataa tgatatatat
      301 gcatcaataa atttacgtcg ttcacttcca ctaccattta taagaaccat atctgcaggc
      361 gaaacaaaaa ctataggtat taaaccaata tggtcagaaa attttgtgta ttttgtatca
      421 ttacacagta tagttttttc tttagtgtca attttgtaac cacaatgaat tttaaaatga
      481 gaagcattat gttcaaaaat tccttgtatt gcaaaagaat tagctccaat cataacattt
      541 tgcacatcag aatttgcaat atagctttta caaaatgcta aataatatat actatcaaga
      601 atattagttt taccaacccc atttttgcct ataattgcat gaattcctat agatgcatct
      661 aatgacaaat cttcaatatt tctataatta aatgtgtgta attttgataa atacatacta
      721 tttttttgtg caaaatacga aaaaaagaat ttacagaaca actatataca taaattcaaa
      781 aacatatata atttacttat gtaaataatg aatgtataag aataaaaagt aaacaataat
      841 attggcaaaa gaaaatcata tcattttttt caaaaaaaaa ctaagaatcc tttgtaaata
      901 tatagaaaat actaattttg taataactaa gaaaagaata atctaaaatt acgattttat
      961 gtctaaaaac gtaggtaaca tagttcaaat tattggtcct gtagttgacg taagttttga
     1021 acaagaaggc agcacactgc caaaaattct tgatgcatta gaaataacta aagaaaatgg
     1081 tcaagtagta gtattagaat gtcagcaaca tattggcgag catacagtac gaaccgttgc
     1141 aatggattca accgatggaa tgagccgagg gatgaaagta gttgctaccg gagcacaaat
     1201 tactatgcct gtaggtgatg ccgttcgcgg acgtttattt aatgtaacag gagatgccat
     1261 agatggaatt ggagaagttt caaaagcaaa tggatatcaa atacacaatg ctccacctgc
     1321 atacgaaaaa ttatcaacaa gttcggaaat actgtacacc ggtattaaag taatagacct
     1381 gatagaacca tatgcaaaag gtggaaaaat tggtctgttt ggtggtgcag gagttggaaa
     1441 aacagtactt attcaagaat taatcaacaa tattgcaaaa gcatacgaag gcttatcggt
     1501 atttgccgga gttggagaac gtactcgcga aggaaatgac ttattgcgag aaatgataga
     1561 atcaggtatt gttaaatatg gt
//
LOCUS       DAOTOW010000600         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61760_length_1579_cov_2.329396, whole genome shotgun sequence.
ACCESSION   DAOTOW010000600 DAOTOW010000000
VERSION     DAOTOW010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61760_length_1579_cov_2.329396"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1493)
                     /locus_tag="P5243_09625"
     CDS             complement(24..1493)
                     /locus_tag="P5243_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19752.1"
                     /translation="MKPFVSIVLLLIWNYAFSQFQTISSGNWNNPSTWLGNNIPNSPN
                     DDVEICNGYTITLNTNVSVRNITITQGSLILGNNRIAIYGNVLGPNKNNFVSNSNTEL
                     QVLGGYDVPQFIMPLQVQKLKKLVINRKQGIFSNHSIDLDDGVPADGIVLVLQLGILY
                     MENESIVYLNSKQIQSNIPCSDSSHIDGIVQRNIPKNAGIYTFPVGNKGFCRPFGVGL
                     QNGNSDNINQVRFLYETPIQNTNINYNNLPGGIIQYFYWKHTVISGANPQRRLQYSYD
                     DFPGISSQERIQSMTLANTNGTSAWDKATTPWSVYDDSLWVQFNNANASNNEYWTLGS
                     INSEVKYENIKLPIELVDFWSRLITNNKIELHWTTAQEINNSYFVIFRSINGTDFMPI
                     DTVEAKGNSRDFTYYEYLDYIEQSADSILYYKLKQVDMNGSSSESHIIEVFIPYTQTV
                     AENIFECKPLPGYIELHIDIQQNPITNIIISCQVSQGCL"
BASE COUNT          547 a          249 c          205 g          578 t
ORIGIN      
        1 gttttatctt tgcaattaag ttatcataaa caaccttgtg aaacttgaca actaataatt
       61 atatttgtta ttggattttg ttgtatatca atatgtaatt caatataccc cggtaaaggt
      121 ttacactcaa aaatattttc agcaactgtt tgtgtataag gaataaaaac ttcaataata
      181 tgagattcac tactactacc attcatatca acttgcttaa gtttataata caatattgaa
      241 tcagctgatt gttctatata atccaaatat tcataatacg taaaatctct actatttcct
      301 tttgcttcaa cagtatctat tggcataaaa tcagtaccat ttatagagcg aaaaatcaca
      361 aaatatgagt tattaatttc ttgtgctgta gtccaatgga gttcaatttt attatttgta
      421 atcaaccgag accaaaaatc aaccaattct ataggtaatt taatattttc atatttaact
      481 tcagaattta ttgaaccaag agtccaatat tcattattag atgcattagc attattaaat
      541 tgcacccaaa gagaatcatc gtaaaccgac caaggtgtag ttgctttatc ccaagctgac
      601 gtgccatttg tattggctag agtcattgat tgaattcgtt cttgcgatga tattcccgga
      661 aaatcatcat aactatattg caatctgcgt tgaggatttg ctccacttat tacagtatgt
      721 ttccaataaa aatattgaat tatccccccc ggcaaattat tgtaattaat attggtattc
      781 tgtataggag tttcatataa aaaacgaact tgattaatat tatcagaatt accattttgt
      841 aaaccaaccc caaatggtct gcaaaatcct ttattcccaa caggaaaagt ataaatacct
      901 gcatttttag gtatatttct ttgtacaatt ccatcaatat gcgacgaatc ggaacatgga
      961 atatttgatt gaatttgttt actatttaaa tatacaatac tttcattttc catatatagt
     1021 atacccaatt gtaaaactag tacaattcca tcggcaggaa cgccatcatc taaatcaata
     1081 ctgtgatttg aaaatattcc ttgtttacga ttaataacta actttttcaa tttttgaact
     1141 tgcaatggca taataaattg aggaacatca taccctccta atacttgtaa ttctgtattc
     1201 gaattactaa caaaattatt tttattaggt cctagaacat ttccgtatat ggcaattcta
     1261 ttgttgccta aaataagtga accttgcgtt attgtaatat tacgaacaga aacattcgta
     1321 ttaagagtta tagtataacc attacatatt tcaacatcat catttggaga attaggtatg
     1381 ttgttcccta accatgtaga aggattattc caattacccg aagaaatagt ttggaattga
     1441 gaaaaagcat aattccaaat tagtaataat actattgaaa caaaaggttt catttgagta
     1501 attgttataa atatgcatat acagttttgt tttttattta cttatacgct ttgtttaaaa
     1561 taagtgttta ttttttttc
//
LOCUS       DAOTOW010000601         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_61818_length_1578_cov_2.544977, whole genome shotgun sequence.
ACCESSION   DAOTOW010000601 DAOTOW010000000
VERSION     DAOTOW010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61818_length_1578_cov_2.544977"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="P5243_09630"
     CDS             complement(<1..370)
                     /locus_tag="P5243_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19753.1"
                     /translation="MRFLSISICIILFQYSFSQSIGGGLAGNIQTESIGVELRAIVPT
                     KFFSVTPQIVYYPFFNTITELYAGIGIQVPFMHIKTANVYALGHASYDAWFNYDVSPM
                     LNAKFHNFAGEIGLGIISDRC"
     gene            569..1578
                     /locus_tag="P5243_09635"
                     /pseudo
     CDS             569..1578
                     /locus_tag="P5243_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT          569 a          224 c          286 g          499 t
ORIGIN      
        1 aacatcggtc tgaaataatt cctaatccaa tttcgccggc aaaattgtga aattttgcat
       61 ttagcatagg tgatacatca taattaaacc aagcatcgta agacgcgtgt cctaaggcat
      121 atacattagc tgttttaata tgcataaaag gaacttgaat gcctattccg gcatatagtt
      181 cagttattgt attaaaaaaa ggatagtata ctatttgcgg tgtaacagaa aagaattttg
      241 ttggtactat tgctcgtaat tctacaccaa tgctttctgt ttgaatgtta cctgccaatc
      301 ctcctcctat agattgagag aaagagtatt gaaaaagtat aatgcaaata cttattgata
      361 gaaatctcat aaattttttt tgataaaaat accggtttta attaagttga aatacatttt
      421 acgaggtaag attattaaca ttttttaaca acataaacaa tgaaaaaatc tttataactt
      481 attcataagt ttcaaattat aaataattgt atggtaggta aaaagaatta tatttgcacg
      541 attttaaaaa atacttgtca atttcacaat gaataaggcg tttaaagaat attcggggtt
      601 agatttgtct gcaataaaca aggaaatact tgctaagtgg atagaggaag atacatttaa
      661 acaaagtata cagagccgag aaggttctcc agcatttgta ttttatgaag gaccaccttc
      721 tgcaaatgga ttaccgggaa ttcatcatgt aatggctcgt gctattaaag atattttttg
      781 taggtttaaa actatgcaag gatttcaggt tcaccgtaaa gccggttggg atactcatgg
      841 cttgcctgta gaacttggtg ttgaaaaaga attaggaatt acaaaagaag atattggaaa
      901 aactattagt gttgaagatt ataataatgc ttgtagacac aatgtaatga agtatacaag
      961 tgaatgggaa caattaacac aaaaaatagg atattgggtt gatatgaaaa atccatatat
     1021 aacctatgat aataaatata tagaaacaat atggtatttg ttaaaacagt tgtatgaaaa
     1081 aggattgttg tacaaaggat acacaataca accttattca cctgctgccg gtaccggctt
     1141 aagttctcat gaattaaatc aaccgggatg ttatagagac gtaaaagata ccacttgtgt
     1201 agctcagttt aaagtgatac gaaatgaagt ttctcaatgg ctatttcctc aaaatgattc
     1261 acaattattt atacttgctt ggacaacaac accttggact ttgccttcta atacggcatt
     1321 gtgtgtaggc cctaaaatta cctatgtgca ggtacaaacg tttaatcctt atacagcaga
     1381 gcctattata gttgttttag caaaagaacg gctataagca tatttttcgg gaaaaggatt
     1441 agaagcatca atagatacat attctgcagg agaaaaaata gtaccatatc gtgttttaca
     1501 agaatttacc ggtgatgcat tgtgtggaat ttcatatgaa caacttatac cgtgggttgt
     1561 gcctcaaggt aaagcatt
//
LOCUS       DAOTOW010000602         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62021_length_1574_cov_4.657011, whole genome shotgun sequence.
ACCESSION   DAOTOW010000602 DAOTOW010000000
VERSION     DAOTOW010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62021_length_1574_cov_4.657011"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..774
                     /locus_tag="P5243_09640"
     CDS             64..774
                     /locus_tag="P5243_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19754.1"
                     /translation="MNQKISQLIHEIQQYIRENEALTNIHDIEKDILLEKIRDLYSAV
                     KLYPQLETIQTQNIVDTVSQKPMNEHIIELIEEPSITIQEESKPTIGEEIANSAIDIE
                     FETKQKIDTISTPVSEPAKQENVPTVEQKVETGEPKQSTVETNKPQPQIKADLGTKLA
                     KKPIANISQAIGINERFQFIKELFANDVTAYNSTIQTLNAMTHVDQAVEYISQHFNWD
                     MENATVQRFLSIVERRYL"
     gene            776..1447
                     /gene="rsmI"
                     /locus_tag="P5243_09645"
     CDS             776..1447
                     /gene="rsmI"
                     /locus_tag="P5243_09645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRS19755.1"
                     /translation="MPTLTVVPTPVGNLQDITLRAIETLKNATLILAEDTRTTGFLLK
                     HLGISCPMLSYHKFNEHSRVTQLIDRITNEPATVLVSDAGTPGISDPGTILVKACIEA
                     GITVSCLPGPTAFVPALVMSGFATDRFCFEGFLPHKKGRQTKLLQLQDEERTIILYES
                     PHRIVKTLEQILEFIGNRNVAIVREISKIYEEAIRGDVMSVIQHCKQKPLKGEIVLIL
                     EGNTK"
BASE COUNT          615 a          258 c          229 g          472 t
ORIGIN      
        1 acgtcaggta ataagtcagt attttcttcg tttgtagtag gaaaattata aaatcacatt
       61 attatgaatc aaaaaatttc acaattaata cacgaaattc agcaatacat tagagaaaat
      121 gaagcactta ctaatataca cgatatagaa aaagatatac ttttagaaaa aattcgtgat
      181 ttatatagtg ctgttaagtt atatcctcaa cttgaaacta ttcaaactca aaatattgta
      241 gatactgttt cacaaaaacc tatgaatgaa cacattattg aactcattga agaaccaagt
      301 attacaatac aagaagaatc aaaacctact ataggcgaag aaattgcaaa ttctgcaatt
      361 gatatagaat ttgaaacaaa acaaaaaatt gatacaataa gtactcccgt ttctgaacct
      421 gccaaacaag aaaatgttcc gactgtggaa caaaaagtag aaaccggtga accaaaacaa
      481 tcaactgttg aaacaaataa acctcaacct caaatcaagg cagatttagg taccaaatta
      541 gcaaaaaaac caatagcaaa tatttctcag gctattggta taaatgaacg tttccaattt
      601 ataaaagaac tatttgcaaa tgatgtaact gcatataatt ctactattca aacacttaat
      661 gctatgacac atgttgacca agctgttgaa tacatatcac aacattttaa ttgggatatg
      721 gaaaacgcta cagttcaacg atttctatct attgtagaac gaagatattt ataatatgcc
      781 tacacttacg gtagttccta caccggtagg aaatttacaa gacataacac tgcgagctat
      841 agaaacactc aaaaatgcta ccttaattct tgccgaagat acccgcacta ccggtttttt
      901 actaaaacat cttggcattt cgtgtcctat gctatcatat cataaattta atgaacattc
      961 cagagttaca caacttattg acagaattac aaacgaacct gctaccgttt tagtttctga
     1021 tgcgggtact ccgggaatat cggaccccgg tactatatta gttaaagcat gcattgaagc
     1081 aggtatcact gttagttgct taccagggcc aacagctttt gtacctgcat tggttatgag
     1141 tggttttgca actgacagat tttgttttga aggtttttta cctcataaaa aaggaagaca
     1201 aacaaaatta ctgcaattac aagatgaaga acgtacaatt attttatatg aatcacctca
     1261 tagaattgta aaaacgttgg agcaaatttt agaatttata ggcaatagaa atgttgccat
     1321 agtacgtgaa atttcaaaaa tttatgaaga agccattcgt ggtgatgtaa tgtcggttat
     1381 tcaacattgt aaacaaaaac ctctcaaagg tgaaatagta ttaatacttg aaggaaatac
     1441 aaaataattt atctagctgt tgaatactca aaagatttca atgtagtctc aataattttc
     1501 taaaaaatta tatatttaca atcattattg tccgataaaa ctacaaaatt aatttaataa
     1561 aacaaaattt attt
//
LOCUS       DAOTOW010000603         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62348_length_1569_cov_2.898943, whole genome shotgun sequence.
ACCESSION   DAOTOW010000603 DAOTOW010000000
VERSION     DAOTOW010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62348_length_1569_cov_2.898943"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="P5243_09650"
     CDS             <1..1101
                     /locus_tag="P5243_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19756.1"
                     /translation="GIEEPKFSMISNIPNIDLNINKKQCEFDILDYYTGDTPTNVSAV
                     LLNGSKAHVNIVESTISIEADEVCADFKDTVIVSVTNDYGILTKKIPVEYNHSIHLLP
                     PGIGIVTVDTLGTYLMLAWERPFSQTIEKYTIYREGLTNQYTKLAEQPYNQLSVYIDN
                     TALFNTRAYKYAITATDVCGNESPKSDAHKSIHLQRKYVNNELQLNWTLYEGAEVLGY
                     QLLKGTTHTNMVAVDEFATDQTSYTVDNPGNYLYRIGTVMKHAVSPEVLKIQSGPFVL
                     AMSNIAEANTMISSPKIEATCSIQNGTIQVQCAEALQNACVELISSTGTVVQSRTVNG
                     SEFEISCKGVAKGVYFVSITTSNQSVQIPVTL"
     gene            1160..>1569
                     /locus_tag="P5243_09655"
     CDS             1160..>1569
                     /locus_tag="P5243_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRS19757.1"
                     /translation="MNHILRVGLVQQSVTDVVEQNKQLLVHNIGECAKQGAQLIVLQE
                     LHNTVYFCQTEDVIQFDLAETIPGPSTELYSSIAKQYGIVLVTSLFEKRAAGIYHNTA
                     VVFDTDGIIAGMYRKMHIPDDPAYYEKFYFTPGD"
BASE COUNT          576 a          242 c          272 g          479 t
ORIGIN      
        1 ggaatagaag aacctaagtt ttcaatgatt agtaatatac caaatataga tttaaatata
       61 aataagaaac aatgcgagtt tgatatatta gattactata ccggcgatac tcctaccaat
      121 gtttctgcag tattgcttaa tggcagcaaa gctcatgtaa atatagtaga atcaaccata
      181 tcgattgaag cagatgaagt ttgtgccgat tttaaagata ctgttattgt aagtgttact
      241 aatgattacg gaatattaac caaaaaaata cctgtagaat ataaccattc tatacactta
      301 ctacctcccg gaataggtat agtaactgtt gacacattag gtacatatct tatgcttgcg
      361 tgggaacgac cattttcaca aactatcgaa aaatatacta tttaccgcga gggattgacc
      421 aatcaatata caaaacttgc tgaacaacca tacaatcaat taagtgtata tatagataac
      481 actgcgttgt ttaataccag agcatacaaa tatgctataa ctgctaccga tgtatgcggc
      541 aatgaatcgc ctaaatctga tgctcataaa tctatacatt tgcaaagaaa atatgtaaat
      601 aacgaattgc aactcaattg gacattatac gaaggcgcag aagttcttgg atatcaattg
      661 ttgaaaggaa caacacatac aaatatggta gctgttgatg aatttgcaac cgatcaaaca
      721 tcgtatacag ttgataatcc gggtaattat ttgtatagaa taggtacagt tatgaaacac
      781 gctgtttctc ctgaagtttt aaaaattcaa agtggaccgt ttgtacttgc aatgagtaat
      841 atagccgaag ctaatacaat gatatcttca ccaaagatag aggctacttg tagtattcag
      901 aatggtacaa tacaagtaca atgtgctgaa gcattacaaa atgcttgtgt agaattaatt
      961 tcatctacag gaactgttgt gcaaagcaga acagtaaacg gttctgaatt tgaaatttca
     1021 tgcaaaggtg tagcaaaagg tgtatatttt gtttctatta ctacatctaa tcaatctgta
     1081 caaatacctg ttacattata gatactagcc ttgtacaatt gatttcaaaa aaaatatgta
     1141 tttttgaaaa aaaatactta tgaatcatat tttgcgtgtt ggacttgttc agcaatctgt
     1201 tacagatgtt gtagaacaaa ataaacaact attggtacac aatataggtg aatgtgcaaa
     1261 gcaaggtgca caattaatag ttttgcaaga attacacaat acggtatatt tttgtcaaac
     1321 agaagatgta atacagtttg atttagcaga aacaatccct ggtccgtcaa cagaattgta
     1381 tagttcaata gctaaacaat atggaatagt attggtaacg tcattgtttg aaaaacgagc
     1441 agcaggaatt tatcataata ctgccgttgt atttgatact gacggaataa tagccggaat
     1501 gtatcgcaaa atgcatatac ccgatgatcc ggcatattat gaaaaatttt attttactcc
     1561 gggtgatat
//
LOCUS       DAOTOW010000604         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62530_length_1566_cov_3.518200, whole genome shotgun sequence.
ACCESSION   DAOTOW010000604 DAOTOW010000000
VERSION     DAOTOW010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62530_length_1566_cov_3.518200"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="P5243_09660"
     CDS             <1..1421
                     /locus_tag="P5243_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19758.1"
                     /translation="IVSFDTLFMCNTGNKYADYILNKDIFSFSKGNFNFSINPACNFE
                     YSQDLDDTVNGWTNTRGLIIESDITNKVHVFTAFYETQSRFRDYRNDRIEELGQRTIP
                     GQSRAKPFGDDNQADDYAFADAYISFNPDSIFGFQFGHGKHFIGDGYRSLLLSDNAMN
                     YPYFKITTNIWNIQYVNMWSQQYYVDFPHAPNSRYPKKWNVMHYLDWSVTKWFNIGIF
                     ETIIWQNEDSSGVYRGFEFNYLNPVIFLRPVEFSVGSPDNVLMGLTGKVTLWKNHVFY
                     GQLAIDEFKISEMKARNGWWGNKYALQAGYKTFDIAGVKHLDFQTEVNYVRPFMYSHF
                     TYSQQYGHALQSMAHPRGSNFYESVSFIKYNYKRLFIEAKYLYLVHGQDTVGSNFGNN
                     IFKLYTTRSQEYNNKTGKSAIENIVQYKDITVSYLVHPPSNTTISAGISNRSAISELN
                     SKQQMMYFIALRSSLQNFYYDF"
BASE COUNT          543 a          218 c          276 g          529 t
ORIGIN      
        1 aaattgtatc atttgataca ttatttatgt gtaataccgg taataaatat gcagattata
       61 ttctcaacaa agatatattt tcattttcaa aaggtaattt taatttttct attaatcctg
      121 cctgtaattt tgaatattca caagatttag atgatacagt gaatgggtgg acaaatacac
      181 gcggccttat aatagaatca gatattacaa acaaagttca tgtgtttaca gctttttatg
      241 aaacccaatc aaggtttaga gattatcgta acgacaggat agaggaatta ggacaacgaa
      301 caataccggg acaaagtcgt gctaagccat ttggcgatga taatcaggct gatgattatg
      361 cttttgccga tgcatatata tcttttaatc ccgattcaat ttttggattt caatttgggc
      421 acggtaagca ttttattggc gatggatata ggtcattgtt gctctcggat aatgctatga
      481 attatccgta ttttaaaata acaacaaata tttggaacat acaatatgtc aatatgtggt
      541 cgcaacaata ttatgttgat ttccctcatg ctccaaattc acggtatcct aaaaaatgga
      601 atgttatgca ttatttagat tggtctgtaa ctaaatggtt caatattggc atttttgaaa
      661 ctataatatg gcagaatgaa gattcttcgg gagtatatcg tggttttgaa tttaattact
      721 taaacccggt tatttttttg cgtcccgttg aattttctgt tgggtcgccc gataatgtgt
      781 taatgggatt aacaggaaaa gtaacactct ggaaaaatca tgtattctat gggcaattag
      841 caattgatga attcaagatt tctgaaatga aagcccgaaa cggatggtgg ggtaataaat
      901 atgcattaca agcagggtat aaaacgtttg atattgctgg tgttaaacat ttggattttc
      961 aaacagaagt aaattatgtg cgcccgttta tgtattcaca ttttacatat tcacaacagt
     1021 atggtcatgc attacaatca atggcgcatc cacgcggttc taatttttat gaatcggtga
     1081 gttttatcaa atataattat aaacgattgt ttatagaagc taaatatttg tatttggtac
     1141 atgggcaaga tacggtgggt tctaattttg ggaataatat atttaagttg tacacaactc
     1201 gtagccaaga atataataat aaaacaggta aatcagccat agaaaatata gtacaatata
     1261 aagatattac agtttcgtat ttagttcacc cgccaagtaa tactactata tctgccggaa
     1321 ttagtaatcg ttctgcaatt agtgaattga attcaaaaca acaaatgatg tattttatag
     1381 cattacgatc gtcattgcaa aatttttatt atgattttta agtacataat caaaaaaaag
     1441 taattttgcc ttattaatat tatatgtatg aattatagag agcgattgca gcataaaaaa
     1501 agaatagtga taaaaatagg aacatcatca ctaacattcc caaacggcag attaaattta
     1561 acacgt
//
LOCUS       DAOTOW010000605         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62698_length_1563_cov_3.476127, whole genome shotgun sequence.
ACCESSION   DAOTOW010000605 DAOTOW010000000
VERSION     DAOTOW010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62698_length_1563_cov_3.476127"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="P5243_09665"
     CDS             <1..411
                     /locus_tag="P5243_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19759.1"
                     /translation="SKITKNNCMDSQLYSFISDFRNFEAMVPSEHRNKANFSQHSVTI
                     EVMQGMNITLQIIEEEPYKLIKLGSEQNKEFCIWIQLKQVAPYDTRIRITLHADIPLM
                     AKMFAKNKLQVFVDGFAEALAQIPVYAFQGNNLN"
     gene            complement(513..1148)
                     /locus_tag="P5243_09670"
     CDS             complement(513..1148)
                     /locus_tag="P5243_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRS19760.1"
                     /translation="MKISGFLKQSLIDYPGKIAAVVFTQGCNFQCGYCHNPQLVLPHL
                     FIENSEYNPSNIFRYLKSRKNWLDGVVITGGEPTIHKDLPEFLKKIKAMGYSIKLDTN
                     GSNPYMLKQIITNKLVDYIAMDIKNIPEKNLYEEIIGIQMTTKIIENLQLSIQIINNS
                     SIEREFRTTFIPKIHNKNTIELIKKLVGKNICYKVNEFREGETIEKELHTN"
     gene            complement(1145..>1563)
                     /gene="nrdD"
                     /locus_tag="P5243_09675"
     CDS             complement(1145..>1563)
                     /gene="nrdD"
                     /locus_tag="P5243_09675"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRS19761.1"
                     /translation="KGAKPYYTNSTQLPVNYTDDLFEALTMQDALQTKYTGGTVFHAF
                     LGEQQLPIDTVKTLIKKITATFHLPYITLSPTFSICPEHGYIFGEHKQCPKCAEIGQT
                     TDCEIFSRIVGYLRPIKQWNDGKQEEFADRKLFDKL"
BASE COUNT          503 a          228 c          255 g          577 t
ORIGIN      
        1 agtaaaatta caaagaacaa ttgtatggat tcgcaattgt attcttttat atctgatttt
       61 cgaaattttg aagctatggt tccgtctgaa catagaaata aagccaattt ttcgcagcat
      121 tcggtaacta ttgaagtaat gcaaggtatg aacataacat tacaaataat agaagaagaa
      181 ccgtataaac ttataaaatt aggatcggaa caaaataaag aattttgtat ttggattcaa
      241 ttaaaacaag ttgcacctta cgatactcgc atacgaatta cacttcatgc agatatacca
      301 ttgatggcca aaatgtttgc gaaaaataaa ttgcaagttt ttgttgatgg atttgccgaa
      361 gcattggctc aaattcctgt atatgcgttt cagggtaata atttaaatta attttaatct
      421 atttgttatt ttaattcaaa aaacaatgtt gaacagcagt ttctatagaa atttaaaaag
      481 ttttttggga aaattcccaa gcttgtttat atttaattag tgtgaagttc tttttctatc
      541 gtttctccct ctctaaattc attaacttta taacatatat ttttaccaac aagcttttta
      601 ataagttcaa tagtattttt gttgtgtatt tttggaataa atgtcgttcg aaattctctt
      661 tctatagaac tattattaat aatttgaatg gaaagctgta aattttctat aattttagta
      721 gtcatttgaa tacctattat ttcttcatat agattttttt cggggatatt tttaatatcc
      781 attgcaatat aatcaactaa tttatttgta attatttgtt tcagcatgta tgggtttgag
      841 ccgttagtat ccagtttaat tgagtagccc atagctttaa ttttttttag gaactcaggt
      901 aaatctttgt gaatagtagg ttcgccacct gttattacta ctccgtcgag ccaattttta
      961 cgacttttaa gatagcgaaa tatgttactt ggattatatt cagaattttc aataaaaaga
     1021 tggggtaata caagctgtgg attgtgacaa tatccacact gaaaattgca gccttgtgta
     1081 aacacaacag ctgcaatttt accgggatag tcaataagac tttgtttcaa gaaaccacta
     1141 attttcataa tttatcaaat aatttacggt cagcaaattc ttcttgttta ccatcgttcc
     1201 attgtttaat agggcgtaaa taccctacaa tacgagagaa aatttcacaa tcggttgttt
     1261 ggccaatttc agcacattta ggacattgtt tgtgctcgcc aaaaatgtac ccatgttcag
     1321 ggcaaatgct aaaagtgggt gacagtgtta tgtacggcaa atgaaacgtt gcagtaattt
     1381 tttttatcaa ggtttttact gtatcaatgg gcaattgttg ttcccctaaa aatgcatgaa
     1441 atactgtgcc acctgtgtat ttggtttgca aagcatcttg cattgtcaag gcttcgaaca
     1501 aatcatcagt ataattaact ggtaattgtg tagaattggt gtaatatggt tttgctcctt
     1561 tac
//
LOCUS       DAOTOW010000606         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62702_length_1563_cov_3.398541, whole genome shotgun sequence.
ACCESSION   DAOTOW010000606 DAOTOW010000000
VERSION     DAOTOW010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62702_length_1563_cov_3.398541"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="P5243_09680"
     CDS             <1..163
                     /locus_tag="P5243_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HRS19762.1"
                     /translation="LLRLHSHPEDEIDKVTTPRGITIAGLNEMEHNGFSSALIKGIVK
                     SYDKATQKK"
     gene            180..755
                     /locus_tag="P5243_09685"
     CDS             180..755
                     /locus_tag="P5243_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19763.1"
                     /translation="MRKLLVWIVCTTLLAVACTEKKPQIGIAQLAEKSQEYQTKIASL
                     ESRLNPQDIDAFMKINDSITLVQQQADSVLGALFASITDTIVLDFTQTKNDHRIKIQK
                     VWLTGAKYNELHIQALVKAIENSSFMGPYTSATMIDNTGKKIEVGGGIGGAPDTKLVA
                     GQVYTFTGTIDNLHLLANLKQLQFDEEIKKW"
     gene            1191..>1563
                     /locus_tag="P5243_09690"
     CDS             1191..>1563
                     /locus_tag="P5243_09690"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS19764.1"
                     /translation="MPKIALITGVTGQDGAYLTEFLLKKGYIVHGIKRRSSLFNTDRI
                     DHLYQDPHVDNRNLILHYGDLTDSMNLTKIIQEVQPDEIYNLAAMSHVKVSFDMPEYV
                     ANADGIGTLRILEAVRLLGLTK"
BASE COUNT          564 a          264 c          264 g          471 t
ORIGIN      
        1 gttactccga ttgcattcac accccgaaga cgaaattgac aaggttacaa ccccccgcgg
       61 tattactata gcaggactta atgaaatgga acataacgga ttcagttctg ccctaataaa
      121 aggtattgta aaatcgtatg acaaagcaac tcaaaaaaaa taatctaaaa aatatgaata
      181 tgagaaaatt acttgtatgg attgtatgca ctaccctatt ggcagtagca tgtaccgaaa
      241 aaaaaccgca aattggcata gctcaattag ctgaaaaatc acaagagtat caaacaaaaa
      301 ttgcaagtct tgaatcgcgt ttaaatcctc aagatatcga tgcatttatg aaaataaatg
      361 attctattac cttagttcaa caacaagcag acagtgtatt aggagcttta tttgcatcga
      421 ttaccgacac gatagtgtta gactttacac aaacaaaaaa cgatcaccgt ataaaaattc
      481 aaaaagtatg gcttaccgga gctaaataca acgagctaca tattcaagcg ctggttaaag
      541 ctatagaaaa cagtagtttt atgggaccat ataccagtgc tactatgata gataatacag
      601 gcaaaaaaat tgaagttggc ggtggtattg gtggggctcc agatactaaa ttagttgccg
      661 gccaagtata tacatttacc ggaactatcg acaatctaca tctgcttgct aatctcaaac
      721 aattacagtt tgacgaggaa attaaaaagt ggtaaaggac aaaatgtctg attgtcgaaa
      781 agtcggaaaa gtctaaaaag tcttcaaaaa ttttacaata tgtaagtata aaatattgca
      841 cgattacata tcaccccttc ggggttttat ttgtatgcta tatatgtttt ctataataat
      901 attagccttt caggctttaa aaaaattctg aataggctat tttttacaat cttgaaagtt
      961 ataattttgt ttcaaaaatt aacaaggcta aaaccccgaa gaggtgatat tattatagaa
     1021 taaaataaaa aaaacaccat caaaaaccct gtaggggtaa cattattttt tgaaacatat
     1081 ctatttattg aatgcctggg ctacattaca atatacaatt atttcgattt ttctacatgc
     1141 aatttgtata aattccttac ttttgttttt aatacaatat ttcatttatt atgcctaaaa
     1201 tagctcttat taccggtgtt accggacaag atggtgcata tctcaccgaa tttttattaa
     1261 aaaaaggata cattgttcac ggtatcaaac gtcgttcgtc gttattcaat accgatagaa
     1321 tcgaccattt gtatcaagac ccgcatgtag ataatcgtaa tttgattctg cattatggcg
     1381 atttaaccga ttctatgaat cttactaaaa taattcaaga ggtgcaaccc gacgaaatat
     1441 ataacttggc tgctatgagc catgtgaaag taagtttcga catgcccgaa tatgttgcca
     1501 atgccgatgg tataggcaca ttgcgcattt tagaagctgt acggttgttg gggttaacta
     1561 aaa
//
LOCUS       DAOTOW010000607         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62706_length_1563_cov_3.051724, whole genome shotgun sequence.
ACCESSION   DAOTOW010000607 DAOTOW010000000
VERSION     DAOTOW010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62706_length_1563_cov_3.051724"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="P5243_09695"
     CDS             complement(<1..1548)
                     /locus_tag="P5243_09695"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19765.1"
                     /translation="MPEPTPIGYFELKKPSTPVQVSVSGIVTTPIAGSVQWLNESGTV
                     ISTSNPYVTGLNSNTEIKTYAIARQKIGNCYSDTIQVPIQIIDCPVPSPQVNISPSAT
                     ICNYDAVPQIQTSIGSTWLQGTRPTPVIPVEFRLYSQATGGVAISSNTTGNFTPVLAN
                     MSQGGNYDFWIAEYNPNVIPQGCESVLRTKVTIQVNKTPAVGIVGQKDVCEQTANAQL
                     QIKDVLPNAVVEWYTSMPNIPSVTPPVAQGMYYTPSVTNVGTHTVYAVQRLNGCMSEL
                     AQVSFTIKPLPAPPVTLPASVCQGQPHVALQATGTGITWYQNANKTGVKLLTNSNTYI
                     PTVDNTTTFYASQTVNECESSTAPVVYTVKSIPAKPIIQTKDKSCDTETEHILSVTPE
                     TGSVIRWYSSPDTSSYLTSGNEYKHQIQGVSGQKTYYARKFVDGCISDVSSKYFTIVP
                     TPQPPVITNTTVCKQSNATLISDDNDNEWYSDQTLQTMVFKGRNYTVNQVISNQTLYV
                     IKNKFTCT"
BASE COUNT          447 a          278 c          291 g          547 t
ORIGIN      
        1 tgtacaagta aatttatttt taattacata tagagtctga tttgaaatca cttgatttac
       61 agtgtaattt ctgcctttaa ataccatggt ttgaagcgtt tgatcgctat accactcatt
      121 gtcattatca tcacttataa gagtagcatt actttgttta catacagtag tatttgttat
      181 aacaggtggt tgtggtgttg gaacaatagt aaaatatttt gaacttacat cacttataca
      241 accatcaaca aatttacggg cataatatgt tttttgtccc gaaacacctt gaatttgatg
      301 tttatactca ttacccgaag ttaaatacga cgaggtgtcg ggtgaactat accaacgaat
      361 tactgaacct gtttcgggag ttacactcag tatatgttct gtttccgtat cgcatgattt
      421 atctttagtc tgaattatag gttttgcagg aatagatttt accgtatata caacaggtgc
      481 tgtactactt tcacattcat ttactgtttg cgaagcgtag aacgtagttg tgttatcaac
      541 tgtaggtata tacgtattac tatttgttaa taatttaaca cctgttttat tcgcattttg
      601 ataccaagta ataccggttc ctgttgcttg aagtgctaca tgaggttgtc cttgacatac
      661 cgatgcaggt aaagtaacag gtggcgcagg caaaggttta atagtgaaac ttacttgtgc
      721 aagttcactc atacacccat tgagtcgttg tactgcatat acagtatgag taccaacatt
      781 ggtaacactt ggcgtataat acatgccttg tgcaaccggt ggcgtaaccg atggaatatt
      841 tggcatacta gtataccatt caactacagc atttggtaat acatctttta tttgtaattg
      901 tgcatttgct gtttgttcac acacatcttt ttgaccaact attcctaccg caggggtttt
      961 atttacttgt atcgttactt ttgttcgcaa tacactctca cagccttgcg gaattacatt
     1021 aggattatat tctgcaatcc aaaaatcata attacctcct tgcgacatgt tggctaatac
     1081 aggagtaaaa ttgcctgtag tattcgaact tattgcaact cctccggttg cttgtgaata
     1141 caatctgaat tctacaggta ttaccggagt aggacgagtg ccttgtaacc atgtagaacc
     1201 tatactagtc tgtatttgtg gtacagcatc gtaattacaa atagttgcac tagggcttat
     1261 gtttacttgt ggcgatggaa ccggacaatc aattatttga ataggaactt gaatagtatc
     1321 gctataacaa tttccaattt tttgacgagc aattgcatat gttttaattt cagtattaga
     1381 atttaatccg gtaacatatg gattgcttgt agagattacc gttcctgatt cattcaacca
     1441 ttgtactgaa cctgctatag gagtagttac tatacccgaa actgaaactt gaacaggagt
     1501 gcttggtttt tttagttcaa aatagccgat aggcgttggt tcgggcacat agtttatacg
     1561 cac
//
LOCUS       DAOTOW010000608         1560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62890_length_1560_cov_2.892359, whole genome shotgun sequence.
ACCESSION   DAOTOW010000608 DAOTOW010000000
VERSION     DAOTOW010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62890_length_1560_cov_2.892359"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..926)
                     /locus_tag="P5243_09700"
     CDS             complement(207..926)
                     /locus_tag="P5243_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS19766.1"
                     /translation="MSIIRIGIILCLSFAYSKVIAQKPTVYLFPGQGSDYRIFDSIQF
                     DSSYSVVHIQYAIPSKGISLREFSYSLISQIDSARPIILIGVSMGGMICCELESVLNP
                     IKTIIISSAKCSNELPFRYTFQRYIPIYKLFPKRILYWGALLMQPLVEPDRKVCKATF
                     KAMLKSKHPLYLKRTIAMIVTWDKYEYNTKSIIHIHGNNDHTLPVRNITADYFIHNGS
                     HMMTLTRGHEIETILKQLLEH"
     gene            complement(931..>1560)
                     /locus_tag="P5243_09705"
     CDS             complement(931..>1560)
                     /locus_tag="P5243_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRS19767.1"
                     /translation="TAGVHADGDKKGNLYCTELMPERFGRVRKYALGKTSGKANIEKN
                     LEELGLELEPEIMKLVTQKVVELGDKKETITTEDLPYIVREVLGTTQIEEKVYIENYY
                     VCNAKNLKPVATIKLNVNGEVFEETSAGDGQYDAVMNALLKIYSHLHIQLPKLTDYLV
                     TIPPGGKTDALVETIITWETENGTFKTRGLDCDQTAAAIKATVRMLNMI"
BASE COUNT          512 a          272 c          241 g          535 t
ORIGIN      
        1 agcatgttca aaattgcatg cttgaagttc acgcacaacc ttttttgctt gtatttttaa
       61 taaatacgaa cctataaatt cctgaacaaa ttgcaatttt ctcatactgc aataatacaa
      121 ttttttcacg atgaatgaaa aacaaaatgc cgaaaaaaat atataacgac ggtttaaggt
      181 ttagagaaac acgtttaggg tatttttcaa tgctcaagaa gttgttttaa aatagtttca
      241 atttcatgtc cacgagtcaa tgtcatcata tgcgaaccat tgtgaataaa ataatccgca
      301 gtaatatttc gtacaggcag tgtatggtcg ttattgccat gaatatgaat aattgacttt
      361 gtattatatt cgtacttatc ccaagtaaca atcatagcaa tagtacgctt taagtacaaa
      421 ggatgtttac ttttaagcat tgccttaaaa gtagctttac aaacttttct atccggttct
      481 accaagggtt gcattagtaa tgccccccaa tacagtattc gttttggaaa taatttgtat
      541 ataggtatat accgctgaaa tgtatagcga aacggtaatt cgttagaaca tttagcactt
      601 gaaattataa tagttttaat tgggtttaat acagactcta attcacaaca aatcataccg
      661 cccatagaaa caccaatgag tataattgga cgggcgctat ctatttgaga aatgagagaa
      721 taagaaaatt cacgaagcga tattcctttt gaaggaattg catactgaat atgcactacc
      781 gaatacgatg aatcaaattg aatagaatca aaaattcggt aatctgatcc ttgtcccggg
      841 aaaagatata ctgtgggttt ttgagcaata actttggaat aagcaaaact taaacatagt
      901 ataataccta ttcgtattat gctcatacaa ttatatcata tttaacatac gtacagtcgc
      961 tttaattgca gctgccgttt gatcacaatc taaaccacga gttttgaaag tgccattttc
     1021 agtttcccaa gtaataatag tttctaccaa cgcatcagtt tttcctccgg gaggaattgt
     1081 aaccaaataa tctgttaatt ttggtaattg tatatgtaaa tgagaataaa ttttgagtaa
     1141 cgcattcata acggcatcat attgtccatc accggcactg gtttcttcaa aaacttcacc
     1201 atttacattg agtttaatag tagctacggg ttttaaattt tttgcattgc atacataata
     1261 attttcaatg tacacttttt cttctatttg agtagtacca agaacctcac gaacaatata
     1321 gggtaaatct tcggttgtaa tagtttcttt tttgtcaccc aattctacaa ctttttgggt
     1381 aaccaatttc ataatttcag gttcaagttc taatcctaat tcttcaagat ttttttcaat
     1441 attagctttg cccgatgttt ttccgagagc atattttctt actcttccaa accgttcggg
     1501 cataagttcg gtacaatata aattaccttt tttatcacca tcggcatgta ctcctgcagt
//
LOCUS       DAOTOW010000609         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_62936_length_1559_cov_3.119681, whole genome shotgun sequence.
ACCESSION   DAOTOW010000609 DAOTOW010000000
VERSION     DAOTOW010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62936_length_1559_cov_3.119681"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..572
                     /locus_tag="P5243_09710"
     CDS             87..572
                     /locus_tag="P5243_09710"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19768.1"
                     /translation="MKSFPHFFIKFSIIISISISVFLFSCDNKTVYKEFIKFPDLEWA
                     KNDIKTFIFENTDDVSKVDVYLAFRYAQGFQFDIMLFSVIEKSPVGNFPYPISIQIKD
                     DNGKYIGEGSGDIWDIEIPIKQNVSLAKGTYRYEISSNMPLQKLNMIMEVGLIIKKSN
                     Q"
     gene            695..>1559
                     /locus_tag="P5243_09715"
     CDS             695..>1559
                     /locus_tag="P5243_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRS19769.1"
                     /translation="MKNLALVSIAIIINIATYAQEIVTNGEISDVIVYLQGASITTKS
                     KTQIPEGVSTVVITKQPQSIEPQSIQVSGKGNFTILGVQFRTNYLEKQQQEKQVLELS
                     EKRKLIDNTLKLATEEINVYKTETQLLEKNMEIKGANAVLVTSELEKTANFYRSRLLD
                     IKKKIIEAQNKQTDLQTQLKDIDKQLYEIKAKAEQYIGEIVVTVSSKTQQTIQLESNY
                     YVNDAGWVPQYDIRVKDVQSPVALTYKASIYQNTGISWKNIQVRLSTGNPTLSGNVAI
                     LSPWILQREQEF"
BASE COUNT          654 a          218 c          224 g          463 t
ORIGIN      
        1 aaataaattt tgttttatta aattaatttt gtagttttat cggacaataa tgtttagcaa
       61 tatataaact ataaaaacct atacctatga aatcatttcc tcattttttt ataaaattta
      121 gtataataat ttcaatatct atatctgtat ttcttttttc ttgtgacaat aagacggtat
      181 ataaagaatt tataaaattt ccggatttag aatgggcaaa aaatgacata aaaacattta
      241 tatttgaaaa taccgatgat gtttctaaag tagatgtata tttagcgttt cgatatgcac
      301 aaggatttca atttgacata atgctattta gtgtgataga aaaatcaccg gttggtaatt
      361 tcccatatcc tataagtatt caaataaaag acgataatgg taaatatata ggcgaaggca
      421 gtggcgatat ttgggatatt gaaataccaa taaaacaaaa cgtttcttta gcaaaaggaa
      481 catatagata tgaaatttca tcaaacatgc cattgcaaaa actaaatatg ataatggaag
      541 ttggtttgat tattaaaaaa tcaaatcaat agaaaaaact atacagaaaa aaatacgtca
      601 agttactttc caatatttgg tatattaatt gcaaaatcat tttgtcaaaa aaacagatta
      661 ttacaattat tgtatatatt aaaaaaaata ttttatgaaa aatttagcat tagtaagcat
      721 tgcaattatt ataaacattg caacatatgc gcaagaaatt gttacaaacg gtgaaattag
      781 cgatgtaata gtatatttac aaggagcatc aatcacaacc aaatcaaaaa cacaaatacc
      841 cgaaggtgta agtaccgttg ttataacaaa acaaccgcaa tctattgaac cacaaagcat
      901 tcaagtttca ggaaaaggaa acttcacaat tttgggagta cagtttcgca caaattatct
      961 cgaaaagcaa caacaagaaa aacaagtatt ggaactctcc gaaaaacgaa aactcattga
     1021 caatacctta aaattagcaa cagaagaaat aaatgtgtat aaaaccgaaa cacaattact
     1081 cgaaaaaaac atggaaatta aaggtgctaa tgctgtatta gttacatcag aacttgaaaa
     1141 gacagcaaac ttttatcgaa gtagattatt ggatataaaa aagaaaatta tagaagctca
     1201 aaataaacaa acagacttac agacacaact taaagacatt gacaagcaac tatatgaaat
     1261 aaaagctaaa gcagaacaat atattggaga aattgtagta acagtttcat ctaaaacgca
     1321 acaaacaatt caattagaaa gtaattatta tgtaaatgat gccggctggg ttccgcaata
     1381 tgatattcgt gtaaaagacg ttcaaagtcc ggttgcactt acctataagg catctattta
     1441 tcaaaacacc ggtatttcat ggaaaaatat tcaagttcga ctttcaacag gtaatccaac
     1501 acttagtggt aatgttgcta tactttcgcc ttggatactt caacgtgaac aagaatttg
//
LOCUS       DAOTOW010000610         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_63140_length_1556_cov_3.044637, whole genome shotgun sequence.
ACCESSION   DAOTOW010000610 DAOTOW010000000
VERSION     DAOTOW010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63140_length_1556_cov_3.044637"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="P5243_09720"
     CDS             complement(<1..875)
                     /locus_tag="P5243_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19770.1"
                     /translation="MKKILLVLGIGVASFAAVAQQQNGLERIIVEKYYISDENDQQCD
                     EGELPAGSVTYRIYVDMLPGYKFQTCYGDENHEMRFETTTKFFNNEKRGNSTPTFTFD
                     HAKKGTVLLDSYVTAGAACDGYYGIPKNLDNEIGTLVNSDGYLQASFDAAGNAISVKD
                     GLLEADLNNYPMDVTTIGISTFLNAIKAQNATTNGQSLVVTNGAWAALGGAMGPTSEN
                     ILLIAQFTTNGTFSYKLNVQIGNPNGYDAENYVVSDPIGDEKTIASLQGTYEPVAISK
                     PTVSITSPSSGAKFKV"
     gene            complement(884..>1556)
                     /locus_tag="P5243_09725"
     CDS             complement(884..>1556)
                     /locus_tag="P5243_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19771.1"
                     /translation="EKYYVAQSKDVKDTVGGVLAKKSVTYRVYVDLLPGYKLQTVWGS
                     EEQPLFFKTSTYFYNNINGATTGDDLFDTISFKKNNVLLDSWISMGAVTRFYNAVLLS
                     EDVDGSIFNHKQALLQKDGLQYRDVYRTVYFNLDLTPLEKRTKSQIFATNNGAWANFM
                     GAQGATPENRILIAQFTTNGEFSFECNIQVGTPAGEAIQFVARNPQGEQVQHQSLIFS
                     SKHLK"
BASE COUNT          483 a          296 c          218 g          559 t
ORIGIN      
        1 actttaaatt tagctccgct tgaaggtgat gtaatactta cagtaggttt tgaaattgct
       61 acaggttcat atgttccttg taatgaagca attgtttttt catcaccaat tgggtcagac
      121 acaacataat tttcagcatc gtatccatta ggattaccaa tttgaacatt taatttatat
      181 gaaaaagtac cgttggttgt aaattgagca atcaataata tattttccga agttggtccc
      241 atagcaccac caagagcagc ccatgcacca tttgttacaa ctaatgattg tccatttgta
      301 gtagcatttt gagcttttat agcattcaaa aaagtactaa tacctatagt tgtaacatcc
      361 ataggataat tatttaaatc agcttccaat aaaccgtctt ttacacttat agcattaccc
      421 gcggcatcaa atgaagcttg caagtaacca tctgaattta caagggttcc tatttcatta
      481 tctaaatttt taggtattcc gtaatatcca tcacaagcgg ctcctgcagt aacatatgaa
      541 tctaataata ctgttccttt ttttgcatgg tcaaacgtaa atgtaggagt agaatttcct
      601 cgtttttcat tattaaaaaa tttagtagtt gtttcaaaac gcatttcatg attttcgtca
      661 ccatagcacg tttggaattt atatcccgga agcatatcta catatattct gtatgttaca
      721 gacccggcag gtaattcacc ttcatcacat tgttgatcat tttcatcaga aatataatat
      781 ttttcaacta ttattctttc caacccattt tgttgctgag ctacagcggc aaaacttgcc
      841 actcctatcc ctaatacaag taaaattttt ttcatcgttt gttttatttt aaatgtttac
      901 tactaaaaat taatgattga tgttgaactt gctctccttg cggattacga gctacaaatt
      961 gaattgcttc gcctgcagga gttcctactt gaatattaca ttcaaaacta aattcaccgt
     1021 ttgttgtaaa ttgagcaatt aaaattctat tttcgggtgt tgcaccttgt gctcccataa
     1081 agtttgccca agctccatta tttgttgcaa aaatttgaga ctttgtacgt ttttcaagag
     1141 gtgttaaatc taaattaaaa tataccgttc tatatacatc acgatactgt aatccatctt
     1201 tttgaagcaa tgcttgttta tgattaaaaa tacttccgtc aacatcttct gagagtagta
     1261 ctgcattata aaaacgagtt acagccccca ttgaaatcca actatcaagc aaaacattat
     1321 tttttttgaa tgaaatagta tcaaataaat catcacctgt agttgcccca tttatattat
     1381 tataaaaata ggttgacgtt ttaaaaaaca gcggttgctc ttcacttccc catactgttt
     1441 gtaatttgta tcccggcaat aaatcaacat acactctata cgttacagat ttttttgcaa
     1501 gtacaccgcc tacagtatct tttacatctt ttgattgtgc cacataatat ttctca
//
LOCUS       DAOTOW010000611         1555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_63205_length_1555_cov_2.660667, whole genome shotgun sequence.
ACCESSION   DAOTOW010000611 DAOTOW010000000
VERSION     DAOTOW010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63205_length_1555_cov_2.660667"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5243_09730"
     CDS             complement(<1..195)
                     /locus_tag="P5243_09730"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRS19772.1"
                     /translation="MARYKLTIEYDGSQYKGWQMLKNEPTIQGKLVEISSKILNTPHV
                     EVYGAGRTDAGVHALGQVAHV"
     gene            complement(182..1408)
                     /locus_tag="P5243_09735"
     CDS             complement(182..1408)
                     /locus_tag="P5243_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRS19773.1"
                     /translation="MKKILFFLFFVSVPFSLWAKQLSPTAYFSILTCAPGTQIHAHFG
                     HSAIRLCDTANNVDIVFNYGIFSYTDDFAYNFVKGETYYRLGIQQFSSFISEYYMDSR
                     GVIEQQLRLSPYQKQYLFDLLQTNYLPQNRTYLYNFFYDNCSSRPRDLLIKVLGDSLV
                     WHKPTQCIYDSLWLHTTEQYLVQEIMPSWRTFIHAYVGEESWLRFGISLGLGIPADSK
                     PTIEQTMFLPDCLFLMAQYATVKQEQSHVNLVNKTQVLVPQEVAKTQASYFSVPSHIL
                     WLICIIFIGIAGIEFWLQKHVYLVDSIVFFMFGLIGIFVWYVSFISIHPAVYPNIHVV
                     WASPILLLFAIVWLFSSLRTYTVYYLYIHIVLLLLFCLVALFQKQYIHSGYVALVIIQ
                     LSRIPFVLLQHKNGSI"
     gene            complement(1409..>1555)
                     /locus_tag="P5243_09740"
     CDS             complement(1409..>1555)
                     /locus_tag="P5243_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRS19774.1"
                     /translation="KELRGGFQAAIWQGVARMFGETLKSEYDAKGEDAMIERIIIMIE
                     QGVL"
BASE COUNT          559 a          300 c          221 g          475 t
ORIGIN      
        1 tacatgagca acttgcccta atgcatgaac cccggcatct gttctacctg caccatatac
       61 ttctacatgg ggtgtattga gtattttact actaatttca actaatttcc cctgaattgt
      121 cggctcattt ttaagcattt gccatccttt gtattgtgaa ccgtcgtatt ctattgtgag
      181 tttatatcga gccatttttg tgttgtaata atacaaaagg aattctgctt aattgaatta
      241 taactaatgc aacatatcca ctatgaatat actgtttttg gaataacgca actaaacaaa
      301 acaataaaag caatacaata tgtatataca aataataaac agtatatgtt cgcaacgaac
      361 taaaaagcca cactatagca aacaaaagaa gaatgggcga tgcccacacc acatgtatat
      421 taggatatac ggccgggtgt atacttataa aactaacata ccacacaaaa attccaatta
      481 aaccaaacat aaaaaatact atactatcta ctaaatatac atgtttttgt agccaaaatt
      541 ctatacctgc tatacctata aaaataatac atataagcca aagaatatga gacggaacag
      601 aaaaatatga tgcttgtgtt tttgcaactt cttgaggaac aagcacttgt gttttgttta
      661 ctaaatttac atgcgattgt tcttgcttta cagttgcata ttgtgccatc aaaaacaaac
      721 aatcgggtaa aaacatggtt tgctctatag ttggtttact atcggcaggt ataccaagcc
      781 ctaaacttat accaaatcgt aaccacgact cctctcctac atacgcatgt ataaaggttc
      841 gccacgaagg cattatttct tgaaccaaat actgttctgt tgtatgcaac cacaacgaat
      901 catatataca ttgtgttggt ttgtgccaca ccaaagaatc tcccaatact ttgattaata
      961 aatctctggg acgactagaa caattatcgt agaaaaaatt atataaatat gttcggttct
     1021 gtggcagata attagtttgc aataaatcga acaaatattg tttttgatac ggagacaaac
     1081 gcaattgttg ttctattaca cctcgcgaat ccatataata ttccgaaata aaactcgaaa
     1141 actgctgaat accaagcctg taatatgttt ctccttttac aaaattataa gcaaaatcat
     1201 cggtataact aaaaatacca taattaaaca ctatatcaac attgttagca gtatcacaca
     1261 aacgaatggc tgaatgtcca aaatgagcat gaatttgtgt tccgggagca catgtaagaa
     1321 ttgaaaaata tgcagtaggt gaaagttgtt ttgcccacaa tgaaaaagga acactcacaa
     1381 aaaacaaaaa aaataaaatc tttttcattt acaatacccc ttgttctatc attattataa
     1441 tacgttctat catagcatct tcaccttttg catcatattc tgatttaaga gtttcaccaa
     1501 acatgcgtgc tacaccttgc caaatagcag cttgaaaacc acctcgtaat tcctt
//
LOCUS       DAOTOW010000612         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_63436_length_1551_cov_2.322193, whole genome shotgun sequence.
ACCESSION   DAOTOW010000612 DAOTOW010000000
VERSION     DAOTOW010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63436_length_1551_cov_2.322193"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="P5243_09745"
     CDS             <1..968
                     /locus_tag="P5243_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19775.1"
                     /translation="ENKFIGLKGADLMLLNPAKYEEAFNMCKKSVDALGNKSEVKTLV
                     MYMQANVSKFENNTCTKAEALQVYQQIASIFDANIQKGSQAHEKQLPVIENMFLQLKP
                     ECTDLVALYEPQYKANPQNVDVLKKIISNLGKTCKDSDLYLQAVVSLDKIEPSALSKR
                     SIAEMYVDKKQNNTAINYFKESIDLETDNNQKAETYYRMASITMGSASTSVAYAQSAL
                     KLNPNMGKAHLFIAAQYVNSINSCIDKAEYPDVAKWTILWAAVDMCNKAKSVDPSVTS
                     QANSQIANYRAHFPDAEALFGYNITEGAPMSFSCWFTVNTTAKVK"
     gene            972..1541
                     /gene="lptC"
                     /locus_tag="P5243_09750"
     CDS             972..1541
                     /gene="lptC"
                     /locus_tag="P5243_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRS19776.1"
                     /translation="MITNIFSYSSSIVILSIAMLLVSCKNDIKNVIKVQEVEKLPELL
                     GEEIYLRQSEMGEINISVFAKKFVKYNSQNKKLTEFPEGIIVVHYSQYPDTLSKISAN
                     YAVNYDDKEIWEAKGNVVAKNKKNEILNTEYLVWDQKKKIIFSDKQVQVTTPNDIIFG
                     EGFESDEQFENWKIQKVTGIFTLDDNTSE"
BASE COUNT          640 a          229 c          244 g          438 t
ORIGIN      
        1 aagaaaataa atttattgga ttaaaaggag cagatttaat gcttttaaat cctgctaaat
       61 atgaagaagc ttttaatatg tgtaaaaaat cggttgatgc attgggaaac aaatccgaag
      121 taaaaacact cgtaatgtat atgcaagcta atgtatcaaa atttgaaaat aatacatgta
      181 caaaagccga agcattacaa gtatatcaac aaatagcttc tatatttgat gccaacatac
      241 aaaaaggctc tcaagcacac gaaaaacaac tgcccgttat tgaaaatatg ttcttacaac
      301 ttaaacccga atgtactgat ttagtagcct tgtatgagcc acaatacaaa gccaatccgc
      361 aaaatgtaga tgtattgaaa aaaataattt caaatttagg aaaaacttgt aaagacagtg
      421 atttatatct acaagctgtt gtaagcttag acaaaattga accttcagca ttatcaaaaa
      481 gaagtattgc cgaaatgtat gttgataaaa aacaaaacaa taccgcaatc aattatttta
      541 aagaatcaat tgatttagaa accgataaca atcaaaaagc tgaaacatac tatagaatgg
      601 catcaattac aatgggtagt gcaagcacta gtgtagcata cgcacaaagc gccctaaaat
      661 taaatcctaa tatgggtaaa gcacacttat ttattgctgc tcaatatgtt aacagtatca
      721 attcttgtat tgacaaagca gaatatcctg atgtagcaaa atggaccata ctatgggctg
      781 ctgtagatat gtgtaataaa gcaaaatcag ttgacccatc agttacaagt caagctaata
      841 gccaaatagc taattacaga gctcacttcc ctgatgccga agctttattt ggctataata
      901 taacagaggg tgctccaatg tcattttcgt gttggtttac ggtaaatact acagcaaagg
      961 ttaaataacc catgattaca aacatttttt catattcaag cagcatagta atacttagta
     1021 ttgcaatgct gcttgtttca tgtaaaaacg atataaaaaa tgtaataaaa gtacaagaag
     1081 ttgaaaaact tcctgaatta ttaggcgaag aaatttattt acgtcaaagt gaaatgggcg
     1141 aaataaatat aagtgtattt gccaaaaaat ttgtaaaata caattcccaa aacaaaaaat
     1201 taacagaatt tcctgaagga attattgtag ttcattattc tcaatatcct gacacgttat
     1261 caaaaatttc tgcaaattac gcagtcaatt atgatgataa agaaatttgg gaagcaaaag
     1321 gaaatgtagt agcaaaaaac aaaaaaaatg aaatactcaa caccgagtat ttagtgtggg
     1381 atcaaaaaaa gaaaataatt ttttctgata aacaagtaca agtaactaca ccaaatgata
     1441 ttatatttgg tgaaggattt gaatctgatg aacaatttga aaattggaaa attcaaaaag
     1501 ttaccggtat atttactctt gatgataaca caagtgaata atatgacaaa a
//
LOCUS       DAOTOW010000613         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_63440_length_1551_cov_2.294118, whole genome shotgun sequence.
ACCESSION   DAOTOW010000613 DAOTOW010000000
VERSION     DAOTOW010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63440_length_1551_cov_2.294118"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1551)
                     /gene="gldG"
                     /locus_tag="P5243_09755"
     CDS             complement(<1..>1551)
                     /gene="gldG"
                     /locus_tag="P5243_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRS19777.1"
                     /translation="FMVLLQRIKHIAIATAVLIIVLWISSIQYGRIDVTGDKRYTLNP
                     STVTFLEELQDTIRFSVYLHGELPLAFAKMKREVNDMLNEYKRIGGAHVQILFIDPAS
                     LSKGKNSIQNTYKRLMQYGLKPYTIQEEDEQGKLVQRYILPGIIVSTSQKYVPINLLA
                     NALGNSTEEQIYNALQQLEYQCIKSMKQLTAKKRKNIAFLTDHKELDFQYVFDATMLL
                     MEQYNVDRISTQQLFDSITKYDVVILAKPRTALSEAHKYILDQFVMHGGNLLIATDNV
                     GVNIDSLQIRSHTTAYPLQLNSSDLFFNFGFRINNTIVLDNQCATIPFNISPLGMPPR
                     FEPAPWYYYPLLKPLRNSHHPITHAIDVVKAEFASSLDTLEGNGTIQKHILLASSAYS
                     RTIGVPNPVGFSILDNVPDKTFFNTYYLPIAVLFEGSYNSLYKNRTAPIDTELIPKQF
                     SPLYSSDSTKIIVISDGDIFKNEIEIRENDTIPKPMHYYKYFAVDKRIYTGNKDFFLN
                     SVNYLCGD"
BASE COUNT          486 a          233 c          264 g          568 t
ORIGIN      
        1 gatcaccgca taaataattt accgaattca aaaagaaatc tttatttccg gtataaatac
       61 gtttatcaac tgcaaaatat ttgtaataat gcattggctt aggtattgta tcattttcgc
      121 gtatttcaat ttcatttttg aaaatatcac catctgaaat gactattatt ttagtactat
      181 ctgatgaata gagtggcgaa aattgttttg gaataagttc tgtatcaata ggtgcagttc
      241 tatttttata caatgaatta taagaacctt caaataatac agctataggt aaataatacg
      301 tgttaaaaaa tgttttgtca ggaacattat ctaaaatgct aaatcctaca gggttaggta
      361 ctcctatggt gcgtgaatat gccgatgatg ccaaaagaat atgtttttgt atggttccat
      421 tcccttctaa tgtgtcaagt gacgaggcaa attctgcttt taccacatca atagcatgtg
      481 ttattggatg gtgagagttt ctaagaggtt taagcagagg ataataatac catggtgcag
      541 gttcaaaacg aggaggcatt cctaaaggac taatattaaa tggaattgtt gcacattgat
      601 tgtctaatac aatagtattg tttattctaa atccaaaatt gaaaaataaa tcggaactat
      661 tgagttgtaa tggataggct gtagtatgcg aacgaatttg taacgaatca atatttactc
      721 caacattgtc ggttgctata agtaaattgc caccgtgcat tacaaattgg tcaagtatat
      781 atttatgagc ttcgctcaat gctgtacgtg gtttagcaag tattaccaca tcgtattttg
      841 ttatggaatc aaataattgt tgtgtcgaaa ttctgtcaac attatattgc tccattagta
      901 gcatggtagc atcaaataca tattgaaaat ctaattcctt atgatctgtt aaaaaagcaa
      961 tgtttttacg ctttttggct gttaattgct tcatgctttt tatgcattga tactctaact
     1021 gttgcaatgc attgtatatt tgttcttcgg ttgaatttcc taatgcattg gcaagtaaat
     1081 ttattggcac atatttttgt gatgtagata ctataatgcc cggtaatata tatcgttgta
     1141 ccaatttccc ttgttcatct tcctcttgaa ttgtatatgg ttttaaccca tattgcatca
     1201 atcgtttgta tgtgttttgt attgaatttt ttcctttact caatgatgca gggtcaataa
     1261 ataatatttg tacatgggct ccacctatgc gtttgtattc attaagcata tcatttactt
     1321 cgcgcttcat ttttgcaaac gccagcggta attctccatg taaatacaca gaaaaacgaa
     1381 ttgtatcttg taattcttct aaaaatgtaa cagtagatgg gtttaaagta tagcgtttgt
     1441 ctccggtaac atctattctg ccatattgaa ttgaagaaat ccaaagtact ataataagaa
     1501 ctgctgtagc tattgcaata tgttttattc gttgtaataa taccataaat t
//
LOCUS       DAOTOW010000614         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_64083_length_1540_cov_4.422896, whole genome shotgun sequence.
ACCESSION   DAOTOW010000614 DAOTOW010000000
VERSION     DAOTOW010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64083_length_1540_cov_4.422896"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="P5243_09760"
     CDS             <1..739
                     /locus_tag="P5243_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRS19778.1"
                     /translation="LTLKVWRQKGPKEAGRFETYSVSNISPDSSFLEMMDVLNEQLIV
                     EGKDPVAFDHDCREGICGMCSLYINGHAHGPDEDVTTCQLHMRKFKDGETVTIEPWRS
                     AGFPVIKDLIVDRSAFDKIMAAGGFISVNTGGVPDGNAIPIPKKDADEAMDAASCIGC
                     GACVATCKNGSAMLFVAAKVSQLALLPQGRVEAARRAKSMVAKMDELGFGNCTNTGAC
                     EVECPKGISIANIARLNREFLLAKLQD"
     gene            complement(805..>1540)
                     /locus_tag="P5243_09765"
     CDS             complement(805..>1540)
                     /locus_tag="P5243_09765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS19779.1"
                     /translation="VNDAHYLPFRKYILTKSNGIFFISRRGYDYYAELFPLLVQKMRV
                     SYLGVIGYFQIKTTRPNVCRIVSCSNVISIKQIDLIIHALTQIREYQIEWIHFGDGEL
                     FQQIQKLTHELLDKKTNISFSFAGRVSNTEVMNYYKNNYVDCLINVSLSEGIPVSMME
                     AMSFGIPVIGTNVGGVSEIIEHETNGFLLSPTPSKEEVAAAIERFCSLTHDEVQTMRN
                     NAYNTWDTKFNAQKNYKEFIRLIQSL"
BASE COUNT          513 a          280 c          275 g          472 t
ORIGIN      
        1 cctcacactt aaagtgtggc gccaaaaagg accaaaagaa gcaggacgat ttgaaacgta
       61 ttctgtatcg aatatttcac ccgacagttc gttcctcgaa atgatggacg tgcttaacga
      121 acaattaata gtagaaggca aagaccctgt tgcattcgac cacgattgtc gcgaaggtat
      181 ttgcggtatg tgtagtttat acatcaatgg tcacgcacac ggaccagacg aagatgtaac
      241 tacttgccaa ttacacatgc gtaaatttaa agatggcgaa acagtaacaa tagaaccatg
      301 gcgttctgca ggtttccctg taataaaaga cttaattgta gaccgtagtg catttgataa
      361 aataatggct gccggtggat ttatttcggt aaataccggt ggtgtacccg atggtaatgc
      421 tatacctatt cctaaaaaag atgcagatga agctatggat gcagcatctt gcattggctg
      481 tggagcatgt gttgcaacat gtaaaaacgg atcggcaatg ttatttgtag ctgccaaagt
      541 atcacaattg gctttattac ctcaaggtag agtagaagct gcacgacgag ctaaatctat
      601 ggttgcaaaa atggacgaac ttggctttgg aaactgtaca aataccgggg cttgcgaagt
      661 ggaatgtcca aaaggtattt ctattgcaaa tattgctcgt ttaaaccgcg aatttttatt
      721 ggcaaaatta caagattaaa caaacataat tacactttaa aagcccctgt tgaaatctat
      781 ctacaggggc ttttttatgc tatattataa tgattgtatc aaacgaataa actctttata
      841 attcttttga gcattaaatt ttgtatccca agtattatat gcattgttac gcatagtttg
      901 cacctcatcg tgtgtcaatg aacaaaaccg ttcaatagca gcagctactt cttctttgct
      961 tggagttggc gaaagtaaaa aaccatttgt ttcgtgttca ataatttcgc tcacgccacc
     1021 cacattggta ccaataacag gaataccaaa actcatggct tccatcatgg atacggggat
     1081 gccttctgaa agacttacgt ttattaaaca atcaacataa ttgtttttat aataattcat
     1141 aacttctgta ttagacaccc gacctgcaaa agaaaaagaa atatttgtct ttttatctaa
     1201 taattcatgt gttaatttct gaatttgttg aaataattca ccatcgccaa aatgaatcca
     1261 ctctatttga tattcacgga tttgcgttaa tgcatgtata attaaatcaa tttgttttat
     1321 agatattaca tttgaacaac ttacaattct acacacattg ggtcgtgtgg tttttatttg
     1381 aaaataacct ataacaccta aatatgaaac tctcattttt tgaacaagca acggaaataa
     1441 ttcagcataa taatcataac ccctccgaga aataaaaaag ataccattag attttgtcaa
     1501 aatatatttt cgaaaaggca aatagtgcgc atcattcact
//
LOCUS       DAOTOW010000615         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_64114_length_1540_cov_2.115152, whole genome shotgun sequence.
ACCESSION   DAOTOW010000615 DAOTOW010000000
VERSION     DAOTOW010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64114_length_1540_cov_2.115152"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="rfbD"
                     /locus_tag="P5243_09770"
     CDS             complement(<1..594)
                     /gene="rfbD"
                     /locus_tag="P5243_09770"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRS19780.1"
                     /translation="MATILVTGANGQLGNELQILAKQSKYTWLFTDIAELDICNKDAI
                     ESFVISNNCTYIINCAAYTAVDKAETDTEIAYAINATAPKNIAEVCAQHKCFFIHIST
                     DYVFDGTKHTPYIETDTTNPQSIYGASKLRGENNALASNQNTCIIRTSWLYSTYGNNF
                     VKTMIRLGNERDQLNVIYDQIGTPTYARDLAAAIIHIV"
     gene            complement(598..1149)
                     /gene="rfbC"
                     /locus_tag="P5243_09775"
     CDS             complement(598..1149)
                     /gene="rfbC"
                     /locus_tag="P5243_09775"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRS19781.1"
                     /translation="MNITPTHIDGVYIIEPRVFSDARGHFFESYNKLRLEEKGIEYTF
                     VQDNQSKSSYGVIRGLHFQKPPHAQTKLLRVVEGIIIDVAVDIRKNSPTYGQHVAIEL
                     SADNFKQLLIPEGFAHGFSVISETAIVQYKCTDYYYPETEGGISYNDSSLGINWQIPL
                     VKAIVSEKDAKLPTLQDFITPFI"
     gene            complement(1149..>1540)
                     /locus_tag="P5243_09780"
     CDS             complement(1149..>1540)
                     /locus_tag="P5243_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS19782.1"
                     /translation="PKSNYAVTGLYFYDNSVVQKAKNLKPSTRGELEITDLNKLFLHE
                     GTLQVKLLGRGIAWLDTGTYDSLLEASNYIAAIENRQGLMVANLEEIAYVKGYISKQQ
                     LLDLAEPLKKNSYGQYLIKIANNKVLE"
BASE COUNT          460 a          254 c          261 g          565 t
ORIGIN      
        1 aactatgtga attattgctg cagccaaatc gcgagcataa gtaggtgttc ctatttgatc
       61 ataaattaca tttaattggt cgcgttcgtt acctaaacga atcatggttt ttacaaaatt
      121 atttccatac gttgagtata accatgatgt gcgaatgata cacgtatttt gattagatgc
      181 gagagcatta ttttcaccac gcaatttgct tgcaccgtaa atagattgcg gattagttgt
      241 gtctgtttca atatagggag tatgctttgt gccatcaaaa acatagtcgg tagaaatgtg
      301 aataaaaaaa catttgtgtt gagcacatac ttctgctata ttttttggag ctgttgcatt
      361 tattgcataa gctatttctg tatctgtttc tgctttgtca acagcagtat atgcagcaca
      421 gttaatgata taggtgcagt tatttgaaat tacaaatgat tcaatagcat ctttgttgca
      481 tatatcaagt tcagctatat ctgtaaacag ccaagtatat tttgattgtt ttgccaatat
      541 ttgtaattca ttaccaagtt gaccgttagc accggttaca agaatagtag ccatagttta
      601 aataaatgga gttataaaat cttgtaatgt tggaagtttt gcatcttttt cagatactat
      661 tgctttgact aatggaatct gccaattaat acctaaacta ctgtcattat atgatatacc
      721 accttctgtt tcgggataat aatagtctgt gcacttgtat tgcactattg cggtttcact
      781 tatcaccgaa aaaccatgag caaagccttc cggtatgagt aattgtttga aattgtcagc
      841 acttaattca atagccacat gctgcccata tgtaggtgaa ttttttctga tatcaacagc
      901 aacatctata attatgcctt ctacaactcg taatagtttt gtctgtgcat gaggaggctt
      961 ttgaaaatgt aagcctcgta tcacaccata acttgatttt gattgattat cttgcacaaa
     1021 ggtgtattct ataccttttt cttctaatcg taatttattg taactttcga aaaaatgacc
     1081 tcgagcatca gaaaatacac gtggttcaat gatgtataca ccgtcaatat gagtaggagt
     1141 aatattcatt attctagtac tttattgttt gcaattttaa taagatattg tccgtaacta
     1201 ttttttttga gtggttcagc gagatctaat aattgttgtt ttgatatata gccttttaca
     1261 tatgctattt cttcaagatt tgcaaccata aggccttgac gattttcaat agctgcaata
     1321 tagtttgaag cttctaataa actgtcatac gtaccggtat cgagccatgc aatgcctcgt
     1381 cccaataatt ttacttgtag agtaccttca tgtaaaaaca atttatttaa gtcagttatt
     1441 tctaactcac ctcgagtcga tggtttaaga ttttttgctt tttgaactac cgaattgtca
     1501 taaaaatata atcctgtaac tgcatagtta gattttggtt
//
LOCUS       DAOTOW010000616         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_64178_length_1539_cov_2.441375, whole genome shotgun sequence.
ACCESSION   DAOTOW010000616 DAOTOW010000000
VERSION     DAOTOW010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64178_length_1539_cov_2.441375"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1109
                     /locus_tag="P5243_09785"
     CDS             234..1109
                     /locus_tag="P5243_09785"
                     /inference="COORDINATES: protein motif:HMM:NF039711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HRS19783.1"
                     /translation="MEILYSCNCKTITHHQIGVVLKQISDLLHSKGFSQYIVRKVYSI
                     SSECLDNILQHGYNDIQNSKPPHFEIKSDNHSIYIYASNSVKKNDLEKLQHTISIMNE
                     MRYVDLKPHFQNTIKEKSMLTSGGAGVGLIMIKRKSELPIELEVETLTKDIAYVTFKI
                     ELEIGTMKKFKKLATKHTPHINFSILSGIFSIQGISRPENADAYYQEIIQWVEEHTDE
                     IQQMQSLKLHVELEYINSVSLKNMLRLLRTIISINPHNTIVEWVYDKEDETSKEEGEE
                     LSEILKKEFVFIEKK"
     gene            1172..>1539
                     /locus_tag="P5243_09790"
     CDS             1172..>1539
                     /locus_tag="P5243_09790"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HRS19784.1"
                     /translation="MKNIEIEGSPKTPEINFNAETGVIEIKGRSIPENSIEFYKPMID
                     WLEEYATAPKPKTIVNIQLEYFNTSSSKCILDVFKKLEVISKNGNDVQVNWYYEEDDE
                     DMLEAGEDYQSIIRIPFKMI"
BASE COUNT          604 a          177 c          235 g          523 t
ORIGIN      
        1 ctgcttgcgc cgatggtact gcgttttgtg ggagagtagg tcgccgccga tttttttaag
       61 cccgatacag aaatgtgtcg ggcttttttg tttgtaacaa ctttgaataa tgcaattttt
      121 attgtatttg ttgttttatc tgaatttaaa aatatctcat atttattttt actatataat
      181 catatgtttg taaaaaaaaa tgtatatttt tacctaccat tttaagtata tgtatggaaa
      241 tactttattc ttgtaactgc aaaactataa cacatcatca aataggagtg gtattgaaac
      301 aaatttctga tttgttacat tcgaaaggtt tttcgcagta tattgttcga aaagtatatt
      361 ctatttcatc agaatgttta gataatattt tgcaacacgg atataacgat atacaaaatt
      421 ctaagcctcc gcattttgaa ataaaatcag ataatcacag tatatatata tatgcttcaa
      481 attctgttaa aaaaaatgat ttagaaaaat tacaacatac tatttcaata atgaatgaaa
      541 tgaggtatgt tgatttaaaa ccacattttc agaatactat taaagagaaa agtatgctta
      601 cttcaggagg tgccggtgtt ggactcataa tgataaaacg taaatcagaa ttacctattg
      661 aattagaagt agaaacgctt acaaaagata ttgcatatgt tacatttaaa attgaattag
      721 agataggaac aatgaagaaa tttaaaaaat tagcaacaaa acatactcct catataaatt
      781 ttagtatttt atcaggtata ttttcaatac aaggaatatc tcgtcccgaa aatgctgatg
      841 catattatca agaaattata caatgggttg aagaacatac tgatgaaata caacaaatgc
      901 aaagtctcaa actgcatgta gaattagagt atataaattc tgtttcactt aaaaatatgt
      961 tacggttatt aagaactatt atttcaataa atcctcacaa tacaatagta gaatgggtgt
     1021 atgacaaaga agatgaaaca tcaaaagaag agggagagga attaagtgaa attctaaaaa
     1081 aagaatttgt atttattgaa aaaaaataaa agatatttat ttttaattcg taaaattgta
     1141 catttgtaaa tatatttaaa aactcaaata catgaaaaat atagaaattg aaggttctcc
     1201 aaaaactcct gaaattaatt ttaatgcaga gaccggtgtg attgaaatta aaggtagatc
     1261 tattcctgaa aattcaattg aattttataa acctatgatt gattggttag aagagtacgc
     1321 tacagctcct aaacctaaaa ctattgttaa tattcaatta gaatatttta atactagttc
     1381 atctaaatgt attttagatg tatttaaaaa attagaagta atttctaaaa atggaaatga
     1441 tgtacaggta aattggtatt atgaagaaga tgatgaagat atgcttgagg caggtgaaga
     1501 ttatcaatct attattcgta taccttttaa aatgataga
//
LOCUS       DAOTOW010000617         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_64292_length_1537_cov_3.841430, whole genome shotgun sequence.
ACCESSION   DAOTOW010000617 DAOTOW010000000
VERSION     DAOTOW010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64292_length_1537_cov_3.841430"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="P5243_09795"
     CDS             complement(<1..376)
                     /locus_tag="P5243_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRS19785.1"
                     /translation="MKKNFRKRSTSELERKNIHEYKNAVKTPIVLVLDNIRSANNVGS
                     IFRTADAFLIEHIYLCGITACPPHKDIHKTALGAELSVPHTYYNSTTEAINFLQSHNY
                     TIISIEQAEGSTALHNFEQTTGE"
     gene            complement(360..758)
                     /locus_tag="P5243_09800"
     CDS             complement(360..758)
                     /locus_tag="P5243_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19786.1"
                     /translation="MKYLLIILFICCLQHNSNAQIQGNSASIPEQTNSTDPLSTITVS
                     VYWVQDSLPYTISCYRVQGKDAIMLKTSKFRGYTIDFHRNMAKSMFKQTLESPETEIS
                     IKTIFEGKYYLDVIDPQGIKVKTFLIEKKF"
     gene            942..1514
                     /locus_tag="P5243_09805"
     CDS             942..1514
                     /locus_tag="P5243_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19787.1"
                     /translation="MKLSRIILLFGLLLFVGNAAFAQYDDDDGGSGRKASSKKEKVKA
                     PKAQKSGAKQSKTREDLFRSSVNKTTYLSKKEQQKLIAKKERKKEKHYKKTRGEVNNK
                     STVKRMKKSERHSYKVNHNIQGSFLKRVSYSINPETAKARRKSSSQNRRLQKNQEKAR
                     DKYENTEYGKNTKKKKKNKKLRSFYNPLSN"
BASE COUNT          547 a          205 c          243 g          542 t
ORIGIN      
        1 tttcacctgt tgtttgttca aaattgtgca aagccgtact gccttcggct tgttctatac
       61 ttataatagt ataattgtgt gattgtaaaa aattaattgc ttctgtggtt gaattataat
      121 aggtgtgggg caccgaaagt tcagcaccta atgctgtttt gtgaatatct ttgtgcggag
      181 gacaagctgt tattccgcat aaatatatat gttcaattaa aaatgcatct gcggttctaa
      241 atatagaacc tacattgttt gcgcttctaa tattatcaag caccaataca ataggtgttt
      301 ttactgcatt tttatattcg tgaatatttt ttcgttctaa ctcagaagta ctgcgttttc
      361 taaaattttt tttcaatcaa aaatgttttt acttttatac cttgtgggtc aattacatct
      421 aaatagtatt taccttcaaa aattgttttt atacttattt cggtttcggg gctttctaaa
      481 gtttgtttaa acatactttt agccatgttt ctatgaaagt ctatcgtgta tcctctaaat
      541 tttgatgttt tcaacattat tgcatctttg ccttgaacac gatagcagga aatggtatat
      601 ggtaaactat cttgaaccca atacacactc actgtaatag ttgataaagg atcggtactg
      661 tttgtttgtt cgggaatgct tgcagaatta ccttgaattt gagcgttgct attgtgttgt
      721 aaacaacata taaacagtat tattaacaaa tatttcatat atgtaatttt ttttgtaaat
      781 aaatgactta aaataagtta aaaagatatt ttcgtgtaat tgttgttata ttttcaaaac
      841 ttataaacac ccgtaaaaat ataaaaactt tttaattact tttgctttca agttttaaaa
      901 aaatgttttt ctaattaagt ttgagtattt ttaactaacg catgaaattg tctcgtatta
      961 ttttactttt tggtttgttg ttgtttgttg gtaatgctgc ctttgctcaa tacgatgatg
     1021 atgatggcgg ttcaggtaga aaagcttcgt caaaaaagga aaaagtaaaa gctcctaaag
     1081 cacaaaaatc aggtgcgaaa caatcaaaaa cacgtgaaga tttatttaga agtagtgtaa
     1141 ataaaactac atatttaagt aaaaaagaac aacaaaaact tattgcaaaa aaagaacgga
     1201 aaaaagaaaa acattataaa aaaactcgtg gtgaagtaaa taataagagt actgtaaaac
     1261 gaatgaaaaa atctgaacgt cattcgtata aagtaaatca taatattcaa gggtcatttt
     1321 taaaacgagt ttcatattca ataaatccgg aaacagccaa ggcacggcgt aaatcatcga
     1381 gtcaaaatag aagactgcag aaaaatcagg aaaaagctcg tgataagtac gaaaatactg
     1441 aatatggtaa aaatactaaa aagaagaaaa agaataaaaa attacgttct ttttacaatc
     1501 cgttatcaaa ttaatttgct ttattttgta taagttt
//
LOCUS       DAOTOW010000618         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_64666_length_1531_cov_2.865176, whole genome shotgun sequence.
ACCESSION   DAOTOW010000618 DAOTOW010000000
VERSION     DAOTOW010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64666_length_1531_cov_2.865176"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1165
                     /locus_tag="P5243_09810"
     CDS             8..1165
                     /locus_tag="P5243_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HRS19788.1"
                     /translation="MYSQKFEGLAKTPPMGWNSWNTFETNINEDLIIDMVDSIISKGL
                     KDAGYTYIVLDDGWMARERDAQGNLIADPVKFPKGMKHLADYVHSKGMKFGLYNCAGK
                     LTCAGYPGSRGHEYQDARKYAEWGVDFLKYDWCYTDKLNAEEAYITMRDALYATGRPI
                     ILSICEWGNNEPWKWGSKVGHLWRVTGDITNCFDCEVNHGTWSSWGVMKIVYLRDSIR
                     QYTKPDAWNDYDMMEVGNGMTAGEDRAHFALWSIFASPLIMGNDVRNASKETIEILSN
                     KEVIAINQDPLGIHGFKYMEQDSLEVWVKPLKNDAWALCFLNRNTHPYSLNFDWNTHS
                     IHDKIFNKKLQFSQGVYSIRNVYTKKDIGTTKKALQTTIPAHDVLFVVVKK"
     gene            1244..>1531
                     /locus_tag="P5243_09815"
     CDS             1244..>1531
                     /locus_tag="P5243_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19789.1"
                     /translation="MKKYILFAILCLITISSFAQAPGAPVVDINSGNPKFPFPQFLPY
                     SYGTNHTLGNLGTNNAPGVVHAEMEKLIREAWQIFANEFTYTGQTFGGVQYI"
BASE COUNT          530 a          271 c          309 g          421 t
ORIGIN      
        1 aataaacgtg tattctcaaa aatttgaagg attagcaaaa actcctccta tgggttggaa
       61 cagctggaat acatttgaaa ctaatataaa cgaagatctt atcattgaca tggttgacag
      121 cataatttca aaaggattga aagatgccgg atacacatac atagtattag acgatgggtg
      181 gatggctcgc gaacgtgatg cacaagggaa tttaatagcc gaccccgtaa aattccccaa
      241 aggcatgaaa catcttgccg attatgtaca ttccaaaggg atgaaatttg gcttatacaa
      301 ttgtgccgga aaacttacct gtgcaggata tccgggcagt agaggtcatg aatatcaaga
      361 tgctcgaaaa tatgcagaat ggggagttga ttttttgaaa tatgattggt gctacaccga
      421 taaactcaat gccgaagaag cttacataac tatgcgcgat gccttgtatg ctacaggcag
      481 accaataata ttgagcattt gcgaatgggg aaacaacgaa ccatggaaat ggggcagtaa
      541 ggtaggccat ttatggcgag taaccggaga tataacgaac tgttttgact gtgaggtaaa
      601 ccatggcaca tggtcgagtt ggggagttat gaaaattgta tatctgcgcg acagtattcg
      661 ccaatatacc aaacccgatg cgtggaacga ctacgacatg atggaagttg gcaacggtat
      721 gaccgctggc gaagaccgtg cacattttgc actgtggagc atatttgcat cgccgcttat
      781 aatgggtaac gatgtgcgaa atgcaagcaa agaaaccatt gaaattttat ctaataaaga
      841 agtaattgca ataaaccaag accctctggg aatacatggt ttcaaatata tggaacaaga
      901 cagtttagaa gtatgggtaa aaccacttaa aaatgatgca tgggcactat gctttctcaa
      961 cagaaacact cacccgtatt cgcttaattt tgactggaat acacacagta ttcacgataa
     1021 aatttttaat aaaaaattac aattttcaca aggggtttat tctatacgaa atgtctatac
     1081 aaagaaagat attgggacta caaaaaaagc attacaaaca acaataccgg ctcatgacgt
     1141 attgtttgtg gttgttaaaa aataatcaat cataaatgca accttttttg cattttttta
     1201 gtctttggta tcctatctaa atactatatt aaaacaaata tatatgaaga aatatatact
     1261 ttttgcaata ctatgtttga taacaatatc atcgtttgca caagcaccgg gagctcctgt
     1321 ggttgacatc aattccggta atcctaaatt tccgtttcca caatttttac catattcata
     1381 cggaactaat cacactttag gtaatttagg taccaacaat gcccccggag tagtacatgc
     1441 cgaaatggaa aaattaatac gtgaagcatg gcaaatattt gctaatgaat tcacttatac
     1501 cggccaaaca tttgggggcg ttcaatacat t
//
LOCUS       DAOTOW010000619         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_64795_length_1529_cov_2.440977, whole genome shotgun sequence.
ACCESSION   DAOTOW010000619 DAOTOW010000000
VERSION     DAOTOW010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64795_length_1529_cov_2.440977"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1002
                     /locus_tag="P5243_09820"
     CDS             58..1002
                     /locus_tag="P5243_09820"
                     /inference="COORDINATES: protein motif:HMM:NF006263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRS19790.1"
                     /translation="MNSIVVLDAQTVGNNTVFKRFSDFGSLTIYGTTTKEERIAHIGN
                     ASIIITNKVIIDAFIMDSCPQIQLICLTATGMNNVDLEYASKKGIVVKNVAGYSTESV
                     AQHTFAMFLSLLHSIEYYNSYVKNKEYSHQQLFTHIGPGYWQLQDKTWGIIGLGTIGK
                     RVAQIAQVFGAHIIYYSTSGKNYNSQYTQVSLQELLIKSDVVSIHAPLNEQTLGLIGI
                     DELQHMKSHAYIINVGRGGIVNENDLAEALQKNIIAGACLDVMQQEPIDETSPLLDPK
                     IAHKIIITPHVAWISKEALQTLIECVYNHVKEFVKKEL"
     gene            complement(997..>1529)
                     /locus_tag="P5243_09825"
     CDS             complement(997..>1529)
                     /locus_tag="P5243_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRS19791.1"
                     /translation="ILGIIRPCTEQAGSFTANNNLGIFATNGTVSSHSYEIEIQKFFP
                     HITVTSHACPMWVPLVENGESQSEGANYFIQKEINAILTKNPSIDSILLACTHYPILL
                     PKIREFLPKHISIINQGNIVAHSLQKYLQNHPEIEHKILKNSNTQYFTTENADVFNSI
                     AECFTFNKIQARTITL"
BASE COUNT          567 a          190 c          262 g          510 t
ORIGIN      
        1 ttgaaacaaa tgaattacgc tggcttgaat tacaagaaaa attataacat gaaaaatatg
       61 aattcaatag ttgtattaga tgctcaaaca gttggtaata atactgtatt taaaagattt
      121 agtgatttcg gaagtcttac catatatgga acaacaacta aagaggagcg aatagctcat
      181 attggtaatg catcaattat aattacaaat aaagtaataa tagatgcttt tattatggat
      241 tcttgtccac aaatacaact aatttgttta acggctacgg gcatgaacaa tgttgattta
      301 gaatatgcat caaaaaaagg tatagtggtt aaaaatgttg caggatattc tacagaaagt
      361 gttgcacaac atacatttgc aatgttttta agtttattgc attctattga atattataat
      421 tcttatgtta aaaataaaga atattcgcat caacaattgt ttactcatat tggtccagga
      481 tattggcaat tacaagataa aacatggggt attataggat taggtactat tggtaaacgt
      541 gttgcacaaa ttgctcaagt atttggggca catataatat attattctac atcaggtaaa
      601 aattataatt ctcaatatac tcaagtatca ttacaagaat tattgataaa gtctgatgtt
      661 gtgagtattc atgctcctct taacgaacaa acattgggac taataggaat agatgagttg
      721 caacatatga aatctcatgc atatataatt aatgtaggca gaggtggtat tgtaaatgaa
      781 aatgatttag ctgaagcttt gcaaaaaaat ataattgcag gagcatgttt agatgttatg
      841 caacaagaac caatagatga aacatcaccg ttgcttgatc ctaaaatagc tcataaaata
      901 attataacac cccacgttgc atggataagt aaagaagctt tacaaacctt aattgaatgt
      961 gtgtacaatc atgtaaaaga atttgtaaaa aaagagttat agtgttattg tacgagcttg
     1021 tattttattg aaagtaaagc attctgcaat tgaattaaat acatcagcat tttctgtagt
     1081 aaaatattgt gtattggaat tttttagaat tttatgttca atttcaggat gattttgtaa
     1141 atatttttgt aaactatgcg ctacaatatt tccttgatta ataatagaaa tatgttttgg
     1201 taaaaattct ctaattttag gcaataaaat agggtagtgg gtgcaagcta gaagtataga
     1261 atcaatagaa gggtttttgg taagtatagc attaatttct ttttgtataa agtaattagc
     1321 accctctgac tgagactctc cattttcaac taatggaacc cacataggac aagcatgaga
     1381 tgtaactgta atgtgaggaa aaaatttttg aatttcaatt tcatatgaat gcgaactaac
     1441 agttccgtta gtagcaaaaa ttcctaaatt attattcgca gtaaaagagc ctgcttgttc
     1501 ggtacaaggc ctaattatgc ctaatatgc
//
LOCUS       DAOTOW010000620         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_64849_length_1528_cov_3.003394, whole genome shotgun sequence.
ACCESSION   DAOTOW010000620 DAOTOW010000000
VERSION     DAOTOW010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64849_length_1528_cov_3.003394"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="P5243_09830"
     CDS             complement(<1..399)
                     /locus_tag="P5243_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRS19792.1"
                     /translation="MGRKKNFPILEHVQIIDIAAEGKAIAKVDDLIIFVEGAIPNDIV
                     TLQITKKKKRYCEARILTFEKYSSQRTEAFCKHFGICGGCKWQILPYHLQLEYKEKQV
                     TDQLTRIGHVHIQQSLPIVGADETTLYRNKL"
     gene            461..1441
                     /locus_tag="P5243_09835"
     CDS             461..1441
                     /locus_tag="P5243_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRS19793.1"
                     /translation="MEIFTHLLLGAIVAGLFGARYIGNWAFLWGALLSLIPSIDMLIL
                     IFSDSLEGLNFLRGITHSILFILVMSPLAAILLSKITFHSTITIAQWTRLILLVFASH
                     VAYDIFTIQGVGLLEPFTDKRYALCSIADFDLFFVIPLLAALALALKVSQNRHKTIIV
                     WFGIFISVLYISFTFLNKLYIQSEFEKKLVAEKIRFDRTEIFPVLGSNFLWNCIAQDR
                     DGFWMCYETNFSKHNFEMNLYMRNDYYIFDFENDSRIQKIKSSTKDYYIVQKMGNGDV
                     FIYDLRMGRMGLNSQAPFMYSYRIKNMHGTIQSLEKIEPSFYSIFFKKSK"
BASE COUNT          478 a          201 c          246 g          603 t
ORIGIN      
        1 taatttattg cgatataatg tagtttcatc agctccaact attggaagag attgttgaat
       61 atgaacatga ccaatgcgtg taagttgatc tgtaacttgt ttttctttgt attctaattg
      121 taaatgatag ggaagtattt gccatttgca cccgccacat attccgaaat gcttacaaaa
      181 agcttctgtt ctttgcgaac tgtatttttc aaatgttaga attcttgctt cgcaatatcg
      241 ttttttcttt ttagtaattt gtagagttac tatatcattg ggaatagctc cttctacaaa
      301 aataataagg tcatcaactt ttgctatagc ttttccttca gctgcaatgt caattatttg
      361 tacatgttca agaatcggaa aatttttttt tcgccccaca gttctattca ttaatttatt
      421 tgcaaatatt catatatttg taataattat tgatgaattc atggaaatat ttacacattt
      481 attattagga gcaatagtag ccggcttatt tggagcaagg tatattggaa attgggcttt
      541 tttatggggt gcattattgt cattaatccc aagtattgat atgctcattt taatattttc
      601 cgattctttg gaagggttaa attttttacg tggaatcact cattctattc tttttatact
      661 tgttatgtct ccacttgcag caattttatt gagtaaaatt acttttcatt caactattac
      721 aattgctcaa tggacacgtc ttatattact tgtatttgca tcgcatgtag catatgatat
      781 ttttacaatt cagggagttg ggcttttaga accgtttact gataaacggt atgctctatg
      841 ttcaattgct gatttcgatt tattttttgt gataccttta ttggcggctt tggcattggc
      901 tcttaaagtt tcacaaaaca gacataaaac aataattgta tggtttggaa tttttatttc
      961 agtattgtat atatctttta catttttaaa caagttgtat attcaatctg aatttgaaaa
     1021 aaaattagtt gccgaaaaaa tcagatttga taggactgaa atttttcctg tgcttggcag
     1081 taattttttg tggaattgta tagctcagga tagagatggc ttttggatgt gttatgaaac
     1141 aaattttagt aaacacaatt ttgaaatgaa tctgtatatg cgtaatgatt attatatttt
     1201 tgattttgaa aatgattcgc gtattcaaaa aatcaaatca agcactaaag attattatat
     1261 tgttcaaaaa atgggtaacg gagatgtgtt tatatatgat ttacgtatgg gacgaatggg
     1321 attaaattca caagctcctt ttatgtattc gtatagaata aaaaatatgc atgggaccat
     1381 tcaatcttta gaaaaaatag aaccttcatt ttattcaata ttttttaaaa aaagtaaata
     1441 agattcattt tataaatgaa tcataatagg cagaaatttt atatggcttt gtatataaaa
     1501 gttctgcatt cccttgagta caagcaaa
//
LOCUS       DAOTOW010000621         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_65346_length_1520_cov_2.391126, whole genome shotgun sequence.
ACCESSION   DAOTOW010000621 DAOTOW010000000
VERSION     DAOTOW010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65346_length_1520_cov_2.391126"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1349)
                     /locus_tag="P5243_09840"
     CDS             complement(156..1349)
                     /locus_tag="P5243_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRS19794.1"
                     /translation="MTHTIEQYIAECGDHVLHAFSALPRPSKKLEDWKFFTHTEWFEK
                     QWNITTAYQLTSDFIKEQLQGIDYDSLLVFHNGSYESQHSIVSDFIGVSSQEYKPIFE
                     SKSLTQLDIVHTANAKRGINIRIANTDKSSHIVLLSVVSGAHTLCTASHTIHVETNAQ
                     CTITEIIVSEAHSAESLMLTGTQLICNAGSVCEYTNIQKLHNSSSVLQSIAVLQLQQT
                     TTSVHVYPLSGAYNRTNMSVYKQGTQANTNVYGILFPCNDEHVELYTNIYHNVPECET
                     NEQVKGLAKDDGQGVFSGLIYVAQDAQKTRAAQSNKNMLLSSTAKIHSKPQLEIYADD
                     VSCNHGSTTGQINKEALWYMRARGIDTNEATRLLLDGFINEVLEKITRQDILQIILHD
                     LELKM"
     gene            complement(1377..>1520)
                     /gene="sufC"
                     /locus_tag="P5243_09845"
     CDS             complement(1377..>1520)
                     /gene="sufC"
                     /locus_tag="P5243_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618681.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRS19795.1"
                     /translation="ITHYQRLLDYIVPDVVHIIWDRKIIKTRDKSRELELEERGYDWI
                     TKS"
BASE COUNT          455 a          275 c          247 g          543 t
ORIGIN      
        1 ctcaattgac tttggtacat ggtattataa aaacgatcca actcatgtaa gcatattttc
       61 aaaaaaaaca tgtgaatgga ttgctcatac ctatggcttt acacactttg aaatacaaaa
      121 cagagttata ctattttcaa aataatatgt taatattaca tttttaactc caaatcatga
      181 agtattattt gtaatatatc ttggcgcgtt attttttcaa gaacttcatt tataaaccca
      241 tctagtaata agcgtgtcgc ttcatttgta tcaattcctc gagctcgcat ataccataat
      301 gcttctttat taatttggcc ggttgtacta ccatgattac aacttacatc gtcagcatat
      361 atttctaatt gcggtttact atgtattttt gcagtagatg acaaaagcat atttttatta
      421 ctttgtgcag cgcgtgtttt ttgtgcatct tgtgccacat atatcaatcc cgaaaatacc
      481 ccttgaccgt cgtctttggc tagccctttc acttgttcat ttgtttcgca ctcgggaaca
      541 ttgtgataaa tattggtata gagttctaca tgctcatcgt tgcagggaaa caatattcca
      601 tatacattgg tattggcttg tgtgccttgt ttgtaaaccg acatatttgt tctattatat
      661 gctccgctta aaggatagac atgcactgaa gttgtagttt gttgcaattg taatacggca
      721 atactttgta atacagatga actattatgc aatttctgaa tatttgtata ttcacataca
      781 gaacctgcat tgcaaatcaa ttgagttccg gtaagcataa gcgattctgc agaatgagct
      841 tcggaaacga taatttctgt aatagtacat tgcgcattag tttctacatg aatcgtatgt
      901 gatgcggtac acaaggtatg cgctccacta accaccgata gtaatacaat atgacttgat
      961 ttatcagtat ttgctattcg aatattaatc ccacgtttgg catttgcagt atgcacaata
     1021 tcgagctgtg taagactttt gctttcgaat attggtttat attcctgtga cgaaacacct
     1081 ataaaatcgc taacaataga atgctgcgat tcatatgaac cattgtgaaa tactaacaac
     1141 gaatcgtaat ctattccttg taattgttcc ttaataaaat cagaagtcaa ttgataggca
     1201 gttgtaatat tccattgttt ttcaaaccac tctgtatggg taaaaaattt ccaatcctca
     1261 agttttttac ttgggcgagg caatgcagaa aatgcatgca acacatgatc gccacattct
     1321 gctatatatt gttctatggt atgtgtcata ggtatagtgc tataatatac aacatgttac
     1381 gatttagtaa tccaatcgta tcctctttct tctaattcca attcacgact tttgtctcgg
     1441 gtttttatga tttttcgatc ccatattata tgcactacat cgggtacaat ataatcaagt
     1501 aatcgttgat agtgcgtaat
//
LOCUS       DAOTOW010000622         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_65721_length_1514_cov_4.401645, whole genome shotgun sequence.
ACCESSION   DAOTOW010000622 DAOTOW010000000
VERSION     DAOTOW010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65721_length_1514_cov_4.401645"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="P5243_09850"
     CDS             <1..448
                     /locus_tag="P5243_09850"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19796.1"
                     /translation="DGQGFLAVNYDGFIPILWESHTEMLHRIQALEAEIENLTKNNGK
                     NKLKSESITEETHATIQSSIVLHQNFPNPWNSSTTIAATIPAHVTSAMVCIYDLTGKQ
                     LRCYHIAQRGEAQIVLHAEELQPGMYIYSLLADGKLIDSKQMVLTE"
     gene            460..1443
                     /locus_tag="P5243_09855"
     CDS             460..1443
                     /locus_tag="P5243_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19797.1"
                     /translation="MKYIVFVMLFVSGIQQTNAQIFNDWKSLPLDHYIIKFVPYNPIS
                     LDGIVTQNTKYWWENEPDRQCLEFSIEFGSNTNYDVEADGYNKDYQNYWNKATRIMTN
                     NNFGEENYISIGWRYNITSQMLELGFYGHRDHSDSQPRRIYEQLCTKPSFSTCNVQMA
                     LTKELTYLNIDGYALLTIKNVQSWDIREEIEIQAKWGWYGDIVPYQYLSHDFKYIHTR
                     DFKRDRESFWNNAEFTYASNSLKIAHSRIPKDFPYRTFISNKLYLSHLCESTNNLYNY
                     TDEENVSDSQKGSFLIIEGGGNPIQFIANETIIGGNTIIEKGTQVYIGPIF"
BASE COUNT          571 a          217 c          259 g          467 t
ORIGIN      
        1 agacgggcaa ggatttttgg cggtaaatta tgacgggttt attcctattc tatgggaatc
       61 gcataccgaa atgctccaca gaattcaagc gttagaggca gaaatagaaa atcttaccaa
      121 aaataacggt aaaaacaaac tgaaatcaga atcaattacc gaagaaacgc acgcaactat
      181 tcaaagttcg atagtattac atcaaaattt ccccaaccct tggaattcaa gcactactat
      241 agcggcaact attcctgcac atgttacaag tgctatggtg tgcatttacg accttaccgg
      301 caaacaatta cgttgttacc acatagcaca acgtggcgaa gcacagatag tattacatgc
      361 cgaagaatta caacccggta tgtatatata ttcattgctt gccgatggta aattgattga
      421 tagtaaacaa atggtgttaa cggagtaata tatatagata tgaaatacat agtttttgtt
      481 atgctatttg taagtggaat acaacaaacc aatgcacaaa tatttaatga ttggaaaagt
      541 ttaccgctcg atcattatat tattaaattt gttccatata atccaattag tttagatggt
      601 atagttacgc aaaataccaa atattggtgg gaaaatgaac cggataggca atgtttagaa
      661 ttttcgatag aatttggctc gaatacaaat tatgatgttg aagcagatgg atacaataag
      721 gattatcaaa attattggaa taaagcaacc cgaataatga caaataataa ctttggagaa
      781 gaaaattata taagtattgg atggcgatat aatataactt ctcaaatgct ggaattaggt
      841 ttttatggac atagagacca tagtgatagt cagccacgta gaatttacga acagttatgt
      901 actaaaccaa gtttttcaac ttgtaatgtt caaatggcat taactaaaga attaacgtat
      961 ttgaacattg atggatatgc attgttaact attaaaaatg ttcaatcttg ggatataaga
     1021 gaagagattg aaatacaagc aaaatggggt tggtatggtg atatcgttcc atatcaatac
     1081 ctttcacatg actttaaata tatccataca agagatttta aacgagatag ggagagtttt
     1141 tggaataatg cagaatttac ctatgcaagc aatagtttaa aaatagcaca ttcacgtatt
     1201 cccaaagact ttccatatag gacattcatt tcaaataaac tatatttaag tcatctatgt
     1261 gaaagtacaa ataatttata taattacact gatgaagaaa atgtgtccga ttctcaaaaa
     1321 ggttcatttt taattattga gggtggtggt aatcctatac aatttattgc caatgaaaca
     1381 ataattggcg gtaatacaat tattgaaaaa ggaacacaag tatatatcgg acctatattc
     1441 taaataatac attttaggtt tttaacctat aatatttttt ttaaacaaaa gtatttcatt
     1501 tatgaattca ataa
//
LOCUS       DAOTOW010000623         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_65801_length_1513_cov_2.679698, whole genome shotgun sequence.
ACCESSION   DAOTOW010000623 DAOTOW010000000
VERSION     DAOTOW010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65801_length_1513_cov_2.679698"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="P5243_09860"
     CDS             <1..456
                     /locus_tag="P5243_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRS19798.1"
                     /translation="CTPVADDLRTGMTINNILPSLERISDVSEKIAKFARKYPQPFEQ
                     ELIEKTGLHNLMASLLFMYNSVVESFADNSTLKLNDVFVKDKEINAIYKSAKKTIIEL
                     LQESSLDSEKKVNLLLIISKLERAGDHIKTIAEEIYFCVEGVFMRHKHE"
     gene            518..1102
                     /locus_tag="P5243_09865"
     CDS             518..1102
                     /locus_tag="P5243_09865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRS19799.1"
                     /translation="MSIVTTLSLIRHGQTDWNAVGKLQGREDIPLNDFGREQAKKLAH
                     YFSLQQWDCIVTSPLQRAFETARIISNSLQNIQIHTNALLVERCWGQASGLTYEQRKE
                     TFPDGIIPQQEEFEPLQQRAMQAMNDIIRQHEGKHVIVVSHGALINAVLHAVSHGEFG
                     SFKTRLANACISILQYNNSSWNVLFYNKTVDELL"
BASE COUNT          577 a          200 c          260 g          476 t
ORIGIN      
        1 tgtaccccgg tagctgatga tttacgtact ggcatgacta ttaataatat tttaccgtca
       61 ttagaaagaa ttagtgatgt atcagaaaaa atcgcaaaat ttgctagaaa atatccacaa
      121 ccttttgaac aggaattaat agaaaaaaca ggattacata acttaatggc aagtttatta
      181 tttatgtata attctgtagt agaatcgttt gctgataata gtacacttaa attaaacgat
      241 gtatttgtaa aagataaaga aataaatgct atatataaaa gtgctaaaaa aacaatcatt
      301 gaattattgc aagaatcatc gttggatagt gagaaaaaag ttaatttatt attaataata
      361 tctaaacttg aacgtgccgg tgaccatatt aaaacaattg ccgaagaaat atatttttgt
      421 gtagaaggtg tatttatgcg tcacaaacat gaataaatcg tattattttg taattcaaat
      481 ataatagtag atttgcataa atctaaattg tatttgcatg tcaatagtta ctactcttag
      541 tttaatacgt cacggccaaa ctgattggaa tgcagttggt aaattacaag gaagagaaga
      601 tattccgctc aacgattttg gtcgtgaaca agcaaaaaaa ttagctcatt atttttcttt
      661 acaacaatgg gattgtatag taacaagccc attgcagcga gcctttgaaa cagcacgcat
      721 tatttcaaat tctttacaaa atattcaaat acatacgaat gctttgttgg tggaacgttg
      781 ttgggggcaa gcttcaggat tgacatatga acaacgaaaa gaaacttttc ccgacggaat
      841 tattcctcag caagaagaat ttgaaccgct tcaacagcgt gctatgcaag ctatgaatga
      901 tattataagg caacatgaag gtaaacatgt tatagtagta tcacatggag cattaatcaa
      961 tgctgttttg catgccgtat ctcatggaga atttggaagt tttaaaacac gattggcaaa
     1021 tgcttgtatt tctattttac agtataataa ttcctcatgg aatgttctat tttataataa
     1081 aacggttgat gaattactat agagaagtag aaaatagtat ttgatattgt gtattagtat
     1141 gtatgtcact aacatacttc tatgccttat aaatattgat attctaattt tttttctaat
     1201 gtaaccacct tgtttttaat aaattgttaa aatccaataa atttttataa aaaaaaagta
     1261 acaaacaatt tggaaaatag aaataagaat atatatcttt gcagccccaa aaaacaggta
     1321 ggagtacttg gtaaaaagca aaggggaagt tcattgaaac atatatataa gccaagataa
     1381 ggtattcata gagaataaaa gatgagcgaa ctaggattat tcgtgtaaag aatataagat
     1441 aaagaaaata attacaacga agagtttgat cctggctcag gatgaacgct agcggcaggc
     1501 ttaacacatg caa
//
LOCUS       DAOTOW010000624         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_66129_length_1508_cov_3.383345, whole genome shotgun sequence.
ACCESSION   DAOTOW010000624 DAOTOW010000000
VERSION     DAOTOW010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66129_length_1508_cov_3.383345"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..>1508
                     /locus_tag="P5243_09870"
     CDS             304..>1508
                     /locus_tag="P5243_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19800.1"
                     /translation="MKIRILAILFCLQLCSTIAFTQTKEYRSAARVESFVKEIFPEAF
                     DEMLLRDACLYYINKELTKNNTRILVPNETLQTISQQFAVYMSDVDDTRATYAPKKYQ
                     LENRLIEAGAGIHQADEVTIKTLVAKGKTLLTYDEVAQEVAFQFFKRKSAEVLMHPKY
                     VFAGIGCKLDKTNKKVFVAVSVGNYNLLSMDKKAIKESGLPINQSQHGLEGYDARTCK
                     PCERYKNISQLQEGITVENGEIYYSTSDYKSLRKILKYPKDGLAVDIVLENQYPCKGA
                     NIVNKQLQTRGTLTKPVYFSNFEKLNLETGRNASTKLKLKMGTLPEGIKDYELNLVII
                     KDKRVCKNIFPAYTKQFETAEKLDLQPFPDTVTKYNTFIYKPKYEIDTITFIIPFEVK
                     ITKAKDGDKG"
BASE COUNT          572 a          189 c          239 g          508 t
ORIGIN      
        1 gagaaaggaa gactaacatc ttccttctct taatttttta tcggacaaca gtgacttttt
       61 ttgttttacc tctaaggaat aaaaaaatag tttatttata tagtaaattt ttttatacat
      121 gttcacaatt ggtttataaa aaaatatagt tgtgaaatta gatataaata atagaaaatc
      181 aagttattgc atattttgtt ctaatgattt tgcttaatta agcgtaattt tatatttgtt
      241 aatatcgtat ttttttgtac gtttgttact ttcacaaagt ataatatttt acaagcgata
      301 tatatgaaaa ttagaatttt agctatctta ttttgtttac agttgtgttc aacaattgca
      361 tttacacaaa ccaaagaata tagaagtgct gcccgtgttg agtcttttgt taaagaaatt
      421 tttccggagg cttttgatga aatgctattg cgtgatgcgt gtttatatta tataaataaa
      481 gagttaacaa aaaacaatac ccgaatttta gtacctaatg aaacattgca aactatttct
      541 caacaatttg cggtatatat gagtgatgtt gatgatacaa gagctacgta tgctccaaaa
      601 aaatatcaat tagaaaatag actcattgaa gcaggtgccg gaatacatca agctgatgaa
      661 gttactataa aaaccttagt agctaaaggt aaaaccttac tcacatatga tgaagttgct
      721 caggaagttg catttcagtt ttttaaacgt aaatcggcag aagttcttat gcatcctaaa
      781 tatgtttttg ccggtatagg gtgtaaatta gataaaacaa ataaaaaagt ttttgttgct
      841 gtaagtgttg gtaattataa cttgttaagt atggataaaa aagccattaa agaatcgggc
      901 ttaccaatta atcaatcaca acatggactt gagggctatg atgctcgtac atgtaaacca
      961 tgtgaacgat ataaaaatat aagtcagtta caagaaggaa ttactgttga aaatggtgaa
     1021 atttattatt ctacatcaga ttataaatcg ttgcgaaaaa ttttaaaata tcctaaagat
     1081 ggattagccg tagatatagt tcttgaaaat caatatccat gtaaaggtgc taatattgta
     1141 aataaacaat tgcaaacacg aggaacactt acaaaaccgg tatatttcag taattttgaa
     1201 aaactaaatc ttgaaacagg cagaaatgct tcaactaaat tgaaacttaa aatgggtaca
     1261 ttgcccgaag gaattaaaga ttatgaattg aatttggtta taattaaaga taaacgtgta
     1321 tgtaaaaata tttttccggc atatactaaa caatttgaaa cagccgaaaa gcttgattta
     1381 caaccattcc ctgatacggt tactaaatat aatacgttta tttataaacc aaaatacgaa
     1441 atagatacta ttacttttat tattccattt gaagtgaaaa taactaaagc taaagatgga
     1501 gataaagg
//
LOCUS       DAOTOW010000625         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_66183_length_1507_cov_3.442837, whole genome shotgun sequence.
ACCESSION   DAOTOW010000625 DAOTOW010000000
VERSION     DAOTOW010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66183_length_1507_cov_3.442837"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="P5243_09875"
     CDS             complement(<1..755)
                     /locus_tag="P5243_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19801.1"
                     /translation="MSERIIEALLKLFAVFARPDTSTSDKQDVVELFLAAQLNKELAI
                     NYLPTFKQEYEEVVADISRITEKAGSKTGDALKYKIEAKLAARIHRICNSINQELTQK
                     QKLFVIISILEFISSDKEYNGEITTSEKEFLAILIDDLNIDEQEYAQLSSFILSPFDE
                     IVETTNVLLINNNEHHTSTESKHIYSPHLSGCIKVFYIASTNLLLFRSQGVGEITLNS
                     QPVHENKVNFISIGSAIRSHQIKPIYYSEILNK"
     gene            complement(760..1179)
                     /locus_tag="P5243_09880"
     CDS             complement(760..1179)
                     /locus_tag="P5243_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19802.1"
                     /translation="MKQLRIIIVLVCAFGFHNSMWAQQKKSAIDLCDNYMNDGFISDG
                     QYYSHSIQKDEIKQTKITLMGGNTYRIAACAEQSKQLQFSIIDVQGNILFTNTQFNYA
                     PYWDFEIKHTIECTITLQNNDKTLPKDKTILIIGYKR"
     gene            complement(1180..>1507)
                     /locus_tag="P5243_09885"
     CDS             complement(1180..>1507)
                     /locus_tag="P5243_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19803.1"
                     /translation="LLTQINKEKPNKKIIEKIAEHKYYADNVLALLRPYYDNSEEHKK
                     IIDDIVEICYQFDGVTSNYTYVEPITYTQLKRTVIQSQTKLDIFNEHLQNITIFTEKL
                     RNTIIK"
BASE COUNT          450 a          199 c          239 g          619 t
ORIGIN      
        1 aatttattga gaatttcact atagtatata ggttttattt ggtgactgcg aatagcagaa
       61 ccaatcgaaa taaaattgac tttattttcg tgtaccggtt gactgttgag tgtaatttcg
      121 ccaactcctt gtgaacgaaa cagtaataag tttgtagaag ctatataaaa tactttgata
      181 caacctgata aatgaggcga ataaatatgt ttagattctg ttgatgtatg gtgttcatta
      241 ttatttatta acaatacatt ggtagtttct acaatttcat caaaaggact taaaataaat
      301 gaacttaatt gagcatattc ttgttcatct atgtttaaat cgtctattaa aattgcaaga
      361 aattcttttt cgcttgtagt aatttcgccg ttgtattctt tgtcagaact tataaactca
      421 agaatactta ttatgacaaa taatttttgt ttttgggtta attcttggtt tatactgttg
      481 catatacggt gaatacgagc agctaatttc gcttctattt tatatttgag tgcatcaccc
      541 gtttttgaac ctgctttttc ggtaattcgg cttatgtctg caactacttc ttcgtattct
      601 tgtttaaacg taggcaaata atttattgct aattctttat ttaattgtgc agcaagaaat
      661 aattccacta catcttgttt gtcactggtt gaagtatcgg gacgagcaaa tacggcaaat
      721 agttttaata atgcttctat aattctttca ctcatagttt tatcgtttgt atcctattat
      781 tagaattgtt ttgtctttgg gtaatgtttt atcgttgttt tgtaaagtta tggtacattc
      841 aattgtgtgt ttaatttcaa aatcccaata gggagcatag ttaaattgag tattggtaaa
      901 taatatattt ccttgaacat caattattga aaactgtaat tgtttacttt gttcggcaca
      961 tgctgctatt ctataggtat tgcctcccat gagggtaatt ttagtttgtt ttatttcatc
     1021 tttttgaata gaatgtgaat aatattgccc gtcagaaata aatccatcgt tcatgtaatt
     1081 atcacataaa tcaattgcag attttttttg ttgtgcccac atagaattgt gaaaaccaaa
     1141 agcacatacc aaaactataa tgatacgtaa ttgtttcatt tatttaataa tagtatttct
     1201 tagtttttcg gtaaaaatag ttatattttg taaatgttca ttaaaaatat ctaatttggt
     1261 ttgcgattga attacagtcc tttttaattg agtatatgta ataggttcta catatgtata
     1321 gtttgatgta acgccatcaa attgataaca aatttcaact atatcgtcaa ttattttttt
     1381 gtgttcttct gaattgtcgt aatacggacg caacagagca agtacattat cggcatagta
     1441 tttatgttcg gctatttttt caattatttt tttgtttggt ttttctttgt ttatttgagt
     1501 aagtagg
//
LOCUS       DAOTOW010000626         1499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_66656_length_1499_cov_3.238920, whole genome shotgun sequence.
ACCESSION   DAOTOW010000626 DAOTOW010000000
VERSION     DAOTOW010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66656_length_1499_cov_3.238920"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /gene="asnA"
                     /locus_tag="P5243_09890"
     CDS             <1..760
                     /gene="asnA"
                     /locus_tag="P5243_09890"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRS19804.1"
                     /translation="EYEFPPGYGILTDMNAIRPDEDLDNIHSLYVDQWDWERTMKENE
                     RDLDFLKYIVKKIYEVLKRTEFIISEEYPQIIPILPEEITFFHAEELLQMYPDKTPKE
                     RETIVTKQYKAVFVIGIGHELANGEKHDGRAPDYDDWSTPTGNGNVGLNGDILLWNPI
                     LESAFEISSMGIRVNPEAMNYQLQKAYAEDRKNLLFHSKLLNGELPQSIGGGIGQSRL
                     CMFFLRKAHIGEVQSALWPDDMRLECKKNNIILV"
     gene            complement(1123..>1499)
                     /locus_tag="P5243_09895"
     CDS             complement(1123..>1499)
                     /locus_tag="P5243_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRS19805.1"
                     /translation="LSNKREAFTEDLAIRLQNVQIECADTNYVIRSRDFEDAFFYVDP
                     PYIGTCMGHYDGYSEDDYEKLLKTLSQIKGKFLLSSFPTDILKRFTSQHGWHTKEVEM
                     RCSASKVGKRKVEVLTANYTLK"
BASE COUNT          531 a          286 c          258 g          424 t
ORIGIN      
        1 agaatatgaa tttcctccgg gctatggtat tttaaccgat atgaacgcca ttcgtcccga
       61 tgaagatttg gataatattc attctttata tgttgaccaa tgggattggg aacgaaccat
      121 gaaagaaaat gaacgtgatt tagattttct taaatacatt gtaaaaaaaa tatacgaagt
      181 actcaaacgt acagaattta ttatttctga agaatacccg caaataatac ccattcttcc
      241 tgaagaaata actttttttc atgcagaaga gttactgcaa atgtaccccg acaaaacacc
      301 aaaagaacgt gaaacaatag taaccaaaca atacaaagct gtttttgtaa taggtatagg
      361 tcacgaattg gccaatggtg aaaaacacga cggcagagcg ccagattatg atgactggag
      421 tacacctacc ggcaatggta atgtgggact aaatggtgat attttgttgt ggaatcctat
      481 attggaaagc gcattcgaaa tttcatctat gggaatacga gtaaaccccg aagctatgaa
      541 ttatcaactg caaaaagcat atgccgaaga cagaaaaaac cttttatttc attcaaaatt
      601 actcaacgga gaactaccac aatctattgg cggcggtata ggacaaagcc gtttgtgcat
      661 gttctttttg cgcaaagcac atataggcga agtgcaaagt gccctttggc ccgatgacat
      721 gcgactggaa tgcaagaaaa acaatataat attagtataa tcacaaaagc ctcgaataaa
      781 attcggggct tttttttaaa taatactgtt gcacctacga agctaaatga atacataatg
      841 ttaaacggtt caagccttgg agaggcgaaa ctatggtaga aaaaaaatat atgcattgta
      901 aaataagcct cagcgaggcg gcattaattt gtgtataaat actgtcctat aatgccccat
      961 atatttatta catgcgacct accatattac cgctcctacg gagcttgttc aaataaatat
     1021 ataatcgcaa taacaaaatg tacacttttc aaaaacgaaa tgtacaaaaa aattccacac
     1081 gcacacacaa tcgcacgcat ggaatttaaa tatcaattaa atctatttta aagtataatt
     1141 agcagttaat acctcaacct ttcttttacc aactttacta gcactacaac gcatctcaac
     1201 ctctttggtg tgccaaccat gttgtgaagt aaatcgtttt aatatatcag taggaaaaga
     1261 agacaataag aacttacctt ttatctggga taaagtcttt agaagctttt cgtaatcatc
     1321 ttcgctatat ccatcgtaat gccccatgca tgtgccaatg tatggcggat ccacataaaa
     1381 gaatgcatcc tcaaaatctc tcgagcgtat tacatagtta gtatcggcac actctatttg
     1441 cacgttttgc aaacgtattg ccaaatcttc agtaaacgcc tcacgtttgt tagataatc
//
LOCUS       DAOTOW010000627         1497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_66803_length_1497_cov_2.806519, whole genome shotgun sequence.
ACCESSION   DAOTOW010000627 DAOTOW010000000
VERSION     DAOTOW010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66803_length_1497_cov_2.806519"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="P5243_09900"
     CDS             complement(<1..876)
                     /locus_tag="P5243_09900"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS19806.1"
                     /translation="MIKQLQRTIPPKYTTLQLKRFVNPQKYVCPNNIPIYTIDAGDEE
                     IVKIDIVFEAGDWHQPQSVVATSVVSLLQEGSLHFSAEEIAEKLDYLGSYLYTSASKH
                     TATISVYCLHKHFKETIKIIEDIIKNPTFPEDKIQTFIHKKHQQYSIEAQKVEVISQK
                     KFASILFGGFHPYGISAEPEDFLKINKDICHSFHKEFYNSDNCKIIVAGKIKNNHIDI
                     LKKHFGLLTWNVREIVSQIPQYVITPSFDKKHYFKKEDAVQSAIRVGKMLINKLNPDY
                     PALQVLNTILGGYFGS"
     gene            complement(890..>1497)
                     /locus_tag="P5243_09905"
     CDS             complement(890..>1497)
                     /locus_tag="P5243_09905"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS19807.1"
                     /translation="YREISPILIPEEPQQTEKRTLSVERNVPVNSIYKVFHMCNRIHD
                     DFYATDIISDILSNGKSARLYKHLVQEQHIFSEINAYITGEVDEGLFVISGNIMPGIS
                     MEVAEQKIEFELQQIIEGIITEYELQKIKNKIESTIIFSETNVLNKAMNLAKFMHLGD
                     ISLIQKEIKKYSNVSIEQIQNVAKQLFNTNNDSTLYYYARK"
BASE COUNT          484 a          206 c          225 g          582 t
ORIGIN      
        1 ggagccaaaa tatccgccta atattgtatt taaaacttgt aatgcaggat aatcggggtt
       61 aagcttattt ataagcattt tacctacacg aatagcgctt tgaacagcat cttctttttt
      121 gaaataatgt tttttatcaa aacttggagt aataacatat tggggaattt ggcttacaat
      181 ttcacgtacg ttccaagtca ataagccaaa atgtttttta agtatgtcta tatgattatt
      241 tttaattttg cctgctacta ttattttgca attatctgaa ttatagaatt ctttatgaaa
      301 agaatgacaa atgtctttat ttatttttaa aaaatcttcg ggttctgctg aaataccgta
      361 aggatgaaaa cctccaaata aaattgatgc aaattttttc tgtgaaatca cttcaacctt
      421 ttgagcctca atagaatatt gttggtgttt tttatgaata aatgtttgaa ttttatcttc
      481 ggggaaagta ggatttttta taatatcttc aattatttta attgtttctt taaaatgttt
      541 atgtaaacag taaaccgata tagttgctgt atgttttgat gctgatgtat acaagtacga
      601 acctaaataa tctaattttt cagctatttc ttctgctgaa aaatgtaaag aaccttcttg
      661 taataaagaa acaaccgaag ttgcaactac agactgtggt tgatgccaat cacctgcttc
      721 aaatacaata tcaattttaa caatttcttc atcaccggca tcaatggtat atattggaat
      781 attattgggg catacatact tttgcggatt aacaaatcgc tttaattgca atgttgtgta
      841 tttaggaggg atagttcgct gtaattgttt tatcataaat gttcaatagt tatttacgtg
      901 cataataata aagtgtggaa tcattatttg tattaaataa ttgtttagca acattttgta
      961 tttgttcaat tgaaacatta ctgtatttct ttatttcttt ttgtataaga gaaatatcgc
     1021 ccaaatgcat aaatttagca aggttcatag ctttattcaa tacattagtt tcactaaaaa
     1081 taatagttga ttctattttg tttttgattt tttgtagttc atattcagtg attatacctt
     1141 caataatttg ttgtaattca aattctattt tttgttcggc aacttccatt gaaataccag
     1201 gcataatatt ccctgatatg acaaataatc cttcatcaac ttcaccggta atataagcat
     1261 ttatttcgct aaagatatgt tgttcttgaa ccaagtgttt atataaacgg gccgatttac
     1321 cattagataa aatatcagaa ataatatcag tagcataaaa atcgtcatga atacgattac
     1381 acatgtgaaa caccttgtaa atagaattta caggaacatt tctttcaaca gataatgtgc
     1441 gtttttctgt ttgttgtggt tcttctggta tgagtatggg tgaaatttct ctatatg
//
LOCUS       DAOTOW010000628         1495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_66946_length_1495_cov_2.581250, whole genome shotgun sequence.
ACCESSION   DAOTOW010000628 DAOTOW010000000
VERSION     DAOTOW010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66946_length_1495_cov_2.581250"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..358)
                     /locus_tag="P5243_09910"
     CDS             complement(98..358)
                     /locus_tag="P5243_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19808.1"
                     /translation="MNIFSSEILQNTVAYPLVEKITADENNYVIYMAIPTGEDTTKCS
                     IKRVTYTKNGTAETWITQYPFGKKHAEFDWDLRAEYTYKQAI"
     gene            complement(363..902)
                     /locus_tag="P5243_09915"
     CDS             complement(363..902)
                     /locus_tag="P5243_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRS19809.1"
                     /translation="MVSITEIKQLSVDTAKGGMLPSVFAAQAILESGVFKGGSTLARN
                     YNNYFGIKKGSSWTGKTVTLPTKENYNGIDVYIKDEFRVYDTPRQSFEDRVNLFLNSS
                     RYKDVYTYDTPEEQAKALQRAGYATDPNYASKLIQIIDTHNLRELDKKKKNMKTIQII
                     VLVLALSIVVATTYSLVTG"
     gene            complement(902..1066)
                     /locus_tag="P5243_09920"
     CDS             complement(902..1066)
                     /locus_tag="P5243_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19810.1"
                     /translation="MNDFLQSKVVQDFKDGKLPVVETVVEFDIPSLAYLGITLLIVGF
                     ILLVGKKMLA"
     gene            complement(1071..1355)
                     /locus_tag="P5243_09925"
     CDS             complement(1071..1355)
                     /locus_tag="P5243_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRS19811.1"
                     /translation="MKTNWTLWIVISLLIITAVIVYVMVQKQKNPGVFPLKQGSRGEE
                     VRKLQEWLNKQNAGITIAVDGIFGPKTESLLFAVTGAKEISSKEYKSKIA"
BASE COUNT          414 a          283 c          232 g          566 t
ORIGIN      
        1 gcatgaaagt tggtttgcat ttgcaacaat tcgctgtttg atttaccttc taaatcaaat
       61 tttccggtaa gtgggttaaa tcctaaatta gacatagtta tatagcttgt ttgtatgtat
      121 attctgcacg taaatcccaa tcgaattcag catgtttttt gccgaatgga tattgagtaa
      181 tccatgtttc tgcagtacca tttttggtat atgtaacgcg ttttatacta catttggtag
      241 tatcttcgcc ggtaggaata gccatgtata taacatagtt attttcgtct gcagtaattt
      301 tttccacaag tggatatgct accgtgtttt gtaatatttc gctcgaaaat atattcattt
      361 cattatcctg ttacaagtga ataggtagtt gctaccacta tagataaagc aagtaccagt
      421 actataattt gaattgtttt catatttttt ttctttttgt caagttcgcg taaattatga
      481 gtatctataa tttgtataag tttgcttgca taatttggat cggttgcata tcctgcccgt
      541 tgcaatgctt ttgcttgttc ctcgggtgtg tcgtaggtat atacgtcctt gtatcggctg
      601 gaattcagaa ataaatttac tctatcctca aatgattgcc ggggcgtgtc gtatactcta
      661 aattcgtctt taatgtatac atctataccg ttatagtttt cttttgttgg aagagtaacc
      721 gttttaccag tccaggaact acctttttta ataccaaaat aattattgta attgcgagcc
      781 aatgtactgc cacctttgaa tactccactt tcgagtattg cctgtgctgc aaatacacta
      841 ggaagcatac cgccttttgc agtatctaca cttaattgtt ttatttcggt aatcgatacc
      901 attatgctaa cattttttta cctaccaaca atataaaccc tacaattaat agcgtaatac
      961 ctaaatacgc caacgatgga atatcaaatt ccactaccgt ttctactacc ggtagtttac
     1021 cgtctttaaa gtcttgtaca actttacttt gcaaaaaatc attcatgcca ttatgctatt
     1081 tttgatttat actcttttga actaatttct tttgctcctg ttactgcaaa caataaactt
     1141 tctgttttag gaccaaatat gccatctaca gctattgtaa tgcctgcatt ttgtttgttg
     1201 agccattctt gtaattttcg tacctcttcg cccctactac cttgtttgag cggaaaaacg
     1261 cccggattct tttgtttttg aaccattaca tatacaatta cagcagttat aatcaagagt
     1321 gaaataacta tccaaagtgt ccaatttgtt ttcatatact accctattaa atgcttacac
     1381 ctatttgcga taaaatttta tttgcttctt gtttttctgc attatcaagt tcgtatgcaa
     1441 gatgatcgta caaattgccc tcaatggtaa accatagagt accggtataa atacc
//
LOCUS       DAOTOW010000629         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_67042_length_1493_cov_3.589708, whole genome shotgun sequence.
ACCESSION   DAOTOW010000629 DAOTOW010000000
VERSION     DAOTOW010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67042_length_1493_cov_3.589708"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="P5243_09930"
     CDS             <1..238
                     /locus_tag="P5243_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRS19812.1"
                     /translation="RNGAKEYTRMQFAQFGMMNPSDEDLEHWSKEIIKNRDQANRIVE
                     QEQDKKLIAFFKETVKLNEVEKTIEEFNAMFEKK"
     gene            352..1014
                     /gene="clpP"
                     /locus_tag="P5243_09935"
     CDS             352..1014
                     /gene="clpP"
                     /locus_tag="P5243_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRS19813.1"
                     /translation="MNDFRKYATKHLGISSVSLDKYTSVLNNYISPTIIEERQLNIAS
                     MDIFSRLMMDRIIFLGLPIDDYVANIVQGQLLYLESADSSRDIQIYINSPGGSVYAGL
                     GIYDTMQYIQADVSTICTGMAASMAAVLLAGGTKGKRLALNHSRVMIHQPLGGAQGQA
                     TDIEITTREILKLKRELYEIIAHHTGHEYAKIEKDSDRDYWMTSHEAKEYGMIDDVMM
                     RK"
     gene            1042..>1493
                     /locus_tag="P5243_09940"
     CDS             1042..>1493
                     /locus_tag="P5243_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRS19814.1"
                     /translation="MKQILVHTCCAPCASASVERLIANGWQPVLYFYMPNIFPFEEFE
                     KRLSYVQKLADYYNIQLIVGDYNHSNWLGAIKGTESAPEGGARCEICYKTLLQATSIK
                     AQELEILHFCTTLTISPHKNSKKIEEIGSQYQGYTHYDFKKQDGYKRS"
BASE COUNT          539 a          235 c          280 g          439 t
ORIGIN      
        1 gcgtaatgga gcaaaagaat atacacgtat gcaatttgca caatttggta tgatgaatcc
       61 aagtgacgaa gatttagagc actggagcaa agaaattata aaaaatagag accaagcaaa
      121 tcgtattgta gaacaagaac aagataaaaa attaatagca ttttttaaag aaactgtaaa
      181 attgaatgaa gttgaaaaaa caattgaaga gtttaatgct atgtttgaaa aaaaataaaa
      241 aatacagtca gttttataaa gggataaagt aatttttatc cctttttttg tgtctcatag
      301 ttacataatt agtaaaaatg agtatttttg aatgaattaa aaattacata tatgaatgat
      361 tttagaaaat atgcgaccaa acacttagga attagcagtg tgtcgctcga taaatacacg
      421 tcggttttaa ataattatat ttcacctaca attattgaag aacgtcagtt aaatatagct
      481 tcaatggata tattctctcg attgatgatg gatagaatta tattccttgg gttacctatt
      541 gacgattatg ttgcaaatat tgttcaaggt cagttattat acttagaatc tgcagattct
      601 tctcgtgata ttcaaattta tataaattct cccggaggtt ctgtgtatgc cggacttgga
      661 atatacgata ctatgcaata tattcaagca gatgtttcaa ctatttgtac cggaatggca
      721 gcatctatgg cagcagtatt gcttgcagga ggaactaaag gtaaacgatt agcattgaat
      781 cattcacgtg tgatgattca ccagccactt ggaggtgcac aaggtcaagc taccgatata
      841 gaaattacaa cacgcgaaat tttgaaattg aaacgcgaac tctacgaaat tatcgcccat
      901 catactggtc atgaatatgc aaaaattgaa aaagattcgg accgcgacta ttggatgaca
      961 tcgcatgaag ccaaagaata tggcatgata gacgatgtga tgatgcgaaa ataatatcac
     1021 atttcttccc tgttaggaag tatgaaacaa attttggtac atacatgctg cgcaccttgt
     1081 gcttcggcat cggtagaacg actgatagca aatggttggc agcctgtatt gtatttttat
     1141 atgcccaata tttttccgtt cgaagagttt gaaaaacggt tgtcctacgt tcaaaagctt
     1201 gcagattatt ataatattca attgatagta ggtgattaca atcactccaa ttggttggga
     1261 gcaatcaaag gaacagaatc tgcacccgaa ggcggtgctc gttgtgaaat ttgttataaa
     1321 actttgctcc aagccacttc aatcaaagcg caagagttgg aaattttaca tttctgcact
     1381 acgcttacta taagcccaca caaaaattcc aaaaaaatag aagaaatagg ttcgcaatat
     1441 caaggctata cacactacga tttcaaaaag caagacggat acaaacgaag cat
//
LOCUS       DAOTOW010000630         1492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_67120_length_1492_cov_3.034099, whole genome shotgun sequence.
ACCESSION   DAOTOW010000630 DAOTOW010000000
VERSION     DAOTOW010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67120_length_1492_cov_3.034099"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..411)
                     /locus_tag="P5243_09945"
     CDS             complement(43..411)
                     /locus_tag="P5243_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19815.1"
                     /translation="MDTKTSILYVDDEPINLMLFELNFKSKYHVLTANNAKEGLEILT
                     KNPHIPIVISDMKMPEINGVDFLRNVKENFPQCSCFILTGFDITPEIQKALRDHIILK
                     YLSKPFVIHEINTAIASALN"
     gene            complement(469..>1492)
                     /locus_tag="P5243_09950"
     CDS             complement(469..>1492)
                     /locus_tag="P5243_09950"
                     /inference="COORDINATES: protein motif:HMM:NF023809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HRS19816.1"
                     /translation="QKSSHGAQTFTFTPDINKTFSRQFTDYYIQKKQNTIANFDTPKS
                     QGEYIGRISKIEGKKICIETNVALRNGDGLCFLNSAGELSGVRVNSVTDNYIELTENV
                     NVPIGTRIWRNLSVQFEKQVEQSNHCRYIEASIQFQENQQGYTVFITDEYGYEAFVER
                     IIVKHPAVQVEKMKKSIQQQLSKTGQSNFKITHVSIDWHTPFFIQIAELNEARRLLIQ
                     QLQTVRLEAYVRIEKTIIPNAISFPLNVPIDARLNVSNVYAQAFYKRHGIHITPQAFE
                     LQKDNSIPLMTTKYCIRFETGNCPKQNNNIIAAEPIHLKDNKHRYRVEFNCKRCEMLI
                     FSETER"
BASE COUNT          465 a          224 c          204 g          599 t
ORIGIN      
        1 attaaaaaaa taaatttttt tattcgttat caataagcat aattaattca atgcacttgc
       61 aattgcagta tttatttcat gaattacaaa tggttttgac aaatatttga gtataatatg
      121 gtctcgcaat gctttttgaa tttcaggtgt tatatcaaaa ccggttagaa taaaacatga
      181 acactgtgga aaattttctt ttacatttcg caaaaaatct actccattta tttcgggcat
      241 tttcatatca ctgattacaa ttggaatatg aggattttta gtaagaattt ccaatccctc
      301 ttttgcattg tttgcagtta atacatgata ttttgattta aaattgagct caaacaacat
      361 aagatttatc ggctcatcgt ctacgtataa tatagatgtt tttgtatcca tatttcatca
      421 tggttttagt aatttcacta cacaaatata cacatatata cgcaactatc aacgttctgt
      481 ttcagaaaaa attaacattt cacatctttt acaattaaat tctactctgt acctatgttt
      541 attatctttc aaatgtattg gttctgctgc aattatattg ttattttgtt taggacaatt
      601 acctgtttca aaacgaatac aatattttgt agtcataaga ggaattgagt tatctttttg
      661 taattcaaat gcttgaggag ttatgtgtat tccgtgtctt ttataaaatg cttgtgcata
      721 tacattactt acatttaatc ttgcatctat aggtacattc aacggaaaag atattgcatt
      781 tggaataata gttttttcaa tacgaacata tgcttctaaa cgaactgttt gcaattgctg
      841 aatcaataat ctgcgagctt cgtttaattc tgcaatttga ataaaaaacg gagtatgcca
      901 atctattgat acatgtgtaa ttttaaagtt actttgacct gtctttgaga gttgctgctg
      961 tatagatttt ttcatttttt caacttgcac agcaggatgt tttactatta ttctttcaac
     1021 aaatgcttca taaccatatt catctgttat aaatactgta tatccttgtt gattttcttg
     1081 aaattgaatt gaagcttcaa tatatctaca atggttactt tgttctactt gtttttcaaa
     1141 ctgaaccgat agatttctcc atatacgagt tcctattgga acattgacgt tttctgttaa
     1201 ttctatataa ttatcggtaa ctgaattaac tcttacccca cttaattcac ctgcagaatt
     1261 taaaaaacat aagccatctc cattacgcag agctacattg gtttcaatac atattttttt
     1321 accttcaatt tttgaaattc ttcctatgta ttcaccttgc gatttaggtg tatcaaaatt
     1381 tgctatggtg ttttgttttt tttgaatata ataatcagta aattgtctgg aaaatgtttt
     1441 attaatatcg ggtgtaaacg taaaagtttg agcaccatgt gatgattttt gt
//
LOCUS       DAOTOW010000631         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_67709_length_1483_cov_2.635854, whole genome shotgun sequence.
ACCESSION   DAOTOW010000631 DAOTOW010000000
VERSION     DAOTOW010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67709_length_1483_cov_2.635854"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..>1483
                     /locus_tag="P5243_09955"
     CDS             394..>1483
                     /locus_tag="P5243_09955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS19817.1"
                     /translation="MKKSISLLLLLLPIVLQAQIATGYQVGTWYGFKKVAITYSFDDN
                     TSNQIPVAVPLLNKYNYKATFNTVVNWVGGNWSGWQTLANNGHEIASHTVTHATLPNI
                     SVSEQDTECKNSQSTIRTNTGSECVTITYPNCNIGDRTTLAKYYIAGRTCDGQTASNN
                     PSDFFTIGSIICGSQGAMKTASDFNTRISSAVASQGWCVFLIHGIDNDGGYSPLTSSE
                     FNSHLGYVNTNAATYWVAPFSTVVKYIKERNALSLTETAITADSLRVVATHNLTSTLT
                     TYNTPLTVRRELPSGWTGANVYKNSTKITSTIVTESGKTYVMFDVVPNDGTIFIAKSG
                     TTGGGGTTTFTELITNGEMDSGTTGWTVQ"
BASE COUNT          506 a          222 c          253 g          502 t
ORIGIN      
        1 gtattgctac tcgttttatt attacgcgag aattatctaa tgattttgtt cctataaata
       61 ttgaactgtt tttaccgtgt ttgttcggta tgtgtatagt ggtattttca tcgattgaat
      121 atgaatcaaa taatccttta aagagcatgt ttttattaca aaaatgtaat gaatattatt
      181 ccataaaaat actgtgtttt tttgaaatat ttatatttct tattttttta aaatacataa
      241 aatacagcat ttcaataatt tattatttta ttatactaaa aaatgaatga atttctatag
      301 tatttgaata aattttataa tggttataca aatacttatt gtaattttaa cacttggatg
      361 ggatacatac aataattatt aatttttaaa acaatgaaaa agagtatatc tttattattg
      421 ctattgttgc caatagtatt acaagcacaa atagcaactg gttatcaagt aggaacttgg
      481 tatggattca aaaaagttgc catcacatat tcattcgatg acaataccag taatcaaata
      541 ccggtagcag taccgttatt gaataaatat aactataaag ctacgtttaa tacggtagta
      601 aactgggttg gaggtaattg gagtggttgg caaacccttg ctaataatgg tcacgaaatt
      661 gcctcgcata cggtaacgca tgctacattg ccaaatattt ctgtttcgga acaagatacc
      721 gaatgcaaaa attcacaatc aactattcgc actaataccg gttcagagtg tgttactata
      781 acatatccta attgtaatat tggtgataga acaacattgg ccaaatatta tatagcagga
      841 agaacgtgtg atggtcaaac agcttcaaat aatccatcgg atttttttac aattggttct
      901 attatttgtg gttcgcaagg agctatgaaa acagcttctg attttaacac tcgtatttca
      961 agcgcggttg catcgcaagg ttggtgtgtt tttttaatac atggaataga caatgatggt
     1021 ggctattctc ctttaacttc atctgaattt aattcgcatt tgggatatgt aaatactaat
     1081 gcggctacgt attgggttgc accgttttct acagtagtaa aatacattaa agaacgtaat
     1141 gccctatctc ttacagaaac agcaattaca gctgattcat tacgggtggt agcaacacat
     1201 aatcttacaa gtactcttac tacgtacaat acgcctttaa ctgttcgacg tgaactccca
     1261 agtggttgga caggagcaaa tgtgtataaa aattctacaa aaattacttc aactatagtt
     1321 actgaaagcg gcaaaaccta tgtaatgttt gatgtagtac caaatgatgg aactatattt
     1381 atagctaaat caggtactac cggtggaggt ggcacaacta catttacaga attaataaca
     1441 aatggagaaa tggattcggg tactaccggt tggacagtac aaa
//
LOCUS       DAOTOW010000632         1482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_67766_length_1482_cov_3.377715, whole genome shotgun sequence.
ACCESSION   DAOTOW010000632 DAOTOW010000000
VERSION     DAOTOW010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67766_length_1482_cov_3.377715"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="P5243_09960"
     CDS             <1..458
                     /locus_tag="P5243_09960"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRS19818.1"
                     /translation="SFDILYVQRTQNLHDKHSGQISFPGGGYSKQDSSLCDTAVRETF
                     EEIGVLVNSNCIVGKLSEHFVPISNYIIHPYVAILSNAPHTFILQTSEIERVIAVPIE
                     FVQDTSHIRTKQVEYQNTIVTIPYYAWEQFEIWGVTAMITEEFVECILR"
     gene            complement(463..>1482)
                     /locus_tag="P5243_09965"
     CDS             complement(463..>1482)
                     /locus_tag="P5243_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRS19819.1"
                     /translation="LEKLVNKLCPKVKTIVGHGQMEGEKLEQIMLDFVNGEYDVLLAT
                     TIIESGLDIPNANTIIVNDAHHFGLSDLHQLRGRVGRSNKKAYCYLLAPPKHTLTKEA
                     RQRLYAIEEFSDLGSGFNIAMQDLDIRGAGNLLGAEQSGFISDIGYETYQKILDEALL
                     ELKETEYKELFSPESDNEQITINRLQDMTFVSDCVVETDLELLFPDWYIESTKERVKM
                     YRELDNIETEEDLELFKEQLRDRFGIIPPQSIELIEIVRLRKKAIALGIEKIILKNAT
                     LLLYFISNQDSAYYQSSVFGNVLQYVQQNPTICNMKESNGKLTLRFKNIHSITHAIEL
                     IECIV"
BASE COUNT          458 a          246 c          230 g          548 t
ORIGIN      
        1 attcatttga tatactatat gttcagcgaa ctcaaaattt acatgataaa cacagtgggc
       61 aaatttcgtt ccccggagga ggttattcta agcaagatag ttctttgtgt gatactgctg
      121 tgagagaaac gtttgaagaa ataggagtgc tcgtgaatag taattgtatt gttggtaaat
      181 tatctgaaca ttttgttcct attagtaatt atattataca tccgtatgtt gccattttat
      241 caaatgctcc acatacattt atattacaaa catctgaaat tgaacgtgta atagctgttc
      301 ctatagagtt tgtgcaagat acatcacaca taagaaccaa acaagttgaa tatcaaaata
      361 ccatagttac aattccatat tatgcatggg aacaatttga aatttgggga gttacagcta
      421 tgattaccga agaatttgta gaatgtatat tacggtaaag ttttaaacaa tacattctat
      481 taattcaata gcatgagtta ttgaatgaat atttttgaaa cgcaaagtaa gttttccgtt
      541 agattctttc atattacaaa ttgttgggtt ttgctgaaca tattgtaaaa cattaccaaa
      601 tactgaagat tgatagtatg cagaatcttg atttgatata aaatacagga gcaaagttgc
      661 atttttaaga ataatttttt caattcctaa tgcaattgct tttttacgta atcgtacaat
      721 ttctattaat tcaatacttt gcggaggaat tattccaaat ctgtcacgta attgttcttt
      781 aaatagttcc aaatcttctt cggtttctat gttatcaagt tctctataca ttttaacccg
      841 ttctttggtg ctttctatat accaatcggg gaataataat tctaaatcag tttcaacaac
      901 gcaatcactt acaaaggtca tatcttgtag tctgtttatt gtaatctgtt cattgtctga
      961 ttcaggacta aataattctt tatattcggt ttctttaagt tctaataatg cttcatctaa
     1021 tattttttgg tatgtttcat accctatgtc gctaataaat ccactttgtt cggctcccaa
     1081 taaatttccg gcacctcgta tatctaaatc ttgcattgca atattgaatc cactgcccaa
     1141 atcagaaaat tcttcaatag catataatct ttgtcgtgct tctttagtaa gtgtatgttt
     1201 agggggtgcc aaaaggtaac aatatgcttt tttgttactt ctgcctactc ttccacggag
     1261 ctggtgaagg tcgcttaatc caaaatgatg cgcatcgttt actattatgg tattggcatt
     1321 gggaatatca agccccgatt ctataattgt agtagcaagt aatacatcgt attctccatt
     1381 tacaaaatca agcataattt gctccaattt ttcaccttcc atttgtccat gacctacaat
     1441 agttttcact ttcgggcaca atttgtttac aagtttttct ag
//
LOCUS       DAOTOW010000633         1482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_67772_length_1482_cov_2.868956, whole genome shotgun sequence.
ACCESSION   DAOTOW010000633 DAOTOW010000000
VERSION     DAOTOW010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67772_length_1482_cov_2.868956"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="P5243_09970"
     CDS             <1..502
                     /locus_tag="P5243_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRS19820.1"
                     /translation="RDIISGLFIILENQYRVGDVIRIDNTGGMVENISLRMTTLRDNS
                     GIVHHIPHGDIKRVSNLSKDFSRINLDVAVSYNTNIEKAISVINAVGFELSKDPEWKE
                     RIITPPQFIRLDNFADSALILKINCDVQPLKQWEVSGEMRKRLKFAFDKEGIQIPFPQ
                     IVVHNA"
     gene            725..1336
                     /locus_tag="P5243_09975"
     CDS             725..1336
                     /locus_tag="P5243_09975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS19821.1"
                     /translation="MKIEKNNVVSLTYTLRKDNNSGEILEECTTARPLEFVFESGIML
                     PHFEKNIAGLESGNKFEFTLSPDEAYGTVNESAIVDVSKDIFIIDGVLREDLLQIGKI
                     IPMRDNSGNPLNGKIVEVNNTENKVIIDFNHPLAGTTLYFTGTIESIREATADELAYG
                     LHGQGGCGCGSGGCGSGSCSDDDDCCGSGNSGGCGSGSCGCGH"
BASE COUNT          519 a          202 c          251 g          510 t
ORIGIN      
        1 tcgcgatatt atctcggggc tatttataat attagaaaat cagtatcgtg taggtgatgt
       61 aattcgcata gacaataccg gaggaatggt tgaaaatatt tcgttacgca tgactaccct
      121 gcgtgataat tccggcattg ttcatcatat acctcatggt gatattaaac gtgtttctaa
      181 tttatcaaaa gacttttcac gtatcaattt agacgtagcg gtatcatata atactaatat
      241 agaaaaagct atttcggtaa tcaatgctgt aggatttgaa ttgtcaaaag accccgaatg
      301 gaaagaacgc attattactc ctcctcaatt tatacgatta gataattttg ctgattctgc
      361 acttattctc aaaataaatt gtgatgtaca accactcaaa caatgggaag taagcggcga
      421 aatgcgaaaa cgtttaaagt ttgcatttga taaagaaggt attcaaatac cttttcctca
      481 aattgttgtg cataatgcat aacaaaatat ctaaataatt caaattattt gtttttgttt
      541 tttacatatt acaaaatgat ttttacttaa aaaaaatact atacaagtac tatttttttt
      601 aattaagaaa ataatataaa aactctaaac agtacttcat aaaaaataat acatttgcac
      661 gtattcatgt aatgtttata aaacttatat gtctatttat tataaataaa aacattgtag
      721 tataatgaaa atagaaaaaa ataatgttgt atctctcacg tatactttgc gtaaagacaa
      781 caattcagga gaaattttag aagaatgtac aactgctcgt ccacttgaat ttgtttttga
      841 aagtggtata atgcttcctc attttgaaaa aaacattgca ggtttagaat caggtaataa
      901 atttgaattt actcttagtc ctgatgaagc atatggaact gtaaacgaat ctgctatagt
      961 agatgtaagt aaagatattt tcataattga tggcgtgtta cgtgaagatt tattgcaaat
     1021 tggaaaaata attccaatga gagataattc aggtaatcca ctcaatggta aaattgtaga
     1081 agtaaataat accgaaaata aagttataat agactttaat catcctttag caggtactac
     1141 tctctacttt acaggaacaa tagaatctat tcgcgaagct acagcagatg aacttgctta
     1201 cggattacat ggtcaaggcg gttgtggttg cggtagtggc ggatgtggta gcggcagttg
     1261 ctccgatgat gatgattgtt gcggatcagg aaactcagga ggttgtggta gtggtagttg
     1321 tggatgcggg cactaaacct atacttttac aatattttat ttttgtttat cttcatagaa
     1381 ttaaaaatac caatcgctat aattgatgat gtatcatttt tgaatacttt aattgttgtt
     1441 caatttatgt aaatctaaat ttcatgtaca gtattttttt at
//
LOCUS       DAOTOW010000634         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_68087_length_1478_cov_2.566409, whole genome shotgun sequence.
ACCESSION   DAOTOW010000634 DAOTOW010000000
VERSION     DAOTOW010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68087_length_1478_cov_2.566409"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="P5243_09980"
     CDS             <1..877
                     /locus_tag="P5243_09980"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS19822.1"
                     /translation="KKIIDLYTSEGLYFYLANDYKNASIYFSKVGNLIALKYDNLHDT
                     VRASTLIDCGTVARMGQNYDDAIKFYKLAIEMSPNPKIATYYDIVVCHKERKDTTAAI
                     QSLIQVIEKYPNDENIVGFTTELIQFYISLNQYDKALTYLTKALEKEPNNTNFLFNIA
                     YLYETEGKFDLAVENYKKAIEINPKDEGANLNLGIMYRNKAKKKLDEAEAAYGTKNYT
                     PLFNEGKSLLKLALPYLETYAQILEEKNPTDEKEKAVNNKLKKDAYKDIASIYAQLGM
                     NEKYNAAKKKMDSIK"
     gene            1091..>1478
                     /locus_tag="P5243_09985"
     CDS             1091..>1478
                     /locus_tag="P5243_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19823.1"
                     /translation="MKKIVLKEFKQKTKGGVYIRDNRYSLYFGSERFIYFNNKKSMEK
                     FVADTNRLLNDSLYNLNMLYSQVYAEYREYWFIQEKNTIEHQLLEIFQRIFTVFNRCA
                     YRTMEINVYSFRNLLCICDDLLHACNM"
BASE COUNT          610 a          178 c          221 g          469 t
ORIGIN      
        1 caaaaaaata attgatttat atacaagtga aggtttatat ttttatttag ctaatgatta
       61 taaaaatgct tcaatttatt tttcaaaagt tggtaattta atagcattaa agtatgataa
      121 tttgcatgat acagtaagag cttctacatt aatagattgt ggtacagttg ctcgtatggg
      181 acaaaattat gatgacgcta taaaattcta caaattagca attgaaatgt caccaaatcc
      241 taaaattgca acatattatg atattgtagt ttgtcataaa gaacgtaaag acacaactgc
      301 agcaattcaa tcattaatac aagttattga aaaatatccg aatgatgaaa atatagtggg
      361 atttacaaca gaattaatac aattctatat ttcattaaat caatatgata aagcattgac
      421 ttatttaact aaagcattag aaaaagaacc aaataataca aattttttgt ttaatattgc
      481 ttatttgtac gaaaccgaag gaaaatttga tttagctgtt gaaaattata aaaaagctat
      541 tgaaataaat ccaaaagacg aaggtgctaa tcttaactta ggaattatgt atagaaataa
      601 agctaagaaa aaattagatg aagccgaagc agcgtatggt acaaaaaatt atacaccatt
      661 atttaatgag ggtaaatctt tattaaaatt ggcgttacca tatttagaaa catatgcaca
      721 aattcttgaa gaaaaaaatc caactgacga aaaagaaaaa gcagtgaata acaaactaaa
      781 aaaagatgct tataaagata tagcatcaat atatgcacaa cttggtatga atgaaaaata
      841 taatgcagcc aaaaagaaaa tggattctat aaaatagcaa taaggggaac acgtaatggt
      901 tcccctttat aatcttatat tctatttaac ataatattaa ttataggaca aattaaaata
      961 tgtaatttgt gtaaattgtt gataatgtga tatgtttaaa attgtgaatt tggcagatta
     1021 ctcgtgtaat tcgcttttta aactgtaagt gtttgataat gaatatataa taaatataaa
     1081 tagtaacgct ttgaaaaaga ttgttttaaa agaatttaag caaaaaacca aaggtggcgt
     1141 gtatattcgc gacaacagat acagcctgta ttttggatca gaacggttta tttattttaa
     1201 caataaaaaa agtatggaaa aatttgtagc tgataccaac cggctactca atgattcact
     1261 atacaacctt aatatgctct attcacaagt atatgcagaa tatcgcgaat actggtttat
     1321 ccaagaaaaa aatactattg aacatcaatt gttggaaata ttccaacgaa tatttaccgt
     1381 atttaaccga tgtgcatacc gaactatgga aattaacgtg tatagcttta gaaatttact
     1441 gtgtatatgc gatgacctat tacatgcatg caatatgt
//
LOCUS       DAOTOW010000635         1477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_68144_length_1477_cov_3.218003, whole genome shotgun sequence.
ACCESSION   DAOTOW010000635 DAOTOW010000000
VERSION     DAOTOW010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68144_length_1477_cov_3.218003"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1477
                     /gene="pbpC"
                     /locus_tag="P5243_09990"
     CDS             <1..>1477
                     /gene="pbpC"
                     /locus_tag="P5243_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRS19824.1"
                     /translation="IVLFEDEYFFSHPGINPVSMWRALRQNIRNKRITSGGSTISMQV
                     IRLSRNGTRRTIFEKIYEILLAIRLELSYSKQEILNMYVSHAPFGGNVVGLEAASWRY
                     FNRPAHMLSWGEAASLAVLPNAPALIFPGKNEHLLLKKRNKLLHKLYSKGIIDKTTLD
                     LAQLEIMPQKPQPLPNYALHLLNKAINDGNEGKKLQTTIHYNTQKHIQTIAQSYAYKY
                     ASNYIHNIAILVTDTKTGNILAYIGNVESPRQTEVYVDNCISKRSSGSILKPILYANM
                     LNNGTLLPNSLVSDIPTRIGSYAPQNFEKTYEGVVPASEALAKSLNIPAVRMLQEYGV
                     APFLYSLQKIGFNSIQKSADYYGLSLILGGAEVSLWEAVSVYASMARALITYNTNKTY
                     CNSDYHSIQYSATTPQNKEYTNDALIGAGALYYTFKSLSMLTRPWGEIGWEQFASAHT
                     IAWKTGTSFGHRDAWAIGVTPEYTVGVWVGNSTGEGRPGLTG"
BASE COUNT          513 a          304 c          289 g          371 t
ORIGIN      
        1 attgtactct tcgaagatga atactttttt tcgcacccgg gcataaatcc ggtatcgatg
       61 tggcgagcat tgcgacaaaa cattcgcaac aaacgaatta caagcggagg aagtactata
      121 agcatgcaag taattcgttt gagtagaaac ggaacaaggc gaacaatttt tgaaaaaata
      181 tatgaaatac tacttgctat tcggttagaa ttatcgtact caaaacaaga aatactgaat
      241 atgtatgtat cgcatgctcc gtttggcggt aatgtggtag ggctcgaagc tgcatcgtgg
      301 cgatatttta accgtcctgc ccacatgctc tcgtggggcg aagctgcatc gttggcagta
      361 cttcccaatg ctccggcatt aatttttcca ggcaaaaacg aacatctgtt actcaaaaaa
      421 cgaaataaac tattacacaa actctatagt aaaggaatta ttgataaaac aactcttgat
      481 ttggcacaat tagaaattat gccgcaaaaa ccacaaccac tgccaaacta tgctctgcat
      541 ttgctcaaca aagctataaa cgacggaaac gaaggcaaaa aactgcaaac aacaattcat
      601 tataatacac aaaaacacat acaaactata gctcaatcat atgcatacaa atatgcatca
      661 aactatatac acaacattgc aatactggta accgatacca aaaccggaaa tattttggca
      721 tacataggca acgtagaatc gcctcggcaa acagaagtat atgtagacaa ctgtatttca
      781 aaacgtagtt cgggaagtat attaaaacca atattgtatg ccaatatgct caacaatggt
      841 acactattgc caaactcatt agtgagcgat attcctacac gtattgggag ttacgctccg
      901 caaaattttg aaaaaacata cgaaggtgtt gttcctgcat cagaagcatt ggcaaaatcg
      961 ctcaacatac cggcagtgcg tatgttgcaa gaatatggtg tagctccgtt tttgtattca
     1021 ttacaaaaaa ttgggttcaa cagcattcaa aaatctgccg actactacgg attgtcgctt
     1081 atattaggag gagccgaagt aagtttatgg gaagcagtgt cggtatatgc atcaatggca
     1141 cgagctctta taacatataa caccaacaaa acatattgca atagcgacta tcatagcata
     1201 caatatagtg ccacaacacc acaaaacaaa gaatatacaa acgatgcact tataggtgca
     1261 ggagcactgt actacacctt taaatcattg agcatgctca cccgtccttg gggtgaaatt
     1321 ggttgggaac aatttgcttc ggcacatacc atagcatgga aaacaggaac cagctttggc
     1381 catagagatg catgggctat aggtgttaca cccgaataca ctgttggggt atgggttggc
     1441 aattctaccg gcgaaggcag accgggactt accggat
//
LOCUS       DAOTOW010000636         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_68209_length_1476_cov_3.554539, whole genome shotgun sequence.
ACCESSION   DAOTOW010000636 DAOTOW010000000
VERSION     DAOTOW010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68209_length_1476_cov_3.554539"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="P5243_09995"
     CDS             complement(<1..1450)
                     /locus_tag="P5243_09995"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRS19825.1"
                     /translation="MKEKNATSIKTIEFIDQQLTSVVDSLSYAEKNLETFRSANKIVN
                     LSEEGSTMFKKLEELQAKKSLIDIKIKYYEYLLKTIRDKSNFKDIITPSIIGVQDQLL
                     NSLVSQLSQLYSERQVLEYTATGDNPSLQIINMKIKNTIAALIDNVNNLIESAQIESK
                     ETQKEIAKVDSEIKKLPSTERELINIQRKFELNDNIYNYLLQKRAEVGITKAANLPDL
                     KFIDYALLDNTLLKSPNRKLNYLIALFLGFFLPGLLLIVRDFMNDTIVEREEVERKSN
                     IPILGSVAHNKRINEVLPVKIHPKSSIAESFRAIRTNLSFFINHKSTKHPIISFTSTI
                     SGEGKTFCALNLATIIAMNNKKVLVVGLDLRKPKLHTMLKTNISYGITNYLIGTHQIE
                     DILIHTDIQNLDVILSGPIPPNPLELIESEKFAEFVSYIKNLGYDYILFDTPPIGLVA
                     DTLSIAKYTDLNVFVMRQNYTNRNAIKFINDIA"
BASE COUNT          485 a          221 c          196 g          574 t
ORIGIN      
        1 tagctatatc gtttataaac ttaattgcat ttctatttgt ataattttga cgcattacaa
       61 atacatttaa atcagtatac tttgcaatag ataatgtatc tgcaactaat cctattggcg
      121 gagtatcaaa taggatataa tcatatccta aattttttat gtatgaaaca aactcagcaa
      181 atttttcact ttctatcaat tctaatggat ttggtggtat aggaccggat aatataacat
      241 ctaaattttg aatatcggta tgtattaaaa tatcttctat ttgatgtgtt cctattaaat
      301 aatttgtaat accatatgaa atatttgttt tcaacatagt atgtaatttg ggttttctaa
      361 gatctaatcc tacaaccaaa acttttttat tattcatggc aataattgta gctaaattaa
      421 gtgcacaaaa tgtttttcct tcgccactaa tagttgaagt aaatgatata ataggatgtt
      481 tagtactttt atgattgata aaaaaagata aattggtacg aattgctctg aatgattctg
      541 caatagatga tttaggatga attttcacag gcaacacctc gtttatgcgt ttattgtggg
      601 ctacagaacc taatataggt atattcgatt ttctttctac ctcttcacgt tctacaatag
      661 tatcgttcat aaaatcgcga actattaaca ataacccggg taaaaagaat cctaaaaaca
      721 aagcaataag ataattcaac ttacgattgg gtgattttag caaagtatta tcaagcaaag
      781 catagtcaat aaactttaag tctggcaaat ttgctgcttt ggtaattcca acttctgcac
      841 gcttttgtaa taaataatta taaatattat cgttcaactc aaacttacgc tgaatattta
      901 taagctctct ttcggtactt ggtaattttt tgatttctga atctactttt gctatttctt
      961 tttgcgtttc ttttgattct atctgagccg attctattaa attatttaca ttatctatta
     1021 aagctgcaat agtattttta attttcatat ttataatctg caaactcgga ttatctccgg
     1081 tagcagtata ttcaagtacc tgacgttcag aatacaactg actcaactga cttaccaaag
     1141 aatttaacaa ttgatcttga actccaataa tagaaggcgt aattatatct ttaaaattac
     1201 ttttgtctct aatggttttt aataaatatt cgtaatactt tattttaata tcaatgagtg
     1261 atttctttgc ttgcaattct tctaactttt taaacattgt agaaccttct tcactcagat
     1321 ttacaatttt attggcagaa cgaaatgttt caagattttt ttcggcatac gataaagaat
     1381 caacaacact ggtaagctgt tggtctatga attcaattgt tttaatagat gtagcatttt
     1441 tttctttcaa atcattttca atagcttcta ttatta
//
LOCUS       DAOTOW010000637         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_68244_length_1476_cov_2.173821, whole genome shotgun sequence.
ACCESSION   DAOTOW010000637 DAOTOW010000000
VERSION     DAOTOW010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68244_length_1476_cov_2.173821"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="P5243_10000"
     CDS             complement(<1..375)
                     /locus_tag="P5243_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRS19826.1"
                     /translation="MCSHTASETSLYQDSLIVEGARVHNLKNISVEIPRNALCVITGL
                     SGSGKSSLAFDTIYAEGQRRYIETFSAYARQFLGNLERPDVDKITGLSPVISIEQKTV
                     NRNPRSTVGTITEIYDFFRLLYA"
     gene            complement(382..1311)
                     /locus_tag="P5243_10005"
     CDS             complement(382..1311)
                     /locus_tag="P5243_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRS19827.1"
                     /translation="MKSNIQKISYILLGVCISGILILFSFSDTPQIKKTDTQKETTFV
                     AVDLYIPDKISIFGESVPLEYYDVVESLERELLVNVYYQSQTVQYLKYVHRYFPIIDS
                     VLQANNLHTDFKYLALAESGLRVNISPKGAAGFWQFLETTAKEYNMTVNEQIDQRYDL
                     IKSTEAACKYLKTGYNKFNSWTMVAASYNLGMGGLNKQAEFQKVTSYYDLYTNSETAR
                     YVFRIIALKLIIENPEKYGYHEIKPYPSIPYTTVTVDSTIQNLQEFAISQGSNYKLLK
                     TMNPWLRDKYLPNPQRIPYKIIIIKNSDRILKK"
BASE COUNT          468 a          207 c          249 g          552 t
ORIGIN      
        1 agcatagagt aaacgaaaaa aatcatagat ttcggtaata gtaccaactg tagaacgagg
       61 attacggttt acagtttttt gttctataga aataaccgga ctcaatcctg ttattttatc
      121 aacatcaggt cgttctaaat taccgagaaa ttggcgagca tatgcagaaa aagtttcaat
      181 atatcgacgt tgtccttcgg cataaatggt atcaaatgca agtgatgatt tgccacttcc
      241 acttaatccg gtaattacac agagtgcatt tcgcggtatt tcaacagata tatttttaag
      301 attgtgtaca cgagcacctt ctactataag agaatcttga tataatgatg tttcagatgc
      361 agtatgagaa cacatgaagt attatttttt gagaattctg tcagaatttt tgataatgat
      421 aattttgtat ggaattcgtt gtggattagg taaatatttg tcgcgtaacc aagggttcat
      481 agtttttaac agtttgtaat ttgaaccttg tgatattgca aattcttgta aattttgtat
      541 tgtactatct accgtaacag ttgtatatgg aatagaagga tatggtttga tttcatgata
      601 tccgtatttt tcggggtttt ctattataag ttttaatgct ataattctaa aaacatagcg
      661 agctgtttct gaattggtat ataagtcata atatgatgta actttttgaa attctgcttg
      721 tttgtttaat ccccccatgc ctaaattata tgaggctgca accatagtcc acgaattgaa
      781 tttattatat cctgttttta aatatttaca cgcagcttcg gttgatttta taaggtcgta
      841 tcgttggtca atttgttcgt ttacggtcat attgtattct tttgctgtag tttctaaaaa
      901 ttgccaaaaa ccggcagctc ctttgggtga tatatttaca cgtagcccac tttctgcaag
      961 agccaaatat ttgaagtcgg tatgtaagtt atttgcttgt aatacactgt caattatagg
     1021 aaagtagcga tgtacgtatt ttaaatattg aactgtttgt gattgataat acacattaac
     1081 caatagttct ctttctaggc tttctactac atcataatat tctaatggta ccgattctcc
     1141 aaaaattgaa attttatcgg gaatatataa atctacagca acaaaagttg tttctttttg
     1201 tgtgtctgtt ttttttattt ggggagtatc agaaaatgag aacaatataa gtattcccga
     1261 tatgcataca cctaataaaa tgtagctgat tttttgtatg tttgatttca ttgctattat
     1321 ttattttttt gttttacaat acgcgacaat ataaagaaac aaatagaaat acttattcca
     1381 attgacgcta aaataagata atagttttta tgaattataa catttttaaa tgatatgata
     1441 tagaataccg cagcatttgt taatacagaa ataact
//
LOCUS       DAOTOW010000638         1473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_68462_length_1473_cov_1.858956, whole genome shotgun sequence.
ACCESSION   DAOTOW010000638 DAOTOW010000000
VERSION     DAOTOW010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68462_length_1473_cov_1.858956"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1285)
                     /gene="rffA"
                     /locus_tag="P5243_10010"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(161..1285)
                     /gene="rffA"
                     /locus_tag="P5243_10010"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRS19828.1"
                     /translation="MIPFNKPYLTGKELSYITEAVEVHGHISGNGVFTKRCQSFFEQR
                     WGFKKCLLTTSCTDALEMCALLLNIKPGDEVIMPSFTFVSTAIAFIRQGAVIKFVDSR
                     ADHPGMDEQLVEELITPKTKAIVVVHYAGVACDMDVIMDIANKHNLFVVEDAAQAIDS
                     YFTPPTSPQGEKRPLGSIGHLGCFSFHETKNIQCGEGGMLCINDDQFITRAEILWEKG
                     TNRAEFFRGEVNKYGWVDVGSSFLPSEINAAFLWAQLENLEDIQRRRLEIWNEYRYKI
                     LDIRYQFPVIPEYASNNAHMFYLVCSSLEERTEFITKMKKQEVNPVFHYQSLHASAFY
                     KDKHDGRVLPHADRYTDCLVRLPLFYEVDEKKILEIMVNY"
     gene            complement(1299..1424)
                     /locus_tag="P5243_10015"
     CDS             complement(1299..1424)
                     /locus_tag="P5243_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19829.1"
                     /translation="MSLAPAVVFIVGTVFLNEAFTLGKVLGLILIIAGIIVTVKL"
BASE COUNT          458 a          317 c          238 g          460 t
ORIGIN      
        1 acagggtcat aaaagtatgc atgtgtttta aagtattcat tactgatata gattgcacca
       61 acgcataata ctaccgaaaa aattatagaa attacaatga gcgaaatact atcttttttt
      121 gatgtgaata ttttcatact gtaactttat ccattaacaa ttaataatta accattattt
      181 caagtatttt cttctcatca acctcataaa acaaaggcaa ccgcaccaaa caatcggtat
      241 atctatctgc gtgtggtaat actctcccat catgtttgtc tttgtaaaac gcacttgcat
      301 gtaatgactg ataatgaaac accggattca cctcttgctt tttcatcttg gtaataaact
      361 ctgttcgttc ttctaacgat gagcagacta agtaaaacat gtgtgcatta ttacttgcat
      421 actctggtat tacagggaac tgatatctaa tatctaatat cttatatcta tactcattcc
      481 atatttcgag gcgacgacgc tgaatgtctt ctaaattttc taattgtgcc caaagaaatg
      541 cagcgtttat ttcggaaggc aaaaacgatg aaccaacatc gacccaacca tatttgttta
      601 cttcgccccg aaagaattcc gcccggtttg tccctttttc ccacagaatc tctgcacgtg
      661 taataaactg atcatcattt atacaaagca tgccaccttc gccacattgt atattcttgg
      721 tttcgtgaaa cgaaaaacaa ccgagatgcc ctatactacc taggggtctt ttctcccctt
      781 gaggggaggt aggaggggtg aaatacgaat ctattgcttg tgccgcatct tctactacaa
      841 ataaattgtg tttgtttgca atatccatta ttacatccat atcgcaggca acgccagcat
      901 aatgcaccac cactattgct tttgttttag gagtaattag ctcttctact aattgttcgt
      961 ccataccggg atggtcggca cgagaatcta caaatttgat taccgctcct tgccttataa
     1021 acgctattgc ggtagataca aacgtaaacg aaggcataat aacctcatcg ccgggcttaa
     1081 tattgagcaa caacgcacac atttccaaag catcggtaca tgatgtggta agcaaacatt
     1141 ttttgaaccc ccaacgttgt tcgaaaaagg attggcagcg ttttgtaaac actccgttgc
     1201 ccgatatatg cccatgaact tctacagctt cggtaatgta gctaagctct ttgccagtaa
     1261 gatagggttt attaaaggga atcattatta taactttttt acaacttaac cgttacaatt
     1321 ataccagcaa ttattaaaat taatccaagc acttttccta acgtaaacgc ttcgtttaaa
     1381 aacacggtgc ctactataaa tacaactgca ggagcaaggc tcataaatgg atacgctacc
     1441 gtaatttcaa attttgtcat agctatcatc caa
//
LOCUS       DAOTOW010000639         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_68494_length_1472_cov_3.335215, whole genome shotgun sequence.
ACCESSION   DAOTOW010000639 DAOTOW010000000
VERSION     DAOTOW010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68494_length_1472_cov_3.335215"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1472)
                     /locus_tag="P5243_10020"
     CDS             complement(<1..>1472)
                     /locus_tag="P5243_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19830.1"
                     /translation="DVMCFAIDVDGYVWSWGHGAWGQLTGIGQNLGHAYAKQVVGGET
                     GLPFLKAKAISAGQGFGMAVTLDGRAVAWGNNGATALSGGNLGDGTTNGSGQPVYIKT
                     SAGTPITNVATISDGDTWGFITRTDNTIWTWGDNSVGQLGIGSTTRQAYAVKINNPPT
                     CTTPDPIPFVSMPADFYTCLPFSYVIDPGFANLNNAYKYEWYKDGVLITGATSPTYTA
                     TQATTSGTTYKVKISYIGANSPCGMTPVEGDVKITAYVPEFTAPSGLTFCPPDFKVRV
                     NGSGTYDYYTAATGGSYLGTSYKNNFTTFPSANVTQIAGNVYTVYAQEAGNRAGMVKP
                     ITSTLTVQEDNAGHMQIKVFEDLTIDSIRFYAKPYYAPKASGGTIGAYQIGIYGQQIG
                     SNGPEPNTSQLLYSGTVKNLRSYSDSLPVLTLPVNITLPGSSGGEFYWIGFLQKAEPI
                     LEGPTGAYPYVDNVNGNLLQVIGFNKYGASTKEAAYSDIF"
BASE COUNT          442 a          316 c          235 g          479 t
ORIGIN      
        1 aaaatatatc tgaatatgca gcttcttttg tagaagcacc atatttatta aatcctataa
       61 cttgcaataa atttccgtta acattgtcaa catatgggta tgcacctgtt ggtccttcta
      121 atattggttc agctttttgt aaaaaaccaa tccaataaaa ttcaccacca gacgaacctg
      181 gcaacgtaat attaaccggc aatgtaagca ccggtaatga atccgaataa gaccttaaat
      241 tcttaaccgt tcctgaatat aacaattgtg aagtatttgg ttcaggtcca ttactaccaa
      301 tttgttgacc gtaaattcca atttgatatg ctccgatagt tccaccactt gcttttggag
      361 catagtaagg ttttgcataa aatcgaatac tatctattgt taaatcttca aaaactttaa
      421 tttgcatatg tccggcatta tcttcttgaa cagttaacgt actagtaatt ggctttacca
      481 tacctgctct atttcctgct tcttgagcat atacagtata tacgttgcct gctatttgag
      541 taacattggc cgaaggaaat gttgtgaaat tatttttata actagtacct aaatatgaac
      601 cacctgttgc agctgtataa taatcatatg ttccacttcc attaacacgt actttaaaat
      661 ccggaggaca gaatgttaat cctgatggtg ctgtaaattc aggcacatat gcggtaattt
      721 taacatcacc ttctaccgga gtcattccac acggtgagtt tgcgccaata tatgaaattt
      781 ttactttata tgtagtacct gatgttgttg cttgtgtagc agtatacgtt ggcgatgttg
      841 ctccggtaat aagaacacca tctttatacc actcatattt atatgcatta tttaaatttg
      901 caaatcccgg gtctataaca tatgaaaaag gtaaacacgt ataaaaatct gccggcattg
      961 acacaaaagg aattgggtcg ggagtagtac atgtgggcgg attatttatt tttactgcat
     1021 acgcttgacg tgttgtacta cctataccta attgtccaac actattatct ccccatgtcc
     1081 aaattgtatt atctgttctt gttataaatc cccacgtatc accatcagaa atagtggcaa
     1141 catttgtaat tggagtacct gcagaggttt taatatatac cggttgtcca ctgccatttg
     1201 tagtaccatc tcctaaatta ccaccactta aagcagtagc accattgttt ccccatgcaa
     1261 cagcacgtcc atcaagtgta actgccatac caaatccttg acctgctgaa atagcttttg
     1321 cttttaaaaa tggcaagccg gtttcacctc caactacttg tttcgcatat gcatgtccta
     1381 aattttgacc tataccggtt aattgccccc atgctccatg gccccacgac cacacatagc
     1441 catctacgtc aattgcaaaa cacattacat ca
//
LOCUS       DAOTOW010000640         1468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_68778_length_1468_cov_3.271054, whole genome shotgun sequence.
ACCESSION   DAOTOW010000640 DAOTOW010000000
VERSION     DAOTOW010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68778_length_1468_cov_3.271054"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="argS"
                     /locus_tag="P5243_10025"
     CDS             <1..742
                     /gene="argS"
                     /locus_tag="P5243_10025"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRS19831.1"
                     /translation="HIYVVGNEQNYHFQALTIVLKKLGFDWAEKIKHLSYGMVELPEG
                     KMKSREGTVVDADDLMESMLQDSIAISNELGKLSGLTPQEAAIVHETIAQGALKYFIL
                     KVEPRKTMLFNPKESIDFNGNTGPFIQYTYARISSVLRKAAQNNIDISAIVPHIECNE
                     KEREVLKQLSRYSEAIEIAGKEHAPSHIANYVYDLVKVYNQFYHDCSILNEEFADIRL
                     YRLQLSKQVACTIQSAMRLLGIQVPERM"
     gene            871..1098
                     /locus_tag="P5243_10030"
     CDS             871..1098
                     /locus_tag="P5243_10030"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HRS19832.1"
                     /translation="MAGFSFYNGVDVFEELKIGTELQLQAEENNKFDPYAVIVYYNGH
                     KLGYVPRQLNKESHKFLIMGYSDFLLQTLIV"
BASE COUNT          523 a          216 c          263 g          466 t
ORIGIN      
        1 gcatatttat gttgtaggga acgaacaaaa ctatcatttt caggcattaa ccattgtgct
       61 taaaaagctg ggcttcgact gggccgaaaa aattaaacac ctttcttatg gaatggttga
      121 actgcccgaa ggaaaaatga aatcgcgcga aggaaccgta gtagatgccg atgatttaat
      181 ggaaagcatg ttgcaagatt ctattgctat atcaaatgaa ttgggaaaac tttcgggttt
      241 aacgccgcaa gaagctgcaa tagtgcatga aactatagca caaggagcgt tgaaatactt
      301 tatattaaaa gtagaacctc gcaaaacaat gttgtttaac cccaaagaat ctatagattt
      361 taatgggaat accggaccat ttatacaata cacgtatgct cgtatttcct cggttttgcg
      421 taaagcagct caaaacaata ttgatatttc tgcaattgtg cctcatatag aatgtaatga
      481 gaaagaacgg gaagtgttaa aacaattatc gcgttattcc gaagcaatag aaatagcagg
      541 gaaagaacat gcgccttcac atattgctaa ttatgtatat gatttggtta aagtatataa
      601 tcaattttat catgattgta gtatattaaa tgaagagttt gcagatattc gattgtatcg
      661 attacaattg tcaaaacaag tggcatgtac tatacaatct gctatgagat tacttggtat
      721 acaagtaccc gaacgaatgt aatatttttt tataaatatt ttttctattg tcagaaattg
      781 acaactatag atagtttatt tgtaccataa tagttggtaa tattataaac aatagaaatc
      841 atagacaata gacatttaat taatttcagc attgcaggtt tttcattcta caatggagta
      901 gatgtgtttg aagaactaaa aatcggtaca gaattgcaac ttcaagccga agaaaataac
      961 aaatttgatc cgtatgcggt tattgtttat tacaatgggc ataaacttgg atatgttccg
     1021 cgtcagctaa acaaagaatc acacaaattt ttgataatgg gatatagtga ttttttatta
     1081 caaacattaa tcgtgtaagt cccgaagaac accccgaaaa tcaaatagga gtgttagtga
     1141 gaataaaaga gaaaagaaaa aaacgtgaat aaccaatcta tgttataatt agttttgagg
     1201 taggatgttg cttctataat tagattttca tcctgcctca atttttctaa aacaaaaaag
     1261 ggtaactgat tgctcaatta cccttggtat gtcggggcgg cagaacgcca accattctta
     1321 caaattattg ttatattgat agttatactt attaaatttt cgttggtcac gaaatggtca
     1381 cgaccttttc gctacttatg aataaataaa tacatttcaa tatgtaatga actcgattta
     1441 ttcaaaaata gacttttttc gttgattt
//
LOCUS       DAOTOW010000641         1464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69054_length_1464_cov_2.484741, whole genome shotgun sequence.
ACCESSION   DAOTOW010000641 DAOTOW010000000
VERSION     DAOTOW010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69054_length_1464_cov_2.484741"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="P5243_10035"
     CDS             complement(<1..419)
                     /locus_tag="P5243_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRS19833.1"
                     /translation="MENYYLLLVGILFLFAIVDVIVGVSNDAVNFLSSAIGAKAAPFY
                     VIMIVASLGVLAGAISSDGMMEIARSGIFIPSMFTFNNIMLIFVGVMITDVLLLDTFN
                     SLGLPTSTTVSIIFELLGGGVMMAIINLNNSGLPVSDV"
     gene            complement(514..>1464)
                     /locus_tag="P5243_10040"
     CDS             complement(514..>1464)
                     /locus_tag="P5243_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19834.1"
                     /translation="SASVKPFVPVGKIGTTVYSGFQTQTVGTEITQTGFILDRAYLGY
                     TYNPTESLSGEVKIDVGSPSDISDNDIELKRRFMYVRNAYGKYKINNTSILFGIINSN
                     QYKLQETTWGYRYVYKSFMDQHKFANSADIGISIEHTIADYLSVDASVCNGEGYTKLQ
                     SDMDFIYQLGAVFKPNKIVTFRLTGDYSNLQYKPVTIGSFLSIQPIEKLTLSAEYNIK
                     LNYEGKEEKNLTGSSIYANYNINEQFKVFARRDDLQSNTLDGQQNPWNYSKNGVAYIG
                     GIEYSPNKYLRIAANYQNWIFEDTSKETKQAFGILTELKF"
BASE COUNT          480 a          247 c          211 g          526 t
ORIGIN      
        1 acatcagaaa caggaagccc cgaattgttt agatttataa ttgccatcat tacaccacct
       61 ccaagcaatt caaatataat agaaactgtt gttgaggttg gtaaacctaa tgaattaaat
      121 gtgtctaaga gtaacacatc ggttatcata actcccacaa aaatgagcat tatgttgttg
      181 aaggtaaaca tgctgggaat gaatattccg gagcgtgcta tttccatcat gccgtcagat
      241 gatattgctc cagctaaaac ccctaatgat gcaacaatca ttataacata aaacggtgcc
      301 gctttggcac ctattgccga gcttaagaaa tttacagcat cgttacttac tcctactata
      361 acgtctacta ttgcaaataa aaataaaata ccaactaata aaagataata attctccata
      421 tatatacata aaaaagttta caatttctta acatacaaaa gtaaaaaaaa ggagttagta
      481 ataactccct ttttgtgtaa taagtttctt ttgttaaaat tttaattcag taaggattcc
      541 aaatgcttgt tttgtttctt tacttgtatc ttcaaaaatc caattttgat aatttgcagc
      601 aatgcgtaaa tatttgttag gagagtattc tataccgcca atatatgcaa caccattttt
      661 tgagtaattc catggatttt gttgtccatc taatgtattt gattgcaaat cgtcgcgacg
      721 agcaaatact ttaaattgtt cgttgatgtt gtaatttgca tatattgatg aaccggtaag
      781 atttttttct tctttgcctt cgtaattgag ttttatatta tattcggcac ttaatgttaa
      841 tttttctatt ggttgaatac ttaagaaact tccaatggta acaggtttgt attgtagatt
      901 tgaataatct cctgtcaacc taaatgtaac tattttatta ggtttaaata cagctcctaa
      961 ttgatatata aaatccatat cagactgtaa ttttgtgtag ccttctccat tgcaaaccga
     1021 agcgtcaaca gataaataat ctgctattgt atgttctata ctaataccta tatctgcaga
     1081 gtttgcaaat ttatgttgat ccataaacga cttgtacaca tatctatatc cccatgtagt
     1141 ttcttgtaat ttgtattgat tactgttaat aataccaaac aagattgaag tattattaat
     1201 tttatatttg ccgtatgcat tgcgtacata cataaatcta cgttttaatt ctatatcatt
     1261 atcactaata tcagaagggc ttccaacgtc aatttttact tcaccactta atgattcggt
     1321 aggattatag gtatatccta aatatgctct atctaaaata aaaccggttt gtgttatttc
     1381 tgttcctaca gtttgtgttt gaaatcctga atatacagta gtaccaattt ttcctaccgg
     1441 aacaaaaggt tttacgctgg cact
//
LOCUS       DAOTOW010000642         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69109_length_1463_cov_3.443892, whole genome shotgun sequence.
ACCESSION   DAOTOW010000642 DAOTOW010000000
VERSION     DAOTOW010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69109_length_1463_cov_3.443892"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..915
                     /locus_tag="P5243_10045"
     CDS             94..915
                     /locus_tag="P5243_10045"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRS19835.1"
                     /translation="MKNLILRFTTGILYIAIIVISLYTSPIVFYITFLLIHLAILHEF
                     YSLLHAKGIKTLSWLALSISALLFTASYCKHYWEIPISYHILLIPLFMLIFIIQLFKV
                     SKKPFHSIAYTILGLIYISFPFAILPNLAFDNNSNVLLSNIIMALFIFIWSNDTFAYV
                     WGMLFGKHLLFPRISPKKTVEGFIGGLISTIAIAIVISNYFFFDLTLYQWIGAAIVIV
                     IASTFGDLTESMLKRYLQIKDSGTILPGHGGFLDRFDSVIFSIPAFFTYLQIITL"
     gene            928..>1463
                     /locus_tag="P5243_10050"
     CDS             928..>1463
                     /locus_tag="P5243_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRS19836.1"
                     /translation="MKIHKEGYSTIVFTGAVLSLILLFLHQKNVESIILYPLSLVFLA
                     FFIFIISFFRNPKRVFTYNTNAIIAPADGKIVVIEEVEETEYLHCKCRQISIFMSPLN
                     VHINRYPCSGTVIESIHHSGKKLPAFNPKSSTENERTTIVMETHFGTIVFRQIAGAVA
                     RRIVNYAQKNDIANQNSE"
BASE COUNT          481 a          217 c          197 g          568 t
ORIGIN      
        1 acctagtaga actgcagata ttgaaaaaac tttagttttg ggagctcatg gaccgaaaga
       61 attaattgta ttattaatag aaaaatagca tatatgaaaa accttatttt acgttttaca
      121 acaggaattt tatatattgc tattattgta atttctcttt atacatcacc tattgttttt
      181 tatattactt ttttacttat acatcttgct attttacacg aattttattc tttattacat
      241 gcaaaaggaa ttaaaacttt atcttggtta gctctttcta tttctgcatt gctttttact
      301 gcatcatatt gtaaacatta ttgggaaata cctattagct atcatattct tcttattcct
      361 ttgtttatgt taatatttat aatacaattg tttaaagtat caaaaaaacc ctttcattct
      421 attgcatata ccatattagg actaatttat ataagttttc catttgctat attaccaaat
      481 cttgcatttg ataataattc caatgtatta ttatcaaata ttataatggc tttatttata
      541 tttatttggt caaatgatac atttgcttat gtgtggggta tgctgtttgg aaaacatttg
      601 ttatttccac gaatatcacc aaaaaaaaca gttgaaggct ttattggagg ccttatttct
      661 acaattgcta tagctatagt tatttcaaac tattttttct ttgatttaac tctttatcaa
      721 tggattggtg ctgcaatagt tattgttata gcatcaacgt ttggagactt aacagaatct
      781 atgctcaaac gatatcttca aattaaagat tcaggaacaa ttttgccggg acacggaggc
      841 tttttagaca gatttgatag tgtaattttt tcaattccgg catttttcac atatttgcaa
      901 ataattacat tgtaaattta tatagatatg aaaattcaca aagaaggcta ttcaaccata
      961 gtatttaccg gagcagtgtt aagtttgata ctattatttt tacatcaaaa aaatgtagaa
     1021 tcaattatat tgtatcctct ttcacttgtt tttttggcat tctttatatt tattattagt
     1081 ttttttagaa accctaaacg agtgtttaca tataatacaa atgcaattat agctcctgcc
     1141 gatggtaaaa ttgttgtgat tgaagaagtt gaagaaaccg aatatttaca ttgtaaatgt
     1201 cgtcaaatct caatttttat gtcaccactc aatgtacata taaacagata tccttgttct
     1261 ggcactgtca tagaaagcat tcatcattcg gggaaaaaac ttcctgcttt caatccaaaa
     1321 tcttctactg aaaatgaacg tacaaccatt gttatggaaa ctcattttgg aacaattgta
     1381 ttccgtcaaa ttgccggtgc tgttgccaga agaattgtaa actatgcaca aaaaaatgat
     1441 attgccaacc aaaattcaga att
//
LOCUS       DAOTOW010000643         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69110_length_1463_cov_3.433239, whole genome shotgun sequence.
ACCESSION   DAOTOW010000643 DAOTOW010000000
VERSION     DAOTOW010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69110_length_1463_cov_3.433239"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="P5243_10055"
     CDS             <1..260
                     /locus_tag="P5243_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRS19837.1"
                     /translation="GKEKTAYIWNNCSHQAAYNETGAQGVSYTTGVPAMVGAMMFLTG
                     KWTGTGVFNVEEFNPDPFMEVIGTYGLPWEIKEDIDLEFEY"
     gene            266..1402
                     /gene="nspC"
                     /locus_tag="P5243_10060"
     CDS             266..1402
                     /gene="nspC"
                     /locus_tag="P5243_10060"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRS19838.1"
                     /translation="MEDLYSKIPAPAFILNESLLRANLEKLAYVSKQADVQIILALKG
                     YALWKSFPLIRKYLHGATASSLNEVKLCNEYMGTRAHTYAPAYSKSEIKEIAEKSSHI
                     TFNSLNQYNAYKDICIKSCTRMGLRVNPGYSDITTDMYNPCSPNSRLGIPAHELQHGI
                     PDQITGLHFHALCENNSYSLERVLHAFEERFGHIIPQLQWINMGGGHLITQQDYNVEH
                     LISILKIFKQKYKAQIILEPGGAVGWQTGVLKSTVLDIVNYGNVPTAILDVSFAAHMP
                     DTLEMPYRPAIWGASKDPVDGYAAYRMGGQTCLAGDFLEAYYFPKELRIGDTIYLEDM
                     MHYTMVKTTMFNGVNHPAIYILKENGMVECAREFVYDDYKKRLS"
BASE COUNT          507 a          250 c          263 g          443 t
ORIGIN      
        1 acggcaaaga aaaaactgca tatatttgga ataactgtag tcatcaggca gcatataacg
       61 aaaccggtgc tcaaggtgtt tcatacacta ccggtgttcc ggctatggta ggagccatga
      121 tgtttcttac cggtaaatgg accggtaccg gcgtatttaa tgttgaagaa tttaatcctg
      181 atccttttat ggaagtaatc ggcacttatg gattaccttg ggaaataaaa gaagatatag
      241 atttagagtt tgaatattaa aaaacatgga agacttatat tccaaaattc cggctcctgc
      301 atttatttta aatgaatctt tacttagagc aaaccttgaa aaacttgcat atgttagcaa
      361 acaagctgat gtgcaaataa ttttggctct taaaggatat gcattgtgga aatcgttccc
      421 acttattcgc aaatatttac atggcgcaac tgcaagttca cttaacgaag taaagttatg
      481 taacgaatat atgggaacaa gagctcatac gtatgctcct gcatattcta agtcagaaat
      541 taaagaaatt gcagaaaaga gttcacatat tacctttaat tctctcaatc aatacaatgc
      601 ttacaaagat atttgcatta aaagttgtac tcgcatggga ttacgtgtaa atcccggtta
      661 tagcgatatt acaacagata tgtacaatcc gtgctctcct aactcaagac ttggtattcc
      721 tgcccatgaa ttacaacatg gaatacccga ccaaattaca ggattgcact ttcatgcttt
      781 atgcgaaaat aattcttatt cattggaacg tgttttacac gcatttgaag aacgttttgg
      841 acatataatt cctcaattac aatggataaa catgggaggc ggtcacttaa ttactcaaca
      901 agattacaat gtagaacatc ttatttctat tcttaaaatt tttaaacaaa aatataaagc
      961 tcaaattata ttagaaccag gtggtgctgt agggtggcaa acaggagtat taaaatcaac
     1021 cgtacttgat attgtaaact acggtaatgt acctacagct atactcgatg tttcttttgc
     1081 tgctcatatg cccgacactc ttgaaatgcc atacagacct gcaatttggg gggcaagtaa
     1141 agaccctgtt gatggatatg ctgcttaccg tatgggtgga caaacctgtt tagccggtga
     1201 ttttttagaa gcatattatt tcccaaaaga attacgcata ggcgatacta tatatttaga
     1261 agacatgatg cattatacaa tggttaaaac caccatgttt aatggggtaa atcatcctgc
     1321 tatatatatt cttaaagaaa acggtatggt ggaatgcgca cgagaatttg tatatgatga
     1381 ttacaaaaaa agattaagtt aaaaaaatac cttaatacta ttatataatg tacaattgaa
     1441 tcacaccatg caaaaacaag ata
//
LOCUS       DAOTOW010000644         1463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69129_length_1463_cov_2.622869, whole genome shotgun sequence.
ACCESSION   DAOTOW010000644 DAOTOW010000000
VERSION     DAOTOW010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69129_length_1463_cov_2.622869"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="P5243_10065"
     CDS             <1..673
                     /locus_tag="P5243_10065"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRS19839.1"
                     /translation="AFDNELPGAVLRVPDADGKRTEIVCATCKGHLGHVFEGERFTPT
                     NIRHCVNSVSLVFKPAALKTYDTAYFANGCFWGTQYWFNKAPGVIKTTVGYCGGHIEN
                     PTYKQVCAGNTGHAECIEVVFNTSKTDFRTLAKLFFNTHDPTQVNRQGPDIGEQYRSE
                     IFYTSALQKQIADELFVELYKKGYDIATEITAFSKFYPAEEYHQNYYEKKQGIPYCHI
                     FKEKF"
     gene            complement(670..1146)
                     /locus_tag="P5243_10070"
     CDS             complement(670..1146)
                     /locus_tag="P5243_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRS19840.1"
                     /translation="MCHITNKHTIYLLFCIALSIVPIQSCDKKENPVPFTSIQVTIFD
                     IESDPRYSALRVEGGSALLQRPSDCLGYKCNGVVIYRLKREYTYDDFRAFDVTCTHEA
                     NTCAMQLDSSFGDLLTCPCCGSIFTMQYGYMMQGPAKYPLREYVCTFDNGNLRIEN"
     gene            1203..>1463
                     /locus_tag="P5243_10075"
     CDS             1203..>1463
                     /locus_tag="P5243_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19841.1"
                     /translation="MSQKSILQILVITCLIWGVGNMQAFAQNKPKKGTYLPGKSPEQE
                     EKELQVKVEEETKKPDKPLTKRQGYKFMRKRDKRKEEEYKEHH"
BASE COUNT          546 a          236 c          265 g          416 t
ORIGIN      
        1 ggcatttgac aatgaattac ccggagctgt tttaagagtt cccgatgccg atggaaaacg
       61 taccgaaata gtttgtgcta cttgtaaagg gcatttgggg catgtgtttg aaggtgaaag
      121 gtttacacct accaatataa gacattgtgt aaattcagta tcactagtgt ttaaaccggc
      181 tgcattaaaa acatatgata ctgcatattt tgcaaacgga tgtttttggg gaactcagta
      241 ttggttcaac aaagctccgg gtgtaattaa aactactgta ggatattgcg gcggacatat
      301 tgaaaatcca acatacaaac aagtatgtgc aggtaacaca gggcacgctg aatgtattga
      361 agtggtattt aatacctcta aaactgattt tagaacactt gcaaaattgt tttttaatac
      421 acatgaccct acacaagtaa atagacaggg gcctgatatt ggcgaacaat acagaagtga
      481 aatattttat acctcggcac ttcaaaaaca aatagctgat gaattgtttg tagaattata
      541 taaaaaaggg tatgatatag ctacggaaat tacagctttt agtaaatttt atcctgccga
      601 agaatatcat caaaactact atgaaaaaaa acaaggcata ccgtattgtc atatattcaa
      661 agaaaaattt taattttcga tacgtaaatt accgttatca aacgtacaaa catactcacg
      721 taaggggtat tttgccggtc cttgcatcat atagccatat tgcatagtaa atattgaacc
      781 acaacaagga caggtaagta aatcgccaaa acttgaatca agttgcattg cacatgtatt
      841 ggcttcgtga gtacacgtaa catcaaatgc tctaaaatca tcataggtat attctcgttt
      901 gagtctgtat attaccacac cattgcattt gtaacccaaa caatccgaag gacgttgtaa
      961 tagagcagaa cctccttcaa ctcttaaagc actataacga gggtcgcttt ctatatcaaa
     1021 tatggtaact tgaatagagg taaagggtac cggattttct tttttgtcgc atgattgaat
     1081 tggaacaata ctcagagcta tacaaaataa caagtaaatt gtatgtttgt ttgtgatatg
     1141 acacataatg tatatatttg tataattaca aaagtactat aaattaatta ttcatacgtg
     1201 atatgtcgca aaaaagtata ttgcaaatac ttgtaataac atgccttata tggggagttg
     1261 gtaatatgca agcatttgct caaaataaac caaaaaaagg aacatatctt cccggcaaaa
     1321 gtcccgaaca agaagaaaaa gaattacaag taaaagtaga agaagaaact aaaaaacccg
     1381 ataaaccttt aactaagcga caagggtata aatttatgcg taaacgtgat aagcgtaaag
     1441 aagaagaata caaagaacat cat
//
LOCUS       DAOTOW010000645         1462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69199_length_1462_cov_2.970860, whole genome shotgun sequence.
ACCESSION   DAOTOW010000645 DAOTOW010000000
VERSION     DAOTOW010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69199_length_1462_cov_2.970860"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="P5243_10080"
     CDS             <1..583
                     /locus_tag="P5243_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19842.1"
                     /translation="AGKQIIKTISNNVMYKAEQTITFAGYGNNCIVKSGAITHVKASN
                     KITFLPGFKVEQGAHLHASIEPIAMNQASLLKSDNSQTNTINYSKPLKYSNIVSTYTN
                     NQIDSISICTDQPYIYPNPCKSILSITIPLENEYTQHSYLFSIFTPDGKIVKSGTLTQ
                     NANTIDVSDLAVGMYLIKIKHGDSIYIEKLLKN"
     gene            588..1286
                     /locus_tag="P5243_10085"
     CDS             588..1286
                     /locus_tag="P5243_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19843.1"
                     /translation="MRSIILCISVLFLICATSCRNKKGDFEITGTIINAGSKKPIDSV
                     MVILFGGNPSSTGIGAVDDLNDNPPNGNNDTTYTDKDGYFSVKIKKESYSFIGIYKEG
                     YRSPTYYTGNKVRGDNHFTVPGEYKVRIEYVAECSFYPIFMKIGNNSDIDTLRVFLDT
                     EYKNFPRNFPLAYAEKYVGISPFTYLNSRAYGFVGDTYVYYKLEYTDNGTWKTKIDSV
                     YIKSFETYTDTIYY"
BASE COUNT          552 a          198 c          226 g          486 t
ORIGIN      
        1 agctggcaaa caaataataa aaaccatttc taacaacgta atgtataaag cagagcaaac
       61 tattacgttt gcaggatatg gtaataattg tattgtaaaa tcgggtgcta taacacatgt
      121 taaagcaagt aataaaatta cgtttttacc aggtttcaaa gttgaacaag gtgctcattt
      181 acacgcatct attgaaccaa ttgctatgaa tcaagcttca cttttaaaat cagataattc
      241 acaaacgaat actattaatt attcgaaacc tttaaagtac agcaatatag tttcaacgta
      301 tactaataat cagattgaca gtatttctat atgtacagac caaccataca tatatccaaa
      361 cccatgtaaa tcaatactaa gcattacgat accgttggaa aacgaatata cacaacattc
      421 atatttattt tctatcttta cccctgatgg taaaattgtt aaatctggca cattaacaca
      481 aaatgcaaat acaatagatg tttcggattt agctgtagga atgtatttaa ttaaaataaa
      541 acatggtgat tctatttata ttgaaaaact tctaaaaaat taaatgtatg agatcaatta
      601 ttttatgtat atctgtttta tttttaatat gtgcaacatc atgcagaaat aaaaaaggtg
      661 attttgaaat tacaggaact ataatcaatg cgggttctaa aaaaccaata gatagtgtta
      721 tggtcattct ttttggcggt aatccttcct caaccggaat aggtgcagta gatgatttaa
      781 atgataatcc accaaatgga aataatgata ctacatatac agataaagat ggatattttt
      841 ctgtaaaaat taagaaagaa tcatattcat ttataggcat ttataaagaa ggatatagaa
      901 gcccaacata ttataccggt aataaagtac ggggagataa tcattttaca gttcctggag
      961 aatataaggt tagaattgaa tatgtggctg aatgttcttt ttatcccata tttatgaaaa
     1021 ttggaaataa ttctgatata gacacgctga gagttttttt agatactgaa tacaaaaatt
     1081 ttccaagaaa ttttccttta gcatatgcag aaaagtatgt tggcatttct ccatttacgt
     1141 atttaaattc aagagcatat ggttttgtag gtgatacgta tgtttattat aaacttgaat
     1201 ataccgacaa tggcacatgg aaaactaaaa ttgatagtgt gtatataaaa tcatttgaaa
     1261 catatacaga tacgatttat tattagtgag ttatgagaat aaaacatttt tttattgttc
     1321 caattgtagg atttttagtg ttatcatgca acaataaaaa aggtgatttt attgttacag
     1381 gaacagtaat aaatgcaggt tcaaaacaac caatagatag tgttatggtc attctttttg
     1441 gcggtaatcc ttcctcaacc gg
//
LOCUS       DAOTOW010000646         1461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69264_length_1461_cov_3.128734, whole genome shotgun sequence.
ACCESSION   DAOTOW010000646 DAOTOW010000000
VERSION     DAOTOW010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69264_length_1461_cov_3.128734"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1461
                     /locus_tag="P5243_10090"
     CDS             <1..>1461
                     /locus_tag="P5243_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRS19844.1"
                     /translation="LNNFGVNEIPALYLSKLGFQDTSTVISKLSGGQKKRVALAKILL
                     KNPDFLILDEPTNHLDLDVIEWLEDTLDSLQCTVLMVTHDRYFLDRVCNTIIEIDDKQ
                     AFKYTGNYSYFLEKKEERQILMSTEIAKAKNLMRTELEWIRRQPKARGTKAKYRIDAF
                     SDLKQKASKTIANKKLDITLQGSRLGNKVIELHGISKSYDSHRIIEKFSYLFHKKEKL
                     AIIGPNGCGKTTFLQLITNQLQPDSGYVEYGETVVVGYYKQDGLLLHEDCKVIEVIQN
                     ISETIEIENGNTISAAQFLEQWLFPRNMHYMYVSRLSGGEKKRLYLMTVLMKKPNVLI
                     LDEPTNDLDLFTLQILEEYLLHFSGCVIIVSHDRFFTDKVVDHLFIFKGNGIIEDFPG
                     NYTQFKIHEKILEDTLHKNEKIELQKSKVEKAIKGLTYKEKIELESIEKELEQLEQEK
                     KIIEQELIVGTLSNAELIVKSERIGKILQLIETNEL"
BASE COUNT          598 a          162 c          238 g          463 t
ORIGIN      
        1 tattaaataa ttttggtgtt aatgaaatac ctgcattata tctttcaaaa ttaggatttc
       61 aggatacgtc tactgtaatt tcaaaacttt ccggaggaca aaaaaaacgt gtggctttag
      121 caaaaatttt gttgaaaaat cctgattttt taattcttga tgaaccaaca aatcatcttg
      181 atttagatgt tattgaatgg ttagaagata cgttagattc tctgcaatgt acagtattaa
      241 tggttactca tgatagatat tttcttgata gggtttgtaa taccataatt gaaattgatg
      301 ataaacaagc ttttaaatat accggaaatt attcttattt tttagaaaaa aaggaagagc
      361 gtcaaatact catgtctact gaaattgcaa aagcaaaaaa tcttatgcga accgaactag
      421 aatggattcg tagacaacca aaagctcgtg gtacaaaagc aaaatataga attgatgcat
      481 ttagtgattt aaaacagaag gcaagtaaaa caatagcaaa taaaaagcta gacattacac
      541 tacaaggaag taggttagga aataaagtta ttgaattgca tggaatatca aaatcgtatg
      601 attcacatcg tattatagaa aaattttctt atttatttca taaaaaagaa aaactggcta
      661 taatagggcc aaatggttgt ggaaaaacaa cttttttgca attaattacc aatcaattac
      721 aaccggattc cgggtatgtt gaatacggtg aaactgttgt tgtaggatac tataaacaag
      781 atggtttatt attacatgaa gattgtaaag ttattgaagt tatacagaat atatccgaaa
      841 ctattgagat agaaaatggt aatacaattt ctgctgctca atttttagaa caatggttat
      901 tcccaagaaa tatgcattat atgtatgttt ctagattgag cggaggtgaa aaaaaacggt
      961 tatatttaat gactgtatta atgaaaaaac caaatgttct tattcttgat gaacctacta
     1021 atgatttgga tttgtttacg ttacaaatat tagaagaata tttacttcat ttttcgggat
     1081 gcgttataat agtttctcat gatagatttt ttacagataa agtagtagat catttattta
     1141 tttttaaagg aaatggcatt attgaagatt ttcccggaaa ttatactcaa tttaaaattc
     1201 atgaaaaaat actagaagat accttacata aaaatgaaaa gatagaatta caaaaatcga
     1261 aggtagaaaa agcaataaaa ggattaacgt ataaagaaaa aatagagtta gaatcaattg
     1321 aaaaagaatt agaacaatta gaacaagaaa aaaaaataat tgaacaagaa ttaatagtcg
     1381 gaacacttag taatgcagaa ttaattgtaa aatctgaacg aatagggaaa atattacaat
     1441 taattgaaac aaatgaatta c
//
LOCUS       DAOTOW010000647         1457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69546_length_1457_cov_3.918688, whole genome shotgun sequence.
ACCESSION   DAOTOW010000647 DAOTOW010000000
VERSION     DAOTOW010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69546_length_1457_cov_3.918688"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="P5243_10095"
     CDS             <1..1090
                     /locus_tag="P5243_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS19845.1"
                     /translation="ENKKWQDMLLEQKEPVTEENVAEVVAMMSGVPVKRIAQEEGARL
                     LKMYDELKGSVIGQDEAIKRIVKAIQRNRAGLKDPKKPIGSFIFLGPTGVGKTQLAKV
                     LAEYLFDSKDTLIRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSVVL
                     LDEIEKAHPDVFHILLQVLDEGHLTDSFGRKVDFKNTIIIMTSNIGTREITEFGNSIG
                     FSPKSKEEQVQEYAKSVVEKALKKAFTPEFLNRIDDLIQFNTLTKDNIYKIIEIELQD
                     LLNRVKHLKFNLHLTQELKDFIVEKGYDQRFGARPLKRAIQKYIEDPMAEFMLGNKIE
                     EGAYIQIDYKQDNVDISILPNAIVENIN"
     gene            1097..>1457
                     /locus_tag="P5243_10100"
     CDS             1097..>1457
                     /locus_tag="P5243_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19846.1"
                     /translation="MAIDSHSFLLHTPITLNLHETEIVLSDFIYNVHDIVDISTHSTI
                     EVWYNSKKHIVCLEIIDSQSLPQVSILEIVLQSGTKHHIALIKSKKIIHTIRFTGYAR
                     NVQIAGDFNSWNPKGFDF"
BASE COUNT          573 a          194 c          243 g          447 t
ORIGIN      
        1 agaaaataaa aaatggcaag atatgcttct tgaacaaaaa gaaccggtaa cagaagaaaa
       61 tgtagctgag gttgttgcta tgatgtcggg agtgccagta aaacgaattg ctcaagaaga
      121 aggtgctcga ttactgaaaa tgtacgatga attaaaaggc agcgtaattg gacaagatga
      181 agctataaaa cgaatagtta aagctataca aagaaataga gccggattaa aagatccaaa
      241 aaaaccaatt ggttcattta tatttttagg acctaccggt gttggaaaaa cacaattggc
      301 taaagtatta gccgaatatt tatttgactc taaagataca ttaatacgta ttgatatgag
      361 tgaatatatg gaaaaatttt cggtatcacg tttagttgga gctcctccgg gatatgtggg
      421 atatgaagaa ggtggacaat taacagaaaa agttcgcaga aaaccctact ctgtagtatt
      481 acttgacgaa attgaaaaag ctcatcctga tgtttttcat atattattac aagtattaga
      541 tgaaggtcat ttaactgata gttttggaag aaaagtagat tttaaaaata ctattattat
      601 aatgacgtct aatattggaa cacgcgaaat tacagagttt ggcaacagta taggatttag
      661 tccaaaatca aaagaagagc aagttcaaga atatgcaaaa tctgttgtag aaaaagctct
      721 caaaaaagca tttactcctg aatttttaaa tagaatagat gacttaatac aatttaacac
      781 tctcacaaaa gataacatct ataaaatcat tgaaatagaa ttacaagatt tacttaatag
      841 agtaaaacat ttaaaattca atttgcattt aactcaagaa cttaaagatt ttatagtaga
      901 aaaaggatac gatcaacgat ttggtgctcg cccactgaaa cgagcaattc agaaatatat
      961 agaagatcct atggctgaat ttatgcttgg taataaaata gaagaaggcg catatattca
     1021 aatagattat aaacaagaca atgtggatat atcaattttg ccaaatgcta ttgttgaaaa
     1081 tataaattaa atatgcatgg ctattgattc tcattcattt ttattacaca ctcctattac
     1141 actgaattta catgaaacag aaattgtttt aagtgatttc atttataatg ttcatgacat
     1201 tgtagatatt tctacacata gcactattga ggtttggtat aattcaaaaa aacacattgt
     1261 atgtcttgaa attattgatt cacaatcatt acctcaagtt tcaatattag aaatagtttt
     1321 acaatctggc accaaacatc acatagcttt aattaaatca aaaaaaataa ttcataccat
     1381 tcgttttaca ggatatgccc gtaatgttca aatagcaggt gattttaata gttggaatcc
     1441 taaaggtttt gatttta
//
LOCUS       DAOTOW010000648         1457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69553_length_1457_cov_3.102710, whole genome shotgun sequence.
ACCESSION   DAOTOW010000648 DAOTOW010000000
VERSION     DAOTOW010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69553_length_1457_cov_3.102710"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /gene="lpxK"
                     /locus_tag="P5243_10105"
     CDS             <1..985
                     /gene="lpxK"
                     /locus_tag="P5243_10105"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRS19847.1"
                     /translation="CITYLRNKAYDKGYIASIECKIPTICVGNITVGGTGKTPHVEYI
                     CNLLQNSMHIAILSRGYKRKTKGYILASNNTYVDEIGDEPYFLYKKLERTTVAVCENR
                     VVGVTQLLYDKPTIQAIILDDAFQHRALRAGLNIVLIDYNRPIWKDCVFPGGMLREGR
                     YALNRAHCIIVTKCPATISLEEQNMWKTKLRCTHQDVFFTKIVYRTIYEAETKEHKEI
                     PTILSSKHILLITGIAQPKPMYDFISEYCKNITHLNFADHHIYTKEDYDVILQNYKTY
                     RTTIVTTEKDAPKILEITRKQLPVFVLSIDIEFLNNTEPEFQNKIVDFVSQ"
     gene            982..1434
                     /locus_tag="P5243_10110"
     CDS             982..1434
                     /locus_tag="P5243_10110"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HRS19848.1"
                     /translation="MNSLAHTFLSGIDKQVIIGNFIGDFVKGQSYSQFPVGIQKGILL
                     HRSIDSFADNSPIVKKSKEFFKPLYGRYAGIIVDIVYDHFLSTHWHLYSAMRREYFIQ
                     YVYSCLCQYSCILPKRAQQLIPSIAYNNWMRYYASYYGLVLGCFFKTS"
BASE COUNT          544 a          236 c          225 g          452 t
ORIGIN      
        1 gtgtataacg tatctgcgaa ataaagcata cgacaaagga tatatagcat caattgaatg
       61 taaaattcca actatttgcg taggaaatat caccgtaggc ggcaccggca aaactcctca
      121 tgtagaatat atttgtaatc tattgcaaaa ttctatgcat atagccatat tgagcagggg
      181 atacaaaaga aaaacaaaag gatatattct tgcatcaaac aatacctatg ttgacgaaat
      241 tggcgatgaa ccttactttt tatataaaaa attagaaaga actaccgttg ccgtttgcga
      301 aaatagagtt gtgggggtta ctcaattatt atatgacaaa ccaacaatac aagcaattat
      361 attagacgat gcttttcaac atagagcttt acgtgccgga ctcaatatag tactaataga
      421 ctacaacaga cctatttgga aagattgtgt gttcccgggt ggaatgctgc gcgaaggaag
      481 atatgctctc aacagagctc attgcataat tgtaacaaaa tgccctgcaa ctatttcttt
      541 agaagaacaa aatatgtgga aaactaaatt acgatgcaca catcaagatg tatttttcac
      601 aaaaattgtt tacagaacaa tatatgaagc cgaaacaaaa gaacataaag aaattcctac
      661 cattctttcg agtaaacata tattattaat aactgggata gcacaaccca aaccaatgta
      721 tgattttata tcggaatatt gcaaaaatat tacccactta aattttgctg accatcatat
      781 atatactaaa gaggactatg acgtaatact gcaaaactat aaaacgtatc gcaccacaat
      841 agttacaaca gaaaaagatg ctccaaaaat tcttgaaatt acacgtaagc aattacctgt
      901 ttttgtactt tcaattgata ttgaattttt gaataatacc gagcccgaat ttcagaataa
      961 aatagtagat tttgtttcac aatgaattct ttagctcata cttttttgtc gggtatagac
     1021 aaacaagtaa taattggaaa ttttataggt gattttgtaa aagggcaatc atattcacaa
     1081 tttcccgtag gtattcaaaa aggcatttta ctacacagaa gtattgatag ttttgccgat
     1141 aattctccta ttgtaaaaaa aagcaaagaa ttttttaaac ctttatatgg acgttatgcc
     1201 ggaataatag tagatatagt ttatgatcat tttctatcaa cacattggca cctatattct
     1261 gccatgcgtc gagaatattt tatacaatat gtatattcat gtttatgcca atattcctgc
     1321 attttaccta aacgagcaca acaattaata ccatcaatag catacaacaa ttggatgaga
     1381 tattatgcat catattatgg attggtacta ggctgttttt ttaaaacaag ttaaaaatag
     1441 attttttact tttaact
//
LOCUS       DAOTOW010000649         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69634_length_1456_cov_2.920771, whole genome shotgun sequence.
ACCESSION   DAOTOW010000649 DAOTOW010000000
VERSION     DAOTOW010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69634_length_1456_cov_2.920771"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="P5243_10115"
     CDS             <1..698
                     /locus_tag="P5243_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRS19849.1"
                     /translation="QVASTNGILFQDLDNDLLTTNTPKGVTVCSYSIEHKSNVCGTVH
                     QKSFFAAFTLSVGNDSIDITSQLFGNYNIKNMLAAACIGNYFAVPLLDIKNALESYSP
                     SNNRSQLQKTQNNTLILDMYNANPTSMRAALEQFVTIANLNSAVILGEMYELGEISYD
                     EHSKIIEFVRNSSISKILLVGNWPKVEDSKIQYFIKSTDLLEYLSKESLHNYTILIKG
                     SRGVKLETVLPSL"
     gene            complement(693..>1456)
                     /locus_tag="P5243_10120"
     CDS             complement(693..>1456)
                     /locus_tag="P5243_10120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS19850.1"
                     /translation="ILLNSDVEVNPHWITPLYTYMQNNPQTAVCMPKIKSYNNKNFFE
                     YAGAAGGYIDKYGFPFCKGRIFNSIEEDLGQYNNQYEIFWASGAALFVRTSIYKELGG
                     LDADFFAHMEEIDFCWRAKNSGYTCMYIPQSQVFHVGGGTLPQSNPFKTYLNFRNNLF
                     LLYKNLPQSKLKTTIWIRKIFDGIAWLQFLLQGKWGDCKAILKAHRDYYKALPQLHTK
                     RTTCNHTKSFESYIHMYQSSIVWQYFIRNKKSLKL"
BASE COUNT          494 a          216 c          224 g          522 t
ORIGIN      
        1 cgcaagttgc ttcaactaat ggtatattat ttcaagattt ggataatgac cttttaacaa
       61 ccaatacacc taaaggtgtt acagtttgtt catattcaat agaacataag agtaatgtat
      121 gtggtacggt acatcaaaaa tcattttttg ctgcatttac gcttagtgtt ggcaatgatt
      181 ctattgatat aacatctcaa ctatttggaa attataatat aaaaaatatg ttagcggcag
      241 catgcatagg aaactatttt gctgttccat tactcgatat taaaaatgca ttagaatcat
      301 atagtccttc aaataataga tcgcaactac aaaaaacgca aaacaataca ttaattcttg
      361 atatgtataa tgcaaatcca acaagtatgc gtgctgcatt agagcaattt gttactattg
      421 ccaatcttaa ttctgctgtg attttaggtg aaatgtatga acttggtgaa atttcatatg
      481 atgaacactc aaaaataatt gaatttgttc gaaattcatc tatatcaaaa atattattag
      541 ttggtaattg gcctaaagtt gaagattcta aaattcaata ttttataaag tctactgatt
      601 tattagaata tctatcaaaa gaatcactac ataattatac aattcttatt aaaggttctc
      661 gtggggtaaa attagaaaca gtattaccaa gtttataatt tcaaactctt tttattgcgt
      721 ataaaatatt gccatacaat agatgattga tacatatgta tataactttc aaaactcttt
      781 gtatggttgc atgtagttct tttggtgtgt aattgtggta atgctttata ataatctctg
      841 tgtgccttta aaattgcttt acaatcgccc catttacctt gtaaaagaaa ttgtagccat
      901 gcaatgccgt caaatatctt acgtatccaa atagtagttt ttagttttga ttgaggaaga
      961 tttttataga ggagaaatag attgtttctg aagtttaaat atgttttaaa cggatttgat
     1021 tgaggtaatg tacctccgcc aacatgaaat acttgtgatt ggggtatata catgcatgta
     1081 tatcctgaat tttttgctct ccagcaaaaa tcaatttctt ccatgtgggc aaaaaaatcg
     1141 gcatctaatc cacctaattc tttatatata gatgttctta caaacagagc tgctcccgaa
     1201 gcccaaaata tttcatattg attgttgtat tgccccaagt cttcttctat tgaattgaaa
     1261 atcctgcctt tacaaaatgg aaatccatat ttatcaatgt agccacctgc agcaccggca
     1321 tattcaaaaa agtttttatt attatatgat ttaattttag gcatacatac agcagtttgt
     1381 ggattgtttt gcatatatgt atacaatgga gttatccaat gagggttaac ttcaacatct
     1441 gaattgagta aaatgt
//
LOCUS       DAOTOW010000650         1454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69748_length_1454_cov_3.684775, whole genome shotgun sequence.
ACCESSION   DAOTOW010000650 DAOTOW010000000
VERSION     DAOTOW010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69748_length_1454_cov_3.684775"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..453)
                     /gene="trxA"
                     /locus_tag="P5243_10125"
     CDS             complement(157..453)
                     /gene="trxA"
                     /locus_tag="P5243_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRS19851.1"
                     /translation="MNAFYETIQGTTPVLVDFYADWCQPCKMQAPILQDFAKRMNGKV
                     RVLKINVDKNPQVAAKYGIRSIPTLVLFKQGNAVWRGAGVHQTKQLEQVVGGYV"
     gene            complement(805..1044)
                     /locus_tag="P5243_10130"
     CDS             complement(805..1044)
                     /locus_tag="P5243_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HRS19852.1"
                     /translation="MVHDTFAQKSTMTFLLKYKIEIITAILGGIGGYAYWFYIGCSTG
                     ACPITANWHTSVIYGIIMGFLVGQTITDYTKKKEV"
     gene            complement(1067..>1454)
                     /locus_tag="P5243_10135"
     CDS             complement(1067..>1454)
                     /locus_tag="P5243_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19853.1"
                     /translation="CMTNVTAEIYARSVEQCTIIKIPIQNVDMWFTRYSSWKQFVFMQ
                     YRMRFNELLQTIDSLAFLKMDERLIRFLKNIYTNTGKTVYEGSHADIALALSSSREVI
                     SRLLKTLEHNKKVVLSRNKIDFSQLV"
BASE COUNT          443 a          297 c          225 g          489 t
ORIGIN      
        1 ggcatgataa tatctatgaa tttcattcaa gatttatttg taattttggg tggaagtatt
       61 aatttgaatt tgcatgcccc tcaactttgt tcgacgaata cacatataac tctgtactag
      121 tcgaacgaag gaaatttgaa aaaatacata agcagcctat acataacccc caactacttg
      181 ttcgagttgc tttgtttgat gcacgcccgc tccacgccat acggcattgc cttgtttaaa
      241 caatactaat gtaggtatac tccgtatacc atatttggca gctacctgcg ggtttttgtc
      301 aacatttatt tttaacacac gcactttccc attcatgcgt ttagcaaagt cttgcaatat
      361 gggagcttgc attttgcatg gctgacacca atcggcataa aaatctacta atacgggcgt
      421 agttccttgt attgtttcgt aaaatgcatt catagttttt gaacagtttt atcttaatgt
      481 caccccttcg gggttttttg tgataataaa atatattgta ctctataata atatcatcct
      541 ttcgggattt acaattaacc ttcaagactt ttcagactac catactgtca ccccttcggg
      601 gtttttgatg gttataatga ttcttatgtt ctataataat atcatccctt cgggatttaa
      661 caattgactt ttcagacctt ttaatatttt tgccacgaag acactaaggc acaaagattt
      721 aaactaatgt atgaattcta ccatactctc atctatcggg ataaaaaaat tgacttttta
      781 gaccttttag actcttcaaa cccttcagac ctcttttttc ttcgtataat cagtaatcgt
      841 ttgtcctacc aagaagccca taataattcc gtaaattacg ctggtatgcc aattcgccgt
      901 tataggacaa gcacccgtag aacaaccaat ataaaaccaa tatgcataac cacctatacc
      961 accaagtatg gcggtaataa tttcaatttt gtatttcaat aaaaatgtca tagtactttt
     1021 ttgtgcaaat gtatcatgta ccaataccgc aagtctgtaa cctgtgtcac acgagctgag
     1081 aaaaatctat tttgttgcgc gataacacaa cctttttatt gtgttccaag gttttgagca
     1141 atcgcgaaat tacttcgcgc gatgaactta atgcaagtgc aatatcagca tgactacctt
     1201 cgtacaccgt tttgcccgtg ttggtatata tatttttcaa aaagcgaatc aagcgttcat
     1261 ccattttcaa aaaagccaaa ctatcaatag tttgcagcaa ttcattgaaa cgcatacgat
     1321 attgcataaa tacaaattgt ttccacgaac tataacgtgt aaaccacata tctacgttct
     1381 gaattggaat ttttataatt gtacattgct caaccgaacg ggcatatatt tcggctgtta
     1441 cattggtcat gcaa
//
LOCUS       DAOTOW010000651         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_69824_length_1453_cov_3.438484, whole genome shotgun sequence.
ACCESSION   DAOTOW010000651 DAOTOW010000000
VERSION     DAOTOW010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69824_length_1453_cov_3.438484"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="P5243_10140"
     CDS             <1..896
                     /locus_tag="P5243_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19854.1"
                     /translation="LLEPTMTIYYYDQNPMPLYKPLLHVYNSNNYIGFTVFFTAIILC
                     LILLSYINSTFRMLEKRSSLYLLLFIVFSSFIPQFQQFNPMPFACIFVLLGIYSIYKL
                     YKNEYELRAVFEAGFCFAIASLLYIQTIFISIFIFAGIIQLVPFYWRQWIAGLFGLVT
                     PYIFVVSWAFINNSLPKLLETIDANIVVWHSWGEISLPFYISIISIISIFIISLLFSF
                     SGALKKVALQKYYLLQFFLFLFVIIIFAIIPGAGIEIFYFSVIPLSFYITNYLLNIRN
                     TLIPEIITFILIGLRIFLFIA"
     gene            complement(1099..1347)
                     /locus_tag="P5243_10145"
     CDS             complement(1099..1347)
                     /locus_tag="P5243_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRS19855.1"
                     /translation="MFYTYVLYSQEYNKTYVGYTSNLDGRLQSHNHPQNKGWTRKFQP
                     WEIIYFEEFHEKKEAMIREKVLKTGKGREFIQNILQNR"
BASE COUNT          477 a          211 c          194 g          571 t
ORIGIN      
        1 atctattgga acctacaatg accatttatt attatgatca aaaccctatg ccattatata
       61 agccattgtt gcatgtatat aactcaaata attacatagg gtttactgta ttttttacag
      121 ctataatttt gtgtttaata ttactgtcat acataaattc aacttttcga atgttggaaa
      181 aacgaagttc attatattta ttattattta tagttttttc atcatttatc ccacaatttc
      241 aacaatttaa tccaatgcct tttgcatgca tttttgtatt attaggtatt tattcaattt
      301 ataaattata caaaaatgaa tatgaattga gagctgtatt tgaagccggc ttttgttttg
      361 caatagcttc attactatat atacaaacaa tttttatttc tatctttata tttgcaggta
      421 ttattcaatt agttccattc tattggagac aatggattgc cggtttattt ggtttagtta
      481 cgccttacat ctttgtagta tcatgggcat ttattaataa tagtttgcca aaacttttag
      541 aaactataga tgcaaatatt gttgtatggc attcttgggg cgaaatttct ttaccttttt
      601 atattagtat aatcagtatt ataagtattt ttattatatc attattattt tcattttcag
      661 gggcgttaaa aaaagttgct ttacaaaaat actatttact tcaatttttt ctatttctat
      721 ttgtaattat aatatttgcc ataattcccg gtgcaggtat tgaaatattt tattttagcg
      781 ttatacctct cagtttttat attacaaatt acctactcaa tatacgaaac acattaattc
      841 ctgaaattat aacctttata ttaataggat tacgcatatt cctatttata gcataatctt
      901 ccatctaaat tcgaagtata tcccacatag gttttgttgt attcttgaga atacagtaca
      961 taggtgtaaa acatgataaa aaagtatgtg ttgcaaataa aaaaggctat ctataaatat
     1021 agatagcctc ggtagtttcc gcttaagcga gactagactc gaaccgacgt ccgcccgggc
     1081 ggatatgagc aactcgaact atctgttttg taaaatgttt tgaataaatt ctctaccttt
     1141 gcctgttttt aatacttttt ctctaatcat tgcttctttt ttttcatgaa attcttcaaa
     1201 ataaatgatt tcccaaggtt gaaactttct tgtccaccct ttgttttgag gatgattatg
     1261 tgattgtaat cttccatcta aattcgaagt atatcccaca taggttttgt tgtattcttg
     1321 agaatacagt acataggtgt aaaacatgat aaaaaagtat gtgttgcaaa taaaaaaggc
     1381 tatctataaa tatagatata cgggcactaa aacaaaatgt acaatataat aaacaataaa
     1441 aaaaggtcga aaa
//
LOCUS       DAOTOW010000652         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70015_length_1451_cov_2.435530, whole genome shotgun sequence.
ACCESSION   DAOTOW010000652 DAOTOW010000000
VERSION     DAOTOW010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70015_length_1451_cov_2.435530"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1451
                     /locus_tag="P5243_10150"
     CDS             <1..>1451
                     /locus_tag="P5243_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HRS19856.1"
                     /translation="ITANTQGTHDGFFYSFWTEIANNGASMTLGPGGNYSTVWNNTQN
                     FTAGKGWKVGTANRVVCFEGTYNGGSNGFLALYGWTKDPLIEYYVCEKHGQWEPPGNT
                     SDIIYKGTYTCDGGTYKCYTAKRVNKPSIIGTATFDQYWSVRVEQRSSGTITFANHIA
                     AWKSYGMNMGTTWDYQIMESEGYNSSGSSNITVSECQASPITVAITAPTATTSFSAPA
                     TVNITATASTTTGTISKVEFYNGTTKLGEDATSPYTYSWTNVAAGTYSITAVATNSAG
                     TKATSTAVSIKVNVPRGPYGGTAHAIPGKIEFENYDVGGNGIAYLDNTAGSSVASPPN
                     FRTDEDVDIETCTDAGGGYNIGYATAGEWLEYTVNVATTGTYNVTFRVACSGTGRTMS
                     LAANNVTVVNNLSIPNTNGWQTWQDVTVQNIQLTAGTQIIRLTIGATDYINLNYMTFT
                     ASGTPAPTVTTPVTYCQGATATALTATGTALKW"
BASE COUNT          511 a          242 c          314 g          384 t
ORIGIN      
        1 tattacagca aacacacaag gtactcacga cggctttttt tattcatttt ggaccgaaat
       61 tgcaaataat ggagcttcta tgacattggg ccctggtgga aattacagta cagtatggaa
      121 taatacacaa aactttacgg caggtaaagg ttggaaagtt ggtacagcaa acagagttgt
      181 atgttttgaa ggaacatata atggagggag caatggattc cttgcattat atggttggac
      241 aaaagatcct cttatagaat attatgtgtg tgaaaaacat ggacagtggg aaccaccggg
      301 taatacaagt gatattattt ataaaggtac atatacttgt gatggtggaa catataaatg
      361 ttatactgct aaacgagtaa ataaaccttc tattattggt actgcaactt ttgaccagta
      421 ttggagtgtt cgtgtagaac aacgttcttc aggaactata acttttgcaa atcacatagc
      481 tgcatggaaa agttatggta tgaatatggg aacaacatgg gattatcaaa ttatggaatc
      541 tgaaggatat aatagttccg gaagttctaa tataactgta agtgaatgtc aagcatcacc
      601 tattaccgta gctattacag ctcctacagc aacaacttcg ttttcagcac cggctacagt
      661 aaatataaca gctacagctt caactacaac cgggacaata tcaaaagttg agttttataa
      721 tggtactacc aagttaggtg aagatgctac atcgccatat acatattcat ggacaaatgt
      781 agcagcagga acctattcaa ttactgcagt tgcaaccaat agtgccggaa ccaaagcaac
      841 atcaacagcg gtaagtatta aggtaaatgt accaagagga ccgtatggag gtacagctca
      901 tgcaataccg ggaaaaatag agtttgagaa ttacgatgtt ggaggaaatg gaattgctta
      961 tttagataat actgcggggt ctagtgtagc aagtcctccg aattttagaa cagacgaaga
     1021 tgtagatata gaaacatgta ccgatgccgg aggaggatat aatataggat atgcgacagc
     1081 cggcgaatgg ttagaatata cagtaaatgt tgcaacaaca ggtacatata atgttacatt
     1141 tagggtagca tgcagtggca caggacgaac aatgtcgttg gctgccaata atgtaacagt
     1201 agttaataat ttatcaatac ccaataccaa tggttggcaa acatggcaag atgtaacagt
     1261 acaaaatata caacttacgg cgggtacaca aattattaga ttaacaatag gagcaacaga
     1321 ttatataaat ttgaattata tgacgtttac cgcgtctgga actcctgcgc caacagtaac
     1381 aacgcctgta acgtattgtc aaggagctac agcaactgcg ttaacagcaa ccggaacggc
     1441 attgaaatgg t
//
LOCUS       DAOTOW010000653         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70084_length_1450_cov_2.919713, whole genome shotgun sequence.
ACCESSION   DAOTOW010000653 DAOTOW010000000
VERSION     DAOTOW010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70084_length_1450_cov_2.919713"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="P5243_10155"
     CDS             <1..971
                     /locus_tag="P5243_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS19857.1"
                     /translation="KTMKLSGITFLPTYNCNASCSHCFFDTKACKQYMEPDIVHKVFE
                     NYKITKFMTWVHVSGGEILLNQRACFDLIETIQLYFKKNIGISTNAFWATSSSVAYDV
                     VQTLVSLGVNGIAVSADYFHSPYVPLSHIKRAVQAIVDSGITTHCYIMGAQCKSDVLN
                     SNDINSVSQQIAAYVKGNTNMPLAPTNIRSIGYGSKINSPKKQIVPNGKCTDLSECLG
                     KRGPLNPKMVWIDCYGNVMICYGIIIGNIYKTSFAEIIANYSPKQSIIIDILTEKGPI
                     GLYELAQQMNVAVVKEFYDECDVCYSSRKALQPFFSELGPAECYPY"
     gene            complement(985..>1450)
                     /locus_tag="P5243_10160"
     CDS             complement(985..>1450)
                     /locus_tag="P5243_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19858.1"
                     /translation="GTTKVGNDLKSSTAYNCVETDYHIIHKQGFTDTQILQRITHKPF
                     LSITQAYTRCSLIYVGNKCAITSDNNIHTRLIDTGYSSLLCNPHGILLPPYKHGFIGG
                     CFGMHSNTLFCIGNLDYLNDGKRIRAFLDTHNIPLIELYNGPLYDGGGIFFI"
BASE COUNT          500 a          202 c          270 g          478 t
ORIGIN      
        1 ataaaacaat gaaactttcc ggaattactt ttttacctac atacaattgc aatgcatctt
       61 gttctcattg tttttttgat acaaaagctt gcaaacaata tatggaacca gatattgtac
      121 acaaagtttt tgaaaattac aaaatcacaa aatttatgac atgggttcat gtaagtggcg
      181 gcgaaatatt attgaaccaa agagcatgtt ttgatttaat tgaaacaatt caactgtatt
      241 tcaagaaaaa tataggtatt tcaacaaatg ctttttgggc tacaagttca agtgttgctt
      301 atgatgtagt gcaaacatta gtgtcactgg gggtaaatgg tattgctgtg agtgcggatt
      361 attttcattc gccgtatgtg cctttatcgc atataaaaag ggcagtgcaa gctatagttg
      421 acagtggtat tacaactcat tgttatataa tgggtgcaca gtgtaaatca gatgtattga
      481 atagtaacga tataaatagt gtaagtcaac aaatagcagc ttatgtaaaa ggaaatacaa
      541 atatgccatt ggctcctact aatatcagaa gtatcggcta tggcagtaaa ataaattcac
      601 ctaaaaaaca aattgtacct aatggaaaat gtactgattt atcagaatgt ttaggaaaac
      661 gaggaccttt gaatcccaaa atggtgtgga tagactgtta tggaaatgta atgatatgtt
      721 acggaattat aataggaaat atatataaaa cgtcatttgc tgaaattatt gcaaattatt
      781 cacctaaaca aagtattatt attgatattc taaccgaaaa aggtccaatt gggttatatg
      841 aacttgctca acaaatgaat gtggcagttg taaaagaatt ttatgatgaa tgcgatgtgt
      901 gttatagcag tcgtaaagca ttgcaaccgt ttttttcaga attaggaccg gcagaatgtt
      961 atccgtatta aatgatactc ataattatat aaaaaatata ccaccgccat catacagagg
     1021 tccgttatat aattctatta gaggaatgtt gtgtgtgtct aaaaatgctc gtatacgttt
     1081 tccatcattt aaataatcta aattaccaat gcaaaacaaa gtattggaat gcataccaaa
     1141 acacccccct ataaatccat gtttataagg aggtagtaaa attccatgtg gattgcacag
     1201 gagagaagag tatccagtat caattaatcg tgtatgtatg ttgttgtcag atgttatagc
     1261 acatttgttg ccaacatata ttagactaca gcgtgtatat gcttgtgtaa ttgaaagaaa
     1321 tggtttgtgc gtaatacgtt gtaatatttg tgtatcggta aatccttgtt tgtggattat
     1381 atgataatct gtttctacgc aattgtatgc agttgatgat tttaagtcat tccctacttt
     1441 ggtggtgcct
//
LOCUS       DAOTOW010000654         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70092_length_1450_cov_2.618638, whole genome shotgun sequence.
ACCESSION   DAOTOW010000654 DAOTOW010000000
VERSION     DAOTOW010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70092_length_1450_cov_2.618638"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="P5243_10165"
     CDS             complement(<1..524)
                     /locus_tag="P5243_10165"
                     /inference="COORDINATES: protein motif:HMM:NF039908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HRS19859.1"
                     /translation="MILQFITFRFLDFIDIFLVALLMYQLYKLIRGTVGMSIFAGIVA
                     LYAIWLIVKATKMELLTTILGQFVGVGVLALIILFQQEIRRFLIIVGTRYFFKKRRFG
                     FEKLFNLNLNPMSKEDIQMLVHAVFTMAEQRTGALIVISPTIEMDSYIQTGEVLDAKI
                     SSSLLQSIFYKNSP"
     gene            complement(568..>1450)
                     /locus_tag="P5243_10170"
     CDS             complement(568..>1450)
                     /locus_tag="P5243_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS19860.1"
                     /translation="LMLATIFAVFISNKITYPLTILRDKMKLVKVGSKNEKIQWSAPD
                     EIGELIENYNSMIDQLDLSVKKLAESEREGAWREMAKQIAHEIKNPLTPIKLNLQLLN
                     KAWEQHDEQFEKRLKTISKSIIEQIDALAETANSFSNFAKLTEGNPETIVLNDLLTGC
                     ITLFENEQHITFISYIPDEVIEIYADRDKMLRLFNNIIKNAIQAIPLNTQGTITITVE
                     HTHSKVLVSIADTGTGITPEVADKLFEPYFTTKSTGSGFGLAISKKIIEMSGGTIWFT
                     TKVGEGTVFFIELPTCT"
BASE COUNT          467 a          254 c          200 g          529 t
ORIGIN      
        1 aaaggactat ttttataaaa aattgactgc aataatgacg aacttatttt tgcatctagc
       61 acttcgcctg tttgaatata tgaatccatt tcaatagttg gagatattac aataagtgca
      121 cctgtgcgtt gttctgccat agtaaaaact gcatgtacca acatttgaat atcctcttta
      181 ctcataggat tgaggtttaa attaaataat ttttcaaacc caaatcttct ttttttaaaa
      241 aaatatcgtg ttcctacgat tattaaaaag cggcgaattt cttgctgaaa caaaataata
      301 agagccaaca ccccaactcc tacaaattgc cctaaaatag tagtaagtaa ctccattttt
      361 gtagctttta ctatgagcca tatagcatac aatgctacaa ttccggcaaa tatactcata
      421 cctacggtgc ctcgtatgag cttatataat tgatacataa ggagtgctac caaaaatata
      481 tcaataaaat caagaaatcg gaatgttata aactgtagaa tcatacacaa attttacata
      541 tcaaaaatag taaatagttt taatttttta tgtacaggta ggtaattcta taaaaaacac
      601 tgttccttct cctactttag ttgtaaacca aattgtaccc ccggacattt ctattatttt
      661 ttttgaaatt gccaaaccaa aaccacttcc ggtagattta gtagtaaagt atggttcaaa
      721 taatttatcg gcaacttcag gagttattcc agtacctgta tcagcaatac ttacaagcac
      781 ttttgaatga gtatgctcta ctgtaattgt tatagtacct tgtgtattaa gtggaatagc
      841 ttgtattgca ttttttatga tattattaaa caatcgcaac attttatctc tatcggcata
      901 tatttcaata acttcatctg gtatatatga aataaatgtt atatgttgtt cattttcaaa
      961 caacgtaata catcctgtca ataaatcatt taacactatt gtttcgggat ttccttcggt
     1021 taattttgca aaattcgaaa atgaattagc agtttccgcc aatgcatcta tttgttcaat
     1081 aatagatttt gatattgttt ttaagcgttt ttcaaactgt tcatcatgtt gctcccatgc
     1141 tttgttgagc aattgtaaat ttaacttgat tggagtaagt ggattcttaa tttcgtgagc
     1201 tatttgtttt gccatttcac gccacgctcc ttctcgttca gactctgcca atttttttac
     1261 acttaagtct aattggtcaa tcatactgtt ataattttca ataagctcac ctatttcatc
     1321 aggggcactc cactggattt tttcattttt tgacccaact tttaccaact tcattttgtc
     1381 gcgcaatata gtaagcggat aagttatttt attagatata aatactgcaa atatggttgc
     1441 caacattaac
//
LOCUS       DAOTOW010000655         1448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70259_length_1448_cov_2.996411, whole genome shotgun sequence.
ACCESSION   DAOTOW010000655 DAOTOW010000000
VERSION     DAOTOW010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70259_length_1448_cov_2.996411"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1448
                     /locus_tag="P5243_10175"
     CDS             68..>1448
                     /locus_tag="P5243_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS19861.1"
                     /translation="MRIFIYCFLLIFAIPHTVGAQNTSKLFEEGKQAYFKQDYQKSIA
                     IFEELLEQYEKTPLIYLYLAHSYRLQLRYNEALNMYSKLIHISPNKYPECYFYLAELY
                     EILGSQSQAKHYYSRFIQFSKQDEQLFAFATKKISLYEKQHTLPYIDSINLYEDIGSP
                     FFQNLSVQKISNSIVYNGLIIRNDTITNATFFSTDSALSKQLNSYFTQLNFTYTDLTP
                     TNIQNQFWINRRNAPIYSNLPQLYSFFITKDSISEFRQITIPQTQNYLVIHPHFCIFQ
                     DSIRIFFSSNLPNGYGGMDIWYCVQDSLGNLSNPINAGKSINSSYDEICPFYNSKNAT
                     LYVSSNRPESIGGFDIFGIQINEAQTTIFQLPQPYNSSFNDLYFRIFDTIQFLTSNRK
                     PKQIQSSSYYVNSICYSILPKPAITNTSPPKKTIKHIEPISIFFDFDKPTPHDTMNYT
                     HQYNSFYNSR"
BASE COUNT          539 a          234 c          153 g          522 t
ORIGIN      
        1 ttgttttttc aaaaaaatat tgattaaaat atatattttt gaaagaatat tttcaaggta
       61 ttatagtatg cgaatattta tctattgttt cttattaatt tttgcaatac ctcatacagt
      121 tggtgctcaa aatacttcaa aattatttga agaaggcaaa caagcatact ttaaacaaga
      181 ttatcaaaag tcaatagcaa tatttgaaga attacttgaa caatatgaaa aaacccctct
      241 tatatattta tatttagctc atagttaccg attgcaattg cggtataatg aagctttaaa
      301 tatgtattct aaacttatac atattagtcc aaataaatac cctgaatgtt atttttattt
      361 agctgaatta tacgagatac tcggctcgca atcacaagca aaacactatt attccagatt
      421 tatacaattt tcaaaacaag atgagcaatt atttgctttt gctacaaaaa aaatttcact
      481 atatgagaaa caacatactc taccctatat agatagtatt aatttatatg aagacattgg
      541 aagcccattt tttcaaaatc taagtgttca aaaaatttcg aactctattg tttataatgg
      601 attaatcatt agaaatgata ctattactaa tgcaacattt ttttcaactg attctgcctt
      661 atcaaaacaa ttaaactctt actttacaca attaaatttt acatataccg acctaacacc
      721 aacaaatatt caaaatcaat tttggataaa cagacgtaat gctccaattt atagcaattt
      781 acctcaatta tattcttttt ttattactaa agattcaata tcagagttta gacaaataac
      841 tatacctcag actcaaaact atttagtaat ccatcctcat ttttgcattt ttcaagattc
      901 aatacgaata tttttttcat caaatttacc aaatgggtat ggtggaatgg atatttggta
      961 ttgcgtgcaa gactctttgg gtaatttatc taatcctata aatgcgggta aatccataaa
     1021 tagcagttat gatgaaattt gtccatttta taactcgaaa aatgctactt tatacgttag
     1081 ttctaacaga ccggaatcta taggaggatt tgatattttt ggaattcaga ttaatgaagc
     1141 acaaactact atttttcaat tgccgcaacc atataattca tcttttaatg atttatactt
     1201 tagaattttt gatactattc aatttcttac atcaaataga aaacctaaac aaatacaatc
     1261 atcatcatat tatgtaaata gtatatgtta ttctatactc cccaaacctg ctattacaaa
     1321 tacatcgccc cccaaaaaaa caataaaaca tattgagccg atttctatat tttttgattt
     1381 tgataaacct acacctcatg atacaatgaa ttatacccat caatataatt cattctataa
     1441 ttctcgca
//
LOCUS       DAOTOW010000656         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70320_length_1447_cov_3.677443, whole genome shotgun sequence.
ACCESSION   DAOTOW010000656 DAOTOW010000000
VERSION     DAOTOW010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70320_length_1447_cov_3.677443"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="P5243_10180"
     CDS             complement(<1..1439)
                     /locus_tag="P5243_10180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRS19862.1"
                     /translation="MEGIDKTWIPADTKNRIASYSALPPGTYIFRVRAGDGHGTWSAE
                     RKLKVTVIPPWWKTWWLRIGLLLCIITIVWWIIYYRFSLIKQQSQLLDNKVKERTRDL
                     LVANKKLYATNEQLKQNQIVIEMRNADLNQALQTKDELIKILGHDFKNPLTGILGLAD
                     LLKTEKIQTNQKKVTHFAELIYKSTTTLLNQMATVLEWAQSSNKNLEANPIEINIETL
                     VDDAISLIKENAVHKKITISKQTDFEYTVLVDPRMIGTVLRNILTNAIKYSHKGSSIL
                     IIIQEIDSTIDINVIDTGIGMNQETLSCIHSAKIIQSSPGTADEKGTGFGLHICKTFI
                     EKNHGKLSIVSEENKGTIVTVSLPKGLQKVSSSPVNSTEASFEPIEHEKKHESTILII
                     DDSIEIREIIQGIFDSNYTVIQSDNGKEGLYIAQHLLPDIIISDIMLPGIDGYEICKT
                     IKTNDVTKHIPVLLISSQIGLDTETKSFEA"
BASE COUNT          417 a          255 c          212 g          563 t
ORIGIN      
        1 gcttcaaaac ttttagtttc ggtatctaat cctatttgcg atgaaataag caaaacagga
       61 atatgtttgg ttacatcatt cgttttaatg gttttacaaa tttcataacc atcaataccc
      121 ggcagcataa tatcagaaat tataatatcg ggcaataaat gctgagcaat atacaaccct
      181 tctttaccat tatctgactg aataacagta tagttagaat caaaaatacc ctgtataatt
      241 tctcttattt ctattgaatc gtcaataatt aatatggtgc tctcatgttt cttttcatgt
      301 tctataggtt caaaagatgc ttcggtggaa ttgacaggtg aagaacttac tttttgtaaa
      361 cctttgggta atgaaactgt tactatggtt cctttatttt cttcacttac aattgaaagc
      421 tttccatgat ttttttcgat aaatgtttta caaatgtgta atccaaaacc ggttcctttt
      481 tcatcggcag ttcccggact actttgaatt atttttgcag agtgtataca cgacaacgtt
      541 tcttgattca tgcctatacc ggtatcaata acattaatat caatggtaga atcaatttct
      601 tgaataatta ttagtataga actgccttta tgcgaatatt taattgcatt cgttaaaata
      661 ttcctcaaaa ctgttcctat cattcgtggg tcaaccaata cggtatattc aaaatctgtt
      721 tgcttagata tggtaatttt tttgtgtaca gcattttctt ttattaaact tattgcatca
      781 tctaccaagg tttcaatatt tatttcaata ggatttgctt cgagattttt atttgaactt
      841 tgtgcccatt ccaacacggt agccatttga ttgagcagcg ttgttgtact tttatatatt
      901 aattcggcaa aatgtgtaac ttttttctga ttggtttgaa ttttttccgt tttaagcaaa
      961 tcggccaatc ccaaaattcc tgttaatgga tttttaaaat catgtcccaa tattttaata
     1021 agttcatctt ttgtttgcaa tgcctgattt aaatcggcat tacgcatttc tataactatt
     1081 tgattttgtt tgagttgttc gtttgttgca tataattttt tatttgctac caataaatca
     1141 cgagtacgtt cttttacttt attatctaat aattgacttt gttgtttaat caacgaaaat
     1201 ctatagtata ttatccacca aactatggta attatacata acaatagccc tattcgcaac
     1261 caccacgttt tccaccatgg cggtattaca gttactttca gttttcgttc tgcactccat
     1321 gtaccatgac catcaccggc tcgtactctg aatatatatg tgcccggagg caaggcagaa
     1381 tatgaagcga ttctattttt ggtatcggcc ggtatccatg ttttatctat accttccata
     1441 atgtatt
//
LOCUS       DAOTOW010000657         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70620_length_1443_cov_3.219020, whole genome shotgun sequence.
ACCESSION   DAOTOW010000657 DAOTOW010000000
VERSION     DAOTOW010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70620_length_1443_cov_3.219020"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1443)
                     /locus_tag="P5243_10185"
     CDS             complement(<1..>1443)
                     /locus_tag="P5243_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS19863.1"
                     /translation="TAEGNFKDSVYIKVISSEPPVVKDIEVVMEKGSSQVEVPLQSFV
                     KDDNTAIEYLTVSVVSSTNFTASVQNGAIVIQPVNPSIPITEVVQVKVTDIDNLSTTV
                     EIPVTISGTPNQAPTFEPEVVVTLNSGTSFAPIVLQDLVQDDYTAASNLTYEIVTPSQ
                     NFITYVSNGVLDVTRIDANWVGKDSVEVKVTDGAGLSTTKFIVLEVSQALNQAPVLNS
                     IPAQTYNVTTARYPSLDLKKYVKDDYTPASAIEWTFTPNSKLLITIVNGVASILSADK
                     NWVGTTQVTFTAFDQDGLSASTVVSFTQPATGSSTWMALPKISFSASQTIVGPGAPVS
                     FTSSMSGAESWIWEFEGLNLTPQQRIVPNPVVSYSKGGKYKVTLKAMNQYGADSLVKV
                     DYITVIGINKVNPIVCYGSSVTLEATVPASAGYSYVWSTGENSQSITVQPTANTTYSL
                     TVYKGFFQYEDAVSLQVPVKVSLPADRAICQ"
BASE COUNT          479 a          264 c          238 g          462 t
ORIGIN      
        1 ttgacaaata gctctatctg caggtaatga tacttttacc ggaacttgta aagaaacagc
       61 atcttcatat tggaagaatc ctttatatac agtaagacta tatgtagtat ttgcagtagg
      121 ttgtacagta atagattgtg aattttctcc ggtactccac acatatgaat accctgccga
      181 tgcaggtaca gtagcttcaa gtgttactga acttccataa caaactatag gattaacttt
      241 atttatacca attacagtta tataatctac cttaaccaaa gaatcagcac catattgatt
      301 catagctttg agtgtaactt tgtatttacc acctttgcta tatgatacca caggatttgg
      361 cacaattcgt tgctgtggag ttaaattcaa accttcgaac tcccaaatcc atgattcggc
      421 acccgacata gatgaagtaa atgaaactgg agcaccagga ccaacaatag tttgcgatgc
      481 cgagaatgat attttaggta atgccatcca tgtagaacta cctgttgccg gttgagtaaa
      541 cgaaacaaca gtagatgcac tcaatccatc ttggtcaaat gcagtaaagg taacttgagt
      601 agttccaacc cagtttttat cggcagaaag tatagaagcc actccattta ctattgtaat
      661 taacaatttt gaatttggag taaatgtcca ttcaatagca gaagcaggag tataatcatc
      721 tttcacatat tttttaagat ctaaacttgg atatcgagct gttgtaacat tatacgtttg
      781 agcaggtatt gaatttagca ccggagcttg attaagagct tgagatacct ctaaaacaat
      841 aaatttagtt gttgaaagcc ctgcaccatc tgttactttt acctcaacag aatctttacc
      901 aacccaattt gcgtcaatac gagtaacatc taatacacca ttagacacat aagtaataaa
      961 attttgtgaa ggtgttacaa tttcatatgt taaattactt gctgcagtat aatcatcttg
     1021 caccaaatct tgtaaaacta ttggagcaaa tgaagttcct gaatttaacg ttacaaccac
     1081 ttcaggttca aacgttggtg cttgatttgg agttcctgaa attgtaacag ggatttctac
     1141 agttgttgac aaattatcaa tatcagtaac ttttacttga actacttcag ttatagggat
     1201 tgatggattt acaggttgta ttactatagc accattttga acagaagctg tgaaattagt
     1261 agaacttact acagatactg ttaaatattc aatagcagta ttatcatctt tcacaaaact
     1321 ttgtaaaggc acttctactt gtgatgaacc tttttccata accacctcaa tatctttaac
     1381 aacaggaggt tctgacgaaa ttactttaat ataaacagaa tctttaaaat taccttcggc
     1441 agt
//
LOCUS       DAOTOW010000658         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70625_length_1443_cov_2.861671, whole genome shotgun sequence.
ACCESSION   DAOTOW010000658 DAOTOW010000000
VERSION     DAOTOW010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70625_length_1443_cov_2.861671"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1107)
                     /locus_tag="P5243_10190"
     CDS             complement(85..1107)
                     /locus_tag="P5243_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRS19864.1"
                     /translation="MGLFSFLTQEIAIDLGTANTIIIQNDKIVVDEPSIVAVDTHSGK
                     PVAIGHKARQMQGKTHQNITVIRPMKDGVIADFNAAEIMIRGMIKMIDKKSRMISPSL
                     KVVICIPSGCTEVELRAVRDSAENAGGREVYMIYEPMAAAIGIGLDVEAPEGNMVVDI
                     GGGTTEIAVIALGGIVCDKSIKTAGDNFTADIQEYMRYQHNIKIGERTAEEIKINVGA
                     VIPDLDDPPADYIVRGPHQMSALPIEIPVSYQEIAYCLDKSIARIEAAILAALEQTPP
                     ELYADIYRRGIYLAGGGALLRGLAKRLEAKHKIKFHIAEDPLHAVARGTGIALKNIDN
                     FQFLIR"
     gene            complement(1122..>1443)
                     /locus_tag="P5243_10195"
     CDS             complement(1122..>1443)
                     /locus_tag="P5243_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HRS19865.1"
                     /translation="AIAIVSQNTDGSYWLTGAGMGQPNRLDSLRWLTIPRFNLKEGVT
                     IEDSVLISDAFFPFRDSVEVANEYNVKYIVQPGGSIRDEDVIAACNEFDIAMVFTGRR
                     HFKH"
BASE COUNT          426 a          319 c          223 g          475 t
ORIGIN      
        1 gttttactat gaagattact aaattcctca tgcttgcata atgtgttttt tagccaatac
       61 atgcttaccg cagcaacata tgtattatcg aattaagaat tggaaattat ctatattttt
      121 aagagctata cctgtacctc gagcaacagc atgcaatgga tcttcggcaa tatggaattt
      181 aatcttatgt tttgcttcca aacgttttgc caaaccacgc aataaggcgc caccaccggc
      241 caaataaatt cctcttcgat agatatcagc atataattca ggtggagttt gctcaagagc
      301 agctaatatt gcagcttcta tacgagcaat agatttatct aaacaatatg caatttcttg
      361 atatgaaacc ggaatttcaa taggtaatgc cgacatttga tgaggcccac gcacaatata
      421 atctgccggt ggatcatcaa gatcgggaat aacggctccc acattaattt taatctcttc
      481 ggcggtacgc tctccaattt ttatattgtg ttgataacgc atatactctt gaatatcggc
      541 tgtaaaatta tcgcctgcgg ttttaataga cttatcacaa actatgcctc caagagctat
      601 aacggcaatt tctgtagttc caccacctat atcaaccacc atattacctt cgggagcctc
      661 tacatctaac cctattccta ttgctgcggc cattggttca tatatcatat acacctctct
      721 acctcctgca ttttcggcag aatcacgtac tgcacgcaat tctacttcgg tacagcccga
      781 aggtatacaa ataaccactt tcaacgatgg cgatatcatg cggcttttct tatcaatcat
      841 ttttatcata ccgcgaatca ttatttctgc agcattaaag tcagctatca ccccatcttt
      901 cataggtctg attacggtaa tattctgatg cgttttacct tgcatttggc gtgctttatg
      961 accaattgct acaggttttc cggaatgcgt atctacagca actatacttg gctcatctac
     1021 aactatttta tcattttgaa taataatagt atttgctgtt cctaagtcaa ttgctatctc
     1081 ttgcgtgaga aatgaaaata aacccatgtg tatatcgtat tttaatgttt aaaatgtctt
     1141 cgtccggtaa ataccattgc tatatcaaat tcattacatg ctgcaattac atcttcatcg
     1201 cgaatactac ctcccggttg tactatgtat tttacgttat attcattagc aacctctaca
     1261 ctatctctaa atggaaaaaa tgcatctgat ataagcactg aatcttcaat tgttactccc
     1321 tctttgagat taaaacgagg tatagttaac caacgtaaac tatctaatct gtttggttgt
     1381 cccataccag cacctgttaa ccaatatgaa ccatcggtat tttgagacac aatagctata
     1441 gca
//
LOCUS       DAOTOW010000659         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70748_length_1441_cov_3.966089, whole genome shotgun sequence.
ACCESSION   DAOTOW010000659 DAOTOW010000000
VERSION     DAOTOW010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70748_length_1441_cov_3.966089"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="P5243_10200"
     CDS             <1..534
                     /locus_tag="P5243_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118304733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRS19866.1"
                     /translation="ETGVHRLVRISPFDSGARRHTSFASVDVAPMIDDTIEIVVNPAD
                     IEWDTYRSSGAGGQNVNKVETAVRLKHKPTGIVIENSETRSQLQNREKAMTLLKSQLY
                     ELEIRKRQEEQAARNGEKLKIEWGSQIRNYVLHPYKLVKDVRTNYETSNTQAVLDGDL
                     NPFIKEYLMMFVRKEEQ"
     gene            536..1408
                     /locus_tag="P5243_10205"
     CDS             536..1408
                     /locus_tag="P5243_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRS19867.1"
                     /translation="MAAHYLVLILISALAHSFYNFLVHKSGGNRLFLLLMFVVAAISA
                     SVVYGFNFEFFTFSFKTFAIIYAASLFYILYQVFVSKSYELGEISRLYPLSVLSPVLV
                     PIWAVLFLHEQLSVSIVIGICLSTAGAMIMKQKSLRSMEFADLFTKRNLYAGAGYALT
                     ASLMYSFGSVLDKFSVGLFNLIPYLWLLLTCMTLNLFIYTLLFENKVFASYKTIAWNR
                     LIIAGVAAYISFFTFRAALQHVPVSVAVPIRTSSIVFALLMGVLFVGEKINTIKIIGI
                     TTIIIGIVIINISL"
BASE COUNT          432 a          219 c          271 g          519 t
ORIGIN      
        1 gaaaccggag ttcatcgttt ggttcgtatt tcaccgttcg actcgggagc tcgtcgtcat
       61 acttcttttg cttcggtaga tgtagcacca atgattgacg atactattga aattgttgta
      121 aatcctgccg atatagaatg ggacacgtat cgttcgagtg gtgctggcgg gcaaaatgta
      181 aataaagttg aaaccgcagt aaggttaaaa cataaaccta ccggaatagt tatagaaaat
      241 tcagaaactc gttctcaatt gcaaaaccgt gaaaaagcaa tgactttgct taaatcacaa
      301 ttgtatgaac ttgaaattcg taaacgccaa gaagaacaag ctgctcgcaa tggtgaaaaa
      361 cttaaaattg aatgggggtc tcaaatacgc aattatgtat tgcacccata taaattggta
      421 aaagatgtga gaacgaatta tgaaacgtct aatacacaag ctgttttaga tggtgattta
      481 aatcctttta taaaagaata tttaatgatg tttgtacgaa aagaggaaca ataatatggc
      541 agctcactat cttgtgttaa ttctcatttc ggcattggca cattcatttt acaatttttt
      601 ggtacataaa tcaggtggca atagactgtt tttgttgctt atgtttgttg tagctgctat
      661 ttctgcttct gttgtgtatg gttttaattt tgaatttttt acattttctt ttaaaacatt
      721 cgcaattatt tatgcagcgt cgttatttta tatattgtat caggtatttg tatcaaaatc
      781 atatgaactt ggcgaaatat cacgattgta tccactttct gtattgagcc cggtgttggt
      841 accaatatgg gctgtattgt ttttgcacga acaactaagt gtaagtattg ttataggtat
      901 atgtttgtca acagcaggag ccatgattat gaaacaaaaa tcgttacgtt ccatggaatt
      961 tgccgattta tttactaaac gaaatttata tgccggggca ggctatgctc tcacagcatc
     1021 acttatgtat tcgtttggtt cagtcctcga taaattttct gtcggtttat ttaatcttat
     1081 tccctattta tggcttttac ttacatgtat gactcttaat ttatttatat atacgctttt
     1141 gtttgaaaat aaagtttttg cctcatataa aactatagct tggaatcgtt tgataatagc
     1201 aggtgtagct gcatatattt ctttttttac gtttcgagca gctttacagc atgtgcctgt
     1261 gtcggttgcg gtacctatta gaacttcgtc aattgtattt gctctgctta tgggagtttt
     1321 gtttgtaggc gaaaaaatca atactataaa aattattggt attactacta taatcattgg
     1381 aattgtaatt attaatatat cattataaca tggcatttga aaaggaaatt aatagacgac
     1441 g
//
LOCUS       DAOTOW010000660         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70754_length_1441_cov_3.114719, whole genome shotgun sequence.
ACCESSION   DAOTOW010000660 DAOTOW010000000
VERSION     DAOTOW010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70754_length_1441_cov_3.114719"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1014
                     /locus_tag="P5243_10210"
     CDS             124..1014
                     /locus_tag="P5243_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19868.1"
                     /translation="MIRCIPCNKIDKVLWDKTVSESKHPVLYAFSWYLDIVSPQWKAL
                     IYGNYEYIMPLPVKRKYGIPYIVQPHITQQLGIFSKHDITPEILKTFEKSLFSFFSVR
                     YSSNIQFQTTKARIKQRVNYILPLQKSYEELYSNFSENCVRNIKKSQKNCIQVQLVSL
                     DFFITHFKELSLFTIPEPIWQTIQTVLSQAQNSQSLYIYSAFFEHKVCALAAFITSNN
                     SMYYLVPGANSVARKYGASYAIVNACIKEHCLQYAKLDFEGSEIAGIAQFYASFGAIV
                     QPYYYISKHSIPGIYSFLKK"
     gene            1093..>1441
                     /locus_tag="P5243_10215"
     CDS             1093..>1441
                     /locus_tag="P5243_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS19869.1"
                     /translation="MKYCVVCIVLVLSFTCYSQDNDFQIRTFTQEHGLPQNSITAITQ
                     DAQGILWLATPIGFYYYNGYRFSKLNDSRVSDVQALLYSSDSVLYIASSSGLYVLNPI
                     NYSLYPIIRNKKIT"
BASE COUNT          512 a          212 c          215 g          502 t
ORIGIN      
        1 gactgtcgcg tccaacgaat ttatgatcaa atgaaatgaa aaaaaacaag aaaaaggccg
       61 aaatgcgaaa taatagaaaa aaaccaaata aaatacgaaa actcttttta caaatatagt
      121 atcatgattc ggtgtattcc ttgtaacaaa atagataaag tactttggga caaaacggta
      181 tctgaatcaa aacaccctgt attatatgct ttttcatggt atttagatat agtttcgcct
      241 cagtggaagg cacttatata tggtaactat gaatatatca tgcctctgcc tgttaaacga
      301 aaatacggta taccttatat tgttcaacct catataacac aacaattagg tattttttca
      361 aaacatgaca ttacacccga aattttgaaa acatttgaaa agagtttgtt ttcatttttt
      421 agtgttcgat atagcagcaa tattcaattt cagaccacca aagcacgcat aaaacagcgg
      481 gtaaattata tattgcctct tcaaaaatcg tatgaagaat tatattctaa tttttcggaa
      541 aattgtgtaa gaaacataaa aaaatcacaa aaaaattgta ttcaagtaca attagtttca
      601 ttagattttt ttataactca tttcaaagaa ttgagtttgt ttaccatacc tgaacctatt
      661 tggcaaacta tacaaactgt tcttagtcaa gcacagaatt cacaatcact gtatatatat
      721 agtgcttttt ttgaacataa agtttgtgcc ttggccgcat ttattacaag taacaattca
      781 atgtattatt tagttcccgg agctaattct gtagcacgta aatatggtgc ttcgtatgca
      841 attgttaatg catgtattaa agaacattgt ttacaatatg caaaacttga ttttgaaggc
      901 tcggaaatag caggtatagc acagttttat gcaagttttg gagctattgt acaaccgtat
      961 tattatattt caaaacattc tataccaggt atttatagtt ttttgaaaaa ataatagtat
     1021 ttttattttc ataattatag agggaatttg agaaatattc tgtcttgata caggtttaga
     1081 atcatatggt atatgaaata ttgtgttgtt tgtatagttc ttgttttatc attcacttgt
     1141 tattcacaag ataatgattt tcaaattcgt acatttacac aagaacatgg cttaccacaa
     1201 aattctataa ccgccataac acaagatgct caaggaattt tatggttggc tactcctata
     1261 gggttttatt attacaatgg atatcgattt tctaaattaa atgattctcg agtgtccgat
     1321 gtgcaggctc ttttatattc aagcgatagt gtactatata ttgcttcaag cagtgggctg
     1381 tatgtgctta atcctataaa ttattcttta tatccaatta taagaaataa aaaaataact
     1441 a
//
LOCUS       DAOTOW010000661         1439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_70904_length_1439_cov_2.890896, whole genome shotgun sequence.
ACCESSION   DAOTOW010000661 DAOTOW010000000
VERSION     DAOTOW010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70904_length_1439_cov_2.890896"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="P5243_10220"
     CDS             <1..297
                     /locus_tag="P5243_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRS19870.1"
                     /translation="EGKTRLESMVQTTDGFELAEIDLKLRGPGDIEGTQQSGLPITLK
                     LANLAKDGNIIQIARKKAFEIIEKDPTLQLPENNILYKGLQAKNIQKISWRDIS"
     gene            complement(398..733)
                     /locus_tag="P5243_10225"
     CDS             complement(398..733)
                     /locus_tag="P5243_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRS19871.1"
                     /translation="MKHIELSPEKLFYTIGEVADMFEVNTSLIRFWEKEFSVIKPKKN
                     AKGNRLFTAQDIENFKLIYFYVKELGMTLKGAQKKIAENKQAAMSTMSIADRLTGIKA
                     KLLEIHGNL"
     gene            complement(817..>1439)
                     /gene="miaA"
                     /locus_tag="P5243_10230"
     CDS             complement(817..>1439)
                     /gene="miaA"
                     /locus_tag="P5243_10230"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRS19872.1"
                     /translation="LCGGSGMYIDAVCNGIDDIPDIDIHIRETVTNQYATEGIESLRR
                     TLLKLDPEYYNRVDLKNPARLIRAIEVCLQTGKTYTSVRTNSKKQRHFTIIKIALNIP
                     REQLYSKINHRVDYMVQNGLEQEVMQVIQYRNHTALKTVGYREFFEYVDGVISKERAI
                     EAIKQNTRNYAKRQISWLKRDSAYTWFNPADILEIIAYINQNSIYK"
BASE COUNT          465 a          242 c          204 g          528 t
ORIGIN      
        1 gaaggtaaaa cccgattaga aagtatggtt caaaccaccg acggatttga acttgccgaa
       61 attgatttaa aactccgagg acctggtgat atagaaggaa ctcaacaaag tggattacca
      121 attactctaa aactggcaaa tttggcaaaa gacggaaata taattcaaat tgcccgtaaa
      181 aaagcttttg aaattattga aaaagaccca actttacaac ttcccgaaaa caacatatta
      241 tacaagggat tacaagcaaa aaatattcaa aaaatttcgt ggagagatat cagttagcac
      301 tatgaattaa tacctttgaa tacttgttat ctgtctattt tttgggtata ttatatatct
      361 gctctgtgta actcagagct gtttgtagta agaaatatta caaatttcca tgaatctcaa
      421 gcaattttgc ttttattccg gtcaatctat cggctatact cattgttgac atagcagcct
      481 gtttattttc agctattttt ttttgagcac ctttcaaagt catgcccaac tcttttacat
      541 agaagtatat aagcttgaag ttttcaatgt cttgtgcagt aaataagcga ttgccttttg
      601 catttttttt aggttttatc acactaaatt ctttttccca aaaacgtatt aacgatgtgt
      661 taacctcaaa catatcagca acttcgccta ttgtgtaaaa taatttttcg ggtgataatt
      721 ctatgtgttt catacctttt tgtttttgaa tgtatcaact ataaatacaa gtttttcaaa
      781 tatataacaa ataattaaaa tataaaaaaa cagaaactat ttatatatac tattttgatt
      841 aatataggca attatttcca aaatatctgc cggattaaac catgtatatg cagaatcgcg
      901 tttaagccat gaaatttggc gttttgcata atttcttgta ttttgtttta ttgcttctat
      961 tgctctttct tttgaaataa ctccatcaac atattcaaaa aactctctat acccaactgt
     1021 ttttaaagca gtatgatttc tatattgtat tacttgcatt acttcttgtt ccagaccatt
     1081 ttgaaccata taatctaccc tatggttaat ttttgaatat aattgttctc ttggtatatt
     1141 tagtgcaatt ttaattatag taaaatgtct ttgttttttg gaattggtac gcacacttgt
     1201 ataggttttt cctgtttgca aacacacttc aattgctctt ataagccgag ccggattttt
     1261 taaatctact ctattataat attccggatc aagtttcaat aatgtgcgtc taagactttc
     1321 aatgccttct gttgcatatt gatttgtaac ggtttcacga atatgaatgt caatatcggg
     1381 aatatcgtca attccattac atacagcatc aatatacatt ccagagccgc cacagagta
//
LOCUS       DAOTOW010000662         1436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_71148_length_1436_cov_2.832730, whole genome shotgun sequence.
ACCESSION   DAOTOW010000662 DAOTOW010000000
VERSION     DAOTOW010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71148_length_1436_cov_2.832730"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="P5243_10235"
     CDS             complement(<1..1222)
                     /locus_tag="P5243_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRS19873.1"
                     /translation="MKHIINLKGNKAYSWYSHENVLCIGYFFDTSHTLFSDFEAAKAI
                     IDMRKTMSFEDILSHIQGIFTIIEISDNHISIITDSINYFPVFYLYYNQIWHITDSWN
                     TLIETKSEININTQALIEYECAGFVLDNQTLDRNILKTKAWEQLTITEKGEYIRTKTG
                     NFLPKSFLFEPIEQLQQKAYNVYNNVSKRLIDFLQNRTAVVPLSGGFDSRLIVSLLYA
                     AQYKNVICFTYGKMNHEVPLSKQVAETLGYPWYFIDYTTIQLESLENSDIQTYLQSAA
                     QGYSMPYLQEYYAVKELIRKQLIPSDSVFLPGHSGDYLGGSYINKTIEAQLIFEEIPA
                     HIESKYFYFKKKSRYEKIHLQERIALTLDKNKHTIIQNKYNPIIEDWDIQEKLSKFIF
                     HSSYVFNYFGYQHYF"
     gene            complement(1219..>1436)
                     /locus_tag="P5243_10240"
     CDS             complement(1219..>1436)
                     /locus_tag="P5243_10240"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRS19874.1"
                     /translation="GLGITIIGSYILIPSLGITGACITTVAANVISSIIVIYKFYTNT
                     SFSLSDFIITKSEITMLYSKILSKIHS"
BASE COUNT          489 a          206 c          209 g          532 t
ORIGIN      
        1 gaaaataatg ttggtatcca aaataattaa acacatatga tgagtgaaaa ataaattttg
       61 atagtttttc ttgtatatcc caatcttcaa ttattggatt atatttattc tgtatgatag
      121 tgtgtttgtt tttgtccaaa gtcagagcaa ttcgttcttg caaatgaatt ttctcatatc
      181 tgcttttctt tttaaaataa aaatactttg attcaatatg tgccggtatt tcttcaaaaa
      241 tcaattgagc ttcaatagtt ttgttaatat aactgccacc taaataatca cctgaatgcc
      301 ccggcaaaaa gactgaatct gatgggatta attgtttgcg aataagttct tttactgcgt
      361 agtattcttg tagatagggc atactgtatc cttgagctgc actctgtaaa tatgtctgta
      421 tatcagaatt ctcaagtgat tctaattgaa ttgtagtata atcaataaaa taccaaggat
      481 accctaatgt ttcagcaacc tgtttactta aaggaacttc atgattcatt ttaccatacg
      541 taaagcatat tacgttttta tattgagcag catataatag gcttactata agtcgagaat
      601 cgaaccctcc actaagtgga accacagcag tacgattctg taaaaaatca ataaggcgtt
      661 tgcttacatt attatataca ttgtaggctt tttgctgtaa ttgctctatg ggttcaaaca
      721 gaaaagattt tggtaaaaaa ttacctgttt ttgtacgtat atactctcct ttttctgtaa
      781 tagtaagttg ttcccatgct tttgttttta aaatatttct gtctaaagtt tggttgtcga
      841 gtacaaatcc ggcacattca tattctataa gagcttgggt atttatattg atttcacttt
      901 ttgtttcaat taacgtattc catgagtctg taatatgcca aatttgattg taatacaaat
      961 aaaaaacggg aaaataatta atactatcag taattattga aatatgatta tcagagattt
     1021 caataattgt aaaaatacct tgaatatgag ataaaatatc ttcaaaactc atagtctttc
     1081 tcatatctat aattgctttt gcagcctcaa aatcagaaaa taaagtatgt gacgtatcaa
     1141 aaaaataacc gatacatagt acattttcat gcgaatacca tgaatatgct ttgttacctt
     1201 taagattaat tatatgtttc atgaatgtat ttttgaaagt attttggaat acagcatagt
     1261 aatttctgat tttgtaatga taaaatcact caatgaaaat gatgtattgg tataaaattt
     1321 ataaatcaca ataattgaag aaattacatt agccgcaact gttgtaatac atgctcctgt
     1381 tattcctaat gatggtatga gtatatatga accaattatt gtaattccta atccaa
//
LOCUS       DAOTOW010000663         1425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_71969_length_1425_cov_3.556204, whole genome shotgun sequence.
ACCESSION   DAOTOW010000663 DAOTOW010000000
VERSION     DAOTOW010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71969_length_1425_cov_3.556204"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1371
                     /locus_tag="P5243_10245"
     CDS             235..1371
                     /locus_tag="P5243_10245"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRS19875.1"
                     /translation="MTIKDKLNINRHLFYETSHSPSLFDIVGNEMIEKITDFCFIANP
                     YYPNKELITKLQERLPILIKSYPSSNPTVSQKHLSNVLHIDPEYIIIGNGATELITII
                     QKECINNLAIPVPTFSEYTEKIRNQQQSKLFYLPAENNYQLDLNEYAQWIIQNSISSA
                     LVINPGNPTGQLHSLEEMFSFLHATKHLQLIIIDESFIDFAGIEIPSLLPYVHEFQNI
                     VIVRSMSKHCGIPGLRLGYCVTANTDLLHKIRASIPVWNINTIAEYFLTQLYITDEVY
                     HEARKQVISDVTLLYNELSQIPGFYVYPTGANFVLIRIDIGVSAGELQHMLLEDYYLY
                     VRDCSNKIGLDNYHIRVASQGKEKDALLIHALQQISKKYIHHVS"
BASE COUNT          550 a          200 c          193 g          482 t
ORIGIN      
        1 aattatatct aaaaaagata actaaactgt cctattaata gggagtatta tagtattttc
       61 gtgttttttt gtatgaattc tgcgtaaatt tgtttgcgta atttactgag aagcatgcta
      121 caataattgg tttaatctct aatgtttcac tactaaaaaa aatactttta ctaatgtaaa
      181 taagcataca aataatttat atttttggta gaatcaaatt aacattataa taacgtgaca
      241 ataaaagata aactaaatat aaaccgacat ctgttttatg agacatctca ttctccttca
      301 ttgtttgata ttgtaggaaa tgaaatgatt gaaaaaatta ccgatttttg ttttattgca
      361 aacccatatt accccaacaa agaactaatt acaaaattac aagagcgatt gcctattctt
      421 attaaatcat atccttccag caatccaact gtttcacaaa aacacttaag taatgtatta
      481 catattgatc ctgaatatat tataataggt aatggagcaa cagaattaat tacaattatt
      541 caaaaagaat gtattaacaa tttagcaata cctgtaccta catttagtga atacaccgaa
      601 aaaattagaa atcaacaaca atcaaaattg ttttatttac ctgcagaaaa taattatcaa
      661 ttagacctta atgaatatgc tcaatggatt attcagaatt ctatatcaag tgcacttgtt
      721 ataaacccgg gtaaccctac aggtcaatta cattcattag aggaaatgtt ttctttttta
      781 cacgcaacca aacacttaca gttaataatt atagatgaat catttataga ttttgcagga
      841 atagaaatac ctagtttatt gccatatgta catgaattcc aaaatattgt aatagtacgt
      901 tctatgagta aacattgtgg tattcccggt ttacgtttag ggtattgtgt tacagcaaat
      961 actgatttat tacataaaat cagagcatca ataccggtat ggaatattaa tacaatagcc
     1021 gaatatttct taacacaatt atatataaca gacgaagtat accatgaagc tcgtaaacaa
     1081 gtaatttctg atgtaacctt gttatataat gaattatctc aaattcccgg tttctatgtg
     1141 tatcctactg gagcaaattt tgttttaatt cgaatagata taggtgtaag cgcaggtgaa
     1201 ttgcagcata tgcttcttga agattattac ttatatgtta gggattgtag taataaaata
     1261 ggattagata actatcatat tcgcgtagct tcacaaggaa aagaaaaaga tgcattatta
     1321 atacatgcat tacaacaaat ttcaaaaaaa tatattcatc atgtcagtta aaaaagtact
     1381 tatagtaagt aggagatata ccagaaaaaa taaattaata aattg
//
LOCUS       DAOTOW010000664         1425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_71997_length_1425_cov_2.397810, whole genome shotgun sequence.
ACCESSION   DAOTOW010000664 DAOTOW010000000
VERSION     DAOTOW010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71997_length_1425_cov_2.397810"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="P5243_10250"
     CDS             complement(<1..1103)
                     /locus_tag="P5243_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRS19876.1"
                     /translation="MLVETAFKSGVWTDTIEVKDFVAQNITPYFGTHEFLQPPTHRTI
                     HLWNICKQALAEERQRNGLRAVDTEIVSSMTAFKAGYIDKEYEVIVGLQTDELLKRAM
                     KPFGGFKLVQRALAEHGLKPSKTVTDYFEKYAKTHNDGVFDVYSDEIKKFRTFGIITG
                     LPDNYARGRIIGDYRRVALYGISRLIEAKKQDLSSIVGPMTDEVIRLREEVASQIKAL
                     EEMIEMGELYGLDLSRPAENAKEAVQWTYMAYLAAVKEQDGAAMSLGSVSSFLDIYIE
                     RDLQLGIITESEAQEYIDQFVMKLRMVRHLRMESYNQIFAGDPTWVTESIGGMFIDGR
                     TKVTKTSFRFLHTLYNLGPSPEPNITILWSKHLP"
     gene            complement(1204..>1425)
                     /locus_tag="P5243_10255"
     CDS             complement(1204..>1425)
                     /locus_tag="P5243_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19877.1"
                     /translation="IFYNKSKNYIEFKLEDKIIKNIDFIEIISLQGQVIYRQNFYSPT
                     IQNIALPKGAFYVYVQHGKTFTTQQFICN"
BASE COUNT          460 a          263 c          229 g          473 t
ORIGIN      
        1 ggtaagtgtt tagaccataa tatggtaata tttggttcag gcgaaggacc taaattatac
       61 aaggtatgta aaaaacggaa cgatgtttta gtaacttttg ttctgccatc gataaacatg
      121 cctccaatag attcggttac ccacgtagga tcgccggcaa aaatttgatt atatgattcc
      181 attcgcaagt ggcgaaccat gcgaagtttc attacaaatt ggtcaatata ttcttgtgct
      241 tctgattcgg taataattcc taattgtaag tcgcgttcta tataaatatc aagaaacgac
      301 gaaacactac caagcgacat tgcagcaccg tcttgttctt taacggcagc aagatatgcc
      361 atataggtcc actgtacagc ttctttggca ttttcggcag ggcgagataa atcaagacca
      421 tataactccc ccatttcaat catttcttct aatgccttaa tttgagaagc aacctcttcg
      481 cgcaatcgaa taacctcatc ggtcataggt cctactatgg aagacaaatc ttgtttttta
      541 gcttctataa gcctactaat accatataat gctacacgac gataatcgcc tataatccta
      601 ccgcgtgcat aattatcggg caatccggtg ataataccaa acgttctgaa ttttttaatc
      661 tcatcggaat atacatcaaa caccccatca ttatgagttt ttgcatattt ttcgaaataa
      721 tcggtaacag ttttacttgg ttttagtccg tgctctgcta aagctcgttg aaccaatttg
      781 aaccccccaa agggtttcat ggcacgtttc agtaattcat cggtttgtaa accaactatt
      841 acttcatatt ctttatcaat atagccggct ttaaaagcag tcatacttga aactatttca
      901 gtatcaactg cacgtaaacc attgcgttgc cgctcttcgg caagagcttg tttacaaata
      961 ttccaaagat gaattgtacg atgagttggt ggctgcaaaa attcgtgggt tccaaaatat
     1021 ggtgtaatat tttgagcaac aaagtctttt acctcaattg tgtctgtcca tacccccgat
     1081 ttaaaagcag tttctaccag cattcaattc aatttaaatt cgggttaaat atacagtgat
     1141 atttattgtt gaattaacaa ttacgaaaat aaataataac aatttgttga caataaataa
     1201 tgattaatta caaatgaatt gttgagttgt aaatgttttt ccatgttgca cataaacata
     1261 aaatgctcct ttggggagag ctatattttg aatagtcgga gaataaaaat tctgacgata
     1321 tataacttgc ccttgtaaag aaataatttc aataaaatca atgtttttaa taattttatc
     1381 ttctaattta aattctatat aatttttaga tttattataa aaaat
//
LOCUS       DAOTOW010000665         1425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_72015_length_1425_cov_1.972263, whole genome shotgun sequence.
ACCESSION   DAOTOW010000665 DAOTOW010000000
VERSION     DAOTOW010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72015_length_1425_cov_1.972263"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="P5243_10260"
     CDS             <1..241
                     /locus_tag="P5243_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19878.1"
                     /translation="TKITINGKPVVFTFKEKKSQDAAEWYYFISKEHTANCNISVENN
                     LLCNIYHDQKNLVIISCNSQEKGFSLDYSNRTCIY"
     gene            245..>1425
                     /locus_tag="P5243_10265"
     CDS             245..>1425
                     /locus_tag="P5243_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19879.1"
                     /translation="MKKIITIIFSILYATLLYATHNRAGEITYRQISAYTYEITLITY
                     TYTPSYANETRDVLPIDWGDGTMSNIPRIQIEYLPDNYTRNTYRMTHTFPGPGTFRIV
                     MEDPNRNQGVANIFDSVNIPFSISTTLRIDAILGNNSTPILLNPPVDKAALGQRFVHN
                     PAAYDPDGDSLSYALTVCTGQNGEPIPNYTFPQASNQFYVDSISGDLVWDAPVALGIY
                     NVAMVVKEWRKGVLIGQVVRDIQIEVFQSNNNTPMIDPIENICIRANDTVRFTVKAHD
                     IDNNYITLSASGGPLEKVSSKATFTSVSGFGTIQSEFLWTPSCNEIREQPYTIVFKVV
                     DAGNEISLAGYKYVTIQVIGHAPVQLQATSKSFAIDVQWADTICSNQQSYSIYRSTLS
                     QP"
BASE COUNT          494 a          252 c          242 g          437 t
ORIGIN      
        1 tacaaaaatt actataaacg gtaaaccggt agtgtttaca tttaaagaaa aaaaatcaca
       61 agacgctgct gaatggtact attttatatc aaaggagcat actgcaaatt gtaatatttc
      121 ggttgaaaat aatttattgt gtaacatata ccatgatcaa aaaaatttag taataatatc
      181 atgtaattcg caagaaaagg gttttagcct tgattattca aacagaacct gtatctatta
      241 aacaatgaaa aaaatcatta ccataatatt tagtatactg tatgcaactc tgttgtatgc
      301 aacgcataac agagccggtg aaataacgta tcggcaaata tcggcttata cttatgaaat
      361 tacacttata acatatacat acactccaag ctatgccaat gaaacacgcg atgtgcttcc
      421 tattgattgg ggcgatggaa ctatgagtaa tatacctcga atacaaattg aatatttacc
      481 tgataactat accagaaata cttataggat gactcataca tttcccggac ctggtacatt
      541 tcgtattgta atggaagatc ctaataggaa tcaaggtgtt gcaaatattt ttgattcagt
      601 aaatattcct ttttcaattt ctactacatt gcgaatagat gcaatattag gtaataacag
      661 taccccaatt ctactaaacc ctcctgttga caaagctgca ttgggccaac gatttgtaca
      721 taaccctgca gcatatgacc ccgatggtga tagcttatcg tatgctctta ctgtatgtac
      781 cgggcaaaac ggagaaccta ttccaaatta tacatttccg caagccagta atcaattcta
      841 tgtcgattct atttcgggtg atttggtttg ggatgctcct gttgcattag gtatatacaa
      901 tgtagctatg gtagtaaaag agtggcgaaa aggcgttctc ataggtcaag tagtacgtga
      961 tattcaaata gaagtatttc aatcaaataa taatactcct atgattgacc ctatagaaaa
     1021 catttgcata cgtgctaatg atactgtaag atttacagta aaagctcatg atattgacaa
     1081 caactacata actttaagtg caagtggtgg cccattagaa aaagtaagta gtaaagctac
     1141 ctttacttct gtttcgggat ttggcactat acaatccgaa tttttgtgga ccccctcatg
     1201 caatgaaatt cgcgaacaac cctataccat tgtatttaaa gttgtagatg caggcaatga
     1261 aatttcgctt gcaggttata aatatgttac cattcaagta atagggcatg ctccggtgca
     1321 attacaagca acatctaaaa gttttgctat agatgtgcaa tgggctgaca caatttgttc
     1381 aaatcaacaa tcatatagta tttatagatc aacactatct caacc
//
LOCUS       DAOTOW010000666         1421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_72289_length_1421_cov_3.132504, whole genome shotgun sequence.
ACCESSION   DAOTOW010000666 DAOTOW010000000
VERSION     DAOTOW010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72289_length_1421_cov_3.132504"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="P5243_10270"
     CDS             <1..276
                     /locus_tag="P5243_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HRS19880.1"
                     /translation="SPLHDGAVIIIGNKIRAAKCVLPTTTKMDLPIEYGMRHRSAIGM
                     SEETNTLVIVVSEQTGAISYCLSGRIKRIEDAQVLNSVIRRKMRGDK"
     gene            276..>1421
                     /locus_tag="P5243_10275"
     CDS             276..>1421
                     /locus_tag="P5243_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRS19881.1"
                     /translation="MNSQLPLAERMRPKHIDDYVGQTHLVAKGAVLRNAIESGRLPSI
                     ILWGPPGVGKTTLAHIISQTLQRPFYTLSAISSGVKDVRDVIEKARKAQFFNTQNPIV
                     FIDEIHRFSKSQQDSLLGAVEDGTITLIGATTENPSFEVISPLLSRCQVYVLQSLDSS
                     ELKTLLHKALECDIVLKNIHITIEEDDALLLFSGGDARKLYNVLEIVIHAQPVGETIV
                     ITNELVTACLQKNIALYDKGGEQHYDVISAFIKSIRGSDPNAAVYWLARMLEGGEDIA
                     FIARRMIISAAEDIGLANPNALLLAQACFESVKVIGMPESRIILSETAIYLATSPKSN
                     STYLAIDDAIALVQKTGSLPVPLHLRNAPTKLMKQLQYGKNYKYSHEY"
BASE COUNT          475 a          226 c          283 g          437 t
ORIGIN      
        1 agtcctttgc atgatggagc cgttataatc ataggaaata aaattcgtgc tgccaaatgt
       61 gtgttgccta caacaactaa gatggattta ccaatagaat atggaatgcg tcatcgttct
      121 gccataggta tgagtgaaga aaccaataca ttggtaatag tagtatcgga acaaaccggt
      181 gctatatcgt attgtttaag tggaagaatt aagcgaatag aggatgctca agtattgaat
      241 tctgttattc gcagaaaaat gagaggtgat aaataatgaa ttcacaattg cctttagcag
      301 aacgaatgcg tcccaaacat attgatgatt atgttgggca aactcattta gtcgcaaaag
      361 gagctgtttt acggaatgca atagaatcgg gtagactgcc ttctattatt ttatggggac
      421 cacccggagt tggtaaaact accttagctc atattatttc acaaacgctg caacgaccat
      481 tctatacttt aagtgcaata agttccggtg ttaaagatgt gcgtgatgtg attgaaaaag
      541 cacgaaaagc acaatttttt aatactcaaa atccaattgt atttattgac gaaattcata
      601 gatttagtaa atcgcaacaa gattctttat tgggtgctgt tgaagatggt actataacct
      661 taataggtgc aacaacagaa aatccctcat ttgaagtaat ttcaccattg ttgtcgcgat
      721 gccaagtata tgtgttgcaa tcgttagatt cttcagaatt aaaaacgttg ttgcataagg
      781 cattagaatg tgatattgtg ttaaaaaaca tacacataac tattgaagaa gatgatgcat
      841 tattgttgtt ttcgggtggc gatgctcgta aattgtataa tgtattagaa attgtaatac
      901 atgcacaacc ggtaggagaa acaatagtta taacaaatga attagttaca gcttgtttac
      961 aaaaaaatat tgccttatac gataaaggag gtgaacaaca ctatgatgta atttctgcat
     1021 ttataaaatc tattcgcggg agtgacccta atgctgcagt gtattggctt gcacgtatgc
     1081 tcgaaggagg tgaagatatt gcgtttattg cccgtagaat gattatttct gcagctgaag
     1141 atataggttt ggccaatccc aatgccttat tacttgcaca agcttgtttt gaatcggtaa
     1201 aggtgatagg aatgcccgaa tcacgcatta tattatcaga aactgccata tacttagcta
     1261 cttcacctaa aagtaattca acctatttgg ctatagatga tgctattgca ttagttcaaa
     1321 aaacaggttc attacccgtg ccgttgcatt tacgaaatgc tcctacaaaa cttatgaaac
     1381 agttgcagta tggtaagaac tataaatatt cacatgaata t
//
LOCUS       DAOTOW010000667         1420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_72363_length_1420_cov_3.125275, whole genome shotgun sequence.
ACCESSION   DAOTOW010000667 DAOTOW010000000
VERSION     DAOTOW010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72363_length_1420_cov_3.125275"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..>1420
                     /locus_tag="P5243_10280"
     CDS             277..>1420
                     /locus_tag="P5243_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRS19882.1"
                     /translation="MADKRKFMTCDGNYAAAYVSYAFTETAGIYPITPSSDMAEHTDQ
                     FSAFGKKNIFGDTVKVVEMQSEAGAAGTVHGALQAGALTTTYTASQGLLLMLPNMYKI
                     AGELLPAVFHVSARAIAAQALSIFGDHQDVYSARMTGFSMLCAGSVQEVMDLGAVAHL
                     SAIKSSVPVLHFFDGFRTSHEIQKIQAWEYSELAEMVDMEAVQKFRDKAINPEHPVTR
                     GTAQNPDVYFQAREASNPFYMAVPEVVEHYMNEVNKRTGRNYQLFNYYGASDAEYVII
                     AMGSVSETIKETIDYLVAKGEKVGFLHVHLYRPFSVKHFLGALPKSVKKIAVLDRTKE
                     PGSIGEPLYLDVRAAFYGVENAPVIVGGRYGLSSKDTTPRQIVAVFT"
BASE COUNT          459 a          252 c          272 g          437 t
ORIGIN      
        1 ctaatatctc atatctaata tctcaaatct tataattaaa aattaccctt ataccgttcg
       61 ccttaaaccc tttttttacc acagatttca ttgcgataca caaagcagat ttaactcaaa
      121 actcaaaact ttgtaaaatt ctgtaagcat tttcacgcaa aaacagacaa aaggtaactt
      181 attaagaaaa tgttattaat tttcataaaa aagataagta ttttttttat tttagccacc
      241 tcaataaata aaaatagaaa taaaaattaa ttcattatgg cagacaaaag gaagtttatg
      301 acatgtgatg gtaactatgc agcggcgtat gtatcgtatg catttaccga aacagcaggt
      361 atttacccta ttacgccgtc gtctgatatg gcagaacata ctgaccaatt ttcggcattc
      421 ggcaaaaaaa atatatttgg agatacagta aaagttgttg aaatgcaatc ggaagccggt
      481 gctgccggta ctgttcacgg tgcattacaa gcaggagcgt taaccactac atatacggca
      541 tcacaaggtt tattacttat gcttcctaat atgtacaaaa ttgcaggaga attgcttcct
      601 gctgtattcc acgtaagtgc tcgagctatt gcagctcagg cattgtcaat atttggtgac
      661 catcaggacg tgtattctgc gcgtatgaca ggtttctcta tgttgtgtgc aggtagtgtt
      721 caagaagtta tggatttagg tgcggttgct cacttatcgg ctattaaaag tagtgtacct
      781 gttttacact ttttcgatgg attccgtaca tcgcacgaaa ttcaaaaaat tcaggcttgg
      841 gaatacagtg aattagctga aatggttgac atggaagctg ttcaaaaatt ccgtgataaa
      901 gctattaatc ccgaacatcc ggtaacacgt ggtactgcac aaaatcctga tgtgtatttt
      961 caagctcgcg aggcatcaaa tccattttat atggcagtgc cagaagtagt tgaacactat
     1021 atgaacgaag taaataaacg taccggtcgt aactatcaat tatttaatta ctacggtgct
     1081 tctgatgccg aatatgtaat tattgcaatg ggttcggtaa gtgaaactat taaagaaact
     1141 atagattatt tggtagcaaa aggcgaaaaa gtaggtttct tgcatgtgca tttatatcgt
     1201 ccattctctg tgaaacactt cttgggtgca cttccaaaat cggtgaaaaa aattgccgta
     1261 ttagacagaa caaaagaacc gggatctata ggcgagccat tatatcttga tgtacgtgca
     1321 gctttctatg gtgttgaaaa tgcaccggta attgttggcg gacgttacgg tttatcatca
     1381 aaagatacta ctcctcgtca aatagttgct gtatttacaa
//
LOCUS       DAOTOW010000668         1416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_72650_length_1416_cov_4.902278, whole genome shotgun sequence.
ACCESSION   DAOTOW010000668 DAOTOW010000000
VERSION     DAOTOW010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72650_length_1416_cov_4.902278"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..553
                     /gene="ybeY"
                     /locus_tag="P5243_10285"
     CDS             134..553
                     /gene="ybeY"
                     /locus_tag="P5243_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRS19883.1"
                     /translation="MHIGFYEEDAKIVASFKKKQFRALVQHIVSKESSKTIDYINFIA
                     CTDSYLLAINKEYLKHDYFTDIITFDYSETEIASDIYVSIDRISENATSNSVSLLNEL
                     QRVLIHGVLHLVGYQDDTESNKQVMTQKENYYLHLLA"
     gene            566..>1416
                     /locus_tag="P5243_10290"
     CDS             566..>1416
                     /locus_tag="P5243_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS19884.1"
                     /translation="MLQSYDVIVVGAGHAGCEAAAAAARLGSQTLLITMDLTKMAAMS
                     CNPAVGGIAKGQIVREIDALGGCMGIITDMSSIQFRMLNKSKGPAMWSPRSQADKHVF
                     SQNWRKTLESISNLDLWQDSVVRLLFDSNRVIGVETALGISIYSKAVVLTNGTFLNGL
                     MHIGRSQLQGGRIGEPASYYLSDQLRDFGFTVDRMKTGTPVRIDGRTIDFSKLTEQPG
                     DEDIRNFSFLPHIQSSLPQLSCWITYTNSQVHEILHTGFDDSPMYNGTIQSIGPRYCP
                     SIETKLVT"
BASE COUNT          475 a          200 c          275 g          466 t
ORIGIN      
        1 tacccgatcc tacattgccc gaaaatatag aaaaagaaac cggaagaggt ttatttttaa
       61 tgaattgtct tgctgatgag gtagtatata atgataaagg aaacgaggta agtcttaaat
      121 ttttcataaa ttaatgcata taggctttta cgaagaagat gctaaaatag tagcatcatt
      181 taaaaaaaag caatttagag ctttagttca gcatattgtt tcaaaagaat cttctaaaac
      241 aattgactat attaacttta tagcttgtac tgattcttat ttattagcaa tcaacaaaga
      301 atatcttaaa cacgattatt ttaccgatat tataacattc gattattcag aaacagaaat
      361 agcatcagat atttatgtaa gtattgatag aatttctgaa aatgcaacgt ctaattctgt
      421 atctttgctc aatgaattac aacgagtttt aattcacgga gtattgcatt tggttggtta
      481 tcaagatgat acggaatcaa ataaacaggt aatgactcaa aaagaaaatt attatttgca
      541 tttattagca taagaaggtg cttgtatgtt acaatcgtat gatgttatag ttgttggagc
      601 cggacatgcc ggttgcgaag ctgctgctgc tgctgcacga ttaggttctc aaacgttgct
      661 tattaccatg gatttaacta aaatggctgc aatgtcgtgt aatcctgctg ttgggggtat
      721 agctaaaggt cagatagtga gagagataga tgcgttgggt ggctgtatgg ggattataac
      781 tgatatgtcg tctattcaat ttagaatgct aaacaaatct aagggacctg ctatgtggag
      841 tccacgttcg caagccgaca aacatgtttt ttctcaaaat tggcgtaaaa cattagaatc
      901 tatttctaat ttagatttat ggcaagattc tgttgttcgt ttgctatttg attcaaatag
      961 agttattggt gttgaaaccg cacttggtat ttcaatttat tctaaagcag tagttttaac
     1021 gaatggaact tttttaaatg gattaatgca cattggacgg tcgcaattac aaggaggtag
     1081 aataggagag cccgcatcat attatttatc agatcaactg cgtgattttg gttttactgt
     1141 tgatagaatg aaaacaggaa ctccggtaag aattgatgga agaacaattg attttagtaa
     1201 attaactgaa caacccggag atgaagatat aagaaatttt tcatttttac cacatataca
     1261 atcttcgtta cctcaattga gttgttggat tacatatacc aattctcagg tgcatgaaat
     1321 attgcataca ggctttgatg attcgccaat gtataatgga acaattcaaa gtataggtcc
     1381 gcgatattgt ccgagcatag aaactaaatt ggtaac
//
LOCUS       DAOTOW010000669         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_72792_length_1414_cov_4.125092, whole genome shotgun sequence.
ACCESSION   DAOTOW010000669 DAOTOW010000000
VERSION     DAOTOW010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72792_length_1414_cov_4.125092"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="P5243_10295"
     CDS             complement(<1..855)
                     /locus_tag="P5243_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19885.1"
                     /translation="MKKVFSILYSSIVLIFFSSNIYSQQLPTIADSLLFYFDMEGKIV
                     NKGIKKVKTELHSARQIVDKVGKAKSALEFYSQTGIYNRGLWIYEDINPINYPIFTIT
                     AWVQLANTSGSQKIMSLGNTAQTRGFGTTYEDGYTYWSANIGDSDPLLGSKVTGEWTF
                     IAVLYNQPAEEIRFVVNNTLYSSKGKLKKGVDVITIGNFEGKVDELRMYSRFLTPIEL
                     EQLYGAPMLFKDSDLLVKERVNYRAKQKQDDIAKLDSMPVRVVYSKELEVYDTIESKN
                     ILFYVKTND"
     gene            complement(1045..>1414)
                     /locus_tag="P5243_10300"
     CDS             complement(1045..>1414)
                     /locus_tag="P5243_10300"
                     /inference="COORDINATES: protein motif:HMM:NF039385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HRS19886.1"
                     /translation="KGMAEEKVYFENGDVKVTNARFLTFGKMHSMAGVTSVAKYVISP
                     SYKYPIITLVIGLILFAVSWIFALIVTGVAIAWIVSLKKEYQITLSSASGSEEALRSK
                     DEAFIDEIINALNQAIIDRG"
BASE COUNT          492 a          249 c          157 g          516 t
ORIGIN      
        1 atcatttgtt ttcacataaa atagaatatt tttagattca attgtatcat atacttctaa
       61 ttctttagaa tatactacac gaaccggcat actatctaat ttagctatat cgtcttgctt
      121 ttgttttgct cgataattaa ctcgttcttt aacaagaaga tcagaatctt taaaaagcat
      181 tggagcacca tataattgtt ctaactctat aggtgttaaa aaccttgaat acatacgcaa
      241 ctcatctact ttaccttcaa aattccctat tgttataaca tctacacctt ttttaagttt
      301 acctttacta gaatataacg tattgtttac aacaaatcta atttcttctg caggttgatt
      361 atataatacc gctataaacg tccattctcc ggtaacctta ctacctaata acggatcgct
      421 atcgccaata tttgcagacc aataagtata accatcttca taagtagttc caaatccgcg
      481 tgtttgagct gtattaccta aactcataat tttttgagaa ccgctggtat ttgcaagttg
      541 cacccatgct gtaatagtaa atattggata attaattgga ttaatatctt catatatcca
      601 taatcctcta ttgtatattc ctgtttgaga ataaaattct aatgctgact ttgctttacc
      661 aactttatca acaatttgtc ttgccgaatg aagttcagtt tttacttttt taattccttt
      721 atttacaatt ttcccttcca tgtcaaaata aaacaacaaa ctatcagcta tagtaggtag
      781 ctgttgagaa taaatatttg aagaaaagaa aattaaaaca atactactat aaagtataga
      841 aaatactttt ttcatttgat taccatttaa aaattatgtg gaaaaaaaat aatttacaag
      901 taatataaat tattacaaac ttaataaata atagtataat taaacattat accactatac
      961 atattaatgt acgatacaat tctatcgtat tttcttaaaa taggcgaatt caaaaaaaag
     1021 gctgtataaa cagccttaat attattatcc tctatcgata attgcttgat tcaaagcatt
     1081 tattatttca tcaataaatg cttcatcttt actacgaagt gcctcttcac ttccggatgc
     1141 tgaacttaaa gttatttgat attctttttt cagacttaca atccatgcta tagcaacacc
     1201 tgtaacaatt aatgcaaaaa tccaactcac tgcaaatagg attaatccga taactaatgt
     1261 aataattgga tatttataac ttggagatat tacatatttt gctacagatg taacacccgc
     1321 cattgaatgc attttaccaa atgttaagaa acgtgcgttt gtaactttca catcaccatt
     1381 ttcaaagtaa actttttctt ctgccatacc tttt
//
LOCUS       DAOTOW010000670         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_72818_length_1414_cov_2.767476, whole genome shotgun sequence.
ACCESSION   DAOTOW010000670 DAOTOW010000000
VERSION     DAOTOW010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72818_length_1414_cov_2.767476"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..>1414
                     /locus_tag="P5243_10305"
     CDS             231..>1414
                     /locus_tag="P5243_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRS19887.1"
                     /translation="MNFSILIIDNTSISQIIENIYDTIALNHTITELTGEQVLAEASI
                     QEPIHYIIIDSEDESYIMKLLNYFRSRRSFKHVPVLILSEHGITAFAEKFMAFGIVDF
                     LQKPVDEYLLTYSLLYTYHVSRSLYVLQNENLILKQKLINLEKLRVTNISLASASQRL
                     EDINFLLEESQSKIQNQQRELIEAKNKSEELLNNILPEEISRELMINGEAQPQFYKKV
                     TVMFTDFVGFTKVCEKREPQDMTQELNRHFSYFDDICEIHYLEKIKTIGDAYMCAGGI
                     PMRNNSNPFDIILAAMKMMAYMNIVNEEKMARNEPVWQIRIGIHTGSVVAGVIGKRKF
                     AYDIWGDAVNTAYRIESTSEAGKINISGDTYQLVKDFFECTYRGKIYAKNKGEIDMYF
                     VE"
BASE COUNT          523 a          188 c          248 g          455 t
ORIGIN      
        1 acttgccgaa acatcgggaa aattgaaagt agtagaaccc gataataaac ttataaaatc
       61 agctcgagct ttaggagtat gttttggtga cgaattatgt aaaataaaat aaagcatata
      121 gtgctacagt atacatttca ttatgtatat actaaaaaaa tatgtattta tatgtacaat
      181 tattctattt ttccctattt ttacataaat ttagaaacta atacgttcat atgaactttt
      241 caatactcat tattgataac acttcaatat ctcaaattat tgagaatatt tatgatacca
      301 tagccttaaa tcacacaatt acagagctta ccggtgagca ggttttggca gaagcaagta
      361 ttcaagaacc tatacattac ataataatag attccgaaga tgaatcgtat attatgaaat
      421 tattgaatta ttttcgttct cgaagatcat tcaaacatgt accggtatta atactttctg
      481 aacatggaat aactgcattt gccgaaaaat ttatggcatt tggtattgtt gattttttac
      541 aaaaacctgt tgatgagtat cttttaacat attcattgtt atacacgtat catgtaagta
      601 ggtcgttata tgttttgcaa aatgaaaatt taatactcaa gcaaaaatta ataaatcttg
      661 aaaaactgcg tgttacaaat atttctttgg cttcggcatc gcaacgttta gaagatatca
      721 attttttact cgaagaatct caaagtaaaa ttcaaaatca acaacgagaa ttgattgaag
      781 caaaaaataa atcagaagaa ttgttgaata atatattgcc cgaagaaatt tcgcgagaat
      841 taatgataaa tggcgaggct caaccgcaat tttataaaaa agtaacggta atgtttaccg
      901 actttgttgg gtttaccaaa gtgtgtgaaa aacgtgagcc acaagatatg acacaagaat
      961 taaatcgtca tttttcatat tttgatgata tctgtgaaat tcattatctc gaaaaaatta
     1021 aaacaatagg tgatgcgtat atgtgtgccg gaggtattcc tatgcgtaat aatagtaacc
     1081 cttttgatat aatattggct gccatgaaaa tgatggcata tatgaatatt gtaaatgaag
     1141 aaaaaatggc tcgcaatgaa ccggtgtggc aaattagaat tggaattcat accgggagtg
     1201 tggtagccgg agttattggt aaacgtaagt ttgcttatga tatttggggc gatgcggtaa
     1261 atactgcata ccgaattgaa agcaccagcg aggcaggtaa aataaatatt tcgggtgata
     1321 cgtatcaatt agtaaaagat ttttttgaat gtacataccg aggtaaaata tatgccaaaa
     1381 ataaaggtga aatagatatg tattttgtag aaag
//
LOCUS       DAOTOW010000671         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_73065_length_1411_cov_2.949115, whole genome shotgun sequence.
ACCESSION   DAOTOW010000671 DAOTOW010000000
VERSION     DAOTOW010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73065_length_1411_cov_2.949115"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="P5243_10310"
     CDS             <1..337
                     /locus_tag="P5243_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS19888.1"
                     /translation="AILGVAGGELIIPTLVLVYGVDIKLAGSLSLCISLPTMIVAFYR
                     YSQSGAITLFKRETSFIVSMAVGSIAGTAIGGQLIGYIPSNILVLLLGSILFISAIKT
                     FKKTKHSNN"
     gene            350..1093
                     /gene="modA"
                     /locus_tag="P5243_10315"
     CDS             350..1093
                     /gene="modA"
                     /locus_tag="P5243_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRS19889.1"
                     /translation="MKKLMMIACIALSISTQAQKVKVAAAADLRYAMDDIVKLYKQKN
                     PAADIEVMYGSSGHAYTQIANGAPFDIYFSADIMYPQKLKEAGLTISEPKLYAIGRIV
                     LWSSSVDVSKGIMVLNNMPHIKIATANPDHAPYGQRSIEALKYYKIYDKVQKQLIFGE
                     NISQAAQFCLTGNAEVGLLALSIVLSPAMSSKGSYYLIDNKTHRPLEQAYVLLKPAKD
                     NKAAYAFADFIATKPAREIFEKYGFTLPQ"
     gene            1110..>1411
                     /locus_tag="P5243_10320"
     CDS             1110..>1411
                     /locus_tag="P5243_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19890.1"
                     /translation="MNTLPGIITHIQQSGAILLVDVQVENIQISALLIESTYTPDWLY
                     KNSPVFVVCKETEISIAKNLSGSISTRNRIPCNVTNVERGELLSTIYLTYATYHLQA"
BASE COUNT          481 a          242 c          254 g          434 t
ORIGIN      
        1 agctattctt ggagtagcag gtggtgaact cattattcca acattggtac ttgtatatgg
       61 cgttgacata aaacttgccg gaagtctttc gctctgtatt agcttaccta ccatgattgt
      121 ggcattttac aggtattctc aaagcggagc aataactctg tttaaacgcg aaacctcatt
      181 tattgtatct atggctgttg gatctattgc aggcactgct attggaggtc aattaatagg
      241 atatattcct tctaatatcc ttgtactgct tttgggatca atattattta tttctgcaat
      301 taaaacattt aaaaaaacaa aacatagtaa caattaacat tcaaatgata tgaaaaaact
      361 aatgatgatt gcttgcatag ctttaagtat aagcacacaa gcgcaaaaag taaaggtagc
      421 ggcggcggcc gatttacgat acgctatgga tgatatagta aagttgtata aacagaaaaa
      481 tcccgcagca gatatagaag ttatgtatgg ttcatcaggt catgcatata cacaaattgc
      541 taacggagct ccctttgata tttatttttc ggctgatatt atgtatccgc aaaaattaaa
      601 agaagccgga ttaactataa gcgaacctaa actgtatgct attggtagaa ttgttttgtg
      661 gagttcgagt gtagatgttt caaaaggaat aatggtactc aacaatatgc ctcacattaa
      721 aattgcaacg gcaaaccccg accatgctcc gtatggacaa cgttctattg aagcattaaa
      781 atattacaaa atatatgata aggtacaaaa acaattaatt tttggtgaaa atatatcgca
      841 ggctgctcag ttttgcctta ccggtaatgc agaagttggc ttgttggcat tgtctattgt
      901 gttatcacct gctatgtctt caaaagggtc atattatctt attgataata aaacgcatcg
      961 ccctttggaa caagcatacg tattattgaa acctgcaaaa gacaataaag cggcatatgc
     1021 ctttgctgat tttatagcaa caaaaccggc acgtgaaatt tttgaaaaat acgggtttac
     1081 cttacctcaa taattaaaca attagcatta tgaatacttt accgggcatc attacacaca
     1141 ttcaacaatc gggagctata ctcttggttg atgtacaagt agaaaatatt caaatatctg
     1201 cactcttgat agaatctaca tatactcccg attggttata caaaaattct ccagtatttg
     1261 tagtatgtaa agaaacagaa ataagtattg caaaaaattt gagtggaagc ataagcacac
     1321 gtaatagaat accatgtaac gtaaccaacg ttgaacgagg agaattactc agcactattt
     1381 atttaacgta tgctacttat catttgcaag c
//
LOCUS       DAOTOW010000672         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_73282_length_1408_cov_3.933481, whole genome shotgun sequence.
ACCESSION   DAOTOW010000672 DAOTOW010000000
VERSION     DAOTOW010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73282_length_1408_cov_3.933481"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1408)
                     /gene="thrS"
                     /locus_tag="P5243_10325"
     CDS             complement(<1..>1408)
                     /gene="thrS"
                     /locus_tag="P5243_10325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRS19891.1"
                     /translation="IKFGIGPAIENGFYYDVDPGTAIITEKDLAKIEETMLELARQKN
                     EYIRKNVSKAEALEYFSKKNEEYKVELIQELEDGTITYYTQGSFTDLCRGPHLPNTDH
                     IKAVKLLNIAGAYWRGDEKRKQLTRIYGITFPKKKMLDEYLELLEQAKQRDHRKVGKE
                     LELFMFSNRVGQGLPMWLPKGAALREKLEQFLKNVQRKHGYSQVITPHIGQKELYVTS
                     GHYAKYGKDSFQPIHTPAEGEEFLLKPMNCPHHCEIYKNKPRSYKDLPLRLAEFGTVY
                     RYEQSGELHGLTRVRGFTQDDAHIFCTPEQLKEEFCKVIDIIFHIFKALDFKEFEAQV
                     SLRDKNNTEKYIGSDENWEKAERAIIEAAAEKGLQTIVEYGEAAFYGPKLDFMIKDAI
                     GRRWQLGTIQVDYNLPERFELEYIGADDQRYRPVMIHRAPFGSMERFVAVLIEHTGGK
                     FPLWLTPEQVVVIPVSEK"
BASE COUNT          361 a          271 c          249 g          527 t
ORIGIN      
        1 tatttttcgc tcacaggtat tactacaact tgttccggag tcaaccataa cgggaattta
       61 ccaccggtat gttcaattaa aacagcaaca aatcgttcca tagaaccaaa cggagcacgg
      121 tgaatcatta ccggtctgta tcgttggtcg tctgctccaa tatactctaa ttcaaaacgt
      181 tctggtaggt tatagtcaac ttgtatagtg ccaagttgcc aacggcgtcc tatagcgtct
      241 tttatcataa agtcgagttt gggaccatag aatgcagctt caccatattc tacaattgtt
      301 tgcagacctt tttctgctgc agcttctata attgcacgtt ctgctttttc ccaattttcg
      361 tcgctgccta tatatttttc tgtgttgttt ttgtcgcgta acgatacttg ggcttcaaat
      421 tctttaaaat ccagtgcttt aaatatatgg aatataatat caattacttt acagaattct
      481 tctttaagtt gttccggggt acaaaatata tgggcgtcgt cttgagtaaa tcctcttaca
      541 cgtgtaagac catgcaattc tccgctttgt tcgtaacgat atacggttcc aaattcagct
      601 aaacgtagcg gtaaatcttt gtatgaacgt ggtttgtttt tgtatatctc gcagtgatga
      661 gggcagttca ttggtttcaa caagaattct tctccttcgg caggtgtatg aattggttga
      721 aacgaatctt taccatattt tgcgtagtgt ccagatgtta catataattc tttttgccct
      781 atatgtggtg taattacctg gctgtaacca tgtttgcgtt gtacgttttt gagaaattgt
      841 tctaattttt ctcgtaatgc agctccttta ggcaaccaca ttgggagtcc ttgaccaact
      901 cggtttgaga acataaacaa ttccaactct ttaccaactt tacggtggtc gcgttgtttt
      961 gcttgttcaa gtaattcaag atattcatca agcattttct tttttggaaa agtaatgcca
     1021 tatatgcgag taagttgttt acgtttttca tcgcctcgcc aatatgctcc tgctatattt
     1081 aataatttta cggcttttat atggtcggtg tttggaagat gaggaccacg acacaaatcg
     1141 gtgaatgaac cttgtgtata gtaagtaatg gtgccatctt ctaattcctg tattaattca
     1201 actttatatt cttcattttt ttttgaaaaa tattctaacg cttctgcttt acttacattt
     1261 ttacgaatgt actcgttttt ttgacgagca agttcaagca ttgtttcttc aatttttgct
     1321 aaatcttttt ctgttattat agctgttccc ggatctacat catagtaaaa tccattttct
     1381 attgcagggc ctattccaaa tttaattc
//
LOCUS       DAOTOW010000673         1401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_73873_length_1401_cov_2.811293, whole genome shotgun sequence.
ACCESSION   DAOTOW010000673 DAOTOW010000000
VERSION     DAOTOW010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73873_length_1401_cov_2.811293"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1401)
                     /locus_tag="P5243_10330"
     CDS             complement(<1..>1401)
                     /locus_tag="P5243_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS19892.1"
                     /translation="YYTVTLTDACSSITDSIDIEEPLELELELTSTNITCIGALNGSA
                     LALVNGGTVPYSYIWSNGKNTQGISSLPQGKYTVQVQDSRGCKKMLTTTITEPEPLQA
                     QITATNATCFGKSNGSILVQVSGGTLPYSYNWNNSFTDANNLNLPAGTYSVTITDACD
                     DNIVKTATIQEPQKLTYTMLVEDVTCFGKSDGTIRIEASKGVEPYTISWNNQFQGFTQ
                     KNIPAGTYECIISDACNDSYTIQSTVNQPDLFDIDVESNNVTCNGLGNGSATIIPKGG
                     VEPYLYRWNIGLQTKTVQGLFPGLYSVNVYDARGCLASEVFTISQPETLDIQLQVTNA
                     ECNAQNGAISASVIGGTAPYSYNWSQQSQTQNIEGLAQGKYTLTVTDRNNCTRTAQAT
                     VSIETPSYPICLVTIDRDLGTNKIIWEKVWNSSIEKVHIYKMSGGEYKWIGSVDSVRQ
                     SYFDDYITNPLQSPSR"
BASE COUNT          399 a          249 c          250 g          503 t
ORIGIN      
        1 atcgtgatgg agattgtaat ggatttgtaa tataatcatc aaaatatgat tgtcgtacag
       61 aatctacact gcctatccat ttatattcac ctccactcat tttatatata tgaacttttt
      121 ctatacttga attccaaact ttttcccata ttattttgtt ggtacctaaa tctctgtcaa
      181 ttgtaactaa acaaattggg taggaaggag tttctatact tacggttgct tgtgctgttc
      241 gtgtacagtt atttctatca gttacggtta gagtatattt gccttgagcc aatccttcta
      301 tattttgtgt ttgcgattgt tgtgaccaat tgtatgaata tggtgctgtt ccgccaataa
      361 cagaagcaga tatagctccg ttttgagcat tacattctgc gtttgtcact tgtaattgaa
      421 tatctaatgt ctcgggttgc gaaatagtaa atacttcaga agctaagcaa cctcgagcat
      481 catacacatt aacactgtat aacccaggaa ataatccttg cactgttttg gtttgtaatc
      541 ctatattcca acggtacaaa tatggctcta ctccgccttt gggaataata gttgccgaac
      601 cattacctaa tccattgcac gtaacattgt ttgattctac atctatatca aataaatcgg
      661 gttggttaac cgtactttgt attgtatatg agtcgttaca agcatcgctt ataatacatt
      721 catatgttcc tgcaggaata tttttttgag taaatccttg aaattggtta ttccatgaaa
      781 ttgtatatgg ttcaacacct ttacttgctt ctatacgtat agttccatct gatttaccga
      841 agcatgtaac atcttcaacc aacattgtat aggttaattt ttgaggttct tgtatagttg
      901 ctgtttttac aatattgtcg tcgcaggcat cggtaattgt tacagaatat gttccagccg
      961 gtagatttaa attattggca tcggtaaaag aattattcca attgtatgaa tacggtaaag
     1021 ttcctccgct cacctgaacg agaattgaac catttgattt gccaaaacat gttgcattgg
     1081 tggcggtaat ttgagcttgc aaaggttcgg gttcagtaat agtagtagta agcatttttt
     1141 tacaaccgcg agaatcttgt acttgaacag tatatttgcc ttgtggtaaa ctcgaaattc
     1201 cttgtgtgtt tttgccatta ctccaaatgt atgagtatgg tacagttcct ccatttacca
     1261 atgccaatgc acttccattg agagctccta tacaggtaat attggttgaa gttagttcta
     1321 attctaattc aagaggctct tctatatcaa tgctatcagt aatagaagag caagcatctg
     1381 taagagtaac agtatagtag c
//
LOCUS       DAOTOW010000674         1400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_73971_length_1400_cov_3.138290, whole genome shotgun sequence.
ACCESSION   DAOTOW010000674 DAOTOW010000000
VERSION     DAOTOW010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73971_length_1400_cov_3.138290"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="P5243_10335"
     CDS             <1..554
                     /locus_tag="P5243_10335"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRS19893.1"
                     /translation="NAHDIVEKLGLPCFVKPNNGGSSFGVSKVKSVEDIIPAIHKALL
                     EDSEVIIESFIQGTEISNGVYKIGNNITVLPITEIRTDNEFFDYQAKYEGKSKEITPA
                     EISEELTNKCKKLTAQIFSHLDCKGICRVDYIIQNNELYMLEINTVPGMSKASIIPQQ
                     IRAAGMKESDVFTAIINDVLHTK"
     gene            complement(643..>1400)
                     /locus_tag="P5243_10340"
     CDS             complement(643..>1400)
                     /locus_tag="P5243_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS19894.1"
                     /translation="TAVTTFLNSVNGINTSTLQDKIDQATSTLLLVTNNTGNQPGNYP
                     FSSVTALNNAITVAQNVLNTATTQVQITDAVNTLQNEITSFLNSVVPYPVNVATLQDL
                     IDIAEAKIATAQIGNQIGQYPANTFNALYAELITANTLMLNPNATQTDIDAQVVTLQN
                     VYNAFLASVRTNIEVVEQTNCIVNANNQMVMLESHEVIKQVVITTVLGTTTTLLVNEK
                     SAQVSVESIAKGVYFVTVVFANGTQQTIRLVKK"
BASE COUNT          504 a          204 c          217 g          475 t
ORIGIN      
        1 ttaatgctca tgacattgtt gaaaaattag gcttaccatg ttttgtaaaa ccaaataacg
       61 gaggctcaag ttttggtgtt tctaaagtta aatcagttga agatataata cctgcaatac
      121 acaaagcatt actcgaagat tctgaagtta ttattgaaag ttttattcaa ggtacagaaa
      181 taagcaatgg tgtatataaa attggaaata atataaccgt attgccaatt accgaaatca
      241 gaacagataa tgaatttttt gactatcagg caaaatatga aggaaaaagt aaagaaatta
      301 cacctgcaga aatttcagaa gaattaacaa acaaatgcaa aaaactaaca gctcaaatat
      361 tttcacacct tgattgtaaa ggaatatgta gggttgatta tatcatacaa aacaatgaat
      421 tgtatatgct cgaaataaat actgttccgg gaatgagtaa agcaagtata attcctcaac
      481 aaataagagc tgcaggcatg aaagaatccg acgtttttac agctattata aatgatgtgc
      541 ttcacacaaa ataattgtat gtaaaataca aatgaataaa aaaggtggat atattcatac
      601 tatccacctt tttgtttatg ccattactaa aataaaccta ttttattttt tgactaatcg
      661 tattgtttgt tgtgttccat tagcaaaaac tactgttaca aaataaacac cttttgctat
      721 agactctaca gaaacttgag ctgatttttc attcacaagt aacgtagttg tagttcctaa
      781 tacagtagtt ataacaactt gtttaattac ttcatgagat tctaacatta ccatttgatt
      841 atttgcattc acaatacaat tagtttgttc tacaacttca atattggtac gtaccgatgc
      901 cagaaaagca ttatacacat tttgtaatgt aacaacctga gcatcaatat cagtttgtgt
      961 tgcatttgga ttcaacatca acgtattagc agtaattaat tctgcatata atgcattaaa
     1021 agtatttgca ggatattgtc ctatttgatt tccaatttgt gcagtagcta ttttagcttc
     1081 agcaatatca attaaatctt gtaaagttgc aacatttaca ggatatggta caactgaatt
     1141 taaaaatgat gtaatttcat tttgcaaggt atttacagca tctgtaattt gtacttgcgt
     1201 agttgctgta ttcaacacat tttgagctac agtaatagca ttgtttaatg cagtaaccga
     1261 tgagaacgga taattacccg gttggtttcc ggtattatta gttaccaata ataatgttga
     1321 agtagcttga tctattttat cttgtaatgt tgaagtatta attccattta ccgaattcaa
     1381 gaaagtagta actgctgtat
//
LOCUS       DAOTOW010000675         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_74050_length_1399_cov_3.315476, whole genome shotgun sequence.
ACCESSION   DAOTOW010000675 DAOTOW010000000
VERSION     DAOTOW010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74050_length_1399_cov_3.315476"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..>1399)
                     /locus_tag="P5243_10345"
     CDS             complement(43..>1399)
                     /locus_tag="P5243_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HRS19895.1"
                     /translation="KNNIPLTSNQAYGLQEGVEYTITVSDALGSSTTKKFKIPPSSAT
                     EKINHFFGSMVDAIASVLMVDIFALFKLYDPVIYDSQGKAILHPNGTPKRTNIPLIVI
                     WLFLGALYFTIRFKFINIRGFIHSIQLVRGKYDTPDDKGQLSHFQALATALSATVGLG
                     NIAGVALAIGIGGPGATFWMILMGILGMSSKFTEAALGVKYRRINNKGEVSGGPMYYL
                     QYVFGTKTYIRKKMGKLLALFFAILLIGSSLGGGNMFQANQSFANVAQMIPSLQNHAI
                     LFGLGLAFLVGIVILGGITSIAKVTSKIVPIMAGIYLVTALIIIFTNFSHIGIVAKAI
                     YNGAFHADSIYGGFIGVLVMGIQRAAFSNEAGIGSAAIAHSTVKTQAPVSEGFVALLE
                     PFIDTVVICSLTAFVLIFTGYYTPEYTSQFQGANLTSEAFGSVFPWFKWVLLITILLF
                     V"
BASE COUNT          458 a          252 c          239 g          450 t
ORIGIN      
        1 aaaggaaggg ggagacgttt ttccattaac aacgtggtga aactaaacaa atagtaatat
       61 tgttattaaa agaacccatt taaaccatgg gaaaacgctg ccaaatgctt ctgatgttaa
      121 attggctcct tggaattgcg atgtgtactc aggagtatag taaccggtaa aaataagaac
      181 aaatgccgta agcgagcata ttacaacagt gtcaataaat ggttcgagta atgcaacaaa
      241 tccttcgcta actggagctt gtgtttttac cgtagagtga gcaattgccg cagaacctat
      301 accggcttcg ttactaaagg ctgctcgttg tattcccatt actaaaacgc ctataaatcc
      361 tccatagatt gaatcggcat gaaatgcacc attgtatata gcttttgcaa ctattccaat
      421 gtgcgagaag ttagtaaata ttataattag tgcagtcact aaataaattc ctgccataat
      481 tggaactatt ttacttgtta ctttggctat actggtaata cctcctaata tcacaatacc
      541 aactaaaaat gctaatccta gaccaaataa tatagcgtga ttttgtaatg aaggaatcat
      601 ttgtgctaca tttgcaaatg attgatttgc ctgaaacatg ttaccaccac ctaatgaact
      661 gcctataagc agtattgcaa aaaatagtgc gagtagtttc cccattttct tacgaatata
      721 ggttttggtt ccaaaaacat attgtaaata atacatcgga ccacctgata cttctccttt
      781 gttattgatt cttctatatt ttacaccaag agctgcttct gtaaattttg atgacatacc
      841 taatattccc atgagaatca tccaaaaagt tgctccgggg cctcctatac caattgctaa
      901 tgctacacct gcaatattac ctaatccaac agttgcagaa agagctgttg ctaatgcttg
      961 aaagtgagat aattgtcctt tatcgtcagg tgtatcgtat ttaccccgta ccaattgaat
     1021 agagtgtata aatcctcgta tatttataaa tttaaaacga atagtaaaat agagtgctcc
     1081 taaaaataac caaattacaa tgagcggtat attggttctt tttggagttc cgttcggatg
     1141 taaaattgct tttccttgag aatcataaat aaccgggtcg tataatttga ataaagcaaa
     1201 aatatcaacc ataagaacag aggctattgc atctaccata ctaccaaaaa aatgatttat
     1261 tttttctgtt gcagatgaag gtggaatttt aaattttttt gttgtggacg aacctaatgc
     1321 atcacttacg gtaattgtgt attctactcc ttcttgtaac ccatatgctt gattcgaagt
     1381 taatggtata ttgtttttg
//
LOCUS       DAOTOW010000676         1394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_74489_length_1394_cov_2.214339, whole genome shotgun sequence.
ACCESSION   DAOTOW010000676 DAOTOW010000000
VERSION     DAOTOW010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74489_length_1394_cov_2.214339"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5243_10350"
     CDS             <1..893
                     /locus_tag="P5243_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRS19896.1"
                     /translation="EKGGEIIPKIVSVDVQTRHLFAQKIQFITHCPECGTELIRLQGE
                     SAYYCPNQYNCSPQIRGRIEHFVSRKAMDIGLAEATIEQLFTQGLIHDAGDLYSLTYE
                     QLIQLDRFAEKSVRNLLQSIEQSKQVPYYRVLYALGIRYVGETVAKTLAKAIPSIEML
                     QAASIMQLEQIPEIGTKIAESIKQHFREPYSLNIIEKLKQAGVQLSTVDTEHKQVNNV
                     LQGASVIVSGVFQSYSRDEMKELIEQYGGRNVTSISKQTSCMIAGDGVGPSKLQKAQE
                     LGIPILTEDEFLVKIGLKSV"
     gene            1027..>1394
                     /locus_tag="P5243_10355"
     CDS             1027..>1394
                     /locus_tag="P5243_10355"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRS19897.1"
                     /translation="MEALYVFIATVFSYLLGSIPTSVWIGKAFYGIDIREHGSGNAGA
                     TNTFRVLGKKAGIPVFAFDVFKSFFASKLVIFFPYLIEGTPHYVNLQLLFGISAVIGH
                     IFPIYVGFKGGKGVASLLGLA"
BASE COUNT          491 a          179 c          263 g          461 t
ORIGIN      
        1 ttgaaaaagg aggtgagata attccaaaaa ttgttagtgt tgatgtacaa acacgacatt
       61 tgtttgctca aaaaatacaa tttattactc attgtcccga atgtggtaca gaacttattc
      121 gcttgcaagg tgaatccgca tattattgtc caaatcaata caattgttca cctcaaatac
      181 gaggacgaat agaacatttt gtaagtcgta aagcaatgga tataggttta gcagaagcaa
      241 ctatagaaca attgtttacg caaggattaa ttcatgatgc aggcgattta tattcactta
      301 catatgaaca attaattcaa ttagacagat ttgccgaaaa atcggtgcga aatcttttgc
      361 aaagtataga acaatcaaaa caagttccat attatagagt attgtatgct ttaggaattc
      421 gatatgtagg cgaaacagta gctaaaacat tggctaaagc aatcccgtct atagaaatgt
      481 tgcaggcagc aagtattatg caattggagc aaatacctga aataggtact aaaatagctg
      541 aaagtataaa acaacatttt agagaaccat attctttgaa tattatagaa aagttgaaac
      601 aagcaggggt tcaattaagt actgttgata cagaacataa acaagtaaac aatgtattac
      661 aaggtgcatc tgtgattgtg tcgggagtgt ttcaatcata ttcgcgtgat gaaatgaaag
      721 agctaattga acaatatgga ggcagaaatg tgacatctat ttcaaaacaa acttcatgta
      781 tgatagctgg tgatggagta ggtccttcaa aattgcaaaa agctcaagaa ttggggattc
      841 caattcttac tgaagacgaa tttttagtga aaattggcct aaaatctgta taattatatg
      901 aatttgtagt ttttttaagt atttgatatt gttatgatta tgtattctaa ttgttaaaaa
      961 aacattaaaa gatttggcta taccaaaaaa atatatgatt tttgtataaa attagagtga
     1021 ccccttatgg aagcattata cgtttttata gcaacagtat tttcttactt attaggttct
     1081 atacctacat cggtatggat tggtaaagca ttttatggga ttgatataag agaacatgga
     1141 agtggaaatg ccggtgctac aaatactttt agagtattag gtaaaaaagc aggaatacct
     1201 gtgtttgcgt ttgatgtatt taaatcattt tttgcatcta aattagtaat attttttcca
     1261 tatttaatag aaggaactcc tcattatgtg aatttgcaat tgttatttgg tatttcggca
     1321 gtcattggtc atatttttcc tatttatgtt ggattcaaag gaggtaaagg tgttgcttca
     1381 ttgttaggac ttgc
//
LOCUS       DAOTOW010000677         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_74886_length_1389_cov_2.694153, whole genome shotgun sequence.
ACCESSION   DAOTOW010000677 DAOTOW010000000
VERSION     DAOTOW010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74886_length_1389_cov_2.694153"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="P5243_10360"
     CDS             <1..908
                     /locus_tag="P5243_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRS19898.1"
                     /translation="NSIIQYLSLENFGAFFLSNINMKRIAIQGIQGAFHEIAAIQYFG
                     NNIETVNCEKFKQIFELVSNGSVHYGVVAIENTIAGSLLGNYNLLKESKVKIIGELSL
                     HITQNLMALPGTTIETIREIYSHPIAIQQSMEFLSNYPHIKLIEWSDTASSAQKIATE
                     QLHNTGAIASKYAAELYGLQILASEIETHKMNYTRFLIIQRNSHDVPENNKASLCFEL
                     GHQPGSLVDVLTVFKNEEINLTKIQSLPILGRPYQYSFYIDLEWKDRARYDIALNKAL
                     KIVSNLSVLGEYVRDEKFNHEMIQI"
     gene            914..>1389
                     /locus_tag="P5243_10365"
     CDS             914..>1389
                     /locus_tag="P5243_10365"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRS19899.1"
                     /translation="MNIVIIGVGLIGGSVALAIRGFNTHIIGVDSNADHANKALELGI
                     IDEISDLDNALHKAQLIILAIPVDAARKLLPYILDNIQDNTVVIDMGSTKVGICNNVR
                     SHARRSQYVASHPMAGTENSGPEAAFADLFKGKKTIICEKELSADFALQQAELLYK"
BASE COUNT          521 a          231 c          236 g          401 t
ORIGIN      
        1 aaaacagcat aatacaatat ctaagcctcg aaaatttcgg ggcttttttt ttaagtaata
       61 taaatatgaa acgaatagca atacaaggaa ttcaaggtgc atttcacgaa atagcagcaa
      121 tacaatattt tggcaacaat atagaaactg taaactgtga aaaatttaaa caaatttttg
      181 aattagtttc aaacggttcg gtacattatg gcgttgttgc aatagaaaat acaatagcgg
      241 gtagtttgtt gggcaattac aatttactca aagaatctaa agtaaaaata ataggcgaac
      301 tgagtttaca tataactcaa aaccttatgg cattgccggg aaccactatt gaaactattc
      361 gtgaaattta ctcgcatcct attgcaatac aacagtctat ggagttttta tcaaattatc
      421 ctcatataaa actcatagaa tggagcgata cagcttcgag tgcccaaaaa attgcaaccg
      481 aacaattaca caatacagga gcaattgcaa gtaaatatgc tgcagaatta tacggattac
      541 aaattttggc aagtgaaata gaaacacaca aaatgaacta tacccgtttt ttaataattc
      601 aacgcaattc acatgatgtg cctgaaaata ataaagcaag cttatgtttt gaattagggc
      661 atcaaccggg gtcattagtt gatgttctta ccgttttcaa aaatgaagaa ataaacctta
      721 caaaaattca atcattacca attttaggcc gtccgtatca atattctttt tatatagatt
      781 tagaatggaa agacagagcc agatacgaca ttgctctcaa taaagctctt aaaattgtat
      841 cgaatttatc ggtacttggc gaatatgttc gcgatgaaaa atttaatcat gaaatgatac
      901 aaatataaat agtatgaata ttgtaataat aggtgtagga cttataggag gctctgttgc
      961 attagcaata cgaggtttca acactcatat aataggtgta gattctaatg ccgaccatgc
     1021 aaacaaagca ttagaattag gaatcattga cgagattagt gatcttgaca atgcattaca
     1081 caaagctcaa ctcataattc ttgcaattcc ggtagatgcc gcacgaaaat tattgccgta
     1141 tatacttgac aacattcaag ataatactgt agtaatagat atgggctcta ccaaagtagg
     1201 aatttgcaac aatgttcgtt ctcatgctcg acgttcacaa tatgtagcat cgcacccaat
     1261 ggcaggaacc gaaaactcgg gtcctgaagc tgcttttgcc gatttattta aaggaaaaaa
     1321 aacaatcatt tgcgaaaaag aattaagtgc agactttgca ttacaacaag ctgaattact
     1381 ctacaaaat
//
LOCUS       DAOTOW010000678         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_75039_length_1387_cov_3.774024, whole genome shotgun sequence.
ACCESSION   DAOTOW010000678 DAOTOW010000000
VERSION     DAOTOW010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75039_length_1387_cov_3.774024"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="P5243_10370"
     CDS             <1..1018
                     /locus_tag="P5243_10370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS19900.1"
                     /translation="AAYNEEQVIEEKIKSIFNTSYPHHLFDVHIGSDNSSDATNSIIQ
                     TYCAQYSNLHLHAFTTRQGKAQIINTLTEQAKGSIIVLTDANVYFEYTTLYELVKHYK
                     NEDIALVGGLIINTNVKKTGISYQEKTYLTFENTIKYREGVIWGTMIGAFGGVYSIRK
                     EYYHPVPKNYFMDDFYITMHVLQDGKKAILEPKAVCFEDISNIIYEEFRRKVRISIGN
                     FQNLQTFKSLAYNIFTGLGFSFVSHKILRWLTPFFLIICFVLSGVLAIDSILYLYVFT
                     IQLLLMFIPLIDFLLQKFSIHIKVIRFITHFYSMNVALFLGFFKYLKGINSNVWQPTK
                     RNQN"
     gene            984..>1387
                     /locus_tag="P5243_10375"
     CDS             984..>1387
                     /locus_tag="P5243_10375"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRS19901.1"
                     /translation="MCGNQQNETKIKLHSIEEAIEDIRNGKIVIVVDNEDRENEGDFI
                     CAAELVTPEIINFMATHGRGLICASLPEYRCDELDLKLMVGRNTATFETPFTVSVDLI
                     GHGCTTGISVHDRAKTIKALVDPNTKPEDLGRP"
BASE COUNT          504 a          176 c          238 g          469 t
ORIGIN      
        1 agcagcatac aatgaagaac aggttataga agaaaaaatc aaaagcattt ttaacacttc
       61 ttatccacat catttatttg atgtgcatat aggttctgat aattcaagcg atgctacaaa
      121 tagtattatt caaacatatt gtgctcaata ttcaaatttg catttacatg cttttactac
      181 acgccaaggt aaagcgcaaa ttattaatac tttaacagag caagctaagg ggagtattat
      241 agtattaacc gatgctaacg tgtattttga atatactact ctatatgaat tggtaaaaca
      301 ctataaaaat gaagatatag ctttggtggg agggttaata attaatacaa atgttaaaaa
      361 aacaggaatt tcgtatcaag aaaaaacata tttaacattt gaaaatacta ttaaatacag
      421 agaaggagtt atttggggaa ctatgattgg tgcattcgga ggagtatatt ctattcgtaa
      481 agaatattat catccggttc ctaaaaatta ttttatggat gatttttata taacaatgca
      541 tgtgttacaa gatggtaaaa aagccattct tgaacctaaa gctgtttgtt ttgaagatat
      601 ttctaatata atatatgaag aatttagacg aaaagtaaga atatcaattg gtaatttcca
      661 aaatttacaa acatttaaat cattagcata caatatattt accggattag gatttagctt
      721 tgtatcacat aaaatattac gatggcttac cccattcttc cttattatat gttttgtgtt
      781 gtcaggagta ttagctattg atagtatatt gtatttgtat gtttttacaa tacaattatt
      841 gttaatgttt attccattga tagatttttt gttgcaaaag ttttcaattc acataaaagt
      901 aatacggttt ataacccatt tttattcaat gaacgtggcg ttatttttag gtttttttaa
      961 atatttaaaa ggaataaatt caaatgtgtg gcaaccaaca aaacgaaacc aaaattaagc
     1021 ttcattcaat agaagaagct atagaagata ttcgcaacgg gaaaatagta atagttgtgg
     1081 ataatgagga tagagaaaac gaaggcgatt ttatttgtgc tgcagaactt gtaactcccg
     1141 aaattattaa tttcatggca actcatggta ggggattaat atgtgcatca ttacccgaat
     1201 ataggtgcga tgaattggat ttaaaactta tggttgggcg taatacagct acttttgaga
     1261 caccttttac agtttcagtt gatttaatag gtcatgggtg tactacaggt atttcggtac
     1321 atgatagagc aaaaactata aaagcattag ttgaccctaa tacaaaacca gaagatttgg
     1381 gtaggcc
//
LOCUS       DAOTOW010000679         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_75512_length_1382_cov_2.228335, whole genome shotgun sequence.
ACCESSION   DAOTOW010000679 DAOTOW010000000
VERSION     DAOTOW010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75512_length_1382_cov_2.228335"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="P5243_10380"
     CDS             <1..384
                     /locus_tag="P5243_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRS19902.1"
                     /translation="KSIEKDIVLTYTSFGIHRDDITFVFNNSEIKQVGSQGQKKSFLL
                     ALKFAQYQLLYTKTGVLPILLLDDLFDKLDKERTAHVFDVIEGEQFGQIFITDTDKAL
                     LHTFFEARKQTGHFHELKDGKLIRL"
     gene            452..1024
                     /locus_tag="P5243_10385"
     CDS             452..1024
                     /locus_tag="P5243_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRS19903.1"
                     /translation="MQALLESEFIESQRILQDFIQQKHIFATLESVVHCMLKSIQQGN
                     TIITCGNGGSMTDAMHLAEELSGKFRKPRKALPAVAISDPSFMSCVANDYGYEYVFAR
                     FIESMGKPGDVLVAFTTSGNSKNIVLALQQAKQQNMKTIVLTGNTGGQAKHHADYTIC
                     VPHVGYADRIQEIHIKIIHTLIHAIEQNLL"
     gene            1041..>1382
                     /locus_tag="P5243_10390"
     CDS             1041..>1382
                     /locus_tag="P5243_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRS19904.1"
                     /translation="MLVKAFGGAVNGIDARIITVETDVVAGANFFIVGLPDSAVKESQ
                     QRIESALRSSGYKLPGKKITINMAPADIRKEGTSYDLTLAIGILAASEQMKCAEVDKY
                     LIMGELSLDGGV"
BASE COUNT          477 a          207 c          267 g          431 t
ORIGIN      
        1 aaaagtattg aaaaagatat tgtgcttaca tatacatcgt ttggcataca tcgtgacgat
       61 attacttttg tttttaataa ttcagaaatt aaacaagtag gatcacaagg tcaaaaaaaa
      121 tcatttttat tggctcttaa atttgcacag tatcaattac tctatacaaa aacaggagtg
      181 ttacctatat tattactcga tgatttattt gataaacttg ataaagaaag aacagctcat
      241 gtgtttgatg taatagaagg tgaacaattt ggacagattt ttataacaga tacagataaa
      301 gccctgttac atactttttt tgaagctcgt aagcagaccg gacattttca tgaactaaaa
      361 gatggaaagc ttatacggtt atagaaaaaa gtgtttatga cactatgaga acatattttt
      421 tttattattt ttgtcaaaac aaaacatgta tatgcaagca ctcttagagt ccgaatttat
      481 tgaatcacaa cgtatattgc aagattttat tcagcaaaag cacatttttg ctacattaga
      541 atctgttgta cattgtatgt tgaagtcaat tcaacagggg aatacaataa ttacgtgtgg
      601 caatggtggc tctatgactg atgctatgca tttagccgaa gaattatcgg gaaaatttag
      661 aaaaccgcga aaagcgttgc ctgctgtagc tattagtgac ccatcgttta tgtcgtgtgt
      721 ggctaatgat tatggatatg aatatgtatt tgcacgtttt atagaatcta tgggtaaacc
      781 aggagatgta ttagtagcgt ttacaacatc aggtaattca aaaaatatag ttcttgcttt
      841 gcaacaggca aaacaacaaa atatgaaaac aattgtactt accggaaata ccggtggtca
      901 agcaaaacac catgccgatt atactatatg tgtaccacat gtaggatatg ccgatagaat
      961 tcaagaaata cacataaaaa tcattcatac acttattcat gccattgagc agaatttgtt
     1021 gtagaaaaaa ctgacatact atgcttgtaa aagcttttgg cggggctgtc aatggaattg
     1081 atgcccgcat aattactgtg gaaaccgatg tggttgccgg agcaaacttt tttattgtag
     1141 gtttgcccga tagtgctgta aaagaaagtc aacaacgaat agaatctgcg ttgagaagtt
     1201 ccggttataa attacccggt aaaaaaatta ctataaatat ggcacctgcc gatattcgaa
     1261 aagaaggcac gtcgtatgat ttaactttgg ctattggcat tcttgctgcg tcagaacaaa
     1321 tgaaatgcgc agaagttgat aaatatctta ttatgggaga attgtcgctt gacggtggtg
     1381 tg
//
LOCUS       DAOTOW010000680         1381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_75570_length_1381_cov_3.306184, whole genome shotgun sequence.
ACCESSION   DAOTOW010000680 DAOTOW010000000
VERSION     DAOTOW010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75570_length_1381_cov_3.306184"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..807
                     /gene="fabG"
                     /locus_tag="P5243_10395"
     CDS             61..807
                     /gene="fabG"
                     /locus_tag="P5243_10395"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRS19905.1"
                     /translation="MKLLEGKTALITGGSRGIGKAIALGYAAQGCNIAFTDLVINDDA
                     LALVKELESYGVKAKGYASDASSFSSSHDAVAAILADFGTIDILVNNAGITKDSLLMR
                     MTEEQWDAVINVNLKSAFNLTKAVQPIMLKNRKGSIINVSSVVGIGGNAGQANYSASK
                     AGMIGFTKSIAKELGSRNIRCNAIAPGFIMTEMTAKLPENVIEDWKKRIPLNRGGKPE
                     DVANVCVFLGSELSDYVTGQVIPICGGLEM"
     gene            complement(862..>1381)
                     /locus_tag="P5243_10400"
     CDS             complement(862..>1381)
                     /locus_tag="P5243_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19906.1"
                     /translation="QLVDFDAIANENYIEIIWTTATETNNDYFTVERSTDGIHWVTIK
                     KVAGAGNSNTLQEYSVYDYEFVSDIAYYRLKQTDYDGTTTYSKIITVEAHRPQLQIQI
                     RYRDNQIVIDCPQELKQHIQCVELISISGAVVYKQNTFVPVIHTQGIAHGVYYVYCKH
                     KKTYTTERVIIE"
BASE COUNT          488 a          205 c          234 g          454 t
ORIGIN      
        1 aacgtaataa aaaataaatt atatatttga accaattaaa aaaataaaaa acaataaaca
       61 atgaaattac tcgaaggaaa aacagcactt attaccggtg gttctcgcgg tataggaaaa
      121 gcaatagctt taggttatgc agctcaaggt tgcaatatag cttttaccga tttagtaata
      181 aatgacgatg ctcttgcatt agtaaaagaa ttagaaagtt atggcgtaaa agcaaaagga
      241 tatgcttctg acgcaagttc attttcatct tctcacgatg cagtagcagc aattttagca
      301 gactttggca caattgacat tttagtaaat aatgccggta ttactaaaga ttcgttactt
      361 atgcgtatga cagaagaaca atgggatgct gtaataaatg taaatctaaa atcagcattt
      421 aatttaacaa aagctgttca acctattatg ttgaaaaaca gaaaaggttc tattataaat
      481 gtaagttctg tagtaggtat tggaggaaat gcaggacaag ctaattattc ggcatctaaa
      541 gccggcatga ttggtttcac taaatctatt gcaaaagaat tgggctcacg aaatattcgt
      601 tgcaatgcta tagctccggg atttataatg accgaaatga ctgcaaaatt acctgaaaac
      661 gtaattgaag attggaaaaa acgaattcca ttgaacagag gaggaaaacc tgaagatgtt
      721 gctaacgtat gtgtattttt aggttcagaa ttatcagatt atgttacagg acaagttatt
      781 ccaatatgcg gtggacttga aatgtaaaag aatgaatata aaaaaaccag gaaagggtgt
      841 tatatgtata acaccctttt tttattctat tataactctt tcagtagtat atgttttctt
      901 atgtttacaa tacacataat aaacaccatg tgcaatacct tgtgtatgta ttacaggcac
      961 aaaagtattc tgtttataca ctacagctcc actaatactt attaattcga cacattgaat
     1021 atgctgtttt aattcttgtg ggcaatcaat tactatttga ttatctctat atcgaatttg
     1081 aatttgtaat tgtggacgat gtgcttctac agttataatt tttgaatatg ttgtagtgcc
     1141 atcataatcc gtttgtttta atctataata tgcaatatca gatacaaatt cataatcata
     1201 cacagaatat tcttgcaagg tatttgaatt gcccgctcct gcaacttttt taattgttac
     1261 ccaatgaata ccgtcagttg aacgttcaac ggtaaaataa tcattatttg tttcggttgc
     1321 agtagtccaa attatctcta tgtagttttc attagcaatt gcatcaaaat cgactaattg
     1381 t
//
LOCUS       DAOTOW010000681         1379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_75737_length_1379_cov_3.419184, whole genome shotgun sequence.
ACCESSION   DAOTOW010000681 DAOTOW010000000
VERSION     DAOTOW010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75737_length_1379_cov_3.419184"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="P5243_10405"
     CDS             <1..782
                     /locus_tag="P5243_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRS19907.1"
                     /translation="IGVGQYQHDVNQHELQKKLDFVVESCVNLVGVELNTASKHLLQY
                     VSGIGPVLAENIVEYRKQHGAFATRLDILNVPKMGKKSFEQCAGFLRIRNGKHPLDNS
                     AVHPERYALVEQMALDVNANVFDLLNEDSIRKSINIHKYITNEVGEPTLLDILKELEK
                     PGRDPRKKLSSFAFDSSLKTIHDVKIGGIYPAIVTNITNFGAFVDIGIKQNGLVHISH
                     ICKEFITNPAQVLSINQTVKVKVLEVDINRNRLQLSIKDAH"
     gene            772..>1379
                     /locus_tag="P5243_10410"
     CDS             772..>1379
                     /locus_tag="P5243_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRS19908.1"
                     /translation="MLTNNSIPFVNIHSHNADVTDDICIYNCNDISEVELYFNRCSYG
                     LHPWNVQSYQQIDTFCHELELLLQKRNLLCVGECGLDITKGIDFELQLYAFSKQVELA
                     QRFNVPIIIHCVRAYYEIISILKKQRCTKPVIFHKFSGNSQIYSMCLKYDAYISLGPE
                     LCKHKSKHSVDFDFRLDRLLFETDTNSDVNISDVYNCFSEIY"
BASE COUNT          491 a          177 c          237 g          474 t
ORIGIN      
        1 caataggagt agggcaatat caacacgatg taaaccaaca tgaattacaa aaaaaattag
       61 attttgttgt agaaagttgt gttaatttag ttggagttga attaaatact gcaagcaaac
      121 atcttttaca atatgtatcg ggtattggtc ctgtattagc agaaaatata gtagaatata
      181 gaaagcaaca tggagctttt gcaacaagat tagatatatt gaatgttcca aaaatgggca
      241 aaaaaagttt tgagcagtgt gcaggttttt tgagaattcg aaatggcaaa cacccattag
      301 ataattctgc agtacaccct gaacgctatg ccttggttga acaaatggca ttagatgtaa
      361 atgcgaatgt ttttgattta cttaatgaag attctattcg caaatcaatc aatattcaca
      421 agtatataac taatgaggtt ggtgagccta cattacttga tattttaaaa gaattagaga
      481 aaccggggcg tgacccgcgt aagaaacttt catcatttgc atttgattca tcacttaaaa
      541 ctatacacga tgtaaaaata ggaggtatat atccggcaat agttacaaat attacaaatt
      601 ttggtgcttt tgtagatatt ggaatcaaac aaaatggttt agtgcatata tctcatattt
      661 gtaaagaatt tattacaaat cccgctcaag tacttagtat caatcaaact gttaaggtta
      721 aggttttgga agttgatatc aacagaaatc gtttgcaatt atcaatcaaa gatgctcact
      781 aataattcta taccatttgt aaatattcat tcgcataatg cagatgttac cgatgatatt
      841 tgtatatata attgtaacga tatttccgaa gtagagctgt atttcaatcg ttgttcgtat
      901 ggacttcatc catggaatgt gcaatcatat caacaaattg ataccttttg ccatgagtta
      961 gaactattat tacaaaagcg taatttgtta tgtgttggtg aatgtggttt agatattaca
     1021 aaagggatag attttgaatt gcaattatat gcattttcaa aacaagtaga gttagctcag
     1081 aggtttaatg tgcctataat aattcattgc gtaagagctt attatgaaat tatttcaatt
     1141 ctaaaaaaac aaaggtgtac caaacctgtt atttttcata aattttcagg taattctcaa
     1201 atatattcca tgtgtttaaa gtatgatgca tatatttcat taggaccaga attgtgtaaa
     1261 cataaatcta aacatagtgt tgattttgat tttcgattag atagattgtt atttgaaaca
     1321 gatacaaatt ctgatgttaa tattagtgat gtatataatt gtttttctga aatttacaa
//
LOCUS       DAOTOW010000682         1377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_75914_length_1377_cov_2.788956, whole genome shotgun sequence.
ACCESSION   DAOTOW010000682 DAOTOW010000000
VERSION     DAOTOW010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75914_length_1377_cov_2.788956"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="P5243_10415"
     CDS             <1..211
                     /locus_tag="P5243_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19909.1"
                     /translation="ELKDSLCEGLVSIRDLQDDQYIFDEKNYCLRGKYKNKVYTLGDD
                     VTVTIARADLAKRQLDYVIAEKDSE"
     gene            complement(300..800)
                     /locus_tag="P5243_10420"
     CDS             complement(300..800)
                     /locus_tag="P5243_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19910.1"
                     /translation="MKKLLCIAALFSAVYFTSCSGDVEPETSQKGFIIDTVGHFGYDF
                     SAAKMDTSYWETNMNDGGVIGWHSGRGTHPQYPSSSHYVWFRNSEIDTVHYECQIKNY
                     GTTDITKIHSINTNWDTIINPLIPGHVFGARCKDGYVLFEVVEVTDTLMWEAVLKFKF
                     SPTNNF"
     gene            complement(864..>1377)
                     /locus_tag="P5243_10425"
     CDS             complement(864..>1377)
                     /locus_tag="P5243_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19911.1"
                     /translation="NTPIDMTIKNVFPIAICIVMFLFSCKKEEATSGKIIVGIDTIYA
                     YGFDFSKGKCDTTRDVSKNTNDGKIVTSHPGTMNVAIPDDGGYIWFSNKDIHEEHMCQ
                     IKCYGEADMDTIHTINTNWDSFIDPLTNGHVYGAKCRDGYVLYKVKQVICPNLKIIVL
                     EYTFNTKNSF"
BASE COUNT          477 a          240 c          196 g          464 t
ORIGIN      
        1 tgaacttaaa gatagcttat gcgaaggatt agtttctata agagacctgc aagatgatca
       61 atatatattt gacgagaaaa attattgctt acgtggcaaa tacaaaaata aagtatacac
      121 acttggcgac gatgttactg taaccatagc aagagctgat ttagcaaaac gacaactaga
      181 ttatgtaata gctgaaaaag atagtgagta accatatgat ttttgtgaca tatactctat
      241 aaatacacaa caaaaatggg gacttttggt ccccattttt tatttgataa agcgtataat
      301 taaaaattat tagttggact aaatttaaac ttaagtaccg cttcccacat cagcgtatcg
      361 gtaacttcta caacttcaaa caatacatat ccgtctttgc aacgagcacc aaacacatgt
      421 cccggaatca acgggtttat aatagtatcc caattggtat tgatagaatg tatttttgta
      481 atatctgttg ttccataatt tttaatttga cattcataat gtacagtatc tatttctgaa
      541 tttcggaacc acacataatg cgaagaactc ggatattgag gatgtgttcc acgccccgaa
      601 tgccaaccta ttactccccc atcattcata tttgtttccc aatacgaggt gtccattttg
      661 gcagcagaaa aatcatatcc aaaatgacca acagtatcaa taataaaacc tttttgagaa
      721 gtttcaggct ctacatcgcc gctacaagaa gtaaaatata ctgcagaaaa taaagcagca
      781 atacataaca attttttcat aattcataag tataagttaa acaatataca aagatatata
      841 ttctgaataa ttttatataa aaactagaaa gagtttttag tattaaatgt atattcgaga
      901 actataattt ttaaatttgg acatattact tgttttacct tatacaatac atatccatct
      961 ctgcatttag ccccgtaaac atgtccattt gttaacggat ctataaaact atcccaattg
     1021 gtattgattg tgtgaatagt atccatatca gcttcgccat aacatttgat ttggcacata
     1081 tgctcttcat gtatatcttt atttgaaaac catatatatc caccatcatc gggaattgct
     1141 acattcatag tcccggggtg acttgttact attttaccat catttgtatt tttgcttaca
     1201 tctcttgtag tatcacattt accttttgaa aaatcaaatc cataggcata tattgtatct
     1261 atacctacaa ttattttacc ggatgtagct tcttcttttt tgcatgaaaa aagaaacatt
     1321 actatgcaaa ttgcaattgg gaatacattt ttaattgtca tatcaatagg ggtattt
//
LOCUS       DAOTOW010000683         1371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_76458_length_1371_cov_2.892857, whole genome shotgun sequence.
ACCESSION   DAOTOW010000683 DAOTOW010000000
VERSION     DAOTOW010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76458_length_1371_cov_2.892857"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="P5243_10430"
     CDS             complement(<1..618)
                     /locus_tag="P5243_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19912.1"
                     /translation="MTKKITILWADDEIELLKPHIIFLEAKGYEVIPVNNGIAAVEYC
                     KNQQFDIIFLDEQMPGLSGLDTLVQIKSIHPHIPIVMITKSEAEDIMEAAIGSQINDY
                     LIKPVNPNQILLSIKKNIQQTEIITQKTTMNYQQEFSQLGILIMQAETWNDWVEIFKK
                     LTQWEIQLTRSNDRGMDEVLRMQKNEANTNFAKCIKKNYIQWIQSN"
     gene            complement(651..>1371)
                     /locus_tag="P5243_10435"
     CDS             complement(651..>1371)
                     /locus_tag="P5243_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19913.1"
                     /translation="ELEKTYADSYRLFVKGDYKGVILLHNQQEKFFKDTYLQAKFDFL
                     KAVSEGKLYGIPTLQTNMQNIINTYPKDAVSTLAKTILDYISKEQNQKTITQPVIDIV
                     SNNSVKEEQKKVPDVYVYPSTPTTHIYVIAAKSEFIDMKKLKFNIVNYNLDYFTNFNF
                     SIEQKDLDATYSIIAVSPFQNANQAMNYFDLINYNQEIYENIEKVFTRHCIISVDNYK
                     TLLQDRNIETYLKFFDEYYIR"
BASE COUNT          406 a          183 c          196 g          586 t
ORIGIN      
        1 attagattga atccattgga tatagttttt tttgatacat ttagcaaaat tggtatttgc
       61 ctcatttttt tgcatacgaa gaacttcatc cattccacga tcatttgagc gtgtaagttg
      121 aatttcccat tgcgtgagtt ttttaaatat ttcaacccaa tcattccatg tttctgcttg
      181 cattataaga attcccaatt gtgaaaattc ttgttgataa ttcattgttg ttttttgtgt
      241 aataatttcg gtttgttgga tattcttttt aatcgaaagc agtatttggt tgggatttac
      301 cggttttatt aagtagtcat ttatttgaga gcctatagca gcttccatta tatcttcagc
      361 ttcacttttg gtaatcatta caatgggtat atgtggatgt atagatttaa tttgaactaa
      421 tgtatctaat ccacttaatc ccggcatttg ttcatctaaa aatataatat caaattgttg
      481 gtttttgcaa tattcaacag ctgcaattcc attatttaca ggaattactt catatccttt
      541 agcttcaaga aaaataatat gaggcttgag taattcaatt tcatcatcag cccataatat
      601 ggttattttt tttgtcatgc ttacaaagta ttttttgagt aacgtaacta ttagcgaata
      661 tagtattcgt caaagaattt taagtacgtt tctatattac ggtcttgcaa aagtgttttg
      721 taattgtcaa cagatataat gcaatgacgt gtaaacactt tttcaatatt ttcataaatt
      781 tcttggttgt agtttatcaa atcaaaataa ttcattgctt gatttgcatt ttgaaaagga
      841 cttacagcta ttatactata tgttgcatca aggtcttttt gttcaattga aaaattaaaa
      901 ttggtaaaat agtctaaatt atagtttaca atattaaatt ttagtttttt catatcaata
      961 aattcagatt ttgctgctat tacatatata tgtgtggtag gagtagatgg atacacatat
     1021 acatcaggaa cttttttttg ttcttctttt acactgttat tcgatacaat atcaattaca
     1081 ggttgtgtta ttgttttttg attttgttct tttgaaatat agtctaatat agtttttgct
     1141 aatgtactta ctgcatcttt agggtatgtg tttattatat tttgcatatt tgtttgcagt
     1201 gttggtatac catataattt accttcactt acagctttta aaaagtcaaa ttttgcttgt
     1261 aggtatgtat ctttaaaaaa tttttcctgt tgattatgta atagtattac acctttataa
     1321 tctcctttta caaaaagcct ataactatca gcatatgttt tttctaattc t
//
LOCUS       DAOTOW010000684         1371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_76470_length_1371_cov_2.541033, whole genome shotgun sequence.
ACCESSION   DAOTOW010000684 DAOTOW010000000
VERSION     DAOTOW010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76470_length_1371_cov_2.541033"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="P5243_10440"
     CDS             <1..447
                     /locus_tag="P5243_10440"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRS19914.1"
                     /translation="YYPNTQEVEQMVSQKGPYSFYTSVENRKECCTIRKVKPLNRALE
                     GKQIWITGIRAEQSPNRQDLQMFEFDDERQLIKCNPLINWTFTQVQEYIKKNQIPYNS
                     LHDKGFVSIGCEPCTRAIQPGEDFRAGRWWWEDNSKKECGLHTHKK"
     gene            453..>1371
                     /locus_tag="P5243_10445"
     CDS             453..>1371
                     /locus_tag="P5243_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRS19915.1"
                     /translation="MKKIAVIVILCSFCFVSSMFANISLPSFFSSNMVLQQNADVLIW
                     GWAIPDEEIKIQIGWDDTVYVTKTLSDSTWQIQIQTPKAGGPYTIRFQGYNSLLLQNI
                     LIGEVWICAGGSNMEWSAKMGINNADREIAAANYPEIRMFTVAKQASGVKQKNVQGRW
                     NVCSPQIMANYSAAAYFFARELQKELNVPIGIIQATWPESAAEVWTPYDAIFTNRKIA
                     DASLKLKDNKTCTTIPGQLYNGMIAPLAPLKIKGFIWYQGEANVVNARTYDLLMAALI
                     EGWRDAWDEDLPFYFVQIAPYDKYEPETGV"
BASE COUNT          519 a          224 c          259 g          369 t
ORIGIN      
        1 tattatccaa atacacaaga agtagaacaa atggttagcc agaaaggtcc atacagtttt
       61 tatacttcag tagaaaaccg taaagaatgc tgtactattc gtaaagtaaa accactaaac
      121 agggcattgg aaggaaaaca aatttggata acaggaatta gagcagaaca atcaccaaac
      181 agacaagatt tacaaatgtt tgaatttgat gatgaaagac aactaataaa atgtaatcca
      241 ctcataaatt ggacatttac acaagtacaa gaatacatta aaaaaaatca aattccatac
      301 aatagtcttc atgacaaagg atttgtaagc ataggatgtg aaccgtgtac acgagctata
      361 caaccaggtg aagactttag agcaggacga tggtggtggg aagataattc taaaaaagaa
      421 tgtggccttc atactcacaa aaaataaaac atatgaaaaa aatagcagta attgtaattc
      481 tttgtagttt ttgtttcgta tcgtctatgt ttgcaaatat tagtttgcct tcattttttt
      541 caagtaatat ggtattacaa caaaatgccg atgtactaat atggggttgg gcaattcccg
      601 acgaagaaat aaaaattcaa ataggttggg acgatactgt gtatgtaacc aaaacattat
      661 cagattctac gtggcaaatt caaattcaaa ctcccaaagc aggaggcccc tacactattc
      721 gttttcaagg atataattca ttactgcttc aaaacattct aataggtgaa gtatggatat
      781 gtgccggagg aagcaacatg gaatggtcag caaaaatggg aataaataat gctgacagag
      841 aaatagcagc agcaaattac cccgaaatta gaatgtttac cgtagcaaaa caagcttcgg
      901 gggtaaaaca aaaaaatgta caaggtcgtt ggaatgtatg ttctcctcaa ataatggcca
      961 attatagtgc cgcagcatat ttttttgcac gcgaattaca aaaagaatta aatgtgccta
     1021 ttggaattat acaagctact tggcccgaat cggcggcaga agtatggacc ccttatgatg
     1081 caatatttac aaacagaaaa attgcagacg cctcattaaa actcaaagat aataaaacat
     1141 gtacaaccat acccggacaa ttatacaatg gtatgatagc accacttgca ccattaaaaa
     1201 taaaaggatt tatatggtat caaggcgagg caaatgtggt aaatgctcga acttatgatt
     1261 tacttatggc tgctcttatt gaaggttggc gtgatgcttg ggacgaagat ttaccctttt
     1321 attttgttca aatagctccg tatgataaat atgaaccaga aaccggtgtt t
//
LOCUS       DAOTOW010000685         1367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_76838_length_1367_cov_2.238567, whole genome shotgun sequence.
ACCESSION   DAOTOW010000685 DAOTOW010000000
VERSION     DAOTOW010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76838_length_1367_cov_2.238567"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="P5243_10450"
     CDS             complement(<1..1311)
                     /locus_tag="P5243_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRS19916.1"
                     /translation="MKSVINQSKMLIKNIYKKYVYLEYFFMSIFFISITLNPLYSQMK
                     VIGQDLYTQDNEKVVLRGINEMFIWSGDKTGKNTLPEIAKTGANACRLVWNISGSTTD
                     LEALIDNCIKNKMIAIPEMHDATGPAISTKFQPIIDYWTKPEVVAIIKKHERFTIVNI
                     ANEAGSSITIQEWVNYYKPAITALRNAGIKTPLMIDCAGYGNWEGFFLGGGQELINHD
                     PEHNIIFSVHTYWLTTTGPDANKTRLDKLISDAKAKNLPWCVGEGPQMVESPGGGGCT
                     RPFPYIYMIDRLQQEGIGWLSWSWGLVNNSDCGSPNSAFDMTTDGKFGNWATTFAEEI
                     CISATNSIKNTSIRPASLVGGTIINVTGVSLNQTSITLDPNKTATLTPTVLPTNANNK
                     SVTWKSNNTSVATVNSSGVVTAIAPGDAIITVTTVDGGFIATCSV"
BASE COUNT          413 a          247 c          224 g          483 t
ORIGIN      
        1 aaccgagcaa gttgcaataa aacctccatc taccgtagtt acagtaatta ttgcatcacc
       61 cggagcaatt gctgttacta cgccggaact attaacggtt gcaactgatg tgttatttga
      121 tttccaagta acacttttat tatttgcatt tgttggcagt acagtaggtg ttaatgtagc
      181 tgttttgttt gggtcaagag taatacttgt ttgatttaat gatacaccgg taacatttat
      241 aattgttcct ccaactaacg aagcgggtcg aatagatgta ttttttatag aattagtggc
      301 tgaaatacaa atttcttcgg caaatgtagt agcccaattg ccaaatttac catcggtagt
      361 catatcgaaa gcactattgg gagaaccaca gtcgctatta tttacaagtc cccatgacca
      421 tgaaagccat ccaatacctt cttgttgtaa tctatctatc atataaatat acgggaaagg
      481 gcgtgtacaa ccacctcctc ccggagattc aaccatttga ggaccttcgc caacacacca
      541 tggtaaattt tttgctttag catcagatat cagtttatct aaacgggttt tattagcatc
      601 tggtccggtg gtggttagcc aatacgtatg tacagaaaaa ataatattat gttcgggatc
      661 gtgatttatt agttcttgcc cgccacctaa aaagaaacct tcccaatttc cgtatcctgc
      721 acagtctatc attaacggtg tttttattcc tgcattgcga agagcagtta ttgccggttt
      781 ataataattt acccactctt gtatagttat acttgagccg gcttcgtttg ctatattaac
      841 tatagtaaac cgttcatgtt tttttattat agctacgact tcgggttttg tccaataatc
      901 tatgattggt tgaaattttg tagatattgc aggaccagta gcatcgtgca tttcgggaat
      961 tgctatcatt ttatttttta tacagttatc tattaatgct tcaagatcgg tagtagaacc
     1021 ggaaatattc caaactaacc gacacgcatt tgctccagtt tttgcaattt caggcaaagt
     1081 gtttttgccg gttttatccc cactccaaat aaacatttca tttatacctc ttaaaacaac
     1141 tttttcattg tcttgtgtgt aaaggtcttg tcctattact ttcatttgac tatataatgg
     1201 atttaaagta atgcttataa aaaatatcga cataaaaaaa tactctaaat acacatattt
     1261 tttataaatg tttttaatta acatttttga ttgattaatt acagatttca taatgataag
     1321 aattttaaaa attacttatc aaaagtagat tatgcataca gtaaata
//
LOCUS       DAOTOW010000686         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_77003_length_1365_cov_3.263359, whole genome shotgun sequence.
ACCESSION   DAOTOW010000686 DAOTOW010000000
VERSION     DAOTOW010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77003_length_1365_cov_3.263359"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="P5243_10455"
     CDS             <1..1257
                     /locus_tag="P5243_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRS19917.1"
                     /translation="GIIEFFKSKFKGDKVIWRLLFILSAISLLAVYGSISLIAIKGNE
                     NNTTIFLLKQAFTLGVGIAITYWVHNLDYSWFFKLSNILIILSAIFLMLTFSSTFAVE
                     TNNANRWIQFPGIGLRFQPSEFAKIALLIFIAKVLSIHQKDLGNTKKVLYPILIVTLV
                     ISGMVLISNFSTAFFILFTTVVMLFFGRIPWKQMFALAGVGAFVGILMLGIILKAPDL
                     FPRGGTWQKRIVSFMPQLASQAKGYDASKEASEIIVDNHQVTQAKIAIAEGGILGKAP
                     GNSSQKYKLSQAYCDFIYAIIIEDYGLIGGFIIMGLYLILMYRAGRIVKKCEYTFPAL
                     LVIGLTFSMVFQAFVHMGVAVNLLPATGQTMPIISLGGTSLLTTSISFGIILGISRKI
                     DAEEINKTKEKSNKETEDIHKTQEVA"
     gene            1254..>1365
                     /locus_tag="P5243_10460"
     CDS             1254..>1365
                     /locus_tag="P5243_10460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS19918.1"
                     /translation="MKVIISGGGTGGHIFPAVSIADELIKRVPHVEILFVG"
BASE COUNT          458 a          182 c          270 g          455 t
ORIGIN      
        1 ggaattatag aatttttcaa aagcaaattt aaaggcgata aggtaatatg gcgcttgctg
       61 tttatattat cggctatttc attgcttgct gtgtatggtt ctataagtct tattgctata
      121 aaaggaaacg aaaataacac aactatattc ttgcttaaac aagcatttac acttggcgta
      181 ggaatagcaa ttacgtattg ggttcataat ttagattatt cgtggttttt taaattatca
      241 aatattttaa taattctgag cgctattttt cttatgctta cattttcaag cacatttgcc
      301 gttgaaacaa ataatgcaaa tcggtggatt cagtttccgg gtattggatt gcgttttcaa
      361 ccaagtgaat ttgcaaaaat tgcattactc atttttattg ctaaagtact ctctatacat
      421 caaaaagatt taggtaatac taaaaaagta ttgtatccaa ttcttatagt tacccttgtg
      481 attagtggca tggtacttat ctcaaatttt tcaacagcat tttttattct ttttactacg
      541 gtagtcatgc tgttttttgg aagaattcca tggaaacaaa tgtttgcatt ggcaggagta
      601 ggtgcatttg taggaattct aatgcttgga attatattaa aggcacccga tttatttcct
      661 cgtggaggta catggcaaaa acgtatagtg agtttcatgc cgcaattagc atcgcaagca
      721 aaagggtatg atgcttcaaa agaagcaagt gaaataattg tagataatca tcaggtaacg
      781 caggctaaaa tagcaattgc agaaggaggt atattaggta aagcaccggg aaatagtagt
      841 caaaaatata aattatctca ggcatattgt gattttatat atgctatcat aattgaagat
      901 tatgggttaa taggtggttt tataattatg ggattatatt taattcttat gtatagagcc
      961 ggaagaattg taaaaaaatg tgaatatacc tttcctgcat tgttggttat tggactcaca
     1021 tttagtatgg tgtttcaagc atttgtacat atgggtgttg ctgtaaattt attgccggca
     1081 acggggcaaa ctatgccaat tataagttta ggaggaacat cattgcttac cacaagtata
     1141 tcatttggaa taatactggg aattagcaga aaaatagatg ctgaagaaat aaataaaaca
     1201 aaagagaaat ctaataaaga aacagaagat atacacaaaa cacaggaggt tgcatgaaag
     1261 taataataag cggtggaggt accggcggac atatatttcc ggcagtttca attgccgatg
     1321 agcttattaa acgagttcct catgttgaaa tattgtttgt gggag
//
LOCUS       DAOTOW010000687         1361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_77370_length_1361_cov_2.188361, whole genome shotgun sequence.
ACCESSION   DAOTOW010000687 DAOTOW010000000
VERSION     DAOTOW010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77370_length_1361_cov_2.188361"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..>1361
                     /locus_tag="P5243_10465"
     CDS             433..>1361
                     /locus_tag="P5243_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRS19919.1"
                     /translation="MADFCKKTGGECYGIRELNLLLEISTILNKPTHNLKENLQLVVE
                     KLAQYLHAERVILTILNRTNSQIYIEVGFGLTAAEQDRGTYSIGEGIIGKVVETGTPI
                     VIPRIRDDDTYLNKTGTVSKIPNAEVSFICVPIKNESEIVGTLSIHRIYNRHITFAED
                     ARILSIIGSMVAQSVRNRQQLLEEIEHLQDENRQLQSQLKERIVPKGIVGNSSKMAEV
                     FDLIEKVAPTNATVLIRGESGVGKELIADAIHFGSDRRHKPFIKVNCAALPDSLIESE
                     LFGHEKGAFTGANILRKGRFEAAEGGTIFLDEIG"
BASE COUNT          483 a          165 c          251 g          462 t
ORIGIN      
        1 tatttttact attgggataa caataaatta tattcgttta ccatgtttcg ctttttgcca
       61 agtatttctt atggttttga tttttgagaa taaaatttat agatagtagt aagggtaaca
      121 tataacaata ctaacaaagg agaagtaagt tgtttacaaa tatttgcaac atgcgatggt
      181 tatagtttaa aacagtattg caaaaatgag ttagcaaaca taatccttgg tttttttaag
      241 aatttatgaa ttaaaagagg ttttgtttag caatcaattt tttattcata ttcctattgt
      301 gtaataaaat aaataagaag tttttatcca catcatttaa ttaggtaatc tctttatttt
      361 ttttgcgtaa ttctcgtata gaatacagtt gcatagtata ctatttttga atactaatga
      421 gaaataacta ctatggcaga tttttgtaag aaaaccggtg gcgaatgcta tggaatacga
      481 gaattgaatt tattacttga aataagtaca atactaaata aaccaactca taatttaaaa
      541 gagaatttac aacttgttgt tgaaaaatta gctcaatatt tacatgcaga gagggttata
      601 cttactattc ttaatagaac taattcacaa atttatattg aggtaggttt tgggttaact
      661 gctgccgaac aagatcgtgg aacatatagt ataggcgaag gaattattgg taaagttgtt
      721 gaaaccggta ctccaattgt aattcctcgc atacgtgatg atgatacgta tttgaataaa
      781 acaggaactg tttcaaaaat tccaaatgct gaagtgtctt ttatctgtgt acctataaag
      841 aatgaaagtg aaattgtagg tactttaagt attcatagaa tatataatag acatattact
      901 tttgcagaag atgcaagaat tctctctatt ataggttcta tggttgctca atctgttcgt
      961 aatcgtcagc aattacttga agaaattgaa catcttcaag atgaaaacag gcaattgcaa
     1021 tcgcaactta aagaacgaat tgttcccaaa ggaattgtgg gaaattcgag taaaatggct
     1081 gaggtattcg atttaattga aaaagttgca cctactaatg ctacagtatt aataagagga
     1141 gaaagtggtg ttggtaaaga attaattgca gatgctattc attttggaag tgaccgtagg
     1201 cataaacctt ttataaaagt aaactgtgca gcattaccag acagtttaat tgaaagtgaa
     1261 ctatttggtc atgaaaaggg ggcatttacc ggagctaata ttttgcgtaa agggagattt
     1321 gaagcagccg aaggtggtac aatttttctc gatgaaatag g
//
LOCUS       DAOTOW010000688         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_77416_length_1360_cov_3.789272, whole genome shotgun sequence.
ACCESSION   DAOTOW010000688 DAOTOW010000000
VERSION     DAOTOW010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77416_length_1360_cov_3.789272"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="P5243_10470"
     CDS             complement(<1..315)
                     /locus_tag="P5243_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19920.1"
                     /translation="MQFEIIQPHYLMLLCDNDTQFMCKMYEAFLQQNTNVQEQLQECI
                     IHFDVIRAKKIIHTLKSSFSILGVDVCKTEIEITESDSFLSIKKIEFIRIIKKILFVY
                     IQM"
     gene            complement(336..>1360)
                     /locus_tag="P5243_10475"
     CDS             complement(336..>1360)
                     /locus_tag="P5243_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19921.1"
                     /translation="IFLVDKLLRFTRDLNTLLRNFVTFHDLYAPHARAIFEVGTLFIE
                     QRSCNLCIQVSDMAKHNTLAGYSGMFLIYCDCASKTLHKQMTIVVGLTNGDIDNVMVG
                     RNAVFYDRDGNDWDATIIKIIENPISIRQAFWSPYRKVARFIETQANKFAASKENKVT
                     SQVTNSIEKTTNEAESKNVMEISAPPAGTQPQPFDIGKFVGIFAAIGMAIGAIGAVLA
                     SFISGFLSLSWWKMPLAIIAIILVISFPSMFIAWLKLRKRNLAPILDANGWAINARVL
                     INIPFGNTLTQLVKLPKNSKIDLKDPFEKTTTPVWLKITYVIVCLGIIVALLWYFGYF
                     VKWGWI"
BASE COUNT          468 a          229 c          175 g          488 t
ORIGIN      
        1 catttgtata taaacaaaca gtattttttt tatgattctt ataaattcta tttttttgat
       61 agacaagaat gaatctgatt ctgtaatttc aatttctgtt ttacacacat caacaccaag
      121 aatactaaac gatgatttga gtgtatgaat aatttttttt gctcgtatta cgtcaaaatg
      181 tataatacat tcctgtagtt gttcttgtac gtttgtattt tgttgaagaa atgcctcata
      241 cattttacac ataaattgag tatcattatc acataacaac atcaagtaat gtggttgaat
      301 aatttcaaat tgcatatttt aaaataattt gaaagttata tccatcccca ttttacaaaa
      361 taaccaaaat accataacaa agcaactata attcctaaac acactattac atacgttatt
      421 ttaagccaaa ccggagttgt tgttttttca aatggatctt taagatcaat tttagaattt
      481 ttaggcaatt ttactaactg tgttaatgta tttccaaaag gtatatttat aagaacacga
      541 gcatttatag cccatccatt tgcatctaaa atcggagcta aattacgttt tcgtaatttt
      601 aaccatgcaa taaacattga ggggaaagat ataaccaaaa taattgcaat tatagccaaa
      661 ggcattttcc accacgatag acttaaaaaa ccggaaataa acgaagctaa tactgctcct
      721 attgctccta ttgccatacc aatagcagca aaaatcccaa caaattttcc aatatcaaat
      781 ggctgtggtt gtgtacctgc aggtggagcc gaaatttcca taacattttt tgattcagct
      841 tcatttgttg ttttttctat tgagtttgta acttgacttg ttactttatt ttctttagat
      901 gcagcaaatt tatttgcttg agtttcaata aatcgagcta cttttctata gggcgaccaa
      961 aacgcttgac gaatacttat aggattttct attattttaa tgattgtagc atcccagtca
     1021 tttccatctc tatcataaaa tacggcgttt ctacctacca ttacattatc tatatcaccg
     1081 tttgtaagtc ctactacaat tgtcatttgt ttatgtaatg tttttgaagc acaatcgcaa
     1141 taaattaaaa acattccgct ataccctgct aaagtattat gttttgccat atcagaaact
     1201 tgaatacata aattgcaact acgttgctct ataaacaatg ttccaacttc aaaaatagct
     1261 cgtgcatgag gtgcatataa atcatgaaat gtcacaaaat ttcgtaataa agtatttaaa
     1321 tcacgtgtaa atctgagtaa tttatcaact aaaaaaatat
//
LOCUS       DAOTOW010000689         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_77699_length_1357_cov_2.811060, whole genome shotgun sequence.
ACCESSION   DAOTOW010000689 DAOTOW010000000
VERSION     DAOTOW010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77699_length_1357_cov_2.811060"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="P5243_10480"
     CDS             <1..198
                     /locus_tag="P5243_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19922.1"
                     /translation="EMAEGYITKIGLDAGNEIIGYEFVNLGRMMDMIKSGVDANEAIK
                     KASGKYGRIEDAVKVIDPRKE"
     gene            212..1213
                     /locus_tag="P5243_10485"
     CDS             212..1213
                     /locus_tag="P5243_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HRS19923.1"
                     /translation="MEQQLFESYSRRINQINKVLNSYGISSIQEAKEICDKKGVDVYQ
                     IVKDIQPIAFENAMWAYIVGAAIAIKKGQTNAADIAATLGEGLQAFCIPGSVADDRKV
                     GIGHGNLAAMLLREETKCFAFLAGHESFAAAEGAIGIAKSANRVRKEPLRVILNGLGK
                     DAAQIIARINGFTYVQTQFDYATGEVKEVKRIAYSTGDRAKVNCYGADDVREGVAIMH
                     KEGVDVSITGNSTNPTRFQHPVAGTYKKECVEQGKKYFSVASGGGTGRTLHPDNMAAG
                     PASYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVALSQAK"
BASE COUNT          457 a          215 c          293 g          392 t
ORIGIN      
        1 gaaatggctg aaggttatat aaccaaaatc gggttggatg ccggcaatga aattatcggt
       61 tacgagtttg taaatcttgg ccgcatgatg gacatgatta aatcaggcgt agatgctaac
      121 gaagccatta aaaaagcttc gggcaaatac ggtcgaatag aagatgcagt taaagttatt
      181 gacccacgta aagaataagg aggaatatac aatggaacaa caattatttg aaagttacag
      241 cagaagaatt aaccaaataa ataaggtgtt aaattcttat ggaatttctt ctattcaaga
      301 agcaaaagaa atttgtgata aaaaaggtgt agatgtatat caaatagtaa aagatattca
      361 acctatagct tttgaaaatg caatgtgggc ttatatagta ggtgctgcta ttgctattaa
      421 aaaaggacaa accaatgcag ccgatattgc agctactctt ggtgaagggt tacaagcatt
      481 ttgtattccc ggatcggtag ccgatgacag aaaagtaggt attggacatg gtaatcttgc
      541 agctatgttg ctacgcgaag aaactaaatg ttttgctttt ttggcaggtc atgaatcatt
      601 tgctgctgcc gaaggcgcta taggtatagc aaaatcagca aatagagtgc gtaaagaacc
      661 attacgagtt atattaaatg gtttgggtaa agatgctgca caaattattg cacgtatcaa
      721 cggatttact tatgtgcaaa cacaatttga ctacgctaca ggcgaagtta aagaagtaaa
      781 acgtattgca tattctaccg gtgacagagc aaaagttaat tgttatggtg ccgatgatgt
      841 acgtgaaggt gttgctataa tgcataaaga aggagttgat gtatctatta ccggtaactc
      901 aaccaatcca actcgttttc aacatccggt tgcaggtaca tacaaaaaag aatgtgttga
      961 gcaaggtaaa aaatatttct ctgtagcatc aggtggtgga acaggtcgta cattgcatcc
     1021 tgataatatg gcggcaggac ctgcttcata tggtatgaca gatacaatgg gacgtatgca
     1081 ctctgatgca caatttgccg gatcatcatc agtaccagct cacgtagaaa tgatgggatt
     1141 aataggaatg ggtaataacc caatggttgg tgcttctgtt gctgttgcag ttgctctttc
     1201 acaagcaaaa taattatcta acaaatattt ttataaaaaa agcccaatgt ataattgggc
     1261 ttttttttat gctttccaaa ttacacctcc acatacattt ttttgagcac ctgttacgct
     1321 tgctaaacaa ttgtgctttt cttgaatgcg taagaaa
//
LOCUS       DAOTOW010000690         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_78110_length_1352_cov_3.139553, whole genome shotgun sequence.
ACCESSION   DAOTOW010000690 DAOTOW010000000
VERSION     DAOTOW010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78110_length_1352_cov_3.139553"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..884
                     /locus_tag="P5243_10490"
     CDS             186..884
                     /locus_tag="P5243_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRS19924.1"
                     /translation="MIIINIIQMILLSLWTIFIVYILTPFFNYKGAWWITVNLWAPGI
                     AYILLTRIEVKGIENIDQKKHYIFMSNHASYFDIACLFIATKRNLHFLAKYELSRNFF
                     TGYMLRKLQIIFIDRTNSQRSAASLLKAVEHIQKGKNVAIFPEGTRTRTGKLGVFRKG
                     GFKLAVQSNTPIIPVSITNSAKAWPIHHFGFRPTTVTVTFGPEIQVTGIDENDTKELI
                     DSVYTFISNNTQNS"
     gene            896..>1352
                     /locus_tag="P5243_10495"
     CDS             896..>1352
                     /locus_tag="P5243_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRS19925.1"
                     /translation="MKLFNTIVFAITTNLYIIGCSNNDNPIVFSKITDDSKIILTLDT
                     NKTFTFTARHVKGAAFDESGTYTINDSTIILQYNNKEYSYNCYTIPLPNDTMLFGHYN
                     NTILLYPLYREIPGIDSVLTHEQVMQRIVNEYNSPKFKTHVGTLIFACTQ"
BASE COUNT          501 a          221 c          175 g          455 t
ORIGIN      
        1 acaatataat attgtatttt ttttataatc aacgtattat atattggaaa caacacgtcg
       61 ggagattgtt ctcccataaa actgggaatt aaatataatg ttcctgtcat tttttgttcc
      121 atattaattc tatatttcaa atgatttgat ttattttgta aaaatattta aaaatcatag
      181 aaattatgat aataattaat attattcaga tgatattatt gtcgttgtgg acaattttta
      241 ttgtgtatat cctcacccca ttttttaatt ataaaggagc atggtggatt actgttaatt
      301 tatgggcacc gggcattgca tatattctac tcacccgtat tgaagtaaaa ggaatagaaa
      361 atattgacca aaaaaaacat tacatattca tgtcaaatca tgcatcatac tttgatatag
      421 catgtttgtt tattgccaca aaacgcaact tacatttttt agcaaaatat gaactaagcc
      481 ggaatttttt cacaggctac atgctgcgca aactccaaat aattttcata gacagaacta
      541 attctcaacg ttcggctgca agtttactaa aagcagtaga acatatacaa aaaggtaaaa
      601 atgtagctat attccccgaa ggtacaagaa caagaaccgg aaaacttggt gtattcagaa
      661 aaggtggctt taaattagct gttcaatcaa acactccaat tattccggtt tctataacaa
      721 acagtgcaaa agcttggcct atacatcatt ttggatttag accaactaca gtaactgtaa
      781 catttggacc tgaaattcaa gtaaccggaa tagatgaaaa tgacacaaaa gaattaattg
      841 attctgtata tacttttata tcaaacaata cacaaaactc ttaattatat tttatatgaa
      901 attattcaat acaattgttt ttgcaattac aacaaatcta tacattatag gatgttcaaa
      961 taacgataat cctattgtat tctcaaaaat taccgatgat tctaaaatta ttcttacact
     1021 tgacaccaat aaaaccttta catttacagc aagacacgta aaaggtgcgg cttttgatga
     1081 gtctggtaca tacacaatta atgactctac tattatttta caatataata acaaagaata
     1141 ttcatataat tgttatacta taccactacc aaacgacact atgttatttg gccattataa
     1201 taatactatt cttttatatc ctctctatag agaaataccc ggcattgaca gtgttttaac
     1261 acacgaacaa gttatgcaac gaattgtaaa tgaatataat tctccaaaat tcaaaaccca
     1321 tgtcggcaca ttaatttttg cttgtactca ag
//
LOCUS       DAOTOW010000691         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_78485_length_1348_cov_2.720804, whole genome shotgun sequence.
ACCESSION   DAOTOW010000691 DAOTOW010000000
VERSION     DAOTOW010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78485_length_1348_cov_2.720804"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="P5243_10500"
     CDS             <1..212
                     /locus_tag="P5243_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19926.1"
                     /translation="IANTDYTTKRTIVTNSNLANPFTGYNFSHVHAYNTIYDNTILGG
                     EYIIPGSMIFDEKMYDYSNMFNCNP"
     gene            246..1115
                     /locus_tag="P5243_10505"
     CDS             246..1115
                     /locus_tag="P5243_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19927.1"
                     /translation="MFLKNDIISISTNPSPIPLITHKAKSQVDFIYELPEEYDNRYIY
                     LNGLKIEILDAYYNTDGNGKDYKIKVSWKNYTVQNNVRWTGNIILHEQVIIQSGNTVT
                     LDQGLSPTKSSKPQTINGTYVFADKTVFTCKSNSTITIEDGAKLILRNGSSLILENGA
                     KIIIKGSGQLVLECGTTLESKMYSYIELHDAVSSILEEIGSAATYWSIPGTGSIVGNG
                     KRIQFSGNTIVSNISYTTKSLEFGAIITTQGSVKIQSPQVIFTAKEVYLNSGFEVYPG
                     SSLILDTNNFNCQ"
     gene            1116..>1348
                     /locus_tag="P5243_10510"
     CDS             1116..>1348
                     /locus_tag="P5243_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19928.1"
                     /translation="MKYIFILLVIACVHVQAYAQTYQPILGKDSTQWNYVMYYDVEFS
                     DIERVTTHIYKAYGDTVINSIAYVRMYENDYKE"
BASE COUNT          519 a          171 c          226 g          432 t
ORIGIN      
        1 atattgcaaa tactgattat accacaaaaa gaacaattgt aacaaattca aatctcgcaa
       61 atccatttac cgggtacaat ttctcacatg tacatgcgta taatacaatt tatgataata
      121 caattttggg aggagaatac attattcccg gaagtatgat tttcgatgag aaaatgtatg
      181 attattccaa tatgtttaat tgtaatccat aatatccgat tacaataaat ggatcaaatc
      241 gtgatgtgtt tttaaaaaat gatattattt caatttctac gaatccatcg ccaatacctt
      301 taattacaca taaagctaaa tcacaagtag attttattta tgaattacct gaagagtatg
      361 ataatcgata tatatatttg aatgggttaa agattgaaat tttagatgct tattacaata
      421 ccgatggtaa tgggaaagat tataaaataa aagttagttg gaagaattat actgtgcaaa
      481 ataatgttcg ttggacagga aatataattc tacatgaaca ggttattata caatcaggga
      541 atactgtaac tttagatcag gggttatctc caacaaaatc atcaaaaccc caaacaataa
      601 atggaactta tgtatttgct gataaaacag tgtttacatg caagtcaaat tccacaataa
      661 cgatagaaga tggagctaaa ttaatattga gaaatgggag ttctttgata ctagaaaatg
      721 gggcaaagat aataattaaa ggttcggggc aacttgtgtt ggaatgtggt acaacattag
      781 agtcaaaaat gtatagttat atagagttac acgatgctgt aagttcaatt ttagaagaaa
      841 taggtagcgc tgccacatat tggtcaattc ccggtaccgg ttcaattgtg gggaatggaa
      901 aacgaattca attttcggga aatactatag tttcaaatat atcatataca actaaatcat
      961 tagaatttgg tgctataatt actacacaag gaagtgtaaa aattcaatca ccacaagtaa
     1021 tatttacagc taaagaagta tatttgaatt ctggttttga agtatatccc ggctcaagtt
     1081 taattttaga tacaaataat tttaattgtc aataaatgaa gtatatattt attttgcttg
     1141 taattgcatg tgtccatgtt caagcatatg ctcaaacata tcagccaata ttaggaaaag
     1201 attcaacaca atggaattat gttatgtatt atgatgttga attttcagat attgaaagag
     1261 taacaacaca tatttataaa gcatatggcg atacggtaat taacagtatt gcgtatgtta
     1321 gaatgtacga aaatgattat aaagaaaa
//
LOCUS       DAOTOW010000692         1346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_78663_length_1346_cov_2.422928, whole genome shotgun sequence.
ACCESSION   DAOTOW010000692 DAOTOW010000000
VERSION     DAOTOW010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78663_length_1346_cov_2.422928"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1346)
                     /locus_tag="P5243_10515"
     CDS             complement(54..>1346)
                     /locus_tag="P5243_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19929.1"
                     /translation="NGCSNTASKTITVNALPSVSVSIPAIVCKNGSNITPATTPSGGI
                     LKLDGSTITSIDPSSLSTGNHIVTYEYTDPTTNCTNTDTKNFTLVNINPPTVSNKNVE
                     INSSDLTITATGSGGTLTWRNSTNTVIGTGSTYTHPSSSSVGSWDYCVTESDGTCTSN
                     PACMTFTVTNCATPAPVVTNANYTICSGSTIPSFNTSTAGTIKWYNAANSVVFTGNSF
                     TPTVSNTVASTHTWKVTSTLGCEGPSTTVTLTILPLPVITITNVATQFCEDSPIETIT
                     TITDMPGGTYSNSGQGVSGNTFNPALVTTKNTAIPINVEYTAPNTCKNNAVILFTVHH
                     LNTPIVPATITQLKDDPETILTATPDAGNSIAWYASCTSNIPLHIGNSYSTGLAGLAS
                     ADFGVRQTNIHGCRSECAPIHVDRIDCPTPAPGIANPN"
BASE COUNT          369 a          222 c          287 g          468 t
ORIGIN      
        1 atacctgtac ctgttgtggt aaaagttgga gtaggttcac cttcgcagat ttctcagtta
       61 ggatttgcta ttcccggtgc aggtgttgga caatcaatac ggtcaacatg tattggtgca
      121 cattcacttc ggcatccgtg tatatttgtt tggcgtactc caaagtctgc acttgccaat
      181 ccggctaatc cggtactata actattgcca atatgtagtg gaatatttga agtgcacgaa
      241 gcataccatg caatagaatt tccggcatct ggtgttgctg ttaatatagt ttcggggtca
      301 tctttgagtt gggtaatagt agcaggaact attggtgtat ttaaatgatg tactgtgaat
      361 aaaattacag cattattttt acaagtattt ggagctgtat attctacatt tattggtata
      421 gcagtatttt tagtagttac caatgccgga ttaaatgtgt ttccacttac accttgtcct
      481 gaattagaat atgtgcctcc gggcatatcg gtaatggtag ttattgtttc aataggtgaa
      541 tcttcacaga attgagttgc tacattcgtt atggtaataa caggtaatgg taaaattgta
      601 agagtcacgg tggtacttgg tccttcacat cctaaagtag atgttacttt ccatgtgtgt
      661 gtagaagcta ccgtgtttga tactgtaggt gtaaaagaat tgcctgtaaa tactaccgaa
      721 tttgcagcat tataccattt aatggttcct gcagttgagg tattaaacga tggtatagta
      781 ctaccactac aaatagtata gtttgcattt gttactacag gtgccggtgt tgcacaattg
      841 gtaacagtaa atgtcataca tgccggatta cttgtgcacg ttccgtcgct ttctgtcaca
      901 caataatccc acgaaccaac cgaagaactt gaaggatgtg tatatgtaga gcctgttcct
      961 attacagtat tagttgaatt tctccatgta agtgtacctc ccgaacctgt ggctgtaata
     1021 gttaaatctg atgaatttat ttcaacattt ttattgctta cagttggcgg atttatattg
     1081 actaatgtaa agtttttcgt gtctgtattg gtacaatttg ttgttgggtc tgtatattca
     1141 tacgtaacta tatggttacc tgttgaaaga cttgaagggt caatagacgt aatagttgat
     1201 ccgtctaatt ttagaatacc tccgcttggt gtagttgcag gagttatatt cgagccattt
     1261 ttacatacaa tagcaggtat tgaaacagat acagatggta aagcatttac ggttattgtt
     1321 ttcgatgcag tattggaaca accatt
//
LOCUS       DAOTOW010000693         1344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_78802_length_1344_cov_3.812258, whole genome shotgun sequence.
ACCESSION   DAOTOW010000693 DAOTOW010000000
VERSION     DAOTOW010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78802_length_1344_cov_3.812258"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="P5243_10520"
     CDS             <1..184
                     /locus_tag="P5243_10520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRS19930.1"
                     /translation="GFVWGCEFFNSAIEILANVVSPDFNEQIKQVKDISAAAVLISAI
                     VAVIVGIFVFGKYIWL"
     gene            complement(271..>1344)
                     /locus_tag="P5243_10525"
     CDS             complement(271..>1344)
                     /locus_tag="P5243_10525"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRS19931.1"
                     /translation="EITFYDKKLPIEQREKASVWEHLKRAINFTWNDRGQHGLPHLGF
                     ADWNDTVNLGYGAESLFNANLFGKAIIEMLPMCDIMGEPEYKKTLESYYAEMKKAVNE
                     HAWDGEWWKRYFKPNGEPLGSKENTYGKVFTNGQSWPAISGFAEGERLTKALDTVFEK
                     LNTAFGIKLSWPGYNGFDPSLGGVSTYPPGAKENGGIFLHANPWMMIAETIAGRGDRA
                     YQYYNQINPASKNDKLLIFESEPYCYPQNVLGDEHKQFGMGRNAWLSGTSSWTYVAGT
                     QWIVGVRPALDGLIIDPCIPTTWEKFKVTRKFRGATYKITVSNPNKISKGVKKIVVNG
                     SEIQGNKVPVFTSGEVIVEVTMG"
BASE COUNT          384 a          246 c          219 g          495 t
ORIGIN      
        1 tggttttgtg tggggttgtg aattttttaa ctctgcaatt gaaatattag caaatgttgt
       61 aagtcctgat tttaacgagc aaataaaaca agtaaaagat atttcggcag cagctgtact
      121 tatcagtgct attgttgccg tcatagttgg aatatttgtt tttggaaagt atatatggtt
      181 ataatatgta ttttttttat aatgtgaata aaaaaaggcg tttcagtaat aattgaaacg
      241 ccttttttat tttatagtga aaaaattata ttatcccata gtaacttcta ctataacttc
      301 accgcttgta aatacaggta ctttgtttcc ttgaatttca gaaccgttta caacgatttt
      361 tttaacacct tttgatattt tattggggtt agatactgtt attttatagg tagcgccacg
      421 gaattttcgt gttactttaa atttttccca tgttgtagga atacatgggt caataattaa
      481 accatctaat gcaggtctaa ctccaactat ccattgtgtt ccggctacat atgtccatga
      541 agaagttccc gataaccaag catttcgacc cataccaaat tgtttatgtt catctcctaa
      601 cacattttga ggatagcaat atggttccga ttcaaaaatc aataatttgt cattttttga
      661 tgccgggtta atctgattat aatattgata tgctctgtct ccacgtcctg caatggtttc
      721 tgctatcatc atccatgggt ttgcatgtaa gaaaatacct ccattttctt tagcacccgg
      781 aggataagta gatacgcctc ctaaacttgg atcaaaaccg ttgtaaccgg gccaacttaa
      841 tttaatacca aaagcagtat ttaatttttc aaaaacagta tcaagagcct tagttaaccg
      901 ttcgccttct gcaaatccgg aaattgcagg ccatgattgt ccgttggtaa aaacttttcc
      961 ataggtgttt tcttttgaac caagtggttc accgtttggt ttaaagtatc gtttccacca
     1021 ttcgccgtcc catgcatgtt cattaacagc ttttttcatt tctgcatagt atgactccaa
     1081 tgtttttttg tattccggtt cacccattat atcacacata ggaagcattt ctataattgc
     1141 ttttccaaat aagttagcat tgaataaaga ttctgcaccg tatcctaaat ttacagtatc
     1201 gttccagtct gcaaatccta aatgaggtaa cccgtgttga cctctatcgt tccaagtaaa
     1261 gtttattgct cgttttaaat gttcccaaac acttgctttt tcgcgttgct caataggtaa
     1321 ttttttgtca taaaatgtta tttc
//
LOCUS       DAOTOW010000694         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_79084_length_1341_cov_3.069207, whole genome shotgun sequence.
ACCESSION   DAOTOW010000694 DAOTOW010000000
VERSION     DAOTOW010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79084_length_1341_cov_3.069207"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1341)
                     /locus_tag="P5243_10530"
     CDS             complement(<1..>1341)
                     /locus_tag="P5243_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRS19932.1"
                     /translation="WETHHFATLIGYGANAVNPYMAFETITTLAEERYIAKDVDIESA
                     NKTYIYAIGAGLLKIFSKMGISTLQSYKGAQIFECYGIGKEVIESCFNGTVSKIGGLN
                     FDDIAREVLVRHKAAYPQLPIVNQRLEEGGIHQWKHRGEKHLFNPETIALLQHAVRTG
                     NKEIYKQYAKKVNDQAEQQFTLRGLLEFKGNRKPISIDEVEPASNIFPRFATGAMSFG
                     SISHEAHTTLAIAMNRIGARSNSGEGGEDEIRFVQKENGDWERSAIKQVASGRFGVTS
                     NYLANCNEIQIKMAQGAKPGEGGQLPGHKVDDWIGRVRHSTPGVGLISPPPHHDIYSI
                     EDLAQLIFDLKNANPNAEISVKLVSKAGVGTIAAGVCKAFADKVLISGHDGGTGASPI
                     SSIHHTGSPWELGLAETHQTLVLNKLRDRIIVQADGQMKTGRDLAIAALLGAEEW"
BASE COUNT          358 a          271 c          234 g          478 t
ORIGIN      
        1 ccattcttcg gctcctaaaa gagctgcgat tgcaaggtct ctaccggttt tcatttggcc
       61 atcggcttgt actataattc tatcacgtaa tttgttgagt accaatgttt ggtgtgtttc
      121 tgccaaacct aattcccatg gcgaacctgt atgatgtata gaactaatag gtgaagctcc
      181 ggtaccaccg tcgtgacccg aaattaatac tttatcggca aatgctttgc aaacacctgc
      241 agctatagtt ccaacacctg ctttcgatac cagttttaca cttatttcag cgttaggatt
      301 agcatttttt aagtcaaata ttaattgagc taaatcttca atagaataaa tatcatgatg
      361 aggtggggga gaaattaatc ctactcccgg agttgagtga cgaacacgtc caatccaatc
      421 atctacttta tgacccggta gctgtcctcc ttcacccggt tttgctcctt gagccatttt
      481 tatttgaatt tcgttacaat ttgccaaata gttactagta acaccaaaac gacccgatgc
      541 tacttgtttt attgcagaac gttcccaatc gccattttct ttttgtacaa atcgtatttc
      601 atcttcacct ccttcacccg agttagaacg agctccaatt ctattcattg ctatagctaa
      661 tgttgtatga gcttcgtgtg agatagatcc aaaagacatt gctccggttg caaaacgtgg
      721 aaatatgttg cttgctggtt ctacttcatc tatggaaata ggtttgcgat tacctttgaa
      781 ttctaataaa ccgcgaagag taaactgttg ttcggcttgg tcgtttactt ttttagcata
      841 ttgtttatat atttctttgt tgccggtgcg taccgcatgt tgtaacaatg ctatagtttc
      901 aggattaaat aagtgttttt cgcctctgtg tttccattgg tgaataccac cttcttctaa
      961 tcgttgattt actattggta attgaggata tgctgcttta tgacgaacaa gcacttcacg
     1021 tgcaatatca tcaaaattaa gtccgcctat ttttgataca gttccattaa agcatgattc
     1081 tataacttct ttgccaatac cataacattc aaatatttgt gcacctttat atgattgtaa
     1141 tgtggatata cccatttttg agaatatttt gagtaatcct gctccaattg catatatata
     1201 tgttttgttt gctgattcta tatcaacatc ttttgcaata taacgttctt ctgcaagtgt
     1261 ggtaatagtt tcaaaagcca tatatgggtt tactgcattt gctccgtaac ctataagtgt
     1321 tgcaaaatga tgagtttccc a
//
LOCUS       DAOTOW010000695         1340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_79204_length_1340_cov_2.347082, whole genome shotgun sequence.
ACCESSION   DAOTOW010000695 DAOTOW010000000
VERSION     DAOTOW010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79204_length_1340_cov_2.347082"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>493
                     /locus_tag="P5243_10535"
     CDS             171..>493
                     /locus_tag="P5243_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19933.1"
                     /translation="MPLYRNIPIFLPELACKNKCIFCNQQTISGTHSIPQPHNVAAII
                     KEHIATIPPNAHIQVAFFGGSFTGLPMATQIQYLQAVQPYISRGNVQGIRISTRPDYI
                     THDIVT"
     assembly_gap    494..503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <504..1163
                     /locus_tag="P5243_10540"
     CDS             <504..1163
                     /locus_tag="P5243_10540"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19934.1"
                     /translation="VQEIELGVQSIHNDVLLASGRGHTVEHIEQAVHIIHNHKISLGL
                     QMMIGLPGDTHAKAVETAQTIIDLGARSTRIYPTLVIAHTPLAELYKKGLYTPLSLDE
                     AVTITKDIYRLFVSNSITVLRVGLHPSKDLHNPEYVLAGPFHHAFKELVETEIWWDIF
                     EKQLPSSHKKIEIHVPHNQIQYAIGYNATNKQKLQKHFDSVNFIASNHIQEYECTYRY
                     Y"
     gene            1138..>1340
                     /locus_tag="P5243_10545"
     CDS             1138..>1340
                     /locus_tag="P5243_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19935.1"
                     /translation="MSVHTAIIDYRAPKEAILNLEHYYNICLFKNTHTYEAINGHPDI
                     CIFKGSSELIIAPNSPKILFDLC"
BASE COUNT          499 a          237 c          195 g          399 t
ORIGIN      
        1 aaacgaattc atgaacggtt aaaaatagaa ttacgagata aaattcatat tatgaaatat
       61 gaatttattg attttgaatt attattacaa tttctaatag cctggttcaa aggtcataca
      121 accgaagaag ataaacgatt aggtacattt attctacaaa aatataaata atgccgttgt
      181 atagaaacat acctattttt ttaccggaat tggcatgtaa aaacaaatgt attttttgta
      241 atcaacaaac tataagtgga acacattcaa ttccacaacc acacaatgtt gctgcaatca
      301 ttaaagaaca tattgcaaca attcccccta atgcacacat acaagtagcc ttttttggcg
      361 gtagttttac cggattgcct atggcaacac aaatacaata tttacaagct gtgcaacctt
      421 atatatcaag aggaaatgta caaggaatac gaataagtac ccgccccgat tatataacac
      481 atgatattgt tacnnnnnnn nnngtacaag aaatagaact aggcgttcaa tctattcata
      541 atgacgtgtt gttggcatct ggcagaggac acacggttga acacatagag caagcggtac
      601 atattattca taaccataaa atttcattag ggttacaaat gatgataggc ttacccggcg
      661 acacgcatgc taaagcagta gaaacagccc aaacaattat agatttaggt gctcgcagta
      721 ctcgcattta ccctacttta gttattgcac atactccatt agcagaatta tataaaaaag
      781 gattatatac tcctctttca ttagacgaag ctgttactat aaccaaagat atatatcggt
      841 tatttgtatc aaattccatt acagtgcttc gtgtaggttt acacccgtca aaagatttac
      901 acaatcccga atatgtattg gcaggcccct ttcatcatgc gtttaaagaa ttagttgaaa
      961 cagaaatttg gtgggatatt tttgaaaaac aattaccctc atctcataaa aaaatagaaa
     1021 tacatgtccc ccacaatcaa atacaatatg caataggtta taatgcaaca aataaacaaa
     1081 aattgcaaaa acactttgat tctgttaatt ttattgcatc aaatcatata caagaatatg
     1141 agtgtacata ccgctattat tgattatcgt gctccaaaag aagcaatact aaatttagaa
     1201 cattattata atatttgttt atttaaaaat actcatacat atgaagctat caacggccac
     1261 cctgacattt gtatattcaa aggcagcagc gaattaataa ttgccccaaa cagtcctaaa
     1321 atattatttg atttatgcga
//
LOCUS       DAOTOW010000696         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_79829_length_1333_cov_2.976526, whole genome shotgun sequence.
ACCESSION   DAOTOW010000696 DAOTOW010000000
VERSION     DAOTOW010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79829_length_1333_cov_2.976526"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1333
                     /locus_tag="P5243_10550"
     CDS             59..>1333
                     /locus_tag="P5243_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19936.1"
                     /translation="MIDMDVQQITWKIDNPVMIMSGLEGTTVNKASFESSNYFREARY
                     MEIGLYDKTHPLIALRNMAKEKKSETFSVADFAKYLKIPESDTRHLCLDLSFKGLIAY
                     DREEQLITVKPRVYEYLNARVGKIDYDVIRFNSEHDIKGLHNAKLDLTTFELDLVGVS
                     KVQVSDSQNVIFYPRGSQLTMSKNRNFRFDGVIDAGLFTYYGRGFDFNYEQFKINLKN
                     VDSLKIKVEAFKANQYGVKDLVEIKNAIEYITGEIMIDAPNNKSSIKKFPQYPIFDSK
                     QPSYVFYDYPAIYKGVYDRKKFYFKVDPFVIDSLNKFSTKGLFFAGEFFSADIFVPMR
                     EKIVVQNDYSFGFTRTTGAAGYPIYKGKGTFTDTINLSFKGLRGFGKINYLNSESVSK
                     TGYTFFPDSTNGIADKYSIKKQTKAQGVDYPDV"
BASE COUNT          478 a          237 c          232 g          386 t
ORIGIN      
        1 tattagaaac aacgacagtg aaaacttgtc tcgtaccccc tacttcaaca cctatcacat
       61 gatagatatg gatgtgcaac agattacttg gaaaatagac aatccggtaa tgataatgag
      121 tggtttggaa ggtactaccg taaacaaagc tagttttgaa tcatcaaact attttcgtga
      181 agcacgctac atggaaatcg gattgtatga caaaacacat ccgcttatag ccttacgtaa
      241 tatggcaaaa gagaaaaaat cagaaacatt ttcggttgct gattttgcaa aatatttaaa
      301 aattcctgaa tctgatactc gccatttatg cttggattta tcattcaaag gtttgatagc
      361 atacgaccgc gaagaacaat taattacggt aaaacctcgt gtatatgaat atttaaatgc
      421 tcgtgtaggt aaaattgact atgacgttat tcgattcaat tcagaacacg acatcaaagg
      481 attgcataat gcaaaactcg atttaacaac ctttgaacta gaccttgttg gggtgtcaaa
      541 agtacaagta agtgactcgc aaaacgtgat tttctatcct cgcggtagcc agttgaccat
      601 gtcaaaaaac agaaacttcc gattcgacgg agtcattgat gccggtcttt ttacatatta
      661 tggtagaggt tttgacttca actacgaaca attcaaaata aacctaaaaa acgttgactc
      721 acttaaaatt aaagtggagg cattcaaagc aaaccaatat ggtgtaaaag acctggttga
      781 gataaaaaat gcaatagaat atattaccgg tgaaattatg attgatgccc ccaacaataa
      841 atccagtatc aagaaatttc cgcaatatcc tatttttgac agtaagcaac cgtcgtatgt
      901 attttatgat tatcctgcta tatacaaagg tgtatacgac cgtaaaaaat tctattttaa
      961 agttgaccca tttgttattg acagtcttaa caaattttct acaaaagggt tattctttgc
     1021 cggagaattc tttagtgcgg atatttttgt gccaatgcgt gaaaaaattg tcgtacaaaa
     1081 cgattattca tttggattta ccagaactac gggcgcagca ggatacccta tttataaagg
     1141 caaaggaaca tttaccgaca ctataaacct aagtttcaaa ggattacgag gatttggtaa
     1201 aataaactac ttaaattctg agtctgtttc aaaaacagga tacacatttt ttcctgactc
     1261 taccaatggt atagcagaca aatactctat aaaaaaacaa acaaaagcac aaggagttga
     1321 ctaccccgat gta
//
LOCUS       DAOTOW010000697         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_79846_length_1333_cov_2.548513, whole genome shotgun sequence.
ACCESSION   DAOTOW010000697 DAOTOW010000000
VERSION     DAOTOW010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79846_length_1333_cov_2.548513"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="P5243_10555"
     CDS             <1..189
                     /locus_tag="P5243_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19937.1"
                     /translation="DIATESKLKHICILINDVNMINSYGYRYGAYRSNKYGYGYYEDE
                     AFEDTRLIAKIKRIVKKH"
     gene            196..>884
                     /gene="fmt"
                     /locus_tag="P5243_10560"
     CDS             196..>884
                     /gene="fmt"
                     /locus_tag="P5243_10560"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRS19938.1"
                     /translation="MRILYMGTPDFAVAPLQTLINHNYTIVGVVTAPDKPAGRGQQLS
                     MSAVKKCALQYSLPIYQPTNLKSEEFVQQVRDLQPDVILVIAFRMLPKIIWEIPPLGT
                     INIHGSLLPQYRGAAPIHWAVVHGEKITGVTSFYINEQIDTGNILLQKQIDIAYTDTT
                     GIVYEKLMQLAAELSVETLEYIIKNPHVSTPQSTINIGELRHAPKIEKETCKINWQQN
                     GEIIYNHIRGFS"
     assembly_gap    885..894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <895..1145
                     /locus_tag="P5243_10565"
     CDS             <895..1145
                     /locus_tag="P5243_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19939.1"
                     /translation="PAAWTYIIKQKNRLVFKIYFAEFIAQNHTYSPGYILIEKSVLSI
                     AVINGFIKPKDVQIEGKKRMSIEECMRGFSFDDVIIEL"
     gene            1206..1279
                     /locus_tag="P5243_10570"
     tRNA            1206..1279
                     /locus_tag="P5243_10570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1240..1242,aa:Arg,seq:tct)
BASE COUNT          496 a          181 c          238 g          408 t
ORIGIN      
        1 gatatagcta ccgaatcaaa attaaaacat atttgtattc ttataaatga tgtaaatatg
       61 ataaatagct acggatatag atacggagca tacagaagca ataaatatgg atatgggtat
      121 tatgaagatg aagcatttga agatacccga ttgattgcaa aaattaaacg aattgtaaaa
      181 aaacactaaa caagtatgag aattctgtat atgggaacac cggattttgc cgttgctccg
      241 ttacaaactc ttattaatca taattataca atagttggtg tagttacagc acccgacaaa
      301 ccggcaggta ggggacaaca attatcaatg tcggctgtaa aaaaatgtgc tttacagtat
      361 agtttaccta tttatcaacc aacgaatctt aaatcagaag aatttgtaca gcaagtacgt
      421 gatttgcaac ctgatgtaat tttagtaata gcatttagaa tgctaccaaa aataatttgg
      481 gaaataccac ctttgggtac aataaatatt catggttcat tgttgccgca atatagggga
      541 gctgctccta tacattgggc tgtggttcat ggtgaaaaaa taaccggtgt tacaagtttt
      601 tatatcaatg aacagataga tacaggtaat atattattgc aaaaacaaat agatatagca
      661 tataccgata ctaccggaat agtatatgaa aaacttatgc aattagcagc cgaattatcg
      721 gttgaaacgt tagaatatat tattaaaaat ccacatgttt caactccgca atctactatt
      781 aatattggag aattaaggca tgctccaaaa attgaaaaag aaacttgtaa aataaattgg
      841 caacaaaatg gagaaataat ttataatcat atcagaggtt tttcnnnnnn nnnntcccgg
      901 ctgcatggac atatattata aaacaaaaaa atcgattagt atttaaaata tattttgcag
      961 aatttattgc acaaaaccat acgtattcac cgggttatat attaattgaa aaatctgtgc
     1021 tttcaattgc tgtaattaat ggatttataa agcctaaaga tgtgcaaata gaaggtaaga
     1081 aacgaatgag tatagaagag tgtatgcgag gtttttcttt tgatgatgtt ataatagaat
     1141 tataaaaaaa atatgtatca ttggtattgt agagtaaaaa ttttatattt gcagcaaaat
     1201 taaacggtct ggtagctcag ctggatagag caacagattt ctaatctgtg ggtcgtgggt
     1261 tcgaatcccg cccggatcac ataaagcctc aattttgatt gaggcttttt tgtttttgta
     1321 atgttaaaat gca
//
LOCUS       DAOTOW010000698         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_80071_length_1330_cov_4.039216, whole genome shotgun sequence.
ACCESSION   DAOTOW010000698 DAOTOW010000000
VERSION     DAOTOW010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80071_length_1330_cov_4.039216"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..>1330)
                     /locus_tag="P5243_10575"
     CDS             complement(47..>1330)
                     /locus_tag="P5243_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19940.1"
                     /translation="EDITSPYSYTWTNVANGTYNITAKAFDDKNASTTSQSITVIVIN
                     ATTPTLQGTNLNQTVIKQTAITPMIFTWGNAATDVTYSSLPTGLIAVKNSSAKTLTVS
                     GTPSVNGTFTVTTIGGSPAVSLQASVTLNPNSILANWYPFQENPISLSFISFANASLN
                     PSQDNSAYSSIGCTTGALTLAKGTGELQIEVQSLQSLQIRWYATGGRTLQVVYGPTGT
                     ENVWNSPSQYSSGGYQHNLTSMIPALITNQPIIVRIINNRTDGGSLHITDLYVEGSVP
                     KVSQVTQTISLQQGWNLISLNIRPADTTIATLFAGKDVLVVKDAQSFWVKNNTSQLQS
                     LQKLTPGIGYLVKMSTATTLTIQGVPLNQTITIPNNPGWYLIGCPYQTVTPIYNYFNI
                     NNCKQIKNFTGFWIPDGTTNSITNFEPGKGYFLKK"
BASE COUNT          421 a          217 c          234 g          458 t
ORIGIN      
        1 atttgcatgg tatacacaac aagactaata ataagcaggc ttagtgctac ttcttaagaa
       61 aataaccttt gcccggttca aagttagtta tagaatttgt agttccatcg ggaatccaaa
      121 atcctgtaaa atttttaatt tgcttacaat tatttatatt aaaataattg taaataggag
      181 taactgtttg atatggacaa cctattaaat accatcccgg attattcggt attgtaatag
      241 tttgattcaa aggaactcct tgtatagtaa gagtggttgc tgtactcatt tttactaaat
      301 atcctattcc cggtgtcagt ttttgtaaag actgcaattg agatgtatta tttttaaccc
      361 aaaatgactg cgcatctttt acaactaaaa catctttacc tgcaaataac gttgctatag
      421 tagtatctgc aggccttata ttaagtgata taagattcca accttgttgt aatgaaattg
      481 tttgagttac ttgagatact tttggaacag aaccttctac atacaaatct gtaatatgca
      541 aactacctcc atctgttcta ttattaatta ttctaacaat aattggttgg tttgttatta
      601 atgcgggtat catagatgtt agattgtgct gatagccccc actggaatat tgcgaaggtg
      661 aattccaaac attttctgta ccggtaggac catataccac ctgtaaagtt ctacctcctg
      721 tagcatacca acgtatttgc aacgactgca acgactgaac ttctatttgc aattcacctg
      781 taccttttgc aagagttaaa gcaccggtgg tacatccaat agatgaatat gcactattat
      841 cttgtgaagg attaagcgag gcatttgcaa aagaaataaa agataaactg ataggatttt
      901 cttgaaacgg ataccaatta gctagtattg aattaggatt taaagtaaca cttgcttgaa
      961 gagaaactgc gggactacct cctatagtag ttacagtaaa agttccattt acagaagggg
     1021 tacctgatac tgtaagagtt tttgcagaag aattttttac agcaataagt ccggttggca
     1081 aagaagaata tgttacatct gtagctgcgt taccccatgt aaatatcatt ggtgtaatag
     1141 ctgtttgctt tatcactgtt tgattcaaat ttgttccttg taaagtagga gttgttgcat
     1201 ttattacaat tacagtaata ctttgtgaag tagttgaagc atttttatca tcaaaagctt
     1261 ttgctgttat gttataagta ccatttgcaa catttgtcca cgtatatgaa tacgggcttg
     1321 taatatcttc
//
LOCUS       DAOTOW010000699         1329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_80175_length_1329_cov_3.843014, whole genome shotgun sequence.
ACCESSION   DAOTOW010000699 DAOTOW010000000
VERSION     DAOTOW010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80175_length_1329_cov_3.843014"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1329)
                     /locus_tag="P5243_10580"
     CDS             complement(<1..>1329)
                     /locus_tag="P5243_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS19941.1"
                     /translation="LTRFAIVYILLAVFGTMVVAKIVHIQFVEGAELKQMNKKQQTVV
                     VKPKRGDIYSRNGKVLACSVPEYVVYFDYRIPAFKKSDTLFKNNVNALAEGLAKLFPE
                     EGKTKKQYLEALYKTAKSYSYLRLHKKPIDYNKLQEVKKLPILREGSFKGGLLIDVYN
                     SRVYPYGDIARRTIGLLRKGSWHGETGLEMYYDSYLYGIEKTEKTEDDPAMVNKDGVV
                     DGYDIITTIDTEIQSIANEELLKALQQYKAVWGCAVVMEVKTGEVLAISNLSRTGNPA
                     DSNYYELENFAVNFRGDPGSTIKLPSLMIALEDGVIDLNDTIDTGGGHIRYVNKGIET
                     NVYDWNFKTQGGFHKLSVKGVFANSSNVGVSKIIYNNYVATRKEWDYINRLKSMGFGK
                     PSGIDLINERIPLVKDPSMTTKNDVQKWEGSTLIQMSYGYDIEVTPLQML"
BASE COUNT          394 a          271 c          171 g          493 t
ORIGIN      
        1 taagcatttg caatggtgta acttcaatat cgtacccgta tgacatttga ataagtgtac
       61 taccttccca tttttgtaca tcatttttgg tagtcatact tggatctttt acaagtggaa
      121 ttcgttcatt tattaaatct atcccacttg gtttgccaaa ccccatactt ttcaaacgat
      181 ttatatagtc ccattctttg cgagtagcta cataattgtt atatattatt ttactaaccc
      241 ctacattcga tgaatttgca aacactcctt ttactgaaag tttatgaaat ccgccttgtg
      301 ttttaaaatt ccaatcatac acattggttt ctataccttt atttacatat cttatatgcc
      361 cccctccggt gtctattgta tcatttaaat ctattacacc atcttctaag gcaatcataa
      421 gtgatggtag tttaatagtt gaacccgggt cgcctctaaa atttacagca aaattttcta
      481 actcataata atttgaatca gcaggattac cggtacgaga aagatttgaa atagccaaaa
      541 cttcaccggt tttaacttcc attactacag cacaccccca aacagcctta tattgttgta
      601 aggctttaag taactcttca tttgctatac tttgaatttc ggtatctatt gttgtaatta
      661 tatcataacc atcaaccaca ccatctttat ttaccattgc agggtcatct tcggttttct
      721 cggttttttc tattccatat aaatacgaat cgtaatacat ttcaagaccg gtttctccat
      781 gccaactgcc tttgcgcagc aatcctatag tacgacgagc aatatcgcca tagggatata
      841 cacgactgtt atacacatca ataagtaatc cccctttaaa tgaaccttct cttagaattg
      901 gtaatttttt tacttcttgt aatttattat aatcaatagg ttttttatgt aaacgtaagt
      961 atgaatatga ttttgcagtt ttatacaatg cttctaaata ttgtttttta gtttttcctt
     1021 cttcaggaaa caatttagca agcccttctg ctaatgcatt aacattattt ttaaacaatg
     1081 tatcactctt cttaaatgca ggtattctat aatcaaaata caccacatat tcgggaaccg
     1141 aacaagcaag tacttttcca tttctagagt atatatcacc acgttttggt tttactacta
     1201 ccgtttgctg ctttttgttc atttgtttca attcggcacc ttctacaaat tgaatatgaa
     1261 ctatttttgc aaccaccatg gttccaaaaa cagcaagtag tatatacact attgcaaacc
     1321 ttgttaata
//
LOCUS       DAOTOW010000700         1324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_80709_length_1324_cov_2.349882, whole genome shotgun sequence.
ACCESSION   DAOTOW010000700 DAOTOW010000000
VERSION     DAOTOW010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80709_length_1324_cov_2.349882"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>832)
                     /locus_tag="P5243_10585"
     CDS             complement(<1..>832)
                     /locus_tag="P5243_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19942.1"
                     /translation="PNDYIKWNITSKETTQKNAQLNQSTFNPDEFLVKNDEITKPWVY
                     TIKGTYYVKAQSGDICESNPAEMTYTVYPRARKPIVAATTICQGTKIEPIQAFGSPLI
                     EWFPQHPSIQDKKGYGQTYNFEMLGITDLDTGLYEFKLRDKTILKNIPDIEDEGCYSN
                     EIIATLRVAPAAKTKIIGDSTLCEYTMEEIYTLAYSPEKPSKYRWEVTGNNIAYTKDA
                     NSTAVRYVDWSKAGIDTIKVYERTYYECDGYDELIVNIAEYPKAKYTWSLPGASNEVW
                     "
     assembly_gap    833..842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<843..>1324)
                     /locus_tag="P5243_10590"
     CDS             complement(<843..>1324)
                     /locus_tag="P5243_10590"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19943.1"
                     /translation="QIQWYDEAKLPLTTGSTYQYIHTSAGLKTYYATQTIQGCTSPEA
                     KVTFTVFAKPNKPETTGAEICEGKDPVTLRAQVSQDVWYFDNNASQPTGIKGPLYLPQ
                     GTLNKTTSFYVRREQNGCVSDIAEVQLKVIPKPTIVITQPNRAKCVYDETNIIQTNLT
                     "
BASE COUNT          366 a          213 c          228 g          507 t
ORIGIN      
        1 aaccatactt cattagaagc tccaggcaat gaccatgtat atttagcttt aggatattca
       61 gcaatattta ctattaattc gtcataccca tcacattcat agtatgtgcg ttcatatact
      121 tttatagtgt caatacctgc ttttgaccaa tctacatatc gtacggcagt tgaattagca
      181 tctttggtat atgctatgtt attaccggta acttcccaac ggtatttact tggtttttca
      241 ggtgaatatg ctaaggtata tatttcttcc atggtatatt cacacagtgt agaatctcca
      301 ataatttttg tttttgctgc aggagctact cgtaatgtag caattatttc attgctgtag
      361 catccttcat cttcaatatc gggaatgttt ttaagtatag ttttatctcg taatttaaat
      421 tcatataaac cggtatctaa atcggtaatg cccaacattt caaaattata agtttgtcca
      481 tatccttttt tatcttgtat acttgggtgc tgaggaaacc attcgataag tggactgcca
      541 aatgcttgta taggctcaat tttggtgcct tgacatatag ttgtagctgc aactataggt
      601 ttacgtgcac gtggatatac agtgtatgtc atttctgccg gattactttc acaaatatct
      661 cctgattgag cttttacata ataggtgcct ttaatagtat atacccacgg tttagtaatt
      721 tcatcatttt ttactaaaaa ttcatcagga ttaaaagtag attgatttaa ttgtgcattt
      781 ttttgagtcg tttcttttga agttatattc catttaatat aatcatttgg tcnnnnnnnn
      841 nngtgttaga ttagtttgta taatgttggt ttcatcataa acacattttg cacgattagg
      901 ttgagtaatt acaatggtag gtttaggaat tacttttaat tgaacttctg caatatcact
      961 tacacaaccg ttttgttcgc gacgaacata gaatgatgta gttttattca acgtaccttg
     1021 cggtaaatat aatggacctt taataccggt tggttgtgaa gcattgttat caaaatacca
     1081 tacatcttga cttacttgtg ctcgtaaagt tacaggatct tttccttcac aaatttcggc
     1141 accggtggtt tccggtttat taggtttagc aaatacagta aatgttactt tagcttcggg
     1201 actggtacat ccttgaattg tttgtgttgc ataataggtt ttaagtcctg cagaggtatg
     1261 aatatattga tatgtagaac ctgtagttaa tggtaatttt gcttcatcat accattgaat
     1321 ttgt
//
LOCUS       DAOTOW010000701         1322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_80878_length_1322_cov_2.621152, whole genome shotgun sequence.
ACCESSION   DAOTOW010000701 DAOTOW010000000
VERSION     DAOTOW010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80878_length_1322_cov_2.621152"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..852
                     /locus_tag="P5243_10595"
     CDS             4..852
                     /locus_tag="P5243_10595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS19944.1"
                     /translation="MVKIKRKRYYISYFFISVCCLFLFSFDGIKDKHKKAVIQTNYGT
                     ITIQLYNQTPLHRDNFIKLTESHFYDSLLFHRVIPNFMIQCGDPLSKGANNKQFLGNG
                     GPGYTIPAEFVPGLIHKKGAIAAARLGDEQNPEQASSGSQFYIVQGKVCSLQELQQIE
                     EQRITKMTYKVIRDFLQKPENDALRNQIIALQKDGKTTEYNALIEEIKAKLPQEMARI
                     ESFRYTDEQKKIYTTIGGTPHLDYEYTVFGEVIDGFDVVEKISHVETKYDRPRINVVM
                     KISMIK"
     gene            861..>1322
                     /locus_tag="P5243_10600"
     CDS             861..>1322
                     /locus_tag="P5243_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRS19945.1"
                     /translation="MLQKISQLLEIVKGFEAHNKEQVEQFRLQYLSKKGELSQLFDMF
                     KDVPNDQKKEFGKQLNILKQVIQEKYDFLQSQFQSTEDVLQGIDITLPENPIRLGSNH
                     PLSIVKNRIIEIFSKIGFVVSEGPEIEDDWHVFTALNFPLEHPARDMQDTFF"
BASE COUNT          511 a          173 c          214 g          424 t
ORIGIN      
        1 tgtatggtaa aaataaaaag aaagagatat tatatatcgt atttttttat tagcgtttgt
       61 tgtttgtttt tattttcgtt tgatggtata aaagataaac ataaaaaagc tgttattcaa
      121 actaattatg gtacaataac aattcagtta tataatcaaa cgccattgca tcgtgataat
      181 tttataaaac ttacagagtc acacttttat gatagtttgt tgtttcatcg tgtaatacca
      241 aattttatga ttcaatgtgg agacccttta tcaaaaggtg caaacaataa acaattttta
      301 ggtaatggtg gaccgggata tactatacct gctgaatttg tgccgggtct tattcataaa
      361 aaaggagcta ttgctgcagc acgcttaggt gatgagcaaa atcctgaaca agcatcttca
      421 ggctctcaat tttatatagt tcaaggtaaa gtatgtagtt tgcaagaatt acaacaaata
      481 gaagaacaaa gaattactaa aatgacatat aaggtaattc gagatttttt acaaaagcct
      541 gaaaatgatg ctttacgtaa tcaaattatt gcattgcaaa aagatggaaa aaccaccgaa
      601 tataatgctc tcattgaaga aattaaagct aaattgccac aagaaatggc taggatagaa
      661 tcttttagat atacagatga acaaaaaaaa atatatacta caataggcgg tactcctcat
      721 ttagattatg aatacaccgt atttggcgag gttatagatg gatttgatgt ggtagaaaaa
      781 atttcgcacg ttgaaactaa atatgatagg ccaagaataa atgtagtaat gaaaatttca
      841 atgattaaat aatatacaat atgttacaaa aaatttcaca acttttagaa attgtaaaag
      901 ggtttgaagc tcataataaa gaacaagttg aacaatttcg tttacaatat ttatcaaaaa
      961 aaggtgaatt atcacagttg tttgacatgt ttaaagatgt acctaatgac caaaaaaaag
     1021 aatttggaaa acaacttaat attctaaaac aagttattca agaaaaatat gattttttgc
     1081 aatctcaatt tcaatcaacc gaagatgtgt tgcaaggtat agatataacc ttacctgaaa
     1141 atcctatacg tttagggagt aatcatccac tttctatagt taaaaataga ataatagaaa
     1201 tattctcaaa aattggattt gttgtatctg aaggacccga aatagaagat gattggcatg
     1261 tgtttactgc ccttaatttt cctttagaac atcctgctcg cgatatgcaa gatacatttt
     1321 tt
//
LOCUS       DAOTOW010000702         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_81026_length_1320_cov_4.052174, whole genome shotgun sequence.
ACCESSION   DAOTOW010000702 DAOTOW010000000
VERSION     DAOTOW010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81026_length_1320_cov_4.052174"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..662)
                     /locus_tag="P5243_10605"
     CDS             complement(9..662)
                     /locus_tag="P5243_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRS19946.1"
                     /translation="MRTIIICYILICTSLAGFSQKTSVNIGVLKYGGGGDWYANPTSV
                     PNLIQFCNKQLPATIASSSVVDIGSPELYNYTMVHMTGHGNVLFSSTDVQNLRNYLIS
                     GGFLHIDDNYGMDQYVRREMKKVFPELDFIELSPQHPIYSQAFPFPKGLPKIHEHDGK
                     PAQGFGLFYEGRLICFYTFECDLGDGWEDKEVHNDSDETRTKALKMGANIVQYICTH"
     gene            848..920
                     /locus_tag="P5243_10610"
     tRNA            848..920
                     /locus_tag="P5243_10610"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:881..883,aa:Met,seq:cat)
     gene            936..>1320
                     /locus_tag="P5243_10615"
     CDS             936..>1320
                     /locus_tag="P5243_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRS19947.1"
                     /translation="MRENSIFKKRILQYLEFKNITKYEFYQKTGISNGILSQTNGLTE
                     DSILKFLSYYTDVNPSWLLTGGGEMITNNQTKEYTNTVNEPKSTYNTVDKKQIITAGD
                     LLDRIEKQSEEIGNLKAQIQQLTSKK"
BASE COUNT          473 a          212 c          234 g          401 t
ORIGIN      
        1 acattagact aatgtgtgca tatgtattgt acaatgtttg ctcccatttt aagagcttta
       61 gtgcgggttt catcgctatc gttatgaact tctttatctt cccaaccatc acctaaatcg
      121 cattcgaagg tgtagaaaca aataagtctt ccttcgtaga atagtccaaa accttgagca
      181 ggtttgccat cgtgttcatg tattttgggt aatcctttgg gaaatgggaa cgcttgtgaa
      241 taaataggat gttgaggtga aagttctata aaatcgagtt cgggaaatac ttttttcatt
      301 tcgcgtctta catattgatc catgccataa ttatcatcaa tatgtaaaaa acctccactt
      361 ataagataat ttcgtaaatt ttgaacatcg gtagaagaga atagtacatt accgtgacca
      421 gtcatgtgta ccatagtata attatataat tcagggctgc caatatcaac tacactcgaa
      481 gaggcaatag ttgcgggtaa ttgtttgttg caaaattgta tgaggtttgg taccgatgta
      541 ggatttgcgt accagtcgcc accaccgccg tatttgagta caccaatatt tacacttgtt
      601 ttttgagaaa atcctgcaag tgaagtgcat attagtatat agcatattat tattgttcgc
      661 atagtatcaa tatttagtat acaaatgtaa tataaagtca tgtatttcaa agtatttaaa
      721 attaaattca taaaaaagta aaatatattt gacatgtgaa aataaatact atatatttgc
      781 agcctcaaaa ataagatatc atgagagcat gataaatcaa aaaattgaaa tagatataaa
      841 aatatatcgc gggatggagc agttggtagc tcgttgggct cataacccaa aggccgtcgg
      901 ttcgagtccg gctcccgcaa ctaccgaggc tatctatgag agaaaattca atttttaaga
      961 aaagaatttt gcaatatctt gaatttaaga atattacaaa gtatgagttt tatcaaaaaa
     1021 ctggtatatc aaatggtata ttatctcaaa ctaacggctt gactgaagat tcgatattga
     1081 aatttctctc atactacaca gatgttaatc cgtcttggtt attaactggt ggtggtgaaa
     1141 tgattacaaa caatcaaaca aaagaatata caaatacggt aaatgaacct aaatcaacat
     1201 ataatacggt tgacaagaaa caaataataa cagcaggtga cctgttggac cgtatagaaa
     1261 aacagagcga agaaattggc aacctcaaag ctcaaataca acaactcaca agcaaaaaat
//
LOCUS       DAOTOW010000703         1318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_81200_length_1318_cov_3.821061, whole genome shotgun sequence.
ACCESSION   DAOTOW010000703 DAOTOW010000000
VERSION     DAOTOW010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81200_length_1318_cov_3.821061"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="P5243_10620"
     CDS             <1..644
                     /locus_tag="P5243_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HRS19948.1"
                     /translation="LCSDHVNDLLTVDQKFAEQYYNYTTGKISNLYGFEVYQYEDSPY
                     YTVSTKAKLPFASVPGDTDRQASVAFFAPRMMRATGTTQAYLSEAKNNPETQENLVNF
                     RHMFICLPLKEEAIGAIVSDFAATENESITVTPTTMSFAAAGESKLAAITASSAFTAE
                     VTGTGFTINLVGNAITVTAAANAGAQRTGTVTITVTADPTKTAIIELTQLTGE"
     gene            707..1165
                     /locus_tag="P5243_10625"
     CDS             707..1165
                     /locus_tag="P5243_10625"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRS19949.1"
                     /translation="MNTIQYLVIHCTATPEGRNVTSADIRAWHTTPKPKGRGWKQVGY
                     TDMFHLDGRVERLVSNNEDAKVDAWEITNGVAGINSVSRHIVYVGGVAADGKTPKDTR
                     TWQQKRALEKYIKEFRAKHPGVKIVGHNYFDTHKACPSFNVDVWLKTIDL"
BASE COUNT          467 a          232 c          273 g          346 t
ORIGIN      
        1 tattgtgttc tgaccatgtt aatgatttgt tgacagtaga tcaaaaattt gcagaacaat
       61 actataatta tactaccgga aaaatatcga acctatatgg ttttgaggta tatcaatacg
      121 aagattctcc ttactacacg gtaagtacaa aagctaaatt accatttgct agtgttcccg
      181 gtgatacaga cagacaagca tcggtagcat tttttgcgcc acgaatgatg cgtgctactg
      241 gtactacaca ggcttaccta agcgaagcga agaacaatcc ggaaacgcaa gaaaaccttg
      301 taaacttccg ccatatgttt atatgcttgc ctcttaaaga agaagctatt ggtgctatag
      361 taagtgattt tgcagctaca gaaaacgaaa gcataacagt aactcctaca actatgagtt
      421 ttgctgcagc aggtgaatct aaattagcag caattactgc atcgagtgcg tttacagcag
      481 aagtaacagg tacaggattt acaattaact tggttggtaa tgctattacc gtaacagcag
      541 ctgctaatgc aggagcgcaa cgaacaggaa ctgtaacaat aacagtaact gctgatccta
      601 ccaaaacagc aattattgaa cttactcaat taacaggtga ataatagata atacatacaa
      661 aaaagcaagg gtgtaacgcc cttgcttttt tctaacaata aaaaccatga atactataca
      721 atatcttgta atacattgca cagcaacgcc cgaaggtaga aatgtaacat ctgccgacat
      781 acgtgcgtgg cataccaccc caaaaccaaa aggcagagga tggaagcagg ttggttatac
      841 agatatgttt catttggatg gtcgcgtaga acgattggtt tctaacaatg aagatgctaa
      901 ggtagatgca tgggaaataa caaacggtgt agccggtatt aatagtgtga gtagacatat
      961 agtatatgtt ggtggtgttg ccgctgatgg aaaaacacca aaagatactc gtacatggca
     1021 gcaaaaaaga gcccttgaaa agtatataaa agaatttaga gcaaaacatc cgggagtaaa
     1081 aattgttggt cacaattatt ttgacacaca taaagcatgt ccatcattta atgtggatgt
     1141 ttggcttaaa acaattgatt tgtaatggat actgcaaata taataatagc cgtagttggt
     1201 tcactgtttg gcagtggtgt tacctggata tttacactac gaagtgtgaa acaaaaaagt
     1261 tccgcagagg cagagctaac agctaatgaa gcaaaatcga aggagcttga caatatac
//
LOCUS       DAOTOW010000704         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_81388_length_1316_cov_6.042823, whole genome shotgun sequence.
ACCESSION   DAOTOW010000704 DAOTOW010000000
VERSION     DAOTOW010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81388_length_1316_cov_6.042823"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="P5243_10630"
     CDS             complement(<1..585)
                     /locus_tag="P5243_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRS19950.1"
                     /translation="MKYYKIVNTEGHNGLIYHEGINIDPLPFNPSGDCEPGGIYFAKE
                     DILAFVNYGTELYEVEPIGDVYENPGKPKKYKAHEVNMKYIGKVIDNIEMLIKEGANI
                     HIDDDFVLRWAAENGHHNIIKLLIKNGVDIHIYNDAALRWAAYNGHYDIVRLLLKHGA
                     NVHADNDAALRWAVENEHYKIVKLLLDNGANVHAN"
     gene            complement(620..793)
                     /locus_tag="P5243_10635"
     CDS             complement(620..793)
                     /locus_tag="P5243_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19951.1"
                     /translation="MEEKVEYCVVLEEKTIRFIGKTNTLLEAKKIKEKRKLQPNQKLW
                     IGKYVNGVLQESI"
     gene            complement(813..1166)
                     /locus_tag="P5243_10640"
     CDS             complement(813..1166)
                     /locus_tag="P5243_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19952.1"
                     /translation="MENIKILRELTEKFYRDNVSKMIMPTLKSMHDKSRTIYNDNNGE
                     IGYRRLNIMNTLANELTSTIRNYTRPDKDLGRFNREYSGIYIVPYSWLKRQLSIYNYN
                     DNILNDYMSIVDDCF"
BASE COUNT          385 a          241 c          146 g          544 t
ORIGIN      
        1 attcgcatga acattcgcac cgttatcaag taatagcttc acaattttat aatgttcatt
       61 ttctactgcc caacgtaatg cagcatcatt atctgcatgt acgttagcac catgtttcag
      121 taataatctt acaatatcat aatgtccatt gtatgctgcc caacgtaatg cagcatcatt
      181 atatatatga atatcaactc cgtttttaat caacaatttg ataatattat gatgaccatt
      241 ttctgctgcc caacgtaata cgaaatcatc atctatatgg atattagctc cttccttaat
      301 taacatctct atattatcaa ttaccttccc aatatatttc atgttgacct catgtgcctt
      361 gtatttcttt ggtttacccg gattctcata tacatcaccg ataggctcaa cttcatacaa
      421 ttcagtgcca taatttacaa atgcaagaat atcttctttt gcgaagtata tccctcccgg
      481 ttcgcaatca ccgctcggat tgaatggtag tgggtcaatg tttatgccct catggtatat
      541 taaaccatta tgaccttcgg tgtttactat tttataatat ttcataattt gttatttttt
      601 ttagttaaac atagttaatc taaattgatt cctgtaaaac accgtttacg tattttccaa
      661 tccatagttt ttgatttggt tgtagtttac gtttctcttt tatctttttc gcttctaata
      721 aagtgttagt ttttccaatg aatctaattg ttttttcttc taacactaca caatattcta
      781 ctttttcttc catagttata gttaataaat tattaaaaac aatcatctac aattgacata
      841 taatcattta atatattatc attgtagtta taaattgata attgtctttt tagccatgaa
      901 tatggtacta tatatatacc actatattcc cgattaaaac gacccaaatc tttatccgga
      961 cgtgtataat ttctaatggt gcttgttaat tcatttgcta atgtattcat aatgttcaat
     1021 cgcctatatc caatttcacc attgttatca ttatatattg ttcggctttt atcgtgcata
     1081 gattttaatg ttggcattat cattttgctt acattatcac gatagaattt ctctgttaat
     1141 tcacgaagaa ttttaatatt ttccataatc cttttgtttt atttatttgt taatataact
     1201 ttctaataac tttactatct ttttatgatc attatatctt gcccaactta atgcttcttc
     1261 attatttgca tgaatattcg cactatgttc aattaataat ctcacaattt cataat
//
LOCUS       DAOTOW010000705         1315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_81487_length_1315_cov_3.903175, whole genome shotgun sequence.
ACCESSION   DAOTOW010000705 DAOTOW010000000
VERSION     DAOTOW010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81487_length_1315_cov_3.903175"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..>1315
                     /locus_tag="P5243_10645"
     CDS             168..>1315
                     /locus_tag="P5243_10645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS19953.1"
                     /translation="MELFENSNLLPEVQQAIKEMGFERPTEIQSQAIPFLLANKRDLI
                     ALAQTGTGKTAAFGLPIISKIDLSNSTPQALIICPTRELCLQIERDLKNFGKYLPLNT
                     LAVYGGASINDQLTWLRRGVHIIVATPGRLVDIINRGKVNLSQVSYLVLDEADEMLNM
                     GFQEDIDTILEQTPENKQTLLFSATMPQEVRRIANNYMINPEEITIGTKNSGAETVSH
                     HAYIVHSKDRYIALKRLADYYPDIYGIVFCRTRQETKDIADKLMSDGYNADALHGDLS
                     QAQRDYVMKKFRNKNLQILVATDVAARGLDVQNLSHVINYSIPDDIETYTHRSGRTGR
                     AGKEGISIAILNLKEKGKLKQIERLIKKTFEIKQVPGGKEICNIKILD"
BASE COUNT          486 a          226 c          222 g          381 t
ORIGIN      
        1 ttgcggcact ttcataagag aaagttttaa aaggcattac atcaaacaaa aaatatccgg
       61 catatttttt cgcataattt cacttattgc atttgtaatt cctttaccgt tagattttgc
      121 aggtaactct gcgatatata agtaataaat taacaaatca atattgtatg gaattatttg
      181 aaaatagcaa tttgttgcca gaagtgcaac aagctattaa agaaatggga tttgaacgcc
      241 caacagaaat tcaaagtcaa gctatccctt ttttgcttgc aaacaaacgc gatttaattg
      301 cgttagcaca aacaggtacc ggaaaaacag cagcattcgg actaccaatt atttcaaaaa
      361 tagacctgag taattctact ccacaggctc ttattatttg tcctactcgt gaactttgtt
      421 tacaaataga acgtgattta aaaaactttg gaaaatattt gccactcaac acattagctg
      481 tatatggagg agcaagtatc aacgaccaat taacgtggct ccgtcgtgga gttcatataa
      541 ttgtagctac ccctggacga ttagtagata taattaacag aggcaaagta aatctctcac
      601 aggtttcata tttagttctt gatgaagcag acgaaatgct gaacatgggt tttcaagaag
      661 atatagacac catattggaa caaactcctg aaaacaaaca aaccttacta ttttcggcta
      721 ctatgccaca agaagtaaga agaattgcaa acaattacat gataaacccc gaagaaatta
      781 ccataggtac aaaaaattcg ggagccgaaa ctgtttcgca tcatgcatat atagttcatt
      841 ccaaagatcg ctatatagct cttaaacgat tagctgatta ctatcctgat atatatggta
      901 ttgttttctg cagaactcgt caagaaacaa aagatattgc agataaatta atgagcgatg
      961 gttataatgc cgatgcttta catggcgatt tatcgcaagc ccaacgcgat tatgtaatga
     1021 aaaaatttcg gaataaaaat ctccaaatat tagtagctac agacgttgct gctcgtgggc
     1081 ttgatgtaca aaatttatct catgtaatta attattcaat acctgacgat attgaaacat
     1141 atacacaccg aagcggtaga accggtagag caggtaaaga aggtatatca attgctattt
     1201 taaacctaaa agaaaaaggt aaattaaaac aaatagaacg ccttattaaa aaaacatttg
     1261 aaattaaaca agtgcccggt ggtaaagaaa tttgtaacat taaaattctt gattt
//
LOCUS       DAOTOW010000706         1310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_82037_length_1310_cov_2.198406, whole genome shotgun sequence.
ACCESSION   DAOTOW010000706 DAOTOW010000000
VERSION     DAOTOW010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82037_length_1310_cov_2.198406"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /gene="nadC"
                     /locus_tag="P5243_10650"
     CDS             <1..669
                     /gene="nadC"
                     /locus_tag="P5243_10650"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRS19954.1"
                     /translation="TFDSSLSINVLIEDGTPITKGDVVFTVHGKTISILQTERLVLNF
                     MQRLSGIATQTHEYVKLLEGTHTKILDTRKTTPGMRLLEKYAVKTGGGENHRIGLYDM
                     ILIKDNHIDYAGGVKAAIAKTKEYLLQNNLDLQIVVEARTMQDIADILTFTDIYRILI
                     DNFTPEQTKEAIAFISGRCKTESSGGITKESIRAYAEAGVDYISVGALTHQIRSLDLS
                     LKAF"
     gene            679..>1310
                     /locus_tag="P5243_10655"
     CDS             679..>1310
                     /locus_tag="P5243_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRS19955.1"
                     /translation="MKHISYILRIVLIFSFFLTFASSYSQVQKPKLIVQIVVEQMRYD
                     YITRFMPHFSKGGFKKLLYQGVQCQNTHTNYAYTQSASGIASISTGTTPSHHGIVGNT
                     WYNRNTQSKVYSVYDATKQCVGCKQPNAIMASAQELLATTFADELYTSSKGLSKIFSV
                     SLEAQSAILMAGHRPTGVYWLDEYTGNWVTSSYYAQQLPAWLQSFNNKRFA"
BASE COUNT          457 a          200 c          239 g          414 t
ORIGIN      
        1 acgtttgatt cctctctttc aattaatgtg ttaattgaag acggaactcc aattacaaaa
       61 ggtgatgttg tttttacagt tcatggtaaa acaatttcta ttttacaaac agaacgattg
      121 gttttaaatt tcatgcaacg actgagtggt attgccacac aaactcatga atatgtaaaa
      181 ctgttagaag gtacacatac aaaaattctt gatactcgta aaaccacacc cggcatgcga
      241 ttattagaaa aatatgccgt aaaaacagga gggggcgaaa atcatagaat aggattatat
      301 gatatgattt taataaaaga taatcatata gattatgcgg ggggtgtaaa agcagcaatt
      361 gctaaaacaa aagaatattt attacaaaat aatttagatt tgcaaatagt tgtagaagct
      421 cgtacaatgc aagatatagc tgatattctt acatttactg atatttatag aattttaata
      481 gataacttta ctcccgaaca aaccaaagaa gctatagctt ttattagtgg acgatgtaaa
      541 actgaatcat cgggaggaat tacaaaagaa agcatacgtg cctatgcaga agctggtgtt
      601 gattacatat ctgtaggtgc attaactcat caaattcgta gtttggattt aagtcttaag
      661 gcattttagc agttatatat gaaacacata tcgtatatat tgcgaatagt tttgattttt
      721 agtttttttt taacattcgc atcttcgtat tctcaagttc aaaaaccaaa attaattgtt
      781 caaattgttg ttgaacaaat gcggtatgat tatattacgc gatttatgcc tcattttagt
      841 aaaggtggtt ttaaaaagct tttgtaccaa ggagtgcaat gtcaaaatac acatacaaat
      901 tatgcatata cgcaaagtgc cagcggaata gcatctattt caacaggaac tacaccttcg
      961 caccatggaa tagtgggaaa tacatggtac aatagaaata cccaaagtaa agtgtatagt
     1021 gtgtatgatg caacaaaaca gtgtgttggt tgcaaacaac caaatgcaat tatggcttct
     1081 gctcaagaat tacttgcgac tacgtttgct gacgaattat atacaagtag taaagggctt
     1141 tctaaaattt ttagtgtttc gcttgaagca caatcagcta ttcttatggc aggacataga
     1201 cctacgggag tttattggct cgatgaatat accggcaatt gggtaacaag ttcttattat
     1261 gctcaacaat tgcctgcctg gcttcaatct tttaataata agcggtttgc
//
LOCUS       DAOTOW010000707         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_82393_length_1306_cov_3.005596, whole genome shotgun sequence.
ACCESSION   DAOTOW010000707 DAOTOW010000000
VERSION     DAOTOW010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82393_length_1306_cov_3.005596"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..>1306)
                     /locus_tag="P5243_10660"
     CDS             complement(86..>1306)
                     /locus_tag="P5243_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS19956.1"
                     /translation="HLYHRGGYLKNGDEVLYFVSNRPGGKGGYDIWFSIYNPRKKLWR
                     EPKNCGNKINSIGDELTPFYDNKTRTLYFSSTGWPGLGELDIFSSIGEMGKFNPAENI
                     GYPINSPFDDLYYVLHHDGKKGFFTSNRPGTVSYSHPTCCDDLFSFNYLGVINVAVTG
                     KVFEVYNKKIHDILKSQIETKNTIAIDTIQKKYIEGSIVTLYLHDKTNKERTLLQKDS
                     TTKEGQYFFDLKVNKDYILEFQSNKIPPTTLEFTTKGITKSDTLVIHDVGIEYLSNKE
                     PFIIKNIYYDFDKYKLRPESQKIIDSTLLVILREAPDIIIELSSHTDSKGDDAYNIEL
                     SQKRAESVINYLIKKGIDKNRLVAKGYGETRPIAPNENPDGSDNPEGRQKNRRTEFRI
                     IGNLSQYSEIIYEE"
BASE COUNT          399 a          212 c          193 g          502 t
ORIGIN      
        1 cctttgagaa tgtttatacg gctcaaatat actaagagtt accaataaaa acaaaatatt
       61 ttacaataaa ctacatttta gctgtttatt cctcataaat tatctctgaa tattgactta
      121 aattaccaat aattctaaat tcagtacgtc tgtttttttg gcggccttca ggattatcag
      181 aaccatcagg attttcgtta ggtgctattg ggcgtgtttc tccatatcct ttagcaacta
      241 aacgattttt atcaattcct ttttttataa gataatttat aacactttct gctcgttttt
      301 gcgataattc tatattgtat gcatcatcac ctttactatc ggtatgactg ctcaattcta
      361 taataatatc cggagcttca cgtagaataa ctaataatgt tgaatctatg attttttgag
      421 attcgggacg taacttatat ttatcaaaat cataataaat attttttata ataaatggct
      481 ctttattaga taaatattca attcctacgt catgtattac aagtgtatcg gattttgtaa
      541 ttcctttagt tgtaaattct agagtagtag gtggtatttt attgctttgg aattccaaaa
      601 tataatcttt gtttactttc aaatcaaaaa agtattgtcc ttcttttgta gttgaatctt
      661 tttgcaacaa tgttcgttct ttatttgttt tatcatgcaa gtagagtgtg acaattgaac
      721 cttcaatata ttttttttgt attgtatcaa ttgctatagt attttttgtt tcaatttgag
      781 attttaaaat atcatgtatt tttttattat atacttcaaa tactttacct gtaacagcta
      841 catttattac tcctaaataa ttaaaagaaa ataaatcatc gcaacaggtt gggtgcgaat
      901 atgaaactgt tccggggcgg tttgatgtaa aaaatccttt tttaccatca tgatgtagta
      961 catagtataa atcatcaaat ggtgagttaa ttgggtagcc aatattttcg gcaggattaa
     1021 atttacccat ctcgcctata ctactgaaaa tatcaagttc gcctaatccg ggccaaccag
     1081 tagagctaaa gtagagagtt cgagttttat tatcataaaa aggggtgagc tcatcgccga
     1141 ttgaatttat tttatttcca caattttttg gttctcgcca cagcttttta cgcggattgt
     1201 atatgctaaa ccatatatca tatccacctt taccaccggg gcggttactt acaaaataca
     1261 atacctcatc gccatttttt aagtaaccgc cccggtggta aaggtg
//
LOCUS       DAOTOW010000708         1304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_82604_length_1304_cov_2.975180, whole genome shotgun sequence.
ACCESSION   DAOTOW010000708 DAOTOW010000000
VERSION     DAOTOW010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82604_length_1304_cov_2.975180"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="P5243_10665"
     CDS             complement(<1..984)
                     /locus_tag="P5243_10665"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19957.1"
                     /translation="MQYGYFDDENKEYVITRPDTPRSWCNYLGDVNYGALITNNAGGF
                     SFYKSAAQGRFMRFRPNTIPMDQPGRYIYIHDKNSKDFWSTSWQPVAKPLEAYKTKTR
                     HGSAYTVIESEYSNIVTETKYFVPLGKHYECWKLTITNNDSTTRNLRLFTYVEYATHW
                     NLHQDIINLQYSQYIVRMDMHNGIINHASNPYLPVVKNNFNAEQSRHTFLALVGADVT
                     GFDTDREVFIGPYRSYNNPLVVEKGECSNSLAQGDNGCGTLQWDIELAPGKTKEIVIL
                     MGIGQAFDEGEQAKREISSPAVAESEFIKVQQHWHNRIQGMTVQTPDSEMNS"
     gene            complement(1086..>1304)
                     /locus_tag="P5243_10670"
     CDS             complement(1086..>1304)
                     /locus_tag="P5243_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRS19958.1"
                     /translation="GKRGDEALYEVKEFIETAHMLQIQKVEILHGKGYGILRKLIRDY
                     VVTTGFVKSCSDAPIDLGGDGITIITLE"
BASE COUNT          398 a          234 c          200 g          472 t
ORIGIN      
        1 cgaattcatc tccgaatcag gtgtttggac agtcattcct tgaattctat tatgccaatg
       61 ttgttgaact tttataaatt cactttcagc aactgcaggt gatgaaattt ctcgttttgc
      121 ttgttctcct tcatcgaatg cttgtccaat acccattaat ataacaattt ctttggtttt
      181 tcccggagca agttctatat cccattgcaa agttccacat ccattgtctc cttgtgctaa
      241 agaattagaa cattctcctt tttcaacaac taaaggatta ttgtatgaac gatatggacc
      301 tataaacact tctctgtctg tatcaaatcc tgttacatcg gcaccaacta atgctaaaaa
      361 tgtatgacga gattgttctg cattaaaatt attttttaca accggtaaat atggatttga
      421 tgcatgatta atgataccat tatgcatatc catgcgaaca atatattgag aatattgtag
      481 gtttataata tcttggtgca aattccaatg tgtagcatat tctacatagg taaacagtcg
      541 taaatttcgt gtagttgaat cattgttggt aattgtaagt ttccaacatt catagtgttt
      601 cccaagtgga acaaaatatt ttgtttctgt aacaatgtta gaatattctg attctattac
      661 ggtatatgct gacccgtggc gcgttttagt tttgtatgct tcaagcggtt ttgcaacagg
      721 ttgccatgat gtactccaaa aatcttttga atttttatca tgaatataaa tatatcgccc
      781 cggctgatcc atgggaatgg tatttggtct aaatcgcatg aatcttcctt gagccgcaga
      841 tttgtaaaaa ctaaatccac cggcgttatt agttattaat gcaccataat ttacatcacc
      901 caaatagtta caccacgaac gaggagtgtc gggccgagta ataacatact ctttattttc
      961 gtcatcaaaa taaccgtatt gcataattta tatgttttaa attttctaca aaataactta
     1021 tttttatgaa tattacgttt gtagtatttt atcaaattgt gtatattttt ctatcaaaaa
     1081 gaaaattatt ctaacgtaat tattgttata ccatcaccac ctaggtcgat aggtgcgtca
     1141 gaacatgatt ttacaaaacc ggttgtaaca acataatcac gaataagttt acgaagtatt
     1201 ccataacctt tgccatgtaa aatttcaact ttttgaattt gtaacatatg agcagtttca
     1261 ataaattctt ttacttcata caatgcttca tcgccgcgtt tacc
//
LOCUS       DAOTOW010000709         1301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_82945_length_1301_cov_2.975120, whole genome shotgun sequence.
ACCESSION   DAOTOW010000709 DAOTOW010000000
VERSION     DAOTOW010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82945_length_1301_cov_2.975120"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="P5243_10675"
     CDS             <1..407
                     /locus_tag="P5243_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19959.1"
                     /translation="FFLSVPSPVKIEPPLGNVQKRNLITQMGEAFKDWADIYFTENRD
                     MLLTKSEVIEDYIRTAKPKHVTSQSFKKSITAWCQFYGYTYNPTELGAGTDGRIIRKK
                     DGKAIENIYIQTKEIDAVQKLLTGDATEEEKF"
     gene            427..672
                     /locus_tag="P5243_10680"
     CDS             427..672
                     /locus_tag="P5243_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19960.1"
                     /translation="MTYQQKIYNFLTAMAPGSSYAVADICKHDTRQLFIDEVKKCISQ
                     HWYSFGWKIDFNADYTVIRRNSSPPDGTFDQFVGHKK"
     gene            732..896
                     /locus_tag="P5243_10685"
     CDS             732..896
                     /locus_tag="P5243_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19961.1"
                     /translation="MTTIAKSNKTVADKSTSKNYKNFEQFEKSMNLPNVEISDDDIMK
                     EVRAVRYGKK"
     gene            938..1144
                     /locus_tag="P5243_10690"
     CDS             938..1144
                     /locus_tag="P5243_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19962.1"
                     /translation="MTTITININEKTVDGKKVLDFLKKSSVEIISMKRKEKKITKSKT
                     YNQVKQSIIDVKKGNVSFVKSINA"
BASE COUNT          539 a          205 c          207 g          350 t
ORIGIN      
        1 agttttttct atctgtacct agtccggtta aaatagaacc gccacttggt aatgtgcaaa
       61 aacgtaacct tattacccaa atgggcgaag cgttcaaaga ttgggcagat atttatttta
      121 cggaaaacag agatatgcta cttacaaaat cagaagtaat agaagactat atacgaaccg
      181 caaaaccaaa acatgtaaca tcgcaatcat tcaaaaaatc gattaccgca tggtgccaat
      241 tctacggtta cacatacaac cctacagaat taggagccgg taccgatggt agaattattc
      301 gaaaaaaaga cggcaaagcc atagagaaca tctatataca aaccaaagaa atagatgccg
      361 tacaaaaact attaaccggc gatgcaacgg aggaggagaa gttttaatgt ttgattggaa
      421 tatacaatga cctaccaaca aaaaatatat aattttctta ctgcaatggc tcccggaagt
      481 tcatatgccg tagcggatat ttgcaagcac gatacccggc aattgtttat tgacgaggtt
      541 aaaaaatgta tatcgcagca ttggtatagt tttggttgga aaatagattt taatgccgat
      601 tatacggtaa ttcgccgcaa ctcttcgcca cccgatggca cgttcgacca atttgttgga
      661 cataaaaaat aatttgcttt taccccacaa aaaaccatat atttgcacta taaacaagaa
      721 aggagcgtag tatgacaaca atagcaaaat caaataaaac cgtagcagat aaatctacta
      781 gtaaaaatta taagaatttt gaacaattcg aaaaatctat gaatctacca aatgtagaaa
      841 tatccgacga tgatataatg aaagaagtaa gagctgtgcg atatggcaaa aaataaaata
      901 aaggttatag cactataaat aagaaaggag tataattatg acaacaatta caataaatat
      961 taacgaaaaa acggttgatg gcaaaaaagt tttagatttt ttaaaaaaat cttctgttga
     1021 aataatttct atgaaaagaa aagaaaaaaa aattacaaaa tccaaaacat acaaccaagt
     1081 aaaacaatca attattgatg taaaaaaagg aaatgtttct tttgtaaaat caataaacgc
     1141 atgaaaataa attttttcga aacacctata tttaatcgtt tagctaaaag gcttggtaaa
     1201 aaatataaat catttcaaat agattttgat aattttttga attctatttc acaacaaaca
     1261 ccaaacaacc ttggaaataa tgtatttaaa tataggtttg c
//
LOCUS       DAOTOW010000710         1299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_83149_length_1299_cov_3.950965, whole genome shotgun sequence.
ACCESSION   DAOTOW010000710 DAOTOW010000000
VERSION     DAOTOW010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83149_length_1299_cov_3.950965"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /gene="rpoB"
                     /locus_tag="P5243_10695"
     CDS             <1..803
                     /gene="rpoB"
                     /locus_tag="P5243_10695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862368.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRS19963.1"
                     /translation="KLKELEGEYKRKVFSVTVGDELPSGIMQLAKVYIAQKRKLKVGD
                     KMAGRHGNKGIVSKVVRDEDMPFLEDGTIVDIVLNPLGVPSRMNLGQIYETVLGWAGK
                     ELGVNFATPIFDGATLDEITEYTRKAGIPDFGKTYLYDGGTGDKFDQPATVGVIYMLK
                     LGHLIDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWAIEAFGASNILQEILT
                     IKSDDVYGRAKAYEAIVKGEPLPVPGLPESFNVLLHELRGLGLNMDFE"
     gene            845..>1299
                     /locus_tag="P5243_10700"
     CDS             845..>1299
                     /locus_tag="P5243_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRS19964.1"
                     /translation="MAFRKDNKIKNNFSKITISLASPEDILERSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTDKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGISTKKLDSIIYYERYVVIQAGTQSDL"
BASE COUNT          466 a          170 c          256 g          407 t
ORIGIN      
        1 aaaaattaaa agaattagaa ggtgaataca aacgtaaagt atttagtgta acagtaggtg
       61 atgaattacc atcaggcatt atgcaacttg caaaagttta tattgctcaa aaacgtaaat
      121 taaaagtggg tgataaaatg gcaggtagac acggtaacaa aggtattgta tcaaaagttg
      181 tacgtgacga agatatgcca tttttagaag atggaacaat agtagatatt gtactaaatc
      241 cacttggtgt accttcgcgt atgaacttag gacaaattta tgaaaccgtt ttaggttggg
      301 caggtaaaga attaggagta aattttgcaa ctccaatttt tgatggtgct actcttgatg
      361 aaattaccga atatactcgt aaagcaggaa tacctgattt tggtaaaaca tatttatatg
      421 atggtggtac aggtgataaa tttgatcaac ctgcaactgt tggtgttatt tatatgttaa
      481 aattaggtca cttaatagac gataaaatgc atgctcgttc tattggtcca tactcgctta
      541 taactcaaca accacttgga ggtaaagctc aatttggagg tcaacgtttt ggagagatgg
      601 aggtatgggc aattgaagca tttggtgcat ctaatatact tcaagagatt ttaacaataa
      661 aatctgacga tgtatatggt agagctaaag cttacgaagc tatagtaaaa ggagaacctc
      721 ttcctgttcc gggtttacct gaatcattca atgtattatt acatgaatta cgcggtttag
      781 gtttaaatat ggattttgaa taaaaaccac ttatattaat tattaaaaaa aaggaattac
      841 atatatggct ttcagaaaag ataacaaaat aaaaaataac ttttcaaaaa tcactataag
      901 ccttgcttct cctgaagata ttttggagcg ttcaagtggt gaagttttaa aaccagagac
      961 tataaattac agaacatata aaccagaacg tgacggttta ttttgtgaac gaatttttgg
     1021 tccggtaaaa gattatgaat gtcattgtgg aaaatacaaa cgtattcgtt acaaagggat
     1081 tgtatgtgat agatgtggag tggaagttac tgataaaaaa gtacgtagag aacgtatggg
     1141 tcatattcaa ttagtagtac ccgtagcaca tatttggtat ttccgttcct tacccaataa
     1201 gataggttac ttacttggta tatctactaa aaaattagat tcaattattt attatgaaag
     1261 atatgttgtt atccaagcag gaacacaatc tgatttgga
//
LOCUS       DAOTOW010000711         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_83871_length_1293_cov_2.075929, whole genome shotgun sequence.
ACCESSION   DAOTOW010000711 DAOTOW010000000
VERSION     DAOTOW010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83871_length_1293_cov_2.075929"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="P5243_10705"
     CDS             <1..627
                     /locus_tag="P5243_10705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS19965.1"
                     /translation="DYYWMTQIQNKIIITAADCTGHGVPGAFMSLLGITFLHEIVHEK
                     QITQSDKILAELRERIIESLNKTGRTEDYKDGMDMALCVIHKDTYTLDYSGAYNPLYI
                     IRDGELIELKADKMPVGLSEKSHDTFTITSYILQSNDCLYMFSDGYVDQFGGPQNRKF
                     MTKQFKELLCLIYHKPMEEQKEILDTTIETWKGTNEQIDDIIVIGLKI"
     gene            632..>1293
                     /locus_tag="P5243_10710"
     CDS             632..>1293
                     /locus_tag="P5243_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRS19966.1"
                     /translation="MWEQSIKEFELYLRLEKSLSPNSIEAYCHDIAKLYEFTQIHAIE
                     IQPHLITTDIIRDFIYWLNMLGIAVRSQARILSGIKAFYAYLWLEKKIETDPTELIDM
                     PKIGKKLPDFLTIQEIDTMLTSIDFSKAEGQRNKAIIETLYSCGLRVSELIELRISNL
                     YFSEGFIKVIGKGNKERLVPISRKAMLEIQYYFESTRNHITPQKGSEDIVFLNRRGKK
                     LT"
BASE COUNT          521 a          183 c          219 g          370 t
ORIGIN      
        1 gattattatt ggatgactca aattcaaaat aaaataataa taacagctgc cgactgtaca
       61 gggcacggag tacccggtgc ctttatgagt ttattgggaa ttacattttt acatgaaatt
      121 gtgcatgaaa aacaaataac acaaagtgat aaaatacttg ccgaattacg agaacgtatt
      181 attgaatcgc ttaacaaaac aggacgaaca gaagattata aagatggcat ggatatggcg
      241 ttgtgcgtaa ttcataaaga cacttatacg cttgattatt caggagcata taatccgctg
      301 tatattataa gagatggtga attaattgaa ttaaaagccg ataaaatgcc ggtaggatta
      361 tcagaaaaat cacacgacac ttttacgata acttcatata ttttgcaatc aaacgattgt
      421 ttatatatgt tttctgacgg atatgttgat caatttggag gtccgcaaaa tagaaaattt
      481 atgacaaaac aattcaaaga actactttgc ttaatatacc ataaaccaat ggaagaacaa
      541 aaagaaatac ttgatactac tattgaaaca tggaaaggta ctaatgaaca aattgatgat
      601 ataatagtaa tagggttaaa aatataatac tatgtgggag caatcaatca aagaatttga
      661 attgtattta cgattagaaa aatcgttatc accaaacagt attgaagcct attgccatga
      721 catagctaaa ctatatgagt ttacacaaat tcatgctatt gaaatacaac ctcatctaat
      781 aactaccgat attataagag attttattta ttggctcaac atgttaggta tagctgtacg
      841 ttctcaagct cgaatattat cgggaatcaa agcattttat gcatatttat ggttagaaaa
      901 aaaaatagaa accgatccta ctgaactcat agacatgcca aaaattggca aaaaattacc
      961 ggacttttta actattcagg aaattgacac catgcttaca tctattgatt ttagtaaagc
     1021 cgaaggtcag cgaaataaag caataattga aactctctac agttgtggat tacgagtaag
     1081 tgaattaata gaattacgaa taagtaattt atattttagc gaaggattta taaaagtaat
     1141 aggaaaaggc aacaaagaaa gacttgtacc aataagcaga aaagcaatgc ttgaaataca
     1201 atattatttt gaatctacaa gaaatcatat aactccgcaa aaaggcagcg aagatattgt
     1261 gttcttgaac cgaagaggaa aaaaattaac aag
//
LOCUS       DAOTOW010000712         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_83919_length_1292_cov_4.303153, whole genome shotgun sequence.
ACCESSION   DAOTOW010000712 DAOTOW010000000
VERSION     DAOTOW010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83919_length_1292_cov_4.303153"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..686
                     /locus_tag="P5243_10715"
     CDS             150..686
                     /locus_tag="P5243_10715"
                     /inference="COORDINATES: protein motif:HMM:NF027596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HRS19967.1"
                     /translation="MLTAKQKRKENIAEYILYMWQLEDILRAYNFNINKLVEQFSYPQ
                     HIKSDLHQWYSEMALAMKNEGVEKSGHLMQLKELVQDLSTLNIQLLQKKDEKEYYELF
                     SDALPYISDLINKSDNKVKNEIDACFTGLYGYMMLKMQNKEISEETHKAMKIFSKLLA
                     RLSFKFHEYEKGELDLDL"
     gene            701..>1292
                     /gene="rpe"
                     /locus_tag="P5243_10720"
     CDS             701..>1292
                     /gene="rpe"
                     /locus_tag="P5243_10720"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRS19968.1"
                     /translation="MAILAPSLLSADFSNLQADIEFVNQSKADWFHLDIMDGVFVPNI
                     SFGLPVVKAIKKHAKKPLDVHLMIVNPDAYIIQFKEAGADLLTVHYEACTHLHRTIQS
                     IKQTGIKAGVSINPHTPVAALQNIITHVDLVLIMSVNPGFGGQSFIPESLQKISELRN
                     LITQTHSKALIEVDGGVDTTNAATIIQKGADVLVAGN"
BASE COUNT          504 a          195 c          213 g          380 t
ORIGIN      
        1 gattttattg aagatataac tcccgctgaa gaattacaaa aattagagat tcttaaatac
       61 gtaaaaccgg tatatagaga acgaggattg ggacgaccaa caaaaaaaga acgaagaagt
      121 ttagaagatt ttggatattt ataaagaaca tgttgactgc aaaacaaaaa agaaaagaaa
      181 atatagccga atatatctta tatatgtggc aattagaaga tatactacgt gcatataatt
      241 ttaatattaa caaacttgtt gaacaatttt catatccgca acatataaaa tcagatttac
      301 atcaatggta ttctgaaatg gctttagcaa tgaaaaatga aggtgttgaa aaatcggggc
      361 atcttatgca actaaaagaa ctggtgcaag atttatcaac attgaatatt caattattac
      421 agaaaaaaga cgaaaaagaa tattatgaat tatttagtga tgcattacca tatatttctg
      481 atttaatcaa caaatcagac aataaagtaa aaaacgaaat agatgcttgt tttacaggtc
      541 tttatggata tatgatgctc aaaatgcaaa acaaagaaat ttcggaagaa acgcacaaag
      601 caatgaagat ttttagtaaa ttgcttgctc ggttatcatt caaatttcac gaatacgaaa
      661 aaggagaact tgatttagat ttataaaaaa ttacattaca atggcaatac ttgcaccatc
      721 attactttca gcagattttt cgaatttaca agccgatatt gaatttgtga atcaaagcaa
      781 agccgattgg tttcatttag atattatgga cggtgtattt gttccaaata tatcatttgg
      841 gttacctgta gtaaaagcta taaaaaaaca cgccaaaaaa cctcttgatg ttcacctaat
      901 gattgtaaat cccgatgcat atattataca attcaaagaa gccggtgccg atttacttac
      961 tgttcattat gaagcttgta ctcatttaca cagaactatc caatcaatta aacaaacagg
     1021 tataaaagcc ggtgtttcta taaatccaca tactccggtt gctgcactac aaaacataat
     1081 tacacatgtt gatttggttt taataatgtc agtaaacccg ggattcgggg ggcaatcatt
     1141 tatacctgaa tcattacaaa aaatttccga attacgcaat ctgattactc aaactcactc
     1201 aaaagcatta attgaggtag atggtggtgt agatactaca aacgcggcaa caattataca
     1261 aaaaggtgcc gatgtattgg ttgccggcaa tg
//
LOCUS       DAOTOW010000713         1282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_84995_length_1282_cov_2.396088, whole genome shotgun sequence.
ACCESSION   DAOTOW010000713 DAOTOW010000000
VERSION     DAOTOW010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84995_length_1282_cov_2.396088"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1241)
                     /locus_tag="P5243_10725"
     CDS             complement(243..1241)
                     /locus_tag="P5243_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HRS19969.1"
                     /translation="MKILYAVQGTGNGHVSRACDVIPELKKYAQVDILISGYQADLQL
                     PFDVTYKLYGMSFIFGKHGGVDIWQTIKRAKIFRFLRDIKRIPVESYDLVINDFEPIT
                     AWACKRKKIPCISVSHQSAVLNPHVPKPPKTDLLGKFVLKYYAPTTKQYGIHFKSYDT
                     NMFTPVIRKDVRNTAPVKNGHYTVYLPAYDDRLLLDIFTQLPEVNWHVFSKHMKIKAV
                     FGNVTIFPVSHKEFGESLITSSGVFCGAGFETPAEALFLGKKLMVIPMKNQFEQQCNA
                     AALKDMGVPVLYSLSEKSIDKISQWIIFGSAISVDYPDVIPPMIEKVIQENLIQNI"
BASE COUNT          440 a          206 c          182 g          454 t
ORIGIN      
        1 tttttatcgg tttcggtaaa ggtatgtgtt tcttgagaaa acccaatatt tataagaatt
       61 atagatatta ttactaataa aaaactttgt ttcattttat cgtttttttt acaatacaaa
      121 gattgcatga gagcaaaaaa aaagaggaaa aacaatatta tgcttttgtt atgataaagt
      181 taaaatcgct tcaaattgct taataattac ttaataaata aacacgatat gttaattaac
      241 tattaaatgt tttgaatgag attttcttgt attacttttt ctatcattgg tggtattaca
      301 tcgggataat caacagaaat agccgaacca aatataatcc attgcgaaat tttatcaata
      361 ctcttttcgc tgagtgaata caaaaccggc actcccatat ctttgagtgc agctgcatta
      421 cattgttgtt caaattgatt tttcataggg attaccataa gtttttttcc taaaaacaat
      481 gcctctgccg gagtttcaaa acctgctccg caaaataccc cactgctagt aattaaactt
      541 tcaccaaatt ccttatgcga aacaggaaaa atggtaacat ttccaaatac agcttttatt
      601 ttcatatgtt tagaaaatac atgccagttt acttcgggca attgtgtaaa aatatctaat
      661 aataatcggt catcataagc aggtaaataa actgtgtaat gaccattttt aacaggggca
      721 gtatttctaa catctttacg aattaccgga gtaaacatat tggtatcata agatttaaaa
      781 tgtataccat attgtttggt tgtaggagca taatatttga gaacaaattt tcctaacaaa
      841 tctgttttag gaggttttgg cacatgtgga ttcaacactg cagattgatg actaacactt
      901 atacatggaa tttttttacg tttacatgcc catgcggtta taggttcaaa atcatttata
      961 accaaatcat atgattcaac aggaatgcgt ttaatatctc gcaaaaaacg aaaaattttt
     1021 gctcgcttaa ttgtttgcca aatatctact cctccatgtt tgccaaatat aaaactcata
     1081 ccatataatt tatatgtaac atcaaaaggc aattgcaagt ctgcttgata tcctgaaatg
     1141 agaatatcta cttgtgcata ttttttaagt tcgggaatta catcacaagc tctacttaca
     1201 tgaccattac ctgtaccctg cactgcatat aaaattttca ttggcatact gtattttttt
     1261 atttatggtt ttaattacta aa
//
LOCUS       DAOTOW010000714         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_85148_length_1280_cov_4.049796, whole genome shotgun sequence.
ACCESSION   DAOTOW010000714 DAOTOW010000000
VERSION     DAOTOW010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85148_length_1280_cov_4.049796"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="P5243_10730"
     CDS             <1..1025
                     /locus_tag="P5243_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19970.1"
                     /translation="KISINGEQLQEIGVGRIPITIIARDKDIAECKSVYEGGIQVSPM
                     PDSKIIGKDKVCEGKTEEFYSVEHGDVQNNYKWEITGKNIMYSKDMTSGATRYVDWGK
                     PGIDTISLMVTSPDLCISSDTLIVYKAKYAEPDFTWELPGAMNIAEFTNKTIQDSIVE
                     KAPDGSIIKLEVPYTMTWDFGRAYNPEGLVVPYKERKEKVRQEYMYGPHDVELTVVND
                     HGCKSVISHQIFVDIKANIQMPNTFAPTNPAYGVRYFQPVAHGLIKCEVWVFDKWGNL
                     LWYSDEVENGIFVGKWDGTYNGEMLQSDTYIWKMEATFADGKQWAGSTKSNGVETKYG
                     TVLLLR"
BASE COUNT          444 a          155 c          255 g          426 t
ORIGIN      
        1 gtaaaatttc tataaatggt gaacaattgc aagaaatagg agttggtaga attcctataa
       61 caattattgc tcgtgataaa gatattgctg aatgtaaatc tgtgtatgag ggtggtatac
      121 aagtttctcc aatgcctgat tcaaaaatta taggtaaaga taaagtatgt gaaggtaaaa
      181 cagaagaatt ttatagtgtt gaacatggtg atgttcaaaa taactataaa tgggaaatta
      241 caggtaaaaa tataatgtat tcaaaagata tgacatcagg agcaactcgt tatgttgact
      301 ggggtaaacc gggaatagat actatttcac ttatggttac atctcctgat ttatgtatct
      361 catctgatac attaattgta tataaagcaa aatatgctga gcccgatttt acatgggaat
      421 taccgggagc tatgaatatt gctgaattta caaataaaac tatacaggat tctattgttg
      481 aaaaggctcc tgatggtagt ataattaaac ttgaagtacc ttatactatg acatgggatt
      541 ttggtagagc atataatccg gaagggcttg tagtaccgta taaagaaaga aaagaaaaag
      601 ttcgtcaaga atatatgtat ggtccgcacg atgttgaatt aacagttgtt aatgatcatg
      661 ggtgtaaaag tgtaatctca catcaaatat ttgttgatat taaagcaaat attcaaatgc
      721 ctaatacgtt tgcaccaacc aaccctgcgt atggagttcg gtattttcaa ccggttgctc
      781 atggtcttat aaaatgtgaa gtatgggttt ttgataaatg gggtaattta ctgtggtatt
      841 cagatgaagt tgaaaatgga atatttgtag gtaaatggga tggaacatac aatggtgaaa
      901 tgctgcagtc tgatacctat atatggaaaa tggaagcaac atttgctgat ggtaaacaat
      961 gggcaggttc tacaaaatct aatggtgtag aaacaaaata tggaacagta cttctcctaa
     1021 gataataaat caaaattatt gtaaacctct gtaataattg taattacaga ggtttttttt
     1081 atttgttgtt gggagtttta ataattattt tggtaagaga tttctaaaga ttgatatata
     1141 tcgttgtttt tgagattcaa tggaataata attttctata gttttacgtc ctgcaattcc
     1201 aatacgtttt cttaattccg catcttgtat aagaatagtt aagtaatgtt cccattcagc
     1261 ttgggtgctt gctaaatatc
//
LOCUS       DAOTOW010000715         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_85163_length_1280_cov_3.097959, whole genome shotgun sequence.
ACCESSION   DAOTOW010000715 DAOTOW010000000
VERSION     DAOTOW010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85163_length_1280_cov_3.097959"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="P5243_10735"
     CDS             complement(<1..588)
                     /locus_tag="P5243_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HRS19971.1"
                     /translation="MIEQIVNPNNVMRALQQVKQNGGSAGIDRMPVAELYAHITKNRK
                     QMIDSIRTGTYQAQAIKGVTILKANGKTRLLGIPTVTDRMWQQATAQILALRFEFKFH
                     DHSYGFRPNKKAQHAVLQAQEYINSGYKHIVDIDLQTFFDEVDHCILLNLIYQKVQCP
                     KTLRLIRKWLKAPIQINGKLTKRTKGVPQGSPLSPL"
BASE COUNT          287 a          266 c          260 g          467 t
ORIGIN      
        1 taacggactc agtgggctgc cttgcggtac tcctttggtt cgtttagtta gtttgccgtt
       61 gatttgtata ggtgctttga gccatttacg tataagtcgc aatgttttgg ggcattgtac
      121 tttttggtaa atcagattta gtagtatgca atggtctact tcgtcgaaga atgtttgtag
      181 gtctatatcc actatgtgtt tatatccgct atttatgtat tcttgtgcct gtagtactgc
      241 atgttgtgcc tttttgttgg gtctaaatcc ataactgtgg tcatggaatt tgaactcaaa
      301 cctaagggca aggatttgtg ctgtggcttg ttgccacatt cggtcggtta cggtaggaat
      361 gcccagtaga cgtgtcttgc cgtttgcctt gaggatagtt actcctttga ttgcttgggc
      421 ttggtatgtc cctgtgcgga ttgaatcaat catctgtttc ctatttttgg taatatgtgc
      481 atatagttca gctaccggca ttctgtctat tccggcagaa ccaccgtttt gctttacttg
      541 ttgtaatgct cgcattacgt tgttaggatt tacaatttgt tcaatcatac catgttgttt
      601 ttgttgtttg tactccgctt cgcttagtgt atggcttgtg aaaaaaaaaa tatacatcca
      661 tacataattt gaagcacctt attattcagt ccttcgcacc tgtgtttctc tgtcggatag
      721 ctcgggggta cctgttcgct cggctcccgc agacatatct ttcggcttgc cccgatttac
      781 agtttgtttg aagtatttga aaaatactga tatacaaaat gttaatcgat tacgcgattt
      841 ctattgcgta atttgggctt atactacaca acatactact atgacctctg ctgacttctt
      901 gttgtgagta acccgtaccc tacaagacct ccctcggtaa gtgtgttttc cttcgttcaa
      961 tacccgtagc atctatcccg ataatagttt ttgttgattt tgctcatgca aaatcttaca
     1021 aaacttaaat cgggacctca tatgctcttc ctattcgtcg gtaccgaact ttgcagtctc
     1081 gcttccttca ctgcttgcat cactgcaaac cagcttgcga cttgctaatg gttagggcac
     1141 taatcctacc catgacggac ttgcaccgct tggaaaaatt tttttacaca tagtgtttgt
     1201 tttatttaga aaaatttgta tttttccaat ttgttaaaac ttgcactacg tgtgtatcgc
     1261 acacatgcga ggcacacaca
//
LOCUS       DAOTOW010000716         1270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86210_length_1270_cov_3.128395, whole genome shotgun sequence.
ACCESSION   DAOTOW010000716 DAOTOW010000000
VERSION     DAOTOW010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86210_length_1270_cov_3.128395"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1270)
                     /locus_tag="P5243_10740"
     CDS             complement(<1..>1270)
                     /locus_tag="P5243_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19972.1"
                     /translation="DGLWMRTHDLRKVKSPRYADCVVPRPTYKYGQNTLAEPGGDAAS
                     DGDFDIALALLMAWYQWGDNMGINDACGNPISYKKEAIDVIKGLVEITNSGLGDCRST
                     TGNIGFDGYVKNGNTWGETTNFSNTRTPECPEFKGPQQMHVDYMAPAYFRAFREFLTA
                     QGITGWEVDQLLKAEASSDWIVGEWIYKNARNIPVGGWVDIVGNSPTWSTFMQGEDFR
                     APWRTILNYVWHGNPTYKWNPTTHTVTQSSPNTYEKDAAVRYSQYLKDPQGTPWNNPC
                     VAFGGGPSLTYKGPATLQWEISPTGAGTSAFTLNWIPGTGAPAAVGAQDYDLMGLLYR
                     QCAIEWDVSVGGDRYLTSIPVYFHGWFRLLGLLVTTGNAHTPTNMLPKANLKVYRAIN
                     GGVTYAFTGDNITYKLSYRNYGSVDATNSV"
BASE COUNT          357 a          279 c          219 g          415 t
ORIGIN      
        1 attaccgaat ttgtagcatc tacagaacca tagtttctat aacttaattt atatgttata
       61 ttgtcgccgg taaatgcata ggttacacca ccatttatag ctctatatac ttttaagtta
      121 gctttgggaa gcatatttgt tggagtatgt gcatttcctg ttgttactaa taaacctaat
      181 aaacgaaacc aaccatgaaa ataaaccgga atacttgtca aatatctatc gcctccaaca
      241 ctaacgtccc attctatagc acattgtcga tagagtaatc ccattaaatc ataatcttga
      301 gcacctactg cagccggagc tcctgtgccg ggaatccaat tgagtgtaaa tgccgaagtc
      361 cctgctcctg ttggtgatat ttcccattgt aatgtagccg gtcctttgta tgttaaactt
      421 ggtccaccac caaatgctac acatgggttg ttccatggtg ttccttgagg atcttttaaa
      481 tattgagaat atcgaaccgc agcatctttt tcatatgtat tggggctgct ttgggtaaca
      541 gtatgggttg ttggattcca tttatacgta ggatttccgt gccatacata atttaaaatt
      601 gttctccatg gagcacgaaa atcttcacct tgcataaatg tagaccatgt aggactatta
      661 cctacaatgt caacccatcc gcctacagga atatttcgag catttttata tatccattct
      721 cctacaatcc agtcactcga agcttcagct tttaacagtt gatctacttc ccaaccggta
      781 ataccttgtg cggttaaaaa ttcacgaaat gctctaaaat atgccggagc catatagtct
      841 acgtgcattt gttgaggacc tttaaattca ggacattccg gtgttcgtgt atttgaaaaa
      901 ttagtggttt caccccatgt attaccattt tttacgtagc cgtcaaaacc aatatttccc
      961 gttgtgcttc tacagtcgcc taaaccactg tttgtaattt caactaatcc ttttataaca
     1021 tcaatagctt cttttttata acttattgga ttgccgcagg catcatttat tcccatgtta
     1081 tcgccccatt gataccatgc catgagtaat gctaatgcta tatcaaagtc tccgtctgaa
     1141 gcagcatctc caccaggttc ggcaagtgtg ttttgaccat atttatatgt tggacgggga
     1201 accacacaat ccgcatagcg aggtgattta actttacgta aatcatgagt acgcatccat
     1261 aagccatcaa
//
LOCUS       DAOTOW010000717         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86316_length_1269_cov_3.368204, whole genome shotgun sequence.
ACCESSION   DAOTOW010000717 DAOTOW010000000
VERSION     DAOTOW010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86316_length_1269_cov_3.368204"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="P5243_10745"
     CDS             <1..361
                     /locus_tag="P5243_10745"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRS19973.1"
                     /translation="ELATANHVLLVYSHNMMHVVEGNYESIGGIFSMRKAPEFKNVSI
                     PIEPRMRIYMFSDGIQDQFGGPKNEKFTRARLEKLLLDTTSLPIKEQGKKVEQEFTVW
                     KEGKAQLDDVLLLGIQF"
     gene            371..>1269
                     /locus_tag="P5243_10750"
     CDS             371..>1269
                     /locus_tag="P5243_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS19974.1"
                     /translation="MFQKNVLIFFFVFVVTCVYAGVEKMYSVDTLLITNKEGLIQSHK
                     EVISILNATNVSKKAELFYNTGYALANKPKATKKDFLVAISYISIAAEFYKESGSYKE
                     FGTCLENLATIHYELSEYEKAVSLFEESIEIHKTYLSHDIAHIALLHTNIAFIFFNTY
                     RYENAIRFFSNSLLYYTTIKDTVGIGKTLYYKGLVFYENNELDSALVYYEKALKYDLL
                     SKNVNEILASYNNLAVLYTKKRSLNQAISYLDKSLGLIEKIDNNQSQLSMYHINIGNV
                     YFQKKQFDKALESYNAALQLKRD"
BASE COUNT          474 a          146 c          203 g          446 t
ORIGIN      
        1 tgaattagct acagctaatc atgtgttatt agtatattct cataacatga tgcatgttgt
       61 agaaggaaat tatgaatcaa ttggtggtat tttttcaatg cgaaaagcac cggaatttaa
      121 aaatgtatct ataccaattg aacctcgtat gcgaatttat atgttttctg atggtattca
      181 agatcaattc ggtggtccta aaaatgaaaa atttacccgt gctcggttgg aaaaactatt
      241 gcttgataca acatcgcttc ctataaaaga acaaggtaaa aaagtagagc aagaatttac
      301 tgtttggaaa gaaggtaagg ctcaacttga tgatgtattg ttacttggta ttcaatttta
      361 aacctattgt atgtttcaaa aaaatgtatt gattttcttt tttgtttttg ttgtaacttg
      421 tgtgtatgca ggtgtcgaaa aaatgtattc tgtagataca ttacttatta caaataaaga
      481 aggtttaata caatcacata aagaagttat ttcaattcta aatgcaacca atgttagtaa
      541 aaaagcagag ttgttttata acaccggata tgctttagct aataaaccta aagcgactaa
      601 aaaagatttt ttggttgcaa tttcctatat ttcaatagca gcagagtttt ataaagaatc
      661 cgggagttat aaagaatttg ggacctgttt ggaaaattta gcaaccattc attatgagtt
      721 gagtgaatat gaaaaagctg tatcgttgtt tgaagagtca atagaaattc ataaaacata
      781 tttatctcat gatattgctc atatagctct tttacataca aatattgctt ttatattttt
      841 taatacgtat agatatgaaa atgcaattcg ttttttttca aattcattat tatattatac
      901 tacaataaaa gatacagtag gtattggaaa aacattgtat tataaaggct tggttttcta
      961 cgaaaacaat gaacttgata gcgcattggt atattatgaa aaagccttga aatatgattt
     1021 attaagtaaa aatgtaaatg aaattcttgc atcatacaat aatttagctg ttttatatac
     1081 aaaaaaaaga agtcttaatc aagcaatttc atatttagat aaatccttag ggttaattga
     1141 aaaaattgat aataatcagt cgcaattatc aatgtatcat ataaatattg gtaatgtata
     1201 ttttcaaaaa aaacaatttg ataaggcatt agaaagttat aatgcggcat tgcagttaaa
     1261 aagagatat
//
LOCUS       DAOTOW010000718         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86340_length_1269_cov_2.452224, whole genome shotgun sequence.
ACCESSION   DAOTOW010000718 DAOTOW010000000
VERSION     DAOTOW010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86340_length_1269_cov_2.452224"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1203
                     /locus_tag="P5243_10755"
     CDS             178..1203
                     /locus_tag="P5243_10755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS19975.1"
                     /translation="MIDVLIIGGGIAGIETACSLQELGLETLVLEKEKQIGGHLNNWD
                     TLFPTKRSASEILEYYAEQIRKKSITIYLNTTVTSIRKQDDGSFEVHTSREVSFKSKT
                     VVMAHGFSLFNAQRKEEYGYKIYENVITSADLEQTFKQNNDVFVNPMGKIPERVAFIH
                     CVGSRDEKSGNHYCSKVCCITAVKQAIKVKQLSPQTQVFCFYMDLRLYGRGFEEMYRE
                     AQEKWGVQFIRGRLSEVAENIDGSLQLKAEDTLAARPFKMSVDMLVLMVGFEANKSTA
                     LFHNSCGVACSESRFIQPRHHHLSANATNIDGLFTAGTSIAPLSVFDTLNHAKAAAFA
                     IHSYITK"
BASE COUNT          451 a          177 c          244 g          397 t
ORIGIN      
        1 gctgcattgg tattaggaat acaaccatgg gatgtaggat tacaaataca tcaaatacaa
       61 agtgaatcac ttcttgatgc tttaggaata ccatatgata ctgcattaaa atatgttgat
      121 aaacaaggta atcaattacc attaccaaat aaacctaaca atttattgca ataaattatg
      181 atagatgtac ttataatagg tggaggtata gccggtatag aaacggcttg ttctttacaa
      241 gaattgggtt tagaaacctt agtcctagaa aaagaaaaac aaataggagg acatttaaat
      301 aattgggata cattgtttcc tacaaaacgt tctgcatcag aaatattaga atattatgca
      361 gaacaaatta gaaaaaaatc tattacaata tatttgaata ctacagtaac ttcaataagg
      421 aaacaagatg acggcagttt tgaggttcat acgtctcgtg aagtttcatt taaaagtaaa
      481 accgtagtta tggctcacgg attttctttg tttaatgctc agcgaaaaga agaatatgga
      541 tacaaaatat atgaaaatgt aataacatct gccgatttgg aacaaacgtt taaacagaat
      601 aacgatgtgt ttgtaaaccc gatgggaaaa atacccgaac gtgttgcgtt tattcattgt
      661 gtagggagtc gcgacgaaaa atcgggtaat cattattgtt caaaagtttg ttgtattaca
      721 gcggtaaaac aagcaattaa agtaaaacaa ttgtcaccgc aaacacaagt tttttgtttt
      781 tatatggatt tacgattgta cggtagagga tttgaagaaa tgtatcgtga agctcaagaa
      841 aaatgggggg ttcaatttat tcgtggacgt ttgtcggaag tagcagaaaa tattgatgga
      901 agtttgcaat tgaaagctga ggatacactt gctgcacgac cttttaagat gtcggttgat
      961 atgttggtgc ttatggtagg atttgaagca aataaaagta ctgcattatt tcataattcg
     1021 tgtggtgttg catgttccga aagtcgtttt atacaaccac gtcatcatca tctttcggca
     1081 aatgcaacta atatagatgg attatttacc gcaggtacca gtattgctcc tctttcggta
     1141 tttgatacac tcaatcacgc taaggctgca gcattcgcta ttcattcata tattacaaag
     1201 taattttttt atgatgaaac aatttggata taattcgttg aaaagtagaa ctattgatta
     1261 taaaaatat
//
LOCUS       DAOTOW010000719         1268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86453_length_1268_cov_2.484749, whole genome shotgun sequence.
ACCESSION   DAOTOW010000719 DAOTOW010000000
VERSION     DAOTOW010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86453_length_1268_cov_2.484749"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="P5243_10760"
     CDS             complement(<1..860)
                     /locus_tag="P5243_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS19976.1"
                     /translation="MKKKQIILNILLGLVISFGIFSCKPKSNTVAPPTFTINSDAYNF
                     YVVSDVGRNGYFKQKSLADTMGSFSERISPKFIISAGDCFHGLGVRSVQDPIFLTNFE
                     NIYTHPNLHCEWYPILGNHEYQGNTQALIDYSQISRRWCMPDRYYSITKKVSDSTMLK
                     IVFIDTPPFVEKYRKETEKYPDACKQDIQKQLVWLEKTLAEAKEKWILVVGHHPIYSI
                     DEKHGDTPELIEKVKPLLQKYKVDAYICGHIHNFQHLQIPDSGFEYFVNSSASLGRPN
                     FENDSTKFTS"
     gene            complement(878..>1268)
                     /locus_tag="P5243_10765"
     CDS             complement(878..>1268)
                     /locus_tag="P5243_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HRS19977.1"
                     /translation="YYSDEKFGIRKYYADADKGNKELAVFGTSGFTSDQEGISIYTQP
                     NGEGYIIVSDQQANQFHVFSRKGTVSNEHEHKLVHIVKTKTDESDGSDLCSLPLNATF
                     TQGIFVAMSSDKTFQFYKLEDIVPIKK"
BASE COUNT          413 a          206 c          174 g          475 t
ORIGIN      
        1 tttgatgtaa atttagttga atcattttca aagttaggtc gtcctaatga agctgatgaa
       61 tttacaaaat attcaaaacc actatcagga atttgtaaat gctgaaaatt atgaatatgc
      121 ccacatatat atgcatctac tttatacttt tgtaaaagtg gtttaacttt ttcaattaat
      181 tcaggagtgt ctccatgttt ttcatcaata gaatatattg gatgatgtcc tacaaccaga
      241 atccattttt ctttggcctc tgcaagagtt ttttctaacc atactaattg tttctgaatg
      301 tcttgtttac atgcatcagg atacttttcg gtctcttttc tatatttttc tacaaacgga
      361 ggagtatcaa taaatacaat tttgagcatt gtactatcac ttactttttt tgtaattgaa
      421 taatatctat caggcataca ccaccgtcta cttatttgcg aataatcaat gagagcttga
      481 gtattgcctt gatattcatg attccccaat attggatacc attcacaatg taaatttggg
      541 tgtgtatata tattttcaaa atttgttaaa aatattggat cctgaacact tcgaacaccc
      601 aatccatgaa aacaatcacc ggccgaaata ataaatttag gtgaaattct ttcagaaaaa
      661 cttcccattg tatcggctaa acttttttgt ttaaaatacc catttctgcc tacatctgaa
      721 actacataaa agttatatgc atcgctattt atagtaaatg ttggaggggc aacagtatta
      781 gatttaggtt tacacgagaa tattccaaaa cttataacta aacctaaaag aatatttaga
      841 attatctgtt tttttttcat aactaaaaat gtttttatta tttttttatt ggaacaatat
      901 cttctaattt ataaaattga aatgttttat ctgatgacat tgcaacaaaa ataccttgag
      961 taaaagttgc atttaacggc aaactgcata agtctgaacc atcactttca tctgttttag
     1021 tttttacaat gtgcacaagt ttatgttcat gttcattgct aacagtacct tttcgtgaaa
     1081 atacatgaaa ttgatttgct tgttggtcag aaacaataat atatccctcg ccatttggtt
     1141 gtgtatatat cgaaattcct tcttgatcag atgtaaaacc tgatgtgcca aatacagcta
     1201 attctttatt tcctttatca gcatcggcat aatatttacg gataccgaat ttttcatctg
     1261 aataataa
//
LOCUS       DAOTOW010000720         1265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86722_length_1265_cov_3.838017, whole genome shotgun sequence.
ACCESSION   DAOTOW010000720 DAOTOW010000000
VERSION     DAOTOW010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86722_length_1265_cov_3.838017"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..454
                     /locus_tag="P5243_10770"
     CDS             50..454
                     /locus_tag="P5243_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19978.1"
                     /translation="MKRILIISAVLFVTIAAFAQKPKDGIEVLYFKANLACCMARACN
                     ALEADVQKVITTNYPNGEVTFIEVKLADPANEALVAKYETKSQTVVIVKTKRGKQTHA
                     DVSAITREYLRTQDYSAFEKALVAKIEEIKKK"
     gene            473..1177
                     /locus_tag="P5243_10775"
     CDS             473..1177
                     /locus_tag="P5243_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRS19979.1"
                     /translation="MDFLQSILHNTQYASITAIVLGLMTAISPCPLATNITAIGFISK
                     DIADKRRVFVSGIVYTFGRAFSYTLLAVILFLGASKINISRIFQGWGDILIGPVLLII
                     GLFMLGIFRFSIPGFGNVTEKIGERLQGSYWGAFILGVLFALAFCPYSGVLYFSMLIP
                     ITIASVNGLYLPLLFAIATGIPVIIFAWVLAYTISNVGVLYNNVKRFELWFRRVVAIV
                     FICVGLLYIYKYFFYV"
BASE COUNT          419 a          142 c          226 g          478 t
ORIGIN      
        1 taaaaaaata attagagata ctattcaatc acttttaaaa taattattta tgaaacgaat
       61 attaattata agtgcagttt tatttgttac tattgcagca tttgctcaaa aacctaaaga
      121 tggtattgag gtattgtatt ttaaagcaaa tttagcatgt tgtatggctc gtgcatgtaa
      181 tgcattagaa gcagatgttc aaaaagtaat tacaacaaat tatccgaatg gggaagtaac
      241 ttttatcgaa gttaaacttg cagatccggc aaatgaagca ttagttgcaa aatatgagac
      301 aaaatcgcaa accgttgtta ttgttaaaac taaacgaggt aaacaaacac atgcagatgt
      361 ttctgcaata actcgtgaat atttaagaac acaagattat tctgcgtttg agaaagcatt
      421 ggttgctaaa attgaagaaa ttaagaaaaa atagtttctt atatgaaaca atatggattt
      481 tttacaatca atattacata acacacaata tgcttctata acagctattg tattaggttt
      541 aatgacagca ataagccctt gtccgcttgc tactaatatt acagctatag gttttataag
      601 taaagatata gcagataaac gaagagtatt tgtaagtgga atagtatata cttttggtag
      661 agcttttagt tatactcttt tagctgtaat acttttttta ggagcaagta aaattaatat
      721 ttcacgaatt tttcaaggat ggggagatat tttaataggt cctgttcttc ttattattgg
      781 tttatttatg ttaggtattt ttcggttttc aatacccgga tttggaaatg taaccgaaaa
      841 aataggtgag cgtttgcaag gatcatattg gggagcattt atattaggag tattgtttgc
      901 tttggcattt tgtccatata gtggagtttt atatttttca atgcttatac ctattacaat
      961 tgctagtgtt aatggattgt atttaccttt actttttgct atagctacag gaattccggt
     1021 tattattttt gcatgggtac ttgcttatac tattagtaat gtaggagttt tatacaataa
     1081 tgttaaacgt tttgagcttt ggtttcgtcg tgttgttgct attgtgttta tatgtgttgg
     1141 attattgtat atatataagt atttttttta cgtataaaat gattgtaaat tttggcgatg
     1201 agtagtatca gtaaaaggat tcaaacgatt agttcaatta ttataatagt acctttatta
     1261 atagg
//
LOCUS       DAOTOW010000721         1265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86764_length_1265_cov_2.205785, whole genome shotgun sequence.
ACCESSION   DAOTOW010000721 DAOTOW010000000
VERSION     DAOTOW010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86764_length_1265_cov_2.205785"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="P5243_10780"
     CDS             <1..719
                     /locus_tag="P5243_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRS19980.1"
                     /translation="IKNAANAIASIEFGTIMNKVVSTSKSSAAHTAGEWLEKHHSVPN
                     TRMPLPIGIEASDMFFKTLETISGIATPRHHARERGRLIDAYVDGHKYVFGKRAVVFG
                     EEDMVLSMVAFLHEIGIETVLAGSGGESNSLKKEIDARTGGVYPNMIVMNNLDFETIN
                     EMVDELKPDIFIGNSKGYYIARRLKKPIVRIGFPIHDRFGAQRLHHIGYRGTQELFDR
                     IVNALIEYKQEHSPVGYKYI"
     gene            779..>1265
                     /locus_tag="P5243_10785"
     CDS             779..>1265
                     /locus_tag="P5243_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS19981.1"
                     /translation="METIAKHPCFDKEAKHTHARVHLPVAPKCNIQCNYCNRKFDCVN
                     ESRPGVSSAVLSPYQALEYVKELNTKLENLSVIGIAGPGDPFANATETMETMRLLKAE
                     FPEKLFCVSTNGLNLEPFIPELAELGVSHVTITINAVDPDITSKIYAWVRDGVTIYRN
                     RQ"
BASE COUNT          437 a          233 c          261 g          334 t
ORIGIN      
        1 aaataaaaaa tgctgctaat gctatagcaa gtatagaatt tggcaccatt atgaataaag
       61 tagtgagtac ttccaaatcg tcggcagctc atactgccgg cgaatggttg gaaaaacacc
      121 actcggtgcc aaatacccgt atgcctttac ctattggtat tgaagcaagc gatatgtttt
      181 tcaaaacatt agaaactata agcggtattg caaccccacg gcatcatgca cgcgaacgcg
      241 gacgattgat tgatgcctat gttgacggtc ataaatatgt atttggcaaa cgtgccgtgg
      301 tatttggtga agaagatatg gtattgagta tggtcgcatt tctccacgaa ataggtatag
      361 aaacagtatt ggcagggtcg ggtggcgaaa gtaattcgct caaaaaagaa attgatgcac
      421 gcaccggtgg agtatatccc aacatgatag taatgaacaa ccttgatttt gaaacaataa
      481 acgaaatggt tgacgaactg aagcctgata tttttatagg taacagcaaa ggctattata
      541 tagcacgcag gctcaaaaaa ccaatcgtac gtataggatt tcccatacac gaccgctttg
      601 gagctcaacg cttacatcat attggatacc gaggaacaca agaattattc gatagaatag
      661 taaatgccct catagaatat aaacaagaac attcgccggt aggctataaa tacatataat
      721 ggttaattgt taattattaa tgattaatag agtgataaga aataaaaata atacaactat
      781 ggaaacaatt gcaaaacatc cgtgtttcga caaagaagca aaacacacac atgctcgagt
      841 acacttaccg gtagctccca aatgtaatat acaatgcaat tattgcaacc gtaaattcga
      901 ctgtgtaaac gaaagtcgtc cgggtgtttc gtctgccgta ttaagtccat accaagcatt
      961 ggaatatgta aaagaactca atactaagtt agaaaattta tcggtaatag gtattgcagg
     1021 ccccggtgat ccgtttgcca atgcaaccga aactatggaa actatgcgat tgttgaaagc
     1081 tgaatttccc gaaaaactat tttgcgtgtc aaccaatggc ttgaatttgg aaccattcat
     1141 tcccgaattg gcagaattgg gtgtgagcca tgttacaata accataaatg cagtagaccc
     1201 cgatattacg tcaaaaatat atgcgtgggt acgcgatgga gttactattt atcgtaatcg
     1261 tcaag
//
LOCUS       DAOTOW010000722         1264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86859_length_1264_cov_2.765095, whole genome shotgun sequence.
ACCESSION   DAOTOW010000722 DAOTOW010000000
VERSION     DAOTOW010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86859_length_1264_cov_2.765095"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="P5243_10790"
     CDS             complement(<1..968)
                     /locus_tag="P5243_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS19982.1"
                     /translation="MIEEDDSFDSLFAEEDEPIAESTQNEQVWKILLVDDEQDIHSAI
                     RLALDNFTYEGKKIKFFDAYSGEEAKQILQQNTDIALILLDVVMETSHAGLDFVKFIR
                     NDLQNHFIRVVLWTGQPGYAPKKDVILSYEINDYKTKTDLTDDNIFTAVLASIRAYNA
                     IMTVESYRQNLEQKVIERTKTIEEQKKKIVDSISYAEKIQRSILPSQEVFSKYFPESF
                     ILFKPRDVVSGDFYWLSQVDENVIFVATADCTGHGVPGAFMSMIGNTLLNEIVATKEV
                     QSPASILSQLNAGIIDALTKHTNSEDAQTDGMDITLCKIDLHNNII"
     gene            complement(1070..>1264)
                     /locus_tag="P5243_10795"
     CDS             complement(1070..>1264)
                     /locus_tag="P5243_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19983.1"
                     /translation="HPYLPKQTVIFFCYTICSHISVISTAQCSVLSGLSSSKEAIEQP
                     TDLQIYYNSNYLLCGLMFWI"
BASE COUNT          408 a          212 c          187 g          457 t
ORIGIN      
        1 tcaattatat tattgtgtaa atcaatttta catagtgtaa tatccatgcc atcggtttga
       61 gcatcttcag aatttgtatg ttttgtaaga gcatctataa tacccgcatt taattgtgaa
      121 agaatagatg ccggactttg aacttcttta gttgctacta tttcattaag caaagtattt
      181 cctatcattg acataaaagc tccgggaaca ccgtgaccgg tacaatctgc tgttgcaaca
      241 aaaataacgt tttcgtctac ttgcgataac caataaaaat caccacttac aacatcacgc
      301 ggtttaaaca aaataaaaga ttctggaaaa tatttagaaa aaacttcttg tgagggtaat
      361 attgaacgtt gaattttctc ggcatatgat atactatcta ctattttttt cttttgttct
      421 tcaatagttt ttgtacgttc tattactttt tgttccaaat tttggcgata cgattctaca
      481 gtcattatag cattgtaagc tctaatactt gccaatactg ccgtaaatat attatcatcg
      541 gttaagtctg tttttgtttt gtaatcgttt atttcatacg ataatattac atctttttta
      601 ggagcatatc ccggttgacc agtccataat acaactcgta taaagtgatt ttgcaaatca
      661 ttacgaataa atttcacaaa atcaagtccc gcatgacttg tttccatcac tacatcaaga
      721 agtataagag ctatatcggt attttgttgt aatatttgtt ttgcttcttc acctgaatac
      781 gcatcaaaaa atttaatttt cttaccctca tacgtaaaat tatccaaagc taaacgtata
      841 gccgaatgaa tatcttgttc atcatctact aataaaatct tccacacttg ttcgttttgt
      901 gtgctttctg ctataggttc gtcttcttcg gcaaaaagtg aatcaaaact atcgtcttct
      961 tctatcattg ttttagtatt tgtatgctgt tttattcagc taatatacaa aaaaaatacg
     1021 agcgttgtat atttttatta aaaattaact attctctaaa agaattgatt tatatccaaa
     1081 acatgagacc acaaagtaaa taattagaat tatagtatat ttgcaaatca gtaggttgtt
     1141 ctatcgcttc ttttgaagag gaaagtccgg acaacacaga gcattgtgct gttgaaataa
     1201 cagatatgtg tgaacatata gtatagcaga agaaaataac cgtttgcttc ggcaaataag
     1261 ggtg
//
LOCUS       DAOTOW010000723         1263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_86982_length_1263_cov_2.198675, whole genome shotgun sequence.
ACCESSION   DAOTOW010000723 DAOTOW010000000
VERSION     DAOTOW010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86982_length_1263_cov_2.198675"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="asnB"
                     /locus_tag="P5243_10800"
     CDS             complement(<1..1202)
                     /gene="asnB"
                     /locus_tag="P5243_10800"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HRS19984.1"
                     /translation="MCSVLGIFDLKVESQKLREQALQMSRKLRHRGPDWSGIYCGDRA
                     ILAHERLSIVDPQSGKQPLYSKDKKLILAVNGEIYNHREIRAKTEQDYEYQTHSDCEV
                     ILALYKQKGTDFIEDLNGIFAFALYDEEQDRYFIGRDHIGIIPLYMGWDKLGNFYVSS
                     ELKGLEGYCNRIEEFLPGHTLDSKEGVLKKWYTRDWMEYDAVKNNTTSIDELREALEN
                     AVYRQLMSDVPYGVLLSGGLDSSITSALAKKYSRTRIETNGKQEAWWPQLHSFAVGLV
                     GSPDLAAAKKVADHIGTIHHEVNFTVQEGLDAIKDVIYHIETYDVTTIRASTPMYLLA
                     RVIKSMGIKMVLSGEGADELFGGYLYFHKAPNPEEFHKETVRKLSKLHMYDCLRANKT
                     LAAWGVEGR"
BASE COUNT          369 a          245 c          193 g          456 t
ORIGIN      
        1 cgaccttcaa caccccatgc tgcaagtgtt ttgttagctc gcaaacaatc atacatatga
       61 agtttactca atttgcgtac agtttccttg tgaaattctt cgggatttgg agctttgtga
      121 aaatataaat atccaccaaa taattcatcg gcaccttcac ctgataatac cattttgata
      181 cccattgatt tgattacacg tgcaagtaaa tacatgggag tacttgctct aattgttgta
      241 acatcatatg tctcaatatg gtatattaca tcttttatag catctagtcc ttcttgtact
      301 gtaaaattta cttcgtggtg tatggttcca atgtggtcgg caactttttt tgctgcggct
      361 aaatcgggtg aacctactaa accaacagca aatgaatgta attgaggcca ccaagcttct
      421 tgttttccgt tagtttctat tctggtacgt gaatattttt ttgctaatgc agatgtaatt
      481 gaagagtcaa gaccacctga taaaagtaca ccgtaaggta catcactcat aagttgacga
      541 tatacggcat tttctaaagc ttcgcgtagt tcatcaatac ttgttgtgtt gttttttaca
      601 gcatcatatt ccatccagtc gcgagtatac cattttttta gtacaccttc tttactgtca
      661 agtgtatgcc ccggtaggaa ttcttctatt cggttacaat atccttcaag tccttttaat
      721 tctgatgaaa cataaaaatt acctaatttg tcccacccca tgtataatgg tataattcct
      781 atatgatctc ggccaataaa atatcggtct tgttcttcat catataaagc aaatgcaaaa
      841 attccgttta aatcttcaat aaaatcggta cctttttgtt tatataatgc taaaattact
      901 tcacaatcag aatgagtctg atattcatag tcttgttctg tttttgctcg tatttctctg
      961 tgattatata tttcaccgtt tactgccaat attaattttt tatctttact gtacaatggt
     1021 tgttttcccg actgtgggtc aacaattgaa agtctttcgt gagctaatat tgcacgatcg
     1081 ccacaataaa taccagacca atccggacca cgatgacgta attttcgtga catctgtaag
     1141 gcttgttcgc gtaatttttg tgactcaact tttaaatcaa aaattcctaa tacactacac
     1201 atactgtttc tatcttatta attatttgaa agcaaaatta cattatgaaa gtttaaatag
     1261 caa
//
LOCUS       DAOTOW010000724         1256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_87720_length_1256_cov_3.418818, whole genome shotgun sequence.
ACCESSION   DAOTOW010000724 DAOTOW010000000
VERSION     DAOTOW010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87720_length_1256_cov_3.418818"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..555)
                     /locus_tag="P5243_10805"
     CDS             complement(4..555)
                     /locus_tag="P5243_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRS19985.1"
                     /translation="MKTRIITFLAEGFEEIEAITPIDILRRAECEVITVSTTNSITVT
                     GAHGISIVADTLFSEMESFDADVIFLPGGMPGSLNLNAHEGVKHVVKQQAQKGKFIAA
                     ICAAPLILGGLGILQNKQATCYPGYENTLTGAEISKQSCVVDANIITGNGPGAAAKLG
                     FTLVSLLKGEAIANQWESGMMFS"
     gene            696..>1256
                     /locus_tag="P5243_10810"
     CDS             696..>1256
                     /locus_tag="P5243_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19986.1"
                     /translation="MNSEHLFLFEKIRSSKEIFQDAYTFIQQYAREYFVIVLLYAGPL
                     FVVSSYFASQGAVEIAQTGNFFINHNVWYSLIADFFADVVLNGITFGSIILYCKTGDI
                     KRDEVITFFNQNFWRILGITIISNIIISIGFMLLIIPGIVSLVPLTLFVYDKIYYNES
                     FEVSFIRSFELVKKNIKLSFGVVFIMY"
BASE COUNT          409 a          179 c          186 g          482 t
ORIGIN      
        1 cttttatgaa aacatcatcc cgctttccca ttggttagct atagcttctc ccttgagtaa
       61 tgatactaaa gtaaatccta attttgcagc agctcccgga ccatttcctg taataatatt
      121 agcatctaca acacaagatt gttttgaaat ttctgcacct gttagggtat tttcatatcc
      181 cggataacac gttgcttgtt tattttgtaa aatacctaat ccacctaaaa taagaggtgc
      241 tgcacaaatt gctgcaataa atttaccttt ttgagcttgt tgttttacaa catgtttaac
      301 tccttcatgt gcatttaaat tcagtgagcc gggcattcct cctggtaaaa aaataacatc
      361 ggcatcaaaa gattccattt cagaaaatag agtatcggca acaatactaa taccatgtgc
      421 tccggtaaca gttatggaat tggttgttga aacagttatt acttcacatt cagctctacg
      481 taaaatatct atgggagtaa ttgcttctat ttcttcgaac ccttctgcta aaaaagttat
      541 tattcttgtt ttcatctgtt tacaaatttg tatcaaaaat aataattttt acattcaaaa
      601 taaataatac tgaaactatc ttaataaatt gctaatacgt aaaaaacatg tacttttgta
      661 tttataacaa acgtaccaga aaaatattaa tataaatgaa tagtgaacat ttatttttat
      721 ttgaaaaaat acgaagtagt aaagaaattt ttcaagatgc atatacattt attcaacaat
      781 atgctcgtga atattttgta atagtattgc tatatgcagg tcctttattt gtagtttcat
      841 cttattttgc ttctcaaggt gctgttgaaa ttgctcaaac aggtaatttt tttataaatc
      901 acaatgtgtg gtattctctt atagccgatt tttttgctga tgttgttcta aatggtatta
      961 cgtttggcag tattatattg tattgtaaaa ccggagatat aaagcgagat gaggttataa
     1021 ccttttttaa tcagaatttt tggagaatat tgggtattac aataatttct aatataatta
     1081 tttcaatagg ttttatgttg ttaataattc cgggtattgt gagtttagtt cctttaacat
     1141 tatttgtata tgataaaata tactataatg aatcttttga ggtgagtttt attcgttcat
     1201 ttgaattagt gaaaaaaaat ataaaacttt cgtttggagt tgtatttata atgtat
//
LOCUS       DAOTOW010000725         1256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_87732_length_1256_cov_2.774355, whole genome shotgun sequence.
ACCESSION   DAOTOW010000725 DAOTOW010000000
VERSION     DAOTOW010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87732_length_1256_cov_2.774355"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="P5243_10815"
     CDS             complement(<1..275)
                     /locus_tag="P5243_10815"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656159.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRS19987.1"
                     /translation="MIYTLLHNATIVNRGEEFTGSILIHKQTIVDVFRDEVPETFLQK
                     NTRIIDCSGKYVIPGVIDDQVHFREPGLTHKGSIASESKAAIAGGITS"
     gene            complement(280..1029)
                     /locus_tag="P5243_10820"
     CDS             complement(280..1029)
                     /locus_tag="P5243_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRS19988.1"
                     /translation="MLLYVSDSIVIIPTYNEKENIENILRKVVSYPKGFHVLVVDDNS
                     PDGTARIVKKLQQEFPLQIHIIERQEKNGLGTAYIAGFNWALSKGYEYIFEMDADFSH
                     NPDDLIRLYDACNKGAHVAVGSRYIQGVNVVNWPMNRILLSYFASMYVRWVTCMVIRD
                     STAGFVCYKSAVLKTINLNTIKFKGYAFQIEMKFKAWKHGFTITEIPIIFTDRKEGTS
                     KMSKAIIKEAIFGVLQLRIQSWFTKYTKYTE"
BASE COUNT          398 a          220 c          174 g          464 t
ORIGIN      
        1 gaagttattc caccggcaat agctgcttta gattcacttg caatagaacc tttgtgtgtt
       61 aatccgggtt ctctaaaatg tacttgatca tcaattactc ccggtataac atatttgccg
      121 ctacaatcaa taattcgagt atttttttgc aaaaatgttt cgggtacttc atcacgaaat
      181 acatctacaa tagtttgctt atgaatgagg atacttccgg taaattcttc tcctcgattt
      241 actatagtag cgttatgtaa taaagtatat atcatgttat tattcagtat acttggtgta
      301 ttttgtaaac caactttgaa ttcgtaattg taaaacacca aaaattgctt cttttattat
      361 tgctttactc atctttgatg ttccttcttt acgatctgta aaaattatag gtatttcagt
      421 tatagtaaaa ccatgtttcc atgctttaaa tttcatttca atttgaaatg catatccttt
      481 aaatttaatt gtattaagat taatggtttt taaaacagca cttttgtaac atacaaatcc
      541 tgcagttgag tcgcgaatta ccatacatgt aacccaacga acatacatag aagcaaaata
      601 tgatagtaaa attctgttca ttggccaatt tacaacattt acaccttgaa tatatctgct
      661 tccaacagct acatgagctc ctttattaca agcatcatac aagcgaatca aatcatcagg
      721 attatgtgaa aaatcggcat ccatttcaaa tatatattca tatcctttgc taagagccca
      781 attaaaaccg gcaatgtatg ccgttcctaa tccatttttt tcttgtcttt caattatatg
      841 tatttgcaaa ggaaattctt gttgcaattt ttttacaata cgagctgttc cgtcaggaga
      901 attgtcgtca actaccaaaa catgaaatcc tttggggtat gaaacaacct ttcgaagaat
      961 attttcaata ttttcttttt cgttatacgt tggtattatt acaatgctat cagacacgta
     1021 aagtagtatt atgtatatac aaaaaaccct atcaaattat gtctgatagg gtttgatatt
     1081 ttttattaac gagctaattt aaactgtaca ggcaaattat accaaaccaa tactttttct
     1141 ccagcttgtt cacctggtgt aaatagtggt aatttattaa taacattcat tgcagctttg
     1201 tcaagaatag gtgctacaga tttaacaact tctggatttt ttgctttacc gtcttt
//
LOCUS       DAOTOW010000726         1256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_87747_length_1256_cov_2.474604, whole genome shotgun sequence.
ACCESSION   DAOTOW010000726 DAOTOW010000000
VERSION     DAOTOW010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87747_length_1256_cov_2.474604"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="P5243_10825"
     CDS             complement(<1..247)
                     /locus_tag="P5243_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19989.1"
                     /translation="MNTIVKINAKHLLLFVIIAVPFALFMNLYNSNVVTVRAEYPKQV
                     IAGTSFTVTVTIDKGNLSGFGRYTHTLPDGLRAESNSQ"
     gene            complement(463..>1256)
                     /locus_tag="P5243_10830"
     CDS             complement(463..>1256)
                     /locus_tag="P5243_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRS19990.1"
                     /translation="TTPKNSNYNLHIIKKSLYLIKLKTSTCMIENEELLRDAFKQKDW
                     QEIKANDTWQIFKIMSEFVEGFEKLARIGPCVSIFGSARTKPDNKYYALAEEIAFQLT
                     QHGYGIITGGGPGIMEAGNLGAKRGKGKSVGVNIDLPFEQEPNMFIDADKLINFDYFF
                     VRKVMFVKYSQGFVVMPGGFGTLDELFESLTLIQTMKIAHFPIVLVGKSYWTGLFDWV
                     KEQMLETEHNVSPDDLDLVKIVDTADEAVHIINDFYSRYILKPNF"
BASE COUNT          421 a          206 c          181 g          448 t
ORIGIN      
        1 tttgagaatt actttcagca cgtaatccat caggtaatgt atgagtatac ctaccaaatc
       61 cgcttaaatt acctttgtct atagttacgg taacagtaaa actggttcct gctatcactt
      121 gtttaggata ttctgcacgt acagtaacaa catttgaatt gtataaattc ataaataatg
      181 caaaaggcac agctataatt acaaacaaaa gcaaatgttt tgcattaatt ttaacaatag
      241 tattcataac acacttttat aaatatcgta attgcaaatg taatgcacga aaaataaaaa
      301 tgcaacaaaa atgcacaaaa ttgttaataa aaatgttaat aacgtgttag ctgataaaaa
      361 atatagtaat gttgtgatat aaaaaatgca tataacttac tgtatttcaa tactgcaaga
      421 tatatgcatt aattaagatt gcttgctgca aaaaaattgc gattagaaat taggtttaag
      481 tatatatcgt gaataaaagt cgttaattat atgaacagct tcgtcggctg tatctacaat
      541 ttttactaaa tctaaatcgt cggggcttac attgtgttct gtttcgagca tttgttcttt
      601 aacccagtca aataaacctg tccaatatga tttaccaact aaaactattg gaaaatgcgc
      661 aattttcata gtttgtatga gtgtgagtga ttcaaagagc tcgtctaatg taccaaatcc
      721 gccgggcatt acaacaaatc cttgtgaata ttttacaaac ataaccttgc gtacaaaaaa
      781 gtaatcaaaa ttaataagtt tatctgcatc aataaacata ttaggttctt gttcaaaggg
      841 taaatcaata tttactccaa cagattttcc tttacctctt tttgctccta aattacctgc
      901 ctccatgatt ccgggacctc cacctgttat gattccatat ccgtgttgtg tcagctgaaa
      961 tgcaatttct tcggctaaag cataatattt attatcgggt tttgttcgtg ccgaaccaaa
     1021 tattgataca catgggccta tacgtgctaa tttttcaaaa ccttctacaa attccgacat
     1081 tattttgaat atttgccagg tgtcgtttgc ttttatttct tgccaatctt tttgtttaaa
     1141 agcatcgcgc aataattctt cattttctat catacatgta cttgttttta atttaatcaa
     1201 atatagtgat ttttttataa tatgtaagtt gtaattactg ttttttggag ttgttg
//
LOCUS       DAOTOW010000727         1251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_88310_length_1251_cov_4.120401, whole genome shotgun sequence.
ACCESSION   DAOTOW010000727 DAOTOW010000000
VERSION     DAOTOW010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88310_length_1251_cov_4.120401"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="P5243_10835"
     CDS             complement(<1..548)
                     /locus_tag="P5243_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRS19991.1"
                     /translation="MKLDLGKPEKFSLLYSFLHNTWFRLLFFVWYKNITWTNIQRLYA
                     KYPIIIAANHQNSLMDPLVILGIETRQITWLARADIFKNPLAGRILRFMRIMPAFRQR
                     DGKEQLANNELVFNKCVDILAAKKVLAIFPEGTHWGYRRLRETKKAIPRIAFLTEEKF
                     NFTLNTHILPIGINYSNYTRLRG"
     gene            complement(695..>1251)
                     /locus_tag="P5243_10840"
     CDS             complement(695..>1251)
                     /locus_tag="P5243_10840"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HRS19992.1"
                     /translation="TVPLVYEARSWAHGTFIGSTVGSEVTAAAIGLAAGVRRDPFAML
                     PFCGYNMADYFGHWIKIGETAPDKTKLPKIFNVNWFRKDESGRWLWPGFGDNSRVLAW
                     IFDRCLGKAEAVETPIGFVPTIDGINIDGINVSKADMEELLKVDKNLWKAEVELIKEH
                     YARFGNKIPAALTAELKTLESKLA"
BASE COUNT          400 a          221 c          210 g          420 t
ORIGIN      
        1 cctctcaatc gggtatagtt ggagtaattt attcctatag gaagaatatg agtattgaga
       61 gtaaaattaa atttttcttc ggtaagaaaa gctattcgtg gtatagcttt ttttgtttcg
      121 cgtaatcgtc tgtatcccca atgggttcct tcggggaata tagcaagaac ttttttggca
      181 gctaaaatat caacacattt attaaacact aattcattgt ttgctaattg ttccttgcca
      241 tcacgttgtc taaaagcagg cattattctc ataaatctga gaattcgacc agctaaagga
      301 ttcttaaaaa tatcggcacg tgccagccat gtaatttggc gagtttctat gcctaaaatt
      361 actaaagggt ccattaatga attttgatgg ttagctgcaa ttataatagg atattttgca
      421 tatagccttt gtatgttagt ccacgttata tttttgtacc atacaaaaaa taataatcga
      481 aaccacgtat tgtgcaagaa actgtaaagt agtgagaatt tttccggttt tcctaaatca
      541 agtttcatag ttttattttt attgtaaaat gcaaacatag ttattttgcc tgaaactatg
      601 taagagtaat ggtattaaaa atgtagatat aaaaaaagag gtcgtttatt ttaaacgacc
      661 tctttacata ttatgattgc aaatctaaat gagattatgc taatttagat tctaatgttt
      721 ttaattctgc agtaagagca gccggtattt tgttgccaaa acgagcatag tgttctttaa
      781 ttaattcaac ttctgctttc caaaggtttt tgtcaacttt tagtaattct tccatatcag
      841 ctttagatac gttaatacca tcaatattaa taccgtcaat agtaggaaca aatccgattg
      901 gagtttcaac agcttctgct ttacctaaac aacggtcaaa aatccaagct aacacacgtg
      961 aattatcacc gaaacctggc cacaaccaac gtcctgattc atctttacgg aaccagttta
     1021 cattgaatat tttaggtaat ttagttttgt ctggtgctgt ttcgccaatt ttaatccaat
     1081 gaccaaagta atcagccatg ttgtatccgc agaatggtaa cattgcaaat gggtcacgac
     1141 gaacacctgc agctaaacca atagcagctg ccgtaacttc tgaacctaca gtagaaccaa
     1201 taaatgttcc atgagcccag ctacgagctt cgtatactaa tggtacagta c
//
LOCUS       DAOTOW010000728         1250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_88466_length_1250_cov_2.116318, whole genome shotgun sequence.
ACCESSION   DAOTOW010000728 DAOTOW010000000
VERSION     DAOTOW010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88466_length_1250_cov_2.116318"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..572
                     /locus_tag="P5243_10845"
     CDS             195..572
                     /locus_tag="P5243_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRS19993.1"
                     /translation="MKKSVQSQYAPKPIGPYSQAVVSGNTLYISGQLPYNAESEFLIT
                     TDISLATKQVMDNIGAILHAADMDFTHVVKCSIYVTDISKFAEVNAVYFNYFESPFPA
                     RETIQVAALPMGAMIEISAIAVK"
     gene            577..>1250
                     /locus_tag="P5243_10850"
     CDS             577..>1250
                     /locus_tag="P5243_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS19994.1"
                     /translation="MLHCTEDRYRGITIQIQNIPIEETRFTVDLQECIETYKHHPLLW
                     ITIPIEKSGLIPILTKQGFMFHNCKLNTLVLVKKNIQETHIPTTKNYIVGVGAIVIQA
                     NKILVVQNSFSHEFALPGGHIEKGETIQQALQREVLEETGIDIQFESIVNIGHFIQGQ
                     FGESNLYIVCTAKPLHNTISIQDTSEIVTAQWLAIHEFVESPYVNTYDKQVVKAAVTN
                     KDVKLC"
BASE COUNT          466 a          179 c          202 g          403 t
ORIGIN      
        1 cttttatatg ttttgtgttg cataccctta tacagcgata tagtacacaa ccaatagaaa
       61 ctattgaaaa ctcattgttt tttaaaccat tggctcattc ggcacaaggt ttattagaag
      121 gtaatattgt attaccttta ccagaacaaa atgtgttgca agaaattaaa tcaaactaaa
      181 tgatataaaa aaatatgaaa aaatcagtac aatctcagta tgctccaaaa ccaataggac
      241 catatagtca agctgtggta agtggcaata ccttgtatat ttcgggtcaa ttaccctata
      301 atgccgaaag tgaattttta attaccactg atattagcct tgcaacaaag caagttatgg
      361 ataatattgg agcaatttta catgctgccg acatggattt tactcatgtt gtaaagtgca
      421 gtatttatgt tactgatatt tcaaaatttg cagaagttaa tgctgtttat tttaattatt
      481 ttgagtcacc gtttcctgct cgtgaaacaa ttcaggttgc agcgttgcca atgggagcta
      541 tgatagaaat ttctgcaatt gctgttaagt aagcctatgt tacattgcac tgaagataga
      601 tatagaggta taacaattca aatacaaaat atacctatag aagagactcg ttttactgta
      661 gatttacagg aatgtataga aacatataaa catcatccgt tattatggat tactattcca
      721 attgaaaagt caggtttaat tccaatactt acaaaacagg ggtttatgtt tcataattgt
      781 aaattgaata cacttgtttt agtaaaaaaa aatatacaag aaacacatat accaacaact
      841 aaaaattata tagttggagt aggtgctatt gtaatacaag caaataaaat acttgtagtt
      901 caaaatagtt tttctcatga atttgcttta cccggagggc atatagaaaa aggagaaaca
      961 atacaacaag ccttgcaacg agaagtttta gaagaaaccg gaatagatat acaatttgaa
     1021 tcaattgtaa atattggaca ttttattcaa ggacagtttg gtgaatctaa tttatatata
     1081 gtatgtactg caaaaccatt acataataca atttctattc aagatacaag tgaaattgta
     1141 acagctcaat ggttggctat tcatgaattt gtagaatcac catatgtaaa tacctatgat
     1201 aaacaagtag tgaaagctgc tgttacaaat aaagatgtaa aactttgttt
//
LOCUS       DAOTOW010000729         1249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_88603_length_1249_cov_1.721943, whole genome shotgun sequence.
ACCESSION   DAOTOW010000729 DAOTOW010000000
VERSION     DAOTOW010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88603_length_1249_cov_1.721943"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="P5243_10855"
     CDS             complement(<1..1182)
                     /locus_tag="P5243_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19995.1"
                     /translation="MNKKTDMFYKKMGVIRKRIIGDMKTQLKYSKVGIQLKIFLVCAM
                     SVLFVETIQSANITAGTVNYNVSATSAGGTAYTSADNLTCQGWSSPTYTLTEDITVVN
                     LTISESVTLNLNNFNLTVTGTLLISENGTISAVESSGTKSITASTFSFGGNTNLNTTG
                     YDYNITNAYSNSSRTLSTNGGNFTVSSGGISLSSSNAWINTGGGNLSISGDLQLSNAA
                     YINVGAGNATIGGNYTIGDWQGNAHIAFTNGNLTIQGAISIGSKNGGEESIKCTGTGW
                     VIFNGSSLTANNNSSLGTIPRFRQSSSSFTKSGSGTVTISTTFDRNCGAAPTVSAGAF
                     TISGSTINAPTTYYSCNGADLSNVANWWTNTNCTGSNPSDFTNACSIFEVQSGHSCTF
                     GT"
BASE COUNT          400 a          246 c          189 g          414 t
ORIGIN      
        1 agttccaaac gtacaactat gtccactttg cacttcgaat atactacaag catttgtaaa
       61 atcagaagga ttagaacctg tacaattggt atttgtccac cagtttgcaa cattcgataa
      121 atcggcacca ttacatgaat aatatgtagt tggagcattt atagttgaac cgcttatagt
      181 gaaagccccc gcactcacag taggtgcagc accacaattt ctatcgaacg tagttgaaat
      241 ggtaaccgta ccactaccac ttttagtaaa cgaagaactt gattgcctaa atcggggaat
      301 ggttcctagt gaactattat tattagcagt taatgatgaa ccattaaaaa taacccaacc
      361 tgttccggta catttaatag attcctcacc tccattttta ctacctatcg atattgcccc
      421 ttgaattgtt aaattaccat tggtaaatgc aatatgagca tttccctgcc aatcaccaat
      481 tgtataattg ccacctatag ttgcatttcc tgctcctaca ttaatatagg cagcattaga
      541 caattgcaaa tcacccgaaa tactaagatt tccgccaccc gtattaatcc acgcattact
      601 tgaagataac gaaataccgc ccgaagaaac agtaaaattt ccaccattgg ttgaaagcgt
      661 acgtgaagaa tttgaataag catttgttat attataatca tatccggtag tatttaaatt
      721 cgtattaccg ccaaacgaaa acgtagaagc agtaatagat tttgtacccg aactttcaac
      781 agccgatata gtaccatttt cacttattaa taatgtgcca gtaacagtaa gattaaaatt
      841 attgagattt aaagttacac tttccgaaat ggtaagattt accacagtta tatcttcagt
      901 taaggtatat gttggactac tccagccctg acaggtaaga ttatcggctg acgtatatgc
      961 tgtaccaccg gccgaagttg cagaaacatt ataattgaca gttcctgcag taatatttgc
     1021 actttgtatt gtttcaacaa acaagacact catagcacat actaaaaata tttttagctg
     1081 tatgccgact tttgaatatt ttagttgtgt tttcatatct cctataatac gttttcgtat
     1141 tacccccatt tttttgtaaa acatgtctgt ttttttattc atgttatatt ctatatttta
     1201 ttactattta ttttattata cgatattgta gtgcattcaa gtttcactt
//
LOCUS       DAOTOW010000730         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_88688_length_1248_cov_2.276614, whole genome shotgun sequence.
ACCESSION   DAOTOW010000730 DAOTOW010000000
VERSION     DAOTOW010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88688_length_1248_cov_2.276614"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1248
                     /locus_tag="P5243_10860"
     CDS             <1..>1248
                     /locus_tag="P5243_10860"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19996.1"
                     /translation="SGSYVIKPIPAAPAVTSESICVNEPNVALQATGSTIKWYQNANR
                     TGYIQTSQGYTPAVTSDNTYTFHATQTVDGCESQTAPATYEVKPLPAAPIVSVTPNSI
                     CEYNTDPQFMATAVNGATVKWYKDDESTFIQNGASLTPVRPVPAQYPLTIKYFASQVV
                     NGCEGPKNSATLQIKAKPKLPIASDKAVCEGQTTSLPAVSTQNATLNDKWYDDAAATS
                     LLGTGYSYQVQPGEFSNADRNYYVVRVIQGCSSDTVPVKLAVIKKPTFTIGNDIEECA
                     ANAKAIVTASGFNPAINANSTITWGVNSKGLDDNVQHAVVPASKLIPINTDANSVNKV
                     YAIYRYKDASGITCISDTVSIKYTLHKKARTPIVAFNTICQGAEIQPLQAFGSPNIVW
                     ESKNGLPTSYGQTYNFSQLGITE"
BASE COUNT          462 a          211 c          235 g          340 t
ORIGIN      
        1 taagtggtag ttatgtaatt aaaccaatac cggcagcacc tgcagtaaca agtgagtcaa
       61 tctgtgtgaa cgaaccaaat gttgcattac aagcaaccgg tagtaccata aaatggtatc
      121 aaaatgcaaa caggacagga tatatacaaa caagtcaagg gtatacaccg gcagtaacaa
      181 gcgacaatac ctatacattc catgcaacac aaacagtaga tggttgtgag agtcaaacag
      241 cacctgcaac gtatgaagta aaaccgttgc ctgcagcacc aattgtaagt gttacaccta
      301 atagtatatg tgagtacaat actgatccac agtttatggc tactgctgtt aatggagcaa
      361 ctgtaaaatg gtacaaagat gatgaaagta cgtttattca aaatggtgca agtttaacac
      421 ctgtacgtcc cgtacctgct caatatccat taacaattaa atactttgca tcgcaagtag
      481 taaatggttg tgaaggacct aaaaatagtg caacattaca aattaaggct aagcctaaat
      541 taccaatagc tagtgataaa gctgtgtgcg aaggtcaaac aacaagttta cctgcagtat
      601 caacacaaaa tgcaacattg aatgataaat ggtatgatga tgctgctgct acaagtttac
      661 ttggtaccgg ttattcatat caggtacaac cgggagaatt ttcaaatgct gatagaaatt
      721 attatgtggt tcgtgtaata caaggatgta gcagtgatac tgtacctgtt aagttagcgg
      781 taattaaaaa gccaacattt actataggta atgatattga agaatgtgct gcaaatgcaa
      841 aagctatagt aacagcaagt ggatttaatc ctgctatcaa tgcaaattct acaattacat
      901 ggggagtaaa ttcaaaagga ttagatgata atgttcaaca tgcagtagtt cctgcaagca
      961 agttaattcc aataaatacc gatgcaaata gtgtaaataa agtatatgct atttatcgtt
     1021 acaaagatgc ttctggtata acatgtataa gtgatactgt atcaattaaa tatacattgc
     1081 ataaaaaagc acgtacaccg atagtagcat ttaatactat ttgtcaaggt gcagaaattc
     1141 aaccacttca agcatttggt agcccaaata ttgtatggga aagtaaaaat ggtttaccta
     1201 ctagttatgg tcaaacatac aatttctcac aacttggaat tacagaac
//
LOCUS       DAOTOW010000731         1243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_89175_length_1243_cov_3.456229, whole genome shotgun sequence.
ACCESSION   DAOTOW010000731 DAOTOW010000000
VERSION     DAOTOW010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89175_length_1243_cov_3.456229"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="P5243_10865"
     CDS             complement(<1..773)
                     /locus_tag="P5243_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19997.1"
                     /translation="MTNSLEKTNYPALYTLIIVFFFWGFIAAGNSIFIPFCKHYFSLD
                     QFQSQLIDFAFYTAYYIGALVLFAYSSLRGTDLVNSWGYKKSIVYGLLFSALGAGAMI
                     LAVFSNIYIGMLTGLFIVALGFSLQQTAANPFAVLLGDPKTGSSRINLGGGVNSFGTT
                     IGPLIVAFALFGSVSEISDEQIAGLQLNKVILLYICVGILFVTAAAIFHFSKKVPAGI
                     QDEHVEKAHKALRSLFIITGLLLCMFVPVFHSYAQGWEAP"
     gene            882..>1243
                     /locus_tag="P5243_10870"
     CDS             882..>1243
                     /locus_tag="P5243_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19998.1"
                     /translation="MRKLIYSLFLFFSVVTIQAQDIIHLNSGSQIKAKVLEIKPNIIT
                     YKLYENIQGPTYSIYLQEVHSIAFENGYVETISHTAAPQQLNQTSVGNQSKAAPLICN
                     KGTVKQNNVELKDEEILK"
BASE COUNT          473 a          209 c          174 g          387 t
ORIGIN      
        1 ggtgcttccc aaccttgagc atatgaatgg aatacaggca caaacataca cagtaataaa
       61 ccggtaatta tgaataatga acgtaatgct ttgtgtgcct tttccacatg ctcatcttgt
      121 ataccggcag gaactttttt agaaaaatga aatattgctg ctgctgttac aaacaatatt
      181 cctacacata tatacaggag tattactttg ttcaattgca atccggctat ttgttcatcg
      241 cttatttcag aaacactgcc aaataatgca aatgctacaa taagaggccc tatggtggta
      301 ccaaatgaat tcactccgcc tcctaagttt attctgctgc ttccggtttt ggggtcgcca
      361 agtaatactg caaaaggatt ggcagctgtt tgttgaagcg aaaaccctaa agcaactata
      421 aataaaccgg taagcattcc tatataaata ttagaaaata cagcaagaat cattgctcct
      481 gctcctaatg cagaaaataa taatccatat acaatacttt ttttgtaacc ccatgaattt
      541 accaaatcgg ttccacgtaa agaactatag gcaaatagta ctaaagcacc aatataatat
      601 gccgtgtaaa acgcaaaatc aattaattgg gattgaaact gatctaatga aaaataatgt
      661 ttacaaaacg gaataaaaat actattaccg gctgctataa atccccaaaa aaagaataca
      721 attattaatg tatacaatgc aggataattt gttttttcta aagagtttgt catatatact
      781 tagttttata caaaataacc aaaaaaaaat tacttttcat gcattctatt ctcatgtttt
      841 ttaagtagtt ttgaatatat tttactttta aaatcaagaa aatgagaaaa ttaatctata
      901 gtttattttt attttttagt gttgtaacaa ttcaagctca agatataatt catctaaatt
      961 caggctctca aataaaagca aaagttcttg agataaaacc caatataata acctataaat
     1021 tatatgaaaa tattcaaggg ccaacatatt ctatatattt acaagaggtg cattctatag
     1081 cttttgaaaa cggatatgta gaaactattt cacatacagc agcccctcaa caattaaatc
     1141 aaacttctgt aggtaatcaa tctaaagcag ctccgcttat ttgtaataaa ggaacagtaa
     1201 aacaaaataa tgttgaatta aaagacgaag aaattttaaa att
//
LOCUS       DAOTOW010000732         1243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_89199_length_1243_cov_2.548822, whole genome shotgun sequence.
ACCESSION   DAOTOW010000732 DAOTOW010000000
VERSION     DAOTOW010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89199_length_1243_cov_2.548822"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="P5243_10875"
     CDS             <1..413
                     /locus_tag="P5243_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS19999.1"
                     /translation="IRQFNDSLYQFISYVVYKKTIQDFFENYLYQGKNNLDSLIQVIV
                     HNISICDSVVIELQAYTGKGGNYEYNVQLAQRRIASTKEYIINECAKFEISKDRIHFI
                     IVPEIQKVGKQEYNTLFEIDDALHRKVIIRLKEI"
     gene            complement(399..>1243)
                     /locus_tag="P5243_10880"
     CDS             complement(399..>1243)
                     /locus_tag="P5243_10880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRS20000.1"
                     /translation="EGLSPIIKHLKYIQTITNIIVVAIENKRLYKESLEKEAYKKELE
                     VASRMQAMLIPSFDALPKNNYLNVAAYYRPHSQVGGDYYDFISLNNQEFGFCIADVSG
                     KGVSAAILMSNFQANVRALFKSNISLIQLIKELNQHVNESAHGEKFITFFIGKYNVRD
                     RILHYINAGHNPPLLLKKKENTMLYLTNGCVGLGMFDDIPTIKVGTVHIDSGDKMVCY
                     TDGLVEAENEDYNKFGTFPLEGALSIDGNIEETIQYLNDKLEQFLHGKTLGDDISIIG
                     LDFF"
BASE COUNT          465 a          193 c          161 g          424 t
ORIGIN      
        1 atattcgaca attcaacgac agtttatatc aatttatctc atatgtagta tataaaaaga
       61 ctatacaaga tttttttgag aattaccttt atcaaggaaa aaacaattta gattcactta
      121 tacaagtaat tgtacataat atttctatat gtgacagtgt tgttatagaa cttcaagctt
      181 ataccggtaa aggaggaaac tatgaatata atgttcagtt agctcaaaga agaattgcat
      241 cgacaaaaga atatattata aacgaatgtg ctaaatttga aatatcaaaa gataggattc
      301 attttataat tgtacccgaa atacaaaaag taggaaaaca agaatataat actctttttg
      361 aaattgatga tgctttacat cgaaaagtaa taattcgttt aaaagaaatc taacccaata
      421 atggaaatat catcacctaa tgttttacca tgtaaaaatt gttctaattt atcatttaaa
      481 tattgaattg tttcttcaat attcccatca atagataaag ctccctcaag aggaaaagtt
      541 ccaaatttat tataatcttc attttcggct tcaactaacc catcggtata gcaaaccatt
      601 ttatcaccag aatcaatatg cacagttcca actttaattg ttggaatatc atcaaacatt
      661 cctaatccaa cacacccatt ggtaagatac aacatggtat tttctttctt tttgagtaat
      721 aatggtggat tatgtccggc atttatataa tgtaaaattc tatctcgaac attgtatttt
      781 ccaataaaga aggtaataaa tttttcaccg tgagcactct cattcacatg ttgattcaat
      841 tccttaatta actgaattaa agaaatatta cttttaaaaa gagctctgac attagcttga
      901 aaatttgaca ttaaaattgc agcagacaca ccttttccgg aaacatctgc aatacaaaaa
      961 ccaaattctt gattatttaa tgaaataaag tcataataat cacctcctac ttgtgaatga
     1021 ggacgataat atgcagcaac atttaaatag ttatttttgg gcaaagcatc aaacgaagga
     1081 attagcatgg cttgcatacg tgaagcaact tcaagctctt ttttatatgc ttctttttca
     1141 agagactctt tatataatcg tttattttca atagcaacta caataatatt agtaattgtt
     1201 tgaatatatt ttaaatgttt tataataggg ctcagacctt cgc
//
LOCUS       DAOTOW010000733         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_89289_length_1242_cov_5.285594, whole genome shotgun sequence.
ACCESSION   DAOTOW010000733 DAOTOW010000000
VERSION     DAOTOW010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89289_length_1242_cov_5.285594"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="P5243_10885"
     CDS             complement(<1..445)
                     /locus_tag="P5243_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20001.1"
                     /translation="MKRIGVIVVFVLLQVFLFAQTNENKQNIELLDKLLQLDKPPITP
                     TLSFPIVTIGSGIFRYHGDLPLQTPPNFTIGTPGMHISLSQPLNEKFSLDLFAMKGSL
                     IGSYYPTKTNTALNFKTDLTSFGTSVHYNFGQIQKYSSPNNKILSP"
     gene            complement(494..>1242)
                     /locus_tag="P5243_10890"
     CDS             complement(494..>1242)
                     /locus_tag="P5243_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20002.1"
                     /translation="TAQPYQQQGATAQTTTRVPTQTVSGTTPTAQQVQVQNGVVTTKP
                     MQTTAAGQQVQPTTNGITAQPYQQQGATAQTTTRVPTQTVTGTAPTTQQVQIQNGVVT
                     TKPMQQTIIKDENHNNLLVDNTATPQARLSNKDNFSQNQTKITQIPEPNRKLDVYFRV
                     QISAGHKLVNTKYYFAKLNIRDNIIVEQIEGWFKYTINKFDTYVNARNQRNTIWNESP
                     VKDAFVVAYNGRNRITVQEALMITNQKWVQ"
BASE COUNT          324 a          169 c          270 g          479 t
ORIGIN      
        1 atggtgaaag aattttgttg tttggtgaag aatatttttg tatttgtcca aaattatagt
       61 gcacacttgt tccaaatgat gtaaggtctg ttttaaaatt taatgctgta ttagttttgg
      121 ttggataata cgaaccgata agtgaacctt tcatagcaaa caaatctaaa ctaaattttt
      181 cattcaatgg ttgtgataga gatatgtgca ttcccggtgt accaatagta aaatttggtg
      241 gcgtttgtaa tggtaaatca ccatgataac ggaaaattcc tgaaccaatt gttactatgg
      301 ggaatgaaag ggttggggta atgggaggtt tatctaattg aagaagtttg tcaagtaact
      361 caatattttg tttgttttcg tttgtttgag caaacaaaaa tacctgcaaa agtacaaata
      421 ctactatgac tccaattctt ttcattacag tatattttat atgcaggttg tattaaaaca
      481 atgctacata tggttactga acccattttt gatttgttat cataagagct tcttgtacgg
      541 taattctgtt tcgtccattg tatgctacca caaatgcatc ttttaccggt gattcattcc
      601 aaatggtatt tctttgatta cgggcattta cataggtgtc gaatttatta atggtgtatt
      661 taaaccatcc ctcaatttgc tctacaataa tgttatctct gatgttcaat tttgcaaaat
      721 aatattttgt gttaaccaat ttatgtcctg cagatatctg cactcggaaa tatacgtcga
      781 gtttgcgatt tggttccggt atttgtgtaa tttttgtttg attttgtgaa aagttgtctt
      841 tattggatag tcgagcctga ggagttgcag tattgtctac taagagatta ttatgatttt
      901 catcttttat tatagtttgt tgcataggtt tagtagtaac aacaccgttt tgtatttgta
      961 cttgttgtgt agtaggagcg gtgccggtaa ctgtttgtgt aggtacacgt gtggtagttt
     1021 gtgcggtagc tccttgttgt tgatatggtt gagcagtaat tccgttagtg gttggttgta
     1081 cttgttgacc tgcggcagta gtttgcatag gtttagtagt aacaacaccg ttttgtactt
     1141 gtacttgttg tgcagtggga gtagttccgc ttaccgtttg tgtaggcaca cgtgtggtag
     1201 tttgtgcggt agctccttgt tgttgatatg gttgagcagt aa
//
LOCUS       DAOTOW010000734         1230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_90671_length_1230_cov_3.192340, whole genome shotgun sequence.
ACCESSION   DAOTOW010000734 DAOTOW010000000
VERSION     DAOTOW010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90671_length_1230_cov_3.192340"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1230)
                     /locus_tag="P5243_10895"
     CDS             complement(<1..>1230)
                     /locus_tag="P5243_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20003.1"
                     /translation="ISPAIQNAQDIVEWKINPGNAIIKSNAPVSLAGYITTADIRTDI
                     NYTITARYQVKLATATCYSNIDTLIYTVHKRAHTPIVFSNVICQGQSLEPLKALGTPN
                     LTWTSNHPLIPSPVKGREYNFAQQGITTLPEGTYSFELIDESEFGCKSLIATPSITVA
                     PAARTKIIGDSTVCEYTIEQLYTLEYVPQQPSSYKWEVTGKHVNYTKDANASNIRYVD
                     WGKAGIDTLRVYERTFYGCDGYDELIVNIAEYPIAKFTYTMPGAVDVVWFSDSTIQKP
                     IVEITDTGETVTIPISYTMYWNFDQLGETYYDRIVPYSQRKEIVEATGYTYGYKNPEL
                     MVENEYGCRSTYSKEINININAAIFVPTAFSPTNAAYSVHYFQPVGYNIKTCEIWIYD
                     LWGNLVWYSNEVENGIFV"
BASE COUNT          378 a          227 c          201 g          424 t
ORIGIN      
        1 acaaatatac cattttcaac ttcgttacta taccacacca aattacccca caaatcgtat
       61 atccatatct cgcaagtttt aatattgtat cctaccggtt gaaaataatg aacactgtat
      121 gctgcattgg taggagaaaa tgctgttgga acaaaaatag cagcatttat attaatattt
      181 atttctttag aatatgttga acgacacccg tattcatttt caaccatcaa ttcgggattt
      241 ttgtagccat aggtatatcc ggtagcttct acaatttctt tccgttgtga atatggcaca
      301 attcggtcat aatatgtttc tcctaactgg tcaaaattcc aatacatggt atatgaaata
      361 ggaatagtta cagtttcacc ggtatcagta atttcaacta ttggtttttg aatagtacta
      421 tcgctaaacc aaaccacatc aacagcaccg ggcatagtat aagtaaattt agcaatagga
      481 tattctgcta tattcacaat taattcatca tatccgtcgc acccataaaa tgtacgttca
      541 tatacacgta acgtatctat tcctgcttta ccccaatcaa cataacgaat attactagca
      601 tttgcatctt tggtataatt tacgtgctta cctgtaactt cccatttata cgaacttggt
      661 tgttgaggta cgtattctag tgtatacagt tgttcaattg tatattcaca tacggtagag
      721 tctccaataa tttttgttcg ggctgccgga gcaacagtta tagaaggtgt tgcaattaaa
      781 cttttacaac caaattctga ctcatcaatt aattcaaaac tatacgttcc ttcaggaagt
      841 gttgtaatac cttgttgtgc gaaattatat tctcgtcctt ttacaggaga aggtataagc
      901 ggatgattag atgtccacgt taaatttggt gttcctaatg cttttaatgg ttcaagagat
      961 tgaccttgac aaataacatt tgaaaaaaca attggagtat gagctcgctt atgcacagta
     1021 tatataagtg tatcaatatt gctataacat gttgctgttg ccaattttac ctgatatcgg
     1081 gctgtaattg tataattgat atcggtacgt atatcggcag ttgtaatata tcctgcaagc
     1141 gacaccggag cattgctttt tataattgca ttacccggat tgattttcca ttcaacaata
     1201 tcttgagcat tttgtattgc cggacttatg
//
LOCUS       DAOTOW010000735         1227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_91070_length_1227_cov_1.869454, whole genome shotgun sequence.
ACCESSION   DAOTOW010000735 DAOTOW010000000
VERSION     DAOTOW010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91070_length_1227_cov_1.869454"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..>1227
                     /locus_tag="P5243_10900"
     CDS             400..>1227
                     /locus_tag="P5243_10900"
                     /inference="COORDINATES: protein motif:HMM:NF036509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRS20004.1"
                     /translation="MTKKNKGYTYANALPIPTEIIQLLRKNPRKPYNVKEIATKLGIN
                     NKEGKHAVEFHLEKLLQQNEIEETQHGKYRLITKGGYIIGKVDLTAYGTAYVISPESK
                     EDIFISSSNLNHALNGDTVKVYLFAKNPNRRPEGEVIEILEQGKREFVGIIEKNKHYA
                     FVIVDKKQIPFDIFVAQEHTKGAKDGQKVIVRITEWPRKAKNPIGEVIEVLGDVGNNE
                     TEMHAILAEFDLPYRFSKEVEDFAATLPEEISEKEIAKRQDFRPITTFTIDPEDAKDF
                     "
BASE COUNT          482 a          180 c          179 g          386 t
ORIGIN      
        1 tgggtacaat ccgtttgttg tttttactag tgtcatatct tctgcactcc tttcttttta
       61 tattttttat ttttttaatt ttcattttct gtggtgggtg ttttcaaaat aaataccgaa
      121 attatatttt tttataaaaa tctaaaaatc aatttgttaa caatattttt ataaaatgac
      181 atattgtcgt taaaaagcaa tttgtagtgc catattggca atattaaaaa tatttaaaat
      241 atattaacaa ttaaaaatca aatagttaca aaaaaattaa ctatatatta ttttatgcca
      301 ttattgtaaa caatatttct taataaaata ttacaaaagc atactacaat actcatgaat
      361 attttgtatt attgttacta tagatttaaa cgtttaccta tgacaaaaaa aaacaaagga
      421 tatacttatg ctaatgcatt gccaattcca acagaaatta ttcaattatt acgaaaaaat
      481 cctcgtaaac catacaatgt aaaggaaatt gcaacaaagt tgggaataaa caataaagaa
      541 ggcaaacatg ctgttgaatt ccatttggaa aaactattgc aacaaaatga aatagaagaa
      601 actcagcatg gaaaatatcg tttaattact aaaggtggtt atattatagg aaaggtagat
      661 ttaactgcat acggcactgc atatgttata agcccggaat ctaaagaaga catctttatt
      721 tcatcatcaa atcttaatca tgcactcaat ggcgataccg taaaagtgta tttgtttgca
      781 aagaatccaa atcgcagacc cgaaggtgaa gtaattgaaa tacttgaaca aggtaaacga
      841 gaatttgtgg gtattataga aaaaaataaa cactacgcat ttgtaattgt tgacaaaaaa
      901 caaataccat tcgatatttt tgttgctcaa gaacatacca aaggggcaaa agacggacaa
      961 aaagttatag ttcgcataac cgaatggcca cgcaaagcaa aaaatcctat aggtgaagtc
     1021 atagaagttt taggtgatgt aggtaacaat gaaaccgaaa tgcatgctat acttgcagaa
     1081 tttgacctcc cctatcgctt ttcaaaagaa gttgaagatt ttgctgctac attacccgaa
     1141 gaaattagtg aaaaagaaat tgcaaaacgc caagacttta gacctattac aacctttact
     1201 attgaccccg aagacgcaaa agatttt
//
LOCUS       DAOTOW010000736         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_91381_length_1224_cov_2.361848, whole genome shotgun sequence.
ACCESSION   DAOTOW010000736 DAOTOW010000000
VERSION     DAOTOW010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91381_length_1224_cov_2.361848"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="P5243_10905"
     CDS             <1..542
                     /locus_tag="P5243_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20005.1"
                     /translation="QLPIQQLDKQKIAYVSLQNQDVDTFLLAAKQYTSITPFVIPHNT
                     EYSATLTNLINDLRSFTTVIVNVVGSSQFPSKKYGITQGMIDIVSKIAKEHNVILVLH
                     ANPYALDYFKDAVPSISSIVIGYDFSKMVQYETPQVLFGALPAQGLLPVGVGGLPAGT
                     GKLYRSIDRLRYATPLKQE"
     gene            539..>1224
                     /locus_tag="P5243_10910"
     CDS             539..>1224
                     /locus_tag="P5243_10910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRS20006.1"
                     /translation="MNEIKLQIIDSIVNAAIAAKATPGCQVLVAKNGIVVFNKAYGTY
                     SYDSKQQVTPETLYDLASLTKVAATTISLMKLYDQKLFSLDATLGTYIPQARGTNKDT
                     LRMSNILTHQARLHPWIPFYQYLIQDFFNPKVKDIKNNKRTPIYSVKINNNLYVNKNY
                     GFIDSSISSVKSDLYSLQIADNVYMNPAKRDLVYSIIYNSKLNDKKEFVYSDLGFILF
                     ADVIKNLTGR"
BASE COUNT          436 a          171 c          197 g          420 t
ORIGIN      
        1 atcaattacc tattcaacag ttagataaac aaaaaatagc atatgttagt ttgcaaaatc
       61 aagatgttga tactttttta ttagcggcaa aacaatatac ttctattacc ccttttgtaa
      121 taccacataa tacggaatat agtgctactc ttactaatct tatcaatgat ttgcgttcat
      181 ttactactgt tattgtaaat gtagtaggca gtagtcaatt tccatcaaaa aagtatggta
      241 taacgcaggg aatgatagat atagtttcta aaattgcaaa agaacataat gtaatacttg
      301 tgttacatgc caatccgtat gctttagatt attttaaaga tgctgtgccg tcaatttctt
      361 ctatagtaat tggttatgat tttagtaaaa tggtgcagta tgaaactcct caagtattat
      421 ttggtgcttt acctgctcaa ggattattac ctgttggtgt aggagggtta ccggcaggta
      481 ccggaaaatt gtatcgttca attgataggt tacgctatgc tactcctttg aagcaggaat
      541 gaacgaaata aaattacaaa taatagattc aattgtaaat gctgcaatag ctgctaaagc
      601 aacccccggt tgtcaagtgt tagtggcaaa gaatggaatt gttgtattta ataaagcata
      661 tggtacatat tcttacgatt ctaaacaaca agtaacacca gaaacattgt atgatttagc
      721 atcgttaacc aaagttgctg caactactat aagtttaatg aaattgtatg atcaaaagtt
      781 attctctctt gatgcaacat tgggaaccta tattccgcaa gcaagaggta ctaataaaga
      841 tacattgcga atgagtaata ttttaacaca tcaagctcgt ttgcatcctt ggattccatt
      901 ttatcaatat ttaatacaag atttttttaa tccaaaggta aaggatatta aaaataataa
      961 gcgtacacct atttacagtg taaagattaa taataattta tatgtaaata aaaattatgg
     1021 gtttattgat agttcaatat cttcggttaa atctgattta tattctcttc aaattgccga
     1081 taatgtgtat atgaatcccg caaaacgaga tttggtatat tcaattattt ataattctaa
     1141 attaaacgat aaaaaagaat ttgtatatag tgatttaggt tttatattat ttgccgatgt
     1201 aataaaaaat ctaacaggta gaaa
//
LOCUS       DAOTOW010000737         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_91737_length_1221_cov_2.451115, whole genome shotgun sequence.
ACCESSION   DAOTOW010000737 DAOTOW010000000
VERSION     DAOTOW010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91737_length_1221_cov_2.451115"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1221)
                     /locus_tag="P5243_10915"
     CDS             complement(<1..>1221)
                     /locus_tag="P5243_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20007.1"
                     /translation="VVSNYFEGTQKSTKIGPSNHFQEYVWNITTVPGEIATYTLKAIS
                     QQQDIYSAIKQSSNNHDRLDTLINAKLFPYLELENYTQDNLLRTPPHLNYWKVYYDPV
                     GEIAVTPKHIFTLYNDTLQQGDTLRLNVSAQNVAYVNMDSVLILYEIRSVTNELIVAK
                     YQRVAPALAQQYIYDQLSYSTKELSGTYTIRIEFNPINPNTGEYDQLENSRINNFLYT
                     HFYVKTDKTKPILDVTIDNRHVSNGDYISSKPTILLRLYDENQYLLLQDTSVFSVKIT
                     NVLSGITTTYYFNGSNLRFVPATANNKTCYAIFSPEFTENGTYILSIEAKDMSNNLSG
                     IEPYTIEFTIDLESKISVLYNFPNPCTDATTFRFILTGSKIPETMYIHIYANDGKLVH
                     TIPVHTAKHIHIGT"
BASE COUNT          385 a          167 c          222 g          447 t
ORIGIN      
        1 ttgtccctat atgaatatgt tttgctgtgt gtaccggaat agtatgaact aatttaccgt
       61 catttgcata tatatgtatg tacatagttt cgggtatttt actgcccgta agtataaacc
      121 gaaatgtagt agcatcggta caagggttag gaaaattgta taataccgaa atttttgatt
      181 ctaaatcaat tgtaaattct atggtatacg gttcaattcc cgatagatta ttactcatat
      241 ctttagcttc tatactcaat atatacgttc cattttcagt aaattcggga gaaaatatgg
      301 catagcacgt tttgttattg gccgtagcag gaacaaatcg taaattacta ccattaaagt
      361 aatatgttgt agtaatgcct gaaagaacat tggttatttt tacagaaaac acacttgtat
      421 cttgtagcag taaatattgg ttttcgtcgt ataatcttaa taatatggtt ggtttactcg
      481 aaatataatc gccattcgat acatgtctgt tatcaatggt aacatctaaa attggtttag
      541 ttttatcggt ttttacatag aaatgagtat acaaaaagtt attaattcta ctattttcta
      601 attggtcata ttcgcctgta tttggattga ttggattaaa ttcaatacga atagtataag
      661 taccgcttaa ttctttagtg ctatagctaa gttggtcata tatatattgc tgagccaaag
      721 ccggagctac acgttgatat ttggcaacta ttaattcgtt agtgacagaa cgaatttcat
      781 atagtataag aacactatcc atgttgacgt aagctacatt ttgtgcagaa acgtttaatc
      841 gtaatgtgtc accttgttgt aaggtgtcgt tatagagagt aaatatatgt tttggtgtaa
      901 cagcaatttc gccaacaggg tcataataaa ctttccaata attgagatga ggaggtgtac
      961 gcaataaatt atcttgcgtg taattttcta attccaaata tggaaataat tttgcattta
     1021 taagagtatc gagtctgtca tgattgttag aagattgttt aattgcacta tatatatctt
     1081 gttgttgcga tatagcttta agtgtatatg tagcaatttc tccgggaacg gtagtaatgt
     1141 tccaaacata ttcttgaaag tggtttgaag gaccaatttt tgtagatttt tgggttcctt
     1201 caaaataatt ggaaacaaca t
//
LOCUS       DAOTOW010000738         1216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_92316_length_1216_cov_2.513351, whole genome shotgun sequence.
ACCESSION   DAOTOW010000738 DAOTOW010000000
VERSION     DAOTOW010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92316_length_1216_cov_2.513351"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1099
                     /gene="murB"
                     /locus_tag="P5243_10920"
     CDS             86..1099
                     /gene="murB"
                     /locus_tag="P5243_10920"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRS20008.1"
                     /translation="MIVQSENVSLQKYNTFGIDVYASYFFSYTHIDELQEYLKRDFKA
                     ESSFFILGGGSNVLFLDNYKGTIIHPNNKGISIIAQDDTTVRVKVGAGEIWDDFVAWA
                     VTHELYGIENLSLIPGSVGAAAVQNIGAYGVEVGSVIFEVHTIDMFTLQTHKFSHKQC
                     AFEYRNSIFKQAHMKHHIITDVCFELNKKGSCSVSYGSLQQTVLSLGEISLHNVRKAV
                     IEVRNQKLPNPNEIGNAGSFFTNPIVSKDVFNKIQQKYPDIVWYAAPDNMVKIAAGWL
                     IDKLGLKGFSLGLAQVHSKQALVLINKGGASGRDIQKLAEYIQDKVFNETGIELKPEV
                     IYI"
BASE COUNT          410 a          145 c          219 g          442 t
ORIGIN      
        1 atcattgctt acggcaagta ttatgtgttt tttttctctg ttcatgcatt tttttctgta
       61 ttttcgctta ttaaattatt agtatatgat agttcaaagc gaaaatgttt cgttacaaaa
      121 gtacaataca tttggtatag atgtatatgc ttcatatttt ttttcttata ctcatataga
      181 tgagttacaa gaatatctta aacgtgattt taaagctgag agttcttttt ttatcttggg
      241 aggaggaagc aatgtgctat ttcttgataa ttataaagga actattattc atcctaataa
      301 taagggaatt tcaattatag ctcaagatga tactacagta agagtaaaag taggagcagg
      361 cgaaatttgg gatgactttg tagcatgggc tgtaacacat gaattatatg gtattgaaaa
      421 tttatcgctt ataccgggta gtgtaggtgc agcagcagtt caaaatattg gagcttatgg
      481 tgttgaagta ggaagtgtta tttttgaagt tcatactatt gatatgttta cattacaaac
      541 acataaattt tctcacaaac aatgtgcgtt tgaatataga aacagcatat ttaagcaagc
      601 acatatgaaa catcatatta tcacagatgt gtgttttgaa ttaaacaaaa agggcagttg
      661 ttctgtttct tatggaagtt tacaacagac tgttttgtct ttaggcgaaa tatcacttca
      721 taatgtacgc aaagcagtaa tagaggtacg taatcaaaaa ttaccaaatc ctaatgaaat
      781 tggtaatgca ggcagttttt ttacaaatcc aattgtaagc aaagatgttt tcaataaaat
      841 tcaacagaag tatcctgata ttgtatggta tgctgcaccc gataatatgg taaaaattgc
      901 tgccggttgg ttgattgaca agcttggatt gaaaggattt agtttgggat tggcacaagt
      961 tcattcaaag caagcattag tacttataaa taaaggaggt gcttcaggtc gtgatattca
     1021 aaaacttgca gaatatattc aagataaagt ctttaatgaa acaggtatag agcttaaacc
     1081 cgaagttatt tatatctaag tttaaatata caaatgtttg gaattattgt tgatataaat
     1141 caatgatttt tattctaata ttttgaataa agatttgcct tttttgaata atttttctaa
     1201 atgttaattt tttttt
//
LOCUS       DAOTOW010000739         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_92915_length_1211_cov_3.460208, whole genome shotgun sequence.
ACCESSION   DAOTOW010000739 DAOTOW010000000
VERSION     DAOTOW010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92915_length_1211_cov_3.460208"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="P5243_10925"
     CDS             complement(<1..453)
                     /locus_tag="P5243_10925"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HRS20009.1"
                     /translation="MDLYVGDVVRYLNDVGGGKVSKILSNTMVEIIDDSGFQIPVLER
                     ELVCVERAQQLESANEMLQVTSEVTTDTVEIEGNDTPHFYLGFVRNTTDSKLFDVYVI
                     NDCNFYANTVVLYRKNNDNHYVQHILIEPNTKVFVQQVSYEEISIISHI"
     gene            514..>1211
                     /locus_tag="P5243_10930"
     CDS             514..>1211
                     /locus_tag="P5243_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRS20010.1"
                     /translation="MIDFTNTSIAEYSYDLPDEKIAKYPLEQRDSSKLLVYKNSTIID
                     SSFSKCIDFLPSGALLIFNNTKVIHARILLQKETGAHIEIFCLEPHTPSNYEQNLSAT
                     ATCTWHCLVGNAKKWKQEPLQTTITIANTLITFTATKKEVSHNSYCIEFSWTGGFSFG
                     DVLEAIGKIPIPPYLHRDTEDIDVLRYQTMYAQHKGSVAAPTAGLHFTPEVISACKQK
                     NISSAFVTLHVGAGT"
BASE COUNT          399 a          240 c          184 g          388 t
ORIGIN      
        1 tatgtgcgaa attatcgaaa tttcttcgta cgatacttgt tgaacaaaga cttttgtatt
       61 gggttctata agtatatgct gcacatagtg attatcatta tttttacgat acaataccac
      121 cgtattggca taaaaattac aatcattaat tacatataca tcaaacaatt tagaatctgt
      181 tgtattacgt acaaaaccta aataaaaatg tggcgtatcg ttaccttcta tttcaactgt
      241 atcggtagtt acttctgatg ttacttgaag catttcatta gccgattcca attgttgagc
      301 ccgttctaca caaaccaatt cacgctctag tacaggtatt tgaaatccgg aatcatcaat
      361 aatttcaacc atggtattgc tcaaaatttt tgaaacttta cccccaccca catcatttaa
      421 atagcgtact acatcaccta cgtacaaatc cataattcaa tttttttttt acttgtcaaa
      481 gttatgtatt tttaccaaaa aatattggtt tcaatgattg attttacaaa tacttctatt
      541 gctgagtatt catacgactt gcccgacgaa aaaattgcca aatatccttt agaacaaaga
      601 gattcgtcga aattgcttgt ttataaaaac agtaccatta tcgacagttc ttttagcaaa
      661 tgtatcgatt ttttaccttc gggagcactc ttaatcttca acaataccaa agtaattcac
      721 gcacgtatac tgttacaaaa agaaaccgga gctcacatag aaatattttg tttagaaccg
      781 catacgccgt ctaattacga acaaaattta tcggcaactg caacatgtac atggcattgt
      841 ttggtaggca atgcaaaaaa gtggaaacaa gaacctctgc aaacaactat tactattgcc
      901 aacactctta ttacatttac cgcaacaaaa aaagaagtaa gccacaattc atattgtata
      961 gaattttcgt ggaccggtgg atttagtttt ggcgacgtat tggaagctat tggtaaaatt
     1021 ccaatccctc cttacttaca cagagatacc gaagatattg atgtattgcg ataccaaacc
     1081 atgtatgctc aacacaaggg atcggttgca gctcctactg ccggtcttca ctttactcct
     1141 gaggttatat ctgcttgcaa acaaaagaat atttcgtcgg catttgtaac actgcatgta
     1201 ggtgcaggca c
//
LOCUS       DAOTOW010000740         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_93037_length_1210_cov_2.977489, whole genome shotgun sequence.
ACCESSION   DAOTOW010000740 DAOTOW010000000
VERSION     DAOTOW010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93037_length_1210_cov_2.977489"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1210)
                     /locus_tag="P5243_10935"
     CDS             complement(<1..>1210)
                     /locus_tag="P5243_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20011.1"
                     /translation="NAVSGVVNIITKKPVTSQGLDVHAHTGTFNTKKIQTGYGYYNAQ
                     QDVGVRVSAQKKYTEKGNFGGSQGLFNWSDSVYNFENDISVDAKITYKSLTFGTNYLQ
                     KQSSVNLYYRTVGTAFKEGESLWNIGFLNSYITYDIKPTAKTIFTHNTYYRNTTLYPN
                     TIAYITDTAQLRCFRPNYLIGTELIGKQQITDNLKCINGIALEHENLAKTYGETWSNS
                     SEETPPRPPKPPLLQNYSANAFVQFSYTIFDQLEFTAGNRFDYNSSYKFVYTPNGSIM
                     FHANRYNIKLLYMEAFRAPKPWDYNWGVGNSHLQPEEIHSLELNSNFSITPHLLFETS
                     VYKNRMYNMLVKNKTNTAWTNADTINLIGIETELQYLRKVYQAYINYTYTYFYDKPDN
                     RIEEIAPHTCN"
BASE COUNT          362 a          170 c          211 g          467 t
ORIGIN      
        1 cattgcatgt gtgaggagca atttcttcaa ttcgattatc gggtttgtcg taaaaatatg
       61 tatatgtata gttaatatat gcttgatata cttttcgtag gtattgtaat tctgtttcta
      121 taccaattaa atttattgta tcagcatttg tccaagctgt gtttgtttta tttttaacaa
      181 gcatattata cattctgttt ttgtacacag atgtttcaaa gagtaagtgt ggtgtaattg
      241 aaaaatttga atttaattcc aacgaatgta tttcttcggg ttgtaaatgt gaattgccta
      301 ctccccaatt gtaatcccat ggtttaggag ctctaaatgc ttccatatac aaaagtttta
      361 tattatatct attagcatgg aacataatag aaccattggg agtataaaca aatttatacg
      421 acgaattata atcaaatctg ttgccggcgg taaattctaa ttggtcaaaa atagtatatg
      481 aaaattgcac aaaagcattg gcagaatagt tttgcagtaa tggtggtttt gggggacggg
      541 gaggtgtttc ttcagatgaa ttactccaag tttctccata ggttttagct aaattttcat
      601 gttctaaagc aattccatta atacatttta ggttgtcagt tatttgttgt ttgccaatta
      661 attctgtacc tattaaataa ttagggcgaa aacatcgcaa ttgagctgta tctgttatgt
      721 atgctatagt atttggatac aatgtagtat ttctataata ggtattgtga gtaaaaatgg
      781 tttttgctgt tggttttatg tcataggtta tatatgaatt taagaatcct atgttccaga
      841 gtgattcacc ttctttaaat gctgtaccta ctgttcgata atagagattt actgaacttt
      901 gtttttgtaa ataattggtg ccaaatgtga gtgatttata agtaatttta gcatcaacgc
      961 ttatgtcgtt ctcaaaatta taaacactgt cagaccaatt gaataatcct tgcgaaccac
     1021 caaaattacc tttttcagta tatttttttt gagccgaaac acgaacacct acatcttgtt
     1081 gagcattgta atatccgtat cctgtttgaa tttttttggt attaaaagtt cctgtatgag
     1141 catgtacatc taagccttgt gaagtaaccg gtttttttgt tattatattt acaactccac
     1201 ttacagcgtt
//
LOCUS       DAOTOW010000741         1208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_93351_length_1208_cov_2.194276, whole genome shotgun sequence.
ACCESSION   DAOTOW010000741 DAOTOW010000000
VERSION     DAOTOW010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93351_length_1208_cov_2.194276"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="P5243_10940"
     CDS             <1..357
                     /locus_tag="P5243_10940"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS20012.1"
                     /translation="GIELPKALNPKGSAKIESGPFVLAMSNLAEAQTSIAEIALQKMY
                     AYPSIVKSMLTIVFDNQSTKNSVQIYTLQGVEVYNSSIVSDSEIRIPATVLTSGMYVV
                     KITSNGKQTLQSIVVE"
     gene            complement(422..>1208)
                     /locus_tag="P5243_10945"
     CDS             complement(422..>1208)
                     /locus_tag="P5243_10945"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRS20013.1"
                     /translation="LFLMTLYCVSSIEKIKSVEVNEEKIGSFMKSINNAFADMSKEEI
                     IQHFVSYEFNRILEYYENTPDINVDSSRESGRERDRDGRNRRDRDKGNTGEERKDRVE
                     RGERRERRFEDVDRSNYTRFFINVGKKEDLDKSKMLKLLNEHANDRDIQYGAIDILKS
                     FSFFEVDSKYTSKILSSFAHATYQGRPISVEIAQAPTGKAARGSKNDFIKGDNYTSKY
                     SRDTDRRDRVGSDRRDRSNSYERGFKSSGRRNKNSDGGGRKRY"
BASE COUNT          358 a          216 c          188 g          446 t
ORIGIN      
        1 ggtatagagt tgcctaaagc tttaaatcct aaaggttctg caaaaattga aagtggacca
       61 tttgttcttg ctatgagtaa tttggccgaa gcgcaaacaa gtattgctga aatagctttg
      121 caaaaaatgt atgcatatcc ttcaattgta aaatcaatgc ttactatagt ttttgataat
      181 caatctacaa aaaatagtgt acaaatttat actttgcaag gcgtagaagt ttataatagt
      241 tctattgttt cagattctga aatacgtatt cctgcaactg tgttaacatc aggaatgtat
      301 gtagtaaaaa ttacaagcaa tggtaaacaa acgttgcaat ctattgttgt agaataagta
      361 taggtatatt aaacaataca tgttacacaa aaaaaagggc tgtttttcag cccttttttt
      421 attagtatcg ttttctgccg ccaccgtcgc tatttttgtt acgtcgtccg cttgatttaa
      481 aacctctttc gtaactgttt gaacggtctc gtctatcaga acctacgcgg tcacgtctgt
      541 ctgtatcgcg ggaatattta cttgtgtagt tgtcgccttt tataaaatca tttttgcttc
      601 cgcgagctgc tttacctgta ggagcttgtg ctatttctac agaaattggt ctgccttgat
      661 acgttgcatg agcaaatgaa cttaaaattt tactcgtata ttttgaatca acttcaaaaa
      721 atgaaaaaga ttttagaata tctatagcac catattgaat gtctctatca ttagcatgtt
      781 cattcaataa ttttagcatt ttgcttttat ctaaatcttc ttttttacct acatttataa
      841 agaatcgagt ataattactt ctatctacat cttcaaatct ccgttctctg cgttctcctc
      901 gttcaacacg gtctttacgt tcttcgccgg tatttccttt atcgcggtcg cgacgattac
      961 gtccatcacg gtcacgttct ctgccacttt cacgtgaaga atcaacattt atatcgggtg
     1021 tattttcata gtattcaagt attctattaa attcgtacga tacaaaatgt tgaataattt
     1081 cttcttttga catatcggca aaagcattgt ttatagattt cataaatgaa cctatttttt
     1141 cttcatttac ttctacagat tttatttttt caattgacga tacacaatac aatgtcatta
     1201 gaaagaga
//
LOCUS       DAOTOW010000742         1205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_93688_length_1205_cov_2.405217, whole genome shotgun sequence.
ACCESSION   DAOTOW010000742 DAOTOW010000000
VERSION     DAOTOW010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93688_length_1205_cov_2.405217"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1205)
                     /locus_tag="P5243_10950"
     CDS             complement(119..>1205)
                     /locus_tag="P5243_10950"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRS20014.1"
                     /translation="SNDWYTHKHHLQPEYNSVILHVVSVYNKPVVNQKHVEIPTLILP
                     ITSRHQYSWNLLSNCCLHKTCNSIFSSIDTFTTNMWFDRLVIERFESKTNTILCDFES
                     TNSSWETTCYRTISRALGNPLNSQPFEMLTQSVSLGILAKHSHSIFQLEAILLGQAGL
                     LHNHSTNTYAIELFKEYTFLQKKYSLTPIAASMWKFSKMRPMSFPPIRIAQLCELIYR
                     SRSLTSHILECTHIQNIITLFDITLHDYWDTHYTLHEQSPYKKKHIGIHTIHSIIINA
                     IIPFLFAYGSKTSQEIYKERALEFLESLPSEQNSILSHWQRLGIQSHNAYRSQALLQL
                     HKEYCNTKRCLHCVFGHTYFSHILKKY"
BASE COUNT          386 a          151 c          224 g          444 t
ORIGIN      
        1 cactttatct ttaaagcttt gaactgttaa atgttctacc attgttttag tttttttaat
       61 ttatactatt gtttatttag cgttttagac gcactaatta aaactcctta catttttttt
      121 aatatttttt taaaatatgt gaaaaatatg tgtgtccgaa tacacaatgt aaacatcgtt
      181 ttgtattaca atattcttta tgcaattgta gtaatgcttg acttctgtat gcattatgag
      241 attgtattcc taaccgttgc caatgcgaaa gtattgaatt ttgctctgac ggaagtgatt
      301 ctaaaaattc aagagcacgt tctttatata tttcttgact ggttttgctg ccgtatgcaa
      361 acagaaaagg gattatagca tttattataa ttgaatgtat ggtgtgtatg ccaatatgct
      421 tttttttata aggagattgt tcgtggagtg tgtagtgtgt atcccaataa tcatgtagtg
      481 taatgtcaaa caatgttatt atgttttgaa tatgtgtaca ctcaagaatg tgcgatgtta
      541 acgaacgaga gcgataaata agttcacata attgagcaat acgaataggt ggaaaactca
      601 taggacgcat ttttgaaaat ttccacatag aagctgctat tggagtaagt gaatattttt
      661 tttgtaaaaa ggtgtattct ttaaataatt ctatagcata tgtatttgtt gaatgattgt
      721 gtaacaagcc tgcttgtcct aatagaatag cctctaattg aaaaatggag tgagagtgtt
      781 tggctaatat tcctaaagag accgattgtg taagcatttc aaaaggttgt gagttaagag
      841 gatttcccaa agctctgcta atagttctat agcaggtagt ttcccacgat gaattagttg
      901 attcaaagtc acagagtatt gtatttgtct ttgattcaaa acgttcaata actaaacggt
      961 caaaccacat attggtagtg aaagtatcaa ttgaagaaaa tatactatta catgttttat
     1021 gtaaacagca atttgataat agattccatg aatattgatg acgtgatgta attggcaaaa
     1081 tgagagttgg aatttctacg tgtttttgat tcacaacagg tttgttatat acagaaacaa
     1141 catgaagtat taccgaatta tattcgggtt gcaaatgatg tttgtgagtg taccaatcgt
     1201 ttgat
//
LOCUS       DAOTOW010000743         1201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_94159_length_1201_cov_3.566318, whole genome shotgun sequence.
ACCESSION   DAOTOW010000743 DAOTOW010000000
VERSION     DAOTOW010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94159_length_1201_cov_3.566318"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..712)
                     /locus_tag="P5243_10955"
     CDS             complement(257..712)
                     /locus_tag="P5243_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20015.1"
                     /translation="MSILCISYACTEYPQYPDIPQVSYLYTIPTITQTDKHIDFTFLL
                     QDGDGDVGLSSADTAKPYTDSLYYNFHSKIRVTKKNNSFILPYSLQYRIPQIRNKNSK
                     KFIKADVTISIDFARNLFPYDTIQLIYYVYDRALHTSNIDTSKAIIFTK"
     gene            complement(729..>1201)
                     /locus_tag="P5243_10960"
     CDS             complement(729..>1201)
                     /locus_tag="P5243_10960"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRS20016.1"
                     /translation="HLAVKNNKGVVVCVNKWDLIEKETNTARDFELKLREKMAPFNDV
                     PIIFTSVHEMLRVQKVLEKTMEVYNNRKQRIPTSRLNEVMLEAVERFHPPSVKGKLIK
                     IKYVTQLPTPFPSFAFFCSNAKYVPESYKRYLENQLRKTYNFEGVPLRIYMREK"
BASE COUNT          380 a          162 c          193 g          466 t
ORIGIN      
        1 tgtagtgctt ttgtcaattg tgttatatgg tattcagaag gcattctttg accattttcg
       61 tattttgaaa gtaaagcttg atcaatcccc gaaagtttac tcacttctaa aattgttaat
      121 tttagttgtt tacgtgtatt ttttagaatg tcttgtaatt tcattatttt tttgaccaat
      181 ttattcatga caaaattagt catttttttt ctgtatttac aaatattttt aatttttagg
      241 ttacgtcgaa ctgacgttat tttgtaaaaa taatagcttt acttgtatct atattgcttg
      301 tatgtaaagc tctatcgtac acatagtaaa tgagttgaat ggtatcgtaa ggaaataaat
      361 ttcgagcaaa atctatagaa atagttacat cggctttaat aaattttttt gaatttttgt
      421 ttcttatttg gggaattcgg tattgtaatg aatatggtaa aataaatgag ttgttttttt
      481 ttgtaacacg aatttttgaa tgaaaattat aatataaact gtctgtatat ggttttgctg
      541 tgtcagcact acttagcccc acatcaccat ctccatcttg caataaaaaa gtaaaatcta
      601 tatgtttatc ggtttgtgtt atagtgggaa tggtatataa ataagatact tgaggaatat
      661 ccggatattg cggatattcg gtgcaagcat acgaaataca aagtatactc aatataagta
      721 taagcaggtt atttttcacg catataaatt cgtaatggaa caccttcaaa attatatgtt
      781 ttacgcaatt gattttctaa atatcgttta taagattcag gaacatattt agcattgctg
      841 caaaaaaatg caaatgaagg aaaaggggta gggagttggg taacatattt aatttttatt
      901 agtttaccct ttaccgaagg aggatggaat cgttcaacag cttccagcat tacttcattt
      961 aatcgagaag ttggaattcg ttgttttctg ttattatata cctccatagt tttttcaaga
     1021 actttttgaa ctcgaagcat ttcatgtaca gaagtaaaaa taattggaac atcattgaaa
     1081 ggagccattt tttctcgcag tttcagttca aaatcgcgag ctgtatttgt ttccttttca
     1141 attaaatccc atttgtttac acacacaaca actcctttat tattttttac agctaaatgc
     1201 a
//
LOCUS       DAOTOW010000744         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_95057_length_1194_cov_2.424056, whole genome shotgun sequence.
ACCESSION   DAOTOW010000744 DAOTOW010000000
VERSION     DAOTOW010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95057_length_1194_cov_2.424056"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="P5243_10965"
     CDS             complement(<1..311)
                     /locus_tag="P5243_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRS20017.1"
                     /translation="MNKYSFSKHVIIANGQFPTHTIPLEIIHNAHTIICCDGAADSLL
                     KHGIEPTIVIGDLDSISTKAKKQFAGKLIHIAEQETNDQTKAVEWAIAQGLQDAVIVG
                     AT"
     gene            complement(315..>1194)
                     /locus_tag="P5243_10970"
     CDS             complement(315..>1194)
                     /locus_tag="P5243_10970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS20018.1"
                     /translation="TYAPDVIHTHLWQSEIVCSQITYSKAIWVTHMHDNMPQLKRIRI
                     PRTKLELTNAYERWIVTMAYKKRNQRFIAISHDTFTYCKRNVPKKLRNAVSLLHNAIN
                     TQLFTHSKKTTTVNNTTLQLVSVGSLVHKKNHAFLIPVVIHLISRGYTVQLNILGEGF
                     LRKELEQEISKQNLTNSIYLQGNTSVYEYYKQADIYVHPATYEPFGLVLIEAMAAGLP
                     VVCLDGKGNRDIIEQGKNGYMLFEQNPILFAEKIIEICQNKEMYSSMSTYAVNFAKQY
                     DIVPYVDQLLQLYTKK"
BASE COUNT          349 a          196 c          212 g          437 t
ORIGIN      
        1 gtagcaccta caattacagc atcttgtaat ccttgtgcta tagcccattc tactgctttg
       61 gtttggtcgt tggtttcttg ttcggctata tgtatgagtt tccctgcaaa ctgttttttt
      121 gctttagttg atatagaatc taaatcacct attacaatag ttggttctat gccatgtttc
      181 aataatgaat cggcagcacc atcgcaacat ataatagtgt gtgcattatg aataatttct
      241 aacggaattg tatgcgtggg gaattgccca ttggcaataa ttacatgttt cgaaaacgaa
      301 tatttattca ttttttattt tttagtgtaa agttgtaaca gttggtctac atagggtaca
      361 atatcatatt gttttgcgaa atttaccgca tacgtactca tagacgaata catttctttg
      421 ttttgacaaa tttctataat tttttcggca aacaaaattg gattttgctc aaacaacata
      481 tacccatttt taccttgttc tataatatct ctattgcctt taccatcgag acacactacc
      541 ggcaagcctg cagccatagc ttctataaga actaatccaa agggctcgta ggtagcaggg
      601 tggacgtaga tatcagcttg tttataatat tcgtatacag atgtattacc ttgtaaatat
      661 attgaatttg tgagattttg tttagaaatt tcttgctcta attctttacg gagaaagcct
      721 tcacctagaa tattcaattg aacagtatac cctcgtgata taaggtgtat aacaacagga
      781 attaaaaatg catgattctt tttatgtact aaactaccaa cactcacaag ttgcagggta
      841 gtattattta ccgttgttgt tttttttgaa tgtgtaaata attgtgtgtt gatagcatta
      901 tgaagtaatg aaacagcatt tcgcagtttt ttgggtacat ttcgtttgca atacgtaaaa
      961 gtatcatgtg aaatagcaat aaaccgttga ttacgttttt tatatgccat agttacaatc
     1021 caacgttcgt atgcgttggt taattcaagt tttgttcgag gaatgcgtat tcgtttaagc
     1081 tgcggcatat tatcgtgcat atgagtaacc catattgctt tagaataggt tatctgacta
     1141 catacaatct ccgattgcca tagatgtgta tgaattacat caggagcata tgtg
//
LOCUS       DAOTOW010000745         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_95174_length_1193_cov_2.970123, whole genome shotgun sequence.
ACCESSION   DAOTOW010000745 DAOTOW010000000
VERSION     DAOTOW010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95174_length_1193_cov_2.970123"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="P5243_10975"
     CDS             <1..914
                     /locus_tag="P5243_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS20019.1"
                     /translation="VTGVSGSGKSSLINHTLHPILQKHFYNSKQEPLPYSSIQGLEHI
                     NKVIEVNQSPLGRTPRSNPVTYTNIFNDIRKLFEQLPDSKIRGYKAGRFSFNIKGGRC
                     ETCKGAGVKTIEMNFLPDVYIECDDCGGKRYNRETLEVRFKGKSISDILNLTINLAVD
                     FFENVPHIYTKLKTLQDVGLGYLTLGQASTTLSGGEAQRVKLAAELAKKDTGNTIYIL
                     DEPTTGLHFQDIDILLGVLQKLVNRGNTVIVIEHNLDVIKVADYIIDVGPEGGKNGGF
                     IVGSGTPEEITTISTSYTGEFLKKELF"
     gene            complement(950..>1193)
                     /locus_tag="P5243_10980"
     CDS             complement(950..>1193)
                     /locus_tag="P5243_10980"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HRS20020.1"
                     /translation="MMGITTAVMGGIIRDVLCNDIPLIFHKEIYATACLLGGIIYLLL
                     LYCNVSESIIIITAATSIFIVRTLSVRYKWQLPKFK"
BASE COUNT          472 a          182 c          192 g          347 t
ORIGIN      
        1 gtgttacagg agtatcgggc agtggaaaat caagtcttat aaatcataca ttacatccaa
       61 ttttacaaaa acatttttac aattctaaac aagaacctct cccctatagt tctatacaag
      121 gattagaaca tataaacaaa gttattgaag taaaccaatc gcctcttggc aggactccac
      181 gttcaaatcc ggttacatat acaaatattt ttaatgatat tcgtaaatta tttgaacaac
      241 tacccgattc aaaaataaga ggatacaaag ccggacgctt ttcatttaat ataaaaggtg
      301 gccgatgcga aacatgcaaa ggagcaggtg taaaaactat tgaaatgaat tttttacctg
      361 atgtatatat agaatgtgat gattgtggcg gcaaacgata taatagagaa acgttagaag
      421 tacgatttaa aggtaaatca ataagtgata ttttaaactt aacaattaat cttgctgtag
      481 actttttcga aaatgttcct catatataca caaaactcaa aacattgcaa gatgttggtt
      541 taggctattt aacattagga caagcttcta caacactttc aggtggtgaa gctcagcgag
      601 taaaattagc agccgaatta gcaaaaaaag atacaggtaa tactatatat attcttgatg
      661 aaccaactac agggttacat tttcaagata tagacatact attgggggta ttacaaaaat
      721 tagtgaatag aggaaataca gttatagtaa ttgaacataa cttagatgta ataaaagtag
      781 cagattatat aatagacgta ggtcccgaag gtggaaaaaa cggaggattc attgtaggtt
      841 cgggaacacc tgaagaaata acaactatat caactagtta cacaggcgaa tttcttaaaa
      901 aagaattatt ctgatgctca ggtaatttag tgaaaacaaa acactaaatt tatttaaatt
      961 ttggtaattg ccatttgtaa cgaacactta aagttcgcac aataaatatt gatgttgctg
     1021 cagtaattat aataatagat tcagaaacat tacaatacaa caacaaaaga tatataatac
     1081 cacctaaaag acatgccgta gcataaattt ctttatgaaa aataagagga atatcattac
     1141 ataatacatc acgaattatt cctcccatta cagcagttgt tatacccatc att
//
LOCUS       DAOTOW010000746         1189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_95661_length_1189_cov_3.265432, whole genome shotgun sequence.
ACCESSION   DAOTOW010000746 DAOTOW010000000
VERSION     DAOTOW010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95661_length_1189_cov_3.265432"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="P5243_10985"
     CDS             complement(<1..1163)
                     /locus_tag="P5243_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20021.1"
                     /translation="MKFFKFILISCVWILLPYASSAQYLHFAGGYMFSVFVCNNQQLY
                     TWGDNYYEQLARNNNTCAYRMPCEAPIPYSLTSIDAGFGGFCAGVTNNNQVVTWGSNY
                     YGELGSGQNCTPLCTRIQAERVLGGQTGNTYLEMVKTVSIGQSHAYALLNTGEVVAWG
                     SNAYGQLGDGTTINKNSPVFVKISSSERLQNIVMISAGANHGYALTANGYVYAWGNNQ
                     NNQLGCGNSNSQTYAKLVVDKNNNPVTGITAIDGGRNFGLLLRNSSMVMGLGAYKGSD
                     YGPKGKIYTTYPYAELITGGQTPNYYLENVIAISAGYNHSMAIVKEQNQTYVVAWGDN
                     RFDELSDDTGGQIGTGNFTAEQYFTPQYVLRLANTKLTGATAIVAGCGVSYIQT"
BASE COUNT          348 a          243 c          200 g          398 t
ORIGIN      
        1 gtttgaatgt atgaaacacc gcatcctgct actatagccg ttgctccggt aagttttgta
       61 ttagccaaac gtaaaacata ttgtggggta aagtattgtt cggctgtaaa atttcctgta
      121 cctatttgac ctcctgtatc atcagaaagt tcatcaaatc tattatcacc ccaagctact
      181 acataggtct gattttgttc ttttacaatt gccatgctat gattgtatcc ggccgaaata
      241 gctattacat tttctaaata ataatttgga gtttgtcctc ctgttatcag ttcagcatat
      301 gggtaggttg tgtaaatttt accttttgga ccatagtcac tgcctttgta tgcacctaat
      361 cccataacca tagagctatt tcttagcagt aagccaaaat tcctgccacc atcaatagct
      421 gtaattcctg taacgggatt attgtttttg tcaacaacta acttcgcgta ggtttgtgaa
      481 ttagaattgc cacaacctaa ttggttattt tgattgtttc cccaagcata tacatagccg
      541 tttgcagtaa gagcatagcc gtgattggct cctgcagaaa tcattacaat attttgtaat
      601 cgttcagagc tggaaatttt cacaaaaaca ggtgaatttt tatttattgt tgtaccatct
      661 cccaattgac catatgcatt gcttccccaa gctactactt cgcctgtatt tagtaaagca
      721 taggcatgcg attgaccaat actaactgtt tttaccattt ctaagtaggt gttgcctgtt
      781 tgtcctccta aaaccctttc tgcttgtatg cgtgtacaca aaggagtaca attttgtcca
      841 ctgcctaatt cgccataata atttgacccc catgttacaa cttgattgtt gttggttact
      901 cctgcacaaa aacctccaaa gccggcatct atactcgtaa gtgaataagg aataggagct
      961 tcacatggca tacggtatgc acatgtatta ttatttcttg ctaattgttc atagtagttg
     1021 tcaccccaag tatataactg ttggttattg catacaaata ccgaaaacat atatccgcca
     1081 gcaaaatgca aatactgagc agatgatgca tatggaagta atatccatac acaagaaata
     1141 agtatgaatt taaaaaattt catacggtat agtatacgat attccccat
//
LOCUS       DAOTOW010000747         1187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_95886_length_1187_cov_3.673145, whole genome shotgun sequence.
ACCESSION   DAOTOW010000747 DAOTOW010000000
VERSION     DAOTOW010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95886_length_1187_cov_3.673145"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..>1187
                     /locus_tag="P5243_10990"
     CDS             150..>1187
                     /locus_tag="P5243_10990"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRS20022.1"
                     /translation="MKYSFTLIFLILSISVWAQQTCSIKIFVKQQNQQVLSGAQIYAQ
                     KSHVSFITNEKGFALCVIPCKPDTLLISYIGYKDVYVALPMISDTIIHVIMSIEEIGI
                     NKVVVTQVKENSQITNTLGELQVKHSELRSIPGIMGEPDVLRTLSLSSGVKQIEGMQG
                     IFVRGGSQDQNLIVYDGATVYNPSHLLGFFSVFNSDIVASASLFSSGAPALYGGRLSS
                     VLTIQSLSAIPDSVETAVNLGVLSSRVMLNVPISQKSAIRASFRKTYLNLFVMPIVEK
                     SLELKDEVSTQFGFYDATCRFDFKPNLLHQFYISAYLGKDKFGLLNSQTLLTNNVNWG
                     NFIVAAQWKYNA"
BASE COUNT          382 a          152 c          201 g          452 t
ORIGIN      
        1 aaataaattt tgttttatta aattaatttt gtagttttat cggacaataa tgtttttttt
       61 tagtcttttt tttaagtttt caaatagcat gattttttga ttattttttg taaaaaacat
      121 ataattttac agatattaaa ttttgcaatt tgaagtatag ttttacactt atctttttaa
      181 ttttaagtat atctgtttgg gcacaacaaa catgttcgat aaaaatattt gttaaacaac
      241 aaaatcaaca agttctttcc ggtgctcaaa tatatgctca aaaatcacat gtaagtttta
      301 ttaccaatga aaagggattt gcgttgtgtg taattccttg taaacctgat acacttttaa
      361 tttcatatat tggttataag gatgtgtatg ttgcattacc tatgatttcc gatacaatta
      421 tacacgttat tatgagtata gaagaaatag gtataaacaa agtagttgtt actcaagtaa
      481 aagaaaattc tcaaataaca aatacattgg gagaattaca agtaaagcat tctgaattgc
      541 gaagtattcc cggtattatg ggagaaccgg atgtgttacg tactctttct ttaagtagtg
      601 gagtaaaaca aatagaagga atgcagggta tttttgtaag aggtggaagt caagatcaaa
      661 atcttattgt atatgatggt gctacagtgt ataatccttc tcatttgtta ggcttttttt
      721 ctgtatttaa ttcagatata gttgcatcgg cttcattatt ttcatcgggg gcacctgcat
      781 tatacggagg tagattatcg tcggtgttaa ctatacaatc attatccgca attcctgata
      841 gtgttgaaac tgctgttaat cttggtgttt tgtcttctcg tgttatgctc aatgttccaa
      901 tttcacaaaa aagtgcaatt cgagcttctt ttcgtaaaac gtatctgaat ttgtttgtaa
      961 tgcctatagt tgaaaaatct cttgaactca aagatgaggt gtcaacacaa tttggatttt
     1021 atgatgcaac gtgtcggttt gattttaaac caaatttatt acatcaattt tatatttcgg
     1081 catatctggg aaaagataaa tttggattat taaattctca aacattgctc acaaataatg
     1141 taaattgggg taattttatt gttgcggctc aatggaaata caatgcg
//
LOCUS       DAOTOW010000748         1183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_96389_length_1183_cov_3.305851, whole genome shotgun sequence.
ACCESSION   DAOTOW010000748 DAOTOW010000000
VERSION     DAOTOW010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96389_length_1183_cov_3.305851"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1183)
                     /locus_tag="P5243_10995"
     CDS             complement(<1..>1183)
                     /locus_tag="P5243_10995"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20023.1"
                     /translation="ASGVFSPNFKGETSGNYTIEYTYTDTKGCVGKATKVIPVQFTPA
                     PGKKDYFALKDPSATVIIEATNLETNFEKVQWYDASSTAKSTTNPFATGDNANTEVSK
                     KYYVTQFVNGCESKKTEVAVDIIDCPIPAPNVTVTPSATICNYDNTPTIQASVGSPWL
                     APGGRPSGAVEFRYYTQATGGTPIGTSASGSYTPTIDKSIAKVHQYFVSEYNPNITPK
                     PCESPRTAVTITVNKTLPIQIIGQKDVCERPNEQNPTLQAKDEIGNATVEWYENQPTV
                     PATTTPNSIGKNYTPSFTSVGTHTVYAAQRVNGCMSDLAQASFTIKAIPPAPTTVNNE
                     ICEGFPHVTISATGTNIIWYQNADKTGQLATGTQYKPTVTTTKTFFATQTVQGCESTT
                     AP"
BASE COUNT          298 a          202 c          237 g          446 t
ORIGIN      
        1 caggtgcagt tgtactttca catccttgaa ctgtttgtgt agcaaagaat gttttagtag
       61 ttgtaacagt aggtttatac tgtgtacctg tagctaattg acctgtttta tccgcatttt
      121 gataccaaat aatattagta cctgttgccg atattgttac atgaggaaac ccttcacaaa
      181 tttcgttgtt tactgttgta ggtgccggtg gaatagcttt aattgtaaat gaagcttgag
      241 ctaaatcact catacaaccg tttactcgct gtgctgcata tacagtatgt gttcctacgc
      301 ttgtaaaact tggtgtataa tttttaccta tactgtttgg ggttgtagta gcgggaactg
      361 ttggttgatt ttcataccat tcaactgttg cgttacctat ttcgtcttta gcttgtaatg
      421 taggattttg ttcgtttgga cgctcacata catctttttg acctatgatt tgaataggta
      481 gagttttgtt tacagtaata gtaacagcag tacgaggact ttcacatggc ttaggtgtta
      541 tgttaggatt gtactcgcta acaaagtatt gatgtacttt ggcaatactt ttatcaatag
      601 taggagtata tgaacctgat gcacttgtgc caataggtgt tcctccggtt gcttgtgtat
      661 agtatctaaa ttctaccgca ccggaaggtc tgccacccgg tgctaaccat ggtgagccta
      721 ccgaagcttg aattgttgga gtattatcat aattacaaat tgtagcactt ggtgttacgg
      781 taacgttagg tgcaggtatt ggacagtcta taatatcaac cgctacttct gtttttttac
      841 tttcgcaacc gtttacaaat tgagtaacat aatacttttt gcttacttct gtgtttgcat
      901 tgtctcctgt ggcaaatgga tttgtagtag attttgctgt gctagaagca tcataccatt
      961 gtactttttc gaaatttgtt tccaaatttg tagcttctat tattactgtt gcacttgggt
     1021 cttttaatgc aaaataatct tttttgccgg gagcaggtgt aaattgtaca gggattactt
     1081 ttgttgcttt tccaacacat cctttagtat cggtatatgt atattcaata gtataatttc
     1141 cacttgtttc tcctttaaaa tttggtgaaa atacacccga agc
//
LOCUS       DAOTOW010000749         1179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_96922_length_1179_cov_3.357651, whole genome shotgun sequence.
ACCESSION   DAOTOW010000749 DAOTOW010000000
VERSION     DAOTOW010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96922_length_1179_cov_3.357651"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..>1179)
                     /locus_tag="P5243_11000"
     CDS             complement(246..>1179)
                     /locus_tag="P5243_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS20024.1"
                     /translation="NSFLVITICTNAFSQNCDSLYVRSLKQFDASNIKESAQTIQTCI
                     QQCAAHTKYYMHAAKCWYQLKDYEKTIQYLQSAIAIDSTHIPAYALRAQIYMNAELYS
                     KAIPDYEKIVSLITHLDSTTAVYRINLSKAYLNTSAYDKAYTLLKELYEYDAKNLELL
                     TNLSVCAMKLDKESDAALYLQQILAINPQYTPGLINYGFFMIEKQEFQKAIEYLNKAL
                     TIQPREAYALNNRGYAYYKLGKYDNALEDITTSISYDPSNSYAYRNRAYVYSTTGLTK
                     EACADIQKAIQLGYIAMYGNDILQLQKELCISKK"
BASE COUNT          355 a          187 c          177 g          460 t
ORIGIN      
        1 cctagtccta acaagagtac ggcacttaat tttaatcgct tgtgtcgcat ttttgtcctc
       61 cttttttaat tgtttttact cattttattt atctgttatg cttactgttt cttgtttgct
      121 ctgcgttttc tccactgaat gctaactaat atgtatatca aactcctttt caattgcttt
      181 atgtaacaac tgtgttgtag gatttgaatc ccatgtttga gcattggtat caaaacgatt
      241 attcattatt tttttgaaat gcacaattct ttttgtaatt gtaatatatc attaccatac
      301 atagctatat atcctaactg tatagctttt tgaatatcgg cacatgcttc tttagtcaaa
      361 cctgttgtgc tgtatacata ggctctattt ctatacgcat atgaatttga aggatcgtaa
      421 gaaatagaag tagtaatatc ttctaaagca ttgtcatact tacctaactt ataatatgca
      481 taccctctgt tgtttaatgc ataagcttca cgaggttgta ttgtcaaggc tttattcaaa
      541 tattctatag ccttttgaaa ttcttgtttt tcaatcataa aaaaaccgta gtttattaaa
      601 ccaggtgtgt actgaggatt aatagcaaga atttgttgta aatataatgc cgcatcagat
      661 tctttatcta atttcatagc acataccgac agatttgtga gtaattccaa attttttgcg
      721 tcgtattcat ataattcttt taaaagtgta taagctttat catatgcact tgtattaaga
      781 tacgctttac ttagattaat tctatataca gctgttgttg aatcaagatg agtaatgagt
      841 gatactattt tttcataatc aggaatagct tttgaataca attctgcatt catataaatt
      901 tgagctctca gagcatatgc tggtatatgg gtactatcta ttgcaatagc cgattgcaaa
      961 tattgaatgg ttttttcata atctttcagc tggtaccaac atttagccgc atgcatataa
     1021 tattttgtat gagcggcaca ttgttgtata cacgtttgaa ttgtttgtgc cgattcttta
     1081 atattagatg catcaaattg ctttaaagaa cgcacataca acgaatcaca attttgagaa
     1141 aatgcatttg tacaaatagt tataaccaaa aaagaattg
//
LOCUS       DAOTOW010000750         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_97322_length_1176_cov_3.058876, whole genome shotgun sequence.
ACCESSION   DAOTOW010000750 DAOTOW010000000
VERSION     DAOTOW010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97322_length_1176_cov_3.058876"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="P5243_11005"
     CDS             <1..279
                     /locus_tag="P5243_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20025.1"
                     /translation="YVKSDLRHSHLFPILQRRRQMMCDIILMAHGFKTTPPQFFTFIT
                     DYVIFKVNDSPDIRKMDLGTNYDIVSKTVEQVRAMAEKQYNVFKWIKV"
     gene            276..476
                     /locus_tag="P5243_11010"
     CDS             276..476
                     /locus_tag="P5243_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20026.1"
                     /translation="MTCKKSITKQELAQVLNISHGTLRRFLNIDLYAALEPLGYKKNT
                     NILTGKVLQYIKEFYCLHDDEF"
     gene            514..726
                     /locus_tag="P5243_11015"
     CDS             514..726
                     /locus_tag="P5243_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20027.1"
                     /translation="MTTLVLTTNNKTDLKLFLDLAKRVGVQSKTLTDEEILDAGLLKA
                     MKEAKTTKIVSKSQIMKSLTRNVSKV"
     gene            710..976
                     /locus_tag="P5243_11020"
     CDS             710..976
                     /locus_tag="P5243_11020"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRS20028.1"
                     /translation="MLVKFRERFDKDIHDITDKDVLDSIIEVIENVELAKKPQDITNI
                     KKIKGHKQAFRIRIGNYRIGVFIEKGVVEFARILQRKDIYKKFP"
BASE COUNT          471 a          168 c          189 g          348 t
ORIGIN      
        1 tatgtaaaat cggatttacg gcattctcat ttgtttccaa ttttgcaacg gcgtaggcaa
       61 atgatgtgtg atattatact tatggcacac ggcttcaaaa ccacaccgcc acagtttttc
      121 acatttatta cagattatgt aatattcaaa gtaaacgatt cgcccgatat acgaaaaatg
      181 gatttaggaa ccaactatga tatagtttct aaaactgtag aacaggttcg tgcaatggca
      241 gaaaaacaat ataatgtatt caaatggata aaagtatgac atgtaaaaaa tcaattacca
      301 aacaggagct tgcacaagtt ctcaatatat cgcatggtac attgcgtaga tttttgaaca
      361 tagatttata tgcagcactc gaaccgcttg gatacaaaaa aaatacgaat attctaacag
      421 gtaaggtatt gcaatacata aaagaatttt actgcttgca tgatgatgaa ttttagttgt
      481 atatttgcat tgtaaacaag aaaggagcat agtatgacaa cattagtttt aacaacaaat
      541 aataaaacag acttaaagct atttttggat ttagcaaaaa gagtaggcgt acaatcaaaa
      601 acattaacag acgaagaaat acttgatgct ggattgttaa aagccatgaa agaagctaaa
      661 acaactaaaa ttgttagtaa aagtcaaatt atgaaaagtc ttacacgcaa tgttagtaaa
      721 gtttagagag cgattcgata aagatattca tgatattacc gataaagatg tactcgatag
      781 tataattgaa gtaatagaaa atgttgaatt agctaaaaaa ccacaagaca ttactaatat
      841 taaaaaaata aaaggtcata aacaagcctt tcgtattcgt attggtaatt accgcatagg
      901 tgtttttata gaaaaaggag ttgttgaatt tgctcgcata ctacaacgaa aggatattta
      961 taaaaaattt ccataaactt tttttaccca accccttgac tttggcattg taattttatt
     1021 tgcttttacc acacaaaaaa acatatattt acactataac aagaaaggag catagtatga
     1081 caacaattgc aataaatgaa aagactgtaa aaggtaaaag cctgttaaaa ttccttgagc
     1141 aatttaaagg cgaaaacttt attacaatta ttgata
//
LOCUS       DAOTOW010000751         1164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_98894_length_1164_cov_2.924256, whole genome shotgun sequence.
ACCESSION   DAOTOW010000751 DAOTOW010000000
VERSION     DAOTOW010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98894_length_1164_cov_2.924256"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="P5243_11025"
     CDS             complement(<1..704)
                     /locus_tag="P5243_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRS20029.1"
                     /translation="MKIKLVCTVLFLFVTILLIQAQSSTQYIRMNQLGFLPYAQKQAA
                     IVNTNASSFNILNQANEIVFEGLLSSTGFWSASGESVKIADFTILTNPGTYTLQVPGY
                     DSSSPFVIHDTVYNQVNKAIVKAFYFNRASTALLEEHAGIYKRKAGHPDTAVVIHPSA
                     VGPVRIAGQKISTPKGWYDAGDYNKYIVNSGITVGTLLQAYENYKQYWDTITWNIPES
                     NNSIPDLLDEIKWNLD"
     gene            complement(920..991)
                     /locus_tag="P5243_11030"
     tRNA            complement(920..991)
                     /locus_tag="P5243_11030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(956..958),aa:Arg,seq:cct)
BASE COUNT          376 a          179 c          176 g          433 t
ORIGIN      
        1 caatctaaat tccattttat ttcgtcaagt aaatcgggaa tggaattgtt actttcgggt
       61 atattccatg ttatggtatc ccagtactgt ttataatttt cgtatgcttg taatagtgtg
      121 ccaacagtaa ttcctgaatt tacaatatat ttattgtaat ctccggcatc gtaccaacct
      181 ttaggtgtac ttattttttg accagcaatt cgtaccggtc ctacagccga tgggtgtatt
      241 acaacagctg tgtcagggtg accggctttt cgtttgtata tacctgcatg ttcttcaagt
      301 aaggctgttg aagccctatt aaaataaaat gcttttacta ttgccttatt tacttgatta
      361 tatactgtat cgtgtattac aaatggtgat gacgaatcat aaccaggtac ttgcaatgta
      421 tatgtgcccg gatttgtaag aatagtaaaa tcagctattt ttactgattc acccgaggca
      481 gaccaaaatc cggtagagga aaggagacct tcaaaaacaa tttcatttgc ttgattgaga
      541 atgttgaatg aacttgcatt agtgtttact attgctgctt gtttttgtgc atacggtaaa
      601 aaacctaact gattcattcg aatatactgt gtgcttgatt gagcttgaat taacaatatt
      661 gttacaaaca aaaacaatac tgtacatact aattttattt tcatatctat tctccaaatt
      721 ttcaataaaa atactcacta tttttattaa acattactta atactattta aaagcgtttt
      781 ttaatttggt aaatgatata atttattggg taagatatca aaatggcacc tttattttgt
      841 tatataatta ataatataca gaatagatgg aaaaagtatg attgtgtata ataagaatac
      901 aaatgtgtgt tttaatttgt agcctcgccg ggaatcgaac ccgaatttaa agtttaggaa
      961 actttcgttc tatccattga actacaaggc ctcaattgtt tttttgcaaa aatacaacat
     1021 tgttcaatat tcatatcgtg tttttttata cttttgaata acaattccaa attaaaatac
     1081 atatgaaaaa attttttatt ctcattctct taatcatagg acttttttat aacggtattt
     1141 cacaaaatca gcctaaaaat atta
//
LOCUS       DAOTOW010000752         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_99806_length_1157_cov_3.361162, whole genome shotgun sequence.
ACCESSION   DAOTOW010000752 DAOTOW010000000
VERSION     DAOTOW010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99806_length_1157_cov_3.361162"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="P5243_11035"
     CDS             complement(<1..975)
                     /locus_tag="P5243_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20030.1"
                     /translation="MKLKDILNLFKTEEEIRMQRDSIIREFSPYYDLDSTVAKYVIDS
                     LLRQCKHLFSMLDIPESVNLLALQKELKTVYSIGIIDIQKPYKTLVVDKKPQKIRMHI
                     EQVLTIGEAHAKVISAIPQSLDSITRNKVKDIIYKSIKPNIIYNDAISNERKSLLLSK
                     ISEKHGYVSEGSRIISKGTVVTEKDIKVLDSLKKEYENSSMTIHTMIAIFTGQIILIF
                     MAFIVLVLLILYFEPHILQNHAQTLFIVSIVVLFVAMASITQKFNHISIYIIPYVALP
                     LVVKNFLNARIALFVHVITMLIIGFIAPNSFDFVFLQIIVGSIALFSIG"
BASE COUNT          424 a          168 c          148 g          417 t
ORIGIN      
        1 tcctatacta aataatgcaa tagacccaac tataatttgc aaaaaaacaa aatcaaaact
       61 attgggggct ataaaaccta taataagcat agtaataaca tgaacaaaca gagcaattcg
      121 agcattcaaa aagtttttta caacaagagg caaagcaaca tacggtatta tataaatact
      181 aatatgatta aacttttgag taatacttgc catagcaaca aacaaaacca ctatactcac
      241 tataaataat gtttgagcat gattttgaag aatgtgaggc tcaaaatata atataagcaa
      301 aaccaataca ataaaagcca tgaagataag aataatttgc ccggtaaaaa tggcaatcat
      361 agtatgaata gtcattgatg aattttcata ctctttttta agtgaatcaa gaacttttat
      421 atctttttcg gtaacaacgg tacccttgct tattatacga gaaccttcac ttacatatcc
      481 atgtttttca gatattttag aaagcaataa agattttcgt tcattagaaa tagcatcatt
      541 ataaatgata tttggtttaa ttgatttata gattatatct tttactttgt tacgagtaat
      601 agaatcaagt gattgaggaa ttgcagaaat tacttttgca tgagcttcgc caatagttaa
      661 cacttgttct atatgcattc gtattttttg aggtttttta tctacaacga gtgttttata
      721 tggcttttga atatcaataa tcccaataga ataaactgtt ttaagttctt tttgcagtgc
      781 taacaaattt accgattcgg gtatatcaag cattgaaaac aaatgcttgc attgccttaa
      841 taatgaatca attacatact tagcaacagt agaatccaaa tcataatatg gagaaaattc
      901 acgaattata ctatcacgct gcattcttat ttcttcttcg gttttaaata aatttaatat
      961 atcttttagc ttcatgtttt ttttattatg ttttcaaatt tactataaag atacaattac
     1021 gcatcatttt atttactaaa ttatttagta atagaggatt tgtaaatggc tattttcact
     1081 atttttgaaa aatgaaaatc attcaaatct tttttctatt actttcgttt tcagtctttt
     1141 cacaatcaac gtttgaa
//
LOCUS       DAOTOW010000753         1156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_99937_length_1156_cov_2.985468, whole genome shotgun sequence.
ACCESSION   DAOTOW010000753 DAOTOW010000000
VERSION     DAOTOW010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99937_length_1156_cov_2.985468"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1156
                     /locus_tag="P5243_11040"
     CDS             <1..>1156
                     /locus_tag="P5243_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20031.1"
                     /translation="FYVSYVATETNTGKQCESPKSKVSVVVNPLPDFTIQLSKTGTIC
                     YDDSDVEISVIPNTGTTISSVNYDINTDPNRVQKSGIKGYINPDAPVMGYTIPGLYPI
                     QINAEVTNQFGCKITKNITNSVRWTSIPTITNVTEVLDIANTNPITITATGMAVNWYN
                     SASKTGSLATNTNSYTTSITRTTVSNTTLYATQTLDGCESQTALGTIQVIDCPFAAPD
                     ATSIAACPGLPLADITATTTNTAIQWKWFDESNTVLPTSTATLTHGVANTTETIKKFY
                     VQYEAYDSGSGKTCWSPKKEVTVQVYKKPMVFFTDAVHTLCYDSGPHFLENIVNMGNG
                     NPNITWTINGETTGIKNGVVDPTFNGAQSGNYTVEAIITDSKGCTSTETC"
BASE COUNT          420 a          205 c          205 g          326 t
ORIGIN      
        1 atttctatgt aagctatgta gctaccgaaa caaatacagg caaacaatgc gaaagtccta
       61 aatcaaaagt atcggtggtg gtaaatccgt tgcctgactt tacaatacaa ctcagtaaaa
      121 caggtactat ttgttatgac gattctgatg tagaaatatc agtgattcca aataccggaa
      181 caactattag ttctgtaaat tatgacataa ataccgatcc aaatagagta caaaaatcgg
      241 gaattaaagg atatatcaat cctgatgctc ctgttatggg atacactatt cctggattgt
      301 atccaattca aattaatgct gaagtaacca atcaatttgg atgtaaaatc actaaaaata
      361 ttactaattc tgttcgatgg acaagtattc cgactattac aaatgttact gaagtacttg
      421 atattgccaa tacaaatcca attacaatta cagctaccgg aatggctgta aattggtata
      481 attctgcaag taaaacaggt agtttggcaa ccaatacaaa ttcgtatacg acttctatta
      541 cacgtactac agtaagcaat accactctct atgctacaca aacattagat gggtgcgaaa
      601 gccaaactgc attgggtaca attcaagtta ttgattgtcc gtttgctgct cccgatgcta
      661 catctattgc tgcatgcccg ggattaccat tggctgatat tacagcaaca actacaaaca
      721 cagctattca atggaaatgg tttgatgaat ctaataccgt tttaccaact tcaacagcca
      781 cgttaactca tggagtagca aacacaaccg aaactattaa aaaattctat gtgcaatatg
      841 aagcatatga tagtggaagc ggaaaaacct gttggagtcc taaaaaggaa gtaactgttc
      901 aggtatataa aaaaccaatg gtatttttta cagatgctgt tcatacactt tgctatgaca
      961 gtggacctca ttttttagaa aatatagtaa atatgggtaa tggtaatcca aatattactt
     1021 ggacaataaa tggagaaact acaggtataa aaaatggagt agttgacccg acatttaatg
     1081 gagctcaaag cgggaactac acggtagaag ctataattac cgatagtaaa ggttgtacaa
     1141 gtaccgaaac atgtat
//
LOCUS       DAOTOW010000754         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_100597_length_1151_cov_2.785584, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000754 DAOTOW010000000
VERSION     DAOTOW010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100597_length_1151_cov_2.785584"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1151)
                     /gene="zwf"
                     /locus_tag="P5243_11045"
     CDS             complement(<1..>1151)
                     /gene="zwf"
                     /locus_tag="P5243_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRS20032.1"
                     /translation="HSTPPDKSKDFLHLCYYIQSEFQSYTSLELFVEQLYEIDIKIQT
                     KGNFIFYLSTPPSLYEPIVTGLGKAKLNISSERFPGWKRLIVEKPFGKDVASAHKLQQ
                     HLISHFDEEQIYRIDHYLGKETAQNILVTRFYNTIFEPLWNRNYIHHVEITSAEQEGI
                     GNRGGYYDGSGALRDMLQNHLLQLVALTAMEPPTNFNADSIRNEVLKVFQSLRPLNLD
                     NLKRQVVRGQYTQSHIDGILKKAYTEELDIPANSRTETYVALKFFIDNWRWGGVPFFI
                     RTGKNLPTRVTEIVITFKSIPHPVFVKTASTQESKNQLIIRIQPDEGIAFRFGVKIPG
                     IGFDVETTNMNFKYAQKEGIELPSAYERLLHDCMQGDASLFTRGDAVDLA"
BASE COUNT          348 a          191 c          202 g          410 t
ORIGIN      
        1 gctaaatcta ctgcatcacc acgtgtaaag agtgaagcat ctccttgcat gcaatcatgt
       61 aacaaccgtt catatgccga aggtagctca attccttctt tttgagcata tttaaagttc
      121 atgtttgtag tttcaacatc aaaacctatt ccgggtattt taactccaaa tctaaaagca
      181 ataccttcgt cgggttgaat tctaataata agttgatttt ttgattcttg cgtacttgca
      241 gtttttacaa acacaggatg aggaatactt ttaaatgtaa taacaatttc tgtaactcga
      301 gttggtaaat ttttgcctgt tcttataaaa aatggtacac cgccccaacg ccagttgtca
      361 ataaaaaatt taagagcaac gtaggtttct gttcttgaat tagcaggaat atcaagttct
      421 tctgtgtatg ctttttttaa tattccgtca atatgagatt gtgtatattg tccacgaact
      481 acttgtcgct ttaaattatc gagattgagt gggcgtaaag actgaaaaac tttgagtact
      541 tcgttacgaa tagaatctgc attaaagttt gttggtggtt ccatggctgt aagagcaact
      601 aattgcaata aatgattttg cagcatatct cttaatgctc ccgaaccatc atagtagcca
      661 cctctgtttc caataccttc ttgttctgcc gatgttattt ctacatgatg aatgtagttc
      721 ctattccaca aaggttcaaa tatagtgtta taaaagcggg taacaagaat attttgtgct
      781 gtttcttttc ctaaataatg gtcaatacga taaatttgct cttcgtcaaa atgtgaaatt
      841 aaatgttgtt gcaatttgtg agccgaggca acatctttcc cgaatggttt ttctactatt
      901 aatcgtttcc atccgggaaa acgttcagat gaaatgttga gttttgcttt gcctaatcct
      961 gtaactattg gttcatagag cgatggtgga gttgataaat aaaatataaa atttcctttt
     1021 gtctgaattt ttatatcaat ttcatataat tgttctacaa acaattcaag tgatgtataa
     1081 ctttggaatt cgctttggat ataatagcat agatgcaaaa aatcttttga tttatcagga
     1141 ggagtagaat g
//
LOCUS       DAOTOW010000755         1147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_101270_length_1147_cov_1.641941, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000755 DAOTOW010000000
VERSION     DAOTOW010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101270_length_1147_cov_1.641941"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="P5243_11050"
     CDS             complement(<1..1096)
                     /locus_tag="P5243_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20033.1"
                     /translation="MELIDLLEQHSNTVYNKHVNSKIHIHFSQDIIDITNKKPHAILL
                     GYKENRNSTIASTLQIADEFRVSLEKLCATSESLTIVDIGNCKQGKTVRESYIILQEV
                     LKILKELQIPIIIIGGTQESLYYIGKEILKKVQYPTICCIDSKIDLDIDSEDFSNTNY
                     ISKLVNLSETQRLISIAYQEYLSIQKTYEWFKSQYFPLVRLGKVRQNILEVEPLIRDS
                     QIVSFDMSAIRYSDMPSQIYSNPNGLYAEESCQLAWYAGYSPNIRVFCLHDTFFNEST
                     KVLTATLTAQIIWHFLDGISQQKQKKCDFSAEKFTKYYVQNNFLEQDITFFENIYTKQ
                     MWVEIPLKNKKKKRILPCSSLDYQVFLDNNL"
BASE COUNT          379 a          157 c          143 g          468 t
ORIGIN      
        1 acaaattatt atctaaaaac acttgataat caaggcttga acatggcaaa attctttttt
       61 tctttttatt cttcaatggt atttcaaccc acatttgttt agtataaata ttttcaaaaa
      121 atgttatatc ttgttctaaa aagttatttt gaacataata ttttgtaaat ttttctgctg
      181 aaaaatcgca ttttttttgt ttttgttgcg aaattccatc taaaaaatgc caaattattt
      241 gtgcagttag tgttgcagta agtacttttg ttgattcatt aaaaaaagta tcatgtaaac
      301 aaaatactcg aatatttgga gaatatcctg cataccaagc taattgacaa gattcttctg
      361 catacaaccc attgggattt gaatatattt gactgggcat atcactatat ctaattgcag
      421 acatatcaaa acttactatt tgagaatctc gaattaaagg ttctacttca agaatatttt
      481 gacgaacttt tcctaatctt accaatggaa aatattgtga tttaaaccac tcataggttt
      541 tctgtattga taaatattcc tgatatgcta tacttattaa tcgttgagtt tctgataaat
      601 ttacaagttt acttatataa ttagtattag aaaaatcttc agaatcaata tccaaatcta
      661 ttttagaatc aatacaacaa attgttggat actgtacttt ttttaaaatt tctttaccta
      721 tatagtataa cgattcttga gttcctccaa ttattattat gggtatttgt aattctttga
      781 gaatttttag aacttcttgt aaaataatat atgattcacg aactgtttta ccttgcttac
      841 aattaccaat atcaacaata gtaagtgatt ctgaggttgc acataacttt tctaaagaga
      901 cacgaaattc atctgctatt tgtaatgttg atgcaattgt cgaattcctg ttttctttgt
      961 atcctaatag aattgcatgt ggttttttat ttgtaatatc aattatatct tgtgaaaaat
     1021 gaatgtgaat ttttgaattg acatgcttat tatatactgt atttgaatgt tgttctaata
     1081 aatctatcaa ttccatatta tattattttt tttctccggc aatttcataa cattgttgta
     1141 acgttaa
//
LOCUS       DAOTOW010000756         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_102052_length_1141_cov_4.078269, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000756 DAOTOW010000000
VERSION     DAOTOW010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102052_length_1141_cov_4.078269"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1141
                     /gene="carA"
                     /locus_tag="P5243_11055"
     CDS             136..>1141
                     /gene="carA"
                     /locus_tag="P5243_11055"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRS20034.1"
                     /translation="MPDYKKVRLVLADGTEYHGYSFGYETSISGEVVFNTAMTGYVES
                     LTDPSYKGQILVATYPLIGNYGVPGKQVDEHNIPLFYESDQIQVSGLVVSDYSFEHSH
                     WNAEKSLADWFKEHKLPGIFGIDTRALTKKLRESGTMLGKILFDNQDVPMFDPGVENV
                     VAEVSVKEPVVYGNGSRKILLVDCGVKNNIIRCFLKRDAQVIRVPWDYDFSDMEYDGL
                     FISNGPGNPEVIQKTVDNIRKALEKRNNPVFGICMGNLLLGLASGARTYKLKYGHRSH
                     NQPVQQVGTHRCFITSQNHGYTVDIEQISGDWELLFVNNNDGTCEGIRHKTRPIFSAQ
                     F"
BASE COUNT          360 a          207 c          239 g          335 t
ORIGIN      
        1 ttttatatat caagtattat ttatgaccta aaccatgtaa aaacttgttg ataactcaat
       61 gttttttttt tgcctcgtaa aacctatacg tgtatatttg tgcaatttgt gtaataaata
      121 attaaatata tatacatgcc agattacaag aaggttaggc tagttttagc agatggaaca
      181 gagtatcatg gatactcatt tgggtacgaa acatcgattt cgggtgaggt agtgtttaac
      241 accgcaatga ccggttatgt tgaaagttta acagacccgt cgtacaaagg acaaatattg
      301 gttgccacat accctcttat tggcaactac ggtgttcccg gcaagcaggt tgatgaacac
      361 aatattccgt tattttatga gtcagaccag atacaagtat ctggtcttgt tgtatctgat
      421 tattcgtttg aacacagtca ttggaatgca gaaaagtctt tggcagattg gttcaaagaa
      481 cacaagcttc cgggtatttt tggaatagac acacgtgctc ttaccaaaaa attgcgcgaa
      541 agcggtacta tgttgggtaa aatattgttc gacaatcaag atgtaccaat gtttgacccc
      601 ggagtagaaa atgtggtagc cgaagtaagc gtaaaagaac cggttgtgta cggtaacggc
      661 tctcgcaaaa ttttgctcgt agactgtggt gtaaaaaaca atattatacg ctgcttcctt
      721 aaacgcgacg ctcaagtaat tcgagtaccg tgggattacg atttttccga tatggaatat
      781 gatggcttat tcatatcaaa tggtccgggt aatcccgaag taattcaaaa aactgtagac
      841 aatattcgca aagcgcttga aaaacgcaac aatccggtat ttggaatttg catgggcaat
      901 ttattgttag gcttagcaag tggcgcacgt acctataaac ttaaatatgg tcatcgtagc
      961 cacaaccaac cggtacaaca agttggtact caccgttgtt ttattacatc gcaaaatcat
     1021 ggttacaccg tagatataga acaaatatcg ggcgattggg aattgttgtt tgtaaacaac
     1081 aacgacggca catgcgaggg aattagacac aaaactcgtc ccatattctc ggcacagttc
     1141 c
//
LOCUS       DAOTOW010000757         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_102103_length_1141_cov_2.252302, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000757 DAOTOW010000000
VERSION     DAOTOW010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102103_length_1141_cov_2.252302"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..895
                     /locus_tag="P5243_11060"
     CDS             218..895
                     /locus_tag="P5243_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20035.1"
                     /translation="MKILSIFFVLLCVGVACNSDISYSPQISIFVNTSSVNSVEEDSI
                     VHVPCVISAYDKSVLKRIQMKHNKTIVYDTLISNTTDFSHVFTYSFLGLQGLQKLTLY
                     AFDNQDLYSEQTIQYFVKELQSPSLILDSTNIYKDTIIHKLQTAEFSILCKQGERMLD
                     SLYVYHNSIKQTSYPNIQNSIFTDSITRIPYTCNFSKTGIYIVRFRLLDKAKKTTQKQ
                     VKITVVE"
BASE COUNT          425 a          135 c          164 g          417 t
ORIGIN      
        1 ttcagctcgt atttatgatt tagaagctgg tgatgtagta gcattcaaaa cagctgccgg
       61 taaatatggt gttttaaaag ttgtaaaagt tcaaggaact tataacaatg gtgactacat
      121 tgaaataaac gtaaaagttc aaaaataatc tttagattat ataattctta taatgccgac
      181 ttgaataaca agtcggcatt ttttttatat ttgtggtatg aaaattttaa gtatcttctt
      241 tgtattacta tgtgtggggg tagcttgtaa ttccgatata agttattctc ctcaaatttc
      301 tatttttgta aatacatctt ctgttaattc tgtagaagaa gatagtattg tacatgtacc
      361 atgtgtaatt tctgcatatg ataaatcagt tttgaaacgt attcaaatga agcataataa
      421 aactattgtt tatgatacat taatttctaa tactacagac ttttcacatg tttttacata
      481 ttcattttta ggattacaag gtttgcaaaa gttaacatta tatgcttttg ataatcaaga
      541 tttgtattct gaacaaacaa ttcaatattt tgtgaaagaa ttgcaaagcc caagtcttat
      601 attagattct accaatatat ataaagatac aataattcat aagttacaaa cagcagagtt
      661 ttcaattctt tgcaaacaag gagagcgaat gttggattca ttatatgttt atcataattc
      721 aataaaacaa accagttatc caaatattca aaatagtata tttacagata gcattacaag
      781 aattccttat acatgtaatt tttctaaaac aggtatatat atagtgcggt ttcgtttgtt
      841 agataaagca aagaaaacga ctcaaaaaca agttaaaatt acagttgttg aataaatata
      901 aaacttacat ggttaatatt aaaagaaatc aaattgtaca attaatgcat gtggggtcta
      961 tcatatttta gaaaataaaa attgtattat aatgaaagtt ctatcatatt attctctgtt
     1021 ttttcttgtt ttttgtacta tggtattgta ttcatgttcc tcaaaaaaaa ataaagaatt
     1081 aagccccgat gatattaaac ggttacaaga aaaatctatt gaagtaaata gagaagttgt
     1141 a
//
LOCUS       DAOTOW010000758         1126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_104261_length_1126_cov_2.913165, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000758 DAOTOW010000000
VERSION     DAOTOW010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104261_length_1126_cov_2.913165"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="P5243_11065"
     CDS             <1..233
                     /locus_tag="P5243_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20036.1"
                     /translation="DQYQRYQIQLYHISKDFYEYMQSSYLYEKNRYPEFSFNGITPYN
                     LYSNTSNNHGVFMSSAVSITDTIRIYNNPSLF"
     gene            245..>1126
                     /locus_tag="P5243_11070"
     CDS             245..>1126
                     /locus_tag="P5243_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS20037.1"
                     /translation="MTRYIQHIFFILLIGVSHTALSQHNIACTGTVRDAQSGELLIGA
                     SIYQVGSNNAVYTNERGLFTIFVQTPARIECSYVGYALASYTITSNTDTTLTILLASN
                     NIIETVQVVAAQTTKANTSSLTMRELTAIPALGGKPDVLKTAQLLPGIQAQNEGSSLL
                     TVRGGDPGQNLYLIDNVPLIYVNHLGGFTSVFNPDVINALDIYKGSFPSKYGGKLSSI
                     LNITQRSGNAGTIKGNYTVGLSDLSFTLEGPTKIKNTQFIISGRKTVLPDVFMYAASS
                     ISRGNSVKLSYGFHDINA"
BASE COUNT          371 a          235 c          195 g          325 t
ORIGIN      
        1 atgatcaata tcaacgctat caaattcagt tataccatat ttcaaaagat ttttatgaat
       61 acatgcaaag ttcctatttg tacgaaaaaa atcgctatcc cgaatttagt ttcaatggta
      121 taaccccata caatttgtat tcaaatacat caaacaatca tggtgtattc atgtcttcgg
      181 cagtaagcat aaccgatact attcgcatat acaacaaccc tagtttattt taaccccggc
      241 atcaatgaca cgttatattc aacatatatt ttttatttta ttaataggag tttcacacac
      301 tgccctatca caacacaata ttgcatgcac cggaactgta cgcgatgctc aaagcggcga
      361 attactcata ggtgcatcta tataccaagt aggctccaac aatgcggtat atactaacga
      421 gcgtggttta tttacaattt ttgtacaaac acctgcacgt attgagtgtt cgtatgttgg
      481 ctatgctttg gcaagctata ccattacaag taatacagat acaactctca caatacttct
      541 tgcaagcaac aatataatag aaaccgtgca agtagtggct gcacaaacca caaaagcaaa
      601 cacatcaagc ctaacaatgc gtgaacttac tgccattccg gcattaggag gcaaacccga
      661 tgtgcttaaa actgcacaat tactacccgg tatacaggca caaaacgaag gttcgagttt
      721 gctgactgtt cgtggcggcg acccggggca aaatttatat cttatagaca atgttccact
      781 gatatatgta aatcacttgg gcggatttac ctcggttttt aatcccgatg ttataaatgc
      841 attagatatt tacaaaggta gttttccgtc gaaatatgga ggcaaattgt cgtctatact
      901 caacattaca caacgctcgg gcaatgccgg cacgattaaa ggtaattata ctgtaggatt
      961 aagcgacctc tcgtttaccc tcgaaggtcc tacaaaaata aaaaatacac aattcatcat
     1021 ttcgggtcgc aaaacagtgt tgcccgatgt gtttatgtat gcagcatcaa gtatatcgcg
     1081 aggaaatagc gtaaaattga gctatggatt tcatgatatt aatgca
//
LOCUS       DAOTOW010000759         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_104555_length_1124_cov_2.753040, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000759 DAOTOW010000000
VERSION     DAOTOW010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104555_length_1124_cov_2.753040"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1124)
                     /locus_tag="P5243_11075"
     CDS             complement(<1..>1124)
                     /locus_tag="P5243_11075"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS20038.1"
                     /translation="FNSKGQFPWEIKYLGTQIVYPEFSVSNNMGVDSALVYFYNNSVN
                     AISYAWDFNNDGVVDSNVESPAFVYMQPGIYTVKLQITGYEGAQLVTKTIVKPDYILV
                     QKSPQTGTYCQLAKQAVIGNNTMSMVDGTTQWFKYTSDFEGKLVIQTCNSNYPLGIYI
                     YDMYDDCNNHRVYGSNTMCGNKTTQYLDVVPGKTYYFNMMIYSYVSGINTVNFELIKQ
                     LPQPGDLCGLPIILQSSSIQSIQKPVGNIGSANVEKWYSFTAPYNGVAEINTCINQDL
                     GMINAELFNTCNMNDYNNYPDYDYRECQTSNYGYSSGAKVVQMVKDQTILIKVSGSVS
                     QVAQWQFVYRDYYAGEVCTKPLLAKQGVNSNPSGLTEIFYT"
BASE COUNT          384 a          201 c          147 g          392 t
ORIGIN      
        1 gtataaaata tttctgttaa accggaagga tttgaattta ccccttgttt agcaagtaat
       61 ggttttgtac aaacttcgcc tgcatagtaa tctctataca caaattgcca ttgtgcaact
      121 tggcttacac ttccactgac tttaatcaaa attgtttgat cttttaccat ttgaacaact
      181 ttggcccccg atgaataccc ataattacta gtttgacact ccctataatc ataatcagga
      241 taattattat agtcattcat attacaagta ttaaacaatt cggcatttat cattcccaaa
      301 tcttgattaa tacaagtatt gatttcggct acaccattat aaggagctgt aaatgaatac
      361 catttttcta cattagcact gcctatatta cccacaggtt tttgaataga ttgtattgaa
      421 gaagattgaa gtataatagg caaaccacat aaatcaccgg gctgaggtaa ttgtttaatt
      481 aattcaaaat taactgtgtt tattcccgat acatatgaat atatcatcat attaaaataa
      541 tatgttttgc caggaacaac atctaagtat tgtgtagttt tgttgccaca cattgtattt
      601 gaaccataaa ctctatgatt gttacaatca tcatacatat cataaatata aatacctaac
      661 ggataatttg aattacatgt ttgaattact aattttcctt caaagtcaga ggtatattta
      721 aaccattgag tagttccatc aaccatactc attgtattat taccaataac agcttgtttt
      781 gctaattgac aatacgtacc ggtttgtggt gatttttgaa ctaaaatata atctggtttt
      841 actattgttt tagtaaccaa ttgagcacct tcataaccgg taatttgaag tttcacagta
      901 tatatacccg gctgcatata tacaaaagcc ggactttcaa catttgaatc aacaactcca
      961 tcattattaa aatcccatgc atacgatatt gcattaacag aattattata gaaataaaca
     1021 agagccgaat caactcccat gttattactc acactaaatt caggatatac aatttgagtt
     1081 cccaaatatt ttatttccca tgggaattga ccttttgaat taaa
//
LOCUS       DAOTOW010000760         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_104765_length_1123_cov_2.101124, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000760 DAOTOW010000000
VERSION     DAOTOW010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104765_length_1123_cov_2.101124"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..957)
                     /locus_tag="P5243_11080"
     CDS             complement(10..957)
                     /locus_tag="P5243_11080"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRS20039.1"
                     /translation="MEFIQKYIFTFLRILIGFVFIISALLKLAPIAPFENILISFKIS
                     NWLYAPYIARCIISFELAIGFFIIANVYTKNSLRVALYTLIIFSLFLMYIYSIGHSQQ
                     NCGCLGEVIELKPIESILKNCVLIAVILYCLKNHHKLQENEFKKIMPFIAGIIIIIAG
                     AIPYLYNMPTRANEPSNYIIKPGMKISHQSIQQCILNTGDTVNFTHNKHILVFASLTC
                     NSCKYAISKLENIHKYNQNFNISIIFLETPHKDSLLTQLIEKTGLQTIPYSFLSPEDF
                     FKTSHNKLPFIVFVENGEIKNLRNYNDLYIGDIFTFFKQ"
BASE COUNT          418 a          128 c          179 g          398 t
ORIGIN      
        1 cggcattttt tattgtttaa aaaaagtaaa aatatcacca atatataaat cattgtagtt
       61 acgtaaattt ttaatttcac cattttcaac aaatacaata aaaggtaatt tattgtgtga
      121 tgttttgaaa aagtcttcgg gtgataaaaa tgaatatgga atggtttgta atccggtttt
      181 ttctatcagt tgagtaagta aagagtcttt gtgtggcgtt tctagaaaaa ttattgaaat
      241 gttgaaattt tgattgtatt tgtgaatgtt ttctaatttt gatatagcat atttacaact
      301 gttgcatgta agcgaagcaa atacaagtat atgtttattg tgcgtaaagt ttactgtgtc
      361 accggtatta agaatacatt gttgtattga ttgatgagag attttcattc cgggtttgat
      421 tatataatta ctaggttcat ttgcacgagt aggcatgtta tacaaataag gtatagctcc
      481 tgcaatgatt ataataattc ctgcaatgaa aggcatgatt tttttaaatt cattttcttg
      541 tagtttatga tgatttttca aacaatacag aataacagct ataagaacac aatttttaag
      601 aatagattca atcggtttaa gttcgattac ttcacctaaa caaccacagt tttgttggga
      661 gtgaccaatg gaatatatat acataagaaa cagtgaaaat ataataagag tatacaatgc
      721 aactcttaat gaatttttgg tatacacatt tgcaataata aaaaaaccaa ttgcaagttc
      781 aaagctaatg atacatcgag ctatataggg agcatataac caattggaaa ttttaaaaga
      841 aattaggatg ttttcaaatg gtgcaattgg agctaattta agcaatgcac taattataaa
      901 aacaaaacct ataagaattc taagaaacgt aaatatatat ttttgtataa attccatgat
      961 gtaaatataa taaaaaaaga ggttgacaat tattatgtgt caacctctaa ttatagtata
     1021 tatgtaattt taaaaaacaa tacctaattt tgcaactcct agaggaagaa taccggtata
     1081 tcccatattt aaaatgccat catgattgtt gatgtatgat ttg
//
LOCUS       DAOTOW010000761         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_104928_length_1122_cov_2.002812, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000761 DAOTOW010000000
VERSION     DAOTOW010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104928_length_1122_cov_2.002812"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5243_11085"
     CDS             <1..893
                     /locus_tag="P5243_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRS20040.1"
                     /translation="YAQQKPIPTQYMNDKYFVNSAYAGTTNNATASINAKQYLNGIEG
                     APTTQYLYVQYPLLANFMGVGLKVMHDQIGVTSQNNALITYAYQIGILGGNLSFALDA
                     GLYNAKTRFSDLIISDDAADQAIPMNNETIMLPDASFGMFFTFQNLYAGYNAYNLMGG
                     KTSYTSTGYIGKEVLSKYHYIIGGYTIKPNKELQIEPILLCKVAQAAPIQVDVSLYTQ
                     YTKLLSGAVTYSTQRGLSFMVMAGFKEGQYKIGYSYDMSIPGNRASFLHAGHEIMFKY
                     SLELTPPASQKDFNPIYYLN"
BASE COUNT          415 a          181 c          174 g          352 t
ORIGIN      
        1 tatatgctca gcaaaaacct atacctacac aatatatgaa cgacaaatat tttgtgaatt
       61 ctgcttatgc cggaactaca aataatgcaa cagcttcaat caatgcaaaa caatacctca
      121 acggtattga aggagctcct actactcaat acttatatgt acaatatccc cttttggcaa
      181 attttatggg agttggttta aaagtaatgc atgaccaaat aggggtaaca tcacaaaata
      241 atgcacttat aacctatgct taccaaattg gtatattagg aggtaatctt tcatttgctc
      301 ttgatgccgg tttatataat gcaaaaacac gtttttctga tttgataatt tctgatgatg
      361 ctgccgacca agccatacca atgaataatg aaactattat gttacccgat gcttcatttg
      421 gaatgttttt tacatttcaa aatctgtatg caggatataa cgcatataat ttaatgggag
      481 gtaaaacttc ctatacatct acaggatata ttgggaaaga agttttatca aaatatcact
      541 acattattgg aggatacaca ataaaaccca ataaagagtt acaaatagaa ccaattctat
      601 tatgtaaagt tgcacaagca gctcctattc aagtagatgt atcattgtat acacaataca
      661 caaaattact tagcggtgct gttacttaca gtacccaacg aggtttatct ttcatggtta
      721 tggcaggttt taaagaaggt caatataaaa taggctattc atatgatatg tcaatacccg
      781 gtaatagagc ttcattttta catgcagggc acgaaattat gtttaaatat tcattagagt
      841 taactccacc tgcaagtcaa aaagatttta accctattta ttatttgaat taatgatgaa
      901 taaatatttc atacatattg tgttatgtag tattatttgc atagttttga tgcaaacaca
      961 agatatgtat gctcaaaaaa atattgtaaa agctaatatg tattataaca gccagttata
     1021 tgcacaagca atagaatatt ttgtaaaaga agtggctgca aacgataaag aaacaaaacg
     1081 agatgctatg ataaaactag catattgcta caaacaaacc gg
//
LOCUS       DAOTOW010000762         1118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_105432_length_1118_cov_3.951082, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000762 DAOTOW010000000
VERSION     DAOTOW010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105432_length_1118_cov_3.951082"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="P5243_11090"
     CDS             complement(<1..109)
                     /locus_tag="P5243_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRS20041.1"
                     /translation="MTLDSFSSFFFVGIAGTGMSAIAQYLQGVGKHVSGS"
     gene            complement(239..>1118)
                     /locus_tag="P5243_11095"
     CDS             complement(239..>1118)
                     /locus_tag="P5243_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS20042.1"
                     /translation="KHEVFFIVKSKKIIPISLSISLVNDEQNNELGWVCMFHDISERK
                     TFEKNLIIAKENAEQADKMKSMFLAKMSHEIRTPLNSIIGFSDLLEDDDITKDERDRY
                     VQIIRKNGEKLLAFIDNLLDISSLETQKIHIEKNSCSPQELMQDLYDEFLAQQKKQKK
                     DAITFKMHNTTAPDFYFMTDQKRLYQCLYNILENAFKFTTEGEITIVCKINEIGNYID
                     FIVQDTGVGIVPEIENRIFDTFQNIEADSSEKTGGLGLGLAISKNIVKLLNGSILLKT
                     KPNIGSMFTVRLPINN"
BASE COUNT          370 a          201 c          154 g          393 t
ORIGIN      
        1 cagaacccga aacatgctta cctacccctt gtaaatactg cgctatggcg ctcatacctg
       61 ttccggcaat acccacaaaa aagaatgatg aaaacgaatc taatgtcata tagtatagta
      121 attatcaatg attactgttt aattgctcat gaaaacatta aagaaaaaag tcttaattgc
      181 aaattcaaaa ctacaataaa tttcttgaaa aatttacaaa aagcagcctt gtaatctact
      241 aattgttgat aggtaaacgc acagtaaaca ttgaaccaat atttggttta gttttaagta
      301 agatagaacc attaagcagt tttacaatat tttttgaaat tgcaagacct aaacccaaac
      361 caccggtttt ttctgacgaa tcggcttcaa tattttgaaa cgtatcaaaa attctatttt
      421 ctatttcagg cactatacct acgccggtat cttgcacaat aaagtcaata taattaccaa
      481 tttcattgat tttgcaaaca atggtaattt ctccttcggt agtaaattta aaggcatttt
      541 ccaaaatatt gtacaaacac tgatagagtc gtttttggtc ggtcataaaa taaaaatcgg
      601 gagcagtggt attgtgcatt ttaaacgtaa ttgcatcttt tttctgcttt ttctgttgtg
      661 ccaaaaattc atcgtacaaa tcttgcatca attcttgagg gctacaacta tttttttcta
      721 tatgaatctt ctgcgtttcg agcgatgaaa tatcaagtaa attgtcaata aacgccaaca
      781 atttttcacc attttttcta ataatttgaa catatctgtc tcgttcatct ttggttatgt
      841 cgtcatcttc caacaaatca gaaaaaccta tgatagaatt gagcggagta cgaatttcgt
      901 gactcatttt tgccaaaaac atagatttca ttttgtcggc ttgctcagca ttttcttttg
      961 caataatcag atttttttca aatgttttgc gttcgcttat atcatgaaac atacacaccc
     1021 agcctaattc attattttgt tcgtcgttta ccaacgaaat tgacaatgat ataggaatta
     1081 tttttttact ttttactata aaaaacacct catgtttt
//
LOCUS       DAOTOW010000763         1118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_105493_length_1118_cov_2.098777, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000763 DAOTOW010000000
VERSION     DAOTOW010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105493_length_1118_cov_2.098777"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>1118
                     /locus_tag="P5243_11100"
     CDS             153..>1118
                     /locus_tag="P5243_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRS20043.1"
                     /translation="MNRSINITQPNTMPHLTSMVWDYLDQLQSASNGTFTSYYNYDSE
                     GNRTRKVVVKNNIIETRYYINGYELYRKEINGVVDFERKTINISDDEKVFVRIEQKTG
                     ENPIVRYQYDNHLGSACLELDNTGQIISYEEYHPFGTTSYKSGRSQTEVSLKRYKYCG
                     KERDEETGLYYYGMRYYAAWICRFVNVDPLQFKYPYYTPYQYAGNKPITYIDLDGGEE
                     KDSQTYTVQSGDDPTKIAQKFGISIWDLAKWNQGHQEGGGFFTDIGDGNYKDYWVTGK
                     GTSWSIKPGDVLNIVDGKQSTHSTNVIDGKFEYNGSMYPMGYPQGG"
BASE COUNT          418 a          158 c          221 g          321 t
ORIGIN      
        1 attctatgga aagtaaatta tagaactaaa taacctaaag ccttgcagag atgggtagtg
       61 taccatatgg agcttttgta aaatttaatt ttgatagtaa aagtttatgt taataaattt
      121 ttttataatt ttgaaaataa aataattatg ctttgaacag atcaataaac ataacacaac
      181 ctaataccat gccccattta acaagcatgg tgtgggatta tttagaccaa ttacagagtg
      241 caagtaatgg aacatttaca agttattata actacgactc cgaaggtaat agaactcgaa
      301 aagttgttgt aaaaaacaat attattgaaa ctcgatacta tattaatggg tatgaattat
      361 atagaaaaga gattaatggc gttgtggatt ttgaacgtaa aacaataaat atttccgatg
      421 atgaaaaagt gtttgttcgt attgagcaaa aaaccggtga aaatcctatt gtaagatatc
      481 aatacgacaa ccatctcggt tcagcctgct tagagttaga caataccgga caaattattt
      541 cctacgaaga ataccatcct tttggcacaa caagttataa aagtggacga agccaaaccg
      601 aagtttcttt aaaacgctac aaatactgtg gtaaagaacg cgatgaagaa accggattat
      661 attactatgg catgcgttat tacgctgctt ggatatgccg ctttgtgaac gtggatccgc
      721 tgcagtttaa atacccttat tacacgcctt atcaatatgc agggaataaa ccaattacat
      781 atattgattt ggatggaggg gaagagaaag attcacagac atatacagta caaagtggag
      841 atgatccgac aaaaattgca caaaaatttg gaattagtat ttgggattta gcaaaatgga
      901 atcaaggaca tcaagaagga ggaggttttt ttacggatat aggagacggt aattataagg
      961 attattgggt aacaggaaaa ggaacgtcat ggtccataaa gccgggagat gtattaaaca
     1021 ttgtcgatgg aaaacaatct acccattcaa ctaatgtaat tgatggaaaa tttgaatata
     1081 atggttcgat gtatcctatg ggctatccac aaggtgga
//
LOCUS       DAOTOW010000764         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_105901_length_1115_cov_2.982075, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000764 DAOTOW010000000
VERSION     DAOTOW010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105901_length_1115_cov_2.982075"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..305
                     /locus_tag="P5243_11105"
     CDS             81..305
                     /locus_tag="P5243_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20044.1"
                     /translation="MEQIVFLWVWGGGFKTRYGNGPHKFSATGVGLLARGDFYYPYGI
                     IWQENGVWQTGRIDSVYIKSFETYTDTIYY"
     gene            complement(607..>1115)
                     /locus_tag="P5243_11110"
     CDS             complement(607..>1115)
                     /locus_tag="P5243_11110"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20045.1"
                     /translation="ITNTSKQILYARIQLQGIALMKPQAANEENLKLSVNYYTMDGKL
                     LNPQSLKQGTDFYAEVTITHPGFKSTYKDMAVQHMFPSGWEILNTRMDEIDNNAANNS
                     SMRYQDIRDDRVYSYFDLQPYEIKTYRILLTAAYLGKFFLPSVYCEAMYDNTIHARSQ
                     GIWVEVKR"
BASE COUNT          335 a          174 c          224 g          382 t
ORIGIN      
        1 aactatggat gcctacgctt ggtttaatcc tattcttaaa ggaagtaatt gcattaatac
       61 tgattctgtt ttgattttag gtggaacaga ttgtattcct atgggtatgg ggggggggat
      121 ttaaaactcg ttatggtaat ggtcctcata aattttcagc aacaggagtc ggattattag
      181 cgcgaggtga tttttattat ccgtatggta taatttggca agaaaatgga gtatggcaaa
      241 caggaagaat tgatagtgtg tatataaaat catttgaaac atatacagat acgatttatt
      301 attaaaaata aatgttcaga gtgttaataa agtgtataat cttattccga caatagtatt
      361 ccttttgaat cttctacaac tgtagaatat ggcgtgcgaa acagggggtg aggcaaacag
      421 aaataaaacc atagcaacac tgtaaacccc aaggtagata ggtatacctt ggggtgcaaa
      481 gtgtgttgac tatattttgt gtttttaatt tcaagatcta aaaagtctta atgtccctat
      541 actgtaagcc aaattattct ttactacgta actcaaaatt aagaacttaa aactcaaaac
      601 taatttttac cgttttacct caacccaaat tccttgtgag cgtgcatgta ttgtattgtc
      661 gtacatagcc tcgcaatata ccgacggtag aaagaatttg cctagatatg ctgccgtaag
      721 aagaattcta taggttttaa tttcatatgg ttgtaaatca aaatatgaat acactctatc
      781 gtcgcgaata tcttgatatc gcatgctgct gttgtttgcg gcattgttat caatttcgtc
      841 catgcgcgtg ttgagtattt cccaaccact cgggaacata tgctgtactg ccatgtcttt
      901 gtatgttgac ttaaatccgg ggtgtgtaat ggtaacttcg gcatagaaat cagttccttg
      961 tttcagcgat tgggggttca gtaacttgcc atccattgta taataattaa ccgataattt
     1021 taagttttct tcatttgctg cctgtggttt cataagtgca attccttgca gttgaatacg
     1081 agcatatagt atttgttttg aagtattggt aatgc
//
LOCUS       DAOTOW010000765         1114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_106056_length_1114_cov_3.301228, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000765 DAOTOW010000000
VERSION     DAOTOW010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106056_length_1114_cov_3.301228"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..944)
                     /locus_tag="P5243_11115"
     CDS             complement(210..944)
                     /locus_tag="P5243_11115"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRS20046.1"
                     /translation="MEIYNNILIFDTLSSTNEHAFKIIESNQVKEFTTIYTPNQTRGK
                     GQRGNSWLSEPGLNCLFSIILFPKKIPANNQFILSQAVSVGLLHALQKYCNNIYIKWP
                     NDIYINNKKIAGILIENILLGQNISKSVVGIGLNCNQTIFDESLPNPTSLALETNKKY
                     DCIEIINECIESIEKTYVNIDHAKNEIENTYHTALYKRNILAQYKDSNGIFDAKIIGV
                     EPTGKLLLQDSYNQLRTYNFKEIEFL"
BASE COUNT          368 a          134 c          155 g          457 t
ORIGIN      
        1 agaacaccga tacatcctaa taataataaa ataattaaaa tgaaaaatac ataaatttta
       61 gagtttgctt tttgatttgt atccataata gttaaaatat ataagttctt ttgaaatttg
      121 gaacaaaaat aattaattca attggttttt tatcaatgtt ttgaattttt tatttaaaca
      181 gtctattaac taaatttatt atctcttttt tacaaaaatt caatttcttt aaagttgtat
      241 gttctcagtt ggttatatga gtcttgtaat aataatttcc ctgtaggttc tactcctata
      301 atttttgcgt caaatattcc attactgtct ttatactgag ctaaaatatt tcttttatat
      361 aaagcagtgt gatatgtgtt ttctatttca ttttttgcat ggtctatatt tacatatgtt
      421 ttttcaatag attcaataca ttcgttaatt atttctatac aatcatattt tttgttagtt
      481 tcaagagcca gtgaagttgg attaggtaat gattcgtcaa atatcgtctg attgcaattg
      541 agtccaatac ctacaacact tttggaaata ttttgaccta acagaatatt ttcaataagt
      601 attccggcaa tttttttgtt atttatatat atatcattag gccatttaat gtatatgttg
      661 ttacaatatt tttgtaatgc atgtaataag cctaccgaaa cagcttgaga aagaataaat
      721 tggttgtttg caggaatttt ttttggaaaa agaataattg aaaataaaca atttaagccg
      781 ggttccgaaa gccatgaatt gccgcgttgt ccttttccac gagtttgatt gggggtgtaa
      841 attgtagtaa attcttttac ttgattactt tcaataattt tgaatgcatg ttcgttagtt
      901 gatgaaagtg tatcgaaaat aagaatattg ttgtatattt ccacgcttaa tttttatata
      961 tatgaaacgt tatatcgtat ttcttttagt tattatatct tatgcactgc aagcacaaga
     1021 agttagttta caaggtaaaa ttacaaatta ttccggtaaa gattcgctaa aattattaat
     1081 ttattcaagt aatacgccgg tagatatatc aatt
//
LOCUS       DAOTOW010000766         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_106241_length_1113_cov_2.798677, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000766 DAOTOW010000000
VERSION     DAOTOW010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106241_length_1113_cov_2.798677"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="P5243_11120"
     CDS             <1..>1113
                     /locus_tag="P5243_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS20047.1"
                     /translation="VGQFNYMLISSENPPTIINSGVTTNTVYTFSNLSENISYTILVD
                     NVLTSQRIGLDGAVLTAIPYFANTSRIKGAGCRGEAVGTVNVEVTGGVRPYSYSWQNG
                     MGGYPNDSVIYNVGPDVYIVKITDAVGCEINSTTTTIVPEDVVVNTTIQKQVECKGQS
                     DARVSAIAANGTGAYTYTWSDGYVGALNTQLPAGNHYVVASDEISCTDTSYFTITEPA
                     NKIAIHLDSKEDLKCKDIPTGKANLSTTYGISPLTYTWSDGGSGASRTNLSATSYRVI
                     VSDSKQCKDSVDFSLTQPATAIKGSVDNFTMPLCFGNKNGTAQISATGGTPGYTYLWD
                     DPNNDSTKNNTTRTDLGSRNYTITFTDANGCTDIVE"
BASE COUNT          378 a          187 c          226 g          322 t
ORIGIN      
        1 tgtaggtcaa tttaattata tgttgataag ctctgaaaat cctcctacta ttataaattc
       61 aggagtaact actaataccg tttatacttt tagtaacttg tctgagaata tatcttatac
      121 tattttagta gataacgttt taacatctca aagaatagga ttagatggtg ctgtattaac
      181 agcaatcccc tattttgcaa atacatcgcg aatcaaaggt gccggttgta gaggcgaagc
      241 ggtaggtacg gttaacgtgg aagttacggg tggagtacgt ccctatagtt actcatggca
      301 gaacggtatg ggagggtacc ccaatgattc tgtgatttat aatgttggtc ctgatgtata
      361 tattgtaaaa attaccgatg cggtagggtg cgaaataaat tctacaacaa ctacaattgt
      421 tccggaagat gtggttgtga ataccactat tcaaaaacaa gtagaatgta aaggacaaag
      481 tgatgcccgt gtaagtgcta ttgctgcaaa tggaaccgga gcatatacct atacatggag
      541 tgatggttat gtgggagctc ttaacacgca attacctgcc ggaaatcatt atgtagtggc
      601 aagtgatgaa atttcatgta ccgatacatc atattttact attaccgaac ctgccaacaa
      661 aattgcaata catcttgata gtaaagaaga cttaaaatgt aaagatattc ctaccggaaa
      721 agcaaatcta tcaacaacat atggaattag tccacttacc tatacatgga gtgatggcgg
      781 ctcaggagca tcgcgaacaa atttatcggc tacttcatac agggtaatag taagcgacag
      841 taaacaatgt aaagatagtg ttgatttttc actcacgcaa cctgcaacag ctatcaaagg
      901 ttctgttgat aattttacta tgccactgtg ttttggaaat aaaaacggaa cagctcaaat
      961 tagcgcaaca gggggaactc ctggatatac gtatttatgg gacgacccaa ataatgattc
     1021 aacaaaaaat aatacaacca gaacagattt aggtagcaga aattatacta ttacatttac
     1081 tgatgctaat ggttgtaccg atattgtaga att
//
LOCUS       DAOTOW010000767         1107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_107204_length_1107_cov_3.078897, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000767 DAOTOW010000000
VERSION     DAOTOW010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107204_length_1107_cov_3.078897"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="P5243_11125"
     CDS             <1..810
                     /locus_tag="P5243_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRS20048.1"
                     /translation="QAVVATGRLSSTNPNLQNIPIKTAKGREIRKAFIPLHKEHVFIS
                     ADYSQIELRVIAHMSGDSNFIQAFKNGEDIHSATAAKIFNTPITEVTQEQRRQAKSAN
                     FAITYGSSAFGLSQTLSISRKDAQFLIDGYFAAYPQVKQFMDSQIRIAQEKGYVETMF
                     GRRRYVPDIHSQNATVRGFAERNAINAPIQGTAADIMKIAMIRIHSMIQKQSLQSKLI
                     MQVHDELNFSVPISEVDAMKEIIRIGMEQAVTLSVPLIADIGVGNNWLEAH"
BASE COUNT          400 a          184 c          178 g          345 t
ORIGIN      
        1 caggctgttg ttgctaccgg tcgattgagc tctacaaacc caaatttgca gaatattcct
       61 attaaaactg ccaaaggacg agaaatcaga aaagccttta ttcctttaca taaagaacat
      121 gtatttatat cggctgatta ctcacaaata gagttgcgag taatagctca catgagtggt
      181 gacagcaatt ttatacaagc ttttaaaaat ggtgaagata tacactctgc cactgcggct
      241 aaaatattta atactccaat aacagaggtt acccaagaac aacgccgaca agctaaatcg
      301 gcaaattttg ccattacata tggttcaagt gcatttgggt tgtcacaaac gctttctatt
      361 tcacgaaaag atgctcaatt tcttatagac ggatactttg ctgcttatcc acaagtaaaa
      421 caatttatgg attcacaaat tcgtatagca caagaaaaag gatatgtaga aactatgttt
      481 ggacgccgca gatatgttcc cgatattcat tcacaaaatg ctactgtacg aggctttgct
      541 gaacgaaatg ctataaatgc acctatacaa ggtactgctg ccgacattat gaagattgcc
      601 atgattcgaa tacattctat gattcaaaaa caatccttac aatcaaaact cataatgcaa
      661 gtacatgatg agttaaactt ttcagttcca atttctgaag ttgacgcaat gaaagaaata
      721 atacgaatag ggatggaaca agcggtaaca ctttcagttc cattaatcgc cgatattggt
      781 gtaggaaata attggcttga agcacattaa aaattacttt atattaattt actaaacaat
      841 ttactaaaat cagttttaaa aaatttatga ggggatagtg tattttttac aattttatac
      901 tatatttgat tgaattttat tgtatcgtag taaaaacaaa acaataatta aaactatact
      961 tgtatgaaat attccacaga aaaactctca atttttgaaa aagttggata tagtttaggt
     1021 gatttagcag caaatttagt gtttcaaaca ctaattacat acttagccta tttttatact
     1081 gatatttatg gcttgaacaa taatgac
//
LOCUS       DAOTOW010000768         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_107520_length_1105_cov_2.802857, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000768 DAOTOW010000000
VERSION     DAOTOW010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107520_length_1105_cov_2.802857"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1105
                     /locus_tag="P5243_11130"
     CDS             <1..>1105
                     /locus_tag="P5243_11130"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20049.1"
                     /translation="STVYIRIKNSDGTYNVFSPFYTPTLKPLDKFECHIGLSYTTWIV
                     EAFGVKSEITVFVPQGSQTVLQDIKVTNISGKDLEIDVIPVYEFTHFDALKQLTNADW
                     VPQTMTLNGHWESTGHVILEQYAFMKKQYAVNLVTCDRPVSSFEGDRRVFLGDFEYGS
                     FANPLSLQKPELSNSECVRGDNVAVLLIKFGTMPNGSTQRTVTQLTQKQSIAQAQEEI
                     QKYRNLANVDKAFSDLAAWWDNYLQVMTIESPDASFNSMINVHNPRQCHTTKNWSRYL
                     SLYQLGFGSSRGIGFRDSSQDLLGAMSHMPEEARVLAENLLSVQLPNGSAMHQYFPST
                     MEANEGDSREEPHLPDYYGDDHLWIVQTVSQYL"
BASE COUNT          379 a          197 c          187 g          342 t
ORIGIN      
        1 gttcaacagt atatattcga attaaaaaca gtgatggtac ttacaatgtt ttttcacctt
       61 tttacacacc aacactcaaa ccacttgata aatttgaatg ccatatagga ctttcataca
      121 caacttggat tgtagaagca tttggagtaa aatctgaaat tacagttttt gtaccacaag
      181 gttcacaaac tgtattacaa gatataaaag ttactaatat ttcgggtaaa gaccttgaaa
      241 tagatgtaat tccggtatat gaatttacac attttgatgc tctaaaacaa ttaacaaatg
      301 ccgattgggt tcctcaaaca atgacactaa acggacattg ggaatcaaca ggtcatgtta
      361 tacttgagca atatgcattc atgaaaaaac aatatgctgt aaatttagtt acctgcgacc
      421 gcccggtatc ttcatttgaa ggtgatagac gtgtattttt aggtgatttt gaatatggtt
      481 catttgcaaa tcctctttca ttacaaaagc cggaattatc taattcagaa tgtgtacgag
      541 gcgacaatgt tgctgtatta cttattaaat ttggtactat gccgaatggt tcaacacaaa
      601 gaactgttac acaacttaca caaaaacaat caattgctca agctcaagag gaaatacaaa
      661 aatatagaaa tctggcaaat gttgataaag cattttctga tttagctgca tggtgggata
      721 attatttaca agtaatgact attgaatcac ctgatgcttc atttaattca atgattaatg
      781 tccacaatcc tcgacagtgt catactacca aaaactggtc acgttatcta tcattatatc
      841 aactaggatt tggaagttca cgcggtatag gattccgtga ctcttcacaa gatttacttg
      901 gagcaatgtc tcacatgccg gaagaagcac gtgtattggc agaaaattta ctttctgtgc
      961 aattaccaaa tggttcagct atgcaccaat attttccatc aacaatggaa gctaacgagg
     1021 gtgactcacg cgaagaacca cacttacctg attattatgg agacgaccat ttatggattg
     1081 ttcaaacagt atctcaatat ttaaa
//
LOCUS       DAOTOW010000769         1104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_107649_length_1104_cov_4.303146, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000769 DAOTOW010000000
VERSION     DAOTOW010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107649_length_1104_cov_4.303146"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="P5243_11135"
     CDS             <1..638
                     /locus_tag="P5243_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRS20050.1"
                     /translation="ETNVGAGLPVINTLKDLIKSGDEIIKIEAILSGSLNFIFSNCSS
                     QKDFLSTVKLAREKGYTEPDPKIDLSGKDVARKILILSREIGCTFNLEDVEVENCLSP
                     QSQATTTVEELWKTLEAYDNELYAKKIQEAEAAGKRIKYIASYENGKAKTQITMIDSS
                     HPFYNIMGSDNIISFTTARYRTQPLIVIGPGAGAAVTAAGVFADIIRIGNF"
     gene            647..1033
                     /locus_tag="P5243_11140"
     CDS             647..1033
                     /locus_tag="P5243_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20051.1"
                     /translation="MRIVAVVCAVLIVTSGCKHSQQFVSSVSLASHKWIEHTVWKLSY
                     NHREWGNRTFEVFFGDNGRLVCFNPYDTTPYNDEWELHDDKIILYFNNRDAVYEAVVF
                     DDEVLVGTAHSYIGGTWNWIAQRYKQ"
BASE COUNT          379 a          145 c          230 g          350 t
ORIGIN      
        1 atgaaactaa cgtaggtgca ggtttgccgg taataaatac attaaaagat ttaattaaaa
       61 gcggtgatga aattataaaa attgaagcaa ttttatcggg ctcacttaat tttatattta
      121 gtaattgttc atcacaaaaa gactttttgt caactgtgaa attggctcgc gaaaaaggat
      181 atacagaacc cgatcctaaa attgatttgt cgggtaaaga tgtagctcgt aaaattttga
      241 tattgtctcg tgaaatcgga tgtacattta atttagaaga tgttgaggtt gaaaattgtt
      301 taagccctca aagtcaagca actacaacag tagaagaatt gtggaaaaca ttagaagcat
      361 atgataatga attgtatgca aaaaaaatac aagaggcaga ggctgcaggt aaacgtatta
      421 aatatatagc aagttatgaa aatggtaaag caaaaacgca aattaccatg atagattcat
      481 cacatccttt ttataatatt atgggaagcg acaatataat ttcgtttact actgctcgtt
      541 acagaacaca acctcttata gtaatagggc ccggtgccgg tgctgctgta actgctgcgg
      601 gagtatttgc agatattata cgaataggta acttttaatg tgctatatgc ggattgttgc
      661 agtagtttgt gcagtattga ttgtaacaag cggatgtaaa cattcgcaac aatttgtgtc
      721 ttctgtgtct ttggcatcgc acaagtggat tgaacacaca gtgtggaaat tatcatataa
      781 tcatcgagaa tggggaaatc gtacgtttga agtatttttt ggtgacaatg gtaggttagt
      841 atgttttaat ccgtatgata ctactccata taatgatgaa tgggaattac atgacgataa
      901 aataatcttg tactttaaca atcgcgatgc tgtgtatgaa gctgtagtgt ttgatgatga
      961 ggttttagtt ggtactgctc acagctatat tggaggcacg tggaattgga tagcacagcg
     1021 atataaacaa taattgatta taaagattat aggttatttg taataaaaaa aggtgtttga
     1081 ttaagcaatc aaacaccttt tttt
//
LOCUS       DAOTOW010000770         1102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_108004_length_1102_cov_3.723973, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000770 DAOTOW010000000
VERSION     DAOTOW010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108004_length_1102_cov_3.723973"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="P5243_11145"
     CDS             complement(<1..571)
                     /locus_tag="P5243_11145"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRS20052.1"
                     /translation="MKIVVTTLLSIVLYTSTLFAYSYIQVVDPRNGGLWTQGTIKKAT
                     VIISPQGDYALYDLELELGVGKLSNSKSYDSLEITCNFSLPAGSYVTAASLLIGTEWV
                     QANVLPRPEAHAIYEGFVKRRVDPLIVYKNYDDTYVLKIFPISPTNTRTMRLSYAMPL
                     LSTNGFQSADVVLDLIKASQEPVDVSVLIK"
     gene            complement(649..1038)
                     /locus_tag="P5243_11150"
     CDS             complement(649..1038)
                     /locus_tag="P5243_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20053.1"
                     /translation="MKYILYLCAFLVIALVSSCKKQTKTVIANIVWNDSHFVYVDDTW
                     YAAIAEGTSDNYYVLSYKQLTRVLQGVNKKISFSVEDSKAAKHTIVIFNDSNGDKKFT
                     EADTYFSVQTVALSTEKEITVDISVSY"
BASE COUNT          392 a          193 c          158 g          359 t
ORIGIN      
        1 ttttgattaa aacactaaca tcaaccggtt cttgcgatgc tttaattaaa tctaacacta
       61 catctgctga ttgaaatcca ttggttgaca ataatggcat agcataactc aaacgcatag
      121 ttcgagtatt tgtaggtgaa ataggaaaaa ttttcaacac atatgtatca tcgtaatttt
      181 tgtatacaat gagcggatct actcgtcgtt ttacaaatcc ctcgtaaatg gcatgagcct
      241 ccgggcgagg taaaacattt gcttgcaccc attcagttcc aattagcaaa gaagctgctg
      301 ttacataaga cccggcgggt agcgaaaaat tacaagttat ttcaagtgaa tcatatgatt
      361 ttgaattaga taattttcca acacctaatt ccaattccaa atcatataac gcataatctc
      421 cctgtggaga aattattaca gtagcttttt taatagttcc ttgcgtccaa agcccgccat
      481 ttcgagggtc tacaacttgt atataagaat atgcaaataa tgtagaagta tataaaacta
      541 tacttagaag agttgtaact acaattttca tagaagtaat ttttggttat ttatatagac
      601 aaataaacgc acaaaatccc tataaaaaaa ataatatatt ctaacctatt aatatgagac
      661 agatatatca acggtaattt ctttttctgt actcagagca acagtttgaa cagaaaaata
      721 tgtatcggct tcagtaaatt ttttatcacc attactatcg ttaaaaataa caattgtatg
      781 tttagccgct tttgaatctt ctaccgaaaa cgaaatcttt ttattaactc cttgcaaaac
      841 tcttgtaagt tgtttatatg acaacacata gtaattatca gacgtacctt cggcaatagc
      901 agcataccac gtatcatcta catatacaaa atgactgtca ttccacacaa tattggcaat
      961 aactgtttta gtttgttttt tacaagaaga aactaatgct attacaagaa atgcacacag
     1021 atataaaata tacttcatac tatatttatt atcactgttg tccgataaaa aattaagaga
     1081 aggaagatgt tagtcttcct tt
//
LOCUS       DAOTOW010000771         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_108366_length_1100_cov_2.452632, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000771 DAOTOW010000000
VERSION     DAOTOW010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108366_length_1100_cov_2.452632"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1100)
                     /locus_tag="P5243_11155"
     CDS             complement(<1..>1100)
                     /locus_tag="P5243_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRS20054.1"
                     /translation="HLDEPKERIQQLFEKAVAPKHSIFDALKMLPLLHELQKWMPKYK
                     KGKGACQHIIQTNVSLDSLPILQCWPHDGGKFITLPMVITKHPITGIRNVGMYRMQVI
                     DSTNTAMHWHLHKTGANHYKAYKELGIRMPVSVALGGDPSHTFAATAPLPENVDEFML
                     SGFLRRKHVELVQCITNELYVPADADIVLEGYIDTTEELFFEGPFGDHTGFYSLPDFY
                     PTFHVTCITYKKNAIYPTTIVGIPPMEDAWIGKATERIFLAPIKLLLAPELIDYWMPF
                     EGVAHNLVIAKIQKQYAGQAYKVMNTLWGAGQMMFNKTLIIIDENTEMNIDAICNAIF
                     AYCNVQHDIYLSKGPLDVLDHAAPQTGFGSKI"
BASE COUNT          343 a          191 c          186 g          380 t
ORIGIN      
        1 tattttactg ccaaatcctg tttgtggagc tgcatggtct aatacatcaa gaggcccttt
       61 acttaaataa atatcatgct gtacgttaca atatgcaaat attgcattgc aaatagcatc
      121 aatgttcatt tctgtatttt catcaattat tataagtgtt ttattaaaca tcatttgccc
      181 agctccccat aacgtattca ttactttgta tgcttgacct gcatattgtt tttgtatttt
      241 ggcaataacc aaattatgag caacaccttc aaaaggcatc caataatcaa taagttcggg
      301 tgctaataat aattttattg gtgcaagaaa tatgcgttcc gttgcttttc ctatccacgc
      361 atcttccata ggaggtatac caactatagt ggtaggatat attgcatttt ttttatacgt
      421 aatacatgtt acatgaaatg ttggatagaa gtcgggaagc gaataaaaac ctgtatgatc
      481 accaaatggt ccttcaaaaa ataattcttc agtagtgtct atatatcctt ctaaaacaat
      541 gtcagcatca gcgggaacat ataattcatt ggtaatacat tgtactaatt ctacatgttt
      601 tctacgtaaa aaacctgata acataaattc gtcaacattt tcgggcagag gtgctgtggc
      661 tgcaaatgta tgagaagggt caccacctaa tgctacagat accggcatac gaatacctaa
      721 ttctttatat gctttgtaat gattagctcc ggttttgtgt aaatgccaat gcatggctgt
      781 gtttgttgaa tcaattactt gcatacgata cataccaaca ttacgaatac ctgttatagg
      841 atgtttggta attaccattg gtaatgtaat gaatttgccg ccatcatgag gccaacattg
      901 taatattggt aatgaatcta aagaaacatt tgtctgaata atgtgctggc atgcaccttt
      961 acctttttta tatttgggca tccatttttg taattcatgt aataatggca acattttgag
     1021 agcatcaaaa atagaatgct taggagcaac agctttttca aacaattgtt gaattctttc
     1081 tttcggttca tcaagatgag
//
LOCUS       DAOTOW010000772         1098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_108764_length_1098_cov_1.634708, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000772 DAOTOW010000000
VERSION     DAOTOW010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108764_length_1098_cov_1.634708"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="P5243_11160"
     CDS             <1..278
                     /locus_tag="P5243_11160"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRS20055.1"
                     /translation="DGLFKVTKADRFGFVDTTNTVIVPLEYDWCFNFFEHVAIVKKNN
                     LYGVVHDTGTLVADCVYSCIKPFAQGKAIACKDSVWGVLHIDGSFSV"
     gene            292..825
                     /locus_tag="P5243_11165"
     CDS             292..825
                     /locus_tag="P5243_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase domain-containing protein"
                     /protein_id="HRS20056.1"
                     /translation="MLIVYNQISICCISDTHGKHRDLSMPAADILIHCGDACTDGNIK
                     QLQDFFAWFSELEYRHKIFVPGNHDLLFELHPIEFYAMMPNQILLLHDSHIEIMGIRF
                     LALSPYFTYTYHGKTKVDILLSHQPAYGVLDAHVGSVDLRNFIENYSPSYCICGHIHD
                     KRGMLQVANTTYVNCCI"
     gene            complement(830..>1098)
                     /locus_tag="P5243_11170"
     CDS             complement(830..>1098)
                     /locus_tag="P5243_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20057.1"
                     /translation="SNFESACSNIQNYTYVLCFTTVSDEYLSIIESHNNSSDYESIIL
                     GNDLVKLLPRPKRQGFSNQFINKQRVVDSSFHEHIRLTCFFIQT"
BASE COUNT          332 a          179 c          211 g          376 t
ORIGIN      
        1 gtgacggttt gttcaaagta accaaagcag atagatttgg ttttgtagac actacaaata
       61 cggtaattgt tccccttgaa tatgattggt gcttcaactt ttttgagcat gtggcaattg
      121 ttaaaaagaa taacctatat ggtgtagtgc atgatacggg aactttagta gccgattgcg
      181 tgtattcgtg cattaagcct tttgctcaag gcaaagccat tgcatgtaaa gattctgttt
      241 ggggtgtttt acatatcgat ggttcatttt cggtataaat aacaatttat aatgcttatt
      301 gtatacaatc aaatttcaat ttgctgcatt tcagatacgc atggcaagca ccgagattta
      361 tcgatgcctg ccgccgatat tcttatacac tgtggcgatg cgtgcacaga tgggaatata
      421 aagcaattac aagatttttt tgcatggttt tccgaattag agtaccggca caaaatattt
      481 gtacctggca atcacgattt gctttttgaa ttacacccca ttgagttcta tgccatgatg
      541 cccaatcaaa ttcttttgtt acacgattcg catatagaaa ttatggggat tcgttttctt
      601 gcattatcac catattttac ctatacatat catggaaaaa ctaaggttga tattcttttg
      661 tcgcatcaac cggcttatgg agtattagat gcacatgttg gtagtgtaga tttgagaaac
      721 tttatagaaa attattctcc atcgtattgt atctgtgggc atatccatga taaacgcggt
      781 atgctgcaag ttgcaaatac aacatatgtg aactgttgta tataacgcct taggtttgta
      841 taaaaaaaca agtaagtcgt atatgttcgt gaaacgaact atcaaccaca cgttgtttgt
      901 taataaactg attcgaaaac ccctgtcgtt tcggtcgtgg taataatttc actaaatcat
      961 tacctaatat aattgattca taatctgaag aattattgtg tgattctata atcgataaat
     1021 actcgtcgga gacggtggta aaacagagta catatgtata attttgtatg tttgagcatg
     1081 ctgattcaaa atttgaac
//
LOCUS       DAOTOW010000773         1097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_108863_length_1097_cov_2.285988, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000773 DAOTOW010000000
VERSION     DAOTOW010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108863_length_1097_cov_2.285988"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>1097)
                     /locus_tag="P5243_11175"
     CDS             complement(172..>1097)
                     /locus_tag="P5243_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20058.1"
                     /translation="GYERPVKIANIFKGICAGFIAILIVYALFPESLRYSRALIFFTC
                     IWGILLLPIIRVIFHVLKISNFRILIQKNKQIVLVGDEQENTRVYNFLKQTNIQVQIV
                     GFVSIENKVHDTQYLGTLNQLQEIVNIYGINEIIFCMRDVGASNIIKHMLEFSDLQVD
                     FKIAPPESFSVIGSNSSNSTGELYTIELNSVAKGLNKKKKRMFDIVTSLMLILGSIFL
                     CWFVKHKQKFIKHCFEVLLGSKTWVGYAPQLQELQKSLPKLKPGIVYPGMQLHTNNIQ
                     ILEQAHIVYVKDYKMRNDALIVIACFNQLGN"
BASE COUNT          391 a          180 c          122 g          404 t
ORIGIN      
        1 gccaatagta ttaatagttt gttttgaaat aagattgacc cctaatccaa caaaacttaa
       61 cccaacatta cctgaaaatg tagatttata atacattttt tgttgcgaaa acgcaaatga
      121 tgtcattaca atacatccta ctatgaaagc tattcttttt ttcatatttt tttagttacc
      181 taactgatta aaacaagcta ttacaattaa agcatcattt ctcattttat aatctttaac
      241 atatacaata tgagcttgtt ctaaaatttg aatattattt gtatgtaatt gcattcccgg
      301 gtatacaatt ccaggcttta attttggtaa acttttttgt aattcttgta attgaggtgc
      361 ataccccacc cacgtttttg aacctaataa tacttcaaaa caatgtttta taaacttctg
      421 tttatgtttt acaaaccaac aaagaaatat acttcctagt attaacatca gagatgttac
      481 aatatcaaac attcgttttt tctttttatt taatcccttt gcaacagaat taagttctat
      541 agtatataat tcaccggttg aatttgatga attactgcca ataacagaaa atgattccgg
      601 gggagctatt ttaaaatcaa cctgtaaatc agaaaattct aacatatgct taattatatt
      661 acttgcacct acatcgcgca tacaaaatat aatttcgttt attccatata tatttacaat
      721 ttcttgtaac tgatttagcg tacctaaata ttgtgtatca tgcactttat tttcaatact
      781 tacaaatcct actatttgaa cttgtatatt tgtttgtttc aaaaaattat aaactcgagt
      841 attttcttgc tcatcaccta ccaaaactat ttgtttattc ttttgaatta aaattctaaa
      901 attactaatt ttaagaacat gaaaaataac acgtatgata ggcaataata atattcccca
      961 aatacaagta aagaaaataa gtgctcgtga ataccgtaaa gactcaggga ataatgcata
     1021 tactataagt atagcaataa agcctgcaca tattccttta aaaatattag caatttttac
     1081 gggacgttca taacctg
//
LOCUS       DAOTOW010000774         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_109739_length_1091_cov_3.088803, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000774 DAOTOW010000000
VERSION     DAOTOW010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109739_length_1091_cov_3.088803"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="P5243_11180"
     CDS             complement(<1..689)
                     /locus_tag="P5243_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20059.1"
                     /translation="MSFTHEVEGMICVAKGANHGPAPIPQVGKWVQAKEVKDISGLTH
                     GVGWCAPQQGACKLTLNVKDGIIEEALVETIGCTGMTHSAAMAAEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQTAFSEGGLPVGAGLEDLGKGLRGVTGTLYGTSAK
                     GPRYLEMAEGYITEIGLDEQNQIIGYRFVNLGRMMDMIKAGTDANEAIKKASGKYGRI
                     EDAVKVIDPRK"
BASE COUNT          319 a          211 c          151 g          410 t
ORIGIN      
        1 tctttacgtg ggtcaataac tttaactgca tcttctattc gaccgtattt tcctgatgct
       61 tttttgattg cttcatttgc atcggttccg gcttttatca tatccatcat acgacctaaa
      121 tttacaaaac gatatccaat gatttgattt tgttcatcaa gaccaatttc tgtaatatac
      181 ccttctgcca tttcaagata acgaggacct tttgctgatg ttccgtataa cgtgccggtt
      241 acaccacgta agcctttacc taaatcttca agtccggcac ctacaggtaa tccaccttca
      301 gaaaatgcag tttgtgtacg accgtatact atttgcagga acaattcacg cattgctgta
      361 tttatagcat cacacaccaa gtcggtattc aatgcttcta aaattgtttt tcctggtaaa
      421 atttcggcag ccattgcagc tgagtgagtc ataccggtac aaccaatagt ttcaactaaa
      481 gcctcttcaa ttattccatc tttaacattt agtgtcaact tacatgcacc ttgttgtgga
      541 gcacaccatc ctacaccatg ggtcaatccc gaaatgtctt ttacttcttt tgcttgtacc
      601 catttgccta cctgaggtat gggtgcagga ccatgatttg caccttttgc aacgcaaatc
      661 attccttcta cttcgtgtgt aaaactcata tttctattaa tttatttgtt tttttaaaat
      721 tttgccacaa atataaaaag tttatttata atgttatcac aaacattttt tttagataac
      781 aatctgcatc attttttttt atgagaataa tatattttat tttttttcac gcttaacaca
      841 taaaaaaagc acagctctat tttatagaaa ctatgcttta actgaaaaac agggttttat
      901 acgtatctat taaacaaaag tagcgtaatc tacattttaa ttttgtgagt tgctataagt
      961 aattgttgtt tataaattga caatatatat ataccttctt gtaaactata aggacatata
     1021 atagtttctg taggtgcttt acaagtgtaa ctatatttta tttttcctga ttctttaatt
     1081 acagtaatag t
//
LOCUS       DAOTOW010000775         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_110051_length_1089_cov_3.151838, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000775 DAOTOW010000000
VERSION     DAOTOW010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110051_length_1089_cov_3.151838"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="P5243_11185"
     CDS             <1..266
                     /locus_tag="P5243_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS20060.1"
                     /translation="NNIKGQTSLQEISHTTQYVYPNPCKSHIGISIDDAYMISIYTLL
                     GQELMQIEVSDSTNIDISTLPAGMYIVQINTLNQVLYQLITKQ"
     gene            complement(268..471)
                     /locus_tag="P5243_11190"
     CDS             complement(268..471)
                     /locus_tag="P5243_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS20061.1"
                     /translation="MRFNFVPQSTISIEIRDMQGKIIKIMQVPTQKNKQEITVNFTKI
                     PAGTYILHISDIEHKSSVNFIKK"
     gene            complement(697..773)
                     /locus_tag="P5243_11195"
     tRNA            complement(697..773)
                     /locus_tag="P5243_11195"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(737..739),aa:His,seq:gtg)
     gene            896..>1089
                     /locus_tag="P5243_11200"
     CDS             896..>1089
                     /locus_tag="P5243_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20062.1"
                     /translation="MPRFIADFHIHSKYSMATSKQLTPKWLDYWARIKGISVVGTGDF
                     THQKWTAELYEQLQPAEQGL"
BASE COUNT          380 a          158 c          170 g          381 t
ORIGIN      
        1 caaacaatat aaagggtcag acatcattac aagaaatttc acatacaact caatatgtgt
       61 atcctaatcc atgtaaatca catataggaa tttcgataga tgatgcttat atgattagta
      121 tatatacatt gcttggacaa gaattaatgc aaatagaagt ttcagattct acaaatattg
      181 atatttcaac tttaccggca ggaatgtata tagtgcaaat aaatacattg aatcaagtgc
      241 tttatcagtt gataacaaag cagtaaatta tttttttata aaatttacag atgatttatg
      301 ttcaatatct gatatatgta atatataggt accggcagga atttttgtaa aattcacggt
      361 aatttcttgt ttgttttttt gtgttggtac ttgcatgatt tttattattt tgccttgcat
      421 gtcacgaatt tcaatactaa ttgttgattg tggaacaaaa ttgaatcgga ttactgcatc
      481 agaaataaca gtgtaagctt cccattttta ggacagtgtt taattataat gtaacaaaga
      541 tattttttat tctcaaataa ttgatgttct aaacaataca aaagaaaaaa ttaatttttt
      601 tatacgtttt tttagagaaa aagtttcaaa taaaattgtg atattaaaag aaaaaattat
      661 gtaataaaaa aggctgccat atttggcagc cttttttggg gtggaagacc gggctcgaac
      721 cggcgaccct tagaaccaca atctaatgct ctaaccaact gagctacaac caccatgtag
      781 aattttggac tgcaaaaata atactttttt atttaccaca aaatattatg ttcaattttt
      841 aacaaattat cactactttt gaaatgctat aatttctaat atatttttta ttgctatgcc
      901 tcgatttatt gccgattttc atattcattc caaatattca atggctacca gtaagcaact
      961 tacaccaaaa tggttagatt attgggctcg cattaagggt atatcggtgg taggcacagg
     1021 cgattttacg catcaaaagt ggactgcaga attgtacgag caattgcaac ctgccgaaca
     1081 aggtttgtt
//
LOCUS       DAOTOW010000776         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_111420_length_1081_cov_2.167641, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000776 DAOTOW010000000
VERSION     DAOTOW010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111420_length_1081_cov_2.167641"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="purH"
                     /locus_tag="P5243_11205"
     CDS             complement(<1..1055)
                     /gene="purH"
                     /locus_tag="P5243_11205"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRS20063.1"
                     /translation="MKDLNVTFYSTGGTQEFLESLGCKVIPVEDVTSYPSILGGRVKT
                     LHPKIFGGILARRELASDIAQMQEYGIPEIDLVIVDLYPFEQTVASGASEQDIIEKID
                     IGGISLIRAAAKNHKDVVIVASQKQYALLDSVLESGKGITTIEQRKEFAREAFNTSSH
                     YDSAIFNYFNNGIDNSEKISLTNGSVLRYGENPHQKAIFYSFSNTKTNSTLANSQVLQ
                     GKELSYNNMLDADAAWKAASDAYHAVAHIPNKTAVSVVKHLNPCGLAVSDSSIKALEL
                     AWAGDPISAFGGILCFTSQVDADVATWLSDKFIEIIIAPSYTDAAKELFAKKKNLRVL
                     VTPIKPEITGEKLYRSI"
BASE COUNT          342 a          200 c          157 g          382 t
ORIGIN      
        1 ttgattgacc gatacaattt ttctccggtt atttcaggtt ttataggtgt taccaataca
       61 cgtaaatttt ttttctttgc aaataactct tttgctgcat ctgtgtatga aggtgctata
      121 ataatttcaa taaatttatc gctcaaccat gttgcaacat ctgcatctac ttgagaggta
      181 aaacacaaaa tacctccaaa cgcactaata gggtctccag cccaagctaa ttctaatgct
      241 ttaattgaac tatcagaaac agctaaacca caaggattta aatgtttcac aactgataca
      301 gctgttttat ttggtatatg agccaccgca tgatacgcat cacttgcagc cttccatgca
      361 gcatcagcat cgagcatgtt attgtaagac aattctttac cttgtaatac ttgagaattt
      421 gctaacgttg aatttgtttt agtatttgaa aatgaataaa atatagcttt ttgatgtgga
      481 ttttcaccat atcgcaacac gcttccatta gttaaactta ttttttcgga attatcaata
      541 ccgttattaa aataattaaa aattgcagaa tcataatgcg atgatgtgtt aaatgcctct
      601 ctagcaaatt ctttacgttg ttcaattgta gtaatacctt tacccgattc taaaactgaa
      661 tctaataacg catattgttt ttgtgatgct acaattacca catctttatg atttttggcg
      721 gcagcacgaa taagagatat acctccaata tcaatttttt ctattatatc ttgttctgaa
      781 gcacccgaag ctactgtttg ttcaaacgga tataaatcta ctattactaa atctatttcg
      841 ggtattccat attcttgcat ttgtgcaatg tcacttgcaa gttctctgcg agctaatata
      901 cctccaaata ttttgggatg cagagttttt actcgtccac ctaaaattga gggatatgaa
      961 gtaacatctt caaccggaat aactttacaa ccaagtgatt caagaaattc ttgtgtacca
     1021 cctgtagaat agaaagtaac gtttaaatct ttcaatttat aaactatact atctaaatta
     1081 t
//
LOCUS       DAOTOW010000777         1080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_111539_length_1080_cov_3.484878, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000777 DAOTOW010000000
VERSION     DAOTOW010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111539_length_1080_cov_3.484878"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="P5243_11210"
     CDS             complement(<1..387)
                     /locus_tag="P5243_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS20064.1"
                     /translation="MKIFENILKKYWGYTKFRPLQLEIIRSVHSGVDTLGLMPTGGGK
                     SITFQIAGLAKEGICIVVTPLIALMKDQVDSLRQKGIKAIAIYSGMNYKEIEIAYDNC
                     TYGNVKFLYISPERLKTDMFRERIRNM"
     gene            complement(491..>1080)
                     /locus_tag="P5243_11215"
     CDS             complement(491..>1080)
                     /locus_tag="P5243_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRS20065.1"
                     /translation="EVGSFMGLAAMTGSEITIKNVSYNNLGIIPQSFARLGIQFEKRN
                     DDIYIPSQNSYTIPTFIDGSIMHLADAPWPGLTPDLLSVLLVVATQASGSVLIHQKMF
                     ESRLFFVDKLIDMGCQIILCDPHRATVIGLGNKQKLRATTMSSPDIRAGVALLIAAMS
                     ADGTSTIHNIEQIDRGYERIDERLNAIGAEIIRIQ"
BASE COUNT          344 a          183 c          168 g          385 t
ORIGIN      
        1 catatttcga attcgttctc gaaacatatc tgttttgagt cgttccgggg atatatataa
       61 gaattttaca tttccatatg tacaattatc atacgctatt tcaatttctt tgtagttcat
      121 tcccgagtat atggcaatag cttttattcc tttttgacgc aatgagtcta cttggtcttt
      181 cataagagca ataaggggag ttacaactat acatatacct tcttttgcta aacctgcaat
      241 ttgaaacgtt attgattttc ctccaccggt aggcataagt ccaagtgtat ctactcccga
      301 atgtacactt ctaattattt caagttgaag aggtctaaat ttggtatatc cccaatattt
      361 ctttaatatg ttttcaaaaa ttttcaattg ctactttgat aagtttttaa ggtatagtat
      421 tgtataatac tataaaaata gtaaaaaata ctatattact tatataatgg aataaaaaag
      481 tgaaaattgc ttattgaatt ctaataattt ctgcacctat agcattgagg cgttcatcaa
      541 tacgttcgta gccacgatca atttgttcaa tattgtgaat agtactagtt ccatcggcag
      601 acattgctgc tattaaaaga gcaactcctg ctctaatatc tggagaagac atggttgttg
      661 cacgtaattt ttgtttattt cctaatccaa taactgttgc tcggtgtggg tcacataata
      721 ttatttggca gcccatatct atgagtttat caacaaaaaa caatctgctt tcaaacattt
      781 tttgatgtat caaaacacta cctgatgctt gtgttgcaac aactaataaa acacttaata
      841 agtcaggagt taagccgggc caaggagcat ctgcaaggtg cattatagaa ccatcaataa
      901 atgtaggaat tgtataggag ttttgtgaag gaatataaat atcatcattt cttttttcga
      961 attgaattcc taatcgggca aacgattgcg gaataattcc taaattatta tatgatacgt
     1021 ttttaatagt tatttctgaa ccggtcattg ctgctaatcc cataaaacta ccaacctcta
//
LOCUS       DAOTOW010000778         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_111930_length_1078_cov_2.421310, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000778 DAOTOW010000000
VERSION     DAOTOW010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111930_length_1078_cov_2.421310"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="P5243_11220"
     CDS             complement(<1..999)
                     /locus_tag="P5243_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRS20066.1"
                     /translation="MNTPLPFLHICIQDILIKHGTDFSNSIIILPNKRPILFIKKYLS
                     QEITTPCFLPKFYTIQEFFMLHTQRSIVHEIQCIHIIYTLYKKYTGSTETFEEFYSWG
                     EMLLQDFNDIDTYCIPVEDLFANLSAIKEFTNTFDYLSEEQIAIITRFWDTTKHTTQQ
                     SIQTKFLTIWQALLNIYKDCKQELSTQKLGYKGMIFREIVENASSMIQLSDSTKLFFI
                     GFNALNTCEIQLFTFCKTKKQAYFYWDYDQYYATKTHEAGYFITKNIKLFGNEIHQDY
                     YNAFKKPKQINIIETPNPISQVKQCESLLKSYTSNSGAIVLADEQLLIPLLQSIPET"
BASE COUNT          338 a          115 c          189 g          436 t
ORIGIN      
        1 agtttcggga attgattgta gtaaaggtat taataattgt tcgtcggcaa gtactattgc
       61 acccgaattt gatgtatacg atttgagtaa tgattcacat tgtttgacct gagaaattgg
      121 atttggagtt tcaattatat taatttgttt tggtttttta aaagcattgt aataatcttg
      181 atgaatttca ttcccaaata gtttaatgtt ttttgttata aagtaacctg cctcatgagt
      241 ttttgttgca taatattggt cgtaatccca ataaaaataa gcttgttttt ttgttttgca
      301 aaatgtaaaa agttgaattt cacatgtatt cagggcattg aatccaataa aaaataattt
      361 tgttgaatcg cttagttgaa tcatagacga agcattttca acaatttctc tgaaaatcat
      421 gcctttgtat ccaagttttt gtgtgctaag ttcttgtttg caatctttgt atatattcaa
      481 tagtgcttgc caaatagtaa ggaattttgt ttgaatagat tgttgggttg tatgttttgt
      541 ggtatcccaa aatcgagtga taatagcaat ttgttcttct gataaataat caaatgtatt
      601 agtaaattct ttgatagctg atagatttgc aaacaagtct tctacaggaa tacaatatgt
      661 atcaatatca ttaaaatctt gcagaagcat ttctccccat gaataaaatt cttcgaacgt
      721 ttcggtgctt cctgtgtatt ttttgtatag tgtgtaaatt atatgaatac attgtatttc
      781 atgtacaata cttcgttgag tatgtaacat aaagaattct tgaattgtat aaaattttgg
      841 taaaaaacaa ggggttgtaa tttcttgtga gaggtatttt tttataaaaa gtataggtcg
      901 tttgttaggt aatataataa ttgaattgga aaaatcggtt ccatgtttta tgagaatatc
      961 ttgtatgcaa atgtgtaaaa atggtagggg ggtattcata ttatacttaa gttttgtttt
     1021 tcaaatatac catttttttg aaaagggatt aaatacaatg tgtgttattt tttatttt
//
LOCUS       DAOTOW010000779         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_112090_length_1077_cov_2.519569, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000779 DAOTOW010000000
VERSION     DAOTOW010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112090_length_1077_cov_2.519569"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /locus_tag="P5243_11225"
     CDS             <1..>1077
                     /locus_tag="P5243_11225"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS20067.1"
                     /translation="PVTFTDLSTSNNPITNWDWQIQREDGTITLTSTVKNPVITLTDT
                     GSYNVRLTVTDNAGCTNTLTRNDFVGAGMTPTSDFQTILTTVCFNTPMDFEDLAESWC
                     NNWIWDFGDGTTQTGKNPTYVYADTGTYTVSLRAEHYHCPNIAVKTDYVTILPPIARY
                     TINPTVLCAFPDTIQFTDASVLADEYYWDFGDGMKLSIIGNTWTWSNGVDPDKIETIV
                     GNKSPKYEYTAAGNYTSKLRVVNTTYSCEDSIEVAFVASDVVPQFTQNKNQSCQHTGV
                     SFTDISTTDIGSISAWEWDYGDGTPYGTTATPTHIYTTSGNFDVRLVAFDSNGCSDTI
                     TKVANVVIYQVPSVQFTANKITGCA"
BASE COUNT          354 a          188 c          207 g          328 t
ORIGIN      
        1 accggtgaca tttactgatt taagtacgtc taataatcct ataacgaatt gggattggca
       61 aattcaacga gaagatggta caattactct tacctctact gttaaaaatc ctgtaataac
      121 attaactgat acaggttcat ataatgttcg gctaacagta accgacaatg cagggtgtac
      181 aaatacgcta actcgcaatg attttgttgg agcaggaatg acaccaacat ccgattttca
      241 aacaattcta acaaccgtgt gttttaatac accaatggat tttgaagatt tagccgagtc
      301 atggtgtaac aactggattt gggattttgg tgatggcact acgcaaaccg gtaaaaatcc
      361 aacatatgta tatgctgata cgggaacata tacagtatct ttgagggcag aacattatca
      421 ttgtccaaat attgccgtaa aaacagatta tgtaactatt ttaccaccta ttgcacgtta
      481 taccattaat cctacggtgc tttgtgcttt tcctgataca attcaattta ctgatgcatc
      541 ggtacttgcc gatgaatatt attgggattt tggcgatgga atgaaacttt ctataatagg
      601 gaacacatgg acatggagta atggtgttga tccagacaaa attgaaacaa ttgttgggaa
      661 taaaagtcca aaatatgaat acacagcagc aggaaattat acaagtaaat tacgagtggt
      721 aaatacaact tattcgtgcg aagattctat tgaagtggca tttgttgcat ctgatgtagt
      781 accacaattt actcaaaaca aaaatcaaag ttgccaacac accggtgtta gttttaccga
      841 tatttctaca acagatattg ggtcaataag tgcatgggag tgggattatg gtgatggaac
      901 accttatggt actacagcaa cacctacaca tatttatact acatcaggaa attttgatgt
      961 tcgtcttgtt gcttttgatt ccaacggttg tagcgatacc ataaccaaag ttgctaatgt
     1021 tgttatttat caggtaccat cggtgcaatt tactgccaat aaaattaccg gatgtgc
//
LOCUS       DAOTOW010000780         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_112268_length_1076_cov_2.899119, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000780 DAOTOW010000000
VERSION     DAOTOW010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112268_length_1076_cov_2.899119"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="P5243_11230"
     CDS             <1..777
                     /locus_tag="P5243_11230"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRS20068.1"
                     /translation="VWTPESVIGSNRILAEAAEKIPAMGWGPREAARVYNAMIDPITR
                     FKIAGVLWYQGETNVANADTYHVLFPELIKSWRQAWGYNFPFLFAQIAPFTYNETPTC
                     GADIRNAQRQTLSLPNTGMVVTADITADTMNIHPTNKQDVGKRFAHIALNKVYNKPQF
                     PVSGPLYKSHTVKGNVVTIQFDYAQGLHAKSKITGFEIAGENMMFKPADAKIKGTTIE
                     ISSKQVAKPVYVRYAYRNASLIELFNSQGLPASTFQFRIW"
     gene            795..>1076
                     /locus_tag="P5243_11235"
     CDS             795..>1076
                     /locus_tag="P5243_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRS20069.1"
                     /translation="MNTSVDSIKVRVQKRIIAISILLFVAKIIVFLITNSVGILTDAL
                     ESIVNIVTAIISLYSIRVALKPYDDDHPFGHGKVEMLSASLEGILIILAG"
BASE COUNT          345 a          180 c          207 g          344 t
ORIGIN      
        1 gtgtggactc ccgaatcggt aataggttca aatagaattt tagcagaagc tgcagaaaaa
       61 attcctgcta tggggtgggg accacgcgag gcagctcgtg tatataatgc catgattgat
      121 cctattacac gtttcaaaat tgccggtgta ttgtggtatc aaggcgaaac taatgtggct
      181 aatgccgata cgtatcatgt gttgtttccg gaacttatta agtcgtggcg tcaggcgtgg
      241 ggatataatt tccctttctt gtttgctcaa atagcgccat ttacttataa tgaaactcct
      301 acatgcggag ctgatattcg taatgctcaa cgccaaactc tatcgttacc aaataccgga
      361 atggttgtta ctgccgatat cactgccgat actatgaata ttcatcctac aaataaacaa
      421 gatgtgggta aacgatttgc tcatatagct ctcaataaag tgtataataa accacaattc
      481 ccggtttcag gtccgttgta taaatcgcat acagtaaaag gaaatgtagt aactatacaa
      541 tttgactatg ctcaaggatt gcatgcaaaa tcaaaaatta caggttttga aattgccggt
      601 gaaaatatga tgtttaaacc ggctgatgct aaaattaaag gaacaactat tgaaattagt
      661 tcaaaacaag ttgcaaagcc tgtatatgta agatacgcct atcgtaatgc ttctcttata
      721 gaattgttta attcgcaagg attaccggct tctacatttc aatttagaat ttggtaatta
      781 aactatctca tataatgaat acgtctgtag atagtataaa agttagggtt caaaaacgaa
      841 tcattgcaat atcaatatta ttgtttgttg caaaaattat agtgttttta ataactaatt
      901 ctgttggaat actaaccgat gctctcgaaa gtattgtaaa tatagtaact gcaattataa
      961 gtttatatag tattcgtgtt gctcttaaac catacgatga tgatcatcct tttgggcatg
     1021 gtaaagtaga aatgctctcg gcttcgcttg aaggaattct tataattctt gcaggt
//
LOCUS       DAOTOW010000781         1075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_112441_length_1075_cov_2.896078, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000781 DAOTOW010000000
VERSION     DAOTOW010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112441_length_1075_cov_2.896078"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1075
                     /locus_tag="P5243_11240"
     CDS             <1..>1075
                     /locus_tag="P5243_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRS20070.1"
                     /translation="ISWTNAAAGTYSITAKAYDNSGASTTSAARSITVTQPANNTLPS
                     AVITQPHVNAYFQQNTNITIRVYSTDIGGTYTNGTVSKVELYYAPTATPQNTTKLTTL
                     TSHTQNTYTYVWENVPAGSYRITAKATDNLGATFTSAGVTITVGTAAVQAKGMAACKG
                     KYLANIIASSVRSDFGTYWNGVTAENGCKWGSVESTRNTMNWSQADISYNYANTNNLM
                     FRYHAFAWGSQYPSWITGLNTTDFKAEMEQYIAAVAARYPNIDQIDVLNENMYINTYN
                     GQEHAAGTPYFRAGLGGPGVTGYDWVIWLFEKARQYFPNAKLVMNDYELENNTAGINE
                     MLAVVKVLRDRGLIDGFGTQAHCF"
BASE COUNT          394 a          204 c          205 g          272 t
ORIGIN      
        1 aatttcatgg acaaatgctg ccgcaggaac atacagtatt actgcaaaag catatgacaa
       61 ctcaggtgct agtaccacat cggcagcacg cagcataaca gtaacacaac cagcaaacaa
      121 cacattacct tcggctgtta ttacacaacc tcatgtaaat gcatatttcc aacaaaatac
      181 aaatattacc atacgagtat actcaacaga tattggagga acatatacaa atggcactgt
      241 aagtaaagtt gaactgtatt atgccccaac agcaacacct caaaatacta caaaattaac
      301 aaccttaact tcccatacac aaaacacata tacctatgtt tgggaaaatg taccggcagg
      361 ttcataccgg attacggcaa aagcaaccga taatttaggt gcgacattta catctgcagg
      421 agtaacaatt acagtaggta cagccgcagt tcaagctaaa ggaatggcag catgcaaagg
      481 aaaatattta gctaatatta tagcaagtag tgttcgttcc gattttggaa catattggaa
      541 tggtgtaaca gctgaaaacg gctgtaaatg ggggtctgta gaaagcactc gaaatactat
      601 gaattggagt caagcagata tttcatataa ttatgcaaac acaaacaatc ttatgtttcg
      661 ctatcacgcg tttgcatggg gaagccaata tccaagctgg attaccggtt taaacacaac
      721 tgattttaaa gctgaaatgg aacaatatat agcagcagta gctgctcgtt atcctaatat
      781 tgaccaaata gacgttctca atgaaaatat gtacataaat acatacaacg gacaagaaca
      841 tgctgcagga accccctatt ttagagcagg attaggagga ccgggagtta caggatatga
      901 ctgggtaata tggttatttg aaaaagcacg ccaatatttt ccaaatgcaa aattagtaat
      961 gaatgattat gaattagaaa ataatactgc aggtatcaac gaaatgcttg ctgttgtaaa
     1021 agtgttacgc gatagaggac ttattgacgg atttggaacc caagctcatt gcttt
//
LOCUS       DAOTOW010000782         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_112579_length_1074_cov_3.921492, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000782 DAOTOW010000000
VERSION     DAOTOW010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112579_length_1074_cov_3.921492"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="P5243_11245"
     CDS             complement(<1..267)
                     /locus_tag="P5243_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20071.1"
                     /translation="MKTYAYFVGLACLVFISCKEKVPEHHIVYEGKYYREPKPVQYDS
                     NGDGKIDASDDVIEVLGNSLNVTSAPGGKPLYKTNEIITPPGSAT"
     gene            complement(281..991)
                     /gene="pyrH"
                     /locus_tag="P5243_11250"
     CDS             complement(281..991)
                     /gene="pyrH"
                     /locus_tag="P5243_11250"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRS20072.1"
                     /translation="MLKYKRILLKLSGESLQGKEGHGIDPIILNSYANQIKDVVEIGV
                     EIGVVIGGGNIYRGLKGENQGYDRVKGDYMGMLATVINALALQSALECIGVRSRVMTA
                     IDMEPIGEAYSKLRMEHALTQKEVIIFGAGTGNPFFTTDTASALRATEMGADVLLKGT
                     RVDGIYNADPEKDPKAIKFEEITFTEAYEKKLQVMDLTSFTMCREHNIPIIVFNINKN
                     EDLKKVVMGEKIGTIVKN"
BASE COUNT          334 a          210 c          128 g          402 t
ORIGIN      
        1 tgtagcacta ccgggaggag taataatttc gtttgtttta tataatggtt tacctcctgg
       61 agcactagtt acgtttaaac tatttcccaa tacttcaatt acatcatcag aagcatcaat
      121 tttaccatca ccatttgaat catattgtac aggctttggt tctctataat atttaccttc
      181 atacactata tgatgctccg gaactttctc tttacaagaa ataaacacta aacatgctaa
      241 tcctacaaaa tatgcatatg ttttcatggt ataaagggta ttaattttta acaatagtac
      301 caattttttc tcccatcaca actttcttca aatcttcatt tttattaata ttgaaaacaa
      361 taattggaat attatgttct ctacacattg taaatgacgt taaatccatt acttgtagtt
      421 tcttttcata cgcttcggta aacgtaattt cctcaaattt aattgctttt ggatctttct
      481 caggatctgc attataaatt ccgtctactc ttgtgccttt taataataca tctgctccca
      541 tttctgttgc tcgtaaagcc gatgctgtat ctgttgtaaa aaaaggatta cctgtacctg
      601 ccccaaaaat tataacttct ttttgagtaa gagcatgttc cattcgtagt tttgaatatg
      661 cttctcctat tggttccata tcaatagctg tcataacacg agaacgaacc ccaatacatt
      721 ctaaagcaga ttgtaaagct aaagcattta ttacagtagc taacatgccc atataatcac
      781 ctttcacacg atcgtatcct tgattttcgc cttttaatcc acgataaata ttacctcctc
      841 ctattacaac tcctatttca acacctattt caacaacatc cttaatttga tttgcatatg
      901 agtttagtat tattggatca ataccatgcc cttctttacc ttgaagagat tctccactta
      961 attttaaaag tattcttttg tattttaaca taggttttca attaatgtga taaaattaaa
     1021 aaaaaaaaag gagagttgta tatgataact ctccttttgc aattaatttt taca
//
LOCUS       DAOTOW010000783         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_112667_length_1074_cov_1.926398, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000783 DAOTOW010000000
VERSION     DAOTOW010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112667_length_1074_cov_1.926398"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..>1074)
                     /locus_tag="P5243_11255"
     CDS             complement(16..>1074)
                     /locus_tag="P5243_11255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRS20073.1"
                     /translation="IRTPMNGILGFADLLKDPELSLQERMHYVSIIEQSGHRMLTIIN
                     DLIDISKIESGQMTVSYELVDIYKLCKELYDFFIYETQKKQLELIYTPFTESCIIETD
                     VTKITQILTNLIKNAIKFTHSGSIEFGFTIEKNDIRFFVKDTGIGIHPQLQEYVFDRF
                     RQANLSAYKSEDGIGLGLSICKAFVEMLGGKIWVESEVNVGSNFIFTLPAEILHNIDN
                     KTIEIAEPIIQDGLTILIAEDDIANFILLEKILRTHTNARILHAQNGEDALRIFKEEP
                     TIDIALLDIKMPIIDGIELTSIIKKIKPLFITGNHNIKKVSIAISYKAGKYFTVAIAQ
                     KRINLSIYSTKRNHIRSK"
BASE COUNT          320 a          163 c          169 g          422 t
ORIGIN      
        1 caaactaaca aatgttcatt tgctccttat atgattgcgc ttggtgctgt aaatactaag
       61 gtttattcgt ttttgggcga tagcaactgt gaaatacttc cctgccttat aactgatggc
      121 gatactaact tttttgatgt tgtgatttcc tgtaataaaa agaggtttta tttttttaat
      181 gattgatgtt agttctatac cgtcaattat tggcatttta atgtcgagta gagcaatatc
      241 tattgtaggt tcttctttaa aaattcgtaa ggcatcttca ccgttttgag catgtaaaat
      301 acgtgcatta gtatgagttc gtaatatttt ttccaatagt ataaaattgg ctatatcatc
      361 ttcggcaatt aatatagtaa gtccatcttg tataataggt tctgctattt ctattgtttt
      421 attgtctata ttgtgtaata tttctgcagg taatgtaaat ataaaattag acccgacatt
      481 aacttcagat tcaacccaaa ttttgccgcc taacatttct acaaatgctt tgcagattga
      541 taatcctaat cctataccgt cttctgattt atatgccgaa agattagctt gtctaaaacg
      601 atcaaacacg tattcttgta gttgaggatg aatgccaata ccggtatctt ttacaaaaaa
      661 acgaatatca tttttttcta ttgtaaaacc aaattctata cttccggaat gagtaaattt
      721 aatggcattt ttaataaggt tagtaagtat ttgagtaatt tttgtaacat ctgtttctat
      781 tatgcacgat tctgtaaatg gtgtatatat aagttcgagt tgttttttct gtgtttcgta
      841 aataaaaaaa tcgtataatt ctttacacaa tttatatata tcaaccaatt cgtaagatac
      901 ggtcatttgc cctgattcaa tttttgatat atcaattaaa tcgtttatta tggtaagcat
      961 tctatgaccg ctttgttcta tgattgacac gtaatgcatt cgttcttgta atgatagttc
     1021 ggggtctttg agtaaatctg caaaccccaa tattccattc attggagttc gtat
//
LOCUS       DAOTOW010000784         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_112754_length_1073_cov_3.721022, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000784 DAOTOW010000000
VERSION     DAOTOW010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112754_length_1073_cov_3.721022"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1073)
                     /locus_tag="P5243_11260"
     CDS             complement(<1..>1073)
                     /locus_tag="P5243_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRS20074.1"
                     /translation="KLSGMTGTAETEAKELWDIYKLEVIVAPTNKPIIREDKEDLVYK
                     TRREKYNAVIQHIEELVKQGRPVLVGTTSVEISELLSRMLTRQKIKHNVLNAKLHKKE
                     ADIVAEAGHSGNVTIATNMAGRGTDIKLTPEVKAAGGLAIIGTERHESRRVDRQLRGR
                     AGRQGDPGSSQFFVSLEDDLMRMFGSDRIARIMDRLGLEEGEVIQHSMISKSIERAQK
                     KVEENNFGIRKRLLEYDDVMNSQREVIYTKRRHALHGERIGVDFANMMYDVCMSIVNN
                     SHGNAEYDEFKFELLRVLSIDCPISEKEYLQLSTEDITEKIYEELISMYERRIQTIAK
                     QAYPVIKDVYEKQSGTYKNIVVP"
BASE COUNT          280 a          211 c          179 g          403 t
ORIGIN      
        1 taggaactac tatatttttg tatgtgccgc tttgtttttc atatacatct ttaataacgg
       61 ggtatgcttg tttggctata gtttgaatac gacgttcata cattgaaatg agttcttcgt
      121 aaattttttc tgtaatatct tcggttgata attgcaagta ttctttttcg cttataggac
      181 aatcaattga caacacgcga agtaattcga atttaaattc gtcatattct gcatttccat
      241 gagaattgtt tacaatactc atacaaacat cgtacatcat atttgcaaag tctacaccta
      301 ttcgttctcc gtgtaaggca tgacgacgtt tagtgtatat tacttcgcgt tgcgagttca
      361 ttacatcatc atattccaat aatcgttttc gaattccaaa gttgttttct tcaacttttt
      421 tctgagctcg ttctatagat ttagaaatca tagaatgttg aattacttca ccttcttcaa
      481 gtccaagtct gtccattata cgagcaatac ggtctgaacc aaacatacgc ataaggtcat
      541 cttcgagcga tacaaaaaat tgtgatgatc cggggtctcc ttgtcgtccg gcacgtccgc
      601 gaagctgtct gtcaactcgt cttgattcat gtcgttcggt acctataatt gctaaaccgc
      661 cggcagcttt aacttcgggt gttaatttga tatcggtacc acgacctgcc atatttgttg
      721 ctatagtaac gttaccggaa tgacctgctt ctgcaactat atcagcttct tttttatgta
      781 attttgcgtt aagtacattg tgtttaattt tttgacgagt taacattctc gacaataatt
      841 ctgaaatttc tacagatgta gtaccaacaa gaaccggacg tccttgtttt actaattctt
      901 ctatatgttg aattacggca ttatattttt cgcggcgtgt tttatatact aaatcctctt
      961 tatcttctcg aataattggt ttgttggtag gagcaacaat cacttctaat ttgtatatat
     1021 cccataattc ttttgcttct gtttctgcag taccggtcat acccgatagc ttt
//
LOCUS       DAOTOW010000785         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_112782_length_1073_cov_2.587426, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000785 DAOTOW010000000
VERSION     DAOTOW010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112782_length_1073_cov_2.587426"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="P5243_11265"
     CDS             <1..886
                     /locus_tag="P5243_11265"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRS20075.1"
                     /translation="LTTIQKYSNDYFTKNNMNIEKQSFPKIMIALDTLLAPHYLPTQY
                     ASLSNEQRKQYIQTIVQNSVLFSQEKNSELLNKLFKEQNKKTVTKINLLLNSDPGIVF
                     VKQTLQHISNTLYRDYLVTLHNLDSIQQQYFTCLFEYKGQKLAPDANRTMRVSFGNVL
                     PYKTADAVTYYSQTTSYGIQQKWNSSVKDYRYNARLDSLLQQKQYGSYSNDTLYINFA
                     ATNHTSGGNSGSPVLNAYGNFIGLNFDRNKEGTMSDFYFDEQFCRNISVDVRYILFCI
                     EKYGNAYNILQELEFVNH"
BASE COUNT          401 a          132 c          157 g          383 t
ORIGIN      
        1 acttacaact atacaaaaat acagtaatga ctattttact aaaaacaata tgaatattga
       61 gaaacaatca tttcctaaga ttatgattgc gttagataca ttgttggcac ctcattattt
      121 accaactcaa tatgcttcac tttcaaatga acagcgtaag caatatatac aaacaattgt
      181 acaaaatagt gtgttgtttt ctcaagaaaa aaacagtgag ttactaaata aactttttaa
      241 agagcaaaat aagaaaactg ttaccaaaat aaatttactt cttaattcag atccgggaat
      301 tgtatttgta aaacaaacct tgcagcatat tagcaataca ttatatagag attatttagt
      361 tacattacat aatttagata gtattcaaca acaatatttt acatgtttat ttgaatataa
      421 aggtcaaaaa ttggcacctg atgcaaatag gactatgcgt gtaagttttg gtaacgtatt
      481 gccttataaa acagctgatg cagtaacata ttattcgcaa acaacttcat atggaattca
      541 gcaaaagtgg aatagtagtg ttaaagatta taggtataat gcaaggcttg atagtttatt
      601 acaacaaaaa caatatggga gttattctaa tgatacgtta tatataaatt ttgcagctac
      661 caatcatacc tcgggaggta attccggtag tcctgtgtta aatgcatatg ggaattttat
      721 tggattaaat ttcgatcgta ataaagaagg aactatgagt gatttttatt ttgatgaaca
      781 attttgtaga aatatttctg ttgatgtacg atatatatta ttttgcattg aaaaatatgg
      841 caatgcatac aatatattgc aagaattaga atttgtaaat cattaaaatt gagtaattat
      901 gagtgttaat atacatacac atgcaacaac aaataaatta ttattattta tagttgttcc
      961 tttagttttt tatattttaa aaacattaaa ttttattttt attccattat ttggagcatt
     1021 gcttgtagca ttgctttttt tgccgcttat gcgttatttt tcaaaaaagg agt
//
LOCUS       DAOTOW010000786         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_113116_length_1071_cov_3.282480, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000786 DAOTOW010000000
VERSION     DAOTOW010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113116_length_1071_cov_3.282480"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5243_11270"
     CDS             complement(<1..288)
                     /locus_tag="P5243_11270"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRS20076.1"
                     /translation="MKKTNILFVCLGNICRSPSAETIMKHLVQKKGLQDKYYIDSAGI
                     IGFHAGEPADSRMRAHAAKRGYTITSISRQFNPNTDFKEFDLIIGMDRKNIQ"
     gene            complement(334..>1071)
                     /locus_tag="P5243_11275"
     CDS             complement(334..>1071)
                     /locus_tag="P5243_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRS20077.1"
                     /translation="KLKDSFVLPPIVHRHILCIGIAEAKLAHVIAEWESQLPNCVHLA
                     YLPSPGIVKLRLTVIGEAIEHAKQIISEQESKILPIIQEFVYGYDDDTLEELIGSLLI
                     KKSATLSTAESCTGGNIASTIVKIPGSSQYFKGGIIAYSNDLKIQQLHISHDLLSNYG
                     AVSKEVVEAMAMNCRRIYNTDFAIATSGIAGPTGGTEEKPVGTVWIAVASATNICSQK
                     FLFGEHRERTILRTTIAALNLLQKNIE"
BASE COUNT          327 a          201 c          146 g          397 t
ORIGIN      
        1 ttgtatattt tttctatcca taccaataat taaatcaaat tctttaaaat cggtattagg
       61 attaaattgc cgagaaatac ttgttatagt atatcctcgc tttgctgcat gagctctcat
      121 tctggaatcg gcaggttcac cggcatgaaa tccgataata ccagcagaat caatataata
      181 tttatcttgc agaccttttt tttggactaa atgcttcatt atagtttctg ccgacggtga
      241 acgacaaata ttacccaaac acacaaataa aatatttgtt tttttcatcg tttttttatg
      301 tttttttgtt tttttatgtt atgcaaatat agattattct atattttttt gcaataaatt
      361 caatgctgct atagttgttc gcagaatagt tcgttcccta tgttctccaa acaaaaattt
      421 ttgactacaa atatttgtcg cacttgctac agcaatccat acagtcccca caggtttttc
      481 ttctgttcca ccggtgggcc cagcaatacc ggatgtagca attgcaaaat ctgtattata
      541 aattcttcta caattcatgg ccatagcttc aacaacctct ttactcacag ctccatagtt
      601 acttaataag tcatgagaaa tatgcaattg ttgtattttt aaatcattac tatatgcaat
      661 tattccacct ttaaaatatt gagaacttcc gggaattttt acaattgtag atgcaatatt
      721 tcctccggta caactttcag cagtactcaa tgttgcactt tttttaatta ataaagaacc
      781 tattaattcc tctaaagtat catcatcgta tccatataca aattcttgaa ttattggcaa
      841 tattttagat tcttgttcag aaattatctg ttttgcatgt tctattgctt cacctattac
      901 tgttaaacga agttttacaa ttccgggtga tggcaaatat gccaaatgca cacagttcgg
      961 caattgcgat tcccattctg ctattacatg agccaactta gcttcagcta ttccgataca
     1021 taaaatatga cgatgtacta ttggaggtaa aacaaaagaa tcttttaatt t
//
LOCUS       DAOTOW010000787         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_113127_length_1071_cov_2.910433, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000787 DAOTOW010000000
VERSION     DAOTOW010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113127_length_1071_cov_2.910433"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1071)
                     /locus_tag="P5243_11280"
     CDS             complement(<1..>1071)
                     /locus_tag="P5243_11280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRS20078.1"
                     /translation="FSRENDKLLRLFYKHHLPFCLTGAQVRVMSEIRADVESGNHMNR
                     LIQGDVGSGKTLIAILSMLMAIDNGHQACLMAPTEILAQQHYLSLQKLLGNLPISIAL
                     LTGSTKKKERDSIHQGLQNGTIHIVIGTHALLEDIVQFNNLGLCVIDEQHRFGVAQRA
                     RLWKKNSQTPHILVMTATPIPRTLAMTLYGDLDVSVIDELPPGRKPIQTILLRDSQRL
                     KMHGLLRQQIKEGRQAYIVYPLIQESEKLDYKDLEDGYASITQAFPLPNYSTTVVHGK
                     MKPQDKEYSMQLFAQGKADILVATTVIEVGVDVPNATVMVIESAERFGLSQLHQLRGR
                     VGRGGEQSYCILMAGNKLSNEG"
BASE COUNT          287 a          201 c          202 g          381 t
ORIGIN      
        1 ttaccttcgt tgctcaattt gttgccggcc ataagtatac aatacgattg ttctccaccg
       61 cgtccaactc tacctcgtaa ttgatgcaat tgtgataatc cgaatcgttc ggcactttct
      121 attaccatta ctgtggcatt gggtacatcg acacctactt caattacggt tgttgctacc
      181 aatatatcag cttttccttg tgcaaacagt tgcattgagt attctttatc ttgaggtttc
      241 attttgccgt gcactacagt agtagaatag ttgggcaggg ggaatgcttg agtaatagat
      301 gcatatccgt cttctaaatc tttataatca agtttttctg attcttgaat gagaggatac
      361 actatatatg cttgacgacc ttctttaatc tgttgacgaa gtaagccatg cattttgagt
      421 cgttgagaat cacgtaataa tatagtttgt attggttttc ttccgggtgg tagttcgtca
      481 attacagaaa catcaaggtc gccatacaat gtcatagcca atgtgcgtgg aattggggtt
      541 gctgtcatta ctaaaatgtg tggtgtttgc gaattttttt tccacaatcg agctcgttgt
      601 gctactccaa atctatgttg ttcatcaatt acacataatc ctaaattatt gaattgtaca
      661 atatcttcaa gaagagcatg tgtgccaatt actatatgta tagttccatt ttgtaatcct
      721 tggtgtatag aatcgcgttc ttttttctta gttgacccgg taagtaatgc tatagaaata
      781 ggtaaatttc ctaacaattt ttgtaacgac aaataatgtt gttgtgccag aatttctgta
      841 ggagccataa ggcacgcctg atgcccattg tcaatagcca taagcatact tagaattgct
      901 atgagtgttt ttccgcttcc tacatcacct tgaataagtc tattcatatg gttgcccgat
      961 tctacatcgg ctcgtatttc actcattact cttacttgag ctccggtaag gcaaaaaggt
     1021 aaatgatgtt tataaaaaag gcgtaatagt ttatcgtttt ctctgctaaa t
//
LOCUS       DAOTOW010000788         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_114179_length_1065_cov_4.626733, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000788 DAOTOW010000000
VERSION     DAOTOW010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114179_length_1065_cov_4.626733"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /gene="rlmN"
                     /locus_tag="P5243_11285"
     CDS             complement(<1..1026)
                     /gene="rlmN"
                     /locus_tag="P5243_11285"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRS20079.1"
                     /translation="MQKQDILSLSYSQILDICQEFGEKKFRVDQLYQWIWQKRVRNFS
                     EMTNLSKIFREQLQERFFFQTVKIDSIRSSTDGTQKVTFRLHDGCIIEGVVIPAKDRL
                     TACISSQVGCGLACAFCATGSLGFNRNLSVGEITDQYFVLDNLAREKHSQGITNIVYM
                     GMGEPLQNYDSVMLSIARLTEEKGQAMAPSRITISTSGLAKNIMKLADDGFKCNLAIS
                     LHTANNGIRNKLMPVNKANDLQKLSDAIAYFYDKTHTRISIEYTLLDGINDTIEHARE
                     LADFTKRFPVKINIIEYNPHQLSPYKKSDKQKTDSFVTFLEKLNLIVNIRYSKGKDID
                     AACGQLAA"
BASE COUNT          324 a          179 c          187 g          375 t
ORIGIN      
        1 tgcagcaagt tgtccacatg ccgcatcaat atctttgccc ttagaatacc gaatatttac
       61 tatcaaattt aatttttcta aaaatgtaac aaacgaatcg gttttttgtt tatcgctttt
      121 tttgtatggt gaaagttgat gaggattgta ttcaataata ttaattttta ccggaaagcg
      181 tttggtaaaa tcagctaatt cgcgagcatg ctctatagta tcgttaattc catcgagtag
      241 ggtatattct attgaaatac gagtgtgagt tttatcataa aaatatgcta tagcatcgct
      301 taatttttgt aagtcattag ctttgtttac cggcattaat ttgtttcgaa taccgttgtt
      361 tgcagtgtgt agcgatattg caagattaca tttgaatcca tcgtcggcaa gtttcattat
      421 gtttttagcc aaacctgagg tagatatggt aattctcgat ggtgccatag cttgaccttt
      481 ttcttctgtt aatcgagcta tagaaagcat taccgaatcg taattttgca aaggttcgcc
      541 catgcccatg taaacaatat tagtaatacc ttgcgaatgt ttttcacgag caaggttgtc
      601 aagtacaaaa tattgatcgg taatttcgcc aacacttaaa tttcggttaa aacctaaact
      661 gccggttgca caaaaggcac aagctaaacc acagcctact tgcgacgata tacatgctgt
      721 aagtctatct tttgccggaa ttaccacacc ttctataata caaccgtcat gcaagcggaa
      781 tgttactttt tgagttccat cggtacttga acggatacta tctattttaa cagtttgaaa
      841 aaagaaacgt tcttgtaatt gttctctgaa aattttcgaa agattagtca tttcggaaaa
      901 attgcgaact cttttttgcc aaatccattg atacaattga tcaacacgaa attttttttc
      961 accaaattct tggcatatgt caagaatttg tgaatatgag agagaaagaa tatcttgttt
     1021 ttgcatggtg tgattcaatt gtacattata taatagtatt aaggt
//
LOCUS       DAOTOW010000789         1064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_114428_length_1064_cov_2.368682, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000789 DAOTOW010000000
VERSION     DAOTOW010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114428_length_1064_cov_2.368682"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>1064
                     /locus_tag="P5243_11290"
     CDS             106..>1064
                     /locus_tag="P5243_11290"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRS20080.1"
                     /translation="MKQLLSVVLLIVLLSPAKADEGMWLLTMLDKLELQKKGCKLTPE
                     QIYNINKSSLKDAIIGLGESDNPYNFFCTAELVSSQGLVFTNYHCGFDMIQKHTSIAN
                     NYIDNGFWAKNKNEELRNDGITATIVVRMIDVSDKIMPLYKIGITNKDKLEDTISKVS
                     AIIEKSVSDTSHYKGSVKGFFENNQYFLFIYETFLDVRLVGAPPRSIGKFGGDTDNWM
                     WPRHTGDFCVLRIYSDTNNKPKTYSQDNIPLTPRYFLPVSLQGYNNGDYAMVMGFPGS
                     TDRYKTATGIEGLYTFNNAAIIAIGDTMLDVYKQYMKSSAAIK"
BASE COUNT          398 a          167 c          173 g          326 t
ORIGIN      
        1 aaaaattatc atcaatttta gcagataaaa tcgttgcatt ttttagtaaa aaataatgta
       61 tatttgaatg cgattttcaa tcactattca aaatactatt aaaacatgaa acaattatta
      121 tctgttgtac tacttatagt acttttaagc ccggcaaagg ctgatgaagg catgtggtta
      181 ctcacaatgt tagacaaatt agaattacag aaaaaaggct gcaaacttac accggaacaa
      241 atttataata taaacaaaag ttcactaaaa gatgcaataa taggattagg cgaaagtgac
      301 aatccatata attttttctg cacagctgaa cttgtttcat ctcaaggatt agtatttacc
      361 aattaccact gtggttttga tatgattcaa aaacacacaa gtatagcaaa taactatatt
      421 gataatggat tttgggcaaa aaataaaaat gaagaactca gaaacgatgg cattacagca
      481 actattgtag tacgcatgat tgatgtaagt gataaaatta tgcctctata taaaattggc
      541 attacaaata aagataaact ggaagataca ataagtaaag tttctgcaat cattgaaaaa
      601 agtgtttcag acacttcaca ttataaaggt tcggtaaaag gtttttttga aaacaatcaa
      661 tattttctat ttatatacga aacatttctt gatgttcgtt tagttggtgc tcctcctcgc
      721 agtataggta aatttggcgg cgataccgat aattggatgt ggccacgaca cacaggcgat
      781 ttttgtgtat tacgaatata cagtgataca aataacaaac ccaaaaccta ttcacaagat
      841 aatataccgc ttactccacg atatttttta cctgtttcgt tgcaaggata caacaatggc
      901 gattatgcta tggttatggg attccccggt agtaccgacc gatacaaaac agctaccggc
      961 attgaaggat tatatacatt taataacgct gccataattg ctataggtga tacaatgctt
     1021 gatgtttaca aacaatatat gaaatcaagt gctgccataa aaat
//
LOCUS       DAOTOW010000790         1063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_114534_length_1063_cov_3.025794, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000790 DAOTOW010000000
VERSION     DAOTOW010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114534_length_1063_cov_3.025794"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="P5243_11295"
     CDS             complement(<1..659)
                     /locus_tag="P5243_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRS20081.1"
                     /translation="MTHFALQSYKNSILYSIIYMIFGTFIALALQHIITICFVKIFIV
                     AIVYTLSFAGLSIPLWMYVQYVDNAAQKELQKIINTCAVFILFQTIVLTLTLVSTFIV
                     LHTEEQQSILLSMPILGIISSIGFGFIYQLYRNKKEKLIHQEPEKKYISDTLNTEQSQ
                     PQTDIIERISVKHGQKIHVIPVETIQYIQAEGDYVMIYTNDSKYLKEQTMKYFESHLS
                     Q"
     gene            complement(672..>1063)
                     /locus_tag="P5243_11300"
     CDS             complement(672..>1063)
                     /locus_tag="P5243_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HRS20082.1"
                     /translation="HTHCKFHNHAQKSTWENTNGYVYKNSSFQTSEHIVLDPEFKGGD
                     LQISFGETTLDLRKTTLSNEQVILNIKISFGSIIIYVPYNWNIQPQVDAAFGGFEDKR
                     YGAEASTDNKSLIIRGNVTFGNAELRN"
BASE COUNT          332 a          146 c          170 g          415 t
ORIGIN      
        1 tcttgtgaaa gatgagattc aaaatatttc attgtttgtt cttttaaata tttagaatcg
       61 ttggtgtaaa tcattacata atcaccttct gcttgtatat attgaatagt ttctaccggt
      121 ataacatgaa ttttttgtcc atgttttacg ctaattcgtt caataatgtc tgtttgtggt
      181 tgtgattgtt ctgtatttag agtatctgat atatattttt tttcaggttc ttgatgtatt
      241 aatttctctt ttttattacg gtatagttga tatataaaac caaatcctat actgcttatg
      301 atgcctaata ttggcattga gagcagtata ctttgttgtt cttctgtgtg taaaactatg
      361 aatgtactaa ccaaagtaag agtaagtact atggtttgga ataaaataaa aacagcacat
      421 gtatttatta ttttttgtag ttctttctga gcagcattat caacatattg tacatacatc
      481 catagtggaa tacttagtcc tgcaaatgat agtgtatata ctattgctac tataaatatt
      541 ttgacaaagc aaattgtaat aatgtgttgc aacgctagtg ctatgaatgt gccaaaaatc
      601 atataaataa tactatacag tattgaattt ttatatgatt gtaatgcaaa atgtgtcatg
      661 aatgcttgta attaattacg taattcggca tttccgaatg ttacattgcc acgaataatc
      721 aatgatttat tatcggtaga agcttcggct ccgtatcgtt tatcttcaaa cccaccaaaa
      781 gctgcatcta cttgaggttg tatattccaa ttgtatggta catatattat aatagaacca
      841 aatgaaattt ttatatttaa tattacttgt tcatttgata atgtagtctt acgcaaatca
      901 agggtagttt cgccaaacga aatttgtaaa tcacctcctt taaattcggg gtcaagcact
      961 atgtgttccg aggtttgaaa cgaagagttt ttatatacat atccgttggt attttcccaa
     1021 gtactttttt gagcatgatt atgaaattta caatgagtat gta
//
LOCUS       DAOTOW010000791         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_115122_length_1060_cov_2.937313, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000791 DAOTOW010000000
VERSION     DAOTOW010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115122_length_1060_cov_2.937313"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="P5243_11305"
     CDS             complement(<1..901)
                     /locus_tag="P5243_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRS20083.1"
                     /translation="MDTAIHTEKETWGTYQKHVADDNYYYARSCIRQNFFPASEYMFL
                     HILRKVLGKHVYDDPNHTTCTGIGYHSDIVPFETIQTVVARHFALMTEAGLENIAISC
                     VTSFGIYTEILETWQHFPEQLEKTREFLYKATKREFNLPKNIAHTSDIIYKFRHEIAE
                     KAVYKLVHAQTGEPLNVVEHIGCHYAKMFPTKGIGGAEFPQVLSGMITAWGGKVIDYP
                     ERRHCCGFGFRNYLVQANRGYSVSNTYKKLESMQPYNPDFILTNCPGCAMFIDKWQYT
                     IAEMEQKTFDSQGKGIPVLTYEEL"
BASE COUNT          321 a          203 c          154 g          382 t
ORIGIN      
        1 caagctcttc atacgttaac acaggaatac ccttgccttg agagtcaaat gttttctgtt
       61 ccatttctgc aatagtatat tgccatttat caataaacat agcacaacct gggcaattag
      121 taagaataaa atcggggtta tacggttgca ttgattccaa ttttttatac gtatttgata
      181 cagaatatcc tctatttgcc tgcaccaaat aattcctaaa tccaaaaccg caacaatgtc
      241 gtcgctctgg ataatcaatt actttaccgc cccatgcagt aatcattccc gaaagaactt
      301 gaggaaactc tgcgccacca attcctttag taggaaacat ttttgcatag tgacaaccaa
      361 tatgttcaac aacattgagc ggctcacctg tttgtgcatg tactaattta taaactgctt
      421 tttctgctat ttcatgtctg aatttataaa ttatatcact agtgtgagct atattttttg
      481 gtaaattaaa ttctcgtttg gttgctttat ataaaaattc tcttgttttt tctaattgtt
      541 ccgggaaatg ttgccatgtt tctaatattt ctgtatatat gccaaacgat gttacacatg
      601 agatagcaat attttctaac cctgcttctg tcatcaaagc aaaatgacga gcaacaacag
      661 tttgtatagt ttcaaacgga actatatccg aatgatatcc aatgccggtg cacgttgtat
      721 gattagggtc atcatataca tgtttcccta acaccttacg caatatatgt aaaaacatat
      781 attccgatgc aggaaaaaaa ttttgcctta tacaactacg tgcgtaataa taattatcgt
      841 cggcaacatg tttttgatat gttccccacg tttctttttc tgtatgtatt gcagtatcca
      901 ttattctgaa tgtgtattac tattatatgt ataaacgtgt ttgaaatatt cattatcaat
      961 attatcacta aaccccaaat tcattcgttc agcttgtttt tttgattctt cctcaatgat
     1021 agtatataat tcggttccat ttgtaacatc aaatattgct
//
LOCUS       DAOTOW010000792         1054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_116228_length_1054_cov_2.451451, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000792 DAOTOW010000000
VERSION     DAOTOW010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116228_length_1054_cov_2.451451"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..>1054
                     /locus_tag="P5243_11310"
     CDS             113..>1054
                     /locus_tag="P5243_11310"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRS20084.1"
                     /translation="MAKKIGILTAGGDCPGLNAAIRGVAKTAIVEYGMEVIGFSSGFK
                     GLIEKEYMVLDEKSVSGILTLGGTILGTSREKPFKKVNPDDMEEDTKTQQIIDNYNQL
                     GLDCLVCIGGNGTIKNAYKLSQIGLNIIGIPKTIDNDVWGTDRTFGFDSAVAICTDAI
                     DKLHTTGNSHKRIMVIEVMGHNAGWIALYAGIAGGGDVILIPEIEYDLQIIAKYLADR
                     HAKGKPVSIVVAAEGVKHGKKKSPGPYLAKKIEELTGIETRETVLGYIQRGGTPTATD
                     RILATRYGRYAAELIATEQYNQMVAFKDNVIISIPLAE"
BASE COUNT          361 a          146 c          241 g          306 t
ORIGIN      
        1 catattggcc ccggtgattt tattcaggca tgggaagtat ggactattga agaatacaac
       61 ggtgataaca ccgaatcatc acatatatca tttattaaat cattgtaaat ctatggcaaa
      121 gaaaattgga attttaacag caggcggcga ttgtccggga ttaaatgcag caatacgcgg
      181 tgttgccaaa actgctattg tagaatatgg aatggaagta ataggttttt catcgggatt
      241 taaagggctg atagagaaag aatatatggt gcttgatgaa aaatcggttt cgggaattct
      301 tacgttagga ggtaccatat tgggaacatc acgggaaaaa ccatttaaaa aggtaaatcc
      361 cgatgatatg gaagaagata ctaaaacaca acaaattata gataattata atcaattggg
      421 attagattgt ttggtatgta taggaggaaa tggtacaata aaaaatgcgt ataaattatc
      481 gcaaatagga ttgaatataa taggtatacc caaaactatt gataatgatg tgtggggaac
      541 cgatagaaca tttggttttg attcggctgt agctatatgt accgatgcta ttgataaatt
      601 gcatactacc ggtaattcgc ataaacgtat tatggtaata gaagtaatgg ggcacaatgc
      661 tggttggatt gctttgtatg ccggtatagc aggtgggggt gatgttattt taatacctga
      721 aatagagtat gatttgcaaa taattgcaaa atatttggct gacagacatg ctaaaggtaa
      781 accggtttct attgttgttg cagccgaagg ggttaaacat ggtaaaaaga aatcgccggg
      841 tccgtatttg gcaaaaaaaa ttgaagaact taccggtatt gaaactcgtg aaacggtatt
      901 aggatatatt caacgcgggg gtacacctac agcaaccgat agaattcttg ctactcgata
      961 cggacgttat gctgccgagc ttatagctac cgaacaatat aatcaaatgg ttgcttttaa
     1021 agataatgtt attattagta ttccacttgc cgaa
//
LOCUS       DAOTOW010000793         1053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_116378_length_1053_cov_3.405812, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000793 DAOTOW010000000
VERSION     DAOTOW010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116378_length_1053_cov_3.405812"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="P5243_11315"
     CDS             <1..558
                     /locus_tag="P5243_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRS20085.1"
                     /translation="AKTRLGWDATDMPIVRVAEQLQDVGIQALTIHGRTRAQMYTGEA
                     DWSLIRQVKQNARMHIPIIGNGDIDSPQKAKQAFDEYGVDAIMIGRASIGAPWIFRDV
                     KHYLHTGELLPTMNVQQQVDELKEYLLKSVEVKGLPGGVIHMRRQFAKAFKGLDNFRE
                     TRIQLLTRTDLQETIQLLDNIANRW"
     gene            820..1029
                     /locus_tag="P5243_11320"
     CDS             820..1029
                     /locus_tag="P5243_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20086.1"
                     /translation="MLCSVFVFSQTTNDYRTNGDGDFDNAGIWQCYNSTTWVAAATAP
                     TNRSTAGTITIRTGHTVNHCCPIKN"
BASE COUNT          363 a          180 c          203 g          307 t
ORIGIN      
        1 gcaaaaactc gtttgggatg ggacgctacc gatatgccta tagtgcgtgt agcagaacaa
       61 ttacaagacg taggcataca ggcacttaca atacatggac gaacacgagc tcaaatgtat
      121 accggcgaag ctgattggtc gttgatacgc caagtgaaac aaaatgcacg catgcatata
      181 ccaattattg gcaacggaga tattgatagc ccccaaaaag ctaaacaagc ctttgatgaa
      241 tatggggtag atgctataat gataggacgt gcaagtatag gagctccatg gatttttcgc
      301 gatgtaaaac actatttaca taccggagag ctcctcccca ccatgaatgt tcaacaacaa
      361 gtagacgaac tcaaagaata cttactaaaa tctgtagaag taaaaggtct acccggaggc
      421 gtgattcaca tgcgtcgtca gtttgccaaa gcctttaaag gactcgacaa ttttagagaa
      481 actcgcatac aattactcac gcgaactgac ttgcaagaaa ccatacaatt gcttgataat
      541 attgcaaata gatggtagtg atacgctgcg tataaatact attttttcgt tttaatttaa
      601 tttcattctt catagtatat aggggattca ggacatttat tgttttataa acaatattta
      661 actaaaaatg tattgatttt tgtataacaa taattgtatc tttacgtaac tttttgggaa
      721 ttttgagata ggaattacat atttatgata tatttcacaa aacacatata ttctttttgg
      781 gcatatacac agaaacgcat tatactaata atggtaagtt tgttgtgtag tgtttttgtg
      841 ttttctcaaa caacaaatga ttacagaact aatggagatg gagattttga taatgccggt
      901 atttggcaat gctataatag tactacatgg gtagcagctg ccactgcacc tacaaacaga
      961 tcaacagcag gaactattac gataagaaca ggacatactg taaatcactg ttgtccgata
     1021 aaaaattaag agaaggaaga tgttagtctt cct
//
LOCUS       DAOTOW010000794         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_117238_length_1049_cov_1.535211, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000794 DAOTOW010000000
VERSION     DAOTOW010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117238_length_1049_cov_1.535211"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..906)
                     /gene="nifH"
                     /locus_tag="P5243_11325"
     CDS             complement(82..906)
                     /gene="nifH"
                     /locus_tag="P5243_11325"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HRS20087.1"
                     /translation="MRKVAIYGKGGIGKSTTTQNTVAGLAEMGKKVMVVGCDPKADST
                     RLLLGGLAQKTVLDTLREEGEDVELEDVLKPGYRNTSCVESGGPEPGVGCAGRGIITS
                     INLLEQLGAYEEDKSLDYVFYDVLGDVVCGGFAMPIRDGKAEEIYIVVSGEMMAMYAA
                     NNICKGIVKFAQAGTVRLGGLICNSRKVDNEAQMIEEFAKRLGTQMIHFVPRDNMVQR
                     AEINRKTVIDYDPVHPQADEYRALAKKIDENKLFVVPKPLSIPELEQLLIEYGLFN"
BASE COUNT          289 a          232 c          158 g          370 t
ORIGIN      
        1 ataatgcttt cattactact gccgattttt cggggcgtac tatagctctt aataaataca
       61 tactgttgtc ctccttagtt tttagttaaa taatccatat tctattaata attgctccaa
      121 ttcaggaatt gaaagtggtt ttggcactac aaataatttg ttctcgtcta tttttttagc
      181 caatgctcta tattcatctg cttgtggatg taccggatcg taatcaatta cagtttttct
      241 gttgatttct gcacgttgaa ccatattgtc gcgtggtaca aagtgaatca tttgtgtacc
      301 taagcgttta gcaaactctt ctatcatttg agcttcattg tcaacttttc ggctgttgca
      361 aataagacca cctaaacgaa ctgttcctgc ttgtgcaaat ttcacaatac ctttacaaat
      421 gttgttggca gcatacattg ccatcatttc gcccgatact acaatgtata tttcttcggc
      481 ttttccatcg cggattggca ttgcaaatcc accgcatacc acgtcaccaa gtacgtcgta
      541 aaatacatag tcaagacttt tgtcttcttc gtatgcacca agttgttcca ataagttgat
      601 agatgtaata attccgcgac ctgcacaacc tactcccggt tcagggccac ccgattccac
      661 gcaacttgta ttgcgatatc ccggttttaa tacatcttct aactctacat cttcaccttc
      721 ttcgcgaagt gtatccaata ctgttttttg agcaagacca cctaagagta atcgagttga
      781 atcggcttta ggatcgcatc ctactaccat aactttttta cccatttctg ctaatcctgc
      841 aactgtgttt tgtgtagtcg tacttttgcc gattccacct ttaccataaa tagctacttt
      901 tctcattttg tataacctcc taattatttt ttagttttct ctctattttt cagctatgat
      961 gccacaaaaa aaaatagcag ttttgctcac aaaaaaatgg atgcaacacg ttgatattca
     1021 agatgaaaaa aatcaaaaaa aattgcaat
//
LOCUS       DAOTOW010000795         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_117813_length_1045_cov_3.275758, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000795 DAOTOW010000000
VERSION     DAOTOW010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117813_length_1045_cov_3.275758"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..>1045
                     /locus_tag="P5243_11330"
     CDS             328..>1045
                     /locus_tag="P5243_11330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS20088.1"
                     /translation="MVTVYTLRCFQFAKKCIVIPLLFISYSIFAQAPVANFSASATSG
                     CGVTVIIFEDLSTNNPTSLTWNFGIGGNQTPELRADRKYSFIYSTPGTYTVRLTATNA
                     SGSNTMEKTAYIRIYANPTANFTISQTAQCQGDLLQFNDASTSDVAITSWAWNFGDGN
                     VATIQNPTHTYANTGTYSVFLSITNANGCTAFYNNAAAVSISTKPTAAFTASATEGCT
                     APFTTTFNSGASTGPITSYLW"
BASE COUNT          353 a          172 c          169 g          351 t
ORIGIN      
        1 tgtacaatac attatctgtt tctgtgaaaa tctgagtaat ctgtggtgaa aagatgacta
       61 attatctatt attttaaaaa catgtaagtt tcttgtattc agaattttgc aaattttata
      121 cttttattga cgcaaaaatt ttgcattaaa aaacaaaaaa cctctcaaaa attttgtatt
      181 tcatgaaaaa aacttgtaat tttcaacata atttggcgat aaagggattg tttcttaatt
      241 ttttgagagt gtagtgaaat tcatgattca atctttaatc ggcaactata tgaatagatt
      301 gaataagaac aatttaaacc tttgcttatg gttacagtat atacgctccg ttgttttcag
      361 tttgcaaaaa aatgtattgt aattcctttg ttatttatta gttatagtat atttgctcaa
      421 gcaccggttg caaacttttc agcatcggca acatcgggtt gtggtgttac agttataatt
      481 tttgaagact tatcaactaa taatcctaca agtcttacat ggaattttgg tatagggggt
      541 aatcaaactc ccgaattacg agccgacaga aaatattcgt ttatatattc tacaccggga
      601 acatatactg tacgacttac tgcaacaaat gcatcgggta gtaacacaat ggaaaaaaca
      661 gcatatattc gaatttatgc aaatcctaca gctaatttta ctatatcgca aacagcacaa
      721 tgccaaggtg atttacttca atttaatgat gcatcaactt ctgatgtcgc aataactagt
      781 tgggcgtgga attttggtga tggaaatgtt gcaactattc aaaatcctac ccatacctat
      841 gccaatacag gaacttatag tgttttttta tcaattacaa atgcaaacgg gtgtacagca
      901 ttttacaata acgcagcagc tgtaagtatt tctacaaaac ctactgcagc attcactgca
      961 agtgcaaccg aaggttgtac ggcaccattt actacaacat tcaatagtgg agcatctacc
     1021 ggacctatta caagttattt atgga
//
LOCUS       DAOTOW010000796         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_118841_length_1040_cov_2.491371, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000796 DAOTOW010000000
VERSION     DAOTOW010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118841_length_1040_cov_2.491371"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="P5243_11335"
     CDS             <1..874
                     /locus_tag="P5243_11335"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS20089.1"
                     /translation="DYFYVKVKQADGQEAITSPIWISGGASNQAPTCSISSPASSTSY
                     SAPATITIQAIATDSDGTIAKVEFYQGSTKLGEDTSSPYSYTWSNVAAGSYSITAKAT
                     DNLGAVTTSSAVTVTVTTSGGSTPITVSKRIATGMDDVEESQTGSMYTNSSDIELVYD
                     ESTYGNQVIGLRFTGLNIPQGAIISKAYIQFTCDEKKTAATSVTIRGEAADNSAAFTT
                     ASKNVSNRTKTTASVAWNNIPAWNTVDAATTNERTPELQTIVQEIVNRTGFTSSSAMT
                     FIITGTGTRTAHAY"
BASE COUNT          358 a          192 c          187 g          303 t
ORIGIN      
        1 tgattatttt tatgtgaaag taaaacaagc tgatggtcaa gaagctatta catcgcctat
       61 ttggatatcg ggaggtgctt ctaatcaggc tcctacatgt tctatctcaa gtcctgcttc
      121 cagcacaagt tattcagctc cagcaactat tactatacaa gcaattgcta ccgattctga
      181 cggaacaata gcaaaagtag aattttatca aggttctact aaattaggcg aagatacatc
      241 atcaccgtat tcatatactt ggtcaaacgt tgctgcaggt tcatatagta taaccgcaaa
      301 agcaaccgat aatcttggag ctgttacaac ctcatcggct gttactgtta cggtaacaac
      361 ttcaggcggt tctactccaa taactgtatc taaacgtatt gctacaggta tggacgatgt
      421 agaagaatca caaacaggca gtatgtatac aaacagttct gatatagaat tggtatatga
      481 cgaatctaca tatggaaatc aagtaattgg tttacgtttt acaggattaa acattccaca
      541 aggagcaata atttctaaag catatattca gtttacgtgt gacgaaaaga aaacggctgc
      601 tacaagtgta actattcgtg gtgaagctgc tgataattca gctgcattta caactgcaag
      661 taaaaatgta tcaaaccgaa caaaaacaac tgcatcagta gcttggaata atattcctgc
      721 atggaataca gtagatgctg caactactaa tgaacgaact cctgaattac aaacaattgt
      781 tcaagaaatt gtaaatcgta caggctttac ttcatcaagt gctatgacat ttattattac
      841 cggaacagga acaagaactg ctcatgcata ttaaggttct tctgctaagg ctccacttct
      901 atatatagaa tatactgtag gaggtcttaa aagtgaagtt ttatttgaat catattctga
      961 cgataatact gtaatagaaa cggaacaaac ttcaactccg gaacaaataa aagtatatcc
     1021 taatcctgtt gtggatgttc
//
LOCUS       DAOTOW010000797         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_118850_length_1040_cov_2.321827, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000797 DAOTOW010000000
VERSION     DAOTOW010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118850_length_1040_cov_2.321827"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="P5243_11340"
     CDS             complement(<1..>1040)
                     /locus_tag="P5243_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRS20090.1"
                     /translation="KNRKRILSWARSSHDNFGNKLALIDAEYARVTQDFKKASEMYDK
                     AIRLAQEQKILHEEALANEYAGKFWESQCKSDFACMYIYRAYKLYEIWGAHVKTEQML
                     LNFETYIHQSQTHDISNTTHVTQSHSTTKNTQASSIDIETVMDAAKTISQEIVLNSLF
                     EKILTIIIEHACAEKGVLLLQDSTVSSKFLLEAEGILQDNTVNITHYNSEPTEKQIPL
                     SIIRYIIRSQETVILHNAHQEGTFIRDEYIKNNQTKSIVAIPILHQSKLSGILYLENN
                     QISDVFNEQKIGILSLLCTQAAISIENARLYNSLELKVIERTKEVVEQKEIIEYKNKE
                     ITDSIQYAKQIQT"
BASE COUNT          294 a          151 c          159 g          436 t
ORIGIN      
        1 gtttgtattt gtttcgcata ttgaatacta tcagtaattt ctttgttttt atattcaatt
       61 atttcttttt gttcaactac ttcttttgtt cgttctatta cttttaattc cagtgaattg
      121 tatagtcgag cgttttcaat agaaatagca gcttgtgtac acaaaagtga taagattcca
      181 atcttttgtt cattaaaaac atcacttatt tgattatttt ctaaatataa tattcctgaa
      241 agtttagatt gatgcaaaat aggtattgca acaattgatt ttgtttgatt attttttatg
      301 tattcatctc gaataaatgt accttcttga tgtgcgttat gcaaaataac agtttcttgt
      361 gatctaataa tatatcgaat tatactcaaa ggaatttgtt tttctgtagg ttcagaatta
      421 taatgagtta tattaactgt attatcttgt aatattcctt cagcttcaag taagaatttt
      481 gaagaaactg ttgaatcttg cagcaataat actccttttt cggcacaagc atgttcaata
      541 atgattgtta atattttttc aaatagcgaa tttagaacta tttcttgtga aatagttttt
      601 gcagcatcca taactgtttc tatgtcaata ctcgatgctt gagtgttttt ggtagtcgag
      661 tgtgattgtg taacatgtgt tgtgttgctt atgtcatgtg tttgagattg atgaatatat
      721 gtttcgaaat tgagaagcat ttgttctgtt tttacgtgtg ctccccaaat ttcgtatagt
      781 ttatacgctc tgtatatata catacacgca aaatcagatt tacattgcga ttcccaaaat
      841 ttgccggcat attcattagc taaagcttct tcatgtaata ttttttgttc ttgagccaat
      901 cgaatagctt tatcatacat ttcgcttgct tttttaaagt cttgagttac tcgtgcatat
      961 tctgcatcta ttaaagcaag cttattacca aaattgtcat gtgaactccg agcccaagat
     1021 agtattcgtt tcctattttt
//
LOCUS       DAOTOW010000798         1037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_119413_length_1037_cov_2.323829, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000798 DAOTOW010000000
VERSION     DAOTOW010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119413_length_1037_cov_2.323829"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="P5243_11345"
     CDS             <1..791
                     /locus_tag="P5243_11345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS20091.1"
                     /translation="SRKSIARLQMTQKKLIESEKMASLGNLVAGVSHEINTPVGIGIA
                     ASTSLKSKTEDILELFTTRKMKQSDLDTYLQSTQNAVQLIQSNLKRTGDLVKSFKRVS
                     VDEMTDTMREFNLLEYIGEIVINHDAKLKERNIELRIDCPSDISIYSYPGAFAQIISN
                     MTSNALLHAFKNKEPGIITITCRTANNRLYFIFSDNGCGMTNEVVQKVFNPFFTTNMQ
                     AGTGLGMNIVYNLVTQKLQGDIYCESTVNEGTTFKIDIPLKEQS"
     gene            805..>1037
                     /locus_tag="P5243_11350"
     CDS             805..>1037
                     /locus_tag="P5243_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS20092.1"
                     /translation="MNCIILDDDIVICTLLENYIKKTPGIHIKHVFHSPLQAFESINE
                     FSHIDVLFLDMEMPDMNGIEFLNSLIHIPQIIF"
BASE COUNT          405 a          152 c          158 g          322 t
ORIGIN      
        1 gcagtagaaa aagcattgca agattacaaa tgacacagaa aaaactcatt gaatcagaaa
       61 aaatggcttc attaggaaat ttagtggcag gagtttcgca tgaaataaat actcctgtgg
      121 gtattggtat agctgcatct acatctctta aatcaaaaac cgaagatatt ttagaattgt
      181 ttactacccg caaaatgaaa caatctgatt tagatacata cttacaatca actcaaaatg
      241 cggttcaact cattcaatct aatttaaaac gtaccggcga tttggtaaaa agtttcaaac
      301 gagtgtcggt agatgaaatg acagatacaa tgagagaatt taatttactt gaatatattg
      361 gagaaattgt aataaaccat gatgcaaaac tcaaagaacg taatatagaa ttacgtattg
      421 attgtccttc tgatatttca atatacagct atccgggagc atttgcacaa attatatcaa
      481 atatgacatc aaatgcatta ttacatgcgt ttaaaaacaa agaaccagga ataattacaa
      541 ttacatgtcg cacagcaaat aatagattat attttatatt ttctgacaat ggatgtggaa
      601 tgacaaacga agttgtacaa aaagtattta accccttctt tacaacaaac atgcaagcag
      661 gaacaggttt aggaatgaac atagtatata atttagtaac tcaaaaactg caaggtgata
      721 tatattgcga aagtacagta aatgaaggca ctacattcaa aattgatata ccattgaaag
      781 aacaatcgta aaacttgtat atatatgaac tgcataattt tagatgatga tatagtaatt
      841 tgcacgttac tagaaaacta tattaaaaaa actccgggta ttcacataaa acatgtattt
      901 catagtcctt tacaagcatt tgaatctatt aatgaatttt cacatattga tgttctgttt
      961 cttgatatgg aaatgcctga tatgaatggc attgaatttc ttaattctct tatacatatt
     1021 cctcaaatta tttttat
//
LOCUS       DAOTOW010000799         1035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_119766_length_1035_cov_3.087755, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000799 DAOTOW010000000
VERSION     DAOTOW010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119766_length_1035_cov_3.087755"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1035
                     /locus_tag="P5243_11355"
     CDS             <1..>1035
                     /locus_tag="P5243_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS20093.1"
                     /translation="KNAEIIDGKTIIGILLINTVWAATIQGFVYDKDNKEPLIGATVS
                     IEQLKEYTVTGLDGSFIFKNIEAGTYYISISYLGYKSITQKAIIENNNIPIKFNFLLE
                     TEHTMLQSVTIVAHGAQNTDANARLSEKTSMQVINVVSASSIQQSPDLNIANVVQRIS
                     GVTLEKNSSGEAQYAILRGMDKRYNYTLVNGLKISSPHNKHRFVPLNIFPSDLADRVE
                     VYKTLTADMEGDGSGGVINLVMKDSPNAFFVNTNLGIGYNSYFIQNGLSTYPRSHISM
                     LSPREQNGKTYTASLTDLNRDLGKISETNPLPNSNIGVALGNRFLQKKLGIMVAANYS
                     NLSKGNQSVI"
BASE COUNT          392 a          151 c          179 g          313 t
ORIGIN      
        1 taaaaatgct gaaataatag acggaaagac aataattgga attttattaa taaacactgt
       61 ttgggcagca actattcaag gatttgtgta cgacaaagac aacaaagaac cgctgatagg
      121 agctaccgta agtattgaac agcttaaaga atatacagta acaggtttag acggctcatt
      181 tatatttaaa aatatagaag ccggtacata ttatatatca atatcgtatt taggatataa
      241 aagtataact caaaaagcaa ttattgaaaa caataatata cctattaaat tcaatttctt
      301 gcttgagaca gaacatacta tgttgcaatc ggtaacaata gtagcacatg gagctcaaaa
      361 taccgatgca aatgctcgcc tgagtgaaaa gacttcaatg caagttataa atgttgtaag
      421 tgcttcatct attcaacaat cgccagattt aaacatagca aatgtagtac aacgtatatc
      481 gggggtaact cttgagaaaa acagttcggg tgaggctcaa tatgcaatat tgcgaggaat
      541 ggataaacga tataactata cattagttaa tggtttgaaa atttcgagtc cccataacaa
      601 acatagattt gtgccattaa atatttttcc aagtgattta gccgaccgag ttgaagtata
      661 taaaactcta acagccgata tggaaggtga tggttctggt ggtgtaataa atcttgttat
      721 gaaagattct cccaatgctt tttttgtaaa taccaattta ggaattggat ataactcgta
      781 ttttatacaa aatggattat caacatatcc tcgttcacat atttcaatgt tatcgccccg
      841 tgaacaaaat ggaaaaacct atacagcatc tttgactgat ttaaatagag atttaggaaa
      901 aataagtgaa acaaatccat tacccaattc aaatattggt gttgcattgg gtaatagatt
      961 tttacaaaaa aaattgggaa taatggttgc tgctaattat agtaatcttt caaaaggaaa
     1021 tcaatcagtt atttt
//
LOCUS       DAOTOW010000800         1034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_120046_length_1034_cov_1.977528, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000800 DAOTOW010000000
VERSION     DAOTOW010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120046_length_1034_cov_1.977528"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="P5243_11360"
     CDS             complement(<1..1001)
                     /locus_tag="P5243_11360"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRS20094.1"
                     /translation="MNISIEGFTNSNSVEVLFNENPAIVIQTNANTLASLKSQGIECI
                     ELGTVTSNNKLTIQTKTETLVFDIPELRKTWYTTSYLLDCEQSGNKKALERFETFAAT
                     PLKYTFPKHFKGNEQAYNLNLYRTKPSGIRAAIIREKGVNGDREMAWCLHAAGFDVKD
                     VHMTDLIHGRETLQDISMIVFVGGFSNSDVLGSAKGWAGAFLYNPKAKQALDAFYARP
                     DTMSLGVCNGCQLVIELGLLHQNHDKKPKMLHNDSHKFESNFIGVKIKPSSSIMLKSL
                     ENTELGIWIAHGEGKFNLPNDESSYQIALQYSYNSYPANPNGSDYNTIVGNGLDLDP"
BASE COUNT          305 a          183 c          168 g          378 t
ORIGIN      
        1 ttagggtcta aatcgagtcc gttaccgact atcgtgttat aatcgcttcc gttaggattt
       61 gccgggtaac tgttgtatga atattgtaat gctatttgat acgaagattc gtcattcggt
      121 aaattaaact taccttcgcc atgtgctatc catataccaa gttcagtatt ttcaagtgat
      181 ttaagcataa tactgctact tggtttaatt ttcacaccta taaaattcga ttcaaattta
      241 tgagaatcat tatgaagcat tttgggtttt ttatcatgat tttgatgaag taaccctaat
      301 tcaataacaa gttgacaacc attgcaaaca cctaaactca ttgtgtcggg gcgagcataa
      361 aatgcatcta atgcttgttt tgctttagga ttatataaaa atgctcctgc ccaaccttta
      421 gccgagccta atacgtctga atttgaaaat cctccaacaa atactatcat tgaaatgtct
      481 tgtaatgttt cacgtccatg tatcaaatcg gtcatgtgta catcttttac atcaaatccg
      541 gcagcatgaa gacaccatgc catttctcta tcgccattaa cacctttttc gcgaattata
      601 gctgcacgaa taccgcttgg tttggttcta tataaattta gattatatgc ttgttcattt
      661 cctttaaaat gtttggggaa tgtatatttt agtggagtag cagcaaatgt ttcaaagcgt
      721 tctaatgctt ttttattacc actttgctca caatcgagca agtatgaagt tgtataccac
      781 gttttacgaa gttccggaat atcaaataca agagtttctg tttttgtttg aattgtaagt
      841 ttattattag aagtaacagt tcccaattct atacattcaa taccttgaga ttttagtgat
      901 gcaagtgtat ttgcattggt ttggattaca attgccgggt tttcattgaa taatacttca
      961 acactatttg aatttgtaaa tccttcaata ctaatattca ttccactatg agtatttgca
     1021 aaattcattt cgag
//
LOCUS       DAOTOW010000801         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_120972_length_1029_cov_3.119097, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000801 DAOTOW010000000
VERSION     DAOTOW010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120972_length_1029_cov_3.119097"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="P5243_11365"
     CDS             complement(<1..231)
                     /locus_tag="P5243_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20095.1"
                     /translation="MKTLLYAIFFILFLSSSVYHPIHISYTKIENKGNEIKMLVKIFS
                     DDLTQAVQSYYNKKTISDSDFLNYILTTTKITI"
     gene            complement(237..>1029)
                     /locus_tag="P5243_11370"
     CDS             complement(237..>1029)
                     /locus_tag="P5243_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20096.1"
                     /translation="KIIVISTVVFTLSCNKQEQYGFDENDTIRGIWGQTILYNEYGLP
                     LQNNEGIIVTAHCIDTLGTDNSGNAIVLDTILSTTTDSKGMWQYNNAPGGLYYIEFWK
                     EGFCKNSVYNYKYDTLRADTLDMLYLSQKPQASIIFDSVSVRSSILYLSRTVSFTSSN
                     TNNYMLSTWYFFDTTAQVSSTKHMYAYMSGAAIGKGGIQQSFTTKKPLDKLYENGFKE
                     GTKIYVTCAVDNAKYVQYYDASGTIMFPNISQLSAVKTFIMPKEN"
BASE COUNT          336 a          145 c          167 g          381 t
ORIGIN      
        1 tatagtaatt tttgtagttg taagaatata gtttagaaaa tctgagtcgg aaatagtttt
       61 tttattataa tatgattgta cagcttgtgt taaatcatcg gaaaatattt ttactaacat
      121 ttttatctca ttgcctttat tttcaatctt agtgtatgaa atatgaatag gatgatatac
      181 cgatgatgat aaaaataaaa taaagaatat ggcatacagt agagttttca tctgtattag
      241 ttttctttgg gcattataaa tgttttaaca gcagaaagtt gcgagatgtt tggaaacata
      301 attgtacccg aagcatcata atattgcaca tatttagcat tgtcaacagc acatgttaca
      361 tatatttttg taccttcttt aaagccattt tcatagagtt tgtcaagtgg tttttttgtt
      421 gtaaacgatt gttgtattcc ccctttacca atagcagctc ccgacatata tgcatacatg
      481 tgtttggtac tgcttacttg tgctgtagtg tcaaaaaaat accatgtaga gagcatataa
      541 ttgttggtat ttgatgaagt aaaagataca gtacgtgata aatagagaat agaagaacgt
      601 accgatacag aatcaaaaat aattgaagct tgaggttttt gagataaata caacatatct
      661 aatgtgtcag ctcgtaatgt gtcatatttg taattgtata cagagttttt acaaaatcct
      721 tctttccaaa attctatata atatagccct ccgggagcat tgttgtattg ccacatacct
      781 ttggaatctg tagtagttga aagtatagta tccaatacta tagcattacc cgaattatct
      841 gtacctaatg tatctataca gtgtgctgta acaattatac cttcattgtt ttgtagaggc
      901 aatccatatt cattatataa tatagtttgt ccccaaatac ctcgaattgt atcattttca
      961 tcaaacccgt attgttcttg tttgttacat gatagggtaa aaactacagt acttattaca
     1021 attattttg
//
LOCUS       DAOTOW010000802         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_122179_length_1023_cov_3.502066, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000802 DAOTOW010000000
VERSION     DAOTOW010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122179_length_1023_cov_3.502066"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="P5243_11375"
     CDS             <1..823
                     /locus_tag="P5243_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRS20097.1"
                     /translation="FTYNYIFVPAFTKVDMKKYFTFFISKVLYINIGIIFGVTIIGFI
                     VFKTWLSSYTNHGEYITVPDFSNLDFDAAQIVAKEHNLQLVIIDTVYSKEIKPGAIVN
                     HKPAANSHVKEYRTIYVSINSHTPILVKMPNASNVSLRQATQVLESAGLYVGNIIYKP
                     DFADNYVFEQRYGNRVIPTGAKIPKGSKVDLVVGKGGSTELIDIPNFIGYSYKEVLDS
                     ATVKGVIVNCMFSQEVYASYQDSLMAKVWKQSPAYNQNAKIMTGQIVDVWLNKGE"
     gene            828..>1023
                     /locus_tag="P5243_11380"
     CDS             828..>1023
                     /locus_tag="P5243_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20098.1"
                     /translation="MKSICKFCLFLIFLISYSAYSQSEYISFFELPQSSQLKSLQTNT
                     KKTLVELPFFDDFAKNSYVLS"
BASE COUNT          353 a          132 c          158 g          380 t
ORIGIN      
        1 tttcacatat aattatatct ttgttcctgc gtttacaaaa gttgatatga aaaaatattt
       61 tacatttttc atctctaaag ttttatatat aaacataggg ataatatttg gtgttacaat
      121 aattggtttt attgttttta aaacatggtt atcttcatac actaatcatg gtgaatatat
      181 tacagttcct gatttttcaa atttagattt tgatgcagct caaattgttg caaaagaaca
      241 taatttacag ttggttataa tagatactgt gtattcaaaa gaaataaaac cgggagctat
      301 agtaaatcat aaacctgctg caaattctca tgtgaaggaa tatagaacta tttatgtttc
      361 tattaattct catactccta ttttagtcaa aatgcctaat gcgtctaatg tttcactgcg
      421 tcaagccact caagtgctcg aaagtgcagg attatatgta ggaaatataa tatataaacc
      481 ggattttgca gataattatg tgtttgaaca acgctatggt aatagagtaa tcccaaccgg
      541 agctaaaatt cctaaaggta gtaaggttga tttagttgtt ggtaaaggag gaagtaccga
      601 acttattgat atacctaatt ttattggata ttcatataaa gaagtcttag attctgccac
      661 tgtaaaaggt gttattgtta attgtatgtt ttctcaagaa gtatatgctt catatcaaga
      721 ttctttaatg gcaaaagttt ggaaacaatc acctgcgtat aatcaaaatg ctaaaattat
      781 gacaggacaa atagtagatg tgtggttgaa taaaggagag taggtatatg aaatcaatat
      841 gtaaattttg cttattttta atttttttaa tttcgtattc tgcttattca caatcagaat
      901 atatctcttt ttttgaatta cctcaatctt cacaattaaa atcattgcaa acaaatacaa
      961 aaaaaacatt ggttgaatta cctttttttg atgattttgc aaagaattct tatgtgcttt
     1021 ctt
//
LOCUS       DAOTOW010000803         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_123334_length_1017_cov_3.719335, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000803 DAOTOW010000000
VERSION     DAOTOW010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123334_length_1017_cov_3.719335"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="P5243_11385"
     CDS             complement(<1..909)
                     /locus_tag="P5243_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS20099.1"
                     /translation="MNTISILIIDDAHELMLKKFSDAGFAVTHNEHMSVEEVNQCIHL
                     YDAIIVRSKIIADKSFFDKATKLQCIGRVGAGMETIDILYAESKGIACFNSPEGNRDA
                     VGEQTIGVLLSLMNNVLKADHEVRNLIWEREGNRGIELKGRTVGIIGYGNMGSAFAKK
                     LSGFECTVLAYDKYKTNFSNSYVTEVSLQELQQRADVISFHVPQTPETIYMCNKEFIE
                     ACKKPFILLNTARGKVVNTHDVVEAMKTNKIIAAGLDVLEYESYNFQDFLTDNMPEAF
                     NYLKTSQRVILTPHIAGWTVESKEKLS"
BASE COUNT          307 a          181 c          148 g          381 t
ORIGIN      
        1 tgataatttt tcttttgatt ctactgtcca tcctgcaata tgaggggtaa gtataacccg
       61 ttgcgatgtt tttaaataat taaaagcttc gggcatattg tctgttaaga aatcttgaaa
      121 attataactt tcatattcca aaacatcaag tccggcagct attattttat ttgttttcat
      181 agcttctaca acatcatgag tgtttactac tttacctcgg gcagtgttga gtaaaataaa
      241 aggttttttg caagcttcta taaattcttt gttgcacata tatatagttt cgggggtttg
      301 tggaacatga aaactaataa catcggcacg ttgttgaagt tcttgtaagc tcacttctgt
      361 tacatatgaa ttactaaagt ttgttttata tttatcatac gccaatacgg tacattcaaa
      421 tcctgaaagt ttctttgcaa aagcactacc catattcccg tatcctataa ttcctacggt
      481 tcttcctttt agttcaatac cacggttgcc ttcgcgttcc catattaaat ttcgtacttc
      541 atggtcggct ttcaaaacat tattcattaa tgaaagcaat acacctattg tttgttctcc
      601 tacagcatct ctattgcctt cgggtgaatt gaagcaggca attcctttgg attcggcata
      661 gagaatatct attgtttcca tacctgctcc tacacgtcct atacattgta atttagtagc
      721 tttatcaaaa aaagatttgt ctgcaattat ttttgaacgt acaattatgg catcgtataa
      781 atgaatacac tgatttactt cttctacact catatgttca ttgtgggtaa ctgcaaaacc
      841 tgcgtctgaa aattttttga gcatgagctc atgagcatcg tctattataa gtattgaaat
      901 ggtattcata tgtattttta tcaaaatata tctctattca tgctgtaaaa atacatatta
      961 attatcatta ttgtccgata aaactacaaa attaatttaa taaaacaaaa tttatct
//
LOCUS       DAOTOW010000804         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_123340_length_1017_cov_3.570686, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000804 DAOTOW010000000
VERSION     DAOTOW010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123340_length_1017_cov_3.570686"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5243_11390"
     CDS             <1..183
                     /locus_tag="P5243_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20100.1"
                     /translation="FKLQIYTYFSCPNFGVYYTKKRVSEMQIKIIEENRKYHQILLEA
                     CIAYTYQIQYFESDTH"
     gene            369..>1017
                     /locus_tag="P5243_11395"
     CDS             369..>1017
                     /locus_tag="P5243_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20101.1"
                     /translation="MKKVITIIALSAIVSMANAQLFVGGMAQIVSTGGKTKTEPTSGS
                     PVTVDNNSTFTMSLCPKVGFMMSEKIAVGSNVWLISSTNENPNTNTTNSTGTIGLLPF
                     ARYYVLNGEKFKMFAEAELGLTYSADKTTSNGITTEGDKRIGRVLNVSPNVGYAISPK
                     IELEARLNFVNCIVASNITKKELPNGDLQKITNNSANLKVDFSTIFTTGFLTIGAI"
BASE COUNT          364 a          132 c          165 g          356 t
ORIGIN      
        1 tttaagttac aaatttacac ttatttttcc tgtcctaatt ttggggtgta ttatactaaa
       61 aaacgtgtga gcgaaatgca gattaaaata attgaagaaa atagaaaata tcatcaaatc
      121 ttacttgaag cttgtattgc atatacatat cagattcagt actttgaatc tgatacacat
      181 tagttcaaat attctattac ttacttgata ttttattgta attatttttt ttataaaatt
      241 tattgtttgt gtttaggcat ttcaaaacat ttatatattt gccatgcata agtgaattcc
      301 ccacttatga aaaaatgtat aacaatgttt tatgttggga atgcataaaa tactaaaata
      361 acaatgaaat gaaaaaagta ataacaatta tcgcattgag tgctatagta agtatggcaa
      421 acgcacaatt atttgtagga ggaatggctc agattgtttc taccggagga aaaactaaaa
      481 cagaacctac aagtggttcg cctgttacag ttgataataa ttcaacattt actatgtctt
      541 tgtgtcctaa agttgggttt atgatgtctg aaaaaattgc tgttggttct aatgtatggt
      601 taattagtag tactaatgag aatccaaata caaatacaac taattcaacc ggtactattg
      661 ggttacttcc ttttgctcga tattatgtgt taaatggtga aaaatttaaa atgtttgccg
      721 aagcagaatt aggattaacg tattctgcag ataaaactac ttctaatgga attactacag
      781 aaggagataa acgtatagga cgtgttctta atgtaagtcc aaatgtgggg tatgctatat
      841 cacccaaaat tgaacttgag gctcgtttaa attttgtaaa ttgtatagtt gcatctaata
      901 ttacaaaaaa agagcttcct aatggtgatt tgcaaaaaat tacaaataat tcagcaaatc
      961 taaaagtgga ttttagtaca atctttacta ccggttttct tacaatagga gcaattt
//
LOCUS       DAOTOW010000805         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_123409_length_1017_cov_2.247401, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000805 DAOTOW010000000
VERSION     DAOTOW010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123409_length_1017_cov_2.247401"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1017
                     /locus_tag="P5243_11400"
     CDS             <1..>1017
                     /locus_tag="P5243_11400"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HRS20102.1"
                     /translation="NLGFLVGLNSGYLLMGFLLAGVMHVIISKQTIQRYLGSNSIKSV
                     VYASLLGVPLPLCSCGVLPTGIAFRKQGASAGATVSFLISTPQTGVDSIIATYSLLGL
                     PFAIARPIIAFISGIFGGIITNIFRETEKPKPMFTIDIPKTTCTDNCCCEQSKKQEQK
                     IHPLIRILKYGFIDFIEDIGRWLLIGFVLATIVSVAIPQNFFTTLTTNPILHILFVLL
                     LSVPLYTCATGSIPVAASLLLKGVSPGAALVFLMAGPATSVASVTVLLQSIGKRTTFL
                     YLFSIIFSAVFFGLLIDFILPHNWFSITNTVGVVCEHHTFSVFESVCSVILFILIFYS
                     ILKKW"
BASE COUNT          264 a          169 c          181 g          403 t
ORIGIN      
        1 aaccttggtt ttcttgtagg tcttaattct ggctatttac ttatgggatt tttgcttgca
       61 ggtgttatgc acgtaataat ttcaaaacaa actattcagc gttatttagg ttctaattca
      121 ataaaatcgg tagtatatgc atcgttgctc ggagttccct tgccgttgtg ttcttgtggt
      181 gtgttgccta ccggtatagc atttcgtaaa caaggggcat cggcaggagc tactgtttcg
      241 tttcttattt caacgccgca aacaggtgtt gattctataa tagcaacata ttctttattg
      301 ggattgccat ttgcaattgc tcgtcctatt attgcattta tttcaggcat atttggtgga
      361 attatcacta atattttcag agaaactgaa aagccaaaac caatgtttac tattgatata
      421 ccaaaaacta catgtacaga taattgttgt tgcgaacaat caaaaaaaca agaacaaaaa
      481 attcatccgt tgattcgtat attgaagtat gggtttatag attttattga agatattgga
      541 agatggttgc ttattggatt tgttttggca acaatagttt ctgttgctat tccgcaaaat
      601 ttctttacaa cccttacaac aaatccgatt cttcatattt tgtttgtatt gttactttct
      661 gtgcctttat atacctgtgc tacagggtct atacccgtag ctgcgtctct tttgctcaaa
      721 ggtgtttctc ccggagccgc attagttttt ttaatggcag gtcctgcaac aagtgtcgct
      781 tctgttacag tattattaca atctattggg aaacgtacta cttttttata tcttttttca
      841 attatatttt cggcagtttt ctttggatta ttgattgatt ttattcttcc ccacaattgg
      901 ttttctataa ccaatacggt aggggtggtt tgcgaacatc atacattttc tgtttttgaa
      961 tcggtttgtt ctgttatttt gtttattctt atattctatt caatattaaa aaaatgg
//
LOCUS       DAOTOW010000806         1016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_123564_length_1016_cov_2.681582, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000806 DAOTOW010000000
VERSION     DAOTOW010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123564_length_1016_cov_2.681582"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..936)
                     /locus_tag="P5243_11405"
     CDS             complement(103..936)
                     /locus_tag="P5243_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRS20103.1"
                     /translation="MVAIGKINTLEVYKFVDFGAYLDGEQYGPILLPKKYMPQGLEIE
                     QTIEVFVYLDSEDRLIATTETPKAQVGDFAYLCVKDVNDYGAFLDWGLSKDLFVPYKE
                     QKNKMTVGSYYWVYVYIDSKTERIVATSKIGRYISEQKPQYNIGDQVHVIVHSQTDLG
                     YKVIVDKSYWGILYANELFEPVEIAEQRVGYVHVVRDNNKLDIRLQKSGFQKMPDVQK
                     KILTLLKQHNGFLPFHDKSSPQEIQTMFAMSKKTFKMAIGILYKGGYIHILENGISLV
                     Q"
BASE COUNT          335 a          174 c          121 g          386 t
ORIGIN      
        1 caaatataga atttttttaa aataacgata catgaatttt taagagttta taatgatttt
       61 tcttacaatt tcaacataat ttcaaaatca caactattcg tattattgaa ctaatgaaat
      121 tccgttttca agtatgtgta tatatccacc tttatataaa attcctatag ccattttaaa
      181 tgttttttta ctcatagcaa acattgtttg aatttcttgt ggacttgatt tatcatgaaa
      241 aggtaaaaat ccgttgtgtt gcttgagaag agtaagtatt ttcttttgaa catcgggcat
      301 tttttggaaa ccactttttt gcaaacgtat atcaagctta ttattatcgc gtaccacatg
      361 cacatatcct acacgttgtt ctgctatttc aactggttca aacaattcat ttgcatatag
      421 aattccccaa tatgatttat ctactattac tttataaccc aagtctgttt gtgaatgtac
      481 gataacatgt acttggtcac caatgttgta ctgaggtttt tgctccgaaa tatatctgcc
      541 aattttagaa gtagcaacta tacgttctgt tttactgtca atatacacat atacccaata
      601 ataagaacca actgtcattt tatttttctg ttctttatat ggaacaaaca aatcttttga
      661 taaaccccaa tctaaaaaag caccataatc atttacatct tttacacata aatatgcaaa
      721 atcacctacc tgtgctttag gagtttcggt agttgcaatt aatctgtctt cggaatctaa
      781 atacacaaaa acctctattg tttgttctat ctcaagtcct tgcggcatat atttttttgg
      841 taataaaatt ggaccatact gctcgccatc gagatatgcc ccaaaatcaa caaatttata
      901 tacttcaagt gtatttattt ttcctatagc aaccatgtaa gtatttttta ttatttttga
      961 caaacataca aaaaaaagct ttgtatgttt ttaataaatt aacacaataa tcattt
//
LOCUS       DAOTOW010000807         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_125484_length_1007_cov_2.436975, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000807 DAOTOW010000000
VERSION     DAOTOW010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125484_length_1007_cov_2.436975"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1007)
                     /locus_tag="P5243_11410"
     CDS             complement(<1..>1007)
                     /locus_tag="P5243_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20104.1"
                     /translation="GPVYIGAIVVFLFIFGLLYVQGTIKWWLLTITIFSILLAWGKNF
                     MGLTEFFLDHFPGYNKFRTVSMILIIAQFAMPLLAILALKRFFTDEESRTDAFKKLKI
                     SLIVTGGFLLILLITVGGIYDFSGQYDSQMLPDWLLDALRNDRKSMLYSDIWRTLFFV
                     VATFGVLTAVYFKKITVVVAITLLTVFTYFDMWGVNKRYLNDQDFVAKRSVTNPFPKS
                     SADVQILADTDPNFRVMNLTVSPFNDASTSYYHKSIGGYHGAKMKRYQELIDFHLSKM
                     NMSVYNMLNTKYFIVEDQQTKQPIAQRNPDALGNAWFISKVTIVPNADAEIAALEKFD
                     P"
BASE COUNT          356 a          172 c          156 g          323 t
ORIGIN      
        1 ttaggatcaa atttttctaa agctgcaatt tcagcatcgg cgttgggaac tatagtaact
       61 ttagaaataa accaagcatt acccaaagca tcaggattgc gttgtgcaat aggttgtttt
      121 gtttgttggt cttcaactat aaaatatttt gtattaagca tattatatac actcatattc
      181 attttgctta aatggaaatc aattaattct tgatatcgtt tcattttagc accatgatat
      241 ccacctatag atttatgata atatgatgta cttgcatcat taaatgggct tacagtaagg
      301 ttcataactc taaaattagg gtcagtatcg gctaaaattt gcacatcggc cgatgatttt
      361 ggaaaaggat tagtaacaga acgctttgca acaaaatctt gatcattgag atatcgtttg
      421 tttactcccc acatatcaaa ataggtaaac actgtaagta atgtaattgc tacaactact
      481 gtaatttttt taaaatatac tgctgttaat acaccaaatg ttgcaactac aaaaaataat
      541 gttctccaaa tatcgctgta caacatgctt ttacggtcgt tgcgtaaagc atcaaggagc
      601 cagtcgggta acatttgtga atcatattgt ccgctaaaat catatatgcc acctacagta
      661 attaataaaa taagaagaaa acctccggtg acaattagag aaatctttaa ttttttaaaa
      721 gcatctgttc tactttcttc atcggtaaaa aagcgtttaa gtgcaagtat tgcaagtagt
      781 ggcattgcaa attgtgcaat tataagaatc atagacactg ttctgaattt attatatccc
      841 gggaaatgat ctaaaaagaa ttcggttaac cccataaagt ttttacccca agccagcaat
      901 attgaaaata tggttatagt gagcaaccac cattttatgg taccttgaac atacaataaa
      961 ccaaaaataa acaaaaatac tactatagca cctatataaa ccggtcc
//
LOCUS       DAOTOW010000808         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_126063_length_1004_cov_2.769231, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000808 DAOTOW010000000
VERSION     DAOTOW010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126063_length_1004_cov_2.769231"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1004)
                     /locus_tag="P5243_11415"
     CDS             complement(<1..>1004)
                     /locus_tag="P5243_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS20105.1"
                     /translation="YPGFYIDNIKRSIPRHKEVITNATQYKITDNDTIIAIVNNYFDV
                     TRKDSANVYFKVMPPGKGAKNITVGPICATNGNQYVGFDLTQYNYEVGGALVADKNWF
                     ANQALTIPITNPKNVTVTGTQTFWAQLDDECGGVGSLTIQVSPQPNVMNATEQICENV
                     DIGGDRGILDLSAMKNRVTTNLDATIVWYSDAARTTLVPNINQVFPVDGQKYYANIYY
                     NPTCIAQATLTVTVIPLSDVTFPNRSVCIDGGNVIMNATPAGGVYTGPGVTGNIFNPV
                     TAGAGTHEISYTISNLSCNYTKKANYTVHPLVVASITQVPAGKLQVNGTATLQGAITP
                     "
BASE COUNT          301 a          191 c          173 g          339 t
ORIGIN      
        1 ccggagttat agcaccttgt aaggtagcgg taccatttac ttgtaattta cctgcaggta
       61 cttgtgttat agaagctaca actaacggat gaacagtata attggccttt ttggtataat
      121 tacaagataa attagatata gtgtatgaaa tttcatgcgt acctgcaccg gcagttacag
      181 gattgaatat attacctgtt acacccggcc cggtatatac accaccggca ggtgttgcgt
      241 tcattattac attaccaccg tcaatacata cacttctatt agggaatgtt acatcactca
      301 atggaattac cgtaacagta agagttgcct gagctataca cgtaggatta taataaatat
      361 ttgcataata tttttgtcca tcaaccggga atacttggtt aatatttgga accaaagtag
      421 tacgtgcagc atctgaatac catacaatag tagcatctaa attggtagtt acacgatttt
      481 tcattgcact taaatcgagt atccctctat cgccgcctat atctacattt tcacaaattt
      541 gttcggtagc attcattaca tttggttgcg gcgagacttg tatggtaagc gaccctaccc
      601 caccacactc atcatctaat tgagcccaga atgtttgcgt tccggttacg gttacatttt
      661 taggatttgt tataggaatg gttaacgcct gatttgcaaa ccaattttta tcggcaacta
      721 atgctcctcc aacctcgtaa ttatattggg ttaaatcaaa accaacatac tgatttccat
      781 ttgtagcaca aataggtcca acagtaatat ttttagctcc ttttccggga ggcattactt
      841 tgaaatatac atttgctgaa tctttacgag taacatcaaa ataattattt acaattgcaa
      901 taattgtgtc gttatcagta attttatatt gtgttgcatt ggttattact tctttatggc
      961 gaggtatact acgttttata ttatcaatat aaaatccagg atat
//
LOCUS       DAOTOW010000809         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_126502_length_1002_cov_3.667371, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000809 DAOTOW010000000
VERSION     DAOTOW010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126502_length_1002_cov_3.667371"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="P5243_11420"
     CDS             complement(<1..387)
                     /locus_tag="P5243_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRS20106.1"
                     /translation="MSEKIYNNITELIGNTPLLRLQSPANFASEIIGKLEYFNPGSSV
                     KDRIGFAMIDKAEKAGVLKPGATIIEPTSGNTGIGLALTAAVKGYKIIFTMPESMSIE
                     RRKILESLGAQIVLTDAQKGMPGAIEQ"
     gene            complement(456..851)
                     /locus_tag="P5243_11425"
     CDS             complement(456..851)
                     /locus_tag="P5243_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRS20107.1"
                     /translation="MIQIQSHIAQAIIDYARSKLPIEACGYLAGNNGLITEWYGLTNI
                     DNSPEHFSFDPKEQFATVKDARSKGLQIIANFHSHPETPARPSQEDIRLAFDPNILYF
                     IISFAEETPDIKAFKIINGEVEKIEIQIL"
     gene            complement(848..976)
                     /locus_tag="P5243_11430"
     CDS             complement(848..976)
                     /locus_tag="P5243_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRS20108.1"
                     /translation="MEVTLKAGEPLENVPRSAAEQGFTVLETKQLHDDTYQVTIQK"
BASE COUNT          267 a          189 c          187 g          359 t
ORIGIN      
        1 ttgttctata gcccccggca tacctttttg cgcatcggta agcactattt gagcaccaag
       61 cgattccaat attttgcgac gttctatact catactttct ggcatagtaa atataatttt
      121 atagcctttt accgcagccg taagagccag tcctattccg gtattccctg aggttggttc
      181 tatgatagta gcaccgggtt tcaatactcc agccttttcg gctttgtcta tcatagcaaa
      241 gcctatgcgg tcttttaccg aagaaccggg attaaaatat tctaattttc caataatttc
      301 tgatgcaaaa tttgcaggtg attgcaatcg caaaagaggt gtgtttccaa ttaattcggt
      361 tatgttattg tatatttttt ctgacatatt tgtaatagta tgtatactaa aaattattgg
      421 tgcaagataa aaaaaacttt caaatcctac tgcttttata ggatttgaat ttcaattttt
      481 tcaacttctc cgtttattat tttaaatgct ttaatatcag gcgtttcttc ggcgaacgat
      541 ataataaaat acaatatgtt cgggtcaaat gccaatcgaa tatcttcttg cgatgggcgt
      601 gccggcgttt cggggtggct atggaagttt gcaataattt gcaatccttt actacgagca
      661 tcttttactg ttgcaaattg ctctttagga tcaaacgaaa aatgttcggg gctattgtca
      721 atattggtaa gtccatacca ctcggttata agcccattgt tacctgctaa atatccgcat
      781 gcttctatgg gcaatttact gcgagcatag tcaataattg cttgtgcaat atgtgattgt
      841 atctgtatca tttttgtata gttacttggt aagtatcatc atgtaattgt ttagtttcga
      901 gcacagtaaa accttgttct gctgcactgc gaggaacatt ttctagaggt tcgcctgctt
      961 tgagggttac ctccaaaata tcaccttgag ctatttgtgc ca
//
LOCUS       DAOTOW010000810         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2859
            NODE_126754_length_1001_cov_2.886892, whole genome shotgun
            sequence.
ACCESSION   DAOTOW010000810 DAOTOW010000000
VERSION     DAOTOW010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867883
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:33:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,287
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 21
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 4 of 13
            Pseudo Genes (incomplete)         :: 5 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126754_length_1001_cov_2.886892"
                     /isolate="AMR_MDS_2859"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="P5243_11435"
     CDS             <1..925
                     /locus_tag="P5243_11435"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRS20109.1"
                     /translation="DQNIYRIGDYKQNLDFVRENVLVQSDDNLLTALLTRTPNKAVYF
                     VKKGILAYEQQLTPNIIIKPSYSFSQHYNPPYYNFDTVTQLQSFGVHEFGTNIRVSFK
                     EEISNNHFRKIYIDSRYPIFHFNIVQGKYILPYTQGWFTQMRFVTRQDILIGIGRMRY
                     VVESGITTKPVPFPMLEFHRGNETGGSGEYYFNHMKYLEFASDRFVNVYAEYGMNGFF
                     FNKIWLINKLNLRELLTFKACWGQLSQNHASIFSLPANTYELKAPYMEAGIGVTNFLK
                     VIRIEYIWRLNYLDHPDVRTGGLYFRFQFEF"
BASE COUNT          347 a          138 c          163 g          353 t
ORIGIN      
        1 tgatcagaat atatatagaa ttggcgatta taaacaaaat cttgatttcg ttcgtgaaaa
       61 tgtattggtt caatccgatg ataatttatt aacagctcta cttactcgca caccaaataa
      121 agcggtatat tttgtaaaaa aaggaatact tgcctatgaa caacaactta cccctaatat
      181 aattataaaa ccatcgtata gtttttcaca acattataac ccaccgtatt ataattttga
      241 caccgtaact caattacaat cgtttggtgt gcatgaattt ggaactaata ttcgtgtttc
      301 attcaaagaa gagatttcaa acaatcattt tcgtaaaatc tatattgatt cacgatatcc
      361 tatttttcat tttaatatag tacaaggaaa atatatactt ccttatacac aagggtggtt
      421 tacacaaatg cgttttgtaa ctcgtcaaga tattttaata ggtattggta gaatgcgtta
      481 tgttgttgaa tcgggtatta caactaaacc ggttcctttt ccaatgttag aatttcatag
      541 aggaaatgaa acaggtggga gtggggaata ttattttaat catatgaagt atttggaatt
      601 tgcaagtgac agatttgtaa atgtatatgc agaatatggt atgaatggct ttttctttaa
      661 taaaatatgg ctcattaata aattaaacct acgcgaattg ttaaccttta aagcatgttg
      721 gggacaatta tctcaaaatc atgcttctat ctttagttta cctgcaaata catatgaatt
      781 aaaagctcct tatatggaag caggtattgg cgtaacaaat tttttaaagg ttattcgaat
      841 agaatacatt tggcggttaa attatcttga ccaccccgat gtaagaaccg gaggtttata
      901 ctttagattc caatttgaat tttagtttag aatatattat atacataagg taaatggctg
      961 tcttttttga caagttgtaa atatagtaaa atgatttgag a
//
